LOCUS       DAWISC010000001        49771 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWISC010000001 DAWISC010000000
VERSION     DAWISC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.001"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WIW87_000001"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WIW87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ5173233.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2320..2410)
                     /locus_tag="WIW87_000002"
     tRNA            complement(2320..2410)
                     /locus_tag="WIW87_000002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2374..2376),aa:Ser,seq:gga)
     gene            complement(2449..2535)
                     /locus_tag="WIW87_000003"
     tRNA            complement(2449..2535)
                     /locus_tag="WIW87_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2499..2501),aa:Leu,seq:cag)
     gene            complement(2571..2657)
                     /locus_tag="WIW87_000004"
     tRNA            complement(2571..2657)
                     /locus_tag="WIW87_000004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2621..2623),aa:Leu,seq:cag)
     gene            2746..5122
                     /gene="rnr"
                     /locus_tag="WIW87_000005"
                     /pseudo
     CDS             2746..5122
                     /gene="rnr"
                     /locus_tag="WIW87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(5461..6924)
                     /gene="guaB"
                     /locus_tag="WIW87_000006"
     CDS             complement(5461..6924)
                     /gene="guaB"
                     /locus_tag="WIW87_000006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ5173234.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7125..7205)
                     /locus_tag="WIW87_000007"
                     /pseudo
     CDS             complement(7125..7205)
                     /locus_tag="WIW87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(7251..7712)
                     /locus_tag="WIW87_000008"
     CDS             complement(7251..7712)
                     /locus_tag="WIW87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173235.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(7803..10361)
                     /gene="glnD"
                     /locus_tag="WIW87_000009"
     CDS             complement(7803..10361)
                     /gene="glnD"
                     /locus_tag="WIW87_000009"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ5173236.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(10410..10730)
                     /locus_tag="WIW87_000010"
     CDS             complement(10410..10730)
                     /locus_tag="WIW87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5173237.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(11055..11528)
                     /gene="bfr"
                     /locus_tag="WIW87_000011"
     CDS             complement(11055..11528)
                     /gene="bfr"
                     /locus_tag="WIW87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ5173238.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(11556..12020)
                     /gene="bfr"
                     /locus_tag="WIW87_000012"
     CDS             complement(11556..12020)
                     /gene="bfr"
                     /locus_tag="WIW87_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ5173239.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            12375..12578
                     /locus_tag="WIW87_000013"
     CDS             12375..12578
                     /locus_tag="WIW87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173240.1"
                     /translation="MPSEGLSDGMPARTDAAWSGCRFEGVFNAKGNLSYDVFAAGALK
                     KPGILGRSARLPGSISAIYSKPY"
     gene            12596..13063
                     /locus_tag="WIW87_000014"
     CDS             12596..13063
                     /locus_tag="WIW87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ5173241.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(13104..13459)
                     /locus_tag="WIW87_000015"
                     /pseudo
     CDS             complement(13104..13459)
                     /locus_tag="WIW87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13456..13794)
                     /locus_tag="WIW87_000016"
     CDS             complement(13456..13794)
                     /locus_tag="WIW87_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173242.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(13791..14153)
                     /locus_tag="WIW87_000017"
     CDS             complement(13791..14153)
                     /locus_tag="WIW87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ5173243.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(14150..20971)
                     /locus_tag="WIW87_000018"
     CDS             complement(14150..20971)
                     /locus_tag="WIW87_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ5173244.1"
                     /translation="MNKRCYKVIFNKKRSCMMAVAENVHRDGKSMQDSEAASVRVTGA
                     ASVSSARAAFGFRMAAFSVMLALGVAAFSPAPASGIIADKSAPKNQQAVILQTANGLP
                     QVNIQTPSSQGVSVNRFKQFDVDEKGVILNNSRSNTQTQLGGWIQGNPHLARGEARVI
                     VNQIDSSNPSLLNGYIEVGGKRAEVVVANPSGIRVNGGGLINAASVTLTSGVPVLNNG
                     NLTGFDVSSGKVVIGGKGLDTSDADYTRILSRAAEINAGVWGKDVKVVSGKNKLDFDG
                     SLAKTASAPSSSDSVTPTVAIDTATLGGMYADKITLISTDNGAVIRNKGRIFAATGGV
                     TLSADGKLSNSGSIDAAEITISAQTVDNRQGFIRSGKGSVLKVSDGINNQAGLIGSAG
                     LLDIRDTGKSSLHINNTDGTIIAGKDVSLQAKSLDNDGILTAARDVSVSLHDDFAGKR
                     DIEAGRTLTFSTQGRLKNTRIIQAGDTVSLTAAQIDNTVSGKIQSGNRTGLNGKNGIT
                     NRGLINSNGITLLQTEAKSDNAGTGRIYGSRVAVEADTLLNREETVNGETKAAVIAAR
                     ERLDIGAREIENREAALLSSSGDLHIGSALNGSRQVQGANTSLHNRSAAIESSGNIRI
                     ATKDLQNTNEHLRFHTEETHREHRIEYQAEGRTERYPEGSQKELGWEIFEDESLHMRT
                     PDGSPHSVWYKYDYERITAESKITESKPGQIISGGNLVLDAAKLKNHNSRIIAGGRLI
                     VGTPESALDNDETLGTKTITDKGDLHRYHRHHKKGRDSTGYSRSPYEPAPEVSSIRMG
                     ISAYKGYAPQQASDIPGTVVPVVAENGIHPTFTLPNSSLFAIAPNNKGYLIETDPAFT
                     DYRKWLGSGYMLAALQQDPNHIHKRLGDGYYEQKLVNEQIAKLTGYRRLDGYTNDEEQ
                     FKALMDNGITIAKELQLTPGIALSAEQVARLTSDIVWLENETVTLPDGTTQTVLKPKV
                     YVRARPKDMNGQGALLSGSVVDIGSGAIENRGGLIAGREALILNAQNIKNLQGDLQGK
                     NIFAAAGSDITNTGSIGAENALLLKASNNIESRSETRSNQNEQGSVRNIGRVAGIYLT
                     GRQNGSVLLDAGNNIVLTASELTNQSEDGQTVLNAGGDIRSDTTGISRNQNTIFDSDN
                     YVIRKEQNEVGSTIRTRGNLSLNAKGDIRIRAAEVGSEQGRLKLAAGRDIKVEAGKAH
                     TETEDALKYTGRSGGGIKQKMTRHLKNQNGQAVSGTLDGKEIILVSGRDITVTGSNII
                     ADNHTILSAKNNIVLKAAETRSRSAEMNKKEKSGLMGSGGIGFTAGSKKDTQTNRSET
                     VSHTESVVGSLNGNTLISAGKHYTQTGSTISSPQGDVGISSGKISIDAAQNRYSQESK
                     QVYEQKGVTVAISVPVVNTVMGAVDAVKAVQTVGKSKNSRVNAMAAANALNKGVDSGV
                     ALYNAARNPKKAAGQGISVSVTYGEQKNTSESRIKGTQVQEGKITGGGKVSLTASGAG
                     KDSRITITGSDVYGGKGTRLKAENAVQIEAARQTHQERSENKSAGFNAGVAIAINKGI
                     SFGFTAGANYGKGYGNGDETAYRNSHIGSKDSQTAIESGGDTVIKGGQLKGKGVGVTA
                     ESLHIESLQDTAVFKGKQENVSAQVTVGYGFSVGGSYNRSKSSSDYASVNEQSGIFAG
                     GDGYRIRVNGKTGLVGAAVVSDADKSKNLLKTSEIWHKDIQNHASAAASALGLSGGFS
                     YSPKPTSGQYSTKKEAEIGKIGGKPVSLMRFDQVSAKDDELNEKYRSERIEKGETFKE
                     ANLNQNNAGGLKFGLKQNDIHSNDKYALAKMGLGNLLGNAKESESRQSITHSVISEGD
                     WQIASAQGRKNIAGIEKGTSSAHKALAKADREGLLKEVELNRDVAKEFINETLIGGIA
                     DEAYRSQFIAEHRLMTFKMDENGEPIEDKQLEEDINKQFDNSVKLKKEFASFKDYWEA
                     YKAIGGNIYELREVSDQERKNLKTARYTDPETGKTVEKIVVGVNGIFNNIQAAAKFAA
                     QQYVGRFNPEKNRYERTYENVYFLHNPETNGRGFSKLPEIAVAAFHKMLEGAKIGNKT
                     VIGLSNSGLALGNIMEDYGKDKNGLFVGSHSRGTLVVDNVLNTLNTQANRDKKILSNT
                     ELKMVGPAANVVRADKRLFQLQQGVTTPRTADFARQSIQIENHELDLIGMLIGRNPAT
                     VGTNTRQKSQWQAIRDIIGDYTSPHNCYGMANKQCVTDGYRDPENKQTQSPTGVFERG
                     VSNEIEIMYRPVRIYDLQHPKGKTK"
     gene            complement(21481..22464)
                     /gene="lipA"
                     /locus_tag="WIW87_000019"
     CDS             complement(21481..22464)
                     /gene="lipA"
                     /locus_tag="WIW87_000019"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ5173245.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(22457..23080)
                     /gene="lipB"
                     /locus_tag="WIW87_000020"
     CDS             complement(22457..23080)
                     /gene="lipB"
                     /locus_tag="WIW87_000020"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ5173246.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(23082..23357)
                     /locus_tag="WIW87_000021"
     CDS             complement(23082..23357)
                     /locus_tag="WIW87_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ5173247.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            23542..24612
                     /locus_tag="WIW87_000022"
     CDS             23542..24612
                     /locus_tag="WIW87_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ5173248.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(24809..25756)
                     /locus_tag="WIW87_000023"
     CDS             complement(24809..25756)
                     /locus_tag="WIW87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ5173249.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(25771..26178)
                     /locus_tag="WIW87_000024"
     CDS             complement(25771..26178)
                     /locus_tag="WIW87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ5173250.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            26259..26918
                     /gene="ung"
                     /locus_tag="WIW87_000025"
     CDS             26259..26918
                     /gene="ung"
                     /locus_tag="WIW87_000025"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ5173251.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            27021..27668
                     /locus_tag="WIW87_000026"
     CDS             27021..27668
                     /locus_tag="WIW87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ5173252.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            27741..28214
                     /locus_tag="WIW87_000027"
     CDS             27741..28214
                     /locus_tag="WIW87_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ5173253.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            28562..29506
                     /locus_tag="WIW87_000028"
     CDS             28562..29506
                     /locus_tag="WIW87_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5173254.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            29648..29782
                     /locus_tag="WIW87_000029"
     CDS             29648..29782
                     /locus_tag="WIW87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ5173255.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(30072..30587)
                     /locus_tag="WIW87_000030"
     CDS             complement(30072..30587)
                     /locus_tag="WIW87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173256.1"
                     /translation="MKSKLLLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(31053..32975)
                     /locus_tag="WIW87_000031"
     CDS             complement(31053..32975)
                     /locus_tag="WIW87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5173257.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAETTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGTIDLLSGSIVRSEKLNIGYFAQHQLDTIRSDQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAVAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEEAYFEENKEKLQDTLSELAKVKTQLAQIEEVWLACQE
                     ELEQIETEIEKQFAER"
     gene            complement(33052..33447)
                     /locus_tag="WIW87_000032"
     CDS             complement(33052..33447)
                     /locus_tag="WIW87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ5173258.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            33620..34927
                     /locus_tag="WIW87_000033"
     CDS             33620..34927
                     /locus_tag="WIW87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ5173259.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            34920..35354
                     /locus_tag="WIW87_000034"
     CDS             34920..35354
                     /locus_tag="WIW87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173260.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(35413..35682)
                     /locus_tag="WIW87_000035"
     CDS             complement(35413..35682)
                     /locus_tag="WIW87_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ5173261.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(35864..38326)
                     /gene="lon"
                     /locus_tag="WIW87_000036"
     CDS             complement(35864..38326)
                     /gene="lon"
                     /locus_tag="WIW87_000036"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ5173262.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPIAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGNDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     IHVPEGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            38426..38602
                     /locus_tag="WIW87_000037"
     CDS             38426..38602
                     /locus_tag="WIW87_000037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ5173263.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVSHVFFYFSR"
     gene            38577..39680
                     /locus_tag="WIW87_000038"
                     /pseudo
     CDS             38577..39680
                     /locus_tag="WIW87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(39708..41453)
                     /gene="recD"
                     /locus_tag="WIW87_000039"
     CDS             complement(39708..41453)
                     /gene="recD"
                     /locus_tag="WIW87_000039"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ5173264.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSWLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(41521..42216)
                     /gene="lolD"
                     /locus_tag="WIW87_000040"
     CDS             complement(41521..42216)
                     /gene="lolD"
                     /locus_tag="WIW87_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ5173265.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(42209..43456)
                     /locus_tag="WIW87_000041"
     CDS             complement(42209..43456)
                     /locus_tag="WIW87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ5173266.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            43661..43939
                     /locus_tag="WIW87_000042"
     CDS             43661..43939
                     /locus_tag="WIW87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173267.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(43995..44612)
                     /gene="recR"
                     /locus_tag="WIW87_000043"
     CDS             complement(43995..44612)
                     /gene="recR"
                     /locus_tag="WIW87_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ5173268.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(44679..46217)
                     /locus_tag="WIW87_000044"
     CDS             complement(44679..46217)
                     /locus_tag="WIW87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ5173269.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(46277..46651)
                     /locus_tag="WIW87_000045"
     CDS             complement(46277..46651)
                     /locus_tag="WIW87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ5173270.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            46741..48429
                     /locus_tag="WIW87_000046"
     CDS             46741..48429
                     /locus_tag="WIW87_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ5173271.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            48501..49754
                     /locus_tag="WIW87_000047"
     CDS             48501..49754
                     /locus_tag="WIW87_000047"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ5173272.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT        11234 a        12776 c        13077 g        12684 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgtagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgaccttg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggttatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcagggttga
      601 gcggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggcatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc caatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaggct
     2161 gaggaagagg gtaaaacata ccgcaaaaca taccacaaaa aacggtaacg gatagttgta
     2221 agcggttgat gacgattata gacaataacg ggttttccca atgaaattat tgtttgaata
     2281 ataaaaaatc ccaaaccgta aaagtttggg atttgtattt ggcagagagg aagggattcg
     2341 aaccctcgat acgctattca cgtatacacg ctttccaggc gtgcgactta aaccactcat
     2401 ccacctctct aatggcggaa attatcccaa tcgggataat ttattatttg gtgcccggga
     2461 gaagactcga acttccacac ccatgaggat accagcacct gaagctggcg cgtctaccaa
     2521 ttccgccacc cgggcaatct aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg
     2581 gagaagactc gaacttccac acccgtgagg atactagcac ctgaagctag cgcgtctacc
     2641 aattccgcca cccgggcttt gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa
     2701 gaagtcgcta ttatatagtt cataaagaga atgtcaacag tccaaatgaa taaaaatatt
     2761 aaatctttaa atttacggga aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa
     2821 catcctttgc ccagtcggga atggataatc gaattgttgg agcgcaaagg tgtgccttca
     2881 aaaatcgaat cgcttgcgcg cgagctgtcg attacggaag acgagtatgt cttttttgaa
     2941 cgccgtctga aggcgatggc gcgggacggt caggttttaa tcaaccgtcg gggcgcggtt
     3001 tgcgcggcgg acaaattgga tttggtcaaa tgccgcgtcg aggcgcataa ggacggtttc
     3061 ggttttgccg tgccgctcac gcccgccaaa gacggtgatt ttgttttgta tgaacgccag
     3121 atgcgcggca ttatgcacgg cgatattgtc actgttcgtc ctgccggcat ggaccgtagg
     3181 ggccgccgcg aagggacagt tctggatatt gtcgaacgcg cgcaaagcaa agtggtcggc
     3241 cgtttctata tggatagggg cgtggcgatt ttggagccgg aagacaagcg tctgaaccaa
     3301 agcatcgtat tggaaccgga cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc
     3361 gtcggcgaaa ttgaggttta tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa
     3421 gttttgggcg attatgccga cagcggcatg gagattgaaa ttgccgtgcg caagcatcat
     3481 ttgccgcacc aattcagtga agcgtgtgcc aaagctgcga aaaaaattcc cgtccatgta
     3541 cgcaaaagcg atttgaaagg ccgcgtcgat ttgcgcgacc tgcctttggt aacgatagac
     3601 ggcgaaacgg cgcgcgattt cgacgacgcg gtgtttgccg aaaaagtcgg acgcaattac
     3661 cgtctggtcg tggcgattgc ggatgtcagc cattatgtcc gccctgacga tgtgattgat
     3721 gcagatgctc aagaacgcag taccagcgta tatttcccgc gccgtgtgat tccgatgctg
     3781 ccggaaaacc tgtctaacgg catttgctcg ctcaatcccg atgtcgagcg tttgtgtatg
     3841 gtgtgcgata tggtcgttac ctatgcgggc aatatcaaag aataccgctt ctaccccgcc
     3901 gtaatgcgct ctcatgcccg cctgacctac aaccaagttt ggaaatggat ttcagacggc
     3961 atcgaccatc cgtacaaagc ccaaatcgac accctttaca aactcttcaa aatccttcag
     4021 aaaaagcgtt tcgaacgcgg cgcggtggag tttgaaagcg tcgaaaccca gatgattttc
     4081 gatgacaacg gcaaaatcga aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg
     4141 attgaagaat gtatgctggc ggcgaatgtt tgcgcagcgg atttcctgtt gaaaaacaag
     4201 catacggctt tgttccgcaa ccatttgggc cccacgcccg aaaaactcgc caccctgcgc
     4261 gagcagctcg gtctgttggg gcttcaactt ggcggcggcg acaacccgtc gccgaaagac
     4321 tatgccgcgc ttgtcgaaca attcaaaggc agacctgatg ccgaattgct gcaagtcatg
     4381 atgttgcgct ccatgcagca ggcggtttac gaaccgcatt gcgacggaca ctttggtctt
     4441 gcctacgaag catacgccca cttcacctcg cccatccgcc gctatcccga cctgaccgta
     4501 caccgcgcca tcaaagccgt gttgaatcag caaacctaca cgccaaaaaa aagctggcag
     4561 gctttgggcg tgcatacctc gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg
     4621 gaaaactggc tgaaaaccta ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa
     4681 atctccggca tgaccagttt tggtatcttt gtaacactgg acggcatcca cattgacggc
     4741 ttggtgcata tcagcgattt gggcgaagac tatttcaact tccgccccga aatcatggca
     4801 atcgaaggcg aacgcagcgg catccgtttc aacatggggg acagggttgc cgtccgggtc
     4861 gcccgtgccg atttggatga cggaaaaatc gattttgtcc tgattgccgg gggggagcgg
     4921 cagggggcgg aaagttaaat catccgcgtc tgccaaaccg gcagggacgg cggggaaagg
     4981 gaagccgaaa accgccgccg agaaaaaaac agcccgaggc ggcaaagtaa ggggaagggg
     5041 cgcgtctgcc gccgcagaat cgaggaaaaa ggcaaagaaa ccggttccga ttaaggtaaa
     5101 aaaacggaaa ggcaaatcat aatgctgacg gggtggcttg aggaggcggg gcatattttc
     5161 ccgaagcctc cacccgattt tttgatgtgg gcggacggag cgttatttcc agcaaattga
     5221 aacgcccgta ttgaaagatt gcgtttattt ccaccgccgt tttaaaggcc ggcggtattc
     5281 ggcagacggg gcgcaaacgg cgttcagacg gcattttcat tctttcggcg tgtccgtccg
     5341 aattgctttg cccgtccgcg caatcagccc gtgcggcttg ccttgaacgg acaaaaaatg
     5401 ccgtctgaaa cccgaaaatc aggtttcaga cggcattttt ccttgaaaag gctgttcaaa
     5461 tcagcgatgg tagttcggtg cttctttggt aatttgaacg tcgtgaacgt gcgattcgct
     5521 catacctgcg gaagtgattt ccacaaattc tgctttttcg tgcatttcgg caatattggc
     5581 gcaacccaaa taccccatgc tggagcgcag tccgccggtc agttggtgga tgatgttcac
     5641 aatcgggcct ttgtaaggaa cgcggccttc gatgccttcg gggacgtatt tgtcggtgct
     5701 gtcggttttg tcttggaagt agcggtcggc agaaccttgg ctcatcgcgc ccaaggaacc
     5761 cataccgcga taggatttgt atgagcggcc ttggtagagt tcgatttcgc ccggcgcttc
     5821 ttccgtgcct gcaaacatac cgccgagcat gacgctgtac gcgcctgcgg cgagggcttt
     5881 ggcgatgtcg ccggagaagc ggatgccgcc atcggcaatc agcggaacgc ccgtgccttt
     5941 gagggcttcg gcaacgttgt gaatggcggt cagttgcggc acgccgacac ctgccacgat
     6001 acgggtggtg caaatcgatc ccggaccgat accgactttg acggcatccg cgccggcggc
     6061 gaccaaatcc aaagcggctt tggcagtggc gatgttgccg ccgatgactt ggatgtgcgg
     6121 ataggtttct ttgacccaac gcacgcggtc gatcacgcct tggctgtgcc cgtgggcggt
     6181 atcgacgaca atcacgtcca cgccggcctc aaccaaggct ttgacgcgct cttcggtgtc
     6241 gccgccggtg ccgactgccg caccgacgcg cagacggcct tcggagtctt tgttggcatt
     6301 gggaaactcg gtggttttta aaatatcttt gacggtaatc agacctttga gttcgtcttt
     6361 ttcgttcaga accaaaacgc gctcgacttt gtgcgtgtgc atcagttcgc gcgcttcgtc
     6421 tatgcttgtg ccttcgggga cggtaaccag acgttcgcgc ggggtcataa tggcggaaac
     6481 gggcaaatcg acgcggtttt caaaacgcag gtcgcggttg gttacgatgc cgacgacttt
     6541 gccgttttca acgacgggca ggccggacat tttgcgtttg cgctgcgcac gcatttccaa
     6601 gacttcgcgg atgagcgttg tcggtgcaac ggttacgggg tctttgacca cgccgctttc
     6661 gtggcgtttc actttggaaa cggcgcgcgc ctgcatttcg ggcggcatgt ttttatggat
     6721 gatgccgatg ccgccttctt gtgccatcga aatggcgagg cgcgcctcgg taacagtgtc
     6781 catcgcggcg gaaagcaggg ggaggttgag tgtgatttcg cgggtgagct tggtttgaag
     6841 tttaacgtct cgcggcagca cggtcgaatg tgcgggaacc aacaaaacat cgtcgaaagt
     6901 ataggctttt tctacgatac gcataatgct cggtctttca gtttgtgcaa gatgcacggc
     6961 attatagcac gttaccggcg gcttgacagt ttatcaggtt taattttggt cccctttgaa
     7021 tagctcggtt ttcctttgcc gaccactgtt gctcccgttc tttcaatttc aggaaaagct
     7081 tttttctaat ttttggtaag tggctcagtt attgaagccc tatatcgggc ggtaacttcc
     7141 agcacaaaaa aacggagtag ttctttattt attttttcct ttaattttca gtatattatc
     7201 ttaatatttc gagggtaaca tatctgctaa tctagttaca gccccatata ttatagattc
     7261 aattgaaaaa taacagattc aactgtacct ttcttatacc tgatttcttt aaagtttttc
     7321 ccattgtcaa aactataaaa aagtctctct tcctcaccag aatatcccat ttctaatact
     7381 aaacaatcta tttcacaatt accccctcca ctcatatatg tagtatcaat agaaaatata
     7441 ttgcataatt ccagagaatt atatttatct atatcaaata gttgctctcc aaaatctaaa
     7501 ccattgtttc cacctgcata atacttctct tcaaaggatg acttcaaact attaaaaact
     7561 gcatttagcg taaaatatcc atatgggcat atcttaggat atatattctc tcctattatt
     7621 atctgaattt ttccataaat aaaaccatta cattcagagt tttcttccca taaaatccca
     7681 aattttcttg ttgagtccat aataatattc atataaatcc ttatattaat aaattattta
     7741 caatatcccc cgctttcaga cggcatacgg cgtggcggcg gaatgccgtc tgaaggcggg
     7801 cgttatgata attgttccag cagcgtctgc ttcaatgagg actggatttt tgggtttttc
     7861 aggtcggggc taaaaacggt aaaactgtct tcggcgcgtt catccagtgt ggagattttg
     7921 gcatagcgca ggctgacgtt gtgggcgaaa aagacttccg ccatatcggc gagcaggaag
     7981 gggcggttga cggcggtgat ttcgacggaa taccagtcgg gatagtcttc ttcgggggtg
     8041 atggtgatgc tcggtgcgat cggcatatag cggctgcggc ggctgatgcg gcggctgcgg
     8101 ctttgggttt cggcaacggt gtgtccgtgg ataaagctgt tgagttcggc ttcgagcgcg
     8161 ctttggatgt cggggtagtc ttcgggggcg tgctgcgagg ggatttgcac gatgaaggtg
     8221 tcgaggatgt agtcgtgttc ggtgatgaag gcgcgggcgg cgaggatgtc gaagccgtgg
     8281 cggctgaaga tgcggcagag gcgggcgaac aggcgcgggc cgttgggcat gaaaaccatg
     8341 acttgaaagc tgtcgctttt gggcaggatg cggctgcgga cgatgggggt ttcaaagtcg
     8401 tggactaggt tggcggcgtg ccacaggatt tcgcgggact ggtggcgggc gaagtaggcg
     8461 gaaccgagcg cgttccatag ttttttctgc tgtttttcgg ggacggcggc gcgggtgagt
     8521 aagtcggcgg cttcctgccg gcggcggccg aagagggtgt gcgggttgcc gccgttgcct
     8581 gtaaggtagc gtccggcggc atggaagagg ctttccagca ggctggcgcg ccatgcgttc
     8641 cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca gaaggtagag cgcgctgagg
     8701 cgttcgtggg tttgcacgcg tttgcagaag gcatcgagta cgctggggtc ttggatgtct
     8761 tctttttggg cgacggcaga catcagcagg tggttttcga ccagccaggc gagcaggtcg
     8821 ctttcttctc cggtcaggaa gtggtcagcg gcaaattggc gcgcgtctgc gatgccttgt
     8881 atggcatggt cgccgccgcg tcctttggcg atgtcatgga aaaaggcggc aaggtagagg
     8941 atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt agggcagctc gtggctgtgc
     9001 atatccaagg caaggcggcg gacgttgcgg acgacggtga ggatgtggtc gtccacggga
     9061 tagatgtgga acaggtcgtg ttggagcagg ccgatgattt tttcccacgc gggcaggtag
     9121 cggcccaaca cgccgtagag gttgagaaag cgcagggtct gggtcagccc gttgccgttg
     9181 cggaaaaaac cggcgaagcg gcggcggttt tcgggatttt ggtagaagct gcggttgatt
     9241 ttgcgcgtcg cgccccacca ggcgcgcagg gtttgcggtt cgagcgcggt aatgtcgttg
     9301 cgctgctgca tgatttcgac gattttgaaa atgtgttcgg gccgtctgaa aaaaatatcg
     9361 gtgtgccgcg cggcgatttg gttgttgact tggatgtagt cgtcgtcaat ccgcagggta
     9421 acgcgcaacg gggtggagga aacgcggctt tgcagcatag gcgtgaggat gccgcccagt
     9481 tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca gttcttcgct ttggcggcgg
     9541 aggttcaagc cttcataacc catgctttcg gcgacttgcg gctgcaaatc gaacagcagg
     9601 cggtcttcgg cgcgcttggc gtttaaatgc agatggatgc ggatgtgggc gaggcggcgg
     9661 tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa tccgctgttt gagcaggtcg
     9721 ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa gcagggtgtg gatatcgcgc
     9781 agaccgcccg gacagctttt gatattcggc tccaatactg cccccgaacc ttgcgatttg
     9841 gcgtggcggt gttccatctc caccagtttt gcctcgacaa acgccgccac attgcgctgc
     9901 gcgttcattt tttccgccag tttatccacc gtttggcggt tgccaaacaa aaacctagcc
     9961 tctaaaaacg ctgtgtcccc cgtaatatca ttgcgcacgc tttcacatag ttcatcaacg
    10021 ctgccgcttt ttacagacgg catcagtttg cagtcccaca gggtttgaac aaaccgggca
    10081 atctgttcct gaatgccgtc tgaaagcggg gcaggggaga caaccgccaa atccacatcc
    10141 gaacagggat acagttcgcc gcgtccgaag ccgcctaccg ccatcaggca taacgcgctg
    10201 ttttgaaaat actctgccca caatgccgcc agtaaggttt cgactgccgc cgtgtattct
    10261 ctgaaaaata ccgacacgcg gttggctttc aaataatgcg cttcggcggc atcgcgctgc
    10321 tgtttgaagg tttccagtgc tgaagacagg tttgcaggca tttttattct ttcgattggc
    10381 gggaaaaagg gaggcggatg gttcggcggt caaataccgc tttcagacgg catttgtcgg
    10441 gtatcggcgg aattggtcat cagcttcaac cgttcctgcg gcggcagcag caactgatac
    10501 gccgccacgc ccaaagccgc cgccattttt tcgatattcg acagggcgat gttccagcgt
    10561 ttgcgctcga ctgccgacac ataagtcctg tccaaaccgc attgccgcgc caattcttct
    10621 tgcgaccaac ccttgttcac gcggaaaagc cgcatattgt atgccaatac cgcccgcaaa
    10681 tcctgttcgt caggcagttc ggcaggcaga gtcaatttgt tgcccatcat ttgcttccag
    10741 ataaatggtt aaagttaagc atttgctgtt acggatttta cttcacataa aagccaaaaa
    10801 aaaggggtgg cggcagccac acccaaacac acacacatca agaggaaaga gataaaatca
    10861 agacagattg ggaaacagca aacaagcacc ctgaatttca tatcggtatt atcaaacaaa
    10921 aacccttatc aggtattgat tcaaatcaat ttagatttta ttcgcaaacc gaaaaagaaa
    10981 aacggcatat ccgtttatac ggatatgccg tctgatggtg cgggggactg ctgtatttgg
    11041 cagtggtttg tgttttagtc ctcttgcgcc gctgtttgca ggtagttcgg cagaccgatt
    11101 ttgccgataa gctcttgctg ggtctccagc cagtctatat gttcttcgtt ggtgtctttt
    11161 tgtttttcca acaaatcgcg gctgacgtaa tcctgttgcg cttctgctgt ggcgatggcg
    11221 gcaagcaggg cttcgtgttt ttcctgttct ttggtcaaat cgcaggcgat gatttcttcg
    11281 gtggactcgc caatcagaag cttgcccagt tcttgcaggt tcggcagacc ttcgaggaac
    11341 agaatgcgct cgatcaaatc gtcggcagct ttcatttcaa cgatggactg tttgaagaaa
    11401 tgttcgccca gttcttcaaa gccccagttt ttcaaaatac gggcgtgaag gaaatattgg
    11461 ttgatggtta ccagcagcaa gcctaagttt ttgttcagct cgcggataac caaacggtcg
    11521 cctttcatac ggactccttt tattccgaat acggattaca gttggctttg gtagtagttg
    11581 ccttcgccaa ttaactcgat caggcgaagc tgctgttcca gccagtgggc gtggtcttct
    11641 tcggtatctt tcagttgggc aaccatcagg tcgcgcgtaa catagtcttg agcctcttcg
    11701 cacagtttga tgcctttttt caacgcatca cgtacttcat attcggtttg caggtcggct
    11761 ttgaggcagg aaaccacgtc cgtgccgata ttcagttcgg cgcgtgccat tttcggcgta
    11821 ccgcccagca tcaggatgcg gcggatgaag tcttcggcgt gtgtggtttc ttcttccatc
    11881 tcgtggttga gacgttcaaa aagtttggtg tagccccatt cggagtagag gcgggagtgg
    11941 ataaagtatt ggtcgcgtgc cgccagctcg ccagacagca attcgttcat ataatcaaca
    12001 acagcttgat tgccttgcat aatatctctc tttctgtaac ttggttttcg gtatgtctga
    12061 acagcatctg tgtcgctatt cgggatgcgt gcattgtatg caaaagctgt cggcatgaca
    12121 aattttctat ttaaaataca aacaattatc aaaagaaata gggcgtattc cccgatgcct
    12181 tccaaatcag tatgctgttt cttatcggtt tttttgctgc taaaactaag aatccatctc
    12241 atccataaag aacatttaca cttattaacc atagcaaaaa acaaatagag cacggttttt
    12301 tatcaaaatt tataatgaat cgttctcatt aaccgacaga ttcttttgag attatcggat
    12361 tttgggaata aattatgccg tctgaggggc tttcagacgg catgcctgcc cggacagacg
    12421 ctgcttggag cggctgtcgg tttgaaggtg tgtttaatgc gaagggcaat ctgtcgtatg
    12481 atgtgtttgc ggcaggggct ttgaagaagc ccggtatttt aggacgaagc gcgcggctgc
    12541 cggggtcaat atccgctatt tattccaaac cttattaaaa cttaaattta aaatcatgaa
    12601 gatagaaaat atcgatatta tttcgccgga actgttcccg caggaaacat tcaatgaaac
    12661 cgaagcattc ggcgctttgg tatggttgtg ggcagtttcg cctatttatc agcatgccgg
    12721 cgtacaagaa gccgccgtca atatcttgcc ggtattgaaa aacgggcaat ttgccttgtt
    12781 cagcagcaac ggacaccccg tcgcctactg cacttgggct tatttcgatg aagaaaccga
    12841 atggcaatat ctccaatcca atgatgttct gcgccactcg gaaaactggt gcagtggaaa
    12901 cagaatgtgg cttatcaact ggttcgcacc tttcggcgac agccgtatga tgaaaagaat
    12961 tctggtgcat ctgtttccga aacgtgaaat aaggtggctg taccaccggg ggagtgaaaa
    13021 aggaaaacgg attatgaggt ttccagcgtt gtcgaaacaa taaaacaagg ctgcctgaaa
    13081 gtttcccttc aggcagcctt gtttcagttc tctcgaggcc attcgtattt tttgttttcg
    13141 ggataaatgt cgtcacctac acgataccaa cctctttttc tcatgcaggc atctgcttta
    13201 cttcctcctc ccccacctat tgggtcatag ccgcactctt tccagtcttt ttttccttag
    13261 caaaaagatc atctatttgt ttgctatatt cttcataaga atatatgcgt aaattgatat
    13321 ttttactcat cattgccgga tattcttgtt ctatacggga atacttccag tataccgagt
    13381 catcgggcgg aggtttgaat cccccaaacg aacaggcagc caatcccata gccaaagaga
    13441 ttgatacgat atatttcatt ttgtttttcc ttcttctcga ggccattcgt attttttgtt
    13501 ttcgggataa gggtcgaaac ccttacgaca ccagcctttt ttcctcaggc aggcatctgc
    13561 ttcagacttt ccgccgtcta ttgggtcata gccgcactct tgcatgtctt tcagttgcct
    13621 tcgtcttgct ttcgatggat attggtcaag tgccgccgag gctgatcccg gatagggatt
    13681 ggcgtaattt ttcaattccc aaaatgacgc ggcgtcccac ggatttggtt taaatccccc
    13741 aaacgaacag gcagccaatc ccatagccag agagattgat acgatatatt tcattttgtt
    13801 tttccttctt ctcgaggcca ttcgtatttt ttgttttcgg gataaatgtc gttacctaca
    13861 cgataccaac ctctttttct catgcaggca tctgcttcac tcccaccgcc cccacctatt
    13921 gggtcatagc cgcactcttt ccagtctttt ttttctctag caaaagaatc atgcagttgt
    13981 cttcgtctct cttcaggtgg gtattggtca agtgtcgctg aggttaatcc cggataaagt
    14041 ttggcgtaat ttgtcaatct ccaaaatgcc gagtcatcgg gcggaggttt gaatccccca
    14101 aacgaacagg cagccaatcc catagccaga gagattgata cgatatattt cattttgttt
    14161 ttcctttcgg atgttgcaga tcataaatgc gtacaggacg gtacataatt tcgatttcgt
    14221 tggacactcc tctttcaaat acacctgtcg gactttgggt ttgtttattt tcagggtctc
    14281 gataaccatc tgttacacat tgtttattag ccataccgta gcaattatgc ggcgaggtgt
    14341 aatcccctat aatatccctt atagcctgcc attggctttt ttgccgggta ttggttccga
    14401 ctgttgccgg atttctgcca atcagcatgc cgataaggtc taattcgtgg ttttctattt
    14461 ggatggactg acgggcgaaa tctgccgttc tgggtgtcgt taccccctgc tgcagttgaa
    14521 acagcctttt atctgcccgg acaacgttgg cggcagggcc gaccattttc agttcggtat
    14581 tggataaaat tttcttatcc cgattggctt gggtattcaa ggtattgagt acattgtcga
    14641 ccaccagggt tccacggctg tgcgagccga caaacaggcc gtttttatcc ttcccgtagt
    14701 cttccatgat attgcctaaa gccaagcctg aattgctcaa gccgataacg gtcttattgc
    14761 ctatttttgc cccttcgagc attttatgaa aggccgcaac ggcgatttcg ggcaattttg
    14821 aaaagccccg cccattggtt tccggattgt gcaggaaata aacattttca taggtgcgtt
    14881 catatcggtt tttctcggga ttgaaacgcc ccacatattg ttgggcggca aatttggcgg
    14941 ctgcttgtat attattgaaa atgccgttga cgccgacaac gattttttca acggttttgc
    15001 cggtctccgg gtcggtgtaa cgggcagttt tcagattttt ccgttcctgg tcggatactt
    15061 cgcgcaattc gtagatattt cccccaatgg ctttataggc ttcccaataa tctttaaatg
    15121 aggcaaattc ttttttcaac tttactgagt tgtcaaactg tttatttata tcctcttcaa
    15181 gctgcttatc ttcaatcggc tcgccgtttt catccatttt aaaggtcatc aggcggtgtt
    15241 ctgcaataaa ctggctgcgg taggcttcgt ctgcaatgcc gccgatcagc gtctcattga
    15301 taaactcttt ggcaacatcc ctattgagtt cgacttcttt cagcaagcct tcgcggtctg
    15361 cttttgccaa tgccttatgt gcggatgaag tgcctttttc aataccggca atatttttcc
    15421 tgccctgcgc ggaagcaatt tgccaatctc cctcactgat gacggagtgg gtaatggatt
    15481 ggcggctttc tgattctttg gcattgccca acagattgcc caaacccatt tttgccaagg
    15541 cgtatttgtc gttgctgtgt atatcgtttt gcttcaaacc gaatttcaag ccgccggcgt
    15601 tgttttgatt taaatttgcc tctttaaagg tttccccttt ttcaatacgc tcacttcgat
    15661 atttttcgtt caactcgtcg tctttcgccg aaacctggtc aaaacgcatc aggctgaccg
    15721 gtttgccgcc gattttaccg atttctgctt cttttttggt ggaatactgt ccgctggtag
    15781 gcttcgggct gtatgaaaaa ccgccgctca agcccaaggc ggaagcagcc gccgaagcat
    15841 ggttttgaat atctttatgc cagatttcgc ttgttttcag caggtttttt gatttgtcgg
    15901 catctgaaac aacagcggcg ccgaccaatc cggttttgcc gtttacgcga atccgatagc
    15961 cgtctcctcc tgcaaagata ccgctttgct cgttgacgga tgcataatcc gagctgcttt
    16021 tcgagcggtt atagctgcca ccgacactaa agccgtagcc taccgtaact tgggcggaaa
    16081 cattttcctg tttgccttta aacacggcgg tatcctgcaa actttcgata tgcagactct
    16141 ctgccgttac gccaacgcct ttgcctttaa gctgcccgcc tttgatgacg gtatcgccac
    16201 cgctttcaat agcggtttgg ctgtctttgc tgccgatatg gctgttgcgg taggcggttt
    16261 cgtcgccgtt gccgtaacct ttgccgtagt ttgctccggc tgtgaagccg aaactgatgc
    16321 ctttgttgat ggcgatggcg actccggcat taaagcctgc ggatttgttt tcgctgcgtt
    16381 cctgatgcgt ttggcgggcg gcttcaatct gaacggcatt ttctgctttg aggcgtgttc
    16441 ctttgccgcc gtacacatcg gagccggtaa tcgtgatgcg ggagtctttg cctgcgcctg
    16501 aagcagtcag ggaaactttg ccgccgccgg tgattttgcc ctcttgcacc tgcgtgcctt
    16561 tgatgcggct ttcggaggtg ttcttctgtt cgccgtaggt aacggagaca ctgatgccct
    16621 gaccggctgc ttttttggga tttcgggcgg cattatagag tgccacgccg gaatctactc
    16681 ctttattcaa ggcgttggca gcagccatgg cattgacccg gctgtttttg cttttgccga
    16741 cggtttggac tgcttttacg gcgtcaaccg cgcccattac ggtattcaca accggaacgc
    16801 taatggcgac ggttacgcct ttttgttcgt aaacctgctt actctcttgg ctgtaacggt
    16861 tttgtgcggc atcgatgctg atttttccgg aggaaatgcc gacatcgcct tggggcgagg
    16921 atatggtcga accggtttgg gtgtaatgtt ttcctgccga aatcagggta ttgccgttca
    16981 ggctgccgac gacgctttct gtgtggctga cggtctcgga tcggttggtt tgcgtgtctt
    17041 ttttgctgcc cgccgtaaag ccgatgccgc cgctgcccat cagtccggat ttttcttttt
    17101 tgttcatttc ggcactgcgg ctgcgtgttt cggctgcttt aaggacgata ttgttttttg
    17161 ccgagagaat ggtatggttg tctgcaatga tattgctgcc agtaacggta atatcgcgtc
    17221 ctgaaaccag aatgatttct ttgccgtcca gcgtgccgga tacggcttgt ccgttttggt
    17281 tcttgagatg gcgggtcatc ttctgtttga tgccgccccc gcttctaccg gtgtatttca
    17341 gggcatcttc ggtttcggta tgggctttgc cggcttcgac tttgatatcc cgtccggctg
    17401 ccagtttcag acggccttgt tcgctgccga cctctgctgc acggatacgg atgtctcctt
    17461 ttgcattcag actgagattg ccccgggtgc ggatggtgct gccgacttcg ttttgttctt
    17521 tgcgaatcac atagttgtcg gaatcaaaga tagtgttctg attgcgggaa atgcccgtcg
    17581 tatccgagcg gatgtcgccg ccggcattca gtacggtttg accgtcttca gattgattgg
    17641 tcaattcgga agccgtcagg acgatattgt tgcctgcatc cagcaagacg cttccattct
    17701 gcctgccggt cagataaatg cctgccaccc ggccgatatt gcgtaccgag ccttgctcat
    17761 tctgattgct gcgggtctcg ctgcggcttt ctatattgtt actcgctttg agcagcaggg
    17821 cgttttctgc gccgatgctg ccggtattcg taatgtcgct gcctgctgcg gcgaagatgt
    17881 ttttgccctg caaatcacct tgcagatttt taatattctg tgcgtttaaa attagtgctt
    17941 cgcgcccggc aattaagccg ccccggtttt caatggcgcc gctgccgata tcaacaacgc
    18001 tgccggacag caacgcccct tgtccgttca tatctttggg gcgtgcgcgg acatagactt
    18061 tgggtttcaa tacggtttga gttgtcccgt cgggcagggt aacggtctcg ttttccagcc
    18121 aaacaatgtc ggaagtcaga cgggcaacct gttcggcaga cagggcaata cccggagtaa
    18181 gctgcaattc tttggctatg gtaatgccgt tatccatcaa agccttgaat tgctcttcgt
    18241 cattggtata accgtccaag cggcggtagc ctgtcagctt ggcgatttgt tcgtttacca
    18301 gtttctgctc gtaatagccg tcgcccaaac gcttgtggat atggttcggg tcttgttgca
    18361 gtgcggcaag catatagccg ctgcccagcc atttgcggta gtcggtaaag gcagggtcgg
    18421 tttcaatcaa atagcctttg ttgtttggcg caatggcaaa caagctgctg ttcggcagag
    18481 taaatgtagg atggatgccg ttttcagcaa caacgggtac aacagtgccg ggtatatcag
    18541 atgcctgttg gggcgcatag cctttatagg ctgaaatacc catacggatg gagctgactt
    18601 cgggggccgg ttcgtaggga gaccgactgt atcccgtaga atcccgccct ttcttgtgat
    18661 gacggtggta acggtgcaag tcccctttat cggttatggt ttttgttccc aaagtttcat
    18721 cattgtccaa tgcggattcg ggcgtaccga cgatcaatct gccgccggca ataatccggc
    18781 tgttatgatt tttcagcttg gcggcatcta aaaccaaatt gccgccactg atgatttgac
    18841 cgggcttgga ttcggtaatt ttgctctcgg cggtgattcg ttcgtaatcg tatttgtacc
    18901 agacagagtg ggggctgccg tccggagtcc gcatatgtaa agactcatct tcaaatattt
    18961 cccagcccaa ctctttttga gaaccttccg gatagcgttc tgttctgcct tccgcctgat
    19021 attcgatacg gtgttcgcga tgggtttctt ccgtatggaa acgcagatgc tcattggtat
    19081 tctgcaaatc ttttgtagcg atacggatat tgcctgatga ttcgattgcc gcactgcggt
    19141 tgtgcagtga tgtatttgct ccttgcacct gtcggcttcc attcaatgca gaaccgatat
    19201 gaagatcgcc ggagctggac aataatgccg cctctcggtt ctcaatttcc cgcgctccaa
    19261 tatccaaccg ctcccgcgct gcaattaccg ccgctttggt ttcgccgttg accgtttctt
    19321 cccggttcaa caaggtatct gcctcaactg ccacacggct gccgtaaatt ctgcccgtgc
    19381 cggcattgtc cgacttggct tcggtttgca gcagcgttat accgttgctg ttgattaaac
    19441 ccctgttggt gatgccgttt ttgccgttta acccggtgcg gtttccggat tggattttac
    19501 cggagacagt gttgtcgatt tgggcggcgg tcagggatac ggtatcgcct gcctgtatga
    19561 tgcgggtgtt tttcagacgg ccttgggtgg agaacgtgag tgtgcgtccg gcttcaatat
    19621 cgcgtttgcc ggcaaaatca tcatgaagag aaacggaaac atcgcgtgcg gcggttaata
    19681 tgccgtcgtt gtccagtgat tttgcctgta aggaaacatc tttgcccgca ataatcgtgc
    19741 cgtctgtgtt attgatatgc aggctgcttt tgcctgtatc gcgaatatcc agcaaaccgg
    19801 ccgagccgat taagcctgct tgattattta tgccgtctga aaccttcaat acactgcctt
    19861 tgccgctgcg gataaagcct tggcggttat caacggtttg agcggaaatg gtgatttcgg
    19921 cagcatcaat cgaaccgctg ttgctcaatt tgccgtctgc gcttaacgta acgccgcctg
    19981 ttgcggcaaa aatccgaccc ttgttgcgga ttacggcgcc gttgtcggtg ctgattaaag
    20041 tgattttgtc tgcgtacatc ccacccagtg tggcggtgtc gatggcaacg gtaggagtaa
    20101 cagaatccga agaagatggc gcagaagctg ttttggcaag agagccgtca aaatccaatt
    20161 tgttcttacc cgaaaccacc ttgacatctt taccccaaac gcccgcattg atttcagcag
    20221 cacgactaag gatacgggtg taatcggcat cagaggtatc caaacctttg cccccaatca
    20281 cgactttacc cgaagaaaca tcaaagcccg tcagattgcc gttattcaaa acaggaacgc
    20341 ccgaagtcag cgtaaccgaa gcggcattaa tcaatccgcc gccattcaca cggatgcccg
    20401 acggattggc aacgactact tcggcgcgtt tgccgccgac ttcgatataa ccgttcaaca
    20461 acgaaggatt actgctgtca atctggttca caattacccg cgcttcgccg cgtgccagat
    20521 ggggattgcc ttgaatccat ccaccgagtt gcgtttgcgt attgctgcgg ctgttgttta
    20581 gtattacgcc tttttcatca acatcgaact gcttgaatcg gttaacagaa acgccttggg
    20641 atgacggagt ttgaatattg acttgcggca aaccgtttgc tgtctgaaga ataacggctt
    20701 gttggttttt aggggcggat ttgtcggcaa tgatgccgga agcaggggca ggggaaaacg
    20761 cagcaacacc caaagccaac atgacagaaa aggcagccat acggaaaccg aaggctgccc
    20821 gggcagaaga aacagaagcg gcaccggtca ctcgaaccga agccgcctca ctatcctgca
    20881 tactcttgcc gtcacgatga acattctctg ctacagccat catacaactg cgtttcttgt
    20941 tgaagataac cttgtagcat cgcttgttca tgatgggttt tcttaaatga aatgtaagga
    21001 atagttaagg acaaaatata ggaaatttga attaaattgt caataaaaaa ctgacagcgt
    21061 accctgataa tcagatgttg tacgctaatt gtgagaagta tgtcgggatg attattttga
    21121 agttttcttt ttattcaaaa gagttactat gaaagttaac cgggcgtaaa taggcttcaa
    21181 taaacaggtt atcgttattt ttcaattggg aaaaaatctt ccctaaccca tccattcgcc
    21241 aaattattat gtgggaattc ggcaatttca ataacggctg ttgatccata tctgctcggt
    21301 catttagcgt tataaagtcg caaatagcgt cagaatttac aagattatcg gattttggga
    21361 ataaattatg ccgtctgaag ggctttcaga cggcataggc ggctaacacg ggtgcggctt
    21421 gcgccaaacg gcgcggcagg cgcagggaat cgctttttac tttggcgcgg tgttccgggt
    21481 ttaatgatgc ccgcaaccgc cgtggctttc cctcaaggct tccgccgcct gttcgtcggc
    21541 gtggtagctg gaacgtacca tcgcgccgat ggcggcattg ctgaagccca gttcgtatgc
    21601 ttctttttca aagattttga actgctcggg cgtaacgtag cgcaggacgg gcaggtgtcc
    21661 gtctgaaggc tggaggtact gtccgatggt aatcatttcg atattgtgcg cccgcatatc
    21721 gcgcataatt tcacgcacgt cttcgtctgt ttcgcccaag ccgaccatga tgccggattt
    21781 ggtcgggatg tgcggcatca tttctttata acgttttaat aagtctaaag aatgttgata
    21841 attggcaccg ggacgggctt ttctgtacag gctcggatgg gtttctaggt tgtggttcat
    21901 cacgtcgggc ggggtttcgg caaggatttt gagtgcgatg tccaagcgtc ctcggaagtc
    21961 ggggacgagg atttcgattt tggtgttcgg gctggtttcg cggatggctt tgatgcagtc
    22021 ggcgaaatgc tgtgcgccgc cgtcgcgcag gtcgtcgcgg tcgacggagg tgatgacgac
    22081 gtaacgcagg ttcatggctt tgacggattc ggcgaggttt ctcggttcgt cggggtcgag
    22141 catattgggc cgaccgtgtc ccacgtcgca gaacgggcag cggcgggtgc agatgtcacc
    22201 cataatcatg aaggtcgccg tgcctttgct gaagcattcg ccgatgttgg ggcaggaggc
    22261 ttcctcgcaa acggtgtgca tcttttgttc gcgcaaaatg tctttgattt caaagaattt
    22321 gcgcgatggg agtttggcgc gtatccattc gggctttttc agtttttcct gaagggggac
    22381 gactttgatg gggatgcgcg cggttttgtc cgcgcctctg agtttgatgc cgcgtttggg
    22441 gtcgtcggtt ttgatttcac tcattgttgt ctgctttcgg tgtgaattgt gtttcaaggt
    22501 gtgcggtgag tttggcggcg acttcgtccg gcgtggggca gggttggaca aaatccgcga
    22561 tttgcgtcat ttccataccg gcgtagccgc aggggttgat gtgggtaaac gggcttaaat
    22621 ccatattgac gttgagcgca agcccgtgat agacggagcc gtttttgata cgcagcccca
    22681 gtgaggcgat tttgcgttct ccgacataaa cgccggggcg tttggggtct gccgccgctt
    22741 cgatgccgta ttctgccaat gtggcgatga tgctgttttc aagcgcggaa acgatgtttc
    22801 taacactggt tttgcgccgt ttgaaatcaa tcatcgtata aacgaccaat tgcccgggcc
    22861 cgtgataggt aatctgcccg ccccggtcga tttggacgac gggaatgtcg tcgcgaatca
    22921 gcaggtgttc gggttttccc gccagtcctt gtgtgaacac gggcgggtgt tcgacgaccc
    22981 acagttcgtc ttcggtgtcg gcattccgtc cggcattaaa ggttttcatc gcttcaaaag
    23041 tcggcagata ttcgaccaaa cctttgtgta tgattttcat ctcaaagtac cactttgacc
    23101 agttcgtgcg aagtcagcgc acggtagatg ttgtccaatt gttcttggtt ttcaaccttt
    23161 acctgtacgg tggcgccagt atagttgcct ttgctgctcg gacgcgtggt gatgtggtgc
    23221 gcctgcgtgt cgggggcgtg gaggcggacg gtgtctaaaa ccgcctgctc gaactcggga
    23281 tgcaccgcgc ccattacttt caatgggaag gtgcagggaa attcgatgag ggatgttttg
    23341 tttttttgtt cggtcatgat gtgctgcctt gtcgtgtacg gtatgccgtc tgaaggcggg
    23401 tttgcctttc agacggcatc ggatgtgcgt tattttagcc taaaccgcga taacaggcta
    23461 tcgggaaggc ggaggctttt ttgacggcgc ggcggttctg ctatactggc gcacaatatt
    23521 atttttagaa gggtggtttt tatgtatcgg aggaaagggc ggggcatcaa gccgtggatg
    23581 ggtgccggtg cggcgtttgc cgccttggtc tggctggttt tcgcgctcgg cgatactttg
    23641 actccgtttg cggttgcggc ggtgctggcg tatgtattgg accctttggt cgaatggttg
    23701 cagaaaaagg gtttgaaccg tgcatccgct tcgatgtctg tgatggtgtt ttccttgatt
    23761 ttgttgttgg cattattgtt gattatcgtc cctatgctgg tcgggcagtt caacaatttg
    23821 gcatcgcgcc tgccccaatt aatcggtttt atgcagaaca cgctgctgcc gtggttgaaa
    23881 aatacaatcg gcggatatgt ggaaatcgat caggcatcta ttattgcgtg gcttcaggcg
    23941 catacgggag agttgagcaa cgcgcttaag gcgtggtttc ccgttttgat gaggcagggc
    24001 ggcaatattg tcagcagtat cggcaacctg ctgctgcttc ccttgctgct ttactatttc
    24061 ctgctggatt ggcagcggtg gtcgtgcggc attgccaaac tggttccgag gcgttttgcc
    24121 ggtgcttata cgcgcattac aggcaatttg aacgaggtat tgggcgaatt tttgcgcggg
    24181 cagcttctgg taatgctgat tatgggcttg gtttacggtt tgggattggt gctggtcggg
    24241 ctggattcgg ggtttgccat cggtatgctt gccggtattt tggtgtttgt cccttatctc
    24301 ggggcgttta cgggattgct gcttgccacc gtcgccgcct tgctccagtt cggttcgtgg
    24361 aacggcatcc tatcggtttg ggcggttttt gccgtaggac agtttctcga aagttttttc
    24421 attacgccga aaatcgtggg agaccgtatc gggctgtcgc cgttttgggt tatcttttcg
    24481 ctgatggcgt tcgggcagct gatgggcttt gtcggaatgt tggcgggatt gcctttggcc
    24541 gccgtaacct tggtcttgct tcgcgagggc gtgcagaaat attttgccgg cagtttttac
    24601 cggggcaggt aggcggttcc gaaacatata gtggattaac aaaaatcagg acaaggcgac
    24661 gaagccgcag acagtacaaa tagggcaacg ccgtactggt ttttgttaat ccactatatt
    24721 tgaagcggaa tacaaccttg cccgggttta aataaaaatg ccgtctgaac cccgaaaact
    24781 ggacttcaga cggcattttc atcacggctt atttggcggt tttgctgctg tcgataattt
    24841 tcataccggc agaaatcagg ctgccgatgt cggcaacatt ggcgggcata atcagcgtat
    24901 tgctttcttt ggcaagattg ttgaacgcag cgacgtattg ttccgcaatc ttcagattga
    24961 ccgcatccgc accgccttgg gtttgaaggg cggcggcaat ttgacggatg gcttcggcat
    25021 tggcttcggc aacaaggcgc aaggattccg cttcaccttt ggcgcggttg atgcgggcga
    25081 ttttctcggc atttgacgca ttgaccgcag cctgagcctc gccttcggat tgttggattt
    25141 cggcttcgcg ctgaccactg gcaaggttga tttgttcgat tttacgacct tcggattcgg
    25201 cgatacgggc gcgtttttcg cgttcggcag taatttgcgc ctgcattgag cgaaggattt
    25261 cttgcggcgg aaccaagtct ttaatctcat aacgcaaaac cttcacaccc caagccccgg
    25321 ccgcctcgtc caaagccgca acaacagtac tgttgatttc gtcgcgttct tcaaacgttt
    25381 tgtccaactc catacgcccg ataacggaac gcagcgtcgt ttgggcaagc tgggtaatcg
    25441 ccataatgta gttgctcgaa ccgtatgagg cgagtttggg gtcggttact tggaaataga
    25501 tgatgccgtc aacagtcagc tgcgtattgt cgcgcgtgat gcagacctgg ctgggtacgt
    25561 ctaaagggat ttctttcagc gaatggcggt aggcgacgcg gtcgataaag ggaatcaaaa
    25621 tattcaaacc ggccgtcagg gcgcgatgga aacgccccag cctttcgaca acgtggactt
    25681 cctgttgtgg gatgacaaca aaggatttga aaccgaaaac ggcgacggct accaacaaga
    25741 taatgaaaaa ttccataatt cctccgagtg ttaagggtgt gtgataataa gaaggttgcc
    25801 ttccttgcgg acaatgaggg cgcgagttcc tggttcaagc tcttcttgcc ccgtattttg
    25861 agcctgccag tgcgtaccgc gataaaaaac ttcgtaacgg ttgccgcctg tgtgtcggag
    25921 gatttcgaca tattgtccgg catccaaatc ctgatatgaa tccgtttcaa cttttctaac
    25981 ggcggttttg gcgtgtacga accaaatacc cagcgcggaa agcagagcgg cggtcaagac
    26041 ggcggcaggc gtactgccgg tcagcccgta agcaatgccc gaacccgcca aagccgcgct
    26101 gacaaccaaa agataaaccg ttcccgtcaa taattcgatg attaagacgg caacagcggc
    26161 aacaaaccat acagtcatac atttccccac aaagcgcgtc gtttgacaaa ataacgcaat
    26221 atcagcagta tagccgaatt tgaaaggata gggcagatat ggacacttgg cacgatgcac
    26281 tcggcggcga aaaacagcag ccgtattttc aggaaatttt aaatgcagtc aggcaggaac
    26341 gtttgtcggg acaaatcatc tatccgccgg cggcggatgt gttcaacgca ttccgcctga
    26401 cagcgttcga ccgggtcaaa gccgtcattc tcggacaaga tccgtatcac ggggcagggc
    26461 aggcgcacgg tttggcattt tccgtccggc agggtatccg cataccgccg tctttactca
    26521 atatctacaa ggagttggaa accgacatcg aaggcttttc cattcccgcg cacggctgtc
    26581 tgacagcgtg ggcggagcag ggcgtattgc ttctgaacac ggttttgacg gtgcgtgcag
    26641 gacaggcgca ttcgcacgcc cttttaggct gggaacgctt taccgatacc gttatcaggc
    26701 agcttgcgac acaccgcaag caccttgtct tcatgttgtg gggtgggtat gcacaacaaa
    26761 aagggaggct gatagacagt caaaatcatt tgatattgac cgcaccgcat ccgtctcctc
    26821 tgtcggcata tcgcggtttt ttcggctgcc gccatttttc acaggcaaac agctatttga
    26881 gccggcacgg tatcgatccg ataaactgga agctgtgaat gccgatatag ccgttgccgc
    26941 cggcgtgtta aaatcgcgtt tgatttgtaa tttccattta ttaggcaaaa ccttatggca
    27001 atcaaaaaaa aatcagcttt atgcatcact ttgggcgggc tgcgacgagt tgcgcggcgg
    27061 tatggatgcg agcctttata aagaactatg tgcttacgct attgttttta aaatatgttt
    27121 ctgataagca taagtacggc ggcggcatga ttgagctgca cgccggtacg acttttgacg
    27181 acatcgtcaa actcaaaaac accgccgaca tcggcgaccg cctgaataag attatcgccc
    27241 aaattgccga agccaacgac ttaaaaggcg tgatcgacgt taccgacttc aacgacgaag
    27301 acaaactggg taaaggtaag gagatgatcg accgtttgag caggcttgtc ggcattttta
    27361 aaaagctcaa cctttcttcc aaccaagccg aagacgacga tttgttaggt gatgcctacg
    27421 aatacctgat gcgccatttt gcgaccgagt caggcaaatc caaagggcag ttttacacgc
    27481 ctgccgaagt ctcccgcatt atggcgaaga ttatcggaat cagcgcagat tgccgtccag
    27541 caccagcgtt tatgacccga cctgcggctc gggttcgctg ttgctcaaag ccgccgccca
    27601 agccggcagc caaatcagcc tttacggtca ggaaaaagat gtggcaaccg cgtcccttgc
    27661 ccgtatgaat atgattttgc acaacaacga aaccgccgaa atcaacaccg ggaacacctt
    27721 gtccgattcg tctttccgtg atgaaaacga cgggcttaag accttcgatt ttgccgttgc
    27781 caatccgcct tatcccgccc gaaaaaaacg gcgattacgc ctttttgctg catctgctca
    27841 aaagcctgaa accaagcggc aaaggtgcga ttattcttcc gcacggtgtg ctgtttcgcg
    27901 gcaatgccga agcgcgtatt cgcacggaat tgcttaacct tgaccttatt aaaggcatta
    27961 tcgggctgcc tgccaacctg ttttacggca cgggcattcc tgcctgcatc atcgtcatcg
    28021 acaaagaaca cgcccaaacc gcccaatttg ccgaagaggg aacaaaccaa gttatcagcg
    28081 gcggcagcgt gtttatgatt gacgcatcgc gcggcttcat taaagacggc aacaaaaacc
    28141 gtctgcgtga gcaagacatt cacaaaatca tcgacacttt cacaaacctc gttacagccg
    28201 tatggtgcat ttaagcgaaa tcgcagcaca agattacaac cttaatctgc ctcgctatat
    28261 cgacagcgga gaagtcgaag acctgcaaaa tctcgccgcc tatctatctt tatggcggca
    28321 tacctgcgca cgatatggac gcattggaag cctattggca agttttaggc cgtatgaaaa
    28381 acgagttgtt tgccgaacac gatggccact ttaccactat acaacggaat cgattgcaaa
    28441 tctttcccac tctcaacagc ttaaaatcct gcgggattgg tgtggaattt agggctaatc
    28501 tagtacagcc ccaaatttaa tccactataa aatcgaaagc agccaaatca aagcccatat
    28561 attggcgcac cccgattacg ccgccttcaa agccggacac ctagcaaagt ttgccgcgtg
    28621 gcacactcaa aacgaccttg ccgccatcca accgggcagg cttatccgga aatggagcga
    28681 aagcctgctg gacgcgttca aacccggcag cctgattgaa gaatacgatt tctaccaaat
    28741 cctgacggac tactgggcgg aaaccctgca agacgatgtt tatctcatcg cccaagatgg
    28801 ctggaaggcg gttaaaaacc tggccgaaat caccaaagaa agcgatgaag ccgcgaacct
    28861 gaccgtcgtc tttgaggaaa ccgaaaccga caaaaaaggc aaagccaaaa ccaagcgcat
    28921 cagcaaaaaa taccgcagcg aagtcatcgc ccccgagctg gttgcccgcc gctacttttc
    28981 agacggcatc gccaagctgg aagaaaaaca aagcgagctg gaacgcctaa gccaagaatt
    29041 ggaaaaccac atagaagaac acggcggcga agagggtgcg ctgaacgacg tattggatgc
    29101 aaaaggcaaa ctttccgcca aacttctgaa aaccgcattg gaagaaagcg gcatagaaga
    29161 aggcgaacgg gctgttttac aaaccaccca aacactgatg acgcaggaaa aagccgcgaa
    29221 agacgcagtc aaaacccaaa tcgaagccct gaaccttgcc gtattcaaac aatttggccg
    29281 actttccgaa gccgaaatca agcagcttgc cgttcaagac aaatggcttg ccgatttaca
    29341 aagccgaatc gaaaatcgct tggaaaacag tattcagcag cttatcagcc gcttgaacac
    29401 gctggaagac cgctaccgca gcccgatggc cgagcttgcc cgagaagtgg aaaagtggca
    29461 aagcaaagtc aatgcccacc ttgaaaatat gggttttgga ggctgaaatg gcagcacaga
    29521 caggctataa ggcgagcggg ttttgagacc tttgcaaaat tccccaaaat cccctaaatt
    29581 cccaccaaga catttagggg atcgcggttc gggtgtccgc accgcttaat acgtcgtcgt
    29641 ccacgaactg acccatttgc tcgaacgcca tcgcaacgcc cgttttatgg cgcatatgga
    29701 caactttctc ccaaactggc aaagcatcaa acaacagctt aatgccttgg agttatttgc
    29761 acaaatatat aatttaacat aatatacatt atgcgaacta tcggaaacag ttgtacgtgt
    29821 ccctgtggtc tttccaagta ggaaaattaa agtatggcca atgcggctga atgtatagcc
    29881 cggagcatcc gcgtatccga aggtactgaa cgacatgccc tgcatccctt tcgacgcttc
    29941 catccagccg gtattgggaa atacgttgac cttcaatata atatacaggc agccgacaca
    30001 caggatatgc cgctgctttt tcatcatttc ttctgtcaaa tccttggaac ggtcgatttg
    30061 aaaacacggc tttacatacg ccccagttcc ctttgcaggg cttgaatatt ttgctgcctg
    30121 tccaatacat tgctttgtaa tgcatttatt tcttgatggt tgatgttgcc gccctttgcc
    30181 agacgtgctt gtgataacat tttttgggct tcaaccaatg ctttgcgttc gttgctcaat
    30241 tctgtttcga gaatggagcg tctgctgttg tttgagggtg cttgttgcgg cggcggcgta
    30301 ttggattttg ccggcttgga tactgttttg accggggctt tatatttgac aacctgtccg
    30361 ccgtttgacg gtgatgatac cggttcgggc gtttggggcg ggatatagcg ttcgctgctg
    30421 tagttgccga ttgggggcaa atcggttgag tggcagcttt tggacggctt ggtggtgtaa
    30481 acggtttctc cgttgattgt gcaggtgtag attttggccg cattcgcacc caatgggctt
    30541 gaaatcaggg aaaagttgat taggattaag aggagttttg atttcataat gtgcttgatt
    30601 ttcggataat catttgattt tttggtattt tttgtaatgc tttcttggcg ttttacgggc
    30661 ttttcttttc ggttggttgc ttttgatttt ctttcggggc tttggcagcc tgcttgttct
    30721 ttttcccggc aggttttttg tttttctttt tactttctat gtgtattttg gcttcttaac
    30781 tgagtttttt aattttcagg cggtatccgc ctccctgatg gctgctgatt ttaggtaaat
    30841 ccgcatcggc gcacaatcct gctggggctg atgtatatac gcttttgcca tttgagttgc
    30901 aatggtatac ggaggcttgc gccgccgcgc cggaaaggga caggagaccc aaggcggcaa
    30961 aaagtctgat gtttataccg gtaaaaggcg gtgataacgc ccgaattata ccgttattgg
    31021 caggcaaaga taagcaccct gcccgcgctt ctttatcgct cggcaaactg tttctcgatt
    31081 tcagtttcaa tctgttccaa ttcttcttgg caagccagcc aaacctcttc gatttgggca
    31141 agttgtgttt tgacttttgc cagctcggat aaggtgtcct gcaatttttc tttgttttcc
    31201 tcgaagtaag cttcttcttg tgctaaaaat gcttcacatg ccgtctgaat ttcggaaagc
    31261 tgcgccattt ctttttcggc acggtctatt ttctgctgta tcggcttgcc gcgtcgggct
    31321 ttttcctgac ggatttgcgc ttcgatgcgc ttggtgtctt tgcggctttg gctttgtgcg
    31381 gatgctgcgg gcgcgacggc ggcgttttcc tgtgccaaac gccattggcg gtagtcgttc
    31441 aaatcgccgt cgaagttctt cagacggcct ttatcgatca ggaggaagct gtcggtcgtg
    31501 gcttcaagca ggctgcgatc gtgcgatacg acgattaagg cgccttggaa actttgcaga
    31561 gcgagcgtca aggcgtggcg catatccaaa tccaaatggt tggtcggctc gtcaagcagc
    31621 agcaggttcg gcttttgcca gataatcatg gcaagagcga gtcgggcttt ttctccgccg
    31681 gaaaatggtt cggttttctg caacgccata tcgccgacaa aattgaagcc tccgaggaaa
    31741 tttcggattt cttgttcgcg tacttcggga gaaagctgct gaatatgcca aacagggttt
    31801 tggtcggagc ggatggtatc gagttggtgt tgggcaaaat agccgatatt gagtttttcg
    31861 gaacggacga tgctgccgga gagtaaatcg attgtgcctg ccaaagcttt gataaaggta
    31921 gatttaccgc tgccgttgac acccaataaa ccatagcgcg cgccgctttc cagcgacagg
    31981 gtaatgtcgt gcaaaacagt tttgccttcg taacccaaat ctgcgtgttc tagctttaac
    32041 aaaggattgg gcagatggtc gggatggtaa aactcaaagg aaaactcgct gtccagatgc
    32101 gcgggagcga tgcgttcgag cttcgccaaa gccttcatgc ggctttgcgc ttgaacggct
    32161 ttggtggctt tggctttgaa gcggtcgata aaggattgca aatgtttgat ttgcgcctgc
    32221 tgtttgacat aggcagcttg ttgttgcgcg agacgctgcg cacgttcgtt ttggtaaaaa
    32281 tcgtaattgc cgccgtattg cgtgagtttt tgctgcgata attcaatggt ttgggtagtt
    32341 tccgcgttga gaaaatcgcg gtcatgggaa atgatgattt gcgtgcaggg taaagaagca
    32401 aggtggtttt ccagccacaa gacggtttcc aaatccaagt ggttggtcgg ttcgtcaagc
    32461 aagagcaaat cggcgcggca aatcagggct tgcgcaagat tcaggcgcat acgccagccg
    32521 ccggaaaagg atttgacggg gcggctgtgt tcttcttgcg aaaaacccag cccgttcaac
    32581 aattttgccg cacgcgccgg cgcggtataa gcgtcgattt cttccaattt agcatgatat
    32641 tccgcctgct tcatgccgtc attttgcgct tctgcctgcc tcaatgccgt ctgaaaagcc
    32701 tgcaactcgg catcgccctg caaaacgtaa tccaaagcgg aaatatccaa atcgggcgtt
    32761 tcttgggaaa cggaagcgag ccgccagttt ttcggaatcg agacatcgcc gccgtcctga
    32821 gtgatttcac ccttgattaa ggcaaacagg ctcgatttgc ccgttccgtt tttgccgatc
    32881 aaaccgacgc gctgaccggg attgacggta gcgttggctt tgtcgagcag gactttcaaa
    32941 ccgcgttgca gggtgaggtt tttgatttca atcataacgg aaacatcgtc gggcgggaaa
    33001 agcccgtatt ttacctgaaa gtcagtgccg atgccgtctg aaacgggaaa tttacggctg
    33061 aagccaagcc caagccctgc gcccttccga gtgcaggaaa accaatgtcc tgaatgccga
    33121 atcggtattc atgcattcca cgctgattcc gattcgggaa aaatccgcca tgattttggg
    33181 atggataaac tcctgagccg cgcccgtccc gataatcaat atttccggat agtcaacagg
    33241 tttgacgtcg gacaacaggt tttccggagt cagatcggac aaggttcggc attgcgacag
    33301 gcagaccgaa tccttatgta caagcacggg tttatggaaa ctttgccccg ccagccggat
    33361 tccgcccgca ccgcattcat attccgcaaa ctgtccgtct atcggatttt cttcaaacaa
    33421 cattttttta ccccgttgcc gcatcatcta caccgaaagg gatgcaaaat cagacaaatt
    33481 catgtaggat tggcagattt catctgaccc gcctgccgat ttcagacggc atttgattca
    33541 aagtgcggca caattatatc ggcagcggat attttcgtct ttcaatattt acatttcagt
    33601 cggcttacaa ggagacacaa tgaagccagt aaacatcggt cttttaggtt tgggtacggt
    33661 cggcggcggt acggctgccg tgttgcggga caacgcggag gaaatttccc gtcgcttggg
    33721 gcgcgaaatc cgtatttctg ccgtgtgcga tttgagtgaa gaaaaagccc gacaaacctg
    33781 cccgtccgca gcctttgtca aagatccgtt cgaactggtc gcacgtgaag acgtcgatgt
    33841 cgtcgtcgaa ttgttcggcg gtaccggcat tgccaaagat gcggtgttga aagccattga
    33901 aaacggcaaa cacatcgtta ccgccaacaa aaaactgctc gccgaatacg gcaacgaaat
    33961 cttcccgctg gcggaaaaac aaaacgtcat cgtccaattt gaagcggcag tagcgggcgg
    34021 tatcccaatc atcaaagccc tgcgcgaagg tttggcggca aacaggatta aatccatcgc
    34081 cggcattatt aacggcacca gcaacttcat cctctccgaa atgcgcgaaa aaggcagcgc
    34141 gtttgccgat gtactgaaag aagcgcaggc attgggttat gccgaagccg atccgacctt
    34201 cgacatcgaa ggcaacgatg cgggccataa aatcaccatc atgagcgcac tggcattcgg
    34261 cacgccgatg aacttttccg cctgctacct cgaaggcatc agcaaactcg acagccgcga
    34321 catcaaatac gccgaagaac ttggctatcg catcaaactg ttgggcatta cccgcaaaac
    34381 cggcaaaggc atcgagctgc gcgtccaccc taccctgatt cccgaaagcc gcctcttggc
    34441 aaacgtcaac ggcgtgatga acgccgtgcg cgtcaacgcc gatatggttg gcgaaacctt
    34501 atattacggc gcgggcgcgg gcgcattgcc gaccgcttcc gccgtggttg ccgatatcat
    34561 cgacatcgcc cgcctggttg aagccgatac cgcccaccgc gtaccgcatc tggcgttcca
    34621 acccgcgcaa gtccaagcgc aaaccatcct gcctatggac gaaattacca gcagctacta
    34681 cctgcgcgtc caagccaaag acgaaccggg cacgctgggg caaatcgccg cgctgttggc
    34741 acaagaaaac gtgtccatcg aagcactgat tcaaaaaggc gtgattgatc agaccactgc
    34801 cgaaatcgtg attctgaccc acagcacggt cgaaaaacac atcaagtcgg caatcgcagc
    34861 catcgaagca ctggattgcg tggaaaaacc gattaccatg atccgcatgg aaagcctgca
    34921 tgactgagcc gaaacacgaa atgctgacga aagagcaggt tgccgcgcgc aaaaaagcaa
    34981 aagccaaaat ccgcaccatc cgcatttggg cgtgggtcat tttggcgttg ctcgctttaa
    35041 ccgccctgct ctcccaatgc gcgatgtcca aaccgcaggc aaaacagaaa attgtcgagt
    35101 cttgcgtgaa gaatattccg tttgccgaaa aatggcaaaa cgatttgcgg gcccgcggtt
    35161 tagattcaaa caatacccgc ctcgccgtcg actactgcaa atgtatgtgg gagcagcctt
    35221 tggacagatt gagcgagaaa cagattagat ccttcggcaa actcggcgca caagaacagc
    35281 ttgacctgct cggcggcgca aatgcctttg aagcacgtga caagcagtgt gttgccgatt
    35341 tgaaatcaga ataatgtgga ccgataaaaa agccgattct ttaaagaatc ggcttttttc
    35401 ataaaaaacg gcttacagtg cgtctttcaa agctttgccg gcgcggaatt taggcgtttt
    35461 ggcggcggca atggtcagag gctcgccggt tttggggttg cggccttggc gttccgcacg
    35521 ttcgcccacg tagaaagtac cgaaaccgac caaagtaacg gtgtcgcctt gtttcagggc
    35581 ggtggttact gcattggtag tggcatccaa agctttttgt gcggcggctt tggaaatgtc
    35641 ggcttcttga gcaatcgctt cgatcaattc agacttgttc acaatcagtc ccttcctgtc
    35701 ttaaaaaatg atgaaatgcc cgaatactcg gggtttgtac tgcttgagca actttcgctt
    35761 tatagcaatt ctgaaattgc cgtgtcaagc aaaaaatacg gaatcaccct atttgacagg
    35821 ctttcaggac gaaaccgcat ttttacaaca catttcctgc gtttcaatgt ttggttgccc
    35881 tgctgcgggg ttttggtttt gaagcggatt ccgccgccgc ttccgcacca gaaggttctg
    35941 cccaaggctc aggctggctt tccaaaccca gagccaatac ctcgtctatc catttgaccg
    36001 gatggatggt caggccggtt ttcacgtttt cagggatttc ttccaagtct ttgacgttgt
    36061 ctttcggaat caggacgtgt ttgatgccgc cgcgcaaggc ggccaacagt ttttccttca
    36121 aaccgccgat gggcaaaact tcgccgcgca gggtaatttc gcccgtcatc gccacatcgg
    36181 cgcgtaccgg gattttggta aaggcagata ccgccgccaa ggtcatcgca atacccgcac
    36241 tagggccgtc tttcggcgtc gcgccttcgg gaacgtggat gtggatgtct tttttctcgt
    36301 aaaaatcagg agccaaaccc actgattccg cacgggagcg gacaaccgac cacgctgcgg
    36361 acacggattc cttcatcaca tcgcccaact ggccggtgca ctgaatcacg cccttacccg
    36421 gcaatgctgc ggcttcgacg gtcagcaatt cgccgccgac ttccgtccac gccaaaccgg
    36481 taacctgccc gatacggttt tcgctttcgg caacgccgta atcgaagcgg cgcacaccca
    36541 aatagtcgtg cagatttttc tcatttactt taaccgcttt aggtttggct ttgctggttt
    36601 tcttggtttc agacaacctc ttcttatctt cgtccaaggt aatctgcatc accaccttgc
    36661 ggcagatttt ggcaatttcg cggtcgagcg aacgcacgcc cgcctctcgg gtgtaataac
    36721 ggataatatc gcgcaccgcg ctttcttcga ttgccaattc cccttctttt acaccgttgc
    36781 gcttcatttg cttcggtacg aggtactgca tcgcgatatt gattttttcg tcttcggtat
    36841 agccggacag acggatgatt tccatacggt cgagcaacgg agtcggaata ttcagactat
    36901 tggatgtggc gataaacatc acatcactca aatcgtaatc cacttccgca taatgatcgg
    36961 caaacttgtt gttttgttcg ggatcgagca cttcgagcaa cgcgctggcg ggatcgcctc
    37021 ggaagtcgtt acccaatttg tcgatttcgt cgagcaggaa caaggggttt ttcacgccgg
    37081 cttttgccat attctgcaaa atcttaccgg gcatagagcc gatataggtg cggcggtgtc
    37141 ccctgatttc gctttcgtcg cgcacgccgc ccaaagccat gcggacatat ttccgccccg
    37201 ttgctttggc gatggattcg cccaaagagg ttttgcccac gcccggaggg ccgaccaggc
    37261 acagaatcgg gcctttgagt ttgtccatac gtttttggac ggcgaggtat tccaaaatcc
    37321 gttctttgac tttttccagg ccgtagtggt cggcatccag caccagtccg gctttggcga
    37381 tgtctttgct gacgcgggat tttttcttcc acggcagctc gagcaaagtg tcgatgtagt
    37441 tgcgtacgac ggtggattcc gcagacatcg gtggcatcat tttgagcttt ttcagttcgg
    37501 acaggcattt ttcttccgct tctttggtca tacccgcctt tttgatatct gcttccaagg
    37561 catccagttc gccgttttcg tcttcttcgc ccagttcttt gtgtatcgct ttaatctgtt
    37621 cgttcagata atattcgcgc tgggattttt ccatttggcg tttgacgcgt ccgcgtatgc
    37681 gtttttcggc ctgcataatg tcgagttcgg attccagctg tgccagcagg aattccatcc
    37741 gtttgccgat ttcgggaatt tccaaaatct gttggcgttg cgccagtttc aactgcaaat
    37801 gcgctgcgac cgtatcggtt agccggctgt tttcggcaat gccgttgatg ctgccgataa
    37861 tttcggcggg gattttttta ttgagtttgg cgtattgttc aaactgcgcc aacagggtgc
    37921 ggcgcacggc ttcgaggtcg gtattgccgc ccgtgtcttc ttccacgacc gtctctatat
    37981 gggaaacgaa cagaccgccc gtgtcttcaa tggtcagaac acgtccgcga tacagccctt
    38041 cgaccaatac ttttaccgtg ccgtcgggta gtttcaacac ttgcaggact tgtgcgaccg
    38101 taccggtctg atacaggtcg gcggcaatcg gttcttctac cgccgcatcg gtttgcgcca
    38161 acaggaaaac cggctcctcg cgggtaatgg cgttttccag tgcggcgatg gatttcggtc
    38221 tgccgacaaa cagcggcaga accatatgcg ggtaaacgac gacatcccgc aaaggaaggg
    38281 ttgccaaggc ggcatattcc tcaaaatgct tttctttttg tgtcataggt actctcttgt
    38341 gtctgacaga ttgccgattt tcgcgtacat tggggttgaa ggtattattt caagcatatg
    38401 tggtttattt atggagtttg atgcgatgcc gtctgaaaca ttccggcttc agacggcatg
    38461 ggcttggaaa gacaaggcgg gaacaaaaaa ctgttctgtg ttgccgctcc ttgctgtacc
    38521 atccgtatgg tttgcggttc tgccgcccta ttttcaaaac atgacgcagg tatcccatgt
    38581 cttcttttat ttttcccgat gacgggttcg aatggcgcaa cgaaagtctg caaaagccgc
    38641 ccaaaggttg gcattatgtc cgcgaaagcg gtgcagacgg cattttgaag gctgcctatc
    38701 aaaatattgc aactgtctag cagggcgatt tccacaatgc caaacaggtg ctttctgcaa
    38761 tgaagaagag ggttcgcaaa cctgccgccg tccgttccga tgcggatatc gccgcccttt
    38821 tccatgccca ccgtatgaag caggcgcagc agagccgtat tctgaatatg cttgccgttg
    38881 aaatccgccc cggttttgtg ttggacaaca aacgcgcgcc cgatatacgc tccgctttgc
    38941 tcgacgtgta cggagaggcg gacggcaaac cgtttttcct gccgctcaat ctgctgctgg
    39001 ggtttatggg tgcgcacgag tggcataaga aaggggttgc cgttccgcag ctgggcggca
    39061 gcatacacgt tcctttcggc gtattctcgc cgttgcgcgg cgaatacctc gacctgctcg
    39121 cccatgcgcc gtcaacgggt tttcagacgg cattcgatat cgggacaggc tccggcgtgc
    39181 ttgccgccat tttggcgaaa cagggcattc cttccgtcat cggcacggat accaatccga
    39241 aagccgtcgc ctgcgcccgt gccaatattg cccgtttggg ctttgaaaaa caggttgaga
    39301 tacgggaaac cgatctgttt cccgaagggt ttgccgatct gattgtctgc aatccgccct
    39361 ggcttcccgc caagccgact tccgccgtcg aatccgcgtt atacgacccc gaatctgcga
    39421 tgctggctgc gtttttgcgg gatgcgccga aacatctgaa tcccgacgga gaaatccgcc
    39481 tgatcatttc cgatcttgcc gaacatctgc acctgcgtcc atccgatttt ctggataagg
    39541 catttgctca ggcgggtttg cgtgttgccg atatgatgaa aaccaagccg aagcacaaaa
    39601 aagccgcgaa tccgagcgat ccgcttgctt ttgcgcgaac ccgggaaacc actttcctat
    39661 accgtttgaa aaaggcataa ggggcggcgg cgcagcattc gggcggatta ttcttgtgaa
    39721 aatacccgct cgagcatact gcccaatgcc gtctgacgcg ttttgacggt ggcggcagct
    39781 tgccggaagg cttcttcccc gccgaagaat acgaacttct ctctcgcgcg ggtaatggcg
    39841 gtatataaca gctccttact caatccggac aatgcatcgt ccccttcgtc cgaaggtgcg
    39901 gcggaaggcg gcagcagcca tacttcccgg tattccgaac cttggctttt gtggacggtc
    39961 atggcgaatg cgggttcaaa ttcgggcagc cagcttaccg ctaccttttt aaatccgtcc
    40021 gcatcggcaa aataggcggc aaggctgccc tgccgtccga catcttccat aatcagtccg
    40081 atgtcgccgt tgaacagttc aagcgcgtag tcgttctgcc tgatcataat cggctctccg
    40141 gcaaaatatg ccaaatgttc cggtatgttc attttgcggc gtacatggcg gcaataggct
    40201 tcgttgaagt cttccgcatc ctgccgccaa gctgccagaa ccacgatatc cgaaatgccc
    40261 gcgtatgcgg cttcgatatt gccgtctttt accgcctgcc aataggcttt gtgtgcccgg
    40321 tacaaccttt cgactcgagc gttcggactg cattccgaat gttccagttc gtccggaaac
    40381 cggtcaaaca atgcccacgc cccttcatcg cccgatacgg cggcacgggc aaggcagccg
    40441 atgccgctgt tgtcgccgaa gcggtggctg aacgacagat gggcggtgtt ttgcgccaac
    40501 acgggtggat ttgcgctgac gctgaaaccg tgttccggaa ggaagccggc cagcctttgg
    40561 tgcgtttctc cgtccaaaac ggttttttgt gacaaaacgg acagcaccgc ccctattccg
    40621 acggacggga gctggttttc atcccccagc agaatcacgc gcgcgccggt tttgaccgct
    40681 tttaaaagtt gcagcatcaa tgccgtatcc agcatagagg cttcatcgat aaccaatacg
    40741 tcaaacggca gcgggtaaac agggttgaac gccgcctgca ttttgggcgg gcgcagcttc
    40801 agcagtcggt ggacggtttg cccttccagt ttgagcaaat ggcggcggac ggcctccggc
    40861 gcgtcaaaac cgttgattgc acggtgcagc gcgcgcgcca tatgtgccgc cgctttgccc
    40921 gtcggtgcgg caagcgcgat gttgggaaga ttttcgtctt caccgcaaat cagcgccagc
    40981 agtttggcaa ccgttgtcgt tttgcccgtt cccggcccgc cggtaatcac cataaaagac
    41041 tgcaacagtg ccaaggcggc ggcatcgcgc tgcccttcgc tgcccgtgcc ttgaaaccat
    41101 tttgcgaggt tttgcctcgc gcctgccgcg tcgggggcgg atgtgccggc tgccgccaag
    41161 cgttttatct cggcagccaa atcgtattcc aactgccaca tcctgcccaa aaacagcctt
    41221 ctgccttcca aaatcaaagg cgcggcggat gttccgacaa cgggtgcgag tgccgacagc
    41281 gcgtcagcct cgccaccgct caaacggata aacgaatgac cgttttgcaa tgcctgaaac
    41341 aggcgttcgg tgcagtttgc aagcacttcg tcgccggaac ccgcatagtg ttccaaaaaa
    41401 cggattgccg cccttgccgc cgcttgggca aattcatctg tctgccgttc cattttattc
    41461 cttatccaaa tgccgtctga aggcgtgggg cttcagacgg cgcggtgttg tttcggctgt
    41521 ttaggcgttt gcgccctgtt tggggtgcag gctgccgtct ttcatgacca tcacgcgctc
    41581 gaagcggccg gcgagttcgt cgtcgtgcgt tacgaccacc agccccgttc ccaattctgt
    41641 tttcagttcc agcatcatat ccaaaacatt cctggcgttc gcacgatcga gattgccggt
    41701 cggttcgtcg gcaagcaggc atttgggttg ggtaaccaag gcgcgtgcaa tggcggcacg
    41761 ctgccgctca ccgcctgaaa gttcgcccgc gcggtgcgtc gaacggtgtt tcagtccgac
    41821 cttttccagc atcgccattg ccgcctccgc agcctcttca cggctttttt tgccgatcag
    41881 aagcggcatc atcacatttt ccagtgccga aaattcaggc agaagatggt ggaactggta
    41941 cacgaaaccg agatggcggt tacgtaaatc gcccaaacgc cgctggttta aggtacgcaa
    42001 atcctcgccc atcagcagca ccctgccttc agacggcata tccagcccgc ccaaaatatg
    42061 cagcagcgtc gatttgccgc tgcccgaaga accgatgatg ccggtgcttt cccctgcgtg
    42121 gatttccaaa tccaagccgt gcagcacccg aacgtccaaa ccgccgtccc gatagcgttt
    42181 gcccacgcct tcgcatttca aaatcaactc actcataacg caaagcctcc gccggttggg
    42241 tttttgacgc gcgccggctc gggtagagcg tggcaacgaa agacagtccc aaagaaatgc
    42301 aggcaatcag ggcaacgtcg cccatatcga catcgctggg caggtagtcg ataaaataaa
    42361 cctgcgaatt gatgaggtgg acaccgagca ggttttcaaa aaacgccacg accctgccga
    42421 cgttccaacc caaaagcacg ccgcagacca cacccgccag cgtgccgaaa aagcctgaaa
    42481 acgcgccctg caccataaaa atcttcatca cgccagcagg ggaaagaccc aaagtccgca
    42541 aaatcgcaat gtccgcctgc ttttccgtaa ccgccatcac cagggaagag acaaggttga
    42601 acgccgccac agcgataatc agcgtcagga tgatgaacat catccgtttt tccagttcga
    42661 ccgcttcaaa atagctgcgg ttgctgtacg tccaatcgcg cacccaaacc gcgtcccttt
    42721 gcgcctccgg aatcagtgtt gccgtcaagg cgggagcgtt ttgcggatcg gcgagcttca
    42781 gccgcagccc cgcaacttcc ttatccaaac ggtacagcac gcgcgcgtct tggatatgcg
    42841 tcattgccaa tgagttgtcc acttcgtaaa cgcccgtctt aaccagaccg accacggtaa
    42901 actgtttcaa cctcggtacg actccggcgg gcgtaacatt gccctccggc gtgatgacgg
    42961 taactttatt gccgacttcc gcccccaaag cctccgccaa gccgacaccg aggataatgt
    43021 caaactcgcc cggaatcaga tcttcaaatt tgcctgccgg cattttgtcg ccgtattcca
    43081 ccactttgcg ttcttcagac ggcaaaatgc cgcgcatctg cacgcccctg atttcgcccg
    43141 cattggccag caatgcctga ttggaaacat agggcgcggc agccaaaata cctttgcggt
    43201 tttcggtaaa ccgaagcagg ttgcgccaat ccgtatccgt attatcgata tagccgattt
    43261 cggcgtgcgg cgcgacattc aggagctgcc cgcgtatttc tttctgaaag ccgttcataa
    43321 ccgacaagac gacaatcagc gcggttacgc ccaaggcgat tccggcaatc gaaaccatcg
    43381 tgataaacga cataaagccg ttgcgctttt tcgccctgag atacctcaag cctatccaag
    43441 cctctagaga aaacataacg ctaccttaaa aatgtctgca aacgtgccgc cccggacggc
    43501 ggtttggggg cggcggcaaa agtttattgt accgtaaaac ccaggcagcg tccgaacgcc
    43561 cgagtgcggg ggacggggcg gcagagcggg cggaaaactt gcacaacgcg tcaaactgcc
    43621 ctatcctttc cttcagaaaa accgtttctt gaggaaaaca atgaatatcc gaactgcttt
    43681 tgctttgtgc gccatcgcct tatccgccgc tgcggctgcc tacgccaaag aaatcaaaat
    43741 cgatgccaac aacacgcctt attccgaagc cgacgcgcaa aagctggcgg caacggcagt
    43801 cggtatgggt gttaaggaac ctatcagcct gaacggcggc agcggcagca ttaccgtgtc
    43861 cggcagcagc gcgacgcagt gcgtgttcaa agtcggcaac ggaggcgcat tgcagattca
    43921 agggctaaac tgcaagtaaa ccgcccggaa aaatgccgtc tgaaggcttc agacggcatt
    43981 ttgcattggc ggcgttatgc cccgccttct ttaatcaggc ggcgttcgta caccgcctgc
    44041 gccagcgttc ccgcatcgac atattccaat tcgccgccca agggaatgcc ctgcgacagc
    44101 ctgctgactt tgtaaggcag gtttttaaaa aactcggaca ggacatacgc cgtcgcattg
    44161 ccttctgcgg taaaagcggt tgcaataatg atttcttcga cttccccgcc gcccagccgt
    44221 tgcgccagcc tgtccaatgc gatggcggat acgtccattc ccaatgccgt attgatttgc
    44281 cccatcagga cgaaatacag cccgtcgtgg cagtttgccg cttccatatt cgacacgtcg
    44341 gcaggcatat gcaccaccat cagccgccgc ccgtcgcgtg tttcatcggc acaaatatcg
    44401 cacaatccgc cttcgcaaaa cgtgttgcac atcgcgcaat ggtaaacctg cttcaatgcc
    44461 gtctgcaagg catccaccag tttttcagcc tctttgcgct tgtgttggag caaatgatac
    44521 gctatccgct gtgccgattt cggcccgacg ttgggtaaaa ccttcagcgc gtcgatcaat
    44581 ccttggaagg catcttgttt tttgtggctc atcatattcc gccgtatggg aaaacggccg
    44641 gaatattccg accgttattt tgtcaacaaa agtgtcaatt actgaccgtc gccgttgtcg
    44701 accgattgcg ctcctttggt ctgtttgatt ttgccgttga aataacgtat caacaagtcg
    44761 aaagtattgg cagactgctg ttgcgccaaa gcctgttttg caagcggaag ctgtgcggcg
    44821 atatcatccg gcggggttac agcctgtact tcgacaatca cgggtgccgg cagaccgatc
    44881 agcctgacgt aggcgggttt gccgtttgcc ggttttgctt tcagcagttc cgcataagcc
    44941 tcgggcggca tggactgcct tgcctgctgt gcgcccaaaa cggacacttc cgaccatttc
    45001 acgtcaacag ccttgccgcc gttcagttgg gtaagcacgt cttttgcctt gttttcggca
    45061 agtttggcgg cttcggtacg gatataagcc tgacgtaccg cgtctttggc ttcggcaaac
    45121 ggcagggttt tctcttcgcg gacttctttg gcgcggacga cccacgcggt ttcgctgttg
    45181 atggtcagca cttcggaatt gtgttttttc ttcaatacgt cgtcgctgaa tacggcattg
    45241 atcaggtttt cgggcatacc ggacatttgc gcgtcctgcc tactcagcca agtttcttgg
    45301 gtttcgactt tcaaaccgct gtttttggcg gcttcggcaa gcgaggaagg atggttgaac
    45361 gcatcgtcgc ccaatttttc ttttgccttg ttgaagtcgg caaccgcctt tttcattttc
    45421 aattcgtttt cgacggcggc tttttcctgc tcgaaagaag gtttggcttc atttgccggc
    45481 aaacgcgcca cgcgctcttc aaatgcattt ttcacttccg tttcactgac ggtctgcttg
    45541 tctgcaaaat ccttcagatt caaggcgaca tattccaatt tgaccgcctg cggcagcaga
    45601 tagtcttttt tgttcgcatt ataaaatttc tgcaaatcgg cttcagacac tttgacttgg
    45661 gcgatgaact cgtcggggtt gaaagtgtgc gaacggatgg tgcggttgac ctgtgtcagc
    45721 ctgatcagct gttccgcctg cgcgtcgccg accaatacgc cgttttggac gaggtttacc
    45781 aaattctgca aggcaaactg atcgcggatt tcttcgacaa actggtcttc agacatatgg
    45841 cgttgggaaa ggtagcggtt taaaagcgcg tggtcgaatt tgccgtttgc gtcgtggaaa
    45901 ttgggatcgt ccacgataat ttgcttgatt tgttcggaag aaaccgaaat gcccatcagc
    45961 ttcgcgccct gtttcaggta ggcgcgttgc agcagggatt ggaacaccgc gtcgcgcgaa
    46021 gggccgccgc cgtccgcctg ttcgttctgt atggcgttgt tgatggagtg gtcgctgatt
    46081 ttttcgtcgc ccacttggac gatgtagtcg gcacccggat gggataccgt gctgaccccg
    46141 aagccgacga aggttaatgc aatcaggccc aaaaggactt gggcgggcgt tctgtatttt
    46201 tcgatggaat ggaacatatt ttaaatcggg atatagaatg ggaacgggaa attcaagtcg
    46261 ggtattgtaa cggtttttat ccctgtctgc acggggcttg ccggttgaag atgccgtcgt
    46321 aggtttcttc gctgaagccg gcgtaaacct tgccgccgca ctccaatacg ggacgcttga
    46381 tcaggctcgg catttcggac atcagtttga cggcctccgc cgtcgaggac agcacttttt
    46441 gctgtgtttc ggcatcgagt ttgcgccagc ttgtcccgcg tttgttgagc agggttgcca
    46501 aaggcacttg ttccagccac gagcagattt ccgcttcaga cggcctctgt tttttaaaat
    46561 cccgaaattc aaactccaag ccgtatccgg caagccggtt tttggctttt ttgaccgtat
    46621 cgcaatttgg gatgccgtga aggactatca tttggaaacc ttttgtctga aataataaaa
    46681 cggatatttt actataagtg tctgaaaatt tgcccgtctg tttcagacgg cggggcggtt
    46741 atgttacaat ccgaaaattc gaaaaattta atctcttgtt caataaaggc tttaccaatc
    46801 atgatttcta ccaacggcat caccatgcag ttcggcgcaa agccgctgtt tgaaaacgta
    46861 tccgtcaaat tcggcgaagg caaccgttac ggcttgatcg gcgccaacgg ctcaggcaaa
    46921 tccaccttca tgaaaatcct cggcggcgat ttggaacaga cggccggcga agtggcgatt
    46981 gaaaacggcg tgcgtttggg taaattgcgc caagaccagt ttgcctacga agacatgcgc
    47041 gtgctggacg tggtgatgat ggggcatacc gaaatgtggg cggcgatgac cgaacgtgat
    47101 gcgatttacg ccaatcccga agccaccgaa gacgactaca tgaaagccgc cgaactggaa
    47161 gccaagttcg ccgaatacga cggctacacc gccgaagcgc gtgccgccga actgttgagc
    47221 ggcgtgggca tttccgaaga tttgcacaat gcgaaaatgg cggaagtcgc cccgggcttc
    47281 aaactgcgcg tattgctggc gcaggcgctg ttctccaagc cggatgtatt gctcttggac
    47341 gaaccgacca ataacttgga cattaatacc atccgctggt tggaaggcgt gttgaaccaa
    47401 tacgactcca cgatgattat catcagccac gaccgccact ttttgaacga agtctgcacg
    47461 catatggcgg atttggacta caacaccatc accatctatc cgggcaacta cgacgactac
    47521 atgctcgcct ccgcccaatc gcgcgaacgc gccctgaaag acaatgccaa agccaaagag
    47581 aaactgcaag agctgcaaga gttcgtcgcc cgcttctctg ccaacaaatc caaagcccgt
    47641 caggcaacca gccgtctgaa acaggccgac aaaatcaaat cggagatggt cgaagtcaaa
    47701 ccttccaccc gtcaaaaccc gtatatccgt tttgaagccg atgaaaaagc caagctgcac
    47761 cgtcaggctg tggaagttga aaaactggcg aaacgctttg aaacccagtt gtttaaaaac
    47821 ctgaacttca tccttgaagc gggacaacgc ctcgccatca tcggcccgaa cggcgcgggc
    47881 aaatccaccc tgctgaaact cttggccggc gcgtacaacc ccgaatattc agacggcctg
    47941 ttgccggacg aaggcaccat caaatgggcg gaaaaagcca gtgtcggcta ctatccgcaa
    48001 gaccatgaaa acgacttcga cgtcgatatg gacctgagcg aatggatgcg ccaatggggg
    48061 caggaaggcg acgacgaaca agtcatccgc ggcactttgg ggcgtttgct cttcggcagt
    48121 aacgatgtcg tgaaaaaagt gaaggttctc tccggtggtg aaaaaggccg tatgctttac
    48181 ggcaaactgt tgctgttgaa acccaatgtc ttagtcatgg acgaaccgac caaccatatg
    48241 gacatggaaa gcatcgaatc cttgaacatg gcactggaaa aatacaacgg cacgctgatt
    48301 tttgtctccc acgaccgtca gttcgtttcc tccttggcaa cccaaatcat cgaactggac
    48361 ggcaaaggcg gatatgaaca ctacttgggc gattacgaaa gttacttgga gaaaaaaggc
    48421 gtagcataac cgccggttgg aacaatgccg tctgaagccg cttcagacgg cattgttgat
    48481 aactttaaaa taggaagcat atgcagactt atctcgtcgg cggtgccgtc cgcgattatc
    48541 ttttgggctt gcccgtcaaa gaccgcgatt gggtggtcgt cggcgcagac gcacaaacca
    48601 tgctggcgca aggcttccag ccggtcggca aagattttcc cgtgtttctc catcccgaaa
    48661 cacacgaaga atacgccctc gcccgcaccg agcgcaaaac cgccaaaggt tacgtcggtt
    48721 tcagtttcca cgccgacaaa gacgttacgc tggagcagga tttgatgcgc cgcgacctga
    48781 ccatcaacgc gatggcgcaa gatgcggacg gcaagattat cgaccctttc ggcggacaac
    48841 gggatttggc ggcaggcatt ttgcgccacg tttccccagc ctttgccgaa gaccccgtcc
    48901 gcatcctgcg tactgcccgc tttgccgcgc gttacaagtt tgaaatcgcc gaagaaacca
    48961 taaagctgat gcggcagatg gtggaaaacg gcgaagcgga cgcattggtt gccgaacgcg
    49021 tctggcagga gtttgcgaaa ggtttgatgg aaaaaaatcc gcgcaaaatg attgaagtgt
    49081 tgcgcgaatg cggcgcgctc aaagtcttgc tgcccgaagt caatgccctc ttcggcgtgc
    49141 cgcaacgcgc cgactaccat cccgaaatcg acagcggcat ccataccctg atgacgctgc
    49201 aacgcgccgc cgatatgggc ttgagcctgc ccgaacgcta tgccgccctg ctgcacgact
    49261 tgggcaaagc caaaacaccg tccgacatcc tgccgcgcca ccacggacac gacctcgccg
    49321 gtgtcgaacc cgtgcgcgaa gtcaatcagc ggctgcgtgc gccgaaacat tgcgccgagc
    49381 ttgccgaatt ggtttgccgt tggcacatta ttttccacca agtcggacag cttaaaagcc
    49441 aaaccattct gaacgttttg aaaaaaaccg acgctttcag acgacccgaa cgctttcaga
    49501 cggcattgaa cgtctgcatt gccgacacgc aaggccgtct gaaccgcgaa cacacgccct
    49561 acccgcaacg cgcgcactgg ctcgccttac tcgaagccgc caatcaggcg gattcgggca
    49621 aaatcgccgc cgaatgccgc gcacagggaa aagcgcacct tatcgccgaa caaatcgacc
    49681 gggcgcggct ggcacaaatc gccccattgc aaaaagcgtt tcgagcggcg caagacaaaa
    49741 cagaaaaaca ttaaaacgtc caatgcagcc a
//
LOCUS       DAWISC010000002        39302 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWISC010000002 DAWISC010000000
VERSION     DAWISC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.002"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(49..714)
                     /locus_tag="WIW87_000048"
     CDS             complement(49..714)
                     /locus_tag="WIW87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ5173273.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFIVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARSDSPFMLES
                     LKRRGVTEPDAVAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYAEMERILSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(824..1720)
                     /locus_tag="WIW87_000049"
     CDS             complement(824..1720)
                     /locus_tag="WIW87_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ5173274.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            1789..2946
                     /locus_tag="WIW87_000050"
     CDS             1789..2946
                     /locus_tag="WIW87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ5173275.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(3009..4010)
                     /gene="hemB"
                     /locus_tag="WIW87_000051"
     CDS             complement(3009..4010)
                     /gene="hemB"
                     /locus_tag="WIW87_000051"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ5173276.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4193..4477)
                     /gene="yhbY"
                     /locus_tag="WIW87_000052"
     CDS             complement(4193..4477)
                     /gene="yhbY"
                     /locus_tag="WIW87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ5173277.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQLVQHIGKLLVLWRKNIEA"
     gene            4587..5207
                     /locus_tag="WIW87_000053"
     CDS             4587..5207
                     /locus_tag="WIW87_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ5173278.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5271..7238
                     /gene="ftsH"
                     /locus_tag="WIW87_000054"
     CDS             5271..7238
                     /gene="ftsH"
                     /locus_tag="WIW87_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ5173279.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            7460..7891
                     /locus_tag="WIW87_000055"
     CDS             7460..7891
                     /locus_tag="WIW87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ5173280.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSTTLVEAFVKEADRRYA"
     gene            7884..8162
                     /locus_tag="WIW87_000056"
     CDS             7884..8162
                     /locus_tag="WIW87_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ5173281.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8215..8793
                     /gene="pth"
                     /locus_tag="WIW87_000057"
     CDS             8215..8793
                     /gene="pth"
                     /locus_tag="WIW87_000057"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ5173282.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSAEHR
                     QQIDDAIAKSLQAIPDIISGKWEEATRFLHSK"
     gene            8853..9059
                     /locus_tag="WIW87_000058"
     CDS             8853..9059
                     /locus_tag="WIW87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ5173283.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9056..9418
                     /locus_tag="WIW87_000059"
     CDS             9056..9418
                     /locus_tag="WIW87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ5173284.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9649..11064
                     /locus_tag="WIW87_000060"
     CDS             9649..11064
                     /locus_tag="WIW87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ5173285.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11183..11692
                     /locus_tag="WIW87_000061"
     CDS             11183..11692
                     /locus_tag="WIW87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5173286.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11855..13237
                     /locus_tag="WIW87_000062"
     CDS             11855..13237
                     /locus_tag="WIW87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ5173287.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(13257..14012)
                     /locus_tag="WIW87_000063"
     CDS             complement(13257..14012)
                     /locus_tag="WIW87_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ5173288.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14022..14738)
                     /locus_tag="WIW87_000064"
     CDS             complement(14022..14738)
                     /locus_tag="WIW87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ5173289.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14728..15555)
                     /locus_tag="WIW87_000065"
     CDS             complement(14728..15555)
                     /locus_tag="WIW87_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5173290.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVVRNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15840..16748
                     /locus_tag="WIW87_000066"
     CDS             15840..16748
                     /locus_tag="WIW87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ5173291.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            16828..20442
                     /gene="recB"
                     /locus_tag="WIW87_000067"
     CDS             16828..20442
                     /gene="recB"
                     /locus_tag="WIW87_000067"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ5173292.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            20481..20840
                     /locus_tag="WIW87_000068"
     CDS             20481..20840
                     /locus_tag="WIW87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ5173293.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            21082..21561
                     /locus_tag="WIW87_000069"
     CDS             21082..21561
                     /locus_tag="WIW87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ5173294.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(21837..23216)
                     /gene="radA"
                     /locus_tag="WIW87_000070"
     CDS             complement(21837..23216)
                     /gene="radA"
                     /locus_tag="WIW87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ5173295.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(23402..24466)
                     /locus_tag="WIW87_000071"
     CDS             complement(23402..24466)
                     /locus_tag="WIW87_000071"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ5173296.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(24620..25060)
                     /locus_tag="WIW87_000072"
     CDS             complement(24620..25060)
                     /locus_tag="WIW87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173297.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            complement(25093..26316)
                     /locus_tag="WIW87_000073"
     CDS             complement(25093..26316)
                     /locus_tag="WIW87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ5173298.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            complement(26313..27647)
                     /locus_tag="WIW87_000074"
     CDS             complement(26313..27647)
                     /locus_tag="WIW87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ5173299.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NEKTASEPAAASDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            complement(27662..28423)
                     /locus_tag="WIW87_000075"
     CDS             complement(27662..28423)
                     /locus_tag="WIW87_000075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ5173300.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(28444..28863)
                     /locus_tag="WIW87_000076"
     CDS             complement(28444..28863)
                     /locus_tag="WIW87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173301.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(29114..29434)
                     /locus_tag="WIW87_000077"
     CDS             complement(29114..29434)
                     /locus_tag="WIW87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ5173302.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(29457..30083)
                     /gene="upp"
                     /locus_tag="WIW87_000078"
     CDS             complement(29457..30083)
                     /gene="upp"
                     /locus_tag="WIW87_000078"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ5173303.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(30189..30500)
                     /gene="grxD"
                     /locus_tag="WIW87_000079"
     CDS             complement(30189..30500)
                     /gene="grxD"
                     /locus_tag="WIW87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ5173304.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(30601..30816)
                     /locus_tag="WIW87_000080"
     CDS             complement(30601..30816)
                     /locus_tag="WIW87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ5173305.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            complement(30842..31621)
                     /locus_tag="WIW87_000081"
     CDS             complement(30842..31621)
                     /locus_tag="WIW87_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ5173306.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(31626..31976)
                     /locus_tag="WIW87_000082"
     CDS             complement(31626..31976)
                     /locus_tag="WIW87_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ5173307.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(31980..32957)
                     /locus_tag="WIW87_000083"
     CDS             complement(31980..32957)
                     /locus_tag="WIW87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ5173308.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(32991..34103)
                     /locus_tag="WIW87_000084"
     CDS             complement(32991..34103)
                     /locus_tag="WIW87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ5173309.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRT
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            34289..34990
                     /locus_tag="WIW87_000085"
     CDS             34289..34990
                     /locus_tag="WIW87_000085"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ5173310.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYAWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            35134..36927
                     /gene="lepA"
                     /locus_tag="WIW87_000086"
     CDS             35134..36927
                     /gene="lepA"
                     /locus_tag="WIW87_000086"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ5173311.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVIYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            37070..38089
                     /gene="lepB"
                     /locus_tag="WIW87_000087"
     CDS             37070..38089
                     /gene="lepB"
                     /locus_tag="WIW87_000087"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ5173312.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(38245..39150)
                     /locus_tag="WIW87_000088"
     CDS             complement(38245..39150)
                     /locus_tag="WIW87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5173313.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKRQSSH"
BASE COUNT         9416 a        11144 c         9945 g         8797 t
ORIGIN      
        1 attaaaaaat gccgtctgaa aatccttgcg ttttcagacg gcattgcctt atgcccaaat
       61 aacggtttct tccaagggcc tacgcgcttt cgtggcgatt tcctgaacgc ggtagccgaa
      121 tgtcgcggcg acggacacgc cccattctgc cgcatcgaac aaaccaaact gcccggacaa
      181 tatgcgctcc atctcggcat agttgaaacc ttccaccggg caggaatcga tacctgccat
      241 cgccgcaccc gtcatcatgt tggctaacgc gatataggtc tgacggcaac accagtcaaa
      301 caaggcgcga gaatcgtcca aaatcttgat gtcgtcagct tgaaacgcct gataccttgc
      361 caaagatttt gctacggcat ccggttcggt aacgccgcgc cgtttgaggc tttccaacat
      421 aaacgggctg tcggagcggg catttttctt cgccaaaaac accaccaaat gactggcggt
      481 atccaaagca tccgccatac cccaagaaaa cggcttgatt gcctgtcgga tttcagggtt
      541 ttgaaccaca ataaactgcc aaggctccga accgaccgaa ctgggcgaca aacgcccgag
      601 ttctaaaata aactgaaaat cctcggcact gattttgcgt gccgcatcgt aatgccggca
      661 tgatttacgg tttttaaatg cggatagaac ctgctctttg cttaatactg tcatcgtgca
      721 tccttttatt acttgtattg caaattgatt tccgatacgg cggcagattc gccaataacg
      781 cccaaacgcc tttcagacgg catttggttc atcgcaaacg cgcctacggg taggcgatat
      841 aggcataagc cgaatggttg tggatagact cgaaattctc gctctcgacg acgaaactct
      901 tgatgcgttt gtcggcaatc agcgaagtag cgacatcgcg caccatatct tccacgaatt
      961 tcgggttttc gtaggctttt tcggtaacgt atttttcatc ggggcgtttg agcaggccgt
     1021 agagttggca gctcgcctgc gcctccacat aatcgatgac ttcctcgata ccgacttcgg
     1081 catcggcagt caggctgacg gtaacgtgcg aacgctggtt gtgcgcgccg tattgggaaa
     1141 tttctttgga acacgggcaa agcgaggtta cgggaatcat gaccttcata ctgtggccgt
     1201 atgccccgtc tttgatttcg cccgtgagac agacatcata atccagtaag gaccgaatac
     1261 cggaaaccgg ggcggttttc ttgcggaaaa atgggaaaga aacgctgatt ttgccggcgc
     1321 gggaatctaa aagcgcgacc atttcggtag tcagcttgcg caattgtgca aaatccaagg
     1381 cttcggcatg ttgctccatc aatgcgacaa aacgcgacat atgcgtcccc ttctgctcag
     1441 caggcaggta aaccgtcatg gtcagacggg caatggtgga ctggatgcct tctgcagttt
     1501 gcagggtaat cggaaagcgc aggtctttga taccgacctg attgatcggc agattgcgca
     1561 aatctcggct ggattgcacg tctgcaatag tgttcatgag ttgtttttcc ttaatattca
     1621 tgtaattgtt gagatgtgtg catggtatgg cgtttccgcc ggggaatata ccgtcaatct
     1681 gcaaatgcga gattatatca ctcgctttta gcagctgcat tgattgtttc tatttttcag
     1741 atagtgataa aatcttcgat tattcacatc tacataggca gagaccaaat gaaattcgcc
     1801 acgaaagcca ttcattccag ctacgattgc gacgaacaca accgcgcgct gatgccgccg
     1861 atttatcaaa acagtatgtt tgcgttgcac gagattggcg aaaatgtgcc ttaccgttat
     1921 tcgcgcctga gcaacccgac ccgtcagatt ttagaagaca ccgttgccga tttggaacac
     1981 ggtgcggcag gttttgcgtt ttccagcggt atggcgggaa ttgatgccgt atggcgcact
     2041 ttcctgcgcc cgggcgatac cattgtcgcc gtcgccgata tttacggcgg cgcttatgat
     2101 ttattggtcg atgtttatca aaaatggggg gtgaacgttg tttttgccga tttaggcaat
     2161 ccggataatt tggacgaact gcttaaagcg cacaaggtca aactggtttg gctggaaacg
     2221 ccgtccaatc cacttttacg cttggtagac atcaaagtcc ttgccgcaaa agccaaagca
     2281 gccggtgcgc tggtcggtat cgacaacact tttgccacgc cgtatctgca acagccgttg
     2341 gatatgggtt gcgattttgt attccattcc gctaccaaat atttgtgcgg ccattccgac
     2401 gtgttgatgg gcatcgtcgt tgccaaaacc aaagaactgg cgcagccttt gcacgatatg
     2461 atggtgcata ccggcgcggt tgccggcccg ctggactgct ggctggtgtt gcgcggcatc
     2521 aaaacactgg ctctgcgcat gaacgcccat tgccaaaacg cactcgaaat cgcgcgccgt
     2581 ttggaagccc atcctgccat tgaaaaagtg ttccatcccg gcctgccgtc tcacgaacat
     2641 tacgaactgg cgaaaacaca aatgcccaaa ggcatcggcg gcgtggttac ggtttatctc
     2701 aaaaacgaca cgcgtgaagc ggcaaacagc gtgattaaaa acatgaaact ggtcaaaatg
     2761 gcttccagcc tcggcggtgt ggaaagtttg gtcaaccatt gctattccca gtcccacagc
     2821 ggcgtaccgc atgatgtgaa aatggaaatg ggcatcaaag tcggtctgct gcgtttctcc
     2881 atcggcatcg aagacgcgga cgatatttgg aacgatattt ccgccgcact cgatacaact
     2941 ttgtaaactg taaaaatgcc gtctgaaacc atggtttcag acggcatttc aattaacccg
     3001 gccgaaaatc aacgcttcaa catctttgcc gcctcaatag cgtaataggt caaaatcccg
     3061 tccgcacccg cacgtttgaa tgccagcagg ctttccaaaa ccactttgcc gccgtccagc
     3121 cagccgttgg caatcgctgc ctgcaacatc gcgtattctc ccgaaacctg ataggcataa
     3181 gtcggcacac cgaactcgtc ctttacgcgg cggacaacgt ccaaatacgg caaaccgggc
     3241 ttgaccatta ccatatccgc accttcctga atgtccaacg ccacttcgtg caacgcctca
     3301 tcggtatttg ccggatccat ctggtaggtc tttttatctg ccttgcccaa attgcccgaa
     3361 ctgcctaccg catcacggaa agggccgtaa aatgcagaag catatttggc ggaatacgcc
     3421 ataatccgcg tatggatatg cccggcatcc tccaacgcct cgcgaatcgc accgatacgc
     3481 ccgtccatca tatcggaagg ggcaaccacc tgcgcgcccg cttcagcgtg gcacaaagcc
     3541 tgcttgacca aaacctctac ggtttcatcg ttcatcacat aaccgttttc gtccgtcagc
     3601 ccgtcctgac cgtgaaccgt ataaggatcg agcgcgacat ccgtcataat gcccagttcg
     3661 ggaaacctct cgcgcaaggc gcggacagtt gacggcacga gtccttcggg attgtacgcc
     3721 tcctgcgcac gctcggtttt gtttgccgta accacgggga acagtgccaa catcggaata
     3781 ccgagcttta ccgcctcttc cgccgtaaac agcagcctgt ccaaactttg acgcttcaca
     3841 cccggcatag aaggcacatc ctcctcgcgc gccgacccct ccaatacgaa caccggataa
     3901 atcaaatcat cggcggtcag cgtgtgttcg cgcatcaggc ggcgtgaaaa atcgtccctg
     3961 cgcatacggc gcatacgcga agccggaaca ttgcggtaag gaaactgcat aagccctcca
     4021 atcatcctgt caacaattca aaccatacgg aagccgccgc aacttgcaga cagcatcggg
     4081 cggcaatcaa aatccgtttt caccggaaag ggtttcggcc cgccgaaatg ccgcgccgaa
     4141 ggacggcgtc ccgcccgtta aagaacaata gcaaaacaac tgcttcaggc tgtcaggctt
     4201 cgatattctt acgccacaat accaaaagtt ttccgatatg ctgaaccagt tgcgcatcaa
     4261 ccgcctcaca taaggcagtg cagatttcga tacgttcggc acggtcgtcg ccgaatacgc
     4321 gcactttaat cagctcatgc gccgtcaatg ccgcatcggt ttccttgatg accgcgtccg
     4381 tcagaccctg ctgaccgacc atcacaacag gatggagatg gtgcgcgcgc gctttcagtt
     4441 ccaaaatttc tttggtgttc aatttggtat ccgtcatttt cctactgctt gaataaaaag
     4501 aataatgcgg cattgtacgc gatttggcac gacatcggca aaattaacgt acaatacgcg
     4561 gtttccattc cgacccgcga accactatgg ctgtacgttc caaatcctca aaagcgtggc
     4621 tgcacgaaca cgtcaacgac cactacgtcc atatggcgca aaaagacggc taccgcgccc
     4681 gtgccgcata caaacttttg gaaatcaacg aaaaagacaa attaatcaaa cccggcacgg
     4741 tacttgccga cttgggcagc gcgccgggaa gctggtcgca ggttgccgcc aagctgacgg
     4801 gtacttccgg agcagttttc gccttggaca tcctgcctat ggaagccata gggggcgtct
     4861 ccttcattca gggcgacttc cgcgagaacg acgtactggc acaattcgaa accttgttgg
     4921 acaaccgccc gctcgacctt gtaatttgcg atatggcgcc caatatgtcg ggaaacgccg
     4981 taagcgatca ggcacgcagc ttttatctgt gcgaactggc tttagacttc gcctcgcaac
     5041 acctgaaaac cggcggcagc tttttggtca aagtctttca gggtgcaggc tatcaggaat
     5101 acatggcagc catgcgcgaa attttcggca cggtgcagac gcgcaaaccc gaagcctcgc
     5161 gcaatcgctc cagtgagatt tatttattgg gcaaaaataa acgctgacaa tacagacggc
     5221 gtgctttaca atcatttccg ttttacacct caattatgga gccttgctaa gtggggaaca
     5281 cctttaaatc aatccttgtc tgggtcgcct tgggtatcgg cctgatggct gcgttcaacg
     5341 ctttagacgg taaaaaagaa gacaacgggc aaatcgaata ctctcagttc atccaacagg
     5401 tcaacaacgg cgaagtatcc ggcgtcaaca tcgaaggatc cgtcgtcagc ggctacctga
     5461 ttaagggcga gcgcaccgac aaaagcactt tcttcaccaa cgcgcctttg gacgacaacc
     5521 taattaaaac actgctcgac aaaaacgtcc gcgtaaaagt aacgccggaa gaaaaaccga
     5581 gcgcgctggc tgccctgttt tacagcctgc tgcccgtcct gctgctgatt ggcgcatggt
     5641 tctacttcat gcgtatgcag acgggcggcg gcggaaaagg cggcgcattc tcattcggta
     5701 aaagccgcgc ccgcctgctg gacaaagatg ccaacaaagt gacctttgcc gatgtcgccg
     5761 gctgcgacga agccaaagaa gaagtacagg aaatcgtcga ttacctcaaa gcgccgaacc
     5821 gctatcaaag cctgggcggg cgcgtgccgc gcggcatcct gctggcgggc agcccgggta
     5881 cgggtaagac gcttttggcg aaagcgattg caggcgaagc cggcgtgccg ttcttcagca
     5941 tttcaggttc cgactttgtc gaaatgttcg tcggtgtcgg tgcgagccgc gtccgcgata
     6001 tgttcgagca ggcgaagaaa aacgccccct gcatcatctt tatcgacgag attgacgcag
     6061 tcggccgcca acgcggcgca ggtttgggcg gcggcaatga tgagcgcgag caaacattaa
     6121 accaattgtt ggttgaaatg gacggttttg agagcaatca gactgtaatt gtgattgcgg
     6181 caaccaaccg ccccgacgta ctcgatcctg cgctgcaacg ccccggccgt ttcgaccgcc
     6241 aagtggttgt ccccctgccg gacatccgag ggcgcgaaca gattttgaac gtccattcta
     6301 aaaaagtgcc tttggacgaa tctgtggatt tattgtccct cgcgcgcggc acgccgggtt
     6361 tttccggcgc ggatttggcg aacttggtca acgaagccgc cctgtttgcc ggccgccgca
     6421 ataaagtcaa agtcgatcag agcgattttg aagacgccaa agacaaaatc tatatgggtc
     6481 cggaacgccg cagtatggtg atgcacgaag acgaaaaacg tgcgacggcg tatcacgaat
     6541 ccggacacgc gattgttgcc gaaagcctgc cctttaccga ccccgtccac aaagtaacca
     6601 ttatgccgcg cggacgtgcg ctgggtctga cttggcagct tccggagcgc gaccgcatca
     6661 gtatgtataa agatcagatg ttgagccagc tctccatcct gttcggcgga cggattgccg
     6721 aagacatctt cgtcggacgc atctccaccg gcgcatcaaa cgactttgaa cgcgcaaccc
     6781 aaatggcgcg cgaaatggta acgcgctacg gcatgagcga caaaatgggc gtgatggttt
     6841 atgcggaaaa cgaaggcgaa gtcttcttgg gacgcagcgt aacccgttcg caaaacattt
     6901 ccgagaaaac ccagcaggac atcgacgcgg aaatccgccg gattttggac gagcaatatc
     6961 aggttgccta caaaatcctc gatgaaaacc gcgacaagat ggaaacgatg tgcaaagccc
     7021 tgatggaatg ggaaaccatc gaccgcgatc aggtactgga aatcatggcg ggcaaacaac
     7081 ccagcccgcc caaagattac agccacaacc tgcgcgagaa tgcggacgcg gcggaagata
     7141 acgcgccgca cgctccgact cgggaagaaa ccgaagcacc tgccccggca gacaccgctt
     7201 cgacagagtc cgagcagcag cctgaaaaca aggcttaact tcccgaacaa acggcagccc
     7261 gcaagctgcc gttttcccat cccgttttca gacggcattt tccactcaat gccgtctgaa
     7321 acgccaaagc cgtatcggtt tttacgaatc tgcgatatgg cggtttaaat tgaaatcaga
     7381 acggggcaag gctgtaccgg tttaaaatta aaccgctata ataccgccct tcgaatacac
     7441 gcccgcattg gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc
     7501 ggacaaaatg ttcgagctgg tcgacaaggt tgaagactat ccgcactttc tgccgtggta
     7561 cagcaagacc gaagtcatcg ggcgtagcgg caacgaactg aaggcgcggc tgtttatgga
     7621 ttatatgcac gttcgccaat cgtttgccac gcacaaccgc aacattccgg gcagggaaat
     7681 ccgtatggaa ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga
     7741 tttgggcgac gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt
     7801 tttgtccgcc ttaatttccc ccgtcttcaa ccacctttcc accacgctgg tcgaagcgtt
     7861 cgtcaaagag gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgcccgacc
     7921 gacaggtttt gaaaaccatg cagcttgccg agggaacaac cgtccgcgcc gccgcactgc
     7981 aaagcggttt ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt
     8041 tcggcaaagc cgtcaaagac gacacgccgc tgcgcgacgg cgaccgcatc gaagtgtacc
     8101 gcccgctgtt gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat
     8161 aaccatgccg tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca
     8221 aacacaatca aaatggttgt cggcttgggc aacccgggca aagaatacga acagacacgc
     8281 cacaatgcgg gtttttggtt cctcgacgaa ctggcgtgga aatggaaggc ttcgtttaaa
     8341 gaagaaaaaa aattcttcgg cgaagtcgcc cgtgctaccc tgcccgacgg cgatgtctgg
     8401 ctgctcaagc cgaccacgtt catgaaccgt tccggacagg cagttgccgc ccttgcgcag
     8461 ttttataaaa tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc
     8521 ggacggatca aattcaaact cggcggcggc aacggtggac acaacggctt gaaagacatt
     8581 caggcacggc tcggcacggc cgactattac cgcctgcgcc tcggtatcga ccacccgggc
     8641 gaccgcaacc tcgtcgtcgg ctatgtcctg aacaaaccca gtgcggaaca ccgccaacag
     8701 attgatgatg ccatcgccaa atccctgcag gccatacccg acatcatttc cggcaaatgg
     8761 gaagaagcaa cacgtttcct gcacagcaaa tgacccaatg ccgtctgaag ccctttcaga
     8821 cggcattccc gccatcagcg agaacccgca ctatgtccga actcaaccgg ctcatccaaa
     8881 ccgaatccgt tgccgtcatt gaagaaactt taaattttct gctctacgaa tgcagcatag
     8941 acgatgcccc ctccgccgaa gaaattgccg tttggcgcga tatgctggcc gcacgcggcg
     9001 gaaaattcct gcgcctgtcc aaaatctgcc aaacgtggct ggacgaggag gcggcatgaa
     9061 gctgccgcgc aaccgcttca gcctgctttc cgcattgtgg tttgccggcg gcatctattc
     9121 gctgctcttc aaagctgccg acaccgcgcc gccgccgttt ccgcatttcg acaaagcagc
     9181 acaccttgcc ctgtttttcg cacaaatctg gcttttgacc aaagcattca aaaccggaaa
     9241 acttcccatc ccctaccgca gcctgatggt ctttgccctc tgtttcgccc tcttcagcga
     9301 atgcgcgcag gcatggttta ccgcaacgag aaccggcagt ttgggcgatg ttcttgccga
     9361 tatggcaggt acggttctcg cactctttgc cgcccgcgcc gccgaccgcc cggactgaat
     9421 cggtttattt tccccaaaca ggatgcacct cttcccgata catcctattt ttccccttac
     9481 atatcaatcc catctcataa acaaaaccga caaatcgttt acaatatatt tacactacat
     9541 cagattacaa atatactcga cccagttgca gaatcggcgg tttcacaaac cgtttcggat
     9601 tgcacgacac aaaagaaaaa ccgattttat tgccttaaag gagtattcat gaacctgcat
     9661 gcaaaggaca aaacccagca tcccgaaaac gtcgagctgc tcagtgcgca gaagccgatt
     9721 accgacttta agggtctgct gaccaccatt atttccgccg tcgtctgttt cggcatttac
     9781 cacatcctgc cttacagccc ggatgccaat aaaggtatcg cgctgctgat tttcgttgcc
     9841 gcactttggt ttaccgaagc cgtccacatt accgtaaccg cactgatggt gccgattctc
     9901 gccgtcgtac tcggtttccc cgacatggac atcaaaaagg cgatggctga tttttccaac
     9961 ccgattatct acattttttt cggcggcttc gcgcttgcca ccgccctgca tatgcagcgg
    10021 ctggaccgta aaatcgccgt cagcctgttg cgcctgtcgc gtggcaatat gaaagtggcg
    10081 gttttgatgt tgttcctcgt taccgccttt ctgtccatgt ggatcagcaa caccgccacc
    10141 gccgcgatga tgctgcctct agcaatgggt atgctgagcc acctcgacca ggaaaaagaa
    10201 cacaaaacct acgtcttcct cctgctcggc atcgcctatt gcgccagcat cggcggcttg
    10261 ggcacgctcg tcggctcgcc gcccaacctg attgccgcca aagccctaaa tctggacttc
    10321 gtcggctgga tgaagctcgg cctgccgatg atgctgttga ttctgccctt gatgctgctc
    10381 tccctgtacg tcatcctcaa acctaatttg aacgaacgcg tggaaatcaa agccgaatcc
    10441 atcccttgga cgctgcaccg cgtgatcgcg ctgttgattt tccttgccac agccgccgcg
    10501 tggatattca gctccaaaat caaaaccgcc ttcggcattt ccaatcccga caccgttatc
    10561 gccctgagtg ccgccgtcgc cgtcgtcgtc ttcggcgtgg cgcaatggaa ggaagtcgcc
    10621 cgcaataccg actggggcgt gttgatgctc ttcggcggcg gcatcagcct gagcacgctg
    10681 ttgaaaacat ccggcgcgtc cgaagccttg ggacagcagg ttgccgccac cttttccggc
    10741 gcgcccgcat ttttggtgat actcatcgtc gccgccttca ttatttttct gaccgagttc
    10801 accagcaaca ccgcctccgc cgcattgctt gtaccgattt tctccggcat cgctatgcag
    10861 atggggctgc ccgaacaagt cttggtattc gtcatcggca tcggcgcatc ttgtgccttc
    10921 atgctgccgg ttgccacacc gcctaacgcg attgtgttcg gcacgggctt aatcaagcaa
    10981 cgcgaaatga tgaatgtcgg catactgctg aacatcctct gcgtagtatt ggttgctctg
    11041 tgggcttatg ctgtactgat gtaaaccatc gacctaaaca acaagaccgt ctgaaagaat
    11101 atttttcaga cgaccttgaa gtttgtcgtt cagacacaat ttgtcgaatc attcaaaacc
    11161 ggattctaac gaaaggaaac ccatgattat cctgcacacc aacaaaggcg acatcaaaat
    11221 cgaactcgat ttcgacaaag cccccgttac cgccaaaaac ttcgagcaat acgtcaaaga
    11281 cggcttctac gacggcgtaa tcttccaccg cgtcatcaaa ggcttcatga ttcaaggcgg
    11341 cggcatggat gaaaacatga acgaaaaaga aacccgcgat ccgattcaaa acgaagcgtc
    11401 caacggcctg cccaacgata aatacaccat cgccatggca cgcacttccg acccccattc
    11461 cgccagcgcg caattcttca tcaacactgc cgacaacgct ttcctgaact tccgttctaa
    11521 agagctgtac ggcaaaaccg tcgtccaaga ctggggctat gcagtattcg gtaaagtcgt
    11581 tgacggtttt gacgttgtcg atgccatcga aggcgtatcc accaaacgcc acggctacca
    11641 tgacgacgta ccgagcgaac ctgtcgtcat cattaaagcc gaagcggtat aaaccggcaa
    11701 tccgaaagca gcctgcacaa aggctgcttt ttttgtttca ggcggcataa aatccaagca
    11761 catatgccgt ctgaacacct cccgcaagcc ttcggacaat gttgcaacgt gttaaaatag
    11821 cctcctttcc tgaaaccaca acaaggaaac catcatggca aatattgccc gcgacatctt
    11881 caaagcctac gacatccgag gcattgtcgg caaaaccctg actgacgatg ccgcctatct
    11941 catcggcagg gccatcgccg ccaaagccgc cgaaaaaggc atgacccgca tcgcgctcgg
    12001 acgcgacgga cgcttgagcg gtcccgaact gatggagcac atccaacgcg gctttaccga
    12061 cagcggcatc gacgtcctca acgtcggtat ggtcgccacg cccatgctct attttgccgc
    12121 tatcaacgaa tgcggcggca gcggcgtgat gattaccggc agccacaatc cgcccgatta
    12181 caatggtttc aaaatgatgc tcggcggcga cacgctcgca ggcgaagcca ttcaagaact
    12241 tttagccatt attgaaaaag acggttttgt tgccgccgac aaacaaggca acgtaaccga
    12301 aaaagacatc tccggcgaat accacaacca catcgtcggc cacatcaaac tcaaacgccc
    12361 catgaaaatc gccatcgacg caggcaacgg cgtaggcggc gcattcgcag gcaaactcta
    12421 caaaggcttg ggcaatgaag ttaccgaact tttctgcgaa gtggacggca acttccctaa
    12481 tcaccacccc gacccttcca aaccgaaaaa cctgcaagac ctcatcgtcg agctcaaaaa
    12541 cggcgaggcc gaaatcggct tagcatttga cggcgatgcc gaccgcttgg gcgtggttac
    12601 caaagacggc aacatcatct atccggaccg ccaactgatg cttttcgcgc aagacgtgtt
    12661 gagccgcaat cctaaagcca aagtgatttt cgacgtcaaa tccacccgtc tgcttgcgcc
    12721 ttggattaaa gaacacggcg gcgaacctgt gatggaaaaa accggccaca gctttatcaa
    12781 atccgccatg aaaaaaaccg gcgcgccggt tgccggcgaa atgagcggac acatcttctt
    12841 caaagaacgc tggttcggct tcgacgacgg tctgtacgcc ggcgcacgcc tcttggaaat
    12901 cctgtctgcc tccgataatc cgtccgaagt gttaaacaac ctgccgcaaa gcatttccac
    12961 gcccgaactc aacatcgccc tgcccgaagg cagcaacggc catcaggtta tcgacgaact
    13021 cgccgccaaa gccgaatttg aaggcgcaac cgaaatcatc accatcgacg gtctgcgcgt
    13081 tgaatttccc gacggcttcg gtctgatgcg tgcttccaat accacgccga ttctggtgtt
    13141 gcgttttgag gcggatacgc aagaagccat cgagcggatt caaaaccaat tcaaagccgt
    13201 catcgaaagc aatccgaatc taatctggcc tctgtaaaca caggaaaaat gcttaatcaa
    13261 atcttggtag attggatttg gcttaaaaat ctccgcgtcc gttcgtgttt ggggtggtcg
    13321 aacaactctt tcgggctgcc ctgctctacg ataacgccgc cgtccatcac gacaacggtc
    13381 gtggcaactt ccagcgcgaa cttgatttcg tgggtaacga cgaccatcgt ccaaccttcc
    13441 cgcgccaatt ccttcatggc gttcaacacg tcttgcacca actcggggtc aagcgcggaa
    13501 gtgggttcgt caaacaacat cagctcgggc tgaatcgcca atgctcgggc aatgccgacg
    13561 cgctgctgct gaccgccgga aagctggtag ggatacaaat ccactttgtc gcccaagccg
    13621 actttttcca gcagtttcag agcctcttcg cgcgcttggg cggcaggctt gccctgtacg
    13681 gcaaccggtc cttccatcac gttttccaag gcggttttgt gcggaaagag gttgtattgt
    13741 tgaaacacca tgcctgattt gcggcgcagt gccaaaatat cgtgtttgct tggtttttta
    13801 gaaaaatcga ttttcagcgg tcgctcgttg tcgaactcga tttgtccgtc ttcgggcatt
    13861 tccaacgcgt ttaggcagcg cagaaacgtc gttttgcctg agccggaagg cccgaggatg
    13921 acgaccacct gccctttgca cacatccaaa tcgatgccgc gcaaaatagt gttttcgcca
    13981 aaggtcttat ggatattgcg gattttaatc atgacaactc cttatttggc gacgtagcgg
    14041 tcgaaacgtt tttccaaacg cgcctgaatc aggaacagca ctttacaaaa acaccagtaa
    14101 accaaagcgg cttcgatata gacgggcaaa aagtcataag tgcggtttgc cgtttcctgc
    14161 gcgacgcgga ataattccgt taccgtcacg actgccgcga gcgaggtgtt tttaaacaaa
    14221 ccgataaact cgttgctcaa aggcggcacg gcaacgcgga atgcctgcgg cgcgacaatg
    14281 cggcggaacg tctgcatata ggtcatgccg atggagaaac ctgcttccca ttggccttta
    14341 ggtacggaca aaattgccgc gcgtatggtt tcggaagcgt atgcgccgac attgagcgaa
    14401 aagccgatga tggcggcagg aatcgggtcg atatagatgc cgacggaagg cagcccgtaa
    14461 aacacaatca caagctgaac caacagcggc gtaccgcgaa tgacggaaat ataaaattcc
    14521 accaatttca gcaggatttt ccgcacgatg ccgccggcgg gcataatccg caccaaagcc
    14581 acggctaccg caatcatcat accgataacg aaagaagctg ccgccaaagg cagagagacc
    14641 gcgaagccgg ctttgaccat aggcaaaaac gcgctgacaa tcatatcggc gcgtgtttcc
    14701 gtcataaacg gcagcgaagc aaggaaatta ttgaacactg atgtcttttc cgaagaattg
    14761 ttcgcccagt tttttcagcg taccgtcggc tttcagctcg ttgattgccg tactgaattt
    14821 cgccacggct tcgtcattgc ccttgttgac aatcaggccg gaaccgactt tttcatcggc
    14881 aggtgcggac caaacgattt tcacgcccgc attcgggttt ttcttcagat agtccaaaac
    14941 cgccaattcg tcgttcaggg ttgcatcggc acgtttttgt tcaatcaggg tcagcgattg
    15001 cgccaaaccg tcaacagcca ccaaatctgc gcctgcagct ttggcttttt cgccgtagtt
    15061 gctggtcagg gattgtgcgg ttttcacgcc tttgatgtcg gctatagatt tgatgttgct
    15121 gtcgttacgg acaaccaata cggcaccgct ccagctgtaa ggatcggatt tgtcgaatgt
    15181 cgcttggcgt tcggggctgg tcagaccgac ttggtttgcc accacgtcga aacgccccgc
    15241 cttcaaaccc gccatcatcg aatcccattg cgtttcttta aactcgactt tcacgcccag
    15301 tttttccgcc acggcgcggg ttacttccac atcgtaaccg gtcagtttgc cgtccttgtc
    15361 gtggtaggta aacggtgcgt aagtgccttc cgtgccgacg gtaaccgtgc ctttattgtt
    15421 gatgcgctcg attaaagaac cggaaactgc cgattgtgca ggcgcggaag atgctccgct
    15481 gccgccttcc gaaccgccgc aggccgccaa aaccaatgcg gcaataccgc cgagtacgaa
    15541 ttttttcaac atcatcctct cctgtaaaaa gtgtgcaagt gcggcattct aagtgaaatt
    15601 gcggcaaaca caaagaatac tttttttata tggttattcc aaatcggaat ggaaaagata
    15661 tgccgaagcc gccggaagcg gaaataaagg gggcgggaaa ggcggcgggc ggcacgaaac
    15721 cgaaaagcgg catcaggtat gtaaacggcg gattcacgca cgccgttccg gcaaaaatcc
    15781 gcctgccgat tcggtgctat aatctatccg aaacagatga caccaaaaag gaacatctca
    15841 tgaacacgcc ccaatccgcc atcattcccg accacgccca agccggcatc tttatcgaag
    15901 ccgacttcgc cgccaatcgc ctcaacgata ttaaagccgc ctgccgcgct tcgctcgacg
    15961 cgttaactgc cttgaaagcc cgctttccgg acgatatttt gggtctgacc atcgccttcg
    16021 gcagcaaagc ctgggcaaca ttcggacata cggacgaagg cagtgaaatc aaacccttct
    16081 ccgaaatggg caacgggctt gcgccgtcca cgcagcacga tatgtccatc cacatccaat
    16141 ccttccgcca aaacgccgcc tacgcgcttg cccaatccgt tttgggcgcg ttcggcgaca
    16201 gcatatgcgt cgcgtccgaa gaacacggtt tgcgcctgta tcaggatcgc gggctggacg
    16261 gtttcgtcga cggcacggaa aacccgcagg gcgatgaaaa tgtccgcgaa gtcgccatca
    16321 tccccgaagg aaaacccgat gcgggcggca gctatgtcct gctgcaaaaa tacctgcacg
    16381 atttgaaaaa atgggatgcc gtccccgtcg ccgaacagga ggcctcggtc ggacgcggca
    16441 aggaaaccga cgacgaattc ggccgcgatg tccgcctgcc cgattcgcac ctcggccgcg
    16501 tcaacctgaa agaaaacgga gtcggcctga aaatcgtccg ccgcagcctg cccttcggca
    16561 aaatcagcgg cgaacacggt ttgatgttta ccgcatactg ccgcacgctg cacaatatcg
    16621 aagcgcaatt attgagtatg ttcggcgata cggacggcaa aaccgacctg ctgctccgac
    16681 atctctccgc cgccgtttcg ggcggatact actacgcgcc gtctgtcgaa cgcctgcaaa
    16741 acctctaagc cggacgatgc cgtctgaaac catttttcag acggcatttt cccgttttac
    16801 cgtccaaccg ttttccgagc gcgcactatg tctgcaagca tccaagcatt cgacccgcta
    16861 accgtcccca tttccggcac caacctgatt gaggcctctg ccggcaccgg caaaacctac
    16921 ggcattgccg ccctgtttac acgcctgatc gtattagaac aaaaaaacgt cgaacgcgta
    16981 ttggtcgtaa ccttcaccaa agccgccacc gccgagctga aaacacgcct gcgcgggcgt
    17041 ttggacgatg tgttacaagt tttagaaagc aaaggaattg ccaaacttgg agacgacacg
    17101 ctttcagacg gcattgccgc ctactgcgcc gaacaccacg aaggcgacac cttcctgccc
    17161 gaactcttaa aacaggcttt gcaaaaagag ggccggacgc gtctgattgt ccgcctcaag
    17221 gccgccatcg ggcaattcga caacgccgcc atctatacca tccacggctt ctgccagcgc
    17281 atcctgcgcg actacgcctt cctgtgccaa gcaccgttcg atgtcgaaat gaccgaagaa
    17341 gacggcgacc gcctgcttat cccggcgcaa gatttttggc gggaacgcgt cagcaatgat
    17401 ccggtgcttg ccgcattggc gcttaaacgc aaagctgtgc cgcaaaccgt ccttgcccaa
    17461 atttcgcgct atctttcccg cccgtacctg aatttccgcc gtccgcaggc ggatttgaaa
    17521 caggcgcagc gcaacgccga aacctcatgg caaaccatct gccgcctgct gccagaactg
    17581 gaagccggct tttggcgcat tcatcccgac ctcaacggca acagttaccg caaaaacagc
    17641 ttcggcaacc tttttaaaga actggcacaa aaatccgccg ccggacaact gccctttctg
    17701 gacaaagaca cacacgacag actcttgaaa cttgcatccg acaaactcga agccggactg
    17761 aaaaaaggca aaacgcccga tgcggcagta tttgccgaat tgcagaaact ggcagacttc
    17821 gggcgcgatt tgaacgcact cgaagaagcg gaagaagcaa caatgatccg gctgcaactg
    17881 gatttaatcg aatacctcaa ccgcagcctt gccgagatga aaaaatcgcg ccgcgaacgc
    17941 ggtttcgacg acctgctgct cgatgtccac accgcgctga ccgacaatcc gcacgccgac
    18001 actctcgccc gcgccgttgc cgaaaactgg gaaaccgcgc tgatcgacga gttccaagac
    18061 accgacccgc tgcaatacga aatcttccaa aagattttca tcgcccaaaa ccgaccgctg
    18121 ttcctcgtcg gcgatcccaa acaggcgatt tacagctttc gcggagcgga catttacgcc
    18181 taccttcagg cggcggaaga cgcgcggcac cgctacacgc tcgccaccaa ctaccgcagc
    18241 cacgccgcgc ttatcggcag cataggcgcg ctgttccgcc tcaaagaacg cccgttcgtt
    18301 ttggaaaaca tcggctattc ggaagtcggt gcggcgcgtg ccgaaagcag gctgtccccc
    18361 gaacgtcctg ccgtacaggt tcgttggctg cacgaaaacg acaatgaaaa agccaacaaa
    18421 gacgttttgc gccgccgtgc cgccgactat tgcgccgacg aaatcgccca cgcgctcaac
    18481 gaagccgcca gaggccgtct gaatttcaaa ggctgcccgt tgcagtcggg cgatattgcc
    18541 gtgctggtac gcacgcacaa cgaggcggtg atggtttccg ccgccctgaa aaaacggcag
    18601 gtgcaaagcg tcctgctttc gcgcgaatct gtgttcgcct cgcccgaagc cgccgccctg
    18661 tccgcactca tcggcttctg gctcgaaccg cgccgcgccg gaacgctgcg ttttgtcctg
    18721 acaagcagca tattcggtta tgacgcgcag caattgcacg acttcaacca aaacgaaagc
    18781 gagattttgc attgggcgga atctgcccga accgcactcg acaactggaa taaatacggc
    18841 attttcgccg ctatgcagca attttcccaa acacacggca tcgaaacgcg cctcttaagc
    18901 cggaacaacg ggcgcagtct gaccaattat ttccaactgc tcgaactgct tgccgccgaa
    18961 gacgcgcaaa accgcaaccc cgccgcgctg cacaaatggc tgggcgacca aatcagcctt
    19021 gccgaaaaca acagcggcga caaccgcgcc atccgtctgg aaagcgacga agatttggtc
    19081 aaaatcgtta ccatgcacgc ctcgaaaggt ttgcagtatc cgctggtgta ctgcccgttt
    19141 gcgtgggacg cgcaagatac cggaccgtcc gactggcaaa tcctccacca aggcgcaaac
    19201 cgaaccgaac tgttggcaaa ggcgcaactg tcggaagacg aacagaaaca atacgccgat
    19261 gaagaaatgg cggaacgcct gcgcctgctt tatgtcgcgc tgacgcgtgc cgaggaacag
    19321 ctcaacatct acgccgcgta ctccaccaat actgccgaca accccctcgc ctacctgatt
    19381 gaaggctcgc cccaagacag ccgtgaaacc gtccgccgtg cctatgcgtg tgaaaaagac
    19441 ggcatcgcga tgctcaaacg caactggcgg cgcgtggcgg acaacgcccc ttccggcaca
    19501 aatttcgcct tcaccgaaaa tgcgccgccc cctgccgcct atcgcagcaa cgccggtcaa
    19561 accgccgaat ttgccgcaaa cagcattccc gaacgcggat tccgatttgt ccgccacact
    19621 agctttaccg ccttaagccg ccatacccaa acgcccgacg gcggcgaaga agatgcctgc
    19681 ccgtccttgg atgccgccga aacctcggtg ccggcgatgc cgtctgaaac gccgacggct
    19741 tcagacggca tatcgataca cgattttccg aaaggcacgc aggcggggct gtgcctgcac
    19801 gaaattcttg aagatttcaa attcggacaa gcagccgccg gacaggaaac cctcattgcc
    19861 gacaagctga aaaaatacgg ttttgaagaa atatggctgc ccgccgttgc cgaaatggcg
    19921 gaagcctgcc gcaaaacgcc gctgacgggg gcatacgacc tgtccgacat ctcacccgag
    19981 tgccgctgtc ccgaaatggg ctttaccctc cacaccgaag acttcagcct caaacgcctg
    20041 cgcgactggt ttgcccgcga cgacatcagg ctgcccgaag tctgccgtgc cgctgccgaa
    20101 acgctcgact tccacaccgt caacggcttt ttaaacggct ttgtcgatat ggtctgccaa
    20161 gaccccgacg gcaatatctg cgtcatcgac tacaaatcaa accacctcgg tacggacgca
    20221 tccgcctaca cgcaacaggc aatggacgaa gccgtcgcac accaccacta ttaccttcag
    20281 gcactgattt acgccgttgc cgccgcacgc tacttcaagc tgcgcggaca accgcccgcc
    20341 gccgtttccg tccgctacct gtttttgcgc ggattggatg gcaaaggcgg cggcgtttgg
    20401 cgttgggaca tagatgccgc cgctttggaa cagataaaat aacttcccgt cctttcggac
    20461 aacaaccgca ggaaaaccct atgaatatca aacacctgat taccgccgca ctcattgcct
    20521 cagccgcctt tgccgcgcag gcagccccgc aaaaacccgt atccgccgcc caaaccgcgc
    20581 aacatccggc cgtttggatc gatgtccgtt ccgaacagga atttagcgaa gggcatttgc
    20641 acaacgcggt caacatcccc gtcgaccaaa tcgtccgccg catacacgaa gccgcgcccg
    20701 acaaagacac gccggtcaac ctctactgcc gcagcggacg gcgtgccgaa gccgcccttc
    20761 aagagctgaa aaaagcaggt tatacaaatg ttgccaatca cggcggttat gaagacctgc
    20821 tcaaaaaagg gatgaaatga cgcaatgccg tctgaaagga caggctttca gacggcatac
    20881 ggcgttccgg cacgcggcgc aacatcgggc gcggcgcgcg ccgccccgtc cgaagcctga
    20941 taaacaaacc gataaggaac aatgatgttt cacgacacat aaagcaccgc cctatgtgtt
    21001 gccctgattt ggaaagggtt acacccctcc cgaataaagt ctgattctac cgtttcaacc
    21061 aaaaaggaaa aaggaaatac gatgaaacac atactccccc tgattgccgc atccgcactc
    21121 tgcatttcaa ccgcttcggc acatcctgcc agcgaaccgt ccacccaaaa cgaaaccgct
    21181 atgaccacgc ataccctcac ctcaaaatac agttttgacg aaaccgtcag ccgccttgaa
    21241 accgccataa aaagcaaagg gatggacatt tttgccgtca tcgaccatca ggaagccgcc
    21301 cgccgaaacg gcttaacgat gcagccggca aaagtcatcg tcttcggcac gcccaaagcc
    21361 ggcacgccgc tgatggtcaa agaccccgcc ttcgccctgc aactgcccct acgcgtcctc
    21421 gttaccgaaa cggacggcaa agtacgcgcc gcctataccg atacgcgcgc cctcatcgcc
    21481 ggcagccgca tcggtttcga cgaagtggca aacactttgg caaacgccga aaaactgata
    21541 caaaaaaccg taggcgaata agcccgtcgc cgacatatgg cggatggaaa tcaaatgcac
    21601 acgcccctca ttcccacgaa gtcgggaatc caatcggttg ggttaccgtg attttcaatc
    21661 attgccgaaa cggacgattc cggattccca cccttgcggg gatgacgggc gtgtgtgttt
    21721 ttgccacatc acaggcaact ttgccgcaaa tcttatctgt cgccggtatg ccttcttgtt
    21781 tcaatccgcc acaaaccaaa tgccgtctga aacccgattt cagacggcat ttccgtttat
    21841 tccctgctgt cgcggcaaat atcgatggct tcctgcaaac tcgaaacgcc gtagattttc
    21901 aggttcggaa actctttggc gttgcgcggc atattggctt tggggacgat ggcgcgtttg
    21961 aagccgagtt tttccgcttc tttgagccgc tcttgcccgc gtgcgacggg gcggacttcg
    22021 ccgcttaagc cgatttcgcc gaaaaccacg gttttttcag gcatagggcg gttgcggaag
    22081 ctggaaagca tcgcgaggat gaccgccaaa tccgccgccg gttcgccgat tttcacgccg
    22141 ccgacggcgt tgaggaacac atcctgatcg aaacaggcga tgccgccgtg gcggtttaac
    22201 acggcaagca gcatcgcaag acggttttgt tccagtccga cggtgaggcg tttgggcgtg
    22261 aagccgtgcg cgtcatcgac caatgcctga atttcgacca aaagcgggcg gctgccttcc
    22321 tgtgtaacca aaacgcacga gccgggcgta tcgtcgcggt agctggcgag gaagatggcg
    22381 gacgggttgg acacaccttt caaaccgttt tccgtcatcg cgaacacgcc cagttcgttt
    22441 gccgcgccga agcggttttt gatggcgcgt atcatgcggt agttggaatg ttggtcgccc
    22501 tcgaaataca gcacggtatc aaccatgtgt tccagcacgc gcgggccggc aatcgcgccg
    22561 tctttggtca cgtgtccgac cagtatcatg gcgatgccca tctgtttcgc catgcgcgtc
    22621 agttgggcgg cacactcgcg cacctgcgac acggagccgg gggcggacgt gatttggtcg
    22681 gaatacatgg tttggataga gtcgatgacg acaacttcgg gctgatgctg tttcaaggcc
    22741 gcctgaatcg cttccatgcg gatttcggca agaaggttta cgccgtcggt cggcagttcc
    22801 aaacgctgcg cgcgcagggc gacttgttgg gcggattctt cgccggaaac gtatagcact
    22861 ttacggcttt gcgccatttt ggcgatggtt tgcaacagca gcgtggattt gccgatgccg
    22921 gggtcgccgc cgagcaggat gaccgcacca tcgaccaaac cgccgcccaa tacgcggtcg
    22981 agttcgccca taccggtcgg attgcgcggc acttcggtgg cggtaacggc ggagagggat
    23041 tggacggtcg aggtatccgc cgcccaagat tggaaacggg cgtttttcgg ctcgggcgcg
    23101 gcaaggcttt cctgaagcgt gttccactcg ccgcaatgcg ggcatttgcc ctgccatttc
    23161 ggcgaagtgc cgccgcattc ggtgcattgg taaagggttt taagcgtttt tgccatcggt
    23221 tttccctgca tctgaaaaca aaaatgccgt ctgaacatag ggacggttgg gattgcgcca
    23281 atacgacaac gccgccgtca tgtactatcc gtacacggcg gatattgccg cattgcctta
    23341 tttttaccct aaccggccat ctctcggctt cagacggcat atcgggcggt ttcctgccgg
    23401 cttacccgcc gtgatactgc cgagacagct cgtgtacggt atcgactaag attttggcgt
    23461 gttcggggtc ggcgtgttgg ttgatgccgt gtccgaggtt gaagacatgg ccgctgccgt
    23521 gtccgtagtc ggctaggata cgtgcgacct cggtgcggat ggattccggc gtaccgaaga
    23581 gggcgaacgg gtcgaagttg ccttgcaggg cgacttgctt gccgacgcgg cggcgtgctt
    23641 cgccgatgtt acacgtccag tccaagccca atgcgtctgc gccgatttgg gccatacttt
    23701 ccagccacag cccgccgcct ttggcaaata cgataacagg cacgcggcgg ccttcgcttt
    23761 cgcgtttgag tccggcgacg atctggcgga tgtatttgag gctgaattct ttaaacgccg
    23821 catcgctcaa cacgccgccc caagtgtcga aaatctgcac cgcctgcgcg cccgcgtcga
    23881 tttgggcgtt gaggtaggcg gtaacggctt gggcgttggt atcgaggatt ttgtgcagca
    23941 aatcggggcg cgagtacatc atggttttga tggtgcggaa ttctttgctg ccgccgcctt
    24001 cgaccatata acaggcgagc gtgaacggac tgccggagaa gccgatgagc ggtacgcggc
    24061 cgtccaatgc tttacggatg gaagttaccg cgtcgaaaac gtattgcagt ttttccatat
    24121 cgggaacgtg cagcttggcg atgtcggcct cgtgttgcag ggcgcgtttg aatttcgggc
    24181 cttcgccttc ggcaaaatac agtcccaagc ccattgcgtc agggacggtc aggatgtcgg
    24241 aaaacaaaat cgccgcgtcc aaatcgaaac gttccaaagg ttggatggta acttcggtcg
    24301 ccaattcggt gtttttgcac aaatcgagga agctgcccgc tttcgcgcgt gtggctttgt
    24361 attcgggcag ataacgcccc gcctggcgca tcatccaaat cggcgtgtat tcgacaggtt
    24421 gtttgagcag ggcgcggagg aaagtgtcgt ttttcaaaga gatcatgtgg ggctttcggt
    24481 ttgtttgtaa aacggggaag gaaaacggtt ttcagacaac ctttgcggcg gtttccgcaa
    24541 tgaaaggtcg tctgaaaagg gagggacggg cacatttgcc taagtaatca tcagtagatg
    24601 attaaacggc agcaggtttt catggggttt caatggtgcg tttatcttcg atatggataa
    24661 ctttgggttc ttcagcatca caatcgcgat gagagcgccc ttcgataccg attttgaaat
    24721 cttcatccaa tccttcttct tttaggatta gcgtaaaaat ttcgggatga tggcatgaaa
    24781 cggcatatcc atcgccacta tctaaagtga taatgccaaa atctgcagat ggtgttccta
    24841 tttgaacctt gccccatttg cttaacatct ctttagcttg tttcgccaaa tctttttccg
    24901 gttcaagtaa aatgacgcaa gtcccatttt caaagagcac ccaagatttt tcgtttccat
    24961 taatgctctt tttccaaaca tcaattaact tttggcgata agcaggcgtt ccaatttcaa
    25021 atatttcttt cttttcctta aaggaatctg aagaccccat caaattctcc cagataaaga
    25081 tatttcccaa aatcagctat gctgctctaa cgctgcgtga cgttcgtcat cggagacggc
    25141 ttccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccgattt cgtcgaaaac
    25201 ctttgctaga accaaacgcg cggaaatact cggctttaat gcaatgctcg cttcaaggta
    25261 gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat acatcagcag
    25321 aagcgcgtta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg ctttctgctg
    25381 ttcgcgctcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagct cggggcggcg
    25441 gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc ccaaacgttc
    25501 gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg gaatccgctt
    25561 caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc ggtatgccca
    25621 attttgatac cgttccattt ccgatttgcc caacgcgccc gccttggaaa gtttttcggt
    25681 ttttgccaga acctgcaacg cgtcgcccct gtcgaaagcg taacgaagtt gcagacgcac
    25741 gaggcgcgta aggttggcat tcatcttcgc cgccgcatga agattggctt ccgccgcttc
    25801 gtaatcgcgc cggttcaacg ccgattccgc caacaaaaga taacgggaaa gctgctgttt
    25861 ttccggcagt ttggcgattt ccgcaagata acggtcgcgc agctcgatgt tttccatctg
    25921 tccggcggcg tgcgcgccca gcatcaatgc caaagtccgg ttgtctccgg cctctttgtt
    25981 gaccaacacg cgtgaggctt ctagttccgc cttttcaaaa cgcccttcaa aatacgccaa
    26041 acccgccttg ttcaaggcaa gcgcggcctt gcggccttta cgcgccgaac cgaaacgctg
    26101 catcttttcg gggatattga gtacgccgat aatgaattta aacaagaaat accacaccac
    26161 gacggcaatc agcgaaccta acacaaaggc gtgcaggttg attctgagca tggtctgtcc
    26221 gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc agcgccagtc cgacggcggc
    26281 ggcaaacagg acgacaatcc agactaccgt tttcatgcgc gttctccttt agcggacggc
    26341 gaacgccctg ccttttccgg tacggtctgt ttttttgcag gctgttccgg tttgcgttcc
    26401 gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccgg ttcggaagct
    26461 gttttttcgt ttgccggttc ggaagccgcc tgttccgctt cttgattttc cgccgccgtc
    26521 atgcgcgtac cgtcgcgata ggcgcggacg gcatttaggc tgtttttcaa accgtcatcc
    26581 gcagtcatcc gcacatccaa cgccttcaat tccgccagtt ctttcagcca cgactgcgtg
    26641 gcgggagact tggcatcgaa atactgtctg acggcggctt cggcattgtt caaatcgccc
    26701 tgatagactt cgctgttgcg ctgcattaat gcagtgcgcg catccaaaag gcggaggcgc
    26761 aggttttcac gcacaaaata tgcctgttcg ggagaaatca gcatggcatc gttgttttcc
    26821 aaacgtcgga tttcgaccag ccccttcaat gtgccgaggg atttttccca tacgttctgc
    26881 caccatgaag cggaagcggc ttcgttcttc acctgtacgc ccggtttcag cacgccgtcg
    26941 agcatcagcg gcagtccgga tacggcggtt tccagcctgt cgaggcgcaa tgccgtgccg
    27001 gaaatatcga catagggacg gtttttcagt tccgccaagt cgctgctgac cgcctgcttg
    27061 atcggcagaa gctctgcctg attgaaacgg gacaggcggc tgtcgatatg ctccaatacg
    27121 ccgactgccg tttggatatt gccagtcaac accagctgtt gcgccgccag attgagtatg
    27181 gtctcggttt cgtccaccag ccaatcggcg cgtcctttgg tcaactcgcg ataggatttt
    27241 tgcatttcca agatttgttc gccgtttgct ttgacgtttc cgtccaaacg gtcgagctct
    27301 gattgtatgg cggcttgccg gttgaggttg tctttcaaca gggcggcgtt ttccgactcg
    27361 cccaaggcgg ctttgtcgat tttttggttg aatgccagct cttggttttt caagacattc
    27421 tgtccttgga caaacaaaaa accacttgta cccaaaccca atgccgccaa taccagggcg
    27481 cagactgcca aagcgttgct gccggactgt ttgacgataa aaggagcggc aggaatgtgt
    27541 acttctgttt cgttttcttt gcgtggggaa gaggtttcag acggcatttc ttttgatgcc
    27601 tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt cgcccaccgt tttgctcctt
    27661 attaatttga ggttccggga aagaccatgc cgtctgaaac ggaaatggaa gaatgggaaa
    27721 gcgcggcttc cagcgtaggg acggtttcga ccgaacacac gccttcgcgc ttcaatgcct
    27781 ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt gaagaatcgg gaaaattgcg
    27841 gcggaagctg cccgaacagc aagcgcacca gctcggtcga cgtaatatag gcggcggcaa
    27901 tattttcggt ttggaaattt tgaaagttca aaggtttatg ccgtctgaaa tagacttctg
    27961 ccacctccgt ccgaaaacct ttctcctgca aggcattcat caaaaaatcc cgcccgccgt
    28021 gtccgcgcac aaacaatacg cgcgcacctt cgggcagact gttccaaacc ggcaggcgca
    28081 aaaccgcctc gctgtcgttg ccgtcatcag gcgcgatgac cgttctgacc aaacagcgtt
    28141 ccaatgcgcg gcggctgccc tgccctacgg caatctgcgc ctttatgccg tctgaaaggt
    28201 taaggtacgg gacggcggtt tcaacggcgg tcggactgac ccaaaacacg gcatccgcac
    28261 gggcatattg ttcggacaaa agttccagtc cggcagcatc tgcttcgatt tcgaccggac
    28321 tcaatacttc cgcgcgccaa ccggcattca ggcaagtttc gacatcttct gcggccctgc
    28381 ccgacggacg gacaatcagc atgacgggtt tcgctgtttc catcgcctta tccgccccga
    28441 ttactaggaa aaatcgtcat cgttgaaatt cgcctgcgcg gttttcggcg tacagccgac
    28501 ccgtgcgact tccttgcccc tacgctccac aacgacaccg ctgctttccg tatgagtcac
    28561 ggaatcgaag cctgtccata tggtgtacat aaattcgccg ttttggaatt tcatcgttgc
    28621 ccaacgaccg ctgcccatac cttgccacct gtcggaacgc cccaacaggt cagctttgct
    28681 gttgcgtatg gcaatttctt tttttgccgc actgccgaac gaatattcgt aaagattgcg
    28741 gttgattttt tggacttcta tgtatttgtt gttgtccgtt ttacaggaaa acaccgtatc
    28801 cgcccacacg gtttgggcgg cagccgacag aagcaatacg gcagaaagga acattttttt
    28861 catcgtattt ccttaatggt tgaaaccccg ccactcggac atccgtcctt cgtggcagca
    28921 gcatcagatt ttatttgaaa agggtgtaac cccttccaaa ttagggcaac acatagggcg
    28981 gcgctttatg tgtcgtcctg tgtgttgaaa catcattgcc cgtatcctga aatagaaaag
    29041 aaaatgcccg ttttacggtc ttctttccga taaaatgccg tctgaaacgg atttcagacg
    29101 gcatggtcga aaattattgc gcggcaagcg gcttgagcag caaggtttcg ccgctttgca
    29161 cgacattttc aagcaggtcg atattgggat gcttgccggg atgggcgcgc gcgtcggcaa
    29221 cgtgttcggc aaaccatatc aaaccctgtt ctgccgcttc tttgtccaat tttccgtcaa
    29281 aacgttctgc caaagcattg tacagcttga gcgaaccgag cttaccctga acggcgggaa
    29341 tgtggtgaac cgttttgccg tcggcatcct gaacgtccaa accgcccaga tggtcgattg
    29401 aaggaaatgt ggcgagatag tcttggaaat tcatatcaaa tccgaaaatc agtcagttag
    29461 cgcgtgccga aaatcttgtc gcccgcatcg cccaagccgg ggatgatgta gccgttctcg
    29521 ttcaagtggc tgtcgagcgc ggcggtgtaa atcgtaacgt cgggatgcgc gtcgttgacc
    29581 gccttcacac cctcgggcgc ggcaaccagc accagtgcct tgatgttttt gcagcccttg
    29641 gctttcaaaa ggtcgatggt ggcaaccatc gaaccgcctg tcgccagcat aggatcgata
    29701 atcaaagccg gacgttcgtc catactgtcc acaaatttct caaaatagga aataggcttc
    29761 agcgtttctt cgtcgcgctg cagtccgact acactgattt tggcagtcgg aatcaggtcg
    29821 agcacaccgt caagcatacc caaacctgca cgcagtatgg gaacgacggt caatgttttg
    29881 cccttgatgc ggtcgccttc aatctgaccg caccatccgt cgataaggta tttttcgatt
    29941 tcaaaatcac ggcttgcctc gtatgccatc aggcgcgcca gctcggtggc aagcgtccgg
    30001 aatttgtagg tgctgcaatc cgcctccctc atcagggtta atttgtggcg gacgagcgga
    30061 tggttgataa cattaacgtt catcgcggtg ttccaatgtg attttgagac gcgccattat
    30121 actttttttt gatttgccgg aaaagaaaat gccgtctgaa acacgttcag acggcattgc
    30181 cgaatccatc aggctttcag cagctcttgc agctcgcctg cctcatacat ttccatcagg
    30241 atgtccgaac cgccgacaaa ctcgccgttc acataaagtt gggggatggt cggccagtcg
    30301 ctgtattcct taatgccttg gcgcacttca ggattttcca atacgttgac ggtaacgtaa
    30361 tcggtgcagc ctgccgcgtt caggatttgc acggcgcggg aagagaaacc gcattgcgga
    30421 aactgcttcg tacctttcat aaacaatacg acgcggtgtg tcgttactac ttctttaatt
    30481 tggtcgtgga tggaagccat cgcttattcc ttttgataaa gttgacaaaa tttgtcaggt
    30541 agggcgcatt atacgcaaaa gcggaaactt cccacaatat ttgccgtata tcgcgcagta
    30601 tcaatattgt gaacggaaat attcccactc ctcgacaatc ctgctgtccg gcaaatggca
    30661 cagggcatat ccgccgccgc tttcgtcttt ttttgcttcc cgtctgccgc ctttggcaat
    30721 gcagaatttg gaagcggaat ttgccatgcc tgtccgaaac gtgttggcag gcagccggca
    30781 gcagcgcgga catgcatacg gatgaattgc cttcatggtt ttccttatcg tcggtacagg
    30841 gtcagacggt ccgcatatcg ggtacttttt taaacagccg gtaaaagttt tccgtcgtat
    30901 atgccgcaac ctgttccaat gtttggttcc gcaatttggc gatatgttcg gcggtatggc
    30961 gcacaaaagc cggttcgttc tgcctgccgc gtttgggaac gggggcgagg aacggcgcat
    31021 cggtttccac caaaatgcgg tcgtccggca catatttcgc cgcctcctga accaagggtg
    31081 cgtttttaaa ggtaacgatt cccgagaaag aaatataaag ccccaaatcc attgctgcac
    31141 gggcaaaacc gatgtcttcg gaaaaacagt ggataacgcc cgaattaacc cggcattctt
    31201 tcaggataga caaggtgtcc gccgccgcat cacgcgtatg gacgataacg ggcagtccgg
    31261 tttgattggc tgcttcgatg tggtctgcaa agcgtttgtg ttgccaggac aaatcgcctt
    31321 tgcaccagta ataatccaaa cccgtctcgc cgatgccgac cactttcgga tgggcggcgg
    31381 cttcgaccat ttccgcaatg gaaaattctt cggcttcctt gctgtcggga tgtacgccta
    31441 tggtgcaata gatgtgttcg tgcgcttcgg cgatggcaaa gacttcggag aagctttccc
    31501 tactgacgct gatggcgagt gcctgcccca cgccgtttgc ttccatgttg gacaaaactt
    31561 cgggcaggcg ttctttcaaa ccttcaaaat tgaggtggca gtgcgaatcg atgatatgca
    31621 tggcgttaca tggtaaacgt aggtctgctg ccgccgattg tgttgcccag ttcgacttcg
    31681 atctggtctt tgactttcag gcagttttcg tgttttgtga atgccagccc cacccctttg
    31741 ggtttggagg aagtacgcgc aggattgatc caggcgactt tggtcggcag gaacagtttg
    31801 gggaagttga ggatttctac ggcaagcaga atatcgtccc cgatggaaaa tacgtcgttg
    31861 gtctgcacaa acagaccgcc gtgttccaaa aacggcatgt aggagctgta cagcagattc
    31921 atgtctttca gctgcaacga catcattttt gccggaatat tttgtccgtc tgacataatt
    31981 cacccgttct ctttcttcaa ttccaaatag ttgatgagca gatgctcgat ctgcattttg
    32041 acatttaaag tatgaaaccc gtagggggca agctgtttga gcatatcctc cgccgcaaat
    32101 acattgcgcg gacggaaacc ggataccgtc tgaagcagcc tgtcttcata agcgggataa
    32161 tagacgggtt tcatgtgttg caggcacaat cccaaatcga ccagccattt ctgcatccac
    32221 ccgacaaata cggcgagcgg aagtttttcc ttatcgaaaa gcgcggcgta atccaaaatc
    32281 ttcaacaacc ttggttctgc caaaatatcc aacagtttaa tccgcaacgc acggacaccg
    32341 tccgcctcat caaacagcgg cgctccggaa tggaaagcca gacgttcctc aggttccgcc
    32401 acaccccttt cacgcagata tgccaatgcc tcttcatggg aaggagcggg caaaaccatc
    32461 ttccggcagc gacttttaat ggtcggtaaa accttgtccg ccgcgtggct gaccagcaaa
    32521 aagaccactt gtggcggcgg ttcttccaac actttcaaca aactgttggc ggcttggaca
    32581 ttcatacttt ccgcaggatg aatcagaatc acgcgcaaac cgccccgtac cgaagtcagg
    32641 tacacattat cgatgatttc cctgacggca tcgattttga tctgcaacag tttgcgtccg
    32701 ttttcgggtt cgtccgacaa gggggtgatt tcgtaaaaat cgggatggct tccctgtcca
    32761 aacagatggc aggacataca ttcgccacag ggtttgcagc ccggtgcagg ggtttcgcac
    32821 aacagtgcct tcgccgcaaa gcgggcaaat gtagttttcc ccgtatcttt tttgccggca
    32881 aacagccatg cattgggacg acgctcccaa tgttccgcaa tctgccgcca ttgctcatta
    32941 tgccacggat aaatcatttc aaaccatccg tccggaaatc cataccgcca tcagagcagt
    33001 tccaaatcgg ggctggaact ttgcgcgcgg cggctgcgca actgtaccgc caaacgcaaa
    33061 tcgtgtgcgg aatcggcatt tttcaatgct tcttgcaggg aaatatcgcc tttttcatac
    33121 aattggtaaa ggtgttgatc gaaggtctgc atacccaggg tagtggattt tttcatcact
    33181 tctttgattt catggatgtt gccgttgtga atcaactccg aaatcagggg cgaattgagc
    33241 agcacctcga ctgccgccac cctgcccttg ccgccgtctc gcggaacgag gcgttgcgaa
    33301 ataaacgcct gaaggttgag cgacaaatcc gtcagcaatt gttcgcgccg ctcctcgggg
    33361 aaaaagttga tgatgcggtc gagtgcctga ttggtgctgt tggcgtgcag cgtcgccata
    33421 cacaaatgcc ccgtttcggc aaaggcaatg gcgtagtcca ttgtttcgcg gtcacggatt
    33481 tcgccgataa ggatgacatc aggcgcctga cgcagcgtgt ttttcaacgc cgccatccag
    33541 ttttccgtat ccacgccgac ctcgcgctgg gtgatgatgc agtttttgtg ttcgtggaca
    33601 aactcgatcg ggtcttcgat ggtgatgatg tgtccgaacg aattttcatt gcggtagtcg
    33661 ataagcgagg caagcgaagt cgatttaccc gagccggtgc cgccgacaaa aataaccagc
    33721 ccgcgttttt tcagcgcgac atccttcaag actggcggca ggttcaggct ttcaaacttg
    33781 ggaatcttgc tggtaatcgt acggaatacc aacgccgtcg cgccgcgctg tatcatcgca
    33841 ttgacgcgga agcggctggt gtccggcagg ctgatggcga agttgcactc gttggtcgat
    33901 gaaaattctt ccgcctgctt cgcactcata atcgaaaagg cgatttccat acatttttcc
    33961 gccgtcagcg gttcgtccgt gatgcgggtg attttgccgt ccagcttcat tgcgggcggg
    34021 aaatgggttg tcacgaacag gtcggaacct ttgttttggt tcatatggcg cagccatgcg
    34081 aacagttctt cctttgcggt catattttca tcggtaaaca tcctcgtccc ctaatcttca
    34141 aacaatctgt accttcccat tctaccaaaa acccgccgtg ccgtctgaaa ccgtttcaga
    34201 cggcacggac aaaccacctc cactcaatcc tgccgtgtta gcgtgtataa tcaggcattc
    34261 tatccgaaat tcacaacagg tcagcaatat gtctttgaaa acagtagccg ttatcggcgc
    34321 aatggaacaa gaaatcgagc ttttgcgcga gatgatggaa aatgtcaaag ccgtctcttt
    34381 cggcagattt tccgcctatg aaggcgaatt ggcgggaaaa cgcatggtgc ttgcattgag
    34441 cggcatcggc aaggtcaacg cggcggttgc aacggcttgg attatccgtg aattcgcagc
    34501 ggactgcgtc atcaacaccg gcagcgcggg cggtttgggc aagggtttga aagtcggcga
    34561 cgtagtcatc ggcacggaaa ccgcacacca cgatgtcgac gtaaccgcat tcggctatgc
    34621 ctggggacaa gtgccgcaac tgccggcaag gtttgcttca gacggcattt tgattgaggc
    34681 ggcaaaacgg gcggcgcgga cgtttgaagg cgcggcggta gaacaagggc tgattgtcag
    34741 cggcgaccgc tttgtccaca gcagcgaagg cgtggcggaa atccgcaagc acttccccga
    34801 agtcaaagca gtggaaatgg aagcggcggc aatcgcccaa acctgtcatc agttggaaac
    34861 gcctttcgtc atcatccgcg cggtttccga ttcggcagat gaaaaagcag acatcagctt
    34921 tgacgagttc ttgaaaacgg cggcggcaaa ttccgcaaaa atggtggcag aaatcgtcaa
    34981 atctttataa gacctttatc aaacttatgc caaaacagcg gttttccgtt agaataacgg
    35041 gctatttcca cgttgcaccc gatattcgga cgcaacgccg tttgttcaga cggcatttcc
    35101 caaacccact gtccgacatt caaaacaact ctaatgaaaa atatccgaaa tttctccatc
    35161 attgcccaca tcgaccacgg caaatcgacg cttgccgacc gcttcatcca atactgcggc
    35221 ggtttggatt tgcgcgaaat gagtacgcag gtgctcgatt ccatggacat cgaaaaagag
    35281 cgcggcatta ccatcaaagc gcaaaccgcc gcgctcaact ataaagcacg cgacgggcag
    35341 gtgtatcaac tcaacctgat tgacacgccg ggacacgtcg acttctctta cgaagtttcc
    35401 cgttcgctgt cggcttgcga aggcgcgctt ttggtcgttg acgcgtcgca aggcgtggaa
    35461 gcgcaaaccg tggcgaactg ctacaccgcg attgatttgg gcgtggaagt cgtgccggtt
    35521 ttgaacaaaa tcgacctgcc cgccgccgac cccgaacgcg tggaacagga aatcgaagac
    35581 atcatcggca tcgatgccgt cggcgcggtg caatgttccg ccaaaagcgg catcggcgtg
    35641 gaagacgttt tggaagaaat cgttgccaaa atccccgcac cgaccggcga cgaaaacgcg
    35701 ccgctgcaag cggttatcgt cgattcgtgg tttgacaact atgtcggcgt ggttatgctg
    35761 attcgagtga aaaacggcac catcaaactg aaagacaaag tgcgctttat gagcaccaag
    35821 gcggaaacgc aggtcgagca gctgggcgta ttcacaccga aatcagttca aaaacaagaa
    35881 ctaaaagccg gcgaagtggg ctttttgatt accggcgtaa aagaattggg acaggcgaaa
    35941 gtcggcgata cggttacttt ggttgccaac cctgccactg agccgctgcc cggtttccaa
    36001 gaggtgcaaa gccaggtatt cgccggactt tatcccgtag aaagccacga ctacgaagct
    36061 ttgcgcgatg ctttggaaaa attgcagctt aacgatgctt cgttgaaatt tgagcctgag
    36121 gtttcccaag cattgggttt cggcttccgt tgcggcttct tgggtctgtt gcacttggaa
    36181 atcgtgcagg aacgcttgga gcgcgagttc gatatggatt tgattaccac cgcgccgacg
    36241 gtgatttatg aagtcgtgtt gaaaagcggc gaaaaaatcg aagtcgaaaa tccgtccaaa
    36301 ctgcccgaca tcggcagcat cgaaaccata ctcgagccga ttattaccgc gaccatcctt
    36361 gtgccgcagg aatacgtcgg caacgtcatg actttgtgta accaaaagcg cggcgtgcag
    36421 gtcaatatgc agtacatggg ccgccaagtg atgctgactt acgatttgcc catgaacgaa
    36481 gtggtgatgg actttttcga caaactcaaa tccacttcgc gcggctatgc ttcgttggat
    36541 tatcatttca aagagttcca accgtctgat ttgattaagc tggatattat ggtcaacggc
    36601 gaaaaagtcg atgccttaag cctgattgtg caccgtcaaa gcgcggttca ccgaggccgc
    36661 gagctagcat cgaaaatgcg cgagctgatt ccgcgccaaa tgttcgacat tgccgtccaa
    36721 gccgccatcg gcagccagat tatcgcccgc gaaaacgtca aagcactgcg taaaaacgtc
    36781 ttggcgaaat gttacggcgg cgatattacg cgtaagaaaa aactgcttga aaaacaaaag
    36841 gcaggtaaac gccgtatgaa acaagtgggc aatgtggaaa ttccgcaaag cgcgttcttg
    36901 gcgattttgc aggtgagcga caaataactc cggcacacag ggcggcgcgt cgggaacacc
    36961 cctcaaaccg ccctgatttc aggggcacgc gcccgcaaac aaattttgca atttgccgcc
    37021 ttaaaacggt tgcacggaca ggcgggtttt taagcattaa ggaacaacaa tgaacacaat
    37081 gctaatgtcg ggcgcggctg ccgcgctgct tgccggcatc atcctttatt tcaaaagcga
    37141 caagaagcgg caggaaaacg gggaatggag ttccggcctt gaatacgcct atatcctgac
    37201 agcggtcggc gtgtttgccg ctttgtccct gtttatgagc tttaccgccg ttttcctgat
    37261 tttcgttgta ttgtgcggta cggcttgggg ggtatataaa taccgcctga agactcatcc
    37321 cgaaatctcg gaaagcagcc acttcggcga ttatttcggc agtttcttcc ctaccgtttt
    37381 ggtattgttc ctcatccggt cgtttatcgc cgaaccgttc caaatcccgt ccagctcgat
    37441 gcgcccgggc ctgatcaagg gcgatttcat tttggtcggc aaattttcct acggcctgcg
    37501 cgtacccgtt ttaaacaata tatttattcc tacaggcaaa atcgaacggg gcgatgtcgt
    37561 tgtttttaat tatcctctgc agccggagat gacctacatc aagcgtattg tcggcattcc
    37621 gggcgatgtg gtcgaatatc gggataagat tttgacggta aatggcaaac ccacttccga
    37681 cattcctgac ggcacatacc gttatcccga cgacaccgac ccttccgaaa tccacaacac
    37741 ggatatgttc cgcagcggtc tagacggcaa atccttcaat attctgaaaa aagaaggaca
    37801 gcctgccgtt tccctgcccg tattgggcaa atatacctcc gatattatgt ctgaaaacgg
    37861 atattccata gagcaaagcg gtttggaaca ctgccaatat gccgacgacg gcagcggttt
    37921 cgtgtgcaaa gttcccgaag gacgctattt cgctatgggc gacaaccgcg acaacagtgc
    37981 cgattcgcgc tactggggat ttgtggatga caagctggtt gtcggcaagg caatgttcat
    38041 tttgatgaac ttcggcgatt tcggcaggtc cggtacggca atccgttagg ccaggaagcc
    38101 aagaatgccg tctgaaaata gtttcagacg gcatttgcta ttcctcactt ttacaggcga
    38161 gtctaacgtt gtcggatttt cctacctatc agtccaatat acgcaggttt tgcacggcga
    38221 actgccaata ttgccctgat gaaatcaatg tgaagactgc cgtttcagcg aaccgaggta
    38281 aattgccgaa agcgtcagcg ccacccctgc ccactgcacg ccttcaatcg gtttgcccaa
    38341 cccgaaataa tcgatgaaca gggcggcaac cggttcggac aaaagcagca gccccgtcag
    38401 cgacagcgaa agcagcggaa tcgcataggc aaccatcgcc cacgcgaagc actgcatcac
    38461 cacgccgtac acaagcacca aacccgcatc tttccaagtc gtcggataaa gcgcgccgcc
    38521 atccatcagc aatgccggaa caaccagcga taccgcgccg cccaaactca aaatcatcat
    38581 tgaagggaaa agcgccaccg gctcgatttc atgggttttg cggacaaaca ccatcgacag
    38641 ggcgagcatc agtcccgata ccaaaccgct ggcgaatccc caaaccgcat taccgttgta
    38701 gccgaattcc gcaccggcaa tcatcgccac gccggcaact gccgatatta agcctgcctt
    38761 tttcagcccg ctcaaacgct cgccgaaaaa gaaaacaccg attgccgaca agaaaaagat
    38821 ttgcaggctg ttgagcaggg tggaaatacc cggcccgacc gcgtgtatgc tttcgtgcca
    38881 caacgccaaa tcgaaagcga gaaacacgcc cgccgtcagg gcatatcgga cggttttcct
    38941 gttttttggg aatttttgcc tgaaaaaccg tgctaaaaac caaaatacga acaccgaaat
    39001 cagcaaccgc caaaatgcga ttgcatacga accgacgggg acggatctga caatcagact
    39061 gcccagaccg aacaccacgc aacccagcac cagcaatggt gcggcatatt ttttgccgtt
    39121 accgaccaca atatcccctc ccttccccat acggctgccg cgccgacggc aagaatgccg
    39181 ccattccgaa aatatgccgt ctgaagccca aagccccggc tgcacccgga caaacggctg
    39241 attttacggg caggcaggct attttccgtt ggggcaaaaa accgtcctgc caaataaaac
    39301 cc
//
LOCUS       DAWISC010000003        31903 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWISC010000003 DAWISC010000000
VERSION     DAWISC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.003"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(177..824)
                     /locus_tag="WIW87_000089"
     CDS             complement(177..824)
                     /locus_tag="WIW87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ5173314.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(821..1189)
                     /locus_tag="WIW87_000090"
     CDS             complement(821..1189)
                     /locus_tag="WIW87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ5173315.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1199..1870)
                     /locus_tag="WIW87_000091"
     CDS             complement(1199..1870)
                     /locus_tag="WIW87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ5173316.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2035..2754)
                     /locus_tag="WIW87_000092"
     CDS             complement(2035..2754)
                     /locus_tag="WIW87_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5173317.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2837..4150)
                     /locus_tag="WIW87_000093"
     CDS             complement(2837..4150)
                     /locus_tag="WIW87_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ5173318.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WIW87_000094"
     CDS             complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WIW87_000094"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ5173319.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5591..7453
                     /gene="hscA"
                     /locus_tag="WIW87_000095"
     CDS             5591..7453
                     /gene="hscA"
                     /locus_tag="WIW87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ5173320.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7530..8186
                     /locus_tag="WIW87_000096"
     CDS             7530..8186
                     /locus_tag="WIW87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5173321.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ
                     "
     gene            8223..8984
                     /locus_tag="WIW87_000097"
     CDS             8223..8984
                     /locus_tag="WIW87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ5173322.1"
                     /translation="MMGDSVIYYVEQADEPVNRADERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDYLREQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASTTSEGYTALDFACLTGWQNVADLLEPRH"
     gene            9039..9380
                     /gene="fdx"
                     /locus_tag="WIW87_000098"
     CDS             9039..9380
                     /gene="fdx"
                     /locus_tag="WIW87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ5173323.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9803..10357)
                     /locus_tag="WIW87_000099"
     CDS             complement(9803..10357)
                     /locus_tag="WIW87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ5173324.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10570..10767
                     /gene="iscX"
                     /locus_tag="WIW87_000100"
     CDS             10570..10767
                     /gene="iscX"
                     /locus_tag="WIW87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ5173325.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10835..11236)
                     /locus_tag="WIW87_000101"
     CDS             complement(10835..11236)
                     /locus_tag="WIW87_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ5173326.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11448..12407
                     /locus_tag="WIW87_000102"
     CDS             11448..12407
                     /locus_tag="WIW87_000102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ5173327.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12475..13785
                     /gene="tilS"
                     /locus_tag="WIW87_000103"
     CDS             12475..13785
                     /gene="tilS"
                     /locus_tag="WIW87_000103"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ5173328.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAMGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13859..14644
                     /locus_tag="WIW87_000104"
     CDS             13859..14644
                     /locus_tag="WIW87_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ5173329.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14654..14794
                     /locus_tag="WIW87_000105"
     CDS             14654..14794
                     /locus_tag="WIW87_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173330.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            14779..14931
                     /locus_tag="WIW87_000106"
     CDS             14779..14931
                     /locus_tag="WIW87_000106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173331.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            15121..15465
                     /locus_tag="WIW87_000107"
     CDS             15121..15465
                     /locus_tag="WIW87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173332.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLSCYY
                     DEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            15477..15782
                     /locus_tag="WIW87_000108"
     CDS             15477..15782
                     /locus_tag="WIW87_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173333.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            15844..17220
                     /gene="mpl"
                     /locus_tag="WIW87_000109"
     CDS             15844..17220
                     /gene="mpl"
                     /locus_tag="WIW87_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ5173334.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            17427..18479
                     /gene="bioB"
                     /locus_tag="WIW87_000110"
     CDS             17427..18479
                     /gene="bioB"
                     /locus_tag="WIW87_000110"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ5173335.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18557..19192)
                     /locus_tag="WIW87_000111"
     CDS             complement(18557..19192)
                     /locus_tag="WIW87_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ5173336.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19515..19775
                     /locus_tag="WIW87_000112"
     CDS             19515..19775
                     /locus_tag="WIW87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ5173337.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WIW87_000113"
     CDS             complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WIW87_000113"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ5173338.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WIW87_000114"
     CDS             complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WIW87_000114"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ5173339.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23666..25480)
                     /locus_tag="WIW87_000115"
     CDS             complement(23666..25480)
                     /locus_tag="WIW87_000115"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ5173340.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25477..26763)
                     /locus_tag="WIW87_000116"
     CDS             complement(25477..26763)
                     /locus_tag="WIW87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ5173341.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPMSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WIW87_000117"
     CDS             complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WIW87_000117"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ5173342.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27763..28503)
                     /locus_tag="WIW87_000118"
     CDS             complement(27763..28503)
                     /locus_tag="WIW87_000118"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ5173343.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKTEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WIW87_000119"
                     /pseudo
     CDS             complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WIW87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
     gene            complement(30091..30249)
                     /locus_tag="WIW87_000120"
     CDS             complement(30091..30249)
                     /locus_tag="WIW87_000120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173344.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            complement(30262..30918)
                     /locus_tag="WIW87_000121"
     CDS             complement(30262..30918)
                     /locus_tag="WIW87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ5173345.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            30982..31872
                     /locus_tag="WIW87_000122"
     CDS             30982..31872
                     /locus_tag="WIW87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ5173346.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT         6878 a         8381 c         9258 g         7386 t
ORIGIN      
        1 acaaccacgc cggaattaag tttaaatttg aataaaaggt tcgggttctg caaaatacag
       61 aacccgaacc ttgttcggat attgaaaccg gctgcccgat tttgggcggt gcggcttgca
      121 agtatcaaga ttcgcatatg ccgtctgaag ctcggagagg ttcagacggc atatgcttat
      181 ttgggctgct cttcaacgaa tctcggacct ttcaagatgc cgttgtgaga atagggcgac
      241 agcaggttgt atgcggcggt tttggaaacc tgataaccgc ggtcggtcag gctgttggca
      301 atctgattga ccactgcgct gaccaaagcc cccaacaggc cgctgttgct gttgttgctg
      361 ccttcgcgga tgctggccga acccgaccac aactcttttc cgttgcggga atcgaccagc
      421 cgtgctttgg cggatacggt cgtcacgctg tctaaaattt gatatgaagt gccgtattcg
      481 gtaaccgtaa tgtacaaaac cgcatcattg ccgaaaatct gatgcagttt ttccggccgg
      541 acggcgtgaa tatcggcggc attggtcaag ccgttttgtt tgaaggtttc ctccacgact
      601 gcggcgggga agacgtaata gccggcttcg gaaagcggcg cggcggtcga agccagtaca
      661 ccccatgttc cgttgacatc gggcgattcg ttcagcggcg gaaccaccaa aattgaagcc
      721 ggtttgcttt ccttgaatga cgtgtagtcg aaatcgggcg ctttttgaac ttggcaggca
      781 gacagcgcca acacggcggc aagccctaaa atcaaaggtt tcatcgcttg cctcctttac
      841 cggttttcat caggaagtcc ataaatacgc ccgattcggg aaacagcctt ttctcttctt
      901 caaactggcg gaacgcgccc tctttgtctc ccgaacggga aagcagcagt cccagatggg
      961 cgtgcgcacc cggggcggca ttcatttttt tgttgccggc ttccacaaag tatttttcca
     1021 tcttttcggt ctgcttgccc aacgaagtgt cgtcgttttt caaaccttca tagacggtat
     1081 cgggatagcc gccgtaataa tacagggatt tttgcccgtt gccgccgcag gcggtcagag
     1141 ccaagaccgc cgcacacagc gacaaacggc tcaaggtttt cggattcatc atttctcctt
     1201 aacggttggg ttgccatgcg ccgttgtcaa cagcctgaac caggctgttg acggcttcgc
     1261 ggattgccaa gtctaaaact ttgccgttca aagtcgcatc gtagccggaa gtgccgccga
     1321 aaccgatgat ttcacggttg gaaagtgcgt attcgcccgc gccctgtgcg gaatagacga
     1381 tttcggaagt attgacgttg acgatattca gagccacttt tgcataggcg atttgcgatt
     1441 tgccgcgacc caaaatgccg aagagctgat gatcgccgac atctctgcgt ccgaattcgg
     1501 ttacatcgcc ggtaacgaca taatctgcgc ctttcaggtt atgcgctttg ccggaaatgc
     1561 cggattcctg ttttaatgcg ttcaaattgg tgcggttcag tacgttgaag cggttggtct
     1621 gttgcaggtg cgttactaga atggtttttg cctggctgcc caaacggtct tccccgtcgg
     1681 agaaaatgcc tttttggaag ctggagcggt tgtcgaatgt tccgacggaa atcggggtac
     1741 gaacaccgtg atattgcgta ttgtaggagg cgactttctc tacctcgaga ctgcgtgagg
     1801 attcggtcgc acagccggtc agtgaaacgg cagcggcggc aaggacaacg gcggtggaaa
     1861 cggttttcat aaaatttacc ctaaggtcaa gttaaggaaa taacgggttg tcattattgt
     1921 ccttatgtaa atttaagtca aggtgtttgt ctgtgcggga cggatgcgcg cggaaggtac
     1981 ggatattttt caaacggaat cgcggcgcgg gcgggatgcc tgccttttcc ggatttagag
     2041 gtaaacgatt tcgccactcc gccctttgct ttcggcactt gcccaccaga caaatgcggg
     2101 cagcacgtcc ccgtagcttt tgcgttcgct tttggcttcg cccggatggg atttgatgcg
     2161 ttgcggggaa ttgatggggc cggggacgag gacgttggcg cgcaggttgc cgaagcgttc
     2221 ccattcgtcg gcggcgactt tgcacaggta gttcaacgcg gctttggacg cgccgaagcc
     2281 gccccagtag gctttgggtg tttcgccgtg gctttcgccg acgaagatga cggacgcgtc
     2341 gggcgactgc ttcagcagcg ggaacagggc gcgggtcagc cccataggtg cgacggtgtt
     2401 gatgcggtat tggttgaccc attcggcgac ggtttggaaa tccagcggcg agagggcgta
     2461 aaaatagccg gcgcagtgga cgatgccgtc cagtttgcct tgcgtggctt cggcaatggt
     2521 ggcggcgaaa tgttcaaatt ctttttcttc cgcgctaata aggtcaaagc agatggcgaa
     2581 tggttcgggg tatccggctt cgacaatcgc gtcatacact ttttccagtt ttttctgatg
     2641 acgggcaacc aaaatcacgg ttgcgcctgc cgccgcatag gctttggcga cctgttcgcc
     2701 cagaccttgc gatgcgccgg ttactaagat ggttttgtcg gacagtgtcg ccatactttt
     2761 tcctttttgg ttgtcggtta aggtatttta gcgttttgcc gcaccttgta aagcgtcccg
     2821 atgtcggccg gcgttttcag acggcatcgc tcaggctttg caggcaggct tccgccgacg
     2881 cgaaacctga ttgtacggcg gcttcgagcg tggcggggta gtccgggtgg aggtagtcgc
     2941 cggcggggaa gatgcggtgc cggtgcaacc acgacaagtc cggcggcggg gcatcggctg
     3001 cggttgtggc gcgtttttcg gtgatgacgc gcacggcttc gggttcgccc aaatgcggaa
     3061 ggatgcgttt gaggtcggcg tgggctttgt ccgcccacgc ccggtttgca aacgcgccga
     3121 cgcggtcgga aacgctgatg acggcggaca cttcgttttc aggcagtccg agcctgcccc
     3181 ggcaaagcag ccattgcacc gtgccgtcgg caaggccggt cagcggggcg ggcaggcgga
     3241 cgggttcggc gtagcgcaga tagacggtgg tgatggcgtg gtagcgaagg ttttgatatg
     3301 ccgtctgaac gtgttcgggc gtgccttcgg gcaggagcgc ggcggcgtgg tagggcgcgg
     3361 tggcggggac ggcggcatcg aaagcttcgc cgttgacgag cactttcccg tccgggaggg
     3421 tgttcagacg gcatacgcgc gtttcgaggc ggatgtccgc gccgagccgt tgaagatccg
     3481 ccaaggcggg ttcggcgacg attgcgccca aatcctgctt gggtaggaga tagtcgctgc
     3541 cggatttttt cgtcagcacg ccgtcggaca aaacgttgca caacacgcgc aggcttgcgg
     3601 tttccaaagg cgtgttgagc gcgccccaaa ccaagggctg ccaaaactgc atcacggcgg
     3661 cacgcggcac gttccgctgt ttcagccatt gcgccactgt cgtgtcgggc tgtccgaggc
     3721 gtgcggactt ctgcaaatcg gacatatcgg caagcagttt ggctttgaat gcagtcggtg
     3781 cacgccgggc aagcagcacg ccgcccaaaa tatgcagcgg cgcgggcagg gggagggcgc
     3841 ggaactgcaa accgccgtgc atatgccagt gcagcggtac gcgcaaaaag gcggcacggg
     3901 gatccgaacc gatggttttc atcaggcgca acacgccccg gtatgcgccg agcaaaatgt
     3961 gctgcccgtt gtccaaaaaa ccgaaaccgt cggtatttcc ggccagtgtg cgcgccctgc
     4021 cgcccgcctg ccggccggct tcaaacaggg taacgtcggc gtgccgcgcc aaggtgacgg
     4081 cggcggacag tcctgcccag cctgcgccga tgacggcgat tttcgggcgc ggatgcggcg
     4141 tgttcatcat ttattcctcc aatggttttg cagccgtatc tatttccgtt tccgaaaatg
     4201 acggtagaaa aggatacagg ctaaaaataa aggcagcaga aagcgcgcat agggatacca
     4261 cggatggtct gcatgccagt atccgtaccg tccctgtgga acggtatcat agcggtaact
     4321 gtcggggaaa aagttgatcc aaaccgttac tgccagccat aaggctatat ccctgactgc
     4381 tttcatttac ttctgctcct gtttaaattc ccagcaattc catttcaaag cgcgaacgcc
     4441 aacgggattg cgcggttacg atgcagactt tcagacgatg gttgaaaccc cgtccggcgg
     4501 ggctgtggga atcgggtttg cctgacgggc ggcgggcggt atgcgcctta tgcccgttcc
     4561 ggcgtgccgg ggcgcggttt gaatccgaat aaccaggttt tcagggcaat gcgttttttg
     4621 cgcggcgaag ggagggcgat tttgtatttg aggacgtttt gtgcgccgtc tcggtcgatt
     4681 tcgttcaata gctcgtaata aaccgccgcc atgaccagtc cgactttttg ggctttttta
     4741 tcggcatcag gcagcagcga tacggcttcg cggtaggttt cgcgggcgcg tttgatttgg
     4801 aacgccatca attcggcaaa attgcccgtc gggctgcatt gcaaaatcac gcttgcgggt
     4861 acgtcaaacc gccgcatttc ctccatcggc aggtaaatcc gccccctgcg cgcgtcttcg
     4921 ccgacatcgc ggatgatgtt ggtcagttgc agcgcaagtc ccatcttgtc ggcgtattcc
     4981 agcgtttggt cgtctgaaaa ccccaaaatc cgcgcaatca ggcagccgac cacgcctgcg
     5041 acgcggtggc aatacagttt caattcttca aaactgccgt aacgggcttg aaccaaatcc
     5101 atctgcatcc cgtcgattaa ggcttccagt tcatatttcg gcagcttgaa ggtttcctta
     5161 acttgccgca aggcctgatt gacggggtgt tccggcatcg cgccgccgaa taccttgtcc
     5221 aaatcgccgc gccaccagtt caatgtcgcc tgtgcaacat cggggttgga acattcgtca
     5281 accacatcgt ccaattcgcg gcaaaaagca tataaaaccg ttaccgcatc ccgtttttcc
     5341 tgagtcagga aacggaagcc cgacaaaaaa ctggagcggc tttcttctgc tttttggcgg
     5401 caatagtcga gtcctttcac gatttatatt cctaatgatg ggcgggaaag gcggatttta
     5461 tcggcatttg gcggtagagg gcaatttcgg cggcacgacc taatccttag cggtttgctc
     5521 aactatcggc gcaaattctg ttaaaatgcc gctttccttc ctttacacac cgcaccgaca
     5581 ggcagaattt atggctcttt tgcagatttc agaaccgggt atgtccgccg ccccgcaccg
     5641 gcaccgtttg gcggcaggca tcgatttggg tacgaccaac agcttggtcg ccaccgtccg
     5701 cagcggcagt gccgcctgcc tgcccgatgc cgaagggcgc gttaccctgc cttccgtcgt
     5761 ccgctatctg gaaaacggcg gcattgaagt cggcaaaacc gccctgtccg cccaaaaaac
     5821 cgacccgctg aacaccgtca gctccgccaa acgccttatc gggcggacgc ttgccgatct
     5881 gcatcaaaat acgcactacc tgccttaccg tttcggcgac aatcaacgcg ttatcgaact
     5941 gcatacgcgg cagggggtga aaacgcctgt cgaagtgtcg gcggaaatcc tcaaaaccct
     6001 taaatcgcgc gccgaagaaa ccttgggcgg cgatttggtc ggcgtggtga ttaccgtccc
     6061 cgcctatttc gacgatgccc aacgccaggc caccaaagat gccgcgcgtc tggcgggttt
     6121 gaacgtattg cgcctgctca acgaacccac cgccgccgca atcgcctacg ggctggacaa
     6181 cgcctcggaa ggcacgtttg tcgtgtacga cttagggggc ggcacattcg acgtatccgt
     6241 attgcaactg accaaaggac tgtttgaagt caaagccacc ggcggcaaca gcgcgttggg
     6301 cggcgacgat ttcgaccacc gcctgttctg ccgcctgctc gaacaaaacg gactctccca
     6361 actcaacgaa caagacagcc aactcctgct ctcgctcgtc cgcgccgcca aagaacaatt
     6421 aaccacgcaa accgaagcgc gcattcaggc gacgctttca gacggcatgg caatcgacac
     6481 aagcatcagt cgcgccgagt tccacaacct gacgcagcat ttggtgatga aaacgctcga
     6541 accggtcaca caggcgttga aagatgccgg tgtcggtaaa aacgaagtca aaggcgtgat
     6601 tatggtcggc ggttcgaccc gtatgctgca cgtccaacag gcagtcgcca ccttcttcgg
     6661 acaaaccccg ctgaacaacc tcaaccccga cgaagtcgtc gcgctcggcg ccgccataca
     6721 ggcaaacgtc ctcgcaggca acaaaaccga cggcgaatgg ctgctgctgg acgttacgcc
     6781 cttgtcgctc ggtttggaaa cctacggcgg cttggcggaa aaaatcatcc cgcgcaattc
     6841 caccatcccc accgcgcgcg cgcaggactt taccaccttc aaagacggtc agaccgcgat
     6901 gacgatacac gtcgtacaag gcgaacgcga actggttgcc gactgccgca gccttgccaa
     6961 attcaccctg cgcggcattc cgcctatggc ggcgggtgcg gcgcgtatcc gcgtaacctt
     7021 ccaaatcgat gcggatgggc tgctgtccgt ttccgcccaa gaacaaagca ccggcgtaca
     7081 ggcgcaaatc gaagtcaaac cctcctacgg cttggacgac gacaccatca cccaaatgct
     7141 caaagacagc atgagcaatg ccgccgaaga tatggcggca cgcgcccgtg ccgaagccgt
     7201 agtcgaagcc gaaagcctga ccgatgccgt caacgccgcc ctcgagttgg acagcgattt
     7261 gctggatgcc gaagaattgc aacagattcg gcaaggcatc gccgatttgc aaggccgtct
     7321 gaaagacgga aaagccgaag acatccgtgc cgccgccgcc aaactcggca gcatcaccga
     7381 caatttcgcc gccaaacgca tgaaccgcaa catccaacgc gcgctgacag gccagagtgt
     7441 cgataatatt tgatacttaa acggtttcag acggcataga aataatccga tgccgtctga
     7501 aggctcgaaa acacttgaaa aacatcgata tggaaaagtc aggcattgtc tatctgatga
     7561 aaaccgtcat caagggcgtg tataaaatcg gcatttcgga tgtaagcaat tttgaaggca
     7621 gaatgcgcca tttggaaaac aacggttatg cgaacgttgc cggattggaa cgcatcctcg
     7681 ccgtcaaaac cgacaattac aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa
     7741 gcaggatagg cgataccgaa ttgttcgccg tggacgaaaa ccttgtgaaa cgtttgtttt
     7801 tatcgcttcg cggcgaaatc gtgttcccga aaaacgaaac ggcggaatcg gaatttgaaa
     7861 aaagcgtcca cgaacgcagg caggaaggga atgccgggtc aggccgcaaa caactgcttg
     7921 atttggtacg gcgcggacac cgggaatacc cttacgcgct gccccggctt ttggcgggcg
     7981 cggcatccta caagccgaaa aaatcgaaaa tccgcctttt taaagaagca tatttcggca
     8041 aaagcggcac gaggctgacc gacgaaattg cagacggcat ccatatttac acctgttttt
     8101 cgcgggcgga tttggaaaaa gcctattccg aatatttgga acttttcaaa tccgaatcgg
     8161 atgccgaagg cagaaagccg cagtaaggtg caaacagata ccgtacacgt tgaggagcag
     8221 atatgatggg cgattccgtc atttattatg tagaacaggc agacgaaccg gtaaaccgtg
     8281 ccgacgaacg cgcccgtaaa acattcaaat atttttggcg cgagcttttt tgggaacgcc
     8341 gccgcattat ttccgccttg gattttgcca tggtcaaagt cccttttttc caagacggcg
     8401 aagacggcga aatttgcgaa catatgtgga tcgacgatat ttattttgac ggtctttata
     8461 tttacggtgt gctgaacaat gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg
     8521 tttgcgttcc ggttgacgac atcagcgact ggatgttcgt gtgcaacggc atcccctacg
     8581 gcggctttac catacaggca atgcgcgggc agatgacgga agaggagcgc accgaacacg
     8641 atgccgcatg gggaatcgat ttcggcgatc ccgggcagat attgctggtg tatgaagaaa
     8701 aagaacatcc cgaaaatctg gaagagcatc cgatgtgccg gaactgtatt gacgattttc
     8761 ggcaacagtt gtcccaaaac tcggattatc tgcgggaaca ggacgaagac ggctatacgc
     8821 cgcttcatca tgaagccatc gcaggaaatg cacttatggt tcaagccatg cttgaatacg
     8881 gcgcaaatcc tgcctcaacg acatcggaag gctataccgc cctcgatttt gcctgcctga
     8941 cgggctggca aaatgttgcc gacctgctcg aaccgcgaca ttaggcagac agttttccga
     9001 aaacgaacac aaacactttt tacagaaaga caataaaaat gcccaaaatc accgtacttc
     9061 cacacacgac attatgcccc gagggtgcag tcatcgataa cgcacccgaa ggtaaaaccg
     9121 tccttgacgt gctgctcgac catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg
     9181 cctgcacaac ctgccacgtg attatccgca aaggtttcga cagcctagaa gagccgaccg
     9241 aattggaaga agacctgctc gatcaggctt ggggtttgga agccgattcg cgcctgagtt
     9301 gtcaggcggt tgtcgccggc gaggatttga ttgtggaaat ccccaaatac accatcaacc
     9361 acgcgcgcga agaacactga aaacaggccg tctgaagccg gcacgcttca gacggcattg
     9421 ttgcgcggat aaggcgcaat cgcccgaaaa caggcgttcg tacaggcgga actttcgatt
     9481 ctatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
     9541 ggcaaggcga ggtaacgctg taccggttta aatttaattc actatatatt gatttttatc
     9601 ggttttctga cggaataatc cagtgcggca tccgaggcgg attactcgga cgcgatgcac
     9661 cggtatttat cggttttgca gccggaaaaa ccgccggcgg gttatagtgg attaaattta
     9721 aaccagtaca gcgttgcctc gccttgccgt actatttgta ctgtctgcgg ctcgccgcct
     9781 tgtcctgatt tttgttaatc cactatactt ttagggcgac ggtcgggcag tatgccggat
     9841 agcgttccac tctcgcttct atattgattt tgattggttt tctgacggaa tgacccgatg
     9901 cggcatccgg gacggcttgt gttttttcct gcccgcctgc cggattttcc catccttgcg
     9961 tgaaaccgaa agagacggcg gcgcggcgga caagctcgag atagcgtcct tcaagctccg
    10021 gacaggcggc ggacacgctt tctaccgtaa ccgtgaaacc gccgcccgac ccggcaaggc
    10081 gttccgcaat ttgcgtgtag atgagccaac gttgggcgcg caacattgcc gaatcgcaac
    10141 cggcagccgc tttatccaag gcaatcaggt cgcgccggtt ttggtggtga aggcaaaccg
    10201 ttactctgga gaggatatgt tgcccgtcca atatttgata cagtttgcgt atcagcagaa
    10261 tcaggcggtc aaactcctcc atctccgaca gggaatcagg atggtcggaa gcggtataaa
    10321 ccagtttgga caattttgcg gcaaacatat tgttcatcaa tcttccttgt cggttgaaac
    10381 cccgctcttc ggggcggtag aatcagattt gtttgggagg gacaactcct cccagatcag
    10441 gacgacacat aggctggtgc ttgatgtgtt gtccggcgag ttgaaacatt cagcaatcct
    10501 caaggggcgg cagttttgcc gaaacatatt ctacacggct tcaatgccgg acgataaaag
    10561 gaaattcata tgaaatggac cgacacccag cgcatcgccg aagaactcta tgacctgcac
    10621 ggcgaaacca tcgatcccag aaccgtgcgc tttacccaac tgcgcgacct gattatggca
    10681 ttgcccgaat ttgacgacga ccccgcccgt tgcggcgaac gcatcctcga agccgtgcag
    10741 caggcatgga tagacgaggc ggaataagtt tcgggaatgc cgtctgaaat gcggcggtac
    10801 gcggttcgtg cttctgtttg cagcgggaat ggttttacca gtctcctttt ttcagcctgt
    10861 ccagttggcg gcggtcgcgc ttggtcggtc tgccgtcggg ataggcggaa gtgatgcggc
    10921 tgaattggtc gagctgtttg cgctcttccc tcaatgttgc cgttttcgcg tcctcttcat
    10981 acagaagccg cgcctcggat gccgggcggc gttggtggtt caaaccttta accttgattt
    11041 tatagggaag ggaattgagc gtcaggtcga taatatcgcc gatgtctatg gttttactgt
    11101 ttttgacctt cgagccgttt acttgaaccc tacccagttc gatgtgcttt tgcgcaaggg
    11161 aacgggtctt gaaaaaacgt gccgcccaaa gccatttgtc cagccgcatg gcggaagaat
    11221 cgtgcttgtc tttcatacga ttttgtttga aataattgaa tttgtttcga gtttagcata
    11281 agatacgccg ccttataact agtatatatg cactaatcca ctgttttcca tgctgtccga
    11341 acacaaaaag agggtatgga aaagccgttt tggacaataa attaactgcg gaatatgcac
    11401 aaatagcgta tgatagcggc agaatctgtt gatgagagct tcattctatg aaacctgttt
    11461 ttttggattt tgaacaaccc atagccgaac tgaccaacaa aatcgatgag ctgcgtttcg
    11521 tccaagacga gtctgccgtc gatatttcgg acgaaataca ccgtttgcag aaaaaaagca
    11581 acgacctgac caaatcgatt ttcagcaaac tcacacccgc ccaaatttca caggtttccc
    11641 ggcatccgca gcgtccctat actttggatt acattgaggc actgtttacc gattttgaag
    11701 aactgcacgg cgaccgccac tttgccgacg attatgcgat tgtcggcgga ttggcgcgtt
    11761 tcaacggaca aagcgtgatg gtcgtcgggc atcagaaagg gcgcgacacc aaagaaaaaa
    11821 tccgccgcaa cttcggtatg ccccgtcctg aaggctaccg caaagccctg cgcctgatga
    11881 agacggcaga aaaattcggc ttgcccgtaa tgacctttat cgatacgccg ggcgcgtatc
    11941 ccggcatcgg cgcggaagaa cgcgggcagt cggaagccat cggcaaaaac ctgtacgaac
    12001 tgacgcgcct gcgcgttcct gttttgtgta ccgtcatcgg cgaaggcggt tcaggcggtg
    12061 cgttggcggt cgccgtaggc gattacgtca atatgctgca atactcgacc tattctgtta
    12121 tctcccccga aggctgcgcg tctattttgt ggaaaaccgc cgaaaaggcg gcggatgcgg
    12181 ctcaggcttt gggcattact gctgaccgcc tgcaaaagct ggacttggtc gataccgtca
    12241 tcaaagaacc attgggcggc gcgcatcggg atttcgggca aaccatgaaa aacgtaaaag
    12301 ccgttttgga aaaacaactg cacgaagcgc aaagcatccc gcttgccgat ttgctttcgc
    12361 gccgtttcga ccgcattatg gcttacggca aattttcgga acaataattc aggtagaaca
    12421 agcagcaagc agtttgtctg aaactgcttg ctttttcttt atcgggacgg aaccgtgctg
    12481 actttagatg cgtttgagca atgcttgaag gattgttttc ctcaaggtct gaatggaaaa
    12541 aaaacagcgg tggcattaag cggcggcttg gattccgtcg ttttgctgca tctgcttgtc
    12601 cgcgccggaa aaaagggcgg ttttattccg gatgcattgc atatccatca cggcttgagt
    12661 ccccgtgccg acgattgggc agatttctgc caaaactatt gcgatatgct cggggtgggg
    12721 ctggaaacgg ttaaggtctg cgtggaaaaa aacggtttgg gcatcgaggc ggcggcaagg
    12781 caaaagcgtt atgccgcgtt tgccgaaaaa ggctttgacg ttttggcgtt ggcgcaccac
    12841 agggacgatc aaatcgaaac ctttatgctg gcggtcgcgc gcggcggcgg tttgcgcgct
    12901 ttggcggcta tgcccgccgt ccgccctttt ggggaaaaag gcatcatctg gcggcccttg
    12961 ctgccttttt cacgccaaga tatatgggat tatgcccaaa aacacggttt gccgaatatc
    13021 gaggatgaaa gcaataccga tacggcttat ttgcgaaacc gcttccggca ccgtattttg
    13081 cccgaacttt cggcgcagat tccccatttc gggcggcatg tgctgaacaa tgtccgcgct
    13141 ttgcaggaag atttggcttt gttggacgag gtcgtcgttc aggactgccg ttgggtttgc
    13201 ggggccggtt atttcgatac ggcgcggtgg ctgacgtttt ccccgcgccg gaaaacccat
    13261 attttgcggc attttctgaa ggaaaacggc attcccgtgc cgaatcagaa tgcccttgcc
    13321 gacattgccc gggttttgac ggaggcaaaa accggacgtt ggaacttgca aggctttgaa
    13381 ttgcatcatt atgcaggcag gctgtttgtg ttccgactgg aaaaaacgga taaactgcgg
    13441 tttttgaaag acaggcagat aagcggaaat ttaagggaaa tattgacggg gcagggattt
    13501 gtgttgaagc ggcatccgtt tgggcttcct gagcatcttt tggagcagga cggaattttg
    13561 aggacggtag cggcatcgga tacgttggcc atgggcggca tccataagga tgtgaaaaaa
    13621 atccttcagg ggaaacgggt tttgcctgtc ctgcgcccaa tttggccgct tgttgccgac
    13681 agcggaaacc gtccattggc gttggcaaac tgttgtgcgg atttccaata ctcggtttca
    13741 gacggcattt tgcccgtcca tcctgacttt cccattttat tttgataata tcgcaaacag
    13801 atttcggcgg cgttcagtcg ggtattgtcc ggttgcatat ttctaaaagg cttgtgaagt
    13861 gaaacacatc agttcgacca ataatgaaca catcagacac ctgcaccgcc tgttgtcgca
    13921 aggaaagttc agacggcaat acgcccaaac cgttttggag ggcgtgcacc tgcttcaggt
    13981 tttcctgcaa tccggcggga tgccggtcgg ggtatatatt cccgaagcga aaatgccgtc
    14041 tgaagaagtc cgtaaattga cggcggtttt gccggaagac gggttttttt ccgtttcaga
    14101 cggcatattg aagaaaatca gcagcctgac ttgtgcggat gatgtgcttg cgctgattga
    14161 tattccagat gcgggtgctt tgccggccgg cggcgattgc gtggttttgg acggcgtgca
    14221 agacccgggc aatgtcggca cggtgttgcg aagcgcggcg gcggcgggaa tcggcgcggt
    14281 cattttgggc aaaggttgtg cggacgcgtg gtcgcccaaa gtgctgcgag ccggaatggg
    14341 cgcgcatttc ttgtcggaga tttatccgca ggcggatttg gaaatatggt tggtgcgcta
    14401 taaaggccgt gtgtttgcca ccgccttgcg cgaggaaaag caggcggttt tgtacggcga
    14461 agatttgtgc gaaccgacag cctgggtgtt tggcaacgaa ggcgcggggg tcggtaaagc
    14521 agttttagat agggcggaca agtgtgtcag gataccgatg cacgatgcaa ccgagtcttt
    14581 aaatgtcgcg atggcggcga caatctgcct gtttgaacaa atgcgccaac gggcggcgta
    14641 ttgaggaaga gaaatgccgt ctgaaaaaat ctattacggc gtattgattt tcttatgtat
    14701 cgcttctatg ctgctgtcgc cgttttttta tgcgggtgct ttgaagccca agaaggcggc
    14761 attgcggaag gacgggcagt ggaaactcat ctgattgtcc aatgccgtgg cggcggcggt
    14821 tttggcttgg gtgtggtgga aatggttttg acagatattg ctcaaaatcg tgctaatgga
    14881 atccgaacaa ataaagaatt tggtaaaaaa tttgttaaat caacgaatta aagttttgtg
    14941 gaaaacaaaa cagctctaag caaatagggc gtttgtcggt aaatacggaa gagttgcggc
    15001 attatcgggc atctttaaca agtagtgccg tcttgacagg caatcggttt ttatgggcag
    15061 cttgcaaaat cgcggatata aaattgcgaa tcggttaaag tgtggggacg ctatgaaaaa
    15121 ttgcgaattt tttatgaccc gacaagggca atctatgata gcggtgcaga ttacttaact
    15181 agggaaaaac atagattagt cgtaattgca aatagtgctt gggggctatt gcttaattta
    15241 tcttgttatt atgacgaggt tttggaaaag cggaaaatac cgttcggcaa acaggaaatt
    15301 gatgacgata tggacaaagt gtccgccctt aagcggaagt ttaaagatat ttctgaaatc
    15361 aaagtagggg atggttggga atacccgttc aattatgagc agggaatgaa agaattagat
    15421 gaagtgctat tgaaatacat tccctttttt gaagaagaac gataaaggag gttgatatgc
    15481 gcgtatctaa aataattgga agtatgttgc ttgttacagc ggttcagacc gtattttcgg
    15541 caaatgttta cgcgtgccgc cataatggta aaaccagtta cagccaaact ccgggaaaac
    15601 attgtaccaa cgcgggtttg gggcgggacc gggtgtacag ttcggttaga ccggcagtaa
    15661 aagacagggc ggaagacgca ggggtcggcg attattcgga cacggtgagg gacgaacacg
    15721 tccaaaatcc gaaaggaaat gcacagaaag acggttcggc tgccggcatc aagccgcact
    15781 gattgaagcc gaatcagccc ttgcgctgtc ggacggcaaa atttgaacga ttggggaact
    15841 ttgatgaaac acatccatat tatcggtatc ggcggcacgt ttatgggcgg gcttgccgcc
    15901 attgccaaag aagcggggtt tgaagtcagc ggttgcgacg cgaagatgta tccgccgatg
    15961 agcacccagc tcgaagcctt gggtatagac gtgtatgaag gcttcgatgc cgctcagttg
    16021 gacgaattta aagccgacgt ttacgttatc ggcaatgtcg ccaagcgcgg gatggatgtg
    16081 gttgaagcga ttttgaacct cggcctgcct tatatttccg gcccgcaatg gctgtcggaa
    16141 aacgtgctgc accatcattg ggtactcggt gtggcgggga cgcacggcaa aacgaccacc
    16201 gcctccatgc tcgcatgggt cttggaatat gccggcctcg cgccgggctt ccttattggc
    16261 ggcgtaccgg aaaatttcgg cgtttccgcc cgcctgccgc aaacgccgcg ccaagacccg
    16321 aacagccaat cgccgttttt cgtcatcgaa gccgacgaat acgacaccgc ctttttcgac
    16381 aaacgttcta aattcgtgca ttaccgtccg cgtaccgccg tgttgaacaa tctggaattc
    16441 gaccatgccg acatctttgc cgacttgggc gcgatacaga cccagttcca ctacctcgtg
    16501 cgtaccgtgc cgtctgaagg cttaatcgtc tgcaacggac ggcagcaaag cctgcaagat
    16561 actttggaca aaggctgctg gacgccggtg gaaaaattcg gcacggaaca cggctggcag
    16621 gccggcgaag ccaatgccga cggctcgttc gacgtgttgc tcgacggcaa aaccgccgga
    16681 cgcgtcaaat gggatttgat gggcaggcac aaccgcatga acgcgctcgc cgtcattgcc
    16741 gccgcgcgtc atgtcggtgt cgatattcag accgcctgcg aagccttggg cgcgtttaaa
    16801 aacgtcaaac gccggatgga aatcaaaggc acggcaaacg gcatcaccgt ttacgacgac
    16861 ttcgcccacc acccgaccgc catcgaaacc acgattcaag gtttgcgcca acgcgtcggc
    16921 ggcgcgcgca tcctcgccgt cctcgaaccg cgttccaaca cgatgaagct gggcacgatg
    16981 aagtccgccc tgcctgtaag cctcaaagaa gccgaccaag tgttctgcta cgccggcggc
    17041 gtggactggg acgtcgccga agccctcgcg cctttgggcg gcaggctgaa cgtcggcaaa
    17101 gacttcgatg ccttcgttgc cgaaatcgtg aaaaacgccg aagtaggcga ccatattttg
    17161 gtgatgagca acggcggttt cggcggaata cacggaaagc tgctggaagc tttgagatag
    17221 cccgggcgat gccgtctgaa agcccttcag acggcatcgc ccggctgcgc ggcacaaagg
    17281 cggaaaaacc gtttgccccg tattttcaaa cgcgttacac ttgccgccgc tgttttcagc
    17341 catttgatta cccgcaaccg ccgtcattgc gccggcggtt tgcctgtcag cgtcattgcg
    17401 ccgctgtaaa tacgaaagaa cacattatga ccgtatcccc cgtcgccttg cgccgtaaga
    17461 ccgagtgcaa gcctcatccc accgcgcgct attggaaaaa atgcgatgtc gaagccctgt
    17521 tcggacttcc cttcctcgac ctcatttacc aagccgccga aatccaccgc caaaatttca
    17581 acccgcgcga aatccagctt tccacgctgt tgtccatcaa aaccggcggt tgtcccgaag
    17641 actgcgccta ttgtccgcaa tcggcgcacc acaataccaa tctgggcaaa gagcagatga
    17701 tggatgtgga tgaaatcgtc gaaaaagcca aaatcgccaa atcgcgcggc gcaagccggt
    17761 tttgtatggg cgcggcgtgg cgcggcccta aacccaaaga cgtggagacg gtttccgcaa
    17821 tcatcaaagc cgtcaagggc ttgggtatgg aaacctgcgg cacgttcggt atgctcgaag
    17881 aaggtatggc ggaagacttg aaagaggcgg gcttggatta ttacaaccac aacctcgaca
    17941 ccgaccccga ccgctacaac gacatcatcc acacccgcca acacgaagac cgaatggaca
    18001 ccttgggcaa agtccgcaac gccggtttga aagtctgctg cggcggcatc gtcgggatga
    18061 acgaaacccg cgccgaacgt gccgggctga ttgccagtct cgccaatctc gacccgcagc
    18121 ccgaaagcgt gccgattaac cggttggtca aagtggaagg cacgccgctt gccgatgccg
    18181 aagatttgga ctggacggaa tttgtccgca ccatcgccgt ggcgcggatt acgatgccgc
    18241 aaagttatgt ccggctgtcg gcagggcgca gcaatatgcc tgaagcaatg caggcgatgt
    18301 gctttatggc gggcgcgaac tcgatttttt acggcgacaa gctgctgacc acgggcaatc
    18361 ctgatgagga cggcgacaga atcctgatgg aaaagctcaa cctgtatccc ttgcagtttg
    18421 aaccggaagg cgaggtcgcc gaagtggaaa aagcctctgg gattaaagtg gattattgac
    18481 gattgaaaaa tgccgtctga aacccggaaa aaggctttca gacggcattt gtccggacgg
    18541 catttccaat atctttttac cggcgcgtga tgctgccgtc gggcgagaca tccagcccgt
    18601 tccccttggg gaaatcgctg cggttcagat aaataatccg cccgataacg gttttctcgg
    18661 ggtcgatttt ggggatttta tcgccgactt gagtgatggg gataatgttg ccggaaacga
    18721 accgcccctg tttgtcggtg ataattttaa aaatgggggc gatgccgctg atgccggagg
    18781 tgttgattgc gccgtaggtg gcaaagttgc cgccgctgta ggagatgaag cggtcgcggt
    18841 aaagttcgac ggcgcgagta acgtgcggcc cctgcccgaa tacgacatcc gcgccggaat
    18901 cgacggcaag ccgcgcaaac tcaacgacgt tgcccctgtt ttccccatag aagatttcgg
    18961 tatcgaacgg caggtgttcc gcctgtttcc cttccgcgcc gccgtggaac atcacaatga
    19021 cgatgtcggc tttctgtttg gttttggtaa tccgttttct aactttggca taatcgttca
    19081 gtttgacggc ggcaaggttg ggggcgaagg agacgaagcc ggatcttacg ccgtttttct
    19141 tcaggatggc ggtttcaaac ctgttttcga tgcccgaata tttgatgttc aattcgtcaa
    19201 ggttcgccct ttatgccgtt tccgcaccgc aaaccgccgg ggtcaagccc tcggcgcatc
    19261 ctgccggaac ggaatccccg tgctgccgat tgacggttca aaccccgcgc ccgtttcaaa
    19321 taccggcgat gtggacggac aggatgcgcc tgacgaaaag acagccgata ccgtttccat
    19381 tatcggcgtg ggcgacatta tgctcggcag caattatccg gtcgattacc tgcccgatac
    19441 caatattctg aaaaacgtcg aatctgcctt gcaagacgcg gacattaccg tcggcaacct
    19501 cgaaggcacg ctgtttgacg aaggcggtac gccgaaaaaa tgtgcaaacc cccaaaatat
    19561 gctatgcatt ccgaacgccc tccgcatacg ggcaatacct tgccgacgcg ggattcgact
    19621 acctcagctt cgccaacaac cacagcaacg acttcggcgc gcaaggcatc acggcaacgg
    19681 cggcgagcgg cagctctttt acatactcga tcgcgctaaa gccgctgccg ataacgaggc
    19741 caaaattgcg gaaaataccg ccatcgccca gataaatttg tccatcatca gacctttact
    19801 gttcagacga gacagcattt gccgcacgtt ttggggctta tctttcgatt tgcgctacgt
    19861 cgcgcaccgc gcctttgtcg gcggaagtcg ccatcgcgcc gtaagctctt aatgcggcgg
    19921 agacgtagcg gtcgcggttt ttaggcttcc atgctttgct gccgcgcgct tccatttcgg
    19981 cacggcgtgc ggcaagctct tcatcggaaa tggcaaggtg gatgctgcgg ttggggatgt
    20041 cgatttcgac ggtatcgcct tcgtgtacca aaccgatcgc gccaccttcc gccgcttcgg
    20101 gcgaggcgtg tccgatggac aaacctgatg tgccgccgga gaagcgtccg tcggttaaga
    20161 gagcgcaggc tttgccgagg cctttagatt tcaggtagga agtcggatac agcatttcct
    20221 gcatgcccgg gccgcctttc gggccttcgt agcggatgat gacgatgtcg ccagcgacga
    20281 tttggttgcc caaaatgcct tctactgcgt cttcttggct ttcaaacacg cgggcgcggc
    20341 cggtgaattt gaggatgctc tcgtccacgc ctgcggtttt taccacgcag ccgcgctcgg
    20401 cgatgttgcc gaacaagacc gccaaaccgc cgtcttgcga gtaggcgtgt gccacgtcgc
    20461 ggatacagcc tttttcgcgg tcgaggtcga gggttttcca catacggttt tgcgagaacg
    20521 cttgggtggt gcgtacgccg cccggcgcgg ctttgaagcg ttcgatggca cgggtgtttt
    20581 cgggattggt cacgtcccat tgttcaatcg cgtctttcag cgtcggcgcg tggatggtgt
    20641 gcacgtcggt gtgcagtttg cccgctttgt ccagttcttt caggatggcg aagataccgc
    20701 cggcgcgatg cacgtcttcc atatagtagt cgtggttgtt gggtgcggtt ttgcagatgc
    20761 agggcacgac gcggcttaag cggtcgatgt ctgccatttt gaaatcgaca ccggcttcgt
    20821 tggcaacggc caacaggtgc aaaatggtat tggtgctgcc gcccatcgca atatccatcg
    20881 tcatagcgtt ttcaaacgct tttttggtgg caatgctgcg cggtaacacg gtttcatcgt
    20941 tttgctcgta atagcgtttg gtgatttcga caatcatacg gccggcttcg aggaacaatt
    21001 ctttgcggcc ggcgtgggtc gccaaatacg aaccgttgcc gggcagggaa aggccgagtg
    21061 cttcggtcag gcagttcatc gagtttgccg taaacatacc cgaacacgag ccgcaggtcg
    21121 ggcaggcgtt ttgttcgact tcctcgactt gccggttgct gacattgtcg tccgccgatt
    21181 caatcatcgc gtcaatcaag tccaaacggc gttcgggctg gatgtttgcc acgccgataa
    21241 ccttgcccgc ttccatcggg ccgccggaga cgaagatggt ggggatgttc aggcgcatcg
    21301 cggcaatcag catgcccggg gtgattttgt cgcagttgga aatgcacacc agcgcgtcgg
    21361 cgcagtgggc gttgaccata tattcgatag agtcggcaat caaatcgcgg ctgggcaggg
    21421 agtacagcat gccgctgtgt cccatagcga tgccgtcgtc gatggcgatg gtgttgaatt
    21481 ctttggcgat tgcgccggct ttttcgattt cgcgggcaac cagctggccc atattgtgca
    21541 ggtggacatg gccgggcacg aattgggtga aggagttggc aacggcgatg atgggcttgc
    21601 cgaagtcggt ttccatcacg ccggtggcgc gccacaatgc acgtgcgccc gccatattgc
    21661 ggccgtgggt ggaggttttg gagcggtatt caggcatagt gtgtttcctt gtgcctatac
    21721 cgtctgaaag acagggctgt ttcagacggt atcgggtacg gttttttaga gtgggaaaag
    21781 agggtatttt ataccaagta tcggaatttt gcgggattga aacggcgtgc ggcaaaaaag
    21841 aaaatccccg caggaatgcg gggacgggtt caggcgcggg caatcgcgac ggctttggat
    21901 gcgtcccaaa aatcaacggg tgcatttaat acgggtttga cgatgcccgt ccgtatggcg
    21961 aaatcgccga aaccttcgcc gatattgcgt tctgccgccc atttgccgat caggtcgtcc
    22021 aattcggcaa ggatttcggg cagggtgatg ttttctttgt aaagacgggg gatgcgtacg
    22081 ccttcacggt cgccgccgat atggaggttg tagcgtccga cggctttgcc gaccagtccg
    22141 atttccgcca acatcgcccg tccgcagccg ttcgggcagc cggtaatgcg ggtaacgatg
    22201 tagtcgtccg acgtgccgtg tttcgccata atcttatcca gctcgccgat gaagtccggc
    22261 agcacgcgtt cggcttccgc cattgccagc gggcaggtcg gaaaggaaac gcaggacatc
    22321 gcattttcac gcagcttgct gacatcgttg cggattaatc cgtatgttcg ggcaaattct
    22381 tcgatttttg ctttgtctgc ttcggcgaca tttgccacga tgaggttttg gttggcggtg
    22441 atgcggaaat cgcctttgtg gattttggcg atttccaaca cgccggtcag aagctgtttc
    22501 ccgccttcgt caaccaaacg cccgctttcg atgaaaaggg ttaaatgcca gttgccgtct
    22561 atgcctttca cccagccgat gcggtcgccg cgcccggtaa atttgaacgg gcgtacgggt
    22621 tcgaacggca tacccatacg gcgttcaact tccgcgcgga agttgtccaa gcccatattt
    22681 tgaatggtgt agcgggtgcg ggcgtttttg cggtcgctgc ggttgccgaa gtcgcgctgc
    22741 gtggttacca ccgcttcggc ggccttcagc gcgtgttccg gaggcacgaa acccagttcc
    22801 agtgaaatgt tcggataggt tttggtgttg ccgtgttcca tcgaaagccc gccgcctgcc
    22861 aaaacattga agccggcaag ctgtccgtta ccgtctgaaa cggcgacgaa atccaaatcg
    22921 ttgccgtagc agtccacatc gttcaagggc gggatgacga ctgcggtttt gaattttcgc
    22981 ggcagatagg ttttgcccaa aatcggctcg tcttcttgaa ggaagtcgtc ggaactttga
    23041 acttttttgc cgtccaccca cacatccaga taaccgcgcg tgcgcggcag caggtgttcg
    23101 gaaatctttt tcgcgtattc gtaagcctgc cggtgcagtt cggactcgat cgggttggac
    23161 gtgcaaagca cgttgcggtt catatccgcc gccgtggcga tggaatccaa acccagtttg
    23221 tgcaagaggc ggtgcatcgt ctgcaacttg gctttcggca cgccgtgaaa ttggaaggtt
    23281 tgccggttgg tcagccggat ggagcggtaa tgactgtttt cccgggcaaa tttgtccagt
    23341 tctatccatt gggacggttt gatgatcccg cccggcagcc ggcagcgcaa aagcataaat
    23401 ttcaagggct cgagttttgc ctcggcgcgt tcggcgcgga tgtcgcggtc gtcctgctca
    23461 tacataccgt ggaagcggat gagttggaag ttgtcgcctt tgaagccgcc cgtgagcggg
    23521 tctttcaaat cgtccaaaat cgtgccgcgt aaaaaattgc tttcggtttt cagacgttcg
    23581 ttgtcggata gcggtttttc ttgccacgcc aaaccttttg tcttggtctg tacggtcatt
    23641 ttgtgttcct cccgattata tttaatcaat aaacatcacg ctgatagcgt ttttcttcgc
    23701 gcagcatatc caaatattct tctgcgccct cttcgtccaa atgtcctgcc ccgataatca
    23761 catccagcaa ggcggcttcc acgtcttttg ccatttttgc cgcatcgccg cacacataga
    23821 tatgcgcgcc ttcctgcagc cattgccaaa gtccttccgc ctgttcgcgg attttgtcct
    23881 gcacatagat tttttcttcc tgatcgcggg accaggcgaa atcgtacctg tgcaggaagc
    23941 cgtctttggc aaactgctgc cattcggttt gatagagaaa atcacgggca aaatgcggat
    24001 tgccgaaaat cagccagttt ttgccttccg cattttctgc ggcacgttgt tggacgaaag
    24061 cgcggaacgg tgcgacgccg gtgcccgagc cgatcatcac aatcggcttg cggctgtctt
    24121 cgggcagcct gaagccgtcg ttgcgttcca caaacacgcg caccgtgccg tcctcttcca
    24181 gccggtcggc aaggaaaccc gatgcgccgc ccgttctggc gcggccttcg tgttcaaaac
    24241 gaaccacgcc gacagttaaa tgcacttcat cgcccacttc cgcctgtgct gaagaaatcg
    24301 aatacaaacg gggtgcaagc ggacgcagta aacggatgaa ttgttctgcc gtcaggcttg
    24361 ccgggaagcg gtgcagcaca tcgacaatag gcgtgttttg cacgaaatcc tgcaaaacgg
    24421 cgttatcggc aatgatttta tcgagttctt cataatgggc gaacgcggca tagcctttga
    24481 cgaaagccgg agtgttttgc gtgagttcga aatgagatga aagtgcgcgc gcaaccggca
    24541 tcatctttcc gcccgcctgt atttccgttg ccggatcgat gccgagcagg tctaggattt
    24601 ccctgaccag tgccggatcg ttgtcaaacc aaacgccgag cgcatcgccc gggaggtagt
    24661 gcaaatccga accgctcaaa tcgatttcga tgtggcgcac gtctttatcg gattggcggg
    24721 cggtgatttt ctgattggcc agcagggcgg cgggaaaggg ggctgccttg cagtacctgc
    24781 catccggtgc cgtctgaagg ccggcggggg gcgttgtctg cggcgcgggc gttgcccggt
    24841 tttttgcggc ttcttctttt aagagtgcgg cgatattatc tgtccaggcg tttgcggagg
    24901 cggtaaagtc caaatccgca tcaacgcgtt cgagcagccg ttttgcgccc aattcttcaa
    24961 aacgccggtc gaaatcttta cctgcctgac agaaattcgg ataggaactg tcgcccaaac
    25021 ccagtacggc aaattggagt ttgtccaatt tcggggcttt tttgccgttc agcagtttgt
    25081 gcagcacgac ggcttcttcc ggcggttcgc cttcgccttg ggtggaggta accagcagca
    25141 ggcggcgttc gccggcgatg tttttcgcct tatagtcttt cagttcggcg cgactgactt
    25201 ggatgccggc ggcttccagg ctgtccgccg ctttgtcggc aacggatttc gcattgccgg
    25261 tttgcgaggc ggaaaggacg gttacggaaa aaggttctgc cgccggcaat gccgtctgaa
    25321 gcgcgggcag tcctgcagat gccccgtttc ctgcttttgc ccaagcgtag ccggacagcc
    25381 acgcccattg tgccgcgtcc agccccgaca ggagctgcgt gatttcgggc ggcagaggcg
    25441 gtaatggcgg atttgtgttc tgcatatcgt gttcactcat aaaatcatac ctgccgcaac
    25501 agtgccgtat gtcgcttcgt ctatcaggat aaacgaaccg gcggcggtgt tttccgcata
    25561 aggcgttgcc gtaacgggtt tttgaaggtt gatgcggact ttggcgatgt cgttcatctt
    25621 caaggattcc gcgccggcct cttgttccag cgtgcggaca tccaaaacgc tttcaatttc
    25681 cccgactttt gccggcacgg tttgcgtgcc gtgcttgagc aggtatttgc gcgcggtgtt
    25741 gagcggacgt tcgtcaaacc agcaaagcgt ggcttccaga tgtttttgcg gggcgagcgg
    25801 ggaattttta tcgacaaaaa ggtcgccgcg cgaaacatcg atgtcgcggt ccagccggag
    25861 ggttgccgcc tcgccggcaa aagcctgcgc cacttcccct ttcggcgtga tgatttcgga
    25921 cacttcggcg gtcagcccgt tcggttcgat gcggacggtt tgcccgacgg cgaccgaacc
    25981 gcgttcgatg cgcccctgat agcctcggaa atcatcggcc ttgtcggcat cttggcggac
    26041 gaccagttgc acggggaaat aaaaatcgtc ggcggtgcgg ctgacttcgt ccgcccccgg
    26101 cagggtttcc aaaatggaca ataagggttc gcctttatac caaggcatat tgccgccggg
    26161 gtaaacaatg ttgtcgccca agagtgcgga catcggtacg aaatgcgcgt ctttcaaacc
    26221 gagctgttcg gcaagtcggc ggtatgcctc cacaatggcg ttgaatttgt cttcgctgta
    26281 atccagcagg tccattttgt tgaccgccac cacaatatgc gggcagttga gttggcggag
    26341 gatggcggaa tggcgtttgg tctgcggcag aagctgcaag ggctgcgcgc cgaaatccag
    26401 ttgggatgcg tcaaccagca cgactgccgc cgaagcggtg cttgcgcccg taaccatatt
    26461 gcgcgtgtat tgttcgtgcc ccggcgtgtc ggcgatgatg aatttccgtt tcgccgtgga
    26521 aaaatagcgg tatgccacat cgatcgtaat gccctgttcg cgttcggctt ccagtccgtc
    26581 ggtcaggatg gagaagtcta tggcttcttt caaacctttg cttttgccgg attccaaggt
    26641 tttgatttgg tcggacagca gggctttgct gtcgtagagc agtcgtccga tcagggtgct
    26701 tttgccgtca tcgacgctgc cggcggtaat gaagcggagc ggggtttggt gttgtgccgt
    26761 catattttct tcctcatatc tgcttaaagg gtttttgaaa tttagaaata gccttctttt
    26821 ttgcgttttt ccattgccgc ctcgcttgcc tgatcgtcca gccgggtcgc gctgcgttcg
    26881 gaaatgtcgg caaccgctgt ttctctgata atctccgtcg gcgtggacgc ggtgctttct
    26941 accgggcagg tgcagctgat gtcgccgacg gtgcggaagc ggacatcaag gatttcggag
    27001 gtttcagacg gcattttcgg ggtgagcggc gttacaggga ccagcagccc cctgcgtctg
    27061 accacttcgc gcctgtggct gtaataaatc ggcggcagct cgaggttttc gcgggcgatg
    27121 tattgccaga tgtcgagttc cgtccagttg gaaatcggga agacgcgcat attttcgcct
    27181 ttgtgcagcc tggtgttgta cagcgaccac agctcggggc gttgcgcctt cggatcccat
    27241 tgtccgaact cgtcgcggaa cgagaaaatc cgttctttgg cgcgggcttt ttcttcgtcg
    27301 cgccgcgcgc cgcccataag cgcgtcgaag ccgtttgcct cgatggtttc caacaaggta
    27361 accgcctgtg ccgcattgcg cgaatcggtt tctttgcgta agaccaccgt gcctttggca
    27421 atggagtctt ccacgcgccc cactatcagg cgggcattga gttttgccgc ctgcgcgtcg
    27481 cggaaggcaa tcacttcggg gtagttgtgt cccgtgtcga tatgcaccag cgggaagggc
    27541 agtttcaccg gccggctgcc cagccggaag gctttgcagg cgagggcgag caggaccacg
    27601 gaatctttgc cgccggaaaa gagcagggcg gggttttcgc attctgccgc cacttcgcgg
    27661 atgatgtgga tggattcgga ttccaaccag tcgagttggg cgttgttcgg ttcggttttc
    27721 gtcataccat attccttatt tcttctgtct gatatttatg aattatttgt gcagcccgca
    27781 ttctttgctg tttctgcctt cccaccacca ccgcccggcg cggatgtctt cgcccgcctt
    27841 gacggggcgg gtgcaggggt cgcagcctat gctgggaaat ccttgccggt acaaatcgtt
    27901 gtaaggcaca ttgttggcga ggatgtatgc ccacacgtcg tgttccgacc agtcgaaaat
    27961 cgggttgtat ttgccgatgc cccgtccggc atcgtattcg gcaaacggca gttccgtgcg
    28021 tgtggcggat tgttcgcggc gttgcccggt aagccaggcg tccgcgcctg caatggcgcg
    28081 gttgagcggt tcggtttttc ggatgcggca gcattcgcgg cgcgcttcaa cgctgtcgta
    28141 aaaggcaaac ctgcctttgc tttccacata acggtcggca tcttctcgaa ccggccggaa
    28201 acgctttatc cgcaaatggg gatatgcgcg tccgagcctg tccagcaggt tcagggtttc
    28261 cgtgtggagc agccccgtat ccaaggtaaa aatgccgata ttgaggtttt cgccggcgat
    28321 aaggtcggta atcaccatat cttctgccgc aaggctgctg gcaaaccgtg catcccggtg
    28381 gctgccgaca atccggtgca ggcgttgttt gagggtttcc gttttttccg caagggcggt
    28441 ttcgccgccg gatccgatat gcggtatctg ccacagggcg ggtttgaaca gtgtcgtttc
    28501 catttttccc gccttatgcc gcccgttgtc cggcattcag tccgcccaat gcgggatacg
    28561 tctgcccgac ccggttttct ccttcgccgt tttcaccgaa ccaggcgagt ttttcgtgca
    28621 gccccaccac ttcgcctatg acaatcaatg ccggattcgg cgcggtttcg gcgagttcgg
    28681 caaggttggc gagcgtgccg gttgcggttt tttgagccgg cagcgtgcct tggctgataa
    28741 cggctgccgg cgtgtcgggc gagcgtccgt gctgttgcag ccgttcggca atcagggcgg
    28801 ctttgagcgc acccatataa atcaccaagg tctggcggct gcgggcgagg gtctgccatt
    28861 cgatgtcggg cgcatccgcc ttgcggtggc cggttacgaa aaccgcactt tgggcataat
    28921 cgcggtgcgt gagcgggatg ccggcatagg tggtcgcgcc gacggcggcg gtaatgccgg
    28981 ggacgaccga aaacggaatc tgatggcgtg ccaaggtttc caattcttcg ccgccgcgtc
    29041 cgaacacgaa agggtctccg cctttcaaac gcaccacgcg cctgccttcg cgggccagcc
    29101 tgaccataag cgcattggtg tcctcttgcg gggtgcgctc gccccgggcg cgcttgccga
    29161 caaaaatccg ttccgcatcg cggcggacga gggacagtat gccgtctgaa accagcgcgt
    29221 cgtaaagcac cacgtctgcc tgctggattt cctgcagccc tttgagcgtc agcagccccg
    29281 catcgccggg acccgcgccg accagcgaga cggagccgcc ttgatcattt tgacgacttt
    29341 gttccaattg gcctgccaat tcccgttcgg caagggtgtt ttgccggttt ttgacgaggg
    29401 cggcgaaacg tccgttaaac tgcttttccc aaaagcggcg gcgttcggta acggatttca
    29461 gtttgccctt gacggcatcg cgccaccttc ctgaaatttc cgccatatcg cccaaagacg
    29521 gcggcagcag ggcttccagc ctttcacgca gcagtcgggc gaggacgggc gcgctgccgg
    29581 agctggaaac ggcaatctga accgggttgc ggtcgataac cgacgggaag atgaagctgc
    29641 aatggtcgcg gtcgtccacc acgttgaccg gcttttggca gctttcggca agatggaaaa
    29701 cgcgccggtt gagggcttag tcgctgcttg ccgcaatgat gaggaaaacc gtgcggatgt
    29761 gttcggcacg aaattcttcg gcaagccaca ggattttgtt ttccgccgcc aacgcggaga
    29821 gttcggcatt caggtgtttt gcggcaaccc tgacctctgc gcccgccttc agcagcaggc
    29881 tgattttgcg tgcggcgacc gcgccgccgc ctacgaccaa tacggggcgg ccggcgaggt
    29941 tggcgaaaat agggaaataa ttcactggct gactcctttg ctgtttgccc gcaccttgtt
    30001 tccgatacgg tgcgtcgcgg catttttgtc ggaatgcggg tcattttaga caaaaggatt
    30061 ttccccggtt aaataataaa aaggtatttg ttagaagctg aaagctatat gggggcggct
    30121 gcggatgcgg cggttttccg ttttataacg gtttcggaag aaaaacggcc tgaagccgtt
    30181 tcgggcattc agaccgtttg cgtggtgagg ggatgccgtc cgaagggcga aaagggcttc
    30241 agacggcatt gatgtcgggt ttcaggacag gagcaggatg gcggctgcgg caagcgaggc
    30301 aaccgataat gcggcggcaa gcgcggcttt gcctgcaaag cggattgagg ttttgccttc
    30361 gatgtatttg aagccggtta tcatcgggag gatgaggttt ttctttttga atacgcggta
    30421 tgcggcgacg gcggcgatgt ggattgcaga aaaaacggcg agcagcttga aaaagttgag
    30481 gtggattttc cgcataaggc tgcccgtatg ttcggaaacc aaatggttga ggtagccgtt
    30541 ggtgctgaag gtgttttcat cggcggcaaa aagcccggtg ccgacttgga aggacacggc
    30601 ggccaaaagc gcaacgacca tcagtgcgcc caaggggttg tgtccgggct ggatgtgttc
    30661 gggaataccg tttttcagat agccgcgtat gcctgcccag ccttggacga aacgggaaaa
    30721 acgggcggta tcgctgcccc aaatgcccca gcagaggcga aatacgagca ggaaaaggac
    30781 gaacagcccg acgcgcgtgt gccattgcag catatcgccg ccggctttcg cgctatacca
    30841 cataaagggc agggacgcgg caagcagcca gtggaaaagg cgggtgggga ggtcccagac
    30901 tttggttttg tttttcataa tcggtttccg gcggtagaat cggtttgttt tcgagcctta
    30961 ttttaaccga ttggaggggc aatgtttccc gtttttcatc tttcaggcga gagccgccgc
    31021 cagatgcttc agacggcatt gcgttttccc catgttttca aagcccgtgc ggaagattcg
    31081 cacaaaggga ctttcggcac gctcgccgta gtcggcggat cggcagggat gagcggcgcg
    31141 cccgtattgg cggcatcggc ggcaatgtat ctcggctgcg gcaaagtgtg ggcgggtttc
    31201 aatcaggata cgctaccttt tgccgttatt gccggttttc ccgagattat gctggatacg
    31261 gcggacagtt tggccaaacg tcaagatata aacgcctggg ttgtcggttg tggattgggt
    31321 acaggtaggg cggcggtcgg aacgcttgcc ggaattttga cggaacacac ggacaagccc
    31381 gtcgttttgg atgcggatgc gctgaacata ttatcaaccg atgccgaaac ccgaaatctg
    31441 gcgcgcgggt gtaaaaacct gattttaacg ccacaccccg ccgaagccgc gcgcctgctt
    31501 ggaacgacgg ttgcgcaggt tcaggcggat cggacggcgg cagtgaggaa gataggggca
    31561 attttcggcg caaccgtggt tttaaagggg cacaaaacat tggttgcctc acccgatacg
    31621 gaaatctatg tcaacgaaag cggcaacgcg ggattggcaa cggcgggcag tggcgacgta
    31681 ttgggcggca tcatcggcag tctgctcgca cagggcgtgc cggtttttga agccgcctgc
    31741 gcgggcgcgt ggctgcacgg cgcggcggcg gatgtcataa aagaatcggc aggcattgcg
    31801 gcagggctgt tggcagggga aatcgctccg gcggcaaggt ggctgcgcaa ccggataact
    31861 aaaagtatgt aagaagatat agtggattaa caaaaaccag tac
//
LOCUS       DAWISC010000004        30440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWISC010000004 DAWISC010000000
VERSION     DAWISC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.004"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="WIW87_000123"
     CDS             complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="WIW87_000123"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ5173347.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="WIW87_000124"
     CDS             complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="WIW87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ5173348.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3535..3756
                     /locus_tag="WIW87_000125"
     CDS             3535..3756
                     /locus_tag="WIW87_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ5173349.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="WIW87_000126"
     CDS             complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="WIW87_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ5173350.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="WIW87_000127"
     CDS             complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="WIW87_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ5173351.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4773..5987)
                     /locus_tag="WIW87_000128"
     CDS             complement(4773..5987)
                     /locus_tag="WIW87_000128"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ5173352.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6016..6462)
                     /gene="iscR"
                     /locus_tag="WIW87_000129"
     CDS             complement(6016..6462)
                     /gene="iscR"
                     /locus_tag="WIW87_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ5173353.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6753..7925
                     /locus_tag="WIW87_000130"
     CDS             6753..7925
                     /locus_tag="WIW87_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ5173354.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8074..10851)
                     /locus_tag="WIW87_000131"
                     /pseudo
     CDS             complement(8074..10851)
                     /locus_tag="WIW87_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(10841..12214)
                     /locus_tag="WIW87_000132"
     CDS             complement(10841..12214)
                     /locus_tag="WIW87_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ5173355.1"
                     /translation="MKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFMKNRLEIAKELLM
                     KDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAKQIATITEFVLCY
                     SKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQYSDSLNFAIQCPD
                     GTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSGWSVCYKNYMLVD
                     NENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLLQYLIQIATSESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKALSRAFYQSVKNQ
                     AEKKDGE"
     gene            complement(12442..12894)
                     /locus_tag="WIW87_000133"
     CDS             complement(12442..12894)
                     /locus_tag="WIW87_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173356.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPANYTLN"
     gene            complement(13111..14034)
                     /gene="truB"
                     /locus_tag="WIW87_000134"
     CDS             complement(13111..14034)
                     /gene="truB"
                     /locus_tag="WIW87_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ5173357.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(14067..14438)
                     /gene="rbfA"
                     /locus_tag="WIW87_000135"
     CDS             complement(14067..14438)
                     /gene="rbfA"
                     /locus_tag="WIW87_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ5173358.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            14629..15873
                     /gene="clpX"
                     /locus_tag="WIW87_000136"
     CDS             14629..15873
                     /gene="clpX"
                     /locus_tag="WIW87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ5173359.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(15976..17172)
                     /locus_tag="WIW87_000137"
     CDS             complement(15976..17172)
                     /locus_tag="WIW87_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ5173360.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            17576..18076
                     /locus_tag="WIW87_000138"
     CDS             17576..18076
                     /locus_tag="WIW87_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173361.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18161..18715
                     /locus_tag="WIW87_000139"
     CDS             18161..18715
                     /locus_tag="WIW87_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173362.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            18729..18929
                     /locus_tag="WIW87_000140"
     CDS             18729..18929
                     /locus_tag="WIW87_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173363.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(19204..20592)
                     /locus_tag="WIW87_000141"
     CDS             complement(19204..20592)
                     /locus_tag="WIW87_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ5173364.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            20920..21867
                     /locus_tag="WIW87_000142"
     CDS             20920..21867
                     /locus_tag="WIW87_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5173365.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(21920..22252)
                     /gene="trxA"
                     /locus_tag="WIW87_000143"
     CDS             complement(21920..22252)
                     /gene="trxA"
                     /locus_tag="WIW87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ5173366.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            22550..23023
                     /locus_tag="WIW87_000144"
     CDS             22550..23023
                     /locus_tag="WIW87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5173367.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(23093..23914)
                     /gene="nadE"
                     /locus_tag="WIW87_000145"
     CDS             complement(23093..23914)
                     /gene="nadE"
                     /locus_tag="WIW87_000145"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ5173368.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            24111..25466
                     /locus_tag="WIW87_000146"
     CDS             24111..25466
                     /locus_tag="WIW87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ5173369.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            25759..27300
                     /locus_tag="WIW87_000147"
     CDS             25759..27300
                     /locus_tag="WIW87_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ5173370.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(27369..28418)
                     /locus_tag="WIW87_000148"
     CDS             complement(27369..28418)
                     /locus_tag="WIW87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ5173371.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(28427..28654)
                     /locus_tag="WIW87_000149"
     CDS             complement(28427..28654)
                     /locus_tag="WIW87_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173372.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            28643..29275
                     /gene="pdxH"
                     /locus_tag="WIW87_000150"
     CDS             28643..29275
                     /gene="pdxH"
                     /locus_tag="WIW87_000150"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ5173373.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(29405..30415)
                     /locus_tag="WIW87_000151"
     CDS             complement(29405..30415)
                     /locus_tag="WIW87_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ5173374.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
BASE COUNT         6835 a         7414 c         8095 g         8096 t
ORIGIN      
        1 tgtttttaat tgactataat ccgctatatt gtgagaagct ggatgacgga tgagggataa
       61 aataaatgcc gtctgaatct tcagacggca tcgggagatg attttcagtt ctcgacttcc
      121 ggttcggtta cattggaaat tacagaagcg tcggagagtt cggattcgtc ttcggcaaca
      181 cgttccagcg ataccaaggt ttcgccttcg tccaagttaa tcagtttcac gcctgctgcg
      241 gcgcggccgg tttcgcggat ttgttcgact ttggtgcgga taagtacgcc gccgctggta
      301 atcagcatca aatcgtcggt ttcgccgacc aaggttgcgg cgaccaaatc gccgtttcgc
      361 tcgccagtgt taatggcaat attgccttgc ccgcctttgt ttttgcggct gtaatcggca
      421 atcggggtgc gttttccgta tccgttggcg gtggcggtta aaacttgcaa accgctttct
      481 tcggtttcag gggcgaaggt aatcaggctg acgattttgc cgtcggcagg caggcgcata
      541 ccgcgcaaac cgccgctgcc gcgaccggac gggcgaacac cgtgtttgcc gctcggcagt
      601 gcgttttcgc tgtcggcggt ttcatcttcg atgccgtctg aaatttcggt ttcgatgtcg
      661 gcatcttccg cttcgtcgtt gccggatttt tcccagtatt cgttgaagcg gatggcttta
      721 cctaagttgg agaacagcat gatgtcgtcc gcaccgcctg tttgcgcagc gccgacgagg
      781 tagtcgcctt ctttgagcgc gatggcttta atgccttggg cgcggacgtt tttaaaggcg
      841 gaaagttgga cttttttcac cattccctgc gcggtggcga agaagacgta ttggtcttcg
      901 gggaactcgc gtactgccag aatcgcgctg actttttcgc cttcttccaa ctggatgacg
      961 ttgttaatcg gacggccgcg gctgttgcgt ccgccttcgg gcagtttgta aaccttaatc
     1021 caatgacact tgcccaaatt ggtaaagcac atcaaataat catgcgtgtt ggcaacaaac
     1081 agggtttcga taaagtcttc gtctttggtg gcagccgcct gtttgccgcg cccgccgcga
     1141 cgctgcgcct gatagtcggt ggtcggctgg gttttgatat agccgccatg tgtcagggta
     1201 acgaccattt cgcgttgcgg aatcaggtct tcatcggcaa tgtcgccgcc gaacgggttg
     1261 atttcgctgc ggcgttcgtc gccatagttg gttttgattt cttccagttc gtcgcggatg
     1321 atttgggtaa tgcgttcggg tttggagagg atatccacaa agtcgatgat tttacccatc
     1381 aggtttttgt agctttcgac aatttcttct tgatcgaggc cggtcaggtt tcgcaggctc
     1441 atgcgtaaaa tagcatctgc ctgaatctcg ctcaggtaat aaccttgctc tttcaagccg
     1501 atgtttgcag ccaatccttc cggacgcatc atttccaaat ccagaccgga acgcgtcagc
     1561 atttcttcaa cgaggctgct gcgccaaggg cgcgcaagca gtttgtcttt ggcctcggct
     1621 gcgttgggcg attctttgat gagcttgatg atttcatcga tattggacag tgcgacggct
     1681 ttgccttcgg caatatgccc ttcatggcgt gccttcttca gccggaaaag cgtacgtcgg
     1741 gtaacgactt cgcggcggtg gcgcaggaat tcggagagaa tctgtttcag gttcaacagg
     1801 cgcggttgtc cgtcgaccaa aaccaccata ttgatgccga aactgtcttg cagcggagtc
     1861 agtttgtaga gttggtttaa gacgacttcg gcattttcgt tgcgtttcag ctcgataacg
     1921 acgcgcatac cggatttgtc ggattcgtcg cggagctcgg aaatgccttc cagtgttttt
     1981 tcccgaacca aatcgccgat tttctcgacc agcttggctt tgttgacctg ataggggatt
     2041 tcgtcgataa cgatggcttc gcgttcgccg tttctgccta tgggttcgat atgggtctta
     2101 ccgcgcataa cgacgcggcc gcggcctgtt ttatagcctt cgcgcacgcc gctcaagccg
     2161 tagatggttg ccccggtcgg gaagtcgggg gcttggataa tgtcgatcag ttcgtcgatt
     2221 tcggtgtcgg gtgcatcgag caggcgcagg caggcattga cggtatcaga aaggttgtgc
     2281 ggcgggatat tggtcgccat gccgacggcg atgccggacg agccgttgac gagcagtgtg
     2341 gggaaacggg tcggcagtac aagcggctcg tgttcgctac cgtcgtagtt cgggccgaaa
     2401 ttgacggttt cttcctcaat gtctgccagc atttcgtggg aaattttcgc catgcggatt
     2461 tcggtgtagc gcatggctgc ggcggcaagc ccgtccaccg atccgaagtt gccctgtccg
     2521 tctatcagca cataacgcat agcgaaattt tgcgccatac ggacgatggt gtcgtatacg
     2581 gcggtatcgc cgtgggggtg gtatttaccg atgacgtcgc cgacaatgcg cgccgatttt
     2641 ttgtaggcgg cattccagtt gtttttcagc tcgtgcatcg cgtacagtac gcggcggtgt
     2701 accggcttga gaccgtcgcg aacgtccggc agcgcgcgcc cgacaatgac gctcatggcg
     2761 tagtcgagat agcttttgcg catttcgtct tcaaggctta ccggcagggt ttcgagggcg
     2821 aatttgtggt cgtggcggat ggttgcgtcg gtcatggttt caatgtttcg tatggcaaaa
     2881 aattgttgct tattttagca tattttgacg cggaacggtg cggcggttac gccgtctgaa
     2941 acacggtgcg gattataatg ccgaggaaat ttcgttgcgg agtttgtcga gaaacctgcc
     3001 ttggcggact tgttgtgcgg ctgtgtcgta atcttgtcgg gcaaacgact gtttcagacc
     3061 gcagaacagt ttttcttgtt cgtcgcggat ttcgttgtcg agatttttca aggattcaag
     3121 gtcgttgcct gcccgtgcct ccatcagcgt ttcgcgccat tccatttgct gcataaggaa
     3181 ttcgggggcg aaagcggtat gctccggcgc gtcggcatcg atgcccgatg ttttcagcag
     3241 gtaggcggcg cggtcgatgg ggtttttcaa ggtgcggtag gcatcgttga tggtggaaga
     3301 catcatcact gcctgctttt gctcaaaggc ggaagctgaa gcgaatttat cgggatggaa
     3361 acgggcggcc aaggcgcggt aggtttgttc caagttttcg gtgtcgatat cgaaagcggg
     3421 ttcaatccgg aagagggtga aatattggga catagtagga tgataaatgt aagattttgg
     3481 cagaaaactg tttttgcctt ataatctgcc gcttcttaaa cgaaaggact gaatatgggc
     3541 ggcaaagtgc agcacaataa aggcaaaata cgcgacaatg ctttaaaagc cttagtgaaa
     3601 tccgatttgt tccggcacaa ggtggaacgg aaaaggaaag gcaaaggcag ctacaacagg
     3661 caggaagcga aaaaatggcg ggacggtttt gatacggtcc cgccgttttt atgccttaaa
     3721 cgtggaagct ttcgccgcag ccgcaggagt ctttgacatt ggggttttca aatttgaaac
     3781 cttcctgcaa accttctttg gtgtaatcga cttgcgtgcc atccagataa accaggcttt
     3841 tcggatcgat ataaatgcgc gcgccgtgtc cttcgaaaat caggtcgtcg ccatcggctt
     3901 cgtcgacaaa ttcaaggttg tacgccatcc ccgagcagcc gctggttttc acacccaagc
     3961 gtacgcccaa gcctttgccg cgtttggcga gatagtcatt gatgtgtttt gcggcattct
     4021 cggtaagggt aatcatattt cttccttgtt gtaccgcccc ggacggacct gaagcggcgg
     4081 gtggttcgga cggcattgcg ggatgatgcc gtctgaaggg ctttatctgt tttcctgacg
     4141 tttgcggtag tcggcaacgg ccgcttttac cgcatcttca gccaagatgg agcagtggat
     4201 ttttaccggc ggcaattcca actcctcggc gatttcgctg tttttgattg ccagcgcgtc
     4261 atccaggctt ttgcctttaa cccactcggt aatcaggctg gacgaagcga tggccgagcc
     4321 gcagccgtaa gttttaaatt tcgcatcttc gatgatgccc tcgtcgttca ctttgatttg
     4381 caggcgcatg acgtcgccgc aggcgggcgc gccgaccatg ccggtgccga cggaatcgtc
     4441 tcccttgtcg aatgtgccga cgttgcgcgg attttcatag tggtcgatta ctttatcgct
     4501 gtatgccatg atgtggtttc cttaatgttt tttgatggtt taagtggttt gtttgccgat
     4561 tttcagacgg cctgaagttt agattttgca cgccccgcct tcgcagccgt cgtcgttttc
     4621 cgaatcgacg ccgccttcca cggtaacgcc gtcaaaatct tcaagcaggt tgtcgagatt
     4681 gtccaaatct aaatcttgtt cgttcatatt ttctcttgat tgctttgttt ttggaatgcc
     4741 gtctaaaggt tcggacggca tgttggtacg ctttaatgcg ctgcccattc aatcgagttc
     4801 aaatcaatcc cgtctttgaa catttcccac agcggcgaca gttcgcgcag tttgccgatt
     4861 ttggatttaa tcagttcggc ggcgaattgc acttcttctt cggtggtcat gcgaccgaag
     4921 gtgatgcgca gggatgagtg cgccagttca tcgttgcggc cgagcgcgcg caggacgtag
     4981 ctgggttcga gcgaggcgga ggtgcaggcg gagccgctgg atacggcgag ttctttcact
     5041 gccataatca ggctttcgcc ttcgacgaag ttgaagctga cgtttaggtt gttcgggacg
     5101 cgatgttcga ggtcgccgtt gatatagact tcttcgatgc cttcgatacc tttgaggaaa
     5161 atatcgcgca gtttcaggta gtgtgcagtg tcttgtgcca attcttcttt ggcaatgcgg
     5221 aaggcttcac ccatgccgac gatttgatgg gtcggcaatg tgccggaacg gaaaccgcgt
     5281 tcgtgaccgc cgccgtgcat ttgggcttcg aggcggacgc gtggtttacg gcgtacatac
     5341 agggcgccga tgcctttagg gccgtatact ttgtggccgg acatagacag caaatcaact
     5401 tttgcggctt caacatcaac aggcactttg ccgcatgctt gtgctgcgtc aacgtggaaa
     5461 atgattttgc gttcgcggca gatttcgccg atggcaggaa tatcttgaac cacgccgatt
     5521 tcgttgttta cccacattac ggaaacgagg atggtgtctt cgcggatggc ggctttcagt
     5581 acgtctaaat caaccaaacc gttttcttgt acgtccagat aagttacttc gtaaccttgg
     5641 cgttcgagtt cgcgcatggt gtcgagtacg gctttgtgtt cggtttttac agtgatgagg
     5701 tgattacctt tagatttgta gaagtgcgcc gcgcctttga tagcgaggtt gttggactcg
     5761 gttgcgccgc tggtgaaaac gatttcttta gagtcggcgt taatcagggc ggcaatgtct
     5821 gcacgtgctt tttctacagc ttcttctgct tcccagccga agctgtggct gttggaggct
     5881 gggttgccga aggtttcggt cagataggga atcatttttt cggcaacgcg tttgtcaacg
     5941 ggggtggtgg cggcgtagtc gaggtaaacg ggggttttga cggtcatggt ttgctctttc
     6001 tttttcgggt gttatttaat ggatgtgtgt aaattggacg acgcggctgc cgtcgccgtt
     6061 gtttttctgt tcgatgatgc tttgcagggt aacgctgccg aggtagtcgt tgatggtttt
     6121 gtttaaattc tcccaaagat cgtgcgtcag gcagggcgcg ccgtggtggc agttggcttt
     6181 gctgccgcat tgggttgcgt ccagccggtc ttcggcggcg gcgatgattt gggcgatgtt
     6241 gatgcgtgcc gccggtgcgg cgaggatgta gccgccgccg ggcccgcgca ggctttcaac
     6301 aagtccggcg cggcggagtt tgccgaacaa ttgctcgaga taggagaggg atatgttttg
     6361 gcgttcgctg atggcactga gtttgacggc gccggtttgc gcgttcatcg ccaaatccag
     6421 catagcggta acggcgaaac gccctttggt ggtcagtctc atggtggtgg ttgcccatgt
     6481 cggttttttt attagtgggt ggaattgtcc aatatctgag tgtttcagtc aagtatgagg
     6541 gcggattgtg tgattggctg tttaaatcga tagattgatt gatttttaat attttgattt
     6601 taatttgtta tttttgaatt ttaaacctta tgtcgcttaa agtagggagc tgcggcggat
     6661 gcggcaaatg ttgaaatgat ttgccttata atcaatattg atatacatta tcgatttcat
     6721 ataaagttat tcaggtattc agggggcgac aaatgaaacg tgatttgagc aaaatgacct
     6781 gcatcgaaga cttgcgccgc gttgccaagc gcaaaatgcc gcgtatgttt tacgattaca
     6841 tcgattcggg ttcgtggaca gaaactacct accgtgaaaa cacttcggat ttcaaagaca
     6901 tccgcttccg tcaaaaagta ttggtcaata tggaaggccg aagcctggaa accaaaatga
     6961 tcgggcagga tgtgaaaatg ccggtggcga ttgcgccgac gggtttcacg ggtatggcac
     7021 acgccgacgg tgaaattttg gcggcgcggg cggcggagaa gttcggcatt ccgtttacgc
     7081 tgtccacgat gtctatctgc tcgattgaag acgttgccga gaacacttcc gcaccatttt
     7141 ggtttcagct ttatgtgatg cgcgaccgcg agtttatgga aaacctgatt aagcgcgcga
     7201 aggatgccaa atgttcggca ttggtattga ccgctgattt gcaggttttg gggcaacgcc
     7261 acaaagacat caaaaacggc ctgtccgcgc cgccgaaacc gaccatcgcc aatttaatca
     7321 atctggcgac caagcccgaa tggtgtatga aaatgctgaa cacggaacgc cgcacgttcc
     7381 gcaatatcgt cggacacgcg aagaacgtcg gcgatttgtc ttcgctgtct tcatggactt
     7441 ccgaacaatt cgacccgcgc ctgagctggg acgatgtggc gcgcattaaa gatttgtggg
     7501 gcggcaagct gattatcaaa ggcattatgg aacctgaaga cgcggaaaaa gcagcgaaaa
     7561 gcggcgcgga cgcattgatt gtttccaacc acggcggtcg acagctcgac gataccgtgt
     7621 ctgccatcaa agccttgccc gacatcgtca gcgcagtggg cagcgacatc gaagtttgga
     7681 tggacagcgg catccgcagc ggtcaggata ttttgaaggc gtgggctttg ggcgcgaaag
     7741 gtacaatgat aggtcgggcg ttcctgtatg gcttgggcgc gtatggagaa gaaggcgtaa
     7801 cccgtgcgct ggaaatcctg tataaagaaa tggatatatc tatggcgttt acgggacacc
     7861 gcgatattca agatgtcgat gcttcgattt tgagaggcaa agattggggc agggaaacgg
     7921 tttagatact gaaaaatacg atgccgtctg aacacagggt tcggacggca ttaaaatttg
     7981 gctgtatttt aataatgtgt tgattaaata tctgacaaaa cgcccttttg taaaggaagt
     8041 tttgtcaaac ggggttgcct gcaatgcacc actctatttt tcaagcgatg ccgcccctaa
     8101 agggaatgac tttgtaaatt gttcattttc ctgattgtca gtaaaaaacg gcaaaccgat
     8161 caaacgataa tgcggtgtat ctttttgcag aatcttatcc gtcccgtatt ccgctgtaat
     8221 tgcctttaaa aaagcgtctt tccacgaatc attttccacc aaatgctcac cttttggttc
     8281 aatgaaaatt tggtaatgca aaaagtcatc cacaccattg gaagaagatt tttgtttatt
     8341 tttcagcaat aaaataaagt ccggcataaa tccttcacca tcggaaaagt tattcaattt
     8401 aaacacttct tcattacgga ttaaatgaac atcatattta gacttcaaat cgcccaaatg
     8461 ctctgaaata aattgaatta acgcttcttc caaactcgtt ccagcaaaat tatccatcac
     8521 ataccaatca ttttgcgtgg caatggcagt ttttatatca tcttttttga cccatttttg
     8581 ttttggtgtg ccaaaaattt cccacaattt tttaggcgta aattcttttg taccgataaa
     8641 tggcatatca ctttcattca aatgtttttc aaccatttcc aagattttta ggcagcctgc
     8701 aagtttgtca tctgggctga tctgtttgtc ttgccctaat cccaaaaatt caatttgtca
     8761 atcttttaac aagttatttt gcaattcatt gcgattgtaa atgttgagtt tgctttgcaa
     8821 gcggtcaaaa tggaataaag attgaccatt ttttcctttg atatgcaggg atttattgaa
     8881 aatgtgccgt tccatttcac tcatttttat gatttgagta aaattatttt gtgtgtcgat
     8941 ttgtcgggct atttcatcat tttcatcggc tgtaaattgc gtttcctgca acagttgatt
     9001 gccgtgaact tggaatggaa gcgtttgcgg attggctttc aggctgtctg cattattggc
     9061 tctggcattg ggattgggga tttttttatt tgcccaaatt aacaactctc taaaatcctg
     9121 attatcggca aattcagatt tgagtttaaa tgttgccaat accttatcat cgtctttttc
     9181 aggcaaataa ccgtcttttc gtaactcgtt tttcagttct gtaatatagc gagattgctc
     9241 atcgtgcgtg taataaaaca attcttccaa aatacgcaat tcgtgttgca tatcgttgtc
     9301 aaatttgcgt ttattcggct gtttaccttc aaacgcaaat ggaaaataac gcacgccgcg
     9361 accaatcaac tgcttttccg atacagtagc ggcagccgtt ttgcctgatt ttttatttga
     9421 accgccgccg ttttgccctt catacaaacg cacaatatca aacaaattca gaacgtccca
     9481 accttcggtt aatctgtcca ccgtaaaaat ggcacgaatc ggattatcag ccgcttccaa
     9541 attattcagc aatttttctg tttcgctgtc ggttttttcg gttttggttt tgttggtttc
     9601 ggagttggta ataatcacat tgtgtttttg ataattttgt tttacccaat ctgccaaatg
     9661 tgcaaactca acgccttttt cctgcataaa ttttagggct tgttcagtgc gggttttgcc
     9721 ttgttcgttg gcgttatcgc tatcgctatc gttcaagctt gttgaaaatg tagttaaaaa
     9781 cgaaaaatca accgcctgca cattttctgc ccaatttaaa aatgccagat aatccgcttt
     9841 tgattcatca atcgtcttac ttctaaacaa catcacaggc ttgaaattgg caatgccata
     9901 tttcaacgca attcgatgtc gataccaagc aaacaataaa gcgtgtaaca ctcgctcttt
     9961 cttacccagc gtactggata ccaaattgat ttctttggta taaccttttt gcaaaaattc
    10021 ttttaagcca aattttgtga tgattttatc agcgtatttt tgttgtacat cggcattttc
    10081 aggcagcgtg gcggtaaatt ccaacagcac attttggcta tgattgccat ttttattgag
    10141 taacaattcc aaaaccatat gctcccagcc tttacgttca atttcggcat tgctggtgcg
    10201 gtcgttcatt tccttttcta aatctaattc gccttgtttt ttgcctttgg tttgcgcgtt
    10261 taaatggtgc gcttcatcac ccagcatcac aaggttcaat ttgtgcaaat ccgccaatgt
    10321 ggtttgattt tcccgccggg tgcgaatatc gttatacagc ttttgaatgc tggtaaattt
    10381 aatttcaatg ccgtctgaat gtgggctaaa tgtctccact ttgcgaatag gaattaccgt
    10441 atcgccctgc aaaatcttct cggtaaataa aaattttgcg tgcgtcggat cggtaaaatt
    10501 attttccgtt ttatccacga tattgttttg attcacaaaa aacagaaaat gccgataacc
    10561 tttttcaaaa taatacaaaa tcaacgccgc catcatcatc gttttgccag cacctgttgc
    10621 catattgaac agcaaatgcg ttggcttatt ttttaaatca gggaaatcgt ctagctttga
    10681 agtgcggtca aaaatcaaaa agttttcaag ggcagatttt tgccattcaa aaaaatcata
    10741 ttttaagttt ttggacgtaa attctggcat ttcaagtaat tcaggcttgt attttttaac
    10801 cgctttacgt tcattttcaa tcacttcaaa caacgtttta ttattcgcca tcttttttct
    10861 ccgcttgatt ttttactgat tgatagaatg cacggcttaa ggctttatca tcatcgttca
    10921 ggcaatctgc aaattgttcg tcatccattt cggataatga aacatacatt tgatttaaat
    10981 ccaacatttc aagcaccatt tgtttttgtt cgtctaatgg caaagattga aattcaggct
    11041 cttgaatgat ttgactaaat tcatttacgc tgacgttgta tttcaagaaa tagcgttcgc
    11101 ataaaccttc aaacagcgtt ttgatgccgt ctgaatcttc gcaagccaaa atttgttgtt
    11161 ttgcggtttc gttaaatggg gcaagttcgg cataaacaaa ctcgccacca ccttgccaat
    11221 tcacggcttt ggaaataccg ccttgctcgc catcaatcac ttttttcaag cgttcaacag
    11281 caagcgtttc aatataatcc atttgttcaa taccgatata ttggcggttc attttgtggg
    11341 caacggcggc ggttgtgcca ctaccaagat ggtagtctaa gacgatgtcg gattcggatg
    11401 tggcaatttg aataagatac tgcaataagc tctcaggttt tggagtagta aaaactttgc
    11461 tgccgaacag ttttttcaat tcatctgtcg catgtgtatt taagatatcc tgtattaagt
    11521 tcttataggg agcagaacgt tctatcggct tgttttcgtt atcaaccaac atatagtttt
    11581 tataacatac actccatcca gattttttag actttgattt tctaaactcc aaaaaaccgt
    11641 ttgttattgc ccaatcaatt ttatttttac tccatttcca tatccagcca tcattgacaa
    11701 attcagtcct gccattcgga tacgtaaaag tgccatctgg acattggatt gcaaaattca
    11761 aactgtcact atactgcaat ccccctctat ctaaattgtc gatataatat ttgcctcttt
    11821 cctgttcaaa cttatcactt aatttgtatc tctctgtatc ataagaaaac gtgtttttat
    11881 ttaatttaac tgttttaaag ttctttgagt aacataagac aaactctgta atagtcgcta
    11941 tctgtttggc atcggacgca cctgtctttt tttcccaaat aaaattgcag atgaaatttt
    12001 catttccgaa aacttcatcc attaaaattt tcaaatatgc ctgttcgttg tcgtcaattg
    12061 acacaaaaat cgaaccgtct ttcataagca gctctttggc gatttctaga cggtttttca
    12121 taaaagtcag ccaagtggaa tgattaaatt tgtcgttgta tttaaaaccg tcattacccg
    12181 tgttatatgg cgggtcaata taaatcagct tcactttgcc tttaaactgc ttggcaagcg
    12241 aatgcagggc aatcagatta ttgcctttga taatgagatt ttcggcgggt gtgccgtctg
    12301 aatgtcgttt gatttcgcca acggcttgtt tgccgtctgc ggtatagcga gagaattttg
    12361 agaatgcttt tgcgtcaaaa agccgatcaa tttcatcaaa agcaagggtt tgattaaaaa
    12421 agatttcttg tctttttcgg gttaatttag tgtataattg gctggctggc tggctggctg
    12481 gctgttattg cgtttaaaat aaatttcttc gccttcctcg gtgctttgtc cgccatttaa
    12541 cacacaatct ttaaacggaa aatccaacac aatatcactg ctgtctttca aaaagcggtt
    12601 gctgtccgtc aaaccaatgc ggttggcgta ttttgtgtag gaattattga cgctgtgttt
    12661 gtccaagaaa aaacggaaat cctgcaattt aaacaccagc acaccattca cttccacaaa
    12721 gaaatggcgt tttaactcat cattactcaa taacaaaccg ataatggtcg ggtcggtttt
    12781 ttccaccaaa tccaacagga tgtttttggc taaaatggtt ttttcttcgt tcgcccatac
    12841 tttttcgtgt ggtagtaggg cttgggttaa ttcggtttga atgtctgtct tcatcgttat
    12901 aaatcctcat cagttatttt aatgtgccat tcttgtgtaa cggcaaatgg ctttgcaaat
    12961 agattctaat aaaatcaatt atttgatgtc tttgctgttt ccgggggcgt tcgcaaagtt
    13021 gcgataagcg gggcgggtgt ccgagtgtgt cgggattttg cggaaatatc aacacattat
    13081 tcaagcacag cctgtatttt taaccgccgt tcaggcggcg gatgccgccg tgttcatcag
    13141 gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccagaccga caaagcggcc
    13201 gttttccgtg taaacgcgta ccggcgtgtc ggaaggcagg tcttcttcga aacgcggacg
    13261 ttgtccgcag tggagcatat ggacggcata atcgtttaaa acggtttggg gaaagtgtga
    13321 aaccaatacg tcgcagggta gcagcaagct gtcgcgttct gtttcgttca aatttgccaa
    13381 ggcctcaagc gtgtggcttt gggcgatggt aaagccggcg gtttcagtgc ggcgcagggc
    13441 ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg tgcggatgta
    13501 ggtgcctttg ctgcaacgta cgtcgatgac ggctttgggc gcgtcaaatt cggcaatatc
    13561 gatggcgtaa acggtaatgt agcgcgcttt gcgttcgatg acgatgcctt tgcgggcgta
    13621 ttcgtacagc ggtttgcctt cgtgcttgag cgcggaaaac attggcggca cttggcggat
    13681 gttgcctgtc agtgcttggc aggccgtctg aaattcggct aaggaaatat cggcgcgggc
    13741 ggtggcaatg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga gtttcagcgt
    13801 ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg cttcaccgaa
    13861 gcagacgggc aaaagtccgg ttgccaaagg gtcgagcacg ccggtatgtc cggctttttc
    13921 ggcatgaaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc cttcgggttt
    13981 gtccaagagc aaaacaccgt tgacggcacg tttttggggt ttgcctgtat tcatggttcg
    14041 atgttcggac ggcatgggca tatccgtcag tcttcaaccg gtttttccgc cgctacttgg
    14101 tcgataaggg cggacaggtt caaaccgcgt tccaaagatt cgtcgtattt gaaatgcagt
    14161 tcgggcgttt tgaacagctt gatgcgtttg gccaattcgc tgcggaggtg tccgcgcgcg
    14221 tgttccagca cttcttccgt aatttcgcgc gcgtcttggt ttaaaatggt gtagaacacg
    14281 gtggcgtggc tgtaatcgcg ggtaacttcg acttcgttga cggtaatgaa gccggcgcgc
    14341 gggtctttca gtccggtacg gacgagttcg gcaagctcgc gcataatttg ttctttgaca
    14401 cggtcttggc gggcatagcc gcgttggggt tttctcatga atatcctcag atttcagatt
    14461 tggtttcaga cggcattttt gccgccgggg cggttgcgaa agggcgtatt atagcctaat
    14521 cggcgggatt ttaaggtaat acattgataa gaacggatat agttattata cttggcaacc
    14581 cgaacggcac gtgccgtttt ctgtaacgca tacattgtaa agaaagccat gtccaacgaa
    14641 aaccgtactt gttccttttg cggaaaatcc aaatcacacg tcaaacattt aattgagggc
    14701 gaaaacgcct ttatctgtga cgaatgcgtc tcaaactgta ttgaaatatt gcatgaagac
    14761 ggaaatgacg gcacgccttc ggaaagtgcc ggaggggagc cggaagaatc cggcaagctg
    14821 cccacgcccg ccgaaatcgt cgccaacctc aacgaccacg ttatcggtca ggaacaagcc
    14881 aagaaagcct tggcggtgtc ggtgtacaac cattacaaac gcctgcgcca tcctaaagca
    14941 ggagctaatg tcgaattgtc gaaatccaac atcctgctta tcggcccgac cggatcgggt
    15001 aaaaccctgc tggcgcaatc tttggcgcgc aaactggatg tgccgttcgt gatggcggat
    15061 gccacaactt tgactgaagc aggttatgtc ggcgaagacg tcgagcaaat cattaccaag
    15121 cttttgggca aatgtgattt cgatgtcgaa aaagcccagc gcggcattgt ctatatcgac
    15181 gaaatcgata aaatttcacg caaaagcgac aacccgtcga ttacccgcga cgtatccggc
    15241 gaaggtgtgc aacaagcctt gttgaaactg attgaaggta cagtggccag cgttccgccc
    15301 caaggcggcc gcaagcatcc gaatcaggaa tttatcaacg tcgataccac caacatcctg
    15361 tttatctgcg gcggcgcgtt tgcaggcttg gaaaaagtca tccgccagcg tactgaaaaa
    15421 ggcggtatcg gtttcggtgc gtccgttcac agcaaggacg aaaatgccga cattaccaag
    15481 ctgttcggca tcgtcgaacc ggaagacttg attaaattcg gtctgattcc agaattaatc
    15541 ggacgtttgc ctgtgattgc aactttagaa gaattggacg aagacgcgct gattaatatt
    15601 ttgaccgagc cgaaaaatgc tttggtcaag cagtatcagg cgttgttcgg tatggaaaat
    15661 gtcgaactgg agtttgaaga aggcgcattg cgttccatcg cgcgtcaggc aatggaacgc
    15721 aaaaccggcg cgcgcggcct gaggtccatc gtcgaacgct gcctgctcga tacgatgtac
    15781 cgcctgcctg atttgaaagg cttgaaaaaa gtggtggtcg gtaaggcagt catcgaagag
    15841 ggcagggaac cggaattggt gttcgagtcc tgatggcaaa caaaaccgct tcggaacgtt
    15901 gaaccacgtt tctgaagcgg ttttttgttt ctgatgccgt ctgaagcctt cccgccttca
    15961 gacggcattt gagttttacg gattgtctga tgtcgcggca aattccgtca gcgcgtgttc
    16021 caaacgtcgc aaaccttccg ccatatcctc atcgttcaac agtagcgaag gcgcgaaacg
    16081 caatacgtcc gcacccgcaa ccaaaatcat cacgccgtgt ttcaaggcgg cggcggtgat
    16141 ttcggatgcg cgtccgcgat aggcttcgtc caacacgcag ccgagtagca gccccatccc
    16201 gcgaacttgt gagaacaagc ccgttttcct gcacaaatcc agcaatgccg tctgaagttt
    16261 ctgcccctgt tcacggacat ggtttaaagt ttcgggcgta ttgatgatgt cgaatgcgcg
    16321 gctgccgacc gcacacgcca tcgggttgcc gccgaaagtc gagccgtgcg tgcccggttg
    16381 gaaggcggcg gcaatctttt ctgtcgccag catcgcgccg atcggaaagc cgcagcccaa
    16441 ggcttttgcc gaactcaaaa tatcgggaac aatgccgtaa tgttcatagg caaacagcct
    16501 gcccgtatgc cccatcccgg tttgaacttc gtccaaaatc aacaacgcat tgtgccggtc
    16561 gcacagacgg cgcgcggttt gcaaatattc cgcagtggcg ggcaggatgc cgctttcgcc
    16621 ctgtatcggc tcgatgatga ccgcgcaggt ctgttcgccg acggcagctt ccagcgcggc
    16681 aatatcgttg aacggaacgt gcgtaatgcc ttgcggcagg ggtgcataat ccttgctgta
    16741 tttcggctga ccgccgacgg acacggtaaa cagcgtgcgt ccgtgaaaac tgttgataca
    16801 ggcgacgatt tcgctttttc ctccgccgaa acggtcgcgg gcgtatttcc tcgccagctt
    16861 caacgccgcc tcattcgctt ccgagcccga gttgcagaaa aaaaccttgt cggcaaaact
    16921 gtttgcaacc aatttttgcg ccaattcctg cgctggacgc gtcgtataga tattggaaat
    16981 gtgccacagc ttgtgcatct gcgcgtttaa agcatcgaca agggcagggt ggcagtgtcc
    17041 cagcgcattg acggcgatac cgcctgaaaa atcaatatat tcacgccctt tcgtatccca
    17101 aacgcggctg cctgaagcgc gttcgggaat catcggggca aaggcgaaat tgggggtcag
    17161 atagttttgc ataattgctc ctgattgcgg ttttgctcgg gattatgcac cgtttttcaa
    17221 agcagataaa gggaaattgc cgacaatatc gggcagcggt aaaaataaac cgaaatattt
    17281 tcaatcggat agatgaaaaa tcaaaaaaat ttcgataaaa tgcttgaggc gcggcagcgt
    17341 tttgatttag aatctcccca atatttttat cttcagacgg catttcatca tgcaaggcaa
    17401 aaaatttaca accgcttatt gctattggta ccacacagac gtgcggtact tttttgcgct
    17461 tgcttgaaat ccatcccata cgcaaaacag gccgctcgaa aacggggcgg cttttttgtt
    17521 accttcacac aggtcgcccg attcggcaac acacgacaga atcaggaaaa tgaccatgaa
    17581 tgcaatgccc caaaccgccg cacaaaacga aaccctgcac acttgtagca ttattgtcga
    17641 tgatgcacag gatttttccc ccatgcttct cgatgcaggg cgggacgaca cacttatcag
    17701 cgaatcgatg attccgcaaa tccgcaccgt tgccgccctg atagccgccg aacggcacga
    17761 cttcagccgc agcagccccg ccgagtttac cgatgccgcc gatttttttg ccgcacggat
    17821 tctcgtattg ggcgtgcgac gcttccatct ggacgtatcg ctgcttcaga ttttgaaaac
    17881 cgccaacagc cgcgcccgcc gctttgccga aaaacaccgc ctgtttttca cacccgcaca
    17941 agccgaattg agcctgaaca aacacaaaaa ccgccgcctg ctgaccgtag aaaccgaaca
    18001 cgaagtcgaa aacaaaggca acccagtcgc caacagcctc gcatttgtcc gcaaattgca
    18061 caccctgccc ctgtaaacat cacgatttgc cggcagaatg cttgccaaaa ttcccgacgg
    18121 cgatacaata aaaccgattt tccacccaaa caggacacac atgaaaaaaa tcatcgcctc
    18181 cgcgcttatc gcaacattcg cactcgccgc ctgccaagac gacacgcagg cgcggctcga
    18241 acagcagcag aaacagattg aagccctgca acagcagctc gcacagcagg cagacgatac
    18301 ggtttaccaa ctgactcccg aagcagtcaa agacaccatt cctgccgaag cacaggcaaa
    18361 cggcaacaac gggcaacccg ttaccggtaa agacgggcag cagtatattt acgaccaatc
    18421 gacaggaagc tggctgctgc aaagcctggt cggcgcggcg gcaggcgcgt ttatcggcaa
    18481 cgcgctggca aacaaattca cacgggcagg caaccaagac agtcccgtcg cccggcgcgc
    18541 gcgtgcagcc taccatcagt ccgcacgccc caatgcgcgc accagcaggg atttgaacac
    18601 gcgcagcctc cgtgcaaaac aacaggcggc gcaggcgcag cgttaccgcc cgacaacgcg
    18661 cccgcccgcc aattaccgcc gccccgctat gcgcggtttc ggcaggaggc ggtaaacccg
    18721 gcgcgccaat gccgtctgaa gagctttcag acggcatttt tgcatttgtt agggacattg
    18781 ttatgttgcc gtttgatttt cagacggcat tttgtttcca agcgtttgat gtcgggatgg
    18841 caattctgat ttacgtgcgt tggcagggtg gcattgtcat gccgccgttg ggttttcaga
    18901 cggcatcata tcgaacagtc aagccgtagg gcgagcattc ttgcccaccg ttgcaagggc
    18961 gggattgggt gggcaggaat gcccaccctg catctgaaac ccttccgtgt cattcccgtg
    19021 caggaaggaa tctagatatt tgaatttacg gaaattttca atcgttgcca aagcggaaaa
    19081 gggtttttat tacagcctaa tcctttgggt atcaaagccg ggcggtgctg aaacattgca
    19141 aaaatgccgt ctgaaactcg aaatcggttt cagacggcat tggtttatgc ggcaggttgg
    19201 ggcttagcgg tctttgtaac ggcgttcgcc tttgcctgca aaagaatcgc cggctttgcg
    19261 tttgcctttg aagccttcgc cggttttttt gaaaccgtct ctcttaaagc cttctttctt
    19321 gaaaccttcg ccgcgcgttt tgccgccgaa gccttctttg ctcggtttat gatcgccgcg
    19381 ccaaccgccg gatttacgat cgccccagcc gcctttgcct ttcggcttgc cgcctgcgga
    19441 tttgcgtttg cgggtcggtt ccatgccttc gatggtcagt tcgggcagtt ttcggttaat
    19501 gtatttttcg attttgtgga ctttgacgta ttcgttcact tcggcaaacg taatcgcaat
    19561 acccgtgcgg cctgcgcggc cggtgcgccc gatgcggtgg acgtagtctt ccgcctgttt
    19621 cggcaggtcg tagttgataa cgtgggtaat ggtcggtacg tcgataccgc gtgcggcaac
    19681 atcggtggca accaaaattt tgcagcggcc tttacgcaaa tccatcagcg tgcggttgcg
    19741 ccagccttgc ggcatatcgc cgtgcaggca gtttgcggcg aaaccttttt cgtacagttc
    19801 atccgcaatg acttcggtca tggctttggt ggacgtgaaa atcacgcatt gatcgatatt
    19861 ggcatcgcgc aagatatgat cgagcaggcg gtttttgtgg cgcatatcgt cgcagtacag
    19921 cagttgttct tcgattttgc cttgatcgtc cacgcgttcg acttcgatga tttcagggtc
    19981 tttggtcagt ttgcgcgcca gtttgccgac cgcgccgtcc caagtggcgg agaacaacaa
    20041 agtctgacgg tcgctcggcg ttgcttccac gatggtttcg atgtcgtcga taaagcccat
    20101 atccaacata cggtcggctt cgtccaaaat cagcacttcc aaacgttcaa aatcaacttt
    20161 gccgctttgc atcaggtcca tcagacggcc cggcgtggcg acaatcagat cgaccggttt
    20221 gctcagggca cgggtttggt agccgaaaga cgcgccgccg acgatgctga cggtgcggaa
    20281 ccaacgcata tttttggcat acgccagcgc gtttttctcg acttgagccg ccagttcgcg
    20341 ggtcggggtc aacaccaaag cacgcgggcc tttgcccggt ttttcgctgc gtttggtcag
    20401 tttttgcaaa gtcggtaaca gaaaggcggc ggttttgccg gagccggttt gcgccgaagc
    20461 catgatgtcg cggccttcca aagcaaacgg aatggcttgc gcctgaatcg gcgtcgggct
    20521 ttcgtaaccc tcgctgctga cggcggacaa aatgttttta tcaaggttca aatcggcaaa
    20581 tttaatagac atggctatcc ttggaagaca acaacgcccg cgtccgaaag gttcagacgg
    20641 cctgaagcag ggaagggtaa cgatacgggg aatgtgaagt tacagggttg tcgcaagcgg
    20701 cttgcttgtg gtttacatcg acggcaacct gcacagatat gctttgcaaa atcaaagcaa
    20761 cagaagaaac gcgcccgtaa gggcggttta ggttggaagg cgatggcttc gtatgacaag
    20821 ataagcgacg gatggtatag ggtttgcccg aaacggtcaa gcaatcgggc gttaattaca
    20881 gtaaaattgc cgttttccac tttattccgc ctgaaaccaa tgaccgacat cacaccgttt
    20941 gccaaccgct tgggcaaaaa catcaaacac cttatgaaat gggcgaaacg caacggcatc
    21001 gaagcctggc gcatttacga ccgcgacatc ccccaattcc cctttgccgc cgatgtttac
    21061 ggcgatcgaa tccaccttca ggaatacgat accggctggc tgatgcggcc cgaagaatac
    21121 gaagcgtggc ttgccgaagt cttggaagcc gtcgctttcg ttaccggttt tgcgcccgaa
    21181 caaatccgcc tcaaacgccg cgaacgccaa aaaggtttgc agcagtacga gaaaaccggc
    21241 aaggcgggcg acgatttcgt catcaccgaa aacggccgca agttttgggt caaccttgat
    21301 aagtatttgg atacggggct ttttttggat catcgaaaca cgcgcaaaaa agtcggcgaa
    21361 acggcggcgg gcaaacgttt tttaaacctg ttttcctaca ccggcagctt caccgtctat
    21421 gccgccaccg gcggcgcggc atccagcgaa accgtcgatt tgtccaacac atatcttgat
    21481 tgggcgaaac gcaattttga attgaacggc atcgatacgg aacgacacaa aatcgtccgc
    21541 gccgacgtgt tccaatatct tcagacggca tatggggaag gcaggcggtt cgacctcatt
    21601 gtgatggacc cgcccagttt ttccaacagc aagaaaatgt ccgacatcct cgacatccag
    21661 cgcgaccaca aaaagctgat tgacggcgcg gtgaagctgc tcgcttcaga cggcattttg
    21721 tacttttcca acaacctgcg cagcttcgtc ttagacgact tggtatcgga acaatacgcc
    21781 gtcaaagaca tttccaaaca atccgttccc gaagatttcc gcaacaaaaa aatccatagg
    21841 tgctgggaaa tccgccacaa atcttaaggg tgcgaaaaag ccgccggaat cttccggcgg
    21901 ctttttcgat tgcgctttat caggcgatag aggcttcgac aaaggcggtc agctgacctt
    21961 ttgccaatgc gccgactttg gtggcgacga cttcgccgtt tttgaacacc atcagggtcg
    22021 gaatgccgcg cacgccgaaa cgggacgggg tggcttcgtt gtcgtcgatg ttgattttga
    22081 ccactttcag acggccttca aattcggcgg caatgtcgtc caaaatcggg gcaatcattt
    22141 tgcaggggcc gcaccacgga gcccaaaagt ccagcaggac ggggatatct gcatttaaaa
    22201 cgtctttttc aaaagcggca tcgctggtgt gtacaatcaa ttcgctgctc attatttttc
    22261 cttttcggtt gggattggca cgatagcgtg caggataggg cttgggcata aaaatttcaa
    22321 gtgggcgggc gggataatag tttttctgta ccgccccaat cgcctttgca aattctaaaa
    22381 aacggttcgg acagtatttt atttctgaaa attactttta ttatcaaaat gtaaggaata
    22441 cgtttgaggc tgtacagaag cgcgcgggcg gtgtatgctc cgtttgaatt gattcttaaa
    22501 gtatgaatgt tcaaaaccaa acccgaaacc ctgtaaaagg aacactgcga tgactgattt
    22561 ttccgtttgg gagaccgccc cctttggtgc aaccgtcgat catatcctgc aacgctacca
    22621 caatgtccac cgtgcacaat ttgaagaatt ggtgccgctg gcgcaaaaag tggcccaagt
    22681 tcatgccgac acctttccgg cggaaattgc cgagctgctt gcctatatgc agaacgaact
    22741 gctgatgcat atgatgaaag aggaaaggat gctgtttccg atgattaatc agggtgtcgg
    22801 acgcggtgcg gcgatgccca tcggcgtgat gatgcacgaa cacgaagaac acgaccgcgc
    22861 catcgcacgg ctcaaagagc tgaccgacaa tttccaaccg ccagaagggg cttgcggaag
    22921 ctggacgcgg ctttacgcgc tggcgaaaga aatggtggag gatttgaacg accacattca
    22981 tttggaaaac gatattttgt ttgcccgcgt actcgattct taagggatag gagtcggata
    23041 tttccatgcc gtctgaaata cggttttccg tttcagacgg catttccgtg tttcagccca
    23101 gcaattcggg cggaatgcgg catacgggaa tcgggttgat tttgtgctgc atggcgcggt
    23161 gaagtcgcgt atagatttct agaacttcgc gctgccgccc ttcaaaatct tcgggtttgc
    23221 gcgtgccgta cacgcccatt gcccactcca gttcgggata gcttgcgccc atctgttctt
    23281 cgtcggtgcg ttccgtatcc cacaggccgt cggtcggcgg ggctttttga atcgcctcgt
    23341 ccacgcccaa tgcttcggca agccggtaaa cctgcgtttt ggtcaggtct gcaatcgggc
    23401 tgatgtccac gccgccgtcg ccgtatttag taaaaaagcc cacgccgaaa tcttcaatct
    23461 tattacctgt ccccgtaacc agcagtccgt gtatctgccc gtagtagtac agggtcagca
    23521 tacgtaggcg gcttctggcg ttggcgaggg aaagcggctg actgtcaaat gccgtctgat
    23581 gagcaccgac ggtttgttca aaggtctgga aggtgtcggt cagatcgacc gtttgcgcgc
    23641 ttacattggc atattgccgt tgcagattgc ggatgtgcag ccttgcccgc tcaagctggc
    23701 cggggtgttg gcgtatcggc atatccagaa gcagcgtggg gcggccggtg cgggcggcga
    23761 gtgcggagac gacggcggaa tcgatgccgc cggaaacgcc cacgacaaac ccttttgcat
    23821 ttgcccgggc ggcgtattcg tccaaccatc gggcgatatg tgtgataact gcctgcgtat
    23881 ccatttttcc tcctgtcgaa aatatccgca tcatagcctg ccggcggtgc aatttgaata
    23941 gattgcaggc aggttgatga atatttttcc cattccccgc ttaaaaaagt catacgggaa
    24001 aaggcgggcg gcaagatggc gcgtgttata atattgttga caatttaggc ggcccggtgc
    24061 cgccttaggc ttcaaaacat attcatattt tttactattt gattgattcg atgtctgatt
    24121 tttttcattc tgacgtgttg tccgtttccg agttgaacgc atttgccaaa agtcttttgg
    24181 aaaaccacct tgccggtttg tggattgccg gcgaagtatc caacctgacc cgtgccgcca
    24241 gcgggcatta ttatttctcg ctcaaagaca gccgcgcaca ggtgcgttgc gcgatgttca
    24301 agggtgcggc ggtgcgtttg gcgaagcctt tgaaagaagg cgaccatatc gaggtatcag
    24361 gaaaaatcag tatttatgaa gcgcggggcg aatttcagat taccgtaaac gaagtacggc
    24421 tcaaaggttt ggggcagctt tacgaagcct atgagcggtt gaaggcgcag ttgcaggcgg
    24481 aaggcgcgtt tgcggcggaa cgcaagaaac ctttgcccgt ccgtccgcaa tgtatcggca
    24541 tcgtaaccag tctggcggcg gcggctttgc gcgatgtcgt tactaccttg aaacgccgcg
    24601 cacccgaaat ccccgttatc gtttatccga cacccgttca aggcgcaggc agcgaattgc
    24661 aaattgccca agcgattaaa accgcctcgc aacgcgccga atgcgatgtg ttgattgtct
    24721 gtcgcggcgg cggcagcatt gaagacttgt gggcgtttaa cgaagaaccg gtcgtgcgcg
    24781 ccatcgaatc ctgcacggtt ccggtcgtca gcggcgtagg ccacgaaacc gatttcacgc
    24841 tcgccgattt cgtcgccgat atgcgcgcgc ccacgccgac cggtgcggcg gaactggtca
    24901 gccccaaccg ccaagaatcg ctgcaccgcc tcgcccaagc ccaatgccgt ctgaaaaccg
    24961 ttttggaaca acgctatttc gatgccagcc aaaaactcga ctggctggcg cggcaaatcc
    25021 gtcatccgcg ccaaaaactc gacgaacagc gcgcttcaat cggcaagctg gcgcaaacgc
    25081 tgtcttactc gatgacgcaa aacgtccgta cccacaccgc ccgtttcgaa cgccaaaccc
    25141 aagccctgaa acattgccgt cctgatgttt ccgtttgcaa aaaaaacatc gaccgctttc
    25201 agacggccct gtcgcacgcc ttccaccaac tgcttaccca ccgccgccaa agcctgactg
    25261 cccaagccgc attgctcgaa gccgtttcgc cgcagcacat tctggagcgc ggcttttccg
    25321 tcgtcaaaaa cacacgcgga caagtcatcc gcaacgccga tgttttgaag caggggcaga
    25381 agctgcacat tatcttcgcc gacggcgaaa ccgacgtgcg cgtaagcaaa gaacaagggc
    25441 agcaggattt gttcgattgc atttaaaccc aatcggaaaa accgctcccc cgccgccgtc
    25501 attcccgcga aagcgggaat ccagtccctt gaccttaaga taatctttaa ttcgtcattc
    25561 ctgaaaaagc gggaatccgg aagcgaaaag ccgcaggaat ttgtcggaaa aaacagaaac
    25621 ctttccgtac acccgaaatg tttcagacgg cgcatcttgg ggcggataaa aacccgaccc
    25681 gatatttttt tataattttc aataagtttc cattgggcaa atgaaatttt atttttcaac
    25741 tgacagaaag aggatgttat gaagttttta gaccgtgagg caagcattgc caagccgggt
    25801 ttcaaccgtt ggctcgtacc gcccgcagca ttggcggtac accttgccat cgggcagatt
    25861 tacgcctatt ccgtattcaa cgcgccgctg accaaactta tcggcataac cgaatcggcg
    25921 gccggagatt ggaagctgac gaccgtaggt tggattttca gtatcgcact cgcgatgctg
    25981 ggcgcgtcgg ccgccctgtt cggtacgtgg atggaacggg tagggccgcg caaagccata
    26041 tttgccgccg cctgctgctt cagcctgggc ttcttcgtat ccgcattcgg cgtgcgtacg
    26101 cacaacctct tcctgctcta tttgggcaac ggcgtcatcg gcggcgtagg cttgggtttg
    26161 ggctacatcg ggccggtttc cacactgatg aaatggtttc ccgacaagcc gggtatggcg
    26221 accggtttgg cgattatggg tttcggtggc ggcgcaatgc tggcctcgcc gctttccgta
    26281 tcactgatga acaccttttc aaacgctgct tcggtcgggg ttgccgaaac cttcgccgta
    26341 ttgggtctgc tttacctcgc actgatgatg ttcggcgcgt ttaccatccg cgtacctgcc
    26401 gacggctgga aacctgaagg ctataccgcg ccgaaaacgc aaaacaagct ggtcagcagc
    26461 aatcatgtca acgtgtccca agccatgaaa acgccgcagt tttggctgtt gttctgggta
    26521 ttgtgcctga acgtaactgc cggcatcggc gtattgggtc aggcatccgt gatgattcag
    26581 gaactctttt ccgaaacctc cataggcagg caggcagcag tcggcgcagg tgcggcggca
    26641 ggcttcgtca gcctcctgag cctgtttaac atgggcggac gctttttgtg gtcgagcgtt
    26701 tccgacaaaa tcggacgcaa aaacacctac accatcttct tcgtactcgg ttcgctgctg
    26761 tatttcgccg ttccctccat cggcgagggc ggcagcaaag ccctgttcat catcggcttt
    26821 tgcgtgatta tttccatgta cggcggcgga tttgccgcca ttcccgccta cctgaaagac
    26881 ctgttcggca cctatcaggt cggcgcgatt cacggacgca tcctgctggc ctggtcaacc
    26941 gccgccgtca tcggcccggt actggtcaat tacatacgcc aaagccaaat cgacagcggc
    27001 ataccggcgg cacaggcata cagcgtaacc atgtacatca tggcgtgcct gctgattatc
    27061 ggtttgttgt gcaacctcgc cgtcaaatcc gtacacgaaa aacaccacga aaaagacatc
    27121 aaaaccgccg cacacagcgg caaccccgat gacgaaaccg ccatatccga cgcttacctt
    27181 gtgggcgaaa aagtttccgg cggcggcata tcggtctggt ggcgttgggc attggccgtc
    27241 ataccgctcg cctacggcgt ggtgatggtc tttgtcaaag cactcgacct tttctcctga
    27301 aaacgcccga ataaaatgcc gtctgaaaca gcagatgttt cagacggcat ttttgtgttt
    27361 tggcgggttt aggctttttt gcggcggcgt tcgcccggca gcagcatgtc cgcccatttg
    27421 atgatgttgg cgacttcggc ggggttgagt tcgtagaact gcccgcgttt gaggcggttg
    27481 ggcagtccga tgggaccgaa gccgatgcgc acgaggcggc tgacggtgag tccttggctt
    27541 tcaaaaatgc ggcgcacttc gcggttgcgg ccttctttaa tcacgacgtt gtaccatttg
    27601 ttcgcgcctt cgccgccttg ttcgcggatg cgttcgactt ttgccaagcc gtcttcgagc
    27661 atcacgcctt cttcggtgag gacgcgcatt tgttcgccgg tcagcccgcc caagacgcgc
    27721 acggcgtatt cgcgttcgac ttcgaagctg gggtgggcga aacgttggac gagttcgccg
    27781 gaggtggtaa gaatcagaag tccgctggtg ttgatgtcca agcgtccgat ggcgacccag
    27841 cggctgctgg cggcctgcgg caggcggtcg aatatgctga cgcggccttg cgggtcgtca
    27901 cgggaaacga tttcgccttc ttgtttgtaa tacaggatga tgcgcggcag gcggtccgcc
    27961 catttgagct tgatgatgct gcctttgacg gtaacgtggt cgtcgggggt aactttgtcg
    28021 cccagttgcg cggttttgcc gttgaccgtt atccagccgt tggtaatcca ttcttccatt
    28081 tcgcggcgcg agccgacgcc ggacgcggca agcacttttt gcaggcgttc gggttccatg
    28141 cgcgacaggt cgctgcggcg ttctttcaaa tcgcgcgcgt gttccataat tttttggttg
    28201 ggattgcgga cgacgagttt tttggctttg gcggcgcgct gtttggggac gttttgaggt
    28261 ttgatgccgt ctgaagcttt ttgtccgtaa ggttgggaag cggttttgcc cgtttcatct
    28321 ttgggacggg ctttgctttt gaacggtttg gcggttttct tggcagacgg ggctgcgccg
    28381 tcgcgccatt ggcgtttgct ggtgggctgt ttggacatca tttttcctaa ctcgcctgat
    28441 aaggcggttc ttctgcggcg tgtgcggcag gacgggttga taaaatgccg tcctcggtcg
    28501 gacggttcga tattgtacct gatgcggcgg ttttgcggat gaaatcgtcg ctgcggcttg
    28561 gggtcggggc aaagtttgtg cgacggttga aatcggtttg cgtgcgggag tagaatcttt
    28621 caaatgataa gaggagcagt aaatggattt gcacaatatt cgggaagatt acagcaaacg
    28681 ggaattgtcg gaagccgatt gcgccgataa tccgatcgag cagttcgagc ggtggttgga
    28741 cgaggcggta cgcgcacagg tcaacgagcc gacggcggtc aatgtggcgg cggttgacgg
    28801 acgcggcagg cccaacagcc gtatggtgct gctgaaggaa gttaattctg aaggttttgt
    28861 tttctttacg aattatcaca gccgcaaggg gcgttcgctg gatgctcatc catttgcggc
    28921 gatgacgttt ttttggccgg agctggagcg tcaggtgcgc gtggaagggc gtgttgaaag
    28981 gctggcggag aagttgtcgg acgaatattt tgagagtcgc ccgtatcaga gccgtttggg
    29041 tgcgtgggca agtgcgcaga gcgaggttat tccgaacaag gcggtattgg tggcaaaggc
    29101 ggcggcggtt ggactcaaac atcctctgca tgtgccgcgt ccgccgcatt ggggcggcta
    29161 tattgtgatt cccgatttgc ttgagttctg gcagggcaga ccgagcaggc tgcacgaccg
    29221 catccaatac cgtttgctgg atggtggctg gatacgcgag cggctgtcgc cttgaacagg
    29281 aaaaaacata tgccgtctga aaaccggaat cgggtttcag acggcatttt ttcacgggga
    29341 aaacacggac gggaagtttt ttgtgttctg aacggaaagc tcgaatcctc tttataccgg
    29401 ggaattatga tgcggacggc gtgaatgcgt cggagaaaaa caagttttcc ggcagcttat
    29461 gctgttgcac aaacagattc tttgtttgtt cggtcatggc cggagaaccg caggcaaata
    29521 cttcgtattc cgacaggtcg gggtggtctt gtgccgcgat gtcttgtacg tgaccatttc
    29581 ttccctgcca gccctctccg gggcgggaca atacgggggt gaagcaggcg tttttcagac
    29641 ggcatgccaa cccttgtgct tcttcgaggg catacaaatc atcctgatga cgcgcgcccc
    29701 agtagaaatg gacggcgcgg ttgctgcctt ggcggataag gtcgagcagg atgctgcgga
    29761 tgggggcgta gcctgtgccg gttgccagca ggatgacggg tttgccgctg tcttcctgca
    29821 aggtaaacga accgagcggg cctttaacgc ggacgatgcc tttttctttg actttgggtt
    29881 cgctgccgaa aatcatttcc gagcagacac cgttttcgtg cctgcggatg tgcagttcca
    29941 aaatgccttc ttggtcgggt aaattggcga tggagtagct gcggctgacg ttgcccggca
    30001 gcagtaaatc aatgtattgc ccggcgtaga aggcaaacgg cggggctttg ggcagggcaa
    30061 gtttcaggag ggcgacatcg tgtttgaaaa taatactttc gatgcgtgcg ggcagggtgc
    30121 ggacgggtag ggcatcggct ttgtagccgg ggatgttgat gttgatatcg ctttgcgcag
    30181 tggtgcagca catcaaaatc ttgccttgcg ctttttctgc ttcggataaa gcctgttccg
    30241 agtgtccgcc catttgaata tcgccgctga ccagttcggc tttgcattgt ccgcagacac
    30301 cgcttttgca ggaatggggc aggttgaggt tttgacgggc ggcagcggtc aaaacggttt
    30361 cgccgtcgtt ggcggcaaag gtggtttggt cgggcagggt aacggtgtgg ttcatgtctt
    30421 ggtatggttt gagcagatta
//
LOCUS       DAWISC010000005        30119 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWISC010000005 DAWISC010000000
VERSION     DAWISC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.005"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(65..904)
                     /locus_tag="WIW87_000152"
     CDS             complement(65..904)
                     /locus_tag="WIW87_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ5173375.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WIW87_000153"
     tRNA            1189..1264
                     /locus_tag="WIW87_000153"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WIW87_000154"
     CDS             complement(1329..2522)
                     /locus_tag="WIW87_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ5173376.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     NRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WIW87_000155"
     CDS             complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WIW87_000155"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ5173377.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACRTAEGLGSDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3778..4878
                     /gene="aroC"
                     /locus_tag="WIW87_000156"
     CDS             3778..4878
                     /gene="aroC"
                     /locus_tag="WIW87_000156"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ5173378.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4970..5395
                     /locus_tag="WIW87_000157"
     CDS             4970..5395
                     /locus_tag="WIW87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ5173379.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5469..7454)
                     /gene="parE"
                     /locus_tag="WIW87_000158"
     CDS             complement(5469..7454)
                     /gene="parE"
                     /locus_tag="WIW87_000158"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ5173380.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     ASDEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7521..8045)
                     /locus_tag="WIW87_000159"
     CDS             complement(7521..8045)
                     /locus_tag="WIW87_000159"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ5173381.1"
                     /translation="MLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            8211..9506
                     /gene="serS"
                     /locus_tag="WIW87_000160"
     CDS             8211..9506
                     /gene="serS"
                     /locus_tag="WIW87_000160"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ5173382.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9584..10582)
                     /locus_tag="WIW87_000161"
     CDS             complement(9584..10582)
                     /locus_tag="WIW87_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ5173383.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10707..11783)
                     /gene="prfA"
                     /locus_tag="WIW87_000162"
     CDS             complement(10707..11783)
                     /gene="prfA"
                     /locus_tag="WIW87_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ5173384.1"
                     /translation="MKPSILEKLQQLSDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLEEITNALIAEHQAELLAAMGD"
     gene            complement(11888..12658)
                     /locus_tag="WIW87_000163"
     CDS             complement(11888..12658)
                     /locus_tag="WIW87_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ5173385.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAEMPSEHGQIRIALGIHPWFSDGIAERDFVRLETMLAHYPQAWVGEIGLDFY
                     DKTQTSQQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12666..13658)
                     /locus_tag="WIW87_000164"
     CDS             complement(12666..13658)
                     /locus_tag="WIW87_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ5173386.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGTLAEWDETRGWHNLRLPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEDVYKELVRLKLV"
     gene            complement(13940..14623)
                     /locus_tag="WIW87_000165"
     CDS             complement(13940..14623)
                     /locus_tag="WIW87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5173387.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILRFKPIARIMTPKRYEQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFIIMDGLAALISVPIWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14814..16148)
                     /gene="glmM"
                     /locus_tag="WIW87_000166"
     CDS             complement(14814..16148)
                     /gene="glmM"
                     /locus_tag="WIW87_000166"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ5173388.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQQWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIAAAIGGI"
     gene            complement(16278..17135)
                     /gene="folP"
                     /locus_tag="WIW87_000167"
     CDS             complement(16278..17135)
                     /gene="folP"
                     /locus_tag="WIW87_000167"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ5173389.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGVYSQNAQTA
                     LAHAEQLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGIDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17275..18168)
                     /locus_tag="WIW87_000168"
     CDS             complement(17275..18168)
                     /locus_tag="WIW87_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ5173390.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18259..20373
                     /locus_tag="WIW87_000169"
     CDS             18259..20373
                     /locus_tag="WIW87_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ5173391.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGTPAIRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GSWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20437..21915)
                     /gene="ubiD"
                     /locus_tag="WIW87_000170"
     CDS             complement(20437..21915)
                     /gene="ubiD"
                     /locus_tag="WIW87_000170"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ5173392.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPISPYLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVNDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21935..22450)
                     /locus_tag="WIW87_000171"
     CDS             complement(21935..22450)
                     /locus_tag="WIW87_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ5173393.1"
                     /translation="MYTCTSCGENHEEMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22457..22936)
                     /locus_tag="WIW87_000172"
     CDS             complement(22457..22936)
                     /locus_tag="WIW87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173394.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23171..23416)
                     /locus_tag="WIW87_000173"
     CDS             complement(23171..23416)
                     /locus_tag="WIW87_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5173395.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23428..23688)
                     /locus_tag="WIW87_000174"
     CDS             complement(23428..23688)
                     /locus_tag="WIW87_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5173396.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23685..24443)
                     /locus_tag="WIW87_000175"
     CDS             complement(23685..24443)
                     /locus_tag="WIW87_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5173397.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPEMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24436..25191)
                     /locus_tag="WIW87_000176"
     CDS             complement(24436..25191)
                     /locus_tag="WIW87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEZ5173398.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFADGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25346..25879
                     /locus_tag="WIW87_000177"
     CDS             25346..25879
                     /locus_tag="WIW87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
                     /protein_id="HEZ5173399.1"
                     /translation="MPVFASLPPQYMDTLPECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25907..26635
                     /gene="fabG"
                     /locus_tag="WIW87_000178"
     CDS             25907..26635
                     /gene="fabG"
                     /locus_tag="WIW87_000178"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ5173400.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26629..27879
                     /locus_tag="WIW87_000179"
     CDS             26629..27879
                     /locus_tag="WIW87_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ5173401.1"
                     /translation="MLNTRRVAVTGIGGITAFGRDWQSIQAAFKAEKNAVKYMDWHER
                     FPELEAQLGAPIENYAPPKHWTRKQLRSMGRVSYLCVDAAEQALADAGLLGDESITDG
                     RMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAANIGIFFGLKGRI
                     IPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDSLYAASRRNGEP
                     EKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGANSDAYHISTPR
                     PDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHNDNMESRAVAAVFGNNTPCTST
                     KPQTGHTLGAADAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPNLPAINLTGSGSRW
                     ETEKRITASSSFAFGGSNCVLIIG"
     gene            27933..28008
                     /locus_tag="WIW87_000180"
     tRNA            27933..28008
                     /locus_tag="WIW87_000180"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27966..27968,aa:Ile,seq:cat)
     gene            28083..28841
                     /locus_tag="WIW87_000181"
     CDS             28083..28841
                     /locus_tag="WIW87_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ5173402.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28842..29906
                     /locus_tag="WIW87_000182"
     CDS             28842..29906
                     /locus_tag="WIW87_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ5173403.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT         6962 a         8479 c         7823 g         6855 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa accggcattg ccgcctttgg ccggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaaca atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaaccttg cgttttccgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagagaccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatt ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacgg tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctta agccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaatccttg gtaggcaatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgccgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccgac agtggcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcctt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg caggaagtcc gccccgactt tgagcgcgcc cgagccgccc
     2221 aaggtttgta cggtaacgat gcgcccttgc gcaagcgcgg gattgtcttt gccgaacaat
     2281 aaatgctgca ccgcgctgcg gtaagtgtcc aagccttcca tcggcaggta gggcgacggc
     2341 gcaggcgcgg cggcacgggc ggtttcggct cggctcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcggggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcggg tcgccaggat agtattcgat gtgtcggtac
     2521 atagtcctta cctcttgctt tttcaaagga ttttcttttt caacaataca ccactttcga
     2581 tatggtgcgt aaacgggaat tggtcgaaca gggcggcacg ttcgaccgca tgggtttccg
     2641 ccaaggtgtc caaattggcg cgcaacgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gacggaggtt ttggagattt
     2941 cggttgccaa aacctgtcgg aaatatcggg acagcggcag ggtgaaattg ccgtttccgc
     3001 aatacagttc gagcaggtcg ctgcccaagc cttccgccgt gcggcacgcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccaata acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc agcatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtggacag aaattcgacg gcataccagc gttttttgag ttcgggggat tgcgcggcgg
     3361 cggcgatcag ctcgggcatg aggcggttga cagcctcgga agctgcttca aaacggtcgc
     3421 agcgtatcat gcttgcgccg ctggctttct gccctttttc aaacatggca taaaacattt
     3481 ccccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tagtcttttt
     3601 taccttgaag ctgctgcgtg tagtcattca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat ttgcctcgcc gcacgcaaac tctttgactg ccgccccttc aatgacggac
     3721 gggcttttgt gctaaaatcc gccatctttc cacactatac cgataaaggg aaaaatcatg
     3781 gcaggcaaca ctttcggaca actcttcacc gttaccacct tcggcgaaag ccacggcgcg
     3841 ggtttgggct gtatcatcga cggctgcccg cccggcttgg aattaagcga agcggatatc
     3901 caatttgacc tcgaccgacg caaacccggc accagccgcc acgttaccca acgccgcgaa
     3961 gccgaccaag tcgaaatcct ctccggcgta ttcgaaggca aaaccaccgg cacgcccatc
     4021 gccctcttaa tccgcaatac cgaccagcgc agcaaagact acggcaacat cgccaccagc
     4081 ttccgccccg gccacgccga ctatacctat tggcacaaat acggcacgcg cgactaccgc
     4141 ggcggcggca ggagctccgc ccgtgaaacc gccgcccgcg ttgctgccgg agccgttgcc
     4201 aaaaaatggt tgaaagaaaa attcggcacg gaaatcaccg cctacgttac ccaagtcggc
     4261 gaaaaagaaa tccggtttga aggctgcgaa cacatttccc aaaatccttt ttttgccgcc
     4321 aaccatagcc aaattgccga gctggaaaac tatatggaca gcgtgcgcaa atccttggat
     4381 tccgtcggcg cgaagctgca tatcgaagca gccaatgtcc ctgtcggctt gggcgaacct
     4441 gtttttgacc gcctcgatgc cgaaatcgcc tacgcgatga tgggcatcaa cgccgtcaaa
     4501 ggcgtggaaa tcggcgcagg ttttgacagc gtaacgcaac gcggcagcga acacggcgac
     4561 gaactgaccc cgcaaggctt cctgtccaac cactcaggcg gcatcctcgg cggcatcagc
     4621 accgggcaag acatccgcgt caatatcgcc atcaaaccca ccagctccat cgccaccccg
     4681 cgccgcagta tcgacatcaa cggcaacccc atcgaactcg ccacgcacgg caggcacgac
     4741 ccctgcgtcg gactgcgctc cgcgccgatc gccgaagcca tgctcgcgtt agtcctcatc
     4801 gaccacgccc tgcgccatcg cgcgcaaaat gccgacgttc aggttaatac gcccgacatt
     4861 accctttcaa acaaataaaa atttagccaa aacacagact ttataataga atatcgagca
     4921 ttgccgtgca gccttgacgc acgggtttgt ttagaggaat acaaccgaaa tgacacaaga
     4981 aaccgctttg ggcgcggcac taaaatccgc cgtccaaact atgagcaaaa agaaacagac
     5041 cgaaatgatc gccgaccaca tctacggcaa atacgatgta ttcaaacgct tcaaaccgtt
     5101 ggcgctcggc atcgatcagg atttgattgc cgctttgccg caatacgatg ccgcactgat
     5161 tgcacgcgtc ctcgccaacc actgccgccg tccgcgctat ctgaaagcct tggcgcgcgg
     5221 aggcaaacgt ttcgatttga acaaccgttt caaaggcgaa gttacccccg aagaacaggc
     5281 gatcgcgcaa aaccatcctt ttgtgcagca ggctttgcaa cagcagtccg cccaagctgc
     5341 cgccgaaacg ctgtctgttg aagccgaagc agccgaatct tccgcagcag aataaatccc
     5401 caaacgaaat gccgtctgaa aaccgatttg gtttcagacg gcattttttc gtatgcggca
     5461 atcacggttc aaatatccaa ttccgccgta tcgccttcgc gttccatcca agcgcggcgg
     5521 gcggcggctt cgcctttgcc catcagtttg acgaagatgt cgcgcgtctc gtcatctgca
     5581 ccttctggga tttgtacctg caacaggcgg cgggtgtcgg ggtgcatggt agtatctttg
     5641 agctggtcgg ggttcatctc gcccaagcct ttgaaacggc tgatggaata ggcggtttct
     5701 ttaacgcctt ctttttgcag ccgctccaaa atgctgtcga gttcgttttg gtcgagggcg
     5761 tagaatttgc gggcaggttt gctcttacct tgtgcgttga catcgacgcg gaacagcggc
     5821 ggctgggcga cgtagatgtg tccgtcggca accagtttcg ggaagtggcg gtagaacagg
     5881 gtcagcagca aaacttgaat atgcgagccg tccacgtcgg catcggacag gatggcgatt
     5941 ttgccgtagc gcaggccgct taaatcggga tggtcgttaa taccgtgcgg atcgacgccg
     6001 atggcgacgg aaatgtcgtg gatttcggcg ttgccgaaga gttggtcggg gtggacttca
     6061 aagctgttga gcactttgcc gcgcaggggc aggatggctt gggtggcttt gtcgcgggcg
     6121 agtttggctg agccgccggc ggaatcgcct tcgacgagga agagttcgtt ttcgcggatg
     6181 tcttcgcttt cgcagtcggt cagcttaccg ggcaggacgg cgacgccgct gccttttttc
     6241 ttttcaatct ttttaaccga acgcatccgc gcctgtgctt ggcggatggc gagttcggcg
     6301 atttttttgc cgaagtccac gttttggttc agccacaatt ccaaagggtc gcccgatacg
     6361 gtggcgacga gtttcagcgc gtcgcggttg gtcagcttgt ctttggtttg accttggaac
     6421 tgcgggtcga ggacgcgggc agagagaacg aaggcggttt ttccgaacac gtcgtcgctt
     6481 tgcactttaa cgccgcgcgg caagaggttg tgcagattga tgaagttgtt gactgcgttg
     6541 aacacggctt gtttcaagcc tgcttcgtgc gtgccgccca gcggggtggg gatgaggttg
     6601 acgtagcttt cgttggcgca cgagccttct tccagccaag tcagggcaaa cgccgctcct
     6661 tcgccgatgc tgaaatcgcc gttgtgttcg tctgaaatgt agttttcgca agagaacagc
     6721 ggtacggctt cctgcgcgtc ggcaatcagg tcggtcagat agcttttcag gccgtcgggg
     6781 taatgccagg tttgggtgtg cgcttcgtct tcgcctttga ccggacgggt cagggaaacg
     6841 cgcacacccg gcagcagcac ggctttggca cgcagcaggc gttcgagttc gggaatgctg
     6901 taattcgggc tttcaaaata tttgccgtcc ggccagacgc gcacttctgt accgctgtct
     6961 ttgacggcgc atttgcccac ttgtgccaac ggttcgacca cgtcgccgcc ggcaaacacg
     7021 atgcggtgga ttttgccttc gcgtttgacc gttacttcaa ggcgggtgga aagggcgttg
     7081 gtgacggata cgcccacgcc gtgcaggccg cctgaaaagg catacgcgct gcctccgtct
     7141 tttttgttga acttgccgcc tgcgtgcaga cgggtgaata cgagttcgac tacggatacg
     7201 ccttcttcgg gatgcaggcc gacgggaatg ccgcgcccat tgtcgtgcac ggaaagcgaa
     7261 ccgtcttcat gaatttgcac gtcgattgca gtcgcgaaac cgcccaacgc ttcatccgac
     7321 gcgttgtcga tgacttcttg gcagatatgg gtcgggctgt cggtgcgggt gtacataccg
     7381 ggacgttctt tgaccggctc caagcctttg aggacggtga tgctggattc gctgtattgg
     7441 ttgtttttag ccatgggaat aatctgaaag taagaaaaac aacgctttca gacggcctga
     7501 aagcgttgcg ttccgttgtt ttagcggttg tcggaagatt ggcgggcggc aaagtcttca
     7561 taactttcca taccgcgcag gaagcgggaa gagagttctt tcaacgcccc caaataaacg
     7621 tcgcgtttga aatcaatcac ttcgtcaacg ggtgcccaat attgatgcca acgccagccg
     7681 tcaaattcgg ggtggcgggt ggcgcgcagg ttgacatcgc aatctcggcc ggtcaggcgc
     7741 aggagatacc aaatctgctt ctgtccgcga taagagccgc gccattcgcg gcgtacccag
     7801 ttgttcggca cgtcataacg cagccagtcg cgcgtgcggc cgataatttt gacgtgttgc
     7861 ggcaaaagcc cgacttcttc gtacaactcg cggtacatgg cggtttcggg gctttcgccc
     7921 ggcttgatgc cgccttgcgg aaactgccaa gaatgttcgc gcacgcgctt accccaaaag
     7981 acttcgttgc ggttgttgat taagatgata ccgacattgg ggcgatagcc ttccctgtcc
     8041 aacacggtgt cgccctccgt taaattcaat cttgggattt tcccacaaat caggcggttt
     8101 tgacaaatca gacggcatgg cggtacgcgt gccgaaacac ggggggattt gggaaaatat
     8161 cttaaatttg gtttacaata atgtatttca aattattcgg gaatcagacc atgttagata
     8221 tccaattgct ccgcagcaac accgccgccg ttgccgaacg gcttgcacgg cgcggttatg
     8281 actttgatac cgcacgtttt gacacactgg aagaacgacg caagtccgtt caggtgaaaa
     8341 ccgaagaatt acaggcctcg cgcaacagca tttccaaaca aatcggcgca ctgaaaggtc
     8401 agggcaaaca cgaagaagcg caggcggcca tgaatcaggt tgcccaaatc aaaaccgatt
     8461 tggaacaggc tgccgccgat ttggatgccg ttcaaaaaga attggacgca tggttgttga
     8521 gcatacctaa cctgccgcac gaaagcgtac ctgccggtaa agacgaaacc gaaaacgtcg
     8581 aagtccgcaa agtcggcacc ccgcgcgaat ttgactttga aatcaaagac catgtcgatt
     8641 tgggcgaacc tttgggtttg gattttgaag gcggtgcaaa actctccggc gcacgattta
     8701 ccgtgatgcg cggacaaatc gcccgtctgc accgcgcctt ggcacagttc atgctggata
     8761 cgcacacgct gcaacacggc tacaccgagc attacacgcc ttatatcgtt gacgatacga
     8821 cgctgcaagg tacgggccaa ctaccaaaat ttgcggaaga tctgttccac gttacccgtg
     8881 gcggcgacga aaccaaaacc acccaatacc tgattccgac agccgaagtt accctgacca
     8941 ataccgttgc cgacagcatt atcccgtccg aacaactgcc gctgaagctg accgcgcatt
     9001 cgccctgttt ccgcagcgag gcgggttcgt acggcaaaga cacgcgcggt ctgattcgcc
     9061 agcaccagtt cgacaaagtg gaaatggttc aaatcgttca tcccgaaaaa tcatacgaaa
     9121 cgctggaaga aatggtcggc catgccgaaa acatcctgaa ggctttggaa ctgccctacc
     9181 gcgtgattac cctgtgtacc ggcgacatgg gcttcggcgc ggcaaaaacg tatgacttgg
     9241 aagtttgggt gccggcgcaa aatacctacc gcgaaatctc aagctgctcc aactgcgaag
     9301 atttccaagc ccgccgcctg aaggcgcgtt tcaaagacga aaacggcaaa aaccgcttgg
     9361 tacatacttt gaacggctcc ggcttggctg tcggcagaac gctggtcgcc gtattggaaa
     9421 accatcaaaa cgccgacggc agcatcaaca tccctgccgc actgcaaccg tatatgggcg
     9481 gtgttgccaa gttggaagtc aaataagttt gcaggctgcc tgaacgtcaa atgccgtctg
     9541 aaacctgttt cagacggcat ttcctttaaa cttttaaaac acgtcagccg tcggcacgaa
     9601 ccgcattgcc gcaatcgccg gtctgtccga cctcgcggat attggacagc gtaacttccg
     9661 aaatattacc caacgcctct tccgtcaaaa atgcctgatg gccggtaaac agcacattat
     9721 gacaagacga caggcggcgg aacacgtcgt cggtaatcac atcgttggat ttgtcttcaa
     9781 aaaacagctc gcgctcgttc tcgtacacat ccatgcccaa tgcgccgatt ttccggcgtt
     9841 tcaacgcctc aatcgcggcg gcactgtcaa tcagcccgcc ccggctggtg ttgataatca
     9901 tcacgccgtc tttcattttg tcgaacgccg cttcgttcag catatagtgg ttttccggcg
     9961 tggcggggca atgcagcgtg atgatgtccg accgggcata cagctcgtct aaatccacat
    10021 atttgccgcc gattttttcc gcttcggggt tgcaaaacgg atcgtaagcc agcaggttca
    10081 tgccgaaacc ctttaaaatc cgcatggttg caataccgat tttccccgtg ccgataacgc
    10141 ccgccgtttt gccgtacata ttgaaaccgg tcagaccttc cagcgaaaaa ttcgcatcgc
    10201 gggtacgctg ataggctttg tggatacggc ggttcaacgt cagcatcaga ccgaccgtat
    10261 gttccgcaac cgattcgggc gaataggcag gcacgcgcac gactttcaag cccaactctt
    10321 cagccgcctt taaatccaca ttattgaagc cggcacaacg caacgccaca gttttcacgc
    10381 caatttgcgc caatttttcc aacacgggcc ggctgccgtc gtcgtttaca aaaatacaga
    10441 ccgcttccgc gccttccgcc attttcgccg ttttcgcatc cagcataaaa tcaaaaaact
    10501 ccagctcgaa gccgaaatgc cggttggcgc gggtaaaatg ttcgcggtca tagcttttcg
    10561 taccgtaaat cgcaatcttc atcaatatgt ccagttgtcg tctatggttg agaaacggca
    10621 taatacactg aattcaaaca aatcagtaga atatggtgga ttaaaattga ttgcatgcac
    10681 ggcatttcca tttcaaaaca caaaactcaa tcgcccattg ccgccagaag ctcggcctga
    10741 tgctcggcaa tcagggcatt ggtgatttct tccaagtcgc cgtccatcac aaaatccagc
    10801 ttgtgcaggg taaggttgat gcggtggtcg gttacgcggc cttggggata gttgtaggtg
    10861 cggatgcgtt cgctgcggtc gccgctgccg atgagggatt tgcgctcggc ggcttctttg
    10921 gcttgggctt cgcgtttttg cgcgtcgttc aggcgggcgg cgaggacttt cattgcctgc
    10981 gctttgttgg catgttggct gcggccgtct tggcattcga ccaccatgcc ggtgggcagg
    11041 tgggtgatgc ggacggcgga gtcggttttg ttgatgtgct gaccgcccgc gccggatgcg
    11101 cggaaggtgt cgatgcgcag gtcggctggg ttcagttcga tgtcttccag ttcgtccgct
    11161 tcgggcatga cggcaacggt gcaggcggag gtgtggatgc ggccttggct ttcggtggcg
    11221 gggacgcgct gcacgcggtg tccgcccgat tcaaatttca aacggctgta cgccccgagt
    11281 ccgacaatac gggcgatgac ttctttatag ccgcccaatt cgctttcgtt ggcggacacg
    11341 atttcaacct gccaacggtt gcgctcggcg tagcggctgt acatacgcag caaatcgccg
    11401 gcaaacagcg cggcttcgtc gccgcccgtt ccggcgcgta tttcgatgaa gatgtttttg
    11461 tcgtcgtcgg catctttggg cagcagcagt ttttgcagtt cggtatcgag ttcgccgatt
    11521 ttggctttgg ccgcttcgat ttcttcggcg gcaaagtctt tcatttcggg gtcggacaac
    11581 atttcttcgg catccgccaa gtcgctttgg gcaagccgat agttttggaa cacttcgacg
    11641 acgggggtca gttcggcgtg ttcgcgcgtg agcttgcggt agttgtccat gtcggacgtg
    11701 gcttcgggct gtccgagaag gtgggtaact tcttccagtc ggtcgctgag ttgttgtagt
    11761 ttttctaaga tagacggctt cataattctt ccataacaaa cgccgcctga atgttcagac
    11821 ggcatcaaca ctggattatt ataataggtt ttccggatat tcaaaaagat aatcttagat
    11881 ggataaccta ccgtcccaac agggcatcgg cattgcgctc cgttaccttt gcaatctctt
    11941 ctacacaggt tccgcggatt tccgcagcaa tctttgcaat acccggaata ttggcaggcg
    12001 tattaatctc ttttttcagc ataaacgggc tatccgtttc caatacgaaa tccccgtcgt
    12061 tcaaggcttt aagcgtatcg cgcactttac gcgcgttcgg attgagcagc agcgaaccga
    12121 tgccgatttt gaaacccagt ttcgtcaaca cacgcgcttc ttccgcgctg ccggagaagg
    12181 cgtgaacgat gccgccttgg gcaaagcctg tctgtttgac ggcggcggcg atgtcggcgg
    12241 tggctttgag attatggata atcacgcggc ggcgcagggt ttgcgcaatt tcaagctggc
    12301 ggacgaaaac ttgaatttgc cgttcgcgct gctgcgacgt ttgggtttta tcgtaaaaat
    12361 ccaagccgat ttcgccgacc catgcctgcg gataatgtgc caacatcgtt tccaggcgga
    12421 cgaaatcccg ctcggcaatg ccgtctgaaa accaaggatg aatgcccagt gcaatacgga
    12481 tttgaccgtg ttcggacggc atttccgcca aatccgccac gtcctgccaa tcctgcgggc
    12541 gcgtcgcggg aacgataaac cgcttcaccc caactttccg cgctgcggtc aggatgtgcg
    12601 gcaggttttc gcgcagggcg ggatcagcga gatggcagtg ggtgtcggtg aagttcattt
    12661 cgatttcaca ctaactttag tcttaccaat tctttgtaaa catcttcctt accccagcct
    12721 tgcgatacgg cgagggtcat cagcgcggtg gcggtttcga ggttgcattt gccgccgttg
    12781 atgatgcccg agttgcggaa cgcgttgcct tgcgcgtaaa cggcggcggt tttgccttgt
    12841 cggacttggc tgatgttgag cagcagtttg ccctgccgcg cgaagtctcg gacggcgcgg
    12901 ataaaacctt cgtctgcggg cgtgttgccg tgtccgtagc tttgcaggat aagagcttgg
    12961 gcgggaagct gtccgagtcc gtccgcaagt tcttggacgg caaagccggg gataagcgtg
    13021 cggacagcga tttttgcctg cgggtcggga taacggattt tgaggccgtc tgaaacggct
    13081 gctgcgtctt gggacgggag gcgaagattg tgccaacccc gggtttcgtc ccattcggca
    13141 agcgtgccga aatgcggatt gtcgaagcct gcagcagttt cggtgctgac tttgctgctg
    13201 ccgacggcgg gatacagttt gccgtcaaac gcgatgacgg tttgtttgag cttgaggctg
    13261 aaggcggcaa cggcggtgga gaggttgcgc ggggcatcgc tgttttcggc ggcgtaaggc
    13321 cattgggaac cagtcaggac aatcggtttg cccaaacctt gcagagcgag cgcgaggaga
    13381 ttggcggtgt acgccatgct gtccgtgccg tgcagtatca ggatgccgtc gcatgaaggg
    13441 agtttgtcgg caatgatgtc cagccaatcg cgccagtgtt gcagcgtaac ggaggaggaa
    13501 tcaatcaagg gattgcagac gtgccactcg aaatcgaggc cgtctgaaaa gggggaaagg
    13561 gcttggctaa ccagtgcggt atcggggcgc aggccttcgc tgctttgggt catgcctatg
    13621 gtgccgcctg tgtagaggac gaagattttt tgtttcatgg acatcatcgg gtcgtctgaa
    13681 aataataata cggcttattt aactatattt cggacagact ggcaatttgg cggcgcggac
    13741 ggttttcaga cggccttcaa atgaaaaagc acccgagggc tgtcgatatt tgattttcca
    13801 agtagatttt tattcacgaa ataggagagc cgcaacaagc ttaaatccct tgtgaggttc
    13861 ccaacacgga agataccgct ttgtggatta aaaaatacgg aaactattga atatcgacaa
    13921 cctatttagg tgcttgattt tattgtttgc tttgcgcggc ttttttggct gccttggcgg
    13981 ctttgcgttg cgcccgcttt tctttcaatt tgctgcggta aaactggata cgttggcgtt
    14041 ttttccacca aatccaagcg acaacggtcg cacctatacc caagataaca aaaatacccg
    14101 attgcaggct gtgcattttc gccatcagcc aatcgatgtt gtgcgcaccg tattcgccca
    14161 gataaatcca aatagggacg gaaatcagtg cggccagtcc atccataatg ataaaacgca
    14221 agtatgaaac cttgcggctg ataccggctg taacaaatac ggccgttctc aaaccgggca
    14281 ggaaacgggc gacaaataag acccagttac cgtatttgtc gaatttttcc tgaacctgct
    14341 cataacgttt cggcgtcatg atgcgcgcaa taggtttgaa ccttaggatt ttctgccccc
    14401 aaattcgtcc ggcggcgaac atgatgccgt ccccgaccaa tacgccgagc ataccgactg
    14461 caaacataat atgcggattg gtataaccca tacccgaaat cacgccgcct gttaccaagg
    14521 tcaaatcctc gggaatcggc acgccgaaac cgcagatgac caatacaaaa aaaacagccg
    14581 cataaccgta ttcgacaaaa aaggcttcta aaaaagcaaa catggcggat attccattgt
    14641 cggagataaa aagtcagaac aaaccgaaac attttctaca tgaagcaggc attctatcaa
    14701 agattatgcc gtctgaaagc ggaaaaaagg cagattcgcg catcctccgc atgttcagac
    14761 ggcatttaaa cagaaaaccg gccggttcta aaaacggttt tgcctgattt tgcctaaatg
    14821 ccgccgatgg cggcggcaat gcgttccgcc ccttcgcgcg cccaatccgc ctgccgcgcc
    14881 tccaccatca cgcgcacgac gggttcggtt cccgaagcgc gcaacacgac acgccctttg
    14941 ccttcgagtt ctttttccac ttccgccaac acgtctttcg aagcttcctg ccattgctga
    15001 cctttttgga tgcgcacgtt aatcatcgtt tgcggatacg gctgccaatc ggcgcaaacg
    15061 gtggcgaggt cttggttcag cgtttgcagt gccgccaaaa cttgcagcgc ggaaataatg
    15121 ccgtcgccgg tgttgtgttt gtccatacac aaaatatggc cgctggcttc gccgccgatg
    15181 agccagccgc gttggttcag ctgttccaac acatagcggt cgccgacttt ggcgcggcag
    15241 aaatccacgc cctgctcttt cagggcgatt tccatcgcca tattggtcat gaccgtgccg
    15301 accacgccgc cgatgttgat accttctcgg gcgcgggctt tggcaatgac gtaaatcagg
    15361 ctgtcgccgt cgtaaacctg cccgttttta tcgaccatca tcaggcggtc gccgtcgccg
    15421 tctaaggcga tgccgtagtc ggcttcatgc tgtaaaacgg cggcctggag tgtcttggta
    15481 taagtcgcac cgcatttttc gttgatgttg tagccgttgg gttcgttgcc gatgctgacg
    15541 acctgtgcgc ccagttcgtg aaacaccttg ggggcgacac cgtaccccgc gccgttggcg
    15601 gtatcgacaa ccaacttcaa accccgaagg tcggaatggc tgggaaaggt ggatttgcaa
    15661 aattcgatat agcggtcgtc cgcaccgctg atgcggcgtg cgcgaccgag acgggcggac
    15721 ggttgggttt tcatttcgcc gtcgattttg gcttcgattt ccaactcgac ttcatcggaa
    15781 agtttcacgc cgccttcggc gaagaatttg atgccgttgt cggaataggc gttgtgcgac
    15841 gcggaaatca tcacgccggc ggacaggcgc aacgcgcggg tcagataagc cacgccgggc
    15901 gtgggcagcg gtccggtctg taccacattc acacccgccg ccgtaaaacc ggccaccaaa
    15961 gcggcttcca gcatatagcc ggaaatgcgc gtgtctttgc cgatgaggac ggtcggtttc
    16021 tggtcggtgt cgtgctgcac caaaacctgc cccgccgcat agccgagttt caatacgaaa
    16081 tcgggcgtaa tcggaaattg ccccacttcg ccgcgcacgc cgtccgtgcc gaaatatttt
    16141 tttgccatgt gttgctccga gaatgtgaac cgttgtccga gattatacag tcagtttgtg
    16201 ccttgctgtc tgcaccgttg atgccgtctg aaaccgcccc gtccttttca gacggcatga
    16261 agtatgtgaa ccgctgttta cagattgatg cccaacgctt cccacacctt caacgcatcc
    16321 gctgtcgcct tcacatcatg cacccgcacg atttgcgcgc cgcgcgctac ggaagccaac
    16381 gctgccgcca cgctgccgtg tacgcgttcc gccgcatttg cctcgccggt cagctcgcct
    16441 atcgtgcttt tgcgcgatac gccgatgagc agcggaaaac ctgtttccgc catcaattcg
    16501 ggcaaatgcc gcatcagcgc gatattgtgt tgcaagggtt tgccgaagcc ggagccgaag
    16561 ccggggtcga gtatgatgcg ttgcggtgcg atgcctgccg cgatacattc cgctgagcgc
    16621 gctttcaaat accgcgctac ttcaccgaca acatcttgat atttcggatt aatctgcatg
    16681 gttttgggca aaccctgcat gtgcatcagg caaatgcccg tgtccgcctg acgcgccagc
    16741 aattcgaccg cgccctcgtc attcaacgcc gccacatcat taataatatc gatgccgccg
    16801 agtgccaacg ctttttccat aatcaccgtg cggcgcgtgt ccaaactgat gggaacgccc
    16861 caccccgcca cttccgccaa aacaggctca acccgcgccc attcttcttc aggcgaaaca
    16921 taatccgcac ccgaccgcgt cgattcgccg ccgatgtcga gaatgtctgc gccttctttt
    16981 agaagctgtt cggcatgtgc caaggctgtt tgggcgtttt gcgaatacac gccgccgtcg
    17041 gaaaaagaat cgggtgtgag attcacgatg cccatgattt tcggtttgtc caaaccgatt
    17101 tcaaaccgtc ctgcctgcca aacgtgtcgt gccatctgaa ctcctcccaa aataaaaaac
    17161 agattatatg ccgtctgaaa ccgtcttgtg cgcttcagac ggcaccgcta ttcgggcggc
    17221 agacggcatg ttgtccgaat gtctgctccg cctttgaatc tgccggtatg cctgctatcc
    17281 gcccgacttt tcaaaacagg ttccgacgat tccgcacgcg cctgccgcct ttgccaagcc
    17341 gtacaggatt tcctgcggca tatcgcggtt ccataatccc gtaatattcg caatcacggg
    17401 cagatggctg atttggcgga ctttcacgat ggattcgaca tccaaacggt agggatggcc
    17461 tttggtatgg ttcaatacgc ccgactcgcc cagaatcagg tggcggttgc cgcgcgagac
    17521 gacatattct gcggcattca accaatcttc ggcagaatgg tgcttgtctt tacacagcac
    17581 caagggaatg ttcaggtttc cggcttcatt caatacggca agatcggaca tcagcccgcc
    17641 gcccaaatac aggatgtccg cccccgcatt caaagccgct tcgacatggc ggacgttgcg
    17701 gacgcgcacc aatacgggtt tccctgcatc atgcgccgat gcggtctgtt ccgccaaccg
    17761 tctgcaccgt ccccgccctt catccgcact tgaagtgtcg tataagtttg ccgaagtgaa
    17821 aaacggatcc agaaacactg catccgcatt gcgccatact gacggttctg cggcgatacg
    17881 gacggtttcc ccgccgccga aagccacgcc tttggcggca acgcggctgt cttccgcccg
    17941 attttcccga ctgacggttt tccatgtatc caaaatgcgg acggctttct cgacctccgg
    18001 cagcgtctgc acctccctga cgctcaaaac cctatcgtcg ccgattgcgc cgatgacagt
    18061 acgctcgtcg ccgtgagaaa tgtgttctcg cagacctctg ctgcggataa aggcgacaac
    18121 gccggcaatg tccgcttcgg cggcacgcct gctcatgaca ataatcatat ttcctcctga
    18181 cacaagaaac ggcctaccca aaataggatt tttgcaagcc gtgttatact gtggcgtgtt
    18241 ttacagattg ttcgggctat ggatttatta tcggttttcc acaaataccg tctgaaatat
    18301 gcggtggccg tgctgacgat actgcttttg gcggcagtcg ggctgcacgc ttccgtatat
    18361 cgcaccttca cgcctgaaaa catccgcagc cgcctacaac aaagcattgc acacacacac
    18421 cggaaaatct cgtttgatgc ggacattcag cgcaggctcc tgccccggcc gaccgtcatc
    18481 ctgaaaaacc tgaccattac cgaacccggc ggcgaccaga ctgccgtttc cgtccaagaa
    18541 accaaaatcg gattgagctg gaaaaacctg tggtcggatc agatacagat tgaaaaatgg
    18601 gtggtttcga gtgcggaact tgccctgacg cgcgacggga aaggtgtttg gaacatccaa
    18661 gacctgatcg acagccaaaa acgccaagcc tcagtcaacc gcattatcgt cgaaaacagc
    18721 accgtccgcc tcaatttcct gcaggaacag cttatcctga aggaaatcaa cctcaacctg
    18781 caatcccccg attcgtcggg gcagccgttt gaaagttcgg gcatactggt ttggggaaag
    18841 ctgtccgtcc cgtggaaaag cagggggctg ttcctttcag acggcatcgg cacgcccaaa
    18901 atctcaccgt tccattttga agcttccact tcgctggacg gacacggcat caccatttcc
    18961 accaccggca cacccgccat ccgtttcaac gccggcggag cggatgccgc cggcctcggc
    19021 ctgcgtgcag acacttcctt ccgcaacctc cacctgaccg cgcaaatccc cgcgctggca
    19081 ctcaggaaca acagcattaa aattgaaacc gtcaacggcg catttaccgc cggcggcgaa
    19141 tatgcccaat gggacggttc gttcaaactc gacaaagcca acctgcactc cggcatcgcc
    19201 aacatcggca acgccgaaat ctccggcagc ttcaaaacac cgcgccacca aaccaacttc
    19261 tccctcaatt cgccgctcgt atggacggaa aacaaagggc tggacgcgcc gcgcctgtat
    19321 gtatcgaccc ttcaggatac cgtcaaccgc ctgccgcaac cccgtttcat cagccggctc
    19381 gacggttcgc tgtccgtacc gaatctgcaa aattggaatg ccgaattaaa cggcacattc
    19441 gaccgccaaa ccgttgccgc gaaattcaga tacacacatg aagacgcacc gcatctggaa
    19501 gccgccgtcg cactgcaaaa attgaacctg accccctatc ttgacgacgt gcggcaacaa
    19561 aacggcaaaa tatttcccga caccctcgcc aagctgtccg gcgacatcga ggcgcacctg
    19621 aaaatcggaa aagtccaact tcccggcctg caactggacg atatggaaac ctacctccac
    19681 gccgacaaag gccatatcgc gctcagccgt ttcaagtcag ggctttacgg cggccatacc
    19741 gaaggcggca tcagcatcgc caacacccgt cccgccactt accgcctgca acagaatgca
    19801 agcaacatcc aaatccaacc gctgctgcaa gacctgttcg gcttccacag cttcagcggc
    19861 aacggcgatg cggtcatcga cctgaccgcg ggcggcgaaa cccgaaaaga gcttatccgc
    19921 tcgcttcaag gcagcctgtc gctgaacatt tccaacggtt cgtggcacgg tatcgacatg
    19981 gacaatatcc tgaaaaacgg catttcgggc aaaactgccg acaatgccgc acccagcaca
    20041 cccttccacc gattcacgct caacagcgaa atttcagacg gcatcagccg ccacatcgat
    20101 accgaactct tctccgacag cctctatgtt accagcaacg gctataccaa tctggatacg
    20161 caggaattgt ctgaagatgt ccttatccgc aacgccgtcc atccgaaaaa caaaccgatt
    20221 cccctgaaaa tcaccggtac ggtggacaaa ccgtccatta ccgtcgatta cggcaggctg
    20281 accggcggca tcaattcgcg caaagagaaa cagaaaatcc tcgaagacac cctgctggaa
    20341 caatggcagt ggctcaaacc taaagaaccg taaacatcct gcgtacacaa atgccgtctg
    20401 aaacgccccc gcgcttcaga cggcagaccg taaaacctac aaccccaatt cctcccaaat
    20461 cccatcaatc ttagccgtaa ccgcagggtc ttttttgatg acgcgtcccc attcgcggtc
    20521 ggtttctccc ggccatttgt tggtcgcatc caaacccatt ttgccgccga gtccgctgac
    20581 ggggctggcg aagtcgagat aatcgatggg cgtgttttct accaaaacag tgtcgcgcac
    20641 ggggtccatg cgcgtggtta ccgcccagat gacttctttc cagtcgcgta cgttcacatc
    20701 gtcattcacc acgatgatga atttggtgta cataaactgg cgcaggaacg accagcagcc
    20761 catcatcacg cgcttggcgt gtccggcgta ctgttttttc atgctcacca ccgccatgcg
    20821 gtaggagcag ccttcgggcg gcaggtaaaa atcggtgatt tcggggaact gcttttgcaa
    20881 gagcggtacg aacacttcgt tcaatgccac gcccaaaaca gcgggttcat cgggcggttt
    20941 gcctgtgtag gtcgaatggt aaatcgggtt ttcgcgcatg gtgatgcgtt cgaccgtgaa
    21001 cacagggaaa tgatcctgct cgttgtaata gcccgtgtgg tcgccgtatg ggccttccag
    21061 cgcggtttcg ttcggatgga tgacgccttc caacacgatt tcggcacggg caggcacttg
    21121 caaatcgttg ccgatacatt tcaccagctc cgtccgcgaa ccgcgcagca gtccggcaaa
    21181 ctggtattcg ctcaaggtat cgggaacggg cgttaccgcg cccaaaatgg tggcagggtc
    21241 gcagccgagc acgacggcga cgggatacgg cgtatcggga ttgagtttgc ggaattcctg
    21301 ataatccaac gcgccgccgc gatgcgacag ccagcgcata atcagtttgt ttttgccgat
    21361 gagttgttgg cggtaaatgc cgagattttg gcgttttttg tgcggcccgc gcgtgacggt
    21421 caagccccat gttaccagcg gcgcaacgtc ttccggccag caatgctgaa tcggaagttg
    21481 atacaaatca acgtcttcgc cttcccacac gatttcctga cacggcgcgt ttttcaccac
    21541 gttcggcgcc atgctccaaa tgtctttcag cagcggcagt ttggaaaacg catctttgat
    21601 gcctttgggc ggttcgggtt ctttcaaata cgccagcgtc tgcccaattt cacgcagctt
    21661 ggacacgctg tccgcgccca tgcccatcgc cacacgttcg ggcgtgccga acaggtttgc
    21721 caacacggga taaccgtagc gcgtaccgtc gggcttaatc gggttttcaa acagcaacgc
    21781 cggcccttcg gcacgcagca cgcggtcggc gatttcggtc atttccaaat acggggaaat
    21841 ggggtgtgcg acgcgcttga gtttgccctg ctgctcgagc atggcgatga agtcgcgcag
    21901 gtctttgtat ttcatattca tcctttttgt ccttttatcc tgaacaatcc gattcggata
    21961 ccgcccctat ccttgcctgt gcctcggcat attctatgcc gtgataaaag tcgcgtacca
    22021 gcggatgttc gccgccttga tggagttgca acaaaggacg ttgaccatcg ggttgggtaa
    22081 cgacattgca gtgcagaccg aaggtgtcgg tttcataagg gggcagctgg ttgcagatca
    22141 tgccgaaata aacagcgttt tcaaggttgt cgtaaaagcg gctttgatag tcgttaaaac
    22201 tcttttcgct gacggatacc cacacgccat attccagcgt ttcctcatgc ccgataatcg
    22261 gaatcggcag caccgcgcgg ataaagcggt cggtttggtc gggatagcgg atgatgcaga
    22321 aatcagaatc gcattctgct tgataagcaa tgcgttcttc ttcactgagt tgattatagg
    22381 gatcgggtgc ggtaaagccg attgcgggca tttcttcgtg gttttcgccg caggaagtgc
    22441 aagtgtacat aaggtttcag actttcaaaa cgagtttgcg gtaaagccat tcgccggcaa
    22501 acagcatccc catcaataca taggcaatca cgccggtata aaccgcccac caatcatatc
    22561 gccccaaccg tgccaacaaa gcggcaagcg tcccgttgat tataaaaaat acgcaccaaa
    22621 cctgcgttac ccggcgggta tagcgcacgg ctttttcagg caggtcgggc tgttgcagcc
    22681 gcgcaagttt ttcaatcacc gtctgcccgg caaacaggct gccgccgaac accgccaaca
    22741 tcatcagatt gacgaggacg ggataccaat acatcgaatc atgccgcccg aacaccaata
    22801 ctgcggcaaa aaacagtgta ataaacaaag ccgcataacg ctgttggggc agtttggcag
    22861 tcagggcgcg cagcagccac aacccgcaca tcgccgccgc cagccaaaca aaccagcccg
    22921 cctccctgcc gtatatagtg gattaacaaa aaccagtaca gcgttgcctc gccttgccgt
    22981 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac cacaaagcgg
    23041 gataggcaat gcttaatacg gtcagaaaaa tatgtccgaa aaaaccgggt ttcattttga
    23101 atccgcacaa atgttttcag acggcatccg ataaaaacat gccgtctgaa aaataattag
    23161 aaatacccga ttagcccgcc tgaatcttca ataccgcctg taccacgtcg ttgacggtgc
    23221 ggacattgcg gaaatcttcg gcctgcagct tgcggccggt ttcgcgcttg atgcggtcaa
    23281 tcaggtcgat ggcatcgatg ctgtcgattt ccaaatcttc gtaaagattg gtatcaggcg
    23341 taatccgttc cggttcgatt tcaaacaact cggtcagggt atcgcgcaac aaccggtaga
    23401 tttcttgttc ggtcatatgt ttcatcctta tgcttggcgg cttttgacaa aggcggcgag
    23461 tgttttgaca ttggcaaaat gttcgcgcaa gttctcctgc tcgccgtcca atcggaaacc
    23521 gaaatgtttt tgcaccgcca agcccaattc cagcgcatcg acggaatcca gtcccagccc
    23581 tccgtcgccg aacagcgcgt cttcgctgcc gatgtcggcg gcggttatat cctccaaagc
    23641 caaactgtcg ataatcagtt gtttgatttg gttttccaag tcgttcatat ggttttcctt
    23701 gtaaaatagt cctgcagata ttcattcagc cgccgcgccg caatcggcaa cggtttctcg
    23761 gcgagccagt cttgggggag gatgtcgtct ccgacggtaa tttcataccg tatccttttc
    23821 ggggggatgc ggtaccacgg ctgccccttt ttaaaattgg gcggattcat tttgatacat
    23881 acgggcgtaa tcacttcggc ataccgcagt cccaaagaaa ccgcgccccg gtgcattttt
    23941 acccgtccgt cccaccccgt cctcgttcct tcggggaaca ccagcaggct ctgcccgctg
    24001 tcaaaaaccg cctttaccgt ttccagcatt gcttccgact cttcgttcgg aatatagccc
    24061 gcacctttaa tctggctgct cattgccgga ttgtgctgca aatctttttt cacgatacag
    24121 ttcatttcgg gcacatggcc gacaagcagc accacatcca gcaaagacgg atggtttgcc
    24181 aaaatcaact gtcccgggcg gttgagtttt tcaacaccct tgaacgatac ctccaacacg
    24241 cccgaccatt tcagataagc aacgaacaaa cgccaagatg ttccgataat ccggcgcgcc
    24301 gccaattggc gggcgacaga ccctgaagtg ccgttcaaag tataaggcaa caaaaccaat
    24361 ttcatcataa tgccgccgac accgaaaatc acaaatccta accaagtcgc aaaaaaacgg
    24421 cggcaataat ccaatttatc cattccgctg ccacagccat tcacgattgc gatatacccg
    24481 gcggcattct cgactgccgt tcagcagaaa gcgcacccat tccaaaccgc tccaatatgc
    24541 ctcgggcagc ataccggctt cagacggcat atcgtccgaa gcagacaaag tcaggctgta
    24601 acgcgtccct ttggtcagaa ccatcgccaa agcataagca aacggcgcgc gagtcgccga
    24661 tacggcatat ccttccggca gcggatcgtc cgccgccaaa accaaaaccg acccgcatcc
    24721 ctcttccaac agtgatgccg cttccgccaa tgccgtttcc acaccgtcgg cacacacggc
    24781 caatgccgtc tgctcgctca tatcctgacg caatatcgac cattgccccg ccgttgcatt
    24841 gtgcaccgac aaaccgaacg aagtcggcga cacggtatgc gatttcaaca gttccaacca
    24901 caaatcgaaa ctgcgtgcca tttccccgtc gtgcgaggca taaactaccg gactgccggg
    24961 atgggcggag gcaatgtccc aagccgcgtc gcataccaaa cgcgccgcct tactcaaacg
    25021 gcggcgctgc atagcgggca ggaacggcaa ttccggcctg acatcgggca aaccgtcggc
    25081 aaaatccgga cattccgccc attttgccca ctgggccata tcgcgcattt tgctgcccga
    25141 aacccgccag gcggcgatgt cgaagtggaa ccggcaaata atcgacggca tagtttcttt
    25201 caaaaattta cactgtgccg cattctaacc aaagcctatc cccctgacaa tgccgaaatt
    25261 caaacgcatt tctgccccct ttctccgaca acgccgcccc tcggaaaacc gccagaatta
    25321 gcctgaattt acatttatca ttataatgcc cgtatttgcc agcctgccgc cgcaatatat
    25381 ggacacactg ccagaatgcc cgattaccaa caccgcctcc ctgctgccgc acagcgggcg
    25441 tatggttctg atagaccgca ttacccgata cggcgatgat tttgtcgaag caggggcaca
    25501 ggtaagcccc aatcacatcc ttttacttga cgacaaactg ccctacacgg catttatcga
    25561 actgatggca caggctgtcg gcgcgtatgc cggtatccaa gcccgaaaaa acgcacggtc
    25621 ggtccggctc ggcttcctgc tcggcacgcg caaacttgaa atcttcgccc aatccgtccc
    25681 aatcggcacg catctgctgg caacggcgca tatgtctatt caggatgccg ggggtatggg
    25741 cgtgtttgac tgcgaactgc gttggacaga cgcgccggaa acttcgtccg aaacactccc
    25801 ttcagacggc attttggcgc gcgcctcact caacgtgtac agccccgaac accctgccgg
    25861 aacaaccgat gccgtctgaa caggcacaca catacggaga acaacgatga ccgaaactgt
    25921 cctgattacc ggctccaaca ggggcatagg caaagccgtc gcattcggtt tggcggaaga
    25981 cggctttgat atcgctgtcc actgccgcag tcgccgcgac gaagccgaag ccgtggcgga
    26041 agaaatccgc gctttgggca gaaatgcgcg cgtgttgcag tttgacgtgt ccgaccgcga
    26101 agcctgccgc gagattctga ccgccgacat cgaagcaaac ggcgcgtatt acggcgtggt
    26161 gttgaacgcc ggactgacgc gcgacaatac cttccccgcg ttttcagatg acgattggga
    26221 tgtggtgctg cggactaatt tggacggttt ttacaatgta ttgcatccgc tggttatgcc
    26281 gatgatacgc cgccgcaaag ccggacggat tgtgtgtatg gcatcagtgt ccggcctgac
    26341 gggcaaccgc gggcaggtca attacagcgc gtcaaaagca ggcattatcg gcgcggcaaa
    26401 agccttggcg gtcgaactgg cgaaacgcaa aatcaccgtc aactgtgtcg cgccgggtct
    26461 catcgatacc gatattatcg atgagaacgt acctgtcgaa gaaatcttaa aggctgtccc
    26521 cgcagcgctt atggggctgc cggaagaagt ggcgcacgcg gtgcgtttcc tgatggatga
    26581 aaaagcggcg tacatcacgc gccaggtgat tgcggtgaac ggaggtttgt gttgaatacc
    26641 agaagggtcg cagtaacagg cataggcggc attaccgcct tcggccggga ttggcaaagc
    26701 atacaggcag cattcaaagc cgaaaaaaac gccgtcaaat atatggattg gcacgaacgt
    26761 ttccccgaat tggaagcgca actgggtgcg ccgattgaaa attacgcgcc gccgaaacat
    26821 tggacgcgca agcagctcag aagtatgggg cgcgtgtcgt acctgtgcgt cgatgcggcg
    26881 gagcaggcgc tggcggatgc cggtttgctc ggggacgaaa gcattaccga cggacggatg
    26941 ggcgttgcct gcggctcttc cagcggcagc accaaagaca tcggcgatgt gggcgaattg
    27001 ttgctgaccg gcacgtcgcg caacttcagc gccaacacct atgtgcgtat gatgccgcac
    27061 accaccgccg ccaatatcgg catctttttc gggctgaaag ggcgcatcat cccgacatcg
    27121 agcgcgtgtt cgtccggcag ccaaggcata ggttatgcct acgaagccat caaatacggt
    27181 ctgaccgata tgatgctggc gggcggaggc gaagaatttt tcccgtccga agtgtatgtt
    27241 ttcgactcgc tttatgccgc cagccgccgc aacggcgaac cggaaaaaac cccgcgccca
    27301 tacgacgcga accgcgacgg gctggtcatc ggcgaaggcg cggggatttt cgtgctggaa
    27361 gaattggaac acgccaaacg gcgcggtgcg ataatttacg ccgaactcgt cggctacgga
    27421 gccaacagcg atgcctacca tatttccacg ccccgccccg acgcgcaagg cgcaatcctt
    27481 gcctttcaga cggcattgca acacgcaaac cttgcacccg aagacatcgg ctggattaat
    27541 ctgcacggca ccgggacgca ccacaacgac aatatggaaa gccgcgccgt tgcagcggtt
    27601 ttcggcaaca atacgccctg cacgtccacc aagccgcaaa ccggacacac gctgggcgcg
    27661 gcggacgcaa tcgaagccgc gttcgcgtgg ggcattgccg accggcaaag caatcccgaa
    27721 ggaaaacttc cgccccggct ttgggacggg cagaacgacc ccaacctgcc cgccatcaac
    27781 ctgaccggca gcggcagccg ctgggaaacc gaaaaacgca ttaccgccag ctcgtcgttt
    27841 gccttcggag gaagcaactg cgtcttaatc atcggatgaa ataagtttgt caatcccacc
    27901 gctatgctat acaatacgcg cctactcttg acgggtctgt agctcagggg ttagagcagg
    27961 ggactcataa tcccttggtc gtgggttcga accccaccgg acccaccaat tcccaagccc
    28021 ggacgtatgt ttgggctttt ttgccgccct gtgaaaccaa aatgctttga gaaaccttga
    28081 taatgaaaaa agtcagcgta ttgattgttg ccaaaaacga agcaaaccac attcaggaat
    28141 gtattgaaag ttgccgtttc gataaagaag ttatcgttat cgacgactac agcaccgaca
    28201 atactgccga aattgccgag ggtttgggcg caaaagtctt cagacggcat ttgaatgggg
    28261 atttcggagc gcaaaaaaca tttgccatcg aacaggcagg cggagaatgg gttttcctga
    28321 ttgatgcaga cgaacgctgc acgccggaac tatctgatga aatctcaaaa attgtccaaa
    28381 ccggcgatta tgccgcctat tttgtcgaac gccgcaacct tttccccaac catcccgcca
    28441 cacacggcgc gatgcgtccc gacagcgtat gccgtctgat gccgaaaaaa gacagttcgg
    28501 tgcaaggcaa agtacacgaa accgtacaaa ccccctaccc caaacgccgt ctgaagcatt
    28561 ttatgtacca ttacacgtac gacaactggg aacaatattt caacaagttc aacaaatata
    28621 cttccatttc agccgaaaaa taccgagagc agggaaagcc cgtgcgtttc gttagggaca
    28681 ttatcctccg cccgatttgg gggtttttca aaatttatat cctgaacaaa gggtttcttg
    28741 atggaaaaat gggttggatt atgtccgtca accacagcta ttacacgatg attaaatatg
    28801 tcaaactata ttatctgtac aaatccggcg gaaaatttta aatggaaaaa gaattcagga
    28861 tattaaatat cgtatcggcc aagatttggg gtggaggcga acaatatgtc tatgatgttt
    28921 caaaagcatt ggggcttcgg ggctgcacaa tgtttaccgc cgtcaataaa aataatgaat
    28981 tgatgcacag gcgattttcc gaagtttctt ccgttttcac aacgcgcctt cacacgctca
    29041 acgggctgtt ttcgctctac gcacttaccc gctttatccg gaaaaaccgc atttcccacc
    29101 tgatgataca caccggcaaa attgccgcct tatccatact tttgaaaaaa ctgaccgggg
    29161 tgcgcctgat atttgtcaaa cataatgtcg tcgccaacaa aaccgatttt taccaccgcc
    29221 tgatacagaa aaacacagac cgctttattt gcgtttcccg tctggtttac gatgtgcaaa
    29281 ccgccgacaa tccctttaaa gaaaaatacc ggattgttca taacggtatc gataccggcc
    29341 gtttccctcc ctctcaagaa aaacccgaca gccgtttttt taccgtcgcc tacgccggca
    29401 ggatcagtcc agaaaaagga ttggaaaacc tgattgaagc ctgtgtgata ctgcatcgga
    29461 aatatcctca aatcaggctc aaattggcag gggacggaca tccggattat atgtgccgcc
    29521 tgaagcggga cgtatctgct tcaggagcag aaccatttgt ttcttttgaa gggtttaccg
    29581 aaaaacttgc ttcgttttac cgccaaagcg atgtcgtggt tttgcccagc ctcgtcccgg
    29641 aggcattcgg tttgtcatta tgcgaggcga tgtactgccg aacggcggtg atttccaata
    29701 ctttgggggc gcaaaaggaa attgtcgaac atcatcaatc ggggattctg ctggacaggc
    29761 tgacacctga atctttggcg gacgaaatcg aacgcctcgt cttgaaccct gaaacgaaaa
    29821 acgcactggc aacggcagga catcaatgcg tcgccaaccg ttttaccatc aaccataccg
    29881 ccgacaaatt attggatgca atataaactg ctttcagacg gcatatgccg tctgaaagcc
    29941 tttgatgcaa caaaccgcca aattatattc gttcattgga aagaaacacc ccgaatccat
    30001 ccttcaaaat aagaaaatcc caatatcccc cgatattacg cagcctattg gcaaagtttt
    30061 gcagcgtctt ccccggcttg tgctgccgca tcaagtgctt tgttacaatg tatagtaga
//
LOCUS       DAWISC010000006        27738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWISC010000006 DAWISC010000000
VERSION     DAWISC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.006"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            182..538
                     /locus_tag="WIW87_000183"
     CDS             182..538
                     /locus_tag="WIW87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ5173404.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="WIW87_000184"
     CDS             529..1011
                     /locus_tag="WIW87_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ5173405.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="WIW87_000185"
     CDS             1024..1617
                     /locus_tag="WIW87_000185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ5173406.1"
                     /translation="MASIQDLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="WIW87_000186"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="WIW87_000186"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ5173407.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLVGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="WIW87_000187"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="WIW87_000187"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ5173408.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="WIW87_000188"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="WIW87_000188"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ5173409.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="WIW87_000189"
     CDS             5027..5464
                     /locus_tag="WIW87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173410.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5708..5989
                     /locus_tag="WIW87_000190"
     CDS             5708..5989
                     /locus_tag="WIW87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5173411.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNTE"
     gene            6301..8562
                     /locus_tag="WIW87_000191"
     CDS             6301..8562
                     /locus_tag="WIW87_000191"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ5173412.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGKQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTSAI
                     LKDALDAESLPSKLDNRSTWVGDGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTVVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8565..9641
                     /gene="nuoH"
                     /locus_tag="WIW87_000192"
     CDS             8565..9641
                     /gene="nuoH"
                     /locus_tag="WIW87_000192"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ5173413.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            9722..10201
                     /gene="nuoI"
                     /locus_tag="WIW87_000193"
     CDS             9722..10201
                     /gene="nuoI"
                     /locus_tag="WIW87_000193"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ5173414.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            10232..10939
                     /locus_tag="WIW87_000194"
     CDS             10232..10939
                     /locus_tag="WIW87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173415.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10975..11646
                     /locus_tag="WIW87_000195"
     CDS             10975..11646
                     /locus_tag="WIW87_000195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ5173416.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDDLKPK
                     EEGKA"
     gene            11643..11948
                     /gene="nuoK"
                     /locus_tag="WIW87_000196"
     CDS             11643..11948
                     /gene="nuoK"
                     /locus_tag="WIW87_000196"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ5173417.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            11982..12557
                     /locus_tag="WIW87_000197"
     CDS             11982..12557
                     /locus_tag="WIW87_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ5173418.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12681..13022
                     /locus_tag="WIW87_000198"
     CDS             12681..13022
                     /locus_tag="WIW87_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173419.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            13071..15095
                     /gene="nuoL"
                     /locus_tag="WIW87_000199"
     CDS             13071..15095
                     /gene="nuoL"
                     /locus_tag="WIW87_000199"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ5173420.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTIHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15191..16687
                     /locus_tag="WIW87_000200"
     CDS             15191..16687
                     /locus_tag="WIW87_000200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ5173421.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPFARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIEQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAILAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16697..18139
                     /gene="nuoN"
                     /locus_tag="WIW87_000201"
     CDS             16697..18139
                     /gene="nuoN"
                     /locus_tag="WIW87_000201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ5173422.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFTFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMFAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVIYFDVPDHDQPVGSNYAAKFV
                     LTVNAFLLLLWGIMPQTVIDWCAKALENTL"
     gene            18251..18550
                     /locus_tag="WIW87_000202"
     CDS             18251..18550
                     /locus_tag="WIW87_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ5173423.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18659..19555)
                     /locus_tag="WIW87_000203"
     CDS             complement(18659..19555)
                     /locus_tag="WIW87_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ5173424.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19539..19763)
                     /locus_tag="WIW87_000204"
     CDS             complement(19539..19763)
                     /locus_tag="WIW87_000204"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ5173425.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            complement(20010..20933)
                     /gene="rsgA"
                     /locus_tag="WIW87_000205"
     CDS             complement(20010..20933)
                     /gene="rsgA"
                     /locus_tag="WIW87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ5173426.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(20938..22794)
                     /locus_tag="WIW87_000206"
     CDS             complement(20938..22794)
                     /locus_tag="WIW87_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5173427.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22820..22957)
                     /locus_tag="WIW87_000207"
     CDS             complement(22820..22957)
                     /locus_tag="WIW87_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173428.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(22957..23541)
                     /gene="ruvA"
                     /locus_tag="WIW87_000208"
     CDS             complement(22957..23541)
                     /gene="ruvA"
                     /locus_tag="WIW87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ5173429.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(23623..23949)
                     /locus_tag="WIW87_000209"
     CDS             complement(23623..23949)
                     /locus_tag="WIW87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5173430.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24011..24730)
                     /locus_tag="WIW87_000210"
     CDS             complement(24011..24730)
                     /locus_tag="WIW87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ5173431.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24727..24900)
                     /locus_tag="WIW87_000211"
     CDS             complement(24727..24900)
                     /locus_tag="WIW87_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ5173432.1"
                     /translation="MKRGGNTVTQAAFERRFMPSENLSGHIPNRRPHCTAVRNVSGGH
                     DAHSSSTQERNTF"
     gene            complement(24860..25324)
                     /gene="trmL"
                     /locus_tag="WIW87_000212"
     CDS             complement(24860..25324)
                     /gene="trmL"
                     /locus_tag="WIW87_000212"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ5173433.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25389..25886)
                     /locus_tag="WIW87_000213"
     CDS             complement(25389..25886)
                     /locus_tag="WIW87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ5173434.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            25942..26862
                     /gene="bioH"
                     /locus_tag="WIW87_000214"
     CDS             25942..26862
                     /gene="bioH"
                     /locus_tag="WIW87_000214"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ5173435.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFVRPFDIAAAADGIAAQIDAPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPQALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGGLR"
     gene            26859..27647
                     /locus_tag="WIW87_000215"
     CDS             26859..27647
                     /locus_tag="WIW87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ5173436.1"
                     /translation="MNHQDARWQVHRHLAEHTDQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         6136 a         6890 c         7719 g         6993 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc atcggcctcg cggccggcgt
      241 gctgtttatc ctgctcggca cgattttagg cccgaaacgc cactatgccg aaaaagacgc
      301 gccttacgaa tgcggttttg aagcttttga aaacgccagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct tcatcctgtt tgatttggag gtcgcgttta tgctgccgtg
      421 ggcagtcgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 tgttctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgctgaa ctatatgcgt
      601 accggttcgt tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttacga ccttgaccgt ttcggtatta ttttccgtcc gtccccccgt
      721 caggccgacc tgatgattgt ggcgggtacg ctcaccaata aaatggcgcc cgccctgcgc
      781 cgagtgtacg accagctcgc cgagccgcgc tgggtattgt ctatgggctc atgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagatgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accattgcgc gtgacgagta aggagaggac
     1021 gatatggcaa gcattcaaga cttatacgaa accgtcagcc gcgttttggg caatcaggca
     1081 ggcaaagtca tttccgcttt gggcgagatt accgtcgagt gtctgcccga gcactatatt
     1141 tcagtcatga ccgcattgcg tgaccatgaa gagttgcatt tcgagcttct ggttgacttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagcatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagt tgctttccgt taaaaacaat caacgcatcc gcgtgcgcgt ctgggtttca
     1321 gacgacgact tccccgtagt cgaatctgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatc atgttcaaca accatccgga cttgcgccgc
     1441 atcctgaccg attacggctt cgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatt
     1561 gagccgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ctggacggcg aacaaatcgt ccgtgccgac ccgcatatcg gcctcttgca
     1741 ccgaggtacc gaaaaactgg cggaaaccaa aacctatctg caagccctgc cctatatgga
     1801 ccgcttggac tatgtttcca tgatggtcaa tgagcaggcg tattgtttgg cagtagaaaa
     1861 acttgtcggt atcgatgtgc ccatccgcgc ccaatacatc cgcgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcat gccttcgaca tcggcgcgat
     1981 gaccgccatt ctttacgcct tccgcgaccg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgcg cgtatgcacg ccgcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgactttatg cccaaatacg agggcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca ccatgctcga ctttatcgat gccttctgcg aacgcttccc
     2221 caaaaatatc gacacactcg aaaccctcct gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagaatct
     2881 ttaaaacaaa tcgacatcga gttggcaaaa tatcctgccg accaacgccg ctccgcgatt
     2941 atgggcgcat tgcgtattgc ccaaaccgaa aaaggctggc ttgctcccga gaccatcgct
     3001 tttgtcgccg actacatcgg catcacgcct gcacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggcgacg ctcccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtagc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gcggcggcta taccgccctg cgtaaaattc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggtttgcgcg
     3901 ggcgcggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 cgggcgaaaa atatgtggtt tgcaacaccg acgaaggcga accaggtacg tttaaagacc
     4021 gcgacatcat catgttcaat ccgcatgccc tgatcgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaatttgaa ctcttcgccc accacggcta cggcgcatat atttgcggcg
     4261 aggaaaccgc attgctcgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaagccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat actgaaacgt
     4381 tctcctccgt tccattcatt atccgtgacg gtggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggtacg ccgtttgccg aagttctgaa aatggcgggc ggtatgcgcg
     4561 gcggtaaaaa acttaaagcc gtcattcccg gcggttcgtc cgcgccagtt ttgcctgccg
     4621 atatcatgat gcagactaat atggactatg actcgatttc caaagcaggc tcgatgctgg
     4681 gttccggcgc gattatcgtc atggacgaag acgtgtgcat ggtcaaagcc cttgagcgtt
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac cccctgccga gaaggtacgg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggtaaaatg gaagatttgg
     4861 atttgctgga ttccgtcggc aaccaaatgg caggccgcac catctgcgcc ctcgccgatg
     4921 ctgccgtctt ccccgtccgc agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cgggccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggggaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaatgagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tacgcgggaa tgacggcggg agtaggcaga
     5641 tgttttcaga tgaaaacggt tgtaaatgat attaaaaaag ttgttgttta tattgcagga
     5701 aaaatgaata cgaaaccatc cgcttactag acaacctgcc gtatatattt tggcaaacgg
     5761 taaaaatgga acactctata tcggtgttac catgaatttg ccggaaaggg tttggcagca
     5821 caaaaaccat gtcaatattg atggctttac tgcccgatat gatgtgcatg atttagtttg
     5881 gtatcagttt tttgagaata tgcctgaagc agttgccaaa gaaaaaacga tgaaaaaatg
     5941 gcgacgtgaa tggaagatta aactgattga agaacaaaat actgaatgat tggacttgtc
     6001 gggcgtgttg tttgtttagt tttatttctg gaactttaaa aactgtcgtt attccagccc
     6061 cacctacgcg cagacaggct acggcgggaa tcaccgcaaa agttaagaaa ccaatgtttg
     6121 aaaacagtta ccgaaaaccc aagaatggat tcacgcctgt gcgggaatga cggcaaggtg
     6181 gcagtaaacg ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaaaccc
     6241 atttttcaga cgacctccat aaaagattat ttatcaaata cccgtaacta ggaacgaacc
     6301 atgttacaaa tcgaaatcga cggcaaacaa gtatctgtgg agcagggcgc gacggtgatt
     6361 gaagccgcgc acaagctcgg tacttatatt ccgcatttct gttaccacaa aaaactttcc
     6421 atcgccgcca actgccgtat gtgtctggtg aacgtagaaa aagccccaaa acccctgcct
     6481 gcctgtgcca cgccggttac agacggcatg attgtgcgta cgcattcggc aaaagcccga
     6541 gaggcgcagg aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc
     6601 tgcgaccaag gcggcgaatg ccagttgcag gatttggcgg tgggctacgg caaaaccacc
     6661 agccgctaca ccgaagaaaa acgttccgtc gtcggcaaag atatggggtc cttggtttcc
     6721 gccgaggaaa tgagccgctg tatccactgc acccgctgcg ttcgtttcac tgaagaaatc
     6781 gccggtttgc aggaaattgc gatggtgaat cgcggcgaac actccgaaat catgcccttt
     6841 atcggcaaaa cggtggaaac cgaattgtcg ggcaacgtca ttgatttgtg tcccgtcggc
     6901 gcgctgacca gcaaaccgtt ccgcttcaac gcgcgtactt gggaattgaa ccgccgcaaa
     6961 tccgtttccg cccacgatgc tttgggcagc aacctgattg tgcagaccaa agaccatacc
     7021 gtccgccgcg tgttgccgtt ggaaaacgaa gcgattaacg aatgctggct gtctgaccgc
     7081 gaccgtttcg cctacgaagg cctgtatcac gaaagccgtc tgaaaaaccc gaaaatcaaa
     7141 cagggcggcg agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg
     7201 attgaatgta tcgccaaaga cggcaagcaa aaccaagtcg gcgtttgggc gaacccgatg
     7261 aatacggttg aagaactgta tctggcgaag aaactcgccg acggcttggg tgttaaaaac
     7321 tttgcaaccc gtttgcgcca acaagacaaa cgtctttcag acggccttaa aggtgcgcaa
     7381 tggttgggac aaagcattga atctttggct gacaacgatg ccgtattggt agtcggtgcg
     7441 aacttgcgca aagaacagcc gctcctgacc gcccgcctgc gccgcgccgc caaagaccgt
     7501 atggcattga gcgtattggc cagcagtaaa gaagaattgt ttatgccgct tctgtctcaa
     7561 gaagccgcac atcccgacga gtgggcaggc cgtctgaaaa acctgtctgc caatgcggaa
     7621 cacgccgtta ccgccagcct gaaaaacgct gaaaaagcag cggtgatttt gggcgctgaa
     7681 gtgcaaaacc atcccgatta cgccgccatt tacgccgccg cgcaagagct ggcagacgca
     7741 accggcgcag tgctgggtat tttgccgcaa gccgccaaca gcgttggtgc ggacgtattg
     7801 ggcgtgaact ccggcgagag cgttaccgaa atggcaaacg cgccgaaaca ggcagtcttg
     7861 ttgctcaacg tcgaacctga aatcgataca gttgacggtg caaaagccgt ggcagcgttg
     7921 aaacaggcga aaagcgtgat ggcgtttacg ccgtttgtca gcgaaacgct gctggacgtg
     7981 tgcgacgtat tgttgccgat tgcgccgttt accgaaacat caggcagctt catcaatatg
     8041 gaaggccgtc tgcaatcttt ccacggcgtg gtacaaggct tcggcgattc gcgtccgatg
     8101 tggaaagtgt tgcgcgtatt gggcaacctg tttgatctga aaggttttga ataccacgat
     8161 acctccgcga ttctgaaaga tgcgctggat gcagaaagcc tgccgtctaa actggacaac
     8221 cgcagcacat gggtagggga tggcgttcag acggcctcag accgcctcgt ccgtgtcggc
     8281 ggcgtcggta tttatcacac tgattctatc gtgcgccgtt ccgcaccgtt gcaagaaacc
     8341 agccatgccg ccgtgcctgc cgcgcgtgta aatccaaaca cattggcacg cttgggcctg
     8401 caagacggac aaactgttgt tgccaaacaa aacggcgcaa gcgtatcggt tgccgtcaaa
     8461 gctgatgccg gtttgcctga aaacgtggtg catctgccgc tgcataccga aaatgccgcg
     8521 ctgggtgcgt tgatggacac tattgaactg gcgggagctt gatcatgcaa gaatggttcc
     8581 aaaacctctt tgccgcaacg ctcggtctgg gcgatttggg tattaccgta ggcttggtgg
     8641 tatccgtcat cgtcaaaatc gtgattatcc tgattccgct gattctgacc gttgcctacc
     8701 tgacgtattt cgaacgtaaa gtcatcggct ttatgcagct tcgcgttggc ccaaacgtaa
     8761 ccggcccgtg gggtctgatt cagccgtttg ccgacgtgtt caaactcttg tttaaagagg
     8821 taacccgtcc gaagctgtca aataaagccc tgttctatat tggtccgatt atgtcgctag
     8881 ccccgtcttt tgcggcatgg gcagtgattc cgttcaacga agaatgggtg ctgaccaaca
     8941 tcaatatcgg ccttttgtat atcctgatga ttacctcgct gtcggtttac ggcgtgatca
     9001 tcgcgggctg ggcttccaac tccaaatatt cgttcttggg cgcaatgcgt gcttccgcgc
     9061 aaagcatttc ctacgagatt gccatgagtg ccgcgttggt gtgcgtcgtg atggtatcgg
     9121 gcagcatgaa cttctccgat atcgttgccg cgcaagccaa aggtattgcc ggcggttcgg
     9181 tattctcttg gaactggctg ccgctcttcc ccatcttcat cgtctatctg atttccgccg
     9241 ttgccgaaac caaccgcgca ccgtttgacg tagcagaggg cgaatccgaa atcgttgccg
     9301 gtcaccacgt tgaatattcc ggcttcgcat tcgcgctgtt cttccttgcc gaatacattt
     9361 tcatgattct gattgccgcg ctgacatcgc tgatgttcct cggcggctgg ttgtctccgt
     9421 tcccgcaaag ctggggcttt atcggtacgc cttccgcatt ttggatgttc gtgaaaatgg
     9481 cggcggttct gtactggtat ctgtggatac gcgccacctt cccacgctac cgttacgacc
     9541 aaatcatgcg cttgggctgg aaagtgctga ttccgatcgg cttcgcctac atcgtgattt
     9601 tgggcgtgtg gatgatttca ccgctgaatt tgtggaaata agtttcagac ggcatcttga
     9661 ggccgtctga acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa
     9721 tatggctaac ttagtaaaaa cctttctgct tggcgaattg gtaaaaggta tgggcgtaac
     9781 gctcaaaaac tttttcgccc gcaaagacac aatttatttc cccgaagaga aaacgccgca
     9841 atccgtgcgt ttccgcggtc tgcacgcgca gcggcggtat ccgaacggcg aagagcggtg
     9901 tatcgcgtgt aagttgtgtg aggcagtgtg tccggcaatg gcgattaaca tcgaatcgga
     9961 agaacgtgaa gacggtacgc gccgcaccaa gcgttacgac atcgacctga ccaagtgcat
    10021 cttctgcggt ttctgcgaag aggcatgccc gactgatgcg attgtggaaa cccatatttt
    10081 tgaataccac ggcgagaaaa aaggcgactt gcacatgacc aagccgattc ttttggccat
    10141 tggcgacaaa tacgaagctg aaatcgccaa acgcaaagcc gctgacgcgc cgtatcgtta
    10201 atgctttggg gcttcttgga aggttttaaa tatggaagga ctgattaatg cattgaaata
    10261 tttagccgaa catgagccaa tagataattt tgaagaaatt agaactagaa atagtccgat
    10321 tgagttgcca agtggattaa gtaattttga acaaaatatt tttttaaaag aaaatttatc
    10381 cccaaaatta caaaatgatg atagcttgaa gacgcattat tggattatcc gtgaatgggg
    10441 tgggattaaa agttttaaac aatctgctga aaatagccag cttattcgtc aatttttatc
    10501 ggaacttaat tcgggaaaat tgagtagtgg tttgttgaaa atttcatcat tatctaaatt
    10561 ggcttctttt atagattgtg agcgattcgc catttatgat tcacgcgcta ttttttcgtt
    10621 gaattggttg ttgtttaaat ttacaaatgc agatttgttt tttcagccac aaggtagaaa
    10681 tagggaacta gaaatccgaa atatgaacgt attgtttcat ttttctgata tcaaaccgaa
    10741 ttatcggaaa ccagacgttt cgtttcatca atattgtggg ttgttacaag atttggcgaa
    10801 acaagtttat ggtaaacaag caaaaccgta tcacatagaa atgttgttat tcaaaattgc
    10861 gacaacgtgg atttgtgcgg atatggatca actgattaag tttgattgtt tgcgtaacca
    10921 ggattttcag actgcttgaa accatatttt tgattaataa agaaagcata gactatgact
    10981 ttccaactga ttttatttta tatttttgca gtgataattc tttatggcgc gctcaaaacc
    11041 gtcaccgcta aaaaccctgt tcacgccgct ttgcatctgg tgctgacctt ctgcgtgagc
    11101 gcgatgcttt ggatgctgat gcaggctgag tttttgggcg tgacgctggt ggtggtttac
    11161 gtcggcgccg tgatggtgtt gttcctgttc gtcgtgatga tgttgaacat cgacattgaa
    11221 gaaatgcgtg ccggtttctg gcggcacgcg cctgttgccg gtgtggtcgg cacattgttg
    11281 gcggttgcgc tgatcctgat tctggtcaac ccgaaaaccg accttgccgc atttggtctg
    11341 atgaaagaca ttcctgccga ttacaacaat atccgcgatt tgggcagccg tatttatacc
    11401 gactatctgt tgccgtttga attggcggcg gtattgctgt tgttgggtat ggtggcggcg
    11461 attgcgctgg ttcaccgtaa aacggttaat ccgaaacgca tggatcctgc cgaccaagtc
    11521 aaagtacgcg ccgaccaggg ccgtatgcgt ctggtgaaaa tggaagcggt caaaccgcaa
    11581 gtcgaatctg ccgaagaaag cgaagtttca gacgacctca agccgaaaga ggagggcaaa
    11641 gcatgattac cttgacgcat tatttggtat tgggtgcgct cctgttcggt atcagcgcaa
    11701 tgggtatctt tatgaaccgc aaaaacgtgc tggtattgct gatgtcgatc gagctgatgc
    11761 ttttggcggt gaacttcaac tttatcgcct tctcgcaaca tttgggcgat actgccggac
    11821 aaattttcgt attcttcgta ttgaccgttg ccgctgccga atctgccatc ggtttggcga
    11881 ttatggtgct ggtgtaccgc aaccgacaaa caatcaacgt tgccgatttg gacgagttga
    11941 aagggtaaag gtaggttggg tcgagacctg acaagacacc gatgccgtct gaaaacccga
    12001 taggaaaaac gatgaaatcc atagacgaac aaagcctgca taatgcccgc cgcctgtttg
    12061 aaagcggcga catcgaccgt atcgaagtcg gtaccaccgc gggcctgcaa cagattcacc
    12121 gttacctgtt cggcggctta tatgattttg cgggtcaaat cagggaagac aacatttcca
    12181 aaggcggttt tcgttttgcc aacgccatgt atttaaaaga ggctttggtt aaaatcgagc
    12241 agatgcccga gcggactttt gaagaaatca tcgccaaata tgttgaaatg aacattgccc
    12301 atccgttttt ggagggtaat ggcagaagta cccgcatctg gctggatttg gtgctgaaaa
    12361 aaaacctgaa aaaagtcgtg aactggcaaa atgtaagtaa aaccctgtat ttgcaggcga
    12421 tggaacgcag ccccgtcaac gatttagaac tgcgctttct gttaaaggac aacctgactg
    12481 acgatgtgga caaccgtgaa atcatcttta aaggtatcga gcagtcgtat tattacgaag
    12541 ggtatgaaaa aggctgaggg tcgtctgaaa agcgatttca gactgtttca gacgacctga
    12601 ttcggtaggt gatcagacgg gagcggatga gaaaagaaat tctgggtaag aataatccgg
    12661 tctgaaatat tggaagaaga atgatggata aaaatcagtt agaacaagaa tttcataaag
    12721 ccatgttaaa tatttatcag gaggctttga atttgccgca accttacaag gcgacacgat
    12781 ttttacaaat tgtaaatgaa tttggtggta aagaggcggc ggataaatta ttgagtacgg
    12841 gggaaaagaa gactcagacc ggttttacag agctgatttt gagtggtggc ggagtccacg
    12901 ccttgaaata cagtatggaa tatctggtgt tacaaaagcc gtggtgtgat ttatttactg
    12961 aagagcaatt agctgtggca cgcaaacgat tggagcgtgt tggatttgtt tttccgaagt
    13021 aattttgtac gaaacaaaca tagattttta aatcaatcgg attcaatcaa atgaacgata
    13081 tgactttata tttgataatt gcccttgttc cgttggcagg ctcgctgatt gcgggtttgt
    13141 tcggcaacaa aatcggacgt gccggtgcgc atacggttac gatactcggc gtggcggtgt
    13201 ccgccgtgct gtcggcttat gtgctgtggg gctttattga cggcagccgc gccaagtttg
    13261 acgagaatgt ctatacctgg ctgacaatgg gcggcttgga tttctccgtc ggcttcttgg
    13321 tcgatacgat gacggcgatg atgatggtcg tggtaacggg cgtgtcgttg atggtgcata
    13381 tctataccat cggctatatg cacgatgaaa aagtcggcta ccaacgcttc ttcagctata
    13441 tttctttgtt tacattcagt atgttgatgc tgattatgag caacaacttc attcagctct
    13501 tcttcggttg ggaagcggtg ggcttggtgt cgtatctctt gatcggtttc tatttcaaac
    13561 gcccgagcgc gacatttgcc aacctgaaag cctttttgat caaccgtgtc ggcgacttcg
    13621 gctttttgct cggtatcggc ttggtgcttg cctatttcgg cggcagcttg cgctatcaag
    13681 atgtattcgc ttatctgccc aacgtgcaaa atgccactat ccaactgttc cccggtgtgg
    13741 aatggtcttt gattactgta acctgtttgc tcctgtttgt cggtgcgatg ggtaaatcgg
    13801 cacaattccc gctgcacgtc tggctgcctg attcgatgga aggcccgacc ccgatttctg
    13861 cattgattca cgccgcaacc atggttaccg ccggtttgtt tatggtgtcg cgtatgtcgc
    13921 cgatttatga aatgagcagc accgcgctgt cggtcattat ggtgatcggc gcgattaccg
    13981 ccctgtttat gggctttttg ggcgtgattc aaaacgacat caaacgtgta gttgcgtatt
    14041 ccaccctgtc gcaattgggc tacatgaccg tggctctggg cgcgtctgcc tattccgtgg
    14101 cgatgttcca tgtgatgacc cacgccttct ttaaagccct gttgttcttg gcggcaggca
    14161 gcgcgattat cggtatgcac cacgaccaag acatgcgcca tatgggcaat ctgaaaaaat
    14221 atatgccggt tacttggctg accatgctga tcggtaactt gtcgctgatt ggtacgccgt
    14281 tcttctccgg cttctactcc aaagattcga ttatcgaagc ggcgaaatac agcacactgc
    14341 cgggcagcgg ctttgcctat tttgccgtcc tcgccagcgt gtttgttacc gcgttttacg
    14401 cgttccgcca atactttatg gtgttccacg gcgaagagaa atggcgcagc ctgcccgaac
    14461 accattcaga cggccacggc gaagaacatc acggtttggg taaaaacgac aatccgcacg
    14521 aaagcccgtt ggtggttacc ctgcctttga ttttgcttgc cgttccgtcc gtcatcatcg
    14581 gctacatcgc catcgaaccc atgctctacg gcgatttctt caaagacgtg attttcgtca
    14641 acgccgacgc gcatccgact atacacatca tgaaggaaga gttccacggc gcattggcaa
    14701 tggtgtccca cagcctgcat tcgcccgtac tctaccttgc tatcgcaggc gtgttgagcg
    14761 catggctttt gtacgtcaaa ctgccgcacc tgccagcgaa aattgcacag acgttccgtc
    14821 cgatttacgt tttgtttgaa aacaaatact acctcgacgc cctgtatttc aacgttttcg
    14881 ccaaaggcac acgcgcattg ggcactttct tctggaaagt cggcgatacc gccattattg
    14941 acaacggtat tgtcaacggc tctgccaaac tggtcggcgc gattgccgcg caagtgcgta
    15001 aagcccaaac cggctttatc tacacctacg ccgccgctat ggtgttcggc gtattggtct
    15061 tgctcggcat gaccttctgg ggattgttcc gataagaata aggtttcaga cggccttaaa
    15121 ccttcaggcc gtctgaaacg aagaaatatc cacataaaca catttttatt ttaaccacag
    15181 gttaaccact atgttttcca actacctact cagcttggca atatggatac ccatcgccgc
    15241 aggcgtgctg gttttggcaa cggggtcgga cagccgtgcg ccgtttgccc gcgtgctcgc
    15301 cttcatgggt gcgcttgccg gtttcttggt aacactgccc ctgtttaccg gtttcgaccg
    15361 tttgagcggc ggctatcaat ttaccgagtt ccacgagtgg attccgcttc tgaaaatcaa
    15421 ctacgcattg ggcgtggacg gtatttcagt gctctttatc atcttgaatg cgtttattac
    15481 gctgttggtg gtattggcag gttgggaagt cattcagaaa cgtccggcgc agtatatggc
    15541 ggcattcctg atcatgtcgg gtttgattaa cggcgcgttt gccgcgcagg atgcgattct
    15601 gttttatgtg ttcttcgagg gtatgctgat tccgctgtac ctgattatcg gtgtatgggg
    15661 cggtccgcgc cgcgtctatg cgtcggtcaa gctcttcctc tacacgctga tgggttcgct
    15721 cctgatgctg gttgcgatgg tttaccttta ttatcaaaca ggcagcttct ctattgtcga
    15781 tttccaaaac atcgaacaga ttccgttggg cgtacaacag cttttgtttg tggcgttctt
    15841 cctgtcattt gccgtaaaag tgccgatgtt ccctgtgcac acttggttgc cggatgccca
    15901 cgttgaagcg ccgaccggcg gttcgatggt gttggcggcc attacgctga aactgggtgc
    15961 gtatggtttc ttgcgcttta tcctgccgat tatgccggat gcggcacgct attttgcccc
    16021 cgtgatcatc gtattaagtc tgattgccgt gatttatatc ggtatggtgg ctttggtgca
    16081 aaccgatatg aaaaaactgg tggcgtattc gtccatcagc catatgggtt ttgtaacgct
    16141 tgggatgttt ttgtttgttg acgggcagtt ggacgactgg gcattgaaag gtgcaatcat
    16201 tcaaatgatt tcgcacggtt tcgtgtctgc cgcgatgttt atgtgtatcg gcgtgatgta
    16261 cgaccgcctg cacacgcgca atattgctga ttatggcggc gtggtcaatg tgatgcccaa
    16321 gtttgcggcg tttatgatgc tgttcggtat ggcgaacgcg ggtttgcctg cgacttccgg
    16381 cttcgtgggc gagtttatgg tgattatggg cgcggtcaaa gtgaatttct gggtcggcgc
    16441 gttggccgcc atgaccctga tttacggtgc atcttatacc ctgtggatgt acaaacgcgt
    16501 tatttttggt gcgatccaca atccgcacgt tgccgaaatg caagacatca attgccgcga
    16561 atttgcgatt ttggcaattt tggcggtggc tgttttgggt atgggcctgt atccgaacgc
    16621 atttatcgaa gtggtgcatc aggcggcaaa cgatttgatt gcccatgtgg cacaaagcaa
    16681 gatttgaggt gtgtaaatga actggtctga tttgaattta atgcccgcca tgcccgaaat
    16741 cgtgctgctg tcgctgctgg tgttattgtt gctggcggac ttgtgggtca gtgatgacaa
    16801 acgccggtgg acgcattacg gcgcgttggc aacggtggcg gttacggctg tggtgcagtt
    16861 ggcggtgtgg gaacagggca gcacgtcttc gttcaacggg atgtatattg cagacggtat
    16921 gtcgcgtttg gcaaaaatgg ttttatatgc cttgaccttt gccctgtttg tctatgccaa
    16981 gccctacaac caagtgcgcg gtatttttaa aggcgagttt tacaccctgt cattgtttgc
    17041 cctgttgggt atgagtgtga tggtgagcgc ggggcatttt ttaactgcct atatcggttt
    17101 ggaactcttg tcgcttgccc tttacgccct gattgccctg cgccgcgatt ccggctttgc
    17161 cgccgaagcc gccttgaaat attttgtttt gggcgcgctg gcatccggcc tgctgctcta
    17221 cggtatttct atggtttacg gcgcaaccgg ttcgctggaa tttgccggcg tgctcgcctc
    17281 ttccttcaat gaagaagcca acgaatggct gttgaaactg ggtttggtgt ttatcgtcgt
    17341 cgccgtcgcg ttcaaactcg gtgcggtgcc gttccatatg tgggtgcccg acgtgtatca
    17401 cggcgcgccc acttctgtta ccgccttggt cggcactgcc ccgaaaatcg ccgccgtcgt
    17461 tttcactttc cgcatcctcg ttaccgggct gggaaccgtg catcatgact ggtctctgat
    17521 gtttgccctg cttgccgccg cctcgctgct ggtcggcaac cttgccgcca tcatgcagac
    17581 caatatcaaa cgtatgttcg cctattccac cgtatcgcat atgggtttca tcctgttggc
    17641 gtttatggcg ggcgcggtcg gctttgcggc gggcctctat tacgccatta cctacgcgct
    17701 gatggcggcg gcagggttcg gagtgttgat ggtgttgtcg gacggggaca acgagtgcga
    17761 aaacatcagc gatttggcag ggttgaacca acaccgcgta tggcttgcct ttttgatgct
    17821 gctggttatg ttctctatgg cgggcattcc gccgctgatg ggtttttacg ccaaattcgg
    17881 cgtgattatg gcactcttga aacaaggcca tgtttggttg tctgtatttg ccgtcatcat
    17941 gtcgctgatt ggtgcgttct actacctgcg cgtggtcaaa gtcatctact tcgatgtgcc
    18001 tgatcatgac cagccggtcg gcagcaacta tgccgccaaa tttgttctga cggtcaatgc
    18061 cttcttgctg ctcctgtggg gcatcatgcc gcaaaccgtt atcgactggt gcgccaaggc
    18121 gttggagaac acgctgtaag ccgccgcaac ggcagccgtg tcagaggctg ccgtttttgt
    18181 taagatatgc cgttccgcaa cgcggttcag acggcatcgc cgccgacaac gcctaaacag
    18241 aaagcccacc atgaccgcat ccatgtacat ccttttggtc ttggcactca tctttgccaa
    18301 cgcccccttc ctcacgacca gactgttcgg cgtggccgca ctcaagcgca aacatttcgg
    18361 acaccacatg atcgagctgg cggcaggttt cgcgctgacc gccgttcttg cctacatcct
    18421 cgaatcccgt gcaggatcgg tacacgatca gggttgggag ttttatgcca cagtcgtctg
    18481 cctgtacctg atttttgcgt ttccatgttt tgtgtggcgg tatttttggc acacgcgcaa
    18541 cagggaatag acaagcatag gaatgccgtc tgaaaccctt tcagacggca tttgtttcat
    18601 tcaagtgcag gccggcatcg ctgtgccggc acgtttcagc cggcgatata cgccggtttt
    18661 aatatttgcg ggcgactgca aattctgcca actgccgcag gcgcagggct ttgtcgccga
    18721 agggttcgag cagcgcgacc gcttcggcaa ccagtttgtg tgcgtatgag cgcgccgctt
    18781 ccaagcccat cagtttcaca taagtcggct tgtcgttgtc tgcgtctttg cccgccgttt
    18841 tgcccaaagt cgccgtgtcc gcttcacaat ccaacacatc gtcaatgact tggaacgcca
    18901 gccccagttt tgccgcgtaa gcgtccaata cggaaagttc cgcatctgac agatcaggac
    18961 acgccgtcgc ccccaataaa accgccgcac ggattagcgc acccgttttc aggctgtgca
    19021 tctgttccaa atcggcttga accatttgtt tgccgacatt cgccaaatcg attgcctgac
    19081 cgcccgccat acccctgctg ccgcccgctt tcgccaacac cgacaacatt gccaactggc
    19141 gtgcggcggg cagttctgtc ggacggctca acacgtcaaa tgcctgtgtc tgcaaagcgt
    19201 cgccggtcag aagggcggtc gcttcgccat atttgatgtg gcaagtcggt ttgccgcgcc
    19261 gcaggctgtc gttgtccatc gccggcatat cgtcgtgaac caaagaatag acgtggatca
    19321 tttcgattgc cgccattgcc tgttctactg cttcatgcac ggcttcgcct aattccgaag
    19381 ctgccagaac cagcatcggc cgcagacgct taccgccgtc caaagccgca taacgcatcg
    19441 cttcgtgcag tgtgtgcggt atttccccct cagacggtaa aaaccgttca agcagcagct
    19501 ctgtttgcgc ctgcgccctc tgttgccacg ttttcaaatc attcgtcgga ttcaaggttt
    19561 aactccttca gcccgtctgt gtctaaaacc tgtagctttt gttcgacttg tgccagtttg
    19621 gtttggcagt acctgaccag ttcgttgcct tcctgatagg cggcaagcgc gtcttccaag
    19681 ggcatttcgc cctgcataga ctgcgtcagc gattcgaggc gcgacaaggc ttcttcaaac
    19741 gatttcgggg cgtttttctt catcgtattt ccttttcggt tgaaaccccg ccctttaggg
    19801 cggcaggatc agactttatt tgggaggggt gtaacccttt ccaaatcagg gcaatacata
    19861 gggcggtgct ttatgtgccg tcctgtgtgt tggaacatag tttcggatgt tccggtaaaa
    19921 agcggattgt agcatttttg aaaaacggat gccgtctgaa acccgaatcc ggcttcagac
    19981 ggcatttttt ccgcccaggc ggcaaggcgt tacccgggca gttcgtcggt gatgccctgc
    20041 aaaaaggcga ggcgttcggg gcttgccgcc ccggtttgcg cggcggcttt gaaggcgcag
    20101 ccgggttcgg cgcggtgggt gcagttgtgg aagcggcatt gcccgacaag gtggcggaaa
    20161 tcggggaaat agcgcggcaa atcggcggct tggaggtggt gtaaaccaaa ttcttgcaaa
    20221 cccggggagt cgatgagttg ggtttcgccg ttcaaatcat aaagccgggc gtgggtggtg
    20281 gtgtgttttc ccgagtcgag tgcggcggaa atgtcgccgg tgcgggcggt ttggctgccc
    20341 aaaagggcgt tggtcagggt ggatttgccc ataccgctct gcccgagcag gatgttgctg
    20401 tgcccttgca gggcggggcg caggctgccg gcgttttcca gtgcgcgggt ttcgatgacg
    20461 ggataaccca gcgtttcgta gaatttgagt ttttcgcgcc aaagggcggt ttcgggcagg
    20521 tcggctttgt tcaggacgat gacggcttca ataccggcgg cttcggcggc aagcagggcg
    20581 cgttgcagca gccgcacgct cggactcggg acggcggcgg ttacgatgag gagttgggta
    20641 acgttggcgg cgatgagttt ggttttccac gcgtcttggc ggtagagcag gctttggcgc
    20701 ggtaaaaaat cttcaatcac aacttgttcg gcgttgacgg ggctgatgcg gacgcggtcg
    20761 ccgcaggcga aatcgacgcg ttttttgcgg gtgctggctt cgtaggttgt gccgtcgggc
    20821 gtgcggacaa tgtagcggcg gccgtagctg gcggtaattt gggcggtgtc gttcatggtt
    20881 tctttggggt tgggtgtggg aatgccgtct gaaaacgggt gttcggacgg catcggttca
    20941 gtcgtgctgc cactcgacgt gttcgttgag gaagccgccg ctctggtgcg cccagagttt
    21001 ggcgtaaagc ccgcgttttt cgaggagttc ggcgtgtgtg ccttcttcga tgatgcggcc
    21061 tttgtcgagg acgacgagcc tgtccattgc ggcgatggtg gagaggcggt gggcgatggc
    21121 gatgacggtt ttgccgtcca tcattttgtc gaggctttct tggatggcgg cttcgacttc
    21181 ggaatcgagc gcgctggtgg cttcgtccaa aagaagaatc ggtgcgtctt tgagcatcac
    21241 gcgggcgatg gcgatgcgct ggcgttgccc gccggagagt ttcacgccgc gttcgccgac
    21301 gtgtgcgtcg tagccgcgcc gccctttggc atcggaaagg tcggggatga agccggcggc
    21361 ttcggcgcgt tcggcggcag aaaccatttc ggcatcggtc gcgtcggggc ggccgtaaat
    21421 aatgttgtcg cgcacggaac ggtgcagcag cgaggtatct tgcgtgacca aaccgatttg
    21481 ggcgcgtaaa gattcttggg taacgccgct tatgtcctgc ccgtcgatcg aaaccgtgcc
    21541 gctttgcggt tcgtagaagc gcaaaagcag gttgacgatg gtggatttgc ccgcgccgct
    21601 gcgtccgatc aagccgactt tttcgcccgg gcggatggtg aggttgaagc cgttgagcag
    21661 cggtttgccc gcttcgtagg agaaatcgac gtgttcaaat ttgattgcgc cttgcggcac
    21721 gttcagcggc agtgcccggg gcttgtcgag gatggtgtgc ggtttggaca gggttgccat
    21781 gccgtcgccg acggtgccga tgttttcaaa cagccgcgcg gattcccaca taatgtattg
    21841 cgacaaaccg ttgacgcgca acgccatggc ggtggctgta gcaaccgcgc ccacgccgac
    21901 ctgcccgttg tgccagagcc agatgcccag tgcggcggtg gagagggtca gggaggtgtt
    21961 gacgatgaag ctgcacgaat gcagcagcgt cgccagccgc atttgggcgc gcaccgtaac
    22021 cataaattct tccatcgact gcttggcata ggcggcttca cgcgcgccgt gggagaagag
    22081 tttgacggtg gcgatattgg aataggcatc ggtaatgcgg ccggtcatca gcgagcgggc
    22141 atccgcctgc catgcggcgg tttgccccaa tttgggaatc agcaggcgca tcaccgaagc
    22201 gaaaccgaca atccagccga taaagggcag cagcagccat gagtcgagcg aggcgagaat
    22261 cacgccggag gtaatgaaat acaccgacac ataaacgacc atatcggcaa ccgtcatcac
    22321 cgcgtcgcgc aacgccagcg cggtctgcat gactttggcg gacacgcgtc cggcaaattc
    22381 gtcctgataa aaaccgaggc tttggttcag catcaggcgg tggaagttcc agcgcaggcg
    22441 catggggaac acgccctgaa gggtttgcag gcgcacgttg gacgcggcaa acgcccacgc
    22501 aaccgaaaat accatcatcg ccgccattgc cgccagttcc caaccttttt cggcaaacag
    22561 ttcggcgggc gcgtatttgc cgagccactc cacgattttg cccataaatt gaaaaaccag
    22621 ggcttccata atgccgatgc cggcggtcag cgcagccagg gcggctatcc atttccgcac
    22681 gccggccatg ctgctccaga caaaccgcca caagcctttt tctggcgttt tcggggcggc
    22741 ttcgggataa gggtcgattc gggactcgaa ccaggaaaat attttgttca acattgtttt
    22801 cgatttcggt aaaacagttt cagacggcat caaacacaat gccgtctgaa aggaaggaca
    22861 ataacgccat tttacgggaa aagccgtcgg gaagacagcg cgaggcggaa acgcagggtt
    22921 tcgtcagggc aaacgccgcg ccgccttcag gcggcattat ttcagcaggt ttttcaaagc
    22981 aaggcgcacg ccttcgccca cgtccgtccc ctccggaacg cctttgaccg ccgcttttgc
    23041 ttcgcgttcg ctgtaaccca gcgcaagcag cgtgctgacg atgtcttccg tttcgtcggc
    23101 ggcgggtgcg gcggcaaaca gcccgtccgt taccgtatgc gcgaccagct tgccgcgcag
    23161 ttccaaaacc atacgttcgg cggttttttt gccgattccc ggggcggagg agaggcgttt
    23221 gacatcttct tctgcaaccg cccgcgccag ttcgtcggca gtcattgccg acaaaatgcc
    23281 caaagccgtt ttcgcgccga tgccgccgac cttgatcagt tggcggaagg tcttgcgttc
    23341 ttccgcagtg gcaaaaccaa ataaaagatg tgcgtcttcc cgaatgataa gctgggtaaa
    23401 cagttgtacg ctttcaccca cgggcggcag gttgtagaag gtctgcatcg atacgtcggc
    23461 ctcatagccg acaccgttga catcgatgac gatttgcgga gggttttttt caaccagttt
    23521 gccggtcagt ctgctgatca tgtgtgccga atcctgaagt gtcgggtgca aaatgccgtc
    23581 tgaaaccggt ttgggcttca gacggcacgg attgtatcaa attcagtcgt cgcggcggga
    23641 ggaaatcacg cggccggtac gggcatcgac aacgactttg tattcctgtc cgtttttgac
    23701 gatttcgaca tcatagtgcg gacggccgtt gtcgtgttcg agatcgatgt cggtgatttt
    23761 gccgccgaca cgcgccaacg ctgctttttc ggcttgggcg cggctgatga ttttgtcttg
    23821 tttgttgtgt tggtgtgcgg cgtgtccgtg gtcgtcatcg ccgtgtccgt cgtggtgggc
    23881 gagcgcgggg gcggaaatgc tcagcagtgc ggttgcggcg gaggtcaaga gaaggtgttt
    23941 gatgttcata ttttgccttt gtaaatcgtg ggttggaaaa tgtggatatt aataaggtat
    24001 caaataaccg tcagccggcg gtcaataccg cccgaaccat accgcgcgcc tgagcttcgg
    24061 cttcggcggc gcgttcctgc gaggtaaacg gtcccatttt gacgacgtat tcgtaacggc
    24121 gtttttcaac cgagaggttc gtacccgatg acgaaacggc gaagttttgg gcggcttggt
    24181 tcagataggc ttgtgcttcg tgttccgtac cgaaagattt caagtcgata aagatgtctt
    24241 tgttttcggc aaccggtgcg gattggcccg ggacgatttg ttcgattttg acgtgtgccg
    24301 tcccttggtt gacaaagccc aatttttgcg cggcggcttt ggatacgtcg atgatgcggt
    24361 tgccgtggaa ggggccgcgg tcgttgacgc ggacgatgac gcttttgccg tttttggtat
    24421 tggttacgcg cacatagctg gggatgggca gggttttgtg ggcggcggta aaggcgttca
    24481 tatcgtatcg ttctccgccg gaagttttgc gcccgtgaaa cctgccgccg taccacgagg
    24541 cgttgccggt ttgcgtgaat tcggcgactt ggtttttcgg cgtgtagcgt tttccggcga
    24601 ctttgtagct gcggttggcg gaggcgtgca gtttttctgc cttgaccact gcgtcggcgg
    24661 atgccgtctg aagggagtgt gtgccgaatg cggcggtgag aaggaaaagg gtttttcggg
    24721 ttaaagtcaa aacgtgttcc gttcttgagt tgaagacgaa tgggcatcat gcccgccgga
    24781 tacgttccga accgccgtac agtgcggacg gcggttcgga atgtgtccgg ataggttttc
    24841 agacggcatg aacctgcgtt caaacgccgc ctgcgtaacc gtgttgccgc cacgcttcaa
    24901 agagaatcac ggcgacggtg ttggaaaggt tcatactccg gctgccgggc tgcatcggca
    24961 ggcggatttt ttgcgcggcg ggcaggctgt cgaggatgtc ggcaggcagt ccgcgcgttt
    25021 ccggcccgaa cagtaaaacg tcgccttttt gaaacgcggt ttcatcgggg cgcgccgtgc
    25081 ctttggtggt cagggcgaaa atgcgcctgc ctgcgagtgc cttgaggcag tcgtcgaagt
    25141 tttcgtgcac cgtcaggctg gcgaactcgt ggtagtcgag cccggcgcgt ttcattttgg
    25201 cggaatccaa tgggaagccg agcggtttga caaggtgcaa atccgcgccg gtattggcgc
    25261 acaggcggat gatgttgccc gtgttcggcg ggatttccgg ctggtataaa acgatggtaa
    25321 acataaatat caatcactta taggcgcgta accttgccac aaggcggatg gggtgtcaaa
    25381 aaatttagtt attttttcat tggcgtgcgt gccagcgtcc agcagcagat tcggtttgcg
    25441 cccgattttt tcagcgtctt tgccaattcg tccagcgtcg cgccggtggt aaagacatcg
    25501 tcgattaaca gaatattaca gttttccggt atcggtgtgc ggatttcaaa ggcgtttttg
    25561 atgtttcgcc gccgttcgcc gcctttgagc gtgctttgcg gcgggcggtg gtgtcggaaa
    25621 acggtgtgtc ggggcagtat ctgccagccg tagcgttgtg ccagcagccc gacgatgctt
    25681 tcactttggt tgaacccgcg ttgcagcagc cgctccctgc ttagcggtac gggcaggacg
    25741 aaatcgaaac attcgtctgc aagccggtcg ggcggattct gcatcatcag gtctgccagc
    25801 ggctgcacca tgctcaaatc agccaagtgc ttcagcgcgt gtatcatatt gctgacgggc
    25861 ggttcgtaat gcagcgaagc ccacatccgg tcgaatgcgg gcggtttttt ctgacagccg
    25921 ccgcacaccg atccgccttg gatgtgtctg aaacacaggg ggcagctgtt tgccgcgtcg
    25981 gtgcggtatg ccgccaaatc gtcgcggcag ccggcgcaga tgccgtctga aacgccagac
    26041 gaaccgtggc ataatacgca acgcctgata gtgggcgcgt ctgcgatgcg ccgccaacga
    26101 gagagaaaat ccatgcctga tgccgtcaaa aaagtttacc tgatacacgg ttggggggcg
    26161 aaccgccaca tgttcgacga tttgatgccg cgcctgcctg caacgtggcc ggtgtccgcc
    26221 gtcgatttgc ccggacacgg ggacgctccg tttgtccgac ctttcgacat tgcggctgcg
    26281 gccgacggca ttgccgctca aattgacgct ccggccgaca ttctcggctg gtcgctcggc
    26341 ggattggtcg cgctgtatct ggcggcgcgc catcccgaca aagtccgttc gctctgcctg
    26401 acggcgagtt tcgcacggct gacggctgac gaagactatc ccgaagggct tgccgcgcct
    26461 gcattgggca aaatggtcgg tgcgttccgt tcggattatg ccaaacatat caaacagttt
    26521 ctacaattac agcttctgca cacgcctgat gcggacggaa tcataggcag aatcctgccc
    26581 gatttggcgc gctgcggcac gcctcaagcc ttgcaggagg cgttggacgc ggcggaaagg
    26641 gcggatgcgc ggcatttgtt ggacaagata gatgttccgg tactgctggt gttcggcggc
    26701 aaagacgcga ttacgccgcc gcgtatgggt gaatatctgc accgccgttt gaagggcagc
    26761 aggttggttg tgatggaaaa ggcggcgcat gcgccgtttt tgagccatgc ggaagcgttt
    26821 gccgcgctgt accgcgactt tgttgaaggg ggtttgagat gaaccatcag gacgcacgct
    26881 ggcaggttca ccgccatctt gccgaacata ccgaccaacg gctgacactc gtccgcaacg
    26941 cgcccaagca tatcctgctt gccggtgcgg atgcggacat cagccgcagc ctgctggcga
    27001 aacgctatcc gcaggcggta tttgaagaat acgattcccg tgcggatttt ttggcggctg
    27061 ccgctgccgc ccgcaaaggc ggtttttggc aaaggtttac gggtaagggc gtggtgcaac
    27121 actgccaatc cccgatcgcg ccgctgcccg aagcgtgtgc cgatatgttg tggtcgaatc
    27181 tcggactgtt ggcggcggaa caaatccttc ctgtgctgca caactgggcg cgcgccttga
    27241 agacggacgg gctgctgttt tttacctgct tcgggcgaga taccttggcg gaactgaaat
    27301 gccgtctgaa agaaaacggc attgaaagcc gcagcgcgct tttccctgat atgcacgact
    27361 tgggcgatat gcttgctgaa aacggctttt acgaccccgt taccgatacg gcgaagctgg
    27421 tgttggatta caaaaaggcg gaaacgtttt gggcggatat ggacacgctg ggcgtttggc
    27481 gggcgatggc gtggaacgat gaaaacgccg cgcgttcgtg tgtcgggaca atatttgagc
    27541 gggaaggcgg tttgggcatt acgctggaaa cggtgtacgg acacgccgtg aaaaaactga
    27601 tgctgccgca aggggagaac gtggtgcagt tttttccgaa gagatgatgt gcagatgccg
    27661 tctgaagccg tttccaggtt tcagacggca tttgtctgtg aaaaccgaca gaaataaagg
    27721 aaatgccgat gtatagtg
//
LOCUS       DAWISC010000007        26418 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWISC010000007 DAWISC010000000
VERSION     DAWISC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.007"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            673..2121
                     /gene="gnd"
                     /locus_tag="WIW87_000216"
     CDS             673..2121
                     /gene="gnd"
                     /locus_tag="WIW87_000216"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ5173437.1"
                     /translation="MNGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTTAKVDEFLNG
                     AAKGTNIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDEFIEQLLPLLEEGDILIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWDAVKPIFQAIA
                     AKTSQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMYRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2184..3455
                     /gene="waaA"
                     /locus_tag="WIW87_000217"
     CDS             2184..3455
                     /gene="waaA"
                     /locus_tag="WIW87_000217"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ5173438.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQSLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMRECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDIIPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGYRGR"
     gene            3496..3945
                     /locus_tag="WIW87_000218"
     CDS             3496..3945
                     /locus_tag="WIW87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ5173439.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3978..4949
                     /locus_tag="WIW87_000219"
     CDS             3978..4949
                     /locus_tag="WIW87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ5173440.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNTVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASVLTSLVYTKKTGR"
     gene            5023..5098
                     /locus_tag="WIW87_000220"
     tRNA            5023..5098
                     /locus_tag="WIW87_000220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5056..5058,aa:Lys,seq:ttt)
     gene            5469..6722
                     /gene="murA"
                     /locus_tag="WIW87_000221"
     CDS             5469..6722
                     /gene="murA"
                     /locus_tag="WIW87_000221"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ5173441.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELQGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6803..8014
                     /locus_tag="WIW87_000222"
     CDS             6803..8014
                     /locus_tag="WIW87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ5173442.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGSLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIAADAEAVVKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQ
                     FAGGTKALAEAIAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKEL
                     PAVAALEKRGA"
     gene            complement(8081..8329)
                     /locus_tag="WIW87_000223"
     CDS             complement(8081..8329)
                     /locus_tag="WIW87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ5173443.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8420..9337)
                     /gene="ftsX"
                     /locus_tag="WIW87_000224"
     CDS             complement(8420..9337)
                     /gene="ftsX"
                     /locus_tag="WIW87_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ5173444.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLMMLAVAMTLPLF
                     MHLGIQSGQSVLGKLNESPQITIYMETSAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYAGELG
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9334..9984)
                     /gene="ftsE"
                     /locus_tag="WIW87_000225"
     CDS             complement(9334..9984)
                     /gene="ftsE"
                     /locus_tag="WIW87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ5173445.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTI
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10178..10660)
                     /locus_tag="WIW87_000226"
     CDS             complement(10178..10660)
                     /locus_tag="WIW87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ5173446.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTDAVKLAHSKCR
                     "
     gene            10784..11137
                     /gene="arsC"
                     /locus_tag="WIW87_000227"
     CDS             10784..11137
                     /gene="arsC"
                     /locus_tag="WIW87_000227"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ5173447.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11134..11778
                     /locus_tag="WIW87_000228"
     CDS             11134..11778
                     /locus_tag="WIW87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ5173448.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            11983..13377
                     /locus_tag="WIW87_000229"
     CDS             11983..13377
                     /locus_tag="WIW87_000229"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ5173449.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGSATYDRRWRPEVGKTLPEIPSDVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIDANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLELVKDRPQDLNTLADECFYFYVKQTPAEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13398..13793
                     /locus_tag="WIW87_000230"
     CDS             13398..13793
                     /locus_tag="WIW87_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173450.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEDLRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13797..14288
                     /locus_tag="WIW87_000231"
     CDS             13797..14288
                     /locus_tag="WIW87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5173451.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSAAVKRHDMESFPRYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14412..17006)
                     /gene="mutS"
                     /locus_tag="WIW87_000232"
     CDS             complement(14412..17006)
                     /gene="mutS"
                     /locus_tag="WIW87_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ5173452.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHTAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17450..18454
                     /gene="gap"
                     /locus_tag="WIW87_000233"
     CDS             17450..18454
                     /gene="gap"
                     /locus_tag="WIW87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ5173453.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVASGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18524..18643)
                     /locus_tag="WIW87_000234"
                     /pseudo
     CDS             complement(18524..18643)
                     /locus_tag="WIW87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18712..19392)
                     /gene="tsaA"
                     /locus_tag="WIW87_000235"
     CDS             complement(18712..19392)
                     /gene="tsaA"
                     /locus_tag="WIW87_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ5173454.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19506..20141
                     /locus_tag="WIW87_000236"
     CDS             19506..20141
                     /locus_tag="WIW87_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ5173455.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20178..21146
                     /gene="waaC"
                     /locus_tag="WIW87_000237"
     CDS             20178..21146
                     /gene="waaC"
                     /locus_tag="WIW87_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ5173456.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21373..22122
                     /locus_tag="WIW87_000238"
     CDS             21373..22122
                     /locus_tag="WIW87_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ5173457.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22133..23068
                     /locus_tag="WIW87_000239"
     CDS             22133..23068
                     /locus_tag="WIW87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ5173458.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23131..23742
                     /locus_tag="WIW87_000240"
     CDS             23131..23742
                     /locus_tag="WIW87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ5173459.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23794..24111)
                     /locus_tag="WIW87_000241"
     CDS             complement(23794..24111)
                     /locus_tag="WIW87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ5173460.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24269..25540
                     /gene="purD"
                     /locus_tag="WIW87_000242"
     CDS             24269..25540
                     /gene="purD"
                     /locus_tag="WIW87_000242"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ5173461.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25574..26176
                     /locus_tag="WIW87_000243"
     CDS             25574..26176
                     /locus_tag="WIW87_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ5173462.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6344 a         6081 c         7441 g         6552 t
ORIGIN      
        1 tcatcattcc cactttcgtg ggaatgacgg aatgtaggtt cgtgggaatg acgcggtaca
       61 ggtttccgtt acgtatcccc atccgaaagt ttacttatac acaagcaatc caaagttatc
      121 tgactgaaat attgtgagat ttacccgcaa atctcatttt ttccaccact tatacacaaa
      181 ttcggatgcg cactctgccg atatgcagaa ataagacatt cgtgccgaca cctgaccatt
      241 tccacaagtt ccaaacacac tttcctctca aacctccaca gtattccggt tcttaaagac
      301 atcggcagcc attcggtaaa gaaaagtagg atgccatttg cacaacaagc aaactgtcca
      361 caaaaactat tcttcatgaa aaacaatcca caggtatgca caaaagggaa tccgccttac
      421 cgacacccta aaaaaaagaa atcctgatga tttttaaaat aaaaaaaagt tacccacaga
      481 aaatttctcc tatcaatata atcatcgttt tattatttaa attttatatt ttatttataa
      541 cccttcagca aaacggtaca gcaaagcgta aaggcatctt ttcaaaaaat taaaaagtta
      601 aggattgtta aacaagtgta aatcgggtag agttgcggtc gataaattaa atctataacg
      661 gagcttcctg caatgaacgg cgatattggt gtaatcggtt tggcggttat ggggcaaaac
      721 ctgattttga atatgaacga ttgcggattt aaggtggttg cctacaaccg cacgactgcc
      781 aaggtggacg aatttttaaa cggcgcggca aaaggaacta atattatcgg cgcgtattct
      841 ttgcaagatt tggtcgataa gctggaaaaa ccgcgaaaaa ttatgatgat ggttcgtgcc
      901 ggttcggtgg ttgacgaatt tatcgaacaa ctgcttccgc tgttggaaga aggcgacatc
      961 cttatcgacg gcggcaatgc caattacccc gacacgacac ggcggacaca ttaccttgct
     1021 gaaaaaggga ttttgtttgt cggcgcgggc gtgtccggcg gggaagaagg ggcgcggcgc
     1081 gggccgtcca ttatgccggg cggggataag cgcgcttggg atgcggtcaa gccgattttt
     1141 caggcgattg ccgccaaaac gtcgcagggc gaaccgtgtt gcgactgggt cggcaaagac
     1201 ggtgcggggc attttgtcaa aatggtgcat aacggcatcg aatatggcga tatgcagttg
     1261 atttgcgaag cgtaccagtt catgaaagac ggcttggggc tgtcttacga cgaaatgtac
     1321 cgcgtgtttg ccgagtggaa caaaaccgag ctggattctt atctgattga aatcacagcg
     1381 gcaattttgg gctataagga cgaaggcggc gaaccgctgg tcgagaaaat cctcgatacg
     1441 gcagggcaaa aaggtacagg caaatggacg ggcatcaatg ctttggattt gggtattccg
     1501 ctgacgctga tttccgaagc cgtattcgcc cgctgcgttt cgtcgttcaa agaacagcgc
     1561 gtgcagaccg gcaaactgtt cgcccgaacc gttacgcccg tcgaaggcgg caaacaagaa
     1621 tgggtcgagg cgttgagaca ggccctgctt gcatctaaaa tcatttccta cgcacaaggc
     1681 tttatgctga tccgcgaagc gggcgaaagc tacggctggg atttggacta cggcaacact
     1741 gcgctgctgt ggcgcgaggg gtgcatcatt cgcagcgcat tcttaagcaa tatccgcgac
     1801 gcgtatgaaa acaatcccga tttggtgttc ttgggtgcgg atccgtattt caaaaatatt
     1861 ttggaaaact gcctgccggc gtggcgcaag gtggttgcca aggcggtcga atgcggcatc
     1921 cctatgccct gcatggcttc ggcgattacg ttcttggacg gctacacgac cgaacgtctg
     1981 cctgccaacc tcttgcaggc acagcgcgac tacttcggcg cgcacactta cgagcgcacc
     2041 gacaaaccgc gcggtgagtt tttccatacc aactggacgg gcaagggcgg agataccgct
     2101 tcgacaacct acgatatttg attccgttgc cgaaatgccg tctgaaccgt tcagacggca
     2161 tttgttttgc aggaaataga aagatgttcc aatggcttta tgatgtattg tggctgcttg
     2221 cgccgatatg gatacggcgt tatttggaca aacgctccgg aagtgccccg gcatatcggg
     2281 cgcatcggga cgagcgtttc ggcaagccgt atccgaatcc cgttaccggc gcggtttgga
     2341 tacacgccgt ttcggtcgga gaaacgcgtg ccgcccagtc cttgatacgc gagttgcggc
     2401 ggcgttttcc cgatgcgccg ctgctgatga cgcagatgac cccgacgggg cgggaaaccg
     2461 cgcaagttct gtttcccgat gcgcaatgcc gctatcttcc gtatgacaaa aaaacgtggg
     2521 tacggcagtt tttgcgcgaa caccgcccga tgttcggcat tttgatggaa accgaaatct
     2581 ggcccaacct gatgagggaa tgccggcgcg cgggtgtgcc gctgtttttg gcgaatgcgc
     2641 ggctgtcgga aaaatcgttg aacggttatc tgaaagtccg ccgcctgatc cgtcctgccg
     2701 ccgcttcgct gacggggtgt ctggcgcaga cagaggcgga tgcggcgcgg ttggcgaaat
     2761 tgggcgcggc atccgtgcag gtgtgcggca ataccaaata cgacatcata ccgtcggaac
     2821 agatgaaaac gctggcgggg cagtttgaaa aacgcatcgg aggccggccg gttgccgtgt
     2881 gcggcagcac gcgcgtttat cggggtgaag acgaggcgga aaaactgctg gcggcgtggc
     2941 aacaatatcg cggcgatgcg ctgctggtcg tcgtgccgcg ccatcccgag cattttcaga
     3001 cggtatttga aacggcaaaa cgcttcgggt ttaaggttca gcggcgcagc gacggtttgc
     3061 cggtcgaacc tgatacgcag gtgtggatag gcgacagtat gggcgagctg tatgcgtatt
     3121 acctgtgcgc cgatgtcgct tttgtcggcg gcagtctggt cgattcgggt tgtcagaaca
     3181 tcatcgaacc gctttcctgc ggcgttccga cgatattcgg cttttcaacc tacaattttt
     3241 ccgaagcctg ccgacacgcc ttggcatcgg gtgcggcggt tcaagtcgaa tcggcggatg
     3301 cgtggcggga agccgttgaa aaaaccttat cgtccgaggg gggggggatg cagatgcagg
     3361 cgcgcgtgga cggctttatc gcacaacatc gcggagcggg cgcgagaatc gccgaggcgg
     3421 tgcgggaagc ggtatgcgga tatcgggggc gatagtgata caatccgtat ccgagttttc
     3481 cgtttggagc atcgtatgag tatttatgcc gtcgcgcaca tcgttcatct gtattgcgct
     3541 attgcctttg tcggcggcgt gttttttgaa gtgctggttt tgtccgtcct gcatacggga
     3601 cgggtgtcgc gcgaggcgcg gcgcgaagtg gaaaaggcaa tgtcttaccg cgccgtcagg
     3661 gtgatgccgt ttgtggtcgg actgctgttc gccagcggca tcgtgatggc ggcaaaccgc
     3721 tatctttcta tattgggcga accgtttgcc acttccttcg gtacgatgct gacgctgaaa
     3781 atcctgttgg cgttcagcgt attggcgcac ttcgccatcg ccgtcgtcaa aatggcgcgt
     3841 tccacactga cggtcggttg gtcgaaatac atacacgccg tcgtctttac ccatatgctg
     3901 ctgattgtct ttttggcaaa agcgatgttt tatatcagct ggtaacacaa ttacatttca
     3961 aatcaggaga ttgaaccatg acagaatatc ccggcatcgg gtcgccgttg ttttacggcg
     4021 ttttttttgc ggcagtgctg gtcatgattg ccttggatat gttttcgctg aagaaaaacg
     4081 gcagccataa agtcggcgtc aaagaagcct tggcatggag cggcttgtgg gttgccgtat
     4141 cctgcctgtt cgcgggctgg ctgtattttg aactcgccgg caatcccggc tacggcgcgg
     4201 cagtcgcaaa agaaaaagta ttggaattct ttaccggcta cattttggaa aaatcgttgg
     4261 cagtcgacaa tattttcgtg ttcctgatga tattcggcta cttcaaagtc gcaccacagt
     4321 ttcagcaccg cgtgctgctg tacggcgtat tgggcgcatt ggtattgcgc accgtcatga
     4381 ttttcgtcgg cgcggcactg gttcagcagt ttgagtggat tctgtatctg tttggcgcgt
     4441 tcctgctcta tactggtata cacatgatga agcccgaagg cgatgagaaa gaggatttgg
     4501 caaacagcag gctgctgaat accgtcaaga aagtcgttcc ggtcggcacg gaatttcacg
     4561 gcgagaaatt ttttaccgtc gaaaacggca aaaaaatcgc cacgccgctg tttttggtgc
     4621 tggtcatgat tgaattgagc gatgtcgtgt ttgccgtgga cagcatcccc gccgtctttg
     4681 ccgttaccac cgatccgttt atcgtgctga cttccaatat tttcgcaatt ctgggcttgc
     4741 gggcgatgta tttcctgctg gcggatgtgg cggaacgctt tatcttcctg aaatatggat
     4801 tggcattcgt gttgggtttt atcggtgtga aaatgctggt aatgcattgg gtgcatatcc
     4861 cgatttccgt ttcgctgtcg gtcgtgttcg gcgcattggg tgcatcggtg ttgacttctt
     4921 tggtttatac gaaaaaaaca ggccgataag cggatataac gcccgtccgc ccgcaagggg
     4981 cttgacaggg tttcggccgg tctgtaaaat tccaaactta tcgggtcgtt agctcagccg
     5041 gtagagcagc ggacttttaa tccgttggtc gagcgttcga atcgctcacg acccaccaga
     5101 taaagcaaaa aagctgagtt ttcaaaactc ggcttttttg tcttccgaca atccttgttc
     5161 ggaaagcgcg gcatttcgtc aaattctttg gtgcggcttc cgtatttttc ccgaaaatcc
     5221 cttataattc cgcctctgca accttcatcg ggtgatgctt tatgcgttgt cccgagaatt
     5281 gaaatattat agtggattaa caaaaaccag tacggtattg cctcgcctta gctcaaagag
     5341 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ctgtactatc tgtactgtct
     5401 gcggcttcgc cgccttgtcc cgatttttgt taattcacta tacaagtacc caccaggaaa
     5461 gaacaaacgt ggacaaactg aaaatctccg caaacggccc gctcaacggg gaaataacgg
     5521 tctcgggcgc gaaaaacgcg gcattgccgc tgatgtgcgc gggtttgctg acatcgggta
     5581 cgttgcgcct gaaaaacgtc cctatgctgg cagatgtgaa aaccacgcaa aagctgcttc
     5641 aggggatggg cgcgcgcgtc ctgaccgaca atatcagcga atttgaaatc aacggcggta
     5701 cggtaaacaa tacctgcgcc ccttacgagt tggtccgaac gatgcgcgct tcgattttgg
     5761 tgctgggccc gacgctggcg cgtttcggcg aggcgcaagt cagcctgccg ggcggctgcg
     5821 ccatcggttc gcgccccgtc gatcagcatt tgaaaggctt ggaagcgatg ggtgctgaga
     5881 ttgttatcga acacggttac gtcaaagcca aaggcaaact caaaggtacg cgcgtggcga
     5941 tggatgtcgt taccgtcggc ggcacggaaa acctgctgat ggcggcgacg ctggcggaag
     6001 gtacgacggt tttggaaaac tgcgccattg agcctgaagt ggtcgatttg gcggaatgcc
     6061 tggtcaaaat gggcgcgaaa atcagcggca tcggtacgtc cacaatgatt gtggaagggg
     6121 tggacgagct gcaaggctgc gaacacagcg tcgtccccga ccggatcgag gcggggacgt
     6181 tcctgtgcgc ggtggcgata accggtggca gggtggtttt gcggaatgcc gcgccgaaaa
     6241 cgatggaagt ggtgttggac aaactggttg aggcaggtgc ggtgattgag gcgggcgacg
     6301 attggatcgc catcgatatg cggcagcgtc cgaaggcggt ggacatccgc acggtcgtcc
     6361 accccggctt ccccacggat atgcaggcgc agtttatggc attgaatgcc gtggcggagg
     6421 gaagctgccg cgtggtggaa acgatttttg aaaaccgctt tatgcacgtc cccgagttga
     6481 accggatggg ggcgaacatc acaaccgagg gcaatacggc atttgtgcag ggtgtggaac
     6541 agctttccgg cgcagtcgtc aaggcgacgg atttgcgtgc gtccgccagc ctcgttatcg
     6601 ccggtttggc ggcgcgaggc gaaaccgtgg tcgaacagat ttaccacttg gatcgcggtt
     6661 atgaaaatat tgaaaaaaaa ctcggcagcg tcggcgcgaa aatcgagcgc gtatccggct
     6721 gatcgggttg cgatgccgtc tgaagccttc agacggcatc aattgtttcc gtatcgcaaa
     6781 cactgattga aaaggactga ttatggcatt tttaaaactg accgaacaaa acgtgcaggg
     6841 caaaaccgtc ctcatccgcg ccgatatgaa cgtaccgttc aaagacggca aaatcagcga
     6901 cgacacccgt atccgcgcct cgctcgcgtc cattaaatac tgcgtggaca acggtgcgtc
     6961 cgttatcgtg atgacccatc tcggccgccc gaccgaaggc gagttccacc ccgaagacga
     7021 tgtcgccccc gttgccgcgc acttgggcag cctgttgggc aaagacgtga aagtattgaa
     7081 cgactggcgt gaaaacaaac ccgccctgaa cgcgggcgat gtcgtcatgc tgcaaaacgt
     7141 gcgcatcaac aaaggcgaga agaaaaacga tttggaactg ggtaaagcct acgcttcttt
     7201 gtgcgacgtg ttcgtcaatg acgcgttcgg aaccgcccac cgcgcccaag cctcgaccga
     7261 agccgtcgcc caagccgcgc ccgttgcctg cgccggcgta ttgatggcgg gcgaactcga
     7321 cgctttgggc aaagccttga aacagcccgc gcgcccgatg gttgccatcg ttgccggcag
     7381 caaagtgtcc accaagctga ccatcctcga atcgctggcg gacaaagtcg accaactcat
     7441 cgtcggcggc ggtatcgcaa acaccttcct gttggcggaa ggcaaagcca tcggcaaatc
     7501 tttggcggaa cacgatttgg tggaagaatc caaaaaaatt atggcgaaaa tggcggcaaa
     7561 aggcggttct gtgccgctgc cgaccgatgt cgtcgttgcc aaagcctttg ccgccgatgc
     7621 ggaagctgtg gtgaaagaca ttgccgccga tgcggaagct gtggtgaaag acattgccga
     7681 cgttgccgaa gacgagatga ttttggacat cggtccgaaa tccgctgccg cgcttgccga
     7741 tttgcttaaa gccgccggta cggtcgtttg gaacgggccg gtcggcgtgt tcgagtttga
     7801 ccaattcgca ggcggtacga aagcccttgc cgaagccatt gcccaaagca aagcgttctc
     7861 gattgcgggc ggcggcgaca cgctggcggc gattgccaaa ttcggcgtta ccgaacaaat
     7921 cggctacatc tccaccggcg gcggcgcgtt ccttgagttc ttggaaggca aagagctgcc
     7981 tgccgtagcc gctttggaaa aacgcggcgc gtaagcggtt tgatataaaa aaaatgccgt
     8041 ctgaaacgaa atggtttcag acggcatttt tgtgtggcga ttattgtgct ttggctgccc
     8101 attccgccgg agtggcggca accgaaatgg aaagtgcgtg cagttcgtta ctttccagtt
     8161 gggctttgag tccgtcttta atcaggcggt ggcgcgcgag gcgtgccttg ccttcaaatt
     8221 ctgatgaaac gatgacggcg aaaaaatggt gtccgtcgcc ttctacttcg atatgttcgc
     8281 attttgccac gccttcaatc atggctttta cttgttccga ggtcagcatg gtaggttcct
     8341 tgttggaaaa atgcgccatt atactggcaa attgaaatgc cgtctgaaaa cgggttcgga
     8401 cggcattttt gacggtgttt tattttttgg ctttgaagcc gagcaggtgc tgggtggtgg
     8461 caagccacgc gccgaatacg cccaacgcga tgacgaagcc gaacaccagc ccgagttcgc
     8521 cagcgtagaa gaaccgccag ccgatattaa gtccgtaggg tttgaaaatg gcatcgacca
     8581 atgggcgcac ggcagagagc agccaaccgc aaagccccaa gctgacggcg gcggaaagga
     8641 tgctctgcca catggcttga taaaggaatg ggcggcggat aaacgacgcg ggcgcgccca
     8701 agagtttggt gatttcgatt tcttctttgc ggctgaggat ttgcaggcgg atggtgttgt
     8761 gtgcgacaag gacgaacgcc atccccagcg tcagggaaag aaaccacaaa attttgcgga
     8821 tgaactcgtt gatttggtac agcgtttgca cccattcggt atccatagac gcggattcga
     8881 ccataggcag tttggtaatg tctcggtaga ttgcctgcat ttgggcgggc gtggttgccg
     8941 ggtcgggggt aacgataaag acatccggca gggggttgcc gtcaagcatg gaaatcagat
     9001 tttggtcgag attggactgt aattcttcca gaccgtcttc tttgccgatg aagcggatgt
     9061 tgtcgagccg tttgtcgcgc gccagcaggc tgcggacggt atcgctgtcg ctttgtgcgg
     9121 cggaggtttc catatagatt gtgatttgcg gcgactcgtt gagtttgccc aacacgcttt
     9181 gcccgctttg gatgcccaga tgcataaaca gcggcagggt catcgcgacg gcgagcatca
     9241 tgagggtaag cagtgtgccg aagggttggc gcaggagctg cttgagcgcg gtgcgcgcgg
     9301 attcgacgtg cagcgagagg tagtggatga tgctcatgcg agtcgtcctt tcgagaggcg
     9361 caggatgcgg tgtccgtagt ccgccatcag ggtttcgtca tgtgcggcaa cgatgacggt
     9421 agttcccgct tcgtggaagg ttttgaacaa ttccataata tcgagcgcgt aggcgcggtc
     9481 gaggttggcg gagggttcgt cggcaatcag caggccgggc tggtgaacga cggcgcgggc
     9541 gatgcacagg cgttgttgtt caccgccgga gagggttacg ggatcgtcca attctcgtcc
     9601 tttcaggccg actttttcga tggcgatgcg ggcacgctct tcggctttgc gcggcggata
     9661 gccgataatc cgaagcggca ggatgacgtt ttgcaggacg ttgcggtcgt agaggatttt
     9721 gtggtcttgg aacacgatgc cgatgtgttg gcgcataaag ccgatttggt tgtcggacaa
     9781 tgtgccgagg tcctgcccgt taaacaggat tttgcccctg ctcggcttgg taatgcccga
     9841 aatcagtttg aggatggtgg atttgcccga accggagtgt cccgcgataa atatcatttc
     9901 gcctttgttg atttggaagc tgacgttttt cagggcttca aaaccgccgg gataggtttt
     9961 ggaaacttgt tcgaaacgga tcataggagg tcctgtaagg aagggcggca tatcgtttcg
    10021 ggcggttgcg tccgcaggga tatgccgtct gaacaatagg taagacagtg ccgattataa
    10081 agattccgcc tgtttttaga tagtggataa gtttgcaccg gcaggcgggt ggaaaagaaa
    10141 aatgccatct gaagcggctt cagacggcat ccggcgttta acggcatttt gaatgggcga
    10201 gtttgacggc gtcggtcagg cttttttcgt ttacctcccc ggtaatggtc tgcctgtatc
    10261 cgcatttcgg tgcttcgacg acggtaaagg gcagtacgcc gacagtgttt ccgtaggttt
    10321 tcataaagtt tcggctgttc gccccggtgt aacgccaaat cgggtaggaa acaggagttt
    10381 gtttgaggaa gttgccgata ttgtcggatg tgtcgagcgc gatgccgacc atatcgacgc
    10441 tgcctttttt ctgcgctttg taccatttgg acatggcagg catctctttt cggcacgggc
    10501 cgcaccaagt cgcccaaagg ttgacgatgc gtacgggggc tttgagcgat tgcaggcttt
    10561 gcggggtgtt gtctttccat ccggccagtt cgtctgccga ggcggtatgt gcggcaaatg
    10621 tggcggcaag ggtgattgcg gcaagattca agtatttcat cgttttgtcc tttgtatgtc
    10681 gattcgggta ttgtaaaccg tttggtacgg ttgtaggcgg gatattgagt aaaaccctgt
    10741 gtaagccgat aataagggga ttgtcgagaa gcaaggaaat gtcatgcctg aaatcaaaat
    10801 tttccacaat ccgcgttgca gcaaatcgcg tgccgccttg tccctattgg aagaacgcgg
    10861 cattgctgcc gaagtggtca aatatttgga tacgccgccc gacttgtccg aattgaagga
    10921 tattttcaac aaattgggct tggcatcggc gcgcgggatg atgcgcgtga aagatgattt
    10981 gtataaggaa ttgggtttgg acaaccccaa tttggacaat gacgcgctgc tgcgtgccat
    11041 cgccgatcat cccgccctgt tggagcgtcc gattgttttg gcaaacggta aggcggctgt
    11101 cggcagaccg ctggaaaata tcgaagcggt attgtgaaca aacggctgtt ttgcagccgc
    11161 aacggcttgc gctattacct gttgggcggt ttctgtttga gtgtttttcc gcttttgctg
    11221 gtgtttgcct catccgtttg ggcggtttat cggacgggtg ggcaggtttt gccgccgtat
    11281 gtccgggcgg atgccgcgct ggtgttgggc gccgccgcgt gggacaaacg cccttctcct
    11341 gttttccgcg aacgcatcaa ccatgccatc gcactttatc agagccgcag ggtaggcaaa
    11401 atcgtgttta cgggaggccg gaccaaaaaa ggctatatga ccgaagccga ggtcgggcgg
    11461 cggtacgcac tcaaacaggg ggttccggcg cgcaacatcc tgtttgaaaa cacttcgcgc
    11521 aacacttatg aaaacctgaa caacatccgt ccggttttgc gtgccaacgg tattgccagc
    11581 gtggtgattg tcagcgatcc ctaccatttg gcgcgggcgg cggaaatggc ggaggatttg
    11641 ggtgtccgcg tttatatgtc tgccacgccg accacgcggt ttgatgcggg aaataagaaa
    11701 aaaatcttta tgctgcaaga gggatatgcg ttatccctct accgtttgga aaaatggggg
    11761 cgggattttt tcaattagct gtttcattga actttttttt ggacgcaatc ccgaaggaat
    11821 tcggtcggcg gcgcgtgata cactgttccg cgtgccgaag cattgaaaac aaggaaaatg
    11881 ccgtctgaaa ctttatccgc aggcagggtt cagacggcat ttttgatgcc caagcacggt
    11941 aaaaccagta aaataaacgc tttcaaagat ttcggagcca tcatgaccgt caaaacccgt
    12001 ttcgccccca gccccaccgg ctacctgcac atcggcggcg tgcgcaccgc cttgttttca
    12061 tgggcgtttg cccgccatca taaaggcgag ttcctattac gtattgaaga caccgacttg
    12121 gcgcgttcta ccgccgaatc cgtcaacatc atcctcgacg gcatgaaatg ggtcggtctc
    12181 aattacgaca acgccgacaa cgtcgtttac caaactcgcc gtttcgaccg ctacaaagag
    12241 gtcattgccg aacttttaga aaaaggccat gcctactact gctattgcag caaagaagag
    12301 ctggaagcca tgcgtgagaa agccgaaaaa gaaggctcgg cgacttacga ccgccgctgg
    12361 cgtccggaag taggtaaaac cctgcccgaa atcccgtccg acgtacaacc cgttgtccgt
    12421 ttcaaaacgc ctttggacgg tgtgaccaaa tggacagact tggtcaaagg cgaaatctcc
    12481 atccccaacg aagcactcga tgacctgatt atcgcccgcg ccgacggtac gccgacctac
    12541 aacttctgcg ttgtcgtaga cgactacgac atgggcgtta cccacgttat ccgcggcgac
    12601 gaccatgtga acaacacgcc gaaacaaatc aacatcttaa aagccatcga cgcgaacctg
    12661 cccgaatacg gccacctgcc gatgattctt aacgaacaag gcaaaaaaat ctccaaacgc
    12721 agcggcgata ccgttgccat taccgatttc ggcgcaatgg gcatcctgcc cgaagcgatg
    12781 ctcaactatc tggcacgctt gggctgggcg cacggcgacg atgaattctt cacaatggaa
    12841 caattcatcg aatggtttga tttgaaagac gtttccccgt ctccaagccg tatggacttg
    12901 aaaaaactct actggatcaa cggcgaacac atcaaaatca cacccaacgg caaactcgcc
    12961 gaactcgtca aaccccgcct tgcgttgcgc gatattcatg aaaccgaaaa acctgctttg
    13021 gaagatgtgt tggaactggt caaagaccgc ccccaagact tgaacacgct tgccgacgag
    13081 tgcttctact tctacgtcaa acaaacgcct gccgaagcag acgtgcagaa acactgggac
    13141 gatgaagccg ccgcccgtat gctgcgcttt gccgaacgcc tcgaagggct ggaagactgg
    13201 aatgccgaag ccatccacga cctcttcaaa cccttctgcg acgaagaagg cattaaaatg
    13261 ggcaaactcg gcatgcccct gcgccttgcc gtctgcggta cggcgaaaac ccccagcgtc
    13321 gatgctgtgt tggcattaat cggcaaagaa gaagtgttga aacgcatccg cgcttaagtt
    13381 atccacaggc gtgggcgatg ccgtctgatg tcggaatacg gcttcagaca gcatttaaat
    13441 ggaagttaaa aatgaaaaaa atattttatt ttctgatggt tgttttttct acaagcgtat
    13501 gggcagggga tgctgaagac aatctgctca gcatccaatc cggttaccgc gccttattgc
    13561 aaaagcaaaa caatctggac ggaaaaatca tcgggatgca gtcggatttg gaagatgcgc
    13621 gccggcgttt gcagacggct caggcagaca tcgcccgttt ggaagcggaa attcctgcag
    13681 caatggcgca aaaagcccgg caggctgaag atttgaggca aatcggagtg cgtttggacc
    13741 atgcttggaa tgcggtttac ggcgcagggg gaacgaaggc gtcggggaat tgaccgatga
    13801 attccctctt tgtggacaat actgttttca ttacacggct gaaagccggg catatcggca
    13861 ggttggttca ggcgttgttt gaggagtggc acggatttga accgtggtct tctgtggata
    13921 agattcatgc ctattacggc aggtgtttga aggatgacga actgccgctg gcatttgcgg
    13981 ctgtggatga ttccggaatc ctgttgggtt cggctgcggt caagcggcat gatatggaaa
    14041 gttttccacg gtatgaatat tggttggggg atgtctttgt tttacctgaa tatcgcggaa
    14101 aaggcattgg caggaggctg gtcgcccact gcataggcgc agcgcgttcg ctggggataa
    14161 agttcttgta tctttatacg cctgatgtgc aaatatttta tgaatcattc ggctgggtgg
    14221 ttgtcgggcg acatttccat aacggtgaat gggttacggt tatgcgtttg gatgtggata
    14281 aggtttaagc cgaatggacg gatgccgtct gagctgttcg gacggcattt tttatgtttg
    14341 gaaatttcct gtcagataat gtttatccac agatttttcc atatggtttg atgctgttcc
    14401 gacaacggaa attaagatac ggatttgcac aaatctttca gacggtacag ttctgacaat
    14461 gcttcgcgcg gggtcaggct gtcgggatcg agtttttcca aggctgctgc caatatacct
    14521 tcaaaatgtt tttcctctgc tttatccaca aagttgccca cattcggttc atctcctttt
    14581 tcagacggca tggtactgaa aatatccagt tggggacggt tcgcggcggc ttggttttcc
    14641 agtccgttca aatgcttttg ggcggatttc aatgcgcgta caggcaggcc ggcgagtttg
    14701 gcgacggcaa tgccgtagct tttaccggcg ggacccggtt ggatttggtg caggaaaacg
    14761 atgtcctgtc cctgttcgag cgcggaaagg tgcatattga cggcggcggt gtgggcttcg
    14821 ggcaggtagg tcagctcgaa atagtgggta gcaaacaggc tgaaggattt gtttttttgc
    14881 agcaggtgtt cggcaacggc gtgcgcgagg gcgaggccgt cgaaagtgga agtaccacgt
    14941 ccgacttcgt ccattaaaac aaggctttgt tcggtggcgt gatgcaggat gtaggcggtt
    15001 tcgctcattt cgaccatgaa agtggagcgg ttggaggcga ggtcgtccga tgcgccgatg
    15061 cgggtgaaga tttgatcgat gggcccgatt gtggcggcat cggcaggcac aaaacagccg
    15121 gtgtgtgcca ataaaacaat cagcgcgact tggcgcatgt aggtggattt gccgcccata
    15181 ttggggccgg tgagcagcat gaggcggtgt ttgtggtcaa ggtcggtgtg gttggcggtg
    15241 aagtggcgta cctgctgttc gacaacggga tggcggccgt tttcgatgtg gataaccgga
    15301 tagtcggcaa actcggggcg gacgaagttc cgctcttttg ccaaggctga aaatgtggac
    15361 aacacgtcca gcgcggcggc ggctttggcg gctttttgaa gctgcggcaa tgccgtctga
    15421 aggtttttca atacgccgtc aaagagttgt ttttctaagg cgagggcttg ctcttgagca
    15481 gtcagcactt tgtcttcaaa ggctttcagt tccggcgtga tgaagcgttc ggcgttttta
    15541 agggtttgcc ggcgttggta gtcggcaggt gcttgttcgg cttgggtttt ggacaattca
    15601 atgtaaaagc cgtgaacgcg gttgaactcg actttaagtg tggataaacc ggtacgttcg
    15661 cgttccttgg cttccaaatc cagcaaaaat tcgtcgccat ggttttgaat gcggcgcaat
    15721 tcgtccagtt cgggatgaaa accgtggttg atgacattgc cgtctttcag ccagacggaa
    15781 ggttcgggca aaatggcttg gcggagctgt tcggctgtgg ataggttttc cgggaaaacg
    15841 gctttgaggg ttcctaagag actgctgcac tcggcggaca attcgatttc ggacagggca
    15901 aacaggctgt cgcgcagggc ggcgaggtcg cgcgggcggg cgttacccac ggcaatacgg
    15961 gcggcgatgc gttcgatgtc ggcaatgctt ttcagacggc actggagggg tttgtattgg
    16021 ctttccagcg cggcaacggc ttcttggcgc gctcggatgt gggcgcggtt gcgtaaaggg
    16081 tggtgcagcc agagagccaa gaggcggctg cccatatggg tagcgcaaag gtcgagcgtg
    16141 gacatcaggg tcggcgattt tttgccggag agggtttgcg tgatttcgag attgcggcgc
    16201 gtggcggcat ccataccgat atattggctg tcggtttcga gcgacaggcc gtccaaatgt
    16261 tgcggcatca ggttttgcgt cagacggata tagttcaaca gtgcacctgc cgcgccaatc
    16321 gcaacggcgt gttctttgcc gtccaaaccg aagccgcgca aatcctggca gccgaaatat
    16381 tccgtcagca gtttttcccc cgcgtcggcg gcaaactgcc acgcgttcag gcgcgtaaca
    16441 cccgatgccg tctgaagttg cggtgcgttt ttactgtcag gcaacagaat ttccgccgcc
    16501 tgcaggcgcg ccagttcgtc gtccaattta tccacagttg tcagcttggt tttgaattcg
    16561 ccgctttgca gcgatgccca cgccaaaccg atgtattttt tgtcggggga cacggcaacg
    16621 atgcggttgg tttccttgtc ttccagcaat gcggaatcgg tcagcgtgcc gggcgttacg
    16681 atgcgcacga ctttgcgctc cacaggccct ttgcccgcgc cgacttcgcc gacctgttcg
    16741 caaatcgcca cgcttttgcc caacttgacc aggcgcgcca gatattgttc ggcggcgtga
    16801 aacggcacgc ctgccatttt gaccggctcg ccatccacct gtccgcgcgt ggtcagggta
    16861 atatccaaaa gttttgccgc ttctaccgca tcgtcgaaaa acatctcgta aaaatcgccc
    16921 atacggtaaa acaccagttt gtcggtatgt tgcgctttga tgccgaggta ttgctgcatc
    16981 attggggaaa cggcggattt ggacatgggg ctgccttatc attaggaaaa cggtattgta
    17041 gcaaaaggtt ttgtcttcga cggaatgaga ctgaccgtaa gcctgtcaat aaggttgtgg
    17101 agacaactag gaaaacagca aagttatcca cagaaatatt attaagtcaa aaaacatcca
    17161 cgcttatctg tcttatacag caagtcttaa actcaaactt atttgacaaa aaggtaatga
    17221 aaatattgga tatttgctac ttacgcacaa gcaggttggc aatcatcaaa atcatcatta
    17281 aaattttaaa ttcaaatatt gatggtaaaa gttttggaaa tctgtgcaaa cagtaaaatt
    17341 catctatatc aaataagcgg caagtagtta gatgtaatag tggaatgtaa ctttatttca
    17401 gaatacaata caggtattga aatttcgttt cacagcttgg agtatataca tgagcattaa
    17461 agtagcgatt aacggtttcg gccgcatcgg ccgcctcgca ttgcgtcaaa ttgaaaaagc
    17521 ccacgacatc gaagtcgtcg ccgtcaacga cctgacccct gccgaaatgc tgctgcacct
    17581 tttcaaatac gacagcacac aaggccgttt ccaaggtact gccgaattaa aagacgatgc
    17641 catcgtggta aacggcaaag agatcaaagt ttttgccaat ccgaatcccg aagaattgcc
    17701 ttggggcgag ttgggtgtgg atgtcgtcct cgaatgtacc ggcttcttta ccaataaaac
    17761 caaagccgaa gcacacatca gggccggcgc gcgcaaagtc gtgatttccg cacccggcgg
    17821 caatgatgtg aaaaccgtcg tatatggcgt aaaccaagac attttggacg gcagcgaaac
    17881 cgttatctcc gccgcttcct gcaccaccaa ctgccttgcc ccgatggcgg cagtcctgca
    17941 aaaagagttc ggcgtagtcg aaggtctgat gaccaccatc cacgcctaca ccggcgacca
    18001 aaacaccctt gacgcgccgc accgcaaagg cgatttgcgc cgcgcccgag ccgccgcgct
    18061 caacatcgtg cccaacagca ccggtgccgc caaggccatc ggtctggtta tccccgaatt
    18121 gaacggcaaa ctcgacggct ccgcccaacg cgtccctgtt gccagcggct cgctgaccga
    18181 attggtttcc atcctcgaac gccctgtaac caaagaagaa atcaacgccg cgatgaaagc
    18241 cgccgccagc gaatcttacg gctacaacga agatcaaatc gtttcttccg acgttgtcgg
    18301 catcgaatac ggctcgctct tcgatgcgac ccaaacccgc gtgatgaccg tgggcggcaa
    18361 acaattggtg aaaaccgttg cctggtacga caacgaaatg tcctacacct gccaactcgt
    18421 ccgcactttg gaatacttcg caggcaaaat ctaaggcttg aacaaacctg tggataagta
    18481 aatgccgtct gaacacattg cgttcagacg gcattgttta tctgtatagt ggattaaatt
    18541 taaaccagta cggcgttatc tcgccttgcc gtactatttg tactgtctgc ggcttcgttg
    18601 ccttgtcctg atttaaattt aatccgctat aacttgatat tgcggcttat ggcgaacaaa
    18661 caacagccgt ttgttcaact gctatgcggc aaaaccggat ttttgcccga tttaaagcgg
    18721 ggttggggaa agatcaataa cggttgcacg gttttcctcg atttgaaatc tgacttcgta
    18781 atctgcaata ttcatcacat aaatccgttc gggaatattc tgataggcgg ggcgcggatc
    18841 ttgggcaatg ctttggctga taaggttttt ggtgtttgca gataaatttt ccgcgccgat
    18901 gttttcctgc caaacgactt ccaactctac gggtttgccg ctgacgaaac cggatgcggc
    18961 atcgggtttg gattcgacaa aggggatata aggtttgatg tccacaatcg gtgtgccgtc
    19021 cagcaggtct gcgccgctgc aatagaggcg gacgggtttg ccggtttcga tgcgttcgag
    19081 tttcaggagc gagagtccga gatggttggg gcggtggggg ctgcgcgtgg cgaacacgcc
    19141 cattttttgt ttgccgccga gccgtggcgg gcgcaccatt tgcgcccagc cttcatccaa
    19201 tacgccgtga aaaataaaac ttatccacac ataatcgaaa tcttccagcc cgcgcacgct
    19261 gtctgcggtg aatttgggat tcagctcgat gcaggctttt gcggcggaga ccaaaccggg
    19321 ctggcgggcg atgccgaatt tctgtttgta gggcgagcgg gcggtgccga tgggggtaat
    19381 ggtataagtc ataaggttgt ggataagttt tttggaaaaa ccttatctta tcacaatcgg
    19441 ttttaccgct tttgggcagt aatttgttat aatacagggc attttaatcg cgttgaaata
    19501 atgagatgat tgtttccatt gatgttgatg cacaaaaaac gtttacgcca ctgtgtcctg
    19561 acgaactgcc cgtgaacgag gggcatttga ttgtcgagga gttgaatgcg caagccgctt
    19621 tggcggattt gcgcgtgatg acgaaagatg cgcatcatat ggtagcgaaa tggcttgtgg
    19681 ataaccctgt tgatatgttg aagccgacag gtttgcctga tgcggatttg acttgggtgg
    19741 ctcatgcgat ggtcggtacg cgcggctatg aattattgga cgggctgcct tctgctaaag
    19801 aatacgatta ttgcgtttgg aaaggtgttg atcctgaatt gcatccttac ggcgcgtgtt
    19861 ttcacgatat tgaggaaaaa ctaagcacag ggctgattga atggctgcgt tgtcaaaata
    19921 cggatacggt tattatcggt gggttagcta cggattattg tgttaaaaca acggttttgc
    19981 agttactcaa aggcggtcgt tggcaggttg tcgtcaatga agcggcttgt cggggtattg
    20041 cgccggaaac catcgaaaca gcatggcagg aaatgcattc tgctggggca atcatcttaa
    20101 aaaacgctga tgaaataaat aaatatatta ataatcaata agttaaaatt gtttcacgtg
    20161 aaaccccctt cctaaatatg aaaattttgc ttgtccgctt gtctagtatg ggcgatttaa
    20221 ttcacacttt gcccgcaatc gaagatttgg cgcgacaatg tcctgatgta gaactgcatt
    20281 ggctatgtga ggctggattt gcagatattg cgcgcctgca tccgtttgta aaaaaaatcc
    20341 atgtgatgaa atggcggcaa tggcgcaaac atctctttcg ggctgaaact tggcgggaaa
    20401 tgggtcatct gaaacagact ttgcggcagg aagtatttga tttcgtattg gacagtcaag
    20461 gtctgattaa aagcgcgtgt ttcgctaaaa tggcaaaatc cccgatttgt ggtttggata
    20521 aaaacagtgc gcgcgaggga tgggctgctt tggcgtatgt aaaaacatac gctgtaccga
    20581 agggaaaaaa tgccgtttgg cgcaaccgtg aactgtttgc ccaagtattt gggtatgtaa
    20641 tgccggaaac gcaggtattt ggcttgactg ttcctgaagc aggccgtctg aaaaatttag
    20701 cgcagccgta ttatgcggct ttgcatgcga ccagtcggga cagtaagtta tggcctgtgg
    20761 aaaactggcg gtcgttgctg caaaagttga atgaagaaca gcaatgcaat atttacctgc
    20821 cttggggaaa tgaagatgaa aaaacgcgtg ccaaacaaat tgcagatggg ctgccgtttg
    20881 ctattgtgtg cgacaaaata aatttattgc aggcagcgta tctgctgaaa tacgcggtcg
    20941 gaattgtcgg cgtggatacc ggtttgctgc atttggcaaa tgccttggaa aaacctgtgg
    21001 tcggcattta taccgatacc gatccgatta aaacaggcgt tcaggtttcg ccaattgcaa
    21061 aaaatttggg caatatcggg cagattccga ccgcagattt ggtttatcaa gcgctgatgg
    21121 attgtgttgc agcagataaa ggctaaaggg tcgtctaaaa ctgatatggc ggcatgatat
    21181 tttccaattt attagataca ggaatgtata tccgctagtc tgagtaaatg ctttaaaaat
    21241 ggtataatcg gcaagcatta tccgaaagat ttttgaatga attcgttttt aaggaaaacg
    21301 gatttggttt tatagggaag aaggtttggt ttcgctggat ggtgaaagca gcgttctttt
    21361 taggagggtt caatgaaagc actggtcgca gtaaagcgcg tagtggacta caacgtcaaa
    21421 gttcgtgtaa aagccgatgg ttcggatgtg gatatcggca atgtcaaaat gtcgatgaat
    21481 ccgtttgacg aaatcgctgt ggaagaagct gtccgtttga aagaagccgg aaaagtaagc
    21541 gaaatcgtag cggtttcttt gggtgagaaa aaatgcgaag aaaccttgcg tacagctttg
    21601 gcgatgggtg ccgaccgtgc cattcatgtt gaaaccgata ctaaactgga gtctctggcc
    21661 gttgctaagt tgctgaaagc cgttgcggac aaagaaaatc cgcaaatttt ctttttgggc
    21721 aaacaagcga ttgatgatga tgccaatcaa gtggcgcaaa tgctggcagc tttgctgaat
    21781 gcggcgcaag gtacgttcgc gtccaaagta caaattgaag gcgacgaagt gcagattgtg
    21841 cgggaaatcg atggcggcga agaaaccatc gcattgaaac tgcctgctgt tatcagcgcg
    21901 gatttgcgtt tgaacgagcc gcgctttgtc aaactcccca atattatggc ggcaaagaaa
    21961 aaacctttgg aaaaactgac tcctgacgat ttggttgccg acatttcacc tcgtttgaaa
    22021 acggtgaaat ttgccgaacc taaggcgcgt caggcaggcg taaaagtagc aagcgttgcc
    22081 gaattggttg aaaaattgaa aaacgaagcc aaagtgattt gaggaggctg aaatgagtgt
    22141 attgattatt gtcgaacacg acaacaaaca gttgaatcct accactttgc atgctgttac
    22201 cgctgccgcc aaactgggca aagtcgattt attggttgcc ggaaacggcg catctgctgt
    22261 agtggaattc gcgaagcaag tagcgggggt gaaaaaagtt ttggttgcag atgctgctca
    22321 ttatgccgaa ggtttggctg aagagttggc tccgctggtt gttaaattgg cggccgatta
    22381 ccgctatgtt gcagcaacgg caaccacatt cggtaaaaac cttttgcccc gcgtagcagc
    22441 cttattagac gtaccgcaaa tttctgattt gaccgaaatt gtggataaca cgacttttgt
    22501 gcgccccatt tatgcgggta atgcatttga aaccgtgcaa gctgattcag aaaaattggt
    22561 gctgaccttc cgtgcgacgg tttttgacgc agtggcggcg caaggtggta atgctgaggt
    22621 aattaatgtt gaagcaaccc ctgcccaaaa cctgagtcgt tttgttaacc gtcagctttc
    22681 ccattccgat cgtcctgaac tgactcaggc aaaagtgatt gtttccggtg gtcgtgcgtt
    22741 gggtagtgcg gaaaaattca atgaagtgct gacaccgttg gcggatgttt taggtgcggc
    22801 aatcggtgca tcccgtgcag cagttgatgc cgaatatgcg ccaaacgatg ctcaagtcgg
    22861 acaaaccggt aaagtggttg cgccgcaact ctattttgca atcggtattt caggtgcaat
    22921 tcaacacgtt gccggtatgc aagacagtaa ggtgattgtt gcaatcaata aagatgctga
    22981 tgcgccgatt ttcaatgtag ccgattacgg attggttggc gatttatttg aaatcgttcc
    23041 acaattaaca gaagtattga aaaattgatg tttcacgtga aactttcaga cgaccttgtt
    23101 attaatgagg tcgtctgaaa acatttcagc atgattacga cttataaaac cattacttcc
    23161 tcgacgcagg ctgagtttaa agataaaggc agccgtttta ttgcatttgc ctatccgatt
    23221 cggacattgg ctgatgtgaa aaaatacctc gatccgttaa aggaagagca tcataaagca
    23281 cgacactggt gctatgccta tcgtttgggt gtggatggtg tgcaatttcg tgccaacgat
    23341 gatggagagc catcaggaag tgctggacga ccaattttgg gacagattga ttcggtgggt
    23401 attaccgacg ttttggttgt ggtcgtccgc tatttcggtg gtactttgtt aggtgttcca
    23461 ggtctgatac atgcgtacaa agaggcgacg gctcaagcgt tggcagttgc agaagtagtt
    23521 gaaaagaata ttgaaaaaac tgtttggctg aaatgtgaat atccgttttt gaatgaagcg
    23581 atacgcattg ccaaacaata tcaggcggat atattggagc gggatttgca gttggattgc
    23641 cgattgacgg tcagtttgtc gttggtaaat tatgaggcct gtgttgcggc ttggaagaat
    23701 actcggcaga ttgaagtaaa tacggaaaag ccttttgaat gaaaggtcgt ctgaaatgct
    23761 ttgtgatggt atttcagacg acctcttcgc tgactaatct tcttgtgtat atttgtctaa
    23821 ggcatcgacg ctggagcgca aatataacca agccaattga tggaattcgc ccagcgcgtt
    23881 ccatagcgca tcttcactca taatgctgta ttcgccttcc gtactcaaat ccagttctac
    23941 cccgtcttca atagcctgat gacacgcctg atactcgtgg gtatagaaat tgtccatgtc
    24001 gcgaattaaa gtgacgacat gtttccaacg gcggatgtct tcttccagtt gcggtagtaa
    24061 atgacaccat tcgcggtatt tgctttggat ttcatcaata cgtttttgca tggttgcttg
    24121 tcctttttta atttatggat aagttgaata ggttgcagag tggaatgaag cggtcttttg
    24181 tttgattcta ctaaaatttc ttcagatggg ataaaagtcc ctttgtatcg agttttcgtt
    24241 aaaagtacat atttgtcaag gagatggtat gaaactgctg gttatcggta atggcggtcg
    24301 cgaacacgcg ctggcttgga aattggcgca gtcgcctaaa gtggaaacag tatttgttgc
    24361 gcctggtaat gccggtacag cgattgaacc caagttgcaa aacatcgact tgactgcgca
    24421 tcaggatttg attgaattct gtcgtaaaga aaatattgtt tttaccgtcg tcggccctga
    24481 agcgcctttg gcggcaggta ttgtggatga tttccgtgct gcaggactaa aaatattcgg
    24541 tccgacacaa tatgcggcgc agttggaaag ttccaaagat ttcgccaaag catttatggc
    24601 gaaatacaat attccgaccg cgcaatatca aacctttgaa aacgccgatg ccgcacatga
    24661 ttacgtcaat cagaaaggtg cgcctatcgt tatcaaagcc gatggtttgg cggcaggtaa
    24721 aggcgtgatt gtggcgatga ctttggatga agcgcatgct gcgattgatg atatgctgct
    24781 ggacaacaaa atgggcaatg ccggcgcgcg tgttgtaatt gaagatttcc tgcaaggcga
    24841 agaagcgagt tttatcgtca tggttgatgg caataatgta ctgcctatgg caaccagcca
    24901 agaccacaag cgtcttttag acggcgacaa aggtctgaat acgggcggta tgggtgcgta
    24961 cagccctgcg cctgtggtaa cgcctgttgt gtacgaacgc gcgatgaatg aaattatttt
    25021 gccgaccgta gcagggatga aagcggaggg gcatgagttc accggtttcc tgtacgcagg
    25081 tttgatgatt gatcaaagcg gtgcacccta tacgattgag tttaactgcc gtttcggcga
    25141 tcctgaaacc cagccgatta tgagccgact gaacagcgac ttatcggatt tggttgaagc
    25201 ggcaatagac ggcaaactcg atagcgtaac cgcggaatgg agcccgcaaa ctgccgtggg
    25261 cgtggtactg gcggcgcaaa attaccccga aacgcccaaa aaaggcgata ttatttccgg
    25321 tctggatgct gccaaccaaa tcggcaaagt tttccatgca ggtacaacgg caaacgagaa
    25381 aggtgacgta ttgaccaacg gcggacgcgt attgtgtgtt gtgggattgg gcgataatgt
    25441 ggcgcaagcg aaagctaagg catatggcgc gttggaaaaa atcagtttcg acgggatgca
    25501 ataccgtaaa gacattgccg ataaagcgat taaccgttaa ttagaaagag gatgatgccg
    25561 tctgaagcca agtatgacta atgtgcttca gacggtattt tatattatgt tatttccgag
    25621 aattatcgca gcatcagcgc aacgtaagtt aagcgtttat ttgaaaaaag gtggtttggt
    25681 cgcttatccg accgaatctt gttatggctt ggggtgtctt ccgacattag ccaaagcact
    25741 cggtaagttg gcgcatttga aaaaaagacc gcaacacaag ggtatgattg tcatcgggaa
    25801 tcaatttgaa caattgcagc ctttacttca aatgccgtct gaaaatcttc aggatatgct
    25861 tagaaaagaa tggcccgccc ctaaaacgtt cctattatcc gccaaatcct gtgtcttacc
    25921 cgagttacgc gggaaacagc gcagcaagtt ggcggtcagg gttcccgccc atgtaggtgc
    25981 gcgccgcttg tgtcaggctt tgcaaacgcc tttggtctcg acttcgtgca accgcgcagg
    26041 caaacgcgcg tgtcggacgg agagggaagt cagaaggcaa ttcggacgcg atgtgtggat
    26101 tgttggtggg agaatcggca ggcagaaatc gccgagtcag attattgacg gggaaacagg
    26161 taaacgtttg cgttagatat gggtacttga atgaaaactt tttttgcata cacaaagaaa
    26221 aaatttttcg gatagcattt ctctacatga ttgttattgc acagagataa aggtagaagg
    26281 acgcaacgtc tgctttaatt ttgaggacgg ttttgtcgtt ccggacaata atccgaacaa
    26341 tcaagcgggc aagcatctga aaacagattt ttcccgtgtt gttttaatcc ataaaaatga
    26401 aaacgccgaa gatgatta
//
LOCUS       DAWISC010000008        25547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWISC010000008 DAWISC010000000
VERSION     DAWISC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.008"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..347)
                     /locus_tag="WIW87_000244"
     CDS             complement(<1..347)
                     /locus_tag="WIW87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEZ5173463.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDG"
     gene            complement(344..1486)
                     /locus_tag="WIW87_000245"
     CDS             complement(344..1486)
                     /locus_tag="WIW87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5173464.1"
                     /translation="MGKSLTEIAEELKGNDKKVQLIYAFNGTGKTRLSREFKNLIAPT
                     SSEEPDGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDDFSEIAFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKLSK
                     PRLKFIITTHNVLFYNVLYNELKKLEKEKKSYLLLRNEDGSFDILEKQGDSNKSFSYH
                     LHLKGVIEKAIENQQVERFHFMLLRNLYEKTANFLGYKQRSDILPEDSRRNYFQRIIN
                     FTSHSTLSNEAFAEPTPQEQETVKLLLQHLLDNYNFFQDDEQRDKP"
     gene            complement(1544..2059)
                     /locus_tag="WIW87_000246"
     CDS             complement(1544..2059)
                     /locus_tag="WIW87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ5173465.1"
                     /translation="MFAGGDVPKDAFSEVETEEFCIPILSNGIGSKALYGWTNVAKIN
                     QPSLTISARGTIGWASFQNKPFFPIVRLLVLTPKIELNLKYAYYFMKSIESNYKVPES
                     GIPQLTKPMIKDISIPIPPLPEQEKIVAILDKFDTLTHSISEGLPYEIALRRKQYEYY
                     RGQLLSFPKAA"
     gene            complement(2301..2789)
                     /locus_tag="WIW87_000247"
     CDS             complement(2301..2789)
                     /locus_tag="WIW87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ5173466.1"
                     /translation="MDMQNKAKKLIEMIQTAPVEWKPLGEVAKVLRGKRLTKKELIEG
                     GKYPVFHGGLIPLGWFDQFNRRANQTMIINTGSIGEVIWSGVDFWSSDGTFVIQTPNY
                     LDDKFIFYFLKTREGYIKSQKRVGGVPTIDRLVVENISIPIPPLETQQKIVKILDKFT
                     EL"
     gene            complement(2955..3965)
                     /locus_tag="WIW87_000248"
     CDS             complement(2955..3965)
                     /locus_tag="WIW87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ5173467.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(3962..5506)
                     /locus_tag="WIW87_000249"
     CDS             complement(3962..5506)
                     /locus_tag="WIW87_000249"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ5173468.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFENHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLIEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(5580..6584)
                     /gene="rfaD"
                     /locus_tag="WIW87_000250"
     CDS             complement(5580..6584)
                     /gene="rfaD"
                     /locus_tag="WIW87_000250"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ5173469.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(6713..7744)
                     /locus_tag="WIW87_000251"
     CDS             complement(6713..7744)
                     /locus_tag="WIW87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ5173470.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMSQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(7746..8981)
                     /gene="dcm"
                     /locus_tag="WIW87_000252"
     CDS             complement(7746..8981)
                     /gene="dcm"
                     /locus_tag="WIW87_000252"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5173471.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(9018..9989)
                     /gene="hldA"
                     /locus_tag="WIW87_000253"
     CDS             complement(9018..9989)
                     /gene="hldA"
                     /locus_tag="WIW87_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ5173472.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(10044..10784)
                     /gene="pyrF"
                     /locus_tag="WIW87_000254"
     CDS             complement(10044..10784)
                     /gene="pyrF"
                     /locus_tag="WIW87_000254"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ5173473.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(11310..11957)
                     /gene="adk"
                     /locus_tag="WIW87_000255"
     CDS             complement(11310..11957)
                     /gene="adk"
                     /locus_tag="WIW87_000255"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ5173474.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            12173..13012
                     /gene="htpX"
                     /locus_tag="WIW87_000256"
     CDS             12173..13012
                     /gene="htpX"
                     /locus_tag="WIW87_000256"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ5173475.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(13313..13873)
                     /locus_tag="WIW87_000257"
     CDS             complement(13313..13873)
                     /locus_tag="WIW87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173476.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(13987..14379)
                     /locus_tag="WIW87_000258"
     CDS             complement(13987..14379)
                     /locus_tag="WIW87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5173477.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(14587..14979)
                     /locus_tag="WIW87_000259"
     CDS             complement(14587..14979)
                     /locus_tag="WIW87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5173478.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(15096..15506)
                     /locus_tag="WIW87_000260"
     CDS             complement(15096..15506)
                     /locus_tag="WIW87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5173479.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(15520..15957)
                     /locus_tag="WIW87_000261"
     CDS             complement(15520..15957)
                     /locus_tag="WIW87_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5173480.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(16369..17667)
                     /locus_tag="WIW87_000262"
     CDS             complement(16369..17667)
                     /locus_tag="WIW87_000262"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ5173481.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(17771..18922)
                     /locus_tag="WIW87_000263"
     CDS             complement(17771..18922)
                     /locus_tag="WIW87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ5173482.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            19257..20636
                     /gene="norM"
                     /locus_tag="WIW87_000264"
     CDS             19257..20636
                     /gene="norM"
                     /locus_tag="WIW87_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ5173483.1"
                     /translation="MLLDLNRFSFPVFLKEVRLLTTLALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYT
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            20680..21720
                     /gene="murB"
                     /locus_tag="WIW87_000265"
     CDS             20680..21720
                     /gene="murB"
                     /locus_tag="WIW87_000265"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ5173484.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELRDIVRLLEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            21732..22382
                     /locus_tag="WIW87_000266"
     CDS             21732..22382
                     /locus_tag="WIW87_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ5173485.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            22437..23234
                     /locus_tag="WIW87_000267"
     CDS             22437..23234
                     /locus_tag="WIW87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5173486.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            23285..23851
                     /locus_tag="WIW87_000268"
     CDS             23285..23851
                     /locus_tag="WIW87_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ5173487.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            23871..24761
                     /locus_tag="WIW87_000269"
     CDS             23871..24761
                     /locus_tag="WIW87_000269"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ5173488.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            24786..25544
                     /locus_tag="WIW87_000270"
     CDS             24786..25544
                     /locus_tag="WIW87_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ5173489.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
BASE COUNT         6102 a         6507 c         6119 g         6819 t
ORIGIN      
        1 ccgtcatcaa aagcgaaatc gtaaatatgg tcgtcgtgga ttttgcgggt tttatcggta
       61 atgttttcag acggcctgtc cagatattcc gtcaaaaacc gcgcccattc gccgtctgaa
      121 aacgccacat cgttcagccg ctgcaactgc gcgcgcaggt tttccagcag cctgctttgg
      181 ctgttcaaat ccttgcggta ttcgtaaccc tgattctgca aatcggcgat taactccgct
      241 tccaaccggt tttccgattg gtagctgccc gactgttcga ttttttcata ttggtcgagc
      301 acgatgaaat tcggcgtttc agcgatgggt ttggtttcga ggttcatggc ttatctcttt
      361 gttcatcatc ttgaaaaaaa ttatagttat ccagcaagtg ttgcaaaagc aatttgacag
      421 tttcttgttc ttgtggtgtt ggctcggcaa atgcctcatt agataatgta gaatgacttg
      481 taaagttaat aatacgttga aaatagtttc gtctgctgtc ttcgggcaaa atatcagacc
      541 tttgcttata gcctaaaaaa ttagctgttt tttcatacag gtttctcagc aacataaaat
      601 gaaaccgttc tacctgctga ttctcgatag ctttttcaat aactcctttt aagtgaaggt
      661 gatatgaaaa acttttattg gaatcacctt gtttttcaag aatatcaaaa ctaccatctt
      721 catttcttaa cagaagataa cttttctttt ccttttctaa tttttttagt tcattgtata
      781 gaacgttgta aaataaaaca ttatgtgtag taatgataaa ctttaacctc ggtttgctaa
      841 gctttatcaa atcagccaaa tcaaccgctt gctgaatcag atgattttca tccaaagagc
      901 tgacagggtc atcaataaaa atatattcca aatcgtcaaa ctgatctgtg gaacgtcctt
      961 cttcgctatc ttctggaata ttcaattcag cgatgacttg tctgattaat acatagaaaa
     1021 tgctccaaat aaaattactt tcttcacctt tggaaatttt gatattttca atctgctcat
     1081 cattaccacg agcaaaataa aatgcaattt ctgaaaaatc atcattaaaa tcaggagtca
     1141 acttatcatc tgtataatat tgaaagtttt taataacagc tccatccagt ccattatccc
     1201 taagcaacca gtcggtaaaa ctattctttt gaatttttaa tcttggagct tcgttcgcta
     1261 acaaatcatt gtcccaaaag aataaatcct cagtaaatgc attatagtag agaaattttc
     1321 ttcttgttgg ctctccgtct ggctcttctg aactggttgg agcaattaaa ttcttaaact
     1381 ctctggacaa acgtgttttc cctgttccgt taaaagcata gattagctgg acttttttat
     1441 cgtttccttt tagttcctca gcaatttcgg ttaaactttt tcccatgata tttccttatc
     1501 aatatttttc agacggcatg atttaaagac cagctatgac ttttcaggca gcctttggga
     1561 agctcaacaa ctgcccgcgg taatattcgt attgtttccg gcgcagggca atttcgtacg
     1621 gtaggccctc gctgatggag tgggtcaggg tgtcgaattt gtcgaggatg gcgacgattt
     1681 tttcctgttc ggggagtgga gggatgggga ttgaaatatc ttttatcatt ggtttcgtta
     1741 gctgtggaat accgctttca ggaactttat aatttgattc aatacttttc ataaagtagt
     1801 aggcatattt taggtttaat tcaatttttg gtgttaacac taacaggcgt actattggga
     1861 aaaaaggttt attctgaaag ctagcccaac ctatagttcc tctagctgat atagttaagc
     1921 taggttgatt gattttagca acattagtcc agccatatag tgccttactt cctataccat
     1981 tagataaaat ggggatacaa aattcttccg tttccacttc agagaaagcg tcttttggta
     2041 cgtcgcctcc agcaaaaata ttaaatacct cccccaacgt cttccaaacc acatctttca
     2101 gacggccttt atagccatca gctatccccc ccgatttgat tgttaaaatc taaaagaaag
     2161 tcgcggtaat accggtattg gcgtttgcgc agggttaatt ccgcttccag cgtagcttac
     2221 agcgtagctt acagcgtagc ttacagcgta gcttacagcg tagcttacag cgtagcttac
     2281 agcgtagctt acagcgtagc ttacagctct gtgaatttgt caagtatttt tacaattttt
     2341 tgttgggttt ccaggggtgg gatggggatc gaaatatttt caactactaa tctatcaata
     2401 gtaggaactc caccaactct cttttgggat tttatatatc cttctcttgt ttttaaaaag
     2461 tagaatataa acttatcatc aagatagttt ggtgtttgaa tcacaaaagt accatcagat
     2521 gaccagaaat ctacgccact ccatataact tcaccaatac ttcccgtatt aataatcatc
     2581 gtttgattag ctctacgatt aaactggtca aaccaaccaa gaggaatcaa tccgccatga
     2641 aaaaccggat atttcccacc ttcaattagc tctttctttg tcaaacgttt tcctcttaat
     2701 actttcgcca cttcccccaa cggcttccac tccaccggtg ccgtctgaat catctcaatc
     2761 aattttttcg ctttgttttg catatccatg gttgttttcc gtttctttcc cgttgcggcg
     2821 ggatgtgggg cagttgcggg gtgcttcggc tcactctgtc cgtttgccgt tatttctgcg
     2881 ggctgaagtc cgtcctgctt tcaggctgcc tgcaatggtg tgcaggctgc tttttcaggt
     2941 agcctgaatg gtagttattt tgtgcggtct tttttctgtt tgatctgttg cacggctgtt
     3001 ttttccaacg ctttaacgct ttccagatag gcttgctcca ccggcgacag gatgcgcgct
     3061 tcataggcgc ggtattcgcg ttcggctttc tgctctgcct gtttgcggct gatgctgcct
     3121 gcaccctgta atgtgccttg tccgtacagc ccggaaaatt tgtccaattc ggctatccag
     3181 tcgcgcatat acatggggct ttgctcctgc gctttgattt ccgctaggtc gaagaaggcg
     3241 gaaaccagac ggttcaggcg gaacagttcg tcttcggtca gatagttttt ggcgattttg
     3301 gcttcattca gcgtggggat tgcgccttga aaggtggtca gccccataaa gtctttgctg
     3361 ctgtcggcac ggctgtatat cagctcagct gcggtttgcc ggctggcggc atagtgcagt
     3421 ttgttttgaa cggcggcaaa aaaggtttgg ctttcgctgc ttttggggtt gtagtcttgg
     3481 ctggtggcat ataaatcaag cacttgccgg tatagggctt tttcgctgct gcggatgtcg
     3541 cgaatgcggt tgagcagttc tttccaatag tcgccgccgc ctgtgccttt caggcgttcg
     3601 tcgtctatgg caaagccttt ggtcagatat tcgtccagcc gttcggttgc ccattggcgg
     3661 aattggatgc cccgcgcgga acggacgcgg tagccgacgg caataatcat gggcagggaa
     3721 taatgggcga ttttgcggtt taccttgcgc ccgttttcat tttgaactgt caactggaag
     3781 ttgacagttg ccttctcttc caattcttgc tctgcaagaa tggttttaat gtgtttgctg
     3841 atattttgtt tggtggtttg gtacagttct gccatgtccg cctgcgtcag ccaaagctgt
     3901 ccgccaaatt cctgcaaggc aaattgggca gtgccgtcgt tggcggtgta taggatgatg
     3961 ctcatgcttc aatctctgca atcacttcgt caatttcacg ccgcagccgt tcgattttgg
     4021 cgacggtttc gccgatttcg gcgttgagct gtttgatgtc gataatttcg cgtgtgtctt
     4081 cggcttcgac atagctgctg acggcgaggt tgtagccgtt gtctttgacg gtttgctggg
     4141 cagcgttttg ggcgatatgc ggcacatcgg ctttatcggc gaagagtttg acgatttcag
     4201 caatgtgttc ttcgattaag acgttgttgt tggtttcttt tttaaagaag ccgcttgcgt
     4261 cgatgaattg gatgtcggta ttgtctttgt gtttggacaa aaccaggata ttgacggcga
     4321 tgccggtgcc gtaaaagaga ttgggcgcaa gggcaatcac ggtttccacg tagttgccct
     4381 ccaccagata ttggcggatt ttctgttctg cgccgccgcg atagaaaatg ccggggaatg
     4441 agacgatggc ggcgcggcct ctgccggaaa ggtagttcag tgcgtgcagg atgaaggcaa
     4501 aatcggcttt ggatttcggg gcaagtacgc ctgcgggggc aaagcggtcg tcgttgatta
     4561 aggtggggtc gtcgctgcct atccagttga tggaataagg cggattggaa acgatggcat
     4621 caaagggttt gctgtctttg agctttgggt tggtcagtgt gtcgcccaat tcgatgtgga
     4681 attggttgta attgacgttg tgcaggaaca tgttcatgcg ggcgaggttg taggtggtgt
     4741 ggttgatttc ctgcccgaag aagccttctt cgatgatgtg ctcgtcaaac tgttttttcg
     4801 cctgcaagag cagactgccc gagccgcaag ctgggtcgta gattttgttg actttctcct
     4861 gtccgtgcac cgccagccgc gcaatcagct tggatacgct ttgcggggtg aaaaattcgc
     4921 cgccggattt gcctgcgttg gcagcgtagt tggaaatcag gtattcgtag gcatcgccga
     4981 aaaggtcgat gtggtggttt tcaaaattgc cgaaatcgag ttccgccacg cctttgagga
     5041 cggcggcaag gcgtttgttc ttgtcggcaa cagtgctgcc gagccggctg ctggtggtgt
     5101 cgaagtcgtc aaacaggcct ttgatgtcct gttcggacgg atagccggag gcggagcttt
     5161 caatcgcggt aaaaatttct ttcagcttgg tgttgagctc ttcgttttga tgggcttcgg
     5221 cggcaatatt gcaaaaaagc tggccggggt agatgaaata gcctttaact ttgacggcat
     5281 cgtctttgat ttcgggcgtg atgatgctgt ccggcatagc ggcgtaatca atactgctgt
     5341 cgcctgcctg catatagtcg gtgaagtttt cgctgataaa gcggtagaaa agtgtgccga
     5401 gaacgtattg tttaaagtcc cagccatcca ccgcgccgcg tacttcgtcg gcaattttcc
     5461 aaatttggcg gtgcagttgg gcgcgttgtt gcatttcggt catcatcgaa atccatataa
     5521 aagttaaaca aatcaaaatc gcctgatatt ttcagacgat ttttttacgg gcattcaaat
     5581 taagccaaat tttccagcat ccatttgacg tagcggtcga cgcctgattt gacatcgaaa
     5641 aattcttcct tatatccggc ttcgcgcaat ttggtgatgt cggcttgggt gaagctttgg
     5701 tatttgcctt tgagcgcgtc ggggaaggga atgtagcgga taagttcttc ttctaccaac
     5761 tctttcaagc tcatttcagg tttgccttcg gcggcgcggc atgcgttgac ggtggcggcg
     5821 gcgagttcgt tgaactgttg gctgcggccg gtaccgaggt tgtagatgcc ggaaagttcg
     5881 ggatggtcga agaagtagag gttgactttg gcaacgtctt cgacgctgac gaagtcgcgg
     5941 gtttgttcgc cgttgccgta gccgtcgtta ctgccgaaca ggttgacgta accgtgttcg
     6001 cggtattggt ggaagtggtg gaaggcgacg gatgccatgc ggcctttgtg ttgttcgtgt
     6061 tgtccgtaaa cattgaagta gcggaagccg acgacttggg cggtgagacc ttctttcatg
     6121 cggcgacgca atacttggtc gaacaggaat ttggagtagc cgtacacgtt gagcggtttt
     6181 tcgagttcgc gctcttcgcg gaagatttct cctttgccgt aaaccgccgc actggaggca
     6241 taaaggaagg ggatgcgttc gtcctgacac cagtccagca aatccagcgt gtactggtag
     6301 ttgttgtcca tcatatacaa accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg
     6361 aaaacggctt cgatgttttg ataaggtaaa atgtgttccc tcacttggcg gatgaattcg
     6421 tgtttgtcga gataatgggc gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg
     6481 ctcaaattat cgacggcaac aatgtcagta ataccgcgtt gattaagtgc tttgacgatg
     6541 ttgctgccga taaagccggc cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt
     6601 tttgagtaaa attaataaac ttagttacaa gaaatcatcg atattgtcct gaaacacaaa
     6661 tttgcgatga tattccaaat atttttctct tccttcggga tatattctca tcttaatttt
     6721 ttgcatttgg attgatatta agtttttccc atgtgtaacg gcttagtcga gtaccgcaaa
     6781 tcaaacgacc atcatccaag aaagtaatat aaccttgatc gaacaaccaa tcataagtcg
     6841 gcgttaaagc taacccattt aaataatcta atgcctcttt ctcatttttc tcagtaatac
     6901 aaaccatata aggtttaata tgactagccc ttaacaaaat ctcatctgta attaatgtaa
     6961 atggacattg cgacatatga tttataacat ccttgcgata tttctgctgc ccttttctac
     7021 ttttctgttt aatgatttct tgctctacga attgattaaa ttctgaagcc ggtatttcta
     7081 atttttctct tgataagagt tgcgggtgca cgatagagcg aaattgataa tccaaaaata
     7141 ttcggaaata aaataatggt tctgaatctt ctatatcttt gacaggcaat agtttaagaa
     7201 ttgataaata actaattttg ggcaatacaa tcttacgcca aattgaccat atatcatcct
     7261 ccgaacggat ataaccccta cggtaacctt tagaattttc caaatggtct gatacatcat
     7321 ggattgaaaa gaaaatattt tctgtaggca gattattaat acttaaaacg taatcatcat
     7381 aaaaccttga aatatcatga tgatatttct gttcttgttt gtgatattca acttttgcat
     7441 caaccaaata acgctgtaaa tttttcttat aaaaaaaaca atttttctga atgaaaggat
     7501 attctaccct ccttccattt tttgtctcag taatccccca attttcatac tcaaaaaatg
     7561 tacttagttt tcgaccacta tcatcaatat aactacctac atatttgcgt gcttccccat
     7621 taccaccaaa aacagataac ttgttattcc tgtgaataaa actatcttcc gcacgtaaat
     7681 tttgaatact atcgacaata aaatactcta tgttttcaat agtaattctt tgaaaatccg
     7741 gcattttact ctccataaag cgtaatatcc atttgtttta atatagcgat taatacatca
     7801 acaacaatac tgtttcccgc ttggcgatac atttgagtat cactaactaa aattttaaaa
     7861 tcatctctaa aacccattag ccgcaaacat tccctaggtg ttaatttacg aatacgtcca
     7921 cgattatgag taacataatt atcaaccccg gcacgatgca ttttatgcat agtttgcaat
     7981 aatgggcgag ctacaggcaa atcagtttcc gtactggttt taaaattttt tgttcctcct
     8041 gccaaaacat aatttttgat tttttcagaa agataatatt tttcctcaac gttatttaca
     8101 tcaaaaataa agtcatcaaa ttcagattcg cgggctgcct gaaaaataaa atcaccatgc
     8161 caattaaatt gttgatttgc tttttggcat aaggcaattt ctccattaat ctgtgtataa
     8221 cgtttttgtc tatttttaga actggtaaca aatttcgcac ccttttcacg taaaaaatat
     8281 ttgctatcag tatagtcctc caaaaaatct tgcatagtat gttctaattc aatcttttca
     8341 ggaaactgaa aaccatttat aggaggggta tgaaagccaa caacaaaaat acgctcacga
     8401 tgttgaggaa tcccataatc cttactattc attatttgga aatataagtc ataaccaagt
     8461 gaataaaaaa cactttttac aactttccaa gtttttccat tatcatgatt aagcaagccc
     8521 tttacatttt cataaataaa tatctttggt tggacttcat ccacaacgcg agcaaattca
     8581 tagaataatg ttcctcgtgt atcttctaat cctgcacgtt tgccaaccat ggaaaatgct
     8641 tggcaaggac tgcctccaac taaaatatca acttgatttc taaactttct cgcatcaaat
     8701 tgagtaatgt cgttatgcca aaaatcttca tttaatttat agtttccaag ataacttttt
     8761 ttaacgtatg gatcaatatc tcctgaaaaa acaatggtat ggtttaaatt taatcggtgg
     8821 aatgcctgtt caaccgcacc aatcccgctg aatacggttg ctaatctaat atgtgaatca
     8881 ggtttaagaa aagttttaga tttccaacct tgttgactgg gaaagagcaa agttttttgt
     8941 aatcgagtat cgtgtgtctg tgccattgtc gaaatagtca tacttatatc gttctgttta
     9001 tcttatcaat atgaaaacta cattgttgat tgccctgaca atgccttgat caattcggca
     9061 aacgagcaaa ccgccgtacc gagtttcgcc acgacaaccc cggccgcagt attggcaagg
     9121 tacatggctt cgggcatggt gcagcctgcc gccaaaccca agcccattcc ggcaatgacg
     9181 gtgtcgcccg caccggatac gtcgtaaact tcttgggcgc gggtgggctg gtaaatcggt
     9241 tcgccttcgc tgaacaaggt catgccttct tcgcttcggg tcagtaaaac ggcggtcagg
     9301 tcgaggtggc ggcgcaggtt ttgcgctttt tcggtcagct cgctttcgtt tttccaactg
     9361 ccgaccactt ctttcaattc ggcgcggtta ggcgtaatca gagttgcacc gacatatttt
     9421 tcgtaatcgt cgcctttggg gtcgattaag acggttttgc cggcgtgttt cgcccaatcg
     9481 atcatatcgg agatatgcga caggccgcct ttgccgtagt ctgaaaaaat gattgcgtcg
     9541 tattcgggca agatttcgcg gtatttctgc ttgatttgtt ccaacacttc gcagttggga
     9601 tgttcttcaa aatcaagacg gataagctgc tggttgcggg cgacgacgcg cagtttgacg
     9661 gtggtggcga tttgtttgtc gcgcatcaga taggaggcga cgccgtcctg caccatcagc
     9721 gcatcgagcg cgtcggcggc ttcgtcgttg ccggttacgg acaacagccc tgccctgccg
     9781 cccaacgaag cgatgttgcg cgcgacattt gccgctccgc ccgcgcgttg gtcgattcgt
     9841 ccgattttcg ccaccggcac gggggcttcg ggcgaaatac gggacacatc gccgaaccaa
     9901 tagcggtcga gcatcacgtc gccgacaacc aggactttgg cttgcgcgaa acgggatttg
     9961 agggtttctt gttggaactt ggcggacata ttgcctccgt tgtatttttt cagacggcct
    10021 cagcctgtaa ggccgtctga aaatcagttt gcttcaaggt ttgccacgcg gttcacttcg
    10081 cgcaatacgg ctaccggatc ggcagcttgg gttacaggac gacccattac caaataagtc
    10141 gaaccggcag ccaaggcttc ggccggtgtc atgatgcggc gctggtcatc attattgccg
    10201 gcaacgtcca agcggatgcc gggcgtgacc aagacaaaat cctgtcccaa ttcgcggcgc
    10261 agcggcgcgg cttcttgggc ggaacagacc acgccgtcca agcccgaact ttgcgccagt
    10321 tttgccaagc ggatgacttg ttcttcaggg gcggtgttca aaccgatttc cgccaaatca
    10381 ctttgttcca tgctggtcaa cacggttacg ccgattaaga gcggcttcgt gccgtatccg
    10441 gcaacggctt ctgctgcggc ttccatcata cggcggccgc ccgatgcgtg catatcgacc
    10501 atccaaacgc ccatatcggc agcgactttg caggcttgcg cgacggtgtg gggaatatcg
    10561 tggtatttca aatcgaggaa aagtttgaaa ccttgattga ttaagctttc tgccaaattg
    10621 cgtcccgtcg cggtaaacag ctctttgccg attttgattt gacacaatgt cgggtcaagg
    10681 ttgcggacga atccgagcgt gtctttttcg ttggaaaaat caagggcgac gataacgggg
    10741 gtgcgttgtt gcggagtttg gaagtcggag attaaaggat tcatggtgtt ttcgtttcgg
    10801 gttttcagac gacctttatc gtctttttcg cagtatagtg gattaacttt aaaccggtac
    10861 ggcgttgtct cgccttagtt caaagagaac gcttctctaa ggtgctgaag cacaaagtga
    10921 atcggttccg tactatctgt actgcctgcg gcttcgtcgc cttgtcctga tttttgttaa
    10981 tccactataa caacgtaaat tcaaaccgta ggtcgggcat tgatgtccga cctacgcgga
    11041 ctcaagcctc cgacaaactg tttcggataa atttgaaccg gtacatcagc cgtcattccc
    11101 gcgcaggcgg gaatccattg ataaaactca gttcatcaga tttaaacagc gattgcccaa
    11161 attcagaaat ggattcccgc ctgcgcggga atgacgccaa atgattattt ttcggttgca
    11221 acacgctgtt tatcaaaaac aggtcgtctg aaaacctgat tttctgtttt cagagtgcga
    11281 agcctgcccg tgggtacgac cttttcgatt tatttgccca atgcgcccaa tacttcggct
    11341 ttcacggctt ctactgcttg ggtgccgtca actttgatgt atttaggcgc gtgttcgcct
    11401 tccagtttgc tgtaaaaatc gaccaaaact tcggtttgct cgtggtaaac ggcaaggcgt
    11461 tttttcacgg tttcttcttt gtcgtcgtcg cgctgaatca aatcttcgcc ggttacgtcg
    11521 tctttgcctt caactttggg cgggttgtag gtaacgtggt aagtacggcc ggaagccaaa
    11581 tgcacgcggc ggccgctcat gcggtcgaca atcacgctgt caggcacatc gatttcaacg
    11641 actgcatcca aatccacgcc tgcttcaacc atcgcttcgg cttgtgccaa tgtgcgcggg
    11701 aaaccgtcaa acaagaaacc gtttttgcag tcgtcttgcg cgatgcgttc tttgaccatg
    11761 ccgataatga tgtcgtcgcg caccaagccg ccttcgtcaa tgattttttt cgcttccaaa
    11821 cccaagggcg tgcctgcctt aatcgcggca cggagcatgt cgccggtaga gatttgcgga
    11881 atgccgaacg ctgcggtgat gaattgcgcc tgagtgcctt tgcccgcgcc cggcgcgcct
    11941 aaaagtaatg ctttcatgaa gtgatccttt gaaatgtgtt tattggaatc aaacagatgg
    12001 tttacgattc tacacggctt ggcacaatag ttcaaaaacc aactgcacaa actgccattt
    12061 gcaagcaaat gttttgcata cctttgccga gtctgatatt cacaagccgg caactttttg
    12121 ggacaatccc ccaaaatttc aaaacatcac accctaccaa aaaggaacat ccgtgaaacg
    12181 aatttttctg tttttggcta ccaatatcgc tgttttggtc gtaatcaaca ttgttttggc
    12241 ggttttgggc atcaacagcc ggggcggcac gggcagcctg ttggcgtatt ccgccgtcgt
    12301 cggcttcact ggttcgatta tttcgctgct gatgtccaaa tttatcgcca aacaatcggt
    12361 cggcgcggaa gttatcgaca cgccgcgcac cgaagaagaa gcctggcttt tgaacactgt
    12421 cgaagcccaa gcgcggcaat ggaacctgaa aacgcccgaa gtcgccatct accactcccc
    12481 cgaacccaat gcctttgcca cgggcgcatc gagaaacagc tccctgatcg ccgtcagcac
    12541 cggtttgctc gaccatatga cgcgtgacga agtggaagcc gtattggcgc acgaaatggc
    12601 acacgtcggc aacggcgata tggttacgct gacgctgatt caaggcgtgg tcaatacctt
    12661 tgtcgtgttc ctgtcgcgca ttattgccaa cctgattgcc cgaaacaacg acggcagcca
    12721 gtcccaggga acttatttcc tggtcagcat ggtattccaa atcctgttcg gcttccttgc
    12781 cagcttaatt gtcatgtggt tcagccgaca acgcgaatac cgcgccgatg cgggcgcggc
    12841 aaaactggtc ggcgcgccga aaatgatttc cgccctgcaa aggctcaaag gcaacccggt
    12901 cgatttgccc gaagaaatga acgcaatggg catcgccgga gatacgcgcg actccctgct
    12961 cagcacccac ccttcgctgg acaaccgtat cgcccgcctc aaatcgcttt aaaccgattt
    13021 gaaacggcaa aaaccgtacg cgcaagcagt acggtttttt gtatatgctg tctgaaaaca
    13081 tcaggtttcc ataccgacac caaccgtatc catttccgca ccggatacgc cgcattgata
    13141 taccctgata aaccgtatcc ggacagaaaa tatagtggat taacaaaaac cagtacggcg
    13201 ttgcctcgcc ttagctcaaa gaaaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
    13261 gttccgtact atttatactg tctgcggctt cgtcgccttg tcctgatttt tgttaatccc
    13321 ctatatcaga taaaacagcg gtaaaattgc cgtctgaaac caactctgcg ccatacggga
    13381 cacgctctgc acgtctattt gcctccgata tatcctccct actgtaggaa cgtctgccga
    13441 tttgcacgaa atcggcacct ctagcaccct tcaggatggc ggacgttatc cgttcttggg
    13501 attctgcaag acggtcgacg gcattataca gacgctcggt ctgataccct tccgaagcaa
    13561 cctgcttcag gtattccaaa taaccgtaac ccaacgcccc tccgacaagc agggtcgtca
    13621 acgctaccag cgttgccgaa cggattttag ggggcatatc ttgaagcata tctctaccga
    13681 accgaaccaa agtctcttta aagtcggact ctacacgcgt agaaccctcg gaaatcttga
    13741 agttcaactc atactgcttc cgtttgtccg ctgtcaaccg cttgaggttg tcatccccgt
    13801 attggacggt tgacacaacc ctgtacagac tgtcctgtat ttcgaccagc gtctgtgcaa
    13861 agttggcggt cataactgga ttcccaaccc ttgccgccga ttctgatact caaaacggta
    13921 tccaggcgtt ccagcagggc ttcgggagac agcagtgcgt attcttcaat attttccata
    13981 ggttgattat agcagaacct tatacctgtt acagacaagg ttgtcgggat ttgaacgtcc
    14041 gtcgagcttg gttcgtatag agccgtaggg ggtgtttagg gctttggcgg cagcttgtac
    14101 gctggggaac gtgagtgttt cgcctgtggc ggtgtcgagg atggagacag gtttttggtt
    14161 ttggggaggc gtattttcct tcattttaag tttcccgcac tccggacacc catactttga
    14221 ctttatagct gtgtcgtaat acaaaagttg gacaaccccg tgcgtaggac atctaacact
    14281 ggaaggttta taaatgccac tgaactccaa caattctaaa tgtggaaatt tctcttgaaa
    14341 gcgtgatttg gcggtatcga aagtcattcg cttacccata atttgtctcc ataggttatg
    14401 tgcaaatgga ttacacaacc aaaatataga aattttcaaa ttttttgaga cctacctgaa
    14461 atcctgcgca agcacaatgt aaagaaaaat ggtatacgtc tttactaaat ctttacataa
    14521 attaactcta accgggattt gaacgtccgt cgagcttggt tcgtaggatt tagggtgttg
    14581 tgaaccttac ctatcgtacc caagcacttt gtacctgttt gaaataaggt tgtcgggcga
    14641 cgttcgaccc ttgagcctgt gattgatttg ttggaaatgt acacctaaag cagcacctgc
    14701 ggcagtaacg cttgggaacg tgagtgtttc gttggtcgtt gtgtcgagga tgaggagggg
    14761 cttccctaca tttggaggtg tttgggtttt tgacatctta gcacactcag gacacccgta
    14821 ctttgacttt ataagggcat taaatgtcga ataacgaacg ttcccgtgca taggacaatt
    14881 aacggttact gggtaattca caccgtcaaa gccggttaga gagatattag ggaatatatc
    14941 agtaagttca gaagctgctt ggttgaaaga tttggccatt tgagactcca aaattgattg
    15001 tcaaatggat ttaatagcaa atttataaaa atgtcaaatt tttcgagaag cacctgatag
    15061 tggcgagggg tatacactgt taaaaagtgt atacgtcatt tagtgcagat gtgaacctta
    15121 tacctccctg caagtaaagt ctctgtgtga acccgacctt ttatacggtc agtgatagct
    15181 gctgggttta tacccaatga ccttgcagca gatgctcttg aggggaatgt aagtgtttcg
    15241 ccagttacag tatcctccaa tactgtattt ttagataatt tatctattgt atcttctgga
    15301 attttgtggg aattgacacc ataagatgca cattttggac acccactttt agagtccaac
    15361 atacttttga atgtagagca agtaaccctc ccgtgtatag gacagacgat taccgtcggg
    15421 taacgaacgc cattgaactc cacaagattc aaatgtggaa atttagaggt tagcttagaa
    15481 actgcttgag taaatgtaag tgccatagtg gttgtgaact tactttacca acctataacg
    15541 atacctaatc aatcggtcaa acggagtgcg tccgcttaaa cgtacagcta aagtgctgtt
    15601 ggagcaaccg ataaatttac tggcagccag tgtacttgca aactcgtaga tttcccctgt
    15661 ctctgtgtcc tccaacctaa tggcaacggc gtttggagag ggcttttcgg gatttcgagt
    15721 acattcagga catcctgttg aggatttcat ggcagcttgt agattcccgt aaacaaccga
    15781 cccatgcaaa gggcattgga ttgttaccgg ttcgtagttt ttagtgaact tcaaaaccgt
    15841 cttatcagga aatttactgg aaaaccttaa ctgaacatct tgtagcgaga gtgattttgg
    15901 cattatagac ctttctaaaa tgaaagcatt atacaaccaa aatatagaaa ttttcaaatt
    15961 ttctctgaac gcactggtat ccgatggagc taaatgtaaa gaaaagggta tatgtcctta
    16021 ctaaatattt acacatttta actgtatagt gtttgacaaa aaaacaggca taggcgcata
    16081 atgcacccgt agttagatag atagtaagga gataaccaat gacttagcac ctgcccccct
    16141 ctctctcgcg caccccgccc ctcgcttgcg gctaatgggc tttgcgcgac atatacactt
    16201 tcatctcctg catggcttaa ccgcctatgc aatagtacac acatcaacaa atcaggctgg
    16261 cacgtcttgg cattggctga cgtaccattg tagcccgaaa tgttgatacc ctactattta
    16321 tgaccaacgg cggattaggg cttcagacgg caattttact gcaaaacctt atgcgaacgg
    16381 atgatgcagc acaatcgttt cttctcggtc ggggccggtg gagacgatgg cgaccggcgc
    16441 gccgcagact tcttcaatcc gtttcaaata tgctttggcg ttttcgggca atgcgccgta
    16501 gtccttcacg ccgaaagtgg attcgcgcca gccgggcatg gtttcgtaaa tcggcttgca
    16561 ggtttccacc gcatcggaac cgcaaggcag gatgtcggtt ttgccgccgt cgggcaattc
    16621 atagccgacg cagatattga tggtttcaac gccgtccatt acatcgagtt tagtaataca
    16681 cataccggaa atgccgttga tttggatgga gcgtttcagg gcggcggcat caaaccagcc
    16741 gcagcggcgt gcgcgtccgg ttaccgaacc gaattcgtgt ccgcgttctg ccaaacctac
    16801 gcctacttcg tcgaacaatt cggtcgggaa cgggcccgaa ccgacgcgcg tggtataggc
    16861 tttgacgatg cccaaaacat aatccagcat ttgaggacct acgcccgcgc ctgccgaagc
    16921 tgcgcccgcc agacagttgg acgaggtaac gaagggataa gtgccgtagt cgatgtccaa
    16981 caacgcacct tgcgcgcctt caaacagcag tttttcgccg tttttgtttt tctcgttcaa
    17041 cacgcgggac acgtcggtaa tcatcggcgc aatgcgcggc gcgacttttt cgataaccgc
    17101 catcacgtct tccgctttaa ccggctcggc attgtgcaga tgttgcagtt ggacattgta
    17161 ataggcaagg acggcatcca gtttttcacg cagtttttca ggatgcagca aatcggcggc
    17221 gcgaatggcg cggcgtgcca ctttgtcttc gtaggcaggg ccgatgccgc ggccggtcgt
    17281 gccgattttg cctttgccgc gcgatgcttc gcgggcttgg tcgagcgcga tgtggtaagg
    17341 caggatcagc gggcaggtcg gcgcgatttt cagacggcct tcgacgtttt tcacgcctgc
    17401 cgcgttcaac tcgtcgattt cgcccaacag ggcttcgggg gagacgacaa cgcccgaacc
    17461 gatgaagcag tccaaacttt catgcaggat gccgctcgga atcaggcgca aaatggtttt
    17521 tttgccgccg acaaccaagg tatggcccgc attgtggccg ccttggaagc gcaccacgcc
    17581 gccggcttct tccgccagcc agtcaacgat tttaccttta ccctcgtcgc cccactgtgc
    17641 gccgattact acaacatttt tagccatagc catataacct atcgatatta aaaattattg
    17701 cggaaatccc caaaccggtg cggctttgcg ccccgccacg atttgccttt catccgccat
    17761 gaacgggtat ttagcgtttc acgacctgcc aaacgccgtc cgtctttttc agacggcctg
    17821 caagctcttc cgaaacattg tgtccgatac cgtaatcgat tacgacacac tgcccttgtt
    17881 cacgcaaggc ttcgaccgct tcgtgcgccg cttcggcatc ttccgcatcg accaacacgg
    17941 cgggctgccg ttcgatggcg ggcaaacgcc cgataaagct gcgcaagtcg aaactgaatc
    18001 ccgttgccgg gcgcgcccta ccgaaatatc cgcccaatcc gtcataacgc ccgccgcgcg
    18061 cgaccgcgtc gtggaaatcg gcggcatagg cggcatacag caagcccgtg tggtaattgt
    18121 cgacacgcag ctcggacaag tcgatatgga tttcacaatc ggggaatgcg tcgcacaccg
    18181 cctgcaattc gcccaacgcg ccgccgaccg ccgacaaatc cggcaaccgt ccgcgcgcgt
    18241 cggacaacac ttcacgcccg ccgtacaggc gcggcaacag cgagaatgct tttgcccaca
    18301 tgccgtccag cttccaagcc ttgacctgcg cttcgaccgc cccggtatct ttatcctgca
    18361 tcaaggcaag cagcgttgcg gactgccccg catccaaatg tgccgcatcg gacaaggcgc
    18421 gaaatatgcc gatatgcccc agcgaaagca gcactttgcc catatcggca attttcatgc
    18481 ttttcagcat caggtctatc agctcgatgt cgccacggat gtcagcaaaa ccgtacattt
    18541 ctgcccctgc ctgcaagggt tcgcgcatat tcagcagacc gtcgggctgc gcgtgcaaca
    18601 ccggaccggc ataacacaac cggttaatcc cttggttggc ggataaaaga tgggcatcga
    18661 tacgcgccac ctgcggcgtg atgtcggcgc gtatgcccaa ctgcctgccg ctgagcctgt
    18721 ccgttaccaa aatggttttc agggaaagcc ccgcatcgat atgcgtcagc agggaatgtg
    18781 cgtactccat cagcggaggc tgtaccagtt cataaccgtg tacgcggaac agtgccaaca
    18841 actgctccct cgcgctttca agctgccgcg cgttcgtggg cagtacgtcg gcgatatgtt
    18901 ccggaagctg ccatgtctgc atgagaatct gccttctatt ttatcctgta aacacaaaag
    18961 actgcccgat ccgcaatcag acggcatctt tgccgcaacg catcacgccg ctgtcaaaac
    19021 ggcggtgctt ctttccgatt cagaatccga acagcccctg tccgacggct caatgccgtc
    19081 aagtcgttaa aaccaaactt taccataaaa tacacacaat ctgaatatac ttttataccc
    19141 tgtatccgcc gtctgacggc acggcatttt tttacgatat aatgtgcacc attatttctc
    19201 atcacttcct gctgccgttt tctgcttcag acggcatttt tgttgattga acacttatgc
    19261 tgctcgacct caaccgcttt tcctttcccg tcttcctgaa agaagtccgc ctgctgacca
    19321 ctcttgccct gcccatgctg ttggcgcagg tcgcgcaggt gggcatcggt tttgtcgata
    19381 ctgtgatggc gggcggtgcg ggcaaggaag acttggcggc ggtggctttg ggcagcagcg
    19441 cgtttgccac ggtttatatt acctttatgg gcattatggc ggcgctgaac ccgatgattg
    19501 cccagcttta cggcgcgggt aaaaccgacg aagtgggcga aacggggcgg caggggattt
    19561 ggttcgggct gtttttgggc gtgttcggca tggtcttgat gtgggcggcg attacgccgt
    19621 tccgcaactg gctgaccttg agcgattatg tggaaggcac gatggcgcag tatatgttgt
    19681 tcaccagctt ggcgatgccg gcggcaatgg tacaccgcgc gctgcacgcc tacacttcca
    19741 gcctgaaccg cccgcgcctg attatgttgg tcagctttgc ggcgtttgtg ttgaacgtgc
    19801 cgctgaacta tattttcgtt tacggcaaat tcggtatgcc cgctttgggc ggcgcaggct
    19861 gcggactggc gacgatggcg gtgttttggt tcagcgcgct ggcattgtgg atttatatcg
    19921 ccaaggaaaa tttcttccgc ccattcggac tgacggcgaa attcggcaaa ccggattggg
    19981 cggtgttcaa acagatttgg aaaatcggcg cacccatcgg gctgtcttat tttttggaag
    20041 ccagcgcgtt ttcgtttatc gtgtttttga ttgcgccttt cggcgaggat tatgtggcgg
    20101 cgcagcaggt cggcatcagt ttgtcgggga ttctctatat gattccgcaa agcgtcggct
    20161 cggcggggac ggtgcgcatc ggcttttcgc ttgggcggcg cgaattttcg cgggcgcgtt
    20221 atatttcggg cgtgtcactg gtgttaggat ggatgctcgc cgtgattacc gtgctttcct
    20281 tggtattatt ccgttcgccg ctggtaagta tgtacaacaa tgatccggcg gttttaagca
    20341 tcgccgccac cgtcttactg ttcgccggct tgttccaacc ggcagacttc acccaatgta
    20401 tcgcctccta cgccttgcgc ggctacaaag ttacaaaggt gccgatgttc atccacgccg
    20461 ccgccttttg gggctgcggc ctgctgccgg gctatctgct cgcctaccgt ttcgatatgg
    20521 gcatttacgg cttctggacg gcattgattg cctcgctcac catcgccgcc atcgccttgg
    20581 tgtggtgctt ggaattgtgc agtagggaga tggtcagatc gcataaggcc gtctgaaaac
    20641 gcagtacact tcaattcaaa tacagttaaa gttcaaacca tgcaacccat ccgataccga
    20701 accgacctta ccccctacaa caccttcggt cttcgcgccc aagcccgggc ctttatcgcg
    20761 ctcgaacatg ccgacgagtt gcgcgacatc gtccgactgc tggagttcga ccgcgatact
    20821 gttttatggc tgggcggcgg cagcaacatc cttttgatgc aggattacgc cggactggtc
    20881 gtacacatgg aaaacaaagg catacgcgag attgcgcgtt cagacggcat ggttctgatt
    20941 gaagcgcagg cgggcgaaat ttggcacgat tttgtcctgc acaccgttgc gctgggtttg
    21001 agcggtttgg aaaacctgag cctgattccg ggtacggtcg gcgcatcgcc cgtgcagaac
    21061 atcggcgcat acggcgtgga ggcgaaagac gtgattcaca gcgtgcgctg ctttgatttg
    21121 gatacggaga cctttgtcga gcttgccaat gccgactgcc gcttcgccta ccgcgaaagc
    21181 ctgttcaagc aggagggtaa agggcgttat gtgattgttt cggtcgtatt tgcattaaaa
    21241 acgcattttg tgccgacttt gggttacggc gatttggcgg ccgccgttgc cgaactgagc
    21301 gcgggcaggg tcccgacggc gaaagatgtt tccgatgcag tgtgtgcaat ccgcaacagt
    21361 aaacttccta atcctaacgt gcttggcaat gtcggcagtt tctttaaaaa ccccgtcgtc
    21421 agcgcagaaa aagccgccac cttgttgcag cggcatcctg atatgccgcg ctatccgcag
    21481 cccgacggtt cggtcaaact cgccgccggc tggctgatcg accaatgccg tctgaaaggc
    21541 ttccaaatcg gcggcgcggc ggtacatgac aggcaggctt tggtcttagt gaacaaaaac
    21601 aacgcctcgg caaacgatgt ccggcagttg gcgcaacaca tcaaatttac agtatttgct
    21661 cggtttcagg tagaattgca cgccgaacct aattggctgc ctgcttcgtt cagcctgtaa
    21721 atccaaggag tatgcccgtg acccgccccg ccaaaatcaa tacttacaca cgcatcatcg
    21781 acgccagcct tgcgcttttc aacgaggaag gcgagcgcaa catcagcacc aaccatattg
    21841 ccgcccactt gggcatcagt ccgggcaacc tctattacca cttccgcaac aaagacgaaa
    21901 tcatcgtcca actgttcaaa cgttacagcg aagccctgct ggcatacctg aatgaagccg
    21961 tgttgccgtc tgatgtggaa gactccatca attatatggc cggtatttat gatgtgatgt
    22021 gggaataccg cttcctattc agcgacgtga acaccctgct tgcacgcagt gccgaattgt
    22081 tgggcgaaca caataccttc acccaagcca aagtctcccc gctcttggtc aacctgctca
    22141 cccaactcaa cggtctgaac atcatccaag ccgaccaaac cgccatgaac gatctcgccg
    22201 tcaatatgtg gatggtcacg aaatactggt tcgacttcga cagctccctg cgcggccgca
    22261 ccaagctgac cgaagactcc aaagcacgcg gcatccgccg caccttaagc ctccttcgtc
    22321 cttatctttt gcccgaacac cgcaagaaat acgaccggaa aatcggcaac ggcaacccgt
    22381 aaacccacag cgtccgaatc agccttcaga cggcacttac ccgacggtat cagcgaatgc
    22441 gcgcagtccc cccgccccat atctccatca caggcctagg ttacctcggc ctgccgctgg
    22501 cacaaaagtt ttaccaacac ggcagccgcg ttgccgccgt caaacgcagc ctgacttcgg
    22561 acgatatcaa tctgcccata cacctcgata ccatcgacct caatcaagac agcgcgtttc
    22621 aaagcgcgaa ccttgcccga gatacaagct tttggcggca ccatgccaac aaacccgttt
    22681 ggttctgcct cttgccgcca tcatcgctga cacattacgc cgataccgtc aaacaatggg
    22741 cagaacttgc ccgggcgtgc aacgtgcaac acctgatttt cacaagcagt accagcgttt
    22801 acggcgatac agcgcgcgaa tgcgacgaaa tcgccctacc cgatccgcaa accgagtccg
    22861 cccgccaaat cctcgccgcc gaacaacacc tgctcgacag cggcgttccg aacatcgaca
    22921 tcctgcggct gggcgggctt tattgcgccg aacgccatcc cgtcggccgc cttgttcaaa
    22981 agcaaaacat cccgggcggc aaccgcccca tcaacatcgt ccaccgtaat atcgccgtcg
    23041 aaagcctgtt tcagacggca tttaaccccg gcggcaggcg gctgaaaaac attatcgaac
    23101 cgcgccaccc gacacgacgc gaattctata cggaagaagc cgccaaactc ggcttgcccg
    23161 cgccggattt tgcacccgac gacagcgtgg gcaaaatcat ccgtaccgtt tgcgataacg
    23221 gcttaagcct gtaaaataag cggcaacagc aacaaacagg cattctccgc cccgatcaga
    23281 aaatatgtcc gccctcctcc ccatcatcaa ccgcctgatt ctgcaaagcc cggacagccg
    23341 ctcggaactt gccgcctttg caggcaaaac actgaccctg aacattgccg ggctgaaact
    23401 ggcgggacgc atcacggaag acggtttgct ctcggcggga aacggctttg cagacaccga
    23461 aattaccttc cgcaacagcg cggtacagaa aatcctccaa ggaggcgaac ccggggcggg
    23521 cgacatcggg ctcgaaggcg acctcatcct cggcatcgcg gtactgtccc tgctcggcag
    23581 cctgcgttcc cgcgcatcgg acgaattggc acggattttc ggcacgcagg cagacatcgg
    23641 cagccgtgcc gccgacatcg gacacggcat caaacaaatc ggcaggaaca tcgccgaaca
    23701 aatcggcgga ttttcccgcg aatccgagtc cgcaaacatc ggcaacgaag cccttgccga
    23761 ctgcctcgac gaaataagca gactgcgcga cggcgtggaa cgcctcaacg aacgcctcga
    23821 ccggctcgaa cgcgacattt ggatagacta accttcagac ggcatccgac atgaacagcc
    23881 cttttcacaa catcggcatc gtaacccgcc ccaacacgcc cgacatccaa gacaccgcac
    23941 acacgctgat tacctttttg aagcagcacg gctttaccgt ctatctcgac gaagtcggca
    24001 taaaggaagg ctgcatctat acccaagaca ccgtcggctg ccatatcgtc aacaagaccg
    24061 aactggggca atactgcgac ctggtcgccg ttttaggcgg agacggcacc tttctctccg
    24121 tcgcccgcga aatcgccctg cgcgccgttc cgattatcgg catcaaccaa gggcatttgg
    24181 gcttcctgac ccaaattccc cgcgaatata tgacggacaa gctattgccc gttttagaag
    24241 ggaaatacct tgccgaagag cgcatcctga ttgaggccgc actcatccgc gaaggcaaaa
    24301 ccgccgaacg cgccatcgcc ctcaacgatg ccgtcctctc ccgtggcggt gccggacaga
    24361 tgattgagtt tgaagtcttc gtcaatcggg aattcgtcta tacccagcgt tcggacgggc
    24421 tgattgtctc cacccccacc ggatcgaccg cctattcgct tgccgccggc ggccccatca
    24481 tgcaggcagg attacacgcc ttcacgctcg tccccatctg cccacaatcc atgaccaacc
    24541 gccccatcgc cattccagac acgtccgaaa tcgaaatcct cgttacccaa ggcggcgacg
    24601 cgcgcgtcca tttcgacggt caaacccata tcgacgtgca aaacctcgac cgcatcacca
    24661 tccgccgcta ccgaaatccc ttacgcattt tgcacccgac cgactaccaa tatttcaaaa
    24721 ccctgcgcca aaaactgcac tggggcgagc aattagtcta agccggcctt tacccgaaag
    24781 tatccatgaa tcccaaaatt tcctccgacc atacagaaga tgccgtgcgc ctctccaaac
    24841 gcatggcgca actggggctt tgttcacgcc gcgaagccga cggctatatc gaacagggtt
    24901 gggtaacggt caacggcaaa accgccgtac tcggtcagaa agtttcaccg gcagaccgta
    24961 tcgaactgaa caagaaagcc cacgaacagc aggcggcacg cattaccatc ctgttgaaca
    25021 aacccgtcgg ctatgtcagc gcacaagcgg aaaaaggcta taaatccgcc gccgaactga
    25081 ttacccctga aaatcactgg gaaggcgata ccggccgcat ccgtttcgat ccgaaacaca
    25141 aaatcggcct cgcccccgcc ggcaggctgg acatcgactc ggtcggattg ctggtattga
    25201 ctcaggacgg ccgtatcgcc aagcagctta tcggcgaaaa cagcggcagt gaaaaagaat
    25261 atttggtgcg cgtgcgcggc aaattggacg aaaaaggact tgccttactg aatcacggat
    25321 tgagtttgga cggcgagaaa ctgcgtcccg cccaagtaga atggcaaaac gaagaccaac
    25381 tgcgcttcgt gttgaaacag ggtaaaaagc ggcaaatccg ccgtatgtgc gaactggtcg
    25441 gactgcgcgt cgtcgggctg aaacgcatcc gcatgggcaa ggtcaaactc ggcaggctgc
    25501 cgcccggcaa atggcgttat ctcgctcccg gcgaatcgtt ttaaata
//
LOCUS       DAWISC010000009        24712 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWISC010000009 DAWISC010000000
VERSION     DAWISC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.009"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            26..1072
                     /locus_tag="WIW87_000271"
     CDS             26..1072
                     /locus_tag="WIW87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ5173490.1"
                     /translation="MLAILGALVFFINPGAIVLHRQIFLELMLPAAYGGFLTAALLDW
                     TGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCARLIWLDRNTD
                     NFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILLGAEALKECR
                     LKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAKLRELHHHEL
                     LRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGVMMVWLTAGL
                     WHSGFTKLDYPKLCRIAVPILFAAAVSRAFLMNVNPIFFITVPAILTAAVFVLYLFTF
                     IPIFRANAFTDDPE"
     gene            complement(1119..3308)
                     /locus_tag="WIW87_000272"
     CDS             complement(1119..3308)
                     /locus_tag="WIW87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ5173491.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDMARILSVQTAFVEEKPLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQQASDEIQTAFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWAQVLQQNRDGKIRWSVDVDPQEA"
     gene            3446..4228
                     /locus_tag="WIW87_000273"
     CDS             3446..4228
                     /locus_tag="WIW87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ5173492.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4641..6575)
                     /locus_tag="WIW87_000274"
     CDS             complement(4641..6575)
                     /locus_tag="WIW87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ5173493.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPDIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6641..7819)
                     /locus_tag="WIW87_000275"
     CDS             complement(6641..7819)
                     /locus_tag="WIW87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ5173494.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLSTGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8045..8434
                     /locus_tag="WIW87_000276"
     CDS             8045..8434
                     /locus_tag="WIW87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ5173495.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFDITLSEVSPDHFTLKATPNPTTND
                     GETCVVTLNDGGTIAASDTNQSCPGFD"
     gene            8717..9763
                     /gene="adhP"
                     /locus_tag="WIW87_000277"
     CDS             8717..9763
                     /gene="adhP"
                     /locus_tag="WIW87_000277"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ5173496.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(9928..13413)
                     /gene="smc"
                     /locus_tag="WIW87_000278"
     CDS             complement(9928..13413)
                     /gene="smc"
                     /locus_tag="WIW87_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ5173497.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSGHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGCLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCRLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13415..13666
                     /locus_tag="WIW87_000279"
     CDS             13415..13666
                     /locus_tag="WIW87_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173498.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            13663..14385
                     /gene="lpxH"
                     /locus_tag="WIW87_000280"
     CDS             13663..14385
                     /gene="lpxH"
                     /locus_tag="WIW87_000280"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ5173499.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14654..16240
                     /locus_tag="WIW87_000281"
     CDS             14654..16240
                     /locus_tag="WIW87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ5173500.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16409..16612)
                     /locus_tag="WIW87_000282"
     CDS             complement(16409..16612)
                     /locus_tag="WIW87_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ5173501.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPGNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            16728..17225
                     /locus_tag="WIW87_000283"
     CDS             16728..17225
                     /locus_tag="WIW87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ5173502.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIPSLRKQFPEFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17307..18500
                     /locus_tag="WIW87_000284"
     CDS             17307..18500
                     /locus_tag="WIW87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ5173503.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(18904..19839)
                     /gene="hemC"
                     /locus_tag="WIW87_000285"
     CDS             complement(18904..19839)
                     /gene="hemC"
                     /locus_tag="WIW87_000285"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ5173504.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            19958..20467
                     /gene="ybeY"
                     /locus_tag="WIW87_000286"
     CDS             19958..20467
                     /gene="ybeY"
                     /locus_tag="WIW87_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ5173505.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQED"
     gene            20475..21299
                     /locus_tag="WIW87_000287"
     CDS             20475..21299
                     /locus_tag="WIW87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ5173506.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHSKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(21428..22807)
                     /gene="nhaC"
                     /locus_tag="WIW87_000288"
     CDS             complement(21428..22807)
                     /gene="nhaC"
                     /locus_tag="WIW87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ5173507.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSSFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23098..24321)
                     /locus_tag="WIW87_000289"
     CDS             complement(23098..24321)
                     /locus_tag="WIW87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ5173508.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5659 a         7070 c         6350 g         5633 t
ORIGIN      
        1 gcggccgttt ttcgtcggcg cggcggtgct tgccatactc ggtgcgctgg tgtttttcat
       61 caaccccggt gccatcgtcc tgcaccgcca aattttcttg gaacttatgc tgccggcggc
      121 atacggcggt tttttgactg cggctttgtt ggactggacg ggtttttcgg gtaacctgaa
      181 acctgtcgcg actttgatgg cggcattatt gctcgccgca tccgctatac tgcccttttc
      241 gccgcaaact gcctcgtttt tcgtcgccgc ctattggctg gtgttgctgc tgttctgcgc
      301 ccggctgatt tggctagacc gaaacaccga caacttcgcc ctgctaatgt tacttgccgc
      361 gttcactgtt tttcagacgg catatgccgt cagcggcgat ttgaacctgt tgcgcgcgca
      421 agtgcatcta aatatggcgg cggtgatgtt cgtatccgtg cgcgtcagta ttcttttggg
      481 cgcggaagcc ctgaaagaat gccgtctgaa agaccctgtt tttattccaa atatcgttta
      541 taaaaacatc gccattactt tcctgctctt gcacgccgcc gccgaacttt ggctgcccgc
      601 gcaaaccgcc ggttttaccg cgctcgccgt cggcttcatc ctgctcgcca agctgcgtga
      661 gcttcaccat cacgaactct tacgtaaaca ctacgtccgc acttattacc tgctccaact
      721 ctttgccgcc gcaggctatt tgtggacagg cgcggcgaaa ttacaaaacc tgcccgcctc
      781 cgcgcccctg cacctgatta ccctcggcgg catgatgggc ggcgtgatga tggtgtggct
      841 gaccgccgga ctgtggcaca gcggctttac caaactcgac taccccaaac tctgccgcat
      901 tgccgtcccc atccttttcg ccgccgccgt ctcgcgcgct ttcttgatga acgtgaaccc
      961 gatatttttc attaccgttc ctgcgattct gaccgccgcc gtattcgtac tgtatctttt
     1021 cacgtttata ccgatatttc gggcgaatgc gtttacagac gatccggaat aaaaatgccg
     1081 tctgaaacgg ttttcagacg gcatcggatt gccaataatc aagcctcctg cggatcgaca
     1141 tccaccgacc atctgatttt gccgtcgcgg ttttgctgca acacctgcgc ccacaaactc
     1201 acggcacggt gcaaatcctg tcgggacgga gattcgagga agatttgcgc gcgttcgcgt
     1261 tcggcgaggc gcaccatcag catcggggcg gcgccgaact gtgaaacgct ttcgggcaaa
     1321 agcggggcga gggtttcttt ggcggcattg agaaactcca tcgcatcggc aacgcgcggc
     1381 gcgtcggcgc ggacggcggt ctggaaaccg aagggcggca tggcgaacat ttgccgctcg
     1441 ttcaattcgt tttcggcaaa cacggcgtag tcctgcgctt tgacggcggc gaagacggga
     1501 tgttcgggca gttgggtctg tatcaacacc ttgccgggtt tgtcggcgcg ccccgccctg
     1561 ccggacacct gcatcagctc ggcgaacagc ctttccgggg cgcgaaagtc cgcgctgtac
     1621 aggctgccgt cagcgttcaa cacgataacg aggttgagcc gcgcgaaatc atgccctttg
     1681 gcgagcatct gcgtgccgac caaaatgtcg attttgttgt cggcgatgcg gcggtacaaa
     1741 tccgcccagt cgtttttgtg cgcggtgctg tccctgtcaa cacggacgac ggctgccttg
     1801 ggcaggaagg tgcgcagggt ttcttcgacg cgctgcgtgc cgtggccgac ggcggtcagg
     1861 tcttggttgc cgcagtcggg gcatttgtac gggatgggtt cgcggtggtc gcagtggtgg
     1921 cagcgcagtt ggcgggcgcg ttggtgcagc accattttgg cggagcagtt cgggcagccg
     1981 aaggtataac cgcagtcgcc gcaaaacagc gcgggcgcga agccgcgacg gttgaggtac
     2041 accaacgaca tgccacctgc ttcaaagttc tgtttcaaaa gctgcaaggc ttgcggcgag
     2101 aagccgttgt caagtttcag acggcctacg ttgaggatgt ccacttgcgg cagttgcgcg
     2161 gcggtatggg cgcgttcggt cagttgcagc aggcggtacg cgccgctttg cgccttgtgc
     2221 cagctctcca agctgggggt ggcactgccc aatatgatcg ggcagccgcc ctgcttcgcc
     2281 cgccacaccg ccaaatcgcg ggcgtggtag cgcaattcgt tgtcctgttt gaacgagccg
     2341 tcgtgttcct catcgaccac aatcagcccg acatcatcca tcggcgtgaa caccgccagc
     2401 cgcgtgccga tgaccaattt cgcctgcccc aacatcgcgc gcaaataatc ctgcgtgcgc
     2461 ttgcctgccg ccatctgact gtgcaacacg gcggtcggca cgtcggcaaa acggttttcc
     2521 acccgcttca aaagctgcgg cgtgaggttg atttcgggca acagaaacaa cacctgccgc
     2581 ccctgcgcca acactttcgc catcgcatcg aaatacacct cggtcttgcc gctgccggtg
     2641 atgccgtaca gcaaaaacgg ctggaagctg ccgaaggccg tctgaatttc atcggaagcc
     2701 tgttgctggt cggcattcaa cacaaattca gagtgcgaag cctgcccgtg gtacgacctc
     2761 aatacaggtt tcgccgcttc cgttgtttca atccaaccct gctccgccca atcttcaatc
     2821 aatttcgccg cctgcgcgtt tacctgcttc aacgctgcca tcgtcattcc gcccgaaagc
     2881 agtgcgtccc acaaagccgc ttttttgttg aaccgagctg gtggcggcgt ttgcgccctg
     2941 cccgcttcgt tcaaagcata aaacaacggc ggctgcggca tttccaccgc gcgcgtttcc
     3001 ttcaaaccct gcggcagcgc ggcaaacacc gcctgcccag tcggatagtg gtaataacgc
     3061 gacgtaaatg ccaacaaatc acgccagctt tcaggcaacg gcttttcttc cacaaaggcc
     3121 gtctgaacac tcaaaatccg cgccatatcc atatcgggcg caatatccgt ttcccacacc
     3181 atcccgacca cggttttatt gcggaaaggc acaagcaccc gcgttcccgg aggaagcgga
     3241 tcggaatggg aataagtcaa aaggccgtct gaaagcggca cgtttacggc gatgcggtgg
     3301 taaatcatac aaacaagcaa atcatttttt gcgtatttta acagccggaa gctattttca
     3361 gacgataaat tgagcctagg cgcaatgcag gcgcaacaga gatttgatac aattccgctt
     3421 ttccaacacg cgcaatcatc tttatatgaa aaccaagtta atcaaaatct tgaccccctt
     3481 taccgtcctc ccgctgctgg cttgcgggca aacgcccgtt tccaatgcca acgccgaacc
     3541 cgccgtcaaa gccgagtccg ccggcaaatc cgttgccgcc tctttgaaag cgcgtttgga
     3601 aaaaacctat tccgcccaag atttgaaagt gttgagcgtc agcgaaacac cggtcaaagg
     3661 catttacgaa gtcgtcgtca gcggcaggca gattatctac accgatgccg aaggcggcta
     3721 tatgttcgtc ggcgaactca tcaacatcga cacgcgcaaa aacctgaccg aagaacgcgc
     3781 cgccgatttg aacaaaatcg acttcgcctc cctgcctttg gacaaagcca tcaaagaagt
     3841 gcgcggcaac ggcaagctga aagtcgccgt cttctccgac cccgattgtc cgttctgcaa
     3901 acgcttggaa cacgagtttg aaaaaatgac cgacgtgacg gtttacagct ttatgatgcc
     3961 cattgccggc ctgcaccccg atgccgcgcg caaggcgcaa atcttatggt gtcagcccga
     4021 ccgcgccaaa gcgtggacgg attggatgcg taaaggcaaa ttcccggtcg gcggcagcat
     4081 ctgcgacaat cccgtcgcgg aaaccacttc cttgggcgaa caattcggct tcaacggcac
     4141 gccgaccctc gtcttcccca acgggcgcag ccaaagcggc tacagcccga tgccccaact
     4201 ggaggaaatc atccgcaaaa atcaataatc catccgcaaa aaaggcttgg aaaattccaa
     4261 gccttttttg cgggaaatgc cgcctgaaaa ccaatccgcc ctgaaagtta gcaagccaac
     4321 aaacacatcc taacccattg aatgattgaa aataataaat tgaacaatac gaagccccgc
     4381 cgccgttccc acgcgggcgg gaatctggaa tattgagtta ccgtgattta tccgaaacaa
     4441 gcaaagccga acgaaccgaa ttcccgtctt tacgggaatg acgggactcg agcgttgtgt
     4501 gcaatcatcg aacccccgcc gccgtcattc ccacgcaagt gggaatctgg aacgtagaat
     4561 ctaagaaacc gtttttatcc aataagtttc tgctccaaaa tgaccggtcc tgcagcttca
     4621 gacggcatct ttgtccaacc tcaatcctgt gccaatgcgt ctatcggatt gagtttggct
     4681 gctttattgg caggcataaa gccgaacgcg atgccgattc cggtcgaaca ggcgaccgcg
     4741 ccgatgacgg acatggcgga aatgtccatc gggaagtcgg ttacaaaatg attgaacacg
     4801 aggctgacgg cggcggacaa acccacgccg accaaaccgc cgatgacgca gattaacacc
     4861 gcctcaatca aaaactgctg caaaatattg ccgcgccgcg cgccgattgc catccgtatg
     4921 ccgatttctt tggtgcgctc ggtaacggac accagcatga tgttcatcac gccgatgccg
     4981 ccgactacca atgaaatcag ggcgatggag gaaatcagca gcttcatcgt accggtggtg
     5041 ctttcgacta tctgcctgat gctgtcgctg ttgttcatga agaaatcttc cgtgccgtgc
     5101 cgcgctttga gcagatcggt cagccctttt tcggcaacct gggtattggc attgtctttg
     5161 attttgacgg tgatggagtt ggtgtggctc tcgcctgtga tttggtgcat caccgtcgta
     5221 tagggcgacc aaagcatcag cacgtcggaa ttgccgaaag cgttttcgtc ttttttcatc
     5281 acgccgatga cggtcaaggg gcgtttcctg aacaaaatgg ttttacccaa cggatccgag
     5341 tccgcaaaga gtttgtcttt gacattttgg tcgatgacga cgacctgcgc gtcttctttc
     5401 acatcgtttt cgtcaaacag ccgccccgtt tccagcttca gtccgcgcac gtcgaaatat
     5461 tgttcgccca cgccgtaaag cgaggcggtc aggtcggtgt tgcggtaagt cagcgtgccg
     5521 ccgctcgaag tcatgggcgt ggcggaagca acgtagcttt gtttggcgat gatttttgcg
     5581 tcgtctatgg tcagggtttt aatcctgccg ctgcgcctgt cgccgaagcc gcgccccggg
     5641 aagatgctga tggtgttcgt ccctatcgaa ctgatgtctt caaggatttt tttctgcgaa
     5701 ccattgccca atgcgacgac ggaaaccacc gacgcgatac cgatgatgat gccgagcatc
     5761 gtcagaagcg aacgcatttt gtgcgccaat actgcttgca ccgacattct gaaggcttcg
     5821 acaaactggt cgtaataaaa cgaccacgaa gctttttccc gaatcctccc gacattgctt
     5881 gcggggattt cgggattttt cgaggtgtcg gaaatgattt cgccgtcccg gatttcgatg
     5941 acgcggttgg cattggcggc gatgtcgggg tcgtgcgtga ccataatgac ggtatgcccg
     6001 gcttcgtgca gcctgcggat gatttccatc acgtttttgc cgctggcggt atcgagcgcg
     6061 ccggtcggct cgtcggcgaa gatgatttct ccgccgttca tcagggcgcg ggcgatggag
     6121 acgcgctgct gctgtccgcc cgagagttcg ccgggcttgt tgccctcttt gcttgccaaa
     6181 cccaaatcct gcaagagttt gtccgcccgc gcggaacgct ctttgccgcc cgcgcccata
     6241 tagacggctg gcagcgcgac gttgtccctt gcggtcagcg agcttaagag gttgtagcgt
     6301 tggaagatga aaccgaagcg ttcgcggcgc aatgccgcca gctcgtcagg ctgcattttg
     6361 gcagtttcga tgccgtcgat tcggtacgaa ccggaaccgg cggtatccaa acagccgagt
     6421 atgttcatca gcgtggactt gcccgaaccg gactgcccga tgatggcgac aaaatcgccc
     6481 ttctctatcg acaggctgat gtctttcaaa atatggacgc ggttctcgcc gctgccgaaa
     6541 tagcggttga tgtttttaca ttcgatcaag ctcataatat tccgtaaatc aataacaaat
     6601 gccgtctgaa accgtttccg tgttcagacg gcatattcgt ttatcggcgc ggcgggccgc
     6661 ctagggcgcg ttcgccgctt tcctgttgct cggcggcggt tatttcggag atgaccactt
     6721 tgtccccctc tttcaacccg ctttttactt cggtattcat actgtctctc ataccggtcc
     6781 ggatttcgcg ttccgccgcc ttgccgtccg cacccaacac gcgcacaaac gccttgccgc
     6841 cgcgattttt cacggtcagc gacggaataa tcagcacatt tttcacgccg tcgatttcaa
     6901 ccgtattctg cgtcgtcatc cccgtggaga gtttgccgtc cggattcggc acaaacgaac
     6961 gggcataata gtagaccgca ttggaagccg tatccgtact gctgttgtag ccgcccgacg
     7021 acatcgtggt cagcccgggg tcgacgctgt cgagcttcgc cttaatcggc gtatccggtt
     7081 cggacaaaat cgtaaacgaa atatcctgcc ccgccttcac cttggtaata tcgccctcgg
     7141 caatctgcat tttgttcaac atcatatcca gattcgccaa ttggacaatc gtcggcgtag
     7201 actgcgccgc gttcacagtc tgcccctctt ccacgagaat cgccaccacc gtgccgtcca
     7261 tcgttgcggt aatgcgcgtg tagcccaatt ccgactcggc ggtattgatg gaaattttgc
     7321 tctgtctgat taaagccttc agctcggcaa cattggcttt ggcggcggca aacgcatcct
     7381 gcgcgctttc caaatcctct ttggaagtcg cgttttcctt ccataacgcc gcctgacgct
     7441 tatatttctt ctccgcgctg cccaatgcaa tctgtgccga caccagcttc gcctgatacg
     7501 tttccaactt ggatttttcc gtattgagcg tattggtctg cgaggtcgaa ttgatttccg
     7561 caatcaaatc gcccttttta acctgttgcc cgagtttgac ataaagtttc ttaatctgcc
     7621 ccgatgcctg cgcgccgacc gataccaggt tggacggcga aatctcccct gttgcagaaa
     7681 ccgtccggct gatgtcgccg cgcctgaccg tttccgtaat ataagcagcc tgcggctcgg
     7741 gcttcagata agaccatccg ccccaaaccg ctgccgccgc gactgccgca acagccgccc
     7801 atttcatcat ttttgccatg ttttcaattc tctgtatgcg tcaatcccat aaatcggaag
     7861 cggattctac cgcaagtccc tgccccatcc aaggcggcaa actgccgaca ataaaaataa
     7921 ccatttgttt taaatggctt atacaatctg ctttcccgaa aatttcaatc cgtttacata
     7981 ttccgccatt tccgatacgg cgttataatg ccgtcgaccg acaaacaaat ggaaacacat
     8041 tgctatgaaa aacgttcaaa aaggctttac gctgctcgag ctgatgattg ccgtcgccat
     8101 cctcggcatc ctgacgctca tcacctaccc ttcctataaa acctacatcc ggcgcgtccg
     8161 cctgtcggaa gtcaggacga ccctgctgca caacgcgcag accatggaac gttactaccg
     8221 ccaaaaaggg acgtttaaaa cctacgacaa aaacaaactg aaacaaaacg aatatttcga
     8281 tatcacttta agcgaagtca gccccgacca ttttaccctt aaggcaacgc ccaatcccac
     8341 caccaacgac ggcgaaacct gcgtcgttac gctcaacgac ggcggtacca ttgccgcttc
     8401 cgatacaaac caatcctgcc ccggcttcga ctaatcctcc gccgaagccc gcaaacccgt
     8461 aaagatgccg tctggacaca gcttcagacg gtatctttac gggtttgccg tccggttttt
     8521 caaacttgaa acctgccgcc cctcatccgg caaccgcccc gatgccgcgc ctttccaagc
     8581 tgttataata taacatataa tctatatttt tcaacaacat accacttgac ctgtgagatg
     8641 cggaagcgta tgcttaccct tatatcggac aacattccgt ttacaggttt ttttgccgta
     8701 actaaggagc gcaatcatga aaatgcaggc agttgttgtg aataaaaatg tagcgggcga
     8761 tgtggaagta atcgaacgcg aggttcgccc gttggaatac ggcgaggcat tggtcgaagt
     8821 cgaatattgc ggcgtgtgcc acaccgacct gcacgttgcg gcaggcgact acggcgaaaa
     8881 accgggccgc gtgttgggac acgaaggcat cggtttggtt aaagaagttg ccgacggtgt
     8941 gaaaaatctg aaagtcggcg accgcgtcag catcgcttgg ctgttccaaa gctgcggctc
     9001 ttgcgaatac tgcaataccg gccgcgaaac cctgtgccgt tccgtattga acgcgggcta
     9061 caccgccgac ggcggtatgg cgacccactg tatcgtgagt gccgattacg cggtcaaagt
     9121 ccctgaaggt ttggatcctg cgcaagcttc cagcattact tgtgccggtg taaccactta
     9181 taaagccatt aaagtttccg gcgttcgtcc gggacagtgg attgccatct acggcgcggg
     9241 cggtttgggc aacttggggg tccaatacgc gaaaaaagta ttcggcgcgc acgttgtcgc
     9301 catcgacatc aacgacgaca aactggcgtt tgccaaagaa accggcgcgg atttggttgt
     9361 caacgccgcc aaagaagacg ctgccaaagt gattcaggaa aaaaccggcg gcgcacacgc
     9421 tgcggtcgta accgccgtat ctgctgccgc attcaactct gccgtgaatt gcgtccgcgc
     9481 gggcggacgt gtggttgcca tcgggctgcc gccggaatcg atggatttgt ccatcccgcg
     9541 tttggttttg gacggcatcg aagtggtcgg ctctttggtc ggcacgcgca aagatttgga
     9601 agaagccttc caattcggcg cggaaggttt ggttgtgccg aaagtccaac tgcgtgcttt
     9661 ggatgaagca cccgccattt tccaagaaat gcgcgaaggc aaaatcaccg gccgtatggt
     9721 gatcgatatg aaaaaagaat gcggctgcgg ccatcaccac tgatttgacg tggcagtaca
     9781 catcgaaatg ccgtctgaac gctgttcaga cggcattttt tatggatttg gatttgattt
     9841 taatctgttc tgtttgaaat accgtctgaa aacccatatc gcaacacttc attaaaaacg
     9901 gcaagattca gccgttctgc aacccgttca aacggcttcc gccatttcca acgcctgttt
     9961 gatgtccacg gcgacgacgc gcgagacacc tttttcctgc atggttacgc cgaccagctg
    10021 ctccgccatt tccatcgtca ggcggttgtg ggagatgtag aggaactggg tttgcgccga
    10081 catttctttg accagcctgc agaaacgcga ggtgttggcg tcgtccagcg gggcatcgac
    10141 ttcgtccaaa aggcagaacg gagcggggtt gaggctgaac agagcgaaca cgaggctcat
    10201 ggcggtgagg gctttttcgc cgccggagag gaggtggatg gtgctgtttt tcttgccggg
    10261 cggacgcgcc ataatggaca caccggcggt cagtaggtcg tcgcctatca ttttgagagt
    10321 ggcttcgccg ccgccgaaca gggtcgggaa gaaggtttgg actttgctgt tgacggcatc
    10381 gaaggtttct ttgaaacgcg ctttggtttt gtcgtcgatt tgggcgatgg cttcttccaa
    10441 aagggtgatg gctgcctgca cgtcttcgct ttggctgcgg tagtagccgt cgcgttcgcg
    10501 cgcttcttcg agttcttgca gggcggcgag gttgaccgcg ccgagtgctt cgatttgttg
    10561 cgaaaggctg ccgatgctgc tgttcaatac tttcggcgat tctttcgcca acgcttcgag
    10621 cgcgtccaaa tcggcggcgc gttcggtcag gttttgatgg tagcgtttgg cgttgatcag
    10681 ggcttcctgc tgctgcaaca aggcggtttg ggtggcggcc tgaagctgcg gcagcttggt
    10741 ttgcagggtt tgcactttag cgtattgctc cctgccctgt tcctgaatct gcgcgagttt
    10801 ctcttgcaca acaatatatt cttcgtccaa ggtctgtacg gcttcgctta attcttcaag
    10861 cttgatgtgc tgctcgtcgt tttggaactc ggtttcatag gcgagggcaa gctcttgctg
    10921 gcgttcctgc cagtcgaggg tttgctgttc aagctgggcg atttgctgcc ggtagttttg
    10981 tttttgctgg ttgagtttgt ggacggcgac ttcggcaagc ccatattggc ggttggcttc
    11041 caacagggca agctgcgcct gtttcagacg gccttgctgc tcttggcggc tgtgcgcggt
    11101 ggtttgctgc tggtgttcga gttcggcggc ggcttcctgc aaggtaacga tgtcgtctga
    11161 aagcccgtcg gacgtgtgtt gcaacacggt ctgttcttcc gccaactgcg ccagttcgcg
    11221 ctcgatgtgt tcgcggcgga tttgcccttg gttggtacgc gctaagagtt cggcggcgcg
    11281 ttgctgcgcc tgactgtatt ggcgcgtgtg ctgctgttgc tgctgcatca ggtttttatg
    11341 ttgcacttca gacgagcgca cggcagcttc cgcctgtttg aacgcggctt cggcggcgga
    11401 aagttcgggg gcgaggtttt ccagttcgga cgcgatgccg tcgaggcgcg ctttttgggc
    11461 aatcaggctt tcctgcgcgg gtttggcata gagcaggacg ctgactttat cgacctgatg
    11521 accttcgggc gtgagccaga tttggtgtgc gcccaaatcg ttttgatgcg cgagggcata
    11581 gctcaaatcg ggcgcgcaca atacgccgtc gagccagtag tgcaatgccg tctgaaacgg
    11641 cggctgcgct tggatttggt tcagcaatgc ctgtacgggc agggattttt tgatgccgcc
    11701 ggagagaccg tctgaaagcc acgccgcctg cccctgcggc aaaggttcgg gcggtacgaa
    11761 accttgcggc acggcgcggg cgtgcaggcg ttctgcaagg atgacggaca aggcgtgctg
    11821 ccactcggca ggcgcggtga tgtgttgcca cagctgcggc gcggcggcgt ggtcggttgc
    11881 ctgccagaag tcggcggctt cctgctgttg cgacaggatt tgcgacaacg cctgctgctg
    11941 cgcctgcaag gtgatgtgtt gctgcttcag gctttggaag cggtttgatg ccgtctgaaa
    12001 cgcctcgcgg gcggcgtgta gagcttcttc ggcggcgatg atttgttctt cgtaatgctc
    12061 ttgctggctt tgcaacaagg cggcggcttc ctgcgcggcg gcggtttcgg cttcgtcggg
    12121 caggttcagg gcttggtttt cctgtttcag acgacctttg cgctcttcgt gcttggcaac
    12181 ggtttgttcg gcatgggcaa gctgctgctg cttcaacgcc agttcgcggc ggatgcggtt
    12241 tgcctcgtcc tgctgggttt ggaaggcggc attgagcgtg gcttgggctt cttccaattc
    12301 gggcagacgc tcttcgtgtt cggcaacctg catcgcccat tccgccagct cggtttgttt
    12361 ttcttcgacc tgcaactcgt tttcttcaag ctgcacgcgg atttgctgct gctcttgatg
    12421 aatgcgttgt aactgcgcct gcgccgcctg cttgtcgcgt tcgatgcgtt gatgcaggtt
    12481 ttgctgatgg cggatttgtt cttccaaacg ggcaatctgc tcgcgcaaca cgccgcgttt
    12541 gttgcccagt tcgtgtaccg cctgctgctg cgactgttcg gcggtctgca aggcgtgtac
    12601 ttcgtcgttt aacgcctgaa cctgcgcggc ggtttcgtcc tgctgcgcct gcaaagactg
    12661 atgctgcgcg gtcgctttgt cggcggcggc aagcgattgc tgccattggg cgtaatcgag
    12721 caaatcctgt tgttgattca actgcgcggt cagggattta tagcgttcgg cggtttccgc
    12781 ctgtttctcc agcttttcca cctgacgcgc caattcgttc tgcaaatcgc ccaaacgctg
    12841 caaatgctcg cgcgtgtctt tcagacagcc ttccgtctcc ttgcggcgtt ccttatattt
    12901 ggacacaccc gccgcctcct cgatataagc gcgcaactcc tccggccgcg cttcgatgat
    12961 gcgcgaaatc atcccctgct cgataacggc ataaccgcgc gcgcccacgc ccgtacccag
    13021 aaacaaatca gtaatatcgc ggcggcgcac ggtctgattg ttgatgaaat aagtcgattc
    13081 gccctgccgc gtcagctgcc gcttgatgct cacctcggca tactgccccc acgcgccctg
    13141 caaactgtgg ccgctgttgt caaacaccag ctccaccgaa gctcttggcg caggacggcg
    13201 cgttgccgca ccgttaaaaa tcacgtcctg catactctcg ccgcgaagct gcttcgccga
    13261 agcctcaccc aacacccagc gcaccgcgtc aatcacattc gacttgccgc agccgttcgg
    13321 cccgataacc gcgacaagct gccccggcac atgaatcgtg gtcgggtcgg taaaagattt
    13381 gaagccggag agtttgatat gggtcaggcg cataatggtc ggaaaaaata aaaaagaagc
    13441 gtattttaac ggaaatcccg ccgcaccacc ccatatcttg ccggcaaaac cttaccgcat
    13501 cccgcaccct cgatgccgtc tgaagccttc agacggcatt tttaacgcgc ccgaaccccg
    13561 attccgccaa cgcccgatgc ttggcgcggc atttgccgta ttgccccgcc gccgggcgcg
    13621 ttataatccg ccacgacatc cgcccgattt ggaaacctgc ctatgaaacc cgcctatttc
    13681 atttccgacc tgcatttgag cgaaaagcag cccgaactga ccgcgctgtt gctgcgtttt
    13741 ttacgttctt ccgccgccag gcaggcgcgg gcggtttaca ttttgggcga tttgtttgat
    13801 ttttgggtgg gcgatgacga agtttccgag ttgaatactt cggttgcgcg tgaaatcagg
    13861 aaattgtccg acaaaggcgt tgccgtgttc ttcgtcaggg gcaaccgcga cttcctgatc
    13921 ggtcagaatt tttgccggca ggcgggcatg acgctgctgc cggattactc ggttttggac
    13981 ttgttcggct gcaaaaccct catctgccac ggcgacactc tgtgtacaga tgacagggca
    14041 taccaacgtt tccgcaaaat cgtgcatcgg aagcggctgc aaaaactgtt cctaatgctg
    14101 cccctgaagt ggcgcacgcg ccttgccacc aaaatcaggc gtgtcagcaa aatggaaaaa
    14161 caggtcaagc ccgccgatat tatggatgtc aatgccgcct ttaccgcgcg gcaggtacgc
    14221 gccttcggtg cggaaaggct gatacacgga cacacccacc gcgagcatat ccatcacgaa
    14281 aacggcttta cccgcatcgt tttgggcgac tggcataacg actatgcttc aatcctccgc
    14341 gtggacgggg acggcgcggt attcgtgccg ctggaaaaat actgaaaatg ccgtctgaag
    14401 cctttcgggc ggcttttttt acacgcccct ttcccacact ccctccgccg catcatcact
    14461 atccttaacc cgcattaata gccattatcg ataaaatgct tgaccgatta cataagatta
    14521 cgtaaagtgt gcaaacgcat aacaattggt cttaccaatt tatcttttaa ccaaaatatc
    14581 gggtgtacgg gatttgaacg tctttccaaa cctcgacgcg ccttatgatg caaatgccta
    14641 aaaaggattc gttatggcac ttttcctcag catattcccc atcgtcctgc tgatttggct
    14701 gatggtgaaa aaaaacagta tgccctccta cgtcgcgctg ccgattaccg cagtgctgat
    14761 ttacgccatc aaacttttct acttcgacga tgcgggcatg ctgctcaacg ccaccgccgc
    14821 ttccggcctc gtcaaaacgc tcacgccgat taccgtgatt ttcggcgcga ttatgttcaa
    14881 ccgtatgatg gaaaccacgg gctgcatcga tgtcatccgc aaatggctgg cgaccatcag
    14941 cccgaacccc gtagcgcaac tgatgattat cggctgggct tttgccttta tgattgaagg
    15001 cgcatccggc ttcggtacgc ctgccgcgat tgccgcgccg attctgatga gcttgggctt
    15061 caacccgttg aaagtggcga ttttcacttt ggtgatgaac tccgtccccg tctctttcgg
    15121 cgcggtcggt acgccgacct ggttcggttt cgcaccgctg aacctgagtg ccgaagacat
    15181 cctcgccatc ggcaggcaga ccggcgtaat gcacttcttc gcaggtttcg tcatccccgt
    15241 catcggcttg ggcttcatcg taccttggtc tgaaatccgc aaaaacttgg gcttcgtcgc
    15301 cattgccgtc ttctcctgca ccattcctta tgtcgcattg gcgatggtca acgaagaatt
    15361 cccgtcgctc gtcgccggcg cgatcggcct gatggtgtcc gtattcgccg ccaaccaagg
    15421 ctggggcttg agcaaagacc acgccaaaga cccgaatgcc gaaaaagtgc cgttcgccca
    15481 agtcgccaaa gcactcgccc ctttgggtat gctgatcggc atgctggtgg ttacgcgcat
    15541 caaacagctc ggcatcaaag gcattttgac cagcaaagaa gaatggttca gcttccaact
    15601 gccgtttgat ttgtccaaaa tcaccgtcag cgactccctg acgattacct tcggcaatat
    15661 tttcggacaa gatgtcagcg cgtcttacca aacgctgtac gtcccggctt ggattccgtt
    15721 tgtgctgacc gtttggattt gcatcctgct gtataaaacc aaattcaaag atgcctggac
    15781 gatttattcc gtaaccttca atcaaaccaa aaaaccgctg cttgccctga tgggcgcgct
    15841 gattatggtt cagctgatgc tggtcggcgg cgacaattcg atggtgaaaa tcatcggtaa
    15901 ggaatttgcc gcaatggcgg gcgaacactg ggtttatttc tcgccgtatc tgggcgcgat
    15961 cggtgcgttc ttctccggtt ccaacaccgt gtccaacctg accttcggcc cgattcagca
    16021 gcaaatcgcc ttggataccg gcctgtccgt taccctgatt ctggcgttgc agtccgtcgg
    16081 cggcgcgatg ggcaatatgg tgtgcctcaa caacatcatc gccgtatgta ccgtattgga
    16141 tgtgaaaaat tccgaaggtg cgattatcaa gaaaaccgtt atcccgatgg cgatttacgg
    16201 cgtgattgcc gtcgtcgcgg caatgatttt cttcctctag gcgaaacgcc accatccata
    16261 gcggcagcac gcggggaacg gaaaccgccg ttcaccgtct gggcggcgcg tcgggcagaa
    16321 tcccggtgcc ggaacaagcc cgccccgcca aacaaatgcc gtctgaaacc gaaaaaggct
    16381 tcagacggca tttttccgcc gtttgcgttc aggcgtaatg atgatgcggc atcttgccgt
    16441 caaggcggta gcgcgtgccg caatacgggc aggcaacgct gcccgattct ccttcgcaca
    16501 aaggcaaaaa cactctcgga tgcccgttcc actgctcgtt gccgggtccc gagcaataca
    16561 gcggcagatt ttccggcaac acggaaattt cctgcggatt cagattgtcc atttgatttt
    16621 cctttgcgtg gttggcgtgc gcctatttta cgccatcggc aggctaaagg atattttcgg
    16681 cgcaaagccg caatccgcta taatccccac ttttcagacg gcataccatg actgcgctta
    16741 cccttccgga agacatccgc caacaagagc catccgccct gctctatacc ctcgtttccg
    16801 cctacctcga acacaccgcc caaaccggcg acgaatccct ctcctgcctg tccgacgacc
    16861 agcacacgct gaccgcattc tgctacctcg acagccaagt cgaagaaggc ggcttcgtgc
    16921 aactcatcgc atccggttac ggcgaatata ttttccgcaa cccgcttgcc gacagcctgc
    16981 gccgctggaa aatcaaagcc gtgccgaaag tcttggacaa agccaaagcc ctctacgaac
    17041 aacacggcaa aaccatcgaa acgctcgccg acggaggcgc agacatccct tccctgcgca
    17101 aacagttccc cgaatttgaa gaatgggacg gcgcatacta cgaagccgcc gaacaagacc
    17161 tgcccctgct tgcagaacac atacagtcaa actgggaaac cttcgcccat atcgggcagg
    17221 cgtgattgcg tctgtttcca gcccgtgtaa aacagcgtaa aatcggcaaa cccgaatgac
    17281 cttccgtccc catcaaggag caagctatgt tcttcaagca catcgaagcc gcccccgccg
    17341 atccgattct cggtttgggc gaagcgttca aagccgaaac ccgccccgaa aaagtcaacc
    17401 tcggcatcgg cgtttataaa gacgcatccg gcgcgacacc cctcgtcaaa gccgtcaaag
    17461 aagccgaaaa acgcctgttg gaaagcgaaa ccaccaaaaa ctacctcacc atcgacggcg
    17521 ttgccgacta caacgcgcaa acccaaatcc tgctgttcgg caaagaccac gaaatcatcg
    17581 ccagccgtcg cgccaaaaca gcgcaaagcc tcggcggtac gggcgcattg cgtattgcgg
    17641 ccgagtttgc caaacgccag ttgaacgcgc aaaccatctg gatttccaat ccgacttggc
    17701 ccaaccacaa cgccatcgcc aaagcggtcg gtatccaaga caaaccttat cgttactatg
    17761 atgccgccaa acacggtttg gattgggacg gcatgattga ggacttgagc caagcgcaaa
    17821 aaggcgacat cgtcctgctg cacggctgct gccacaatcc taccggcatc gaccctacgc
    17881 ccgaacaatg ggaaactttg gcaaaacttt ctgccgaaaa aggctggttg ccgctgtttg
    17941 actttgccta ccaaggcttc ggcaatggtt tggaagaaga tgcctacggc ttgcgcgtgt
    18001 tcttgaaaca caatacagaa ttgctgattg ccagctctta ttccaaaaac ttcggtatgt
    18061 acaacgagcg cgtcggcgcg ttcactttgg tggccgaaga tgaagaaaca gcagcccgcg
    18121 cccacagcca agtcaaaacc atcatccgta ccttgtattc caacccggct tcacacggtg
    18181 cgaacaccat tgcgctggtg ttgaaaaatg atgatttgaa agcacaatgg attgccgaac
    18241 tcgatgaaat gcgcggccgc atcaaagcca tgcgccaaaa atttgtcggg ttgctcaaag
    18301 ccaaaggtgc aagccaaaac tttgatttca ttatcaaaca aaacggtatg ttctctttca
    18361 gcggcttgac tcccgaacaa gtcgaccgcc tgaaaaacga gtttgccatt tatgccgtcc
    18421 gctccggccg catcaacgtc gccggcatta ccgacaacaa catcgattat ctgtgcgaaa
    18481 gcatcgtgaa agtactgtaa aaagcacagc catatgaaaa tgccgtccga accttaacca
    18541 gttcagacgg catctgtttt ttgttaaaaa acaataaacc ataacaattt caaacaagct
    18601 aaaaacatct ataaaatcaa ccaaatatga tgaactcaaa taaaaaatac gaatatcatc
    18661 attctcaaat tcaaaatttg aacgccacag tattatccga agccacaaaa acctttccgc
    18721 cgtcattccc gcgcaggcgg gaatccggga atccaatgct gcaagaattg attagaaaca
    18781 actgaaaccg agcaaactgg attcccgcct gcgcgggaat gacgaaataa ggcgagtgaa
    18841 actgatgaaa caaattgatt aaaaacaata acttatatta ttttatttaa attttcgata
    18901 aaatcaattt tccgtattca atactgctcc aatcaattcc cgcgcaccgt cgtccgccaa
    18961 tttcttagcg acagcgcgtc cgagcgcgtc ggcatattcg gcaggggctt gcgcgtccgc
    19021 ccgcaacaca accgaaccgt cggggtgtcc gaccaagccg cgcaaggtca gcaagccgtt
    19081 ttcttccgtg caatatgcgg ccaaaggcac ttggcagctt ccgcccaaag cgcgtgcgag
    19141 ggcgcgttcg gcggtaacgc aggcattggt aacaccgtgg ttcaagggtt tcaaaacttc
    19201 atacaaatct tcgcggtgcg cggcaatttc gatacccaat gcgccttgtc cggcggcagg
    19261 caggctgtcg gattccgaca aaatcatgcg gatgcgtccg tccaatttca gacgctgcaa
    19321 accggcggca gccaagataa ttgcgtcgta ttcgccgtta tcgagtttgg acaaacgggt
    19381 ttgcacattg ccgcgcaaag gtttgataag caaatgcgga tagcgcgcac gcaactgggc
    19441 ttcgcggcgc aggctggatg tgccgacaac cgcgccttcg ggcatttctt ccaaacgcgt
    19501 gtattggttg gacacaaacg cgtcaaacgg attggcgcgt tcgccgatgg cggcaagcgc
    19561 gaaaccttca ggcaaatcca tcggcacgtc cttaatcgaa tgcaccgcca aatcggcgcg
    19621 cccgtcatat aaagcctgtt ccaactcttt gacaaacaag cctttaccgc cgacttttga
    19681 caaagttttg tccaaaatct gatcgccgcg cgtggtcatg cccaaaatct cgacttcgca
    19741 atcgggatac agagccttca gacggccttg gatatgcttt gcctgccaca tagcaagcag
    19801 gctttcgcgg ctggcgatga cgagtttttt cgggttcatg ttcaatgcgt tcaaagaatg
    19861 gaaacggcgt gaagtctatc atattttgaa cgtttcttca gacggcaccg tcaggtctat
    19921 gttatattta cacccacaaa acacgaaacc cgccacaatg aaacgcacca aaaaataccc
    19981 tttcttaacg ttgcagcggc aacgtttcca tttgaacttt gaaaacgcct cttccgccgc
    20041 cggcatccca gccgaacgcg atttctaccg ctgggcgtgg tctgccttga aaaatgaata
    20101 ccgccgcgcc gacatcagcc tgattcttct ggacgaagaa gaagcccgag cctacaaccg
    20161 cgactaccgc ggcaaagatt acgccaccaa tgtattgagt ttcgcgctca acgaaggcga
    20221 aatcctgccc tgccaagttt cggaaaaact gtacggcgat ttgattatct gcccgcaagt
    20281 ggttttgaaa gaagccgccg aacaaggcaa aacacccgag cagcattttg cccacctgac
    20341 catacacggt actttacacc tgatgggcta cgaccacatc gaagacgacg aagccgaaat
    20401 aatggaagcc gaagaaatcc gcctgatgcg ggcggcaggc ttccccaacc cctaccaaga
    20461 ggactgacat taaaatggac ggcgcacaac cgaaaacgaa tttttttgaa cgcctgattg
    20521 cccgactcgc ccgcgaaccc gattccgccg aagacgtatt aaacctgctt cggcaggcgc
    20581 acgagcagga agtttttgat gcggatacgc ttttaagatt ggaaaaagtc ctcgattttt
    20641 ccgatttgga agtgcgcgac gcgatgatta cgcgcagccg tatgaacgtt ttaaaagaaa
    20701 acgacagcat cgagcgcatc accgcctacg ttatcgatac cgcccattcg cgcttccccg
    20761 tcatcggcga agacaaagac gaagttttgg gcattttgca ctccaaagac ctgctcaaat
    20821 atatgtttaa ccccgagcag ttccacctca aatccattct ccgccccgcc gtcttcgtcc
    20881 ccgaaggcaa atcgctgacc gcccttttaa aagagttccg cgaacagcgc aaccatatgg
    20941 cgattgtcat cgacgaatac ggcggcacat ccggcttggt cacctttgaa gacatcatcg
    21001 agcaaatcgt cggcgaaatc gaagacgagt ttgacgaaga cgatagcgcc gacaatatcc
    21061 atgccgtttc ttccgaacgc tggcgcatcc atgcagctac cgaaatcgaa gacatcaaca
    21121 ccttcttcgg cacggaatac agcagcgaag aagccgacac cattggcggc ctggtcattc
    21181 aagagttggg acatctgccc gtgcgcggcg aaaaagtcct tatcggcggt ttgcagttca
    21241 ccgtcgcacg cgccgacaac cgccgcctgc atacgctgat ggcgacccgc gtgaagtaag
    21301 caccgccgtt tctgcacagt ttaggatgac ggtacgggcg ttttctgttt caatccgccc
    21361 catccgccaa acataaaaat gccgtctgaa accgttgcaa gtttcagacg gcatttcgct
    21421 tatccgctta ttttttgctc aaagtcagcc ccgtccaacc gaataacagg gtcaaagcca
    21481 aactcaaata gcagaaaaag gcatacggca gatattccca aaccggcacg cccagcgcgt
    21541 ggctgatgaa cacgccgcat acgctccacg gtacgagcgg gttaatcacc gtccccgcat
    21601 cttccagcgt ccgcgacaga ttgcgcgaat gcagaccgag cttatcgtaa acgggtttga
    21661 acgtttcacc cgacaacaaa atactcaaat attgctcgcc gatcaggaaa ttaaccccga
    21721 ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag gtacggatgg
    21781 cctccaacag ggaaggaatc acaccgagcg caaacaacag tccgcccaaa ctcatcccga
    21841 gaatcacgat ggtttgcgtg aaaaacatac tttccaaacc gccgcgcgaa atcagtttga
    21901 caacatcttt aaacgcttcg ccttcgagtt tgtagccgcc gtaaaaccac gcaccgagct
    21961 gacgcagatc gggcgtgctg tgcagatacg ttacagcaac ggcaaccatg acggtaaaga
    22021 gcatggcgac gacggcgttg atgcgcatca atgccaaaat gaccaacagc gcaaacggaa
    22081 tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag gattcgacgc
    22141 tgttcaaatc ctgcgcggcg acattcggca aaagccaaag catcagtgcc gcactaatga
    22201 gccacgcggg gatggtggtg tacatcatat ttttgatgtg ctcaaacaag tcgatgccga
    22261 cgatggacgc ggaaataccc gtcgtatccg aaagcgggga cattttgtcg ccaaaaaatg
    22321 cgcccgaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc gccgccatcc
    22381 ccataaaggc aacgccgaca gtggcgcagg tggtcaggct gctgccgatg gacacgccga
    22441 tgacggaaca cagcgcgaag gaggaaaaat aaaaataagt cggggaaatc agtccgaaac
    22501 cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc atcagcccga
    22561 tgaagaaaaa caggtaaatc gcgcccatac cctgattcaa cgcgcctatc atgccctgct
    22621 gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg acgatggcgg
    22681 caataatgga catatgcggc aaccactcca atgaaatgat ggtatagccc atcgcggcaa
    22741 tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag gatttgaaag
    22801 cgaacatgat tctttgcccg atttgatata aagttaacgg attctaacac accataccga
    22861 atccgcccga cgttttcaga cggcatttta gcgttttaca aaagccggac agaaacaaaa
    22921 cggcaatttt ctcaatttaa attaattttt ttgaaatata attttttatt tttttgtttg
    22981 ttgacatata agacttttat ggattgaagt attaaaacct cggcacacac gccgcaaaca
    23041 ccggtacgca aaaatgccgt ctgaaagatg ttcagacgac atttctcaag aaaaagatta
    23101 tttgtccgcc ctatatccgt acaccgcaaa aaacacgata tacagataac agatgacgga
    23161 aacgacaaac gaagacatca gggtgtaagt atctgccacc cagccctgca cgacaggaac
    23221 gaccgcaccg ccgacaatcg cggtacacag tacaccggaa gccgcgttgg taaattttcc
    23281 caatcctttg gttgccaaag agaaaatcgt cggaaacata atcgagttga aaaaaccgat
    23341 ggcaagcagc gaccacatcg ccacatccgc attgccgcta cccgtcgcca tcgcgacggc
    23401 aagcagtacg accgcagccg atgcgttaaa cgccaaataa cggttgggcg cgaatttcgc
    23461 catcaccgcc gaaccgagga aacgtccgac catcgcgccg ccccaataga acgacaggta
    23521 atgcgcggca gaagcatgat ccagcccttt cagataaccc aatacgttga ccatcaacga
    23581 accgatagac acctccgcgc cgacatagca gaaaatgcct gccgtaccga acacgagatg
    23641 tttgtattgc catacgctgg ttttgccgtc gtggttgtgc gcgctttcct cggcggcaat
    23701 tttgcgcgcg tcgggcagcc ggatcatttt cacgaaaacg gcaaggataa tcagcagccc
    23761 cgccagtccc aaatagggaa tctgtacgga agaaatctgt tccgccttgc tgacggtttg
    23821 ggttgcgtcc gccagaatca ggaacgcgcc gatttgcggc gcaatggtcg tacccaaagc
    23881 gttaaacgcc tgaaccagcg tcagtgttgc cgattccttg ccgggtttcg ccagcagggt
    23941 aacataagga ttaccggcga cctgaagcag cgttacgccg gaagccaaaa taaacaacgc
    24001 gcccaaaaat accgcgtaag aatggctgcc cgcagcagga taaaacagca ggcatccgac
    24061 cgccgtcagc agaaagccgc cgataacgcc gtttttgtag ccgactttgc cgacaaaagc
    24121 ccccatcggg atggacatca ccgcataggc ggtaaagaaa cagaattgga tcagcatcgc
    24181 ctgaacgtaa gacaggtcga aaatttcttt caaatgaggg ataaggatgt cgttcatgca
    24241 ggtaataaaa cccatcatga agaacagcgt ggtcaagacg accaatgggg acgtatgatt
    24301 gttttgtgat tgcgcagaca tgatttgcct ttcgtggtgt cggaatatta cccgtttcaa
    24361 gaaaatagaa attgacattt cttaacggca atgcaaatca tactgatatt tgaacatttt
    24421 gcaaacttta tcggatgcag gcaatgcgcc gatctgaaat tcaagcagca gaaattaata
    24481 ttttacagtt gcttgcatag attttaatag attttaaaaa attccggcgg acttgcaaaa
    24541 accgttccca ataaaatgaa aatccgccgc cgtatccgga aaattcggaa atgccgcatc
    24601 gtggcatcaa aaccggagcg gaaagaatgt cgtatgcccg acaaaaaagg ggcatacggc
    24661 tgtgccgtat tttttgcacc gtcctccgcc cttcggcggg ggcgtgaaaa ta
//
LOCUS       DAWISC010000010        24328 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWISC010000010 DAWISC010000000
VERSION     DAWISC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.010"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(431..2272)
                     /locus_tag="WIW87_000290"
     CDS             complement(431..2272)
                     /locus_tag="WIW87_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ5173509.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDIFGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2363..2833
                     /locus_tag="WIW87_000291"
     CDS             2363..2833
                     /locus_tag="WIW87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ5173510.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(2906..3718)
                     /gene="hpnC"
                     /locus_tag="WIW87_000292"
     CDS             complement(2906..3718)
                     /gene="hpnC"
                     /locus_tag="WIW87_000292"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ5173511.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3805..4287)
                     /locus_tag="WIW87_000293"
     CDS             complement(3805..4287)
                     /locus_tag="WIW87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5173512.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4760..5056
                     /locus_tag="WIW87_000294"
     CDS             4760..5056
                     /locus_tag="WIW87_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173513.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            5166..6533
                     /locus_tag="WIW87_000295"
     CDS             5166..6533
                     /locus_tag="WIW87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ5173514.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(6714..8696)
                     /gene="autB"
                     /locus_tag="WIW87_000296"
     CDS             complement(6714..8696)
                     /gene="autB"
                     /locus_tag="WIW87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter AutB"
                     /protein_id="HEZ5173515.1"
                     /translation="MVSLFFSILYTSPLLAVDYVYDKTKLTNDEITRLKKLRDKTSEY
                     WKKETYLITEDNPKVPPFPALYPRTYQFENINNSKKISFYDQEYTEGYLVGFARGLGV
                     AKRNGDTEEQIRKYFKECFNSNTKIRDYSTCQAEKFGSHPLIVKSHIFSLGPKIKNSH
                     INSEILSVGNYTEWANQVIHHIENYVSFAAHLYSGLDPFHYIEVTDNSHVIGQTISLD
                     EFRLENSLWEPRWDSNVGKLKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDPKEAK
                     NTALIFEKNVTGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYTL
                     SLQQRPEGFFTKVQERDDMAIYAQQAQAANTLFALRLNNKNSDIFDRTLPRKGLWLRV
                     IDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFHNPD
                     TDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWMQYQRFRHRINTEDGTERFTSKG
                     ITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSRQ
                     LQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDGERRVINNKTAIESQLG
                     VAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(9115..9561)
                     /gene="smpB"
                     /locus_tag="WIW87_000297"
     CDS             complement(9115..9561)
                     /gene="smpB"
                     /locus_tag="WIW87_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ5173516.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPNAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(9616..10626)
                     /gene="waaF"
                     /locus_tag="WIW87_000298"
     CDS             complement(9616..10626)
                     /gene="waaF"
                     /locus_tag="WIW87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ5173517.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(10757..10832)
                     /locus_tag="WIW87_000299"
     tRNA            complement(10757..10832)
                     /locus_tag="WIW87_000299"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10797..10799),aa:Asn,seq:gtt)
     gene            complement(11024..11776)
                     /locus_tag="WIW87_000300"
     CDS             complement(11024..11776)
                     /locus_tag="WIW87_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ5173518.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            12077..12892
                     /locus_tag="WIW87_000301"
     CDS             12077..12892
                     /locus_tag="WIW87_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine-binding family protein"
                     /protein_id="HEZ5173519.1"
                     /translation="MTAYNESTYSSSKGIIYANPHPLRRTAGFFNRCLCRGRIHAAIR
                     QPVRRRRLHAKPAFERALRLLLFRRQCFARAVVEKSARRDKSFVLTVYDKDAPTGLGW
                     MHRVVADIPADVHRRNATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKP
                     LRQHRRRPVRSHIGGNQFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRSIVRHFFI
                     RQYRSLIIKRRAHTPGIRQLYRIAHCLRRPVLCGEPFYGFLFESNPLRLIEAV"
     gene            13079..13189
                     /locus_tag="WIW87_000302"
                     /pseudo
     CDS             13079..13189
                     /locus_tag="WIW87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13563..14708)
                     /gene="dapE"
                     /locus_tag="WIW87_000303"
     CDS             complement(13563..14708)
                     /gene="dapE"
                     /locus_tag="WIW87_000303"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ5173520.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAKHPNHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAQELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(14821..15441)
                     /locus_tag="WIW87_000304"
     CDS             complement(14821..15441)
                     /locus_tag="WIW87_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ5173521.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(15475..15984)
                     /locus_tag="WIW87_000305"
     CDS             complement(15475..15984)
                     /locus_tag="WIW87_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5173522.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(16079..16630)
                     /gene="azu"
                     /locus_tag="WIW87_000306"
     CDS             complement(16079..16630)
                     /gene="azu"
                     /locus_tag="WIW87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ5173523.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(16911..17333)
                     /locus_tag="WIW87_000307"
     CDS             complement(16911..17333)
                     /locus_tag="WIW87_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ5173524.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            17464..20214
                     /gene="secA"
                     /locus_tag="WIW87_000308"
     CDS             17464..20214
                     /gene="secA"
                     /locus_tag="WIW87_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ5173525.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSKLLTQAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            20229..20381
                     /locus_tag="WIW87_000309"
     CDS             20229..20381
                     /locus_tag="WIW87_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173526.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            20359..22131
                     /locus_tag="WIW87_000310"
     CDS             20359..22131
                     /locus_tag="WIW87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ5173527.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLTQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            22318..24246
                     /gene="rpoD"
                     /locus_tag="WIW87_000311"
     CDS             22318..24246
                     /gene="rpoD"
                     /locus_tag="WIW87_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ5173528.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         6320 a         6743 c         5647 g         5618 t
ORIGIN      
        1 aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
       61 atccactata tccgactttc catacttaaa cagcactgcc tcttttcaga cattgacggt
      121 tgcagccgcc cacctgaacc ttaatgattg actatttttt aatcatgtta ttattttcca
      181 taaaatacat gacattaaga tgtttttcca caaaagatac acacaccggc aaacaccggc
      241 tgtgtttatc ttttcttatg cctatttttt aatcatcgta tttttatctt ttaatttcaa
      301 tacgcaaact aacttataca cacggttttc acatctttag actgcttccg tgtgtatagt
      361 ggatattgcc gttttccttt ctgacaaaaa tgccgtctga gaacttcaga cggcatttga
      421 aacatcggaa tcagcggttt tgttcatacc actcgataaa cttgtctgct ttgacaaaac
      481 ccagcagcgg ctcgctgcgg ctgccgtcgg agcggacgac aaacacgccc ggcggcccga
      541 acagaccgta ttctttcaac aacgcctgat gttcgggcgt gttggcggtt acgtcgattt
      601 ggaaaaagcg ttccatatcg actgcctgat gcacttccgg ctgattgagc gtgtaagccg
      661 ccatttcttt gcaggaaatg caccagtcgg cataaaaatc caaaacgacg ggtttgtcgg
      721 gatgttcttt caacgccgta tccatcgctg ccttcagcgc ggcagtatcg gcaaacattt
      781 tgccgtgttc cgaagatttg cctgcttcgg ctggtggatt gagggtcagg aaatggtgca
      841 gcgcggtcgt tttgccgttt gcgccctgcc agccgaacca cgcgccgcct atcagcaata
      901 taccgcccaa tgcgaatgcc acagctttcg gacggcgttt ctgcctgcgt ccgttgacca
      961 gcagcataaa ggcaggaacc agcatcagca gcgtgtacag cgcgacgacg agataatagg
     1021 gcaagtgcgg cgtggcgagg taaacggcga cggctagcag gatgaagccg aatgcgtatt
     1081 tgacggcatt catccaatcg cctgccttag gcaggatatg cccgccgaac gtgccgatgg
     1141 caatcagcgg aacgccggtg cccaacgcca aagtgtaaag tgccaaaccg cctaaaaccg
     1201 catcgcccgt ctgaccgatg tagcccaaag caaatgccag cggcggggcg acgcacggcc
     1261 cgacaatcag cgcggacaat atgcccataa taaagacgga aacgatttta ccgcctgaaa
     1321 gcctgctgct ttgattctga aaatacgact gcacggcgtt gggaagctgg atgttgaaca
     1381 gcccgaacat agacagtgcc aagacgacca ttaaagccga tgccgccaat accacccaag
     1441 cctgctgcaa ccatacggtc agcagtgcgc ccgtcagtcc ggcaacaatg ccgaccagcg
     1501 tataagtcag agccaaaccc tgaacataaa cgacggacag cacaaacgcc cgcgccttgc
     1561 ccgccttttt gtcgccgacc acaatactgg aaacaatcgg caacagggga tacatacagg
     1621 cggtaaaact caggcccaaa ccagcgagaa aaaacgccaa aagattggcg ttgagcgtat
     1681 cccaagacag cttgaaacgg ctgtcgccgc cctcatcccc cttcgggggc ggcagcgccc
     1741 cgctgccgtt ttgagaggaa ggctgcaaaa agcggtcttt ggcggatgcc ggttcgtcgg
     1801 tttgcggatg gtaagtgccg ttgccgaaaa tatcaaactc ggtatccacg ggcggatagc
     1861 acacgccggc ttcggcacag ccctgatagg tcaaaaccaa tttatacggt tcgccgacag
     1921 cctttgcata aggaaaggca acctgcgcct cgtgatggta aaccgtctgc ctgccgaaaa
     1981 actcgtcttc cttctcttcg cccttgctga aagaaggctg tcccaacaaa tccgccggat
     2041 cggtcttgcc gacgattttc gcctgataca tatagtatcc gtcggcaatc ctgaaacgga
     2101 cgttcacacc gtcgtcggca acggcaagct ccggcacgaa tgccttttcc ggcggcagca
     2161 gatcgttcgc atccagcgcg aaagctcgtc cgcacaacat caaaaatacg gcgaacaggc
     2221 aaatcagttt tttcataatc gaatccgttt cagacaaata atttgtctgc attataaatg
     2281 gtaaggttga cggtgggatt taatttatgt aaaacccgcc attatccgaa cctatttcca
     2341 taaacatctt atcgaacccg ccatgtacga tgtcaatacc cacgatgtcc gccgcttttt
     2401 cgcccgcgtg tggcagcagc ggctcaatcc gctgcaactg agcgcactgg aacagaaagc
     2461 cctccgcatt gtcgaagccc atcccgaata ccaccgttat ctcgaacgca tcgaagacca
     2521 tctggacacc gactggctgc ccgaaaacgg cgaaagcaac cccttcctgc atatgtcgct
     2581 gcatctgtcc gtccaagaac aggcgggcat agaccagccg cacggcatac gcgcaatcca
     2641 cgacaccctg tgcgccaaac gcggctggct ggaagccgaa cacgaaatga tggaggcact
     2701 ggcggaaaca ctgtggacgg cgcaacgcta cggcaccggt ttggatgtca atttctacat
     2761 gacccgactg cgcaaactca tcggcttggg tgcagaagat caagccagat tgaacccgca
     2821 tgaaatcgcc tgaccatacc aaccgcctgc aaaatgccgt ctgaagcgga acaacccctt
     2881 tcagacggca ttcattttcc cccaatcatt tccacaacgc ctttttcagc ataatcaacc
     2941 aatccttctt atccaaaacg gggcgttgtg caaacacatc gtatcggcac gcgtccagtt
     3001 tctgcaaaat caactgcgcc cccaacacaa tcatacggag ttccaaaccg atacgcccat
     3061 tcagttccct tgccaaaggc gaacccgcct tcagcatacg gaacgcacgc cgacactcat
     3121 acgccatcag ccgctgaaac gccgcatccg cccgtcctgc cgcgatctgt tcctcagaaa
     3181 caccgaattt caacaaatcg tcctgcggaa tataaaccct gcctttttgc caatccacag
     3241 ccacatcctg ccaaaaattc accagttgca aagccgtaca gatgccgtcg ctttgcgcca
     3301 cgcacaccgc atccgttttc ccgtacaaag ccagcataat gcgtccgaca gggttggcgg
     3361 aacgccgaca ataatcggcc agctcgccga aatttccata ccttgtttta accacatcct
     3421 gagaaaatgc agaaagcaaa tcataaaacg gctgcaaatc caaaccgaac ggcacaaccg
     3481 cctcggcatc caatcgtgca atcaaaggat gcgccgaccg gccgcccgat gccaacacgt
     3541 ccaactcgcg ctgcaaaccc tccaaccccg ccaacctggc ttcagacggc atactgccct
     3601 cgtccgccat atcgtccgcc gtccgtgcaa acgcgtacac cgcgtgaacc ggcttcctca
     3661 acctgcgcgg caaaatcagc gaaccgacgg gaaaattctc ataatgccca accgacatac
     3721 cttctccatc catcaaacaa aatgccgtct gaaacggaac aaaccctttt cagacggcat
     3781 cagatacctc caagctgccg gcaatcagtg gtggtgatga ccgtgcgggc cgtggacatg
     3841 accgtgtgcg atttcctcat cggatgcatc gcgcacgctt tcaactgtag ccttaaagcg
     3901 gattttcatg cctgccaaag gatggttgcc gtccaccacc gccttgccgt cggcaacatc
     3961 ggttacacga tagacgacaa catcgccggt ttcaggatcg tcggcttcaa acatcatgcc
     4021 gacttcgact tcaacaggga acacgcccgc atcttcgata cggaccaact ccggatcctg
     4081 ctcgccgaac gcatcgtcgg gcgacagcgc cacatcgacc gtatcgccgg catccttacc
     4141 gtgcaacgcc tcttccacca aagggaaaat gccgtcgtaa ccgccgtgca gatacgcaat
     4201 cggttcttcg gttttgtcca aaagctgatt gttggcatca tacatctcat aatgcagcga
     4261 aaccacggaa tttttcacga tagccatatt tgtcctttca ggaacagcag attaattaca
     4321 ggcgcattct aacacaaccg ccgcgccggc cgattaccgt taacctgttc ataaactgta
     4381 cagcacatat ttcaatgtaa atctttgtta ttttattgcg gtgtaacttt tttacaacat
     4441 tcttaaaacc attccgacct gtctgccgac tttcccaatc cgccttaata aatcatacaa
     4501 gatactgaaa ttatattaat ctctataata tttatcccta tcgaattttt aacagcaaaa
     4561 ccgttttaca ggatttatca atccgcccgc cagaaaactt ttcattcaaa cctttttccc
     4621 atctgtacga cattgcaatc ccttattcca tagtgcataa ttacgcaaat tcagcgatga
     4681 atttccaacc cggtttgtag tatggtcgat aaagacctat ttgtttcaat aatttaaatt
     4741 ggttctaaag gttactaaaa tgaaaaaatc cctgtttgcc gctgctttgt tgtctttggt
     4801 tctggcagcc tgcggcggtg aaaaagccgc tgaagctccc gctgctgaag cacctgccgc
     4861 cgaagctccc gctactgaag cacctgccgc cgaagctccc gctgctgaag cacctgccgc
     4921 cgaagctcct gctgctgaag ctgccgctac cgaagcacct gccgctgaag ctgccgctac
     4981 cgaagcacct gccgctgaag ctgccgctac cgaagcacct gccgctgaag ctcctgctgc
     5041 cgaagctgca aaataagcat tttccgcttg caaaaaagca ggatacgttc agtatcctgc
     5101 ttttttgatt tttcagacgg catcagattc ccttcctcaa tcttctccct acccttccga
     5161 caaacatgct tgaccttcat accgaatttt cccgactcct accggcagat gaaattgccg
     5221 aaccttctcc gacgctttta aaagaccagc gcaaccgctt tacgtctgca ccagacatca
     5281 ttttgcagcc gctcagcgtt aaaagcgtgc aaaccattat gcgtttctgc caccaacacc
     5341 gtattccggt tacgccgcaa ggcggcaata ctggtttgtg cggcgcggca gtatcggaaa
     5401 acggcgtatt gctgaacctt tccaaactca accgcatccg cagcatcaat ttgtcagaca
     5461 actgcataac cgtcgaagca ggttccgtac tccaaaccgt ccaacaggca gccgaagcct
     5521 caaacaggct gttcccactc agtctcgcca gcgaaggctc gtgccaaatc ggcggcaaca
     5581 tcgcctgcaa tgccggaggt ttgaacgtat tgcgttacgg cacgatgcgc gacctggtta
     5641 tcggtttgga agtcgtcctc cccaacggcg aactggtttc ccatctccat cccctgcata
     5701 aaaacaccac cggctacgac ctgcgccatc tgtttatcgg tagcgaaggt acattgggca
     5761 ttatcactgc cgccacgctc aagctgtttg ccaacccctt agacaaagca accgcatggg
     5821 tcggcatacc cgacatcgaa tccgccgtcc gcctgctgac cgaaacccaa gcacactttg
     5881 ccgaacgcct atgcagtttt gagctgatcg gccgttttgc cgccgaattg tcttccgaat
     5941 tcagcaaact ccccctgccg acacattcag aatggcatat tttacttgag ttgaccgact
     6001 cattacccga cagcaatctt gatgatcggc ttgtcgaatt tctttataaa aaaggcttta
     6061 ccgacagcgt gttggcgcaa agcgaacaag aacgtatcca tatgtgggcg ttgcgcgaaa
     6121 acatctccgc atcgcaacgc aaactgggca ccagcatcaa acacgatatt gccgttccta
     6181 tcgggcgcgt tgccgacttt gtccgccggt gcgccaaaga tttggaacag aatttcaaag
     6241 gcatacaaat cgtctgcttc ggacatctgg gcgacggcag cctgcactac aatactttcc
     6301 tgcccgaaat cctcagcaat gaagtctatc gttacgaaaa cgacatcaac agcacagtct
     6361 atcgcaacgt ccttgcctgc aacggcacga ttgccgccga acacggcata ggtatcatca
     6421 aaaaacagtg gctggacaaa gtacgcacgc ctgccgaaat cgccctgatg aaaagcatca
     6481 aacaacacct tgatccatat aacattatga atccgggcaa actgcttccg taaccggcat
     6541 ttctgatttg catacacaac aaagaaaggg acaatagatc cgattgtcgg tttagcgcga
     6601 gctcgtgagt gcggttaaaa attggtggaa attacacgaa aaatgaccgc acttttaaaa
     6661 taaaaaaatc ggcagtgaat ttccctgccg attttatttt gttacaactt aacttaaaac
     6721 gtccactgta aattcaacgc accttgttta gcttgatgat gtttgcctgt ttggcggttg
     6781 aatgtggctt gtaaggttaa gtgagatttg attttcactg ctacacctaa ttggctctca
     6841 attgccgtct tattgtttat cactcgacgc tctccgtcca tttccacacc gaaaggtttg
     6901 ttgtggtaaa gcgcgttcac agcggcgaaa ggttcaatag cgatattttt atagagtgaa
     6961 aattgagctt tagcttgaac gccaacccga gtttgtaatt ggcgggagcc aagtaaattc
     7021 acgtgggcat tttcgctatc gctgaatttt ccgtttaccc ccaaataagt caattgtgcc
     7081 tgtggttgta ggtaaacacg aaggctgttg ccctttttag tgaagtgttc cgccaataac
     7141 gcattgtaac ctgcttcaat tgaggcagta ataccttttg aagtaaaacg ttctgtacca
     7201 tcttcagtgt tgatacggtg gcggaagcgt tgatattgca tccagctatc cgcatacgca
     7261 cctgtctgtt tgtcctgaag ttggtgccaa gtggcgtaaa cgcctgcacc aaagcctttc
     7321 acatttcccg ttgtaagatt gtctgtatct gggttgtgga aagtgctacg ttgttctgct
     7381 tgtccgccca ttaagccaat agaaagttga ttactttcgt tttgccatgt gaatacttcg
     7441 ccgccgagtt gcacaccttt acgatagcct tctacaggtg ctgttttgcc ttgcacccat
     7501 tggttggaat gtccgtcaat cacacgcaac cacaagcctt tgcgtggtaa agtgcggtcg
     7561 aaaatatcgc tgtttttgtt gttcaaacgc aaggcgaata aggtattggc ggcttgagcc
     7621 tgttgtgcat aaatcgccat atcatcgcgt tcttgcactt tggtaaaaaa gccctctggg
     7681 cgttgttgta aagaaagcgt ataaattccc ttttggtgtt tgccagaaag acggaatgcg
     7741 tgtttatctg ctgtgccatt tactttgata atttgatgcc catcgaggct ttttaaatcg
     7801 tctattggat tttcgaagat gatgtcggaa gtgccagtaa catttttctc aaaaattaat
     7861 gcagtatttt tcgcttcttt aggatcgtaa gcaaaacgaa aacgagctcc gccagcataa
     7921 tcttctttta cgagtaaact ttcactttta gtattaaaac ggatgtctgc attcgttgtt
     7981 tttaatttcc caacattaga atcccaacgg ggctcccaga gagaattttc taagcggaat
     8041 tcatccaaac taatcgtttg cccgataacg tgcgagttgt ctgtaacctc aatatagtgg
     8101 aatggatcta aaccagaata tagatgtgct gcaaaagaaa cataattttc aatatgatga
     8161 attacttgat tagcccattc tgtataattc ccgacagata aaatttcgct gttgatatga
     8221 ctatttttta tttttggacc taaggagaat atatgacttt ttactataag aggatgggat
     8281 ccaaattttt cagcttggca agtactataa tcacgtatct tagtgttaga attaaaacat
     8341 tccttaaaat atttccgtat ttgttcttct gtgtccccat ttctttttgc aacccctaaa
     8401 cctcgggcga agccaactag gtaaccttcg gtatattctt gatcataaaa agaaatcttt
     8461 tttgagttat tgatgttttc gaattggtat gttctagggt atagtgcggg aaagggtgga
     8521 acttttggat tatcctcggt tataagataa gtttcttttt tccaatattc actcgtttta
     8581 tcgcggagtt tttttaagcg ggtaatttca tcattagtga gcttggtttt gtcgtaaacg
     8641 taatcaacag ccaaaagcgg agaggtataa agaatagaaa aaaatagact tacaataaat
     8701 gattttttaa acttctgctt gcttgcttgc ttcgagtttc ataataaatt ttcctttgtc
     8761 aagtaaaaat aaatggggcg tggattttag cataaaactg aacaaaaaat gtcatttatc
     8821 tcacattttt ctctatttat ttcttgttta ttaaaagtaa acgtttgctt tttgctattt
     8881 tgtcaagcca gtttgaaaat gtgtataatt gccctcgtta tttacaaaaa tttcaggaaa
     8941 aatgaccgca ctttaccctt ggctaatgcc aatttatcat caaattgctc aaacctttga
     9001 cgaaagcttg gggcatcatg ccgtgctgat taaagcggat gctggtttag gtgtagaacg
     9061 tttacacatc aggcggcagc cttgcccata ccgtctgaag cactgtttcc acaatcagcg
     9121 cgtatgctta atcaaccgct gtttctcgcg tttccaatcc gcctctttca tactctggcg
     9181 tttgtcgtgc tgtttcttac cttttgccaa accgatttcc atcttgattt ttccgcgtga
     9241 aaaatgcaaa tccagcggca cgatggtgta gccggcacgt tcggttttgc cgattaattt
     9301 gttgatttcc gactggttca acaagagctt gcgcggacgt acggcatttg gtttaatgtg
     9361 tgtcgaggct gtgggcaaag ccgtaatatg gcagccgacc agataaaacg cgtctttttt
     9421 ccaatagata taactctctt taagctgtac gcgcgcggcg cggattgctt tgacttccca
     9481 gccttccaag accaaaccgg cttcaatccg gtcttcaatg aaaaaatcgt gaaatgcttt
     9541 tttattgttc gcaatagcca taaacatcct atcaatatcc gccgtcagac ggcataaacc
     9601 cgaaaacaga acccatcata ccgcctcttc aaccgcctgc acaatcttct cgggatacag
     9661 cctgttgagg cagtcggtat gccccagcgg acattcccgc ttaaaacacg gcgaacattc
     9721 caagtgcagg ctgacgattt tcgccctatc gctcaaaggc ggcgtatgcg tcgggctgga
     9781 agaaccgtaa accgccacca ccttcctgcc caaagctgcc gccaaatgca tcaatccgct
     9841 gtcgttacac acgaccgtgt ccgccaacga cagcaaatcc attgcctgcg acaaatcggt
     9901 tttgccgcac aaattgacac acataccgtc tgaaaggcgg ttgatttcct cggcaatttc
     9961 atcatctttt tgcgaaccga acagccaaac ctgccaaccc gccgccagat aatgtttgcc
    10021 caactcggca aaatgccttg tcggccaacg ctttgccggc ccgaattccg cacccggaca
    10081 aaaagccaga acaggctttc caatatccaa gccaaaggtt tcgacagaaa tttcccgccg
    10141 ccgttcatca atggaaaact cggggaatcc cgaatgcccg tcaaaatctt cctgactcgg
    10201 atgcgcgaga gccgtatatc gatccaccat caaaggcaga cgttccttat ccagcctgcg
    10261 tatatcgttc aacagaaaat aacggctttc accgacataa cccgtccttt taccgatacc
    10321 tgtcgccagc gcgatgattg ccgatttcaa agaaccgggc aacacgataa cctgatcgta
    10381 tccgcgccgc cccaaatccc taccgacccg ccaacggcgt ttcaactcca acgcaccatg
    10441 tccgaacgaa ttctcaagaa tttcattcac ttccggcata cgctcgaaca ccgccatcga
    10501 ccacttcggt gcgaacacat caatcgtgca accggggtga agttccttca aacggcggaa
    10561 caagggctgg gtcatcacgc agtcgcctat ccaactgggg gaaataatca ggattttgat
    10621 ggacataaca agaaaccgaa atcagacagg cagaatttta ccgcgaaacc gttggaaaac
    10681 ctatcttgcc gcattccgaa cgccggacgt gcaaatatga aaaagcccga acattcaagt
    10741 tcgggcttca aaattctggc tccccgacct gggctcgaac cagggacctg cggattaaca
    10801 gtccgtcgct ctaccgactg agctatcggg gaatggggcg tattatagcg tccggaaaaa
    10861 atgtgtcaat ccttaatttt ggaaaaatgg gcgacaaaac gacaagcata tgaatcagaa
    10921 agacattaag accgatgcct taaaaggatt gccgttgtat gaatttccac agccgtcatc
    10981 acaccatatt taagcccgat gagccgttct gccctccccc cgcttaaaac aatgccgtct
    11041 gaacttcgcc gtgttccaaa gccagtaaaa actgtttgcg gttcaacccg cccgcgtagc
    11101 cggtcagttt gccgtcgctg ccgatgacgc ggtggcaggg aatcaggata gatactttgt
    11161 tctgcccgtt ggcggcggca acggcgcgga cggctttggg gttgcccaaa cgctgcgcct
    11221 gctccttgta gctgcgcgtt tcgccgtaag gaatcgccaa gagcgcgtcc catgcctgct
    11281 tttgaaactc ggtgccaatc tgctccaaag gcgtggcaaa ggttttcaga cgacccttga
    11341 agtataagtc caattcctgc cgcaaaagtt gcgtccgctc atcctcccga aacacaaacc
    11401 gtccgcgcaa ggctttttgg acggcggcaa tttcctgttc caaatgcttc tgtccgacaa
    11461 attccagcaa acacaaaccc ctgctaccga acaccgccag catctcgccc aaaggcgtgg
    11521 caatggcggc acacaccagc tcgttcaaac tgtcgggata acgcgcttcc aacagacgga
    11581 tggcgcggcg gatgcggaca tattcttcag gcgcgcagcc gatattgtcc caaaaatccc
    11641 gctcgaactg tttggcttcg cattccgtca gattgggatg cggcataacg ccgcactcaa
    11701 aaacccgaga ttcgagccaa tggcggattt catcccattt tgacggcaga ttgtttaagg
    11761 aaggcagggt aatcatttgt ttgctccgta tccctatcat agatttgacg gcaaaatccc
    11821 caatttttgc cattcccgca cgccggagca ggaacgggct atgacgtaaa tcttgagggt
    11881 taggttgcgg caatacctaa atattcgata tttctaaagc atcagagaaa ggaatgtttc
    11941 aacacacagg acgacacata aagcgccgcc ccatgaaaaa tttcagacga cctgcaaagg
    12001 gtcgtctgaa accacgattt ttgcatttgc gcattctggc acatcatcca accgtttcgg
    12061 cacattcctg ccgccgttga cagcctataa tgaatccact tattcatcaa gcaaaggaat
    12121 catctatgca aaccctcatc ctctccgccg tactgctggc tttttcaacc gctgcctttg
    12181 ccgggggcgc attcacgctg caattcgaca acccgtccga agacggcggc ttcacgcaaa
    12241 accagctttt gagcgcgcct tacggctttt gctgttcagg cgacaatgct tcgcccgcgc
    12301 tgtcgtggaa aaatccgccc gccgggacaa aagtttcgtc ctgaccgttt acgataaaga
    12361 cgcgccgacc ggactgggct ggatgcaccg ggtggtcgcc gacattcccg ccgatgtcca
    12421 ccgccgcaac gcgacctcgc tgcaattaag ccgctgcgcc aacatcgccg accggactgg
    12481 gctggatgca ctgggtggtc gccgacattc ccgccgatgt ccgccgccgc aacgcggcct
    12541 cgctgcaatt aagccgctgc gccaacatcg ccgacgacca gtccgcagcc atatcggcgg
    12601 taatcagttt gcggatttgc cgcatcaggt tgacgccttc gtacacggca aaaccgatgc
    12661 cgtcatgctg caaccacgcc aacacgccgc aaagcgcggc ctccgcagca ttgtgcggca
    12721 cttcttcatc cgccagtacc gcagcctcat aatcaaacgc cgcgcccata cgcccggaat
    12781 acggcagctt taccgcatcg cacactgcct gcgccgtccc gtattgtgcg gcgaaccttt
    12841 ctacggtttc ctgttcgaaa gcaatccatt gcgcctgata gaggccgtct gaatcgggaa
    12901 tattgatgac gtcaaacgtc tgtccgcctg ccaaggcgac cgccttaccc gccgcagctt
    12961 cttacttccg cgccgcacga taagcacagc cggttcatat accgccacgc tgcggtacaa
    13021 ggcggtatga tgttgcacga tgccgcctaa agcacccaat cgttcgcgcg tatgaaagta
    13081 tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac aaatcgtacg
    13141 gcaaggcaag gcaacgccgt actggtttaa atttaatcca ctatatctca aacccacgtt
    13201 aggtctaagc aaatggtcgg acatccttat ccgacagccc atcttctttt cagacggcat
    13261 tgcaaattta agtttgacgt gcgttcaaaa taaggcagtt aatgcgaagc gaaattccgt
    13321 cggcgtacct gcaacttggc ccctccccta taggggaggg tcggagggag ggtaaaacgg
    13381 ggcagataca gacaatattt ccgttgccgc cccgatgccc tctccctaac cctctcccac
    13441 gggagaggga atggattgcc gttgaaataa atcgctctac ataaaaaatc aatgtgttat
    13501 ctcaaaccca cattaggtct aatcaaatgg tcggatatcc atattcggca agcaagctgc
    13561 tttcagacgg catttccagc caacaagcgc gccaatatcc cctcatacac cgcagacagc
    13621 ttcggaatgt cgtttagccg cacgttttcg ttgatttggt ggatggtcgc attggacggg
    13681 cctaattcga taagttcttg cgcaatggct ttgatgaagc gtccgtccga agtgccgccg
    13741 gtggtggaca attcggcctc aatgccgcag gtttcggcaa tggctgcgcg tgccacgtcg
    13801 gtcagtttgc ccgcttgggt cagaaagggc tgccccgaac acgaccactg caaatcgtat
    13861 tgcacgccgt gtttgtccaa aatggcgtgg acgcgttgtt tcagccctgc ttcggtggac
    13921 tcggtggaga agcggaaatt gaatttgacg ttcagctcgc ccggaatgac gttggtcgcg
    13981 cctgtgccgc cgttgatatt ggaaatttga aagctggttg gcgggaaata ttcgttgcct
    14041 tcatcccaga cttcctgcgt cagctctaac aaggccgggg caaaagtatg cacgggattg
    14101 attgccaaat gcggataggc aatatggcct tgcttgcctt tgacggtcag gttgcccgac
    14161 agcgagccgc gccgaccgtt tttaatcata tcgcccaatt tgtccacggc ggtcggttcg
    14221 ccgacgatgc agtagtcgat aagctcgtcg cgcgctttca atacatcgac gactttggtc
    14281 gtgccgtcca acgcgtcgcc ctcttcgtcg gaagtaatca gaagcgcaat gctgccttgg
    14341 tggttgggat gtttggcaac gaagcgttcg caggcggtaa cgaaacaggc aatgctggtt
    14401 ttcatgtctg ccgcgccgcg cccgtataat cttccgtcgc gctcggccgg ttcgaacggg
    14461 ggcgaatccc atttttcgac aggacctgtc ggtacaacgt cggtatgccc tgcaaaacag
    14521 acgacgggag ctttcgtgcc gcgtcgcaac cagatgtttt tggtgtcgcc gaaatggagt
    14581 tcttcagccg caaaaccgat tttgtgcagg cgttcggcaa ggagtttttg gcaatccctg
    14641 tcgtcagggg taacggatgg tcgggaaatc agctctttgg caagctctag ggattgagtt
    14701 tcggtcatat ttgttcactt ttgaaattag accgtctgaa acgttctgaa tgtgattttc
    14761 agacggcatt taggttaggt tggcatacgg ggtgggtatt ttacccatca gtcttctgaa
    14821 tcatttgccg tggcaggctt cgtaaagcgg cagcaaatct tccaccgttt ccgctatcca
    14881 tttcgcgaca tcctgcctgc ccaaatcgtc gcgttcgatg tgtttgccga tgcagaaaaa
    14941 gtcttcgtcg ttttgcaact ttcggtcgga ctcgttttgt tgcgcgacgg tgcggtagtc
    15001 gtcgtattcg ctttccgcac cgtgccacat atcgaaagaa gcgtattttt cggtatcaaa
    15061 attatccaac cagcggttgt aatcaggcag cgcgatgggg gaaacatcgg ctttatagca
    15121 gtgccaatcc aagctgacgc tcagacggcg gcggttgagc agtatcgaca aaatcgctgc
    15181 ggaattttta tattgttcgt atttgaagta ggcaaagaaa tgggcgcgaa cctgccagcc
    15241 gttacaccag cgttcgatgt gcggcggcgc aaacggcgca cccaattcgg cggcaacctg
    15301 ctgaatcagc tgctgccata tctgccagtt ttctttatag tcagccttga tttgcggaat
    15361 gctttcaggc tggtattttt taagctggga aaattggaaa aacgggatat tgaacaaatc
    15421 gcaacttttc ggggtcagca taatatatcc ttgagacgat tgtttcagac ggcattattt
    15481 gcgccggcgc gccgccataa tttcgccgat ttcggtcagt ttttcttttg ggataaaggt
    15541 gttgcccata tcaaacagcg gctcttcaat cgccaaatga acatcatatc ccgccacaaa
    15601 acgtttgaac gcttcctcat cggggacata agcgttgtct gcttcgagtt tggcaaattc
    15661 ggcggaaaca gccgcccagt tgtcgtgcag cccgatatgt tggcgcaaaa gctcgtccac
    15721 gctttcttgg gcttgcggcg catattgcag cagcagcggg aagaagtttt cttcttcgtc
    15781 ttcatggtgc agcggcgcgg caacgttgaa atactgggcg atttggcgga tggtttgcaa
    15841 aacaatctga ttgcagccgt tttcggcgat atagtccgac agcatggcga cttgtccgca
    15901 aaaacggcgc actttgccgt ggcaggcata cagcatttca atcggttcgg caaaggtaac
    15961 gcttttggtt tcaaacggat tcatgttttc gttctcaacg ggcacttttc aagcagtcat
    16021 tttataataa aacagcctgc acaaagcagg ctgtccgtct tttgagactt taagcggatt
    16081 aatcgaccaa agtcactttg ccgttcatca aagcaccgtg acctgggaag gtacaagcga
    16141 atttatattc gccgtcggcc aatttagcag gatccagagt cagggaagct tcttcgccgc
    16201 cgccgatcag tttggtatgg gcaacaacgc gtgcatcatc aggtttgaca tagtcggtat
    16261 cggcagcacc tacgccgtct ttaaatacgc cgtccatgtc ttcagctttg gcaatcacga
    16321 gattgtgacc catgctggct ttgggttgcg taccggtatg tttcagagtg atggtgaact
    16381 ctttacatgc tttgctgact tggatgtctt tggtgttgaa ctgcatattg tcgttggatt
    16441 cgacagttgc cgcacagttg ccggcagcag gggcttcggc agcatctgca ggagcagctt
    16501 cggcggcagg cgcttcggaa gcgggtgctt cagcagcagg agttgcctcg gcagcaggcg
    16561 cggcaggttc ttgagagcag gcagccaaac cgataacggc ggcagaaatc agagccagat
    16621 acgctttcat aacaaatctc caatcgataa aataatattc ggttttacag aaatcaaagt
    16681 gcaaccgcca ttaacaaaac cttgaaaaag attccgccgc gttgcacaaa cagatgtttc
    16741 ggagcggcat tttgctacaa atttcatttg aaatcaaagc ctgtttgcaa gtttacaatc
    16801 gtttacccaa aaaagggcaa ttttaccccg aacctatttc tttagtatta gacctattat
    16861 cctttacttc ttaatattaa cggatgttta cacaaattcc cgtatacatt ttatgcgcca
    16921 tgccttctaa ccaagtttgc caatgcctcc gccaattcgg gatgccgttt ttccaacttt
    16981 gccgccgccg aaccgaaact ctccagcgca gccttactca aatgcagggt attggttttc
    17041 ggcggttttt ccggtttcgg gaccagcctg accgaaacag agcgtatcga agcatcaagc
    17101 cctgccaact gcggcaatac cgacggtgca atcattttca agcgcgatgc cgccatattg
    17161 tttgccgcca aaaggacaag cctgccgtct tcgatacatg ccgtctgaaa atgcgggtgc
    17221 aggttggcag gcagcagttt tttcacggcg gcatccaacc gccgccactg tcccgcctgt
    17281 ttcaaaagtc cggaaagcag cgcgtcccgc ctgcccaact gttccaaatt cataaaacat
    17341 acacccaaaa agattgaaat accgcaaacg cgcctttatt tcagacggca ttagcacttt
    17401 gcacaaacgc ttgtgttaaa atcgcgtttt cgcccactat tatatcaggc gcaggaatta
    17461 ttcatgctga caaacattgc caagaaaatc ttcggcagcc gcaacgaccg cttgctgaaa
    17521 caataccgta aatccgttgc cagaatcaac gcgctcgaag aacagatgca agccctaagc
    17581 gatgctgatc tgcaagccaa aactgccgaa ttcaaacaac gcctcgccga cggtcagact
    17641 ttggacggca ttttgcccga agccttcgcc gtctgccgcg aagcgtcccg ccgcaccctc
    17701 ggtatgcgcc acttcgacgt gcagcttatc ggcggtatgg tgctgcacga cggcaaaatc
    17761 gccgaaatgc gtaccggcga aggcaaaacc ttggtcgcca ccctcgccgt ctatctcaac
    17821 gcgctggccg gcaaaggcgt acacgtcgtt accgtcaacg actacctcgc ctcacgcgat
    17881 gcgggcatta tggagccgct ctacaatttc ctcggcctta ccgtgggcgt gattatttca
    17941 gatatgcagc cgttcgaccg tcaaaacgcc tatgccgccg atatcaccta cggcaccaat
    18001 aatgaattcg gcttcgacta cctgcgcgac aatatggtta ccgaccaata cgacaaagtg
    18061 cagcgcgaat tgaattttgc cgttgtcgat gaagtggatt ccatcttgat tgacgaagcg
    18121 cgcactccgc tgattatctc cggtcaggcg gatgacaaca tccagttgta ccaaatcatg
    18181 aacaccgttc cgccccacct cgtccgtcaa gagacagaag aaggcgaagg cgactattgg
    18241 gtcgacgaaa aggcacatca ggtcatcctg agcgaagcag gtcacgaaca cgccgagcaa
    18301 atcctgaccc aaatgggatt gctggcagaa aacgactccc tctattccgc cgccaatatc
    18361 gccctgatgc accaccttat ggcggcattg cgcgcgcatt ccctcttcca caaagaccaa
    18421 cattacgtca tccaagacgg cgaaatcgtc atcgtggacg aattcaccgg ccggctgatg
    18481 tccggccgcc gctggtcgga gggtctgcat caagccgtcg aagccaaaga aggcgtggaa
    18541 atcaaacgcg aaaaccaaac gcttgcatct attaccttcc aaaactattt ccgcctgtac
    18601 accaagctct ccggcatgac cggcacagcc gataccgaag ccttcgagtt ccaaagcatc
    18661 tacaacctcg aaaccgtcat cattccgacc aaccgccccg tacagcgcaa agacttcaac
    18721 gaccagattt tccgttccgc cgaagaaaaa ttcgaagccg tcgttaaaga cattgaggaa
    18781 tgccacaaac gcgggcagcc cgtcctcgtc ggcaccacca gcattgaaaa ctccgaactg
    18841 gtatccaagc tgctgaccca agccggactg ccgcacaacg tcctcaacgc caaagaacac
    18901 gaacgcgaag ccctgattgt cgcccaagcc ggcaaagtcg gcgcgattac cgttgccacc
    18961 aatatggcgg gacgcggtac ggacatcgtt ttaggcggca acctgaagca ccaaaccgat
    19021 gccatccgcg ccgacgaaac cttgagcgac gaagagaaac aggcacaaat cgccgcactc
    19081 gaagacggct ggcaggcgga acacgacaaa gtgatggaag caggcggttt gcacatcatc
    19141 ggtacggaac gccacgaaag ccgccgcatc gacaaccaat tgcgcggacg ttccggccgt
    19201 cagggcgacc ccggatccag ccgcttctat ctctcctttg aagacccatt gctgcgctta
    19261 ttcgcactcg accgcgccgc cgccatcctc aaccgcctcg cccccgaacg cggcgtcgcc
    19321 atcgaacaca acctgctgac gcgccaaatc gaaggggcgc aacgcaaagt cgaaggcaga
    19381 aacttcgata tgcgcaaaca ggttttggaa tacgacgacg ttgccaacga acagcgcaaa
    19441 gtcatttaca gccagcgcaa cgaaattctg accagcaaag acatcagcga cctgatgcag
    19501 gaaatccgtt ctgatgtcgt cagcgacctc gtggatacct atatgccgcc cgacagcatg
    19561 gaagaacaat gggacatccc gactttggag aaccgtctgg ctgccgaatt cagactgcac
    19621 gaagacatcc aatcctggct gaaggcggac aatgcgattg acggtcaaga catcaaagaa
    19681 cgcctgatcg aacgcatcga aaacgaatat gccgccaaaa ccgaactggt cggcaagcag
    19741 gcaatggccg atttcgagcg caacgtgatg ttgcaggtca tcgacaacca atggcgcgaa
    19801 cacctcgccg ctatggacta cctgcgacaa ggcatacacc tgcgcagcta tgcccaaaaa
    19861 aatccgaagc aggaatacaa acgtgaagcc tttaccatgt tccaagacct gtggaacggc
    19921 atcaaattcc atattgcctc cctgcttacc tcggttcaaa tcgaacaaaa ccctgtcgcg
    19981 gtggttgaag agcaacccat cggcaacatc cagtccatcc attccgaatc gcccgatatg
    20041 gaagaacttt tgggtcagtc gcaaaccgat ctggttaccg aagcctttaa tcccgatggg
    20101 acagatttca gccccgaagc cttggaagcg cgggggcaaa tcgtccaccg caacgacccc
    20161 tgcccctgcg gcagcggttt gaaatacaaa caatgccacg gcaaactggc ttaagcgttt
    20221 gaacgcaaat gccgtctgaa catcccgctc ccgtttcaga cggcattttg cctgaaccgc
    20281 cacatccgac tgccattccg aaaaatcccg atttcgtacc gtccgtacca aaaacagaca
    20341 tcccgtccgc cccacatcat gattccatcc gacttcattg acgagctttt agccaaaacc
    20401 gatattgtcg atattatcga cgagcaggtt ccgctgaaaa aaggcggggc gaactatatg
    20461 gcgtgttgcc cgttccacaa ggaaaaaacg ccgtcgtttt cggtcagtcc aaccaagcag
    20521 ttttaccatt gtttcagttg cggggcacac ggctcagcga ttggttttgt gatggaacat
    20581 cagggactgt cgtttccgga ggcggttcag ttccttgccg accgcgtggg tatggtcgtg
    20641 ccgaaagtgc acgggcaaaa cgataatccc gaagtccgtg ccgaacgtaa gaaaaaacag
    20701 cagacactgg aggaaacgac ggctgcggca gctgattttt acgcgcaaca gctaaaattc
    20761 aatccagcgg caaaagctta tttggacaag cgcggcttga gtgcagaagt tatcgcgcat
    20821 tatggtttgg gctatgcgcc cgacggctgg cagcctttga cgcaagtgtt ccaaccgtat
    20881 cctaataccg cgttagtgga tacggggatg gtgattgaca atgagggacg gcattacgac
    20941 cgcttccgcc atcggattat gttccccatc cgcaatccgc gcgggcaggt tatcggtttc
    21001 ggcggcaggg tgctggacga ctcgaagccg aaatatttaa attctcccga tacgcctttg
    21061 ttcgataagg ggaaaaacct ttacggactg tatgaagggc gtgccgctgt caaggaagcg
    21121 gggcggattt tggtggtcga aggctatatg gacgtggtcg cgctggcaca gttcggcgtg
    21181 ggctacggcg tggcggcttt gggtacggcg acgacggcgg aacacgtcaa aatcctgatg
    21241 cgtcaggcag acagtattta tttctgtttc gacggcgaca gcgcggggcg aaaagcggct
    21301 tggcgcgcgc tggaaaacgc gctgccgcag ttgaaggacg acaaatcgct gcattttttg
    21361 ttcctgccgg aagaacacga ccccgacagc tacatccgcg cctacggcaa agcgcaattt
    21421 gaagacgcgc ttctgaatca aagcaagcct ttgtcggagt atttctggga acacctttca
    21481 gacggcattc atctcaatac gcaggaaggc aaggcggaat tggtaaaaac cagttcgccg
    21541 cttttggcgc agattaccgc gccggcattg gcttatttgt taaaacaacg gcttagcgag
    21601 ctggtcggca tcgaccccga caacctcgcg caactgctag gacaggaagc gccgaagcgg
    21661 cacgtcaaac aaaaaaacta caaactgcct ccgatttccg tcaaacagcc cgtcatgctg
    21721 acgctggtac agcggcaaat ccgcagcctc ttgataaatc cggattgggc tgcatatata
    21781 gacctgcccg attatctggc gttggacggt gatttcgcct gccttgccaa tcttgccgaa
    21841 tcgattaaaa accatgccgc cgtacccgaa accgctcagg ttttagagta tatgcgcggc
    21901 tcgccttacg aagaaacgat aacccgaatc ttccattcaa cgcaccaatc ggaagaaatg
    21961 aacagcagca gtgaagaaga ttgcgagaat ttccaaatcg gcatgaaaaa actgctcaat
    22021 gagttaaaat acagccaaat cgaaacatta aaacaaaaaa gcctgcaatc cggcttaaat
    22081 gaaagcgaga aaaaactttt gctgtcgctg ctgaccgcaa aacaaaattg accggcggat
    22141 tccgccatcc gtaaaccgtt atgccgtctg aaaagcattc accccggctg caacaacgac
    22201 acctgcagaa cacccatccc caaaagcctt cagacggcat cagagtaccc tactctgcca
    22261 cgccttcagg tgcgtccaaa cgcaaaccgt cggcatctta ccaacagaaa gcagacaatg
    22321 tccagaaacc aaaatcacga agaatatcaa gacgacaccc gtccgttaag cattgaagag
    22381 caacgcgcgc gcctgcgtca gctcatcatc atgggtaaag aacgcggcta catcacctac
    22441 tccgaaatca acgacgccct gccagacgat atgtctgatg ccgaccaaat agacaatatc
    22501 gtcagcatga tttccggttt gggcatccaa gttaccgaac acgcccccga tgcggaagac
    22561 atattgttaa gcgacaatgc cgccgttacc gacgatgatg ccgtcgaaga agccgaggcc
    22621 gccctttcca gtgcagattc cgagttcggc agaaccaccg accccgtccg tatgtatatg
    22681 cgcgaaatgg gacaggtcga cctgctgacc cgcgaagacg aaatcatcat cgcaaaaaaa
    22741 attgaaaacg ccctgaaaaa tatggttcag gccatctccg cctgcccggg atccattgct
    22801 gaaatcttag aactcatcga aaaaatccgc aaagacgaaa tccgcgtcga cgaagtcgta
    22861 gaagccatta tcgacccgaa tgaagtattg ctcaacgaat tgggcttggg gcacttggaa
    22921 accacagcgc ccgagaaacc ttccaacgac aattcggacg aaaacgaaga cgacgaagaa
    22981 tcggaagaag atgcggatga aatctcggca gccaatctcg ccgaattgaa acaaaaagtc
    23041 atcggccact ttgcccaaat cgaaaaagac tacaaaaaaa tgattggccg tttggaaaaa
    23101 caccacagcc ggcacaaaga ctatctcgcc taccgcgacg cgattgccaa caaactgctg
    23161 gaagtccgtt tcgccacccg gcaaatcgac agcctcagca gcagcctgcg cgggaaagta
    23221 gaaaacatcc gcaaactcga acgcgaaatc cgcgacatct gcctcgaccg cgtccatatg
    23281 gaacgcgact acttcatcca aaacttcctg cccgaaatca ccaatctaga atggattgaa
    23341 gaagaaatcg ccaaaggcag ggtttggagc gacgcgctcg accgcttccg ccacgccatc
    23401 ctcgaaaaac aaaccgagtt ggcggatatg gaaaaagaaa cccgcatttc catcgaagag
    23461 ttgaaagaaa tcaacaaaaa tatggtgtcg agcgaaaaag aaaccgcagc cgccaaacag
    23521 gaaatgattc aggcaaactt gcgcctcgtg atttccatcg ccaaaaaata taccaaccgg
    23581 ggcttacaat tccttgatct gattcaggaa ggcaacatcg gtttgatgaa agcggtcgat
    23641 aagttcgaat accgcagagg ctataaattc tccacctacg caacctggtg gatccgccag
    23701 gcaattacac gctcgattgc cgatcaggcg cgtaccatcc gcattccggt acatatgatt
    23761 gaaaccatca acaagatgaa ccgcatctcg cgccaacacc ttcaagaaac cggcgaagaa
    23821 cccgattccg ccaaacttgc cgaactgatg cagatgcccg aagacaaaat ccgcaaaatc
    23881 atgaaaatcg ccaaagagcc gatttcgatg gaaaccccca tcggcgacga cgacgattcg
    23941 cacttgggcg acttcatcga agatgccaac aatgttgcgc cggccgatgc ggcaatgtac
    24001 accagcctgc acgaagtaac caaagaaatc ctcgaaagcc tgacaccgcg tgaggcaaaa
    24061 gtcctgcgta tgcgtttcgg catcgatatg aacaccgacc acacgctgga agaagtcggc
    24121 agacagtttg acgtaacgcg cgaacgcatc cgacaaatcg aggcaaaagc actccgcaag
    24181 ctgcggcatc cgacaagaag cgaccgtttg agaagtttct tggacagcga agacagcaag
    24241 ctgtaaacca aaaaaccgca ggtttcaaat acctgcggtt ttttcttaca caataaacaa
    24301 cgcttccaca tatcccacac tcctatcc
//
LOCUS       DAWISC010000011        22683 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWISC010000011 DAWISC010000000
VERSION     DAWISC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.011"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(120..1034)
                     /locus_tag="WIW87_000312"
     CDS             complement(120..1034)
                     /locus_tag="WIW87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5173529.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIW87_000313"
     CDS             1290..2759
                     /locus_tag="WIW87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ5173530.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WIW87_000314"
     CDS             3011..3382
                     /locus_tag="WIW87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173531.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WIW87_000315"
     CDS             3455..4864
                     /locus_tag="WIW87_000315"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ5173532.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WIW87_000316"
     CDS             4910..5704
                     /locus_tag="WIW87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173533.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WIW87_000317"
     CDS             5892..6668
                     /locus_tag="WIW87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ5173534.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="WIW87_000318"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="WIW87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6921..7064)
                     /locus_tag="WIW87_000319"
     CDS             complement(6921..7064)
                     /locus_tag="WIW87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173535.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
     gene            complement(7740..10211)
                     /locus_tag="WIW87_000320"
     CDS             complement(7740..10211)
                     /locus_tag="WIW87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ5173536.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(10208..11386)
                     /gene="hflX"
                     /locus_tag="WIW87_000321"
     CDS             complement(10208..11386)
                     /gene="hflX"
                     /locus_tag="WIW87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ5173537.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(11391..12671)
                     /locus_tag="WIW87_000322"
     CDS             complement(11391..12671)
                     /locus_tag="WIW87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ5173538.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12824..13549)
                     /locus_tag="WIW87_000323"
     CDS             complement(12824..13549)
                     /locus_tag="WIW87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ5173539.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13622..14299)
                     /gene="radC"
                     /locus_tag="WIW87_000324"
     CDS             complement(13622..14299)
                     /gene="radC"
                     /locus_tag="WIW87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ5173540.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14422..15771)
                     /gene="gshA"
                     /locus_tag="WIW87_000325"
     CDS             complement(14422..15771)
                     /gene="gshA"
                     /locus_tag="WIW87_000325"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ5173541.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            16031..17440
                     /gene="leuC"
                     /locus_tag="WIW87_000326"
     CDS             16031..17440
                     /gene="leuC"
                     /locus_tag="WIW87_000326"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ5173542.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17537..17791
                     /locus_tag="WIW87_000327"
     CDS             17537..17791
                     /locus_tag="WIW87_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173543.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17853..18494
                     /gene="leuD"
                     /locus_tag="WIW87_000328"
     CDS             17853..18494
                     /gene="leuD"
                     /locus_tag="WIW87_000328"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ5173544.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18669..18929
                     /gene="dcm"
                     /locus_tag="WIW87_000329"
     CDS             18669..18929
                     /gene="dcm"
                     /locus_tag="WIW87_000329"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5173545.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            18975..19490
                     /gene="dcm"
                     /locus_tag="WIW87_000330"
     CDS             18975..19490
                     /gene="dcm"
                     /locus_tag="WIW87_000330"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5173546.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            19505..19939
                     /locus_tag="WIW87_000331"
     CDS             19505..19939
                     /locus_tag="WIW87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ5173547.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19955..20116
                     /locus_tag="WIW87_000332"
     CDS             19955..20116
                     /locus_tag="WIW87_000332"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ5173548.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            20117..20686
                     /locus_tag="WIW87_000333"
     CDS             20117..20686
                     /locus_tag="WIW87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ5173549.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20720..21790
                     /gene="leuB"
                     /locus_tag="WIW87_000334"
     CDS             20720..21790
                     /gene="leuB"
                     /locus_tag="WIW87_000334"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ5173550.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDASYEYAPLGG
                     EAYDEYGHPYPQFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21953..22516
                     /locus_tag="WIW87_000335"
     CDS             21953..22516
                     /locus_tag="WIW87_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ5173551.1"
                     /translation="MKKIIIAALAAAAIGTTSAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPNIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     QWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5540 a         5914 c         5815 g         5414 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaatgtt tgcgcgggac acattgccaa
     4261 acaaatgggc ctgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgtaacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctgcggaaa tcaatacgct gtgggcggaa gtggctgcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggttttacct ctggcaaatc
     4561 cacccacgcc gaccgcctcg ccaccatcaa gcaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaat
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcacga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tgggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg atccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attctaccgc gctaatattg aaaaacggcg gactgctgga
     5521 agctgctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttgacttta tcccgaattg
     5821 ggcagatatt ctcattctga cacacgtccc aaataaacac tccccatttc ttttaccgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggccaat
     6001 tcgttatggt cgggctgatg gtggacggca agccactgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccttggt tgccggcgac ctgaatccgg gcaaacacct ttacctgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagatcccga aatttacgaa cagtttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggccg cctgaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaattaa tcctcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgc gcatttgtgg
     6661 accaataatc tgcttgaaaa tgccgtctga acggcatctg cgataaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaattaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaagtggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatgggtt
     6961 cccacttgcg ggggaatctt catcgtctca tttttgtttt catcaacttt tcctaaactt
     7021 tcctgtttgg ctgcataatc agacttgact ttgttatttt ccatattgag cttctcttaa
     7081 tttgaaaaaa ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaaataa
     7141 tgagaatata aaggataaaa agagagttaa gttatttaat cggacttgtt ctctataaat
     7201 cataagacac caaataagcc ataataaatt tagtaaataa aaactgctta tacccaactt
     7261 tcttttcttg gaacctatta ttatttcaac tccggatgaa aaaatagaat ttaaaaattt
     7321 ttttctttga tatatcgtta atatctccct agtttttctt aaaaaaatat aatcatatat
     7381 atactacaca acattggtaa taaataaatt aaaagcgaaa ggtaattaaa ttgataattg
     7441 taaattatat aataaacaac atacgaagaa aaaattatta tagatgattt actatacgaa
     7501 taaaaaaata tcctacctag cccgaacctt cttataattt gtactatgtg attatccatt
     7561 tcaatatcca tttaagcttt caattaattt tctatatttt tcaatggcat gtatcatgct
     7621 atgcttggga aatatcatct tggttgatga tcaagccatg tatgcaaaga ttggaatatg
     7681 gtatttgtcg tgctaataat gcccccgtta ctgttatacg tctaaagcaa cgctgtccgt
     7741 cattgttcgg acggcatttt ttcagacggc atgtccacct gcttgtgaag ccgcatagca
     7801 ttgcccaata cgaccagcga actgaagctc atacccagtg cggcaatcca cggttggaca
     7861 tagcccaaaa cggcaagcgg tacggcaatg atattgtacg cgcccgccca tatcaggttt
     7921 tgccggataa tatggcgggt gcgccgcgcc tgatcgagca ggtgggcgac ggtacgcaaa
     7981 tcttcgttca ataacacaat gtccgcgccg tccctcgcaa tatccgtccc gcccgctgcg
     8041 gcggcggata cgtctgcctg cgccaaaacg ggcgcgtcgt tgatgccgtc gcctatcatc
     8101 agcacttttt tcccttcttt ttgcaaggct ttgacgtatt ccagtttgtc ctcgggcatg
     8161 gcttgggcgc ggtagtgcgc gacacccagg gcgcgtgcgg tttcggcaac ggcggtttcg
     8221 cggtcgccgc tgaggatgtg cagggtcagg tttttgcctg ccaactgccg caccgcctcc
     8281 gccgcgctgt ctttcaaggg gtcggtcagg tagaacacgg cttggaaacc gctttgactg
     8341 ccgaggtaaa ccgcgctgcc gccgccttct gtttgcggtt ctttacctga aatttcggcg
     8401 acataggatg ccctgcccaa tgcccaaacc tgtgtttcgc cgttgacggt cagttgcgcg
     8461 cccacgcctt cgccgatgcg gttgaggcgt tgtttaatag cgatgtcggg gacgctgccg
     8521 cttgaaacac ggcagttgag gatggcgcgg gcaaggggat gttcggactg ttgttctaaa
     8581 gcctgcgcca ccgcgagaac aaaggcttcg tctgtgcctc tcaacaatga gatacggcgg
     8641 acggcgggtt tgccttgggt cagcgtgccg gttttgtcga agatgatgtc ggtggtttgg
     8701 gcgagggttt cgattgcctg ctttccgccg attaaaatgc cttcgcgcgc cagcgtaccg
     8761 gtagaagctg ccagcgcggt cggcgtggca agcgataagg cgcaggggca ggtaatgacc
     8821 agcagggcga cggtaatcca caatgcggtg tgcgcgtcgg cgtacagcgt ccagccgatg
     8881 aagacgggga cggcaagcag gagttcgccg aatatgaaag acgaggcgta ttgttccgcc
     8941 aactcggcag tgcgcggttt ttgcgctaag gcgcggtcga gcaggcggac gatgtgcgac
     9001 agtcgcgtgc cgccgccggt gcggtcggtg cgtataatca gggggctttg cgtgttgagt
     9061 gtaccggcgg ttactttttc agacggcatt ttggcgacgg gcaggctctc gccggtcagc
     9121 atagattcgt tgacggcact gcttccttcc agcaccgtgc cgtcaacggg gatggtttcg
     9181 cccggtttga ccagcacgat atcgcccgcc ttcaatttga cgacagctgc ctcgcaggtt
     9241 tcctgcgtat cggggtaatc gggcatatga tggcaaaacg caggaatcag cttcaccagc
     9301 ctctcggcgg catcgcctgc cttacggcgg gcaatgtgtt ccataaagcg tccgcccagc
     9361 aggaaaaaca gcagcatcgc gatggattcg aaatacatcc cctgccccgc atttgtcgca
     9421 aggctgtaaa cgccggcgat aaaggtcatg atgatggcga cggtaatcgg cgtatccatg
     9481 ccgacgcggc ggtttttcaa gtcgcgcagc gcgccttgat aaaacgggac ggcgcaatag
     9541 aatacgacgg gcagcaccat taaaaagccg ccccaatgga ggatttgcag gaaatcgggt
     9601 tcgatgtcgc cgccgtaaag gtaggtcggc agcgcgaaca tcatcgtctg catcatcccc
     9661 agcccggcaa cggcgaggcg gacgatgtat tgtttgcgtt ctttttggtt ggcggcttcg
     9721 attttttgcg cgtcataggg tgcggcggtg tagcctatct gcctgatttt caacagaatg
     9781 tcggaaaggc ggattttgcc gtcgtcccag acgacgcggc agcggtgcgt gctgtaattg
     9841 aggtcgatgc ggacgatgcc gtctgtacgc aaaagctgct gttcgatcag ccagacgcag
     9901 gcggcgcagg tgatgccgcc gagcattaaa accgcctcgc gcgtgccgcc gtgggtttcc
     9961 acaaagtcgg actggacttc gggcaggtcg tacaggcgga tttggtcgag gatttcttgg
    10021 ggcggcagct cggttttttg cgcgtcggcg gtgcgttgtt tgtaataact gcccaagccc
    10081 gcgtcaataa tgctttgtgc gactgcctga caaccggcgc agcaggtttc gcggtcttcg
    10141 ttttcgtaac ggacggtcag atgcaggttt tcgggaacgt ccagcccgca gtggaaacag
    10201 gtttttttca tggcatttcg gtttcgtctg tgtttggtgc ggcggcacaa tactcggcaa
    10261 tggcttcgcg cagtgcgtct ataccggtat tttcagcaac ggaaatgcgg acggcggcaa
    10321 tttttcccgc agcgtcgcgc catatgcccg tgttttgttc ttcagacggc agcaggtcgg
    10381 ttttgttgta caccttgatg cacggaatat cgccggcatg gatttcttgc agtacgtttt
    10441 ccacgtcttc aatctgctgt ccgctgttcg gagcggcggc atcgacgacg tgcagcagca
    10501 catcggcttg cgcggtttct tccagcgtgg cggaaaaggc ggaaatcagt ttgtgcggca
    10561 gatcgctgac gaatccgacg gtatcggtca ggataatgct gcattcggga ctgatgtaca
    10621 gccgccgcgc cgtcgtgtcg agtgtggcga aaagctggtc tttcgcatat atgcccgact
    10681 tggtcagccg gttgaacaga ctggatttgc cgacattggt atagccgacc agcgcaaacg
    10741 ttttgattgt gcccgattcg cgggacttgc ggcgcagggc gcgctgtttt ttgaggttgg
    10801 caagctgttt tttcaaggca ttgatccgat gggcgatcaa tcggcggtcg gtttccagtt
    10861 tggtttcgcc ggggcctttc atgccgatac cgccgcgctg gctctgcaga tggccgtaac
    10921 cgcgtatcaa gcgtcccgcc aaatggctca attgtgccaa ctcgacttgc agcctgcctt
    10981 cctgcgtgcg ggcgcggcgg gcgaaaatcg ccagaatcag ccctaccctg tccaatacgc
    11041 ggcatttcag ttctttttca aggttgcgtt cctgcgtggg cgtgagttcg tggttgaata
    11101 cgaccaaatc gatgccgtct gcggcaactg cttctgacag ctccgccgcc ttgcccgtgc
    11161 cgacaaacag cgcggtgtgc ggacggtcgc gtttggcagt ctccacgcgc acggaatcgc
    11221 cgcccgccgc tttgaccagc tcgacagctt ccgccaatgc cgtctgaaaa ccgttcagac
    11281 gggtggcact gctgcccgtg ccatctttgt ccaacattac gcccaccagc atgacgcgtt
    11341 cgggttgcgc ttgggtactg ccgttgcggc ctgttctgcc tgtcatgtat tcaatcctgt
    11401 aaataaaggg ttacgcctcg ttccgtttct ccttccggtg caagcgtgat gcggctgccc
    11461 gccattccgt caacgctgaa gacgatgccg cccgcctcgt gccttcctat ggacaggcgg
    11521 ctgccgggtc gctgccggta ggtataacgg gtacacgctt ccaaacacca gcgtccgcgc
    11581 cagtggatga aatatttttc gccttttcta accgcaagtg tctcgccgcc gtttacctca
    11641 aaataagggt aatcgggttt tcgggttcta aactgccatt tcgcctgcgc tcgccgtccg
    11701 ttgcggacat agtcgaatac cgccgtataa agcgtgccgt attccaaagg cttgagcgga
    11761 aaaagcgcga attggtacgc ggtcagcctg ccgttggggt cgttgccggc ggttaaaacc
    11821 ctgacggggc ggatttcgtt tttaccctga tacagcttga aacttttcat cgtaattttg
    11881 cctgccgcct cggaaaaatc aatgctggca ggattgcccg tgatttcata ttccggcacg
    11941 gggtctggac gctcgccgtg gaaataaggc agtgcgccgt tgccgacggg ataggctgta
    12001 tagagcagct cctgtgcggg catggcttcg tcggtgtaca cgaccgcacc gttatggcag
    12061 gcgttgcggt aatatttccg tcctgcttcc ggctgatttc tgccttgggc gcaatgccgc
    12121 tcaaacctgc cgttgccctg attgaatacg agaacggttt taccgttttc gcgcacaaat
    12181 gccgctcctg cctcatccgt atggcggtca agtagggaaa ggcggtggta gattgcgctc
    12241 attaatccgt ccacttggcg ttgctgcgtg cggatgtcgc tgtcggacga ttcggcggct
    12301 tcctcttccg tgctgatgtt ttcatgcacg ccgttgtaga gatacccggc aaggcgtgtg
    12361 cgttcggtca gcttttgtgc ggtgtagtgc ggattgtcgg gatggtgttc gccgtgtccg
    12421 tcttcgggat tgagcgtgag atagcgtgcg tgcctgcggg cggaattttc caaaaccggc
    12481 gcgtgtgcca gcttgtgcaa accgatttgg gcgcggatgc ggttgaggta gtgcagcgca
    12541 tcgccgccgt ccctgatttg cggtgcggac ggatagacaa gttcgcccgc gggcagggat
    12601 tggttttggg tatgatagaa aacgccggcc gccgaaccca acaggagcag ccgaataaaa
    12661 agggatttca taatgccgtc ctcccgacgc ggcaacgggg tgttttatca aaacggcaaa
    12721 cttgccaatg cggtttccga ttgcagattg ccaaaccggc aaacctttcc ccgtgccgat
    12781 gccgaacccg atgccgtctg aaccttcaga cggcatcggg tgtttatttg tccacccgga
    12841 tgggtgcagg ttcgtattgt gtcgattcgt cgccgtaata cagcacgccg agtttgatgg
    12901 ggatgcgtcc ctgcgatttg cggtgggcgt tggaatccct cagggaatag gcacaaccgc
    12961 aatattcctg ctggtagaag ttttcacgtt tgctgatttc aatcatacgc gcgctgccgc
    13021 cgcctttgcg ccagttgaaa tcccaataca ccacatcatc gtaaggcgcg gcggcgcggt
    13081 gtccgcagtc gttgatttgc gccatatttt tccagcgtga aatgcccagc gaactggtaa
    13141 agacggggaa cccgtgttca tgcgcgtatt gcgccgcctt ttcaaaacgc atatcgaaac
    13201 acatggtgca gcggatgccg cgttccggct caaactccat gcctttggct ttggcaaacc
    13261 attctttgcg gtcgttttcg tagtcgtcgt ctttatcgat gaaaggaatg ccgaactttt
    13321 ccgcaaagcg tacgttttcc tcttttcgga gcatatattc tttgtgcgga tggatattgg
    13381 gattgtaaaa ataaatggtg tagtcgatgc cggaggcaag catggcttcc atcacttcgc
    13441 cgctgcacgg ggcgcagcag gaatgcagca agactttttt atgtccgccg ggcgggacga
    13501 gtatggggcg gtcaatgtcg gtaacggtag gtttgttttg tgtttccatt aacttaacaa
    13561 tatctgtcga atacggttga agcggctatt ttatcagagc cgccgcatgt aaaacagggt
    13621 gtcagggcat cagcccgagc tgcctgaacg aacggacggt ttgcgccgtt acgataaaat
    13681 ggtcgagcag cgacacatcg accagcgaca tggcctgtgc cagccgcctt gtgaacatga
    13741 tgtcttcctg cgaaggttcg ggcgagccgc ccggatggtt gtgcgcaata atcaggctgt
    13801 cggcatattc gtccaatgcc agtttgacga tttcgcggat gtaaatcgtg ttttccgcaa
    13861 ccgtaccgcg cgacagctct ctgaccgcaa tcagttggtt ttggcggttc agcagcagcg
    13921 cgacgctgac ttcgactttt tcctgcccca aatgaaagcg taaataatcg gcaacggtat
    13981 ccggatcgga caggacgatg ctctcctgca attcttccgc caatatccgc cgcccgattt
    14041 ccctgactac ggcaaactgt gtgaaacttg ccgtccccat ccctttgtat gccgacagtt
    14101 tgccgacctc cgcgctcatc agcctcccca aactgccgaa ctcctgcagc aaataacgcg
    14161 ccaaatcgac cgcactcatt ccgcgcgtgc cgacacgcag caggattgcc aaaagttcgg
    14221 catcactcaa agccgccgcc ccgcgttcca acagcttttc cctaggtctt tcgccttccg
    14281 gccattgctt gatgctcatg ttttcccttt tttaaatagt ctgagatgcc gtctgaaaca
    14341 aatgttccca cacgccaaat atccgaaatg ccgtctgaac cggatttccg cttcagacgg
    14401 catctgtatt tcaaaacagt cttacgccgt ttcttccaac tcgatggacg ccgccagaag
    14461 cgacaggcgt gccattacgc cgtaaacgta gaggcggttg tgttcgttgt ccacgtcgca
    14521 gtctttttca gagcgcggca tacccagcga gtcccattgt tcgaatacgc gcttgcaggc
    14581 ttcgggagct tcttgggagt tgtcgccgtt gccggtgggg atgctgttgg acagcggaac
    14641 aaacaccata ccgccggcgt tgaggttttc gtccgcaccg cgcccttcgt gtacgcggaa
    14701 aaagccgccg atgacgaagc ggtccatcat atagacgacg ggttcgcaca ccgcgccgtt
    14761 taaggtttca taagtataaa tcccttcttg gacaatcact tcgctgactt ccaagccttc
    14821 tttgactttc gccattttat tgcggttttt acggttcaat ccgcgcactt cgtcggcgga
    14881 tttgacgctc atcacgccca tgccgtaagt gccggcatcg gctttgacga tgacgaaagg
    14941 tttgtcggta atgcccgatt cgtcgtattt ggcttgaatt ttcgccagca cacgttctac
    15001 cgcttccgcc aacgcgtctt cgccttcacg cccttggaag tccaaaccgc cgattttttc
    15061 aaaataaggg ttaatttgcc attcgtcgat gccgattaac ttggcaaatt cggcggtaac
    15121 ttggttgtac gcgccgaaat gatttgtttt gcggcgcgtc gtccaaccgc cgtgcaacgg
    15181 cggcaaaacg gtttggctga tgcctttgag gatgtcggga atgccggcag acaaatcgtt
    15241 gttcaacaaa accacgcaag gcgaaaagcc gtctgcaaga tggacgcggt cgcgggtacg
    15301 caataaaggt tccaacagga ttttgtcgcc caatgcagtt tcaaattcgg tcggttcggt
    15361 tacttccgga ttcaggctgc ccaagcgcac ttcataccct gccgaacgca aaatctcgcc
    15421 gagggcgtaa atgttttgca ggtaaaacgt attgcgcgtg tggttttcgg gaataatcaa
    15481 tacggatttc gccgtttcgc aggcgcgttg caccgcatct tgcgcggcaa ccgccgccag
    15541 cgggataaag ttgggattca gattattgaa gccgccgggg aacaaattca tatcgataga
    15601 cgaaattttg taaccggcat tgcgtatatc gaccgaaccg taaaacggcg ggcggtgtac
    15661 gctccactgc gtgcggaacc acgcctcgat tttggcgtga ttggacagga tttttgcctc
    15721 aaacgcctga agttgcgccg aatgttcggg cgacatcacc ggtaatttca tctttcgtct
    15781 gcctctgtgt gttttttgac aagatgcaga tgataatgcc tttgaaagaa tgacgacaag
    15841 tattctttcg tatcgaatca tgctttagat ataactgttt actgaaatgg acaatacgtc
    15901 taattttttc gttaaaattg tttgcaaaat aacaccggct caaaaattag acaaaatttg
    15961 ttgcgcggta taaagaatac gtctaaaatc cgcccaatcc gcattaccat ttatccaaaa
    16021 gaacaacatc atgacagcac aaaccctcta cgacaaactt tggaacagcc acgtcgtccg
    16081 cgaagaagaa gacggcaccg tcctgctcta catcgaccgc catttggtgc acgaagttac
    16141 cagccctcag gcatttgaag gcttgaaaat ggcggggcgc aagctgtggc gcatcgacag
    16201 cgtcgtctcc accgccgacc acaacacccc gaccggcgat tgggacaaag gcatccaaga
    16261 cccgatttcc aagctgcaag tcgatacttt ggacaaaaac attaaagagt ttggcgcact
    16321 cgcctatttt ccgtttatgg acaaaggtca gggcatcgta cacgttatgg gccccgaaca
    16381 aggcgcgacc ctgcccggta tgaccgtcgt ctgcggcgac tcgcacactt ccacccacgg
    16441 cgcattcggc gcactggcgc acggcatcgg cacttccgaa gtcgagcaca cgatggcgac
    16501 ccagtgcatt accgcgaaaa aatccaaatc catgctgatt gccgttgacg gcaaattaaa
    16561 agcgggcgtt accgccaaag acgtggcact ttacatcatc gggcaaatcg gcacggcggg
    16621 cggtacaggc tacgccgtcg agtttggcgg cgaagccatc cgcagccttt ctatggaagg
    16681 ccgcatgact ttatgcaata tggcgatcga agccggtgcg cgctcaggca tggttgccgt
    16741 cgaccaaacc accatcgact atgtaaaaga taaacccttc gcacccgaag gcgaagcgtg
    16801 ggacaaagcc gtcgagtact ggcgcacgct ggtgtctgac gaaggtgcgg tattcgacaa
    16861 agaataccgt ttcaacgccg aagacatcga accgcaagtc acttggggta cctcgcctga
    16921 aatggtttta gacatcagca gcaaagtgcc gaatcctgcc gaagaaaccg atccggtcaa
    16981 acgcagcggt atggaacgcg cccttgaata catgggcttg aaagccggta cgccattaaa
    17041 cgaaatcccc gtcgacatcg tattcatcgg ctcttgcacc aacagccgca tcgaagactt
    17101 gcgcgaagcc gccgccatcg ccaaagaccg caaaaaagcc gccaacgtac agcgcgtgtt
    17161 aatcgtcccc ggctccggtt tggttaaaga acaagccgaa aaagaaggct tggacaaaat
    17221 tttcattgaa gccggttttg aatggcgcga accgggctgt tcaatgtgtc tcgccatgaa
    17281 cgccgaccgc ctcgcaccgg ggcaacgctg cgcctccacc tccaaccgca attttgaagg
    17341 ccgtcaaggc aacggcggac gtacccacct cgtcagcccc gctatggcag ccgccgccgc
    17401 cgttaccggc cgctttaccg acatccgcat gatggcgtaa gtattttcag gcggtcttta
    17461 agaatttgac gcggccgtct gaaatatatc ccgtaaggca gaaacacctt taatattcaa
    17521 accaaggagt ttaaccatga acaaactttt cattaccgcc ctgtccgccc tcgccttgtc
    17581 cgcctgcgcc ggcacttggg agggcgcgaa acaagacacc gcccgcaacc ttgacaaaac
    17641 acaggccgcc gccgaacgcg ccgccgaaca aacaggcaac gccgtcgaaa aaggctggga
    17701 caaaaccaaa gaagccgtca aaaaaggcgg caatgccgtc ggacgcggca tttcccatct
    17761 cggcggaaaa atcgaaaacg ccaccgaata accgattcgc gatgccgtct gaaaccgctt
    17821 cagacggcat cgtcagcaca agacgcaaac ccatgaaagc ctttaccaaa attaccgcca
    17881 tcgtcgcccc gctcgaccgc agcaacgtcg ataccgacgc catcatcccc aaacaatttt
    17941 taaaatccat caaacgcagc ggcttcggcc ccaatgcctt tgacgaatgg cgttacctcg
    18001 accacggcga accaggcatg gacaacagca aacgcccgtt gaaccctgat ttctcgctga
    18061 accagccgcg ttaccaaggc gcgcaaatcc tgttgacgcg taaaaacttc ggttgcggct
    18121 cttcacgcga acacgcccct tgggcattgg acgactacgg cttccgcgcc gtcatcgccc
    18181 ccagcttcgc cgacatcttc tttaacaact gctacaaaaa cggcctttta cctatcgtgt
    18241 tgaccgaaga acgggttgac cgacttttca aagaagttga agccaacgaa ggctatcagc
    18301 tctccatcga ccttgccgaa caaaccctga ccacaccaag cggcgaaacg ttcacatttg
    18361 acattaccga acaccgcaaa cactgcctct taaacggctt ggacgaaatc ggtctgaccc
    18421 tgcaacacgc cgacgaaatt cacgcctttg aagaaaaacg ccgccaaagc cagccttggc
    18481 tgtttaacgg ttaaaaccta taccgtctga aggccgttca gacggcattc ctgataacat
    18541 ataattgttg cggctaatgt aaacagaggg gcgataaatt gctttcatct tttaagcggg
    18601 cttagcgaca ctctaaatta atgcaataat gcaactctcc atttcaaata aatttttaga
    18661 tttttaatat gcaacaaatt aaatttattg acttattttc cgaaatgagt ggcatcagga
    18721 aaggattcga acaagcctgt cggaaacagt cggttgcttg cgaatgtgtt tttacttcag
    18781 aaatcaaacc ggcagctttg gaagtattga agcaaaacta ccctgatgaa gtgccgtatg
    18841 gagatataac gaaaattgaa acaggggata ttcccgattt tgacatcctg ttggcaggct
    18901 tcccttgtca ggctttttct ttcgcctgaa aaagattagg ctttgaagat acacggggaa
    18961 cgcttttctt tgatgtggca aggattttaa aggcaaaaaa accaaaaggt tttattttgg
    19021 aaaatgtgga aggattggtg acacacgata gaaaagattc gacacaaaaa atagggcgca
    19081 ccctgaccgt tattttggaa accttggaag cattgggcta ctatgtttct tggaaagttt
    19141 taaatgcaaa agatttcggc attccccaaa acaggaagcg catttatctg acaggcagtt
    19201 tgaaatccaa acccgactta tctttcgaaa caagcccaag tccgaaatta aaaaatattt
    19261 tggaatcggg actgcctacc gaaagcagcc ctttcatcaa aaaattgcta aaaaaatttc
    19321 ccccgtccga actgtacgga aaatcagtaa aagacaaacg gggagggaaa aacaatattc
    19381 acagttggga tattgaatta aaaggcgcag taaccgaaga agagaagcaa ttgttaaata
    19441 tccttctaaa agaacggagg aaaaaaaatg ggcttcagaa atcggcatag attggatgga
    19501 tgggatgcct ttgacaaaag cgcaaatttc aactttctat aaacaccccg atctccaaaa
    19561 tattttggac agcctgacag ataaaggcta tttggtttta gagcatccga aacaaaaaat
    19621 tggcgggcaa agaatcaaag atgaatccct gcccaaaggc tacaatattg tttcaggcaa
    19681 aaaatctttt gaaatcaata aaatattaga tcaaaacgat gttgcgccaa ccttggttgc
    19741 aatgaatatg gagcaccttt tcgtcgttga caacggcggt ttgcgtacac ttaccggaaa
    19801 agaagggttg cgcttattcg gctatccgga cgattattcg tttgatattc ccaaaaaaga
    19861 cagatgcgat ttattgggta ataccgttgc cgtccctgtg attaaggcgg tatctgaaag
    19921 acttctgcat actttataaa ccgacaggga taaaatgata aaacttactg cacaacaaat
    19981 atttgataag ttgctggatg aagaaaaaat cttatcggcc aatggtcaaa ttagattttt
    20041 cttaggcgat gtggatatta tcgtcaaaca aaaagatgtt gtcggcaaca tcattcagga
    20101 atggctcggc ggatgattga gaaaaaggga aattgaattt gatgtttcaa ccaataccca
    20161 aatgcctccc gatttctttt taaataaaaa agaccgcagc agagaattgt tggaagtaaa
    20221 ggcgttcaac cgaaatgccg gcccgggttt tgatattgca gattttaaaa tgtattctga
    20281 tgaaatcatt cataagccct atatgctgga tgtagactat ttaatattcg gttacgatat
    20341 ggacgacaac ggcaatgtaa ccatcaagga tttatggctt aaaaaagtat ggcaaattac
    20401 gagaagcatg gatgggtggg caattaatct tcaagtcaaa aaaggcgtgg tgcataaaat
    20461 ccgcccgggt gtttggtaca gcataaataa aaagaatatg cctatgtttg aatgcttgga
    20521 agattttgtt tccgcaattg aagaaaccgt ttatcaaaac ccggcaacac ggcataacgc
    20581 atccttatgg aaaaagaaat ttgaggaagc ctataaaaaa cactacaacc gatcgatttc
    20641 catacctcgt tggcacgaaa ttgcacacaa atacaaaaag aaataaccct tcaaccatca
    20701 acccataagg aaacttgaaa tgaccaaaca tatcgccatc ctccgaggtg acggcatcgg
    20761 cccagaaatc gtcgccgaaa ccgtccgcgt actcgacaaa ctcatcgccc aaggcttgga
    20821 cgccagctac gaatacgccc cattaggcgg cgaagcctac gacgaatacg gccatcccta
    20881 tccccaattc acgcaaaacc tttgccgtaa agccgatgcc gtcctgcttg gtgcagtcgg
    20941 ttctcctcaa tacgacaatc tcgaccgtcc attgcgtcct gagcgcggct tgttggccat
    21001 acgtaaagac ttgaatctgt ttgccaattt gcgccctgct atcctctatc ccgagctagc
    21061 caacgcctcc acgctcaagc ctgaaatcgt cgccggtttg gatattttga ttgtgcgcga
    21121 actcaccggc gatatttatt tcggcgaacc gcgcggcatc cgtgttttag aaaacggcga
    21181 acgcgaaggc tacaacacca tgaaatacag cgaaagcgaa atccgccgta ttgcccacgt
    21241 tgccttccaa tccgcccaaa aacgcagcaa aaaagtctgc tccgtcggca aagccaacgt
    21301 tttggaaacc accgaattgt ggcgcgaaat ctttgaggaa atcggcaaag aataccccga
    21361 tgtcgagctt tcccatatgt acgttgataa cgcagccatg cagctcgtgc gcgcgcccaa
    21421 acaatttgac gtgattgcca ccggcaacat cttcggcgat attttatcgg acgaagcctc
    21481 tatgctgacc ggctccatcg gtatgctgcc ttccgcttct ttggacgaaa acggcaaagg
    21541 cctgtacgaa ccgtcccacg gctccgcccc cgacatcgcc ggacaaaaca aagccaaccc
    21601 gcttgccacc atcctctcgc tcgcaatgtt gctgcgttac agcctgaacg acgaagcgcg
    21661 cgcgcaacaa gttgaaaacg ccgtacaaaa agtgctgcaa caaggcttgc gcaccggcga
    21721 tatttacgaa gaaggcacga aactcgtttc ctgctccgaa atgggtgatg cggtactcgc
    21781 cgccttgtaa aaaaacaaag cgcggatcac accgcgcttt ttcatccgcc cttttccgcc
    21841 cgccccgttc gtcaaccgcc tgcaaaccca ccgtttaccg gcaggaaatg ccataaatcc
    21901 ctgcctatgt taggataaac gcctttaccg gcccacacaa aggattatca tcatgaaaaa
    21961 aatcatcatc gccgcgctcg cagccgccgc catcggcact acctccgccg ccacctacaa
    22021 agtggacgaa tatcacgcca acgcccgttt cgccatcgac catttcaata ccagcaccaa
    22081 cgtcggcggt ttttacggtc tgaccggttc cgttgagttc gaccaagcaa aacgcgacgg
    22141 taaaatcgac atcaccattc ccgttgccaa cctgcaaagc ggttcgcaac actttaccga
    22201 ccacctgaaa tccgccgaca tcttcgatgc cgcccaatat cctaacatcc gcttcgtttc
    22261 caccaaattc aacttcaacg gcaaaaaact ggtttccgtt gacggcaacc tgaccatgca
    22321 cggcaaaacc gcccccgtca aactcaaagc cgaaaaattc aactgctacc aaagcccgat
    22381 gttgaaaacc gaagtttgcg gcggcgactt cagcaccacc atcgaccgca cccaatgggg
    22441 cgtggactac ctcgttaacg ttggtatgac caaaagcgtc cgcatcgaca tccaaatcga
    22501 ggcagccaaa caataaatct tgatgcgaat cagcacaaat gccgtctgaa cgtccttcaa
    22561 acggcatttg tgccgtcttc atcaaaccgc cccgccaacg gtaagccgat atggcggagc
    22621 acaatccgcc gaaagatatt tcagcggcag gcaggacttg ccggaaagtc cggaaagtat
    22681 agt
//
LOCUS       DAWISC010000012        22145 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWISC010000012 DAWISC010000000
VERSION     DAWISC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.012"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(246..1289)
                     /locus_tag="WIW87_000336"
     CDS             complement(246..1289)
                     /locus_tag="WIW87_000336"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ5173552.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFVDI
                     LPTGSAPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLDAIPLLP"
     gene            complement(1286..1567)
                     /locus_tag="WIW87_000337"
                     /pseudo
     CDS             complement(1286..1567)
                     /locus_tag="WIW87_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1822..2976
                     /locus_tag="WIW87_000338"
     CDS             1822..2976
                     /locus_tag="WIW87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ5173553.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3205..3558
                     /locus_tag="WIW87_000339"
     CDS             3205..3558
                     /locus_tag="WIW87_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173554.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3619..4335
                     /locus_tag="WIW87_000340"
     CDS             3619..4335
                     /locus_tag="WIW87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ5173555.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4400..4843
                     /locus_tag="WIW87_000341"
     CDS             4400..4843
                     /locus_tag="WIW87_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ5173556.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADILHEKR"
     gene            complement(5092..5199)
                     /locus_tag="WIW87_000342"
                     /pseudo
     CDS             complement(5092..5199)
                     /locus_tag="WIW87_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5209..5511
                     /locus_tag="WIW87_000343"
     CDS             5209..5511
                     /locus_tag="WIW87_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173557.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5795..6034
                     /locus_tag="WIW87_000344"
     CDS             5795..6034
                     /locus_tag="WIW87_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ5173558.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            6095..7837
                     /gene="hrpB"
                     /locus_tag="WIW87_000345"
     CDS             6095..7837
                     /gene="hrpB"
                     /locus_tag="WIW87_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ5173559.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7946..13882
                     /gene="hrpA"
                     /locus_tag="WIW87_000346"
     CDS             7946..13882
                     /gene="hrpA"
                     /locus_tag="WIW87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ5173560.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGPATLSADGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLTAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAAIVTIIVTALTYGYG
                     ATAAGGVAASGSSTAAAAGTAATTTAAATTVSTATAMQTAALASLYSQAAVSIINNKG
                     DVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANL
                     GGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDY
                     VAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVADSMLGGRNPATLSDAEKHKVISY
                     SKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKII
                     QGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKL
                     AISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQT
                     VKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIAN
                     NRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTT
                     IKVFTDKSGNLITTYPVKGN"
     gene            13889..14398
                     /locus_tag="WIW87_000347"
     CDS             13889..14398
                     /locus_tag="WIW87_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173561.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            14458..16146
                     /locus_tag="WIW87_000348"
     CDS             14458..16146
                     /locus_tag="WIW87_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5173562.1"
                     /translation="MRSMIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLF
                     EAPRLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVN
                     WNQVQLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFA
                     SLASQASVSLINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETP
                     WVNNLNVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKGLDQHY
                     VAHKIAHAVAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            16155..16532
                     /locus_tag="WIW87_000349"
     CDS             16155..16532
                     /locus_tag="WIW87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173563.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            16810..17184
                     /locus_tag="WIW87_000350"
     CDS             16810..17184
                     /locus_tag="WIW87_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173564.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            17219..19171
                     /locus_tag="WIW87_000351"
     CDS             17219..19171
                     /locus_tag="WIW87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5173565.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMV
                     AVATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIV
                     GEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVNAAANAAEVAVKNNQLSD
                     KEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTDISRSTECRTIR
                     KQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNTAAKSWLQSGNT
                     KPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISFDTNLKRHLANA
                     DGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEIPTLDRTGKPDG
                     GFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTKSISERKNVIQF
                     SETFDGIKFRSYFDVNTGRITNIHPE"
     gene            19187..19618
                     /locus_tag="WIW87_000352"
     CDS             19187..19618
                     /locus_tag="WIW87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173566.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            19763..>22145
                     /locus_tag="WIW87_000353"
     CDS             19763..>22145
                     /locus_tag="WIW87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5173567.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTSRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKL"
BASE COUNT         6679 a         4999 c         5216 g         5251 t
ORIGIN      
        1 aaaaaccacg gtgttatcgg caatgactga aaccggagaa atgacggaat cgaagcgtgt
       61 ggaaatgtgt cggaaattgc tgaagctgga taaactagat tcccgctttt gcgggaatga
      121 cggtatttta gatttttgtt ttcgtgtgaa tggatgggtc aaaagccgtc cgaacccaaa
      181 aaaacgtcgc tttcagacgg catcaaaaac cgcaggcacg atgcctgcgg tttgctgttt
      241 gcgcttcagg ggagcagggg gattgcgtcc aagccgtgtg tttcctccag tccgaacata
      301 atgttcatat tttggactgc ctgacccgcc gcgcctttga cgaggttgtc gatgacggaa
      361 aggacgaccc acacatcgga ttgcgccgcc tgttggatgc tgatgcggca gaggtttgcg
      421 ccgcgcacgc tgcgggtttc gggggcggaa ccggtcggca ggatgtccac gaacgggctg
      481 tcgcggtagt agtcgcgcag gacggtttcg gggtcgctgc cgtctgaaag gtggaggtaa
      541 acggtggcgt gcataccgcg tatcattggc gcgaggtgcg gcgtgaacac gaatccttcg
      601 gcgatgccgt cctgaagccc ggcgatggtc tgcctgattt cgggcaggtg gcggtgtccg
      661 gctatgccgt aggctttgaa gttgtcgccg gcttcgcaca acagcgaacc gacattgcct
      721 ttcctgcccg cgccggacac accggatttg cagtcggcaa tcagcggcat accgggcttc
      781 agacggcatt gccgcaacag cggcacgagc ggtagggata cgcaggtcgg gtagcagccg
      841 gggttggcga cgaggcgcgc ctgtgcgacg gcttcgcggt tgagttcgct caatccgtac
      901 acggcttggg aaacgaggtc gggggcggcg tgggtcatgc cgtaccagtg ttcccaggtc
      961 ggaatgtccc gtatgcggaa gtcggcggaa aggtcgatga cgcgcacgcc ctgttcaatc
     1021 aggcgcggcg cgtctttcat ggcgatgccg ttgggcgtgg cgaagaagac gatgtcgcat
     1081 tgttccaaac ctgcctcgtc gggcgtttgg aaggcgaggc cgtacacgcc gcgcaaactc
     1141 ggaaagtaat cggcaactgc ggttcccgct tcgctgcggc tggttacggc ggcgacttcg
     1201 acatcgggat gggcggcaag caggcgcagc agttccacgc cggtgtagcc cgtcgcgccg
     1261 acaatgccga ctttgatttt tttactcatg gtttttcctt tgtgtggttg gcgggtatgc
     1321 cgtccgaacg ctgtccgacg gggttttgat tggcggcgga aggacggtac acaaagcgtt
     1381 tgaaaagcgt gtctaattta atggcatccg cctctgtttt taaaacctag ccctgccgtc
     1441 cggaataaac ataaccgtag gcgcgaaagc cggcaggata tttcggtgcc tgccccaaac
     1501 ccaaaccgta tgcggcgaaa gcggtggtcg tggagaagga aagcaggagg aggacgaggg
     1561 ttttcataag gttccaaacc gaacggggcg atgaagcccg attatagcaa aaggtcgtct
     1621 gaaaaccgtt ttcagacgac cttttattta attacaataa aaatgttaac aacaaaaaac
     1681 aaaccgcttt tttccgtttg gagacaaatt tttaactaaa attgtcaaca attccttgac
     1741 gcacacacaa aaaaaaactt taatatttgt aaatatatgt aacattataa attttaaata
     1801 ttttctgagt aaggggaagt aatgagcatc catctcgact tcggcattag tcctaaaacg
     1861 ttccgacaga cttatctgta tcaaaagccc aagctcttta aaggagcggt tcggaatctc
     1921 gaatccgcat cttggaaaga tatcaacgag atataccaac gagcagaccc aaccgcaccg
     1981 ctgtttcatc tgcgtaaaaa aggcgcaatc gttcctaaag aagaatacgt cgaaagtttc
     2041 gacgatttgg gcaaaactcg ctaccgtttt attaaatccg ttatctacga acatatgaag
     2101 aatggtgcgt cgttagtcta taaccatatt aacaacgagc cgttttcaga ccatatcgcc
     2161 cgtcaagtcg cccgctttgc cggcgcacat actattgtta gtggatatct tgcttttggc
     2221 agcgacgaat cttataaaaa ccattgggac ccccgcgatg tgtatgccat ccagcttttc
     2281 ggcaagaaac gttggcaact tactgcccct gatttcccta tgccattgta tatgcaacag
     2341 actaaagata ctgatatttc cattcctgaa catatcgata tggatattat ccttgaagca
     2401 ggtgatgtcc tctacatccc acgcggttgg tggcacagac ctatcccgct cggctgtgaa
     2461 accttccact tcgctgtcgg tacctttcca cccaacggct ataattacct cgagtggcta
     2521 atgaagaaat ttcccaccat agaaagtctg cgccacagtt tctcagactg ggagcaagat
     2581 aggacgcgta tcaacgatac tgccgcacaa attgctgcca tgattgccga ccccgtcaat
     2641 tatgaagcct tcagtgaaga ctttctcggc aaagaacgta ccgataccgc ttttcatctc
     2701 gaacagttcg cgaatcccaa cgctactccg ctttcagacg acgtcaggtt gagattaaat
     2761 gccaataatt tggatacgtt ggaaaaggga tatttgattg ggaatgggat gaagataagc
     2821 gtagatgagt tggggaaaaa agtgttagaa cacatcggta agaatgaacc gttattgttg
     2881 aaaaatctac tggttaactt caatcaggga aaatatgaag aagttaggaa gttgatttat
     2941 cagttgatag agttagattt tctggaaatt ttgtgaggga ttctatgaaa aactggaagc
     3001 aatttacatt tttcgtaata ttagtaatag cttgttatca agtgctatat tttttatcag
     3061 atatgtttct tctcgactac attaacaaat atagttggaa tttgaatttt attcagggta
     3121 ctttgaattt tttttcaata tatctgccat atgtttttgt tagccgaatt tttaggaata
     3181 ccaaccaaga aaaggagtat aaaaatgatt gaacttcaac ttcatgaatt gaagctggtt
     3241 tcagggggag gtcctgtaac agacaatata gctggaaatg tagctaatgc tgccacaacc
     3301 aaaggaggtc ccacatgggg ggatttggtt gcaatacctg ctgccgcagc ggcagtttat
     3361 tatctgccga aaaatgctta cggtgctgct ggtgcaaatg gtgtatacaa tgtgactcgt
     3421 aattgggtaa atgatgccgt taatgcacct ccttataacg gaagaccaat ctttgagatt
     3481 gaacatggat taactgctcc cgcaacaaaa gcagataaat caggaaacgg ctacactgac
     3541 ggtacagatt actgctgata tttctcatcg tccagacaat ctctaggggt cgtctgaaac
     3601 tttcttaact tcaattttat gaatagaccc aagcaaccct tcttccgtcc cgaagtcgcc
     3661 gttgcccgcc aaaccagcct gacgggtaaa gtgattctga cacgaccgtt gtcattttcc
     3721 ctatggacga catttgcatc gatatctgcg ttattgatta tcctgttttt gatatttggt
     3781 aactatacgc gaaagacaac agtggaggga caaattttac ctgcatcggg cgtaatcagg
     3841 gtgtatgcac cggatacggg gacaattaca gcgaaattcg tggaagatgg agaaaaggtt
     3901 aaggctggcg acaagctatt tgcgctttcg acctcacgtt tcggcgcagg aggtagcgtg
     3961 cagcagcagt tgaaaacgga ggcagttttg aagaaaacgt tggcagaaca ggaactgggt
     4021 cgtctgaagc tgatacacgg gaatgaaacg cgcagcctta aagcaactgt cgaacgtttg
     4081 gaaaaccagg aactccatat ttcgcaacag atagacggtc agaaaaggcg cattagactt
     4141 gcggaagaaa tgttgcagaa atatcgtttc ctatccgcca atgatgcagt gccaaaacaa
     4201 gaaatgatga atgtcaaggc agagctttta gagcagaaag ccaaacttga tgcctaccgc
     4261 cgagaagaag tcgggctgct tcaggaaatc cgcacgcaga atctgacatt ggccagcctc
     4321 ccccaagcgg catgagacag aacaaagctg gcttgaacgc accattgccg atatttctca
     4381 agaagttttg gattttgaaa tgcgctctga acaaatcatc cgtgcaggac ggtcgggtta
     4441 tatagcaata ccgaacgtcg aagtcggaca gcaggttgat ccttccaaac tgctcttgag
     4501 cattgttccc gaacgtaccg agctatatgc ccatctatat atccccagca gtgcagcagg
     4561 ctttatcaag ccgaaagaca aggttgtcct acgttatcag gcatatccct atcaaaaatt
     4621 cgggcttgct tccggcagtg tcgtatcagt agcaaaaacg gcactgggca gacaggaatt
     4681 gtcgggattg ggcatggtat cctccgattt ggcgaagagc aacgaacctg tttatcttgt
     4741 gaaaataaaa cccgacaaat caaccatcac tgcatacggt gaggaaaaac cgctgcaaat
     4801 cggcatgacg ttggaagcag acatcctaca cgagaaacgg tgattgtacg aatgggtatt
     4861 ggagctgatt tatagtatgt cgggcaaact gtaagacttg aggctgtaaa gataaagaag
     4921 ttgtctgaaa ccttttcaga cgaccagaag acaatatata aaaacacaaa cttcaaaact
     4981 gcctgaaacc atcccctttt acaggcactc cgtttgtaaa agcaaggata gggcatctgc
     5041 ccttttgatt gtttgattaa cgatacaagg agtttcaaaa tgagagtttt atagtggatt
     5101 aaatttaaac cagtacagcg ttgcctcgcc ttgccgtacc atttgtactg tctgcggctt
     5161 cgtcgccttg tcctgattta aatttaatcc actatatgtg ttcatgaaat gacttgggtc
     5221 ggaggctcag gtaatgcgca acaaagctca tattattgcg aaatttgcga atctgcaggg
     5281 cttaacgata cgggaaatcc tgataaatct ttaggattgc caaacaatac gttcagtaat
     5341 ccgcctggtt ggggagctac aatcggagct ttagcaggta gccgcatagg gatgcctgaa
     5401 tttggtacgt ttgcgagcca tgccattgaa aatttcgact ggtcatggta tcgacgttat
     5461 agggaaattg ccgaaacgat tgaacgagaa tattcaggcg gtttgcctta atagttgagg
     5521 aggtcatgat gtttgccaaa cattatcaat tcatcgcact cggcatcatg ctgcttcttt
     5581 atatgttgat tctctatacg accgattttt ccaatctgac gtattggatg ctgtttttta
     5641 tctgttttat tacaggaaaa atattagctc gtttgttaga gaaaagcttt aaataaaata
     5701 gcagctagtc gcaaaaggtc gtctgaaacc ttttcaggcg gcctttctaa aatacatcca
     5761 acttcctaat ccctattttt caaaaaggaa atctatgccc catctgcaaa acctgtcttt
     5821 gggcttaaag aaaaagctgc ctgttatcct gcaaacagaa atatcagaat gcggcttggc
     5881 atgtctggcg gctgtggcgg gatttcatgg tttccatacg aatttacgcg cactgcgttc
     5941 aaaatactgt ccgagacctt tgcaaaattc cccaaaatcc cctaaatgtc ttggtgggaa
     6001 ttttggggaa ttttgcaaag gtctcattct ataactgtaa atacttttaa atttatgaca
     6061 aaatagtaaa tattgctaaa ataatattga tgtcatgaaa ttttttcctg ctccatgtct
     6121 gttggttatc ctggctgtca taccccttaa aaccttagct gccgatgaaa acgatgcaga
     6181 acttatccgt tccatgcagc gtcagcagca catagatgct gaattgttaa ctgatgcaaa
     6241 tgtccgtttc gagcaaccat tggagaagaa caattatgtc ctgagtgaag atgaaacacc
     6301 gtgtactcgg gtaaattaca ttagtttaga tgataagacg gtgcgcaaat tttcttttct
     6361 tccttctgtg ctcatgaaag aaacagcttt taaaactggg atgtgtttag gttccaataa
     6421 tttgagcagg ctacaaaaag ccgcgcaaca gatactgatc gtgcgtggct acctcacttc
     6481 ccaagctatt atccaaccac agaatatgga ttcgggaatt ctgaaattac gggtatcagc
     6541 aggcgaaata ggggatatcc gctatgaaga aaaacgggat gggaagtctg ccgagggcag
     6601 tattagtgca ttcaataaca aatttccctt atataggaac aaaattctca atcttcgcga
     6661 tgtagagcag ggcttggaaa acctgcgtcg tttgccgagt gttaaaacag atattcagat
     6721 tataccgtcc gaagaagaag gcaaaagcga tttacagatc aaatggcagc agaataaacc
     6781 catacggttc agtatcggta tagatgatgc gggcggcaaa acgaccggca aatatcaagg
     6841 aaatgtcgct ttatcgttcg ataacccttt gggcttaagc gatttgtttt atgtttcata
     6901 tggacgcggt ttggcgcaca aaacggactt gactgatgcc accggtacgg aaactgaaag
     6961 cggatccaga agttacagcg tgcattattc ggtgcccgta aaaaaatggc tgttttcttt
     7021 taatcacaat ggacatcgtt accacgaagc aaccgaaggc tattccgtca attacgatta
     7081 caacggcaaa caatatcaga gcagcctggc cgccgagcgc atgctttggc gtaacagact
     7141 tcataaaact tcagtcggaa tgaaattatg gacacgccaa acctataaat acatcgacga
     7201 tgccgaaatc gaagtacaac gccgccgctc tgcaggctgg gaagccgaat tgcgccaccg
     7261 tgcttacctc aaccgttggc agcttgacgg caagttgtct tacaaacgcg ggaccggcat
     7321 gcgccaaagt atgcctgcac cggaagaaaa cggcggcgat attcttccag gtacatctcg
     7381 tatgaaaatc attactgccg gtttggacgc agccgcccca tttattttag gcaaacagca
     7441 gtttttctac gcaaccgcca ttcaagctca atggaacaaa acgccgttgg ttgcccaaga
     7501 taaattgtca atcggcagcc gctacaccgt tcgcggattt gatggggagc agagtctttt
     7561 cggagagcga ggtttctact ggcagaatac tttaacttgg tattttcatc cgaaccatca
     7621 gttctatctc ggtgcggact atggccgcgt atctggcgaa agtgcacaat atgtatcggg
     7681 caagcagctg atgggtgcag tggtcggctt cagaggaggg cataaagtag gcggtatgtt
     7741 tgcttatgat ctgtttgccg gcaagccgct tcataaaccc aaaggctttc agacgaccaa
     7801 caccgtttac ggcttcaact tgaattacag tttctaacct ctgaattttt tactgatatt
     7861 tagacggtct ttccttatcc tcagaccgtc aaactttacc tacgtacttg gcgcgcagta
     7921 cgttcatctt caaaatggaa tagacatgaa taaaggttta catcgcatta tctttagtaa
     7981 aaagcacagc accatggttg cagtagccga aactgccaac agccagggca aaggtaaaca
     8041 ggcaggcagt tcggtttctg tttcactgaa aacttcaggc gacctttgcg gcaaactcaa
     8101 aaccaccctt aaaactttgg tctgctcttt ggtttccctg agtatggtat tgcctgccca
     8161 tgcccaaatt accaccgaca aatcagcacc taaaaaccag caggtcgtta tccttaaaac
     8221 caacactggt gcccccttgg tgaatatcca aactccgaat ggacgcggat tgagccacaa
     8281 ccgctatacg cagtttgatg ttgacaacaa aggggcagtg ttaaacaacg accgtaacaa
     8341 taatccgttt gtggtcaaag gcagtgcgca attgattttg aacgaggtac gcggtacggc
     8401 tagcaaactc aacggcatcg ttaccgtagg cggtcaaaag gccgacgtga ttattgccaa
     8461 ccccaacggc attaccgtta atggcggcgg ctttaaaaat gtcggtcggg gcatcttaac
     8521 taccggtgcg ccccaaatcg gcaaagacgg tgcactgaca ggatttgatg tgcgtcaagg
     8581 cacattgacc gtaggagcag caggttggaa tgataaaggc ggagccgact acaccggggt
     8641 acttgctcgt gcagttgctt tgcaggggaa attacagggt aaaaacctgg cggtttctac
     8701 cggtcctcag aaagtagatt acgccagcgg cgaaatcagt gcaggtacgg cagcgggtac
     8761 gaaaccgact attgcccttg atactgccgc actgggcggt atgtacgccg acagcatcac
     8821 actgattgcc aatgaaaaag gcgtaggcgt caaaaatgcc ggcacactcg aagcggccaa
     8881 gcaattgatt gtgacttcgt caggccgcat tgaaaacagc ggccgcatcg ccaccactgc
     8941 cgacggcacc gaagcttcac cgacttatct ctccatcgaa accaccgaaa aaggagcggc
     9001 aggcacattt atctccaatg gtggtcggat cgagagcaaa ggcttattgg ttattgagac
     9061 gggagaagat atcagcttgc gtaacggagc cgtggtgcag aataacggca gtcgcccagc
     9121 taccacggta ttaaatgctg gtcataattt ggtgattgag agcaaaacta atgtgaacaa
     9181 tgccaaaggc ccggctactc tgtcggccga cggccgtacc gtcatcaagg aggccagtat
     9241 tcagactggc actaccgtat acagttccag caaaggcaac gccgaattag gcaataacac
     9301 acgcattacc ggggcagatg ttaccgtatt atccaacggc accatcagca gttccgccgt
     9361 aatagatgcc aaagacaccg cacacatcga agcaggcaaa ccgctttctt tggaagcttc
     9421 aacagttacc tccgatatcc gcttaaacgg aggcagtatc aagggcggca agcagcttgc
     9481 tttactggca gacgataaca ttactgccaa aactaccaat ctgaatactc ccggcaatct
     9541 gtatgttcat acaggtaaag atctgaattt gaatgttgat aaagatttgt ctgccgccag
     9601 catccatttg aaatcggata acgctgccca tattaccggc accagtaaaa ccctcactgc
     9661 ctcaaaagac atgggtgtgg aggcaggctc gctgaatgtt accaatacca atctgcgtac
     9721 caactcgggt aatctgcaca ttcaggcagc caaaggcaat attcagcttc gcaataccaa
     9781 gctgaacgca gccaaggctc tcgaaaccac cgcattgcag ggcaatatcg tttcagacgg
     9841 ccttcatgct gtttctgcag acggtcatgt atccttattg gccaacggta atgccgactt
     9901 taccggtcac aataccctga cagccaaggc cgatgtcaat gcaggatcgg ttggtaaagg
     9961 ccgtctgaaa gcagacaata ccaatatcac ttcatcttca ggagatatta cgttggttgc
    10021 cggcaacggt attcagcttg gtgacggaaa acaacgcaat tcaatcaacg gaaaacacat
    10081 cagcatcaaa aacaacggtg gtaatgccga cttaaaaaac cttaacgtcc atgccaaaag
    10141 cggggcattg aacattcatt ccgaccgggc attgagcata gaaaatacca agctggagtc
    10201 tacccataat acgcatctta atgcacaaca cgagcgggta acgctcaacc aagtagatgc
    10261 ctacgcacac cgtcatctaa gcattaccgg cagccagatt tggcaaaacg acaaactgcc
    10321 ttctgccaac aagctggtgg ctaacggtgt attggcactc aatgcgcgct attcccaaat
    10381 tgccgacaac accacgctga gagcgggtgc aatcaacctt actgccggta ccgccctagt
    10441 caagcgcggc aacatcaatt ggagtaccgt ttcgaccaaa actttggaag ataatgccga
    10501 attaaaacca ttggccggac ggctgaatat tgaagcaggt agcggcacat taaccatcga
    10561 acctgccaac cgcatcagtg cgcataccga cctgagcatc aaaacaggcg gaaaattgct
    10621 gttgtctgca aaaggaggaa atgcaggtgc gcctagtgct caagtttcct cattggaagc
    10681 aaaaggcaat atccgtctgg ttacaggaga aacagattta agaggttcta aaattacagc
    10741 cggtaaaaac ttggttgtcg ccaccaccaa aggcaagttg aatatcgaag ccgtaaacaa
    10801 ctcattcagc aattattttc ctacacaaaa agcggctgaa ctcaaccaaa aatccaaaga
    10861 attggaacag cagattgcgc agttgaaaaa aagctcgcct aaaagcaagc tgattccaac
    10921 cctgcaagaa gaacgcgacc gtctcgcttt ctatattcaa gccatcaaca aggaagttaa
    10981 aggtaaaaaa cccaaaggca aagaatacct gcaagccaag ctttctgcac aaaatattga
    11041 cttgatttcc gcacaaggca tcgaaatcag cggttccgat attaccgctt ccaaaaaact
    11101 gaaccttcac gccgcaggcg tattgccaaa ggcagcagat tcagaggcgg ctgctattct
    11161 gattgacggc ataaccgacc aatatgaaat tggcaagccc acctacaaga gtcactacga
    11221 caaagctgct ctgaacaagc cttcacgttt gaccggacgt acaggggtaa gtattcatgc
    11281 agctgcggca ctcgatgatg cacgtattat tatcggtgca tccgaaatca aagctccctc
    11341 aggcagcata gacatcaaag cccatagtga tattgtactg gaggctggac aaaacgatgc
    11401 ctataccttc ttaaaaacca aaggtaaaag cggcaaaatc atcagaaaaa ccaagtttac
    11461 cagcacccgc gaccacctga ttatgccagc ccccgtcgag ctgaccgcca acggcataac
    11521 gcttcaggca ggcggcaaca tcgaagctaa taccacccgc ttcaatgccc ctgcaggtaa
    11581 agttaccctg gttgcgggtg aagagctgca actgctggca gaagaaggca tccacaagca
    11641 cgagttggat gtccaaaaaa gccgccgctt tatcggcatc aaggtaggca agagcaatta
    11701 cagtaaaaac gaactgaacg aaaccaaatt gcctgtccgc gtcgtcgccc aaactgcagc
    11761 cacccgttca ggctgggata ccgtgctcga aggtaccgaa ttcaaaacca cgctggccgg
    11821 tgcggacatt caggcaggtg taggcgaaaa agcccgtgcc gatgcgaaaa ttatcctcaa
    11881 aggcattgtg aaccgtatcc agtcggaaga aaaattagaa accaactcaa ccgtatggca
    11941 gaaacaggcc ggacgcggca gcactatcga aacgctgaaa ctgcccagct tcgaaagccc
    12001 tactccgccc aaactgaccg cccccggtgg ctatatcgtc gacattccga aaggcaattt
    12061 gaaaaccgaa atcgaaaagc tggccaaaca gcccgagtat gcctatctga aacagctcca
    12121 agtagcgaaa aacgtcaact ggaaccaggt gcaactggct tacgataaat gggactataa
    12181 gcaggaaggc ttaaccagag ccggtgcagc gattgttacc ataatcgtaa ccgcactgac
    12241 ttatggatac ggcgcaaccg cagcgggcgg tgtagccgct tcaggaagta gtacagccgc
    12301 agctgccgga acagccgcca caacgacagc agcagctact accgtttcta cagcgactgc
    12361 catgcaaacc gctgctttag cctccttgta tagccaagca gctgtatcca tcatcaataa
    12421 taaaggtgat gtcggcaaag cgttgaaaga tctcggcacc agtgatacgg tcaagcagat
    12481 tgtcacttct gccctgacgg cgggtgcatt aaatcagatg ggcgcagata ttgcccaatt
    12541 gaacagcaag gtaagaaccg aactgttcag cagtacgggc aatcaaacta ttgccaacct
    12601 tggaggcaga ctggctacca atctcagtaa tgcaggtatc tcagctggta tcaataccgc
    12661 cgtcaacggc ggcagcctga aagacaactt aggcaatgcc gcattaggag cattggttaa
    12721 tagcttccaa ggagaagccg ccagcaaaat caaaacaacc ttcagcgacg attatgttgc
    12781 caaacagttc gcccacgctt tggctgggtg tgttagcgga ttggtacaag gaaaatgtaa
    12841 agacggggca attggcgcag cagttgggga aatcgtagcc gactccatgc ttggcggcag
    12901 aaaccctgct acactcagcg atgcggaaaa gcataaggtt atcagttact cgaagattat
    12961 tgccggcagc gtggcggcac tcaacggcgg cgatgtgaat actgcggcga atgcggctga
    13021 ggtggcggta gtgaataatg ctttgaattt tgacagtacc cctaccaatg cgaaaaagca
    13081 tcaaccgcag aagcccgaca aaaccgcact ggaaaaaatt atccaaggta ttatgcctgc
    13141 acatgcagca ggtgcgatga ctaatccgca ggataaggat gctgccattt ggataagcaa
    13201 tatccgtaat ggcatcacag gcccgattgt gattaccagc tatggggttt atgctgcagg
    13261 ttggacagct ccgctgatcg gtacagcggg taaattagct atcagcacct gcatggctaa
    13321 tccttctggt tgtactgtca tggtcactca ggctgccgaa gcgggcgcgg gaatcgccac
    13381 gggtgcggta acggtaggca acgcttggga agcgcctgtg ggggcgttgt cgaaagcgaa
    13441 ggcggccaag caggctatac caacccagac agttaaagaa cttgatggct tactacaaga
    13501 atcaaaaaat ataggtgctg taaatacacg aattaatata gcgaatagta ctactcgata
    13561 tacaccaatg agacaaacgg gacaaccggt atctgctggc tttgagcatg ttcttgaggg
    13621 gcacttccat aggcctattg cgaataaccg ttcagttttt accatctccc caaatgaatt
    13681 gaaggttata cttcaaagta ataaagtagt ttcttctccc gtatcgatga ctcctgatgg
    13741 ccaatatatg cggactgtcg atgtaggaaa agttattggt actacttcta ttaaagaagg
    13801 tggacaaccc acaactacaa ttaaagtatt tacagataag tcaggaaatt tgattactac
    13861 atacccagta aaaggaaact aactaaatat gagtaacttt gaaaaaaaat atattttaga
    13921 attaaatgat gctttaagcc atttaaatca taactctacc tcatttgatt tattgaaagt
    13981 tttgatttca tggttatcaa acgatattgt cattgataaa tttaaaattt taggttatga
    14041 ctttagtaaa tatatcgaaa tgaatcccga tgactatccg gttgaaaaat ctatattgaa
    14101 tagagaggaa attatttatc tcaaaaacaa tatttatcgt aaaatatcat caggaaattt
    14161 taaatttcaa tactttgtac aatatattag agatatttta gaatatttat ttattgaaca
    14221 tattgaaaga gtctgtcctt actgcgaatg gggtgaaatg caaaaattag aagaacaaaa
    14281 tacgcatgaa acggtgtatc tctgtactca atgtggatgt gctttttata acgataattc
    14341 acaattttta ttaaaaaccc ctttaaccat tccaatgaaa cgtgatgaat ttaaataaac
    14401 aagccgtagc ctgcatgaac cctaaaatcc acgtgtagcg tgtgtgcgcc agcacgcatg
    14461 cgttccatga tttacggctc aatgccgtct gaaaagctca caatttttca gacggcattt
    14521 gttatgcaag taaatattca gattccctgt atgctgtaca gacgcgggag tgttaagccc
    14581 cccttgtttg aagctccgcg gctcctgccg agcttcaccg accccgttgt gcccaagctc
    14641 tctgctcccg gcggctacat tgtcgacatc cccaaaggca atctgaaaac cgaaatcgaa
    14701 aagctggcca aacagcccga gtatgcctat ctgaaacagc tccaagtagc gaaaaacgtc
    14761 aactggaacc aggtgcaact ggcttacgat aaatgggact ataagcagga aggcttaacc
    14821 agagccggtg cagcgattat cgcgctggct gttaccgtgg ttactgcggg cgcgggagtc
    14881 ggagccgcac taggcttaaa cggcgcagcc gcagcagcgg ccgatgccgc ctttgcctca
    14941 ctcgcttctc aggcttccgt atcgctcatc aacaataaag gcgatgtcgg caaaaccctg
    15001 aaggaactgg gcagaagccg cacggtaaaa aatctggttg tagcggcggc aacggcaggc
    15061 gtatccaaca aactcggtgc ctcttccctt gccacttgga gcgaaacccc ttgggtaaac
    15121 aacctcaacg ttaacctggc caatgcgggc agtgccgcgc tgatcaacac cgctgttaac
    15181 ggcggcagcc tgaaagacaa tctggaggca aatatcctgg cggcattggt gaataccgcg
    15241 catggggagg cggcgagtaa gatcaaagga ctggatcagc actatgtcgc ccacaaaatc
    15301 gctcatgccg tagcgggctg tgcggctgca gcggcgaata agggcaaatg tcaggacggc
    15361 gcgatcggtg cggctgtggg tgagattgtc ggggaggctt tggttaaaaa taccgatttt
    15421 agcgatatga ccccggaaca attagatctg gaagttaaga aaattaccgc ctatgccaaa
    15481 cttgcggcag gtacagttgc aggcgtaacg ggaggagatg tcaatactgc tgcacaaacc
    15541 gcacaaaacg cggtagaaaa taatgcggtt aaagctgttg taactgctgc aaaagtggtt
    15601 tataaggtag ccagaaaagg attaaaaaac gggaaaatca acgttagaga tttaaaacag
    15661 acgttgaaag acgaaggtta taatttagcc gacaacctga ccaccttatt cgacgaaaca
    15721 ttggattgga acgatgccaa agccgttatt gatattgtcg tcggaacaga gctgaatcgc
    15781 gctaataaag gggaagcggc acaaaaggtc aaggaagttt tagaaaaaaa tcgtccttat
    15841 atccctaata aaggtgctgt accgaatatg agtacataca tgaaaaataa tccttttgga
    15901 aaacagctgg ctcaaatttc agaaaagaca acgcttccga cgcagcaagg gcagtctgtc
    15961 ttcttggtaa aaagaaacca agggttatta aaaaccggtg ataggtttta tttagatggc
    16021 caacataaaa atcatttaga ggtttttgat aaaaatggga actttaagtt tgttctaaat
    16081 atggatggtt cgcttaacca aatgaaaact ggggcagcaa aaggtcgtaa attaaactta
    16141 aaataggaaa ttttatggaa acattgaatg atataaaaaa aatcttgatt aatgtggggc
    16201 tttatcaagg gtttgatttg acagatccaa aagtatcaga agaagttaat catgaaacag
    16261 ctaatatgaa atggattaaa gattatactt cagacgggaa ttgggataat gaatttaagg
    16321 aggatttaaa aaacttttta gattatatgg aagtatgcca attagcccta aacgataaaa
    16381 atttcaaaat tgccagtaat tctttattta tggctatgat ttacgcaggt aatctatctc
    16441 ttatatttga ttcaataaaa actgatatat caacattatt gagtgctgag tataaaaaga
    16501 atagtttttc atggccatct cttgatgaat agaaagcaag ttgtagcctg catgaaatct
    16561 aaaacccatg cataaggtgt gggcttcagt atacgcgttc catgatttac ggccatatgc
    16621 cgtctgaaaa gctcaatttt ttcagacggc atttgttatg aaagtaaata tttagattcc
    16681 ctgtatactg tttagactcg tgtgtgctga gtaagctgta gtctgcatga aacctaaaac
    16741 tcgctcaaaa ttaagctaag acattagcag ggcaagggcg aaaattgaat cttaaataag
    16801 gtgattcaga tgaaaaattt taatgtagta aaagaaagtt taagagagtt aggaattaaa
    16861 caaggatttg atctttatga gaaagccaca actgaaaaat tgaatagtga agatcctctt
    16921 gacttacaat ggctttctaa ctattcatct gattggaatg atgaattaga agaagacttt
    16981 gattcttttt ttcagcatat gaaggaatat caatatgcta ttgacaatga agacattaaa
    17041 tctgcatgta gttcactatg tgaagctatg ctctatgttg gtaatattaa aaattttttt
    17101 gagtttctca aaagcgatat gattagactg ttgagaggtg aaagtaaaac aacagacttt
    17161 caatggccgc aatttgatga atagcagcaa gctgtagcct gcatgaaacc taaaatccat
    17221 gcgtaaggtg tgtgcttcag cacgcacgcg ttccatgatt tacggctcaa tgccgtctga
    17281 aaagctcaca atttttcaga cggcatttgt tatgcaagta aatattcaga ttccctatat
    17341 actgcccaga tgcgtgcgtg ctgaagacac cccctacgct tgctatttga aacagctcca
    17401 agtcaccaaa gacgtcaact ggaaccaggt acaactggcg tacgacaaat gggactataa
    17461 acaggaaggc ttaaccggag ccggagcagc gattattgcg ctggctgtta ccgtggttac
    17521 tgcgggcgcg ggagccggag ccgcactggg cttaaacggc gcggccgcag cggcaaccga
    17581 tgccgcattc gcctcgctgg ccagccaggc ttccgtatcg ctcatcaaca acaaaggcaa
    17641 tatcggtaac accctgaaag agctgggcag aagcagcacg gtgaaaaatc tgatggttgc
    17701 cgtcgctacc gcaggcgtag ccgacaaaat cggtgcttcg gcactgaaca atgtcagcga
    17761 taagcagtgg atcaacaacc tgaccgtcaa cctggccaat gcgggcagtg ccgcactgat
    17821 taataccgct gtcaacggcg gcagcctgaa agacaatctg gaagcgaata tccttgcggc
    17881 tttggtgaat actgcgcatg gagaagcagc cagtaaaatc aaacagttgg atcagcacta
    17941 cattacccac aagattgccc atgccatagc gggctgtgcg gctgcggcgg cgaataaggg
    18001 caagtgtcag gatggtgcga taggtgcggc tgtgggcgag atagtcgggg aggctttgac
    18061 aaacggcaaa aatcctgaca ctttgacagc taaagaacgc gaacagattt tggcatacag
    18121 caaactggtt gccggtacgg taagcggtgt ggtcggcggc gatgtaaatg cggcggcgaa
    18181 tgcggctgag gtagcggtga aaaataatca gcttagcgac aaagagggta gagaatttga
    18241 taacgaaatg actgcatgcg ccaaacagaa taatcctcaa ctgtgcagaa aaaatactgt
    18301 aaaaaagtat caaaatgttg ctgataaaag acttgctgct tcgattgcaa tatgtacgga
    18361 tatatcccgt agtactgaat gtagaacaat cagaaaacaa catttgatcg atagtagaag
    18421 ccttcattca tcttgggaag caggtctaat tggtaaagat gatgaatggt ataaattatt
    18481 cagcaaatct tacacccaag cagatttggc tttacagtct tatcatttga atactgctgc
    18541 taaatcttgg cttcaatcgg gcaatacaaa gcctttatcc gaatggatgt ccgaccaagg
    18601 ttatacactt atttcaggag ttaatcctag attcattcca ataccaagag ggtttgtaaa
    18661 acaaaataca cctattacta atgtcaaata cccggaaggc atcagtttcg atacaaacct
    18721 aaaaagacat ctggcaaatg ctgatggttt tagtcaaaaa cagggcatta aaggagccca
    18781 taaccgcacc aattttatgg cagaactaaa ttcacgagga ggacgcgtaa aatctgaaac
    18841 ccaaactgat attgaaggca ttacccgaat taaatatgag attcctacac tagacaggac
    18901 aggtaaacct gatggtggat ttaaggaaat ttcaagtata aaaactgttt ataatcctaa
    18961 aaaattttct gatgataaaa tacttcaaat ggctcaaaat gctgcttcac aaggatattc
    19021 aaaagcctct aaaattgctc aaaatgaaag aactaaatca atatcggaaa gaaaaaatgt
    19081 cattcaattc tcagaaacct ttgacggaat caaatttaga tcatattttg atgtaaatac
    19141 aggaagaatt acaaacattc acccagaata atttaaagga aaaattatga aaaataatat
    19201 ttttctaaac ttaaataaaa aatctataaa taacaaccat tttgttattt cgattttttt
    19261 tgaaacaatt taccaatttg aaactaaaga tacgctttta gagtgtttta aaaatattac
    19321 aactaccgga cattttggag taataggtgc tcaatatgaa aaaatagatg ctaccagatg
    19381 gattggagat tatgaagagg taaatggatt tgagtatatt gataaagctc cttctattta
    19441 tttttcagtt ggagatgatt tcaatcctga agaattaatt atacctatta atttagcata
    19501 tcattacttt aatattgcaa tatctgattt cttaatagct caccctgaat atcaaaaaaa
    19561 gtgtaaagaa atacaaaaaa catattctca aacaaactgt agcctgcatg aaacctaaaa
    19621 tccatgcgta aggtgtgtgc ttcagcacgc acgcgttcca tgatttacgg ctcaatgccg
    19681 tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat tcagattccc
    19741 tatatactgc ccagacgcgt gcgtgctgaa gacaccccct acgcttgctg cagaactttc
    19801 gggtaaaacc ggtgtgagca ttagcgcacc gtatgccaat gagaacagtc gcatcctgct
    19861 cagcaccacg gatatcagtt cggaaaacgg caaaatcaaa attcaatctt acggtgacca
    19921 atattactat gcgagacaga gcgaactcta tacctttgaa cgccgcagct acaaaactgg
    19981 caaatggtac aaccgcaaac acattaccga agtcaaagaa cacaaaaacg ccaagcccga
    20041 cgcagtaacc ctcagcgcat cccaaggcat cgacatcaaa tctggtggca gcatcgacgc
    20101 ctacgccacc gcattcgatg cccccaaagg cagcattaac atcgaagccg ggcggaaatt
    20161 gacactctat gccgtagaag agctcaacta cgacaaactt gacagccaaa aaaggcgcag
    20221 atttctcggc atcagctaca gcaaagcaca cgacaccacc acccaagtca tgaaaaccgc
    20281 gctgccctca agggtagttg cagaatctgc caatctgcaa tcaggttggg ataccaaact
    20341 gcaaggcaca cagtttgaaa ccacactggg tggcgcaacc atacgcgcag gcgtaggcga
    20401 gcaggcacgg gccgatgcca agattatcct cgaagggatc aaaagcagca tccacacaga
    20461 aaccgtgagc agcagcaaat ctactctatg gcaaaaacag gcaggacggg gcagtaacat
    20521 cgaaaccttg caattgccga gtttcaccgg tcccgttgcg cccgtactgt ccgcacccgg
    20581 cggttacatt gtcgatattc cgaaaggcaa tctgaaaacc caaatcgaaa ccctcaccaa
    20641 gcagcccgag tatgcttatt tgaaacaact tcaagttgcg aaaaacatca actggaatca
    20701 ggtgcagctt gcttacgata aatgggacta caaacaggag ggcatgacac ccgcagcagc
    20761 agctgtcgtc gttatcgtcg taaccgtatt gacctacggc gcactgtccg ccccggcagc
    20821 cgccggaacg gcgggcgcgg caggcgcagg agcgggagga gccgcagcag gaacggcagc
    20881 cggaactgga gtagcagcag gaacggcagc cacaaccgga gtagcagcag gcacatcagc
    20941 tgcagctatc accacagccg caggcaaagc cgcactggcc agtctcgcca gccaagccgc
    21001 agtttccctc atcaacaaca aaggagacat aaaccatacc ctgaaagaac tgggcaaaag
    21061 cagcaccgtt agacaggccg ccaccgccgc cgtaaccgca ggcgtactgc agggcataag
    21121 cgggctgaac acccaagcag ccgaagccgt cagcaaacat tttcacagtc ccgcagcagg
    21181 caaactgacc gctaacctga tcaacagcac cgctgccgca agtgtccata ccgccatcaa
    21241 cggcggcagc ctgaaagaca acttgggcga tgccgcactg ggtgcgatag tcagtaccgt
    21301 acacggagaa gtagcgagca aaatcaaatt taatctcagc gaagactaca ttgcccacaa
    21361 gatagcccat gccgtagcag gctgtgcatc ggcggtagca aataaaggca aatgtcggga
    21421 cggcgcaatc ggcgcggcag tcggcgagat ggtgggagaa accctgttgg acggacgcga
    21481 tgtaggcaaa ctgtcacccc aagaacgcca aaaagtcata gcctactcgc agattatcgc
    21541 aggcagcgca gtggcattgg ttaaagggga tgtgaatacg gcggcgaatg cggctactgt
    21601 ggcagtggag aataatagtc ttttagctcg caggagggta aatatacgtt ggacttcgcg
    21661 acaagaattg gaacatgaat atgccattct tgaaatccag gccattacca atcaaatccg
    21721 aaggctggat ccgaaattta acgggattgc tattatgagg aatcctagag agccgtggac
    21781 aagacatgat gtacaaacat acaggcaata ttataatcaa ttaagggaat ccagaggctt
    21841 tgctgttgac ccaatttata gaatcaggat aaacaacggc aatgaattta accgtatcat
    21901 gtcatcaaaa tacccttata atgagcttta tgtagccaat cctaaatcgg cgacggggta
    21961 ttttagggta gattcgtata atcctgcgac agaggaaatt atttcaagaa aatttaccca
    22021 attttctcaa atccaagaaa gtacggggat tggttatatc aaggaggctg ttagaaaata
    22081 tagccctggt gctgtcattt ccaatgttcc aagtacacct actacgataa gaggaagaaa
    22141 gcttg
//
LOCUS       DAWISC010000013        21315 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWISC010000013 DAWISC010000000
VERSION     DAWISC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.013"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(79..1965)
                     /gene="uvrC"
                     /locus_tag="WIW87_000354"
     CDS             complement(79..1965)
                     /gene="uvrC"
                     /locus_tag="WIW87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ5173568.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVRLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2029..4191)
                     /locus_tag="WIW87_000355"
     CDS             complement(2029..4191)
                     /locus_tag="WIW87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ5173569.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4330..5280
                     /gene="trxB"
                     /locus_tag="WIW87_000356"
     CDS             4330..5280
                     /gene="trxB"
                     /locus_tag="WIW87_000356"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ5173570.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5436..5804
                     /gene="rpsF"
                     /locus_tag="WIW87_000357"
     CDS             5436..5804
                     /gene="rpsF"
                     /locus_tag="WIW87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ5173571.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5805..6107
                     /gene="priB"
                     /locus_tag="WIW87_000358"
     CDS             5805..6107
                     /gene="priB"
                     /locus_tag="WIW87_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ5173572.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6114..6344
                     /locus_tag="WIW87_000359"
     CDS             6114..6344
                     /locus_tag="WIW87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ5173573.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6361..6813
                     /locus_tag="WIW87_000360"
     CDS             6361..6813
                     /locus_tag="WIW87_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ5173574.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(7006..7812)
                     /locus_tag="WIW87_000361"
     CDS             complement(7006..7812)
                     /locus_tag="WIW87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5173575.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(7813..8559)
                     /gene="pssA"
                     /locus_tag="WIW87_000362"
     CDS             complement(7813..8559)
                     /gene="pssA"
                     /locus_tag="WIW87_000362"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ5173576.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(8633..9037)
                     /locus_tag="WIW87_000363"
     CDS             complement(8633..9037)
                     /locus_tag="WIW87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173577.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9143..9625
                     /locus_tag="WIW87_000364"
     CDS             9143..9625
                     /locus_tag="WIW87_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ5173578.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(9677..10888)
                     /locus_tag="WIW87_000365"
     CDS             complement(9677..10888)
                     /locus_tag="WIW87_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ5173579.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11111..13549
                     /locus_tag="WIW87_000366"
     CDS             11111..13549
                     /locus_tag="WIW87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ5173580.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            13757..15067
                     /locus_tag="WIW87_000367"
     CDS             13757..15067
                     /locus_tag="WIW87_000367"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ5173581.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15163..15777
                     /gene="clpP"
                     /locus_tag="WIW87_000368"
     CDS             15163..15777
                     /gene="clpP"
                     /locus_tag="WIW87_000368"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ5173582.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            15932..16168
                     /locus_tag="WIW87_000369"
     CDS             15932..16168
                     /locus_tag="WIW87_000369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173583.1"
                     /translation="MPSESQMGQQFLQGRDTRILLNLPVRLLRKDKGRKRYPAFLIYT
                     CNSSPYLPIVFSYCASIPYRFKKMPFPMGWVLYQ"
     gene            complement(16220..17485)
                     /gene="ispG"
                     /locus_tag="WIW87_000370"
                     /gene_synonym="gcpE"
     CDS             complement(16220..17485)
                     /gene="ispG"
                     /locus_tag="WIW87_000370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ5173584.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(17501..18262)
                     /gene="pilW"
                     /locus_tag="WIW87_000371"
     CDS             complement(17501..18262)
                     /gene="pilW"
                     /locus_tag="WIW87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ5173585.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18265..19359)
                     /gene="rlmN"
                     /locus_tag="WIW87_000372"
     CDS             complement(18265..19359)
                     /gene="rlmN"
                     /locus_tag="WIW87_000372"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ5173586.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19502..19927)
                     /gene="ndk"
                     /locus_tag="WIW87_000373"
     CDS             complement(19502..19927)
                     /gene="ndk"
                     /locus_tag="WIW87_000373"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ5173587.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(19986..21137)
                     /locus_tag="WIW87_000374"
     CDS             complement(19986..21137)
                     /locus_tag="WIW87_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ5173588.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5105 a         5305 c         5793 g         5112 t
ORIGIN      
        1 aatttaatcc actatatatc ggtataccga cctgctgtat catatttttt acagacggat
       61 tttgtctttg acagcatgct aatgcagatg attgtaaatc gtttccgcca atgccttgct
      121 gatgccttcc actttttcca agtcctcgcg gctggcggca atcacgccgc gcagaccgcc
      181 gaagcgggtg agcagggctt ggcggcgttt gctgcctacg ccggggatgt cgcttaagga
      241 ggaggtaacg cgggctttgt cgcgtttttt gcggtgaccg gtaatggcga aacggtgcga
      301 ttcatcgcgt acggtttgca atagatgcaa ggccgggctg ttgggcggca ggcggaagac
      361 ttcgccggta aaaggcagta tgagctcctc cataccggct ttgcgctccg ggcctttggc
      421 aatgccgacc aaagggatgt gcagcccgag ttcttcccat accgatacgg ctacgccgat
      481 ttgccctttg ccgccgtcaa tcaacacggc atccggccat ttgacggtct cgccgttggc
      541 ttcggcctcc tgcattttgc cgtaacggcg cgtcaacact tcgcgcatgg cggcgtagtc
      601 gtcgccgggt ttggcggtcg tgatgttgta gcggcggtat tgcgaaggct ggatgttttg
      661 ctcatcgtac acaacgcagg acgcaatagt ggcttcgcct tgtgtgtggc tgatgtcgaa
      721 acattcaagg cggttgaggc cgtctgaatc catgccgagg atttttgcca gttcatcaat
      781 gcggtgttgc tggctgcttt gttgcaggcg gcgttgtgca atcgccattt gcgcgttttg
      841 ttccgccatt ttcaaccgga ctttgcgttc gcctatggtc ttggtgacaa attgcatctg
      901 cttgccgtgt tcgccttcca aagcctcttt cagcgcatcg ggaacggaaa agttgctaat
      961 aatgatgtcg ggtttgcttt tgcccaaata atgttgggcg acgaaggctt cggcgtaatc
     1021 ttgtccgttt ggctcgggat cgtttttggt gtcggggaaa aagcttttat cgcctacatg
     1081 ccgtccgccg cgtatgctga cccagtgtac gcagacgagg ccgtctgaaa cggcgagcgc
     1141 gagcaggtcg atgtcgttgg ggttgttcgg gtttttgctg tcgatgaact ggttgctctg
     1201 cataatgccg agtgcttgga tttggtcgcg gtaacgtgcg gcttcttcga attgtagatt
     1261 agcggcggcg gtttgcattt tgtgttgcag ggtacgcgtc aattcgtcag ttttgccatt
     1321 aaggaaagtc gcggcttcac gcacgctgtc acgataatct tcttcactga tgtggcctac
     1381 acaaggcgcg gtgcagcgtt tgatttggta aagcagacaa ggacggtcgc gatgctcgaa
     1441 tacactgtct tcgcaggtac gcagcataaa gactttttgc aacacttgaa tgctgtcgcg
     1501 cacggcgttg ctgttgggat atgggccgaa atattgatta ggctttttca gcgtgccgcg
     1561 gtaatacgcc atttgcggat attgatggcc gctgagcatc aaataaggat agcttttgtc
     1621 atcgcggaaa agaatattgt atttgggcga cagggctttg atgaagttgt tttcgagaat
     1681 cagggcttcg gattcggagc gggtgatggt ggtttcgatg tggtgaacct gtttcaccat
     1741 caatgcgatg cgcggggaat ggtcgttttt ctggaaatag ccggacacgc gccgcttgag
     1801 gttgacggct ttgccgacgt ataagacgtt gccgctttcg ttgaaaaaac ggtatacgcc
     1861 tggcaggttg ggcaggtttt tgaggaaaag tgggatgtcg aagtgttccg gaaaacgggt
     1921 ttctttattc atatcaatta cttgaaaaac ggtttcagac ggcatattac catatcgctg
     1981 gagacagcct tatccgttcg gacaaggctg cagggcggtt acatgctgct aatcgatttt
     2041 tacccgtttc aggcgcaagg cattgctcaa cacggaaacc gagcttgccg ccattgccgc
     2101 gccagcgatg acgggattta aaaagccaag cgcggcgaga ggaatgccca aaatattgta
     2161 gaagaaggcg aaaaacaggt tttgcttgat gtttttcaaa gtggcttgcg acaccagcag
     2221 agcatcggcg agttggttga ccgaatgctg catcagcgtg gcggatgcgg tatgttcggc
     2281 aacgtccgct ccgcctttca tggcgaagct gacgttagcg gcggcaagcg cgggcgcgtc
     2341 gttgatgccg tcgccgacca tcgccacggt tttgccggcg gctttgagtt tttgcacttc
     2401 ggcagcttta tcgcgcggac tcatgttgcc gaaggcgtgt gcgatgccca gttgtttggc
     2461 gacgtattcg accgtgcctt ggttgtcgcc gctcataata tagacatcga tattgtgttt
     2521 tttcagacgg cctatggctt cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc
     2581 gccgatgggt ttgttatcga ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc
     2641 taaaaacttc ggcaaggcca gttcggcaaa ttcggctttg cctgctttca ccaaacccac
     2701 gccttccact tcggcggtaa tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg
     2761 aatgtccaaa ccgcgcgctt gggcggcgga gacgatggca cgggcgagcg gatgggcggc
     2821 gttttgttcg acggcggcgg cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg
     2881 aacgcaataa acggcggcaa cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa
     2941 cacgacggca tcgacgtggg cggcttcctc cattgctgcc gcgtctttaa accaaatacc
     3001 gtgtttaacc gctttgccca taccgaccat aatcgcggca ggggttgcca gacccagcgc
     3061 gcacgggcag gcaatcacca aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc
     3121 cttaatcagc caagtaacaa taaaagtcaa caacgcaatg cccacgacgg caggcacgaa
     3181 taccgcagcc gctttatcgg ctacgcgcgc aatcggtgct ttactgcctt gtgcttcaga
     3241 gagcgcgttc atcatgtcgc cgagctgggt ttggctgccg agctgcgtgg cgcggtacac
     3301 cacactgcct tcggtcatta acgcgcccgc caacactttg ccgcccgcct ttttttcttc
     3361 aggattggat tcgccggtaa gatggctctc gtccgcccaa ccgctgccgc tttcaatgat
     3421 gccgtctgcg gcaatgcgtt cgccgtggtt ggcgcggata aggtcgccga tttgcacttg
     3481 gtcgatggga agctgtttcc attcgccgtt gcgttgcacg ttgacttggg ttggtgtaag
     3541 tttgagcagc aagcccaagc tgttgaggct ggattttttg gtacggtgtt ccaaaaattt
     3601 acccagtgac acaaaaccga tcaccatcac gcccacttca aaatacacat gcgccatacc
     3661 gtacgccgcg tgcgggctga aaaacagcat atagacggaa tacaggtaaa tcgagaccgt
     3721 gccgatggta accagcacgt ccatattcgc cagtccgccc ttaatgctcg cccacgcgct
     3781 tttgtaaaac gggattgcca gccaaagctg caccacgctt gccaatgcga actgccacaa
     3841 cggcggaatc atccaatcgt gtctgccgat catcatcccc gccatgccga taaggaacgg
     3901 gacgttgatg gtgaacagca gccacagccg ccagccgata tggtgttctg cttcgggttg
     3961 cggcaatgta tcttccgttt tttccttcgc gccgtaaccg gttttctcaa tgattttggc
     4021 aatgtcggct actgaggttt tgctgtcgtc aaacactacc tgcgcctctt cgctggcgaa
     4081 gtttaccccc gccgattcga caaaatcttt tttgttcaac actttttcaa tgcgcgaagc
     4141 gcaggcctgg caggtcatgc cttcgatttg gaaacggatt ttttgttgca taagcgtgat
     4201 tcctgctttg atattcagac ggcagttaag gctgcgggat gccgtctgag cgccggtatg
     4261 gtcgaaattt ctcaaacgcg ccgataattc gagtagaatc cgaaaacttg acacagcaaa
     4321 ggaaaaacaa tgtcccaaca ccgcaaactg attattttgg gttccggccc cgccggatac
     4381 accgccgccg tctatgccgc gcgcgccaat ttaaaccccg tcattattac aggtatcgcg
     4441 caaggcgggc aactgatgac caccaccgaa gtggacaact ggcctgccga tgccgacggc
     4501 gtgcaagggc cggaattgat ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa
     4561 atcatttttg accaaatcaa cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc
     4621 gatatgggcg agtacacttg cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac
     4681 ctcggtttgc cgagtgagga agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc
     4741 gacggtttct tctataaaaa ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt
     4801 gaagaggcac tctaccttgc caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc
     4861 gagttccgtg ccgaaaaaat catgatcgac aaactgatga aacgcgtgga agagggcaaa
     4921 atcatcctca agctggaaag caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac
     4981 ggcgcattat taaaaaacaa cgacggttct gagcaacaaa ttgccgtcag cggcattttt
     5041 atcgccatcg ggcacaagcc gaataccgat attttcaaag gtcagttgga aatggacgaa
     5101 gccggttacc tgaaaaccaa aggcggcacg gcggacaatg tcggcgcaac caatatcgaa
     5161 ggcgtatggg cggcgggcga cgtaaaagac catacctacc gtcaggcgat taccagcgcg
     5221 gcttccggct gtcaggcggc tttggacgcg gaacgctggc tgggcagcca aaacatttga
     5281 atgccgtctg aaaccgaaat tttcggcgga atgcaaattt ttcgcaccaa atccttgatt
     5341 ccgtcggcaa accccaatat aatgccgctt ctttaaacct tgccttctgc tttcgcatgg
     5401 cagaaggctg tttttaccat ccataaggag ataacatgcg tcattacgag atcgtgttta
     5461 tcgttcatcc cgatcaaagc gagcaagtac ccgctatggt tgaacgttac aaaaccatga
     5521 ttgccgaagc aaacggtaag attcaccgtc tggaagactg gggtcgccgc cagctggctt
     5581 acccgattaa caaaatccat aaagcccatt acgttttgat gaacatcgaa accactcccg
     5641 aagtggttga agagctggaa accgcattcc gcttcaatga tgcagtattg cgtcatctga
     5701 ccatcaaaac caaacacgcc gttaccgaag catcccctat gttgggtggt gaaaaggcta
     5761 agaacctgtt gagcggtgcg tctgaagaag cggtcgccca ataattggga ttcaataatc
     5821 ttgtttcgct tgccgcgtta attgaaaagg ttttccctat tcgatatacg cctgccggaa
     5881 tccctgtttt agatattatt ttaaagcacg aatcgtggca ggaggaaaac gggcagcaat
     5941 gccttgtcca attggaaatt ccggcacgga ttttaggcag gcaggcggaa gagtggcagt
     6001 atcggcaagg tgtatatgtt cacgtcgaag gttttttagc tcaaaaaagc agacgttccc
     6061 ttatgccgat gctcaggata caaaatattc aagaatataa aggttaaacg acaatggctc
     6121 gtcaatcatt caaacgtaga aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg
     6181 attacaaaca agttgatttg ctgaaagact tcatctccga aaacggtaaa atcattcctg
     6241 cacgcatcac aggaacgaag gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg
     6301 cacgcttcct ggctctgctg ccttacaccg accaacacaa ataattttgg agattgaatc
     6361 atgcaaatta ttctgttaga aaaaatcggc ggtctgggca acttgggcga catcgtaacc
     6421 gttaaaaacg gctacgcccg caactttcta attcccgcag gtaaggcaaa acgtgcgacc
     6481 gaagcgaata tgaaagagtt tgaagcacgc cgcgcagaac tggaagccaa acaggctgaa
     6541 attttggcag atgcccgagt ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct
     6601 caaaaagctg gtgtggacgg tcgcctgttc ggttccgtta ccaatgccga cattgctgct
     6661 gcaatcgttg ctgccggcat cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct
     6721 ctgaaagccg ttggcgagta cgaagtggaa gtggctttgc acactgatgc cgttgctaaa
     6781 attaccgttg ccgtcgttgc cgcaaccgag taattgaaat tatcagatgc cgtctgaaac
     6841 cctgccgatt gtcggaatgt ggtttcagac ggtatttgtt ttgtttttcg tatattctgc
     6901 aaaggtctcg gtcaggttgt ctgtcctttt gtcggaacag gagactgggc agctttgtcc
     6961 tgattaattg cttgtgcaaa cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta
     7021 cagcattttt ccggcaatca aaagcaacat aatgccgaag gattttttga gtttggcaga
     7081 agaaagtttg tgggcggttt tgacaccgag cggggcaaag gcaatggttg ccgcgctgag
     7141 gacggcgacg gcgggcaggt aaaggaagcc cagtgaccct tcgggcaatc ctgcaatatt
     7201 caggccgttg agcagatacg atattgcgcc ggagagtgca atcggccagg caaggccgga
     7261 tgatgtgccg atggctttat gggcggggaa gccgcagtgg attaagaagg ggacggaaag
     7321 tgaaccgccg cctatgccga cccagctcga cattgtgccg aacagtgtgg aaaccgcagt
     7381 cagtccgggc agtccgggca gcgggcggga tgccgtctga gggtcggtat gcagtgtttt
     7441 gaatgcgacg gcggttaaaa acaggatgaa gaaaatttga agcccgaacg cggggatata
     7501 ttttgcggag agtgcgcccg tgaatacgcc gaatatcata cccggcatca tcgtaaatac
     7561 ggttttccag tcgaccgcct gttttttgtg ctgccccagc atactggaaa aggcggtgaa
     7621 gaccatgacg gcgaaggatg tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa
     7681 accctgcaaa tcaagcaccc ataaaacgac agggacaatc agcgtgccgc cgcctacgcc
     7741 gaacaggccg gcaataaaac ctgccgcact gcctacggca agcaggatta agataatgtc
     7801 ccaatgccac atttaatccg cctttctgta ctttttccaa aatcggcgtg ccgccataat
     7861 gtagccggac aggctgtatc cgagaaagaa caggaagagg acgagcgacg gttcccaagt
     7921 gaccagaagc agcagtaaga cggcaagcag cattccgaca aaggggactt gtctgcggat
     7981 gttgatttct ttaaaactcc aaaaagggat ttggacaatc atcgacaggc cggcaaacag
     8041 tgtgatgccc aatgcccacc agtggacggc ggggaatttt tcgacgctgt ggttgaccca
     8101 aatcagcccg acaatcagcg cggcggcagt cggactgggc acgccgataa accagcgttt
     8161 gtccaccttg ccgatgagtg tgttgaacag ggcgaggcgc agggcggcgc aggcgcagta
     8221 gatgaaggcg acggaataac cgattttgcc gaactgccaa agctgccatt tgtaggcaat
     8281 cagagcggga gcaacgccga agctgaccat atcggcaagg ctgtcgagct gctccccgaa
     8341 cgcgctttgg ctgttggtca gccgcgccac gcgcccgtcc ataccgtcca gcaacataga
     8401 gatgaatacc gcgatggcgg cggtttcata acgtccgtgc atggattggg tgattgcgta
     8461 aaacgcggaa aacagcgcgg cgatagtaaa ggaattgggc agcaggtaga tactgttttg
     8521 acgcagggag cgttgcgggg tgttggtcgg ggtttccata gctgaagtgt ctcacggacg
     8581 tggagaatcg ataggggatt ataacccaaa ccattatcga acaatacgcc ctttaggatg
     8641 aagccctcgg aggcggtttg tttttctgat ggcggatgct ttgcggggcg gtttgactct
     8701 gcctgccgcc gggtgtgttg tttcggattt ttgccggatg gatgttgttg ccgggcattt
     8761 ctgggcttgc cggaaggtat cggggggagg gcatcggaat atgcggcgcg ccgttcaaac
     8821 cggggcggcg tttccctatt ttcggggagt ttgcaggctt caacaaattg aaagatattt
     8881 tttgcatttc cgaccgtatg ctgattttga aacgggataa attataggac aaggtttttc
     8941 gcccgccgca aaggttggcc gcaatgccgt ctgaaaacaa cggcttgccg ctaaggcggc
     9001 ggtatgcgga tttgcgctgc tgaaatcggt ttgccatacc cggcggtgca atctgcgggg
     9061 agggataagc cgataaggcg ggacttatcc tgttttttgt ttataatgca acagaatttg
     9121 gacgaaccgt cgggaattta tcatgatgaa aaagatactg gccgtatcgg cactatgcct
     9181 gatgactgcg gcggcacggg ctgccgatac ttacggctat ctcgccgttt ggcagaatcc
     9241 gcagaatgcg gacgatgttt tgcaggttaa aaccacaaaa gaagattcga cgaaaagcga
     9301 agcgttttcc gagttggaag ccttctgcaa aggtcaggac acgcttgcgg gcattgctga
     9361 agacgagccg accggatgcc ggtcggtcgt gtcgctgaac aatacctgtg tcgcgctggc
     9421 atacccgaaa gccttgggcg cgctgcgtgt cgacaacgcc gtcgtgatta cttctccgcg
     9481 ttttacgagc gttcatcagg tcgcactcaa ccagtgcatc aaaaaatacg gcgtacaggg
     9541 acaatgcggc ttggaaacag tgtattgcac atcttcttct tattacggcg gaactgtgcg
     9601 ctctttgatt caaaatctca aataaaacgg aaaatgccgt ctgaaagatg ttcagacggc
     9661 atttctatat cgacggtcag gattctttcg gatcgggcag caggctgttc aacataatgg
     9721 caagtacggc gcacaagccc acgccggcaa agctgaagct gcccaatttg agcgtcatgc
     9781 cgccgatgcc cgtggtcagt accgagctga cgatgaccag gtttttcggc agcatcaaat
     9841 cgactttggc atcaatcagc gttttcacgc ccaaagaagc aatcgtgccg aacagcagca
     9901 gcataatgcc gcccattact ggcatcggaa tggaagccaa aaacgcattg aatttgccga
     9961 aaaacgccat gcagacggca aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt
    10021 tggtaatcat caccgcaccc gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca
    10081 gacccgcaac gcatacgccc aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt
    10141 tcgtatagtc tttccccgtc acattgccga ttgccatgat gccgccgatg tgttcgatgg
    10201 cgggggcgac ggcaacgggc agcataaaca gtgcagcctg ccagttgatc tgaggcgttt
    10261 caaaatgggg aacggcgaac cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca
    10321 tcagcagtgc caaaacataa cccgaagcga caccgatcaa gatgggaatc agcttcatca
    10381 tcctgctgcc gaaaaccgat acgatggcgg taacggcaaa ggtaaagccg gaaagaatca
    10441 gcgaatcggt atagtcgatg acctgtttgc cgtccgcctg acccattgcc atgctgcttg
    10501 ccgccacggc aacagacaga ccgatgacca tgatgacggg gccgatgacg accggaggca
    10561 gcagtttgtg taccgctgcc agtccgcgcc aacggatcag cgcggcaaac acaaaataca
    10621 taaagccggc ggcaaacagt ccgaacatgg tggaaggcag cccccattcg ccgacggagt
    10681 agataatcgg tgcgataaag gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg
    10741 ttgtgatttg gaacagcagc gttcccaagc ctgcgcccaa aagcgcaaga gccggattca
    10801 gaccggtcag caggggaacc agcaccattg cgccgaatgc cacaaataaa atctgtgcac
    10861 cggaaacggc aagtttcagt tggttcatac catcttcttt caatcggttc aaacggcacg
    10921 gattatactg gtttgccgag ggtctgcgta aaaatacata cgtcgggcaa cagatatgcc
    10981 gtctgaaagg caatgtctgt ctgttgcagg atgaaagcag gagaaatgga gtataatccg
    11041 acactttcca tattcaatcc gcaagccgtc cggcttcctt atttcagacg gcattttccg
    11101 gtaatacggc atgacagaaa aatcccataa aaaaacagca aaaggcaggg cgggaagccc
    11161 gtccccaacg tccgcacgca acaagaaagc cgacaacggc gcacggggca ataaagtttc
    11221 cgagcggctc aaggcggtca aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga
    11281 acatgtcgtc aaccttatcg gcgacgcact gtggctgatg ggtttggcgg caaccctgta
    11341 tttggcgatt tccctgatca gtttcgatat gggcgatccg tcttggtcgc acagttcgcc
    11401 ggttgtggaa gatgtcgcca attggggcgg actgttcggc gcgtatgttg ccgatgtcgg
    11461 ctattatctt ttcggctggt cgttctggtg gtggatagcg gctgcctgcg tcgtgctgta
    11521 taaaaatttc cgcctgcacg caaaacagac ggaaaacgag gcatacaacc acaaaatcgc
    11581 tgccgccgcg ctgtttgtcc tgacggtctt cagccccgtc ttggagtatt ttgtgctggg
    11641 cggaaaatat gccgactccc tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg
    11701 cgcagtgttt gcgtggctgc tggggaaatc gggcagcctg ctgattatct tggttgttct
    11761 gctgttgtcg ttgtccctgc tggtgcagat ttcatggctg gaatttttga acggtgcggg
    11821 cagggcggtt caaaaccgcc tgagtgcctt atccggcaag gtcatggctt taggaaaacg
    11881 ccggccgaat accaaaacag acggtgtcga tacccaaaat acacggcgca tggtaaaaga
    11941 agccaagaat attacggcca aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa
    12001 atccgtcgcg gtttccgtcg cgccgccgcc caaaattcag gtttctctgt ttgaagatga
    12061 cgaacctcgg caggcgggcg aataccacaa gcctacattg aacctattgc ggattcctga
    12121 cagcgaaccc gtcagcatca atcccgccga attggagcgc actgccgaac tgatcgaatc
    12181 caaactggca gaattcggca tcggcgtaca agtcgtatcc gccacatccg gccccgtcat
    12241 cacgcgctac gaaatcgaac ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc
    12301 gaaagatttg gcacgctcta tgtcgctgca gtccgtgcgt atcgtcgaaa ccatcgcagg
    12361 taaaaacacg atgggcatcg agttgcccaa cgacaaacgc caagacgtga tgttgagtga
    12421 aatcttgtcc tcgcccgtgt ttgccgaagc caaatccaag ctgaccgtcg cgctgggcaa
    12481 agacattgcc ggtacccccg ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc
    12541 cggtatgact ggttcgggca agtccgtcgg cgtgaacggc atgattatgt ctatgctttt
    12601 caaagctacg cccgacgaag tccgcttcat tatgatagac ccgaaaatgc tcgagttgag
    12661 catttacgac ggtattccgc acctgctctg tcccgtcgtg accgatatgc gcgaagcagg
    12721 gcaggcgttg aactggtgcg tcgccgaaat ggaaaaacgc taccgcctgc tttcccatgc
    12781 cggtgtgcgt aatttggagg gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa
    12841 gccgctgctc aatccgttca gcctgaaccc cgacgagccc gagccgctgg aaaaactgcc
    12901 gttgattgtg gtcgttatcg acgaacttgc cgacctgatg atgaccgaac gcaaagccgt
    12961 cgagcagcaa atcgcccgtc tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt
    13021 cgccacccaa cgtcccagtg tcgatgtcgt taccggcctg attaaagcca acatcccgac
    13081 gcgtatggcg tttaccgtgc aaagcaaaat cgacagccgt accatcctcg accaaatggg
    13141 cgcggacgaa ctgctcaaat atggcgattc gctgttcctc cagcccggca gtgccgaacc
    13201 gactcgcctg caaggcgcgt ttgtttcaga cgacgaagta catcaagtcg tcaactatgt
    13261 caaatcgcaa gccccagccg actatattga aggtctgctc agcggcgagg ccgcgctgga
    13321 aactgccaat atcgttaatc cgaatgcaga cagcgacgaa ttgttcgatc aggcagtcgc
    13381 ctatgttttg gaaagcaaaa aaacctccat ttcgtctttg cagcggcagc tgcgcatcgg
    13441 ctataaccgc gcggcaaacc tgatggaggc actggaaaat gcgggtgtcg tttcttccac
    13501 cgacctcaac ggcagccgta aaattttggc gcacaaggac catttgtagc ccgtattgca
    13561 aaatgccgtc tgaacggcgg aattggcgtt tcagacggca tattatgttt caggcgaaac
    13621 attgtgatat acttgccagc taaaatttcc cctttgcggc aatgcggttc aaatatcgta
    13681 ccgttgcgct gtttgcttcc ccatgtaggg aagaaagttt atcattttat caacacaaca
    13741 aatttaaggg cttatgatga gcgtaactgt tgaaacttta gaaaatctgg aacgcaaagt
    13801 agtgttgtcc ctgccttggt ccgaaatcaa cgcagaaacc gataaaaaac tgaaacaaac
    13861 ccaacgccgt gcaaaaatcg acggtttccg tccgggtaaa gcacctttaa aaatgattgc
    13921 ccaaatgtac ggtgcgagcg cacaaaacga cgtgatcaac gagctggtgc aacgccgctt
    13981 ctacgatgtt gccgttgccc aagagttgaa agtggcaggc ttcccccgtt ttgaaggcgt
    14041 tgaagaacaa gacgataaag agtctttcaa agttgccgcc atttttgaag tgttccccga
    14101 agtcgttatc ggcgatttgt ctgcacaaga agttgaaaaa gtaaccgctt ccgtcggtga
    14161 tgccgaagtg gaccaaaccg tagaaatcct gcgcaaacaa cgcacccgct tcaaccatgt
    14221 cgaacgcgaa gcccgaaacg gcgaccgcgt catcattgac tttgaaggca aaatcgacgg
    14281 cgaacctttt gccggcggcg catccaaaaa ctacgccttc gtattgggcg caagtcaaat
    14341 gctgcctgaa tttgaagccg gcgtagtcgg catgaaggct ggcgaaagta aagacgttac
    14401 cgtcaatttc cctgaagact accacggtaa agacgttgcc ggtaaaactg ccgtgtttac
    14461 cattacgctg aacaacgttt ccgaagcgac tctgcctgaa gtcgatgcag attttgcaaa
    14521 agccttgggt attgcggatg gcgacgttgc caaaatgcgc gaagaagtgc agaaaaacgt
    14581 aagccgcgaa gtggaacgcc gcgtaaacga acaaaccaaa gaatccgtaa tgaacgcgct
    14641 gctcaaagcc gtagagctga aagcacctgt tgctttggtc aatgaagaag ccgcacgctt
    14701 ggcaaacgaa atgaaacaaa attttgttaa ccaaggtatg gctgatgctg ccaacttgga
    14761 tctgcctttg gatatgttca aagaacaagc cgaacgccgc gtatctttag gtctgatttt
    14821 agccaaactg gttgacgaaa acaaactgga accgactgaa gagcaaatca aagccgttgt
    14881 tgccaacttt gcagaaagct acgaagatcc tcaagaagtg attgactggt actacgcaga
    14941 tccttcccgc ctgcaagccc cgacttcttt ggcggtagaa agcaacgtcg ttgatttcgt
    15001 tttgggcaaa gccaaagtaa atgaaaaagc tttgtctttt gacgaagtga tgggcgcgca
    15061 agcctgatta tcctgaaaat gccgtctgaa ggcagttttt gaaagcaccg aagccgcttt
    15121 taaagctcgc tttggtgctt ttccatcatg aaaggagacg aaatgtcttt tgataactat
    15181 cttgtcccta ccgttatcga gcagagcggt cgcggtgagc gtgcattcga tatctattcc
    15241 cggcttttga aagagcgcat cgtattcttg gtcggaccgg taaccgacga gtccgccaat
    15301 ctggtggttg cccaactgtt gtttttggaa agtgagaatc cggataagga tattttcttc
    15361 tatattaact cgccgggcgg ttcggtaacg gccggtatgt cgatttacga caccatgaat
    15421 ttcatcaagc ccgatgtatc gactttgtgc ttggggcagg cggcaagtat gggcgcgttc
    15481 ttattgtcgg caggcgagaa aggcaaacgt tttgccctac ccaacagccg gattatgatt
    15541 caccagcctt taatcagcgg cggtctgggc ggtcaggcat ccgacattga aattcacgca
    15601 cgcgaacttt taaaaatcaa agaaaaactc aaccgcctga tggcgaaaca ttgcgaccgc
    15661 gatttggcag atttggaacg cgacaccgac cgtgataatt tcatgtctgc cgaagaagcc
    15721 aaagaatatg gtttgattga ccagattttg gaaaaccgcg cttctttgcg gctttaataa
    15781 aagaaacctg agagaaaccg atgccgtctg aaacgttcag acggcatttg tttcaagctg
    15841 cgtttgatat ggtatcgata ccgtcatcca cgggacggaa gaaggatttg gagtttttta
    15901 aaggatcccc gttcccgcct gtgatggacg gatgccgtct gaaagccaaa tggggcagca
    15961 gtttttgcaa gggcgcgaca ctcgcatcct cctgaatctg ccggtacggt tattgagaaa
    16021 agacaagggc aggaagcggt atcccgcctt ccttatttat acctgcaatt catctccgta
    16081 tttgccgatt gtattttcat attgcgcctc cattccttat cggtttaaaa aaatgccttt
    16141 tccaatggga tgggttttat atcaatgaaa ataaacggtt tttatttaaa aatagccaag
    16201 gtaggctggc ggttggattt tatagggagt gtatcgggat aaccttccct tgatggcgtt
    16261 tgagtccgtt ctccccataa ttggttttga catactcttc aacaatagcc agaaattccg
    16321 ttgcaatgtt gtcgcctttc agcgttactt tacgttcacc atctacataa acaggggcaa
    16381 cgggtgtttc tcccgtaccg ggcaggctga tgccgatgtc ggccaatttg ctttctccgg
    16441 gcccattgac aacgcagccc attacggcaa cgttcaggga ttcaacccca ggataaaggg
    16501 tacgccatat agacattttt tggcgcaggt aattttgaac atcttgtgcc agctcttgaa
    16561 atacggtact ggtggtacgc ccgcagccgg ggcaggcggt aaccatcggc gtaaacgaac
    16621 gcaatcccat agtctgtaaa atctcttgcc cgacgacgac ctcctgagta cgcgggctgc
    16681 caggttccgg agtcagtgaa atgcggatgg tgtcgccgat tccttcttga agcaagacgg
    16741 ataatgccgc cgttgatgcg acaatgcctt tgctgcccat accggcttcg gtcaaaccca
    16801 aatgcagcgg ataggcgcaa cggctgccca gttcgcggta aacctgaatc aaatcctgaa
    16861 ccgcgctgac tttgcacgac aggatgattt tgtcttcggg cagtcccaat agaacggctt
    16921 tttcggcaga ttccaaagcg gagacaatca gtgcttcctt cgtcacttct tcgggcggtt
    16981 tcggcgcgga agaagcgagg ttggcatcca tcatacgttt ggcgaggctt tgatccaaag
    17041 aaccccagtt tacgccgatg cggacggctt tatcgttttc agcagcagtc cgaatcataa
    17101 aggcaaattt ttcatcgcct tttacgcctt tgccgacatt gccgggattg atgcggtatt
    17161 tggacaatgc tttgccgcat tctggaaatt ccgccaacag gcgttcgccg ttgaagtgga
    17221 aatcgccgat aagcggtgtg gcatagccca tatcgtccaa gcggcggcgg atttcggcaa
    17281 ctttggacgc ggcttcgggg ctgttgacgg taatacgcac catttcggat ccggcatcgc
    17341 tcaattcctt aatctgcaat gcggtggctt ttgcatcggc agtgtcggtg ttggtcatag
    17401 attggataac gacgggtgct tctgaaccga cggtaatatg atcgatgcgg acttgatgcg
    17461 tcttgcggcg ttggagtgtg ttcatatggt ttgaatctgt ttattgaccg gtgaggacgg
    17521 tttgcaattc ttccgagtag gggaaattcg cctgcaattg tgcttcatat tcgtatgccg
    17581 cctgtgcgtt gccgagggct ttggcaattt tccagcctag cagcaaatca tcggcctgaa
    17641 ggacttctac cctgctttgg tattttttaa agtagtaatc ggcatcgccc aactgcccgg
    17701 ccagcatttt ggtgcgcgcc agttctttaa atgcgggtgg gaactgcggc tgggcggcga
    17761 gggaacgttt caaataggct tccgccaatc cgaattgccc ctgttttgcg ctgcatatgc
    17821 ctttattcag gttggcaata taaggggtcg ggtaggtggg gtcggccaga gctttgtcga
    17881 aatatgccat agattcggca gggcggttga gcctgccgca taggaaccaa ccgtagttgt
    17941 tgttgatttc ggcactgtcg ggtttgatgg agagggcttg ccggaaactt tcctgcgcct
    18001 tgtcgttaac tttcaggtat tgatagattt cggcacggac cagccaggca agctcgtttt
    18061 tagggtccga tttcagggcg tcttcaatac ttgccgtcgc ctgacggtag tcctgaccgc
    18121 gcatatattc cattgccaac tgggttttga tattggaaac ctgattggct ttttctgccc
    18181 gcgaggggcg gtaggaagtg ctgcacgcgc ccaaggcaag aacgagtaat aaagagattc
    18241 gtttggatgg cttaaaaggc ataattaccc ctgttgtccg attaaaatct gctgccattt
    18301 ttgttggcgg cgcgttttat cctgaacctg ccccgccaac tgtccgcagg cggcatcgat
    18361 gtcgtcgccg cgcgtttttc gtacggtaac gacaaatcct gcctgctgca aaatatcgcg
    18421 gaacacacgg atgttctcat tgctggagcg ttcgtatccg gagtttggga agggattgaa
    18481 cggaatcaga ttgaacttgc agggaacatc tgtgaccagt tcgatcagtt cgcgcgcatg
    18541 ttgcgcctta tcgtttattc cgtccaacat gacgtattcg aaagtgatga aatccctggg
    18601 tgctttgacc agatagcgtt ggcatgcggc catcaattct ttcaagggat attttttgtt
    18661 caacggtacg atttggttgc ggacttcgtc attggaagcg tggagggaaa ccgccaaagc
    18721 caccggcatg acatcgcgca acctgtccat ttggggaacc atacccgaag tggaaacggt
    18781 tacgcggcgg cggctcaaac cgtagccgtg gtcgtccagc atgatgctta aggcggtaac
    18841 gacattgtcg aagttcgcca tcggctcgcc catgcccatc atgacgacgt tggaaatcac
    18901 gcgctcgttt ttcggtgtaa cgcccatcgc tttgtttgcc caccacaatt gcccgatgat
    18961 ttcggcagca gtcaaattgc ggttgaagcc ctgccggccg gtcgaacaaa atgtacattc
    19021 caaagcgcag ccgacttgtg aggaaatgca gagcgtgccg cgatccgatt cggggatgaa
    19081 gacggtttcc acgccgttgc ccgtaccgac atccaaaagc cattttcgag tgccgtctga
    19141 agatttttga gacatcatca gcttgggaat ttcgatgcct gcctgttcgt tcagtttatg
    19201 gcgcaacgat tttgccaaat cggtcatttc gtcaaaattt tgcgcgccgg attggtgcat
    19261 ccaacgcata acctgtttgg cacggaaagg tttttcgccc atatcggcaa aatgtcgggt
    19321 cagcccttga aggtcgtagt tgagcagatt ggttttcatg tgtttgtgtt tttcttaaat
    19381 caaggcttca aataaaaaat tgcagggcag gcaagtttga tttgaagcct tttgcaacag
    19441 agttttcaga cggcatggat atatttatgc cgtctgaaga ggagttgggc ggtgtattgt
    19501 atcaacgggg gcagatttcg gtttggctga aaaagtaggc aatttccaaa gcggcatttt
    19561 ccacgctgtc ggaaccgtgt acggcattaa tgctgaccga agtggcaaag tccgcgcgta
    19621 tcgtgccttc ggcggcttcg gaaggattag ttgcacccat cagttcgcgg tttttcagga
    19681 cggcgttttc accctctaat acctgaatca taaccggacc gccggtcata aattcaacca
    19741 atccggcgta gaaggggcgg tctttatgaa ccgcataaaa ttcttgcgcc tctttgagag
    19801 taagctgctt cattttggcg gcaacgattt tcagaccgtt ctcctcaaag cggctgtata
    19861 ttttgccgat aacatttttg ccgacggcat cgggtttgat gatggagatg gtacgttcaa
    19921 tcgccatgct atatccttat ttttacttaa gaagaatcaa atcggtattc tatcaaaaaa
    19981 ataacttatc cgcctgaagc cttgggagat agggtcaggt gcggctgttc caaataaact
    20041 tcggactgca tttcgaccat ccggctggcg gtgcgggtaa attcttcggc aaaatcgcct
    20101 tccgtataga tatggttgac atctaccgcg ccggtggcac acagtttgac ccggaaatcg
    20161 tagagtacgt caatcagcca agtcagccgc cgcgcctccg ccttttcttg cggtgagagt
    20221 tgttccaaac ctgaaataaa aaccatttca taatgttcgg ccaaatacag atagtcggac
    20281 tgtgagcggg ggccgaagca cagtgcgcgg aaatcaaacc atatggcacg gccggactcg
    20341 gctttgtggg gaatctcccg accgtggatg gtgctgatgc cggggttcaa atcggtaatg
    20401 cctgtcattt ctttgaacag ttttgccagt tttgcctcat tttcttcatt ggcaggcgta
    20461 aagaaaatct cggcggggcg gagggtacgc agtcggtagt cttcaccgcc gtcaacgttt
    20521 aagacggtca ggctggactc gatgagcgcg attgtgggaa gaaaactgct ccggttttga
    20581 ccttgcgggt agagttcgga aggcgcgtag tttgaagtcg ccaccaaaac aacgccctcg
    20641 ttaagcaggt tttccagcag acggcctaaa atcattgcat ccgcaatatc gctgacatga
    20701 aattcgtcaa aacacaatac gcgggtttct ttggcaatct cggcggcaac ggatttcaac
    20761 gggttgcttt cgcttttcag ggttttcagc cgctggtgga tttctgccat aaaggcatga
    20821 aagtggacgc ggcgtttgcg gcggtacggg aggcagccga aaaaagcgtc catcagaaag
    20881 cttttgccgc gtccgacccc gccatagaaa taaagccctt tggggacttg cggggaacgc
    20941 aaactcctgc ctaaaaaacg gtttcttttg cgtttgaaca tcatcaattc ggtccaaagc
    21001 cgatcgaggt gttcgatggc ggctgcctgc gcgtcgtcgc ggatgaagtt gggcagttgt
    21061 gaggcagcct gataccaggt cagcgggctg tggttttcaa acggcggggc tttaaaaagt
    21121 tggtctctat tactcatatt taaccttgtt atacttcttg cggctaagaa agaaaatgct
    21181 taccgtcggt attataaatg atttccttca aacttggcat tttccgtgga aaagaataat
    21241 gacgcgcaca agggggtata agcaaaggcg gcgtttcaat atggtgcaga tatgagctca
    21301 aatgcctttt atagt
//
LOCUS       DAWISC010000014        21153 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWISC010000014 DAWISC010000000
VERSION     DAWISC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.014"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..144
                     /locus_tag="WIW87_000375"
     CDS             <1..144
                     /locus_tag="WIW87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ5173589.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRH
                     IRT"
     gene            273..1709
                     /locus_tag="WIW87_000376"
     CDS             273..1709
                     /locus_tag="WIW87_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ5173590.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPMI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRRARSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYFAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1720..2703
                     /locus_tag="WIW87_000377"
     CDS             1720..2703
                     /locus_tag="WIW87_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ5173591.1"
                     /translation="MKNFLSLFSSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKLYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTTHTLSGGKDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2700..3893
                     /locus_tag="WIW87_000378"
     CDS             2700..3893
                     /locus_tag="WIW87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ5173592.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRS
                     AAIRQ"
     gene            complement(3953..4129)
                     /locus_tag="WIW87_000379"
     CDS             complement(3953..4129)
                     /locus_tag="WIW87_000379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173593.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFACVLPMPEANAGCV
                     RQTVRGVAMPSEAI"
     gene            4180..5733
                     /locus_tag="WIW87_000380"
     CDS             4180..5733
                     /locus_tag="WIW87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ5173594.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5823..7850)
                     /locus_tag="WIW87_000381"
     CDS             complement(5823..7850)
                     /locus_tag="WIW87_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ5173595.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQRELSEEYGLSVR
                     VDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8139..10145
                     /locus_tag="WIW87_000382"
     CDS             8139..10145
                     /locus_tag="WIW87_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ5173596.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGYPPSDKP"
     gene            complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WIW87_000383"
     CDS             complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WIW87_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ5173597.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRAVEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11286..11537)
                     /locus_tag="WIW87_000384"
     CDS             complement(11286..11537)
                     /locus_tag="WIW87_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173598.1"
                     /translation="MPSDGIFGDDIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPTPGK"
     gene            complement(11579..13006)
                     /locus_tag="WIW87_000385"
     CDS             complement(11579..13006)
                     /locus_tag="WIW87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ5173599.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIKFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDTAAFLLNDIRKDILDCRYCG"
     gene            13191..17330
                     /gene="mfd"
                     /locus_tag="WIW87_000386"
     CDS             13191..17330
                     /gene="mfd"
                     /locus_tag="WIW87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ5173600.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLDDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQPTPSPVGEGWGEGKAVA
                     AQTEFSAAAINPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPLGTSNLHGQIRQQP
                     APSPVGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVA
                     NNLHGQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDS
                     LKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQ
                     YVARSRIHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNE
                     MMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTA
                     AELLNLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMD
                     RLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVA
                     SLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQK
                     EQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGS
                     VREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMR
                     DFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFT
                     LYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPEDYCPDIHERLVLYKRLA
                     VCETVQQINTIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTF
                     GKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLP
                     K"
     gene            17479..17862
                     /locus_tag="WIW87_000387"
     CDS             17479..17862
                     /locus_tag="WIW87_000387"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ5173601.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17884..18726
                     /gene="kdsA"
                     /locus_tag="WIW87_000388"
     CDS             17884..18726
                     /gene="kdsA"
                     /locus_tag="WIW87_000388"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ5173602.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18741..19181
                     /locus_tag="WIW87_000389"
     CDS             18741..19181
                     /locus_tag="WIW87_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ5173603.1"
                     /translation="MMQTFRKISRYVSTLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            19229..20515
                     /gene="eno"
                     /locus_tag="WIW87_000390"
     CDS             19229..20515
                     /gene="eno"
                     /locus_tag="WIW87_000390"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ5173604.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGRVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            20529..20807
                     /gene="ftsB"
                     /locus_tag="WIW87_000391"
     CDS             20529..20807
                     /gene="ftsB"
                     /locus_tag="WIW87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ5173605.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20848..21138)
                     /locus_tag="WIW87_000392"
     CDS             complement(20848..21138)
                     /locus_tag="WIW87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ5173606.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         5056 a         6406 c         5271 g         4420 t
ORIGIN      
        1 aacgtcggta tcggcggcgg cgcgctgctg gggcattggg ttacgcaata ctcgggcatt
       61 tcctgcatcg gcgttgcggg tatgctgacg gcggcggcag gtttgtgggt ctgcctgaac
      121 ctgaaccgcc atatccgaac ctgaccgcac gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccacc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttttgttc caaggatggt ttatgccgct gctgtctgtc gagttcgcac
      301 tgttctttct cgccttcctg ccgatttact ggggcttggc gaaatacccg tccgtccaaa
      361 acctgctgct tttggctgcc ggtatgggct ggctctacca tatcggccct gtatttgcgg
      421 caatcatcgt cctttattcc tcctgcgtgt accttttggg cgaactgctc cgttccgatc
      481 gcgaaaatac gcgccgtttc tggctggggt gcggcattgc cgcctcgctg accgtcttgg
      541 gctttttcaa atatttcgac tttttccgcc cgatgattgc ccaatatgcc ggaaaaggcg
      601 gcgcaatcga catcctgatg ccgctggggc tttcgtatta caccttccag tcgctcgcct
      661 atctggttta ctgcttccgc gccccgcacg ccgcgcgttt cagctggcac gagctgctgc
      721 tgcacctgag ttttttcccc accgttacct ccggcccgat tatccgcgcc gccgcattca
      781 aaagcgcaga cggcgagcag gcaggcgcat tggcgcaaat ccgtacccgc cgagcgcgtt
      841 cgcccgtccg ccccgcactc gccgtttccc tgattctgct gggtattgcc aaaaaatggt
      901 ggctggcggg gatgctggcg gaaaactggg tgtcgcccgt atttgaaaat cccgcccaat
      961 tcgacggctg gggcgtattg ggcggcgtgt acggctatac cttccaactc tttttagact
     1021 tttccggata ttccgatttg gttatcggca tggcgatgct gctgggcttt aggctgccca
     1081 aaaatttctc cgcaccgctt cgtgctttaa acatccgcgc attttgggac aaatggcaca
     1141 tcagcctttc cacctggata cgcgactaca tctacatccc cttgggcggc agcaaaaaag
     1201 gctttttacg gacacagctc aacctgatgg cggcaatggt gctctcaggc atctggcacg
     1261 gctacggctg gaacttcctc atttggggcg cgctgcacgg cacggcactg gtgctgctca
     1321 acacgggcga ccgctatttc ggacgcgacg cgctatgccg tctgaaatac ttcgcgccgc
     1381 tctcatggct cattaccttc catttcgtct gccttagctt tgtcgtcttc aataccgcaa
     1441 atcccgacga tgcaggcgca gttttcagtg ccctctttgc caatgccaac ggctggaatg
     1501 cgccgcaaca ggcaaacatg ctgttgcttg cctcgtttgc atccgtgatg ctgctctacc
     1561 cttacctgca acgcgctttc gacggcgcgg tcaaaggttt ggaaaaaatc ccgatgtggc
     1621 tgtggtttat ccccgtttcc gccgtcctgc tgctgattat cgtcctcgcc ccctcgggga
     1681 tacccggctt tatttatgcc aatttttaag ggtttggaca tgaaaaactt tctttccctt
     1741 ttctcctcca tactgatgtc tgccctgatt gccgtgtggt tcagccaaaa ccccatcaac
     1801 gcctactggc agcagaccta ccaccgcaac agcccgctcg aaccgcttgc cgcctacgga
     1861 tggtggcgga gcggtgcggc gttgcaagaa aacgcctacg ccctttcaga cggcatcaaa
     1921 gccttcctgt ccggcgaaac gccgccgacg gctcaagacg gcggttcggc agatatgccg
     1981 tctgaagccg ccgcatccga agccgtccct caaaccggtg aaacagaatg gaaacaagac
     2041 accgaagccg ccgccgtccg cagcggcgac aaagtctttt ttgtcggcga ctcgctgatg
     2101 cagggcgttg cccccttcgt gcaaaaaagc ctgaaacagc aatacggcat cgaatccgtc
     2161 aacctcagca aacaaagcac ggggctgtcc tacccctcat tcttcgactg gccgaaaacg
     2221 attgaagaaa ccctgcaaaa acatcccgaa atcagcgtac tcgccgtctt cctcggaccg
     2281 aacgacccgt gggatttccc cgtcggcaaa ctctatctca aattcgcttc cgacgaatgg
     2341 gcgcaagaat acctgaaacg tgtcgaccgc atccttgaag ccgcacacac gcaccgcgtc
     2401 caagtcgtct ggctcggcat cccctacatg aaaaaagcca agctcgacgg acagatgcgc
     2461 tacctagaca aactgctttc ggaacatttg aaaggcaaaa tcatcctgat tcccaccacg
     2521 cacaccctga gcggcgggaa agaccgctac accgactccg tcaacgtcaa cggcaaaccc
     2581 gtccgctacc gcagcaagga cggcatacac tttaccgccg aaggacaaaa actgctggcg
     2641 gcaaaaataa tggaaaaaat cgtttttgaa ccaagtacgc aaccatcaag tacacagcca
     2701 tgaaccccaa acacctcatc gcattttccg ccctattcgc cgccacgcag gcagaagccc
     2761 tacctgtcgc ctccgtcagc ctcgacaccg ttaccgtttc cccgtccgcc ccctacaccg
     2821 atacaaacgg gctgctgacc gactacggca acgcctccgc ctcgccttgg atgaaaaaac
     2881 tccaatccgt cgcacaaggc agcggcgaga ccttccgtat cctgcaaatc ggcgactcgc
     2941 ataccgccgg cgacttcttt accgacagcc tgcgcaaacg cctgcaaaaa acttggggcg
     3001 acggcggcat aggctgggtt taccccgcca acgtcaaagg gcagcgcatg gcggccgtcc
     3061 ggcacaacgg taactggcaa agcctcacca gcaggaacaa caccggagac ttcccgctcg
     3121 gcggcatcct cgcccacacc ggcagcggcg gcagcatgac cctgaccgca tcggacggca
     3181 tagcaagcaa gcagcgcgtt tccctgtttg ccaaacccct gcttgccgaa caaaccctga
     3241 ccgtcaacgg caacaccgtc tccgccaacg gcggcggctg gcaggtactg gatacgggcg
     3301 cggcactgcc cctgaccata cacaccgaaa tgccgtggga catcggcttc atcaacatcg
     3361 aaaatcccgc cggcggcatt accgtttccg cgatgggcat caacggcgca caattaaccc
     3421 agtggtcgaa atggcgtgcc gaccgtatga acgacctcgc ccaaaccggc gccgatttgg
     3481 ttatcctttc ctacggcacc aacgaagctt tcaacaacaa catcgacatt gccgacaccg
     3541 aacaaaaatg gctggatacc gtccgccaaa tccgcgacag cctgcctgcc gccggcatcc
     3601 tcatcatcgg cgcacccgaa tccctgaaaa acacgctcgg cgtatgcggc acacgccccg
     3661 tccgcctgac cgaagtccaa cagatgcagc ggcgcgtcgc ccgtcagggg cagacgatgt
     3721 tctggtcttg gcaaaacgcc atgggcggca tatgcagcat gaaaaactgg ctcaaccaag
     3781 gatgggccgc caaagacggc gtacacttct ccgccaaagg ctaccggcgc gcggcggaaa
     3841 tgctcgccga cagcctcgaa gaactcgtcc gctccgctgc aatcaggcaa taatcggaca
     3901 ggaggcggac ggtatttccg caacaggggg atgccgtctg aaacgcatac cttcatattg
     3961 cttcagacgg catagccacc ccgcgcacgg tttgccggac gcaaccggca ttcgcctcag
     4021 gcatcggaag gacgcaggcg aacctccggc atacggcgca aaggcggcgt ttgatatgcc
     4081 gtctgaaggc aaagatgata aactgccgcc ttccgttttc agacggcata ttgttttcaa
     4141 atgagggcgt tctccgtccg caaccataaa ggaagtttca tgaaccggac ttatgccaat
     4201 ttctacgaaa tgctcgccgc cgcctgccgc aaaaacggaa acggcacggc agtgttcgac
     4261 ggcaaggaaa aaaccgccta ccgcgcgctc aagcaggagg ccgaagccgt cgcggcgtat
     4321 ctgcaaaata tcggcgtgaa gttcggcgac acggtcgcgc tggcggtttc caattccaca
     4381 gaatttatta ccgcctattt cgccatctcc gccatcggcg cggtcgccgt accgatgaac
     4441 acatttttga aaaacagcga atacgcgtat atcctgaacg actgcaaggc gcgcttcctg
     4501 ttcgcctcgg ccggcctgtc aaaagaattg gcgggcttga aggcgcaaac gcccgtcgaa
     4561 aaaatcattt ggacggacaa aagccgtccg accggcgaaa cggcggaagg cgatgccttt
     4621 tttgaagacg tgcgccgctt ccccgaaaaa cccgacttgg gccgccaacc ccggataaat
     4681 gatttggcac acatcatcta cacctccggc acgacggggc atcccaaagg cgcgctaatc
     4741 agttacgcca acctgttcgc caacctgaac ggcatcgaac gcatctttaa aatttccaag
     4801 cgcgaccgct ttatcgtttt cctgccgatg ttccacagct tcacgctgac ggctatggtg
     4861 ctgctgccga tttatatggc gtgttcgatt attttggtca aatccgtttt tccgttttcc
     4921 aacgttttga aacagacact gctcaaacgc gcgaccgtgt ttttgggcgt acccgcgatt
     4981 tacaccgcga tgagcaaggc gaaaatccct tggtatttca gatggttcaa ccgcattcgc
     5041 ctgtttatca gcggcggcgc gcctttggcg gaacaaacca tcctcgattt caaagccaag
     5101 ttcccccgcg ccaaattgct ggaaggctac ggactgagcg aagcctctcc cgtcgtcgcc
     5161 gtcaatacgc ccgagaggca aaaagcccgc agcgtcggca tccccctgcc cggtttggaa
     5221 gccaaagccg tcgatgaaga attggtcgaa gtgccgcgcg gcgaagtggg cgaactgatc
     5281 gtcaggggcg gttcggtgat gcggggctac ctcaatatgc ctgccgccac cgatgaaacc
     5341 atcgtcaacg gctggttgaa aacgggcgat ttcgttacca tagacgaaga cggctttatc
     5401 tttatcgtcg accgcaaaaa agatttgatt atttccaaag gtcaaaatgt ctatccgcgc
     5461 gagattgaag aagaaatcta caaactcgat gccgtcgaag ccgccgccgt catcggcgtg
     5521 aaagaccgtt atgccgacga ggaaatcgtc gccttcgtcc aattgaagga aggtatggat
     5581 ttgggcgaga acgaaatccg ccgccacctg cgtaccgtgc tggcaaattt caaaatcccc
     5641 aaacaaatcc actttaaaga cgggctgccg cgcaacgcta cgggcaaggt attgaaacgg
     5701 gtgttgaagg agcagtttga cggaaacaaa tgaacgccgt gccgtccgaa gccccgtccg
     5761 gcaaaaaaat gcggtgaatc tgattcaccg catttttttg acaaacaccg gagggagggc
     5821 atttactctg ccaattccac ctgctcgtgc gccatcagtt cctgaccgac atcatccaac
     5881 aacatcaaat aacgttcgta gtttttcaaa atgtcggcaa tcagctcgtc cttgttcata
     5941 tactgcacat cgtacccgac gcgcccgtcg aaaaaataag cgtagggttt gtaagttgtc
     6001 tgatgccgga tatgcggcag cttgccgtcg ttaatcaact ggtcggatac atcctgcccg
     6061 acagacttaa tcccgtacat aaaatcgcgc atcgtctctt tccgaatgac gaactcgatt
     6121 gcgggctcgt cccgatgaaa cattttatcg acccggacgc tcaagccgta ttcttccgaa
     6181 agctcccgtt gcaactcgtg catagcgggc gatgcagtct gtttgaggaa ttttaaaata
     6241 tcctgctcct gcgtctggct cattatctgc accagccgtt ctttccactt gccgcccgtc
     6301 caaaatacac tggtagggtt aacccgggtc tcaaaatatt tcttatccgc actcaagcct
     6361 ttccacaggc tgaaacacat tatcagcatc agcagggcaa acggcaggga aacaatcagg
     6421 gtcatagact gcaggttgcc gagtccgccc gagcgcatca gcaaaacggc aacggcagac
     6481 atcagcacgc cccacataac cgcctgccac cgtggcgcgc tcaagccttt gtcccgagag
     6541 gtaatattgt tcaggacata aatcccggaa tcggcagaag ttacaaaaaa cagagaaatg
     6601 accagcaggc tgacgatgct cgtcaattcg ggcaggggga ggtaattaaa gaatttaaaa
     6661 agcagcgttt ccggagagga ggtcatcttt tcgagcattc cccccgcaac cccgtcattc
     6721 agccaaatcg ccgtattgcc gaagacggta aaccacaaaa cgccgaacag gccggggatg
     6781 agcaaaaccc cgaagacaaa ctcgcggatg gtgcgcccct ttgaaatgcg cgcgataaac
     6841 aaacccacaa acggcgccca agaacaccac cacgcccaat aaagcaccgt ccaagattca
     6901 aaccacggct tgtgttcccg ttcgtacgca taagttttaa aactgaggcg caccagattt
     6961 ccgaggtagt tccctatgtt gtcgccgaat gccgacaaca ggtaaacagt gggtcccgcc
     7021 gccaaaacaa aaaacagcag caaaaacgca aggcccaggt tcaactcgct caacaccttc
     7081 acgcccttcc ccacgccgga tattgccgaa acgacggcga gggacatgac ggcggcgata
     7141 atcaaaacct gcacgctgaa gctgttttcg gcaatccagc ccatttcctg caatccggcg
     7201 cccagttgcg aagccccgaa ccccaatgtg gtgatgatgc cgaaaaaagt agcaagcaac
     7261 gccataatat caatggcatc gccgaacctt ccggaaattt tttctttcaa cagggggtaa
     7321 aaacaagaac gcagggcaag cggcagcttg tagcggaaac cgaaataagc caaagccaat
     7381 gcaatcgtac cgtacaccga ccaagcgtga acgccccaat ggaacaccgt gtgcagcaat
     7441 gcctgctgct gcctgtgttc cggcgtgccg gccgtaatgt ccgaaaaata atgcatcaac
     7501 ggctctgcca cgccgaaaaa catcagaccc acgcccatcc cggccgcaaa cagcatcgcc
     7561 agccacgaca ggaagccgaa ttccggcaca tcttcatccc gtccgagcct gatgtttccc
     7621 aaactgctga ccgagagtat cagcaggaaa cccagaaaaa tggaaaacgt taaaacataa
     7681 aaccagctga actcggtaaa aatgacttct tttgcccgat cgagccacat ctgcacctga
     7741 tccggcacgg ttaaaaccaa taccaccaaa acacacacaa aaaacaaagt cgtcaaaata
     7801 accatcggat taaatgacgt tcggcgttct ataaattcag acagggacaa accttctcac
     7861 tcctttgtta aaaacagaca aacccggtca tcgggcaaag cggtcaaaac ctgccggtaa
     7921 aataccggct tccggatgac gaaatgcaca aaccggccga aattgttata atcgaaaaaa
     7981 ttaaaaatca atacggatta ttctatcaga ttgatttatc gatatttatt atatcattaa
     8041 tatagtttga tttcaaaccg gcggaaaatg cgccgcccac cgcaggcgcg ccctccccgc
     8101 tatcggggcg tttaacgccg gattgccgat gcggtacaat ggctgatatg aagaaaatta
     8161 cccctcaaaa cctgcgcccc ctgctttcgg aaagcttggg acataccgat tttgtcaacg
     8221 tcctcaacgc actgattaaa tttttgcgcc gtggcggcaa aaaatgtgcg ggggaacgtt
     8281 tcgacctgat tatcgacaca ttcaaacaag acagggaatt actgtcccgc ttcagccggt
     8341 gtttttacat ttggctcgcg caaatacaca tttatccggc actcatcaaa ctcggcatct
     8401 tctcgcgcca cagctttgcc cgggaaatgg gcatacgcat ctacgaacgc ttcagcccgt
     8461 catataaaga ttttgccaac ttgggcgaag tcttccttta tcttttccat tccgaaaacg
     8521 acgacaaatg gctgcaaacg ctcaatatcc gccaatggct ggttttatac gaactcatcc
     8581 ggagccacgc cgagccgtcc aaattgcaga cggcgggcat ccgccttgcc gatgcgcgtt
     8641 tgcgcgccat cgaaatgctg tctgtctgga cggcatccga agccatcgaa cccgacctca
     8701 tccgcatcgc cccgcgcctg ctggaagccg attcttcctt cgtcgccctc caacgcgaaa
     8761 ccgccaaact ggtcgaacac taccgcaacg gcaccacgcc ttacgacacc gcccacctcg
     8821 aagtgatgtt cgaccaatgt ttcagccaga ttgactattt gcgccgcaaa gggacgggcg
     8881 ccggctccgg ttcgtcggtc aaagtcgccc acctgctcga acggctccgg cagaccgtag
     8941 accgtctgaa gctgctcacc gacatccaaa ccggcgccgg caacagcaac cgcctgacca
     9001 tcgcgctgat gaactccctc atctacgcgg cggtcgaaca atacagcacc cgccacctgc
     9061 gccgcagcag catccgtatg ctcgcccgca gcattaccga aaacaaaagc caccacggcg
     9121 aacactacat cacccgcaac cgcaaagaat atttcaaaat gttctactcg gcggcaggcg
     9181 gcggcatcat catcgcccta atggcgctgc tcaaaatccg catcggctca ctcggcctca
     9241 gccccttcct cacttccttg tcggctgggt tcaactacgg catcggcttt atgatcatcc
     9301 atatgctgca ctgcaccgtc gccaccaagc agcccgcgat gactgccgcc agctttgccg
     9361 aacaggtcga tctcaacgaa ggcggcaaag cggtggacaa caaactcgcc aagctcctca
     9421 tcgacgtatg ccgctcccaa agtgtcgccg tcttcggcaa cgtttccatc gccatccttt
     9481 tggcgtgcgc catatcgttc ggctatgccc atctgtaccg gctgcccata ctcgatgccc
     9541 acaccgccgc ctaccagttc aaatccatag acatcatcgc ttacccgacg ctgtggtatg
     9601 ccgccattgc aggtctgtgg ctgttctgct ccggcatcat cgcaggtttt ttcgacaacc
     9661 gcgccgacta cctcaacctg cgccaacgcc tgcccttcaa ccccttgctg cgtaaaatca
     9721 tgcgccccgg gccccgccgc gtcctcgccg cctacatcca caaacactac ggctcgctgg
     9781 tcggcaactt catcttcggg atgctcttgg gtatgaccgg ctatttcgga cacctcctcg
     9841 ggctgccgct ggacatccgc cacgtcgcct tttcctccgc caacctcggc tatgccgccg
     9901 tcagcggcaa cgtcggtttg ggcacgttcg tactcggcat tttcagcgtc ctcgccatcg
     9961 gcctggtcaa cctctgcgtc agcttcagcc tcgccctctt cgtcgccctg cgctcgcgcg
    10021 gcacgaaaat cggcagcatc cgcaatctga ttaaaagttt ttggaatcag attaaaagca
    10081 atccctgcat acttttcctc ccgcccgcca aagaacaggg atatcctcct tcggacaagc
    10141 cttgaccggc aatgccgtct gaagcgggat tcgccccgaa taccgccctg atgcgggaaa
    10201 tccccataaa aggatgcaaa aatgccgtcc gaaccgaaac gtggttcaga cggcatttta
    10261 aaaaacatta caatcccggg ccgccaagcg aattggcaat gtccctgacc gccgttacat
    10321 acatccggct gtggttgtac tgccataccg tataaaaatt gttcaagccc aaataatatt
    10381 caaacacgcc cggtgcggtt tccagtttga acaaaaccgc cttttcatca tctgcaagct
    10441 cttcgccggg gatgatgccg tacgccttca aatccgccac cgtccgcgtc agggcggttt
    10501 tttcgccaat gattgcctga acatccgcac ccggcgccaa tgttgcagac accagcattt
    10561 tcccgcccgt gcgccaaccg tgctgcttca tataattggc aaccgatgcc gcgacatcgc
    10621 cgacgttgcc ccatatgtcc cgatgtccgt ccccgtcata atccaccgcc catttccggt
    10681 agctcgaagg cataaattgc ggcatcccca ttgcgcccgc atagctgcct ttaaaggcga
    10741 aaacatcgcc gccttcttct tttgccagct ttaaaagctc gaccaattct ttttggaaaa
    10801 acccggcgcg gcgggggtaa tcaaagccta aggtcgccaa tgcgtccgcc acacggaaac
    10861 tgcccgtatt tttgccgtaa ttcgtttcaa tcccgataac cgccacgata agttcggcag
    10921 gcacgccgta tttttgcgcc acatcatcga taagcgcgcg gttttccgca taaaaccggc
    10981 gcgcgccgcg aaatttcgcc ttgcccgaat ttcccgtgcg gaacacatac cacggacgcg
    11041 atgtggaggg gcggtgcata atcttgacga tgtccgcctt gtaagccgct ttgtcaaaaa
    11101 aatcctgcca ttccgcccgg gaaaaatccc ctttcccgac ttcatcgtcc acaaaacggc
    11161 ggacatttgc attggcggca aacccgctgt cggataccgg tacggctgcc gcgtcaaata
    11221 cggctgccgc gtcaaacgcg gggcggcttt cttttttcat ttcaaccgcg cggggggctt
    11281 gggcttcatt tgcccggggt gggcgtgcct ccatcgccgt acaggcagac aaagccgcca
    11341 aacaaattgc cagcggcagt atttttctct ttttcataaa tatgttccga acaaataggg
    11401 taagtgggaa agcggcacaa gggggcggcg cgaaatgcgg catccgccgc aatcggcggc
    11461 tttgcggaat gccgcacgtt gcctcttgca ccgcccgaaa tccgtatgtc gtcgccgaaa
    11521 atgccgtctg aaggcacttc ccctttcaga cggcattgcc tgccgccgta tttgcccgct
    11581 acccgcaata tcggcagtcc aatatatctt tgcggatgtc gttcagcagg aaggctgcgg
    11641 tgtcttcgtg tttcgcctgt acgaagacaa agagtcgggc gatgattttg ccgataaaga
    11701 ctttttgcag ggcgcaggca tcggtcaggg tgtgttcctg caggaagctg cggatgtctt
    11761 caggcaaagt gtagtcgcgg gcgacggcgg caaagcgggc atcggtttgc aggcggtcga
    11821 gcagggtttg gtttttgtcc aacttcatgc ctgcttcttt ttcttcggcg gtggcgcgga
    11881 caaactggtc gtaaatttcg gcataaagca tatcgttcgg gccttcaaaa atcgtgaagg
    11941 ggcggatgtc gatagcgata ttgccggcgg tgtgtccgcg ttcaaaaccc ttcgcaccca
    12001 agagtttttg caacatttgc gcggcggcgt aagtgtattc cgtggcgagg gttttgacga
    12061 tgttcgcctc catcagctga tgggcgacgg gggcaacagg cgaaacggaa tggcagacgt
    12121 agcggtaaag aatctcggaa acctgatggc ggcgccggat ttcgcggcgt tcgtaatcga
    12181 cgaatttgat gtcgttgcgg acgtatcgtt ccagattttc aaggatgtat tccataatgc
    12241 cgtgcgtcat gccgatcagt tgcaggcggc tgcggataaa gatgttttgg aacgcgcgca
    12301 aaccggcagc gtcgctctgg gagagtttca tcacggcggt tgcaggcatt tcggcatcga
    12361 tgcggttgac ggcgtaacgg acggcgcgca agccttcgga tgcgagggtt tcgcagcgga
    12421 tgtatgtttt ggggacgagc agcaggtcga tgactttggc gagtttgccg tttttgcgct
    12481 ctttggcggc aacgaggagg aagtcgcttt gcgagttgcc ctgccagtat ttcgcggcgt
    12541 tgacgtaaat ggtttgtccg tcgatatatt cgtagtagga ctgcatttcg cgtgcaatcg
    12601 ccgcgccgga ggtttcgggt tcggtaacac ccaaaccgcc gccctcgcct ttgaaaatca
    12661 tctccaaacc ttgcgcgact tgcgcttcat cgccgaactc ttgcagtggc tgcaacacca
    12721 gcgcgccttc gatgccggta cgcagcgtaa cgggcacgcc gtaatgcccc gcaatccgca
    12781 ggacttcttg gatttcaaac tggctgccct tgcgcccgcc gtattttttg tcgaggaagg
    12841 gcaacagcaa acccgcctgc ttcaaggcaa gccatttgtc ttcgggcagg tatcgcatca
    12901 ggtcgatacc gtctgaaaaa atgcggcgga atgcggattc gatgtgcttt aaaaaagcag
    12961 ccgtgtccat agttgacggc tgcgcgctcg gttcggtgtg tatcatcggc ttcctctgtc
    13021 ggttcccatt aatcggcggc cggtcaaacc gcctgccaca gtttagagtt gattttctaa
    13081 actttaccac aaagtgcgcc gggcaacaat ccgccgacct ttcagacggc atcgcgtccc
    13141 ctcccgtgct aaaatgaccg tttgcatcac tgtccgccga ttgccgcact atgacctacc
    13201 ccatccccaa accccgtgaa aaatcccgtt ggcccaatct ttcgcaaggc tcgctgccct
    13261 tggctttggc gcgttatctg ccgcacaagc ggctcaaggt cgtgctgacc caagatgcgg
    13321 aacaggcgtt gcgccttcag acggcatggc ggtttttccg tccgcacgac acggcggtgt
    13381 tcctgccgga ctgggaaacg ctgccttacg agcgtttttc gccgcatcag gatttggtgt
    13441 cggagcggct gtcggcgttg tggcagatta aaagcggcgc ggcggatgtg ttgttcgtgc
    13501 cggttgccac ggcgatgcag aagctgccgc ccgtgccgtt tctggcaggg cgcacgtttt
    13561 ggctgaaaac ggggcagact ttggatatag gccgtctgaa aagtgatttg gtggatgcgg
    13621 gctacaacca tgtttcccac gttgtcgcgg cgggcgaatt tgccgtgcgc ggcggtatag
    13681 tcgatttgtt cccgatgggc agcgaaatgc cgtaccgcat cgatttgttt gacgatgaaa
    13741 tcgacagcat caaaaccttc gataccgaaa cgcaacgcac catttccccc gtttccgaaa
    13801 tccgcctgct gccggcgcac gagttcccca ccgacagcga ggcgcaaaaa atcttccgca
    13861 gccgcttccg cgaggaagtc gatggtaatc cgaacgatgc ggctgtgtac aaagccgtca
    13921 gcaacggtca tttcggcgcg ggcgtggaat actacctgcc gctgtttttt gaaaacgagt
    13981 tggaaacgct gtttgactat atcggcgaag atgcgctgtt tgtctcttta gacgatgttc
    14041 atgccgaggc aaaccgtttt tggagcgatg tcaaatcgcg ttacgcgatg gcgcagggcg
    14101 acgaaaccta tccgcctttg cttccacagt atttgtatct ctctgccgat gtgttcgcag
    14161 gccgtctgaa aaactacgga caggtgctgc ccgatgtttc cggcaaggaa tacaccctgc
    14221 ccgaccttgc cgtcaaccgc caagcagatg agccgttgca ggcattgaag gattttcaga
    14281 cggcgtttga cggacggatt ttgctgtgcg ccgaaagttt gggacggcgc gaaactatgc
    14341 tcggtttctt gcagcaaaac ggtttgaaag ccaaacccgt gtccgactgg cagggctttt
    14401 tatcggcaca cgagccgctg atgattacag tggcgccgtt ggcatacggg ttcaaactgg
    14461 gcggactgca atcgccgaac caacagcaac ctactccctc ccccgtgggg gagggttggg
    14521 gagagggcaa agcagttgcc gctcaaactg aattttccgc agccgcaata aaccctctcc
    14581 ctagccctct cccacaggag agggaacaaa gtgcagccgc cgtttcagac agtctgaaag
    14641 cagccgccgt ttcaaccgaa agcagcctgc ccctcggtac aagtaatctg cacgggcaaa
    14701 tccgacagca acctgcccct tcccccgtgg gggagggttg gggagagggc aaagcagttg
    14761 ccgctcaaac cgaatttccc gcatccgcaa caaaccctct ccctagccct ctcccacagg
    14821 agagggaaca aagtgcagcc gccgtttcag acgacctgaa aaccaaaagc agcctgcatc
    14881 ccgtcgcaaa taatctgcac gggcaaatcc gacagcaacc tactccctcc cccgtggggg
    14941 agggttgggg agagggcaaa gcagttgccg ctcaaaccga attttccgca gccgcaacaa
    15001 accctctccc tagccctctc ccacaggaga gggaacaaag tgcagccgtc gtttcagaca
    15061 gtctgaaagc agccgccgtt tcaaccgaaa gcagcctgcc ccccggtaaa agtaatctgc
    15121 acgggcaaat ccaacagcaa cctgccccct cccccgtggg ggagggttgg ggagagggca
    15181 aagcagttgc cgctcaaagt gccatcgccg tcatcaccga atccgatctc taccaatacg
    15241 tcgcccgttc gcgcatccac aaccgccgca agaaacacgc cgccgtttca gacgggctgt
    15301 tgcgcgacct tgccgaaatc aatatcggcg accccgtcgt gcacgaagaa cacggcatcg
    15361 ggcggtatat gggcttggta acgatggact tgggcggcga aaccaacgaa atgatgttgc
    15421 tcgaatacgc aggcgaagcg cagctttatg tgcctgtttc gcaactgcat ttaatcagcc
    15481 gctactccgg tcaggcgcat gaaaacattg ccctgcacaa gctcggcagt ggcgcgtgga
    15541 acaaggcgaa gcgcaaagcc gccgaaaaag cgcgcgacac cgccgccgaa ttgctcaacc
    15601 tctacgccca acgcgccgcc caatcgggac acaagtttga aatcaacgag ttggactatc
    15661 aggcgtttgc cgacggcttc ggctacgagg aaaccgaaga ccaggccgcc gccatcgccg
    15721 ccgtgattaa agatttgacg caagcgaagc cgatggatcg ccttgtgtgc ggcgatgtcg
    15781 gcttcggcaa aaccgaagtc gccctgcgcg ccgcgtttgt ggcggtgatg ggcggcaaac
    15841 aggtcgccgt acttgctccg accacgcttt tggtcgagca gcacgcgcaa aacttcgccg
    15901 accgtttcgc cgatttcccc gtgaaagtcg ccagcctttc gcgtttcaac aacagcaaag
    15961 ccaccaaagc cgcgctggaa ggcatggcag acggcacggt cgatattgtt atcggtacgc
    16021 acaaactggt gcaggacgac atcaaattca aaaacttagg tttagtgatt atcgacgaag
    16081 aacaccgctt cggcgtgcgt cagaaagagc agctcaaacg cctgcgcgcc aatgttgata
    16141 tccttaccat gaccgccacg ccgattccgc gtactttaag tatggcgttg gaaggactgc
    16201 gcgacttctc gctgattacc accgcgccca gccgccgcct cgccgtcaaa acctttgtca
    16261 aaccctttag cgaaggcagc gtgcgcgaag ccgtgttgcg cgaactcaaa cgcggaggac
    16321 aggtattttt cctgcacaat gaagtagata cgattgaaaa tatgcgcgag cggctggaaa
    16381 ccctgctgcc cgaagcccgc atcggcgtgg cgcacggaca actgcgcgag cgcgagctgg
    16441 aacaagtcat gcgcgacttt ttgcagcaac gatttaacgt gttgctctgt tccaccatca
    16501 tcgaaaccgg tatcgatatc cccaacgcca acaccatcat catcaaccgc gccgacaaat
    16561 tcggactggc gcaactgcac cagcttcgcg ggcgcgtcgg ccgcagccat caccaagcct
    16621 acgcctacct gctcacgccc gaatacatca ctaaagacgc agaaaaacgc ctcgatgcca
    16681 ttgcggcggc agacgaactc ggcgcaggtt ttaccctagc catgcaggat ttggaaatcc
    16741 gtggtgcagg cgaaatcctt ggcgaaggac aatccggcga aatgatacag gtcggcttca
    16801 cgctctacac cgaaatgctc aaacaagccg ttcgcgacct caaaaaaggc cgccagcccg
    16861 acctcgacgc accgttgggc atcaccaccg aaatcaaact gcacagcccc gccctgctgc
    16921 ccgaagatta ctgccccgac atccacgaac ggctcgtcct ctacaaacgc ctcgccgtct
    16981 gcgaaaccgt gcaacaaatc aacaccatac acgaagaact cgtcgaccgc ttcggcctgc
    17041 ccgaacaacc cgtcaaaacc cttatcgaaa gccaccactt acggcttatg gcaaaagaat
    17101 tgggtatcga tgccattgat gcggccggcg aagcggtaac ggtaaccttt ggtaaaaaca
    17161 ataatgtcga tccaaccgaa atcatcctgc tgattcagaa cgacaaaaaa taccgccttg
    17221 ccggcgccga taagctgcgg tttaccgcag agatggaaaa tatcgaggtc agaatcaaca
    17281 ccgtaaaaaa cgttttaaaa accttgcaaa acagatgcct gcccaaataa agccgacacc
    17341 gcaatgccgt ctgaaacacc gttttccttg tccgaaagcc gccattatga atttgaagga
    17401 aactccacta taatacggca ttcagatttc cagacggcat cgcgcccgtc aaaccgcaca
    17461 caaaccaaaa ggaaatacat gttccgtacc atgcttggcg gaaaaatcca ccgcgccacc
    17521 gttaccgaag ccgatttgaa ctatgtcggc agtattaccg tcgatcaaga cctgttagac
    17581 gcggcaggca tctaccccaa cgaaaaagtc gccattgtca acaacaacaa cggcgaacgt
    17641 tttgaaacct ataccattgc agggaaacgc ggcagcggcg tgatttgtct gaacggtgct
    17701 gcagccaggc tggtacagaa aggcgatatc gtcatcatca tgtcttacgt ccaactctcc
    17761 gaacccgaaa tcgccgcaca cgaacccaaa gtcgtcttgg tagacggaaa caacaaaatc
    17821 cgcgacatca tctcctacga gccgccgcac accgtgctgt aattccgcaa acggacatcg
    17881 attatggata ttaaaatcaa cgacatcacc ctcggcaaca actcgccctt cgtcctattc
    17941 ggcggcatca acgttttgga aagcttggat tccaccctcc aaacctgcgc gcattacgtc
    18001 gaagttaccc gaaaactcgg tattccctat atctttaaag cctctttcga caaggcaaac
    18061 cgttcctcca tccattctta tcgcggcgta ggcttggaag aaggcttaaa gatttttgaa
    18121 aaagtcaaag cagagttcgg catccccgtc attaccgacg tacacgaacc ccatcagtgc
    18181 caacccgtcg ccgaagtgtg cgatgtcatc cagcttcccg cctttcttgc gcggcagacc
    18241 gatttagtgg ttgccatggc aaaaactggc aacgtcgtca acatcaaaaa acctcagttc
    18301 ctcagcccct ctcaaatgaa aaacattgtg gaaaaattcc acgaagccgg caacgggaaa
    18361 ctgattttat gcgaacgcgg cagcagcttc ggctacgaca acctcgttgt cgatatgctc
    18421 ggtttcggcg tgatgaaaca gacttgcggc aacctgccgg ttattttcga cgttacccat
    18481 tccctgcaaa cccgcgatgc cggttctgcc gcatccggcg gtcgtcgcgc acaggctttg
    18541 gatttggcac ttgcaggcat ggcaacccgc cttgccggtc tgttcctcga atcgcacccc
    18601 gatccgaaac tggcaaaatg cgacggcccc agcgcgctgc cgctgcacct tttagaagat
    18661 tttttaatcc gcatcaaagc attggacgat ttaatcaaat cacaaccgat tttaacaatc
    18721 gagtaacacg gtttcgcctt atgatgcaga ctttccgaaa aatcagccgg tatgtctcaa
    18781 ccttgtggct cggtatgcag attatggcgg gttatatcgc cgcaccggtg ctgttcaaaa
    18841 tgctgcccaa aatgcaggcg ggcgaaattg ccggcgtatt gttcgacatc ctctcttgga
    18901 gcgggcttgc cgtttggggc gcggtactgg ctgccgcctt tgccgcccta acccggcggc
    18961 aaaccgccct gctgcttttt ttattgtccg cccttgccgc caaccgattc ttgattacac
    19021 ccgttatcga ggcactgaaa tacggacatg aaaattggct gttgtcgttt gtaggcggat
    19081 ccttcggaat gtggcacggc atttccagta ttgtttttat ggcaaccgcc ctactttcag
    19141 cagttttaag ttggcggctt tccggcaaag atgccgtctg aagccctccc atttttttac
    19201 ctcccttcac ttcacttgga gaacattcat gagcgcaatc gttgatattt tcgcccgcga
    19261 aattttggac tcacgcggca accccacagt cgagtgtgat gtattgctcg aatccggcgt
    19321 aatgggacgc gcagccgtac cgagcggcgc gtccaccggt caaaaagagg ctttggaact
    19381 tcgcgacggc gacaaatccc gttattcggg caagggcgta ttgaaggcgg tcgaacacgt
    19441 caacaaccaa atcgcccaag ccctcattgg tatcgatgcc aacgagcaat cttatatcga
    19501 ccaaatcatg atcgaattgg acggtactga aaacaaaggc aatttgggtg cgaatgcgac
    19561 tttggcggtt tctatggcgg ttgcacgcgc cgctgccgaa gactcaggcc tgccgcttta
    19621 ccgctacttg ggcggcgcag gcccgatgtc cctgcccgta ccgatgatga acgtcatcaa
    19681 cggcggcgaa cacgccaaca acagcctgaa catccaagag tttatgatta tgcccgtcgg
    19741 cgcaaaatct ttccgcgaag cgttgcgctg cggtgcggaa attttccacg ccttgaaaaa
    19801 actgtgcgac agcaaaggct tcccgaccac agtcggcgac gaaggcggtt tcgcccccaa
    19861 cctgaacagc cacaaagaag ccctgcaact gatggtcgag gcgaccgaag ccgccggcta
    19921 caaagcgggc gaagacgtat tattcgcatt ggactgcgcc tccagcgagt tctacaaaga
    19981 cggcaaatac cacttggaag ccgaaggccg ctcctacacc aacgcggaat ttgccgaata
    20041 tctggaaggc ctggtcaacg agttccccat catctccatc gaagacggca tggatgaaaa
    20101 cgactgggaa ggctggaaac tgctgaccga aaaactgggc ggtagagttc aattggttgg
    20161 cgacgacttg ttcgtaacca atccaaaaat cttggccgaa ggcatcgaaa aaggcgtagc
    20221 aaacgcattg ctggtcaaag tcaatcaaat cggtactttg agcgagaccc tgaaagccgt
    20281 cgacttagcc aaacgcaacc gctacgccag cgtaatgagc caccgctccg gcgaaaccga
    20341 agacagcacc attgccgact tggcagtcgc caccaactgt atgcagatca aaaccggttc
    20401 tttgagccgt tccgaccgca tggcgaaata caaccaactg ctgcgtatcg aggaagaatt
    20461 ggcggaagcc gccgactacc ccagcaaagc cgcattctac caactgggca aataaaaaag
    20521 gttaaggtat gaagtgggta actgtcgttt tatccttcgc acttgtctgt tgccaataca
    20581 gcctctggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa cagattgccg
    20641 ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc gccgaagtct
    20701 atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa ctgggttata
    20761 tccaagacgg tgaaaccttt taccgactca tcaggcataa ccggtaatac cgtcaaaaag
    20821 ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca gcccctcatc
    20881 ctcccccgac aaaccgcaat ccagcctgac atccccctcg acgcaacagc agcacggcag
    20941 tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc ttccctccaa
    21001 aatcttcact cggcacgatc cgcaatatcc gcttcggcac tgatattcca ccatatgccc
    21061 cgtccgttcc aagccttcca acagagtctc gccctccaag agttcaaaaa aacccttatt
    21121 cgtaccaatg cgcgccattt ccgaccaatc aaa
//
LOCUS       DAWISC010000015        21101 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWISC010000015 DAWISC010000000
VERSION     DAWISC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.015"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(23..714)
                     /locus_tag="WIW87_000393"
                     /pseudo
     CDS             complement(23..714)
                     /locus_tag="WIW87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1020..1577)
                     /locus_tag="WIW87_000394"
     CDS             complement(1020..1577)
                     /locus_tag="WIW87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173607.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWGYHGANLYNHGKLGTAQGAATATGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1821..1979)
                     /locus_tag="WIW87_000395"
     CDS             complement(1821..1979)
                     /locus_tag="WIW87_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173608.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="WIW87_000396"
     CDS             complement(1976..2818)
                     /locus_tag="WIW87_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5173609.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="WIW87_000397"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="WIW87_000397"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ5173610.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3987..4445
                     /locus_tag="WIW87_000398"
     CDS             3987..4445
                     /locus_tag="WIW87_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ5173611.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4510..5175
                     /locus_tag="WIW87_000399"
     CDS             4510..5175
                     /locus_tag="WIW87_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173612.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5238..6455)
                     /locus_tag="WIW87_000400"
     CDS             complement(5238..6455)
                     /locus_tag="WIW87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ5173613.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6597..7142
                     /gene="def"
                     /locus_tag="WIW87_000401"
     CDS             6597..7142
                     /gene="def"
                     /locus_tag="WIW87_000401"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ5173614.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7229..8155
                     /locus_tag="WIW87_000402"
     CDS             7229..8155
                     /locus_tag="WIW87_000402"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ5173615.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8234..9493
                     /gene="rsmB"
                     /locus_tag="WIW87_000403"
     CDS             8234..9493
                     /gene="rsmB"
                     /locus_tag="WIW87_000403"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ5173616.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQVLKSGGRMLIATCSVFVEENDGQLQKFLNRHA
                     DAELIESRVLLPNKHQDGFYYALIQKQ"
     gene            9459..10055
                     /locus_tag="WIW87_000404"
     CDS             9459..10055
                     /locus_tag="WIW87_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ5173617.1"
                     /translation="MAFITRLFKSSKWLIVPLMLPAFQNVAAEGIDVSRAEARITDGG
                     QLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNIDY
                     KLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAEI
                     RLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10055..12175
                     /locus_tag="WIW87_000405"
     CDS             10055..12175
                     /locus_tag="WIW87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ5173618.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLTVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SETGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12168..13445
                     /locus_tag="WIW87_000406"
     CDS             12168..13445
                     /locus_tag="WIW87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ5173619.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSQ
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13536..14729
                     /gene="dprA"
                     /locus_tag="WIW87_000407"
     CDS             13536..14729
                     /gene="dprA"
                     /locus_tag="WIW87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ5173620.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPVNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITARLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKGIPEKRITAVQTASDQLSLPEGKMPS
                     EKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRY
                     QRIRT"
     gene            14755..15216
                     /locus_tag="WIW87_000408"
     CDS             14755..15216
                     /locus_tag="WIW87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ5173621.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15288..17594
                     /gene="topA"
                     /locus_tag="WIW87_000409"
     CDS             15288..17594
                     /gene="topA"
                     /locus_tag="WIW87_000409"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ5173622.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKC
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCVNYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECLNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17667..18362)
                     /locus_tag="WIW87_000410"
     CDS             complement(17667..18362)
                     /locus_tag="WIW87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173623.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18382..18933)
                     /locus_tag="WIW87_000411"
     CDS             complement(18382..18933)
                     /locus_tag="WIW87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173624.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19052..19429
                     /locus_tag="WIW87_000412"
     CDS             19052..19429
                     /locus_tag="WIW87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5173625.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19518..20087
                     /gene="rsmD"
                     /locus_tag="WIW87_000413"
     CDS             19518..20087
                     /gene="rsmD"
                     /locus_tag="WIW87_000413"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ5173626.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20210..20461
                     /locus_tag="WIW87_000414"
     CDS             20210..20461
                     /locus_tag="WIW87_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ5173627.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20558..20641
                     /locus_tag="WIW87_000415"
     tRNA            20558..20641
                     /locus_tag="WIW87_000415"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20592..20594,aa:Tyr,seq:gta)
     gene            20672..20745
                     /locus_tag="WIW87_000416"
     tRNA            20672..20745
                     /locus_tag="WIW87_000416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20704..20706,aa:Gly,seq:tcc)
     gene            20755..20829
                     /locus_tag="WIW87_000417"
     tRNA            20755..20829
                     /locus_tag="WIW87_000417"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20787..20789,aa:Thr,seq:ggt)
     gene            20878..>21101
                     /locus_tag="WIW87_000418"
     CDS             20878..>21101
                     /locus_tag="WIW87_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5173628.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         5318 a         5345 c         5732 g         4706 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcgtcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      601 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      661 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      721 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      781 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      841 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      901 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
      961 agttattaat gattattttc gagaattgac tgcattgttg gcagcattgg caccaaaacc
     1021 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1081 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgtcgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata cccccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctaggttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaaactt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgcgct ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg tgcaggcggc gagtgccgac agggtgcaga tggggagggg ggttttcatt
     1981 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttggggat tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gttgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctagcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgacgtata accggatacc cgcctgacgc gggtttttta cggaaggggg gcaaaaatgc
     3061 ctaatccgct ttacagacag catatcatct ccatttcgga tttgtcgcgc gaacagttgg
     3121 aatgcctgct tcagacggca ttgaagctga aggcgcatcc gcgcggcgac ctgttggaag
     3181 gcaaacttat cggttcgtgc tttttcgagc cgtccacgcg cacgaggctg tcgtttgaaa
     3241 cggcggtgca gcgtttgggc ggcaaggtca tcggtttctc ggacggcgcg aataccagtg
     3301 ccaaaaaagg cgagacgctt gccgataccg cccgcatcat ttccggatat actgatgcta
     3361 tcatccaacg ccaccccaaa gacggcgcgg cgcgcgtggc agcggagttt tcgcgcgtcc
     3421 ccgttatcaa cgccggcgac ggcacgaacc agcaccccag tcagacgctg ctcgacctgg
     3481 ttaccattta tgaaacacag ggacgtttgg acaagctcaa aatcgccatg gcgggcgact
     3541 tgaaatacgg acgtaccgtg cattcgcttt gtcaggcgtt gaaacgctgg aattgtgaat
     3601 ttgcctttgt ttcgccgccc agcctagcca tgcccgacta tattaccgaa gagttggacg
     3661 aagccggctg ccgataccgt atcctcggta gtttggaaga agcggcggaa tgggcggata
     3721 tcctgtatat gacccgcgtc cagcgcgaac gtttcgacga acaggaattt gccaaaatcc
     3781 aaggcaaatt caacctcgaa gcgtctatgc tcgcccgcgc caaaccgaac ctgcgcgtgc
     3841 tgcaccccct gccgcgcgtg gacgaaatcc atcccgatgt cgatgccacg ccgcacgcct
     3901 attatttcga gcaggcgacc aacggcgttt atgcgcgtat ggcgatattg tcgctggtgt
     3961 tgaacgaaga agtgtgagga accgatatgg aaaccccgaa actcagtgtc gaagccattg
     4021 aaaaaggtac ggttatcgac catattcccg ccggcagggg gctgaccatc ctgcgccagt
     4081 tcaaactttt gcactacggc aacgcggtaa ccgtgggctt caacctgccc agcaaaaccc
     4141 aaggcagcaa agacatcatc aaaatcaaag gcgtgtgctt ggacgacaaa gccgccgacc
     4201 gcctcgccct gttcgccccc gaagcggtgg tcaacaccat cgacaatttc aaggtcgtgc
     4261 agaagcggca tttgaacctg cccgacgaaa tcgccgaagt gttccgctgt ccgaacacga
     4321 attgcgccgg ccacggcgag ccggtcaaaa gccggtttta tgttaaaaag cacaacgggc
     4381 agacgcggct gaaatgccac tactgcgaaa aaacctacag ccgggattcg gtggcggaag
     4441 cctgacggat tcccttaaac cgagtgggcg gcatttcgtc tgccgcctgt tttgccaatc
     4501 tgaaatggaa tgatgatgca cgcttctgtc caaagccgtt tcgcaccgat actttatgtt
     4561 ttgattttct ttgccggttt tttgaccgcg caaatctggt tcaatcagaa agcctatact
     4621 gaagagctgc ctccgcttct gtccgcattg tccgccgtcg cgctggtgtg gctggcgtgg
     4681 gcgttcgtgt cggcgcgttc aaaggccaag gcggaaaagt tctaccgcga aaaaatgata
     4741 cagaacgaaa gcatacaccc cgtcctgcac gcctctttgc aacacttgga acacaagccg
     4801 caaatactcg ccctgctggt caaaaaccac ggcaaaggga tggcggaaca ggtcaggttc
     4861 aaggcggaag tgctgcccga cgacgaagac gcgcgcacga ttgccgccga gttggcaaaa
     4921 atggatatgt tcgcattggg gacggacgcg gtcgcctcgg gcgaaaccta tggacgcgtg
     4981 ttcgccgata ttttcgagtt gtcggcggct ttggaagggc gcgcgttcaa aggaatgttg
     5041 aaactgacgg cggaatataa aaacatcttc ggcgatgcct gccgttcgga aacggcgttg
     5101 gagttgggcg cactcaatca ggcgttgcag gagatttcaa aaacatcgga aaagtccaaa
     5161 cggatatttt attgaagatg gaaaaatgcc gtctgaaacg gaaggtgttt cagacggcat
     5221 ttttgtcgga tgattaatta ttcggagcgg ttgaagccaa acttcacgcg gctgcggccc
     5281 tgatccggta tattgtccaa atcgcgtccc ggattggcgg cggtgtcgcc tacggaaata
     5341 tcggagatgt tttccaaaat gatggcggac gacaggtgtt cggaggtgcg gtaaaccatt
     5401 gccaagccca cttcttcggc aggagtggaa atcagctcga cggtatccct gcttttgaaa
     5461 ttgttggaga ggtcgacctg catcgttttc ttgcgtttgt agaggctcaa aaccgtgcct
     5521 ttgtccaaac cgtccgcctc gcctttgtcg atggtgatgg tttgaaactg gccggcaatc
     5581 cttgtgcctt caaacacgga aacgatttta gcctgaaccg ggcgggacgg ttcgtgcggc
     5641 atcatgttga agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt
     5701 tcggaaatgg cggtttcgac caccagcggc tgtatcgaag gggtgcgcag cggggtaatc
     5761 aaaggatggg tgcgggtatg gtattcgttg tctttcggcc gttctccagc ctgtttcgag
     5821 cgttgttcga ggacagagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact
     5881 tcctgcccga ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc
     5941 aggtagcggc ccggctcttt caggcctttg gtgtaaaccc tggtgccttt ggtgtacagc
     6001 agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg
     6061 atttgcggat gccgcataaa gatgcggtag aagttgacat cgatggcggg aataccgtat
     6121 ccggacactt ccttatccgg actcattttg acgacgggga tgccgtctgt ctgttccaag
     6181 ccgaggcgcg gttcgccgtc aacgtggcgc aacaccaata cctggtccgg ataaatcagg
     6241 tcgggattgt ggatttgatc ccggttcgcg tcccacaggc ggccccattg ccacgggctg
     6301 tacaggtatt tgcccgaaat gccccacagg gtgtcgccct gtttgaccgt gtagcgttcc
     6361 ggcgcgttcg ggcgcacctc caaatttgcc gccaaagttt gtgttgagaa tgccatacct
     6421 gccgcgcaga gcagggttat aatacgacgt tgcataaccg ttccccttat ctgataaatt
     6481 tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc agcccctcgg gatgtgccgc
     6541 gtgataaaaa atgttccgca ttttaacatc gaattatccg caccatcacg gtaattatga
     6601 aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat ggctttattg aatatcttgc
     6661 aatatcccga cgagcgtctg cacacggtgg caaagcctgt cgaacaagtc gacgagcgca
     6721 tccggaagct gattgccgat atgtttgaaa cgatgtacga atcgcgcggc atcgggctgg
     6781 cggcgacgca ggtcgatgtg cacgagcgcg tggtcgtgat ggatttgacc gaagaccgca
     6841 gcgaaccgcg cgtgttcatc aaccccgtca tcgttgaaaa agacggcgaa accacttacg
     6901 aagagggctg cctgtccgtg ccgggcattt acgacaccgt aacccgcgcc gaacgcgtca
     6961 aggtcgaggc tttgaacgaa aaaggcgaaa agttcacgct ggaggcggac ggcttgttgg
     7021 cgatttgcgt gcagcacgag ttggaccacc tgatgggcat cgtgtttgtc gaacgccttt
     7081 cccaactcaa gcaggggcgg attaagacca agctgaaaaa acgtcagaaa catacgattt
     7141 gacccttttg ccgtgccgtc tgaacgctgc aaagttttca gacggcacgg tcttgtccga
     7201 caattttacg cacgcgcagg aacacgctat gaaagtcatc ttcgccggca cgcccgattt
     7261 tgccgccgcc gccttaagag ccgttgccgc cgccggtttt gaaattccgc tggtgctgac
     7321 ccagcccgac cgtccgaaag ggcgcggtat gcaactgact gccccgcccg tcaaacaagc
     7381 cgcgctggaa ctcggtttgc gcgtcgaaca gcccgaaaag ctgcgcaaca acgccgaagc
     7441 cctgcaaatg ctcaaagagg tcgaggcaga cgtaatggtg gttgccgcct acggtttgat
     7501 tctgccgcag gaagtgttgg atacgccgaa acacggctgc ctcaacatcc acgcttcgct
     7561 gttaccccgt tggcgtggcg cggcgccgat tcaacgcgcg attgaagccg gcgatgccga
     7621 gacaggcgtg tgtattatgc agatggacat cggtttggac accggcgatg tggtcagcga
     7681 acaccgctac gccatccaac cgaccgatac cgccaacgaa gtccacgacg cgctgatgga
     7741 aatcggtgcg gcggcggttg ttgccgattt gcaacagctt caaagcaaag gccgtctgaa
     7801 cgcggtcaaa cagcccgaag aaggtgttac ttacgcgcaa aaattgagca aagaagaggc
     7861 gcgtatcgat tggagcaaaa gcgcggcggt tatcgaacgc aaaatccgcg ccttcaaccc
     7921 cgtgcctgcc gcgtgggttg agtatcaggg caagccgatg aaaatccggc gggcggaagt
     7981 ggtggcgcaa caaggcgcgg caggcgaagt gttgtcctgt tcggcggacg gtttggtcgt
     8041 tgcctgcggc gaaaacgcgc tgaagattac cgaattgcag cctgccggcg gcaggcggat
     8101 gaatatcgcg gcgtttgcag caggacggca tatcgaagca ggggcgaagc tgtaaatccc
     8161 ttcagacggc attccgatcc gcaaacggga atgccgtctg aaaccatcag tcgaagaaag
     8221 cgaatcacat aatatgagta tggcacttgc ccaaaaactt gccgccgaca gcattgcggc
     8281 ggttgccgaa ggacgtaacc ttcaggacgt gttggcgcaa atccgcaccg cgcatcccga
     8341 ccttatggcg caggaaaacg gcgcgttgca ggacatcgcc tacggctgcc agcgttattt
     8401 gggcagtttg aaacatatgc tcgcgcagat gctgaaaaag ccgattggca atccgcagct
     8461 cgaaagcctg cttttggcgg cgttgtacca gctgcattac acgcgcaacg cgccccacgc
     8521 cgtggtcaat gaggcggtgg aaagcatcgc gaaaatcgga cgcgggcagt accgttcgtt
     8581 tgccaacgcg gttttgcgcc gctttttgcg cgaacgcgac aagcttgtgg cttcctgtaa
     8641 aaaagacgat gtagcgaaac acaacctgcc gctgtggtgg gtggcttact tgaaaaacca
     8701 ttatccgaaa cactggcaca acatcgccgc cgcgctgcaa tcccatccgc cgatgacttt
     8761 gcgcgtcaac cgccgacacg gcaatgccga aagctatttg gaaaaactgg tggcggaagg
     8821 tatcgcggct aaggcgttgg acgaatatgc ggttacgttg gaagaagccg tgccggtgaa
     8881 ccgcctgcct ggtttttcag acggcattgt ttcggtacag gacttcggcg cgcagcaggc
     8941 ggcgtatttg ttaaacccga aagacggcga acggattttg gacgcgtgcg ccgcgccggg
     9001 cggcaagacg gggcatatct tggaactggc ggattgccgt gttaccgcct tggacattga
     9061 tgcaggccgt ctgaaacggg tggaagacaa tatcgcgcgt ctgggctttc agacggcatc
     9121 gacggcgtgt gccgatgcac aggacctgtc ggcatggtat gatgggaaac cgtttgatgc
     9181 cgtccttgcc gacgtgccgt gtaccgcctc gggcgtggcg cggcgcaatc ccgacgtgaa
     9241 atggctacgc cgtccgaccg acgcgctcaa aaccgcccgc cagcaggaag ccctgctaga
     9301 tgcattgtgg caggtgctga aaagcggggg aaggatgttg atcgctacct gttccgtgtt
     9361 cgtcgaggaa aacgacggac aattgcaaaa attcctcaac cgccatgccg atgcagaact
     9421 gatcgaatcg cgggtactct taccgaacaa acaccaagat ggcttttatt acgcgcttat
     9481 tcaaaagcag taaatggctg attgtgccgc tgatgctccc cgcctttcag aatgtggcgg
     9541 cggaggggat agatgtgagc cgtgccgaag cgaggataac cgacggcggg cagctttcca
     9601 tcagcagccg cttccaaacc gagctgcccg accagctcca acaggcgttg cgccggggcg
     9661 tgccgctcaa ctttacctta agctggcagc tttccgcccc gataatcgct tcttatcggt
     9721 ttaaattggg gcaactgatt ggcgatgacg acaatattga ctacaaactg agtttccatc
     9781 cgctgaccaa ccgctaccgc gttaccgtcg gcgcgttttc gacagactac gacaccttgg
     9841 atgcggcatt gcgcgcgacc ggcgcggttg ccaactggaa agtcctgaac aaaggcgcgc
     9901 tgtccggtgc ggaagcaggg gaaaccaagg cggaaatccg cctgacgctg tccacttcaa
     9961 aactgcccaa gccttttcaa atcaatgcat tgacttctca aaactggcat ttggattcgg
    10021 gttggaaacc tctaaacatc atcgggaaca aataatgcgc cgttttctac cgatcgcagc
    10081 catatgcgcc gtcgtcctgt tgtacggact gacggcggca accggcagca ccagttcgct
    10141 ggcggattat ttctggtgga ttgttgcgtt cagcgcaatg ctgctgctgg tgttgtccgc
    10201 cgttttggca cgttatgtca tattgctgtt gaaagacagg cgcgacggcg tattcggttc
    10261 gcagattgcc aaacgccttt ctgggatgtt tacgctggtt gccgtactgc ccggcgtgtt
    10321 tctgttcggc gtttccgcac agttcatcaa cggcacgatt aattcgtggt tcggcaacga
    10381 tacccacgag gcgcttgaac gcagcctcaa tttgagcaag tccgcattga atttggcggc
    10441 agacaacgcc ctcggcaacg ccgtccccgt gcagatagac ctcatcggcg cggcttccct
    10501 gcccggggat atgggcaggg tgctggaaca ttacgccggc agcggttttg cccagcttgc
    10561 cctgtacaat gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca
    10621 gccgtttcca ggtaaggcgc gttgggaaaa aatccaacgg gcgggttcgg tcagggattt
    10681 ggaaagcata ggcggcgtat tgtacgcgca gggctggctg tcggcgggta cgcacaacgg
    10741 gcgcgattac gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt
    10801 cttaatcgaa aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca
    10861 gacctttttc ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt
    10921 catggcactg tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc
    10981 gaaggcggtg gcgcaaggcg atttcagcca gacgcgcccc gtgttgcgca acgacgagtt
    11041 cggacgcttg accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc
    11101 agacgagcgc aaccgccggc gcgaggaagc cgccaggcat tatcttgaat gcgtgttgga
    11161 ggggctgacc acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa
    11221 agcggcggaa cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg
    11281 ttggcacggc gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc
    11341 ggcggcaggt acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat
    11401 cctgctgggc aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat
    11461 tgacgacatc accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcgaa
    11521 gcggctggca cacgaaatcc gcaatccgct cacgcccatc cagctttccg ccgaacggct
    11581 ggcgtggaaa ttgggcggga agctggatga gcaggatgcg caaatcctga cgcgttcgac
    11641 cgacaccatc gtcaaacagg tggcggcatt gaaggaaatg gtcgaagcat tccgcaatta
    11701 tgcgcgttcc ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt
    11761 gttggcattg tatgaagccg gtccgtgccg gtttgcggcg gagcttgccg gcgaaccgct
    11821 gacggtggcg gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc
    11881 cgccgaagcg gcggaagaag ccgatgtgcc cgaagtcagg gtaaaatcgg aaacagggca
    11941 ggacggtcgg attgtcctga cggtttgcga caacggcaaa gggttcggca gggaaatgct
    12001 gcacaacgcc ttcgagccgt atgtaacgga caaaccggcg ggaacgggat tgggtctgcc
    12061 tgtggtgaaa aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc
    12121 gggtggcgcg tgtgtcagaa tcatcttgcc aaaaacggta aaaacttatg cgtagcagcg
    12181 atattttaat tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg
    12241 acgaaggtta ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc
    12301 aggcgcgccc cgcgatggtg ctgctggata tttggatgcc tgattgcgac ggcatcaccc
    12361 ttttgaagga gtgggcgaaa aacgggcagc tcaatatgcc ggtggtgatg atgagcgggc
    12421 atgccagcat cgataccgcc gtggaagcca ccaaaatcgg cgcgatcgat tttttggaaa
    12481 aaccgatttc cctgcaaaag ctgctgtctg ccgtcgaaaa cgcgttgaag tacggtgcgg
    12541 cgcaaaccga aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaaa
    12601 tgaaccgtga ggtaggggct gcggtgaaat gtgcctctcc cgtacttttg acgggcgagg
    12661 cgggttcgcc gtttgaaacg gtggcacgct atttccataa aaacggtacg ccgtgggtca
    12721 gcccggcaag ggtcgaatat ctgatcgata tgccgatgga actgttgcag aaggcggagg
    12781 gcggcgtttt gtatgtcggc gacatcgccc agtacagccg caacatccaa gccggtattg
    12841 cctttattgt cggaaaggcg gaacaccgcc gcgtcagggt ggtcgcatcg ggcagcaggg
    12901 cggcaggttc agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg
    12961 tcgtccgtat tccgccgctg cgtatgcagc atgaagacat tcccttcctg atacagggga
    13021 ttgcctgcaa tgtggcggaa agccaaaaga ttgcgcctgc ctcattcagt gaagaggcac
    13081 ttgccgcatt gacccgttac gactggccgg gaaatttcga ccaactgcaa agcgtcgttg
    13141 caacgctgtt gttggaggcg gacggacagg aaatcggcgc aggggcggtt tcttcccttt
    13201 tggggcagaa tgtgcctgcc gagggggcgg aagatatggt gggcgggttt aatttcaacc
    13261 tgcccctgcg cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc
    13321 aagaaggtca gaatatgagc caagtggcgc agaaagttgg tttggaacgc acgcaccttt
    13381 accgcaaact caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag
    13441 aataggcccg gacggccggt ttaccggctg cgggcttttg ttttcagacg gcatttggtg
    13501 caaatgccgt ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt
    13561 ggctgcaatt ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc
    13621 gtttcggcag cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac
    13681 ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg
    13741 cggcagaagc ggcattggaa tgggaaatgc gggacggatg ccgcctgatg ctgcttcagg
    13801 atgaagattt tcccgaaatg ctgacgcagg ggctgaccgc gccaccggtt ttgtttttgc
    13861 gcggcaacgt gcaactgctg cacaaacctt ccgccgccat cgtcggcagc cgtcatgcca
    13921 cgccgcaggc gatgcggatt gccaaagatt tcggcaagtc gttgggtggg aaaggcattc
    13981 ccgttgtgtc gggtatggct tcgggcatcg ataccgccgc ccatcagggt gcgttgcagg
    14041 cagaaggcgg caccatcgcc gtgtggggga cgggcataga ccgcatttat ccgccggtca
    14101 acaaaaacct tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag ttccccatcg
    14161 gcacgcggcc gtatgccggc aattttccgc gccgcaaccg cctgattgcc gccctgtcgc
    14221 aagtaacgct ggtggttgaa gccgcgttgg aatccggttc gctgattact gccagattgg
    14281 cggcggagat ggggcgcgaa gtgatggcgg tacccggctc gatagacaat ccacacagta
    14341 aaggctgcca caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc
    14401 tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg
    14461 gaatacctga aaagcgcatc actgccgttc agacggcatc cgaccagctg tctctgcctg
    14521 aaggcaaaat gccgtctgaa aagacggaga accgacccgt cggcggcagt atcttggaca
    14581 ggatgggttt cgacccagtt catcccgacg tgcttgccgg acagttggct atgcctgccg
    14641 cagatttgta tgccgcactg ttggaattgg aattggacgg cagcgttgcc gcaatgcccg
    14701 gcggcagata ccagcgtatc cgaacttgaa cgcactttat attaaggaac acgaatgacc
    14761 gaagtcatcg cctacctcat cgaacatttc caagatttcg atacctgccc gccgcccgaa
    14821 gacttgggta tgctgcttga agaagcgggt ttcgatacga tggaaatcgg caacaccctg
    14881 atgatgatgg aagtattgct caacagctcc gaattttccg ccgaacccgc cgacagcggc
    14941 gcattgcgcg tgtacagcaa agaagaaacc gacaacctgc cgcaggaagt gatggggctg
    15001 atgcagtatc tgattgaaga aaaagccgtc agctgcgaac agcgggaaat catcatccac
    15061 gcgctcatgc acattccggg cgacgaaatt accgtagata ccgccaaagt gctgaccctg
    15121 ctgcttttat gggcaaacaa gagcgagctg cccgtgttgg tcggcgacga gctgatgagc
    15181 gcgcttttac tcgacaacaa acccacgatg aactgaagcg gcttcagacg gcccgcccga
    15241 gtccgtctga aacgtcggca tcaaaaccac catccagaga acgacaaatg gcgaaaaacc
    15301 tattaatcgt cgaatccccg tccaaagcca aaaccctgaa aaaatatttg ggcggcgatt
    15361 ttgaaatcct tgcatcctac ggacacgtcc gcgacctcgt ccccaaaagc ggcgcggtcg
    15421 atcccgacaa cggctttgcg atgaaatacc aactcatcag ccgcaacggc aaacacgtcg
    15481 atgccatcgt cgccggtgcc aaagaagctg aaaacatcta cctcgccacc gacccggata
    15541 gggaaggcga agccatttcc tggcatcttt tggaaatcct caaatccaaa tgcggcttga
    15601 aaaacatcaa gccgcagcgt gtcgtgttcc acgaaatcac caaaaacgcc gtgctcgatg
    15661 ccgttgccca tccgcgcgaa atcgaaatgg acttggtcga tgcgcaacaa gcccgtcgcg
    15721 ctttggacta tttggtcggt ttcaaccttt cgccattgtt gtggaaaaaa atccgtcgcg
    15781 gtttgagcgc gggccgtgta caaagccccg cactgcgttt gatttgcgaa cgcgaaaacg
    15841 aaatccgcgc gtttgaagcg caggaatatt ggacggtaca tctagacagc cacaaaggcc
    15901 gcagcaagtt caccgccaaa ctcgcccaat acaacggcgc gaaactcgaa caattcgacc
    15961 tgccgaacga agccgctcaa gccgatgtgt tgaaagaact cgaaggcaaa gaggccgtcg
    16021 ttaccgccat cgaaaagaaa aagcgcagcc gcaaccccgc cgcgccgttt accacatcca
    16081 ccatgcagca ggatgctgtg cgcaaactcg gcttcaccac cgaccgcacc atgcgtaccg
    16141 cccagcagct ttacgaaggt attgacgtag ggcagggtgc catcggtctg attacctata
    16201 tgcgtaccga cagcgtgaac ttggcggatg aagccttaac cgaaatccgc cattacattg
    16261 aaaacaaaat cggcaaagaa tatctgccga gtgccgccaa acaatacaaa accaaatcca
    16321 aaaacgccca agaagcgcac gaagccatcc gcccgacttc cgtgtaccgc acgcccgaaa
    16381 gcgtcaaacc cttcctgagc gccgaccagt tcaaactcta tcaaatgatt tggcagcgta
    16441 ccgtcgcctg tcagatgacg cccgccaaat tcgaccaaac caccgtcgat attaccgtcg
    16501 gcaaaggcgt attccgcgta accggacaag tgcaaacctt cgcaggcttc ctcagcgttt
    16561 acgaagaaag cagcgacgat gaagaaggcg aagacagcaa aaaactgccc gaaatgagcg
    16621 aaggcgacaa attgcccgtg gacaaactct acggcgaaca acactttacc actccgccgc
    16681 cacgctacaa cgaagccacg ctggttaaag ccctcgaaga atacggcatc ggccgcccct
    16741 cgacctacgc cagcatcatc tccacgctca aagaccgcga atacgttacc cttgagcaaa
    16801 aacgctttat gcccaccgac acaggcgaca tcgtcaataa attcctgacc gaacacttcg
    16861 cccaatacgt cgattaccac ttcactgcca aactcgaaga ccagcttgac gaaattgccg
    16921 acggcaaacg ccaatggatt cccttgatgg acaaattctg gaaaccgttc atcaaacaag
    16981 tggaagaaaa agaaggcatc gaacgcgcca aatttaccac gcaggaactt gatgaaacct
    17041 gcccgaaatg cggcgaacac aaactgcaaa tcaaattcgg caaaatgggt cgttttgttg
    17101 cgtgtgccgg ttatcccgag tgcagctaca cgcgcaatgt caacgaaacc gccgaagaag
    17161 ctgccgaacg catcgccaaa gccgaagccg aacaggccga actcgacgga cgcgagtgcc
    17221 cgaaatgtgg cggtcgccta gtgtacaaat acagccgcac cggcagcaaa ttcatcggct
    17281 gcgtcaacta tccgaaatgc aaacacgtcg agccgctgga aaaaccgaaa gataccggcg
    17341 tccagtgtcc gcaatgcaaa aaaggcaacc tcgtcgagcg caaatcccgc tacggcaaac
    17401 tgttttacag ttgcagcacc tatcccgact gcaactacgc cacttggaac ccgcccgttg
    17461 ccgaagaatg cctgaactgc cattggccgg tcttgaccat caaaaccact aaacgctggg
    17521 gtgtagaaaa agtctgccca caaaaagaat gcggctggaa agaacagatt gaaccgcccg
    17581 cgccgaagga gtaagattag gttggtttga aagagaaaag gtcgtctgaa aaattttcag
    17641 acgacctttg cttttctgtg attggtttat ttgaatccgc gtgttgtttt aaagtccgat
    17701 aaaatccggt tcatttcagg cgcaaacaag gcgatgtaat cgtaagatag accgcgactg
    17761 gcactgggat ggggaaagca gacgacttcg caatcttcaa acgattggaa tttgacattg
    17821 aaacgtgtac cgtcaaattc tttttgcacc gtctccagcg gtttggtctg cttaccgacc
    17881 aactgctcga agcgtggcag tacattttgg ttgttcagaa aatccgccaa cctgctgccc
    17941 atgaagagga tgactttcgg acgcagtttt tcgatgtggt agagaaaatt atcgatgtgc
    18001 tcgggttgtg tgaacttgtc gggattgtcg atagtgttgc cctgtgtagc agcccagttg
    18061 gtttgaacca gggatttttc aaatgcaccg cccaatccat tttcgtctaa ggggtgtccc
    18121 cacatttcaa accaattttt tatcgtattg tcgtaacgcc acttttttgc ctgctctccg
    18181 aaatagaggg atttgtttgc aaatgtatgg tcgattttgt tttcagggag tttgtattca
    18241 cctgctacat aagcagcctc atcggcttta ctccaacccc attcatagcc acaaatcatt
    18301 aagccatgtt tgtcgttgta gcctttgaac aggctgttgc tcaaattcaa atccttcatc
    18361 atgaactctt ccttttaaaa tttaagagcg attgacttca atgtttttag atggggtgga
    18421 aaaatccttg tgtaggcaac ataaattcaa taaatttctt gataattcga aacctactaa
    18481 tagcgcacct ataaaagctt tttcattacg ttcagcatga cggtcacgtc gttcatattt
    18541 tttacgcttg ctgttccctg ttattacagc taagccaagt gatatggcga gaattgccca
    18601 aacaatagta cttaataaca attttcccca tactatcaat aaggaaagaa aaaacctttt
    18661 agtattagat cgataggtta taatccatgc ccatgaaaat gcaataagag ttatacataa
    18721 gatacaagct gacaggattt ctttattttt aattaaataa cttagagcga tgaggatgac
    18781 aggtgtcaag aaaataatag ttacatcccg atagctataa aagaaaactg ccctattttg
    18841 aatgtggaga tgtgcacaga atccaatata gctaaggata atagttaata taataaaaaa
    18901 agaccaccaa gggtgaagag ataggaattc catgttttcc ctttttttgt aaaaaggaaa
    18961 aaatcttatc taatagttaa ttgcctaacc agccagaaga agtttaaaat ctatcccaat
    19021 aattcaacca tctatacaga aagttcagct tatggaaacc cacgaaaaaa tccgcctgat
    19081 gcgcgaattg aataaatggt cccaggagga tatggcggaa aagctggcga tgtcggcagg
    19141 cgggtatgcc aaaatcgaac ggggcgaaac gcagttaaat atcccgcgtt tggagcagtt
    19201 ggctcagatt ttcaaaatcg atatgtggga cttgctcaaa tcgggcggtg gtgggatggt
    19261 gtttcagatt aatgaaggtg atagtggtgg cgatattgcg ttgtatgcgt cgggtgatgt
    19321 ttcgatgaaa atagaatttt taaaaatgga gttgaaacac tgcaaagaaa tgttggaaca
    19381 aaaagacaaa gaaatcgagc tgctccgcaa gctgaccgaa accgtttaaa cagatatgcc
    19441 gtctgaaaaa agttttcaga cggcatattc tttgacaggt cttgtataat accgtttgaa
    19501 cttacaggtt tttgattatg gcggcaggca aacataccaa acacagcaac cgggtacgca
    19561 ttatcggcgg gcaatgccgg ggcaggaaat tgagtttcac atccgccgac ggactgcgtc
    19621 cgacacccga cagcgtgcgt gaaaagctgt ttaactggct gggacaggat ttgacgggta
    19681 aaacggtttt ggatctcttc ggaggcagcg gcgcactcgg tatagaagcc gcttcgcgca
    19741 acgccaaacg cgtgctgatt tcggataaca accgccaaac cgtgcagacc ttgcagaaaa
    19801 acagtcgcga actgggtttg gggcaggtgc aaatcgtctt ttcagacggc atcgcatatt
    19861 tgaagaccgt atccgaacag tttgatgttg tctttctcga cccgccgttt gcatggcagg
    19921 actggcaaat cctgttcgat gccttgaagc cgtgcctgaa cccccgggca ttcgtctatc
    19981 tcgaggcggg tacgctgccg aatattcccg attggctgac ggaatataga gaagggaaat
    20041 cggggcagag tacatttgaa ttaagggttt tccaagtggc tgaataatat gcgctttgat
    20101 aatcatttcc gagttgtaaa cattcgtttg caaccgtccg gttcaaaaaa accttgtgct
    20161 ataatccgcg cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa tgtcgctttt
    20221 tattaccgac gagtgcatca actgcgacgt atgcgaaccc gaatgcccca atgatgccat
    20281 ttcccaaggc gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt gcgtcggaca
    20341 ctacgatgag ccgcagtgcc agcaggtttg cccggtggac tgcatcctga ttgacgaaga
    20401 acatcccgaa acccatgacg agttgatggc gaaatacgaa aagattatcc agtttaaata
    20461 aattcttttt aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac
    20521 ggaaaagcaa gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa
    20581 gggggcagac tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc
    20641 aaaattctta cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag
    20701 ccttccaagc ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat
    20761 gtagctcagg ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca
    20821 tgggcaccat ctctcgatta ttcatttctt taaggcttag atatatagga tattgccatg
    20881 gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt
    20941 gaccatggta aaaccaccct gactgccgct ttgactacta ttttggctaa aaaattcggc
    21001 ggtgctgcaa aagcttacga ccaaatcgac aacgcacccg aagaaaaagc acgcggtatt
    21061 accattaaca cctcgcacgt ggaatacgaa accgaaaccc g
//
LOCUS       DAWISC010000016        20709 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWISC010000016 DAWISC010000000
VERSION     DAWISC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.016"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(63..239)
                     /locus_tag="WIW87_000419"
     CDS             complement(63..239)
                     /locus_tag="WIW87_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173629.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1609)
                     /locus_tag="WIW87_000420"
     CDS             complement(242..1609)
                     /locus_tag="WIW87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ5173630.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GERFDAHDFVEDVLAAGAAAVVVSREDCAAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPNAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     IALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGELGEDEAAAMHAEVGAYARDQGIE
                     AAYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVE
                     ALEDK"
     gene            complement(1734..3382)
                     /locus_tag="WIW87_000421"
                     /pseudo
     CDS             complement(1734..3382)
                     /locus_tag="WIW87_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3405..4883)
                     /locus_tag="WIW87_000422"
     CDS             complement(3405..4883)
                     /locus_tag="WIW87_000422"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ5173631.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVAGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAVRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4908..6653)
                     /locus_tag="WIW87_000423"
     CDS             complement(4908..6653)
                     /locus_tag="WIW87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ5173632.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6714..6977)
                     /gene="ftsL"
                     /locus_tag="WIW87_000424"
     CDS             complement(6714..6977)
                     /gene="ftsL"
                     /locus_tag="WIW87_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ5173633.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6974..7960)
                     /gene="rsmH"
                     /locus_tag="WIW87_000425"
     CDS             complement(6974..7960)
                     /gene="rsmH"
                     /locus_tag="WIW87_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ5173634.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7957..8412)
                     /gene="mraZ"
                     /locus_tag="WIW87_000426"
     CDS             complement(7957..8412)
                     /gene="mraZ"
                     /locus_tag="WIW87_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ5173635.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8691..9902
                     /locus_tag="WIW87_000427"
     CDS             8691..9902
                     /locus_tag="WIW87_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5173636.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPSPEARQFSLKLQTLIAE
                     KIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQL
                     QELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSA
                     RPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKL"
     gene            10034..10110
                     /locus_tag="WIW87_000428"
     tRNA            10034..10110
                     /locus_tag="WIW87_000428"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10068..10070,aa:Met,seq:cat)
     gene            10118..10193
                     /locus_tag="WIW87_000429"
     tRNA            10118..10193
                     /locus_tag="WIW87_000429"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10151..10153,aa:Ala,seq:ggc)
     gene            complement(10475..11296)
                     /locus_tag="WIW87_000430"
     CDS             complement(10475..11296)
                     /locus_tag="WIW87_000430"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ5173637.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11329..11973)
                     /locus_tag="WIW87_000431"
     CDS             complement(11329..11973)
                     /locus_tag="WIW87_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5173638.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11976..12839)
                     /gene="ftsN"
                     /locus_tag="WIW87_000432"
     CDS             complement(11976..12839)
                     /gene="ftsN"
                     /locus_tag="WIW87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ5173639.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMG
                     AYADRQSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12945..14441)
                     /locus_tag="WIW87_000433"
     CDS             complement(12945..14441)
                     /locus_tag="WIW87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ5173640.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDMEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14455..14775)
                     /locus_tag="WIW87_000434"
     CDS             complement(14455..14775)
                     /locus_tag="WIW87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ5173641.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLEKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15258..16784
                     /gene="putP"
                     /locus_tag="WIW87_000435"
     CDS             15258..16784
                     /gene="putP"
                     /locus_tag="WIW87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ5173642.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            complement(16874..17041)
                     /locus_tag="WIW87_000436"
     CDS             complement(16874..17041)
                     /locus_tag="WIW87_000436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173643.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17033..20638
                     /gene="putA"
                     /locus_tag="WIW87_000437"
     CDS             17033..20638
                     /gene="putA"
                     /locus_tag="WIW87_000437"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ5173644.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRTQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4850 a         5968 c         5047 g         4844 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaattgggt aactccccgg atttttaccg
      121 ccgcccaagt ctaaacataa atcttcataa agatacgcct gcgtccgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcagcaat aaggctttaa aaaaacgtcg gcttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcatacgc gccgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca gttcgcccat atcgcccatc acgaaaatac
      541 gcggcgcagg catacgcgcc aacacgtcaa tcgcagcttt catgctgtca gggttcgcat
      601 tataagtatc gtcaatcagg gttgcgccct tgattccgga tttgacgttc agacggcctt
      661 tgatattgct gaagcctttc aaaccttccg ccacatcgtt caaactcaaa cccgcagcca
      721 aagccagcgc ggcggcagcg gcggcgttgt ggacattgtg gcggccggga acaggcagca
      781 ccacggcggc gcgctcatcg ccgcacacca aatcaaattc gcacgacaac ggtttcagca
      841 caatattttc cgcgtgaaca tcgccgctat cgatgccgaa agtgcgcgta ttcaaattaa
      901 gcgttgccgt tttgaagaca gccatattgg catcttcttg aggaatcagt gcaatgccgt
      961 ctgaacataa accttggtaa atctcgcttt tcgctttggc aatatcgccc actccgtcga
     1021 aaccgcagcc gacatgggcg cgcatggcgt tgttgaccaa tgcggcattt ggtttggcga
     1081 tttgcgttaa aaccgccagt tcgccgaaat ggttcatgcc catttcaatc acggcatagc
     1141 ggtgtttttc gtttaacttc aacaaagtca gcggcaatcc gatatggttg ttgaagttgc
     1201 ctgccgtcgc caacacggca tcatcgccga aacggcggcg caataccgca gccagcattt
     1261 ccttcaccgt cgtcttgccg cccgaaccgg taatgccgaa cacaaacgga ttcacatttt
     1321 cacgccacgc ctttgccagc gtttgcaatg cggcaagcgt gtcatcgact ttcaacgcgc
     1381 catccattgc agcacaatct tcgcgcgaaa ccacaaccgc cgccgcacca gcagccaata
     1441 cgtcttcaac aaaatcatgc gcgtcaaacc gctcgcccgc caatgcgaaa aacacatcgc
     1501 ccgcgcggat gtcgcggctg tcggttacga tgcgcgacac gggtttgctt tcagacggca
     1561 tcggaagctt gagggcttgg cagatgaaat ttaggtccag tggtttcata tttactttcg
     1621 ttaatattcg ggcggcggac acatcggtag cggctgattt ttttatcgcc tgttttgctg
     1681 tggtaaaaca cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt
     1741 ttagacgtat ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc
     1801 agtccgtcga taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa
     1861 aaatcatagc cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg
     1921 cgtaaaaaat taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac
     1981 gaaattttaa ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt
     2041 cgctcgccgc ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag
     2101 ctttctttat tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg
     2161 atggtggaaa catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta
     2221 tccaaacgtg tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc
     2281 ttcgtgcctg catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc
     2341 aaccccgcgc ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg
     2401 gcataggtgg aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc
     2461 gtccgaaaac ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc
     2521 cccaaagtct gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc
     2581 aacagccctt tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc
     2641 atataatccg aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca
     2701 acatgacgga tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg
     2761 gcggcatatt gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac
     2821 aggccggcat caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag
     2881 gacaaaatca aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt
     2941 acgtcagcaa ataccccccc cccgatacac aatatttcca aactgtcaag ccaaaaatga
     3001 aaaataatgc ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct
     3061 cggcaggatt gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc
     3121 agccgaccgg caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa
     3181 cgcgcgggaa accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg
     3241 cagaataacg gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa
     3301 aacaaaaggc taaaagcgcg caaatgccca atgtattttt taatgtctga ttcatatttt
     3361 ttattcgggc ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac
     3421 aaagcctgtc cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga
     3481 tagttttcat gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc ttgttcaacc
     3541 gcataacgga cggcggcggc acggtcggct tcgacgcatt cgggcgcggg aacggcaggc
     3601 aggatgtcgt tgatgatgtc gtgcggattt tccaaacgcg ggttgtcgct ggtgacgacg
     3661 actttatccg cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc gcgatcgcgg
     3721 ttgccgccgc aaccgaatac gcaccataaa gccgcaccct gcggtttgat ttcctgcaag
     3781 gtggcgagtg ctttttccaa tgcgtcgggc gtgtgggcat aatcgacaac gaccaagggc
     3841 ttgccgctgt tcatgatgca gtccatgcgc cctgaagcgg gacggatttt tgccagcaca
     3901 tccaatacct tatcaagcgg atagccgttg gcgcacagca aggcgatgca ggcggcgagg
     3961 ttttgcgcgt tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc ccacggggtt
     4021 tggaatacgg ctgctatgcc gtctgaagag gcggtaaagt cggtaatgcg gatgtcggcg
     4081 tgttcgctga aaccatagct gtaaacggcc aaatcgggac agtctttttt cagacgacct
     4141 acgagttccg cgccgtattc gtcatccacg ttgatgactg cgtgtttcaa gccgtgccag
     4201 taaaacaggc gcgacttgat ggcaccgtag gcttccatcg tgccgtggta gtcgaggtgg
     4261 tcgcgggtga ggttggtaaa gattgcgctg cggaatgaca cgccgttgac gcgcgactgg
     4321 tcaagcccgt ggctggagac ttccatcgcg gcgactgttg cgccttgttg acggaaacgg
     4381 tagagcaggg tttggacatc gacgggggcg ggtgtggtat gcgtggtttc ttccaatgca
     4441 ccccaaaagc cgttgccgac cgtgccgaca atggcggttt tttcgcccaa caaatcggca
     4501 gcttgcgcca gccattgtgt gatggaggtt ttgccgttgg ttccggctac gccccaaact
     4561 ttgaggccgt ctgaaacgtt gccgtaaact tgcgccgcca atatgccggc acggtgtttc
     4621 aaatctttga tgccttgatt ggggactttc cattcgggat tccacgcaaa tttgccgtcg
     4681 tcgtcccaaa aaacaaaagc cgcgccgttg gcaacggcgg cggggatata actgcgtccg
     4741 tcggcatatt cgcccggaca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct
     4801 gaatgcaaca aacgccctgc cgcgtttgca cacgacagag tcgggatgcc ggtttcagcc
     4861 aaaggggtta acttgctgaa cataaaacaa tctcgttgat actcggatta agacggtgtt
     4921 ttgacggctg cggcggtcag tggcttggtc ggggaaatgc ccaagatgtt caggctgccg
     4981 cccataattt ttttgaaggg cggccctgcc actacgccgc cgtaataacc gtgggcagtc
     5041 ggttcgtcaa tggttaccgc cacaatcaca cggggatttt tggcgggggc aaaaccgata
     5101 aaggtagcga tgtgtttgtt gtcggcataa cgcccgttga cgaacttgcg cgccgtgccg
     5161 gttttcgcgc cgacatcgaa accgtccacc gcacccgccg taccggtgcc gcccggctcg
     5221 gttacggaaa ccatcagatt gcgtacctcg cgcgcggtcg attctttgaa tatgcgtttg
     5281 ccttgcggcg caaccgcctg tttttcaaag ctgaccggca gtaaaacgcc gtcgtgcgtc
     5341 agtgcggtat aggcgcgcgc caattgcagc aggctcaatt gcaggccgta accgaaagac
     5401 atcgtcgcct gttcgatagg ccgccacctg cgccaatttc tcaacaaacc tgcagtttcg
     5461 cccggaaagc ccgagtgcat acgcacaccg atgcccaact catgatagaa gtcatacatt
     5521 tcttcggcac cgaaacgcgc agacagtttg cttgtgccga cgttggacga tttctgcatg
     5581 atgccgcgca catccaaaga ggggtaaaca tgggtatcgc gcacgggaga cggtccgatt
     5641 ttataaggct gcgtattcag ccgttcgttc aaatcggttt tgcccgcatc caatgccttc
     5701 gcaatcacaa acggtttgat tgccgaaccg ggttcgatca tatcggttac ggcacggttg
     5761 cgccgctgtt cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg cgtattggcc
     5821 aaggcgagga tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga
     5881 tggtattcga ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg
     5941 gaaaggatga tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc
     6001 acaatattgc cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg
     6061 tcttcaagcg aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg
     6121 acgtgtgcaa acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag
     6181 ttttccaaac ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc
     6241 cagataaacg acttgccttt ctgttcgagc ttgttcctca aaacatcaac cggcacatcg
     6301 acaagctcgg acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc
     6361 acggcaaaca gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa
     6421 accgtaccgc gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct
     6481 ttcaaaaagt tatacgttac cgtctgcaga tacagtccgc gagcaatcag accggcaaac
     6541 aagaccgcta ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg
     6601 acctgctctt ctttgggcag catccgaggc ttatattcgc tcttaatcaa catttttact
     6661 tctcgttatt attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt
     6721 tgatgctcca ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc
     6781 gccgccctga tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa
     6841 tcctgctcca aggcgatttc ctgttttttc gccttatcca aagctgtgaa attgagcctg
     6901 tactggtttt gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg
     6961 aaattcaatt tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcggac
     7021 cggcagtccg ctccgccaca cgcaaaaccg cacttctcgc cctcggattg gcggcaattt
     7081 ccgcctcacc cggctttaat gccctgccca cgattttcag gggcagctcg ggcaaatccg
     7141 cttccctgac cgccgcccag cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact
     7201 gcttcacaat gcgatcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt
     7261 tcagacgaca catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa
     7321 agatgcggac cgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga
     7381 cgttttgtgc cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt
     7441 gcgcaacaat ggcgcgcgca atccggcggc taaaccgctc ttcaccataa ttcttgatta
     7501 cctcgtgcaa atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgcg
     7561 tcgtatccat acgcatatcc aaaggggcat cgaaacggaa gctgaaaccg cggctgccgt
     7621 catcgatttg cggggacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac
     7681 ccaaaccgtc caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt
     7741 tgtccgaacg cgccagctct tctgccacag caatcgcctg cgggtctttg tcgaaaacaa
     7801 tcaaccgccc cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga
     7861 acgtaccgtc cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca
     7921 gcaagaccgt gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgact
     7981 caactggaag gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca
     8041 ctgctcgcga ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc
     8101 gaaatccacc ctcttcctca gtccggcaga aaccagcacc cggccggcgc tgtcccattc
     8161 caaaatttcc gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc
     8221 cacttttaag tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa
     8281 cagcttgtgt ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat
     8341 gtcacggaat ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc
     8401 gccgccgaac atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt
     8461 tgccccacaa ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag
     8521 ggcatgaaaa ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa
     8581 aaatgacgac aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata
     8641 tatgattaat ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac
     8701 ccgtaccaac gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca
     8761 tgcccctccc ctctcccgaa gcacggcaat tctcgctcaa actgcaaacc ctcattgccg
     8821 aaaaaatcgg caaacacggc aactggattc cattttcacg ttttatggaa ttggttttat
     8881 acgctccgca atacggctac tacaccggcg gcagccataa aatcggcaat accggggatt
     8941 ttattaccgc accgaccctc acctctctgt ttgcacagac actggcacgc caacttcaag
     9001 aacttctatc ccaaacggcg ggcaatatct atgaattcgg cgcgggaacc ggacagcttg
     9061 ccgccgattt gttgggcagc atttcggacg gcatcagccg ttactatatt attgaaatat
     9121 cgccggagct ggcagcacgt cagaaaaacc tgattcaagc acgcgcaccg gaagcatctc
     9181 aaaaagttgt ccacttgacc gcacttcccg aagcgtttga cggcatcatc atcggcaacg
     9241 aagtactcga tgccatgcct gtcgaaatcg tccgtaaaaa tgaaggcggc tcattcgagc
     9301 atgtcggcgt ttgcctagat aatgaccgtt ttacctattc ggcacgaccg ctgcacgact
     9361 tgcagctatc tgccttggct tccctctatt ttcctcaaac agattatccc tataccagcg
     9421 aactacatcc gcaacaatat gcctttatcc gcacccttgc ctcaagactc gaacacggct
     9481 gcatgatatt catcgactac ggttttgatg cagcgcagta ttaccaccct caacgcaatc
     9541 aaggtactct gatcggacac taccgacatc acattatcca caatcctttt gacttcatcg
     9601 gattggccga cctgaccgca catgtcaact ttaccgacat tgcacaagca gggacggatg
     9661 ccggattaga tttgataggt taccttcccc aatcccattt cttattgaac ttgggcatta
     9721 ccgagctatt ggcacagacg gggaaaacgg attcggcagc ctacatctgt gaagctgctg
     9781 ccgttcagaa actgattgac cagcatgaaa tgggcgaact gtttaaagtc atcgcattcg
     9841 gaaaaaatat cggcatcgac tgggcaggat tccgcttcgg cgacatctgc cacaaactct
     9901 aaccctcatg ccgcctgaat ccgcttcaga cggcataaac tttttaacat ttaaaaacag
     9961 tcaactaatt caaaattaaa aaatacggct tgtcaaaaaa acagaaaaac atataatagc
    10021 gtcttcacga aacggcgaat tagctcagtc ggttagagca gaggaatcat aatccttgtg
    10081 tccggggttc gagtccctga ttcgccacca aattttcggg ggtatagctc agttggtaga
    10141 gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg cttacctcca ccagataaaa
    10201 aagcacagac cgtaaaaagg tatgtgcttt tttattgcct gattgccagc aaataaagaa
    10261 taaaccactg ccttcaaaac aggcaatcga ctttaaacct atcgccccgc ctgtcctgat
    10321 tttatagtga attaaattta aaccggtaca gcgttggctc gccttgccgt actatctgcg
    10381 gcttcgtcgc cttgtcctga tttttgttaa ttcactatat cagcccgcca gacaaacccg
    10441 acccgaataa tgtcttcagg tcgggtttat ggtttcattc ccaacttatc cagcctgaca
    10501 gccacaatat aatgatggca ataccaaaaa caatgcggta ataggcaaaa ggaatataat
    10561 ttttcttgga aacaaacctc agcaacgctt ttaccgctac caagcctgaa acaaaggcag
    10621 caataaagcc tatcagaatc aaaccgacat catgcagggt gaaaaatcgg taatgtttca
    10681 ggacatcata agccgttgcg gcaaccatca tcggcacagc caagaaaaac gagaattctg
    10741 tcgcagtttt ccgttcgatg ccccaaagca tcccgcccat aatcgtactg cccgaacggg
    10801 acgtacccgg aaccagtgca aacacttggg caacgccgat catcaaggca tcaatcggac
    10861 gcaatgcatc aacatcggca attttaggct ctgctcggct ttggcgtttc tccacccaca
    10921 aaataaaaaa accgcccaaa accagcatga ctgcaacact caaggggtta aacagatact
    10981 ctttgatttg tttgccgaac aacagcccca tcacggcggc aggtataaaa gcaatggcaa
    11041 gattaaggac gaagcggttg gctttccggt cttttcccaa gccgtgcaac acattgctga
    11101 aacgttgccg gtattcaaac actaccgcca aaactgcacc gagctggatg gcaatttcaa
    11161 aaaccttgtg attgctgtga aaaccaatca gattgccgaa cacaatcaaa tgtccggtgc
    11221 tggaaatcgg taaaaattcg gtaaaacctt ctaccaagcc catcatcagg gctttcagga
    11281 caatcagaaa atccattgct tgcgcttctt tcggatacgg gagttcggct atttctgtac
    11341 agcaggggtc tgacgcttgc gttcttccct gactttggca accaattctt taatcgtatg
    11401 aacgccgccg tcaaagccgt tattgaagat aacgcggtat tttccgccga caataacggt
    11461 cggcgtgctg tcgatgcggt attgttccgt cagtttctgc atttttaatg cggcggcggc
    11521 agcttcgggg gaatcatagg cgcgcatcag ttttttgccg tcaaagcctt tttgagacaa
    11581 agcccatttt ccggcaaccg acctgttttc caagcggatt ttttgttcgt aaactgcttt
    11641 aaacacagca gggtttgcct gatatttcaa acccgacaaa ttgacggcag ccgccatcct
    11701 agccaaaccg agcatttcag gctgccagac cacgtgctcc gtcctcaaat aggcatcaga
    11761 cggcaatgcc ttgcccagtt tcaataacaa aggatcgaaa tgatggcaat gtacgcagaa
    11821 atagccgaaa aattccaaaa cctcaatttt acccgactgt tcttgaggaa tgggtttatc
    11881 caacacaaga tagtcttccc cttccgtcag ggcatatgcc tgcgcggaca acactgccga
    11941 cagcagcagc ggcaacagat gtttgagctt cataattatt tgctttcgat agaacggatc
    12001 aggctggcga cttcatgttt tttcaactcg tcctgcattt ttttcaccgc atcggcagac
    12061 atattgccgc tttgcacccg gtaaagcgtt ttatgtcccg cctgataacc gaccaccttg
    12121 gaagatatgc ccaagattgc cagtttggca cgctgccctt ccgcgctctg acggtcggca
    12181 tacgcgccca tttgcagata atgcgttgct tccgccttgt cggacgtttt cattttctgc
    12241 acttctttgg cggcggcact gcgcgctttt tcgatgctgc cgctgttgag gatttgttcc
    12301 ggggttggtt tgggtgcaac tttttccttc gccgcctttt tctcttcttt tgaagctttt
    12361 ttctctgttt ctttagacgg ttttaccgct tgttttttaa ccgtttcggc atctttcttc
    12421 tgcgcttttt ccctgacggt ttgttcacgc tcttccgtca gcgctttctt acgcactgcc
    12481 tgtccgtccg gctcttcccg ttccggctcg cccgcctttt cttcaacctc gtcggcttta
    12541 tcggcaacgg gctgcttgtc ggcagctttt tccgcatccg actgctctgc ctctgtcgca
    12601 gcatccggtt cggacaaggc gttttgatcg gccggttcag gttggatgtc ttccttaggc
    12661 tggtttttcg gtttcaggat ttccgtttct gcaggctgct tcgacgaagc cgggattttg
    12721 aacgcatttt gaccgctctg gttcagataa aacaaaatac cggcaataat gaccgtcgcc
    12781 agtatcaaac cgaagaaaaa accggacaga ccttttccgg attgggaaaa tttgttcata
    12841 aacatacctt aatgtgtttc agacggcatt aacgccgttt gcttacgggc ggatattcta
    12901 acaatatcgc catatttggg caaaacctgc ttccattccc attcctataa agcacgacgg
    12961 aaacctatgg ctttcatgac gtggcttctg ccgacttctt cgtcgcccgc caaatccgcc
    13021 aatgtacgcg ccacgcgcat aatgcggtgg aagctgcggg cggaaaggga gagtttttcc
    13081 agcaggccgc ccaatgcttc ctgcgcttct ttttgaatgc gggcggatgt gtcgagttca
    13141 ctgacactca aggcggcatt cactttgcct tgccgtgcgt attgtttgtc tctagcggcg
    13201 ataacgcgtt ccaaaacgga cgcgctgctt tcccctgctt cctgctgcat cagttcggcg
    13261 gcggacaggc tcgggacttc gatggtcaaa tcgatgcggt cgagcagcgg cccggaaatc
    13321 ttgctgcggt aacgcgcgac gctttcgggc gtgcagcggc agggtttgac gggatgcccg
    13381 agataaccgc acgggcaggg gttcatggcg gcaacaagtt ggaatttggc aggatagacg
    13441 gcttggcgcg ccgcgcggga aatgtggatt tcgccgtttt ccaacggttc gcgcaaaact
    13501 tccaaaactt tgcggtcaaa ctcgggcagc tcgtccaaaa acaaaacgcc gtggtgcgcc
    13561 aatgaaattt cgcccggacg cggatccgaa ccgccgccga ccatagccgc cgcgctggcg
    13621 ctgtgatgcg gactgcggaa aggacggttg ctgtcgagtt gttgttggtg gttgggcagg
    13681 agcgaacgca atgcccaaac ttctaccaat tcgtcttcgg tcagcggcgg caggatgccg
    13741 ggcagccgtt gggagagcat agacttgccc gttcccggcg gacccatcat caagaggctg
    13801 tgtccgcctg cggcagcgat ttccaaagca aggcgcgcgg tgtgctgacc tttcacatcg
    13861 cacaaatcag gttgtccgcc atgttcaaac ggcatctgag gaacttggca ttcggtttgc
    13921 gccaaaggtt cgatgccgtt caaatgggcg gcgacttcgc ccaaagagcg cgcgccgtaa
    13981 acggtaatgc cgcgcatcac ggcggcttgt cctgcatttt cttcaggcaa aacaaatgca
    14041 cgttttgcct gcataccctg ccacgccatc gccaacgcgc cacgcacggg gcgcaacagc
    14101 cccgacagtg ccaattcccc cgcaaactcg tattcctcca gtttttcggg cgcaacctgc
    14161 cccgatgcgg caaggatgcc gattgcaatc ggcaaatcga aacgccccga ctctttgggc
    14221 aggtcggcgg gggcgaggtt gacggtaatt tttttggcgg ggaattcaaa accgctttga
    14281 ataatggcgg cacggacacg gtcgcgactt tcctttactt ccatatcggg cagtccgacg
    14341 atgttgaaat gtggcaggcc gttggcaagg tgggcttcca cttcgaccaa cggcgcattc
    14401 ataccgctca aggcgcggct gtaaaccaag gcaagcgaca tatttcagac gaccttattc
    14461 gccggcttcg gtttgctgcc tgatttcggc gacggcttct tcggcagcgg cttcagccgc
    14521 ttccaatgct gcccgttcgg gattttgcgc ggcttcgagt ttttccaaac gcgcttccaa
    14581 agccgccagt ttggtacggg ttttgattaa aacctgctgc tggatgtcga attcttcgcg
    14641 cgtaaccaga tccatacggt tgaacgcgcc gcccagcatc gccttaatat ttttttccac
    14701 atctttggca gggctgttgg cgatggtttc gctgattttc gagccgactt cctcaaaaag
    14761 ctgcttgccg aacataatct gtatccttcc tgaacatatc aaattcaatc ggctattgta
    14821 taaggaaaaa tgccgtctga aaacgggcgg cggataatcg gcaaaacata ccgcgcctcc
    14881 tttgcggttg ctaaattaca cctcaaaaca cctgccgcct gaaaacggat ttcatattcc
    14941 gcgcaacgcc cgattacaag acactacaaa acaatatgct gttttaaatg atttttccga
    15001 cgcgcatcgt ttcaaactcg gctttttaag ccattaagtg ctttgcaaac aacagaaatt
    15061 gggttatcct gaaacggatt atttacaatt tcatatagtt ttattacata tcttattgtg
    15121 attgaagata atttatccga atcccccttt cgggtatccg gattttccgt tgtactttta
    15181 ttagaaaaac atttcaggcg caagttgctt gcaatttcaa agcctgtaat tctgaagttt
    15241 tagacgagga atagcacatg aatcccatgt acattacttt tgcaatctat ctggttgcag
    15301 tccttctcat cgggcttgcc gcctattttt ctacgcgcaa tttcgacgat tatattttgg
    15361 gcgggcgcag cctaggcccg tttgttaccg cgatgtcggc aggcgcgtcc gatatgtccg
    15421 gctggctttt gatgggtctg ccgggcgcga tttatttgag cggtttgaat gaggcttgga
    15481 ttgccatcgg cctcttggtc ggcgcgtatt tcaactggct tttggtggcg ggccgtctgc
    15541 gcgtacatac cgaatacgcc aacaacgcgc tgacgctgcc ggattatttc ttccaccgct
    15601 ttggcgcggg cggacacttg atgaaagtgg tttccgcact gattatcctg tttttcttca
    15661 cgatttattg cgcctcgggc attgtggcgg gcgcaaccct gttccaaagc ctgtttgaag
    15721 gtatgactta caatcaggca atgtggctgg gcgcgggcgc gaccatcgcc tataccttct
    15781 tgggcggctt tttggcggta agctggacgg atacgctgca ggcttctttg atgattttcg
    15841 cgctgatttt aacgcctgtg atggtctatc tgggcttggg cggcgcggaa cagatgtctg
    15901 ccgcgattca aagtgtcgcc gcaggcacgg gcaaagaata cggcagcctg tttgccggta
    15961 cgaccgtcat cggcatcatt tccaccgccg catggggctt gggctatttc ggacagccgc
    16021 acattttggc gcgctttatg gcggccgaaa gcgcgaaatc gctggtatcc gcacgccgca
    16081 tcggtatgac ttggatggcg ttgtgccttg cgggcgcggt agcggtcggt tatttcggca
    16141 ttgcgtattt cggtgcaaat cccgacaaag tttcttctat gagcggcaac cacgaacgca
    16201 tcttcatcgc gctttccacc ctgctgttca acccttggat tgccggcatt attttgagcg
    16261 cgattttggc ggcggtgatg tccaccctgt cctgccagct tttggtttgc tccagcgcga
    16321 ttaccgaaga cttctacaaa ggctttttgc gtaaaaacgc gcaacagtcg gaattggtat
    16381 gggtcggccg cctgatggtc ttggcgattg ccgtgatttc catcctgatt gcttccgatc
    16441 cgaacagcaa agtattggga ctggtgtctt acgcgtgggc aggtttcggc gcggcattcg
    16501 gcccgattgt gattctgtcc gtattgtgga aacgcatcac tgcctacggc gcgttgtcgg
    16561 gtatggttgc aggtgcgtta accgtagtcg tatgggcgga atgggtgaaa aaaccggctc
    16621 aagcggcagg agaaagcggc ttgttgacga tgtacgaaat cgtgccgggc tttattgttt
    16681 gcttgatcgt tgccgtgttg gtttccctgt tcaacaaaga accttcgcgc gaaacccaag
    16741 aacgctttga aaaagccgac gcagattacc gcgccacccg ataaacccct ttcagacggc
    16801 acggcatccc ggttcgtgcc gtctgaaccc gtttccgggt caaacacaaa taaaccttgc
    16861 ctgcatacag gccttatttg tgttttaccc gtttcatttt caaagaaacg gaagcaccta
    16921 ccctgcccgc atttgaagcc ttgtatttca tctgcggatt tattacgata ttaccgtatt
    16981 caggccgcac cgatgccgcc tgccccccga aaaactttgg agaatccaaa aaatgtttca
    17041 ttttgcattt ccggcacaaa ctgccctgcg ccaagcgata accgatgcct accgccgtaa
    17101 tgaaatcgaa gccgtacagg atatgttgca acgtgcacag atgagcgacg aagagcgcaa
    17161 cgccgcctcc gagcttgccc gccgtttggt tacccaagtc cgcgccggcc gcaccaaagc
    17221 cggcggcgtg gatgcgctga tgcacgagtt ttcactctcc agcgaagaag gcatcgcgct
    17281 gatgtgtctg gcagaagccc tgctgcgtat ccccgacaac gccacgcgcg accgcctgat
    17341 tgccgacaag atttcagacg gcaactggaa aagccatttg aacaacagcc cttccctctt
    17401 cgtcaatgct gccgcctggg gcctgctgat taccggcaaa ctgaccgcca caaacgacaa
    17461 acaaatgagt tccgcactca gccgcctgat cagcaaaggc ggcgcaccgc tcatccgcca
    17521 aggcgtaaat tacgccatgc ggcttctggg caaacagttc gtaaccggac agaccattga
    17581 agaagccctg caaaacggca aagaacgcga aaaaatgggc taccgcttct ccttcgatat
    17641 gttgggcgaa gccgcctaca cccaagccga tgccgaccgc tactaccgcg actatgtcga
    17701 agccatccac gccatcggca aagatgcggc aggacaaggc gtttacgaag gtaacggtat
    17761 ttccgtcaaa ctttccgcca tccatccgcg ctactcgcgc acccaacacg gccgcgtgat
    17821 gggcgaactg ttgccgcgcc tgaaagagct gttccttttg ggtaaaaaat acgatatcgg
    17881 tatcaacatc gatgccgaag aagccaaccg tctggagctg tctttggatt tgatggaggc
    17941 tttggtttca gaccctgact tggctggcta caaaggtatc ggtttcgttg tccaagccta
    18001 ccaaaaacgt tgtccgttcg ttatcgacta cctgatcgac cttgcccgcc gcaacaacca
    18061 aaaactaatg atccgcctcg tcaaaggcgc gtattgggac agcgaaatca aatgggcgca
    18121 agtggacggc ttgaacggct atccgaccta cacccgcaaa gtccacaccg acatctccta
    18181 cctcgcctgc gcgcgcaaac tgctttccgc gcaagacgcg gtattcccgc aatttgccac
    18241 ccacaacgcc tacactttgg gcgcaatcta ccaaatgggt aaaggcaaag attttgaaca
    18301 ccaatgcctg cacggtatgg gcgaaaccct gtacgaccaa gtcgtcggcc cgcaaaactt
    18361 aggccgccgc gtgcgcgtgt acgccccagt cggcacacac gaaaccctgc tcgcctactt
    18421 ggtgcgccgc ctgttggaaa acggcgcgaa ctcgtctttc gtcaaccaaa tcgtcgatga
    18481 aaacatcagc atcgacacgc tcatccgcag cccgttcgac accatcgccg aacaaggcat
    18541 ccacctgcac aacgccctgc cgctgccgcg cgatttgtac ggcaaatgcc gtctgaactc
    18601 gcaaggcgtg gacttgagca acgaaaacgt attgcagcag cttcaagaac agatgaacaa
    18661 agccgccgcg caagacttcc acgccgcatc catcgtcaac ggcaaagccc gcgatgtcgg
    18721 cgaagcgcaa ccgattaaaa accctgccga ccacgacgac atcgtcggca cagtcagctt
    18781 tgccgatgcc gcgcttgccc aagaagcggt tggcgcagcc gttgccgcgt tccccgaatg
    18841 gagtgcgaca cctgccgccg aacgcgccgc ctgcctgcgc cgttttgccg atttgctgga
    18901 gcagcacacc ccagcactga tgatgcttgc cgtgcgcgaa gcaggcaaaa cgctgaacaa
    18961 cgccattgcc gaagtgcgcg aagccgtcga tttctgccgc tactacgcaa acgaagccga
    19021 acataccctg cctcaagacg caaaagccgt cggcgcgatt gtcgccatca gcccgtggaa
    19081 cttcccgctc gccatcttta ccggcgaagt cgtttccgca ttggcggcag gcaacaccgt
    19141 catcgccaaa cccgccgaac aaaccagcct gattgccggt tatgccgttt ccctcatgca
    19201 cgaagccggc atcccgactt ccgccctgca actcgtcctc ggcgcaggcg acgtgggtgc
    19261 ggcattgacc aacgatgccc gcatcggcgg cgtgattttc accggctcga ccgaagtggc
    19321 gcgcctgatc aacaaagccc ttgccaaacg cggcgacaat cccgtcctga ttgccgaaac
    19381 cggcggacaa aacgccatga ttgtcgattc caccgcactt gccgagcaag tctgcgccga
    19441 cgtattgaac tccgccttcg acagcgcggg acaacgctgc tccgccctgc gcattttgtg
    19501 cgtccaagaa gacgttgccg accgtatgct cgacatgatc aaaggcgcta tggacgaact
    19561 cgtcgtcggc aaaccgattc agctcactac cgatgtcggc cccgtcatcg atgccgaagc
    19621 acagcaaaac ctgttgaacc acatcaacaa aatgaaaggt gttgccaagt cctaccacga
    19681 agtcaaaacc gccgccgatg tcgattccaa aaaatccacg ttcgttcgcc ccatcctgtt
    19741 tgaattgaac aacctcaacg aactgcaacg cgaagtcttc ggtcccgtcc tgcacgtcgt
    19801 ccgctaccgc gccgacgaac tcgacaacgt catcgaccaa atcaacagca aaggctacgc
    19861 cctgacccac ggcgtacaca gccgcatcga aggcacggta cgccacatcc gcagccgcat
    19921 cgaagccggc aacgtttacg tcaaccgcaa catcgtcggc gcagtcgtcg gcgtacagcc
    19981 cttcggcgga cacggtctgt ccggcacagg ccccaaagca ggcggttcgt tctacctgca
    20041 aaaactgacc cgcgccggcg aatgggttgc cccgaccctg agccaaatcg gacaggcgga
    20101 cgaagccgca ctcaaacgcc tcgaagcact ggttcacaaa ctaccgttca acgccgaaga
    20161 gaaaaaagcc gcagcggccg ctttgggaca cgcccgcatc cgcaccctgc gccgtgccga
    20221 aaccgtcctt accggaccga ccggcgagcg caacagcatc tcatggcacg cgcccaaacg
    20281 cgtttggata cacggcggca gcacggttca agcctttgcc gcactgaccg aacttgccgc
    20341 ctccggcata caggcagtgg tcgaacccga cagccccttg gcttcctaca ctgccgactt
    20401 ggaaggtctg ctgctggtca acggcaaacc cgaaaccgcc ggcatcagcc acgttgccgc
    20461 cctgtcgcct ttggacagcg cgcgcaaaca ggaacttgcc gcccacgacg gcgcactcat
    20521 ccgcatcctc ccttcggaaa acggactcga catcctgcaa gtgtttgaag aaatctcttg
    20581 cagcgtcaac accacagccg ccggcggcaa cgccagcctg atggcggtcg ccgactgatt
    20641 ttgccgaaat acccgggcgc ggcccgtgaa ccaatgccgt ctgaaaacct ttcagacggc
    20701 atttttata
//
LOCUS       DAWISC010000017        19917 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWISC010000017 DAWISC010000000
VERSION     DAWISC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.017"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WIW87_000438"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WIW87_000438"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ5173645.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCCLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3080..3868)
                     /locus_tag="WIW87_000439"
     CDS             complement(3080..3868)
                     /locus_tag="WIW87_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ5173646.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WIW87_000440"
     CDS             complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WIW87_000440"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ5173647.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WIW87_000441"
     CDS             complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WIW87_000441"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ5173648.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6762..8072)
                     /locus_tag="WIW87_000442"
     CDS             complement(6762..8072)
                     /locus_tag="WIW87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ5173649.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WIW87_000443"
     CDS             complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WIW87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ5173650.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WIW87_000444"
     CDS             complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WIW87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ5173651.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10838..11566)
                     /locus_tag="WIW87_000445"
     CDS             complement(10838..11566)
                     /locus_tag="WIW87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ5173652.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11556..12470)
                     /locus_tag="WIW87_000446"
     CDS             complement(11556..12470)
                     /locus_tag="WIW87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ5173653.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12583..13992)
                     /locus_tag="WIW87_000447"
     CDS             complement(12583..13992)
                     /locus_tag="WIW87_000447"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ5173654.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WIW87_000448"
     CDS             complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WIW87_000448"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ5173655.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15218..16543)
                     /locus_tag="WIW87_000449"
     CDS             complement(15218..16543)
                     /locus_tag="WIW87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ5173656.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16669..18006)
                     /locus_tag="WIW87_000450"
     CDS             complement(16669..18006)
                     /locus_tag="WIW87_000450"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ5173657.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18090..18686)
                     /locus_tag="WIW87_000451"
     CDS             complement(18090..18686)
                     /locus_tag="WIW87_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ5173658.1"
                     /translation="MKQTVKWLAAALIALGLNRAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRRDDAEAVRWYRQAAEQGLAQAQYNLGWMYANGRGVRQDDTEAVR
                     WYRQAAAQGVVQAQYNLGVIYAEGRGVRQDDVEAVRWFRQAAAQGVAQAQNNLGVMYA
                     ERRGVRQDRALAQEWFGKACQNGDQDGCDNDQRLKAGY"
     gene            complement(18812..19894)
                     /locus_tag="WIW87_000452"
     CDS             complement(18812..19894)
                     /locus_tag="WIW87_000452"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ5173659.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4735 a         5567 c         5011 g         4604 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcagc aggtaacggg aacttcaacg gtttcgccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt gcgttcgccg accacgtcgt aggtttccgt accgtccagt
      601 tgcagggtat ggcggttggt gtcgggtttg aactgcagcg gcaacacgcc catgccgata
      661 aggttggtgc ggtggatacg ctcgaagcct tcggcaacaa tcgcttctac gccggcgagg
      721 cgtacgcctt ttgcagccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tcgccttcgg gttcgacgcg ggcgaacgag ccttggcgca cgctgccgtc ttcgtttttc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagcgcg
     1141 ccttcccagt aaggcggacg gcggatgtag gtggacatcg gacgccaatc gtacagcgga
     1201 ctgggtgctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcggc aacgacggca tcgatttctt catcggcagg ccaaatgtct
     1321 ttcaggcgga tttccttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg
     1381 atactgcctg ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc
     1441 gcatacgggt ggatacggcc gtcgaagttg cggttgcctg ataatacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctggatt ttcggatcca gcgcgccact catgccgttg
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcaacagg
     1621 cccgcttctt tcaaatagat ttcggctact tttgaacccg gggcaaacga agatttcacc
     1681 caaggtttgc gtttcaagcc gagacggttg gcattgcgtg ccaagagcgc ggcggcaaca
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgagccg
     1801 tcgggcattt ggccgtccga aggctcttcg taaggcttcg ccagcccttt cgccgccaaa
     1861 tcggcggtcg caaaacgggc atgcgggtta cttgggcctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgaggataa acggcggttt tcaaggcatc tgcccacaag
     1981 cctgcggttt tggcgtaggt ttccaccaat ttcacctgcg cgtcgtcgcg tccggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactcc
     2101 ggcgtcatgt tggaaatggt cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg
     2161 aagaattcga caaacgcccc gaccacgcgt tctttgcgca gaaactcggt cagtgccaac
     2221 acaatatccg tcgccgtaat gcccgcctgc cgtttgccgt tcagctcaac gccgacaata
     2281 tcgggcaggc gcatcatgga cgcgcgtccc agcattacgg tttccgcttc caatccgccc
     2341 acgcccacgg aaatcacgcc caatgaatcg acgtgcggcg tatgtgagtc agtaccgacg
     2401 caggtatcgg ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaacgcggtt
     2521 tttgtccagt tgatgaagtg gaaacggtct tcgttacggc ggtcttcgat ttcgcggttt
     2581 ttgcggaagg catcaggatc gtaaccgccg cactccaccg ccagagagtg gtcgacgatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca
     2701 tcgcgcaggc ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accacggaaa gtcgatttcc tgcttccctt ctatcaactg ccccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctgtggat ctgtttttct tgtggtttga ggtttcagcc gacgttttga
     3061 aggggtcgtc tgaaagggtt taaaacatcg aaacaatcat ctgatacagc ggatttctct
     3121 cgtctatcaa caatttcaca gccatcgaaa tgctgatgac aatcagcagc ggcttaatca
     3181 gcttcgaacc gaagcggacg gcaaatctcg cacctaaatt cgcaccgaca aacgcaccga
     3241 ccgccatcgt tgccgcaatc gggaaaataa tcgaaccgtg cagcaggaat accgatagcg
     3301 aaccaagatt gcaggcaacg ttcgccaatt tggtgtaaga catcgcgttc aacagcttgc
     3361 agccgagcaa aacaataaag gcaatcagaa aaaacgagcc gacacccggt ccgaacacac
     3421 cgtcgtaaaa acccaaaagc ggtgcgaccg tcagcccgaa cagaaaaaaa gacattctgg
     3481 ctttgccttc cttactgccg tcgagcttgg gcgaaaacac aaaatacagt gcgacaaata
     3541 tcaacaaaac cggcacgacc gccagcagaa tatctttgga aaccaagctg accgataatg
     3601 caccggccac gccgcctaca aacgatgctg cggcaatcgg gagacctttc ttccaatcaa
     3661 tcaaaccttt gcgtgcaaaa gaaaccgtag ctgaaaacgt agcagcggct gcttgcagct
     3721 tgttggtggc aattgccgac acgggaggaa tacctgccaa caagagtgcg ggcagcgtaa
     3781 tcaaaccacc cccgcccgca atcgcatcga taaatccggc aatcatcgca accaaaccca
     3841 aagcgagtat tatatataaa tcttccatgt ttcttatcct gttacttgcg ccaatacagg
     3901 attatcttct cctattagat taaacttatt tcagacaacc tttccaataa ggcaaggtcg
     3961 tctgaaatcc ttagctttgc ataccgaaat taaagaacaa actgctattg cattcttcaa
     4021 ttatcgttct tcaatctcca caaacgccaa atcttcaggg cctgtgtagt ttgcgctcgg
     4081 acggatgatt ttgccgtctt tgcgttgctc aagaacgtgt gcgctccaac cggttgtacg
     4141 ggaaattacg aacagcggtg tgaacatagc ggtcggtacg cccaattttt ggtaggaaac
     4201 ggcagagaac cagtccagat tcgggaacat ttttttctct tcccacatca cgctttccaa
     4261 acgttcggca atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac cgcgtgccac
     4321 ttctttaatg acaacgttgc gagggtcgga aatggtgtac accggatgac cgaaaccgat
     4381 cacgatttct ttgcggccga tgcgttcgcg gatgtcggct tcagcttcgt cggcattgcg
     4441 gtagcgtttt tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg gacctttcaa
     4501 cgcgccgatt gctccggtaa tgctggagta catatcagag cctgtaccgg cgatcacgcg
     4561 ggcggtaaag gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg aaacgtgcat
     4621 ggctttgatg tgtgattcgc ttgggcgttt gccgtgcaac agttgcagga aatgaccgcc
     4681 gatggtctct tcgtcgcttt caacctcaat gcgtttgccg ttgtgcgaat attgatacca
     4741 gtacaagagg atgctgccga ggctggcgat cagtttgtcg gcgatgtcgc gcgcttcact
     4801 ttccggatgg ctttcacgtt caggatgaac gcagcccagc atggatacgc cggtacgcat
     4861 tacgtccatc ggatgggtat gtgcaggcag gctttccaaa actttaatca cacggatagg
     4921 caggccgcgc atggatttga gcttggtttt ataagcggcc agctcgaatt tgttgggcag
     4981 atggccgtga atcagcaggt gggcgacttc ttcaaactcg catttttgtg ccaaatccag
     5041 aatgtcgtaa ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac acaaagcggt
     5101 attaccggcc gcaacgccag aaagcgcaac ggatttttta ggtttgaggg tcggggtttg
     5161 agtagtttca gtcatggtat ttctcctttg tgtttttatg ggtttcgggt tttcagacga
     5221 ccgatgcgga tttgttgaaa ggcagtctga aagcggtaaa tcatttttga aacaatttat
     5281 ccagtttttg ctcgaaggca tgatagttca gatgctcgta cagctcggca cgggtttgca
     5341 tactgtccac caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa acattcagag
     5401 cggctttgct tgctgcacgg aacgatgaca gcggatacag caccagcgac acgccgtttt
     5461 cagccagctc gctttgggta taaagcggag tggaaccaaa ctcggtaatg ttcgccaaca
     5521 cgggcacttt caccgcatct gcaaattggc ggtacatgtt caaatcggtc atggcttcag
     5581 ggaaaatcat gtccgcaccg gcttcgacac aagcttgggc gcgttcgata gcggcatcca
     5641 aaccttctac cgccagcgca tcggtacgcg ccataatcac gaagttctca tcaacgcgcg
     5701 catctacggc agctttgata cggtcgacca tttcatcttt agatacaatg gctttgttcg
     5761 gacggtggcc gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct gcaacaccgg
     5821 cgcgttcaaa gttgcgaatg gtacgggcaa tattgaatgc accgccccaa cccacatcga
     5881 tgtccaccag cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg atcagcacat
     5941 cttccattgt ggtaatgccc aaatcaggga taccgcaaga acaggctgcc acgccgccgc
     6001 cggacagata gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa taagcattga
     6061 cgcaaccggc gacggcaagc ggattcgatt ctttcacggc ttggcggaaa cgtgctccgg
     6121 cagagtgttg actcatcata tttctccttt atagactttt tttcagtatg gacaggcttc
     6181 catcacattc ggacggcaaa acacagccat ccgacgcgtc gggcatccca atccaatcat
     6241 taaaaatata tgggaaaaat tatcttattg atatttaaaa cgaatcaaag aaaaacagca
     6301 gaccgttcgg aattatgcgg caaaaccgca gacaagaaga aaacaagggg attattcaga
     6361 aaaggggaaa acatctgaat tggtttcata gtaatgttcc tttgtagtgt tatgtagttt
     6421 tatttttcga taatctaaaa cacaagactg aaaatgtcaa acatttttaa ggagactcat
     6481 tacataattt taatatttta ttttaattcc atgattaaac tagattaatt ttgaattatt
     6541 tgtttcaata aaattgtcag gaaatcaaga aaaatttttg ataatcaaaa tcctgaaaac
     6601 ttataaaata ttttatacat aaaaacaatg tattattttt aagcaatccg atgtatttta
     6661 ttatgtattt gatttttata taatttaata aaaattaaat atatcggcaa acaaaatgcc
     6721 gtctgaaaac ctttcagacg gcatttttaa tatcgaatca atcagcccaa ataccagaat
     6781 ttcagtgccc acaatacggc aacaatccat accataggcg gaacgtcttt ggtgcggcgg
     6841 cataaaagtt taaccacggc ataactgatg aagccgaaag cgatgccgtc tgcaatcgaa
     6901 taagtaaacg gcatgaaaac aatggtcagg aacgcaggtg cggcttccgt catatcgtcc
     6961 caatcaatat ccctcgcact gcggagcatc tgcgtgccga cataaagcag ggcgggcgcg
     7021 gtggcaaaag cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc gagcatcaat
     7081 acgccgacgg taaccgccgt caggccggtc cgtccgcctg ccgatacgcc cgccgcgctt
     7141 tccacataag gcgtggtgga agaagtaccc aaagccgcac ctgccacaat ggcggtagag
     7201 tctgcaagca gtgcgcgttt caggcggggc agcttaccgt ccaccagcag cccggcacgg
     7261 tgggatatgc cgaccagcgt tccggtactg tcaaatagat cgaccaagaa gaagacgaaa
     7321 atcacactga ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat aaaagtcggc
     7381 gcaatgctcg gtacttcgcc gatgatgccg tgaaattcat tcaaacccat caggctggca
     7441 atgacggtaa tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa atgtcccaat
     7501 acgaccacca tagcaaaacc gaacaatgcc aacaacgcgg acggctgatg aatatcgccc
     7561 aaaccgacca aggttgccgg attggcaacg ataatgcctg cgcctttcag ggaaatcagt
     7621 gccaaaaaca aaccgatacc ggcagcaatc gacattttca aacccatagg cagtgcgttg
     7681 accagcattt ccctgacttt aaaaaagctg aacaggataa aaatcagacc ggagatgaac
     7741 accgcaccca acgcaacctg ccaaggcacg cccataccct taacgacggc aaaggtgaaa
     7801 taggcattca gccccatccc cggtgcgagt gcaatcggat agttgccgac aaaacccata
     7861 acaaaacagc cgatggcaga cgcgatacag gtagcgacga ataccgcccc catatccatg
     7921 ccggtctcgc ccaaaatcag agggttgacg ataacgatgt agcacatcgt caaaaaagtc
     7981 gtcaaacccg ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag cttaaaaatc
     8041 ccgtccaaca gtgtttgttt tgaagtgtcc atatctgaaa gtctgtatca agtggaaaaa
     8101 tctgaatccg ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac atacctccca
     8161 aaccggacaa accgattcgg gaggcagcag gtttacgggc aaacagcaca ttatttgtct
     8221 gaattgtgtt gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac ggactgattg
     8281 tcgaaatccg cagcggtaag gttcatcgtg cggataccgc gattggtgcg gatcatacct
     8341 tcgacattgt ggctttgctc ctgtttggac ggagcaaccg cttctacctc ccttgccgga
     8401 acaaaatcga ccgcgccttt ttctttcaga ccggtagcga taatggtaat ccggatggca
     8461 tcttcgctca tggtctcgtc ttcagccgca ccgaatttgc attccaaatc gggatgcgcg
     8521 ctttggttga cgattttcat gacttcggac aactcggaca ttttcaagca acccggagca
     8581 gtcgtaatat tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc cagcagcgga
     8641 ctggaaatgg cctggtcggt cgccatacgc gcacggtcga taccttgggc ataacccgaa
     8701 cccatcatag cgataccgcg gttgctcatc acggttttca cgtcggcaaa gtcgaggttg
     8761 atgatttcgc tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc gcgcaataca
     8821 ttgtcggcgg cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc agtcatcagt
     8881 ttgtcgttcg ggatgataat cagcgaatcg acgtgttctt tcaactgttc caaccctgcc
     8941 tgtgcgacat ggacgcgctt accttcatat gcgaacggtc gggtaaccac ggcaacggtc
     9001 agaatgccca aagacttggc aatctcagca acaaccggcg cggaaccggt accggtaccg
     9061 ccgcccatac cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc ttcaatggct
     9121 tcccggtctt cctgggctgc cgcacggccg atatcgggat tcgcgcccgc gcccaaaccg
     9181 cgtgtcagat tcgtacccaa ctggattctc ttcgccgcat ggttttttgc cagagactgc
     9241 gcatccgtat tggcactgat aaactccaca ccgcgcacat tgttggcaac catgttattg
     9301 attgcattgc aaccgccgcc gcccaagccg attactttaa tcaccgcagg gctgactgcc
     9361 gattctgcca cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc attcagagga
     9421 cggtttaaat aaattattat tcattatata agaagtatct tgctgctggc aaaatacttc
     9481 tcacctgtca aacggcaatc cacctgttca gaagctgttt tcaatccacc gtttcaatct
     9541 tgccaacaaa ccgccgcccc cttccctctc ttgcactgca ccgttttccg gctgcggcaa
     9601 gtttccttcc agcttgcatg ctgcatgaag cagcccgata gcggtagaaa aacgcggtgt
     9661 gcggacgcgg tcggacaaac cgcccatttc ttggggtgca ccggtgcgta caggcaaatc
     9721 gaagattttt tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc cggtcagaac
     9781 gatacccgca ttcagcactt ctttggggaa acccgatttt tgcagctcgc ccagcactac
     9841 gccaaaaatc tcctgaatcc gtgcactgat gattgctgcc agaaccttac tggaaacctg
     9901 acgcgatgtc cggtcaccca cgcccggaac ttcaatcatc tcacccaagc cttccgtatc
     9961 gcatgatgcc acgccataat ggattttaat gtactcggcg gcatcgagag gtgttctcaa
    10021 cgatttggac aaatctttgg taatcagatt accaccggcc ggaatgacgg acgtatggcg
    10081 gatggcaccg ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt cgatgacgca
    10141 tacgccgagg tctttttcat cttcagtcag caccgcctgc ccgcttgcca acggctgaag
    10201 catgatctga tcgcttttca aaccgcaccg ctcgatacat ttttggacat tctgcactgc
    10261 cgtacttgca ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc tcataccgat
    10321 gggctccctc acgccaagtt gggtgtcaat aatgtagtct tgaaccacgg catcgagaat
    10381 tttttgatcg ggcgggatat tgattgcctt tgccgtttca atggcgcgat cgatgtctgc
    10441 ctgcgtgact tccccatctt taattttaac cacaccttgc gaattgagac tgcggatgtg
    10501 gttgcctgcg atacctgtgg taacgtgagt aattttggta tccgccatca gctcggcatc
    10561 attgaccgcc tgcctgatgg cttggacggt ggcatcgata ttggttacca tgcccgcgcg
    10621 caagccccgt gaaggagcct gccccaaacc gacgatgttg attttgtcgt catcttgaac
    10681 ttccccgatc agtgcgagga ctttagacgt accgatatcc agtacgctga tgtatctttg
    10741 ctgctgttcc attgttcgtc tgctcttaaa actgattgaa atttgcgtcg caccgtttca
    10801 gacggcacgg ccgtaatctg tccgatacct gttcccacta ttcttcggat tctttttcgg
    10861 gtaaaccgtc ggaagcatag cggactgaaa atccgtcctt atacctcata tccacatagg
    10921 ataaccgatt tttattttta cgcaacagat gctgccacgc ttcggtaaaa agccggaggc
    10981 gtttcatctc gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa acgacaatcc
    11041 acgccgaacg tgccgtatag gtcatctctt tgatgcccaa accctgtttt gccaaaacag
    11101 tcgaaaattc gtcataacgg cggagcattt cggcagacgt tccttccgcg cctctgaata
    11161 ccggcattcc gggtctgtcc aagcgggctt caaaaacatt gccttcgccg tccaccaagg
    11221 catggtcgcc ccaacgcgcg accggcttgc gctcggtcag gacgacctca accgtgtcgg
    11281 gaaaacggcg gcgcaccatg accgacgcaa tccacggata ccggcggtag gcctcctgtg
    11341 cgccattgat gtccgtcctc aaaatattcc catggatgta ttctttcgcc aaactgccca
    11401 atgtcttctt atcggaataa accaggttgc ccttcagcga cacctgcttg acgggcagat
    11461 gattcgaatt gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc atcatgacaa
    11521 gcagccagcg cgtcagccgt tccatcgctt cggcattatc ccacatgtgc ggtcttcaaa
    11581 atttcaatac ataaatcggc aaaacccacg cccgtaacgg cagcggattt cggtactaaa
    11641 ctatggctcg tcataccggg cagggtgttg atttccaaca gatagagttt gccgtcggta
    11701 tctttgagga aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc gccgcgaacc
    11761 gccagttcgc gcatcaggct ttcttcggct tcggtcaaat cttccgaagg acattgataa
    11821 atggtgtcgt cgcggttgta cttggcttcg tagtcgtaaa actcggttgc gggaatgatg
    11881 tgtatgccgg gcagcccttt gccgttcagg acggggcagg aatattcgcc gccgccgata
    11941 aaacgttcgg caatgatttc gccctgaagg tgtttcaatt cttcgtaaac gcttttcaga
    12001 cggccttttc ctttgacttt taccacgcct acgctgctgc cttcggccgc cggtttcaca
    12061 aacatcggca ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc gtgcaggacg
    12121 gcgaactcgg gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg gtatttgtcc
    12181 atgccgatgg cggatgcggc gacaccgctg ccggtatagg gaatgcccaa cagttccaat
    12241 gcaccctgaa ccgccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa tgccgtctga
    12301 aaaccttgtg ccttcaattc agacaatggg gtttctttag gatcgaaggc gtatgcgtct
    12361 atgcctttgc tttttaaagc attcaaaatg gcggtgccgc tgtccagcga gatttctcgt
    12421 tcgctggaaa aaccgcccat caatacggcc actttgccaa aattctgcat tgtttttgtt
    12481 ctttcctgat tgctttatgc ttgttgccag aggtcgtctg aaacctgatt tgcggtttca
    12541 gacgatcttt atatgatgtt ccgtctgtca ggcgggtgtg cctcaaatct gtttcgacaa
    12601 tgccagcagc gcggcgggga cgcggttgat gcttcccgcg cccatattca acacgatgtc
    12661 gccgtcctgc aaaacgttca acagcatttc gggcagatcg gcaacgtttt cgcagtaaat
    12721 cggctcgagt ttgcccaaca cgcggatggc gcgggcaaga gcgcgggaat cggcggcggc
    12781 aatcggctct tcaccggcgg cataaacttc ggtcagcacc agcgcgtcaa cggtattgag
    12841 gactttggta aagtcttcaa acaaatcgcg cgtgcgggta tagcggtgcg gctggaaggc
    12901 gagtaccaaa cgtttttcca gatacgcgcc gcgtgcggcg gcaagggtcg ccgccatttc
    12961 gacggggtgg tgtccgtagt cgtccaccaa gagcgcggtc ccgccgtttg gcaacttgat
    13021 gtcgccgtat ttttggaagc ggcggccgac gccttcaaag ccgagcaagc ctttttggat
    13081 cgcttcaacc gatgcgccga cttccagcgc cacgccgatg gctgccaatg cgttcagcac
    13141 gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac ccctgctcat gtcctttcat
    13201 ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt tcgatgtcgg tggcgtagat
    13261 gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg ctcactttgg gcaaaatcgc
    13321 gcggacgtgt tcgctgtcaa tacacaaaaa ggctttgccg tagaagggca tacggtggat
    13381 gaaatcgata aacgcctgat gcagtttttc gacgctgtgc ccgtaggtat ccatatggtc
    13441 ttcgtcgata ttggtaacga cggacataat cggtgtcagg tgcagaaagg atgcatccga
    13501 ctcgtcggct tcggcaacga tgtattcgcc tttgcccaag cgggcgttgg tgcctgcggc
    13561 gttgagtttg ccgccgataa cgaaagtcgg gtcaagtcct gccgcgccga ggatggaggc
    13621 ggtcaggctg gtggtcgtgg ttttgccgtg cgtgccggca atggcgatgc cgtcacggaa
    13681 gcgcatcaac tccgccaaca tcagggcgcg cggaataacg ggaatttgct gctccaacgc
    13741 agcgacaact tcgggatttt cttttttgac ggcggtagag gtaacgacga catccgcacc
    13801 gttaacgtgt tcggcggtat ggccgggata aacttgaatg cccaggctgc ccaaatgctc
    13861 ggtagcggca tttcgcgcct gatccgaacc ggaaacttta aagcccaaat tgtgcaagac
    13921 ttcggcgatg ccgctcatgc cgacgccgcc gataccgaca aaatggatgt tggtaactcg
    13981 atttttcatc ataatgttgc gttccggtgg attttcgatg cgtaaaggcg ttattttaaa
    14041 gggctgaccg tttgcgcgcc atagttttct gacaaatata tagcggattg aaataaaaaa
    14101 catccatgcc gtctgaacgg cttttcagac ggcatggttc ggcagtttac gccgcacacg
    14161 caatcgcggc ttccgccacg tcgtccgcac tgtgcggcag tgccaacgta cgggcgtttt
    14221 ctgcccattt gaggcatttt tcgcggttta agccgccgag aatctcggcg agtttttccg
    14281 ccgtcaactg ggtttgcggc aacagcaatc ccgcctccgc ctgcaccata aaacgcgcgt
    14341 tggcggtttg gtgatcgtca accgcgtgag gatacggcac taacaacgca cccaatcccg
    14401 ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca aatcaccaaa tcggcatcgc
    14461 ggtaggcgga caccatgtcg gtaataaatt ccacgcattc ggctttcacg cccagcgcgt
    14521 cgtaatccgc ctgcaagctg cccagcttgc cccgtcccga ttggtggtac atctgcggac
    14581 gcgcattgtc gggcagcaaa gccaatgcct gcggtacggt tttgttcaaa acgtccgcgc
    14641 ccaaactgcc gccgaccacc aaaattttca gacggccttc acgcccttgg aagcgttcgg
    14701 caggcacggg caggttgcta atatcggcgc ggacggggtt gccgaccaag ccgccttcgt
    14761 ggctgaacgc tttcggaaaa gcgtacaaca cccgcttcgc ccagcgcgac aggtggcggt
    14821 tggacaaacc tgccacggcg ttttgctcgt gaatcacaat cggcacgcct aatagcttcg
    14881 ccgccaaacc gccggggaag gtaacgaagc cgccgaagcc gatgacgcac tcgacacggt
    14941 gtttgcggat aatccgctgc gcttcgcgga cggtttgata caaagtaacc ggcagcatca
    15001 gtttgcgttt gatgccgttg ccgcgcacgc ctttaatcgc cagcgtttcc aagcgtatgc
    15061 cgtattgcgg cacgatacgc tcttccatcg aatccttgct gcccagccaa atcacatgat
    15121 ggccgcgcgc gcgcaatgaa tccgccaccg ccagcgcggg gaaaatatgt ccgcccgttc
    15181 cgcccgccat cagcataaag gttttaccgc ccatgattta ctccacccga taaccgcgca
    15241 ttttccggcg gttttcataa tctatacgca acagcagcat catgctgatc agcatgaaaa
    15301 agactgacga accgccatag gacatcaacg gcagcgtcag acctttggtc ggcaaagcac
    15361 cgatgttcac accgatattg aagaaacttt ggataccgat ccaaatgccg atacccgaag
    15421 cgatataggc gttgaaagtc aaacccaaat cgcgcgactg cttgccgatg gaaaacgccc
    15481 gcaccaccag ccagccgtaa cagaatatca gcacgcacat accgaagaaa ccgaattctt
    15541 cggcgatgat ggcaaaaata aaatcggtat gcgcttccgg cagaaagccg cgtttgctca
    15601 aactcgcacc caaacccata ccgaaccact ctccgcgccc gattgccatc agagagtggg
    15661 taagctggta gccggcaccc tgcgggtctt tccacgggtc caaaaatgcc actacccgct
    15721 gcacacggta gggagcggcg gtaatcatca gcaccatccc gcccaagacg ctgcctacca
    15781 ggacgaaaaa atatttccac ggcaatcctg ccaaaaacag cattccaacg gcaatgacgg
    15841 taatgacgac aaacgaaccg aaatccggct gtaccattat cagcaccaaa ccgaacgcca
    15901 ccagcataat cggcaggatg atcgcccgga aacggccgta catttctaat gtttcacgac
    15961 gtgcctgcgg attggtggcg gacatgatca gattggccgt cccccgccaa atcgactgcc
    16021 aacccaaact ttccatgctg cgcaacactt cttcacggcg cgtgaacagg cttgccaaat
    16081 aaaggatgac cgccagcttg aacagctcgg tcggctggaa attcaacgga cccaaaggta
    16141 tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa tacgactacc agcaacaggc
    16201 cggatagggc aaaaatccac ggcacaagcc gccgccatgt cctcatcctg caaagaaacc
    16261 ataacaaacc gctcgctatc aagccggcaa cgacgaaccc cgcctgtctg gtcaaataga
    16321 aaaactgatc gccgccttct tttgatgcca aatacacaga agccgaataa atcatcagca
    16381 ggctgaacgc cgtcatcagc accaccatcc acaaaagcgg cgcgtcgaat ttcctgccgt
    16441 cgcgcacaat cggcctgtcg agcagcagag tgtggacacc gtcgcccact tttaccaata
    16501 cttccgaaat cttcaaaaaa accacctgcc agtctgtttg cacctgccgc aaagggcaaa
    16561 aatttcagac ggcagacaat gccgtccgaa catacgatac atcccaaatc ggtattctaa
    16621 atctttactt gccgcccaac aatgacggcg tttgcatttc agacggcatc acaaagcctt
    16681 aaacgcttcg ataaacactt ccgaacggtg cgcgtagcct ttgaacatat caaagctcgc
    16741 gcaggcgggg ctgagcaaca caatatcgcc tgcttcggct tgggcatatg ccgtctgaac
    16801 ggcttctccc aaagtggcgc agtcggtcat attcaagccg cagccgtcca aatcgcggcg
    16861 gatttgcggc gcatcgacac caatcaagaa cacgcctttt gccttgccta ccagtgcatc
    16921 gcgcaggggc gtgaagtcct gccctttacc catgccgccc aaaatcacga agagcggatt
    16981 ttgcaaaccg gcaatcgcgg cggcagtcgc gccgacattc gtgcctttgc tgtcgtcgat
    17041 aaacaccacg ccgtttttct cgccgatttt ttccacgcgg tgcggcaggc cttggaaggt
    17101 tttgacgtgt tcgagcaatg cttcgcgcga caaaccgatg gcctcacaca aagccacggc
    17161 agccatgacg ttagcggcgt tgtgcagacc ttgcaacgga atgtcttgcg tgacaatcaa
    17221 atcttcattg ccttgtttca ggcggcctgt ctcgcgttcc aaccagaaat cagcttcgtg
    17281 ttccaacgaa aaccatttta cctcgcgccc ggcacgcttc atcgcgcggc agaacgcatc
    17341 gtccgcattc aaaacctgca cgccgtcgcc acggaaaatc ttggctttgg tatgcgcata
    17401 gtcgagcaag tcgtcgtagc ggtcgagatg gtcttcggaa atgttcagca ccgtcgccgc
    17461 agtcggacgc aggctttcgg tgttttccag ttggaagctg gaaagctcca acacccacac
    17521 gtccgccttt ttgccttcgc gctgccattc cgcctccaaa accggcgtgc cgatattgcc
    17581 cgcgataacg gtatccagcc cgcacttgat acagagatag ccgaccaggc tcgttaccgt
    17641 ggttttgccg ttgctgccgg taatcgcaat taccttgtcg tcccggcggt tcacaatgtc
    17701 cgccagcaat tcgatgtcgc ccaacacgcg tccgccgttt tgcttgaacg cctcaatatc
    17761 cggctgccgc tcgctgatgc cgggactgag agccagaata tcgaaaccgt tgtccagcgc
    17821 atctttcaga cggcccgtgt aaaacaccaa cccgtcaaac atcttaccga tttgcgacac
    17881 gcgttccggc ttcagctccg catcatacgc agcaacctcc gcgccgtttt tgcgcaggta
    17941 ggcaatcatg gaaatacccg taccgccgag tccggcgacg aggatttttt tgttttgaaa
    18001 agtcattttg gtttgtccta aaacaaatca tattgagcag gagatgtccg cccctgccca
    18061 agccgctttc agacggcatc gcgagctgtt caataacccg ccttcaggcg ttggtcattg
    18121 tcgcagccgt cttggtctcc gttttgacaa gccttgccaa accattcttg tgcaagggcg
    18181 cggtcttggc gcacgccgcg tctttcggca tacatcacgc ccaaattgtt ttgggcttgg
    18241 gctaccccct gcgctgccgc ctgccgaaac catctgaccg cttcgacatc gtcttggcgc
    18301 actccacgtc cttcggcata tatcacgccc aaattgtatt gggcttggac aaccccctgc
    18361 gctgccgcct gccgatacca tctgaccgct tcggtatcat cttggcgcac gccgcgcccg
    18421 ttggcataca tccagcccaa attgtattgg gcttgggcta acccctgttc cgccgcctgc
    18481 cgataccatc tgaccgcttc agcatcatcc cggcgcacgc cgcgtccttt gtaatacatt
    18541 gcgcccaaat tgtattgggc tgctgcattt ccctgtgctg ccgcctgcaa gttttcccga
    18601 aaatccgata cgtcatccgc ccacaccgct cggttcaagc ccaaggcaat cagggcggcg
    18661 gcaagccatt tgactgtctg tttcatggtt ttacttctgt tttagtataa ggcgggtttc
    18721 agccaccgtt aacgataggg ctgggcggat tgtcgccgca ggtttattgc gcgttcaaat
    18781 gccgtctgaa agatgttcag acggcatagg ttcagcggat tttgagggta ctcaaaccga
    18841 tcaacaccaa gacgatggta ataatccaaa agcggacgac gacttgggtt tctttccagc
    18901 ctttttgttc gtagtggtga tggatgggcg ccatcaggaa gatgcgtttt ttggttttct
    18961 tataccagcc aacctgaagc ataacggata cggcttctac gacaaataat ccgcccataa
    19021 tgacgaggac aaactcttgg cggacgataa cggcgacggt accgagcgcg gcacccaatg
    19081 ccaatgcacc gacatcgccc ataaagactt gcgcgggata ggcgttaaac cacaagaaac
    19141 cgaggcacgc gccgcacatg gcggtacaga aaatcaccac ttcgtttgcg ccggcaacgt
    19201 aaggtaattg caggtattgg gcaaattgtg agtggccgct ggcataggcg aagatggcga
    19261 ggccggcggc aacgaggacg acggggaagg tcgcaaggcc gtccaagccg tcggtgaggt
    19321 tgacggcatt ggatgtgccg acgatggtca ggtaagacaa caccaaaaag ccgaccacgc
    19381 ccagcggcag ggcgatttgt ttgaagaacg ggacaatcaa aatattgttg gcggaattgg
    19441 cggcaaggta aaacaatgcc aaactggcga taatggcaac gcttgactgc cacaccattt
    19501 tgaatttggc ggacacgccg ttggggtctt tatagacgac tttgcgccag tcgtcgtaaa
    19561 aaccgagtgc gcccgtggcg agcaatacgc ccaagagaat ccagatatac gggtttgccc
    19621 agttgcccca caacagggtg gacacggtaa tggcggtcag aatcagcgaa ccgcccatcg
    19681 tcggcgtgcc gtttttgacg aggtgggttt gcggaccgtc ggtacgcact gcctgcccgc
    19741 atttgagcgc ggtcagcctg cgtatcgtcc acgggccgaa catcagggaa aacgctaagg
    19801 cggtcaacgc cgccatgacg gcgcggaatg tggtgtattg aaaaatattc agaccggtta
    19861 accagttgct gaaatgtgcg agccataaaa acatggggct tccttttttt gttttgt
//
LOCUS       DAWISC010000018        19792 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWISC010000018 DAWISC010000000
VERSION     DAWISC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.018"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(203..278)
                     /locus_tag="WIW87_000453"
     tRNA            complement(203..278)
                     /locus_tag="WIW87_000453"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WIW87_000454"
     CDS             complement(371..730)
                     /locus_tag="WIW87_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ5173660.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WIW87_000455"
     CDS             complement(753..1397)
                     /locus_tag="WIW87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ5173661.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WIW87_000456"
     CDS             complement(1399..1935)
                     /locus_tag="WIW87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ5173662.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WIW87_000457"
     CDS             complement(1947..3044)
                     /locus_tag="WIW87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ5173663.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WIW87_000458"
     CDS             complement(3047..4483)
                     /locus_tag="WIW87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ5173664.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLILSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIW87_000459"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIW87_000459"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ5173665.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WIW87_000460"
     CDS             complement(5473..6120)
                     /locus_tag="WIW87_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ5173666.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCT
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFGEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIW87_000461"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIW87_000461"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ5173667.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPSTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            complement(7294..7431)
                     /locus_tag="WIW87_000462"
     CDS             complement(7294..7431)
                     /locus_tag="WIW87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ5173668.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7495..8571
                     /locus_tag="WIW87_000463"
     CDS             7495..8571
                     /locus_tag="WIW87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ5173669.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTIIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WIW87_000464"
     CDS             complement(8778..10244)
                     /locus_tag="WIW87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ5173670.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIW87_000465"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIW87_000465"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ5173671.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIW87_000466"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIW87_000466"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ5173672.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTETVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGNRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WIW87_000467"
     CDS             complement(13523..13807)
                     /locus_tag="WIW87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5173673.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WIW87_000468"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WIW87_000468"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ5173674.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAEFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVSNLSNGEDSGLQSPIVKYLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKATETKEV"
     gene            complement(15824..16525)
                     /locus_tag="WIW87_000469"
     CDS             complement(15824..16525)
                     /locus_tag="WIW87_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ5173675.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQPGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="WIW87_000470"
     CDS             complement(16668..17816)
                     /locus_tag="WIW87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ5173676.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WIW87_000471"
     CDS             complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WIW87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ5173677.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18543..19685
                     /locus_tag="WIW87_000472"
     CDS             18543..19685
                     /locus_tag="WIW87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5173678.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5086 a         5104 c         4613 g         4989 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caagtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ctgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaagaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat accgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatctg acggcgtatc aaagtctagt attaccgtaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agcctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgttt tcaagcctgt tttgcaatgg gtgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacattg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg tcattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgacg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgga taagatctgt
     5641 acgtctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgccaaa
     5701 cgggcgtgcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggtgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt gggtactgtc gacggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc aaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacacca tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 tgatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtccca ggggcgaacg acgggtccag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac tttaggggcg gacaacacag taaaaggcaa ttttttactg tgttcggtgc
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcagcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggtg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggt tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gtttcagtca gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcatcttt ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccagcacag
    13621 gcttacccca atggtcgtcg gcatcgacat cgtaaacctg ataaccgcgc tgctccaaca
    13681 ttttcacagc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gcagcggcac tcagagaaac aacggcagcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctcc gttttcctta tacttcttta gtttcggttg
    13981 ccttctggcg caaacgcaaa cttaattcac gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgg gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggcacc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaaga ccgccgtgag
    14161 gaggtgcgcc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcgcggtga atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg
    14341 ccgggtcgga aaccatcagg tcttcatgac cttcttttgg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaaatca acgacccaca
    14461 aaggtttcca ttcgtctgtg aaatagccgt tgtctttgcc gtgctccaag ccgactttga
    14521 tacgcagtgc gccgatggct tcgttcacga ctttggcttt gtctgcgccg aagaagatga
    14581 tgtcgccgtt ttgcgcgccg gtacgcgcga taatttcttt cagggcgttt tcggacaggt
    14641 atttcacgat tggagattgc aggccgctgt cttcgccgtt ggaaaggttg ctgacatcgt
    14701 ttactttgat gtatgccaga cctttcgcgc cgtagatgcc gacaaatttg gtgtattcgt
    14761 cgatttcttt gcggctgaat tctgcgccgt tcggcacgcg cagagcgacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtaaa tttcaagttg atgcgcatat ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaaatctac atttaaagca tctttgaaga
    15001 cttgtttggc catgccttca gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgggtaaat tcgggctggc ggtcggcacg caggtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg cggtagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcaggcgtg gagcgggtca gcatcggggt ttcaatgtcg atgaaacctt
    15301 gcgcgtccaa gtagcggcga acgcccatag caacttggta acgcaggcgc aggttgcgtt
    15361 gcatcaccgg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtgg cggcggcgtt caagacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tatcgttggt cgtgccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcggga ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgtcgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcactg atcaggccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca atgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gccctgacac gaaagcaatc gtccaaatac cgggctgggg aaacggcacg agtaccggcg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggattt cacaaccgga atccgcccca acaggctgtc ccacgcggcg aggatctgcc
    16261 gacccaatac gttggcggca aacaatccgg ttacaaacaa tacggcaatg gcaacgataa
    16321 cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttgc
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccgg
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaaat cgcacccttt ccgccatttc
    17881 gggattttgc ccccgcaata cagaaaaccc cgaaaccgtc gggcttcagg gttttccgct
    17941 tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccaaggctt ttacttttgc
    18001 agacagacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct tgtcggcgat
    18061 gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt tatcgcctgc
    18121 ttcgactgct ttcaatactt ttttcactgc ggtgcggaat gcggtacgca ggctagcatt
    18181 gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc tgtttgccat
    18241 attggaatct cctggaagaa aaataaattc tgtaaacgcg caattttaaa gggggtttac
    18301 attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc gcaaaaaaac
    18361 ggcaaaatcg ggcggacgtt tttctcccct tttggaaaca ggataatttt ttaggaaaat
    18421 cagtccaata tcgctttgag tttgcacaaa tttactttac aatgcccaaa ccttgcgccg
    18481 gggtaacgat ggggcgcggg gatttctttt tttccaacct ctttcatcag gaatctgccc
    18541 gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc tgcggcggca
    18601 gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat gccgaagccg
    18661 ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgatgag agtacggtcg
    18721 aagactttaa aaaagccaac aatctgaagc tgacttacga cttgtacgaa aacaatgaaa
    18781 cgctggaagc caaaatgctg accggcaaat ccggttacga tttggtcgtg ccgggcatcg
    18841 ccttcctgcc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa gacctgattc
    18901 ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc gacccgggca
    18961 accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg gcgaagggca
    19021 aagagctttt gggcggcaag ctgcccgaaa acggctggga tttgctgttc aaacccgaat
    19081 acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccgagt gaaatgttcc
    19141 cgattttgct gaactacttg ggcaaagacc ccaaaggctc gaatcctgaa gacttgaagg
    19201 cggcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc ccgtccatca
    19261 tcgacgagct ggcacgcggc gacatctgcc tggcggcagg caacggcggc gatttgaact
    19321 tggcgaaagc acgttccgag gaagtgaaaa acaacgtcgg catcgaagtg ctgacaccga
    19381 aaggtatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa aacgtcgcca
    19441 atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa aacggcatcg
    19501 ccgtaacctt tgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc gagctggtga
    19561 acacccgttc catcttcccg aacgagcagg atatgaaaga cggtttcgtg atgccgcaaa
    19621 tgagcacgga tgcgaaaaaa ctgtctgtca gcctgtggca gaaaatcaaa gtcggcacca
    19681 actgatttga agcattaaaa atgccgtccg aacgatgttc ggacggcatt ttatattgga
    19741 ttgaaataga aatatttata tagtggatta acaaaaatca ggacaaggcg ac
//
LOCUS       DAWISC010000019        19237 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWISC010000019 DAWISC010000000
VERSION     DAWISC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.019"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WIW87_000473"
     CDS             complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WIW87_000473"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ5173679.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1948..2217)
                     /locus_tag="WIW87_000474"
     CDS             complement(1948..2217)
                     /locus_tag="WIW87_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ5173680.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2286..2723)
                     /locus_tag="WIW87_000475"
     CDS             complement(2286..2723)
                     /locus_tag="WIW87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ5173681.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DINNNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2805..3368)
                     /locus_tag="WIW87_000476"
     CDS             complement(2805..3368)
                     /locus_tag="WIW87_000476"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ5173682.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            complement(3437..4261)
                     /locus_tag="WIW87_000477"
     CDS             complement(3437..4261)
                     /locus_tag="WIW87_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ5173683.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLMSRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4353..4985
                     /locus_tag="WIW87_000478"
     CDS             4353..4985
                     /locus_tag="WIW87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ5173684.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5192..6976)
                     /gene="rmuC"
                     /locus_tag="WIW87_000479"
     CDS             complement(5192..6976)
                     /gene="rmuC"
                     /locus_tag="WIW87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ5173685.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7315..8100)
                     /locus_tag="WIW87_000480"
     CDS             complement(7315..8100)
                     /locus_tag="WIW87_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ5173686.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEYWKDVH"
     gene            complement(8115..9464)
                     /locus_tag="WIW87_000481"
     CDS             complement(8115..9464)
                     /locus_tag="WIW87_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ5173687.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFAGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDTNKPVPERVTMSTTKQKIMFFVYVGITVVGAYLFATNI"
     gene            complement(9483..10064)
                     /gene="petA"
                     /locus_tag="WIW87_000482"
     CDS             complement(9483..10064)
                     /gene="petA"
                     /locus_tag="WIW87_000482"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ5173688.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10187..10936)
                     /locus_tag="WIW87_000483"
     CDS             complement(10187..10936)
                     /locus_tag="WIW87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ5173689.1"
                     /translation="MVLCRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GNPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11067..11996
                     /locus_tag="WIW87_000484"
     CDS             11067..11996
                     /locus_tag="WIW87_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5173690.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12251..12643)
                     /gene="rpsI"
                     /locus_tag="WIW87_000485"
     CDS             complement(12251..12643)
                     /gene="rpsI"
                     /locus_tag="WIW87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ5173691.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12653..13084)
                     /gene="rplM"
                     /locus_tag="WIW87_000486"
     CDS             complement(12653..13084)
                     /gene="rplM"
                     /locus_tag="WIW87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ5173692.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13262..13441)
                     /gene="ssrS"
                     /locus_tag="WIW87_000487"
     ncRNA           complement(13262..13441)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIW87_000487"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13447..13752)
                     /locus_tag="WIW87_000488"
     CDS             complement(13447..13752)
                     /locus_tag="WIW87_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ5173693.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13763..14092)
                     /locus_tag="WIW87_000489"
     CDS             complement(13763..14092)
                     /locus_tag="WIW87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173694.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14147..15136)
                     /locus_tag="WIW87_000490"
     CDS             complement(14147..15136)
                     /locus_tag="WIW87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ5173695.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15259..17961)
                     /gene="ppc"
                     /locus_tag="WIW87_000491"
     CDS             complement(15259..17961)
                     /gene="ppc"
                     /locus_tag="WIW87_000491"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ5173696.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPGVRVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNGDVTALSDKSPDEEIARAEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKETGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18131..18901
                     /locus_tag="WIW87_000492"
     CDS             18131..18901
                     /locus_tag="WIW87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ5173697.1"
                     /translation="MTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCG
                     GLGAAALPYLAASGVGTLTIADSDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRIN
                     HTVNVRAVNEKLDGCRLTGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVR
                     FEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILL
                     DAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR"
BASE COUNT         4125 a         5234 c         5088 g         4790 t
ORIGIN      
        1 acctagattc ccgcctgcgc gggaatgacg gattttaggt ttctgatttt ggttttctgt
       61 ttttgaggga atggccgatt ttgggtttct gtttcggttt tctattttgc aagaatggca
      121 aaatttcaga ttgcgggcat tgttaagtat ttctattttt tacctgccgt atttatttcc
      181 gccccttgaa gtcggcttct tcctcgacag acacgctgtt catctgtttg atcagctttt
      241 ccgacttctc ttcgtcttcg cagcggatga ctttcacaat atcgctttcg agctgtccga
      301 cattgctgtg cagaatgatg tttttgacgg gcaggatgtt gttggggttc atggaaaaac
      361 ggcgcagccc catacccaat aaaacgcggg taaacgcggt atcgcccgcc atctcgccgc
      421 atacggatac gtctttgtcc atgcggttgg cggtacggat gacgtgttgc agcattttca
      481 gcacggcggg atggccgggc tggtagaggt ggctgacgct gtcgtcgccg cgatcgacgg
      541 acaagatgta ttgaatcagg tcgttggtac cgacggagat gaaatcgacc agtttcaaaa
      601 tactgccgac ggtcagcgcg gcagacggaa tttcaatcat acagccgatg ccgactttac
      661 cgaaggcatc gccgcgttcg gcaagctggc gttgcgcggt gtcgaggtgg atgaggcact
      721 ggcgcacttc ggatacggag gtaatcatcg gccacatcat ccgcacgggg ccgtgtaccg
      781 ccgcacggag gatggcgcgc atctgggtgc ggaacatgac cggttcggca aggcacaggc
      841 ggatgccggt catgcccagc gcggggttga ggctgccgtt gggcgtgctg tttttcccga
      901 accagcgcgg gtttttgtcc acacctaaat cgactgtccg tatcgttacg cttttgcctt
      961 tcattttttt gacaatcgcg ctgtacactt cgtactgctc gtcttcagac ggcatcgtat
     1021 cgcggttcag gtaaagaaac tcgctgcgga acagcccgat gccgtctgcg ccgaggttgt
     1081 gcagcggttt cacgtcttcg gcggattcta tattgcccac aagctcgatg cagaccccgt
     1141 cggcggtggc ggcggcggtt tttttgagct tgttcaaatc gcgtttgtgg ctgcggtatt
     1201 cgcgggcacg gcggcggtat tcgttcaaca ccgactcatc cggcgcgata atcaacacgc
     1261 cgttgatacc gtccacaatg accgtttcgc cttcggtaat cagtttgcgc gcgttgtgca
     1321 gcccgacgac ggacgggatg tccaagctcc tgcccaaaat cgccgtatgc ccggtggggc
     1381 cgccggcatc ggtaacgaag gcggcaatgc gctgctcttt aaacaaaacc gtgtcggcgg
     1441 gcgaaaggtc gtttgcaatc agaacggttt cgtcaaacag gttgtcggca acttccaact
     1501 cgttgccctg cccgatcagg ttgttgtgga tgcggcggac gacttgcagc atatcctgct
     1561 tgcgttcgcg caaataggca tcgtccatat tgtcgaattg ggcggcgagt ttgtcgctct
     1621 gctgcttcaa tgcccactcg gcgttgattt tttgttccct taaaatatcg acgggttcgc
     1681 gcgacaaggt aacatcggtc aagagcatca ggtgtagcga gatgaacgcg cccaactcgg
     1741 tcggggcgtt ttcgggaatc gcgctgcgga gctgttccaa ctctttgcgc gtggctttga
     1801 cggcggcatc gaaacgttcg gcttcggcat cggtgtccgc ctccgcaaca tcatactgcg
     1861 gcacttcctc cgtaccgcgc gcaatcaggt gggcgcaacc gacggcaatg cctttgcccg
     1921 ccgccacgcc gtgcagcacg atactcatta ttcgccctcg ccgaagtagc cgttgattaa
     1981 gtcggtcagg gcgcgcatcg cttccgcctc gtccgcgccg tccgtctcca gttcgatgac
     2041 cgtacccttg gcggcggcga gcatcatcag ccccataatg cttttgccgt tgacgcggct
     2101 gtcgtttttc gtaacccaga cttcgctttt gaattgggac gcggtttggg tgaacttgtt
     2161 ggacgcgcgg gcgtggagtc cgagtttgtt gatgatttcg atggattgtt tgagcatttc
     2221 gattcccgtg ttatgtatat cggcagcaga cgccgtttaa aatgttttcc tgccctgccg
     2281 cttcttcaga cggcatcgcc gctgcgccgg cacaccaaat cttcgggcgc ggacgtgatg
     2341 gcgaaaatgc cttttaccgc cgcctccctg acgcattcgg taaaggcggc aaggtcttcc
     2401 gccgccggcg aatattggac ggccttaacc atcatcggcg cgttcagccc ggtcaaaatc
     2461 gccgatttgt tttcgcgcac gaggcggcgg gcggcattgc agggggtcgc accgaaaata
     2521 tcggtcataa tcagcacgcc gtcgttgtcg ggaaattcct gaagcgcggc aatggcgttg
     2581 ttgttgatgt cgtcttggtc ttccgtcggc tgcacgccga gtatgcggac gttttcaggc
     2641 agtccgcccg gaaaaaaatg atgcgccagc ttgcggtagg cttcgcctat ggtttcgtgt
     2701 gtgatgatta aaagccctat catattatgc gtcctgttcc tcattatcct gccggcgtat
     2761 gggcgcgatg ccgtctgaac agccttcaga cggcatcgcg ccccttattt tccgcccaat
     2821 gcgtaaatct cgcccagatt gcgccagcag cccgccgcat ccatgccgta accgaaaaca
     2881 taacggttcg gcacatccag tccgacataa tcggctcgga taggcttggg tttgtcaatc
     2941 agcttgttgg cgaacaccgc cgcacggcag cttgccgcac ccatttccaa aagtttggct
     3001 tgaatggcgg acatcgtatg cccttcgtcc aaaatatcgt ccagcacgac gacgtgcctg
     3061 ccccggattt gttccgcatc gggcatacgc ttccagttga acgcgccgcc ctccagcttg
     3121 tcgccgtaac gggaaacgtg aacataatca aaatctaagg gaaaacgcaa cagcggcagc
     3181 aactgccccg taaacaccac cgcgccgccc atcacgggca gcagcagcgg atatttgccg
     3241 cccaaatcac gcgtaatctc gtccgccact ttttgcagtg cggcacggca ttggccttgg
     3301 tcgaacaaaa gatcggcgtt ttcaagcatc gcctgtgttt caaggcgttt ggtttctaaa
     3361 tcggtcatat gtcggaatcg gtcggtaaaa ggaaaattat aaaccaaagt atcggatgcc
     3421 gtctgaactg tcctgctcag cggtcggtac gcacgcgcac aaatgtggca aatttcggcg
     3481 tgcctttccg cgtaaagcca cggtaacggt aggtaatcag tgtgccgatt ttgggcgggt
     3541 tgtcgcggtc tttatctttg aaaccgctgc cgatgcggaa ttcgccgtgc cggtttttgc
     3601 agccgaccgc gcccagccgt ccggcgtttc gccctttgcc ctcatagtgc cgcgttaccg
     3661 tgcattcgtc gtcgtattgg cttttcagct tcaataattg gctgctcctg ccgccgctgt
     3721 aacgggattc gggctgacgc agcatcacgc cttcgccgcc ctgcgcttcg atttgtttta
     3781 aaaagtccat cgcgtgctgc cggtcgcgca ctttgatttg cgggatgatg gtaatcggcg
     3841 cgttcggatg cgttttcagc cactgcgttg cgactgccaa acgttggtag aggttgccct
     3901 gcgccttggg tacatcgaaa acgtgcaggc ggatgccgcg ccagtctgaa gaaacagaac
     3961 gcacggtagc ggaaatctgc tcgaactgac cacgtccgct atacaattcg ccgtccaaag
     4021 gataaggcgg aaactgagcg gtaaaacctt tgggcggagc aaacgcgtag ccctgacggc
     4081 tcatcaggtg ctttccgtcc caataggcgc gcacgccgtc gagtttctcg ctcatcgccc
     4141 agccggcaat atcctgccct ttgtattcct gcgccagcat caaatccgcc gcgcctgctg
     4201 atgcagggat gaaaaccgcc gtaaaaatcg gtatgatgcc gccgattgtc ttcttaatca
     4261 tctgattccc ccaatatcaa aacgggcggc aaaccgccat aaaacaaacg gcaaacccga
     4321 tgccgtctga aaaaccgttt aggaacacgc cgatgaccct acgttacgaa atcttccccg
     4381 ttaccccctt ccgccaaaac tgcaccctga tttgggacga cgaaagcggc gaagccgtcc
     4441 tgaccgatgt cggcggcgac gtgccgttcc tgctgcaagc gttggcaaac cgcaaactta
     4501 cgctcacggc aatctggctg acgcacggcc atctcgatca cgcgggcggc gtggtcgaaa
     4561 tgttgaaaac gcataaagtc cctgtcctcg ggccgcatcc ggacgatgaa ttcctgctcc
     4621 aatcgctgcc gcaaaccacc gcgcaatacg gatttcccgt ctcgcccgcc tttgcgccga
     4681 accgttggct cgaagaaggc gaaacgctca cggtcggacg ctatgccttt caagtgctgc
     4741 atattccggg ccatacgccg ggacatatcg tcttttattg tgccgaggcg gaattgctga
     4801 ttgcgggcga cgtgctgttt tacgaaacca taggcagaac cgattttccg cgcggcaacc
     4861 acgccgactt aatcaataat atccgcaaca aattattcac ccttcccgaa accgtgcaag
     4921 ttgtcgccgg acacgggcgt atgacttcca tcggacacga aaagcggcac aatccgtttt
     4981 tctaaccgcc ttccctacgg tcttcagacg gcatcatctg cactgatgcc gtctgaaaca
     5041 caaaaggctc agacaaccgc cgccttgccg gtgtacctcg ccggacaagg ctttggtaag
     5101 tacttcaaac aaacccaaaa tcagaaccag tacagttacg ccgcgctttc ggcattcccg
     5161 ccccggctga aacaatattt ttccgcacaa gtcagactgc ttcatcttct gccgcgtatt
     5221 ccaaagattc cgacaacgcc gttgtttcat tggaacgctc gaccaaatcc cgttgaagtt
     5281 gtttgcctgc cttcacgccc aacagacgca gtttctcggc gcgtccgacc agattcccgc
     5341 gcccttcggc aagttgcttg aatgccgtct gaaaactgct ttgcgcctga tcgatgcctt
     5401 tgccgacgct ttcgagcgtc tgtacgaagc cgacaaactt gtcgtacagc ttgccgcctt
     5461 cgtccgcaat cgccagtgcg ttctgatttt gctgttcgtt gcgccaaata ttcgccaccg
     5521 tcctcaaagt cgccagcagc gtactggggc cgaccagcat aatccgtttg tcgaaacact
     5581 cttggaacaa gcccgcgtca ttctgcaacg ccaacaggta ggccggttcg acagggataa
     5641 acataaagac gaaatccaat gtgttcacac cttccaaatc ggtgtaatcc ttcagcgaca
     5701 agcctttcat gtgtgcacgg atgctggcaa cgtgtgccgc cagttcgcgt gccgccgtat
     5761 ccgcatccgc cgcctgcgtg tagcgcacat aagctgtcag cgagaccttg gaatcaatca
     5821 caatctgctt gttgtcgggc aggttgacca aaacgtcggg ctggaggcgg cgcgtgccgc
     5881 cgtcttcctc ttttcggacg gatgccgcct gaaccacata ttcccgccct ttctgaaggc
     5941 cggaattttc caaaaccgtt tccagaatca tctcgcccca attgccctga accttattct
     6001 gcgtaccggt cagcgcgttg gtcagggcct ttgcctcgct gtgcagctgc gcgttcaacc
     6061 cctgaagccg tttcaattcg ttttccaacg tcagccgctc gcgcgattct ttatcatagg
     6121 tttgcttgac caactcgccg aaaccgtgga tgcgttcgtt tagcgggttc aaaacctgat
     6181 ggagctgctc gcggttctgc tcggtaaaac ggcggctttt ttcttccaaa atcgtgttgg
     6241 caagattttg aaactgatcg ctcaaacttt tgcgcgcctc gcccagcaag gacagcttct
     6301 cttcagaagc aaggcgttcc tgttcgattt gcgttgccaa acgttcgttt tcaaccgcca
     6361 aaccctgtgc cttttcctgc aactcggtat gcgactgcct caaccgctcc gcttccgcct
     6421 ctttttcctg caaatgggca atctgttttt cggctgcggc aaaacggttg ccgacatcgg
     6481 aaaggtcgtt ttgcacgtcg cggacagttt ggcggctttc ttccaaatcg gtttcgattc
     6541 tttggcggat ttggcgttcc aaggcatatt ggtcggcaaa cgccttccgt tcctgtccga
     6601 gctgcgttgc caaacgttcg ttttcaaccg ccaaaccctg tgccttttcc tgcaactcga
     6661 tatacgactg cttcagccgc gccgactccg cctctttttc ctgcaaatgg gcaatctgct
     6721 tttcggctgc ggaaaaacgg ttgcccaaag cataattttc gtcctgcaaa tgccggtatt
     6781 tcccgtccaa caccgccaat tccgacacgg ttttgccgtg tgcctgttcg acaaaatcac
     6841 atcttgccgc cttttccgcc aggtgcgcgt tcaaaccggc aaactcgccc tgaaaccggc
     6901 ccttcatcag caaccatgta aacaacacgc ccgacaccaa cgccgccaaa ggcagcaaaa
     6961 cagtcatcag ttccatcaat tatcctaata ttcaaacatt ttcacaccgg acaaaaccgc
     7021 cgcttattcc gattctacct gtttgttcga catagctcca aaaaatatag cggattggct
     7081 ttaaacctgt tcgacatcgc cttaccatgc tgcttgcggt ttcagacctt ttcctaattc
     7141 aatatcaatc tgccacaaac cctgattaag ttcccgatgt ctgacatttt tagaatgatg
     7201 ccgtctgaaa tgttgcagct atgttcagac ggcatacgga ttcaggcttt tcaaacggca
     7261 ggcaaaatga aaaaagggca aaccctaaag gatttgccct tttgttccaa acgcttagtg
     7321 tacgtctttc caatattctt tatttaggaa gtaagccaaa ggcagcataa ccgcaaatag
     7381 gaaaatcatc acgacatagc ctatacgttt gcgttgcagt tgtgcaggtt cgcccatgta
     7441 cacaaggtaa ttgaccaaat cgcgtacata tgcgtcgtac tctttttgga tcactttgcc
     7501 gttaggcagg cggcggctgt gcaaaccggt agattcccaa tacagcttag gcttcatctc
     7561 gccgtgttcg tcttttacca taaccggctg acctttggca tccaactcaa cggcttgaac
     7621 accttgttgc tcccacaacg ggtggggcat accgacttta tcgaatacag tattgttcca
     7681 gccgctcgga cgggtcggat ctttatagaa gccgcgcata taggcgtaaa ggtagtctgc
     7741 acctttggaa cgcgcaatca acgtcaaatc gggcggagca gcaccaaacc attttgccgc
     7801 atctttcggg ttcatcgccg aatgcatgac atcgccgaca ttatcggtgg taaacatcag
     7861 gtttttcttg atttcttcgt cagtcaaacc gatgtctttc agacggttga agcgcatacc
     7921 gcttgcagag tggcaagaca aacagtagtt tgtaaagatt tgcgcaccgt gctgcaggct
     7981 gacttggtca cgcaggtcga tatcgacttt ttcgtagtgt ccgccgccgc tggcgacggc
     8041 tgcactcata ggcactgcca gcaataaggc agcaaaccag tttttcagag tttgtttcat
     8101 tttcgctgcc ctcatcagat attggttgca aacaagtaag caccaacaac ggtaataccg
     8161 acgtaaacaa agaacataat tttttgttta gtagtgctca tggttacgcg ttcaggaact
     8221 ggtttgttgg tatccagttt ggtatagaac ggcataccca ggaagaatgc aaagtagacg
     8281 aaagacagga tacgtgcaac caaagtacgc gtatcagttg ctaccattgc acccaaaata
     8341 cccaaaccga tgaaggcaat gatgaacaga accaatgcgg ttttgaagat tgggccgcga
     8401 tagcggacag atttaacctc gcctttatcc aaccaaggca gcaaggcgat cagtacaact
     8461 gctgcaccca taccgattac accccatacc tgagtaccgg caaaggaagg aatcgcacgc
     8521 agaattgcgt agaacggagt gaagtaccat accggcgcaa tgtgcggagg tgttttcagc
     8581 gcattcgctg catcgaagtt tggcgcttcc aagaagtagc cgccgccttc aggtgcaaag
     8641 aacatcacgg cacagaagac aatcaagaat atcgttactg ccaatatatc atgcacaaca
     8701 taatacggaa aaaaaggtat gccatctaga gggacaccgt tttcatcttt cagctttttg
     8761 atttctacac cgtcagggtt gttggaaccc acttcatgca aggcaatgat atgagccaca
     8821 accaagccga gcaataccaa aggtacagcg ataacgtgca gggcgaagaa tcggttcaaa
     8881 gtaacatcgg aaacgttgaa gtcaccgcgg atccaagtgg acaaatcagg accgataaca
     8941 gggatggcgg agaacaggtt aataattacc tgcgcacccc agaaggacat ttgaccccaa
     9001 ggcagcaggt agcccataaa ggcttctgcc atcaatgcca agaaaatcag ggaaccgaaa
     9061 atccacacca attcgcgcgg ttttttgtac gaaccgtaaa tcagaccacg gaacatgtgc
     9121 agataaacga cgatgaagaa gaaagatgcg ccggtagagt gcatatagcg gataatccag
     9181 ccgccggaca cgtcgcgcat gatgtactct actgcggtaa aggcagcagg cagatggtag
     9241 gcgttaaggt tgccgtccgg tttgtagttc atggtcagga aaataccgct gacgatttga
     9301 atcaccagca ccagcataga caatgagccg aagaaatacc agaagttgaa gtttttaggc
     9361 gcatagtatt cagacagatg ctctttccac attttactta atggaaaacg ggcatctacc
     9421 cagcctaaca atgcttttgc tttgctattg gtttggtttg ccataattat cgttccttat
     9481 tcttagtctt cgcccaccaa gatagttgtg tcgctcaagt atttatatgg cgggacaacc
     9541 aggttggtcg gggcaggaac acctttatat acgcggccgg ccaagtcgaa tttcgaaccg
     9601 tggcaggggc agaagaagcc gcctttccag tctgcaccca aatcggcggg ggcaatgtcg
     9661 ggacggaagg tgggcgagca gcccaaatgg gtgcagatac cgatggcgac aaggatgttc
     9721 ggcttaatcg aacgggtctc gtttttagca tactccggct gctgttccgc atcggaattg
     9781 ggatcggtaa gttcgccgtt caggcctttc aggtctttaa gctgctgatc tgtacggttg
     9841 agcacccaaa tcggtttgcc ttgccactcg gcggtcagca gctgacccgc ttcgatttta
     9901 ctgacatcca cctcgacggc agcaccggcg gccttggctt tttccgaagg gaaaaaactg
     9961 gccacaaacg gcgttgccac acccaatgct gccactccgc ccgcgccgca ggtcgcgagt
    10021 gtcaggaaac ggcggcggcc gttgttgatt tcttgattat ccattattca gtcgtcctaa
    10081 tattttggga ataccgagcc attaaacgtt gcaattttac ccagtttgca gtgatactca
    10141 aagcattatt taaaataagg taaagtttta tgatatttct caagactcaa gccggattgt
    10201 tttcgtcaaa atggcacact tccaacccga aaacctctgc cgccgattct gccagcgcgc
    10261 gtacgccgta acgttccgtc gcgtgatgcc ctgccgaaat gaaagccgta cccgtttcat
    10321 tggcaaggtg gtattgggct tcagagattt cccccgtcaa atacagatcg acaccttcgt
    10381 ctattgccgt ctgaaaaaac ccctgcgccc cgccgctgca ccatgcaacc cgtcggattt
    10441 cgcgttcggg attgccgata acgacaggct tacgttgcaa aactgtttca atatgcgccg
    10501 ccaatgcgcc gagtgtcttg gcttgtttca ggctgcccga gttgagcagg ttttgttcgc
    10561 cgaaccgttt ttctgtcgca aaacccaatc tgtcggcgag ttgggcattg ttgcccagtg
    10621 tgggatgtgc atccaggggc agatggtagc ctgccatatt gatgtcgtgc cgtaacagtg
    10681 cggcaatccg ttcttttttc caaccagtaa cggtcggcaa ctcgtttttc cagaacatac
    10741 cgtgatgtac caaaagcaaa tctgccttct gctccacagc aaaatcaatc gctgccctgc
    10801 ttgccgttac cgacgtaacg attttcccga tatattccct cccttcaacc tgcaaaccgt
    10861 taggggcgta atctttaaac aacgctgtct gcaatgtttc attacaccaa gtcagaaaat
    10921 ccctgcacaa taccatcttt tttcctaatc gctttaaaca agcgggcatt ctaatcgcaa
    10981 aatgtccgga attcacattt ttccgatttg cacccgcata tgaattattt taatatgcgc
    11041 cggttcaata tgccgtctga agccccatgg attccattat cgaattgcgc cacctcaaaa
    11101 ccctgctggc acttgaagaa accggcagcg tctcccttgc cgccaaacgg gttttcctta
    11161 cccaatccgc cctttcccac cagatccgta tgctcgaaaa ccactacggc acgccgctgt
    11221 tcgaacgcaa atccacgccc ttgcgcttta ccccggtggg cgaaaggctg ctgcgcctcg
    11281 cccacgaact tatacctcaa gttgctgttg cagaatggga tttggcgcga atcacggaag
    11341 gagaggcggg agagctgcgg attgccgtcg aatgccatac ctgtttcgac tggctgatgc
    11401 ccgccatggg cgaattccgc ccgatgtggc cccaagtcga attggatatc gtatcgggat
    11461 tccaagcgga tcccgtcgga ctgctgctgc aacaccgtgc cgaccttgcc attgtttccg
    11521 aagcggaaaa acaaaacggt atttctttcc aaccgctgtt tgcctacgaa atggtcggca
    11581 tttgcgcacc agaccatccg cttgccgcca aaaacgtttg gacggcggaa gactttatcg
    11641 gggaaaccct gattacttat cccgttcccg acgagatgct ggatttgccc aaaaaaatcc
    11701 tgattccgaa aaacatcaac ccgccgcgcc gacacagcga gctgaccatc gccattatcc
    11761 aactggttgc cagcagacgt ggcattgccg cccttcccta ttggacagtc atgccctacc
    11821 ttgaaaaagg ctatgtcgtc caccgccaaa ttactgccga cggactgcaa agcaaactgt
    11881 atgccgccat ccgtaccgaa gatacggaca agagctatct gaacaatttt tgccaaatca
    11941 tacgcgaacg cggttttgca gatttgcccg gactgagcga actggaaccg gtctgacccc
    12001 ttattcaacc atacccggca gtttttctat tttttcatgt atagtggatt aacaaaaacc
    12061 agtacggcgt tgcctcgcct tgccatacta tttgtactgt ctgcggcttc gtcgccttgt
    12121 cctgattttt gttaatccac tatactgttt ttgatttttg cccaatctgt aatctttaga
    12181 ttgccaatgg gaaaccgtct actacaaata aaaaaccctg cgataagcag ggttttttga
    12241 atttccaaca ttaacgtttg gagaattgtt ttgcacggcg tgctttgcgc agacccggtt
    12301 ttttacgttc gacttcgcgg gcatcgcggg taacaaaacc agcttgagac aaggcgggtt
    12361 tcaacgcggc atcgaagtcg atcagggcac gggtaatgcc gtggcggatt gcgccggact
    12421 ggccggtttc gccgccgcca acaacattga ctttgatgtc gaaagattcg gcgttttcag
    12481 tcagaaccaa gggttggcga acaaccattc ggctggtttc ccgtgcgaag aattcgtcaa
    12541 cgggacgacc gtttacgatg atttgacctg tacctttaat caggaataca cgagccactg
    12601 aacttttgcg gcggcctgtg ccgtagtagt atttaccgtt catgtcgcgt ccttatttca
    12661 gttccaaaac tttgggttgt tgcgcagcat gggcgtgttc cgcacccgca tacactttca
    12721 gttttttaat catggcgtaa cccagaggac ctttgggcag catacctttt acagcttgtt
    12781 ccaaagcgcg gcccgggaat tgctcttgca tttcgcggaa ggtgcgttcg tagataccgc
    12841 ctgggaaacc ggaatggcgg aagtattttt tatcttcgaa tttggcaccg gttacacgca
    12901 gtttgtccgc attgataaca atgatgtaat cgccggtatc gacgtggggg gtgtattcag
    12961 gtttgtgttt gccacgcaga cggctggcga cttcggccgc aacgcgaccc aagactttgt
    13021 cttgggcatc gatgacgaac cattcgcgct tcacctcgtg gggtttcgct gaaaaggttt
    13081 tcatagtgga aatccagata gatatagaaa gttgtaaatt ttaaagacag gattcgattt
    13141 tgtcaatcgc attaccgcgt tacggaagga ttttccggat ttcggcagac tgcatactgc
    13201 tttttcgggc ggggcggcgg ccaatgtgaa aaaccgcatc gttgcgatgc ggttttgaat
    13261 gggaatcccc gcgagagccg tttcggccga atccgcttga accttgctga caaggcggct
    13321 gcctcgggta gtttcgggtg cgtccgcaaa aggacgctcg cgcccactac tgctcccggc
    13381 aaccttaagc gaacttattg gttcaaagga atatatgcct tcgcggacac cgcagggaaa
    13441 aaggggttat tcctgcgcca agcgggatag tgctttttgg caggcgttgt ccatatcggc
    13501 tattttacgc gcaaaatcgc cgattgccaa atcgccgccg ttcagggagg ttttcaacag
    13561 gtcgtggacg acgttgagcg cggccataat gacgattttt tcgctgtccg cgacgcgtcc
    13621 gccttcgcgg atggcttcgg ctttgccgtt gagcattccg actgcctgca acagtgtgtc
    13681 tttttcttct gccggcgtgt tgacggtcag ccgggcgtgc atgacttcga tgtggacttg
    13741 ttcgatgttc atcctttaat ccttattgct gcgtttcctg ccattggggg aggcgcgctg
    13801 ccagtgcgct gattttttcc ctgctctgtt cgagcaggct gcggtatcgt gtattttctt
    13861 ctgtcaggct gtcaattttg ttttgcaggt cttctttgag tttgccgact tggacgagca
    13921 gggcttcgct gagttcgtcg acggcggttt cgtgttcgag tttttgccgc tcgtgcgccc
    13981 gtttgagttc ggcgacggtt tctttgaggc ggcggttttc gctgacgagg gtttcgaatt
    14041 tttgtaccaa cgtataaacg ctgctttcga gtttttcgat attttgtttc ataaccttac
    14101 ctgtccgtat gccgtctgaa ggcttcagac ggcatctgtc tgttgtttat tcaaaacgcg
    14161 cgctgcgttc catcagtctt tcgacaacct gttgcggggt catttctttg cggatgagtt
    14221 gcagcagagt ttgggtaatc ggcatgtcga tttggtactt acaggcagta ttgaagactt
    14281 cttctatcgt gctgacccct tcggaaacgt gtccgatttc gaccagcacc tgatgcagtt
    14341 ccttgccttc tgccaaaccc aagccgacgc ggcggttgcg cgaaagtgcg ccggtgcagg
    14401 tgaggatgag gtcgccgatg cctgccagcc ccatcatggt tttgggctgt gcgcccattg
    14461 cggaggcaag gcgggtgatt tcagctaatc cgcgcgtaac cagtgcggca cgggcgttaa
    14521 gcccgtactc taggccgtcg gacaatccgg tggcaatcgc cataacattt tttaccgcgc
    14581 cgccaaccgc cacgccgata acatcggtac tgccgtaaag cctcatgacg gtcgtgttga
    14641 gctgcggtac gagttcttca atccactctt ggttttcgga ggcaaggacg acggcgcagg
    14701 gcagttgttt ggcgagttcc tgtgcaaaac tcgggccgga aagtacgccg attttcttat
    14761 tgtcgggcaa tacttctttc aagacttgaa aggtcagcag cccggtatcc tgctcgaatc
    14821 ctttgcaggc ggcgaggacg gggaggtgtc ccgcgccgta ctgtttgagc agctctgcgc
    14881 tgcttctcaa tccggcaacg gaggttacga taaggacaag tccgctgtct ttgagcgcgt
    14941 ctgccaaatc cgcacacact tccaaggttt cgggaaagga aaagccgggc agtccgcgtt
    15001 tgttttcacg cgcttcctgc atttgacgga cttggtctgc gttgcgcgtc cacagggata
    15061 cgcggttgcc gtgttgggaa aaatgcaggg cgagcgccgt accccacgaa cctgcgccga
    15121 taacggtaat tttcattggt cgtctttcaa catatcactg ccgttcactt taaaacaatc
    15181 ggtgtttctc tgcaagtgcg gtcagggaaa tgccgtctga aaggcgttca gacggcattt
    15241 tgccccgatg cggcactatc agcctgtatt gcgcaaacct tgcgccacgc cgttgatggt
    15301 caggtgcacc atcagaaggg cgtgcggatt gtcgggttct ttacgcaggc gtttgagcat
    15361 ggcgacttgc aaaccgttga gcgcgttcag gtagggaatc ctcaaagcga gcgaacgggc
    15421 gaggctgcgg ttgtcgcgca aaagctcttc ggtttgcagt aggtcgagca gtgctttgcg
    15481 gctgcggcgg tattcttcct taatcatccc gaagatgatt tttgccttat cgggcgattc
    15541 gctcaagccg gcatagtttt ccgcgagggt gatgtcggtt ttcgccatca cttgttccat
    15601 attggagagc atggcttgga agaacgggtt gctttgggcg tgttcgcgca gggcggcgag
    15661 cgtttcgggt ttgtcttcgc acaaggtttc caccgcgctg ccgaaaccgt accaagccgg
    15721 cagcatgagg cggttctgca tccaggaaaa tacccacgga atcgcgcgca agtcctgaat
    15781 ccgcgccaag gttttgcggc tggcgggacg gctgcctagg ttgagggtgg cgatttcctg
    15841 aatcgggctg gtttgcagaa agtagtcgat gaagtcggga tgggtaatca gttcgcggta
    15901 gtatttgaac gatacgtccg acaatgcctg catcagtttg gcatcagggt cttttttatc
    15961 cggcaggatg ctggcttcca aagtcgcggc aaccaaggtt tccaagttgc gttgggcatt
    16021 gccggggtcg gcgtatttgg cggtaatgac ttcgccttgt tcggtgatgc ggatttgtcc
    16081 cgccacgctg cccgccggtt gggcgagaat ggcttggtaa gaagggccgc cgccgcgacc
    16141 tacgctgccg ccgcgtccgt ggaacaggcg catacggaca tcgtattttt tgaagagttc
    16201 gaccaagccc aattccgcct gatagaggca ccatgagctg gtaacgtagc cgccgtcctt
    16261 gttggagtcg gaatagccga gcatgatttc ttggatgttt ccacggcttt cgagcagtgc
    16321 atcgtaccag tcgaggcgga acatggtttc catgaccgga caggcgtttt ccaacgcttc
    16381 aatggtttca aacagcggca cgatattgat gcggctgtgc ggtttgccgt tttccaccgc
    16441 caacaggccg gtttctttca gcagcaatgc caaggcgagc aggtcgctgg gttgttcgca
    16501 gttggaaata atgctttgtg ttacggcatc ttcgccaaat tcgtctttga ttttgcgcgc
    16561 ttcgttgaaa attgccagtt cgtggcgggt atggtcgctg tatgtgataa acgggctgta
    16621 cagaggacgt tgatggctca attcgcgcaa cagggcggtt tgtttttgct cttcgttcag
    16681 gcggttgtag tcttccaagc ctgcgtgttg gaaaagctcg gcaaccacat cggcgtgttt
    16741 gcctgcgtgt tggcgcaagt cgagcggcat catgtgaaag ccgaacacgg atacggaacg
    16801 gatgaggtct gccaaacggc cttcggcaag cagacggctg ccgttgtcga taagggaacg
    16861 ttgcaatttt ttcaaatcat ccagaaactc ttgtgccgaa gcataaggct cgagaaagcc
    16921 gaatttgcag cccataccca aaccgagcgc gcgcgctttg cccatagcgc gcgccataat
    16981 gtaggcgatg gcgcggcggt agggttcttc ggcgcgggcg atttcttcgt cgggcgattt
    17041 gtcggacaac gccgttacat cgccgttgac tttgacgcgg cggatggaga gcggcagttc
    17101 gcggtagagt ttgtcgagtt cgccgcgata gaagcggaac acggcatcgg cgtggcggcg
    17161 gaaggcaaag cgcagggttt cggcagaaac aaacggattg ccgtcgcggt cgccgccgat
    17221 ccagccgccg attttgagga tgtccggaac gcggacgccg ggataggccg tctgaaagtc
    17281 gtgttccatc ttgcggtaga gcttgggcag ggcttcgaaa aagctcatcg ggaagatgga
    17341 cacgccgttg ttgatttcgt cgttgacgct gagtttgtgg cggcgcgttt cgctggtctg
    17401 ccacaagccc agcaggatag tgtcgatttc gcggcgcagc cgtgccagcg cgtcggcatt
    17461 ggtgcagcgt tcgcgttgcg gcaacagtgc gcggatgcgg cggttgaagc ttaagacggt
    17521 ttggcgttgc acttcggtcg ggtgcgcggt caaaacggcg gtaacggacg tattgtccaa
    17581 ctgccgctgc accgatttgc cgtcggcttt ccccgctttg agcctgcgga cggtttccgt
    17641 caggctgcct tccgcgccgc cgcgtccggc ttcttcgtgg atttggcggc ggcgttcgtg
    17701 gtgcacgtct tcggcgatgt tcaaaatctg ggcgaacagg ccgcaggcca aggttaaatc
    17761 gtgggtttgt tgttcgtcca attgcggcaa tactttttca atcaatgccg cgctgtcgtc
    17821 ggaagtggac aagagtttga ctgtttcgac aaccaacggc gaggcttctt cgtgcaggag
    17881 gttgaacagg gattgtttca gaaattccgc gtccgccgcc aaagccgcgt cctttggatt
    17941 gttcagaata tgcagttgca tgatttttct ctctcgtctg ccgtaaatat tgtaaatgta
    18001 ccccaaatgc cgcatccgtg ccaaaccgtt cacactttaa ccgcccgtgt cccgaaatgc
    18061 cgtctgaagt tgaacgccgc ccgacggcag cgttacaatc gcccgcaact gtttttttcc
    18121 gaacatcatc atgaccacga ccgaacacga caacgacgat gcattcctgc tgcggtacag
    18181 ccgccacatc ctcttggacg aaatcggcat cgaagggcag cagaaacttt ccgccgcgca
    18241 tattttggtc gtcggctgcg gcggtttggg tgccgccgca ctgccctacc ttgccgcttc
    18301 gggtgtcggc acgctgacca tagccgattc cgacacggtc gaactgcaca acctgcaacg
    18361 ccaagtcgca tttgacgagg gcgatgtcgg caaactcaaa accgaagcct tggcaggccg
    18421 cctgaaacgc atcaaccata ccgtcaacgt ccgcgccgtc aacgaaaaac tcgacggctg
    18481 ccgcctgacc ggtttggttc aagccgccga catcgtttta gactgttgcg acaactacgc
    18541 cacgcggcaa gccgtcaacc gtgcctgcgt gcaaacgaaa acaccgctgg tttcaggggc
    18601 ggcggtacgc tttgaagggc aacttgccgt gtaccgtccc gacttgcccg actcgccgtg
    18661 ttacgcctgc ctgtttgacg gcggatcggc ttcagacggc atctgttctc tcttcggcgt
    18721 gttctcgccg ctggtcggca tcatcggcag tacccaagcg gcggaggctc tgaaaatcct
    18781 gctggatgcg ggcgaaccgt cgcacggcag gctggcggtt taccgtgcct tggaaggggg
    18841 ctggcaatat ttcgacctgc cgcgcaaccc tgaatgcccg gtttgcggca cagcgcgata
    18901 aaccctgccg ccgtttcaga cggcatccaa acggatgcgg cggaacggtt ttaaaaattt
    18961 aaaaatttac atttctttgc aaaaaaaaaa aaaaaaaaat aaacttacct tataattgca
    19021 attgttttag caatgtctgt ttcgcagact cattgagtaa aacgttttcc ccgtaatgtg
    19081 tttggccgtc tgtccccttt gggttcggac ggcttttttt tggctgtgtt tgaatacccg
    19141 gttggtttta tctgtttgca gcgggggaag ccgcttattt ccgttcgggc ggaaaacggt
    19201 tccatcggat aaaaggcatt ttgtccgact gattaaa
//
LOCUS       DAWISC010000020        18797 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWISC010000020 DAWISC010000000
VERSION     DAWISC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.020"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(49..2886)
                     /locus_tag="WIW87_000493"
     CDS             complement(49..2886)
                     /locus_tag="WIW87_000493"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ5173698.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFNLEAKVLANAEVFNFKGEAQLGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WIW87_000494"
     CDS             complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WIW87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ5173699.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(3973..5229)
                     /locus_tag="WIW87_000495"
     CDS             complement(3973..5229)
                     /locus_tag="WIW87_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ5173700.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQNISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5308..6699)
                     /locus_tag="WIW87_000496"
     CDS             complement(5308..6699)
                     /locus_tag="WIW87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5173701.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WIW87_000497"
     CDS             complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WIW87_000497"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ5173702.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WIW87_000498"
     CDS             complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WIW87_000498"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ5173703.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WIW87_000499"
     CDS             complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WIW87_000499"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ5173704.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPTSCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFADDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(9481..9981)
                     /locus_tag="WIW87_000500"
     CDS             complement(9481..9981)
                     /locus_tag="WIW87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ5173705.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WIW87_000501"
     CDS             complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WIW87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ5173706.1"
                     /translation="MKLKQIASALMMLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WIW87_000502"
     CDS             complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WIW87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ5173707.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(13872..15107)
                     /locus_tag="WIW87_000503"
     CDS             complement(13872..15107)
                     /locus_tag="WIW87_000503"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ5173708.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFHPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLRL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15112..15909)
                     /locus_tag="WIW87_000504"
     CDS             complement(15112..15909)
                     /locus_tag="WIW87_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ5173709.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(15912..16658)
                     /locus_tag="WIW87_000505"
     CDS             complement(15912..16658)
                     /locus_tag="WIW87_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ5173710.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WIW87_000506"
     CDS             complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WIW87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ5173711.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(17451..18578)
                     /locus_tag="WIW87_000507"
     CDS             complement(17451..18578)
                     /locus_tag="WIW87_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ5173712.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(18654..18729)
                     /locus_tag="WIW87_000508"
     tRNA            complement(18654..18729)
                     /locus_tag="WIW87_000508"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18694..18696),aa:Thr,seq:cgt)
BASE COUNT         3792 a         4875 c         5567 g         4563 t
ORIGIN      
        1 aggccgtctg aaaaacattt ttcagacggc cttgtttatt caatcaaatc agtctttcaa
       61 cttcgccaac tgattttgaa cttttgccat tttgtcttcc aattccgcca aatcggcttt
      121 gtctttttcc accagatgcg caggggcttt ttcggtgtag ccgggtttgg agagtttggc
      181 gttgagtttg tccaaggctt tttgcagctt ctcggcttct ttgctcaaac gggcggtttc
      241 ggcggctttg tcgatttcga ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg
      301 cgcgtcttcg ctttcgggta gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat
      361 cggcaggtat ttgaggtagt ccgccaagtc gtccgtgctt tcgacaaaca gcggggcttt
      421 tacgttgggc tggatgccca tttcgccgcg caggttgcgg actgcgccaa tcaaatcctg
      481 caacacggtc atttgctcga atgccgtctg aacaatctcg ccgctgtcgg cttcggggaa
      541 gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac ataggagcga cggtttgcca
      601 cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac
      661 gcgcaataag gtatggcgtg tggcgcgttg gcggctggcg cagccggttt gaagctgcac
      721 tttggcgagt tccaaatacc agtcgcaata gtcgttccat acgaagctgt acagggtttc
      781 cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgttcga tggtctgatt
      841 cagacggcct acaatccaca tatcggggaa ggagtagccg cgcggttcgg cagcggttgc
      901 gccgtaaccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg cgttccagat
      961 tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttga ccgaacgccc
     1021 caagctggcg tagctcgcca tagtgaagcg caaagcgtcc gcgcccatac tcggaatgcc
     1081 ttcggggaag agttttttcg tggcttcttc cactttcggc gcggtttcgg gtttgcgcag
     1141 gccggtggtg cgttttacca gcagtttttc caagccgatg ccgtcgatca aatccacagg
     1201 gtcaatgacg ttgccttcgg atttggacat ttttttgcct tcgtggtcgc gcacgatgcc
     1261 gtggatgtac acgtctttaa acggtacttt gccggtgaag tgggtggtca tcataatcat
     1321 acgcgccacc cagaagaaga tgatttcgta gccggttact aagacattgg acggcaggaa
     1381 ggctttgagt tcgtcggttt cagacggcca gccgagtgtg gagaacggca caagcgcgga
     1441 ggagaaccat gtatccaata cgtcttcttc gcgagtcaag cctgttttgc cggcttgttt
     1501 ttcggcttct tcctgattgc gggcaacata cacattgcct tcgttgtcgt accatgcagg
     1561 gatttgatgg ccccaccaca gttggcgtga gatacaccag tcttggatgt tgttcatcca
     1621 ttggttgtaa gtgttgaccc agttttcagg gataaagcgt accgcgccgc tatcaacggc
     1681 ttttttggct ttatcggcga ggctcaagcc tttgaactcg ctgtccggct cgccgccgtt
     1741 tggggtggcg gacatggcga caaaccattg gctggtcagc ataggttcaa tcaccgaacc
     1801 tgtacggtcg cctttcggcg tcatcagcgt gtgtggtttg atttcgacca agaaaccttg
     1861 ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtctaagc ctgcgtattt
     1921 ttcaggcagg gcaaagccta gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc
     1981 tagcactttg gcttccaagt tgaacacatt aatcaggcgc gtgtcgtggc gtttgccgac
     2041 ttcgtagtcg ttgaagtcgt gtgcaggcgt gattttcacg cagcctgtgc cgaagtcttt
     2101 ttcaacgtat tcgtcggcaa tcacggggat agtacggccg gtcagcggca ggattaattc
     2161 cttgccgatt aagtgggtat aacgttcgtc ttcaggattg acggcaacgg caacgtcgcc
     2221 cagcagcgtt tcaggacggg tggtcgccac gataacggct tcggcgggat tgtccgccag
     2281 cggatagcgg atgtgccaca tagagccttg ttcttccacg ctttccactt ccaaatccga
     2341 taccgccgtg ccaagcacgg gatcccagtt caccaagcgt ttgccgcggt aaatcaagcc
     2401 ttgctcatac aggcgcacga acacttcggt tacggtttcg gcgcgcacgt cgtccatcgt
     2461 gaaatactcg cgcgtccagt cggcagagca gcccacgcgg cgcatttgtt gggtaatcgt
     2521 gccgccggaa acttctttcc attcccacac tttctccaaa aatttttcgc gacccaagtc
     2581 atggcgggac acgttttgcg cagcaagctg acgctcaacc acaatctgcg tggcgatgcc
     2641 cgcgtggtct gtgccgggaa tccaggcggt gttgcagcct ttcatgcggt agtagcgggt
     2701 cagaccgtcc ataatggttt ggttgaaggc atgacccatg tgcagcgtgc cggttacgtt
     2761 gggcggcggc agttggatgg agaaagacgg tttcgtcaaa tccatatcag gttggaaata
     2821 gccctgctct tcccagtttt gataatgttt ggattcgatt tcggctggat tgtatttgtc
     2881 taacatgatg gaactttgtg aaattaaggt tatttttgat gtgcggatta taacgcaaaa
     2941 aggccgtctg aatcatttca gacggccttt ggcatacagg ttttaaaaat ggaacaatac
     3001 caggctgacg gcaatcaccg ccatacccgt tgtcaggccg taaacggttt catggccgtc
     3061 tgaatagcgt ttggcagccg gcagcagctc gtccaacgcc aaaaacacca tcacaccggc
     3121 tatcacgccg aataccgaac caaacacggc aggcgacaaa aacggctgca aaaccaaata
     3181 gcccaaagcc gcccccaacg gctcggccaa gccggatagc agacacgccc acaccgtttt
     3241 cttacggctg cgggtggcaa aataaaccgg cgcggcgatg gaaatgccct ccggaatatt
     3301 atggatggca atcgccaagg ccaaaggcat cccgactgct ggattttcca atgtggcaaa
     3361 aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc
     3421 gactcgcgcg atatggcggc gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt
     3481 ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg ccgcccatcc cggccaaaaa
     3541 tgccatggtc gccgccgcaa acgcgtggtc tttatcataa atttcagcga acgcctcgct
     3601 ggacttactg aaaatctccg tcagggaaac atataccatc gcaccgccgg caaacgccaa
     3661 accaaacgac aacacacgcg gattgggcgt tttggaaaac atcaccaagc cactgcctaa
     3721 tacggtaaac aaaccggcag ccaatgtgat ggaaaaggca acggccaaat tggacatcga
     3781 aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca ggttcagact aacacttttt
     3841 aatgtatatg ataatagtta ttatttattt tattgattgg atacacggat tttgaaacaa
     3901 aaggccgtct gaaaaatgat tttcagacgg cctttaaatt tgaaatgccg ctaaacctta
     3961 gtgctttcca gcttaagcct gataacgcga caggctcaaa tcgtcgctgc ggatttcggt
     4021 gtctttgccg ctcacgatat cggcggttaa ttttgccgaa cccagcgaca tggtccagcc
     4081 taaagtaccg tggccggtat tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg
     4141 cgtgctgtcg ggcgtcatcg gtctgaggcc gctccagaac gatgcttggc tcaaatcgcc
     4201 gccttccggg aacaagtcgt tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag
     4261 tttgatttcg tagcccgaca attccgccat accgccgacg cggattctgt tgtcaaagcg
     4321 cgtgatggcg actttgtagc tttcatctaa aacggtggac accggtgcgc cgtctgaatt
     4381 ggtgaccggc agggtcaagg aatagccttt gacgggataa atgggcagat tgagatccaa
     4441 ctgcgccaaa accgtcctgc tgaagcaacc gagcgcgcag acaacggcat ctgcttcaaa
     4501 ccgccctgtt tcggtttcaa cggttttgat gcgcagcccg ttgtggtcga tgcggctgat
     4561 gttttggttg aaatgaaacc gtacgccctt ttcctgacac aatttgtata ggttttcagt
     4621 gaagaggcgg cagtcgccgg tcgcatctgc aggcaggtgc aggccgccgg caattttggc
     4681 ggtaacgcgt gccagcgcag gctcaaattc tgcacattct tcgggtttca gacggcggta
     4741 cggcacgccg tagcgttcca aaacggcaat gtcttgtttt gccgcttcga cttctttggt
     4801 ttggcggaaa atctgcaacg tccctttttt gcgtccctca aaattcatgc cggtttgcgc
     4861 ttcaaaacgg cggaacattt cacggctgta ttcggaaatc ctgaccatgc gctctttatt
     4921 ggtttgatag tgcgctgccg tgcagttttg cagcatttgc cacagccatt cgatttgata
     4981 caggctgccg tcggggcgaa acagcaaagg cggatggctt ttaaacagcc atttcagcgc
     5041 tttggtcggg ataccgggtg cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc
     5101 gttggcaaaa ctggtttcca tcgccacacc ctcggcgcgg tcgatgaccg ttacttcatg
     5161 tccggcctct gccagatacc acgcggaaga cacgccggca acacccgcac ctaaaacaag
     5221 cactttcatg tttctccctc cggctttttc aaaacagact taatatgccg tgccgtctga
     5281 atattcggat tcagacggcc tcggatatta atgcggcaat tcgccgtttg tgattttttg
     5341 tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc aataaaagtg caatcaggtt
     5401 gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa
     5461 cacggtaccc agcataacgg aagaaacata acccacgcgg tacaaaccgg caaatttctc
     5521 gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac cagcccaaaa tggttgagta
     5581 ggcaaagaaa atcaggccga tggtaacaat ccagccgccg atgccgggca gcattttttg
     5641 gaatgtgacg gttgtcagtg ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc
     5701 gccgagcagt cccattacca acacgatgcc ggtaatcgag caaacgacga tggtatccaa
     5761 aaacgtaccg gtcatagaaa ccaaggcctg acggacggga tggtcggttt tcgcggctgc
     5821 ggcggcaata ggcgcagaac ccatacccgc ctcattggag aacacgccgc gcgccacgcc
     5881 gtagcggatg accgtaccga tagcaccgcc cgccactgcc tgcgcgctga acgcatcgga
     5941 gaaaatcagc ttgacggcag gcatcagtgc atcggaatta atcgcgataa tggaaagacc
     6001 gcccaacaca taaaacaccg ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc
     6061 tttaatacca cctaaaacga caacggcagt cagaacggtc aacgtaatgc cggtataggc
     6121 aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc gagttggact gcaccgagct
     6181 gccgataccg aaggaagcga atgtgccgaa cagcgcaaac gcgacggcca tccatttcca
     6241 gtttttgccc aagccttttt cgatgtaata catcgggccg ccggacattt cgcctttgga
     6301 attgttgacg cggtatttca ccgccaacac gccttcgccg tatttggtgg ccatgccgaa
     6361 aatggcggtc atccacatcc aaaataccgc gcccgggccg ccggttacca ccgcagtcgc
     6421 cacgccggcg atgttacccg tgccgatggt ggcggacagc gcggtcatca acgccgcaaa
     6481 atgggaaata tcgccttcgt ggccttcgcc gcttttatgc ttctttggcg gcataaacgc
     6541 ctgtttcagc gcataaccca acatcgtgaa ctgcaaacct tttaataaaa cagtcagcaa
     6601 aatacccgtg ccgaccagca gcatcagcat caaaggtccc caaacccagc cgctgacggt
     6661 ttcaaaaaag gctttgggat tgtctaaaaa cacttgcatg gctttctcct ttgtctgttt
     6721 tatttttaaa acaccacttt tgtagtgtcc agtaatttca gcacagaata tccaataaga
     6781 caatatgttc ttttgaaaaa tacttttggt tttttcgccg aaaacaggac ggttcaagtt
     6841 gcggaaattg tttgcaattc tttaaaagca gcggcggagg tcacaatgaa atgtccgaat
     6901 ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac tgccatactt ctgaaatcta
     6961 caaaatgatg catcgatcaa acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg
     7021 ctttcggggt ttcagacggc atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat
     7081 tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg tttcggcgcg gcggagaatg
     7141 tctgccttgg cttcttcaaa cggaatgccg cgatggtaga gggttttgta cacgtctttg
     7201 acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc
     7261 gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt gtgtacgcct
     7321 gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga attggaaaac cagcgtgtag
     7381 ccgcccaaaa cgacgtagtc gccgatggta acgtgtccgg caagcgaggc gttgttggcg
     7441 aaaatggtgt ggttgccgat gacgcagtcg tgcgcgaggt ggcagtacgc cataatccag
     7501 ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tacctaaatt aaaggtggtg
     7561 aattcgcgga tggtgttgcc gttgccgata atcagcttgg tcggctcgtc gcggtatttt
     7621 ttgtcctgcg ggatttcgcc gaggctggca aattggaaaa tgcggttgtt ttcgccgatg
     7681 ctggtgtggc cgttgatgac ggcgtgcgga ccgatttcgg tattcgcgcc gatttggacg
     7741 ttggggccga taacggtgta cgcgccgact ttgacgccgg agtcgagttc ggctttgggg
     7801 tcgatgacgg cggtcgggtg gatgagggtc atgtttttcc tttcctgtcg tgttgccgcg
     7861 aagatgcgcg acggcaacag gttgtctgaa aactttcaga cgaccttttt ctgaacactc
     7921 aaaccacgcg tttggcacac atgatgatgg cttcgacggc aacttgcccg tccactttgg
     7981 caacggcgtt gaatttgccg atgccgcgcc ggctggtcag cagctcgact tcaaagacga
     8041 gttggtcgcc ggggatgact tggcgtttga aacgggcttc gtctatgccg gcgaagaaga
     8101 agaattcgtt ttctttgcgc ccgccttcgc tcaaaatcgc caacgtgccg cacgcctgcg
     8161 ccatcgcttc gatgatgagt acgccgggca tcacgggcag gtcggggaaa tggccttgga
     8221 actggggttc gtttatggtg acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg
     8281 cggtgatgcg gtcgagctgg agaaacggat agcggtgggg gatgagtttt tggatgtctt
     8341 tggcttcgat ggggagttgt acgtccatgt ctgtcgtatt ccttgaataa agtcggtttg
     8401 gttatttgct gtcttgaccg gcatctgaaa gctgctgctc cagtgttttg agccgtttgt
     8461 tcatttcgct taagcggtgg atgtaaacag cgttgcgcgc ccattcttta tgggtggaca
     8521 tcgggaagat gccggcgagg tgtttgccgc tttcggtaat gctgtgggtg acggacgtgc
     8581 cgccgccgat ggtggttttg tcggcgattt cgatgtgtcc gaccgtaccg acgccgccgc
     8641 cgatgatgca gtagctgcct atggttacgc tacctgagat gccggttttg gcggcgatga
     8701 cggtgtgcga accgattttg cagttgtgtc cgatttggac ttggttgtcg attttggtgc
     8761 cgttgccgac ggtggtgtcg ctcatcgcgc cgcggtcgat gttggtgttc gagccgattt
     8821 ctacgtcgtc gcccagcgtt accgcgccgg tttgcgggat tttgaaccac gaatcgtcgg
     8881 cgaaggcgag tccgaaaccg tccgcgccga tgaccgcgcc gctgtggatt tcgacgcgtc
     8941 tgcccagtgt gcagccgtaa taaacgacgg cgttgggatg caggacgact tcgtcgccca
     9001 gtttgcaatc gtgttggacg acggcgtttg ccaagatgcg gcagccttcg ccgagcacgg
     9061 tgtttgcgcc gatgtagacg ttcgcgccga tttcgcagct ggtgggaacg gtcgcgcccg
     9121 gttcgacgac ggcggtcgga tggatgccgc cgcgcgcttt gacgacgggt gaaaacaggc
     9181 gggcgacttt ggcgaaatag agataggggt cgtcggcgac aatcaggttg cgcccttcaa
     9241 atccgtctgc cgctttggcg gaaacgatga ccgcgcccgc gctgctgtcg tggacttcgg
     9301 ctttgtattt cggattggca aggaagctga tgtgttccgc ctgcgcgtct gcgagcgggc
     9361 gcacggcggt aacggaaatg tcctcgccgc gccattcgcc gccgagccgc gcggtgattt
     9421 gggacagggt gtaggtggcc ggaatcatgg ttttcctgtt cggtatgccg tctgaaaggg
     9481 tcagcgggcg ttcatttctt taatgacgct gtcggtaacg tcgtattggg tgttgacgta
     9541 aatcacgttc tgcaaaatga catcgtaacc ttcctgtttg gcgattttga cgatgacgcg
     9601 gttggcgttt tgctggaggg aggcaaactc ttcgttgcgg cggaggttgt agtcttcttc
     9661 aaactgcgcc tgttttttgc ggaacgctgc gaccagcccg cgccattttt cttcggcttg
     9721 cgcctttttt gcgtttctga gtttgccttc ggcaagctgc ctttccaaat ccagaccttc
     9781 gcgttgcagt ttttgcaatt cgtcctgacg agcggaaaat tcgctgtcca gcgttttttg
     9841 aatcttgcgc gcctgcttgg attcgaggta gatgcgctcg gtgttgataa agccgatttt
     9901 ttggaaggtg tcggcgtgcg cgcctgcggt gcagcacaaa ccgatcagag ccgcggcaaa
     9961 cgcgcgggtc aaacgggtca tggtaaaact ccttcgaatg ttgccgcgaa atgccgtctg
    10021 aagggcttca gacggcattt gcgggattag aacgtcgtgc cgagttggaa ttggaagcgt
    10081 tggatttcgt cttccggttt tttcttcagc gggtaggcgt agctgaattt catcgggcct
    10141 aaaggcgaga gccaggtaac cgcgccgccg gcggaatagc gcaattcgtt ggtaaaggtg
    10201 gatttatggg tattgccggc gccgtaaatg ttttgaaccc tgccgccggt cgcggaactg
    10261 ctgttgtcgt cgtaggtttt gccgtcccac acgctgcctg cgtcggcaaa caggctcagg
    10321 cggacggtgc gcgcgtcttt cgcgccgggc atcgggaaga gcagctcggc ggagacgttg
    10381 gcttttttgt tgccgccgta gctgattttt tcgccgtatt cgtcatagac tttcggaccg
    10441 agcgtgccgc tttcgtatcc gcgcaccgaa cccaggccgc cgccgtagaa gttttcaaag
    10501 aaggggattt ctttggttct gccgtagccg cccgcaatgc cgacttcgcc gccgagcatc
    10561 agcgtgaagg ttttgctcag ggggaagaac caggtttggt tgtgggtggc ggagtagtat
    10621 tgcagtttgc tgccaggcag ggcgatttcg gcgttcacgc ccgtcaggta gccgcgcgtc
    10681 ggccataacg cgctgtcggt tttgttgcgc ccccagccga cggtaccttt gtacagccag
    10741 cctttgaagc tgccgtctgt gccgtcggtt ttgccgtatt tcttgataaa gtcggcatag
    10801 tgtttgggcg ctttgttgta ggtgttgacg gtcaggtgtt ctgccaccaa accgaaattc
    10861 acgcggtcgt attcggtaac aggcacgctc atgcggatgc ctgcgcctgc cgtggtggtt
    10921 ttatattgtt tgatgctggt cgatgctttg cgcgggtcga aggcttttcc gtaaacatcg
    10981 tagcccaggc tgaccccgtc tgccgtgaag tacgggtcag taaacgacag cgagccgtta
    11041 agcgtggttt tgctcctgga ggcgcgcagt gcggccgact tgcccgtacc gaacaggttg
    11101 tcttgggaaa cgcctgcgga catgaccaac ccggtatctt gaacccaacc cgcgctcaaa
    11161 tccagggaac cggtggaacg ttcggtcaga ctcatgttca aatcgacttt gtcgggcgtg
    11221 ccggcaagcg ggacagcatc aaactggaca ttgtcgaagt agcccaaaag ctcgacgcgc
    11281 tctttggaac gttgcagctt ggaggtgtcg taaggtgcgg attccatttg gcgtaattca
    11341 cggcggacga cttcgtcgcg ggttttgttg ttgccggtga tgtgtatttc gttgacgtag
    11401 attttccggc ccggttcgat gtgcaggacg aaatcgacgg ttttggtttc agcgttcggc
    11461 agcggctgta cgctgatttc gctgtatgcg tagcctgccg agcccatgcg gttctgaatc
    11521 tcacccaaaa cggcggtcat ctgctggcgt tcgtaccatt tgccgggctt catggtcagc
    11581 agtttttcca gttcggcttt ggggacttcg ttggtgtcgc cttcgatgga gactttgccc
    11641 caacggaaac gtccgccttc gtggacggtg attttgatgg tctgcttggt tttgtcttcg
    11701 ttggtttgga tgtcggtatc gaggatacgg aaatcgaagt agccgttatt ttggtagaag
    11761 tcggttactt tttccatatc ttgggcaaat ttctgctcgt tgaattggtt gcttcgtgtc
    11821 agccatgtcc aaatgccgcc ttcggtcagg gacatttgcc gcatcagttt gcggtcggaa
    11881 tagacttggt tgccttcaaa ttcgatgtcg gtgattttgg cggatttgcc ctcgtcaatc
    11941 gtgatgtcga tgtcgacgcg gttgcgggcg agtttggtta ctttgggcgt gatttggata
    12001 ttgagtttgc cgcgcccgag gtattcttct ttcaggccgg cgactgcctg attgagtgtc
    12061 gcctgattaa agtattgcga ctgcgccagc ccgaacgatt cgaggttttt cttaatggcg
    12121 tcgttttgca gcatttttgc gccggtgatg ttgagcgagc cgatggtggg gcgttcgata
    12181 acggtcagca ggagctgccc gtccgcagtt tcgacgcgta cgtcgtcaaa gaaaccggtg
    12241 gcgtacaggc ttttgatgat ggcactgccg tgtgtgtcgt tgtaggtgtc gccgactttg
    12301 acgggcaggt agttgaatac ggtactcggc tcggtacgct gcaagccttc gacgcggatg
    12361 tcttggatgg tgaagtcggc aagtgccaaa ggcgatatgc ccaacatcat cagtgcggaa
    12421 gcaatctgtt tcagtttcat tgtcagttcc ttgtggtgcg gaatgcggtt tcagacggca
    12481 ttccgaaacg taaaatctaa ccgagcagcc gggtaacgtc gttgaagaag gcgaccgcca
    12541 tcatcagcat catgagggcg agcccgaagc gcaaaccgat gttttggacg cgttcgccca
    12601 aaggtttgcc gcgtatccat tcggcagtat aaaacacgag gtgcccgccg tccaaaacag
    12661 ggacgggcag taggttcagc acgccgaggc tgatgctgac cagtgctaaa aattccaaat
    12721 aactttgcaa gccgagttcg gcggactgtc cggcaatgtc ggcaatggtc agcggcccgg
    12781 aaatatggct gacggaggcg ttgccgctga ttagtttgcc gaaaaatttg agggttgtcc
    12841 acgagtggga aacggttttt tcccagccca tgccgaatgc gcggacaaca gacggacggt
    12901 agctgcggcg gatttgcgcg tcccacgccc tgtccggctg cggacggagg ccgacgcgcc
    12961 cgatcagggt gtggtcggac tgttcgacag tatcggggcg gatgtcggcg gtatgggttt
    13021 gtccggcgcg ttcgtagttc agggtgattt ttttgccggg gctttggcgg gtcaggtttg
    13081 cccattcttg ccatgaggcg atgggtttgc cgtcggcggc agtcagcctg tcgcccggtt
    13141 tcaggcctgc tttttcggcg gggctgcctt tttccacgcc gccggcaacg gttgtgattt
    13201 taaagggcat cagtccgatg tagccttggt tttttgcgat tttaccggct tccggcgtgc
    13261 ctgcggcatc gatggtgcgg acggtttgcg cgcccgatgc cgtctgaacg ccgacggcga
    13321 ctttgccggc ttcgaggttg aggacgattt cggtttgcgc gctgccccaa tctgcaacgg
    13381 gtgtgccgtt gacggattgt attttgtcgc cgctttggaa gccggcgcgg gcggcaatgg
    13441 tgtcgggttc gactgtgccg acgtaggggc gcagttcggt tacgccgaag gaaaagctca
    13501 gtccgtacag caaaaccgcc agtgcgaggt tggtcagtgg gccggcggcg acgatggcga
    13561 tgcgcttggc ggggtgttgt ttgtcaaaag cgtagggtaa atcggcttct gatacttcgc
    13621 cttcgcgcgt atcgaccatt ttgacgtaac cgcccaacgg aatcggggcg aggcaccatt
    13681 cggtgtcgcc gcgctttcgg gtgaaaaacg gtttgccgaa gccgacggaa aagcgtacga
    13741 ctttgacgcc gcacaatctg gcaacgatgt agtgtccgaa ctcgtgcagg ctgaccaaaa
    13801 tcaggatggc gaagataaaa gctagaaggg tgtgcaaatg gttttccttt gataacggtg
    13861 ttcagatggc atcagcgcag tgtgccgata aatgctcgcg cttgtgcgcg tgtccgggca
    13921 tcttgcgcca agagcccccc tatatcgcct atgccgtctg aaaagtcttg tgcaagacag
    13981 tgggcgacgg ttttggcaat gtcggtaaac ttaatctgtc cgtccaaaaa ggcggcgacg
    14041 gcggcttcgt tggcggcgtt caatacgcag ggcgcggctc cgcctgcgtt catggcttca
    14101 taggcgagcc tcaggcaggg gaagcggtca aagtcgggct tttggaaggt cagcgcggac
    14161 aatgcgtcga aatccaggtc gccgacaccc gaatcgatgc gctcgggcaa acccaaacaa
    14221 taagcgatgg gcgttcgcat atcgggattg cccagttgcg ccagcacgga gccgtcgcgg
    14281 tagcgcacca tgctgtgtat cacggattgc ggatggatga cgacttcgag tttgtcgggc
    14341 ggacagttga acagccaatg cgcttcaatc agctccaaac ctttgttcat catggtggcg
    14401 gaatcgacgg agattttgcg tcccatacgc caattggggt gtttgaccgc ttgggcgggc
    14461 gtaatgcggt cgaacgtgtt taaatcggcg gtcagaaacg ggccgccgga agcggtcagg
    14521 ataatcgaag cgatgccgtg ttcgttcaga cggccggcgt aatcgcgcgg caaaacttgg
    14581 aaaacggcgt tgtgttcgct gtcgacgggc agcactgccg cgccgtttgc acgggcggtt
    14641 tccataaaca acgcgccgga aaccaccagc gtttctttgt ttgccagata aatggttttg
    14701 cctttttgcg ccgctgcgag cgcggaaggc agccccaccg ccccgacgat ggcgcacatg
    14761 acaccgctga cttcgtcggc agaggcaacg tcaaccaatg cctgcgcgcc gtgtaaaacc
    14821 tgagtcgccg tgccgtcgcg tttcaacagg gcttcaagcc gggcggcgtg ttccgcatcg
    14881 gcaacgacgg catattcggg gtggaacgtt tgacattgag ccgccaattt ctcgacctgc
    14941 ttatgccctg ccagcgcgaa tacgcggaat ttttcggggt ggcgggagac aacgtccagc
    15001 gtgctttcgc ctatgctgcc ggtactgcct aatatggtca ggacttgtgg tgtcataatg
    15061 gggataactt tataccggat gccgtctgaa gcgttttcag acggcataga atcaatttaa
    15121 aaccgacatc atcgctgcat agacgctgat aacggcaatc aggctgtcgg tacggtcgaa
    15181 cacgccgccg tgtccgggca gcagcttgct gctgtctttg atgcctgccg cgcgcttgag
    15241 ccagctttcc aaaaggtcgc cgcatacgct gacaacggtc agcaccaaac cgattaacac
    15301 ggtatcgaac cagcctgtat cgaatgccag ccagccggca cttcgtacgg cggtcatgta
    15361 cactgccacg caaaccgcgc cgccgattgc accttcccag cttttgccgg ggctgattgc
    15421 cggcgcgatt ttgtgtttgc cgaacgcctt gccgctgaaa tacgcgcaaa tatcggcaac
    15481 ccacaccaaa cccatcacgg cgagcagcgg cagggcatca tcgggatgcg ggcgcaggga
    15541 tacgagcgcg aaccaaaacg gcatgaccag aagccagccg acggcataaa cctgccaacc
    15601 gccgttgagc ctccatttga atctcaacca taaaggcata acggcgagcc aaaatgccaa
    15661 aacaacatac caaaccaaat taggcagcat ccagccgccc gcataggcaa ccacgccgaa
    15721 aaccaaggtt gcggcgaggt aatggttggt tttaattttg cacaaaccgc ccatacgggc
    15781 atattcccac aaggcaatca gggcaatcag tccgcaaaat gcagcccaca accattgcgg
    15841 cgcgtaaaac agcatgccca gcatcagcgg cagcagccac atggcggtta ttacccgttg
    15901 tttcagcata ttcagttcct ttgctgttcg ataggcagtt gctcggaggt gcgtccgaac
    15961 cgccgttcgc gtttttggaa cgaagcgacg gcatcgtcca aagccttgcc gtcaaaatcg
    16021 ggccacaaaa tatcggtgaa atacagttct gcatatgcca tctgccagag caggaaattg
    16081 ctgatgcgcg tttcgccgcc ggtgcggatg aacaaatccg gttccggtgc atcgcccagc
    16141 atcaagtgtt tcgccagcgt gtcttccgta atctcggata cgccttcggc aatcagtttg
    16201 tttgccgcct gcaaaatatc ccagcggccg ccgtaatcgg cggcaatgct cagggtcagg
    16261 ccggtattgt ttgccgtcaa cgcttccgcc tcttcgatgc cttgcagaat ctgccggttg
    16321 aagcgttcgc ggctgcccaa tatcttcagg cgcatattgt tttcgtgcag gcggcgtacc
    16381 tgtttttgca aagcctgtaa aaacagcccc atcaggaacg aaacttcgtc ttcggggcgg
    16441 cgccagtttt cggttgaaaa ggcaaacacg gtcagatatt gcacacccag tttggcgcaa
    16501 tgcttcacca tattttccaa tgcgtccaaa ccgcgtttgt gtcccattat gcgcgggagg
    16561 aaacgttttt tcgcccaacg gccgttgccg tccataatca cggcgatatg cttgggaatg
    16621 gcggtgtgtt ccaaaacggc ctgcgtgctg cttttcatgt ctgcctttcg cggttcggca
    16681 ttcaaatgcc gtctgaacgc cgaaccgtgc aggttaaatt gccatcaaat cttcttcttt
    16741 ggcagtcagg agtttgtcgg cttcggtaat gtatttgtcg gtcagttttt gaaccgcttc
    16801 ttcgccgcga cgtgcctcgt cttcggaaat ttctttgtct ttgaggagtt ttttgatgtg
    16861 gtcgttggca tcgcggcgca cgttgcggat agagacgcgg ccttcttccg cttcgccgcg
    16921 tacgacttta atcaggtctt tgcggcgttc ctcggtcagc atgggcatcg gcacgcggat
    16981 caggtcgccg acagctgccg ggttcagtcc caagtttgaa tcgcggatgg ctttctcgac
    17041 tttggccgcc atattgccct caaacggttt cacgccgatg gtgcgcgcgt ccagaagcgt
    17101 tacgttggca acttggctga cggggaccat gctgccccag tattcgactt ccacttggtc
    17161 gagcaggccg gtatgcgcgc ggccggtacg cactttcgcc agattttctt tcagtacttc
    17221 gaccgaacgc tgcatcttgc cttcggctgt tttttgaata tcgttgatca tattgttctt
    17281 tcggtgggat aaggtgggcg ggagaccgtc tgaacgcgtt tcaagccgtt cagacggcat
    17341 aaagaccgtt aaccgcgaat agtaccgtta ttcgggcata acgacaaggt aggcggattg
    17401 gggatgccgt ctgaagcgac aggcgtttca gacggcatcg tgtccgaccg tcagccgtgt
    17461 tcccgtgttt caagcaggct ttggcgcagg tgttggcgtt cgtgggcatc cagccatttg
    17521 cggcgggtgc gttgcagcag gatgacgagg gcggaaattt cctgacgcat attggtgctg
    17581 agccagagga agccctgcca ttggtagtgg aggtgttcgg cgagggcttc cagttcgggg
    17641 ttgatggcgg tgtcgatgcg gatgcggcgg gcgtgtctgc cgttgataag ggcgacggtt
    17701 tgttgcaggt cggtttggag cagtgtgaag tggcggtcaa gcagccggat ttcgctgccg
    17761 ttgagtttgg gagattgcag cttggcggcg gtggtcagga gcagctcggt ggtgttgacg
    17821 attttacggt gggcgtgctg catggcttcc atcatggcgg ggctgatgcg gctttcgccc
    17881 gatgtggcgg cgagatggct gcggcttttg accatgcgtg cgttgatttg gcgcattttc
    17941 gccatgttct cctcgaggcg ttcgcgggtc atgcgcctgc cgttgctgat ttcggcaatc
    18001 attttgctgc agtcggccag gttgtcggca agcatgaaac gccacatcag tgtggatttc
    18061 agcggcagca gtttggcggc ggcgatggcg atggccgcgc cgatgaggac gttcatggcg
    18121 cgcatgagtc cgctgtcgag ccattcgctg ccgttgtcgc cgatgagcat acacatcgtc
    18181 agccctgcca gcatagggac gtagccgttt ttgccgaccg ccgcccagcc ggccagtgcg
    18241 cttgccgtgc cgacggtgag gtagaagagg aggttgccgt ggaaataatg ctggttcagc
    18301 cataaaacgc ccaaacccgc gcccagcccg atgaccgtgc cgagcatacg ttccaccgcc
    18361 ttggagtaaa tcgccccttg aaactggagc atgccgagga cgacgaagac ggtcatccct
    18421 atccactcgc cgtgttggag gtggagcagc cgggcggagg cggtggcgaa caggacggcc
    18481 ccgccgagcc ggacggcgtg gatgaggcgg cggtagcggt agcgttcgta ggagttgagc
    18541 cagcggctga cgaggcggtt gcgttgcgag gtgttcatat cggttgtgcc gtctgaagcg
    18601 gaaatgtgaa aaagcacagg cttcccgagg aagggagggt ctgtgcttgg tattggtgcc
    18661 ggagaaggga atcgaacccc cgaccttcgc gttacgaatg cgctgctcta ccgactgagc
    18721 tacaccggcg ttttttcgtc atgatatata tgaacggttg tttgtgcaac ttttcgggcg
    18781 ggcggcaagg cagtgcg
//
LOCUS       DAWISC010000021        18114 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWISC010000021 DAWISC010000000
VERSION     DAWISC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.021"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            155..991
                     /gene="cysT"
                     /locus_tag="WIW87_000509"
     CDS             155..991
                     /gene="cysT"
                     /locus_tag="WIW87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ5173713.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1243..2103
                     /gene="cysW"
                     /locus_tag="WIW87_000510"
     CDS             1243..2103
                     /gene="cysW"
                     /locus_tag="WIW87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ5173714.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAI"
     gene            2100..3173
                     /locus_tag="WIW87_000511"
     CDS             2100..3173
                     /locus_tag="WIW87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ5173715.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKIHAVGALTHILVKHDKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WIW87_000512"
     CDS             complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WIW87_000512"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ5173716.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4904..6073
                     /locus_tag="WIW87_000513"
     CDS             4904..6073
                     /locus_tag="WIW87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ5173717.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(6198..6770)
                     /locus_tag="WIW87_000514"
     CDS             complement(6198..6770)
                     /locus_tag="WIW87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ5173718.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6837..7820)
                     /locus_tag="WIW87_000515"
     CDS             complement(6837..7820)
                     /locus_tag="WIW87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ5173719.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7987..8062)
                     /locus_tag="WIW87_000516"
     tRNA            complement(7987..8062)
                     /locus_tag="WIW87_000516"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8027..8029),aa:Gln,seq:ttg)
     gene            complement(8071..8146)
                     /locus_tag="WIW87_000517"
     tRNA            complement(8071..8146)
                     /locus_tag="WIW87_000517"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8111..8113),aa:Gln,seq:ttg)
     gene            complement(8159..8234)
                     /locus_tag="WIW87_000518"
     tRNA            complement(8159..8234)
                     /locus_tag="WIW87_000518"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8199..8201),aa:Gln,seq:ttg)
     gene            complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WIW87_000519"
     CDS             complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WIW87_000519"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ5173720.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            complement(9097..9678)
                     /locus_tag="WIW87_000520"
     CDS             complement(9097..9678)
                     /locus_tag="WIW87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ5173721.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETLERCAARTR"
     gene            complement(9818..11656)
                     /locus_tag="WIW87_000521"
     CDS             complement(9818..11656)
                     /locus_tag="WIW87_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173722.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11821..12657)
                     /locus_tag="WIW87_000522"
     CDS             complement(11821..12657)
                     /locus_tag="WIW87_000522"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ5173723.1"
                     /translation="MQIIHTVRELRAWRKNAGTVAFVPTMGNLHEGHLELVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(12781..13572)
                     /gene="panB"
                     /locus_tag="WIW87_000523"
     CDS             complement(12781..13572)
                     /gene="panB"
                     /locus_tag="WIW87_000523"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ5173724.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13714..13789)
                     /locus_tag="WIW87_000524"
     tRNA            complement(13714..13789)
                     /locus_tag="WIW87_000524"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13754..13756),aa:Thr,seq:tgt)
     gene            complement(13824..13899)
                     /locus_tag="WIW87_000525"
     tRNA            complement(13824..13899)
                     /locus_tag="WIW87_000525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13864..13866),aa:Thr,seq:tgt)
     gene            14065..14856
                     /locus_tag="WIW87_000526"
     CDS             14065..14856
                     /locus_tag="WIW87_000526"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ5173725.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14911..15552
                     /locus_tag="WIW87_000527"
     CDS             14911..15552
                     /locus_tag="WIW87_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5173726.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSGGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARTPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15630..16553)
                     /locus_tag="WIW87_000528"
     CDS             complement(15630..16553)
                     /locus_tag="WIW87_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ5173727.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16717..17586
                     /locus_tag="WIW87_000529"
     CDS             16717..17586
                     /locus_tag="WIW87_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ5173728.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17629..18063
                     /locus_tag="WIW87_000530"
     CDS             17629..18063
                     /locus_tag="WIW87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173729.1"
                     /translation="MKKQISSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNRNEHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGTVAGGINGYAGSTGKNTDIRR"
BASE COUNT         4436 a         5186 c         4292 g         4200 t
ORIGIN      
        1 ttaatccact atattttaaa acatcgcgcc cgcttgagaa actgccaacc gctttataac
       61 aaattcgtct ttgcaccaaa ctttccattc tttccgtttt tcggacggca tcgttaaagt
      121 agtccttcct tttccttatt ttcagcattg ttttatgtta gcgctcaaaa cgcccggcgt
      181 actgcccggc ttcaaactca gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt
      241 cttgccgttt gcgatgatgg cggcgaaggc ggcggaaatc ggctggggcg gcttttggaa
      301 cacgattgcc gagccgaacg tgttggcggc ggtatggctg agcttgcgga tgtcgtttta
      361 tgcgatgctg accaatgtcg tgttcggcac gctggtggcg tgggtattgg tgcgttatga
      421 attcccgggc aagggtctgg cgaacgcgct ggtcgatttg ccgtttgcgc tgccgacggc
      481 ggttacgggt atcgcgttgg caaccctgta tgcgcccaac ggttggatag gccgtttttt
      541 cgagcctttg ggcatcaaaa tcgcgtttac acccgtcggc atttggattg cgctggtcgt
      601 cgtcagcctg ccctttatcg tccgcgccgt gcagccggta ttggaagaat tgtcgggcga
      661 atatgaggaa gcggcggcaa ctttgggcgc aagccgttgg actacgtttc gccgtgtcct
      721 cttgcctgaa atcacaccgg cactcttgac cggcgcggga atgatgtttg cgcgggcaac
      781 gggggaatac ggttcggtga tttttatcgc gggcaacatt ccgatggttt ctgaaatcct
      841 gccgctgatt attacgggca agctggaaca gttcgacgtg cagggcgcgt cggcggtggc
      901 gttgtttatg ctgctggttt cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc
      961 gttgggcagg cgttcgggcg cgaagggttg aggtcgtctg aaatacctgt taccgtcatt
     1021 cccgcgcagg cgggaatcca ttggtgaatt tcggctgcct tatttatttt ctgttttcct
     1081 gttgccctgc ggtggattcc cgcctgcgcg ggaatgacgg tagctagacg tttttattcc
     1141 cttaatcaat aaaaggttgt ctgaaaacga atccgcccca caaaaaacgg tttttcagac
     1201 ggcatccaaa cattttaaaa ccaaccagag aacaccaccg ccatgaaacc ctattccgcc
     1261 aatcccaacc tgaccgaacc gcgccggctg cgcgtgttgc tgattgccgc cgcgctgggc
     1321 tttctgctgc tgatgctggt cgtgccgctc gtcgccgtgt tttacgaagc cttaaaaggc
     1381 ggttgggatt tgtacctgaa atccttaaac gatcccgaag cgtggtctgc catcaaattg
     1441 acgctgatta ccgcgctgat tgtcgttccc gtcaatgccg tattgggtgt ggcgatggcg
     1501 tggctgctga cccgttttga ttttcgcggc aagcagttgc tgaccaccct gctcgatttg
     1561 ccgttttccg tatcgcccgt ggtggccggt ttgatgttcg tcttattgtt cggcgcgcat
     1621 acggcattgg gtggctggct cgaagcgcaa ggcatacaga ttatcttcgc catccccggt
     1681 attgttttgg cgacgctgtt cgttaccttc ccctttgtcg cacgcgaaat catcccgctg
     1741 atgcaggcac agggcgacag cgaagaacag gcggcattga tactcggcgc aagcggctgg
     1801 cagatgtttt ggcgcgttac cctgcccaac atcaaatggg cgttactcta cggcatcatc
     1861 ctcaccaacg cccgcgcgat gggcgagttc ggcgcggtca gcgtggtatc gggacacata
     1921 cgcggcgaaa ccaacaccgt cccgcttttg gtcgaaatct tctacaacga atacaacttc
     1981 accggcgcat tcgccctctc cggcgtattg gcacttttgg cactggcgac gctggcggtg
     2041 cagaacatca ttaccaaatt acaagacaaa aaactcgccg ccgccgaaag gaatgcaata
     2101 tgagtatcac catccaaaac ttaaacaaac acttcggcaa ttttcacgcg ctgaaaaaca
     2161 tcaacctcaa cgtccccacc ggcaaactcg tttccctgct cggcccgtcc ggctgcggca
     2221 aaaccacact tttacgcatt atcgccggac tggaaaacgc cgacggcggc aatatcctgt
     2281 ttgacgggca agacgtaacc gccaaacatg tgcgcgagcg caaagtcggc ttcgtgttcc
     2341 aacactacgc ccttttccgc catatgaacg tgtttgacaa cgtcgctttc ggtttgaccg
     2401 tattgcccaa gtccgaacgc ccgtccaaag gacaaatccg cgccaaagtc gaagaattac
     2461 tcaagctcgt gcagctctct catttggcaa aatcctatcc gcaccaactc tccggcgggc
     2521 aacgccagcg catcgccctc gcccgcgcgc ttgcggtcga acccaaactc ttgcttttgg
     2581 acgaaccctt cggcgcgttg gatgccaaag tacgcaaaga attacgcacc tggctgcgcg
     2641 acatccatca caacctgggt gtaaccagca ttctggttac gcacgaccaa gaagaagccc
     2701 tcgaagtttc cgacgaaatc gtcgtgatga accacggcaa aatcgaacaa accggcagcg
     2761 ccgaagctat ttaccgcaaa cccgaaaatg ccttcgttac cgagttcctc ggcgaaaccg
     2821 acgcttttga aggacgcatc gaaaaaggct tctggcatta caacggcttc gcgtggaaat
     2881 tggacgcgca atacaaatgg caggaacaaa ccgccaccgg ctatatccgc ccgcacgaat
     2941 ggcagatcgc cgccgaacac gaaacaccga tgatttgtgc cgaaatcgaa aaaatccacg
     3001 ccgtcggcgc attgacgcat attctggtaa aacacgacaa acaggacgta catatcacgc
     3061 tggcaggcag cgatgccgcg cgttacccaa tcgccgaagg caaagaattg aagctgattc
     3121 cgaaacaggt ttatgtcttc tctcaaaacg aactgattga atattcgatt taaccatgaa
     3181 agcgcaatgc cgtctgaaag gctttcagac ggcattgtgc tttcaagcgt caggcaagaa
     3241 acagcttgta cgcggcattt tgcgtttcct cgtgatagct gtatcccaga ctttccaaga
     3301 aaccgtcaaa tgcggcggca tcgtgcggcg gcacatcgat accgaccaaa atccgcccgt
     3361 aatccgcacc gtggttgcgg taatggaaaa gcgtaatatt ccaccctccc tgcatatggt
     3421 tcaaaaagcg tgccaatgcc cccggacgct ccggaaactc aaaactgacc aaacgctcgt
     3481 tttctacttt gtccgtccgc cctccgacca tatagcggat atggattttg gcaatctcat
     3541 tgttggtcaa atcgacattg ggcaatcccg cctcatccaa ccggctgccg ataaccgcca
     3601 aatcctgcgg gcctgccgct tgaagtccga caaagatatg cgctttttca tcgtctccgt
     3661 agcggtagtt gaactcggta atattcctat ttcccaatat attgacaaac ttaaggaagc
     3721 tgccgcgttc ttcagggatg gtaacggcaa aaataccttc gttgccctcg cccaattcgc
     3781 tccgttccga aacgtggcgc aaacggtgaa aattcatatt cgcaccgctg gtaacggcaa
     3841 tcagggtttg gttttccgcg ccttctcggg cgatataggc tttcagaccc gccaacgcca
     3901 acgcgcccgc cggctcggta atgctgcgcg tgtcatcgaa aatatccttg accgcgccgc
     3961 aaaccgcatc ggtatcgact gtaatgattt catccaaaag ttctttgcag aggcggaagg
     4021 tttcgtttcc gacgactttg accgcagtgc cgtctgaaaa cagcccgaca tctttcaaat
     4081 ggacgatttc acccgcttcg accgactgct tcatacagca ggaatcgttg gtctgaacgc
     4141 cgataacttt gatttcggga cggacctgct tgataaatgc cgccacgccc gccgccaaac
     4201 cgccaccgcc tatcggtacg aatacggcgc ggattggatc gggatgctgg ctgacaattt
     4261 ccatccccac cgtcccctgt cccgcaatca catcaggatc atcaaacggc gcgatatagg
     4321 ttaacccttc tttttccgcc aactccatcg cataatcgta ggcatcgttg tatgaaacgc
     4381 cccgcaaaac cacctcgccg ccatggcttt taaccgcatc cactttgatt ttcggcgtag
     4441 tctccggcat aacgataacg gcacggcagc ccaaacgctg tgcggacaat gccacgcctt
     4501 gagcatgatt gcccgcgctt gccgcaatca cgccgcaagc gagcgcatct ttcggcaact
     4561 tggacatttt gttgtacgcg ccgcgtattt tgaacgaaaa aaccggctgc aaatcttcgc
     4621 gtttcaaaag gatgttgttt ttcaaacgta cagaaaggct gcgtgccggt tccaaaggcg
     4681 tttcgaccgc cacatcatag acagatgccg tcaggatgcg gatgaggtaa tcggaataag
     4741 gaaggggcgt gttcataatt caatatggga taatcggttt attaaaatcg caaaacccaa
     4801 aaccatacgc ccaagacggc gcgaaatcaa gaaaaatccg cccgatcaga caccctaagc
     4861 gtataatcgg cagactgaaa cacgcacaca attagaatat ttcatgacag cacataaaat
     4921 cctgcccgtc ctgctttcca tcatcttagg cgtttctcac gcaacggctg catcgcccgc
     4981 gcccaacaga ccgacggtac acgccgcccc cacgttccaa acacccgaaa ccctcacagc
     5041 ggcacacatc gttatcgacc ttcaaagcaa acagatttta tccgccaaaa acatcaatac
     5101 ccctgttgaa ccggcggcac taacccaact gatgaccgca tatctggttt tcaaaaacat
     5161 gaaatcgggc aatatccaat ctgaagaaaa cttaaaaata cccgaatccg catgggcttc
     5221 agaaggaagc agaatgtttg tacgtcccgg cgatacggtc agcaccgaca aactcttaaa
     5281 aggcatgatt gcactatccg caaacgatgc cgccctaacc cttgccggcc ggctgggcaa
     5341 cggctcgatt gaaaattttg tgcaacaaat gaacaaagaa gcccgacgct tgggcatgaa
     5401 gaacactgta ttcaaaaacc cgacaggctt gagtagagaa ggacaggttt ccaccgccaa
     5461 agacctcgcc ctgctgtctg aagcattgat gcgcgacttt ccggaatatt acccgctgtt
     5521 ttccatcaaa tctttcaaat tcaaaaatat agaacaaaac aaccgcaata tccttttata
     5581 tagggacaac aatgtaaacg gtctgaaagc cggacacaca gaaagcggcg gctacaacct
     5641 tgccgtgtca tactccggca acggcaggca catccttgtc atcacattgg gttcggaatc
     5701 ggcggaaaca cgcgcatcag acaacagcaa gctgctgaac tgggcattgc aggccttcga
     5761 tacgcccaaa atatatccga aaggcaaaac cgttgcccaa atccaaattt ccggaggcag
     5821 caaaaaaacc gtccgcgcag gcttcctcaa agaagcctac atcactctgc cacataagga
     5881 agcgaaaatg gcagaacaaa ttctagaaac catacagccg attcccgccc cagtaaaaaa
     5941 agggcaaatt ttaggaaaaa tcaaaatcag acaaaacgga tacaccattg ccgaaaaaga
     6001 aatcgtcgca ctggaaaatg taaaaaaaag aagccggtgg caaaggcttt gggcgtgtct
     6061 gacagggcag taatctgccg tttcaaatat cccgtttttc caacaaataa agaaatgccg
     6121 tctgaaacac ggttcagacg gcataaaaca acagggcggt acgtattgca tacgcgccgc
     6181 cctgctgctg aaatcaatta gcgtttctta ccggtaacgg tagcaacagc cagattttcg
     6241 ttacgtttca gggaaacgct ttctacacct tcaggcagtt tgatgtctga caagtgcaga
     6301 atgtcgccgg caaccacttc agcacaatcc aaatccaaga aagcagggat gttggcaggc
     6361 aaagcaacta cttcaacaga agtgtttaac agagatacgc ggccgccttg cagtttgacc
     6421 gcttgggaat tttcagcgtt aacgatgtgc aggggaacac ggatgcgtac aagttgatcg
     6481 gctttcacag cttggaagtc gatgtgttga acttcgcggc ggaacgggtg catttggaaa
     6541 tcacggacga taacgtcttt ggtttcaccg ttcagagaca acttaatcaa cgcagtatgg
     6601 aaagattctt tttccaatgc gtagaatacg gttttgtgat ccacagcgat tgcaacaggc
     6661 tcttgacctt caccgtacag aatgccgggg atttggcctt cgcgacgcag gcggcggctc
     6721 gcaccagtgc cttgtgcttc acgaacagag gcttgaattt cataagtcat gttaaatact
     6781 ccaagttagg taaaatcgcc gtcatcggcc gcgaccagct taagacggct tcgggcttat
     6841 ggcagcaaca tgctgcctgt catcacttct tcattgaaaa gatatgagac ggattcttca
     6901 ttgctaatgc ggcggacggt ttcggccaac agaccggcaa tcgttacctg acggatacgg
     6961 tcgcagtttt tagccgcttc agacaaagga atggtatcgg ttacgaccac ctggtcgatt
     7021 tcggatgagg cgatacggct gaccgcctct ccggagaata cggcgtggct ggcataggct
     7081 agaacacgtt cagccccccg ctctttcagg gcgacggcgg ctttgcacag cgtatttgca
     7141 gtgtcaatca tatcgtccac aatcagacag gttctacctt gaatatcgcc gatgatgttc
     7201 atgacttccg ccacattggc tttcgggcgg cgtttgtcga tgattgccaa gtcggcattc
     7261 agggattttg ccacggcgcg ggcgcggacg acaccgccga tgtccgggct gacgacggtc
     7321 agattttcaa tccgctgttg tttgatgtcg ttcaacagaa tcggggtggc ataaatattg
     7381 tccaccggaa tatcgaagaa accttgaatc tggtcggcat gcaaatcgac agtcaaaaca
     7441 cggtcgatcc ctgccgaata cagcatattt gccaccagtt tggcagaaat cggaacgcgg
     7501 acggaacgcg gacggcggtc ttggcgcgca tagccgaaat acggaatggc tgtggtaata
     7561 cgacctgccg aagcacgctt cagtgcatcc gccatcgtca ggatttccat caggttgtca
     7621 ttggtcggcg cacaggtcgg ctgaaggatg aaaacatcgc gcccgcgtac gttttccaac
     7681 agttcgacgg caacttcgcc gtctgaaaac ttggatacgg aagcattgcc caaagaaatg
     7741 tccaaatgcc tgacaacacg ttgtgccaat tcgggattgg cattgcctgt aaataccatc
     7801 aaactgtcgt acgcagccat attctcacct gattttatgt ttaacttccg ctcagaaaac
     7861 acaatgcttc cctctgcttg taccaagccc gacattatac ctgcaacatc cgacacaaac
     7921 aaaggcattt caatattttt atttctatga aataaaagcg tgtaagcagg cttacacgct
     7981 tttatttggc tggggaggaa ggattcgaac cttcgcatgc tggaatcaaa atccagtgtc
     8041 ttaaccgctt gacgactccc caaaaagggc tggctgggga ggaaggattc gaaccttcgc
     8101 atgctggaat caaaatccag tgtcttaacc gcttgacgac tccccaactc gtttgacttg
     8161 gctggggagg aaggattcga accttcgcat gctggaatca aaatccagtg tcttaaccgc
     8221 ttgacgactc cccaacaatg tttatacgga caaccaagga tgttttgaaa gaccctctgc
     8281 caaatatgcc tcgtacaaat ctgaaacttg tcggtatata ttgtatgcgc tatccctatc
     8341 ttgacacgcc gtgaatacac acgcaccaga acctgtcatt aaggcaaatc catatcggga
     8401 caattcggaa taggctttcc aaacttcagg gtattcttta aatacaactg cctgcatatc
     8461 atttctaaac ggttgcaggt tttggaaagt cggcattatg cttgaggcgg aatttcgtgt
     8521 caagccttcg tgtgtgaaaa tttttgcagt ggaaacgtgg acgggcggtt tgacgatgac
     8581 ataccactgt ttcggaatat ccattcctat cagtttttca cctatcccgc ttgcaaaagc
     8641 attttttccg aaaataaaaa atggtacgtc cgcacccaaa gttgcgccca agtcaatcag
     8701 ctgctgcgtc gttcgggaac actgccacca acggttcaac accagcaaaa cggttgccgc
     8761 atccgagctt ccgccgccca aacccgcccc tgtcgggatt tttttgtcca gccatatttc
     8821 cacgccggcg gggttgcgcg catatttttg cagcaacgat gcggcacggt agcttaaatc
     8881 tacttcctgc ggcattccat caaccggatt gtgcaggatg attttgccgt cgtcccttag
     8941 tttcaaatat acggtatcct gcaaatctat caggcagaat atgctttcga tattgtgata
     9001 accgtcttcc cgctttccag taatcctcaa atcgagattc agttttgcag gtgcgggaaa
     9061 cgcctgccgt ccgtccgcaa tattcatctg tccgccttat ctcgtgcgcg ccgcacagcg
     9121 ttccagggtt tcagtttcag acggcatacc gatttcggtg aaaaccagcc tgatgttcaa
     9181 atttccgtta ttcagttgca acgttcggac ttgcccccca ctgtcggcgg ttctgccgac
     9241 agtccaaccg tattgttcca atatgccgtc cggcaggatg cggtaaggcg cgcccgccac
     9301 acgcctgcca tctgcccaga tatgcagata ttggattggc agtttgaaac cgaccagctg
     9361 cctgctcaat tcttccgcac tttccgcctg atagacattt cctttgccgt ccactgccaa
     9421 tgcgccgtcc ctgtcttgac acaactgccc gagcgtactg cccaaagggg tattgatatt
     9481 gatggtttcc acgggcggtt ggtatgtcca atcgaaattt gcatacgaac ctttcccttc
     9541 cgctttcact gccaaccgcc cttctgctgc aaaactgctg atgtgttcgg acggctgcca
     9601 caggttttcg ttattttgag gtaattgcgc gcaagcggtc aaaagcagga tgaccgatgc
     9661 ggatacggtg tgtttcatca gaatttcctt aacgggtgcc atcctgccgg ccggcgggtt
     9721 cggcgtgccg tgcaaaacgc gtgcgaactg cgtgccgttt gcccgaatgc cgcagcttcc
     9781 cggcaaacct tatgccgtct gaaaggatgg acctgcatta tttccggggt ttgcgggaag
     9841 gttggggcaa tgcgatgccg tgacgtttga gcgtttcccg ccatattttc ttgtctcccg
     9901 taaggtgtgc cgcctgcgtc catacgtcaa ccgcctgatc gcgttcgccc aatgcccaca
     9961 acacttcgcc caaatgggcg gcaacttcgg gctcggggtc gttttcaaac gaataccgca
    10021 gatacggcag cgcgctttcc gcgtcgcctt tcaggtaata cgcccagcct atgctgtcgt
    10081 tgacagcggt atcgtccggg ttgatttggt atgccgtctg aagcagggcg aagccttcgt
    10141 ccaaacgttt ggaatcggaa agcaggctgt agcccagatt attcataatc tgagcgttat
    10201 cgggtgcaag cctgaacgcc ctttcaagat ctgaaatcat ttttttccgc ttgccaagcc
    10261 gatcgtaaac aactgaccgc tgtaccaatg cctctgcctg taactctgta ttactgccgg
    10321 caggcggttt ttcgataatc ttgtccaacc ccctcaaagc ctcccgttta tcgggcagct
    10381 tcgacagggc gaacatctgt attttggaca aattgtctgc cgtaaaatac cgcccctgct
    10441 gttcgggaag tttccgcacc ctgccgatct gccgcaaagc cgccctgccg ccgtccaact
    10501 cgacagccgc cgcagccgcc agcacacctt tgtcgaacag gtattccggc gcggacactt
    10561 ttttcaacca ctgcctgact ttggcgtaat ccctgcggtc ggcatacatc atcgccgccg
    10621 ttagcgccgc cctgctccgc tgttcctccg tccccctgcc gtatgccttt tcggcgtagc
    10681 cgtcgataac ggaagcacct tcttttcggt ttgccgccaa tatcgctgcc tgaatataca
    10741 ggtctgcatt cggattgcgt tccaacagca cgttcaaacg cgcataggca tcatccagcc
    10801 tgtgcaggga aaccagattc ataatttcca tttcctgcca gacggccgaa aggttttggg
    10861 tgtctgtctg ctcgaaaaag ccgtcgagta tttcgggata tttgcgtgca gtcagacgca
    10921 acgtcattaa agtggggggc aatatttccg tatcgagctt cgccaaacgc tgcaaagctc
    10981 cgattgcctt ttccttttcg cgtccctgta cgctgaacac cacatcggca accgccgctt
    11041 cgggcagatg ttcatatttc aacgccgcgc ggcgaaccgc tttcgatgct ttttgcgcca
    11101 acccgtcctg ttgcacggcg gcttgtgcca acaataaaaa caccctgcgg ttctgtcctt
    11161 cgtccgcctg agccagcact tcttccagtc cgtccagatg ctgatttcct ctttccctca
    11221 gcacgttccg cagccacccc gcccgttttt gcgccttacc cggtataggc tcaatctgcc
    11281 gccatttctg ataaatcatt tccgcctgtt caaacgcgtt cagcgacacg gccatttcca
    11341 aggcgcgttc ggcgacttcg ggggattttg tgcgttccaa catcagcata taggttgcca
    11401 gagccgttcc cgcctgcccc ttttgcaagg cggtttcccc tcccagcaac gtaaatatct
    11461 gattaacccg ctcgcccact gccgcaagcc gtgcgcgttc gtttttgatt tcttcctcgc
    11521 tgtaacgctg ctgctttctg aaaacctttc cgacttcctt cggcggcttc gcatccgccg
    11581 cgccggcggc atacgcctgc ccggcaagca gggctgccgc gagcacagat aaaatggtga
    11641 aacgggcggg taacataatc ttcctttatc cgacagccgt ccgaaccttc agacggcata
    11701 taatcaggcg taactttatc acaagcagaa tcgtttgtag atttgcgaaa ccccgttcaa
    11761 aaaaataaat gccgtctgaa acgcaaatcc gcttcagacg gcattcccga cgcttgcggt
    11821 ttaagggagt tttatttcca aattgtcaat caggcgcgtc gtccccagac gggcggcggc
    11881 caagaccacc agtttcttat ctcccgcccg cgccacttcg agcgtatcgg cgcggcggat
    11941 ttcgacataa tcgaccaccc agcccgcagc ggtcaaatgg tcggcagcct gccgctccaa
    12001 acctgcataa tccaaactgc cctgcgccaa ggattcggca acagccttta attcgcggta
    12061 caggcgcggt gcttcgtcgc gttccgccgc actcaaatac tggttgcggc tggagagtgc
    12121 caaaccgtct tccgcgcgcc ccgtatcgac aggcactatt tcaacatcaa aattcaaatc
    12181 ctcggtcaaa cctttaatca cggcaagctg ctgatagtct tttttgccga aacaggcaac
    12241 atccggcgcg acaatattga acagcttgga caccaccgtc gccaccccgc ggaaatgtcc
    12301 cggacgaaac ttgccgcaca actcattttg caggttgggc ggttcgacgt tataccgttg
    12361 ctccacgttc ggatagagtt ctttttcatc gggcgcgaaa acaacggcaa caccctcggc
    12421 ttccagcttg tccgcatcct gttgcaaagt acgcggatat ttgtcgaaat cctcgccctg
    12481 accgaattgc aggcggttga cgaaaatgct gaccacgaca ctgtccgcgc gttttttcgc
    12541 ctcacgcaca agctcaagat gcccttcgtg cagattgccc atagtcggca cgaacgccac
    12601 cgttcccgca tttttacgcc acgcgcgcag ttctcgaaca gtatgtatga tttgcataac
    12661 ggtaatcctt aatccttcgg gcttcctgcc cgacaaaacg cgccgattat acgcgccgcg
    12721 cacggcaaaa caaaatgccg tctgaaacgg ctttcagacg gcatcggcag caaaccttgt
    12781 tcagtctgca aaaatatgtt ccgcagcagg gaaagttttg gctttgactt cggcaacata
    12841 cgcccgaacc gccgcctgaa tgctgctttg cccgtgcata aagtttttga cgaatttcgc
    12901 cgtcttgccc ggaaaaatac cgagcatatc gtgcatcacc aaaacctgcc cgtcgcaatc
    12961 cgcgcccgca ccgatgccga tagtcggaca ggaaacagtt tcagttacct tttttgccag
    13021 ttccgccagt acgcactcca tcagcacgac cgccgccccc gcatcgtcat gcgccttggc
    13081 atcgttaagc aacgcctgcg ccttgccgcc gcgcccctga actttatatc cgccgaaggc
    13141 aaacacggat tgcggggtca gaccgatgtg cgcacaaacc ggaataccgc gcatttgcaa
    13201 aaattcagtc gtttccgcca tccacacgcc gccttcgagt ttaaccatat gcgcgccggc
    13261 agccatcagt tcggcggcgg cggcaaacgc ctgctcctta ctctgctgat atgcaccaaa
    13321 cggcaaatcg ctgacaatca tcgcattttt tgcaccgcgt gctacacatt cggtgtgata
    13381 acacatatcg cgcaggctga cgggcagcgt cgatttccgc ccctgaaccg ccatccccaa
    13441 agaatcgccg accagcagca tttccacgcc ggcatcgtcc atcagcgcgg caaaactgga
    13501 ttcgtaagcg gtcagcatag cgattttttc gcccgccgcc ttcatttttt gcagtgtgtt
    13561 cacagtaatc atcagatatt gttaccccgc ccttttcaga cggccttttc agtttgaaac
    13621 gggctattat agtgaaatgc ccgcagccct gccaccgacc gtgccgcaaa tgcaaaacag
    13681 cccgactgtt ttacacaatc ggactgtcaa atctggtgcc ggcaccaaga gtcgaactcg
    13741 ggaccccctg attacaagtc aggtgctcta ccaactgagc tataccggct tactgaaatg
    13801 ttttgcaaca ccatcagcag aaatggtgcc ggcaccaaga gtcgaactcg ggaccccctg
    13861 attacaagtc aggtgctcta ccaactgagc tataccggca aagaaagcga attatgcagg
    13921 caatgccgca ccttggcaag gaatttatcc gaaagccccg gcaaatccga ataagtaatt
    13981 tatttgaaaa gaaattaatt tgaaacagaa caagcatcgg cattctgata aaatatccgc
    14041 ctttcaaacc gaccgttcac accgatggca agacatccct accgccgcct gcgtcctgca
    14101 aaatccggct ttcccgaagt cggcatttcc gaagaaggca atatccgttc gcttcacttg
    14161 ggcagcgaca ccgttcaaag ctcgatgaac ctcgaccacc cgtccgagct ggtgctctct
    14221 tacagccgcg cgatgatggg ctggctgctg tttaccgacg ctcttccgca acatatcacc
    14281 caaatcggct tgggcggcgg ctcgtttgca cgctggatag atacctacct gcccgacacg
    14341 cgccaaaccg ccgtggacat caatccgcag gtcatcgcca ttgcccgcaa cctgttcgag
    14401 ttgcctttcg agggcgagaa atttgaaatt attgaagcag acggtgcaga atatatcaaa
    14461 gtcttccgcc acaacaccga tgtgattttg gtggacgggt tcgacggcga acaaatcatc
    14521 gatgcgctgg ttgaagaacc gttcttccga gactgccgca acgcactctc ttcagacggc
    14581 atattcgtaa ccaactggtg gagcggcgac aaacgctacc aacgcttcat cgaacggttg
    14641 ttgagcgttt ttgaagggcg cgtcttggaa cttcctgccg aaagccacgg caatgtcgcg
    14701 gtaatggcct tccaaagcag ccccaaagag caaaacatag acaaactcaa aaaacgtgcc
    14761 gacaaactga gcaacgcata cggattggac ttccaccgta tgcttgccgg cctgaaagcg
    14821 tccaacccca acaacggcaa gcatttccac ctttaagcaa ctatcttcac ggctgcatcg
    14881 gaacatgcgg aaaatcaaga ggaaatcatt atgctgaaac ccgaaaccat acacatatcc
    14941 ggtcctgccg gcattttaga aaccatccat atcccgtccg aacaagtacc ggcacgcggt
    15001 gttgccgtca tcaatcatcc caaccccctc cagggcggaa caaacaccaa caaagtcatc
    15061 caaactgccg ccaaagcctt aagcaaactc ggcttccact gctacctgcc caaccttcgc
    15121 ggcgtaggcg gcagcggagg cacacatgat tacggacgcg gcgaaacgca agactgcctc
    15181 gccgtcatcg attatgcccg cgcccaacac cccgaagccc ccgaatttgc cttatccggc
    15241 ttctccttcg gcggttatgt cgccacattt gccgcacaag cgcgcacacc cgatttattg
    15301 ctgctcatcg gcgcggcagt ctgccactat accgaccgtc cggaaccgtc cgccgttccc
    15361 aacgttgcca aaacgctgat gatacacggc gcggaagacg aagtcgtcga aatcgggaaa
    15421 gcactgaaat gggcggaacc gcaagatttg cccgtcatta ccatcgccgg ttccacgcat
    15481 ttctttcacg gcaaactcat cgtcctgcgc gacaccatcc tccgcttcgc gcccgtatgc
    15541 ctgaacggat aaaacccctt tcagacggca tcgataatgt aaaacccgta tcggcggcat
    15601 ccgccgatac gggtttgcgt tccctctcat tataggcaaa atccgctgca aaacaccctt
    15661 tcccgacccg acaaaggatc tgcaaacgca atcttttttg ccaatagctt caaaggtttc
    15721 cgataatcct cgctgcccgc ttcagacggc acgggataga gcgcgtcatt caacagcggc
    15781 atacccaaac ccatcatatg cacgcgcaac tggtgtttct tgcccgtatg cggcgtaagg
    15841 cggtaaaggc tgaattcccc cctgttttca atcagttcga ccgtcgtatg tgcgtttggt
    15901 tcgccttccg cctcttgcgt cgtaaaaaat ttctcacccc tcaccaaacg cgaaaccaca
    15961 tcgagcggat acggcaaatc cgtccttgtc ggcgcaagcg cctcatacgt tttccatacc
    16021 gttttgtttt gaaacatcgt ctgataggct ccgcgcgtgg caggattgtg cgacagcagc
    16081 atcacgcctg ccgtatcctt gtccaagcgg tgcagcggcg taatgtcctc aacattcaaa
    16141 tgctgcaatt caggccgcaa acgcaggcgc gtgagcaggg tttcccgcaa aaacctgccg
    16201 ctggggatga cgggcagaaa atgcggtttg tccaccacaa tcaaatgctc atcaatatgc
    16261 aaaatctttt cttcaaacgg aatacgcggc tcgctctcac ggctggtttc acggtaataa
    16321 aacatcacct tacccggctc gaacaaagaa tgttcgtcca acgccgcacc atccgaaccg
    16381 accacaaaac cgctgttcaa ccgcctgcgc caatcgtccg cgcccacaaa aggaaagcgg
    16441 atgcacagaa aatgcagcag cggcagcccg taaaactgct tttcatgcgg cagcaccaaa
    16501 taactgggtt tgacaccgtt caacagcgga agaggattat tgcgtttttt catgcggcgg
    16561 attgtaaccg tttttcacaa acttttcaga cggcatatcg aaacataatt gacaaaaaca
    16621 aacatattgt tttttatgat ttaaaaattt atttagacaa gattatcgga tattcattat
    16681 tatcagtaaa taagaattaa tatcaatagg agaaatatga agcgcatctt tttgcccgcc
    16741 ttgcccgcca tcctgccttt atccacttat gccgacctgc ccttgacgat tgaagacata
    16801 atgaccgaca agggaaaatg gaaactggaa acttccctta cctacctgaa cagcgaaaac
    16861 aaccgcgccg aacttgccgc accggtttac attcaaaccg gcgcaacctc gtttatcccc
    16921 attccgaccg aaatccaaga aaacggcagc aataccgata tgctcgtcgg cacgctcggt
    16981 ttgcgctacg gactgaccgg gaataccgac atttacggca gcggcagcta tctgtggcac
    17041 gaagaacgca aactcgacgg caacggcaaa acccgcaaca aacggatgtc cgacgtatcc
    17101 ctcggcatca gccacacttt ccttaaagac gacaaaaacc ccgccctaat cagctttctt
    17161 gaaagcacgg tttacgaaaa atcgcgcaac aaagcctcgt cgggaaaatc ctggctcatc
    17221 ggcgccacca cctacaaagc catagatccg attgtccttt ccctcaccgc cgcctaccgc
    17281 atcaacggca gcaaaaccct ttcagacggc atccgctaca aatcgggcaa ctacctgctg
    17341 ctcaacccca acatctcatt tgccgccaac gacagaatca gcctgaccgg aggcatccaa
    17401 tggctgggca cacagcccga ccgcatagac ggcaaaaaag aaaccgcaag aaacacatcc
    17461 acctacgctc atttcggcac aggtttcggt ttcaccaaaa ccacggcttt aaacgcatcc
    17521 gcacgtttca acgtttcagg gcaaagcagt tccgaactga aatttggcgt acagcataca
    17581 ttttaagcag gttttgattt tccaaccttg taatccaaag gagttattat gaaaaaacaa
    17641 atcagctcag ccgtaatgat gctgtctatg attgcccccg caatggcaaa cggcttggac
    17701 aatcagacat ttgaaaacca agtgttccac acgcaggcag atgcgccgat gcagttggcg
    17761 gagctttctc aaaaggagat gaaggagact gagggggagt ggattcctaa tgctgttgga
    17821 ggcattatgg gaggagttgg agggcatttc agctatatgg ctagtgctat tgctagtggc
    17881 tcgtataatc gaaatgaaca ttggacagca attggaggtg gtgcgttaat tggtgcgatg
    17941 aaccctatta acggaggaag agcactgatt aatggtatgc gaggtgtagc agtggggact
    18001 gttgctggtg gcataaatgg ttatgcaggt tctacgggta agaacactga cattcgccgt
    18061 taaataataa ataaaccaac ttccaaccaa acctgctatc ggtttggttg gaag
//
LOCUS       DAWISC010000022        18006 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWISC010000022 DAWISC010000000
VERSION     DAWISC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.022"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..129)
                     /locus_tag="WIW87_000531"
     CDS             complement(<1..129)
                     /locus_tag="WIW87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEZ5173730.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAE"
     gene            374..1282
                     /gene="hslO"
                     /locus_tag="WIW87_000532"
     CDS             374..1282
                     /gene="hslO"
                     /locus_tag="WIW87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ5173731.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLVNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WIW87_000533"
     CDS             complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WIW87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ5173732.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(3054..7346)
                     /gene="mspA"
                     /locus_tag="WIW87_000534"
                     /gene_synonym="ausI"
     CDS             complement(3054..7346)
                     /gene="mspA"
                     /locus_tag="WIW87_000534"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ5173733.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFTVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPTALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGNSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMNLN
                     GEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAGRN
                     TAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPATQV
                     RGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQIT
                     LNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPLKLEGDSRGAFQIHVK
                     NTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPLKE
                     AELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARIDSQ
                     VQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELDTYI
                     ERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLAEL
                     ISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRPYQ
                     QTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGALF
                     AAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNGNR
                     YVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSVND
                     RTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            complement(7660..8418)
                     /gene="gloB"
                     /locus_tag="WIW87_000535"
     CDS             complement(7660..8418)
                     /gene="gloB"
                     /locus_tag="WIW87_000535"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ5173734.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(8513..12475)
                     /gene="purL"
                     /locus_tag="WIW87_000536"
     CDS             complement(8513..12475)
                     /gene="purL"
                     /locus_tag="WIW87_000536"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ5173735.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSGVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDARRGAVFKLREVPLEEHGLNPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKLPKTTRTDKTVAPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGLTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYSVIQQLVAENKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            12626..12964
                     /locus_tag="WIW87_000537"
     CDS             12626..12964
                     /locus_tag="WIW87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ5173736.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            complement(13030..14118)
                     /locus_tag="WIW87_000538"
     CDS             complement(13030..14118)
                     /locus_tag="WIW87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NadA"
                     /protein_id="HEZ5173737.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATSDDDVKKAATVAIVAAYNN
                     GQEINGFKAGETIYDIGEDGTITQKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDETTNALNKLGENITTFAEETKTN
                     IVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVD
                     AKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKADIAKNSARIDSL
                     DKNVANLRKETRQGLAEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTE
                     NFAAKAGVAVGTSSGSSAAYHVGVNYEW"
     gene            complement(14406..15164)
                     /locus_tag="WIW87_000539"
     CDS             complement(14406..15164)
                     /locus_tag="WIW87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5173738.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADYVVAMKNGQVAMQG
                     KPNDIFTAENIKTLFDMDVNVLDYEGKKLVIHHI"
     gene            complement(15249..15875)
                     /locus_tag="WIW87_000540"
     CDS             complement(15249..15875)
                     /locus_tag="WIW87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173739.1"
                     /translation="MTQEHFPSFFNQAPTITVRDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            complement(15920..16894)
                     /locus_tag="WIW87_000541"
     CDS             complement(15920..16894)
                     /locus_tag="WIW87_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ5173740.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYTSLPAVGKFGFELAVMMGGSLLLFYTLVKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFASFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            complement(16884..17852)
                     /locus_tag="WIW87_000542"
     CDS             complement(16884..17852)
                     /locus_tag="WIW87_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5173741.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
BASE COUNT         3633 a         4486 c         5461 g         4426 t
ORIGIN      
        1 ttcggcaaac tgtctgagaa tctgctcgcg gctgctgagc aggttggtca actcgtcggc
       61 aagggcgggg cggacggcaa acatcagcca caaaaccgcc caaactgccg gtttgaaaaa
      121 agaatccata tcggtgtttc cgcgcaggag gcatcgtgcc gccgtgcgat gttatgctgt
      181 atcagtcgaa aatatcttgt tgattgtata taaaaaaacg gctgtttggc aaaaaacggc
      241 ttgttagatt gagttaatat gttgattttt atataattta ttgataattt gggaagcgca
      301 tccgccgtcc gtcttgtttt cgcgccgacc gcaaccatat agccgccatc cgaacaatca
      361 gtcagagaaa atcatgaatc aaaccgccat caaccgcgcc gatgtgcgta cccgctttat
      421 cttcgacgat atgcccgtgc gcgggctgca cgtccgtctg gaaaacgtgt ggcagcacat
      481 tgtaaaacag aaaaactatc ccgccgccat ccgccgcgcc ttgggcgagc tgttggctgc
      541 cggtgttctg ctgtcgggca acctcaaaaa cgaaggcacg ctgattgtgc aggttcaggg
      601 gcaggggcgg ctgaaaatgc tggttgcgga agcgacttcc gaccgtaccg tccgtgcgac
      661 cgcgcggtgg gacgaaaccg cagaaatagc cgatgacgaa agcctcggcg atcttttggg
      721 cgagggcggc gtattcgtgc tgacgctgca gcctaaagac ggcgaaccct ggcagggtgt
      781 agtgcctttg gaaggcgacg gtatcgcgca aatgttggtg aactatatga aacgttccga
      841 acagcttgat acgcacatcg tcctgtctgc aagcgacgaa gcggcgggcg gtctgctggt
      901 gcagcgtctg cctgaagagg tattggatga agaggcatgg gaacacgtca gtacgctggc
      961 gcgcacgctg acggcggagg agctggcagg actggacgcg caacacgttt tataccgcct
     1021 gttccacgaa acgccgccgc gcgtgttcga gccggaaacg tttgaatttt catgcacctg
     1081 ttcgcgcggc aaagtcagcg atatgctgtt gatgctgggc ggggaagaag tcggcggcgt
     1141 ggtggtggaa caaggcagca tcgaagtcga ttgcgatttc tgccacagca aatatgtgtt
     1201 tgacgaaacc gatgtcaacg cgctgttcgg ggaggatgtg gtcggcgttg ccaaagggct
     1261 gccccggcat accgtccaat aatcttgtgc aacaaggata aatagtcaaa tgccgtctga
     1321 agcagcttcc gcttcagacg gcatttttgc gcgggttcag acggcattta aagcaggaat
     1381 agggtggcga gccccaagaa aatcaggaag ccgccggagt cggtaacggc ggtaatcagc
     1441 accgagctgc ccagtgcggg atcgcgtccg aacttttcca ttaccacggg aattaatacg
     1501 ccgacggttg ccgccagcag gaggttgagc gtcatcgcgg caatcataac cagcccgatg
     1561 ccgaggctgc cgtaaagcag ccaagatact gcgcccatga ccgttcccca aatgatgccg
     1621 ttgaccaagg cgacaccgac ttcttttttc agcaaacgcc ccgcctgcat atccgtcagc
     1681 tgccccatcg ccatcgcgcg gacaatcatg gtaatcgtct ggttgcccga gttaccgcct
     1741 atgccggcga cgatgggcat cagcgcggcg agtgcgacga ttttttcgat gctgccttca
     1801 aacgcgccga taacacggct ggcgaggaag gcggtgcaga ggttgacggc gagccacatc
     1861 cagcggtttt tcaccgaatc ccacacgggg gcgaacaggt cttcctcttc ctgcaaaccc
     1921 gccatattca gcatatccgc ttccgattct tcgcggatca cgtccaccat ctcgtcgatg
     1981 gtaatcctgc cgatgagctt tttgttttca tcgacgacgg gcgcggtaac caagtcgtag
     2041 cgttcaaacg cctgcgccgc ttcttccacg tcatcttcgg cgcggaaacg cacgacatct
     2101 ttcgccatca cgttttccac caagtcttcg ggatcggcga ccaaaagttt gcggatgggc
     2161 agcacgccct gcagtacgtc gttttcatcg accacaaaaa tcttgtcggt atggtcgggc
     2221 aggctgtcga agcggcgcag atagcgcagc accacttcac aggcgacatc ggcgcggatg
     2281 ctgaccaact cgaagtccat aatcgcaccg acttggttgt cttcgtagga cattgccgct
     2341 ttgacttggg cgcgttcttc ctcatcgcgg gtttgtagcg cttcgtaaac cacttggtgc
     2401 ggcaaatcgt ctgccagttc cgccaattcg tccgcgtcca aatcatcgac cgctgccaac
     2461 aattcgtctt tgtccatcga ctcgatcagc gtttcgcgca ccgcgtcgga tacttccagc
     2521 aatacttcgc cgtcgtcttc cggtttgacc agaatccaga cgatattgcg ttcgcgcggc
     2581 ggtagcgatt ccaataccgc cgccacgtcg gcagggtgca gctcgctcaa gaggacggtc
     2641 agctcggtca gcttgtcgcg cagcggcgcg tcttcgagcg gtgtaccgtt ttcgatttgt
     2701 tcaaaagcag gttcgaggat ttcgcagagg gagtggacac ggtcgaaatc ggcggaaacg
     2761 cgttctacat cgttttcgat accgtcgttt tcaaggttcg gaggggttgg ttcgatgctc
     2821 ataaatgctc cgcccgccgt gtgcggggag gcattccggc gggatggtta ttgcgggttt
     2881 gaatcggatg gggtgttcag taaaaacgaa ctgggaaggt ccataataaa agcctgacgg
     2941 tacaggcgca gggttggaaa cggcactatt ctactccctt ttggaacggt ttactatttt
     3001 taacgcaaat gccgtctgaa acgcgggttc cagacggcat ttttatcggc ttgttaccag
     3061 ttgtagccta ttttgattcc tgcctgtttg tggcgggcgg tgttgctgtc tttgccgtaa
     3121 gcggcatgaa gtttggcgtt ccagcctttg tatccggcgt cgatttgcag ggtatgcagt
     3181 gtgccgtggg cggcttgctg cagtaaggta cggtcgttga cgctgagggc ggaaccgctg
     3241 ttttggcggg tatggtagta atcgctgctg aaggcggggg tcagcttggc ttgacccagt
     3301 tcgacggttt tatcgagacg gatgccggca tgccatgtgg tttggatttg cgccgggctg
     3361 tttatctctg cgccgtcgag tacgtaccgg ttgccgttgc tgcggtttat acggatgccg
     3421 gcatagggtc tgaggttgat gccggtatcg attttgatgc cggtgttgat gcctgcatcc
     3481 catgcgtggc ggcggacggc agccccgtca taatcggtaa atcgggtacg gctgttgctg
     3541 tagcctaaat ctgccgcggc aaagagtgcg ccgttttccc ctttgacgaa cagatgtgcg
     3601 ccgttgctgc ggtttcgggc ggatacgcct tcatcaaaac ggttgtttgt gcgctcatcg
     3661 gttaaaatcg taccgacact gagacggtcg gtgatgccgg tttggatgcc gatatgtgca
     3721 tagttggtag tttgttggta gggacggtgt gtgccgctat ggtagtcggt ttgttgcgta
     3781 ccggtttcca gccagatgtt ttgctgctgc ggatcggtaa ggtggcggtc gatgcggcgt
     3841 cccgcctgtt gccggccggt attgtaggcg gcctgttcgg aaacggcggt gttggccgac
     3901 cggctgatca gttcggccag gttcggcata cggatgccgg caacgccggt tttcaaactg
     3961 tggatttgtc gggacagacg gttgagtgcg ttcaggtagg cgtgccgggc tgtttcgacg
     4021 gcttgcgcat cgccgccttg ccgtgctttg tcaagttcgg attcggccat ttctacacgt
     4081 tctatatacg tatccagttc ggtttcgaag agtgtcaggt cgttcgtgtc ggcggctttg
     4141 gcaacctgac ggcagatatc gacactgtat ccttgtgcgg cacacaggtt ttgggcacgc
     4201 gtcagaatgc cggtcagcgg gtctgtctgt ttcaattggg cggacagata ttggacttgg
     4261 ctgtcgatgc gggcaagttc ggtttgactg ttctgccagg cctgtagtgc ctgcctggtc
     4321 gcgtcagagt catgttgaac ctgtctgctg atgtcggttg cctgtaattg gttgtatgcc
     4381 tgttggttgc gctcatgttc cgcacgcgtg gcttcaattt gaagttcggc ctctttgagc
     4441 gggttgtaca ggctgtatcc gttgttgttt ttgcggagga tgtagcggta ggcacccaaa
     4501 tcggcatagc cgttttggag ggtgaatcgg gcttggtggc tgtgtttcgg attgaggctc
     4561 acaagtgcaa gcgattcggt tgtttgaggt tcttgtccgg tgtttttgac gtggatttgg
     4621 aatgcgccgc ggctgtcccc ttccagtttg aggggggcat tttgttttcg gacgatgccg
     4681 gtcaggaatc ggaatgtgcc gaacccgtca agtgtgccgt tgacggtcag tgtgttgaag
     4741 cggttgttgt gtgtattatt ggcgaaatcg gggttcaggg taatttgtgc gccgtcaagc
     4801 gtcagtgcgc cggtgtggct ggactgggaa agtgtccagt tgctgtctgc ttccatgcgg
     4861 actgcggtgt ctttgccggc acggatgctg ccgtacaggt gtgctttgcc caggcggagc
     4921 tctgaacggt cgttaagggt aatgtcgccg cgtacttgcg ttgcaggtag tgcacgatag
     4981 ttttcggctt ttaaaacggc gttgggtgtg cagtgggtgc tgccgctatg gtaggagcgg
     5041 tagcattccg gtgttttgcc ttgggtaaag ccgaggtcga tgccggacag atcgtatgcg
     5101 gttatgtcgc cgtccagatg cgcggtattg cgccctgccg tcagtcgggc atggtttcgc
     5161 agggtgaacc gtgcagcctt gaagctgccg tcggtccatt cgttttcaag aacgggttcg
     5221 cgtttggcct ggtggtcgta ggcatggggt acgggcctgc ctgacacaat catattgccg
     5281 ccctcaatca agacttcccc gttaaggttc atgccgccgt tgagcaacag cgtgctgtcg
     5341 gtgcgtttgg gacgatagtg caggttgagt gcgccgttgt aacggtattc gggccggccg
     5401 tatacggcgg catttgcgat atggcgggtt ttttctatgg ctgcttcatt ggttttgctg
     5461 taactcggcg cggctttgcc cgtttgcgcg ttttcaccca agtatccgcc gaaggtaatc
     5521 aggtcggggc gggcattttc ggcttgggcg acttgttcgg cggcctgttg cctgttgttg
     5581 ccgataaatt gccagcttgt tgagtttttc atatttaacg ggaaaaattc gcgcgggttg
     5641 ccgccgggtt tgagtatgaa gtagtcggtc cgacggttgc ggtgcgggtt gatgtattcg
     5701 taaaccgccg cgttgccttg cggacggttg ccccattgca cccactcgac atgctcggga
     5761 ctgaggacgg ggttgccggt cagcgtcagt gtcgcggctt ggtcagggtt gtgattgacg
     5821 atttgcgcgc cgccgtccgc atggcggata tgggtaaagg caaggttgtt gccgttgagg
     5881 tcgagccgtc cgcccctgaa gccgaaatag aggttttcgg gtttgatttg ctggctgtcg
     5941 gcgaggacgg ccgtgcccct gccgctggtg atgccgactt ggttgaatgc ttgtttgctg
     6001 ccgtctgaag cagctttttg ggcgagtacg acagtgcctt ccccgatgct gatgtcgccc
     6061 tggttgatgc cttgtccgtt ggcgataagc gtgcctgcgc ccagtttgga gagccggtcg
     6121 cctttggggt tgctgacttg ccagaagacg cgtttgccgt cggctacgat aacgcctgca
     6181 ccttgccatg tgtggttttt accgacgacg gtgaagttgc tgtcgaactg caatgcgcct
     6241 gcgccttggt tgatattgtc tgccagcatc agtgttttgt tgtcgaacct gctggatagg
     6301 atgagcgttt tgccggcatc ttcagacggc atgtgcctgc tgtcgttttg tggggcaagc
     6361 gaagggtttg caatgggcag ggtgatgcgt tcgttgagcc cttgcagggt gctgttgcca
     6421 ttaccgttgt cgcgccatat gagttcgttg gtggtcagcc cgacatccag ccgggtttcg
     6481 tattggcgga tggtggaacg gatgaattcg ggttgcgtga cgatgtattt gttgaagaaa
     6541 ttatcgaagc cggcgtaggt gctgagtacg cccgcaagca cccagcggtt ttcatgcttg
     6601 tcgaaggcaa acagggggga accgctgtct ccggcgattg cgtaggtgtt aaggggttgg
     6661 tcggtcaggc tgccgccgac gagtaagccg tggttttgga accccaatac tttcagcggc
     6721 gtgccgccgg tcaggtattg gtatgccggg gcggttcgtg tacgcgtgcc gtctgctttg
     6781 cggacttgtt gcgtgcctga gccgagtcgt acaaagtagg ggaagcggtc ggtatcgagg
     6841 taggcattgg cctttggctg gccgtttcca agcaagggta cgctgctgag tgcggtaggt
     6901 gagatttcgg taaccagttt gttgaggcgg ggaaggtggt agtcgtagtc cgggtgcggg
     6961 ttgcgtgata cgaggcggta ggtataggct tgttcttctg gattttgggt gtcgttgccg
     7021 aattgtatgg aaccgtagcc gacgttgtgt ttgacactgt tgacgtattg ggggtgaacc
     7081 agggtggcga tggcggtttg gcggttgctg acgcggaagt cgggcatggg gatgccgttg
     7141 agaaccctgc ctaatatttt gccttgcttg tcttggatgg aaatattgct tgcacctacg
     7201 gtgaacgcgc ctttattttc ggcaaagtcg cgaaaatatt ggtaatcgac atcgttgcgg
     7261 acaatggatg agtaagcagg ggaaacggca aaaagaccga gggtggacaa tacggaacaa
     7321 aatggggtgg tgtgtgtgaa gcgcatgatg attatccgtg taatagagaa aatcaacgat
     7381 tcttgcggac aagaatcacc gatcccgttt tgtgctggat ttatatagtg gattaacaat
     7441 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tctgcggctt
     7501 cgtcgccttg tcctgattta aattcaatcc actatattat atattttgtt tttgtttgat
     7561 tataaggcaa cggtgtcctt tcgtctattg gcgatgattg tatgatttta tttaagattt
     7621 taataaaaat cctaggaatg gctgcgtttt cccgttgttt tatttcgtcc ggtattggtt
     7681 tttaagttca cgcagcgcga caaaggtatc gaggctgctg tttaacgttt tcccgctcaa
     7741 tgcctcggcg cggtctctga cgtgcggcag gtcgacgcgc aaaaacggat tgacgcggcg
     7801 ttcgtgcgcg agggtaacgg gcagggtagg cgtatgcgcc gccgctttca gtgccgtctg
     7861 aatgtcggcg ttgtccggct cgatatgggc ggcgaaacgc aggttggcgg cggtgtattc
     7921 gtgcgccgga tagaacaggg tgttttcagg caggcggttg aagcgttgga agctgtcgta
     7981 aagctgttcg attgtgccgg taaacacgcg tccgcagccg gcggaaaaaa gggtgtcgcc
     8041 gcaaaagacg tgtatgccgt ctgaagtttc gagaaggtag ctggtgtggc ggtctgtgtg
     8101 gccgggtgtt gcccaaacgg taacctgtcc gtcgccgaag gtgaattggg tgccggcggt
     8161 tacggtgtgg gttgctgctt cgatgtcgga ttcgccgtaa acgggcgatt ccatgtagcc
     8221 gcgccagagt gccgccgcac cgccctcgtg gtcgggatgg gggtgagtta cccatgtttg
     8281 ggcaagcatg aggcggttgc ggacgaggaa ttccaagacg ggcgagggtt cggaagggtc
     8341 gacgcagacg gcatggttgc cgtgctgtat catccagatg tagttgtcgg ttagggcttt
     8401 gacgggggtg attttcatga gtgttcctcg gtggacggat accgtttaaa ggatggtatg
     8461 ccggaacgcc gtctgaaata ttcttcagac ggcattggtt tgagtagggc ggttagccca
     8521 aggctttacg tgcgccggca aagaggcggt accagccgga caattccgtc cagtcttctg
     8581 gtttccagct catttgtgcg gcacggtaca cgcgttccgg atgcggcatc atgatggtca
     8641 cgcggccgtc ggcgttggta acgccggcga tgccttgcgg cgagccgttg gggttgagcg
     8701 ggtaggtttg ggtcacttgg ttttgaccgt ccacatattg cagcgcgatg cccaaatcgg
     8761 cggaaatatt gccgccgtga agcgcgaagt cggcgcggcc ttcgccgtgg ctgaccacga
     8821 caggcaggct ggagccttgc atttcgttca gaatcagcga cgctgatttc ggaacgtgaa
     8881 ccatgctcag gcgtgcttca aactgttcgc tcaggttgcg tttgaattta ggccatgcct
     8941 ctgcacccgg gataatctcg gcgaggttgc tgaccatctg gcagccgttg cacacgccca
     9001 atgtcagcgt gtccggatcg gcaaagaagg cggcaaattg gtcgcgcaag gcagggtgga
     9061 acaggatgga tttcgcccag ccttcgcccg cgccgagtac gtcgccgtag ctgaagccgc
     9121 cgcacgccgc cagcattttg aagtcggcaa ggcggaagcg gcctgccatc aggtcggaca
     9181 tatgcacgtc gtaagcatcg aatccggcgc gggtgaaggc ggcggccatt tcgatttgcc
     9241 cgttcacgcc ctgttcgcgc aggatggcga ttttgggttt cgcgccgctg ttgataaacg
     9301 gcgcggcgat gtcttcgttc acgtcgaact tcacgtcggc aaacaatgcg ctgcggtcgt
     9361 tgtcgccaat cagtgcgaac tcgctgtcgg cgcaggcagg gttgtcgcgc aggcgttgga
     9421 tttgatggct ggtttcttgc caggtttgtt gcagtttgat taggttgtca gaaataaggt
     9481 gcgtttgccc gtcgcggatg attaacgtgt tctcatcagt taacgtaccg atttcaaaga
     9541 cattatgatg cagctgttgt tgatagaata aattgataat atcggcaaca tcttgttttc
     9601 taacttggat aacagcaccc aactcttcat taaataatgt gcgggcaatg gtttcttgcc
     9661 attcagccaa cgcttttacc tcttcagtcc gcaatgattg agacagagcg gtatggttgg
     9721 taataaatgt ttgtgcaagc aataaattta aatctatatc caagccgcac cgccccgcaa
     9781 acgccatttc taccaaaacg gcaaacaagc cgccgtcgct gcggtcgtga tacgccaaga
     9841 gtttgttttc ggcaacaagc tgctgaatca cactgtaaaa ggctttcaga cgacctgtat
     9901 cgtccaaatc gggcgcgtcg ccgctcatat tgttgtacac ctgaccaaac gccgaaccgc
     9961 ccatacgcgc tttgccgaag cccaaatcga caaacaacaa tacgctgtct tcgacgtttt
    10021 tcaactcagg cgtaacagtc ttgcgtacgt ctttcacagg cgcgaacgct gagataatca
    10081 ggctcaacgg tgaaaccacg gattttttct cctcgccgtc ctgccaaacg gttttcatcg
    10141 acaggctgtc tttgcccacg gggatgctca aatccaatgc ctgacaggct ttggaaacgg
    10201 cttcgacagt gcggtagagt ttttcgtctt cgccttcgtt gccgcacgcc gccatccagt
    10261 tggcggagag tttgatgttg ccgatgtctc cgatgttgac cgccgcgatg ttggtgatgg
    10321 cttcgccgac gcacattctg cccgaagcag gcgcatcaaa cagggcgacg gtcggttttt
    10381 cgcccataga catcgcttca ccgcgatagg tgttgaagcc catcatggta acggcgcagt
    10441 cggctactgg agtttgatat ttgccgacca tttggtcgcg gtgcgtcaaa ccgccgacgc
    10501 tgcggtcgcc gatggtaatc aggaagtttt tggcggctac ggcaggcagg cgcaaaacgc
    10561 ggtaggcggc ttcggtaatg tcgatatcgc ccgcgtgaaa cggttttttg gacggtgcaa
    10621 ccgttttgtc ggtgcgcgtg gttttgggca gtttgccgag caagacgttc aacggcaaat
    10681 cgacgggatt gttggcgaac aaatcgtcgc gtactttcaa atgaccgtcg tcagtcgccg
    10741 tgccgactac ggcaaacggg cagcgttcgc gttcgcagat ggcgcggaaa gcatccaaat
    10801 ctttttccaa aatcgacaac acataacgct cttgcgattc gttgcaccag atttgcagcg
    10861 ggttgaggcc gtgttcttca agcggcactt cgcgcagctt gaatactgcg ccgcgtctgg
    10921 catcgttgac cagttcgggg aaggcgttgg acaggccgcc cgcgcctacg tcgtggatgg
    10981 agataatcgg gtttttgccg ccgagctgcc agcagcggtc gatgacttcc tgcgcgcggc
    11041 gttcgatttc ggggttgccg cgttgcacgg agttgaagtc caaagacgcg tcgtttgtgc
    11101 cggtatccat cgaagaagcc gcgccgccgc ccaagccgat aagcataccc gggccgccca
    11161 gttggatcag caatgcgcct tcggggattt cgtctttatg cgtctgctgc gcctgaatgc
    11221 tgcccaagcc gccggcaatc ataatcggtt tgtgatagcc gcgaacctga ccgtcaaatt
    11281 tttcttcaaa agtgcggaag tagcccaaga ggttggggcg gccgaattcg ttgttgaacg
    11341 ccgcgccgcc gatcgggcct tcaatcatga tgtccagcgg cgaggaaata tgttccggct
    11401 tgccgtagtc ttgttcccac ggctgtttga ggtcgggaat attgaggttg gacacggtaa
    11461 agccggtcag gcccgctttc ggacgcgaac ctttgcccgt cgcgccttcg tcgcggattt
    11521 cgccgcccgc gcccgtcgcc gcacccgcaa acggcgcgat ggcggtcggg tggttgtgcg
    11581 tttccacttt catgatgata tgcgtgtctt cctcgtggaa acggtagcct tggttttccg
    11641 ccgcattcgg atagaaacgc tcgattttcg cgccttcgat tacggacgaa ttgtctttat
    11701 aggcaacgac cgtgccttcg ggatgcgcgt tgtgtgtgtc gcgtatcata ccgaagaggg
    11761 atttgggctg cttttcgccg ttgaggatga aatcggcgtt gaagattttg tggcggcagt
    11821 gttcgctgtt tgcctgcgcg aacatcatca attcaacatc ggacggattg cgctgcaaag
    11881 cctgatagtt ttcgaccaga taatcgattt cgtcggcgga aagtgccaag cccatttcgg
    11941 tattggcttt gaccaaagct tctttaccgc cgcccaaaac atcgacgccg gagaaagttt
    12001 cggattcgag atggtggaat aatttggagg ccgtctgaaa atcgggcagc acgctttcgg
    12061 tcatgcggtc gtgcagcaaa gccgcccatt gctgtttctg ttcatcgttc agacgacctt
    12121 ccagccacac cgccataccg cgctcgatgc gttcgatgcc tgccaaaccg cagttttccg
    12181 cgatattggt cgccttggaa gcccacggcg aaatcgtacc caaacggggc gtgaccaaaa
    12241 acaaatgcaa gccctcgcgc gcttttggcg tttgttcaac gctttgcgcc gccaacaagg
    12301 cttgcagttt ttcgacagtc gcggcatcaa gtgctttctc gctgccgacg aaataccaaa
    12361 attcgctgct taatttgact tcgggcagac cgagtgcggc ggctttttgc aagagttttt
    12421 caacacggaa atcggaaagg gcggtaacgc cgcgcaaggg caaaacgaca gacatggatt
    12481 cggctctcaa atgcggttgg aaaatctgtc attatacgcg caacggggct gtattgcttg
    12541 ggtattttgg cttaaaatgt tgacaatctg acgggaggtt cccgtttttc ggatgtgttt
    12601 gtattgtttg ttcaaaggaa attttatgaa aaaaatcgag gcgattgtca aaccgttcaa
    12661 actcgacgac gtgcgcgagg cgttgacgga aatcggcatt acgggcatga ccgtcagcga
    12721 ggtcaaaggg ttcggcaggc agaaggggca tacggaaatc tatcgcggcg cggaatacgc
    12781 cgtcgatttc ctgcccaaaa tcaaaatcga gctggtgttg gcggatgatg ctgtggaacg
    12841 cgcgattgac gtgattgtcg aggtggcgcg ttcgggaaaa atcggcgacg gcaagatttt
    12901 tgtgctgccg gttgaggagg caatccgtat ccgcacgggc gaacgttcgg acgcggcagt
    12961 ctgacggccg gatagaaaat aaaaaccgca gccttgcagg gctgcggttt ctttatcggg
    13021 agatgctgct taccactcgt aattgacgcc gacatggtag gctgcggaag aaccggacga
    13081 agtgccgact gccacgcctg ctttggcggc aaagttttcg gtaaagcgga agccggtacc
    13141 gatggcgact gccgattcgg atttgtagcc gccgactgca gccgttacat tgaaccgacc
    13201 cacgttgtaa ggttggaaca ggccggagag cgcggcttgt tctgcaaggc cttggcgggt
    13261 ttctttgcgc agattagcta cgtttttgtc caagctgtcg atgcgtgctg agtttttagc
    13321 aatatcagct ttgttcgtag cgatatcagc tttgatgtcg gtaacttttg cagcgacagc
    13381 ttcggccttg tcggctgcag tattagctgt gccagcggca gcttcggctt tgcctgctgc
    13441 agtttctgca gcttttactt tggcatcgac gttttgtttg gtttcttcgg ccgtctgttt
    13501 ggcttcattg gcggttttga cggcttcgtc tgccttagtg ttggtttcat ccaatgaatc
    13561 ggcgatatcg ttgaatgctt cggcatgctt gtcgacggta tcagccacgg cttctaattt
    13621 ttcatcaatt tttacgatat ttgtcttagt ctcttcagca aatgtcgtta tattttctcc
    13681 caatttattc aaggcgttgg tggtttcatc cagagcggca tcagtatctg ctaaagcggc
    13741 atcagtgtct gctaacttgg ttgttaactt ttctatttca gattctgcag cttttacttt
    13801 ggcatcgacg ttttgtttgt tttcattgac ggttttggtc aggttagtca cgactttttt
    13861 cagacccaga cctttaaagt cgtcggcttc aacatcggct gcagttgcgt ctttttgggt
    13921 aattgtgccg tcttcaccaa tgtcgtagat ggtctctcca gctttgaaac cgttgatttc
    13981 ttggccattg ttgtaggcag caacaatggc cacagtggca gcttttttaa catcgtcgtc
    14041 gcttgtggct gccagtgcgc cgctacagaa agtggcaagg atggctgtgg tcagtacttt
    14101 ggatggaaag tgtttcatgc tcattacctt tgtgagtgga taaaaaatgg ggcggaaatc
    14161 ttttgtaagt attttatccc caccaagttt gtttacatat taaagtatgt atgaaaggag
    14221 gcatatatac aatacattgt cgcaatttat ttatttattt atttaattct tgcagttagg
    14281 tagttcggca ttaatatctg ttaatatgtg catattatgc tgatgtcaat tctgccggtg
    14341 tcgtgtctta attttgtcgg gatatgggtt gccgtctgaa tgttcagacg gcctttttgt
    14401 cggatttaga tatggtggat aaccaatttt ttgccttcgt aatcgaggac gttgacgtcc
    14461 atatcgaata aggttttgat gttttcggcg gtgaaaatat cgttgggttt gccctgcatg
    14521 gcgacttggc cgtttttcat ggcgacgacg taatcggcgt aggcggcggc ctggttgatg
    14581 tcgtgcaata cggcgacggt ggtgcgtttg tgttcgcgcg tgagcttttg cagaatctgc
    14641 atgagcgagc gggcgtgata catatcgagg ttgttcagcg gttcgtccaa aaggacgtag
    14701 tcggtgcttt ggcagaacac catcgcaatc atggcgcgtt ggcgttgtcc gccggagagt
    14761 tcggtcagat agcggtcgga gaggtcctgc aggtggaatt cttcgattgc accgttaacg
    14821 atacggcggc attcggcagt cggtctgcct tgatggtagg ggtaacgtcc gaacatcagc
    14881 aggtcgcgca cggtgatgcg gctcatgatg ctgttttctt gggtgaggat ggagagcgtg
    14941 cgggcgagtt cggcggtggg agtgccggca aggtttttgc ctcggtaggc gatgctgccg
    15001 ctttcaagcg ggcgcagccg cgccataaag gaaaacaggg tggatttgcc tgcgccgttc
    15061 gggccgacga gggcggtaat gccgccttcg gggatgtcga ggctgacgtt gtcgaggatg
    15121 gggcgtgtgc cgatgcggta gctgacgttg cggatggtaa tcatggcgga tgcttgtcgg
    15181 gcgtaggttt gttgtatgtt tggcgggggt tcagacggca tttgaaattc ggaatgccgt
    15241 ctgaacgctc acacttcgcg gacgatgacg aactgcgggt cgtccgccga ttcggtcaaa
    15301 aatgctttaa cgcgtgcctg ccagagttgt ccgaagcgtt cgagttcttc tgcgcttgcg
    15361 ctgccgccga cggctttggg catgatgtcg cgcatttcgg gtgcgaaggg ttgcagggcg
    15421 gcgttgaggc cgacggcgac ggttttgccg ttgtctttgc ggcgcagggt cagtgtgccg
    15481 ttgatttcgc ctacaccaaa ggataagagg tggcggcggg caaagcgtcc ctgcattccg
    15541 atgccgccaa agccggtttc gggggctgcg ccggtgagga gttggacgac ggacgcggtt
    15601 acgccgaccg tgccttcgtc gcgcgctccc tgcatggcgg cttcgatgcc gccgcgttcg
    15661 ggcagctctt cgccgtaaag ggctttcaga cctttgataa ccatcaggta cgcgcccgcg
    15721 acggtcgggc aggaatgtcc gcacaggcgc acggcatcgg cgtagcggta agtgaggatg
    15781 ccgttttcgg ccgcgccgag gaatgcggca agcgggtctc taacggtgat ggtcggggct
    15841 tggttgaaga atgatgggaa atgttcttgt gtcatatggt ttgtcctttc gaggcgggcg
    15901 gccgttcaga cggcatccgt cattttttgt gttttaaaac gagatagagg aaaacgagtc
    15961 cgcccgcaaa ttcgaccacc acgcttaata ccgccttcat gcccaagaag tgttcgaata
    16021 cggtttgtcc gccgaccagg aggataccgc cgacacaaac cgtcatcggc aggcggacgg
    16081 aatggcgcac ggacggggaa aagtggttgg caagcgaggc ggcgagaagc ccgaaaaagc
    16141 tcaccgggcc gaccacggcg gtcgccgtcg ccaccagcgc ggcaatccaa agcagtatcc
    16201 ataaggtgtt gcgcgtgtag ctgatgccca aattgacggc ttggtcgcgc cccaaaaggt
    16261 gtacgtccaa gcggtagcgt tcgcgccaaa ccgccgccgc gctgatgagc agaatcagcg
    16321 cgcctatccc caacagttcg ttgtggacgg tattgaaact ggcgaacatg tttgcctgcg
    16381 ccgcggtaaa ctcttcggga tcgatcatgc gggagagcag cgaggaaagg ctgcggaata
    16441 aaatcccgaa aatcacgccg attaaaatca tgcgcgacaa atcgcgcccg ccttgtttga
    16501 cgagcgtgta gaacagcagc agcgagccgc ccatcatgac ggcaagttcg aagccgaact
    16561 tgccgaccgc aggcagggac gtatagccca cgccgccgag cgtaaacacc agcaaggtct
    16621 gcaaaaacac atacagtgaa tcaaaaccca aaatcgaagg ggtcagaatc ggattgttgg
    16681 tcagcgtttg gaagagttgc gtggacacgc cgaccgcata ggcgaccatc agcagcgcgg
    16741 caagtttggt cagccgcagg tgcaaaacaa aatcccaatc gcctttgacg ttgagcgtca
    16801 taaacaggac gcaggaaacc agcaacagcg caaaggcgac ccacaacgga cggctgcttc
    16861 ctgccataaa accgatattt ttttcagacg gcatgagcag gtttcctcaa caaaagccac
    16921 aaaaacaaag ccgtccccat cacgccaaaa accgtagaga ccggaatttc aaacggaaac
    16981 acaatcacgc gtccgacaat atcgcacagc aacaccaaag atgcgcccag caaggccacc
    17041 gcaggcaggc tttgtcgcag cttgtcgccc atcaggcggc tgatgatgtt cggcacgacc
    17101 agcccgataa acgggatatt gccgaccgta acgataacca gcgacgtaat caaagccaca
    17161 ataatcagcc ccgaccacaa caccgccgtc cggttcaaac ccaaattcac gctgaccgtc
    17221 tcgcccagcc ccaaaatcgt cagccggtcg gcaatcagat aggcaaacac cgccaaaccg
    17281 cccgtaatcc aaagcagctc gtaccgcccc agcagcacgc tcgaaaaatc gccctgctgc
    17341 cacacgccga gcatttgcag catttcgttt tcatacgcga taaacgtcgc cactgcctca
    17401 accacgccgc cgaaaataat ccccaccagc ggcaccatca gttgcgccgt cggcggcaga
    17461 cggcggatca gcagcataaa caccagcatc ccgatcagcg cggcaacggc ggcaaccgac
    17521 attttgaccg gcagcggcgc ggcaggcagc agcagggaca tcagaagcaa acccaaagcc
    17581 gcgctttgac ccgcgcccgc catagaaggc tcgacaaaac ggttacgcat caaaatctgc
    17641 ataatcatcc ccgccaccgc catcgacgcg cccgtcagca caatcgcaaa cgtgcgcggc
    17701 aggcggctga tgaacataac ctgctggctg tccgacagcg aaaacacatc cgaccagcgg
    17761 aaatcggcaa cgccgaccga caggctgacg gcaaacaaca ccgccagcag caggaggttg
    17821 gtcaggttga gggaaaaagg tttggcagtc ataaacagaa gggaaaagcg ttaaggcgta
    17881 gaagattcaa acaaggcagt ccgaaccgtc ggagcggaaa gccttgtttg aagcctccgt
    17941 atcgggcaga ggatagggca agttccaata tagtggatta acaaaaacca gtacggcgtt
    18001 gcctcg
//
LOCUS       DAWISC010000023        17307 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWISC010000023 DAWISC010000000
VERSION     DAWISC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.023"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..939
                     /gene="tuf"
                     /locus_tag="WIW87_000543"
     CDS             <1..939
                     /gene="tuf"
                     /locus_tag="WIW87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5173742.1"
                     /translation="PGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQV
                     GVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAA
                     YEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDE
                     IEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTIT
                     PHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVT
                     ITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            946..1021
                     /locus_tag="WIW87_000544"
     tRNA            946..1021
                     /locus_tag="WIW87_000544"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Trp,seq:cca)
     gene            1136..1414
                     /gene="secE"
                     /locus_tag="WIW87_000545"
     CDS             1136..1414
                     /gene="secE"
                     /locus_tag="WIW87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ5173743.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            1416..1952
                     /gene="nusG"
                     /locus_tag="WIW87_000546"
     CDS             1416..1952
                     /gene="nusG"
                     /locus_tag="WIW87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ5173744.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            2053..2487
                     /gene="rplK"
                     /locus_tag="WIW87_000547"
     CDS             2053..2487
                     /gene="rplK"
                     /locus_tag="WIW87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ5173745.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            2487..3182
                     /gene="rplA"
                     /locus_tag="WIW87_000548"
     CDS             2487..3182
                     /gene="rplA"
                     /locus_tag="WIW87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ5173746.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            3412..3912
                     /gene="rplJ"
                     /locus_tag="WIW87_000549"
     CDS             3412..3912
                     /gene="rplJ"
                     /locus_tag="WIW87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ5173747.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3970..4341
                     /gene="rplL"
                     /locus_tag="WIW87_000550"
     CDS             3970..4341
                     /gene="rplL"
                     /locus_tag="WIW87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ5173748.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            4531..8709
                     /gene="rpoB"
                     /locus_tag="WIW87_000551"
     CDS             4531..8709
                     /gene="rpoB"
                     /locus_tag="WIW87_000551"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ5173749.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDFGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8866..13041
                     /gene="rpoC"
                     /locus_tag="WIW87_000552"
     CDS             8866..13041
                     /gene="rpoC"
                     /locus_tag="WIW87_000552"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ5173750.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            13333..13590
                     /locus_tag="WIW87_000553"
     CDS             13333..13590
                     /locus_tag="WIW87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173751.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMRMPINIG"
     gene            13587..13736
                     /locus_tag="WIW87_000554"
     CDS             13587..13736
                     /locus_tag="WIW87_000554"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5173752.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13915..14286
                     /gene="rpsL"
                     /locus_tag="WIW87_000555"
     CDS             13915..14286
                     /gene="rpsL"
                     /locus_tag="WIW87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ5173753.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            14404..14874
                     /gene="rpsG"
                     /locus_tag="WIW87_000556"
     CDS             14404..14874
                     /gene="rpsG"
                     /locus_tag="WIW87_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ5173754.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14893..16998
                     /gene="fusA"
                     /locus_tag="WIW87_000557"
     CDS             14893..16998
                     /gene="fusA"
                     /locus_tag="WIW87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ5173755.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            17084..>17307
                     /locus_tag="WIW87_000558"
     CDS             17084..>17307
                     /locus_tag="WIW87_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5173756.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         4611 a         3892 c         4468 g         4336 t
ORIGIN      
        1 ccggggcacg ccgactacgt taaaaacatg attaccggcg ccgcacaaat ggacggtgca
       61 atcctggtat gttccgcagc cgacggccct atgccgcaaa cccgcgaaca catcctgctg
      121 gcccgccaag taggcgtacc ttacatcatc gtgttcatga acaaatgcga catggtcgac
      181 gatgccgagc tgttggaact ggttgaaatg gaaatccgcg acctgctgtc cagctacgac
      241 ttccccggcg atgactgccc gattgtacaa ggttccgcac tgaaagcctt ggaaggcgat
      301 gccgcttacg aagaaaaaat cttcgaactg gctgccgcat tggacagcta catcccgact
      361 cccgagcgag ccgtggacaa accgttcctg ctgcctatcg aagacgtgtt ctccatttcc
      421 ggccgcggta cagtagtaac cggccgtgta gagcgcggta tcatccacgt tggtgacgag
      481 attgaaatcg tcggtctgaa agaaacccaa aaaaccactt gtaccggtgt tgaaatgttc
      541 cgcaaactgc tggacgaagg tcaggcgggc gacaacgtag gcgtattgct gcgcggtacc
      601 aaacgtgaag acgtggaacg cggtcaggta ttggctaaac cgggtactat cactcctcac
      661 accaaattca aagcagaagt atacgtactg agcaaagaag agggtggtcg tcacactccg
      721 ttcttcgcca actaccgtcc gcaattctac ttccgtacca ccgacgtaac cggcgcggtt
      781 actttggaag aaggtgtgga aatggtaatg ccgggtgaaa acgtaaccat caccgtagaa
      841 ctgattgcgc ctatcgctat ggaagaaggc ctgcgctttg cgattcgcga aggcggccgt
      901 accgtgggtg ccggcgtggt ttcttctgtt atcgcttaag tttagaggcc aatagctcaa
      961 ttggtagagt atcggtctcc aaaaccgagg gttgggggtt cgagaccctc ttggcctgcc
     1021 aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt gtatttgtta tttagtaaac
     1081 tctcttgcca tttacatgga ttgagaatag acagatgcta tgatggataa ataatatgac
     1141 agaacatacg cctgaaaaaa agaacgttaa agtggatcaa ctggttgttc aagataaaga
     1201 atctgcatct aattccggta aggaagggtt ttttgcatat ttctcaaatt cttggtccga
     1261 attcaaaaag gtggtttggc ctaagcgtga agatgctgtc agaatgactg tatttgttat
     1321 agtgtttgtt gctgtgcttt ctatatttat ctatgcggca gatacagcaa tttcgtggtt
     1381 attttttgat gtattgctga gaagggaagg ttgagatgtc gaaaaaatgg tatgttgtac
     1441 aggcgtattc ggggtttgag aagaatgtcc aacgaatatt ggaagagcgc attgcccgtg
     1501 aggagatggg agattatttc ggacaaattc tggtgcctgt agagaaagtt gttgatatcc
     1561 gcaatggtcg taagactatt agtgaaagaa agtcatatcc tggttatgtg ctagttgaga
     1621 tggaaatgac agatgactct tggcatcttg taaaaagcac cccccgtgtt tccggtttta
     1681 ttggagggag ggctaataga cctacgccga ttagtcagag agaggctgaa attattttac
     1741 agcaggttca gaccggcata gagaagccga aaccaaaagt tgaatttgag gtcggtcaac
     1801 aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg ggtggttgag gaggtcaatt
     1861 atgaacggaa taagttacgc gtgtctgttc agatatttgg tagagaaaca cccgttgagc
     1921 tggagttcag ccaggttgaa aagattaact gatttttata cttgaaaaaa aagcaataag
     1981 aggatagaat caaaaattaa cttggggagc ggaaatggtt ccgcgtctta cccgttttta
     2041 ggagttcgtt aagtggcaaa gaaaattatc ggctatatta aactgcaaat tcctgcaggt
     2101 aaagccaatc catctcctcc ggttggtcct gctttgggtc agcgcggttt gaatattatg
     2161 gaattttgta aggcatttaa tgctgcaacc caaggtatgg agcctggctt accgattccg
     2221 gttgtgatta ctgcatttgc agataaatca ttcacatttg tgatgaaaac cccgccagct
     2281 tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta gttctaatcc tctgaccaac
     2341 aaagtgggta aattgacccg tgcccagttg gaagaaattg ctaaaactaa agatcctgat
     2401 ttgactgctg ctgacttgga tgcggctgtc cgtactatag caggttctgc tcgctcaatg
     2461 ggcttggatg tggagggtgt tgtataatgg ctaaagtatc taaacgcttg aaagctcttc
     2521 gctcttctgt ggaagccaat aaattatatg caattgatga agcaattgct ttggtaaaaa
     2581 aagcagcgac tgctaaattt gacgagtctg ttgacgtatc tttcaacttg ggcgttgatc
     2641 cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct gcctaaaggc accggtaaga
     2701 taacccgtgt ggctgtattt actcaaggtg caaatgcaga agctgctaaa gaagctggtg
     2761 cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa agcaggcaat ctgaactttg
     2821 atgtcgttat tgcttctccc gatgcaatgc gtattgttgg tcagttgggt actattttgg
     2881 gtcctcgagg cttgatgcca aaccctaaag taggtacggt tactcctaac gttgctgaag
     2941 cagttaagaa tgcaaaagca ggtcaagtac aataccgtac agataaagca ggtatcgttc
     3001 atgcaacgat tggtcgtgct tctttcgctg aagctgattt gaaagagaac tttgatgcgt
     3061 tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa aggtcagtat ctgaaaaaag
     3121 ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga tacatcaagc gtaaataact
     3181 aatcttaagg aattttcaag cagtttggtt ttctgggctg cttgaatttg ggctacttaa
     3241 aattaagtag atgtccaaga ccgtagggat cgtaagattt aatcgtaact gccctacgca
     3301 gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga tgtcttttta ggttaccgcg
     3361 ctggtgggat atcgttttgg tatcctgttt ataaacagtg ggaggtagac cttgagtctc
     3421 aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg cggcaattgc taatgctcaa
     3481 accctcgtag tcgctgaata tcgcggtatc agtgtttcca gtatgactga gcttcgtgcg
     3541 aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa atactttggc tcgtcgtgca
     3601 gtgcaaggta cttcatttgc agaattggcc gatcaaatgg ttggtccgtt ggtttacgct
     3661 gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc aattcgcgaa aaaagatgac
     3721 aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa tgaatgctgc tcaggttgct
     3781 gagttggctt ctattccgag ccgcgaagag ctgttgtcca aactgttgtt cgttatgcaa
     3841 gctcctgtat cgggctttgc gcgcggtttg gctgctttgg cagagaaaaa agccggcgaa
     3901 gaagccgctt aatcgatttt gtttctgtta atcaattatt ttttaataca atatttggag
     3961 taaaatagca tggctattac taaagaagac attttggaag cagttggttc tttgaccgta
     4021 atggaattga acgacttggt taaagctttt gaagaaaaat tcggtgtttc tgctgctgct
     4081 gttgcagttg caggtcctgc tggtgccggt gctgccgatg ctgaagaaaa aaccgaattt
     4141 gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg tgattaaagt tgtccgtgca
     4201 attaccggtt tgggtctgaa agaagctaaa gacatcgttg acggcgcacc taaaaccatt
     4261 aaagagggtg tttctaaagc tgaagccgaa gacatccaaa aacaactgga agaagcaggc
     4321 gctaaagtcg aaatcaaata atttgatgct tcttatgaag gctggcagtt ttctgccagc
     4381 cttattttgc ttcttaaaat aaacatcaag tattgtttac atttatttgc atagttttta
     4441 tcaagtcatt gcaaataaat gtaaatatca gattgatgcg taccgttgtt tcagacggcc
     4501 tattattgaa aattactttt cggagtgtgt atgaactatt cgtttaccga gaaaaaacgt
     4561 atccgtaaga gttttgcaaa gcgggaaaat gttttggaag ttcctttctt gctagcaacc
     4621 caaattgatt cttatgcgaa gtttttgcag ctggaaaatg cttttgacaa acgtaccgat
     4681 gacggtctgc aggcggcatt taattctatt ttcccgattg tgagccataa cggttatgcg
     4741 cgattggagt ttgtgcatta cacattgggc gagcctttgt tcgatattcc cgaatgtcag
     4801 ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta tccgtttggt gattttggat
     4861 aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa acgaagtgta tatgggcgaa
     4921 attccgttga tgaccccgag cggttctttt gtgattaacg gcacagagcg tgtgattgtc
     4981 tcccagttgc accgttcgcc cggcgtattc ttcgagcatg acaaaggtaa gacgcactct
     5041 tccggcaaat tgttattctc cgcccgcatc attccctacc gtggttcatg gttggatttt
     5101 gaatttgatc cgaaagattt gctgtatttc cgtatcgacc gccgccgtaa aatgccggta
     5161 acgattttgt tgaaggcttt aggctacaac aatgagcaaa tcttggatat tttctacgac
     5221 aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg atttggttgc agaccgtctg
     5281 aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag gcaatgtatt ggttgccaaa
     5341 ggtaagcgca ttactgcgaa aaatatccgt gatattacca atgcaggcct gacccgtttg
     5401 gatgtagaac cggaaagcct gctgggcaaa gcattggctg ccgatctgat tgattcggaa
     5461 accggcgagg tattggcttc tgccaatgat gaaattacag aagagttgtt ggccaaattt
     5521 gatatcaacg gcgtaaaaga aattacgacc ctttatatca atgagctgga tcagggtgct
     5581 tatatctcca ataccttgcg tacggatgag actgccggcc ggcaggcggc tcgtgttgcg
     5641 atttaccgta tgatgcgtcc gggcgaaccg cccaccgaag aggcggtcga gcaattgttt
     5701 aaccgcttgt tcttcagtga agacagctac gatctgtccc gcgtaggccg tatgaaattt
     5761 aatacgcgca catacgaaca aaaactgtcc gaagcccaac aaaactcttg gtacggccgc
     5821 ctgctgaacg aaacgtttgc cggtgctgcc gacaaaggcg gttatgtcct gagcgtcgaa
     5881 gatattgtcg cctcgattgc gactttggtc gagttgcgta acggccatgg cgaagtggac
     5941 gatatcgatc acttgggcaa ccgccgagta cgttcggtag gcgagctgac tgaaaaccaa
     6001 ttccgtagcg gtttggcccg tgtggaacgt gccgtaaaag aacgtttgaa tcaggcggaa
     6061 tcagaaaact tgatgccgca cgatttgatt aatgcaaaac ctgtttctgc cgctattaaa
     6121 gaattcttcg gctccagcca attgagtcag tttatggatc agaccaaccc cttgtctgaa
     6181 gtaacccata aacgccgtgt atctgcattg ggtccgggcg gtttgacccg cgaacgtgca
     6241 ggatttgagg tgcgggacgt gcatccgacc cactacggtc gcgtatgtcc gattgaaacg
     6301 cctgaaggtc cgaacatcgg tttgatcaac tcattgtccg tttatgcgcg caccaatgat
     6361 tacggtttct tggaaacgcc ttaccgccgc gttatcgacg gcaaagtaac cgaggaaatc
     6421 gattacttgt ctgccatcga agaaggccgc tatgtgattg cacaggcgaa tgccgatttg
     6481 gattcagatg gcaatctgat tggcgatttg gttacctgtc gtgaaaaagg cgaaaccatt
     6541 atggcaacgc ccgaccgcgt ccaatatatg gacgtggcaa ctggtcaagt ggtatccgtt
     6601 gcagcatccc tgattccatt cttggaacat gatgacgcga accgcgcatt gatgggtgcc
     6661 aacatgcaac gtcaggcagt gccttgcttg cgtcctgaaa aaccgatggt cggtaccggt
     6721 atcgagcgtt ccgttgccgt tgactctgct actgcaatcg ttgcccgccg aggcggcgtg
     6781 gtcgagtatg tcgatgccaa ccgcgttgtg atccgtgtcc atgacgacga agcgactgcc
     6841 ggtgaagtgg gtgtcgatat ttacaacttg gttaaattca cccgttccaa ccagtctacc
     6901 aatatcaatc agcgtcctgc cgtcaaagcc ggcgatgttt tgcaacgcgg cgatttggtg
     6961 gccgacggcg cgtccaccga ttttggcgaa ttggctttgg gtcaaaatat gaccatcgcc
     7021 ttcatgccgt ggaacggtta caactacgaa gactcgattc tgatttccga aaaagtggct
     7081 gcggacgacc gctatacttc gattcacatt gaggaattga atgtcgttgc ccgcgatact
     7141 aagctgggtg cggaagacat tacccgcgat attccgaact tgtccgagcg tatgcaaaac
     7201 cgtttggacg aatccggtat cgtttacatc ggtgcggaag tagaagccgg cgatgtgttg
     7261 gtaggcaagg taacgcctaa aggcgaaacc caactgacgc cggaagaaaa actgctgcgc
     7321 gccatcttcg gtgaaaaagc atctgacgta aaagatactt cattgcgtat gcctaccggc
     7381 atgagcggta ccgttatcga cgttcaagtc ttcactcgtg aaggtattca acgcgacaaa
     7441 cgtgctcaat ccattatcga ttccgaattg aaacgctacc gtttggattt gaacgaccaa
     7501 ttgcgtattt tcgacaacga cgcattcgac cgtatcgagc gtatgattgt cggtcagaaa
     7561 gccaacggtg gtccgatgaa gctggccaaa ggcagcgaaa tcacgaccga atatctggcg
     7621 ggtctgccga gcaggcacga ttggttcgat atccgtctga ccgatgaaga tttggccaag
     7681 cagttggaac tgattaaagt gagcctgcaa caaaaacgcg aagaagcgga cgagttatac
     7741 gaaatcaaga agaaaaaact gacccaaggc gacgaattgc aacccggcgt acaaaaaatg
     7801 gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg gcgacaaaat ggcgggccgc
     7861 cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg aagacatgcc ttacatggcg
     7921 gacggccgtc cggtagacat cgtactgaac ccattgggcg taccttcccg tatgaacatc
     7981 ggtcagattt tggaagttca cttgggttgg gcagcaaaag gtatcggcga gcgtatcgac
     8041 cgtatgctga aagagcaacg caaagcaggc gagttgcgcg agttcttgaa cagactctac
     8101 aacggcagcg gtaagaaaga agatttggat gccctgactg atgaagaaat catcgaactg
     8161 gcctccaacc tgcgcaaagg tgcatctttc gcctctcctg tattcgacgg tgcgaaagag
     8221 tctgaaatcc gcgaaatgct gaacttggct tatccgagcg acgatcctga ggttgaaaaa
     8281 ctgggcttca acgacagtaa aacccaaatc acgctgtatg acggccgttc aggcgaagca
     8341 tttgaccgca aggttacagt aggtgtgatg cactatctga aactgcacca cttggttgac
     8401 gaaaaaatgc acgcgcgttc taccggtccg tacagtctgg ttacccagca gcctttgggc
     8461 ggtaaagccc agttcggcgg ccaacgtttc ggcgagatgg aggtttgggc attggaagca
     8521 tacggcgcgg catacacgct gcaagagatg ctgactgtga agtctgacga cgtgaacggc
     8581 cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca aaatcgatgc cggtatgccc
     8641 gagtccttca acgtattggt caaagagatt cgctcactgg gcttggatat cgatttggaa
     8701 cgttactaaa caaaagtttt cagacggcct ttcagggtcg tctgaaaaag tggtttcaga
     8761 ataagaatga agcaatcggc atttaggccg tctgaaatca aaagtaccgt ttcccaatat
     8821 cgaaaatccg ccatgcggta aaaatacttc cttcaaggag caaaaatgaa tttgttgaac
     8881 ttatttaatc cgttgcaaac tgccggcatg gaagaagagt ttgatgccat taaaatcggt
     8941 attgcctctc ccgaaaccat ccgctcatgg tcttatggcg aagtcaaaaa acctgaaacc
     9001 atcaactacc gtacgttcaa acctgagcgt gacggtttgt tctgtgccaa aatctttggc
     9061 ccggtcaaag actacgaatg cttgtgcgga aaatacaaac gcttgaaatt taaaggcgta
     9121 acgtgtgaaa aatgcggcgt ggaagtaacc ctgtccaaag tgcgccgcga acgcatgggt
     9181 catatcgaat tggctgcgcc cgtcgcacat atttggttct taaaatccct gccttcccgc
     9241 ttgggtatgg tgttagacat gactttgcgc gacatcgagc gcgtattgta ctttgaagca
     9301 tttgtggtaa ccgatcccgg tatgactccg ctgcaacgcc gccaattgct gactgaagac
     9361 gattactaca acaagctgga cgaatacggc gacgatttcg atgccaaaat gggtgcggaa
     9421 ggtatccgcg aattgctgcg taccctgaat gtagcgggcg aaatcgaaat cctgcgccaa
     9481 gagttggaat cgaccggttc cgacaccaaa atcaaaaaaa tcgccaaacg cttgaaagta
     9541 ttggaagcct tccatcgttc cggtatgaaa ctggaatgga tgattatgga tgtgctgccg
     9601 gtattgccgc ctgatttgcg tccgttggtt ccattggatg gtggtcgttt tgccacttcc
     9661 gatttgaacg atttgtaccg ccgcgttatt aaccgtaaca accgtctgaa acgtctgttg
     9721 gaactgcatg cgcctgacat catcgtccgc aacgaaaaac gtatgttgca agaagcagtt
     9781 gactcgctgt tggataacgg ccgtcgcggt aaagccatga ccggcgccaa caaacgcccg
     9841 ctgaaatcat tggcagacat gattaaaggt aaaggcggtc gcttccgtca aaacctgttg
     9901 ggcaaacgtg tggactactc cggccgttcc gtgattaccg taggcccgta cctgcgtctg
     9961 caccaatgcg gtttgccgaa aaaaatggct ttggaactgt tcaaaccgtt cattttccac
    10021 aaattggaaa aacaaggttt ggcctctacc gttaaagcag cgaaaaaatt ggtagagcaa
    10081 gaagtaccgg aagtatggga catcttggaa gaagtcatcc gcgaacatcc gattatgctg
    10141 aaccgtgcgc cgaccctgca ccgtttgggt attcaagcgt tcgaacctat cttgattgaa
    10201 ggtaaagcga ttcagttgca cccattggtg tgtgctgcgt tcaacgccga ctttgacggc
    10261 gaccaaatgg cggtacacgt tccattgagc ttggaagcac aaatggaagc acgcacgctg
    10321 atgctggctt caaacaacgt attgtctccg gccaacggcg aaccgattat cgtaccttcc
    10381 caagacatcg tattgggcct gtactatatg actcgcgatc gtatcaatgc caaaggcgaa
    10441 ggcagcctgt ttgccgatgt gaaagaagtg catcgcgcat accataccaa acaggtcgag
    10501 ctgggtacga aaatcaccgt acgtctgcgc gaatgggtga aaaacgaagc aggtgagttt
    10561 gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg cattgttgag cgaaatcctg
    10621 ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga agaaaaaaga aatttctaaa
    10681 ctgattaacg catcgttccg cctgtgcggc ttgcgcgata cggttatctt tgctgaccac
    10741 ctgatgtaca ccggtttcgg atttgcggca aaaggcggta tttccattgc cgttgacgat
    10801 atggaaattc caaaagaaaa agcggccttg ctggctgaag ccaatgccga ggttaaagaa
    10861 atcgaagacc aataccgtca aggtttggtt accaacggcg aacgctacaa caaggtggtc
    10921 gatatttggg gtcgtgccgg cgataagatt gctaaagcga tgatggacaa cttgtccaaa
    10981 caaaaagtta tcgaccgtgc cggcaacgaa gtcgatcaag agtcattcaa ctccatttat
    11041 atgatggcgg actccggtgc ccgtggttct gcagctcaga ttaaacagtt gtccggtatg
    11101 cgtggcttga tggcaaaacc tgacggctcg attattgaaa cgccgattac ctcaaacttc
    11161 cgtgaaggtc tgaccgtatt gcaatacttt attgcgaccc acggtgcgcg taagggtttg
    11221 gcggataccg cattgaaaac cgcgaactcc ggttacctga ctcgtcgtct ggtagacgta
    11281 actcaagatt tggtcgttgt tgaagacgat tgcggtactt cagacggctt tgtcatgaag
    11341 gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg atcgtatttt gggtcgtgtt
    11401 accgcgtctg acgttgtcga tccgtcaagt ggcgaaacct tggttgaagc cggtacgttg
    11461 ctgactgaaa aactggtgga tatgatcgac caatccggtg tcgatgaagt caaagtccgt
    11521 acgccgatta cttgtaaaac ccgtcacggc ctgtgtgcac actgttacgg tcgtgacttg
    11581 gcacgcggca aactggttaa cgccggtgag gcagtcggtg tgattgctgc acaatccatt
    11641 ggcgaaccgg gtacccagtt gaccatgcgt acgttccaca tcggtggtgc ggcatcccgt
    11701 gcggcagcag ccagccaagt ggaagccaaa tccaacggta cggcacgatt cagcagccag
    11761 atgcgctacg ttgccaacaa caaaggcgag ttggttgtca tcggccgttc ttgtgaagtc
    11821 gtgattcacg acgatatcgg ccgtgaacgc gaacgccaca aagtacctta cggtgccatc
    11881 ctgctggtac aagacggtat ggccattaaa gccggtcaaa ccttggcaac ctgggatccg
    11941 catacccgtc cgatgattac cgaacacgca ggtatggtga aattcgaaaa cgtggaagag
    12001 ggcgttaccg ttgccaaaca aaccgatgat gtaaccggtt tgtccacttt ggtggtgatt
    12061 gacggtaaac gtcgttcctc tagtgcttcc aaactgctgc gtccgactgt gaaactcttg
    12121 gacgaaaacg gcgtggaaat ctgtattccc ggtacttcta ctccggtatc catggcattc
    12181 cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa tcggtaaagg cgacgtattg
    12241 gcgcgtattc cgcaagcctc ttccaaaacc cgcgacatta ccggcggcct gccgcgcgtt
    12301 gccgaattgt ttgaagcacg cgtgccgaaa gatgccggta tgttggcgga aattaccggt
    12361 accgtttcct tcggcaaaga gaccaaaggc aagcaacgtc tgattgttac tgacgtggac
    12421 ggtgtagcat acgagacctt gatttccaaa gagaaacaaa ttctggtaca cgacggtcaa
    12481 gtggtaaacc gcggtgaaac catcgtggac ggcgcggtcg atccgcacga tattctgcgt
    12541 ttgcaaggta tcgaagcact ggcacgctac attgtccaag aggtgcaaga ggtttaccgt
    12601 ctgcaaggtg tgaagatttc tgataaacac atcgaagtca tcatccgtca aatgttgcgc
    12661 cgtgtgaaca ttgcggatgc cggcgaaacc gggttcatta ccggagagca ggtcgaacgc
    12721 ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag gcaaagaacc ggcgcgttac
    12781 gaaaacgtat tgctgggtat taccaaagct tccctgtcca ccgacagctt catttctgcc
    12841 gcatcgttcc aagaaacgac ccgcgttctg accgaagccg cgattatggg caaacaagac
    12901 gagttgcgtg gtttgaaaga aaacgtcatc gtcggtcgct tgattcctgc cggtaccggt
    12961 ttgacttacc accgcagccg tcatcaacaa tggcaagagg tggaacagga gactgccgaa
    13021 acccaagtaa cggatgaata atctttggtg catccattca ataaaaaacc gcaagccttg
    13081 agcttgcggt ttttctttgt ccgattaagg caaaaacaag cgttttcgtc attttgaggc
    13141 gtgtggatta ttccttaggt attttcgggc cggagaccaa cgaggtggcg ggtgtcgtcg
    13201 gtacgtccgg agaccaaaat aactttgcca gggatgttgg tttcggcggt caaaaaaagt
    13261 agcgtcttaa tgttttccat ttaaacaaat gtcgtctgaa acttcagacg gcatttcctt
    13321 taagaaataa atatgaaacc cagaaatctc ttttttgcag gctgcctgct gacttcggcg
    13381 acgtttgccg aggatatcgg cgtacctgtc gaactgatta acgtcggtaa tcggattgcg
    13441 atgccgtctg aaggggaaag cctcgccctc ctgccgtttg ccgaggatgt accgccggtt
    13501 cgcgatgcaa tgccgtctga agttcctaaa agcgcggcag gcggcgatgt tcggggtgac
    13561 cggatgagaa tgccgattaa catcggatga gcgcggcttt atggcataaa aaactgtcgt
    13621 ggaaaggatt tacaccccaa ataaatttcc gttacaacaa gatcaacagc aatatgcccg
    13681 ccttttattc gcgcagcggc aaggaacggt ttgtcagtat agaaaaaacg tattgacagt
    13741 attttcttca gtcgtccgac tgattgtgag ggatgtcggt aaatatttat cggcaaacaa
    13801 gaaaatcatc tttcttcttg tcgttatgct tgactgtctg cttgcaataa aaatataatt
    13861 ccactcttgc cgacatggtg tcggcaagta tttaactcaa caggacgaga aaatatgcca
    13921 actatcaacc aattagtacg caaaggccgt caaaagcccg tgtacgtaaa caaagtgccc
    13981 gcactggaag cttgcccgca aaaacgtggc gtgtgcaccc gtgtatacac aactacccct
    14041 aaaaaaccta actctgcatt gcgtaaagta tgtaaagtcc gcctgaccaa cggttttgaa
    14101 gtcatttcat acatcggcgg cgaaggtcac aacctgcaag agcacagtgt cgtattgatt
    14161 cgcggcggtc gtgtaaaaga cttgccaggt gtgcgttacc acactgtacg cggttctttg
    14221 gatactgcag gtgttaaaga ccgtaaacaa gcccgttcca aatacggtgc taagcgtcct
    14281 aaataattac tgggacttaa ataggcacgt cggccgccta agctgaacaa cggccgagta
    14341 agtgaatact caattgggta ttcatgggaa tagacccgac tgaatagatt aaaggaaatt
    14401 aaaatgccaa gacgtagaga agtccccaag cgcgacgtac tgccagatcc taaattcggc
    14461 agcgtcgagt tgaccaaatt catgaacgta ttgatgattg acggtaaaaa atccgttgcc
    14521 gagcgtatcg tttacggtgc gttggaacag attgagaaaa aaaccggcaa agtagcaatc
    14581 gaagtattta acgaagccat tgcaaacgcc aaacctatcg tggaagtgaa aagccgccgt
    14641 gtaggtggtg caaactacca agttcctgtt gaagttcgtc cttcacgccg tttggctttg
    14701 gcaatgcgct gggttcgcga tgcggcccgc aaacgtggtg agaaatccat ggacctgcgt
    14761 ttggcaggcg aattgattga tgcgtccgaa ggccgtggcg gtgcgttgaa aaaacgtgaa
    14821 gaagtacacc gtatggctga agccaacaaa gcattctctc acttccgttt ctaattttga
    14881 aaggctaata aaatggctcg taagaccccg atcagcctgt accgtaacat cggtatttcc
    14941 gcccatattg acgcgggtaa aaccacgacg acagaacgta ttttgttcta taccggtttg
    15001 acccacaagc tgggcgaagt gcatgacggt gcggctacta ccgactacat ggaacaagag
    15061 caagagcgcg gtattaccat tacctccgct gccgttactt cctactggtc cggtatggcg
    15121 aaacaattcc ccgagcaccg cttcaacatc atcgacaccc cgggacacgt tgactttacc
    15181 gtagaggtag agcgttctat gcgtgtattg gacggcgcgg taatggttta ctgcgcggtg
    15241 ggcggtgttc aaccccaatc tgaaaccgta tggcggcaag ccaacaaata ccaagtgccg
    15301 cgcttggcgt ttgtcaataa aatggaccgt cagggtgcca acttcttccg tgttgtcgag
    15361 caaatgaaaa cccgtttgcg cgcaaaccct gtacctatcg tcattccggt tggtgcggaa
    15421 gacaacttca gcggtgtggt tgatttgttg aaaatgaaat ccatcatttg gaatgaagtc
    15481 gataaaggta caacctttac ctatggcgat attcctgccg aattggtcga aactgccgaa
    15541 gaatggcgtc aaaatatgat tgaagccgca gccgaagcca gcgaagaact gatggacaaa
    15601 tacttaggcg gcgacgagct gaccgaagaa gaaatcgtag gcgcgttgcg tcaacgtact
    15661 ttggcaggcg aaattcagcc tatgctgtgt ggttctgcat ttaaaaacaa aggtgttcaa
    15721 cgtatgttgg acgcagttgt agaattgctg ccagctccta ccgatattcc tccggttcaa
    15781 ggtgtcaacc cgaataccga ggaagccgac agccgtcaag ccagcgatga agagaaattc
    15841 tctgcattgg cgttcaaaat gttgaacgac aaatacgtcg gtcagctgac ctttatccgc
    15901 gtttactcag gcgtagtaaa atccggcgat accgtattga actccgtaaa aggcactcgc
    15961 gaacgtatcg gtcgtttggt acaaatgact gccgcagacc gtactgaaat cgaagaagta
    16021 cgcgccggcg acatcgcagc cgctattggt ctgaaagacg ttactaccgg tgaaaccttg
    16081 tgtgcggaaa gcgcgccgat tatcttggaa cgtatggaat tccccgagcc ggtaatccat
    16141 attgccgttg agccgaaaac caaagccgac caagagaaaa tgggtatcgc cctgaaccgc
    16201 ttggctaaag aagacccttc tttccgtgtc cgtacagacg aagaatccgg tcaaaccatt
    16261 atttccggta tgggtgagct gcacttggaa attattgttg accgtatgaa acgcgaattc
    16321 ggtgtggaag caaatatcgg tgcgcctcaa gtggcttacc gtgaaactat ccgcaaagcc
    16381 gttaaagccg aatacaaaca tgcaaaacaa tccggtggta aaggtcaata cggtcacgtt
    16441 gtgattgaaa tggaacctat ggaaccgggt ggtgaaggtt acgagtttat cgatgaaatt
    16501 aaaggtggtg tgattcctcg cgaatttatt ccgtctgtcg ataaaggtat ccgcgatacg
    16561 ttgcctaacg gtatcgttgc cggctatcct gtagttgacg tacgtatccg tctggtattc
    16621 ggttcttacc atgatgtcga ctcttcccaa ttggcatttg aattggctgc ttctcaagcg
    16681 tttaaagaag gtatgcgtca agcatctcct gccctgcttg agccaatcat ggcagttgaa
    16741 gtggaaaccc cggaagaata catgggcgac gtaatgggcg acttgaaccg ccgtcgcggt
    16801 gttgtattgg gtatggatga tgacggtatc ggcggtaaaa aagtccgtgc cgaagtacct
    16861 ttggcagaaa tgttcggtta ctcgaccgac ctgcgttctg caacccaagg ccgcgctact
    16921 tactctatgg agttcaagaa atattctgaa gctcctgccc acatagctgc tgctgtaact
    16981 gaagcccgta aaggctaatc agaaaaggcc gtctgaaact gaaaataaat tttcagacgg
    17041 ccattgttct ttaatcgatc tttatatgta aaggaattag ctcatggcta aggaaaaatt
    17101 tgaacgtagc aaaccgcacg taaacgttgg caccatcggt cacgttgacc atggtaaaac
    17161 cactctgact gctgctttga ctactatttt gtctaaaaaa ttcggtggcg ctgcaaaagc
    17221 ttatgaccaa atcgacaacg ctcctgaaga aaaagctcgt ggtattacca ttaatacctc
    17281 acacgtagaa tacgaaactg aaacccg
//
LOCUS       DAWISC010000024        17041 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWISC010000024 DAWISC010000000
VERSION     DAWISC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.024"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..518
                     /locus_tag="WIW87_000559"
     CDS             <1..518
                     /locus_tag="WIW87_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ5173757.1"
                     /translation="NRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNADELI
                     GNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVARSE
                     LQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR
                     VKKNDPSRFLN"
     gene            537..755
                     /locus_tag="WIW87_000560"
     CDS             537..755
                     /locus_tag="WIW87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173758.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            900..1244
                     /locus_tag="WIW87_000561"
     CDS             900..1244
                     /locus_tag="WIW87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ5173759.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            1244..1936
                     /locus_tag="WIW87_000562"
     CDS             1244..1936
                     /locus_tag="WIW87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ5173760.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            2003..3223
                     /gene="argJ"
                     /locus_tag="WIW87_000563"
     CDS             2003..3223
                     /gene="argJ"
                     /locus_tag="WIW87_000563"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ5173761.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            3283..4692
                     /locus_tag="WIW87_000564"
     CDS             3283..4692
                     /locus_tag="WIW87_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ5173762.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            4882..6273
                     /gene="hrpA"
                     /locus_tag="WIW87_000565"
     CDS             4882..6273
                     /gene="hrpA"
                     /locus_tag="WIW87_000565"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ5173763.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            6417..8060
                     /locus_tag="WIW87_000566"
     CDS             6417..8060
                     /locus_tag="WIW87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5173764.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            8104..8286
                     /locus_tag="WIW87_000567"
     CDS             8104..8286
                     /locus_tag="WIW87_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173765.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            8498..10075
                     /gene="lpt3"
                     /locus_tag="WIW87_000568"
     CDS             8498..10075
                     /gene="lpt3"
                     /locus_tag="WIW87_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ5173766.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            10177..13560
                     /locus_tag="WIW87_000569"
     CDS             10177..13560
                     /locus_tag="WIW87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ5173767.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQIARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDASAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFSATAANPLPNPLP
                     QEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQERE
                     QSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATSTLSDDSKPKKQPA
                     PQKNRLKPLPLADIRTFQAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMIREKIQLQIKALPK
                     QIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIFEQINQDEWAAFRL
                     PEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTAWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTLLAAGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLIKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(13593..14684)
                     /locus_tag="WIW87_000570"
     CDS             complement(13593..14684)
                     /locus_tag="WIW87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ5173768.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEVKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(14700..15344)
                     /locus_tag="WIW87_000571"
     CDS             complement(14700..15344)
                     /locus_tag="WIW87_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173769.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            15701..15853
                     /locus_tag="WIW87_000572"
     CDS             15701..15853
                     /locus_tag="WIW87_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173770.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            16223..16378
                     /locus_tag="WIW87_000573"
     CDS             16223..16378
                     /locus_tag="WIW87_000573"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ5173771.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(16468..16917)
                     /locus_tag="WIW87_000574"
     CDS             complement(16468..16917)
                     /locus_tag="WIW87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ5173772.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         4622 a         4630 c         3816 g         3973 t
ORIGIN      
        1 tcaaccgagt ccccgcccgg cgggcgggca atgccgacga actcatcggc aacgcgatgg
       61 ggcttaacga acagcccgtt ttacccgtca accgagcccc cgcccggcgg gcgggcaatg
      121 ccgacgaact catcggcaac gcgatgggac ttttgggtat tgcctaccgc tacggcggca
      181 catcggtttc taccggtttt gactgcagcg gcttcatgca gcacatcttt aaacgcgcca
      241 tgggcatcaa cctgccgcgc acgtcggcag aacaggcacg gatgggtacg ccggttgccc
      301 gaagcgaatt gcagcccgga gatatggtgt ttttccgcac gctcggcggc agccgcattt
      361 cccatgtcgg actttatatc ggcaacaacc gcttcatcca cgcgccgcgc acggggaaaa
      421 atatcgaaat caccagcctg agccacaaat attggagcgg caaatacgcg ttcgcccgcc
      481 gggtcaagaa aaacgacccg tcccgctttc tgaactgatt tcccaaggaa tacgcaatga
      541 gtatgcccga aatacccaaa tggtacgacg atgacggaca gatcgtgtcc tgtaccgaaa
      601 agatcaaagt gatgtccgaa aatatggccg agctgtatca gacggcacaa gacgcgtttg
      661 aagacgcgct gctgatgggt tgcggcgaac gtcagttgcg cgattacctg ctcgcgctga
      721 ttgaaggttt ggaaaatccc taccgcaaag tctgaacacg ccccggttgc tgcggcacgg
      781 tttatccgtg ccgtttttgc gtttgtgcgc ggcttcggct tttcagacgg catatttgac
      841 gttatgatta aacagttaac aagatttatc acaacgccgt caaaagacag acacacaaca
      901 tgaacatcat ccgcgcgctc ctcatcatcc tcggctgcct cgccgccggc gaaaccgccg
      961 ttttcctagc aggcatcaaa ctgcccggca gcatcgtcgg catgggcgtg ctgtttgcgc
     1021 ttttgcaggc aggttgggtc aaaacgtctt ggctacaaca gcttaccgac gcgctgatgg
     1081 cgaatctgac gctgtttctc gtgccgccct gcgtggcggt catcagctat ttggatttga
     1141 ttgccgacga ttggttttcg atactggttt ccgcctccgc cagcactttg tgcgtactgc
     1201 tggttacagg caaggttcac cgctggatac ggagcattat ctgatgaacg aaatcctcag
     1261 gcagcccagc gttctgcttt tcctcacgct tgccgtgtac gcgcttgcga ttatcgtgcg
     1321 cacgcgcacg ggcaatatct tctgcaaccc cgtactcgtc agcactatcg tgctgattgc
     1381 ctacctgaaa atcctcgaca tcgattatgc ggtgtaccac aacgccgcgc aattcattga
     1441 tttttggcta aaacccgccg tcgtcgtgct tgccgtgccg ctctaccaaa accgccgtaa
     1501 aatcttcaac cagtggctgc ccgtcatcgt ttcacagctt gcgggcagcg ttacgggcat
     1561 tgttacgggg atgtattttg ccaaatggct gggcgcggaa cgcgaagtcg tcctctcgct
     1621 cgcgtccaaa tctgttacca accccatcgc cattgaaatc acccgctcca tcggcggcat
     1681 tcccgccatt accgccgcca ccgtcatcat tgccggtctg gtcggacaga ttgccggtta
     1741 caaaatgctg aagaacacag tcgtcatgcc ctcgtccgtg ggtatgtcgc tcggcacggc
     1801 ttcgcacgcg atggggattg ccgcctcgct cgaacgcagc cgccgtatgg cggcatacgc
     1861 ggggctgggg ctgacgttca acggcgtact gaccgcgctg attgcgccgc tgctcatccc
     1921 cgttttggga ttttgaaccc gtttcagacg gcatttcagc ccatgctgtc tgaacgccga
     1981 cacactcgca aggagaaccg ttatggctgt caacctgacc gaaaaaaccg ccgaacaact
     2041 gcccgacatc gacggcattg ccctctacac cgcccaagca ggcgtgaaga agcccgggca
     2101 taccgacctg acactgattg ccgtagccgc cggcagcacc gtcggtgcag tcttcacgac
     2161 caaccgtttc tgtgccgcgc ccgtccacat cgccaaatcg caccttttca acgaagacgg
     2221 cgtgcgcgcc ctcatcatca acacgggcaa cgccaacgcg ggtacgggcg cacagggcag
     2281 aatcgatgct ttggcagtgt gtgccgccgc cgcccggcaa atcggctgca aaccgaacca
     2341 ggtgctgccc ttctccaccg gcgtgattct cgaaccgctg cccgcagaca aaatcatcgc
     2401 cgcgctgccc aaaatgcagc ctgccttctg gaacgaagcg gcacgcgcca tcatgaccac
     2461 cgacaccgtg cccaaagccg cctcgcgcga aggcaaggtc ggcgacaaac acaccgtccg
     2521 cgccacgggc atcgccaaag gctcgggcat gattcatccc aatatggcga ccatgctcgg
     2581 tttcatcgcc accgatgcca aagtttccca acccgtcctc caactgatga cgcaggaaat
     2641 cgccgacgaa accttcaaca ccatcaccgt tgacggcgac accagcacca acgacagctt
     2701 cgtcatcatc gccaccggca aaaacagcca aagcgaaatc gacaacatcg ccgacccgcg
     2761 ttacgcccaa ctcaaagaat tgttgtgcag cctcgcgctc gaactcgccc aagccatcgt
     2821 ccgcgacggc gaaggtgcga ccaagttcat caccgtccgc gtcgaaaacg ccaaaacccg
     2881 cgacgaagcc cgccaagccg cctacgccgt ggcacgttcg ccgctggtca aaaccgcctt
     2941 tttcgcctcc gaccccaacc tcggcaggct gctcgccgcc atcggttatg ccggcgttgc
     3001 cgacctcgat accgacctcg tggaaatgta tctcgacgat attttggttg ccgaacacgg
     3061 cggacgcgcc gcaagctaca ccgaagcaca agggcaggcg gtgatgtcga aggccgaaat
     3121 caccgtccgc atcaagctgc atcgcggaca agccgccgcc accgtctata cctgcgacct
     3181 gtcgcacgga tacgtttcca tcaacgccga ttaccgttcc tgacccgaca cggcttcaga
     3241 cggcatacat aaaatgccgt ctgaaccgcc ggacaacata ccatgacctc cacattcccc
     3301 cgccgcctcg cccgcaaaat ccgccaaacc cgccgcctgt cgcgcaaaag catcgccttt
     3361 ctgttccttt tggcaggttc ggcactcgtc gccctgaccg cgctgttttt tgcccatctt
     3421 gccgattttg cgctggaact gaacgccaaa ctggttcaac aatacccgtg gttcgcgtgg
     3481 gtcgcgcttc ctttgggttt accgcttatt gcgtggctca cacgcaaatt cgcccccttc
     3541 accgccggca gcggcatccc gcaggtcatc gcctcactgt cgctgcccta cggcgcacag
     3601 aaaacgcggc tgatccgcct cgggcagacg ctgctgaaga ttccgctaac ctttttgggt
     3661 atgctgttcg gcgcgtccat cggacgcgaa ggtccgtccg tgcaggtcgg cgcggcagtg
     3721 atgggcgcgt ggggcgcgtg gtgcaagaaa cacggcttgg cattcaaagg gatgcaggaa
     3781 aacgatttga tggcggcggg cgcggcgggc ggtttggcag ccgcgttcaa cgcgccgctg
     3841 gcgggcgtga ttttcgccat tgaggaactc gggcgcggca tcatgttgcg ctgggagagg
     3901 caaattcttt tgggcgtgct cgcctccggt ttcatacagg tcgccattca gggcaacaac
     3961 ccgtattttt ccggcttcaa cggcggcgta ttggaacata tctttctgtg ggtcgcactg
     4021 tccggcctgg tttgcggcgc ggcgggcggg ctgttcggac gtttgctcta tcgcggtgcg
     4081 gcggcgtttg caccgcgcaa gatacgcggc ttcatccgca accgtccgct gctgctggcg
     4141 gcactgatgg ggctgctgct cgccctgctc ggcacgttct accaaggcaa aacctacggc
     4201 accggctacc acgaagccgc ccaagccctg cacggcatct acgaagcccc cttcggactc
     4261 gccgccgcca aatggctcgc caccgtattc agctattggg caggcgttcc gggcggcatt
     4321 ttcactccct cgctgaccat aggcgcggtt ttgggcgagc atatcgccgc catcgccgac
     4381 atatcgcagg gtgcaaacat catcgtcctc atctgcatgg cggcatttct ggcgggcgcg
     4441 acacaatccc cgattacttc cgccgtcgtc gtcatggaaa tgacgggcgg acaaagcctg
     4501 ctgttttgga tgctaattgc ctgcattttc gcctcgcagg tttcgcgcca gttttcgccg
     4561 cgtccgttct accacgcatc gggaatgcgc ttccgccagc gcgtgcttca agaaaccgcc
     4621 gcccaaaccg gcaatgcgcc cgcaagaccg caaacagcaa acagcaaaac gggaatgccg
     4681 tctgaaaatt aaaacgcccc cgatcaaacg ccggcagccg ccttgatttg aataccgttc
     4741 cgccgccgct tgaaatttca gcaacaatgc cgtctgaacg acagaatgcg gttttcagac
     4801 ggcatttccc catcccgata ttgcctaaac aaaaccgaag cgtttgctat aattctattt
     4861 tttaccgcat acgcaccaat catgtttccc gatttctccc aaaccctctc caaagaccgc
     4921 cacttcctgc gttccgcctt caaaaatccc aacaaatacg gcggtttgtc caaaatcgaa
     4981 gaaaaatacc gaaaatcgca cgaaatcttt ttgaagcgtt tggcagcctt gccaaaaccc
     5041 gaattcgaca acaccctgcc cgttcacgag aagctcgaag aaatcaaaaa agccattgcc
     5101 aagaatcagg taacgattat ttgcggcgaa accggttcgg gcaaaaccac gcagttgccc
     5161 aagatttgct tggaactcgg gcgtggggcg gcaggattga tcgggcatac ccagccgcgc
     5221 cgtttggccg cgcgctccgt agcagagcgg attgccgaag agctgaaatc cgaaatcggc
     5281 agcgcggtcg gctataaagt acgcttcacc gaccacacct cgcgcgatgc ctgcgtcaag
     5341 ctgatgaccg acggcatcct gctggcggaa acgcagaccg accgttatct cgccgcctac
     5401 gacacgatta tcatcgacga agcgcacgag cgcagcctga acatcgactt ccttttgggc
     5461 tatttgaaac aactcctgcc gcgccgcccc gatttgaaag tcatcatcac ctcggcaacg
     5521 atagacgcag aacgcttctc ccgacacttc aacggcgcgc ccgttttaga agtgagcgga
     5581 cggacgtatc ccgtcgaaat cctctaccga ccgctgaccg gcaaagacga agacgacgca
     5641 gaagtggagt tgaccgacgc gattgtcgat gcggcggacg aattagcgcg acacggcgaa
     5701 ggcgatattt tggtattcct gccgggcgag cgcgaaatcc gcgaaactgc cgaagccctg
     5761 cgcaaatcca cgctgcgccg caacgacgaa atcctgcccc tgttcgcacg cctgtcgcac
     5821 gccgagcagc acaaaatctt ccacccctca ggcgcgaaac gccgcatcgt attggcaacc
     5881 aacgtcgccg aaacctcgct taccgtgccg ggcatcaaat acgtcatcga caccggcctc
     5941 gcgcgtgtta aacgctattc cgcacgggcg aaagtggagc agcttcatat cgaaaaaatc
     6001 tcccaagccg ccgcccgcca acgatccggc cgctgcggac gcgtctccgc aggcgtgtgt
     6061 atccgactgt tttcagaaga agattttaac agccgccccg aatttaccga ccccgaaatc
     6121 gtccgcagca acctcgccgc cgtcatcctg cgcatggcag cattgaaact cggcgatgtg
     6181 gcggcattcc cgtttttaga aatgcccgat tcacggtata tcaatgacgg ttttcaggtg
     6241 ttgttggagt tgggggcggt ggaggccgtc tgaaaacagg cagacataaa agaaaatccg
     6301 cgtagagtga tgtaaactta cccttgcttt aataagtaga aaatggtggg tttacgtccc
     6361 cccctgcggc tactaaaaaa atataagagt aaacaacctt tttgaaagaa aaatgtatgg
     6421 acgaaattca aatacccaaa aaagtggaat tacaaaccaa actagaaaat gaaaagattg
     6481 ttttatcgaa aggttctacc acgattattg ttggtgctaa tggcacaggg aaaacaagat
     6541 tagctgttta tattgaagaa caattaaagg aaaaagcaca cagaatttcg gctcatagag
     6601 cattaaaatt aaaccctaat gtcaataaaa taccagaaaa gagtgccaaa acatatctat
     6661 cttatggtca gaactgggat ggaatcgatg tatcaaatag aaaaaattat agatgggata
     6721 ataactcata tactcattta ctcaacgatt ttgattggtt attacaatat ttattcgctc
     6781 aacaaaataa tattgcggta gcaaataatc aaaagctcaa ccgtaatgaa aaagtaacca
     6841 attcaaaaac aaagctagat attttgcaag aagcatggga aacattatta ccacacagaa
     6901 aattacatat tacagcagat gatattcaag tctctgctgt agataatgag gaattgtatt
     6961 ctgcctcaaa tatgagtgat ggagagcgag cacttttcta tattcttgga caagttttgt
     7021 cagtagatga cggttctgtc ttaatttttg atgagcctga attacatatt cataaatcaa
     7081 ttatttcaaa tctatgggat aaaattgaag aattacgacc tgattgttca tttctaatca
     7141 ttacacacga tattgaattt gctgcaactc gagtagctaa aaaatatgtt atcagaaatt
     7201 attatccgac ccctgcttgg gatatttctg aagttcctga aagtaatttt gatgaagaaa
     7261 caataacgat gattttaggt agccgtaagc caatattatt tgttgagggc aacaataata
     7321 gtttagatat tgctacttac cgctattgtt atcctgattg gaccatcata cccaaagggg
     7381 catgcaaaga tgtcattcaa tcagtatcat cgctgaaaaa attaagtaat gaaatgccat
     7441 tactaaactt aaaatgttca ggtattgtcg atttagatag tagggatgaa agagaaattg
     7501 aacaattaaa taatttgggt atttacattt tacctgtatc cgaaattgaa aatcttttta
     7561 gcttaactga tgtagcaaaa gagatattga aactaaatca atattcagat gaagaattac
     7621 tcaataaact taatggattt aaatccgaac taattaaata tatagataat gaattaaaag
     7681 acgataaatt agacgaattt gttgtaaaac aggttcgacg taaaattgat aattatttaa
     7741 aaaatattga tttatcctcc aaaataacaa gtactgatat gaaaaaatca ttacttaatg
     7801 aaatttctac tttaacagaa cagaaaattg aaacatggat ttcagaaatt aaaaatgaaa
     7861 ttcaaagatg tattgaacag caagatttgg ataaattact tactatatat gataataaag
     7921 gactcttggc taaatcagct tgtgttttaa aaggaatgcg taacaaacat gaatttgaaa
     7981 gctggataat gagaacatta aaaggaagga ataaagattt tattgatgca atcagacaga
     8041 aacttccaat tctggattaa ataaaaccat ctgaaaattt accttcagat acagatatat
     8101 ttcatgaaaa atcatcaaac tacactctct ttccctactt cgagtagcct gaaaccttgc
     8161 gcagacaaac aaggcctgtc tgaagaccgc agccaatacc gcctgaccaa actcggcgaa
     8221 caaatggcgc acctgcctat cgacccgaaa attgcgcgta ttttgttagt attattccgt
     8281 ttttaaaaat gcccgattcg cggtatatca atgacggttt tcaggtattg ctggaattgg
     8341 gggcggtgga ggccgtctga aaataaaatc tttctttata aaaaggcagg ccatgtttca
     8401 ttttcagacg gcctaaatca ttgagaaact aaaaactatt aaaaagggaa tattgggttt
     8461 taaaactcaa tcggtaaatt tttattgtga aatattaatg atgaaaaaat ctttccttac
     8521 gcttgttctg tattcgtctt tacttaccgc cagcgaaatt gcctatcgct ttgtatttgg
     8581 gattgaaacc ttaccggcgg caaaaattgc ggaaacgttt gcgctgacat ttgtgattgc
     8641 tgcgctgtat ctgtttgcgc gttataaggt gacgcgtttg ttgattgcgg tgttttttgc
     8701 gttcagcatt attgccaaca atgtgcatta cgcggtttat caaagctgga tgacgggcat
     8761 caattattgg ctgatgctga aagaggttac cgaagtcggc agcgcgggtg cgtcgatgtt
     8821 ggataagttg tggctgcctg tgttgtgggg cgtgttggaa gtcatgttgt tttgcagcct
     8881 tgccaagttc cgccgtaaga cgcatttttc tgccgatata ctgtttgcct tcctaatgct
     8941 gatgattttc gtgcgttcgt tcgacacgaa acaagagcac ggtatttcgc ccaaaccgac
     9001 atacagccgc atcaaagcca attatttcag cttcggttat tttgtcggac gcgtgttgcc
     9061 gtatcagttg tttgatttaa gcaggattcc cgcctttaag cagcctgctc caagcaaaat
     9121 cgggcagggc agtgttcaaa atatcgtcct gattatgggc gaaagcgaaa gcgcggcgca
     9181 tttgaagctg tttggctacg gacgcgaaac ttcgccgttt ttaacccggc tgtcgcaagc
     9241 cgattttaag ccgattgtga aacaaagtta ttccgcaggc tttatgactg cagtgtccct
     9301 gcccagtttt ttcaatgcga taccgcacgc caacggcttg gaacaaatca gcggcggcga
     9361 taccaatatg ttccgcctcg ccaaagagca gggctatgaa acgtattttt acagcgcgca
     9421 ggcggaaaac gagatggcga ttttgaactt aatcggtaag aaatggatag accatctgat
     9481 tcagccgacg caacttggct acggcaacgg cgacaatatg cccgatgaga agctgctgcc
     9541 gttgttcgac aaaatcaatt tgcagcaggg caagcatttt atcgtgttgc accaacgcgg
     9601 ttcgcacgcc ccatacggcg cattgttgca gcctcaagat aaagtattcg gcgaagccga
     9661 tattgtggat aagtacgaca acaccatcca caaaaccgac caaatgattc aaaccgtatt
     9721 cgagcagctg caaaagcagc ctgacggcaa ctggctgttt gcctatacct ccgatcatgg
     9781 ccagtatgtt cgccaagata tctacaatca aggcacggtg cagcccgaca gctatctcgt
     9841 gccgctagtg ttgtacagcc cggataaggc cgtgcaacag gctgccaacc aggcttttgc
     9901 gccttgcgag attgccttcc atcagcagct ttcaacgttc ctgattcaca cgttgggcta
     9961 cgatatgccg gtttcaggtt gtcgcgaagg ctcggtaacg ggcaacctga ttacgggtga
    10021 tgcaggcagc ttgaacattc gcgacggcaa ggcggaatat gtttatccgc aatgagtggc
    10081 gtaaaaacca ataaagacaa atttagatga tgtcggggaa gatgcccgac cgacaagact
    10141 atgcaaaata tgaaaaacca agtacgcgga tcaggcatgg atgcccgatc caatccggcc
    10201 aatgtttcag acggcctgca aaacagttcg ggtcatatcg gtaccaacac gcgttaccgc
    10261 ctgaccaaac tcggcgaaca gatagcgcgc ctacccatcg acccgaaaat cgcgcgcatt
    10321 ttgctggcgg cgaagaaaca cgactgcatg gcggaaatat tggtgattgc gtccgcgctg
    10381 tcgattcaag acccgcgcga gcggccgcta gaagcgcgcg atgcctcagc caaggcgcac
    10441 gagcgtttta ccgacaagca gtccgatttc cttgcctatc tgaacatttg ggacagcttc
    10501 cagcgcgaac gcgataaagg cttgtccaac aagcagctgg tgcagtggtg ccgccaatat
    10561 ttcctgtcgc acctgcggat gcgcgagtgg cgcgagctgc accaccagct tgcccaaacc
    10621 gcgattgaaa tgggtttaac caccaaggaa gccgctttca gacgacctcc cgaagtcagg
    10681 cagctcacgt cgtctgaaaa tgcgggtgac caagacctat ctgctaaact caaacaaaaa
    10741 caactggata aaaagcaaca ccgcgcccaa atccgcgccg ccaaagaagc gggctacgaa
    10801 caaatccacc gcgccctgct cactggcctt atcgccaacg tcggcatgaa atcgcccgac
    10861 ggtaacgact acaccggcgc gcgcggcagc cgcttccacc ttttccccgc ctccgccctg
    10921 ttcaaagcca aacccaaatg ggtgatggcg gcagaattgg ttgaaaccac gcgcctttac
    10981 gcgcgcgacg tcgccgttat ccagcccgaa tggatagagc aggaagcgcc gcacctcgtc
    11041 cgctatcatt atttcgagcc gcattgggaa caaaaacgcg gcgaagtcgt cgccagcgaa
    11101 cgcgtgacgc tttacggtct gaccgtattg ccgcgccgcc ccgtgtctta cggcaaagtt
    11161 gcccccgaag aagcgcgcga aatctttatc cgcagcgcgt tggtggcgca ggaatgcgat
    11221 ttgaaagcgg atttttttgt ccacaacaaa aagctgatta aagaaattac cgaactcgaa
    11281 cacaaatcgc gcaagcaaga cgtgctggtc gatgacgaag ccctgtttgc gttttataac
    11341 gaacgactgc ccgaaatggc ttggaaagac gcgcaaggca gcgtttgggg aagtgaagat
    11401 tccgtacgga ttattgaatc tgacaaagcc gagaggtcgt ctgaaaatga gcgcaacgag
    11461 tttcgtaaaa acaagcgtaa tgggtctcgc caaaatgaaa atcacggcaa caccgtaggt
    11521 tgggttgaaa acccaacatc agccgcaact gcaaaaactg ttgggtttga caatccaacc
    11581 tacgctgccc aacaaaccac cccctccccc gtgggggagg gtcggggaga gggcaaaaca
    11641 gttgccgcac aaaccaactt ttccgcaacc gcagcaaacc ctctccctaa ccctctcccg
    11701 caggagaggg aacagagtgc cgcagcttca acgatttcag acgacctgcg tcctgcaaat
    11761 ctgcagcaaa ccgccccctc ccccgtgggg gagggctggg gagagggcaa aacagttgcc
    11821 acacaaacca acttttccgc aacctcaaca aaccctctcc cgcaggagag ggaacagagt
    11881 gcctcagctt caacgttttc agacgacctg cgtcctgcaa atctgcagca accctctccc
    11941 tcccccgtgg gggagggctg gggagagggc aaaacagttg ccacacaaac caacttttcc
    12001 gcaacctcaa cactttcaga cgactccaaa cccaaaaagc agcctgcacc ccaaaaaaac
    12061 cgtctgaaac ccctacccct cgccgacatc cgcaccttcc aagcctggct caaaaccgcc
    12121 gagcgcgaca atccgcgcct gctgttcctc agccgcgacg atctgatgca acacgccgcc
    12181 gcacacatta ccgaagaaca gttccccaaa ttctggcaaa ccgcagacgg caaattcaaa
    12241 ctttcctacc gcttcgagcc gcaccatccg ctagacggcg tgaccatgac cgtgccgctg
    12301 accgtcctca accgcctgca cgcgccgtcg ctcgaatggc tggtgcccgg catgatacgc
    12361 gaaaaaatcc agttgcaaat caaagcactg cccaagcaaa tccgccgcat ctgcgtgccc
    12421 gtgcccgaat tcatcaccca atttttaagc caaaaccccg accgcaacgc ccccatcctg
    12481 ccccaactcg cccaagccat cgccaaaacc gcaggcgaca tccgcatatt cgagcaaatc
    12541 aaccaagacg aatgggccgc gttcaggctg cccgaacact gctatttcaa cctccgcatt
    12601 atcgacgacg gcggacaaga gcttgccggc ggccgcaaac tgcacgaatt gcaacaacaa
    12661 ctcggtcaag ctgccgccgt taccttccgt gacaacaccc aagaatttga gcgcgacaac
    12721 gtcaccgcat gggacatcgg caccctgccc gaatccatca aattcgcacg cggcaaacaa
    12781 cagctcaccg gctatctcgg cctacaaaaa gaaaaagacg gccgcatcgc cctgcgcctg
    12841 tttgatacca cagaagccgc agagcaggca caccgtcaag gtgtcatcga attgatgaag
    12901 ctgcaattaa aagagcaggt aaaggatttg aacaaaggca tccaaggctt cacccaagct
    12961 gccatgctgc tcaaacacat caacgccgac actctgcgcg acgacctcac ccaagccgtc
    13021 tgcgaccgcg cctttatcgg cgaagacgag ctgccgcgca acgaaaaagc cttcaaagaa
    13081 caaatcaaac gcgcccgcag ccgcctgccc gccgtcaaag aagccctcag ccgctacctg
    13141 caggaaaccg ccgccgtcta cgccgaactc aacagcaaac tcggcaaaca cccattgacc
    13201 caccttctaa gactacgcct gcaaaccctg ctcgccgccg gcttcgccac ccgaaccccg
    13261 tgggcacaat ggccgcgcct ccccatctac ctcaaagcca tgaccctgcg cctcgaaaaa
    13321 tacagcagca accccgcccg cgacgcagcc cgcgaagccg atatccaaga gctggaacaa
    13381 atgtggcagg aaaaaacaga cagcctgatt aaacaaggtc tccccatttc agacggcctc
    13441 gccgcgttta aatggatgat tgaagaattg agggtgtcgc tgttcgcgca ggaattgaag
    13501 acaccgtatc cggtgtcggt gaagcggctg ttgaaagagt gggaaaaaat tgaaaaataa
    13561 aaaaacagcc tgaaaagttt caggctgttt ttttatttga ctaatcgaag tttcctatat
    13621 ctatttaagt ccctctcaac taatccaaaa gttaaatcag caacatcttt gggggatacg
    13681 tttaaatttt cagcaatctg ttcaatacca atgccatcat tttttaaaat agtaagcatt
    13741 ttacgtaatg cgcttgatat ttccctttcc attggctctg gttcgatagt tcgatatttt
    13801 ttctttgcaa acaaaggaca aagattgtgt atatacatcc tatcagtaat cattcctaat
    13861 ttatgcatcc gatatgctaa ggcaacaagt gatacaccaa atcgtctttt gatttttaat
    13921 aaattttcaa tagtgatagg aacatgacga tataagcgta gtgcagcctc cggcattaaa
    13981 aaagctgaag caaaggcatt agcctctttt tcgataatat cacgaggttc atcttctgta
    14041 atttcactat ttttactatg ttccatactg tatttatcac ggattaagtg ccctaattca
    14101 tgggcagcat caaatcgact acgttctgca gatttttgtg tatttaaaaa tacaaatgga
    14161 tgattttcat accaagtaca aaaggcatca atgtcctttg tatctaaaga taatgaaaat
    14221 acacgaacac ccttaacttc aagtagggtg atcatattcg gaataggttc attgccaagc
    14281 ccccattcta atcttagttc ctgagcagcc tcttcaggag aaatatcaga aaaatcaggc
    14341 aatacggctt gacttagtgt aaattctgtc tcgagccagt catttaacaa aaaagccgta
    14401 atgctatgat ttaatgcttg tttttcaagc ctcttcgagg tgcgtgaacg agcacgaaaa
    14461 cttactgcct gagatttcaa ctcaggcagt ctttcgtcat tagtaaagaa atgaactgga
    14521 aactctaata aattggctaa ttcatttaaa tcaggtattt gctcatcttt tacatagttt
    14581 ctaacctgtc gagcggtaat acctaataac tcagctaatt ttgtttgcgt acaaccacgt
    14641 ttatccagcg caaattccag tctctcacga ttaaatgtct gcatgattta tcattcaaat
    14701 tatcctgctt tttgttttct ttcaaccaaa ggctcatatt cttcaacagg ttgcttacgt
    14761 tcaagctcat caaatttagt taaatcaaca tcagctaata taattcgctg cttgtaccca
    14821 gttatttgat gactaacaaa accactcggt aaagataatt caagttgcac tttattatac
    14881 ttccagtgaa acagcagaac ccaaaactgc acagtatcag gcaaatctag ttttgaatta
    14941 cgaatagcct cctcaaatcc cttacctttc cttgcggttg tcattggcat cccgtgatgc
    15001 ctaccaacat ctgaagtagc agtagccaca ataatacttt tagttcgaca tggcgagaga
    15061 catagaaatg caccacccga ccaaggctca agcgtccagc catctttact taaatatacc
    15121 cttagagcaa atgtaatttc tgcttgccga tacatcccca atgtatttct gtcagataat
    15181 gctgctttgt cctgaatatt attatgcgca gtaagtacaa tctctttaag catctcctga
    15241 gataagtact tgctgatttc acttaaagca atatcactat tttgttgctc gactatttct
    15301 cctacttcaa atgggaaagg ttctgataat gcaaattcca ccataaaaat ttcctaattt
    15361 tatacgtaat gtttacacaa tatatcagga aatatgaaaa cgtacaacta tatctataaa
    15421 gcaattaata agtagcctgc ccaaccgtgt ccttatcttt cggcacaccc gacctgcaaa
    15481 tcacgcaaaa cttggaatcc gtgtgtaggg tgtgtgcggt acatacgcac gcagtctttt
    15541 taaaccacag cccttcccaa ctaaaccaaa aggtcgtctg aaccctattt tcagacgacc
    15601 ttttgccact ttgtaaaaca aatcttccca ccatcctctc cccaaacatc gcccgaacca
    15661 gtaaacttct catatttcaa caactccttg gaagcaaacc atgtctggta tctacctacc
    15721 ccgcctattc ccgccccata tcgccgaacg cggcctgttg tattttcagc agggcaaggt
    15781 tctcgatgtc cgaaaaactt ccgccgggca ttatcgggcg gaggtgtgcg gttcggaaaa
    15841 ctattgggta tagttgaagc tggatagtga tttgtatatt aaagacgaag gctgcaattg
    15901 tccttatatc taagagtgca aacatacctt aaattactat attgcatagg caaaatacaa
    15961 gcctataacg aattggaaac aaaatgccgt ctgaaaacat cttcagacgg cattataaaa
    16021 tctgttcacc ttttcagatg agtaatgtac acccttatac aatttttgct actatgcccc
    16081 ataaatccac ggctaaagat atccttatta tgtcctatga tttatcgaaa cgacttgtaa
    16141 tcggcttagc atcaagtgcc ctattcgact tatccgaatc ggataatata tttagaatgg
    16201 aaggggcaga aacctatagg caatatcaga gagaaaaaca aaaccatccc ctaaaaaagg
    16261 cgttgtcttt ccatttatta aaaaacttct gtcaatcaat gaaataaacc caaacgaccc
    16321 aacgattggg tttattcttt tatccagaaa caatccagat acagattacg agtcataact
    16381 ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac aaatactgaa
    16441 acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct tatcatttcc
    16501 tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa taaaggttac
    16561 tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg ctaccgcctt
    16621 caacatgaat ttactgtcta aagccccgcg cgcgattcca ttcaaacgga tacaaaagcc
    16681 ttctgcctct ttaatcggca aacttggcca cttggtagat gtttgtttaa acctcccatt
    16741 ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc gcactgcatt
    16801 tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag caagtacaga
    16861 caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat cagtcattac
    16921 tttacttcca ataagaacag attattcaac atatttcttt gaacagactt actatcccat
    16981 tcaacagtat gcatatttcc cactctattt tttagcggcc ggtatagccg gtttggctgg
    17041 g
//
LOCUS       DAWISC010000025        16922 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWISC010000025 DAWISC010000000
VERSION     DAWISC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.025"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(51..1220)
                     /locus_tag="WIW87_000575"
     CDS             complement(51..1220)
                     /locus_tag="WIW87_000575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ5173773.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEHIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="WIW87_000576"
     CDS             1566..2462
                     /locus_tag="WIW87_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ5173774.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WIW87_000577"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WIW87_000577"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ5173775.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLLYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WIW87_000578"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WIW87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ5173776.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGDIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="WIW87_000579"
     CDS             complement(4867..6819)
                     /locus_tag="WIW87_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ5173777.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="WIW87_000580"
     CDS             complement(7096..7719)
                     /locus_tag="WIW87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ5173778.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /locus_tag="WIW87_000581"
     CDS             7925..8554
                     /locus_tag="WIW87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ5173779.1"
                     /translation="MNLFQNAKFFTTINHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWLDAADAAASSPEEN"
     gene            complement(8675..11071)
                     /locus_tag="WIW87_000582"
     CDS             complement(8675..11071)
                     /locus_tag="WIW87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ5173780.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /locus_tag="WIW87_000583"
     CDS             11223..12338
                     /locus_tag="WIW87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ5173781.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIVSVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="WIW87_000584"
     CDS             12341..12940
                     /locus_tag="WIW87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ5173782.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /locus_tag="WIW87_000585"
     CDS             12941..13588
                     /locus_tag="WIW87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ5173783.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="WIW87_000586"
     CDS             13606..14151
                     /locus_tag="WIW87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ5173784.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16479
                     /locus_tag="WIW87_000587"
     CDS             14170..16479
                     /locus_tag="WIW87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ5173785.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQQ
                     AAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRS
                     LDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESG
                     GVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
                     QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKY
                     KNVEEFRSILRLDNADTTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDE
                     LDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKW
                     GAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAK
                     IESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQC
                     ASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFK
                     TRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4567 a         4808 c         3867 g         3680 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggcaat cagtttttct
      241 tcgctgattt tgccggtgcc gaaagtatcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaaac ctgcggcgac gatgtttttt
      361 gcgacatagc ggcaggcgta agcggcggaa cggtccactt tggacgggtc tttgccggag
      421 aatgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg ataacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcagaaccg gtttgatgat gtgttcgatt
      601 acggcgtttt tcagctcttc gtaagcgatg gacggatcgt gctgggtaga caggacgacg
      661 gtgtcgatgc gttttacttt gccggtttcg ctgtcgtaaa ccacggtcag ttgggctttg
      721 gcatcaggac gcagccaagg caggcggccg tctttgcgca attcgctttg acgctgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtag gggtttcgtc acaggcatag
      841 ccgaacatca aaccttggtc gcccgcgccc tggttcaagt cgatgccttc gccttcgttc
      901 acgccttggg cgatgtcggg ggattgctgg tcgtagtaca cgccgactgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ctacttgggc ggtggtggta atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaagg tttctgcggc gacacgtgct tttgggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg atacttggtc ggcaacttta tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaata gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cacgcatcgt agcagaaaag gcatattgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcaggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccgct ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatcta gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg gcaggcgggt ttgaaccccg accccaaaac ggtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg
     1921 aagggctgct attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag
     2041 acaaaatcat gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc
     2341 aaggggaatt gaacggcgac aaagcccatg atgccgccgt gttcaaccgc aatgccgaat
     2401 attggatacg ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatc tgacgatttc
     2521 cgacagcggc cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg
     2581 catcgcaccg gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct ttgggggtcg ggatttgcac
     2701 cgtcatgttg ccgaaagttt cacggagctt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 taccgcatct tgaaacgcac ggcagatgtc gttgcgtgtc tgctcaggaa tgtcatccgc
     2881 cccgttttcc gcgcgcactt tctcgacggc ggtcaatacg gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgaacct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacagcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca acagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg
     3241 atggacggga ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc
     3301 cgccagcagc gcgccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgcg cctgcctgcg ccagacagcc ttccgtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccaattccg gcacaacccc gccgtattcg gcgtgcattg ccatttgagt
     3481 gtgcaggcag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacac ctgtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatggtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccagga ctggagcgcg
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataggcgaac
     4501 agggcgaaaa agacaaaggc gcactgccac atgatcgccg actggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg tcgcctccgt acaaaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcacttgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcgctgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cgattccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat agccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg cctttggttg cgtcggcaac
     5461 cagacgtttg cgcccttccc catctttcaa aaactcacgc aatgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtgaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttgtcc gtcacttcaa tatcgctggc
     6061 gaaatcacgc ggcatacccg tattgtaaaa atcgatatgg aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatagtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagaggcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgtaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg atcaaccacc ttaaagacct
     7981 gcccgacacc cctctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggattt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt tggacgcagc
     8521 agatgccgcc gcttcctctc cagaggaaaa ctgaccccaa ttatacggaa accgtattcc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaaagtct ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccagc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccaaac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaaaccca aggcgcgcct gtcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaacaca acggcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aacgcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc tggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttatcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggcagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgtgtccg
    11521 tcccgcaaaa tttggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttca
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcggcg cgccaggaag
    12181 gcatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa atcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaatc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggtct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcagc gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag ggccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtccggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctaatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcctctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctgccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccga accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 tcagcaaaac aacaggcggc agcaccagca aaacaacagg cggcagcacc agcaaaacaa
    14821 caggcggcag caccagcaaa acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc
    14881 ggcattatcg aattggctgc attgggcttt gccgggcagc ccgacatcag ccaacagcac
    14941 gaccacatca tcgttacgct gaaaaaccat accctgccga ccacgctcca acgcagtttg
    15001 gatgtggcag actttaaaac accggttcaa aaggttacgc tgaaacgcct caataacgac
    15061 acccagctga ttatcacaac agccggcaac tgggaactcg tcaacaaatc cgccgcgccc
    15121 ggatacttta ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac
    15181 aatgcgccca aaaccttcac aggccggaaa atctcccttg acttccaaga tgtcgaaatc
    15241 cgcaccatcc tgcagatttt ggcaaaagaa tccggaatga acattgttgc cagcgactcc
    15301 gtcaacggca aaatgaccct ctccctcaag gatgtgcctt gggatcaggc tttggatttg
    15361 gttatgcagg cgcgcaacct cgatatgcgc cagcaaggga atatcgtcaa catcgcgccc
    15421 cgcgacgagc tgcttgccaa agacaaagcc ctcttacagg cagaaaaaga cattgccgat
    15481 ttgggtgcgc tgtattccca aaacttccag ttgaaataca aaaatgtgga agaattccgc
    15541 agcatcctgc gtttggacaa tgccgacacg accggaaacc gcaacacgct tatcagcggc
    15601 aggggcagcg tgctgatcga tcccgccacc aacaccctga ttgttaccga cacccgcagc
    15661 gtcatcgaaa aattccgcaa actgattgac gaattggacg tacccgcgca acaagtgatg
    15721 attgaggcgc gtatcgtcga agcggcagac ggcttctcgc gcgatttggg cgttaaattc
    15781 ggcgcgacag gcaagaaaaa gctgaaaaat gatacaagcg cattcggctg gggggtaaac
    15841 tccggcttcg gcggcgacga taaatggggg gccgaaacca aaatcaacct gccgattacc
    15901 gctgccgcaa acagcatttc gctggtgcgc gcgatttcct ccggtgcctt gaatttggaa
    15961 ttgtccgcat ccgaatcgct ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc
    16021 caaaaccgca aagaggccaa aatcgaatcc ggttacgaaa ttcctttcac cgtaacctca
    16081 atcgcgaacg gcggcagcag cacgaacacg gaactcaaaa aagccgtctt ggggctgacc
    16141 gttacgccga acatcacgcc cgacggccaa atcattatga ccgtcaaaat caacaaggac
    16201 tcgcctgcgc aatgtgcctc cggtaatcag acgatcctgt gtatttcgac caaaaacctg
    16261 aatacgcagg ctatggttga aaacggcggc acattgattg tcggcggtat ttatgaagaa
    16321 gacaacggca atacgctgac caaagtcccc ctgttgggcg acatccccgt tatcggcaac
    16381 ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt cattaccccg
    16441 aggattatgg gtacggccgg caacagcctg cgctattgat gcgtcaaaat aagggcatat
    16501 gttttacggc atatgccctt tctttatgct ttttgccgcg accgaaatgc cgtcattccc
    16561 gcgcaggcgg gaatccagtc cgttcagttt cggtcagttt cggtcatttc cgataaattc
    16621 ctgttgcttt tcatttctgg attcccactt ttgtgggaat gacggcggaa ggggtaaatc
    16681 ctcacaaccc aaagcctcgt catttccaca aaaaacagca acccgaaaca gcaacttaaa
    16741 accccgtcat tcccgcgcag gcgggaatct aggtctgtcg gttcaggaac ttatcggata
    16801 aaacggtttc tccaaccctg cgttctagat tcccactttc gtgggaatga cgggatatgg
    16861 gtttccgtgc ggacgtgttc ggatttccgc ctgcgcggga atgacggcga cagatgccca
    16921 ac
//
LOCUS       DAWISC010000026        16868 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWISC010000026 DAWISC010000000
VERSION     DAWISC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.026"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WIW87_000588"
     CDS             complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WIW87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ5173786.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1916..2050
                     /gene="rpmH"
                     /locus_tag="WIW87_000589"
     CDS             1916..2050
                     /gene="rpmH"
                     /locus_tag="WIW87_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ5173787.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2053..2418
                     /locus_tag="WIW87_000590"
     CDS             2053..2418
                     /locus_tag="WIW87_000590"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ5173788.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2483..2704
                     /gene="yidD"
                     /locus_tag="WIW87_000591"
     CDS             2483..2704
                     /gene="yidD"
                     /locus_tag="WIW87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ5173789.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2876..4513
                     /gene="yidC"
                     /locus_tag="WIW87_000592"
     CDS             2876..4513
                     /gene="yidC"
                     /locus_tag="WIW87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ5173790.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4594..5304)
                     /locus_tag="WIW87_000593"
     CDS             complement(4594..5304)
                     /locus_tag="WIW87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ5173791.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQEIISQTIVQWRKR
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(5366..5956)
                     /gene="maf"
                     /locus_tag="WIW87_000594"
     CDS             complement(5366..5956)
                     /gene="maf"
                     /locus_tag="WIW87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ5173792.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6104..6607
                     /locus_tag="WIW87_000595"
     CDS             6104..6607
                     /locus_tag="WIW87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ5173793.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGSNGTPEKADRDKPNPFAV
                     LAGLKSN"
     gene            6641..6820
                     /gene="rpmF"
                     /locus_tag="WIW87_000596"
     CDS             6641..6820
                     /gene="rpmF"
                     /locus_tag="WIW87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ5173794.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6995..7522
                     /locus_tag="WIW87_000597"
     CDS             6995..7522
                     /locus_tag="WIW87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ5173795.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7611..8666
                     /gene="plsX"
                     /locus_tag="WIW87_000598"
     CDS             7611..8666
                     /gene="plsX"
                     /locus_tag="WIW87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ5173796.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAVLPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            8802..9053
                     /locus_tag="WIW87_000599"
     CDS             8802..9053
                     /locus_tag="WIW87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ5173797.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9080..9496
                     /locus_tag="WIW87_000600"
     CDS             9080..9496
                     /locus_tag="WIW87_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173798.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            9516..9719
                     /locus_tag="WIW87_000601"
     CDS             9516..9719
                     /locus_tag="WIW87_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173799.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            9746..10708
                     /locus_tag="WIW87_000602"
     CDS             9746..10708
                     /locus_tag="WIW87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ5173800.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10755..11126
                     /locus_tag="WIW87_000603"
     CDS             10755..11126
                     /locus_tag="WIW87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ5173801.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYSID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            11152..12078
                     /gene="fabD"
                     /locus_tag="WIW87_000604"
     CDS             11152..12078
                     /gene="fabD"
                     /locus_tag="WIW87_000604"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ5173802.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            12217..14082
                     /gene="msbA"
                     /locus_tag="WIW87_000605"
     CDS             12217..14082
                     /gene="msbA"
                     /locus_tag="WIW87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ5173803.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            14182..15747
                     /gene="guaA"
                     /locus_tag="WIW87_000606"
     CDS             14182..15747
                     /gene="guaA"
                     /locus_tag="WIW87_000606"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ5173804.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            15809..16555
                     /gene="fabG"
                     /locus_tag="WIW87_000607"
     CDS             15809..16555
                     /gene="fabG"
                     /locus_tag="WIW87_000607"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ5173805.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4269 a         5147 c         3977 g         3475 t
ORIGIN      
        1 ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg tctgaaaacg
       61 ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc aattccggat
      121 cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgcc
      181 caccaaacga atcgccgata gacggcaggc tcaaagtagt cagttctttg gtcaggctca
      241 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
      301 ttttgatgcg gtaatatttc gccaccgcat cgatgatgat gtcggcggtg atgactttgt
      361 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
      421 ggttcataaa gcggctgctc gctccgacac gattaaacgc gccttcaagc tcgcgcacgt
      481 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcgatactg atgcccgccg
      541 cttccgcctt tttctgcaaa atggcgatgc gcatttccaa ttcgggcggc tcgagttcca
      601 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc catgccttcg attttcgcag
      661 gcaacacatc gcaagtgagg atgagctgtt ttttctcgtt gtggaaatgg ttgtacagat
      721 agaaaaactc ttccatcgta cggtctttgc ctttgatgaa ctggatgtcg tcgataatca
      781 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta agtgttgttg cgaaccgcct
      841 tcataaagct gcggatatag tcatccgaat gcatatagcg cactttggca tcgggacggt
      901 ttttcagcag ctcgttgccg accgcctgca caaggtgggt tttgcccaaa cccgtgctgc
      961 catagaggaa gaacgggttg taactctgcc ccgggctttc cgcaatcgcc tgcgccgcag
     1021 ccgccgcaag gcggttgccc ttaccttcta ccaacgtatc aaacgtgtaa tccggagaca
     1081 ggttggtctg ctcgtaacgc gcctcttccg catcgcgctg cacgtccgtc cgtgctttgg
     1141 caactgccac cgattccggc cgggaagcag acccggcagc ctgacgcggc tcgtgcggca
     1201 ggtttttcat acgttccgcc aaaatatccg ccgccgtttt cgacgcagcg ggtttgacag
     1261 gctcttcaga cggcagctcg tccaacagaa cctcctgcac gggcattccc tccgacaccg
     1321 catgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgca
     1381 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     1441 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg ttcttgccgt
     1501 acaccaccca tacgccaccc tcctcaccaa cggtaagggg cgcaatccat tgcgcaaact
     1561 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     1621 tcatggatag gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     1681 aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc cgattatacc
     1741 gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa ggcggcggaa
     1801 atccgcaggc aatcgggcaa tcttcctgca aagtttctat attgattgac aaaagcggca
     1861 aattggagtg taattcacgg tttaattatc tacccattct attttaggaa acatcatgaa
     1921 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     1981 caaaacgcgc ggcggccgcg cagtattggc cgcacgccgt gccaaaggcc gcaaacgcct
     2041 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
     2101 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgc
     2161 tcaaacggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     2221 aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt tagattgaac
     2281 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacagggct
     2341 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     2401 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac ggcaacgggt
     2461 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
     2521 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     2581 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     2641 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgttcc
     2701 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     2761 aatgccgtct gaagcttcgg gcaggcggca cgaccgccgg gtatgaagcc cgcccttatt
     2821 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
     2881 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
     2941 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     3001 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     3061 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     3121 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     3181 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     3241 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     3301 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     3361 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     3421 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggttact ttacccactc
     3481 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttttccga
     3541 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     3601 cggctggctc ggcatgattg aacaccactt catgtccacc tggattctcc aacctaaagg
     3661 cagacaaagc gtttgcgccg caggcgagtg caacatcgac atcaaacgcc gcaacgacaa
     3721 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
     3781 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
     3841 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc cgctcttctg
     3901 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
     3961 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa
     4021 aatgcgtgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
     4081 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     4141 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     4201 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     4261 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa
     4321 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     4381 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     4441 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
     4501 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     4561 tttattgccc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
     4621 cgggcgtttt ttcagattgg gcatttcttt tctttttcgc cactgcacga ttgtttgact
     4681 gatgatttcc tgtgtcggca aggtcaaatc cgtagccgtg cataaacgcg tttcaggatg
     4741 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
     4801 ctgcgtctcg ccgcactggc gcgaacgctg ttccaaagcc cgaaaagcct gaatccgctc
     4861 gtttttttca gacggcagat agcctttaaa cgcaaaactc tgcccgttcg cacccgaagc
     4921 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
     4981 atgcgccaat gccaccaaat tcgcacccgg atcggccaca gccgggcaac ccgcctcact
     5041 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
     5101 atccgtatgt tcattcaacg tttgcagatt cagctcgcgg ataggcgtag tcacgcccaa
     5161 atgtttcaaa tgcgcacgcg ccgttttttc cgcctccacg acaaaatccg tcagcccgac
     5221 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     5281 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
     5341 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
     5401 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
     5461 caatcagcaa tgcacccaaa tcctcactct tcagcgcaca cgaacaataa accgcatcag
     5521 gctcgcgctc caaatagcgg aggatatgca actcgtccaa ctgcctcatc acgaccaccg
     5581 tcttatcgat atgccgccgc atcctgcccg taaccgtatt caacagcacg atcgcgctgt
     5641 aaaactcaat ctccctgccg ctcaagtgca tcagcatctt ttgcgcattg gcaaggttca
     5701 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     5761 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     5821 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     5881 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     5941 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcggtaaa
     6001 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     6061 aaatattgac gttagaacat ctaaattata tcatatctcg tttatgtcag accctaattt
     6121 gattgacccg gaaattttcg ccgccgaagg gcagaatctg caaggcagtt ttctgctgga
     6181 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     6241 gtttacactg accggcggtc gcgatcggct gcaacgcctg ttcctcgacc tgaacgtcaa
     6301 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     6361 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     6421 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     6481 ccaaatcctg atgtcgctcc ccttctctcc ccggcacgaa gactgcggca gcaacgggac
     6541 accggaaaaa gccgatcggg acaaaccaaa ccccttcgcc gttttggcag gtttgaaaag
     6601 caattgatta ggacacagtt tatttatcca ggagcttgaa atggccgttc aacaaaacaa
     6661 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcccgcact
     6721 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     6781 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     6841 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     6901 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     6961 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     7021 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
     7081 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
     7141 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
     7201 acaggttacc gaagaagtca aaaaagtcca atggctcgac cccgttcccg aagccctgcg
     7261 gggcaaaaac gtactcgtcg tcgatgaagt ggacgacagc cgcgtaacca tggagttctg
     7321 cctgaaagaa ctgctcaagg aagacttcgg tacgatcgga gtcgccgtac tgcacgaaaa
     7381 aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg gcatcaccgt
     7441 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
     7501 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
     7561 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaaaccct atgattacat
     7621 tggccgtaga tgccatgggc ggcgaccaag gacttgccgt taccgtaccc ggcgcaaccg
     7681 cattcctcca agcacacccc gatgtccgcc tgattatgac cggcgacgaa acgcaactgc
     7741 gccaagccct gaccgcggca ggcgcaccga tggaacgcat cgacatctgc cataccaccc
     7801 aagtcgtcgg catggacgaa gccccgcaat ccgccctgaa aaacaaaaaa gactcctcca
     7861 tgcgcgtcgc catcaaccag gttaaagaag gcaaagccca agccgccgta tccgcaggca
     7921 acacgggtgc gctcatggca accgcacgtt tcgtcctcaa aaccattccc ggcatcgaac
     7981 gccccgccat cgccaaattc cttccttccg acaccgacca cgttaccctt gcactcgacc
     8041 ttggcgcgaa cgtcgactgc acgcccgaac agctcgccca atttgccgtt atcggcagcg
     8101 aactcgtcca cgcactccat cctcaaaaag gacagccgcg cgtcgggctg gtcaacgtcg
     8161 gcacggaaga catcaaaggt acggacaccg tcaaacaaac ctacaaactg ctgcaaaaca
     8221 gcaaactcaa ctttatcggc aacatcgaaa gcaacggcat cctgtacggc gaagcagatg
     8281 tcgtcgtcgc cgacggcttt gtcggcaacg tcatgctcaa aaccatcgaa ggcgcggtca
     8341 aattcatgag cggagccatc cgccgcgaat tccaaagcaa cctgttcaac aaacttgccg
     8401 ccgttgccgt cctacccgcc ctcaaagggc tgaaaaacaa actcgatccg cgcaaattca
     8461 acggggccat cctgctcggg ctgcgcggca tcgtcatcaa aagccacggc ggcacagacg
     8521 aaaccggttt ccgctacgca ctcgaagaag cctaccacga agccaagtcc gccggccttt
     8581 ccaaaatcga acaaggcgtt gccgaacaac tcgccgcact cgaaactgcc aaagccgtcc
     8641 aaaacgaaaa tgccgacggt ctgtaacaca cacgatgccg tctgaacgcc cccgcccctt
     8701 tcagacggca tccgcccgca ccaaacctgc gggcgcggac ggcgatgcgc ctgtccggca
     8761 cttcccaaat acttccctgt aaaataagga gtatttgaaa aatgaagaca ttagaaaaac
     8821 ggatgaaagc tctagacaaa cagatgatga agttcggaaa atcccttgaa ggcaggcttg
     8881 atgcccgtct gattgaatcc gcattggatt atattcatta ttcggaacgt tttttggctt
     8941 ttgaaatcct gtgtacttat atcgaagatt tcgatgtccg gctgacggaa caagaatccc
     9001 gggaaatttc ttttatcaac aaggaatttg agatagaaag cacgtccgat taaccaataa
     9061 agccaatggg ttgataaaca tgaaaacatc gacggtcgtt tttggcggat tttttatggc
     9121 agacaacgga gagcgaatcc aaatccccgt tttggaaaat cctgacatta gggaaatcaa
     9181 tcactttttt tccgtatcaa attttgagaa aaaaaccggc gtccttgttt tcagaatcat
     9241 ccccgagccg gaatttggca ataccgaatt aactgtctat tttgaaaaag gatattattt
     9301 gccaatgatt caaacaattt tagaaggcgg ggatattgaa gtcaaaaacc ttaaaacgga
     9361 aaattatagt ggaaatacaa tggaaatttg gggagattcc taccctattg agcatatctc
     9421 taaaaatatt tctacaattc aaaatataat ttctgaattt atgatacaaa aacaaacacc
     9481 ggcaccaata atttgatgga aaaccaacaa tcaaaatgcc gtctgaagca tgtttggttt
     9541 tcagacggca tttccttcgc ttaaaacagc gtatcggcaa ccccgccctg cctgtccacg
     9601 gcaatctgca tctgaaaacc atctgtatcc caaaccacac ccccatccct gtttccgtca
     9661 tttgcaccct gcccgtattg ggcaatcatc ggtttttcgc ttacaatagc cgaatctgaa
     9721 ccaactatcc aaaaaggccg ttcccatgca gtatgccaaa atttccggca cgggcagcta
     9781 tcttcccgcc aaccgcgtca gcaatgacga ccttgcccaa aaagtggata cctcggacga
     9841 gtggattacc gcgcgtacgg gcatcaagtt ccgccatatt gcagccgaaa atgagaaaac
     9901 cagcgatctt gccgccgaag cagcccgccg cgcactggat gcagccggat tagacagcgg
     9961 cgaaatcgat ttgattatcg tggcgacggc aacgccggat atgcagtttc cttctaccgc
    10021 caccatcgtg cagcaaaaat tgggcatcac caacggctgt cccgcgtttg acgtacaggc
    10081 agtgtgcgca ggctttatgt acgcgctgac cactgcaaac gcctacatca aaagcggcat
    10141 ggcaaaaaac gcgctggtca tcggcgcgga aaccttcagt cgcattgtag actggaacga
    10201 ccgcacaacc tgcgtattgt tcggcgacgg cgcgggcgcg gtggttttga gcgcggcgga
    10261 caaaccgggc atcatccaca gcaaactcaa agccgacggc aattatctga aactcttaaa
    10321 cgtccccggg caaatcgcct gcggcaaagt ttccggttcg ccgtacattt cgatggacgg
    10381 tcccggcgtg ttcaagtttg ccgtcaaaat gctgtccaaa atcgccgatg acgttatcga
    10441 agaagccggt tacaccgccg ctcaaatcga ctggatcgtg ccgcatcagg caaaccgccg
    10501 cattatcgaa tcgaccgcga aacatttggg gctgagtatg gacaaagtcg tcctgaccgt
    10561 ccaagaccac ggcaacacgt ccgccgcctc gattccgctg gctttggata caggcatccg
    10621 cagcggacaa atcaaacgcg gtcaaaacct gctgctcgaa ggcatcggcg gtggtttcgc
    10681 gtggggcgcg gtgctgttgc aatattgaac ccgatgccgt ctgaagcagg ttttcagacg
    10741 gcatttccca tatcatgaag cggcaggctt tcttcaaacc aattgcgtgt gcggcatttc
    10801 tgtctgccgt ttcgctgcgc ctccccgtat tgggcgcgtg ttacgcaata ttgtccctct
    10861 atgcgtttgc actttacagc atcgacaaac ggcgtgccgt gcggggaaaa cgccgcattc
    10921 ccgaacaccg cctgctgctg cctgccttgt tcggcggttg ggcgggcgca tacttgggca
    10981 gcaggatatt caggcataaa acggcgaaaa agcgttttgt tgtgctgttc cgtctgactg
    11041 tttcgggcaa tgtcttggcg acctgcatcc tgattgatta tttcgttccg cccgaacttt
    11101 ttgtcaaact cgggcaacat ctctgacaat ccaaccaaac aaaggaatac catgtctttt
    11161 gcattcttct ttcccggaca aggttctcaa agcctcggca tgatgaacgg ctttacaggg
    11221 caggccgtcg tcaaaaacac atttgacgaa gcctccgcca tattggggca ggacttgtgg
    11281 gcgatgatta acggcagcga tgccgaaatc atcggtcaaa ccgtcaatac ccaacccatc
    11341 atgctcgccg ccggcgttgc cgtttaccgc gcctatttgg aagcgggtgg caaaacgcct
    11401 gccgccgttg ccggacacag cctcggcgaa tataccgcgc ttgtcgccgc cggcgcattg
    11461 gattttgcca acgcggtcaa actcgtgcgc ctacgcgcag aactgatgca gtccgccgta
    11521 ccgcaaggcg tgggcgcaat ggcggcaatt ttgggtttgg aagatgaaca ggtaaggcag
    11581 atttgcaccg aatccgcaca ggatgaagtg gttgaagccg tcaacttcaa ctcgcccgga
    11641 caagtcgtaa ttgcaggcaa tgccgccgcc gtcggacgcg ccatgaccgc cgccaaagaa
    11701 gccggtgcca aacgcgccct gccgctgccc gtgtccgtac cttcccattg cagcctcatg
    11761 aaacccgccg ccgacaaact cgccgaagcc ctcaaaaccg tcgaaatcaa gcagccgcaa
    11821 atccgcgtca tccacaacgc cgacgttgcc gcctacgatg atgccgacaa aatcaaagac
    11881 gcgctcgtcc gccagcttta cagccccgta cgctggacgg aaaccgtcaa cgcccttgtt
    11941 tcagacggca ttgccgaatc cgccgaatgc ggaccgggca aagtgttggc gggcttggca
    12001 aaacgcatca acaaaaccgc cgcgtgcagc gcgctgaccg atgccggaca ggttgccgcc
    12061 tttatcgaag cgcactgact tcgttccgca aaaagcagcc tgccctcttc aggctgcttt
    12121 tcacgtccga acggcaaaca gccgcatatt tacgctataa tccatcccga ccaaaccacc
    12181 gacagcggct gccgttgcag tttccgcccc accgatatga tagaaaaact gactttcgga
    12241 ctgtttaaaa aagaagacgc gcgcagcttt atgcgcctga tggcatacgt ccgcccctac
    12301 aaaatccgca tcattgccgc cctgattgcc attttcggcg ttgccgccac cgaaagctac
    12361 cttgccgcct tcatcgcccc cctgattaac cacggctttt ccgcacctgc cgcgccgccc
    12421 gagctgtctg ccgccgccgg catcatttcc accctgcaaa actggcgcga acagtttacc
    12481 tatatggttt gggggacgga aaacaaaatc tggaccgtcc cgctcttcct catcatcctc
    12541 gtcgtcatcc gtggcatctg ccgctttacc agcacctatc tgatgacttg ggtctccgtg
    12601 atgaccatca gcaaaatccg caaagatatg tttgccaaaa tgctgaccct ttcctcccgc
    12661 taccatcagg aaacgccgtc cggcaccgta ctgatgaata tgctcaacct gaccgaacag
    12721 tcggtcagca acgccagcga catcttcacc gtcctcacgc gcgacacgat gatcgttacc
    12781 ggcctgacca tcgtcctgct ttacctcaac tggcagctca gcctcatcgt cgtcctgatg
    12841 ttccccctgc tctccctgct ctcgcgctac taccgcgacc gtctgaaaca cgtcatttcc
    12901 gactcgcaaa aaagcatagg cacgatgaac aacgtgattg ccgaaaccca tcagggacac
    12961 cgcgtcgtca agctgttcaa cgggcaggcg caggcggcaa accggttcga cgcgatcaac
    13021 cgcaccatcg tccgcctcag caaaaaaatc acgcaggcaa cggcggcaca ttccccgttc
    13081 agcgaactaa tcgcctcgat cgccctcgcc gtcgtcatct tcatcgccct gtggcaaagc
    13141 caaaacggct acaccaccat cggcgaattt atggcattca tcgtcgcgat gctgcaaatg
    13201 tacgccccca tcaaaagcct tgccaacatt agcatcccta tgcagacgat gttcctcgcc
    13261 gccgacggcg tatgtgcatt tctcgacacc ccgcccgaac aggacaaagg cacgctcgca
    13321 ccgcagcgtg tcgaagggcg catcagcttc cgcaacgtcg atgtcgaata ccgttcggac
    13381 ggcatcaaag ccctcgacgg cttcaacctc gacatcagac aaggcgaacg cgtcgccctg
    13441 gtcggacgtt ccggcagcgg caaatccacc gtcgtcaacc tgctgccccg ctttgtcgaa
    13501 ccgtctgccg gcaacatctg catagacggt atcgacatcg ccgacatcaa actcgactgc
    13561 ctgcgcgccc aatttgccct cgtctcccaa gacgtattcc tgtttgacga caccctgttt
    13621 gaaaacgtcc gatatagccg tcccgacgcg ggcgaagccg aagtcctatc cgccctccaa
    13681 gccgctaacc tgcaaagcct gatcgacgca tcaccgctcg gactgcacca gcccatcgga
    13741 tcgaacggca gcaacttatc cggcggacag cggcaacgcg ttgccattgc ccgcgccatt
    13801 ttgaaagacg cgccgatatt attattggac gaagccacca gcgcattgga caacgaatcc
    13861 gaacgcctcg tccaacaggc gctcgaacgc ctgatggaaa accgcaccgg catcatcgtc
    13921 gcccaccgcc tgaccaccat cgaaggggcc gaccgcatca tcgtaatgga cgacggcaaa
    13981 atcatcgaac aaggcacaca cgaacaactg atgtcccaaa acggctacta cacgatgtta
    14041 cgcaatatct caaacaaaga tgccgccgtc cggacggcat aaacaaaatg ccgtctgaaa
    14101 tggtacaatc gccccgaccc tttcagacgg catcatatcc gccgacccat ccgattatct
    14161 tcaatcaccg taaaacccat tatgacccaa gacaaaatcc tcatccttga cttcggttcg
    14221 caagttaccc agctcatcgc ccgccgcgtg cgcgaagccc acgtttactg cgagctgcat
    14281 tctttcgata tgcctttgga cgaaatcaaa gccttcaacc ccaaaggcat catcctctcc
    14341 ggcggcccca attccgttta cgaatccgac tatcaagccg ataccggtat ttttgatttg
    14401 ggcattccgg ttttgggcat ctgttacggc atgcagttta tggcgcacca cttgggcggc
    14461 gaagtgcagc ccggcaacca gcgcgaattc ggttatgcgc aagttaaaac catagacagc
    14521 gagctgacac gcggcattca agatggtgag ccaaacacac tcgacgtatg gatgagccac
    14581 ggcgacaaag tgtccaaact gcccgacggt ttcgccgtca tcggcaacac cccgtcctgc
    14641 ccgattgcca tgatggaaaa cgccgaaaaa caattctacg gcatccagtt ccaccccgaa
    14701 gttacccaca ccaaacaagg ccgcgccctg ttgaaccgct ttgtcttgga tatttgcggc
    14761 gcacaaccgg gctggacgat gccgaactac atcgaagaag ccgttgccaa aatccgcgaa
    14821 caggtcggca gcgacgaagt gattttaggt ctgtccggcg gcgtggactc ttccgtagcc
    14881 gccgcgctga ttcaccgcgc catcggcgac caactgacct gcgtgttcgt cgatcacggt
    14941 ttgttgcgcc tgaacgaaag caaaatggtg atggatatgt tcgcccgcaa cttgggtgtg
    15001 aaagtgatac acgtcgatgc cgaagggcag tttatggcga aactcgccgg cgtaaccgac
    15061 cccgagaaaa aacgcaaaat catcggtgcg gaatttatcg aagtatttga tgccgaagaa
    15121 aaaaaactta ccaacgccaa atggttggca caaggcacga tttaccctga cgtaatcgaa
    15181 tccgcaggtg caaaaaccaa aaaagcccac gccatcaaat cgcaccacaa cgtcggcggc
    15241 ctgcctgaaa acatgaagct caaattgctt gagcctttgc gcgatttgtt caaagacgaa
    15301 gtacgcgaat tgggtgtggc tttgggcctg ccgcgcgaaa tggtgtaccg tcatccgttc
    15361 ccgggtccgg gtttgggcgt gcgtattttg ggcgaagtga aaaaagaata tgccgacctg
    15421 cttcgtcagg cagacgatat tttcattcaa gaattgcgca atactaccga tgaaaacggt
    15481 acatcttggt acgacctgac cagccaggca ttcgccgtgt tcctgcccgt caaatctgtc
    15541 ggcgtaatgg gcgacggccg cacatacgat tacgtcattg ccttgcgtgc cgtgattacc
    15601 agcgacttta tgaccgcgca ttgggcggaa ctgccgtatt ccttgttggg caaagtgtcc
    15661 aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg tttatgatgt gagcggcaaa
    15721 ccgcctgcca ccatcgagtg ggaataaaca gcaaacatgg ctgccccgtc cggcgcagtc
    15781 cttcgattat cggaaaaaag gaaaaaatat gagcacacaa gatttaaacg gcaaaatcgc
    15841 tttggtaaca ggcgcatcgc gcggtatcgg tgcagcaatt gccgacacgc tggcggcagc
    15901 cggtgccaaa gtcatcggta cggcgaccag tgagagcggt gcggcggcga ttagcgagcg
    15961 gttggcgcaa tggggcggcg aaggccgcgt attaaattcc gccgaacctg aaaccatcga
    16021 aagcctgatt gccgacatcg aaaaagcgtt cggcaaactc gatattctgg tcaacaacgc
    16081 cggcatcacc cgcgacaacc tcctgatgcg catgaaagaa gaagagtggg acgacatcat
    16141 gcaggtcaac ctcaaatccg tgttccgcgc ttctaaagcc gttttgcgcg gtatgatgaa
    16201 acaacgttcc ggccgcatca tcaacatcac atccgtcgtc ggcgtgatgg gcaatgccgg
    16261 tcaaaccaac tatgccgcgg caaaagcagg cttaatcggt ttctccaaat ccatggcgcg
    16321 cgaagtcggc agccggggca ttaccgtcaa ctgcgtcgcc cctggcttta tcgataccga
    16381 catgacacgc gccctgccgg aagaaacccg ccaaaccttt accgcccaaa ccgccttggg
    16441 cagattcggc gacgcgcaag acatcgccga tgcggttctg ttcctcgctt ccgaccaagc
    16501 aaaatacatc accggccaaa cgctgcacgt caacggcggt atgctgatgc cttaacagac
    16561 aactttttca accatgccgt ctgaagccct ttcagacggc atttgcattc tcaggcaaaa
    16621 tgaacacaca ccacaccccg ccctgcccat gcggctcagg cacaagctga gacctttgca
    16681 aaattccttt ccctcccgac agccgaaacc ccaacacagg ttttcagctg ttttcagctg
    16741 ttttcgcccc aaataccgcc taattctacc caaatacccc cttaatcctc cccggacacc
    16801 tgataatcag gcatccgggt caccttttag gcggcagcgg gcgcacttag cctgttggcg
    16861 gctttcaa
//
LOCUS       DAWISC010000027        16814 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWISC010000027 DAWISC010000000
VERSION     DAWISC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.027"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            336..980
                     /gene="rplC"
                     /locus_tag="WIW87_000608"
     CDS             336..980
                     /gene="rplC"
                     /locus_tag="WIW87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ5173806.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            980..1600
                     /gene="rplD"
                     /locus_tag="WIW87_000609"
     CDS             980..1600
                     /gene="rplD"
                     /locus_tag="WIW87_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ5173807.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1597..1911
                     /gene="rplW"
                     /locus_tag="WIW87_000610"
     CDS             1597..1911
                     /gene="rplW"
                     /locus_tag="WIW87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ5173808.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVADVTTVTIKGKVKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1917..2750
                     /gene="rplB"
                     /locus_tag="WIW87_000611"
     CDS             1917..2750
                     /gene="rplB"
                     /locus_tag="WIW87_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ5173809.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLYKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2757..3035
                     /gene="rpsS"
                     /locus_tag="WIW87_000612"
     CDS             2757..3035
                     /gene="rpsS"
                     /locus_tag="WIW87_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ5173810.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3044..3373
                     /gene="rplV"
                     /locus_tag="WIW87_000613"
     CDS             3044..3373
                     /gene="rplV"
                     /locus_tag="WIW87_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ5173811.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3383..4075
                     /gene="rpsC"
                     /locus_tag="WIW87_000614"
     CDS             3383..4075
                     /gene="rpsC"
                     /locus_tag="WIW87_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ5173812.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4059..4475
                     /gene="rplP"
                     /locus_tag="WIW87_000615"
     CDS             4059..4475
                     /gene="rplP"
                     /locus_tag="WIW87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ5173813.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4475..4666
                     /gene="rpmC"
                     /locus_tag="WIW87_000616"
     CDS             4475..4666
                     /gene="rpmC"
                     /locus_tag="WIW87_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ5173814.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4666..4929
                     /gene="rpsQ"
                     /locus_tag="WIW87_000617"
     CDS             4666..4929
                     /gene="rpsQ"
                     /locus_tag="WIW87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ5173815.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5151..5519
                     /gene="rplN"
                     /locus_tag="WIW87_000618"
     CDS             5151..5519
                     /gene="rplN"
                     /locus_tag="WIW87_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ5173816.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5531..5854
                     /gene="rplX"
                     /locus_tag="WIW87_000619"
     CDS             5531..5854
                     /gene="rplX"
                     /locus_tag="WIW87_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ5173817.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5864..6403
                     /gene="rplE"
                     /locus_tag="WIW87_000620"
     CDS             5864..6403
                     /gene="rplE"
                     /locus_tag="WIW87_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ5173818.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6406..6711
                     /gene="rpsN"
                     /locus_tag="WIW87_000621"
     CDS             6406..6711
                     /gene="rpsN"
                     /locus_tag="WIW87_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ5173819.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6727..7119
                     /gene="rpsH"
                     /locus_tag="WIW87_000622"
     CDS             6727..7119
                     /gene="rpsH"
                     /locus_tag="WIW87_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ5173820.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7133..7666
                     /gene="rplF"
                     /locus_tag="WIW87_000623"
     CDS             7133..7666
                     /gene="rplF"
                     /locus_tag="WIW87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ5173821.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7680..8033
                     /gene="rplR"
                     /locus_tag="WIW87_000624"
     CDS             7680..8033
                     /gene="rplR"
                     /locus_tag="WIW87_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ5173822.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8052..8570
                     /gene="rpsE"
                     /locus_tag="WIW87_000625"
     CDS             8052..8570
                     /gene="rpsE"
                     /locus_tag="WIW87_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ5173823.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8563..8748
                     /gene="rpmD"
                     /locus_tag="WIW87_000626"
     CDS             8563..8748
                     /gene="rpmD"
                     /locus_tag="WIW87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ5173824.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8750..9184
                     /gene="rplO"
                     /locus_tag="WIW87_000627"
     CDS             8750..9184
                     /gene="rplO"
                     /locus_tag="WIW87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ5173825.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9196..10506
                     /gene="secY"
                     /locus_tag="WIW87_000628"
     CDS             9196..10506
                     /gene="secY"
                     /locus_tag="WIW87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ5173826.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10511..10729
                     /gene="infA"
                     /locus_tag="WIW87_000629"
     CDS             10511..10729
                     /gene="infA"
                     /locus_tag="WIW87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ5173827.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10750..10863
                     /gene="rpmJ"
                     /locus_tag="WIW87_000630"
     CDS             10750..10863
                     /gene="rpmJ"
                     /locus_tag="WIW87_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ5173828.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10929..11291
                     /gene="rpsM"
                     /locus_tag="WIW87_000631"
     CDS             10929..11291
                     /gene="rpsM"
                     /locus_tag="WIW87_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ5173829.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11311..11706
                     /gene="rpsK"
                     /locus_tag="WIW87_000632"
     CDS             11311..11706
                     /gene="rpsK"
                     /locus_tag="WIW87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ5173830.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11726..12346
                     /gene="rpsD"
                     /locus_tag="WIW87_000633"
     CDS             11726..12346
                     /gene="rpsD"
                     /locus_tag="WIW87_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ5173831.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12372..13358
                     /gene="rpoA"
                     /locus_tag="WIW87_000634"
     CDS             12372..13358
                     /gene="rpoA"
                     /locus_tag="WIW87_000634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ5173832.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13382..13750
                     /gene="rplQ"
                     /locus_tag="WIW87_000635"
     CDS             13382..13750
                     /gene="rplQ"
                     /locus_tag="WIW87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ5173833.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13897..14610
                     /gene="minC"
                     /locus_tag="WIW87_000636"
     CDS             13897..14610
                     /gene="minC"
                     /locus_tag="WIW87_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ5173834.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14638..15453
                     /gene="minD"
                     /locus_tag="WIW87_000637"
     CDS             14638..15453
                     /gene="minD"
                     /locus_tag="WIW87_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ5173835.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15457..15720
                     /gene="minE"
                     /locus_tag="WIW87_000638"
     CDS             15457..15720
                     /gene="minE"
                     /locus_tag="WIW87_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ5173836.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15724..16644
                     /gene="oxyR"
                     /locus_tag="WIW87_000639"
     CDS             15724..16644
                     /gene="oxyR"
                     /locus_tag="WIW87_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ5173837.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFICD"
     gene            complement(16641..>16814)
                     /locus_tag="WIW87_000640"
     CDS             complement(16641..>16814)
                     /locus_tag="WIW87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173838.1"
                     /translation="WHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNILAYRVV
                     GSGKWRFENNACA"
BASE COUNT         4613 a         3149 c         4175 g         4877 t
ORIGIN      
        1 cacagcctca tcacccgcat cgatttcaac ctgttttgcc gctttgacga actgagcatc
       61 cccgattaca gtcatcaacc atattccggt ttgtcggaga aagatgcata cgctgtgatg
      121 accggatacc gacccgttaa aagagtccga ccctatgccg tctgaaaatt caaaacgctt
      181 cagacggcat attgaagata tttctgatat ttctgttgat atttctttga cttgtcagat
      241 ataatgccga gcttggtaca tttgtgccaa gtttaacttt gtctgaaaga caggccaatc
      301 gtagcctgtc cctttacttt aaaaggaaaa taatcatgac tttaggtctg gttggacgca
      361 aagttggtat gacccgcgtg ttcgacgaac agggtgtttc tgttccggta accgttttgg
      421 atatgtctgc caaccgcgtt acacaagtaa aatccaaaga tactgacggc tatactgccg
      481 ttcaagttac ctttggtcag aaaaaagcca atcgtgtcaa caaagccgaa gccgggcact
      541 ttgcaaaagc aggtgttgaa gccggtcgcg gtttgattga gtttgctttg actgaagaaa
      601 aactggctga attgaaagct ggtgacgaaa tcaccgtttc tatgtttgaa gtcggtcaac
      661 tggtcgatgt aaccggtacc tctaaaggta aaggtttctc cggcacgatt aaacgtcata
      721 acttcggtgc ccaacgtact tcccacggta actcccgttc tcaccgtgtt ccaggctcta
      781 tcggtatggc gcaagacccg ggtcgcgtgt tccccggtaa acgcatggcc ggccaatacg
      841 gcaacaccaa agcaactgtt caaaaattgg aagttgtccg tgttgacgca gaacgccaac
      901 tgctgttggt taagggtgct gttccgggtg cggtcaacag cgatgttgta gttcgtccca
      961 gcgtgaaagt aggtgcgtaa tggaattgaa agtaattgac gctaaaggac aagtttcagg
     1021 cagtctgtct gtttctgatg ctttgttcgc ccgcgaatac aatgaagcgt tggttcatca
     1081 gctggtaaat gcctacttgg caaacgcccg ctccggtaac cgcgctcaaa aaacccgtgc
     1141 cgaagtaaaa cactcaacca aaaaaccatg gcgtcaaaaa ggtaccggcc gtgcccgttc
     1201 cggtatgact tcttctccgc tgtggcgtaa aggtggtcgc gcgttcccga acaaacccga
     1261 cgaaaacttc actcaaaaag taaaccgcaa aatgtaccgt gccggtatgg cgactattct
     1321 gtcccaattg actcgtgacg agcgtttgtt tgcgattgag gcgttgactg ccgaaactcc
     1381 taaaaccaaa gtttttgccg aacaagtgaa aaatctgggt ctggagcaag tgttgtttgt
     1441 aaccaaacag ctcgacgaga atgtttactt ggcttcacgc aacttgccaa acgtgttggt
     1501 tttggaagct caacaagttg atccttacag cttgctgcgt tacaaaaaag taatcatcac
     1561 taaagatgca gttgcacaat tagaggagca atgggtatga atcaacaacg tttgactcaa
     1621 gtgattttgg cacctatcgt ttctgaaaaa agcaacgtat tggctgaaaa acgtaaccaa
     1681 atgacgttta aagttttggc aaatgcaacc aaacctgaaa ttaaagcggc tgttgagctg
     1741 ctgttcggcg ttcaagttgc agacgttact actgttacca ttaaaggtaa agttaaacgt
     1801 tttggtcgca ctttaggtcg tcgcagcgat gttaaaaagg cttatgtaag cttggctgcc
     1861 ggtcaagagt tggatttgga agccgctgct gcagctgcag ataaggaata aacaaaatgg
     1921 caatcgttaa aatgaagccg acctctgcag gccgtcgcgg catggttcgc gtggtaacag
     1981 aaggtttgta caaaggtgca ccttatgcac ctctgctgga aaagaaaaat tctactgccg
     2041 gtcgtaacaa caatggtcat attactaccc gtcataaagg tggtggtcat aaacatcatt
     2101 accgcgtcgt agattttaaa cgtaacaaag acggtatccc tgcaaaagta gagcgtatcg
     2161 aatatgaccc taaccgtact gcatttatcg cactgttgtg ctatgcagat ggtgagcgtc
     2221 gctacattat tgctcctcgt ggtattcaag ccggtgcagt attggtttcc ggtgctgaag
     2281 ctgcgatcaa agtaggtaac actctgccga tccgcaatat tcctgttggt acaactattc
     2341 actgtatcga aatgaaacca ggtaaaggtg cgcaaattgc acgttctgcc ggtgcttctg
     2401 cggtattgct ggctaaagaa ggcgcgtacg ctcaagtccg cctgcgctct ggcgaagtcc
     2461 gtaaaatcaa cgtagattgc cgtgcaacca tcggtgaagt cggtaacgaa gagcaaagcc
     2521 tgaaaaaaat cggtaaagcc ggtgccaatc gttggcgcgg tattcgtccg actgtacgtg
     2581 gtgttgtcat gaaccctgtc gatcacccgc atggtggtgg tgaaggccgt acgggcgagg
     2641 cccgcgaacc ggtcagccca tggggtactc ctgctaaagg ctaccgcact cgtaataaca
     2701 aacgcacgga taacatgatt gttcgtcgcc gttactcaaa taaaggttaa tttagtatgg
     2761 ctcgttcatt gaaaaaaggc ccatatgtag acctgcattt gctgaaaaaa gtagatgctg
     2821 ctcgcgcaag caacgacaaa cgcccgatta aaacctggtc tcgtcgttct accattctgc
     2881 ctgattttat cggtctgacc attgctgtgc acaacggccg cacccatgtg cctgtgttta
     2941 tcagcgacaa tatggttggt cataaattag gcgaattctc attgacccgt acctttaaag
     3001 gccacttggc cgataaaaag gctaaaaaga aataaggtga atcatgagag taaatgcaca
     3061 acataaaaat gcccgtatct ctgctcaaaa ggctcgtttg gtagctgatt tgattcgtgg
     3121 taaagacgtt gcccaagctt tgaatatttt ggctttcagt cctaaaaaag gtgccgagct
     3181 gattaaaaaa gtattggagt cagctattgc taatgccgag cacaataacg gtgcggacat
     3241 tgatgaactg aaagtggtaa ctatctttgt tgacaaaggc ccaagcttga aacgttttca
     3301 agctcgcgcc aaaggtcgcg gtaaccgcat cgaaaaacaa acttgtcata tcaatgtgac
     3361 agtgggtaac taaggaaaag ctatgggaca aaagattaac cctacaggct ttcgcctggc
     3421 ggtaactaaa gactgggctt caaaatggtt tgctaaaagc accgactttt ctactgtttt
     3481 gaagcaggat atcgatgttc gcaattattt gcgtcaaaaa ttggccaatg cttcggttgg
     3541 tcgagtggtt attgaacgcc ctgcaaaatc tgcacgcatt accattcact ccgctcgtcc
     3601 gggtgtggtt atcggtaaaa aaggtgagga tatcgaggtt ttgaaacgtg acttgcaagt
     3661 cttgatgggt gtacctgttc atgtaaatat tgaagagatt cgccgtcctg agttggatgc
     3721 tcaaattatt gctgacggta ttgcccagca gttggaaaag cgcgttcaat tccgtcgtgc
     3781 tatgaaacga gcaatgcaaa atgcaatgcg ttctggtgct aaaggcatta agattatgac
     3841 ttcaggccgt ctgaatggtg cggatattgc ccgtagcgaa tggtatcgtg aaggtcgcgt
     3901 gccactgcat actttacgtg caaatgtaga ttatgcaacc agcgaagcgc acaccacata
     3961 tggtgtattg ggtctgaaag tttgggttta tacggaaggc aatattaaat cttccaaacc
     4021 tgaacatgag agtaaacaaa gaaaggcagg tagacgtaat gctgcagcca actagactga
     4081 aataccgtaa gcaacaaaag ggtcgcaata ccggcatcgc tactcgcggt aataaggtaa
     4141 gtttcggtga gttcggcttg aaagccgtag gtcgtggtcg tttgactgcc cgtcaaatcg
     4201 aagctgctcg tcgtgcaatg acccgtcata tcaaacgtgg tggtcgtatt tggattcgtg
     4261 tattccctga taaaccgatt actgaaaagc ctattcaagt tcgtatgggt ggcggtaaag
     4321 gtaacgtgga atattacatt gccgaaatta aaccaggtaa agtgttgtat gaaatggatg
     4381 gcgttccaga ggaactggct cgtgaagcat tcgagttggc tgctgccaaa ttgcctattc
     4441 ctacaacctt tgtagtaaga caggtgggtc aataatgaaa gcaaatgaat tgaaagacaa
     4501 atccgttgag cagttgaatg cagatttgtt ggacttgttg aaagctcagt ttggcttacg
     4561 tatgcaaaac gctaccggtc aattaggcaa accaagtgaa ttgaaacgtg tacgtcgcga
     4621 tattgctcgt attaaaaccg ttttaactga aaaaggtgct aagtaatgag cgaaactaaa
     4681 aatgttcgta ctttgcaagg caaagtagta agcgacaaaa tggataaaac cgtaacagta
     4741 ttggttgagc gtaaagtaaa acatccgctg tatggtaaga ttattcgatt atctactaaa
     4801 atccatgccc atgatgaaaa taatcaatat ggaattggtg atgtggttgt tatatcggaa
     4861 tcccgtccat tgtcaaaaac taaatcttgg gttgtcagtg agctggttga gaaagcacgt
     4921 tctatttaag aattaaagca acgtgcttgg aatgggaaac gaagtattgc agcaaattta
     4981 atttgcgtgt aaacttcgtt tcctgtcttt cagtttcttc tggaagtttc ttccctttcg
     5041 gggtccaaga ctggtttact tgaaccgcaa ggtttcattt aataagcagc ggctttgctg
     5101 taagttatct gaaagtggta aattaagttg gttaatttaa aggtaataac atgattcaaa
     5161 tgcagaccat cttagatgtg gctgataact ctggtgcgcg tcgcgtaatg tgtatcaagg
     5221 tattgggcgg atctaagcgt cgctacgctt ctgttggcga tattattaaa gtggcagtta
     5281 aagatgcggc tccgcgtggc cgtgtcaaaa aaggcgatgt atataatgcg gtagttgttc
     5341 gtactgctaa gggtgtacgt cgtcctgatg gtgcgttaat taaattcgat aacaatgccg
     5401 ccgtgttact gaataataaa cttgaacctt tgggtactcg tatctttggt ccggtaaccc
     5461 gtgaattgcg tactgagcga tttatgaaaa tcgtttcatt ggcacctgaa gtattataag
     5521 gaatggcacg atgaataaaa tcattaaagg cgatagggtt gtagtaattg ctggtaagga
     5581 taaaggtaag cagggtcaag tagttcgagt gttgggtgat aaagttgttg ttgagggcgt
     5641 taatgttgta aaacgccatc aaaaacctaa tccaatgcgt ggcattgagg gcggtattat
     5701 tactaaagaa atgcctttgg atatttctaa tatcgcaatc ctgaatccgg aaactaataa
     5761 agcggaccgt gttggtatta agctgattga aaatgaaggc aaagttaaac gcgttcgttt
     5821 cttcaaatca aatggctcta tcattggggc ataaggagat aacatggctc ggttgagaga
     5881 gttttataaa gagacagttg ttcctgaatt ggttaaacaa tttggttaca aatcagtaat
     5941 ggaagtcccg cgtattgaaa aaattacctt gaatatgggt gtgggtgagg ctgttgctga
     6001 taaaaaagtt atggaacatg ctgtttccga tttagagaaa attgccggtc aaaaaccggt
     6061 tgttactgtt gcccgtaaat ctatcgcagg ttttaaaatc cgtgataact atccggttgg
     6121 ttgcaaagta acattgcgtc gtgatcaaat gtttgaattc ttggatcgtt tgattactat
     6181 tgcattacct cgcgtacgtg acttccgtgg tgtgagcggt aaatcatttg atggccgtgg
     6241 caattacaat atgggtgttc gtgagcaaat tatttttccg gaaattgaat acgataaaat
     6301 tgatgctttg cgtggtttga atattactat tactactaca gcaaaaaccg atgaggaagc
     6361 gaaagcttta ttgtcattgt ttaaatttcc gttcaaagga taatcatggc taagaaagca
     6421 cttattaatc gtgatctgaa acgtcaagct ttggctaaaa aatatgcggc taaacgcgcg
     6481 gcaattaaag cggtaatcaa tgattcgaat gcaactgagg aagagcgttt tgaggctcgt
     6541 ttgaggtttc aatccattcc tcgtaatgcg gcacctgtgc gtcaacgtcg tcgttgtgct
     6601 ttgacaggtc gccctcgtgg tactttccgt aaatttggtt tgggtcgtat taaaatccgt
     6661 gaaatcgcca tgcgtggcga aattccgggt gttgttaaag ccagctggta ataggagtaa
     6721 ttaagaatga gtatgcatga tcctatttcc gatatgttga ctcgtatccg caatgcgcaa
     6781 cgtgctaata aagcagcggt tgcaatgcct tcttcaaaat taaagtgtgc tattgcaaag
     6841 gtattgaaag aagaaggata tattgaggac ttcgcagttt catctgacgt aaagtctata
     6901 ttggaaattc aattaaaata ctatgcaggt cgtcctgtaa ttgaacaaat caagcgtgta
     6961 tctcgccccg gtttgcgtat ttataaagcg tctagtgaga ttccaagtgt tatgaatggc
     7021 ttgggtattg ctattgttag tacttctaaa ggtgtaatga ctgatcgtaa agcacgttct
     7081 caaggtgttg gtggtgagtt gttatgcatt gtagcctagt ggaggaaaag aaatgtcacg
     7141 tgtcgcaaaa aacccagtga ctgttcccgc tggtgtagaa gtaaaatttg gagcagaggc
     7201 attagttatt aagggtaaga acggtgaatt gtcttttcct ttgcattctg atgtagccat
     7261 tgaatttaat gatggcaaat tgacttttgt tgcgaataac agcagtaaac aagcaaatgc
     7321 aatgtctggt actgctcgcg cattagtcag caatatggtt aaaggtgttt cagaaggttt
     7381 tgagaaaaga ttgcaattga taggtgtggg ttatcgtgct caagcacaag gtaaaatctt
     7441 gaatctgtct ttgggttttt ctcatccgat cgtatatgaa atgcctgaag gtgtctccgt
     7501 tcaaactcct agccaaacag agattgtttt aaccggctcg gataaacaag ttgttggtca
     7561 agttgctgct gagattcgtg cgttccgtgc tcctgagcct tataaaggta aaggtgttcg
     7621 ctatgtagga gaagtagtgg taatgaaaga agccaagaaa aaataattga ggttcactaa
     7681 tggataaaca tacaacccga ctccgtcgtg cacgcaaaac ccgtgctcgt attgcggact
     7741 tgaaaatggt aagattatgt gtgttccgaa gcaataatca tatttatgct caagtaatta
     7801 gtgctgaagg tgataaagta ttggctcaag cctctacatt ggaagctgag gtgcgcggta
     7861 gtctgaaatc tggaagcaat gttgaagcag ctgcaatagt tggtaaacgt atcgctgaaa
     7921 aagctaaagc agcaggtgta gaaaaggttg cttttgatcg ttcaggtttc caatatcacg
     7981 gtcgtgtgaa ggctttggct gaagctgctc gtgaaaatgg tttaagcttc taaatatttg
     8041 gagactttca gatggcaaaa catgaaattg aagaacgcgg tgacggtctg attgaaaaga
     8101 tggtcgctgt taatcgcgta actaaagtag ttaaaggtgg ccgtatcatg gctttctcag
     8161 cactgactgt tgttggtgat ggtgatggtc gcattggtat gggcaaaggt aaatcaaaag
     8221 aagtaccagt tgctgttcaa aaagcaatgg atcaagctcg acgctctatg attaaagtac
     8281 ctttgaaaaa cggtactatt catcatgagg ttattggccg tcatggtgct actaaagtat
     8341 ttatgcagcc tgctaaagag ggtagtggcg taaaagccgg tggacctatg cgtttggttt
     8401 ttgatgctat gggcattcat aatatctccg ccaaagtgca cggatctact aacccatata
     8461 atatcgtacg tgcaacatta gatggtttgt ctaagttgca tactcctgct gatatcgcag
     8521 ccaaacgtgg cttgacagtg gaagacattt tgggagttaa ccatggctga acaaaaaaag
     8581 attagggtta cattggttaa aagcctgatt ggtacaattg aatctcatcg tgcatgtgca
     8641 cgcggtttag gtttgcgtcg tcgcgagcat acggtagagg ttttagatac ccctgaaaac
     8701 cgtggtatga ttaataaaat cagctacttg ttgaaagtgg agtcttgata tgtttttgaa
     8761 tacaattcaa cctgctgttg gtgctacgca tgctggtcgt cgtgttggac gcggtattgg
     8821 tagtggtctt ggcaaaacgg gtggtcgtgg tcataaaggt caaaagagcc ggtctggtgg
     8881 gtttcataag gtgggtttcg agggtggtca aatgcccttg caacgacgcc tccctaaaag
     8941 aggttttaaa tctttaacag catcagctaa tgcacagctt cgtttaagtg aactggaatc
     9001 aattgctgtt aatgagattg atattttggt cttaaagcaa gcgggtctga ttgcatctac
     9061 agtctctaat gttaaagtta ttgcttctgg tgaaatttct aaggcagttg ctttgaaggg
     9121 tattaaagtt accaaaggtg cgagagctgc tatcgaggct gttggtggta agattgaaat
     9181 gtaaggttta atattgtggc taatcaacaa acgtcatcag gttcatccaa atttggagat
     9241 cttaagaaac gtcttttgtt tctatttgga gcattgattg tttttcgaat tggtgcccat
     9301 atacccgtac ctggagttga tgctgttgct ttagctaaat tatacgaaag cgctggaaac
     9361 ggcatcctgg gaatattgaa tatgttttcc ggtgggtcgt tagagcgctt tagtatattt
     9421 gcaataggaa ttatgccata tatttcagct tctattattg tacagctcgc ttctgaaatt
     9481 ttgccatcat tgaaggcttt aaaaaaagaa ggggaggctg gtagaaaggt aattacgaaa
     9541 tatactaggt atggtactgt tttgttagca attcttcaaa gtctaggtgt tgcatctttc
     9601 gtatttcagc aaggaattgt tgtaacaagt tcatttgagt ttcatgtttc cacggtagtt
     9661 tctttggtaa cgggaaccat gtttcttatg tggcttgggg agcaaattac tgaaaggggt
     9721 atcgggaacg gtatttcttt aatcattacg gcaggtattg cttcaggtat tccttcgggt
     9781 attgcaaagc tggttacact gacgaaccaa ggttctatga gcatgcttac ggcgttgttt
     9841 attgtatttg gtgccttatt attaatttat ttggttgtat actttgaaag tgcacagcgg
     9901 aagattccta ttcattatgc aaaacgccag tttaatggta gggcgggtag tcaaaatacg
     9961 catatgcctt tcaagttgaa tatggctggt gttattcccc caatttttgc ttccagtatt
    10021 attctatttc catctactct tttaggttgg tttggttcgg ctgatacaaa tagtgttttg
    10081 cacaaaatag ctggattgtt acaacacggt caattgctgt atatggcttt atttgcagcg
    10141 acagttattt tcttttgtta tttttatacg gctttggttt ttagccctaa agaaatggca
    10201 gagaatttaa aaaagagtgg tgcttttgtt cctgggatta gacctggtga gcagacctct
    10261 aggtatttag aaaaagttgt attacgtttg acattgtttg gagctcttta tattacaact
    10321 atttgtttaa ttccagagtt cttaactacg gttttaaatg taccttttta tttgggtggc
    10381 acgtctttgt tgattctagt tgttgtaacg atggatttta gtacacaaat aaattcgtat
    10441 aggcttactc aacagtatga taagttaatg actcgttcag aaatgaaatc attttctcgg
    10501 aaatagaatt atggcgaaag aagatactat ccaaatgcaa ggtgaaattc ttgaaacttt
    10561 acctaatgca acatttaaag taaaacttga gaatgaccat attgtattgg gtcatatttc
    10621 tgggaagatg cggatgcatt acattcgtat ttctccggga gataaggtca cagtagagct
    10681 gacaccttat gatctaacta gggctcgaat cgttttcaga gcaagataaa ccaataaaag
    10741 gaaaataaaa tgcgtgtaca accatctgtt aagaaaattt gccgaaattg caagattatt
    10801 cgtcgaaatc gtgtagttcg tgtaatttgt actgatctcc gtcacaaaca gcgtcaaggt
    10861 taatggaata tttcttttaa tgtgattctg tgatatagtg acacactttg ccctaaaaag
    10921 gaaaaaatat ggctcgtatt gcaggggtaa atatccctaa taacgcacac atcgtaattg
    10981 gtcttcaggc tatttacggt attggtgcta ctcgtgctaa attgatttgt gaggctgcaa
    11041 atattgcgcc tgatactaaa gcaaaagatt tggacgagac tcaattagat gctttgcgtg
    11101 accaagttgc caagtatgaa gtagaaggtg atttgcgtcg tgaggtaact atgagtatca
    11161 agcgattgat ggacatgggc tgctatcgtg gcttccgtca tcgtcgcggc ttaccatgcc
    11221 gcggtcaacg cactcgtaca aatgcgcgta cccgcaaagg tccgcgtaaa gcgattgctg
    11281 gtaagaaata aattttaagg aattttatta atggctaaag caaacacagc ttcacgtgta
    11341 cgtaaaaaag tacgtaaaac cgtgagtgag ggtattgtgc acgttcatgc atctttcaac
    11401 aataccatca ttacaatcac tgaccgtcaa ggcaatgcgt tgtcttgggc tacctctggc
    11461 ggcgctggtt ttaaaggttc tcgtaaaagt acaccatttg cagcacaagt tgcagcagaa
    11521 gcagctggta aagttgccca agagtatggc gttaaaaatt tagaggttcg tattaaaggt
    11581 ccaggtccag gtcgtgaatc ctctgtacgt gctttgaatg ctcttggttt caagattacc
    11641 agcattactg acgttacccc gttgcctcat aacggttgcc gtccgcctaa aaaacgtcgt
    11701 atttaatatt ggagtgattt gaaacatggc acgttatatt ggccctaaat gtaagttggc
    11761 acgtcgcgaa ggtacggatt tgtttttgaa gagtgcgcgc cgctctttgg attctaaatg
    11821 taaaattgat tccgctcctg gtcagcatgg tgcaaaaaaa ccgcgtttgt cagactatgg
    11881 tttgcagttg cgtgaaaaac aaaaaatccg ccgtatttat ggcgtattag aacgtcagtt
    11941 ccgtcgttat ttcgcagaag ctgatcgtcg taaaggttct accggcgagt tgctgttgca
    12001 gttgctggaa tctcgtttgg ataatgtcgt ttatcgtatg ggtttcggtt ctacccgagc
    12061 tgaagcaaga cagcttgttt ctcataaggc gatagttgtg aatggacaag ttgtcaatat
    12121 tccttctttc caagtgaaag ctggtgatgt tgtctcagtt cgtgaaaaag ccaaaaaaca
    12181 ggtacgtatt caagaagcat tgggtttggc aactcaaatc ggcttgccgg gttgggtttc
    12241 tgtagatgcg gataaacttg agggtgtgtt caaaaacatg ccggatcgct cggaattgac
    12301 cggtgatatt aatgaacagc tggtggtaga gttctactct aaataatgct agctcagtga
    12361 gggacagtta aatgcagaat agcacaaccg aatttttgaa acctcgtcaa attgatgtaa
    12421 atactttttc tgcaactcgt gcaaaagtat ctatgcagcc atttgaacgt ggtttcggtc
    12481 ataccttagg taatgctttg cgccgtatct tactgtcatc catgaatggt tttgctccta
    12541 ctgaagtagc tattgccggt gtattacacg aatattctac tgttgatggt attcaggaag
    12601 atgttgttga cattttgctg aatattaaag gtattgtgtt taaactccat ggtcgtagcc
    12661 aagttcaact tgtgttgaag aaatcaggtt caggtgtcgt atctgccggt gatattgagt
    12721 tgccgcatga tgtagaaatt ctgaatcctg gtcatgtcat ttgtcatttg gctgataacg
    12781 gtcaaattga gatggaaatt aaagtagagc aaggtcgtgg ttatcaatct gtttcaggtc
    12841 gtcaggtagt tcgtgatgag aaccgtcaga ttggtgcaat ccagttggat gcgagctttt
    12901 cgcccatcag ccgtgttagc tttgaggttg aacctgcacg tgtagagcag cggacggatc
    12961 ttgataagtt ggttttggat atcgaaaccg acggttctat tgatcctgag gaagctgtac
    13021 gcagtgcggc acgtattttg attgatcaga tgtctatttt tgctgatttg cagggtacgc
    13081 ctgtggagga ggttgaagaa aaagcacctc ctatcgaccc tgttcttttg cgtccggtgg
    13141 atgatctgga attgacagta cgttcagcta attgtttgaa agctgaggat atttattata
    13201 ttggcgattt gattcaacgc actgaaaccg agcttcttaa aacgccgaat ttgggacgta
    13261 aatctttgaa tgagattaag gaagtattgg catctaaagg tttgacactg ggttctaagt
    13321 tggaagcatg gccacctgta ggcttggaaa agccttaatg aagaattaaa ggataattga
    13381 tatgcgtcat cgtaatggca atcgcaaatt aaaccgtacc agcagtcatc gtgctgcaat
    13441 gctgcgtaat atggcgaatt cattattgac tcacgaagct attgtaacaa ctctgcctaa
    13501 ggccaaggaa ttgcgccgtg tagtagagcc gttgattaca ttgggtaaaa agccgtcatt
    13561 ggcaaaccgc cgtttggcat ttgaccgtac tcgcgaccgt gatgttgtag taaaactgtt
    13621 tggcgatttg ggtcctcgtt ttactgctcg taacggtggt tatgttcggg tgttgaaata
    13681 cggattccgt aaaggtgata atgcacctct ggcactggtt gaattggttg acaaaccggc
    13741 tgctgagtaa ttttagtcat ataacgccat ctgccgaaaa gcaggtggcg ttatttttgc
    13801 aatatctgat aggtaatagg gtattggcta tcatgtttaa aatattaatt gaatagctaa
    13861 ggtttgcgcg gtaaacttac atcattaaaa aattctatga tggtttatat aatgaatgct
    13921 ttcgatataa agtcgacaaa gatggacgta ttgtctatat ctttgcatac gtcagacttg
    13981 tttgatttgg aagatgtgct ggtcaaattg ggcaagaagt ttcaagagtc tggtgttgtt
    14041 ccatttgtgc tggatgttca agagtttgat tatcccgagt ctttggatct tgctgcattg
    14101 gtttcgttgt tttcaaggca tggtatgcaa attttgggtc tgaagcattc taatgaacgt
    14161 tgggctgctg cggctatgaa gtatcatttg ctgttttgtc tgtctcattc ggaaaatgtt
    14221 aaagaactgg gtcaggttga ggtgcagaaa acggaggatg gtcagaaagc aaggaaaaca
    14281 gtattgatta catcccctgt ccgtaccggt cagcaggttt atgccgaaga tggcgatttg
    14341 attgttacgg gggcggtcag ccagggggcg gaattgattg cggatggcaa tatacatatt
    14401 tatgcgccga tgagggggcg tgctttggcc ggtgccaagg gtgatacttc tgcccgcata
    14461 tttatccact ccatgcaggc agaactggtt tctgtggcgg gtatttaccg taattttgaa
    14521 caggatttgc cgaaccatct gcacaagcag ccggtacaga tattgttgca ggataaccga
    14581 ttggttatca gtgcaattgg ctcagagtaa ttgtttgata tttaaaaagg aaatattgtg
    14641 gcaaaaatta ttgtagtaac ttcaggtaag ggcggtgtcg gtaaaacgac taccagtgcc
    14701 agtattgcga caggtttggc attacgcgga tataaaactg cggtaattga ttttgatgtg
    14761 ggtttgcgta acctcgacct cattatgggt tgcgagcgtc gtgtcgttta tgacctgatc
    14821 aatgtcattc agggggaggc gacgctcaac caagctttga ttaaagataa aaattgtgaa
    14881 aacctgttta ttttgccggc ttcccagact cgggataaag acgctttgac acgcgagggc
    14941 gtagaaaaag tgatgcagga gctgtccggc aagaaaatgg gctttgagta tattatttgc
    15001 gactctcctg ccggtattga gcagggtgca ttgatggcgt tgtattttgc tgatgaagcc
    15061 attgtaacga ccaatcctga ggtttccagt gtgcgtgact ccgacaggat tttgggaatt
    15121 ttgcaaagca aatcccataa ggcagagcaa ggcggttcgg ttaaagaaca tctgttgatt
    15181 acgcgttatt ctcccgaacg tgtggcaaaa ggcgaaatgc tgtctgtaca ggatatttgc
    15241 gatattctgc atattccttt gctgggtgtg attcctgaat cccaaaacgt cttgcaggca
    15301 tccaattccg gagaaccggt catccatcag gacagcgtgg cggcttccga ggcatataag
    15361 gacgttattg cccgtctttt gggcgagaac cgtgaaatgc gtttcttgga agctgagaaa
    15421 aaaagcttct tcaaacgtct gtttggagga taaggtatgt cattaatcga atttttattc
    15481 ggcagaaagc agaaaacggc aaccgttgcc cgcgaccgcc ttcaaatcat cattgcccaa
    15541 gagcgcgccc aagaaggtca ggctccggat tacctgccga ctttacgtaa agagttgatg
    15601 gaagtcctgt ccaaatatgt gaatgtttca ttagacaata tccgtatttc ccaagaaaag
    15661 caggatggta tggatgtgct tgagttgaac attactttgc cggaacagaa aaaggtatag
    15721 gacatgacct taaccgaatt gcggtacatc gtcgcagtcg cccaagaacg tcatttcggc
    15781 agggcggcgc ggcgttgttt tgtcagccag cccactttgt ctattgccat taagaaattg
    15841 gaagaagagc ttgccgtctc tttgtttgac cggagcagta acgatattat tacgaccgag
    15901 gcgggggaac gtatcgttgc acaggcgcgt aaggtattgg aagaggcgga gcttatcagg
    15961 catttggcaa atgaagaaca aaacgagctg gagggtgcgt tcaaactcgg gctgattttt
    16021 acggttgcgc cgtacctgct gccgaaactg attgtttcgt tgcgccgtac tgcaccgaaa
    16081 atgcctttga tgttggaaga gaattacacg catactttga ccgagtcgct caaacgcggg
    16141 gacgttgatg cgattatcgt tgccgaaccg tttcaagagc cgggcattgt taccgaaccc
    16201 ttgtatgacg aaccgttttt cgtgattgtc ccgaaagggc attcatttga ggaactggat
    16261 gccgtttcgc cccggatgct gggtgaggag caggttttgc tgctgacgga aggcaactgt
    16321 atgcgggatc aggtactctc aagctgttcc gaattggcgg cgaaacaacg tatacagggg
    16381 ttgaccaata cattgcaggg cagctcgatt aatacaatcc gccatatggt tgccagcggt
    16441 ttggcaatca gcgtgttgcc ggcaaccgca ctgaccgaaa acgatcatat gctgttcagc
    16501 attattccgt ttgagggtac gccgccaagc cggcgggtcg tattggcgta ccgccgcaat
    16561 tttgtccgtc cgaaggcgtt gtcggcgatg aaggcggcga ttatgcagtc gcagcttcac
    16621 ggggtaagtt ttatctgcga ctaggcgcag gcattgtttt caaaacgcca tttccctgag
    16681 ccgacaacac ggtatgccaa gatattgccg tcatcatcga ttttgagtat agcatcgcca
    16741 cggaaactgc cgtcctgaag atattcgact tttgcatcac tgtgaatgtt ttcatcagtg
    16801 ccgatgcaat gcca
//
LOCUS       DAWISC010000028        16614 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWISC010000028 DAWISC010000000
VERSION     DAWISC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.028"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(123..458)
                     /locus_tag="WIW87_000641"
     CDS             complement(123..458)
                     /locus_tag="WIW87_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ5173839.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WIW87_000642"
     CDS             complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WIW87_000642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ5173840.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQENNDIPPWEDSPDETAAGTAQA
                     SAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPSENPIQATPNNEALETEAFAH
                     EAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADAEEENNADESSNNEDHTPYAP
                     PPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADKKR
                     LDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPAAQ
                     KILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1424..1463)
                     /gene="dnaX"
                     /locus_tag="WIW87_000642"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WIW87_000643"
     CDS             complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WIW87_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ5173841.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQNDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGEDFQT
                     ASLGIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4867..5052
                     /locus_tag="WIW87_000644"
     CDS             4867..5052
                     /locus_tag="WIW87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ5173842.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5063..5731
                     /locus_tag="WIW87_000645"
     CDS             5063..5731
                     /locus_tag="WIW87_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ5173843.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5731..6444
                     /locus_tag="WIW87_000646"
     CDS             5731..6444
                     /locus_tag="WIW87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ5173844.1"
                     /translation="MKIKLPLFIIWLSVSASCASVSPVPAGSQTEMSTRENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHSSGRAYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYKSGKFSAAASLLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            6492..6977
                     /gene="purE"
                     /locus_tag="WIW87_000647"
     CDS             6492..6977
                     /gene="purE"
                     /locus_tag="WIW87_000647"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ5173845.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(7160..8614)
                     /locus_tag="WIW87_000648"
     CDS             complement(7160..8614)
                     /locus_tag="WIW87_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ5173846.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8611..9312)
                     /locus_tag="WIW87_000649"
     CDS             complement(8611..9312)
                     /locus_tag="WIW87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ5173847.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9309..10088)
                     /locus_tag="WIW87_000650"
     CDS             complement(9309..10088)
                     /locus_tag="WIW87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ5173848.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10373..11779
                     /locus_tag="WIW87_000651"
     CDS             10373..11779
                     /locus_tag="WIW87_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5173849.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            11891..13417
                     /locus_tag="WIW87_000652"
     CDS             11891..13417
                     /locus_tag="WIW87_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ5173850.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGDIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     CIDWQSVRTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPAYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            13625..14158
                     /locus_tag="WIW87_000653"
     CDS             13625..14158
                     /locus_tag="WIW87_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ5173851.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14201..15502)
                     /gene="aroA"
                     /locus_tag="WIW87_000654"
     CDS             complement(14201..15502)
                     /gene="aroA"
                     /locus_tag="WIW87_000654"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ5173852.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            15898..16374
                     /gene="greA"
                     /locus_tag="WIW87_000655"
     CDS             15898..16374
                     /gene="greA"
                     /locus_tag="WIW87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ5173853.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3493 a         4482 c         4638 g         4001 t
ORIGIN      
        1 tgatgccgtc attcccgcga aggcgggaat ccagacccct cggcacggaa atttatcgag
       61 taaaacggtt tcttagattc tacgttctag attcccgcct gcgtgggaat gacggtcggg
      121 gatcagcgga agaagtcgcc cactccgggg ggtagacctt gcgtgaatgc gcccattgtt
      181 ttgtttgcgg tttcttcggc tttgcctcgg gcggatttga gggcggcgag gatgaggtct
      241 tcaagcattt ctttgtcgtc ggcggcttct tgaatcaaat cggggctgat gtcgattttg
      301 cgtacttcgt gcgcgcaggt cattgtgatt ttgaccaggc cgttgcctgc ttcgccttcg
      361 atttcggttt cggcgagttt ggcttgcgct tttttcatat tttcctgcat ttgctgcgcc
      421 tgtttcatca ggccgcctaa tccggctttt ccgaacatac tgaatactcc tgtctgtttt
      481 gattgaataa gggaaacggc aatgccgtcc gaagggttcg ggcggcatta tatctgttta
      541 tggccggttt gccgccaatt ccagtgattc gggctgccat tgcgcgccga atgcttggag
      601 gattttttgt gcggcggggt cggcttcgag caatgcttgt gctttttgcc tgtcttcggc
      661 ttggacgcgc ttgtcctgca tcgcgggggt ttcccggccg gcttcgtcac gccagtcttg
      721 ggtttgcagg gtgagttgca gcccgtaggc ttgggcaagg gtgtcgcgga ttttgtcgag
      781 gcgttttttg tcggcggtgg cgcgtgcttc ggcggtcatt gccaaaacca tcagaccggt
      841 gtcgggatgg tattccgtcc acgcggagtg ttgcgccggc atttgcgccg cgccgagttt
      901 gcgggcgaag tgccggacga tggctgccca gttttcggtg gaaaattcgg gcggcggggc
      961 gtatggcgtg tggtcttcgt tgttgctgct ttcgtcggcg ttgttttctt cttccgcatc
     1021 ggcaggggcg gcgtgttccc aatcgggcgg tgggatttct gcgccgtctt ctacgaggta
     1081 gtcatcattc ggaaagctgt aaccgttgaa aggttttgca ggagcttcgt gtgcaaatgc
     1141 ttctgtttca agggcttcat tattcggtgt tgcctgaatg gggttttcag acggcacttc
     1201 ggacaagggg gcatcggttt cgttgtcggc tgcttcgttc ttggaaactt ggtttttggg
     1261 cggcgttccg gcttcggatg ccgtctgaat gctttttgcc gatgcttgcg ccgtgccggc
     1321 tgcggtttcg tccggcgagt cttcccaagg cggaatatcg ttgttttctt gattggtaac
     1381 gggttcggca gttttgcctt cagacggcat tgctgctgcg gatgccgtct gaacgggtgt
     1441 ttgggcggtt tccgcttcag ggcgcggttg gggctttttt gcggcggttt ccttttcggc
     1501 ggtttgtgcc gatggggatt ttagttcggt attttcaatc acggcatttg catcacacga
     1561 tgctgccgcc aagggcgcaa acgccagcat acgcagcagg gtcatcataa agccggcgta
     1621 ttcgtcgggg gcgaggctca ggtcgcgttt gccgtggacg gcgatttggt agtaaagctg
     1681 gatttgttcg ccgcttatgg tttgggcgag gcggtgcaaa atatcggaat cggggtcgtc
     1741 gtgcgccaag gcattcggca ctgcctgtat cagggcgagg tgttgcagca gtatggcaag
     1801 ttcgcccaag gcgttgtcaa agccgacggc acacgccgcc atttcctgcg ctttggcggt
     1861 cagggctgcg ccgtcttggt tgatgatgcc tgtcagcagt tcgtaaaggt attgtttgtc
     1921 aaccgcgccg atcatttggc ggacatcgtt ttcggcaact ttgcccgaac ctagggcgat
     1981 ggcttggtcg agcaggctca aggcatcgcg catcgatccg gcggcggcac gtcccaaaag
     2041 ttgcagggcg gcgggttcgt aggcgatttt ttcgctgtcg aggacgtggg cgaggtggtc
     2101 ggcaacctgt tgcgcggtca tattgcgtaa gacgaattgc aggcagcggc tcaagacggt
     2161 aacgggaact ttgtgcggat cggtggtggc gaggatgaat ttgacgtgtt cgggcggctc
     2221 ttccagcgtt ttgagcatag cgttgaacgc gcttttggaa agcatatgca cttcgtcgat
     2281 gatatagact ttgtattttc cggcggtcgg tgcatattgg gcgttttcca agacttcgcg
     2341 gatgttgtcg atgcctgtgt tggaggcggc gtcgatttcc agcaggtcga cgtagcgtcc
     2401 ggcatcgatc tgcgtacagc tttcacatac gccgcaaggt tcgccgtgtt gcgcgttttc
     2461 gcagttgagg cttttggcaa ggatgcgggc gatggtggtt ttacctacgc cgcgcgtgcc
     2521 ggtcagcagg taggcgtggt gcagcctgcc ttcgtccagg gcgttttgca gggctttgac
     2581 gacgtgttcc tgaccgacta agtcggcaaa ggttttgggc cgccattttc gggcgagaac
     2641 ttgataggcc atgtttttct cttggtttcg gtcgtgatgt ttctgtcggt gcgtcggaat
     2701 gccgtctgaa cggcggtctc gggcggcgta ttctagcact ttcggcttac tgtccgcgca
     2761 agaacagtgc gtccaattct ttcaaagtca gtttgaccca agtcggcctg ccgtggttgc
     2821 actggttgct gcgcggcgta ttttccatat cgcgcagaag ggcgttcatt tcgggcaggg
     2881 tgagccggcg gccggcgcgg atcgagccgt ggcaggacat ggtggcgagg atgcggtttt
     2941 cgtgttcctc gatggtttgg ctgctgccga cttgggcgag ttcgtttaat acgtctttgg
     3001 cgagcgagac gacatcggct ttgccgagca tggcgggaac tgcacggacg gcgagggtgt
     3061 tgccgcccat atcggataat tccagcccga agcctgccag cgtttcggca taatcggcaa
     3121 gggcggcgca ttcttcgtgg gacgcggcaa aggttacggg aataagcagg cgttggcttt
     3181 gcaggttgcc gttttcctga cgttggcgtt tcattttttc gtagttgacg cgttcggcgg
     3241 cggcgtgcat atcgatgagc aacaggctgt cttcggcttg ggcaagaatg tagatgccaa
     3301 gtaattgggc aatggcaaaa ccgagcggcg gcagttcgga ttgggacggg atgccgtctg
     3361 aaagcggcgt atctgtttgg ggagcaggcg tttcagacgg catattgccg aaacgtgcct
     3421 gctcgaatcg gcttaactca aggtcgatgt cgtcggtttt tttgtaaagt tcggcgtaag
     3481 tattcattgc cgcgcggctt tcgcgcaggg acaggctgcg ttgttgcggc gcatatgcgg
     3541 actgataggg catgggcgcg gttttgcctg atgaaccaaa ggcattgtgt gtatctgatt
     3601 tgttgcctgt cgggtagttg gatacgctat caaacagatt ttcgctgtcg ttttcagacg
     3661 gcattggggt ggagacaacg ccggtaatgt catgcaacac ttcgcctgcg ttgccgacgc
     3721 tttcggtcag gttggcgcgt gtgtcggcaa gggctttgtt gagcgtgtgg aacacaagtt
     3781 ggtgcacctg ctgactgtcg cggaagcgga tttcggtttt ggtcgggtgg acgttgacat
     3841 ccacggcttc gggcggcagg tcgagaaaga ggacgaaggc gggagtgagt gcgttgtgca
     3901 atacgtcgcg gtatgcctgc ttgacggcgt ggagcatcac tttgtcgcgc acgaagcgat
     3961 ggttgacgaa gcagtattgt ttgtcggttt tacctttggc gaaagtcggt ttggcaatcg
     4021 caccatagag ccgcagcgcg ccgttgccgc tgtcgattcc caatgatgcc gtctgaaagt
     4081 cttcgccgac aatggcggca atccgttcat gcaggctttg tgcagggagt ttgaacactt
     4141 gtttgccgtc gcgtttgagc gagaaggcaa tgtgcggatg cgccagcgcg aggcgttcga
     4201 gcatggtggc gcagtgggcg tattcggtgt tttcggattt gaggaacttg cgccgtgcgg
     4261 gggtgttgaa gaagagttcg gcggcttcga tggtggtgcc gacggggtgg gcggcggcgg
     4321 tggggctgct gagtttgccg tcttcggctt tgacttgggt cgcgtgcgaa ctgtcgttct
     4381 gacggctggt cagggtcagg cggctgacgg aggcgatgct tgccaaacct tcgccgcgaa
     4441 agcccatact ggcgacgtgt tccaaatcgt ttaaggtttt gattttgctg gtggcgtggc
     4501 ggtggagcgc aagttcgatg tcgtcggggt ggatgccgcc gccgttgtcg ctgacgcgaa
     4561 tcaggcggat gccgccgccc gccagctcga cttcaatcgc cgttgcgcct gcatcgatac
     4621 tgttttcaac gatttctttc aaggcgttgg cagggcgttc gaccacttcg ccggcggcga
     4681 tttggttgac aagatggtcg ggcagggcgg cgattcggga cataaggcgg gcttccgttg
     4741 caaaaaacgt cttattctat aataaaaccc cttatctttc tgcccgtatt cttgataaaa
     4801 gccgtttatc ccgtttggaa aataccagta taatcacgcc atattccgta aaattggagc
     4861 acaaagatgt atcactacca atccgatgcc acacaattcc tcaaccgcct gattgaagaa
     4921 aaacctgagt tggaacagca gcgtttggaa aacagggggc ttttgtggga tgtcgaactc
     4981 aatcccgaag aacaggaaaa cttcgaggcg gcaaaagtgg cgaaaaaacc ttatacctat
     5041 taccaagact gaatccgccg aaatgacgac ccatctgtcc aatgtcgcac cggacctgca
     5101 aaactatttg aacgccatcg gcgaacccga acatcccgtt ttgacgcggc tgcgcgagaa
     5161 gaccgggcat caccgtatgg gcaaaatggc gattgcgcgc gaacaggcgg cagttttggt
     5221 ttggctggca aagctgatcc gtgcggaaaa atatctggaa atcggcgtat ttaccggata
     5281 cagcagcacc gcgcttgcat tggcactgcc cgaacacggg cggattaccg cctgcgacat
     5341 caatgtaacc tttaccgata cggcgcgtca ggtttggaac gaggccggtg tggcacataa
     5401 aatcagcctg cacctgcaac ccgcattgct gacattggat gatttgattg cacagggtga
     5461 agccggaagc tacgatttgg cactgataga cgcagacaaa ccgcccacgc cgcaatattt
     5521 cgagcgttgc ctcaaactcg tccgtcaagg cggcatcatc gccatcgaca atattttgct
     5581 gaacggaagg gtgatgcgcg aagcggcttc cgatgcgccg cccagcgtcg gcatcctcaa
     5641 agatttcaat caaaacctgc cgaacgaccc gcgcatcgtc cccatcaccc tgcccgtcgg
     5701 cgacggcttg accctgcttc tgaaaaaata atgaagatca aattaccgct ttttatcatt
     5761 tggctgtctg tgtccgcctc ctgtgcttcc gtttcacccg ttccggcagg cagccaaacc
     5821 gaaatgtcga cacgggaaaa tgcttcagac ggcattccct atcccgttcc gaccttgcaa
     5881 gaccgtttgg actatctgga aggcaaaatc gtccggctgt cgaacgaagt ggaaacctta
     5941 aacggcaaag tcaaagcact ggaacacgca aaaacacatt cttccggcag ggcatacgtc
     6001 caaaaactcg acgaccgcaa gttgaaagag cattacctca ataccgaagg cggcagcgca
     6061 tccgcacata ctgtcgaaac cgcacaaaac ctctacaatc aggcactcaa acactataaa
     6121 agcggcaagt tttctgccgc tgcctccctg ttgaaaggcg cggacggagg cgacggcggc
     6181 agcatcgcgc aacgcagtat gtacctgttg ctgcaaagca gggcgcgtat gggcaactgc
     6241 gaatccgtca tcgaaatcgg agggcgttac gccaaccgtt tcaaagacag cccaaccgcg
     6301 cctgaagcca tgttcaaaat cggcgaatgc caatacaggc ttcagcaaaa agacattgca
     6361 agggcgactt ggcgcagcct gatacagacc tatcccggca gcccggcggc aaaacgcgcc
     6421 gccgcagccg tgcgcaaacg atagtttttc catttttccg ctttttaccc catccgcacc
     6481 aaggaggatt tatgatacaa atcggcatca tcatgggcag caacagcgat tggcccgtta
     6541 tgcggcaggc agcgcagttt cttgaagagt tcggcgtaga atatgaggcg cgcgttgttt
     6601 ccgcacaccg caccccggat ttgatgttcc aatacgccga aaccgcacgg gcgcgcggca
     6661 tcaaagccat catcgcgggt gcgggcggcg cggcgcattt acccggtatg gttgccgcca
     6721 agaccaccgt ccccgttttg ggcgtacccg tccccagcaa atacctgcgc ggcgaagatt
     6781 cgcttttatc gattgtacaa atgcccaaag gcgtacccgt cgccacattc gccatcggcg
     6841 aggcaggcgc ggcaaatgcc gcattgttcg ccgtttccat gctcgccaac gaaacccccg
     6901 aactggcgca aaaactggca gacttccgag ccaaacagga acaggctgtt ttgaatatgg
     6961 aattggaaca aatttaaaac ccaccgtctg cgaatatata gtggattaaa tttaaaccag
     7021 tacggcgtta cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     7081 tgatttttgt taatccacta tacaaatgcc gtctgaagcc ctgtttcggg tttcagacgg
     7141 catttttgcg aacaactttt tatgcctgtt ctttttggcg cattttttct gccattagtt
     7201 cgtgcagggt tttcttcgcc ggtttcatcg gcacgcggtt ttgcgtccaa cccaactgtt
     7261 tgcgcggggt caggttgcgg aacttggtgg ctgcccaacc gaaggcgcgg taggttttgc
     7321 tgccgctgaa aataccgttg aatgtgcgcc acgccatttg ttcgccgaag gtatgcgatg
     7381 cgccttgccc ccggatgggg tgcggcacgg tttcggtcgg cgaacgttgc gcttcaacgc
     7441 gcaaacgctg catttgttcg gtaatcggga tgcgtaccgg acaaacttcc acgcacgcgc
     7501 cgcacatcgt gcaggcggtc ggcaggtcgc gagtggcatc caagcctaac aggtgcgggg
     7561 aaataatctc gccaatcgga ccgggatagg ttgtgccgta tgccgcgccg ccgatgcggg
     7621 tataaaccgg gcaatggttc atacacgcgc cgcaacggat acattgcagg gtgcggcgca
     7681 tttggtcttc ggcataagcc tggctgcggc cgttgtcgag cagaaccaag tgcatttctt
     7741 gcggaccgtc taattcttca ctgcggcgcg ggccggtaat catattgaaa taagtggtaa
     7801 tgttctgacc aatggcagaa cgcggcagca ggctgtacaa gggtgggatg tcggacaatt
     7861 tcgccaccac tttttcaatg ccggtaatag cgatatgcac gggcggtacg gtggtactca
     7921 agcgaccgtt gccttcgttt tccaccagac acagcgtacc tgtttcagca acggcaaagt
     7981 ttacgccact caaaccgaca tcggcagtgc tgtaaatatc gcgcagtgct ttacgggcga
     8041 agccggtcag ttggtctaca tcgtctgtca gcggcgtacc gaggttttgg tggaacagtt
     8101 cgctaacctg ttctttggtt ttgtggatag caggcatcac gatatgggtc ggtttttcgc
     8161 ctgccatttg gacgatgaac tcgcccaagt cgctttctac cgctttaatg ccttttgctt
     8221 caagataatg gttcagctcg atttcctcgc tgaccatcga tttgcctttg accatcagct
     8281 tgccgttttt ggctgtgatg atgtcgtgga taatttggca ggcttcggtc ggggtttctg
     8341 cccagtgcac tttcacgccc aacttagtca ggttttcttc cagctgctcc agcagggctg
     8401 gcaatttaga caatgagcgc tgacggacgt gttcgcacaa atcgcgcagg ctttgcagct
     8461 cttcttcgtc ggtcaaaacg gctttgcgtt tggtcatcag catatccatc gcggtacgca
     8521 ggcttttgcg caaaggcttg tcttgaaggg aaattgcggc gttttgcttg aaagtttccg
     8581 gcttcatgtg aaatttgatg gtttgcgtag tcatgcgttt tcctccaaat cggcagggga
     8641 aatgtggtcg ggcaggatgg cgaggatgac caaatcgcgc gggccgtgcg cgccgtaagc
     8701 aagcgtcagt tggatgtctg cggttttgga cgggccggaa atcaggaata cattggtcgg
     8761 cataccgttt tccaccagtt tttcgccttc gacggcatta tgaaactcgt tgtacatctt
     8821 ggacgtatcg aacaggcaga aatgcacggg cggaacgagg cttaaagtac gcggttcttc
     8881 ggggctggaa aacagcatca gcgtgccggt gcgggcgatg ccgcattgcg cgccgctgaa
     8941 gcccgcatcg atgttcgtga aaaactcggt tttccaagta tcgatttcgc gctcgaaggc
     9001 aatcggttcg atattgctgt ccgccaatgc ggcacgggca atttgtccgt gttcggtcgc
     9061 caagggcagc aggatgtttt tcaaaccctt gccttctgcc gcttcgcgga aaacctgcat
     9121 ccaattgctt ttcgtcaccc aataaatttc ggttttgacc gcacgcatag cggcagccca
     9181 atgtttcaga cgctcaactt cgctgcccca agaaacaccc atttcacggt aataatcaaa
     9241 aaccgcaggt tcttccatcg gcaatgcgtc ggcttttttc agttttgcca aaatattttc
     9301 acgcgcgctc atgctttgcc tccggtgcgt tccaacaaga aggatgcgat atgtttcgga
     9361 cgcggcatat ccggctcgtc cttggcgatt ttgccgccga tgttcatcat acagccgcag
     9421 tccgcgctga tgatttcggt tgcgccggtt tctttcagcg cggcgacttt gtctgttacc
     9481 attgcgccgg aaatatcggc ttgtttgacg gagaatgtgc cgccgaagcc gcaacattcg
     9541 ctttcgtggt cgtggacgat gcgttcgacg ttttccatac cgtcaatcag ttgccagcct
     9601 gaaagatgga cattcatttc gcggcgggcg gcgcaggaag tgtgaacggc gactttgagc
     9661 ggttcgccct tgtcttcggg tttgaaaccg atggcaagca ggaaatgggt aaactcgatg
     9721 atgcggccgg cgcaatccac agccctttcc tcgtactcgc tgcctttaaa cagcgtcggc
     9781 cagtggtgtt tcatcatgcc gccgcacgag ccggacggca cgacgatcgg ccagttttca
     9841 gggaaaaggt cgagttgtgc tttggcgaca tcgaaggctt cggtcggatg gccggatgaa
     9901 taggcaggct ggccgcagca gctttgcgcc atcgggaaat ggacgcgtat gccctgctgc
     9961 tcgattaggg taatggcatc catgcctgct tcgggcataa aaagatcaag gacgcaagtg
    10021 ccgaaaaaat agacatcggt cggattgctg tcgtatcgga tgatttttgg gggaatggtt
    10081 gcgctcatgt tttttgataa cgggaaaccc gtttttctct gtagaaaggt aagcgtttac
    10141 tttaagtaat tgactgttgc gggtcaagtc taattttaaa aaataatccg gtttttctta
    10201 caaactgccc ccataacgct tactgtacct taatctgatg gttttcgata ataattatca
    10261 ttacaatgca atgccggttc gtttgcttgt gaacattcaa gatgccgact ctgacggcat
    10321 tcagacagca tctgaaaaca ataacggcac aaggatgggg tacaatcgcc ctatggaaaa
    10381 aaacacccta tccgcccgcg caccatcccc ttggctgccg cttctgctgg caattgccat
    10441 ttttatgcag atgttggatg cgaccatttt aaataccgca ctgcctgaaa ttgccgccga
    10501 cctgaatgag tcgcctctgg atatgcaact ggcagttatt tcctacacgc tgacggttgc
    10561 cctgctgatt cctttgagcg gttatttggc ggacaggttc ggaacgaaaa aagtcttttt
    10621 cggttcgatt gccgttttta tgctcggatc ggcattgtgc gccgcatcgg gttcgctgtt
    10681 tgaattgacg ctttcccgtg tcgttcaggg catcggcggt tcgatgctgg ttccgatacc
    10741 gcgtctgacc atcttgcgtg tgtacgacaa gtccaagctg ctcaatgcca tcaattatgc
    10801 ggttatgccc gcattaatcg ggccggtttt agggcctttg gcgggcggtt atttggtcga
    10861 atacgcttcg tggcactgga ttttcctgct caacctgccc atcggtctgc tgggtttcat
    10921 attgggacgc aacatcatgc ccgatattaa aggcagtaat atctctttag acttcaaagg
    10981 ttatctgatt ttttctgccg ccgcgtgcct cttgttactt tcggcagaaa gcctgtcgca
    11041 cgcgctgcct ccgtattttg cactgttgcc gctgtgcggc ggactgctgt ttgcacgccg
    11101 ttatttccga catatgaaaa ccgcgtccaa accgatttat tccgccgacc tgtttctgat
    11161 acgcactttc cgtctgggac tggcgggcaa tctgttcagc cgtctcggca tcagctcgat
    11221 tccttttctg atgcccctga tgtttcaaat cgctttcggc ttcggcgcaa gcctgtcggg
    11281 ttggctggtc gcacccgtcg ccctgtcttc gctgctggtc aaaccgctga ttgcaccgct
    11341 catgaaacgt ttcggctacc gcacggtact gctttggaac accaagctgc ttgccgcctt
    11401 catcatgctg ctcgccctgc ctgacggaaa ctcgccgctg tggatttggg ttttcctctc
    11461 gctggcgatc ggcgcgtgca actccctaca gttttctgcc atgaacacac tgaccctcgc
    11521 cgatttgcgc ccgcaacaaa caggcagcgg caacagcctg atggcggtca accaacagct
    11581 tgccatcagc atgggcattg ttgccggcgc attaatcctt aaaaactgga catttctgat
    11641 accggcttct tcaggtctgc attccgcctt ccgtatgacc ctgctcagca tcggcggcat
    11701 cacccttgca tcatcgctgg ttttcaaacg gctgcacgtt tcagacggca ccaacctgac
    11761 acggaacaca ccgtcctgaa gcggtccaca cgcaaaactt ttacccgttt caacgtttgg
    11821 attatgatac cgcacttcca tgcgcgccaa ccccaaaaca caggcaatgc cgtctgaaac
    11881 catatccctg atgaaaacac gcagcctaat ttccctttta tgcctccttc tctgttcatg
    11941 ttcttcatgg ttgcccccac tggaagaacg gacggaaagc cgtcatttca atacttccaa
    12001 acccgtccgc ctggacaaca tcctgcaaat ccggcacacc cctcatacca acgggctatc
    12061 cgatatctat ctgttgaacg acccccacga agcctttgcc gcccgcgccg cccttatcga
    12121 atctgccgaa cacagcctcg atttgcaata ctacatctgg cgcaacgaca tttccggcag
    12181 gctgctgttc aacctcgtgt accttgccgc agaacgcggt gtgcgcgtac gcctgctgtt
    12241 ggacgacaac aacacgcgcg gattggacga cctcctgctt gccctcgaca gccatcccaa
    12301 tatcgaagtg cgcctgttca accccttcgt cttacgaaaa tggcgcgcac tcggctacct
    12361 gaccgacttc ccccgcctca accgccgcat gcacaacaaa tcctttaccg ccgacaaccg
    12421 cgccaccata ctcggcggac gcaatatcgg cgacgaatac ttcaaagtcg gtgaggacac
    12481 cgttttcgcc gatttggaca tcctcgccac cggcagcgtc gtcggcgaag tatcgcacga
    12541 cttcgaccgc tactgggcaa gccattccgc ccacaacgcc acgcgcatca tccgcagcgg
    12601 cgacatcggc aagggtcttc aagcactcgg atacaacgac gaaacgtcca gacacgcgct
    12661 cctgcgctac cgcgaaaccg tcgaacagtc gcccctctac caaaaaatac agacaggatg
    12721 catcgactgg cagagcgtcc gaacccgcct catcagcgac gaccctgcaa aaggactcga
    12781 ccgcgaccgc cgcaaaccgc cgattgccgg gcggctgcaa gacgcgctca aacagcccga
    12841 aaaaagcgtc tatctggttt caccctattt cgttcccaca aaatccggca cagacgcact
    12901 ggcaaaactg gtgcaggacg gcatagacgt taccgttctg accaactcgc tgcaggcgac
    12961 cgacgttgcc gccgtccatt ccggctatgt caaataccga aaaccgctgc tcaaagccgg
    13021 catcaaactc tacgagctgc aacccaacca tgccgtcccc gccacaaaag acaaaggcct
    13081 gaccggcagc tccgtaacca gcctgcacgc caaaaccttc attgtggacg gcaaacgcat
    13141 cttcatcggt tcgttcaacc tcgacccccg ttccgcgcgt ctcaacaccg aaatgggcgt
    13201 tgttatcgaa agccccaaaa tcgcagaaca gatggagcgc acccttgccg ataccacacc
    13261 cgcctacgcc taccgcgtta ccctcgacag gcacaaccgc ctgcaatggc acgatcccgc
    13321 cacccgaaaa acctacccga acgaacccga agccaaactt tggaaacgca tcgccgcaaa
    13381 aatcctatcc ctgctgccca tagaaggttt attatagaaa tatagcggat taacaaaaac
    13441 cagtacgacg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
    13501 aagtgaatcg gtttcgtact gtttgtactg tctgcggctt cgtcgccttg tcctgatttt
    13561 tgttaatcca ctataccgtc tgaaacacct tcagacggat atccgaaccc gcaaaggaaa
    13621 aaccatgttt ccccccgaca aaaccctttt cctctgtctc agcgcactgc tcctcgcctc
    13681 atgcggcacg acctccggca aacaccgcca accgaaaccc aaacagacag tccggcaaat
    13741 ccaagccgtc cgcatcagcc acatcgaccg cacacaaggc tcgcaggaac tcatgctcca
    13801 cagcctcgga ctcatcggca cgccctacaa atggggcggc agcagcaccg caaccggctt
    13861 cgattgcagc ggcatgattc aattcgttta caaaaacgcc ctcaacgtca agctgccgcg
    13921 caccgcccgc gacatggcgg cggcaagccg caaaatcccc gacagccgcc tcaaggccgg
    13981 cgacctcgta ttcttcaaca ccggcggcgc acaccgctac tcacacgtcg gactctacat
    14041 cggcaacggc gaattcatcc atgcccccag cagcggcaaa accatcaaaa ccgaaaaact
    14101 ctccacaccg ttttacgcca aaaactacct cggcgcacat acttttttta cagaatgagc
    14161 cgcccgccgc gccgcgccgg aatcgcggca aaatgccgcc ttattccgct gtttcggtca
    14221 gcgatgagaa cacgtcgaaa taagtcggga aggttttgtg ggtgcatttc ggatcgttga
    14281 tgacgacggg tacgcccaac agcgaaacca gcgagaaaca catcgccatg cggtggtcgt
    14341 cgtacgtgtc gatgacggcg tcgggtgtca gcgtttcggg cggggtgatg tgaattgctt
    14401 cggcttcttc gacgactttt gccccgagtt tgcgcaactc gtttgccatt gcggcgatgc
    14461 ggtcggtttc tttgacgcgc cacgaaccga tgttgcgcag cgtgcaggtt tgccctgtag
    14521 caagcgcgac gatggcgagg gtcatggcgg catcggggat atggttcgca tccaaatcaa
    14581 aggattggac ggcacgttcc ttcgggcgtg aaacttcgac gaagttttcg ccccaaacca
    14641 cgtccgcccc gattttttcc agctcgcggg caaaggcgac atcgccctgt atgctgtttg
    14701 cgccgatacc ggtaacgcgg acgggcgtgg cggcaatcaa accggctgcg aggaagtagg
    14761 acgcgctgga ggcatcgcct tcgacgtgca agtgttcggg cgcgtggtag tgcgcatcgg
    14821 cgggaatttt gaagacgcgg tagccttcat tgataacctg tacgccgaat tgcgccatca
    14881 gttttaaagt aatgtcgata tagggcttgg aaatcaattc gccgaccata cggatttcaa
    14941 acgcctgccc ggtcagcggc aacgccatta aaagggcggt cagaaactgg ctggacacat
    15001 tgcctttaat cggaatcacg cgctcgccgt tgtcttggcg ttcgccgata tgaagcggcg
    15061 gatagtgttc cttgccgaga tattcgacat cggccccggc aatccgcaac gcatcgacca
    15121 aatcgccgat aggacgttcg tgcatacgag gcacgccgtg cagatgataa tcgccgccca
    15181 aaacggccag agcggcggtt aacgggcgga acgccgtgcc cgcgttgccc aaaaacaaat
    15241 cggcagtgcg gttggggaag cgtccgcctg tgccgtgcac tttcagacgg tcttcggcaa
    15301 gatattcgat ttgaacgccg agtttatcga gtgcttcgag catacggtcg gtatcgtcgg
    15361 atttgagcag ggaatggatt tcgcaagcat tgtcggacaa ggcggcaagc agcagggtgc
    15421 ggttgctgat gcttttggag ccgggcaggg cgacggtgga aggtttgaga cgggcgacgg
    15481 ggaggcggac ggattcggtc atggcaaaac gtcaatatta ttaaagataa aaacagcctg
    15541 cattatactg gtgcaaatgc tgtatgaaaa atctcaggct tggcattttc ggttttaaag
    15601 tccgtaaatg tggtttttta tgccgaaaat tgattatttt taaatttttt gtttctaaaa
    15661 tttctttgtc ggcatatttt cagcttttgt tgcggacatg gcgcgtttgc cggcggcgcg
    15721 gcacgattgc caatttcata gaatttggta gaatagccgc tgttcaacga cagacaagcc
    15781 gccgattttc cgggcggctt gtatttttat tttaatatcc ggacgcacaa aacaagacgg
    15841 tacgcggtat gcttgctgcc gcaaaccggt gtgccggaat atggagaaaa aagaccgatg
    15901 caaaaaatcc ccctgaccgt acgcggtgcg gaattgctga aacaggaatt gcagcagctc
    15961 aaaagcgtgg cgcgtcccga agtgatcgaa gcgattgccg aagcccgttc gcacggcgat
    16021 ttgtccgaaa acgccgaata cgaagccgcc aaagaacgcc aaggctttat cgagggccgc
    16081 atttccgagc tggaacacaa actttccgtt gcccacatca tcaatccgac cgaaatccac
    16141 gccgaaggca aaatcgtgtt cggtacgacg gttacgctgg aagatttgga aacggaagaa
    16201 cacgttatct atcaaattgt cggcgaagac gaagccgaca tcaaacaggg caaaatctat
    16261 gtcggctcac ccatcgcccg cgccttaatc ggcaaggaag agggggatac ggcggaagtc
    16321 caagccccgg gcggcgtgcg cgaatacgac attatcgaag tccgatatat ttgattcggc
    16381 ttgatttcga tacacccgac acacgcagga aattatagtg gattaataaa aatcaggaca
    16441 aggcgacgaa gccgaagaca gtacagatag tacgaaaccg attcacttgg tgcttcagca
    16501 ccttagagaa tcgttctctt tgagctaagg cgagacaacc ccgtactggt ttttgttcat
    16561 ccgctataac agcaaccctg tcgccgtcat tcccgcaaaa gcgggaatct agga
//
LOCUS       DAWISC010000029        16367 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWISC010000029 DAWISC010000000
VERSION     DAWISC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.029"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            125..1189
                     /locus_tag="WIW87_000656"
     CDS             125..1189
                     /locus_tag="WIW87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ5173854.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVAEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATINVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1330..1653
                     /locus_tag="WIW87_000657"
     CDS             1330..1653
                     /locus_tag="WIW87_000657"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ5173855.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLVEKA
                     ARTES"
     gene            1725..2048
                     /locus_tag="WIW87_000658"
     CDS             1725..2048
                     /locus_tag="WIW87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ5173856.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQMLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2095..2298
                     /gene="tatA"
                     /locus_tag="WIW87_000659"
     CDS             2095..2298
                     /gene="tatA"
                     /locus_tag="WIW87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ5173857.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2302..2988
                     /gene="tatB"
                     /locus_tag="WIW87_000660"
     CDS             2302..2988
                     /gene="tatB"
                     /locus_tag="WIW87_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ5173858.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3002..3772
                     /gene="tatC"
                     /locus_tag="WIW87_000661"
     CDS             3002..3772
                     /gene="tatC"
                     /locus_tag="WIW87_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ5173859.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3825..4433
                     /gene="maf"
                     /locus_tag="WIW87_000662"
     CDS             3825..4433
                     /gene="maf"
                     /locus_tag="WIW87_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ5173860.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4442..4795
                     /locus_tag="WIW87_000663"
     CDS             4442..4795
                     /locus_tag="WIW87_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ5173861.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4785..6599
                     /locus_tag="WIW87_000664"
     CDS             4785..6599
                     /locus_tag="WIW87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ5173862.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6789..7466
                     /locus_tag="WIW87_000665"
     CDS             6789..7466
                     /locus_tag="WIW87_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ5173863.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7480..8886
                     /locus_tag="WIW87_000666"
     CDS             7480..8886
                     /locus_tag="WIW87_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ5173864.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9004..11430
                     /locus_tag="WIW87_000667"
     CDS             9004..11430
                     /locus_tag="WIW87_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ5173865.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASEQPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLVSFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLHAHAPKNLTLHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLAHPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11475..11720
                     /gene="rpsP"
                     /locus_tag="WIW87_000668"
     CDS             11475..11720
                     /gene="rpsP"
                     /locus_tag="WIW87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ5173866.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11736..12245
                     /gene="rimM"
                     /locus_tag="WIW87_000669"
     CDS             11736..12245
                     /gene="rimM"
                     /locus_tag="WIW87_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ5173867.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12245..12994
                     /gene="trmD"
                     /locus_tag="WIW87_000670"
     CDS             12245..12994
                     /gene="trmD"
                     /locus_tag="WIW87_000670"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ5173868.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13010..13375
                     /gene="rplS"
                     /locus_tag="WIW87_000671"
     CDS             13010..13375
                     /gene="rplS"
                     /locus_tag="WIW87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ5173869.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13696..14421
                     /locus_tag="WIW87_000672"
     CDS             13696..14421
                     /locus_tag="WIW87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ5173870.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMQRFADRQIAYLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14445..15320
                     /locus_tag="WIW87_000673"
     CDS             14445..15320
                     /locus_tag="WIW87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ5173871.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15397..16311
                     /locus_tag="WIW87_000674"
     CDS             15397..16311
                     /locus_tag="WIW87_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ5173872.1"
                     /translation="MKHLKLTLIAALLTASATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAKALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4236 a         5249 c         3607 g         3275 t
ORIGIN      
        1 tcttgtagta aaatatctaa acagattcaa ctggaaacgg tcaagcttct tcacgccatt
       61 cccatttcaa ccgccgcctg ccagattcag acggtattcc tacttgaaaa caaggagcgt
      121 tacaatgaaa gcagcacgtt tttacgacaa aggcgacatc cgcatcgaag acatccccga
      181 accgaccgtc gcccccggca ctgtcggcat caatgtcgcc tggtgcggca tctgcggtac
      241 tgacctgcac gaattcatgg aaggcccgat tttcattccg ccttgcggtc atccgcaccc
      301 gatttccggc gagtccgcac ccgtaacgat gggacacgag ttctccggcg tggtctatgc
      361 cgtcggcgaa ggcgtggacg acatcaaagt cggccaacac gtcgtggtcg aaccctacat
      421 catccgcgat gacgtaccga ccggagaagg cagcaactac cacctctcca aagatatgaa
      481 ctttatcggc ttgggcggct gcggcggcgg tctgtccgaa aaaatcgccg tcaaacgccg
      541 ttgggtgcat ccgatttccg acaaaatccc gttggatcaa gccgctttga tcgaaccgct
      601 gtctgtcgga caccacgcct atgtacgcag cggcgcgaaa gaaggcgacg tcgcattggt
      661 cggcggtgca ggtccgatcg gtttgctgtt ggctgccgtg ttgaaagcca aaggcatcaa
      721 agtcatcatc accgagttga gtaaagcacg caaagacaaa gcgcgcgaat ccggcgttgc
      781 cgactacatc ctcgacccgt ccgaagtcga tgttgttgca gaagtgaaaa aactgaccaa
      841 cggcgaaggc gtggacgtgg catttgagtg caccagcgtc aacaaagtgt tggatacttt
      901 ggtcgaagcc tgcaaacctg ccgccaattt ggttatcgta tccatctgga gccaccccgc
      961 caccatcaac gtccacagcg tcgtgatgaa agagttggac gtgcgcggca cgattgccta
     1021 ctgcaacgac cacgccgaaa ccatcaaact ggtcgaagaa ggcaaaatca accttgagcc
     1081 tttcatcacc cagcgcatca agctggacga gctggtttcc gaaggcttcg agcgtctgat
     1141 tcacaacaac gaatccgccg ttaaaattat tgtgagtcca aacctgtaag caaattacat
     1201 ttgcaacagc acaaatgccg tctgaaacgc tcagacggca ttttccaacg aagcgcaaca
     1261 ggcgtattgc ccgagagcag ccgtttagcc tatcattccg aacaaacaga aacaaacaga
     1321 aggaaaatca tgggagattc cgtactatcc gccatccaac aaaccatcac ccagcgaaaa
     1381 tctgccaatc cgtccgaatc ttacgtcgca cagctcttgc ataagggcga agacaaaatc
     1441 ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga caaaaacccg
     1501 tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct tctgacacac
     1561 cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgtcaggg gctgtcgggg
     1621 ctggtcgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg cactttccca
     1681 cattcaatcc gtctgaccgc ttttcagacg gcatcggagc cgttatggac aactgtattt
     1741 tctgcaaaat cgccgccaaa gacattccgg cgcaaaccgt ctatgaagac ggcgaaatgg
     1801 tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt cccaaagtcc
     1861 atttcgattc gttggcacac gccgcgcccg aacatcagat gctgctgggc aaaatgatgt
     1921 tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa accctgatca
     1981 ataccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc atgggtacac
     2041 ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata catcatgggc
     2101 agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat attcggcacc
     2161 aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt caaacagggg
     2221 ctgaacgaag gtacagacgg caaagaagcc caaaaagacg atgtaatcga acacaaaaaa
     2281 gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt ttttgtcggc
     2341 attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg caccgccgga
     2401 cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt tgacactcaa
     2461 atcgaactgg aagaactgag gaaggcaaag caggaatttg aagctgccgc cgctcaggtt
     2521 cgagacagcc tcaaagaaac cggtacggat atggaaggca atctgcacga catttccgac
     2581 ggtctgaagc cttgggaaaa actgcccgaa cagcggacac ctgccgattt cggtgtcgat
     2641 gaaaacggca atccgcttcc cgatgcggca aacaccctat cagacggcat ttccgacgtt
     2701 atgccgtccg aacgttccta cgcttccgcc gaaacccttg gggacagcgg gcaaaccggc
     2761 agtacagccg aacccgcgga aaccgaccaa gaccgcgcat ggcgggaata cctgactgct
     2821 tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac tgctgttgaa
     2881 acgcctgttc cgcacaccac ttccctgcgc aaacaggcaa taagccgcaa acgcgatttt
     2941 cgtccgaaac accgcgccaa acctaaattg cgcgtccgta aatcataaag agagcaatcc
     3001 ggtgtccgaa acacaaaacg aacaacccgt ccaaccgctt gtcgagcatc tcatcgagct
     3061 gcgccgccgc ctgatgtgga cggttgtcgg catcttagtc tgctttttcg gcctaatgcc
     3121 gtttgcccaa caactctata cttttatcgc cgacccgctg atggcaaacc tgcccaaaga
     3181 caccagcatg attgccaccg atgtcatcgc accatttttc gtgccggtca aagttaccct
     3241 gatggcggca tttttaattt cgctgccgca tacgctctac caaatctggg catttgtcgc
     3301 gcccgcactc taccaaaacg aaaaacgcct gattacgccg ctcgtcctct ccagcgtcag
     3361 cctgtttttc atcggcatgg catttgccta ctttttggtt ttccccgtca ttttcaaatt
     3421 ccttgccagc gttacccctg tcggtgtcaa tatggcgaca gacatcgaca aatacctctc
     3481 cttcatcttg gggatgtttg ttgcgttcgg cacaacgttt gaagtcccca ttgtcgttat
     3541 cctgttaacc aaaattggtg tggtaacaac cggacagctc aaacgcgccc gcccctatgt
     3601 gattgtcggc gcgtttgtca ttgccgccat catcacgccg cccgatgtga tttcacaaac
     3661 cctgcttgcc attccgctga ttctcttata cgaagcaggt atttggttcg gacgcttttt
     3721 cacgccacgt tcagaacagg atggcgacat acggccgcct gcaacaacct gacactatgc
     3781 cgtccgaacc tccgcctcat accgcaacag attaaggaat acctttgaat accctctatt
     3841 taggttcaaa cagcccgcgc cgaatggaaa tcctgacaca gttgggctac cgcgtcatcc
     3901 aactgcctgc cggcatcgac gaatccgtta aagccggcga aacacctttc gcttacgttc
     3961 aaaggatggc agaagaaaaa aaccgaaccg ccctgaccct cttttgcgaa accaacggca
     4021 caatgcccga tttccccctg attaccgccg acacctgcgt cgtttcagac ggcatcatat
     4081 tgggcaaacc ccgctcccaa gccgaagcaa tcgaattttt aaaccgattg tccggcaaac
     4141 aacataccgt cctgactgct gtctgcattc attatcgcgg caaaacgtca agccgcgtcc
     4201 aaaccaaccg cgtcgttttc aagcccctga gttcggaaga aatttccgcc tatgtgcaaa
     4261 gcggcgagcc gatggacaaa gccggtgcct acgccgtaca aggcataggc ggcatcttta
     4321 tccaatctat cgaaggcagc ttcagcggca ttatgggact gcccgtttat gaaaccgttt
     4381 cgatgttgca ggatttggga taccgctccc ccttgtccgc ccttaaaccg taaagaccgc
     4441 cgtgaacaga caaaccgctt acctccttgc ctctttcagc ctgatcgcac tgataatcct
     4501 gtccctttcc tgggaactgt ggattgcacc gttgcgcccg ggcggctcgt ggctcgcgct
     4561 caaagccctc cccctctgtc tgccgctttc aggcatcttg aaaaagaaaa tctatactta
     4621 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4681 cgcctatccc gcagaacaga tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4741 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgccc gctgaaacga
     4801 ccgtatccgg cgcgcacccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4861 ggataggcat cgtccccttt accttcgcgc tcaaactgaa accgtcgccc gacttttacc
     4921 acgatgccgc cgccgcagcc ggcctgattg tcctgttgtt cctcacggca ggaaaaaaac
     4981 tgtttgatgt caaaatcccc gccatcagct tccttctgtt tgcaatggcg gcgttttggt
     5041 atcttcaggc acgcctgatg aacctgattt accccggtat gaacgacatc gtctcttgga
     5101 ttttcatctt gctcgccgtc agcgcgtggg cctgccggag cttggtcgca cacttcggac
     5161 aagaacgcat cgtgaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5221 gcatcgtcgt catccagttt gccggctggg aagacacccc tctgtttcaa aacatcatcg
     5281 tttacagcgg gcaaggcgta atcggacaca tcgggcagcg caacaacctc ggacactacc
     5341 tcatgtgggg catactcgcc gccgcctacc tcaacggaca acgaaaaatc cccgccgccc
     5401 tcggcgtaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaac tcgcgcacca
     5461 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5521 aatccaacag gcggacgatg ctcggcatag ccgcagccgt attccttacc gcgctgttcc
     5581 aattttccat gaacaccatt ctggaaacct ttactggcat ccgctacgaa actgccgtcg
     5641 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aatcgaatgg aataaagccc
     5701 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5761 ccttcctcat caatgccgaa cagcacaaca tatacgacaa cctcctcagc aacttgttca
     5821 cccattccca caacatcgtc ctccaactcc ttgcagagat gggaatcagc ggcacgcttc
     5881 tggttgccgc aaccctgctg acgggcattg ccgggctgct taaacgcccc ctgacccccg
     5941 catcgctttt cctaatctgc acgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6001 ctttgtggta tgtctatttc ctcatccctt tcggactgat gctcttcctg tcccccgcag
     6061 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6121 ccgccatatt cgcaggattg ctgcacttgg actggacata cacccggctg gttaacgcct
     6181 tttcccccgc cactgacgac agtgccaaaa ccctcaaccg gaaaatcaac gagttgcgct
     6241 atatttccgc aaacagtccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6301 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaatcactaa
     6361 aataccgccc ccactccgcc acctaccgca tcgccctcta cctgatgcgg caaggcaaag
     6421 ttgcagaagc aaaacaatgg atgcgggcga cacagtccta ttacccctac ctgatgcccc
     6481 gatacgccga cgaaatccgc aaactgcccg tatgggcgcc gctgctaccc gaactgctca
     6541 aagactgcaa agccttcgcc gccgcgcccg gtcatccgga agcaaaaccc tgcaaatgac
     6601 ccgcgccggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6661 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6721 cgtcaaacaa tcttttcacg ccccgcccga aacagggtcg gggcataccc ttacgaaaag
     6781 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctaacc gaactgctga
     6841 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     6901 tacaggaaat cctgagcggg cagtacgatg tagtcgtatt ggattccatg atgcccaaaa
     6961 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7021 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggc gcggacgact
     7081 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctgcgcc
     7141 gcgcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7201 tcctgtaccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7261 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7321 ccctgtccgt cgaagcactc gaccgcaagc tggcaaaatt cgaccgcagt atcgacgtac
     7381 acatctccag catccgccac aagttgggcg atgcctctct gattcaaacc gtacgcggct
     7441 tgggctacct gtttgtcaaa aactgaaata aacagataaa tgaaactgtt ccaacgcatt
     7501 ttcgccacat tttgcgcggt tatcgtctgt gcaatctttg tggcgagttt ttctttctgg
     7561 ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac gccgcaccat cgaaaccact
     7621 ttgatgggca gcatcatttc cgcattccgg gcacgcgggg acgcgggtgc gcgcgaaatc
     7681 ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt acgttataca gggcgacgag
     7741 aaaaaagata tcctgaaccg gtatatcgac agctatacca tcgaacgcgc ccggcttttc
     7801 gccgccggac acccgcattc caacctcgtc catatcgaat acgaccgctt cggcgaagaa
     7861 tacctgttct tcaccaaaga ctgggacaaa ctccaagccc gccgcctgcc cagccccctg
     7921 ttgatccccg gcctgccgct cgccccgatt tggcacgaac tcatcatatt gtccttcatc
     7981 atcatcgtcg gactgctgat ggcatatatc ctcgccggca acattgccaa acccatcaga
     8041 atcttaggca acggcatgga cagggtggca aacggagaac ttgaaacccg tatctcccaa
     8101 caggtcgacg accgcgacga cgaattgtcc catcttgcca tccaattcga caaaatggtg
     8161 gaaaaactcg aaaaactcgt tgccaaagaa cgccacctgc tccatcacgt ctcccatgaa
     8221 atgcgttctc cccttgcgcg catgcaggca attgtcggac tgattcaggc gcagccccaa
     8281 aaacaggagc aatatctcaa acggctggaa ggcgaactga cccgcatgga tacgctggcc
     8341 ggggaactgt taaccctgtc ccgtctcgaa acttccaata tggctttgga aaaagaaagc
     8401 ctgaaactcc tgcccttcct gggcaacctg gtagaagaca atcaaagcat tgcccagaaa
     8461 aacggacaaa cggttaccct gtctgccgac ggaaaaatcc ccgaaaacac aaccatcctt
     8521 gccaacgaaa gctacctgta ccgcgccttc gacaacgtca tccgcaacgc cgtcaactac
     8581 agtcccgaag gcagcaccat cctgatcaac atcggacaag accacaaaca ctggataatc
     8641 gacgttaccg acaacggccc cggcgtggac gaaatgcagc tcccgcacat cttcaccgct
     8701 ttctaccgtg cagactccag tgccaacaaa cccggaacag gactggggct tgcattgacc
     8761 caacatatta ttgaacagca ctgcggcaaa atcatcgccg aaaacatcaa accgaacggt
     8821 ctgcggatgc gctttatcct gcccaagaaa aaaaccggtt ccaaaacaga aaaaagtgcg
     8881 aactgaccat aataccgtct gaaccggctt cagacggcat tgcacaaaca gttaccccaa
     8941 aacaaacaca cagcgggtgt ctctccaaaa atcacgcaca taccgtccgc aaaggaatat
     9001 atcatgtcgg cacaaaccga tccgggctac ttcttcatgc ccaaccacat catcctgata
     9061 ggcgcgagcg aacaaccgta cagcctgggc gaacgtgtac tcagcaacct gctgagtacg
     9121 ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc acaccatagc cggactgccc
     9181 gcctacacca gcctcaacaa aatccccggc aatgcagacc tgattattgc cgttacccta
     9241 cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa agcagctccg acacatcatc
     9301 ctcatacagg actgggacaa cctgtctgcc gcagaactgc acaccgccga aactgccatc
     9361 cgcaaacacc acggcaacgg actcaacatc accgcctgca ccaccgcagg catccaactg
     9421 ccctcactcg gactcaacat cagtacccaa gacggatacg ccgcaggcca taccgccata
     9481 ctgaccggca atgccgccgt cagccgccaa atcgacaaca tcctgaacaa actccgtcaa
     9541 ggcacatccc gccacatcag cctgcatccc ggcatcagcc ccatcacatc cgccgattgg
     9601 ctcaaccgct tcggacacag cctgcacacc aaaaccgccg tcctacacca caaccctgaa
     9661 gaggatcagc gcaaactgtt cagcgcaatc cgccaattta cccgccatac gccgctgatt
     9721 ctccacatca cctgccgcac gacagaaacc gaccgtgccg tactgcactg cctcgcccga
     9781 cactgcaact tcctcgtcag tttcaacgcc gacgacctcg aagccgcact gcgcgcccaa
     9841 ctgtccgacc ttccacccct gtcccgactc gacatcctgt ccgacacgcc tgccgaatgg
     9901 ctgcacgcgc acgcgccaaa aaacctcacc ctccactttc ccaaccttcc ccaccacatc
     9961 cgcaacggac acctgaccgg cacacccaca ccctcaatct gccacgacat cgcctcacgt
    10021 cagcttgccc accccgacac ccaagccgtc ctaaccatcc tcagtccctc cggacacgag
    10081 gattacaaaa aaacagcacg cgcccttatc cgcctgtccg aacagaccgc caaacccctg
    10141 ctcgtcagca gccccttttc agacggcata acccatttcg acacccccac tcaggcaatc
    10201 cgcacccttt cctaccgcaa caccgccgcc gccctgaaac aggcacagct cgacattgca
    10261 ccgccgcagc catgccgtct gaaaacaccg caaccccaaa acatcaaaaa agcccttgca
    10321 gcggcaaacc cctccctgct cgccgaagcc ctgcacctcc ccccctaccg gcacaccacc
    10381 cataacgccg tacaattcca attcgacagc caccccctct atggcgacat cctgaccgca
    10441 cgctgcaacg gacaaaccac tgccgtactc ccgccgttta ccacgctcga cagccgccac
    10501 cttgcccgct ttgccgaact cgacggcaca caaaccctcg accagttcct gcacacactg
    10561 accgtcattc ccgaataccg ccaacacatt ctcggcatca ccctcaacct caacggcgga
    10621 caatacagca gcgatttcct cttaaagaca cccgaaacac acgacacgcc caaacgcaag
    10681 aacacaggca aagccgccca aaccctcgaa catgccgccg caaaaatgca gagtgccgcc
    10741 gcatacctga aacacaaaaa cccgacagcc gccgaatttc tccgccacac aagcgaagcc
    10801 gccgcagaac tgctcggcag caaaaccgaa accggagcag ccgtacccaa cgtacttgcc
    10861 ccctatcccg cagcataccc caaaacactg tccctaagaa acaacacgac cgttaccatt
    10921 acccccattt tgcccgaaga cgcagaagcc aaacagcagt tcgtccgcag cctcggtccc
    10981 gaagcacggt acacacgctt catgacccac accaacgaac tgcccgcagc cacgttggca
    11041 cgcctgtgca accccgatta ccactgtgaa gccgcatgga cggcaaagga tgccgacagc
    11101 aacatcgtcg ccgtcgtccg ccacagccgc ctgaatcgca acgaatgcga attcggcatc
    11161 acactggcgg aacatatgcg cggcagcgga ctggcacaga aaatgatgga actcatcatc
    11221 caaaccgccg cacagcaagg ctaccggact atgagtgccg acattctcaa aaccaatacc
    11281 cccatgatca aacttgctga aaaatcagga tttaccctca aggaatcgga caccgaaaaa
    11341 aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa caacagaaaa aacaaataaa
    11401 aacttgcgca ccgaccacaa aataacctaa aatcggcagt ttccatatat ccgattttct
    11461 caaaaggact caaaatggta gttatccgtt tggcacgcgg cggctcgaaa caccgcccct
    11521 tctacaacgt catcgttact gactcacgca gccgccgcga cggccgcttc atcgaacgcg
    11581 taggcttcta caaccccgta gccaacgaaa aacaagagcg cgtccgcctc aatgcagacc
    11641 gcctgaacca ctggattgca caaggcgcgc aagtcagcga ctccgttgca aaactgatta
    11701 aagaacaaaa agccgcctaa tccgcatttg ccgccatgac agacactcaa aaccgggtag
    11761 ccatgggcta catcaaaggc gtattcggca taaaaggctg gttgaaaatt gccgccaaca
    11821 ccgaatattc cgacagcctt ttggactacc ccgagtggca tttggtcaag gacggcaaaa
    11881 ccatcagcgt tacccttgaa gccggaaaag tcgtcaacgg cgaactccaa gtcaaattcg
    11941 aaggcataaa cgaccgcgac ttggcattct cattgcgcgg ttacaccatc gaaatacccc
    12001 gtgaagcatt cgccccgaca gaagaagacg aatactactg gacagacttg gtcggcatga
    12061 ccgttgtcaa caaagaccat accgttttag gcaaggtaag caacctgatg gaaaccggcg
    12121 caaacgacgt attgatgatt gacggagaac acgggcagat tctgattccg ttcgtttccc
    12181 aatatatcga aaccgtcgat accggcagca agaccattac tgccgactgg ggtttggact
    12241 actgatgctt atccaggcag ttaccatttt ccccgaaatg ttcgacagca ttacccgcta
    12301 cggcgtaacg ggacgcgcga acagacaggg aatctggcag tttgaagcag tcaatccccg
    12361 aaagtttgcc gacaacagat tgggctacat cgacgaccgc ccgttcggcg gcggcccggg
    12421 aatgattatg atggctccgc cgcttcatgc ggcaatagaa cacgccaaaa cacaatcctc
    12481 ccaagctgca aaagtcatct acctcagccc ccaagggaaa ccgctgacac accaaaaagc
    12541 ggtagaactg gcagaacttc cgcatctgat tctgctgtgc ggacggtatg agggcataga
    12601 cgaaaggctt ctgcaaagca gcgtcgatga agaaatcagc atcggagact tcgttgtttc
    12661 cggcggagag cttcccgcca tgatgctgat ggatgcggta ttgaggctcg tacccggcgt
    12721 attgggcgat atgcagtctg ccgaacagga ttcgttctca agcggcattt tggactgccc
    12781 ccactacacc aaacccttag aatttcaagg tatggctgtt ccggaagtat tgcgttccgg
    12841 caatcatggc ttgatagcgg aatggcggtt ggaacaatcg ctgcgccgca ccttggagcg
    12901 cagacccgat cttttggaaa agcgcgtttt aatcccaaag gaatcccgcc tcttagaaac
    12961 catccggcaa gagcaacggg aaatccaatc ataatttagg aaaaaaacaa tgaacctgat
    13021 tcaacagctc gagcaagaag aaattgcccg cctgaataaa gaaatccccg aattcgcacc
    13081 gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt acccgcagcc gtctgcaagc
    13141 ctacgaaggc gtggttattg cccgtcgcaa ccgtggtctg aacagcaact tcatcgtccg
    13201 caaaatctcc agcggcgaag gtgttgaacg tactttccaa ctgtactctc cgaccgtcga
    13261 aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc aaactgtact acctgcgcgg
    13321 cctgaccggt aaagctgcac gcatcaaaga aaaactgcct gcacgcaaag gttgattcaa
    13381 accgttttcc cccaatgccg tctgaacctt cagacggcat ttcttattgc tgcgatgccg
    13441 gcaagtcaac gatatgcctg cccctcatcc accttgatta acaatcactc ccgcctattc
    13501 aagcataatt tacagaccaa acgttataca gtatcatatc agccatataa cgataaacgc
    13561 tttaccgcct tccctttcaa aaccgcaaat catcatgaca ccttcccttt tactatcagg
    13621 attgaccttc cgcctcatcc ttgccctgat tgccgtatcc cttttatggg gcgtttactt
    13681 ttgggcagta tccgcatgag catcattgtc gaaaacctga ccgtcagcta ccgccgccga
    13741 cctgccgtgc accatgtgga cattactttt gaagaacata gtatgtgggc ggttttcggt
    13801 cccaacggcg cagggaaatc cacctttctc aaatccttga tgggattgca gcctatcgat
    13861 acaggcagca tccggctgga cggattgacc cgtcagaaca tcgcctacct tccccagcag
    13921 tccgatatcg accgctccca gcctatgacc gttttcgact tggcggcaat ggggctatgg
    13981 tatgaaatcg gctttttcaa agggataaat accgctcaaa aacaacgcgt tcacgaagcc
    14041 ttggagcgcg tcggaatgca acggtttgcc gaccgtcaga ttgcctatct ctcaaacgga
    14101 caatttcagc gtgtcctttt tgcccgaatg ctggttcaaa atgccaaatt cctgctgctc
    14161 gacgaaccct tcaatgccgt tgatgcacgg acaacctacg agcttctcga cgtattgcag
    14221 aaatgccatt gcggcggaca cgccatcatc gccgtactgc acgattacga acaagtccgt
    14281 gcctactttc ccaataccct gctgctcgcc cgcgaaaaaa ttgcggcagg cgcaaccgag
    14341 accattctga cagaacccta cctcgcccaa gccaacgcca aaatgcagca acaggaaagc
    14401 cccgactggt gcgcctcata aatgccgtct gaaaccgaaa aaccatgaat ctctacgacc
    14461 tgctccttgc cccctttgca gaattcgact ttatgcgcta cgccctcgca tccgtcttct
    14521 gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt atgagcctga
    14581 taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac atgtttgccg
    14641 gcttgagcct gcccgccatg ggtttgggcg gcgtagccgc aggcatgctg atggcactgc
    14701 ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt gccgcctttt
    14761 atctcagcag cctcgccatc ggcgtagtcc tcgtcagcaa aaacgggagc agcgtcgatt
    14821 tgctccacct ccttttcggc tctgtacttg ccgtcgatat tcctgccctg cagctcatcg
    14881 ccgccgtctc cagcctcacg ctcattaccc ttgccgtcat ctaccgcccg ctcgtactcg
    14941 aaagcatcga ccccctgttt ctcaaatccg tcggcggcaa aggcgggctt tggcacgtcc
    15001 tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc ggcacactca
    15061 tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctgtgggcg aagcatatgg
    15121 gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc ggactgctca
    15181 tttcctacca catcgaaatt ccttccggtc ccgccatcat cctctgttgc agcgtccttt
    15241 atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg ctcaaaaacc
    15301 accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcggc ttgttttacc
    15361 ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa actcaccctt
    15421 attgccgcat tgctgaccgc ctccgcaact gccgcccccc tgccggttgt aaccagcttc
    15481 agcattttag gcgacgtagc caaacaaatc ggcggagagc gcgtatccat acaaagtttg
    15541 gtcggagcca accaagatac gcacgcctat catatgacca gtggcgacat taaaaaaatc
    15601 cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagctgccga tgtgcaacgc
    15661 gccgtcaaac aaagcaaagt atcctatacc gaagcgacca aaggcatcca acccctcaaa
    15721 gccgaagaag aaggcggaca ccatcacgac cacgatcatg accacgaagg acaccaccat
    15781 gaccacggcg aatatgaccc gcacgtctgg aacgaccccg tccttatgtc cgcctatgcc
    15841 caaaacgttg ccaaagccct gataaaggcc gatcccgaag gcaaagttta ttatcaacaa
    15901 cgcttgggca actaccaaat gcagctcaaa aaactgcaca gcgacgcaca agccgcattt
    15961 aatgccgtcc ctgctgccaa acgcaaagtc ctgaccgggc acgatgcctt ttcctatatg
    16021 ggcaaacgtt accatatcga attcatcgcc ccgcaaggcg tgagcagcga agccgagcct
    16081 tcggccaaac aagtcgccgc catcatccga caaatcaaac gcgaaggcat caaagccgtc
    16141 tttaccgaaa acatcaagga cacccgtatg gttgaccgta tcgccaaaga aaccggtgtc
    16201 aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg cgcccgcaga cacctacatc
    16261 ggaatgtacc gccacaacat caaagccttg accaacgcga tgaagcaata aaaccgtttg
    16321 attggtttga atttgaaaag gcaaaaggcc gtctgaaaat tatagtg
//
LOCUS       DAWISC010000030        16173 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWISC010000030 DAWISC010000000
VERSION     DAWISC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.030"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WIW87_000675"
     CDS             complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WIW87_000675"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ5173873.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARVDTAATAAAEAPAAAPAEAAPAAAPAA
                     TQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAGAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1327..4155)
                     /locus_tag="WIW87_000676"
     CDS             complement(1327..4155)
                     /locus_tag="WIW87_000676"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ5173874.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WIW87_000677"
     CDS             complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WIW87_000677"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ5173875.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5755..6003)
                     /locus_tag="WIW87_000678"
     CDS             complement(5755..6003)
                     /locus_tag="WIW87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ5173876.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6007..6714)
                     /locus_tag="WIW87_000679"
     CDS             complement(6007..6714)
                     /locus_tag="WIW87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ5173877.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIW87_000680"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIW87_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ5173878.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYQGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIW87_000681"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIW87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ5173879.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIW87_000682"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIW87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ5173880.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9373..9573)
                     /locus_tag="WIW87_000683"
     CDS             complement(9373..9573)
                     /locus_tag="WIW87_000683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173881.1"
                     /translation="MPSEIFSDGILFLGMGRMLIPCREGGGKTKNLSFNLLFPRVFVK
                     IYQFVFKIHCSKWDKTGKLTFM"
     gene            complement(9590..10993)
                     /locus_tag="WIW87_000684"
     CDS             complement(9590..10993)
                     /locus_tag="WIW87_000684"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5173882.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11242..11979)
                     /locus_tag="WIW87_000685"
     CDS             complement(11242..11979)
                     /locus_tag="WIW87_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ5173883.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWSAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12258..14534)
                     /gene="metE"
                     /locus_tag="WIW87_000686"
     CDS             complement(12258..14534)
                     /gene="metE"
                     /locus_tag="WIW87_000686"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ5173884.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14670..15548)
                     /gene="metF"
                     /locus_tag="WIW87_000687"
     CDS             complement(14670..15548)
                     /gene="metF"
                     /locus_tag="WIW87_000687"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ5173885.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15749..16024
                     /locus_tag="WIW87_000688"
     CDS             15749..16024
                     /locus_tag="WIW87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ5173886.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16024..16149
                     /gene="ykgO"
                     /locus_tag="WIW87_000689"
     CDS             16024..16149
                     /gene="ykgO"
                     /locus_tag="WIW87_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ5173887.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         3954 a         4086 c         4025 g         4108 t
ORIGIN      
        1 ttcagacggc cttttcatta acaaataaaa ggccgtctga aacgattaca gatccaacaa
       61 caggcgggcc gggtcttcca acgcgtcttt aatggctacc aaggtcaata cagcttcgcg
      121 gccgtcaatg atacggtggt cgtaagacag agccagatac atcatcggac ggacaacaac
      181 ttggccgttt tcaaccacag cgcgctcttt agtggcgtgc atacccaaaa tcgcagattg
      241 aggtgggttg atgatcgggg tagacatcat agaaccgaaa gtaccgccgt tggtaatact
      301 gaatgtaccg ccggtcagat cttcgatagc gattttgccg tctttggctt ttttcgcgta
      361 atcaacaatt gcttgttcga tgtcggcaat gctcatttgg tcggcatcac gcagaattgg
      421 cacaaccaaa ccgcgtgggc tgccaattgc gataccgatg tcgaagtagc cgtggtacac
      481 gatgtctttg ccgtcaacag aagcattcac aaccgggtat tttttcaggg cggcaacagc
      541 ggctttaacg aagaaggaca taaagcccag tttcacgccg tgttctttct cgaatttttc
      601 tttgtacttc gcacgcaagt ccatgattgg tttcatgttg acttcgttga atgtagtcag
      661 aatggcgttt tcttgttgag aagccaagag gcgttctgca acacgggcac gcaggcggct
      721 cattggtacg cgttcttcag gacgtgcgcc ggcaggaagt gcaacagcag gagcagcggc
      781 tccggcaggt ttggcagcgg cattttgtac gtcttctttc aatacgcgac cgtcacggcc
      841 ggaaccttgc aatgcgttca cgtcaacacc ggtctcggca gccagtttgg cggcagcagg
      901 catagcggcg ttgttttgtg tagcagcagg agcggcagct ggggcagctt ctgcaggagc
      961 ggcggctggg gcttcagcag cggcagtagc agctgtatcg acgcgcgcca aaacttggtc
     1021 ggcaacaacg gtttcaccgt cttgagctac gatttcaacc aatacgccgg cttgtggaga
     1081 aggtacttcc aaaaccactt tgtccgtttc gatatcgatc aggatttcgt cacgggcaac
     1141 ggcttcgcca acttttttct tccattccaa gagcgtgcct tcagatacgc tttcagacaa
     1201 cataggtact tttacatcaa taatcatttt gtgtctccaa tggccctttc agacggcctg
     1261 ttgtgtgttt ttaattctgc atatgggcat tccatctgag cagactcaga cggaatgcta
     1321 cttggtttac aatgccaaag cgtcttcaac caattgtttc aattgagcaa tgtgtttgct
     1381 tgagtagccc actgcaggcg atgcgctgct tggacgaccg gcataagaca gtttttgctc
     1441 ttcgctaata acatcttcga tgcggtggcg gatttggtag aacgcgcctt ggtttttcgg
     1501 ctcttcttgt gcccaaacca cagattttgc gttcggatat ttcgccagtt cagctttaac
     1561 ctcgtcatat gggaacggat acagctgctc aacgcggaca atagcaacat catcttccag
     1621 tttacgctcg gcacggccgg cttccaagtc atagtaaacc tgaccggcac acaataccac
     1681 gcgtttcacg ctgtcgttgc ttgcgcgttc tgcggtatcg ccgataaccg gacggaaggt
     1741 cgaaccttcg gtgaagtttt ccagcgggct cattgcacct ttgaagcgca acaggcgttt
     1801 ggacatgaaa atcaccagcg gtttgcggta tgaacccaag acttgacgtt gcaagaggtg
     1861 gaacatttgc gacgcttcag acggcataat gacttgcata ttgttctcag aacacagttg
     1921 caaccaacgt tctacgcgtg cagaagagtg ctcggggcct tgaccgtcgt agccgtgcgg
     1981 caggatggta gtcagaccgc acaaacgacc ccacttggtt tcgcctgaag acaggaattg
     2041 gtcaatagtc acttgcgcgc cgttggcgaa gtcaccgaat tgagcttccc aaatggtcag
     2101 tttgtcaggt gcggagcagg caaagccgta ctcgaacgcc atcacggctt cttcgttcaa
     2161 aatggagtcg ataaccagga actcgcccat gccttcgccc atatggcgca gaggaacata
     2221 agtaccgtcg tcccattttt cgcgtttttg atcgtgcaat acggcgtggc ggtgcgagaa
     2281 cgtgccgcgt cccgagtcct cgccggaaat acgcacgccg tgacctttgg ttacgaggct
     2341 ggcgtatgcc aaggtttcgg ccatacccca atctatggcc tgtttgccgg atgccatggc
     2401 tttacgcgct tcaatcacac gttttgcagt cggatgcagg gcaaagcctt ccggtacggc
     2461 ggtaaacttc tcagtgagac gttcaatatc tgcggcaggc aaaccggttt cgatgtgttc
     2521 gcgccaatct ttgccttggt atttgctcca gtcgatttgc gtgcgttgga agttgctcaa
     2581 cgttgtttgc tcgacgtgct cgcctttgtc caaagcatca cggtaagctt ggatgtaacc
     2641 gtcagcctcg gcttgggtta ccacgccttc ggcaatcagt tgctcggtgt acaaagcacg
     2701 cgcaccgggg tgttgcgata cttttttgta catcatcggt tgggtcaagg tcggatcatc
     2761 gccctcgttg tgaccccatt tacggtagca gacaacgtca atcacgatgt ctttatggaa
     2821 ttttttgcgg taatccaaag cggcttggat agcaaagcaa acgcgttcgg gatcatcgcc
     2881 gttcacatgg ataaccgggg cggaaaccat ttttgcgata tcggtacagt gtacggttga
     2941 acgggtatcg cggatatcgg aagtggtaaa gccgatttgg ttgttgatga cgatatgaac
     3001 cgtaccgccg gtggtataac cgcgcgtttt agacaggttg aatgtcgctt ggttgactcc
     3061 cagaccgata aatgcggagt cgccgtgaat caataccggc aagactttgt cgcggccgtt
     3121 ttcgcccaaa cgtttttgtt tggcgcgcgc agaaccttcc accaccgggt tgacgatttc
     3181 caagtgtgac gggttgaacg ccaaagaaac gtgcatcggg ccgtgcggcg tggcaatatc
     3241 ggagctgaag cccatatggt atttcacgtc gccgctgggc agtttgattt cggcacgacc
     3301 ttcaaattcg gcaaacaaat cgccgggttt tttgcccaaa atgttcacca aaacattcag
     3361 acggccacgg tgcgccatac cgatgatgac ctcttccaca ccgtctttac cggcgttttg
     3421 aatcaggtag ttcaaaccgg caatcgcgct ttcgccgcct tcgacaccga aacgtttctg
     3481 accgacatat ttggtatgca gataacgttc caaagtctcg gcagcagtca tctctttcaa
     3541 gatacggcgt ttttgatcgg cattgtaatg cggtgtggac aatacgcttt caaaataatt
     3601 gcgtacccag cggcgctctt cggtattggg aatatagata tattccaatg cgatgtggcc
     3661 gcagtaggtt tgtttgaggt tgctgatgat ttgggacaaa ggcagtttgc cgcgattggc
     3721 aaaatcgccc tcgcccatat tgaattgaag cgccatatcg gcatctgaca gaccgtggaa
     3781 tttcggatcg agggcttcaa tatcgcgcgg ggggatacgt ttgagcggat caagttgggc
     3841 tgcacccacg ccttggatac gataggcgga aatcagccgt aaaacgctga cttgcttttt
     3901 cagcattgcc tcatccgcac cgcccgcaac ggcagatgca attttctttt tcgccaaagt
     3961 aacaaatgat tcgcgaatcg gtgtgtgtgc gacatcgaca gcaaccgtcc ccggctgttt
     4021 gctcaaatcg gtgaaatact gcttccattt ttcatcaacc gcatcggggt tttccaaaaa
     4081 agcctcgtac aattcctcaa tgtaaggtgc gtttgaaccg aacaggtaag agaaattgag
     4141 tttttcgtcc atcatggcgt gcgctctttt ctttaaaata taaacaaagg gtagaagtcc
     4201 gcgtcctgac ggctaaactg atgccgcctg aaaagacatt ttattctacc ctattttaaa
     4261 ccggattgct tgtcttgatt tcatccgact acaatcagac ggattcggga aacaatccgg
     4321 ttctctgata ttgcctgttt gacaatatat taatgtatgt tacctctcgc ctggcggcac
     4381 ataatcgcgg cgttccgaac cggtataaag ctggcgcggg cggccgattt tcagcgaagg
     4441 atcgctaatc atctcgtgcc agtgcgaaat ccagcccacg ctgcgcgaca gggcgaagat
     4501 gacggtaaac atttcggtcg ggatgcccag cgcggacagg acgatgccgg aatagaaatc
     4561 gacgtttgga tacagtttgc gttcgataaa gaacgggtct ttcagcgcaa tctgttccaa
     4621 ttccatcgcc agtttgaatt tcggactgtc ttccaagccc aattccttca aaacttcata
     4681 gcaggtttcg cgcataatgc tggcacgcgg atccatattg cggtacacgc ggtgaccgaa
     4741 gcccatcaga cggtatttgc gttgtttcac accttccatg tatgcggcaa cattagacac
     4801 atcgccgatt tcgtccaaca ttttcaacac ggcttcgttc gcaccgccgt gcgaagcacc
     4861 ccacaggcag gcgataccgg cagcaataca ggcaaacggg ttcgcacccg aagaccctgc
     4921 cagacggacg gttgaagttg aggcgttttg ctcgtggtcg gcatgcaaaa taaagatgcg
     4981 gtcgagcgcg cgtgccaaaa cgggattggg tttgtagtct tcacacggcg tggcgaacat
     5041 catatgaagg aagttttcgg aataagaaag attattcttc ggataattga acggcagacc
     5101 gtttgaatag cggtagcaca ttgccgcaat ggtcgggatt ttagaaatca ggcggtaaat
     5161 cgcgattttg cggtgttcgg gattgctaat gtccaagctg tcttggtaga acgcagacag
     5221 tgcgccgacc acgccgacca tcatcgccat cggatgcgcg tcgcggcgga acccccggaa
     5281 gaaccaagtc agctgttcat gcaccatcgt gtggcggcgg acggtattgt caaattctgc
     5341 cttttgctcg ggagtcggca gttcgccgta aatcaacagg tagcagactt ccaaataatc
     5401 ggacttttcg gccagctgct cgatggggta tccgcgataa taaagcaagc cttgatcgcc
     5461 gtcgatgtaa gtaattttag actcacagct tgcggttgaa acaaatccgg ggtcgaagga
     5521 aaacaaacct gtattttttg tcagcccccg aatgtcaacc acatcgtgcc cgatgctggc
     5581 ttccaatacc ggcagctcca aacctgcctg acccggtacg ttgagtttga ttgatttgga
     5641 catattattg ctcctttgca agttataacc cacgttactg catccgcagc acgttctttg
     5701 ccttcagacg gcttttgcat tttgaaatct gcctgcaggc agatttcaga tgcctcatgc
     5761 ccgtctgatt ttttcaagca tcgggataag gtgccctttg tccgtttccg aatgcccgtt
     5821 aatcaaggca agcaattctt gatcttgaaa ttcaaggatt tcggaaaact cggacagctc
     5881 tttatcgctc aaatgctcga attctttttc cataaacctg ccgaagatta aatctaattc
     5941 caacaatccc cggcgggttt gaaaacggat tttccgtttg gcaatatcgt caaaaaccat
     6001 catttcttaa acggcccgtt tcaacataat ctctttaatc ttaccgatgg ctcgggtcgg
     6061 attcaagtgt ttaggacata cgtctacgca gttcataatg gtgtggcaac ggaacaaacg
     6121 gtatgggtcg ttcagattat ccaaacgctc attagtgatg gtatcacggc tgtccgcaat
     6181 gaaacggtaa gcattcagca aaccggacgg accgacgaat ttatcagggt tccaccaaaa
     6241 tgacgggcag gcagtcgaac agcaggcgca caaaatacac tcgtacaaac cgtccaactc
     6301 tttacgctct tcctgagttt gcagacgctc tttgtccgca tcaatcggat tatcgttgac
     6361 aacataaggt ttgacggaat ggtattgttt gaagaactgg gtcatatcca caatcaggtc
     6421 gcggataaca ggcagacctg gcagaggacg gattttaact ggctgtttca agccacgcag
     6481 atcggtcaaa cacgccaagc cgtttttgcc gttgatgttc ataccgtccg atccgcaaat
     6541 gccttcgcgg caggagcggc ggaaagacaa ggtatcgtct tgtgctttca ggcgtaccaa
     6601 agcatccaaa agtttcacgt cggtcggttc caattccaac tcgtaacgct gcatataagg
     6661 cttggcatca acatccgggt tgtaacggta aatttcaaaa ctcatttttt ccatgagaag
     6721 tgttcctttt ctcatcaact gcccaatgat tttgtattat aaattcaagc agttcaatgt
     6781 gggtatggtt agatttcaga cgacctttga ggcgaagact gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtgtgcac cggtttgtag
     6901 gacaaggtat tgatatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aatcaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acagagcgtt gcagttcgcg acgcagcgca tcgacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa caccaccaag
     7381 tccagcagag agttcgtacc caaacggttc gcaccgtgta cggaagcaca ggcgcactca
     7441 cctgcggcat acaggccttt tacaggcact tcgtattcgt ctccttgcgg cacaacgact
     7501 tcgccttggt aattggtcgg aatgccgccc atcatatagt gggtagtcgg cacaacagga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgctcca taaagcgttc gccgtcggcg
     7801 ttcaacaaaa taccgccttc accgcgtaca ccttcggtaa tcaacacgcc tgcacctgcc
     7861 acacctgtcg ggtggaattg ccagaactcc atatcttcca acggaatacc cgcacgcgcg
     7921 caaatgccca aaccatcgcc ggtattcata taggcattgg tagaagaagc ataaatacga
     7981 ccgccaccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cttcctgcac attacccaaa gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8881 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccgctgattc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccaaataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgaccca tccccaaaaa
     9541 caaaatgccg tctgaaaaaa tttcagacgg cattgttttg ccatatcggt caggctttca
     9601 atttcgcatc ggcatcgcgc aacgcggtac gcaaaccttc ctcgataacg ggatggtaga
     9661 acggcatatc cagcatttgc ggaacggtca tcttcatttg atgtgcccaa gccaacaggt
     9721 gcgccaaatg ttcggcggcc ggacctacga tttctgcgcc gatgaagcgg ccggtggctt
     9781 tttcggcata caggcgcata tggcctttgt ttaccagcat cacgcggctg cggccttggt
     9841 ttttgaacga tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg
     9901 cgtatttcaa accgacaaag ccgatttgcg gactggtgaa cactacgccg atggtgctgc
     9961 ggcgcaaacc gccgccgata ttcgggtagc ggcctgcgtt atcgccggca atcttgcctt
    10021 ggtcggcggc ttcatgcagc aggggcagtt ggttggacgc gtcgcccgca ataaagatat
    10081 gcggaatact ggtctgcata gtcagcggat cggcaacggg tacgccgcgc gcatcttttt
    10141 cgatattgat gttttccaaa ccgatattgt caacgttcgg acggcggcct acggctgcca
    10201 gcatatattc ggcaacaaat acgccttttt cgccatcctg ctcccaatgg acttctacat
    10261 tgccgtctgc gtcgagtttg gcctcggttt tagcatccaa atgcagtttc aattcttcac
    10321 cgaacacggc tttcgcctcg tctgaaacga cggggtcgga aatgccgccg atgatgccac
    10381 ccaaaccgaa aatttcaact ttcacgccca aacggtgcaa tgcctgaccc agttccaaac
    10441 cgataacacc cggtccgaac acggcaacgc gcttaggcag cgtatcccat gagaaaacgt
    10501 catcgttgat aatcaaacga ttgcccaaag actgccattg cggcaggatg acgggacgcg
    10561 aaccggtagc aatcacgaaa ctttttgccg taatttgggt atggtcgtcg atttggacgg
    10621 tatgctcgtc gataaactta gccgagccca tgatgcgctt gtcggcaggc cactcttcca
    10681 catcagcaac gacaaagccg acaaaacggt cgcgctcgga tttaacacgc cgcattactt
    10741 cttcaccgtt gacgacgatg ctgtctttgt ccaaatgcac gccgaacggg tcggtatgca
    10801 gtgcgtggtg gcgcgcttct gcggcggcaa tcaagagttt ggaaggcata caacccaccc
    10861 gcgcgcaggt cgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac
    10921 gggcattgcg aaacgcaccc ataccggcag taccgccgcc gattacgacg acatccgctt
    10981 gaattttttt cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca
    11041 tatcggtttc agacggcatt taaatatttt cccaatcaaa aacatcattc agataaataa
    11101 aaacaaaatt tcgattgagt gcaacaatat ccaaattctc ttataaatat agagttattc
    11161 ggagcggcag tgtagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa
    11221 accgccttaa gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc
    11281 gccgatgtat ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatggcgcg
    11341 aacggaagta acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc
    11401 ttgcaaagct tgtttggctt tagcgcagaa ttggcaacct ggtttagtga aaattgccac
    11461 agactcttga gccttccaat cgggagcaac gaattgcagc atagtatctg catcggatac
    11521 tttgaaagga tcgcccggtt cttcaggttc gatgaacatt ttttcaacca cgccgtcgtt
    11581 aaccagcatg gagtaacgcc aagagcgttt accgaagccc aagtcttctt taccgaccag
    11641 catacccata ccttcggtaa attcgccgtt gccgtcagga atcatgtaga tgttgtcgga
    11701 ttcttcttcg gcagaccaag cgttcattac gaacgtatcg tttacagata cgcagcagat
    11761 tgcgtcaacg ccgttttctt tgaacgcgcc gaacaattcg ttgtaacgcg gcaggtgtga
    11821 agaagaacaa gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt
    11881 gaacaaatca tcggtagaca catctttcca agtgtcgccg acacgggtgc ggaatactac
    11941 ggaaggtact ttttgaccgg tacgatcttg caaagccatt ttaagctcct taatagattg
    12001 gtttgagaaa taattgtgaa tcgctgggca ttctacccca tgcaaaatga tttgtataat
    12061 ttatagttca aataatataa attatttaaa actatcaaac acaactccga tttgcctgtt
    12121 tcccacatat aaagccgaat tagaaaaaaa caagctgcaa tgaaaagatt ttccggcaac
    12181 tgcgcccttg cgccgcaaat caggacaaaa taccgtctga aaggctttca gacggtattt
    12241 attcatacgg tctcggctta tttcgccaat tcggcacgca atttgtgggt tacgttcatc
    12301 atcacttgga gttgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg
    12361 ttaacccaca gacgttcaac cggtacaacc tcgatggctt tgcgcaacag gtgctccact
    12421 tcggcttctg tcggtacgcg cgggctgtgg atgtcgtaaa cccccgggcc gatgtcgttc
    12481 gggtatttga attcgccgaa cgcggtcaag agttccatgt cggaacgtga agtctcgatg
    12541 gtgatcacgt ccgcatccat tgcagcaatc gcaggcagga tatcgttgaa ctcggagtag
    12601 cacatatgag tgtggatttg tgtgctgtct tcgcaaccgg cagaggacag gcggaaggat
    12661 tcgcccgccc agttcaggta ggcatcccaa tcggcgcgtt tcagcggcaa gccttcgcgg
    12721 atggcaggtt cgtcaatttg gatgactttg atgcctgctt tttccagatc caatacttcg
    12781 tcgttcagag ccagtgcgat ttgtttgcac acggtagagc gaggaatatc gttgcggaca
    12841 aaagaccatt gcagaatggt tacagggccg gtcaacatac ctttcatcgg gcgtttggtc
    12901 aggctttgtg cgtaagtaga ccaggcgaca gtcatggctt caggacggct tacgtcaccg
    12961 aagataatcg gtggtttaac acaacgtgag ccgtagcttt gtacccagcc gtattgggtg
    13021 aatgcaaaac cgctcaacaa ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg
    13081 ccgtgtacca gtacgtccaa atccagtttt tcttgttctt caaccaccaa ggcgatttct
    13141 tttttcatcg cggcttcgta atcggcggca gacagttcgc cttttttgaa ggctgcgcgt
    13201 gcttggcgga tttcggtagt ttgcgggaaa gaaccgatgt tggtcgtcgg cagcagaggc
    13261 aggttcaacc atgcttgttg cgctttgata cggtcggcaa atggagattt gcgttggtct
    13321 gcgttggcag gcaaatcggc caggcgtttg gcaacgtctg cacggtggat ttcgctgctg
    13381 ttggcgcgtg aatcggcagc agcttggctg gcggccagtt cttcggcaac ggaatcgcga
    13441 ccttcgttca atgcggcttt cagaacgcgc aattcttggg ttttttgcag ggtaaatgcc
    13501 aaccaagagt acaggtcggg tttgttggct ttcagttttt cttcaactga caagtcaaat
    13561 ggagtgtgca gcagagagca agagctggag atccacaaac ggtcgcccag tttggcttgc
    13621 agaggctcaa cagtttccaa aactttgttc aggttggcgc gccaaatatt gcggccgtca
    13681 atcacgccgg cggacaggac tttgtcgtag tcggcgaacg cgtccagttg ctcgggagcg
    13741 cgtaccaagt cgatgtgcag accgtcaaca ggcagggctt tcagcaatgc ggcgtgttcg
    13801 gcaacagaac cgaagtaagt gctcaacagg attttggcgt tgactttgct caaagtagcg
    13861 taaacgtctt tgtaggcttc cacccattct ttaggcaggt caacagccaa agcaggctcg
    13921 tcgatttgaa tccattctgc accggcttca accaaagcgt ttaggatttc aacgtaaaca
    13981 ggcagcagtt tttgcaacag gctcaggcgg ttgaattcga ctgtgccttt ttctttaccc
    14041 acccacagga aagtcaacgg acctacaacg gtcggtttgg ctttcagacc gagggcttgg
    14101 gcttcttgca gttgttgaac atagtgtttg gcattggctt tgaattcggt atcggcgtgg
    14161 aattcaggca ccaagtagtg gtagttggtg tcgaaccatt tggtcatttc gatagcgaat
    14221 tggtctttgt tgccgcgcgc cagttggaag aattgttcca aagacaggtt ttggctgtcg
    14281 aagccgaagc gggcgggaat cgcgccggtg gcgacttgca ggtcgaggat gtggtcgtag
    14341 aaagtgaaat cacctacggc aacgaaatcg gcgttggcag cggcctggtg tttccagttt
    14401 ttctcgcgca agtctttagc aacagccagc aattcttgct cgctgatttc tttgcgccag
    14461 tatttttctt gtgcgaattt caattcgcgg aaggcgccga cacgcgggaa gcctgagaaa
    14521 tgtaatgttg tcatgttgaa ctctccagtt ggattttgta aggaccggtt cgatccgaat
    14581 cggtcggtat tgtaaatcag aatggctcgg atggggcggc gttttgcttt tcagacggcc
    14641 tcgtatgccg tctgaagccg ttttcggctt taagggcgca cgcctaaaat atggcaaatg
    14701 gcgtaagtca gctcgctgcg gttaagcgta tagaagtgga aatctttcac gccttcgcgg
    14761 gacaggactt tgaccatatc gatggcgata cttgccgcca ccagattgcg cgtgccttgg
    14821 tcgtcgtcca aaccttcata catttgcgac agccagcttg ggattttgac gttggttacc
    14881 tgcgccattt tgccgagctg cttgaagttg gtaacgggca ggatgccggg gacgatttcc
    14941 acatcgatgc ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc cacatcgaag
    15001 aagaattggg tgatgacgtg gttcgcgccc gcatcgattt tgcgtttcaa attaatcagg
    15061 tcggcttgtg cggatttcgc ttcgggatgc acttcgggat atgccgctac agaaatgtcg
    15121 aagtcggcga cggagcgtaa tagcttgacc aagtcttcgg cgtaaaacgg ttttttctcg
    15181 taaccgggcg gctcgtcgcc acgcagggcg acaatgcggc ggatgccgct gtcccaataa
    15241 tctttggcaa tttggcgcaa ttcgtcggga gaagcgtcga taccggtcag gtgaggcgcg
    15301 gcttccaagc cggtttcctg tttgatgcgt ttgacgatgc cgtgcgtgcg gtcgcgttcg
    15361 ccggaatttg cgccgtaagt tacggatacg aacttgggat gcagggtttg cagacggtgg
    15421 atggagtccc acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa ttcaaacgaa
    15481 acgttaatgt cgcctttcaa atcagaaaga ctgttattta acgcattgat ttcttttgca
    15541 taattcatgg ctgcgtacct tttattatct tttatatgat attttcaggc aacataataa
    15601 agttacgcca aacatgagtc aatttcaaaa ttttcagatt tatcatgaaa atttctaata
    15661 tattacaaaa atagttaaat ttccttgtac aagccttcta atttatgata aagtttgtta
    15721 tgttataaca attcaactaa ggcgatatat gaaaccgaat attcatcctg acaattatcg
    15781 aaccgtcctt tttttcgaca gcagcgccaa tgaaggctgg ttaatccgct cctgtgccga
    15841 aacacacgga aaaacgatgg tttggacaga cggcaaagaa tatccgctgt tttcgctgga
    15901 tacttcttcc gcatcccatc ccgtctatac tggcaaacag cgcaacgtca acaccgaagg
    15961 ccgtgccagc aaattcaacc aacgtttcca atccgtcatg tcatcgttta gaaaggacaa
    16021 ataatgcagg ttctgtcttc actcaaaacc gccaaacaac gccaccgcga ctgccaaatc
    16081 gtcagacgca ggggcaaagt ttacgtcatc tgcaaaagca atccgcgttt taaagcacgc
    16141 caacgctgac cccctgccct tcctgcttcg gtt
//
LOCUS       DAWISC010000031        15784 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWISC010000031 DAWISC010000000
VERSION     DAWISC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.031"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(30..1073)
                     /gene="galE"
                     /locus_tag="WIW87_000690"
                     /pseudo
     CDS             complement(30..1073)
                     /gene="galE"
                     /locus_tag="WIW87_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1066..2068)
                     /gene="dcm"
                     /locus_tag="WIW87_000691"
                     /pseudo
     CDS             complement(1066..2068)
                     /gene="dcm"
                     /locus_tag="WIW87_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2099..4372)
                     /locus_tag="WIW87_000692"
     CDS             complement(2099..4372)
                     /locus_tag="WIW87_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ5173888.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSWLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4436..5086)
                     /locus_tag="WIW87_000693"
     CDS             complement(4436..5086)
                     /locus_tag="WIW87_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5173889.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KAFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5083..5880)
                     /locus_tag="WIW87_000694"
     CDS             complement(5083..5880)
                     /locus_tag="WIW87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5173890.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5880..7043)
                     /gene="ctrB"
                     /locus_tag="WIW87_000695"
     CDS             complement(5880..7043)
                     /gene="ctrB"
                     /locus_tag="WIW87_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ5173891.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7058..8233)
                     /gene="ctrA"
                     /locus_tag="WIW87_000696"
     CDS             complement(7058..8233)
                     /gene="ctrA"
                     /locus_tag="WIW87_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ5173892.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8360..9494
                     /gene="neuC"
                     /locus_tag="WIW87_000697"
                     /pseudo
     CDS             8360..9494
                     /gene="neuC"
                     /locus_tag="WIW87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
     gene            9498..10184
                     /gene="neuA"
                     /locus_tag="WIW87_000698"
     CDS             9498..10184
                     /gene="neuA"
                     /locus_tag="WIW87_000698"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ5173893.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10185..11234
                     /gene="siaC"
                     /locus_tag="WIW87_000699"
     CDS             10185..11234
                     /gene="siaC"
                     /locus_tag="WIW87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ5173894.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11318..12805
                     /locus_tag="WIW87_000700"
                     /pseudo
     CDS             11318..12805
                     /locus_tag="WIW87_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
     gene            complement(13301..14254)
                     /locus_tag="WIW87_000701"
     CDS             complement(13301..14254)
                     /locus_tag="WIW87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173895.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGCHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            complement(14274..14459)
                     /locus_tag="WIW87_000702"
     CDS             complement(14274..14459)
                     /locus_tag="WIW87_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173896.1"
                     /translation="MKKVLLIASYDSFLNSGYAVAKEIKDAQIDIYIHKSRENILSNR
                     QLLESGIDKDQAIFFSY"
     gene            14636..15655
                     /gene="galE"
                     /locus_tag="WIW87_000703"
     CDS             14636..15655
                     /gene="galE"
                     /locus_tag="WIW87_000703"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ5173897.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
BASE COUNT         4584 a         3623 c         3171 g         4406 t
ORIGIN      
        1 aggatggttt ttttgtttgc ggtttgcatt taatccccat atccgttggg gttgcggctg
       61 acccaacgcc acgaatcctc catcatttgc gcaaggctgc gtttggtttc ccagccagtc
      121 tcttgtttgg tatgggacgg gtcggcaaag gaacacgcca agtctccggc gcggcggggt
      181 tggatttgat agggaatatt taagccggaa gcggcttcaa aggcacggat gatttccaaa
      241 acggaatagg cgcgccccga acccaagttg aacaaatgta cgccggcaac gccgcctttc
      301 gccttcattg ccgcgatatg cccttctgcc aaatccatca catgaatata gtcgcgcatt
      361 cccgtaccat cgggggttgg ataatcgccg ccgaataccg acagttgcgg cagcctgccc
      421 gaagccactt ggcagatata gggcaaaagg ttgttgggaa taccgttggg ctgttcgccg
      481 atcaagccgc tttcgtgcgc gccgatcggg ttgaaatagc gcaacaaaat cacgctccag
      541 cgcggatcgg ctttttggat gtcggttaac atccgttcca ccatcgcttt ggacgcacca
      601 taaggattag cggtatcgcc cgggcgcata tcttccgtat agggcatttt acgtatcatg
      661 gcgcgacgca ttaaagtaaa cgccaaagca cgtttatact ggttatccag tttccctata
      721 tttttacgtt acaaatcaag ttgttggtat tcatcgtgaa aatagaattt ttcggcataa
      781 cgctgactca taaaaccggc atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca
      841 tttaatgttt catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa
      901 atatcgttag taatcacacg atagccgcgt tttttggctt cgtaggcaaa ggaacagcct
      961 ccactaaata catctgcaac tgtatctaca tcagacggaa gctggtcaca aatccaggaa
     1021 gctattttct ctttattacc gatatagtta attttcggat attgcttatt cattttcact
     1081 catttttaaa atactttcag caatagcttt tgccaataaa ggcggtacgg cgttaccaac
     1141 ctgctgctgc tgggcaattt tgctgccgca aaaaataaaa ttatcaggga aagattgtaa
     1201 ggcagctaat tcacgaacgg ttaacgcccg attctgttca tagtgaaaaa ctttgcgcat
     1261 atctcctgta atacaaacgg ctggttttgt tgctgttgta acggatgtat ttacggatat
     1321 cacctgtttt cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa
     1381 atgccatttt ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg
     1441 gattgctttc gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat
     1501 gggaaatctg caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata
     1561 tcactcggct acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca
     1621 ccgaataacc gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt
     1681 gtgtatagag tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta
     1741 ttcggacaaa ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct
     1801 ttcctgccat actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc
     1861 cgttaagaca attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt
     1921 gatgatgggg gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa
     1981 gcaattgttg gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa
     2041 ggtcaatata ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt
     2101 accgcttcag cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc
     2161 gttggggttt gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt
     2221 ttgcgccgcc cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat
     2281 cgacttccag cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt
     2341 ggacgaactt gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt
     2401 ccacgaacgc gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt
     2461 gcaagtcgct gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg
     2521 gatcacggcc gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc
     2581 gttcgtcggt gaagtcggac gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt
     2641 cggcggcgct aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt
     2701 ggacggcgct cgcgtccaac ggctctttac cgccgttaat ccgcaaaaag cctgccgcct
     2761 gctcgaaggt tttttcgccc aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg
     2821 cgccgttttc atcgcggtag gcaacgatgt tttgggcaag ggtttgattc aaaccggaaa
     2881 ttcgcgccaa gagcggggcg gaggcggtgt tcacgtccac gccgacggcg ttcacgcagt
     2941 cttcgaccac cgcgtccagc gatttggcga gccagctttg gttcacgtcg tgctgatact
     3001 gccccacgcc gatggatttg gggtcgattt tgaccaactc ggcaagcggg tcttgtagcc
     3061 tgcgggcgat ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg
     3121 ccagttcgga cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc
     3181 ccattttcga catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg
     3241 tgccgttacc gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg
     3301 ccaacatatt gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt
     3361 cgtccaccac ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca
     3421 gccgtccggc gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg
     3481 cgtcggtgtc ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga
     3541 ttttcgcgcg ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt
     3601 tgaagcggcg ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg
     3661 gctgatattt gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgccagtg
     3721 cacgatggct gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt
     3781 cgccttcggt ttctttgcct ccaacaactt gcgcgtggat ttcggcttcg ttccacagct
     3841 tgtcgcgcag cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac
     3901 gcgcgccgtc caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg
     3961 cggcttccac gtcctgcgac tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt
     4021 gttcgcgcgc gatttgcgct ttggtgcggc gtttgggttt gtagggcaga tacaggtctt
     4081 ccagcgcggt tttgttatcg gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc
     4141 cttgctcttc aatgcttttt aaaacaacgg ctttgcgctc ttccaactcg cgcagatatt
     4201 gcagccgctc ggcaagccgg cgcaactgcg tatcgtccaa cccgcccgtc gcttccttgc
     4261 ggtagcgggc gataaacggc acggtcgcgc cgtcgtccaa aagctcgacg gcggcggtga
     4321 tttgcgcggc agtcgcggag agttcttggg aaagaatttg agtaatgttc atcaatagaa
     4381 ttccaacgga caggccgtct gaaatttcag acggcctgat ttaaaaacaa tcgctttaag
     4441 gcagcgaatt ataatattcg taggctttgt ccatatcttc aaactggtac atatgccctt
     4501 tttccagcac catcgcatta tcgcaatatt gcttcatggc gctgtggctg tgcgacacca
     4561 agatgatgga acggtctttg cgcttttcaa acaactcgta cttacattta tcggcaaaac
     4621 gcgagtcacc aactgcaatc acttcgtcaa tcaggtaaca gtcaaactcc accgccaacg
     4681 acagcgcaaa agccaaacgc gctttcatac ctgaagaata gcgtttcacc ggctcataca
     4741 aatattgccc cagctccgaa aattcttccg taaacgcttt cacataatcg atatcgacat
     4801 tgtaaatccg gcagatgaaa cgcaaattgt ccataccggt cagactgcct tgaaacgcac
     4861 cggagaatgc caaaggccaa gaaatactca ttgtccgctt gatttcaccc gtggtcggcg
     4921 gctcaacgcc actgatcaaa cggatgagcg tcgatttacc tgcaccgttg cggccgagaa
     4981 taccgatttt ctcgcccttc tccattttga agctaatatc gtgcaagact gtccgccaac
     5041 cttggcgggt cagatagcgt ttggaaacgt gttcaactga aatcattgcg gctcgactcc
     5101 tttactgaat ttactgacca tcgccaagcc aaacaacaac aacaccagat tgcacaatac
     5161 gatataccaa ggattttcat aggtaattac atcgctgcca aaatatccgg cacggaacat
     5221 ttctgtgcca tgcaccatcg gaatcattaa tgcatattct tgtaccttgg gcggcaaatt
     5281 atgcacaaaa aagaacgcac cggataacgg catcatcaca aaagtcaatg tgccccaaat
     5341 cttgccaaac ggctcgaaat taaaggcaat cgaacaaatc accaaaccca aaccaatcgc
     5401 aaaaaaagcc atcaaaagcc aagccatcag catataaaac atatctgccg gcatttcaat
     5461 ccagccaatc gcaatcaata ccgccataat cacaatctgc gcaatggttg caccagcaat
     5521 ttccaaaatc atgcgcgcca agatggtatc caaaactctt acattgcggt gataaagcaa
     5581 gctggcattt gaagaaatcg acccaactgc ccgttttgag gcattacgcc acatcatcaa
     5641 catcggatag ccagtaatcg caaatgcgac aatattcaaa gttgaatatc ggtctgccct
     5701 taaaaatttc cacatcaaga cgataacgaa tgtcatcagc aacggctcaa caaacagcca
     5761 taaaaagcca atattattgc gaccgtaacg ggtgataatt tcccgcatca acagcgcacc
     5821 gattacgcgc ctttgaatgg ctaaagattc ccaaaatgat gttttatgca aggctttcat
     5881 cagtttttat gctcacgaat gctggcagtc aacaggctca aaataccata aaccatcaag
     5941 ccgataatca gagtggcaac aatgttgtat aaccgtttag gctcatgtgc caaatccggc
     6001 aggctcggtt gcgagatcac ttccaaataa agctgctgac ggtctgcttc aaccttggca
     6061 ctttccaaag aagtcatggc ggctgccaac tgctgctctg ccaactggtt ttccaaatac
     6121 acacgctgat attcggcagc ctgattagac aacgaagaat gcccaccgcc cgaaatggca
     6181 cgtaactgtt ggtcaatttc tttacgcaag ctctgctcac gcgcctgcaa acccggaatc
     6241 tgcggattct ccggagtgac tgctttcacc tgatccagct gggtttgaat cacaatcaat
     6301 tcatcttgca gcttggaaac caaccccatt tgcacttccg attgcgcttt caaatcaaaa
     6361 acgccattgg caatccggta atccgtcaga ttctgagagg cttcctttac ccgctctgcc
     6421 gccgttttca ctacttcttc cgcatagcgc accgtatcag cacgtgcacg atcgttcaac
     6481 tggttaatca atgcttcacc ttgttttaac aaagcctcat tgattttctt agattccagc
     6541 gcatcaaagg aagttacatt caacgtggaa atacccgaaa ccgtatcaaa attgatcatc
     6601 acctgatttt tatagtattg ataaaaagcc tcttcctcgc cacggaaccc aaacccatta
     6661 aagcggctga acgcatcacc tttggtttca taaaactcac gcaccggcaa gattttacgc
     6721 agttcatcca aagacgagcg cgaacgcata tactccccaa ccgtgtaaat atcatcttgc
     6781 gcacgggcaa aacctgtgcc ctgcaaaatg gcacccaggc cattgagaga agattggctt
     6841 ttaggcgagc gcaccacaaa gctcgattgc gacgtaaaac gatcggaagc gaagaagccg
     6901 aaatacacca acgaaattac cgtagggata atcaccgtta cccaaaataa agggcttagc
     6961 tttttaatcc aacttttctt tttcggcttt ttacgctcgg ctttggtttc agtggcaact
     7021 gccacaggaa gttgctcaga catcttaatt actcacatta attagttaaa ttattaatac
     7081 tgttcgcgcc actggtaacc ggcgagaaca caaacgacaa gaatttctgc acttcagcca
     7141 acggcgcatt cgacacatac aatacatctt tattcttcac aggaaagcgc tgcatagaaa
     7201 atagcgaatg cgcatcagcc atattcacac gatataccgt tggaatctct gcctcactgc
     7261 cataaccttg agcaatccat ttatcctgac gttctgccgg caattccacc aatggcgtat
     7321 agcggaacac aaacacacca cgcgcatcag aacggcgatc ttgcaaaccg cccatacggc
     7381 caatggcttc agaaagcgat aagcctctgg ctgaaaaacc gatttcttgt gttctcccca
     7441 ccgcacccat agacgtaaag gtatagggat tggtaatcat ggtaaccaca tcaccgcgac
     7501 gcagcaaaat attttgtcgc ggatttgcaa ctaaatcttc caaggcaaca gttcgtacta
     7561 cattgccacg tgtcagctgc acattcgtat cctgcacatt tgccgttgaa ccacctaccg
     7621 cagccaccgc atccaacaca cgctcaccgg ctgccgtcag cggcatacgc acactattcc
     7681 cagcacgaat caccgacaca ttcgccgcat tattctgcac caaacgcacc atcacttgtg
     7741 gctgattggc catttttttc aggcggcctt taataatttc ctgaacctga ccaggcgttt
     7801 taccgaccac cgaaatatcg ccaacaaacg gcacagaaac cgtaccacgt gccgtgacca
     7861 actgctctgg caacttagtt tgatgcgcac tacccgagcc catcgaagaa aggccaccac
     7921 caaacaatac tgccggcggc gcttcccaaa tcataatatc caatacatca ccaatattta
     7981 gcgtaccagc cgaagcataa ccatcgccaa actgagtgaa tgactgattt atctgagcct
     8041 tatataataa ctgagcaacc gtatgattca catcaatcag ctccacttca ggaatttgaa
     8101 cttcagattg ttgccctaaa gagacaattt tttttgcgct ggggcctgat gaaggaatcg
     8161 cagagcatcc tacaattaaa cttccacaca ataataatac tgcgtgacga atataaaatt
     8221 tcactttaaa cacaagccaa atcctaatat aattataaat ggcctaatta tagcacttaa
     8281 tcgaaataaa tttatgagta cgtagagtat aattagtatt cttctttcca acttccttat
     8341 gcctatagat tctaaaatca tgaaaagaat tctttgcatt acaggtacca gagccgactt
     8401 cggcaagcta aaacctttat tagcctatat tgaaaatcac ccagaccttg aattgcattt
     8461 gattgtaact ggtatgcata tgatgaaaac atatggcaga acctacaagg aagtaactcg
     8521 agaaaactat caacatacat atctgttttc aaatcaaatc caaggtgaac caatgggtgc
     8581 cgttttaggc aataccatta cgtttatctc tcgtctatct gatgaaattg aacctgatat
     8641 ggtcatgatt cacggcgacc gtttagaagc actagcaggc gcaactacta ggtgcattaa
     8701 gcagccgttt agtttgccat atcgaaggtg gtgaactatc tggtacagta gatgactcca
     8761 ttcgtcattc tattagtaaa ctttctcata tccacttggt agcaaatgaa caagctgtca
     8821 ctcgcctagt gcaaatggga gaaaaaagaa agcatattca catcatcggc tcccccgatt
     8881 tagatgttat ggcctcttcc accctcccat ccttagaaga agtcaaagaa tattacggtt
     8941 taccatacga aaattatggt atttctatgt ttcaccccgt gactacagaa gcacatttaa
     9001 tgccacaata tgcggcccaa tatttcaaag cattagaatt aagtggccaa aatatcatta
     9061 gcatctaccc taataatgac actggcactg aaagtattct gcaagaatta ttaaaatatc
     9121 aatctgataa atttattgca tttccttcca ttcgatttga atatttttta gtcttattga
     9181 aacatgctaa atttatggtc ggcaactcaa gtgcaggtat tagagaagct cctctctacg
     9241 gtgtcccttc aattgatgtt ggtacacgcc aaagtaaccg ccatatggga aaatctatta
     9301 ttcatacaga ttatgaaact aaaaatatct ttgatgcgat tcaacaagca tgcagtttag
     9361 gcaaatttga agcagatgat acctttaatg gcggagatac tcgcaccagc acagaaagat
     9421 ttgctgaagt aatcaacaat cctgaaacgt ggaatgtttc tgctcaaaaa cgttttatcg
     9481 atttgaatct ttaaattatg gaaaaacaaa atattgcggt tatacttgcg cgccaaaact
     9541 ccaaaggatt gccattaaaa aatctccgga aaatgaatgg catatcatta cttggtcata
     9601 caattaatgc tgctatatca tcaaagtgtt ttgaccgcat aattgtttcg actgatggcg
     9661 ggttaattgc agaagaagct aaaaatttcg gtgtcgaagt cgtcctacgc cctgcagagc
     9721 tggcctccga tacagccagc tctatttcag gtgtaataca tgctttagaa acaattggca
     9781 gtaattccgg cacagtaacc ctattacaac caaccagtcc attacgcaca ggggctcata
     9841 ttcgtgaagc tttttctcta tttgatgaga aaataaaagg atccgttgtc tctgcatgcc
     9901 caatggagca tcatccacta aaaaccctgc ttcaaatcaa taatggcgaa tatgccccca
     9961 tgcgccatct aagcgatttg gagcagcctc gccaacaatt acctcaggca tttaggccta
    10021 atggtgcaat ttacattaat gatactgctt cactaattgc aaataattgt ttttttatcg
    10081 ccccaaccaa actttatatt atgtctcatc aagactctat cgatattgat actgagcttg
    10141 atttacaaca ggcagaaaac attcttaatc acaaggaaag ctaaatgcaa aacaacaacg
    10201 aatttaaaat tggtaatcgt tcagtaggtt acaaccacga accattgatt atctgtgaaa
    10261 tcggcatcaa tcatgaaggc tctttaaaaa cagcttttga aatggttgat gctgcctata
    10321 atgcaggcgc tgaagttgtt aaacatcaaa cacacatcgt tgaagacgaa atgtctgatg
    10381 aggccaaaca agtcattcca ggcaatgcag atgtctctat ttatgaaatt atggaacgtt
    10441 gcgccctgaa tgaagaagat gagattaaat taaaagaata cgtagagagt aagggtatga
    10501 tttttatcag tactcctttc tctcgtgcag ctgctttacg attacaacgt atggatattc
    10561 cagcatataa aatcggctct ggcgaatgta ataactaccc attaattaaa ctggtggcct
    10621 cttttggtaa gcctattatt ctctctaccg gcatgaattc tattgaaagc atcaaaaagt
    10681 cggtagaaat tattcgagaa gcaggggtac cttatgcttt gcttcactgt accaacatct
    10741 acccaacccc ttacgaagat gttcgattgg gtggtatgaa cgatttatct gaagcctttc
    10801 cagacgcaat cattggcctg tctgaccata ccttagataa ctatgcttgc ttaggagcag
    10861 tagctttagg cggttcgatt ttagagcgtc actttactga ccgcatggat cgcccaggtc
    10921 cggatattgt atgctctatg aatccggata cttttaaaga gctcaagcaa ggcgctcatg
    10981 ctttaaaatt ggcacgcggc ggcaaaaaag acacgattat cgcgggagaa aagccaacta
    11041 aagatttcgc ctttgcatct gtcgtagcag ataaagacat taaaaaagga gaactgttgt
    11101 ccggagataa cctatgggtt aaacgcccag gcaatggaga cttcagcgtc aacgaatatg
    11161 aaacattatt tggtaaggtc gctgcttgca atattcgcaa aggtgctcaa atcaaaaaaa
    11221 ctgatattga ataatgctta ttaacttagt tactttatta acagaggatt ggctattaca
    11281 tatagctaat tctcattaat ttttaagaga tacaataatg ctaaagaaaa taaaaaaagc
    11341 tctttttcag cctaaaaagt tttttcaaga ttcaatgtgg ttgacaacat ctccatttta
    11401 tcttaccccc cacgtaacaa tttatttgtc atatctaatt taggtcagct taaccaagtc
    11461 caaagcctaa ttaaaataca aaaattaacc aataatttac tagtaatttt atatacttct
    11521 aaaaacttaa aaatgcctaa gttagttcat caatcagcta acaagaatct atttgaatct
    11581 atttatctat ttgagcttcc tagaagccct aataatataa ctcctaaaaa attactttat
    11641 atttatagaa gttacaaaaa aatccttaat attatacagc ctgctcatct ctatatgctg
    11701 tcttttacag gccactactc ctatctgatt agtattgcaa aaaaaaaaga atattacgac
    11761 tcatttaatt gatgaaggga ctggaacata tgctccttta ttagaatcat tttcatatca
    11821 tccaacaaaa ttagaacgtt atttgattgg aaataatctt aatattaaag gatatataga
    11881 tcattttgac atattgcatg tcccctttcc tgaatatgct aaaaaaatat ttaatgcaaa
    11941 aaaatataac cggttttttg cgcatgctgg aggaataagc attaataata acattgcaaa
    12001 cttacagaaa aaatatcaaa tatctaaaaa tgactatatt tttgttagtc aacgctaccc
    12061 catttcagat gatttgtatt ataagagtat agtagaaatc ttaaacagca taagtttaca
    12121 aattaaagga aagatattta ttaaactaca cccaaaagag atgggcaaca actatgtaat
    12181 gtctttattt ctaaatatgg tagaaataaa ccctcggctg gtagttatta atgaacctcc
    12241 ttttctaatt gagcccctaa tatacttaac aaatcctaaa ggaattatag gcctggcctc
    12301 tagttcttta atttatacac cattactctc accctcaacc caatgtcttt ctattggaga
    12361 gttaattatt aacttaattc aaaaatattc aatggtggaa aacactgaaa tgatccaaga
    12421 acacttagag attattaaga aatttaattt tattaatata ctaaatgatt taaatggggt
    12481 aataagtaac cctctcttta aaacagaaga aacatttgaa acacttctta aatctgcaga
    12541 attcgcatat aaatctaaaa actactttca ggctattttt tactggcaac ttgccagcaa
    12601 aaacaatatt accttattag ggcataaagc attatggtac tacaatgcac tttataatgt
    12661 aaaacaaatt tataagatgg aatattcaga tattttttat atcgataata tctccgtaga
    12721 ctttcatagt aaagataaat tgacatggga aaaaattaaa cattattact attccgccga
    12781 caatagaatt ggtagagata gataaaaata ctatgacttc tgatctccat acgtatgctt
    12841 gttttcaaaa agcagcatta agatagataa gtgaagcttg atgagagcca tttttgtggt
    12901 catcgccaag gtacacgcgg agagtaatcg acaacagagc tgctgtattc tggcttttaa
    12961 agcccagtag taaagtttat aaggctgcat ttctcaatgt aaaacgcgat attttcaccc
    13021 attattaggg ataagtagcc agtggtttac actggctact ttcatagtta tgaagtactg
    13081 ggtctgagtg aattcaatta tttccgtata aaacacagta gacattttac taatcggcaa
    13141 aattatatta gaagaattga gaactactga agtcaggtaa gataccattt tgcacaaatt
    13201 taacggcatc cctaaaaagc attttgggtg gtatttaaag gagtacaaaa tggttttaac
    13261 tacagtatgc ttagaaatct atagccttac gcgctttata ttaaatcacc tttctcaacc
    13321 atttagaatc ataaaaaaac tttttaggat ccctaattaa ctttttaaat tttcttaaaa
    13381 aaatagtttt agttgattct atactattca ctgcagataa atattcttgg taatcatgtt
    13441 gcaatggcac aacctgcttt aatagcttat taaactcatc agttgtaatc acctttttag
    13501 tagcatagca atcaagccaa tccggatctg ctatttttgg ggaagtgaaa tctatttgat
    13561 caaaataaac taaaacccca ctacctaaaa aatgctgatt tgcataggat ttagaaaagc
    13621 ctaagtctgc aacaacacca acagggatac ccttacattc agcttcaaaa agcatagtag
    13681 atgaataaga taaacaatac cccatttctt ggaaggcttg cgcggtactt tttcgctcta
    13741 ttgtcaaatt actcggcaac tgaaactgct ttgccagctc tatatacgaa tgtttatcct
    13801 gatgcacagt aatatcttta tctgcaaccc ttagcaaaat agtaaattct ttctcagggt
    13861 atttttcagc caaggcaatc aattttttta atgtataaat tctttcttct tttttaaatg
    13921 ggattttaac ttggtcaata aagtaaattt tctctccatg acaacctttc gaagcatggc
    13981 gaaaaattgg ataaccataa agtatatttt gacaacttaa tttatattgt ttcttatatt
    14041 cctctgcaat tctaaaatca tgcttattat tatataaaac aatatcagcc cccatacgag
    14101 atagaatact tgcctgatca ccgaatacta cacctggaaa caaggtaata atcaatggcc
    14161 ttgaagcaat atttaattgc gcattctgct taaagaacct ttttaacaac ccatttccaa
    14221 ccgataaaat tactgcgtca taatattgat gcatattctt aataaagtaa tcatcaatat
    14281 gaaaaaaaaa ttgcttggtc tttatctatc cctgattcta ataactgtcg atttgaaaga
    14341 atgttttctc gacttttgtg gatataaata tcaatttgag catcttttat ctcttttgca
    14401 acagcatagc ccgagttaag gaacgagtca taactggcaa ttagtaatac ttttttcact
    14461 attttccctt aattctacac catatgaata aaattatagt attttacctt agatatcaaa
    14521 tatacttaac caaaaaaatc tgaagaaatc tactgctact tcagggataa ttatttcatt
    14581 aagattttac aaccattatt atcaatctat caaagtattt ttagtatttt attctatgaa
    14641 aaaaattctc gttaccggcg gcaccggttt tatcggctcg cataccgttg tttctttgct
    14701 gaaaagcggc catcaagtcg tgattttgga taacctatgc aattccagca tcaatatcct
    14761 gccacgcttg aaaacgatta ccggccaaga gattccgttt tatcaaggcg atatccgcga
    14821 tcgtgagatt ttgcgccgta tttttgcgga aaaccgcatt gattcggtga ttcattttgc
    14881 cggcttgaaa gcggtgggtg aaagtgtggc cgagccgatg aaatattatg ataataatgt
    14941 ttccggcagc ttggtgttgg cggaagaaat ggcgcgtgcg ggcgtgttta gcattgtgtt
    15001 cagttcttcg gcgacggttt atggcgatcc gggcaaagtg ccttataccg aggatatgcc
    15061 accgggcgac accaccagcc cttacggcgc atcgaaatcg atggttgagc gcattctcac
    15121 tgacattcaa aaagccgatc cgcgctggag catgattttg ttgcgttatt tcaatccgat
    15181 tggcgcgcat gaaagcggct tgattggcga gcagccaaac ggcatcccga ataatttgtt
    15241 gccttatatc tgccaagtgg cggcaggcaa actgccgcaa ttggcggtat ttggcgatga
    15301 ctaccctacc cccgacggca cggggatgcg tgactatatt catgtgatgg atttggcaga
    15361 aggccatgtc gcggctatgc aggcaaaaag taatgtagca ggcacgcatt tgctgaactt
    15421 aggctccggc cgcgcttctt cggtgttgga aatcatccgc gcatttgaag cagcttcggg
    15481 tttgacgatt ccgtatgaag tcaaaccgcg ccgtgccggt gatttggcgt gcttctatgc
    15541 cgacccttcc tatacaaagg cgcaaatcgg ctggcaaacc cagcgtgatt taacccaaat
    15601 gatggaagac tcatggcgct gggtgagtaa taatccgaat ggctacgacg attaagttga
    15661 cctgatacag gccgtctgaa agagatgttt tcagacggcc tctttatctg aaaaacacac
    15721 attctgtctg ctataatctg tttatatttt ttggctatcc tctgaaattt atgagaaaaa
    15781 tcct
//
LOCUS       DAWISC010000032        15406 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWISC010000032 DAWISC010000000
VERSION     DAWISC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.032"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WIW87_000704"
     CDS             complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WIW87_000704"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5173898.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1938..2375)
                     /locus_tag="WIW87_000705"
     CDS             complement(1938..2375)
                     /locus_tag="WIW87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173899.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WIW87_000706"
     CDS             complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WIW87_000706"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ5173900.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WIW87_000707"
     CDS             complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WIW87_000707"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ5173901.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7583..9295
                     /locus_tag="WIW87_000708"
     CDS             7583..9295
                     /locus_tag="WIW87_000708"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ5173902.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYQDMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAELVKVHTPNVKTIDSLVDFLSIPIEK
                     TLKSIVVEGENEGELILLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            9358..10014
                     /locus_tag="WIW87_000709"
     CDS             9358..10014
                     /locus_tag="WIW87_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ5173903.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WIW87_000710"
     CDS             complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WIW87_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ5173904.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10472..11020)
                     /locus_tag="WIW87_000711"
     CDS             complement(10472..11020)
                     /locus_tag="WIW87_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ5173905.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(11022..11582)
                     /locus_tag="WIW87_000712"
     CDS             complement(11022..11582)
                     /locus_tag="WIW87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ5173906.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11910..13700
                     /locus_tag="WIW87_000713"
     CDS             11910..13700
                     /locus_tag="WIW87_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ5173907.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDIWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13827..15311
                     /locus_tag="WIW87_000714"
     CDS             13827..15311
                     /locus_tag="WIW87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ5173908.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3698 a         3794 c         4251 g         3663 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata ggtcggagaa gatattttaa tccgctgtaa
       61 aatagaaaat gccgtctgaa catttcagac ggcatttgct caaataaacc ttatcggttg
      121 tttattcagt cggatttatt ttttcttttg cggaggcagg tcggtacaag tacccaatgc
      181 cacttccgcc gccataccga tggattcgcc caaggtcggg tgcgggtgga tggttttgcc
      241 gatgtctgcc gcgtcgcagc ccatttcgat ggcaaggcag acttcgccga tcatatcgcc
      301 accgttcgga ccgacaatgc cgccgccgat gatgcggccg gtttcggcat caaaaatcag
      361 cttggtaaag ccgttgtcgc aaccgttggc aatcgcacgg ccggaagccg cccacgggaa
      421 gttggctttg gtgattttgc ggccggaggc tttggcggac agttcggttt cgcccaccca
      481 cgccacttcg ggggaagtgt aggcaacgcc cggaatcacg cgcgcgtcga agtaggcttt
      541 gtggccggcg cagttttcgg cggcaacgtg gccttcgtga acggctttgt gcgccaacat
      601 cggctgaccg acgatgtcgc cgatggcgta gatgtgcggc acattggtac gcatttgttt
      661 gtccacttcg atgaagccgc gatcggttac ggcaacgcct gctttttccg cgctgatgag
      721 tttgccgttg ggcgcgcggc cggcggcaac caatacggca tcgtagcgtt gcggctcttt
      781 aggcgcgttc gcgccttcaa aggtaacgta aacgccgtct tctttcggct caactgcaac
      841 ggttttggtg ttgaccataa tgttgtcaaa acggtattcg ttttgttttt gccatacttt
      901 taccaaatcg cggtctgcgc cttgcatcag gccgtccatc atttcaacca catccaaacg
      961 cgaacccagc gtgctgtaaa ccgtacccat ctcgaggccg ataatgccgc cgccgataat
     1021 cagcagtttg cccggtactt ctttcagagc caatgcgccg ctggaatcga tgatgcgcgg
     1081 atcttcagga atgaaaggca gtttggttac gcggctgcct gctgcaatga tacagttttt
     1141 gaaggcaacg atttttttct cgccggtagg ggctgcctgt tcgtacgcgt cgccggcagt
     1201 cagcgacact tccaagtggt gcggatctaa gaattgcccg tcgccttgga taacgtccac
     1261 tttacggctt ttcgccatac ctgccaaacc gcccgtgagg cgggaaacta cgccgtcttt
     1321 gtaggcgcga agcatatcga tgtcgagttc cggctcgggg tatttgatac cgttggcagc
     1381 caagtggcgc acttcgtcga taacggcggc attgtgcaac aaggctttgg aagggataca
     1441 gccgacgttc aggcaaacgc cgcccaaagt tttgtaacgt tcgacgatgg cgactttcaa
     1501 gccttcatcg gcagcggcaa atgcagcgga gtaaccgccg ggaccgccac ccaatacgac
     1561 cacgtcgtac tcggcatcgg cagaaccgcc gaattgcgcg gcttgcggag caggagcggc
     1621 agctttaggg gcttcttgcg ccggggcggc agccgcttcg gctttaggag cggctgccgt
     1681 gccttcagct tcaacgacga caatcaaacc accttcagag attttgtcgc cgactttaac
     1741 tttaacttct ttgactacgc ctgcaacttc agcaggtacg tccatagtcg ctttatcggt
     1801 ttccaaagta atcagggtat cgtccacagc aatagtgtcg cccacgttta cttcaaccgc
     1861 gataatatct acattttcgt gtccgccaat gtcgggcact ttcaattcaa ctaaagccat
     1921 tatattgacc tttcaactta gttaatttct atcaactctt tagcaataat cacttcttca
     1981 ggaatacagc cacaatcttc agctctgatt gttacttcct tctcatttgg gttttctgga
     2041 tgttcaacct catattcaaa aatagaatat tgaccaaaca aatccctatt taaaacatat
     2101 atatagccat tttcgatgcc ggaacttgtt gcaaatttct tggcaatttc cttgtctgtt
     2161 gtcgtagata tataacatcc gtcatataga cctgtttcaa tttgatgggc gtaaactgca
     2221 ttcttcacac ttggaccatg tgtagcttta ccatcatatt taaacttacc atcataacga
     2281 attgcaactt cagctttatt acctttaggt tttaactgtc cattattttg ctcatcttgt
     2341 tggcaactaa tgcctctata taagaaattt cccataagtt attaatttaa aggttgaatt
     2401 taacaccatt actcatcaat ctcaagcaat ccaaacatta tcagactgct tgagagggat
     2461 gttttatttt ataaggtaat gcggcggaag tctttcaaca gcttcgccaa gaatacggtg
     2521 aagcgcatac cggccgcacc gtcgatgaca cggtggtcga aggacaggct caacgggcac
     2581 atcaggcgcg gggcaaactc tttgccgttc caaacaggtt tgatttggga tttgcacacg
     2641 cccaagatgg cgacttcggg agcgttcaca attggcgtga agcctgtgcc gccgatgccg
     2701 cctaagctgg aaatggtaaa gcacgcgcct tgcatttctt gcggtttgag cttgccttca
     2761 cgggcttttt tggacaattc ggtcaattct tggctgattt gtttcaagcc tttttgatcc
     2821 acgtctttga tgacgggaac aaccaagccg ttcggcgtat cggctgcgaa accgatgttg
     2881 aagtagtttt tcagcaccag gttgtcgccg tccagtgagg cgttgaattc ggggaatgct
     2941 ttcaacgcgg aaacagaggc tttgatgatg aacgccaacg gggacagttt cacgccttcg
     3001 cgttcccatt ctttgttcag ctgtttgcgg aattcttcca gctcggtcat gtccgcttct
     3061 tcgtgtacgg taacgtgggg aatcacaacc cagttgcggg acaggttttg accggaaatt
     3121 ttcttaatgc gggacaattc tttaacttcg acattgccga atttggagaa gtccacttta
     3181 ggccacggca gtaagtccag accgccgccc aaagatgcgc tggctgcggc aggttttgcc
     3241 gcgccgccct gcatcacgga tttcacaaag gctttgatgt cgtcgcccat gatacggcct
     3301 ttcaagccgg tgcctttgac ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt
     3361 gcggaaggac cggcgtgtgc tttggcgaaa gcggcctcgt cgattttggc agcggcaggt
     3421 gcagaaggtg caggcgcggc ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc
     3481 ggtgcggcag cttgagcggc ttgagcaggg gctgctgcgg cagagccgac ggtttctact
     3541 tcgataatgg cagagccttc ggatactttg tcgccgactt ttaagaatac ggctttaacg
     3601 acaccggcag cggtacaagg tacgtccatt gtcgctttat cggtttccaa agtaatcagc
     3661 gtgtcgtctt cggcaacggt gtcgcccact ttgatttcaa cggcgattac atccacatcg
     3721 gtatggccgc cgatatcggg aacggctact tgaacggttg caccgcctgc ggcagcgggt
     3781 gcggcagcag gtgcaggttg tgcttcggca gcagccgggg cggcttcggc ggcggcggca
     3841 ccggtttcaa cggtcagaat tacgccgcct tcggagattt tgtcgccgac tttgactttt
     3901 acttctttca cgacaccggc cgcatcggca ggcacatcca tcgtggcttt gtcggtttcc
     3961 agtgtaatca gggtgtcgtc aacggcgatg gtgtcgcccg ctttaacttc tacggcgatg
     4021 atgtcgacgt tttcgtgacc gccgatatcg gggactttga tttctacgat actcatttga
     4081 gttcctttaa tctttttcaa ttggatgccg tctgaaagct gcttcaggcg gcatcggtac
     4141 ggtttgtctg ctcaaaccat tcgggcattc ggttttcaga cggcctgatg tcggaatggg
     4201 gcaaacaggc cgtctgaaac atcaatcagc gtttccagct aggagctgaa tcggctttga
     4261 tgccgtattt ctcaatggct tgttgaacgg tttctttgct gactttgcct tgttccgcca
     4321 atgcggccaa tgcggccacg gcaacgttgt agcgatccac ttcaaagaag cggcgcaggt
     4381 tggcgcgact gtcggaacgg ccgaaaccgt cagtgcccaa gacgtggtag tcgttcggga
     4441 tgtaggcgcg gatacggtca gcatagctgc ggatatagtc ggtagcggca ataaccggac
     4501 cgtcatgacc ttgcagttgg gaagtaacga aaggtacttt ttcggcttcc agcggatgca
     4561 ggcggttgaa gcgttctacc tcgacagcgt cgcggtgcag caggttgaag gacgggcaag
     4621 accagatgtc tgcttctacg ccgaagtcgg ctttcagcag ctcggcaccg gcaatgactt
     4681 cttgcaggat ggtaccggag cccatcaatt gaactttctt atcgcctttg ccgccggctt
     4741 tcagcaggta catacctttc aagatgtctt gttccgcacc ttcgggcata tccggatggg
     4801 tgtagttctc gttcatcagg gtgatgtagt agaacacgtc ttcattattg gcatacatac
     4861 ggcgcagacc gtcttgtacg atgacggcga cttcgtattg gaaagtcggg tcataagata
     4921 cgcagttcgg aatcaggtcg gcctggatgt ggctgtggcc gtcttcgtgt tgcaggcctt
     4981 cgccgttcag cgtcgtacgg ccggcagtac cgcccagcag gaagccgcgc gcgtgcatat
     5041 cgcccgccgc ccaagccaag tcgccgatac gttggaaacc gaacatagaa tagtaaatgt
     5101 agaacggaat catggcgaag ttgctgttgg cgtagctggt tgcagccgca atccagtcgg
     5161 ccatcgcgcc cggttcgtta ataccttctt gcaagatttg accgtcaacg gattctttat
     5221 agaacatcag ttggtctttg tcttgagggg tatattgctg acctttcgga ttccaaatac
     5281 cgtattggcg gaacatacct tccatgccga aagtacggct ttcgtcggga acgataggta
     5341 cgacgcgttt gccgattttt ttgtctttca gtaaagtgga caggatgcgg acgaatgcca
     5401 tcgtggtcga gaactcgcgt tcaccgctgg atttgagttg tgcgtcgaat gctgacagct
     5461 cgggcacttc caatacttcc tgcgtcggtt tgcgttgcgg caggtagccg cccaaagcat
     5521 cgcggcgtgc gtgcaggtat ttgtattctt ccgtatcggg ggcaaaagtc aggtaaggca
     5581 gatcgccgct ttcgatttgt tcgtcggtaa ccggaatgtc aaagcggtcg cggaattgtt
     5641 tcagggacgc tttgtccatt tttttggctt ggtgggcaac gttctgacct tcgccggatg
     5701 cgcccatacc gtaaccttta atggttttcg ccaagatgac ggtaggtttg ccgtcggcat
     5761 ggttcgctgc gcggtcgtag gcgttgtaca ctttttgcgg gtcgtggccg ccgcggttca
     5821 atgcccagag ttgctcatcg gtcatatcgg caaccaatgc tttcagttcg ggcgtattga
     5881 agaagtgttc gcgcacatac gcgccgtctt tggatttgta agtttggtag tcgccgtcca
     5941 aacattcttc catacgttgg cgcaggatac cgtctttgtc tttcgccaag aggcggtccc
     6001 agcggcggcc ccaaatgact ttgacgacat tccagccggc gccggcaaag ttgccttcca
     6061 attcttggat gattttgccg ttgccgcgta ccggaccgtc caagcgttgc agattgcagt
     6121 tgatgacgaa aatcaggttg tccaagcctt cgcgtgcagc cagtgcgatt gcaccttgag
     6181 attcgggttc gtccatttcg ccgtcgccgc agaaacacca tactttacgg cctttggttt
     6241 ttgccaaacc acgcgattcc aagtatttca ggaaacgcgc ctgataaatc gccatgatgg
     6301 gccccaagcc catggatacg gtcgggaact gccaaaagtc gggcaagagg tgggggtgcg
     6361 gataggaagg cagaccgtgt ccgtccactt cttggcggaa gttgttcagc tggtcttcgg
     6421 tcagacggcc ctcgacgaat gcgcgtgcat agatgcccgg ggcgacgtga ccttggaaga
     6481 agaccaaatc gccttcttcg ccttcgcctt tggctttcca aaagtggttg aaaccgactt
     6541 cgtacatggt ggcggcagat tggaaagatg cgatgtgccc acccagttcc aaatctttct
     6601 tgccggcgcg caatacgatg gcggcggcgt tccagcgcac gaatgcgcgg atgcggtgtt
     6661 cgatgttttg gtcgcccgga atgccttttt cgttttcaac cgaaacggta ttcaaatacg
     6721 gggtggtcgt gccgtgtggc atacgtacgc ccttgtcgcg gcagtatttg accaggtttt
     6781 ccaagaggta ttgcgcgcgt tcgccgcctt catattcgag gacggagctt aacgcgtcca
     6841 gccactcttg ggtttcgata gggtcaacat cgtgtaattg ggtggacata gtatctatcc
     6901 tttatgttga gtgtttaatc ggcagggagg gtgtagccaa cccctttatt ttcgtttgtg
     6961 aaaaataata agcaaaaacg aaaatttcta aatcaaaccg aaaaaaataa tttaagttta
     7021 atgttttcaa aaaattataa aagttttgga aacaagggaa aacttgaaaa aaatgttcga
     7081 tctgctccac acgaaatcct atcagcaatc gggtctgctg gcaaataacc atgcctgtaa
     7141 aagcaggttt tatactgaag ttggcgtttg gtaaggtcaa gtctttttga tttcgaaata
     7201 ttctgtttga ttttggcgat attttttcgg aattgtggta tatcgaatca ttatgtctct
     7261 gtcggcaatt ttcctattgt agaaaatcta tacacaagct ttgtggataa ccgtgttgat
     7321 aagccacggc ttgtgttaaa aaagtcttac gcaacaaatg tacgcttatt ttgattaaaa
     7381 aataggcaca tttaaatcta taaaaatcat gtgcttatgt tcaatatgat gtttctggtc
     7441 atgtttttgc cgtctgttat gggatttgtc ccgcgcttgg ggcagggggt gaattttgga
     7501 ggttggtgca aaacctgctt ttttcgcgtt acaatctgca cccgatttac ttttgaattt
     7561 gcctgtttgg aaaaatactt ttatgaaagc cagccaattc tttatctcta ctttaaaaga
     7621 agcccctgcc gaagccgcgc ttgccagcca caagctgatg attcgcgccg gcctgattaa
     7681 ggccaacgca tcgggtcttt atacttggat gccgatgggg ctgcgcgtgt tacgcaaagt
     7741 cgaaaacgtc gtgcgcgagg aaatggcgcg cgcgggcagc gtggagttgc tgatgccggt
     7801 ggtgcagcct gccgagctgt ggcaggaatc cggccgctgg gagttttacg gtaaagaact
     7861 gctgcgcctg aaagaccgcc acgaccgcga tttctgcatg ggcccgacct gcgaggaagt
     7921 catcgccgac atcgtgcgca aagaaatcaa cagctacaaa cagctgccga aaaactttta
     7981 ccacatccaa accaaattcc gcgacgaagt gcgcccgcgc ttcggcgtga tgcgcgcgcg
     8041 cgagtttgtg atgaaagacg cttattcctt ccacgccgac tatgcctcgc tccagacgac
     8101 ctatcaagat atgtatgatg cttattgccg catctttacc cgactaggct tggcgttccg
     8161 acccgttgct gcggataccg gcagcatcgg cggtaccggt tcgcacgagt ttcaagtgtt
     8221 ggcggaaagc ggcgaagatg tgattgcata cagcgacact tccgattacg ccgccaatat
     8281 cgagttagcg ccgaccttgc cgctcaaagg cgaacgtgca gccgctcagg cagaactcgt
     8341 caaagtacac acgccaaacg tcaaaaccat tgattcattg gttgatttcc tcagtattcc
     8401 gattgaaaaa acactgaaat ccatcgtggt tgaaggcgaa aacgaaggcg aacttatcct
     8461 gctgctgtta cgcggcgacc atgaatttaa cgacatcaaa gccgagaaac tcgcgggcgt
     8521 gaaatcgccg ctgacgatgg caagccctgc cgcgattgtc gaacagttcg gcgcaaacgg
     8581 cggctctctc ggccctgtcg gttttgcagg caaagtctat gccgatttcg ctaccgaaaa
     8641 aggcgcggac tgggtcatcg gcgcaaacga agacgactac cactataccg gcttcaattt
     8701 cggccgcgac gctgccgaac ctgagtttgt cgatttgcgc aacgtcgtcg agggcgacga
     8761 aagccccgac gggcaaggcc gtctgaaact ggcgcgcggc attgaagtcg gacacgtttt
     8821 ccaattgcgc gacaaataca cccaagccat gaacgtaagc ttccttgaca acaacggcaa
     8881 atcgcaaatt atggaaatgg gctgctacgg catcggcatc acccgcgtcg ttgccgccgc
     8941 catcgagcag aataacgacg aaaaaggcat catctggacc aaagcaatgg cgccgtttga
     9001 agtcgttatc gtgccgatga actacaaaaa atcagacacc gtacgcgaag ccgccgacaa
     9061 aatctatgcc gaattgctgg cggcaggcgc agacgtcctc ctcgacgacc gcgacgaacg
     9121 cgcaggcgtg ttgctgaacg attccgagct gttgggtatt ccgcaccgca tcgtcatcgg
     9181 cgaccgcgcc ttgaaagaag gcaatgtcga atacgccgaa cgccgcgaca acgaagcgca
     9241 ggcagttgca atcggagaaa ttgttgcacg tgtaacagct tcattaaatg cgtaagcttg
     9301 gtcgaatgcc gtctgaaaat aatttcagac ggcattttta cttaggggga aagtgaagtg
     9361 gcttcagtat ttttagaagg agaggcagtc gaggtcggca atattttctg tatcggcagg
     9421 aactatgccg cacatatcga agagttaaaa aacgaaatac cttccgaacc tgtggtgttt
     9481 atgaagccgt caggcagcat tttgaacagc ggcggtacaa tcttgctgcc cgaatttagt
     9541 agggatgtgc agtttgagtg cgaactcgtg ctgctggtcg gtaaggattc agacggcacg
     9601 ggcgagggta aagatatttt gggatgtgtc gcaggatatg gtgtagggct ggaccttacg
     9661 gcacgggata tccaatgccg tctgaaggaa aaagggctgc cctggctgaa ggcaaaaggg
     9721 ttcaggcatt ccgcgtgcgt gtcggatttt gcggcggcag gcagaatcgg caatcccgag
     9781 aaggtgttgt tttcattaaa acaaaacggc gtattgaaac agcgcgggga taccggtttg
     9841 atgatttacc cgattcggga aattttacac aaactggcgg cagattacgg tttggggaaa
     9901 ggggatttgg tgtttaccgg aacgccctcc ggcgtgggcg caatcggtgc gggggacaat
     9961 ttggcattgg agctggacgg cttggtgcgc gcctctttca cggtagggtg ttaaccgcgc
    10021 cgcttaaccc tcacgcccgt tgaaacattc cgccggaccg ccctcgaaaa aaccgtgcaa
    10081 gatggcttgc gccgccactt ggtcgagcac cgatttgcgt tttttgccga agacctgtgc
    10141 ttccgaaagc aggctttcgg catagacgga cgacagccgt tcgtcaaccc aatagacggg
    10201 gagattgaac ctgccgttca gcctgcgtcc gaacttgcgc gacaggtgcg tcatttcatg
    10261 tttcgtgccg tcggtatgca cgggcagtcc gacgacaaaa taacgcggct gccattcttg
    10321 aaccagcttg gcgattgccg cgaacttttc atcgttgctg ccgccggtaa cggtgctcaa
    10381 aggatgggat agccctaatt ccgcgtctcc ttgtgccacg ccgatacgcg cttcgccgaa
    10441 gtcgaatgcc agtgccgttc cttttggaat tttatgcatg gccggctcct gaaaacaggg
    10501 cgagcgggtc gatgccgagt ttggcgaatg cggcggcgta acggtgttcg taggggatgt
    10561 cgaacaggat gtgttcgtcg gcgggaacag tcagccacgc attgtcggca agttcgcgtt
    10621 cgagctgccc tttgctccag cttgaatagc ctatgctgat caaggctttg tcaaccgcac
    10681 cttcgcgtga aatattttca atcacgtctc gggaagaagt tagcgcgata ttgtctgaaa
    10741 cgccgatact gctttgccag ttgccgatcg gggtatgcac gacataaccg cgctcgacct
    10801 gcaccggacc gcccatcatc acgctgtcgt gctgcatccg catggggatg tttttgccgg
    10861 tggcggaaaa aatcatgtcc atcgtaatcg gagagggttt gttgatggcg atgccgagtg
    10921 cgccgtcttc atcgtgtttg cagatataga cgaccgattg tgaaaaaaac gcgtcttcca
    10981 tatcgggcat ggcgatgaga aagtggttgg agaggttcat atcagcgttt gtcgagattt
    11041 tctatcgggt tggaaatcat gcaggcgccg gcaagcaggt tgccggaagt atcggcttgc
    11101 accccatcgg tttgcggtat gccgccgccg aaacaggaaa ccgcgcttaa ggaatttttc
    11161 ggcgagcctt ggatgatttt atcgctgtac accaaatagg cgaaggtttt gcgtttgggg
    11221 tcgtaataac ggacaatctg ccggctcttg aacgcgaagc tcgcaccgtg tttgaaaact
    11281 tctttcggtt tgcgcacggc ggtttcgtca aaagaaatcg aagatgccgt ctgaacgcac
    11341 gaaaccgatg cgtcggacgc gtcctcttcc aaattgacca tttccttcaa gccgcctttt
    11401 tttgcatacg aaatataaca ggcaacccct tgaacgtcgg gatcgtcgaa tccttccact
    11461 tcgatacggt cgtttttgcc cagtatgttg aaaacggtac ttgcccgtcc gattttatcg
    11521 gtttcgccgc tgccgcaggc agtcagcagg acggcggcag acagcagtag cagtctgttc
    11581 atcaaaattt ccttatcggt tgaatccccg ttttccgtga aagcggcagg gcttgctttg
    11641 tttggagggg cgcacgttcc caaaatcggg caacatacgg tatgcggctt aatgtgctac
    11701 cctgtgtgtc ggaacacaat atccgttgcg gcattgaaaa tatgacgcac aatataaggc
    11761 tgtttgagcc gattgcaagt atccgcgcaa agaaggcgca agattgggca aacggcattt
    11821 tcagacggca taactgacag tataatccaa acgtccggct gctttcgtgc agccttcagt
    11881 attatccaac tttttccgaa ggtcgaacca tgcgctacaa accccttctg cttgccctga
    11941 tgctcgtttt ttccacgccc gccgttgccg cccacgacgc ggcacacaac cgttccgccg
    12001 aagtgaaaaa acagacgaag aacaaaaaag aacagcccga agcggcggaa ggcaaaaaag
    12061 aaaaaggcaa aaatggcgca gtgaaagata aaaaaacagg cggcaaagag gcggcaaaag
    12121 agggcaaaga gtccaaaaaa accgccaaaa accgcaaaga agcagagaag gaggcgacat
    12181 ccaggcagtc tgcgcgcaaa ggacgcgaag gggataagaa atcgaaggcg gaacacaaaa
    12241 aggcacatgg caagcccgtg tccggatcca aagaaaaaaa cgcaaaaaca cagcctgaaa
    12301 acaaacaagg caaaaaagag gcaaaaggac agggcaatcc gcgcaagggc ggcaaggcgg
    12361 aaaaagacac tgtttctgca aataaaaaag tccgttccga caagaacggc aaagcagtga
    12421 aacaggacaa aaaatacagg gaagagaaaa atgccaaaac cgattccgac gaattgaaag
    12481 ccgccgttgc cgctgccacc aatgatgtcg aaaacaaaaa agccctgctc aaacaaagcg
    12541 aaggaatgct gcttcatgtc agcaattccc tcaaacagct tcaggaagag cgtatccgcc
    12601 aagagcgtat ccgtcaggcg cgcggcaacc ttgcttccgt caaccgcaaa cagcgcgagg
    12661 cttgggacaa gttccaaaaa ctcaataccg agctgaaccg tttgaaaacg gaagtcgccg
    12721 ctacgaaagc gcagatttcc cgtttcgtat cggggaacta taaaaacagc cagccgaatg
    12781 cggttgccct gttcctgaaa aacgccgaac cgggtcagaa aaaccgcttt ttgcgttata
    12841 cgcgttatgt aaacgcctcc aatcgggaag ttgtcaagga tttggaaaaa cagcagaagg
    12901 ctttggcggt acaagagcag aaaatcaaca atgagcttgc ccgtttgaag aaaattcagg
    12961 caaacgtgca atctctgctg aaaaaacagg gtgtaaccga tgcggcggaa cagacggaaa
    13021 gccgcagaca gaatgccaaa atcgccaaag atgcccgaaa actgctggaa cagaaaggga
    13081 acgagcagca gctgaacaag ctcttgagca atttggagaa gaaaaaggcc gaacaccgca
    13141 ttcaggatgc ggaagcaaaa agaaaattgg ctgaagccag actggcggca gccgaaaaag
    13201 ccagaaaaga agcggcgcag cagaaggctg aagcacgacg tgcggaaatg tccaacctga
    13261 ccgccgaaga caggaacatc caagcgcctt cggttatggg tatcggcagt gccgacggtt
    13321 tcagccgcat gcaaggacgt ttgaaaaaac cggttgacgg tgtgccgacc ggacttttcg
    13381 ggcagaaccg gagcggcggc gatatttgga aaggcgtgtt ctattccact gcaccggcaa
    13441 cggttgaaag cattgcgccg ggaacggtaa gctatgcgga cgagttggac ggctacggca
    13501 aagtggtcgt ggtcgatcac ggcgagaact acatcagcat ctatgccggt ttgagcgaaa
    13561 tttccgtcgg caagggttat atggtcgcgg caggaagcaa aatcggctcg agcgggtcgc
    13621 tgccggacgg ggaagagggg ctttacctgc aaatacgtta tcaaggtcag gtattgaacc
    13681 cttcgagctg gatacgttga tcggattgcc gaaacggtag gctttatgca aatcgtgccg
    13741 atatggtgcg ctgttccgtg aagtttgccg tttgccgtac ctgtattttc cggtttttgg
    13801 aaatcaagag agaaagttaa agaagaatgt cgaaacctgt ttttaagaaa atcgcacttt
    13861 atactttggg tgcaatcagc ggcgtggccg tcagtctggc ggtgcagggt tttgccgccg
    13921 agaaggacag gcgggataac gaagtcctgc cggtgcaatc catccgcaca atggcggagg
    13981 tttacggtca aatcaaggca aactactatc aggacaaacc cgatgccgat ttgtttgaag
    14041 gtgcgatgaa gggtatggtg gccggtttgg atccgcattc cgaatatatg gataaaaaag
    14101 gttatgccga gataaaggag tccaccagcg gcgaatttgg cggcttgggg atggaaatcg
    14161 ggcaggaaga cggatttgtc aaagtggttt cgccgattga ggacacgcct gcggaacggg
    14221 cgggggtgaa aagcggcgat ttcattgtga aaatcgataa tgtttcgaca cgcggcatga
    14281 cggtcagcga agcggtgaag aaaatgcggg gcaagccggg tacgaagatt actttgacgc
    14341 tgtcgcgcaa aaatgccgac aagccgatag tcgtcaacct gacccgtgcc attattaaag
    14401 tgaaaagcgt ccgccatcac ctgatcgaac ccgattacgg ctatatccgc gtgtcgcagt
    14461 tccaagagcg gacggtcgaa agcgtcaata ccgccgcaaa agagctggta aaggaaaata
    14521 aaggaaaacc gctcaagggg ctggtgttgg atttgcgcga cgaccccggc gggcttttga
    14581 ctggcgcggt cggcgtgtcg gcggcatttc tgccgtctga agcagtcgtc gtcagcacca
    14641 agggacgcga cggcaaagac cgcatggtac tgaaagccat tcctgaagat tatgtgtacg
    14701 ggatgggcgg cgattcgttg gcgggcattc ctgccgagtt gaaaaccata ccgatgacgg
    14761 tattggtcaa ttccggttcg gcttccgcgt cggagattgt cgcaggtgca ttgcaggatc
    14821 ataaacgcgc ggtcatcgtc ggtacgcaga gcttcggcaa aggttcggtt cagactttga
    14881 ttcctttgtc caacggcagc gcggtcaagc tgacaacggc actgtattat acgccgaacg
    14941 accgttctat tcaggcgcag gggattgttc ccgatgtcga agtaaaagat aaggaacgca
    15001 tttttgaaag ccgcgaggcg gatttggtcg gacacatcgg caatcccttg ggcggcgagg
    15061 atgtgaacgg tgaaaccctt gccgtgccgc ttgaaaaaga tgcggataag cccgctgtaa
    15121 aagaaaaagg taaaaagaaa aaggacgagg atttgtcttc aaggcggatt cccaaccctg
    15181 ccaaagacga ccagttgcgg aaagctttgg atttagtcaa gtcgcccgag cagtggcaga
    15241 agtctttggg gctggcggcg aaaaagccgg tttcaaataa agataagaaa gataaaaaag
    15301 ataagaagta ggttttgacg atgccgtctg aaaggatttc agacggcata ttatgtttga
    15361 aaggacaggt tatgagcgat tttttaccgg aatttgaacc gtgcgg
//
LOCUS       DAWISC010000033        15306 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWISC010000033 DAWISC010000000
VERSION     DAWISC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.033"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..140)
                     /locus_tag="WIW87_000715"
     CDS             complement(<1..140)
                     /locus_tag="WIW87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ5173909.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WIW87_000716"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WIW87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ5173910.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ETVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WIW87_000717"
     CDS             1619..2668
                     /locus_tag="WIW87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5173911.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WIW87_000718"
     CDS             3356..4777
                     /locus_tag="WIW87_000718"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ5173912.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WIW87_000719"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WIW87_000719"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ5173913.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5626..5970
                     /locus_tag="WIW87_000720"
     CDS             5626..5970
                     /locus_tag="WIW87_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173914.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WIW87_000721"
     CDS             5981..6511
                     /locus_tag="WIW87_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5173915.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIW87_000722"
     CDS             complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIW87_000722"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ5173916.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRDVLDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADALADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            8030..8611
                     /locus_tag="WIW87_000723"
     CDS             8030..8611
                     /locus_tag="WIW87_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ5173917.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGSGEEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WIW87_000724"
     CDS             complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WIW87_000724"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ5173918.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9750..9827
                     /locus_tag="WIW87_000725"
     tRNA            9750..9827
                     /locus_tag="WIW87_000725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9785..9787,aa:Pro,seq:tgg)
     gene            9852..9928
                     /locus_tag="WIW87_000726"
     tRNA            9852..9928
                     /locus_tag="WIW87_000726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9886..9888,aa:Arg,seq:tct)
     gene            9956..10031
                     /locus_tag="WIW87_000727"
     tRNA            9956..10031
                     /locus_tag="WIW87_000727"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9989..9991,aa:His,seq:gtg)
     gene            10167..10673
                     /gene="rfaE2"
                     /locus_tag="WIW87_000728"
     CDS             10167..10673
                     /gene="rfaE2"
                     /locus_tag="WIW87_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEZ5173919.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10670..12448
                     /locus_tag="WIW87_000729"
     CDS             10670..12448
                     /locus_tag="WIW87_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ5173920.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIYGLLNMIAAEGREYEHI"
     gene            12460..13326
                     /locus_tag="WIW87_000730"
     CDS             12460..13326
                     /locus_tag="WIW87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ5173921.1"
                     /translation="MKWLFILLVAINIAVFGGTVGYKLTLKQAGRIPEAQNAANNLQV
                     QPVAPTMPVVRNIPASGPVVQAASESDTGALLKQGDILSEEQAEQLRLKKEAEQKKLK
                     EKKQREEKARREKLAAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHV
                     ASRSVEKRTAQAKPADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSR
                     ENAQALQNRLAGAGFGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKIT
                     SLYGKLNLKSCK"
     gene            complement(14092..14880)
                     /locus_tag="WIW87_000731"
     CDS             complement(14092..14880)
                     /locus_tag="WIW87_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ5173922.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15094..15288)
                     /gene="thiS"
                     /locus_tag="WIW87_000732"
     CDS             complement(15094..15288)
                     /gene="thiS"
                     /locus_tag="WIW87_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ5173923.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3667 a         3848 c         4249 g         3542 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcggacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggc agcgtctggg cagaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 acccttatcc gcgacctcga caccggcgaa accgttgccg acctcactta tcacggacag
      721 cgcgtctgcc tcgccaaagg cggcaaaggc ggcttgggca acatccactt caaatcgtcc
      781 gtcaaccgcg ccccgaaaca atccaccccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactta aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacttatcac gtaccggcct gctgctgcac gtcgtcgatt tggcgccctt cgacgaaacc
     1141 gtcaaccccg ccgaagaagc actcgccatc atcaacgaat tacgcaaata cgacgaagaa
     1201 ctctacggca aaccgcgctg gctggtactg aacaaactcg atatgcttga cgaagaagaa
     1261 gcccaaacgc gcactgccgc cttcctcgaa gccgtcggct gggactaccc caaacccgac
     1321 gaccgtttcc aatttgacat ggaaaccccg cgcctcttcc aaatcagtgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgtatc
     1441 gaagctgaaa aagcagaagc ggagaaagca gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacaggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggt aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatacctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgaa tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctaa cccgtcaaaa agaacccttt tcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtctgcg gtatgaccgt ttacgattac tgccatttgg gacacgcccg
     3481 cgtgatggtg gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcaaactga ctgcgcgttt catccaagcc atgcacgaag atgccgatgc
     3661 tttgggcgtg ttgcgtccgg atattgagcc gaaggcaacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaattg tcgggcaaat cgttggacga
     3841 cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gccgccaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gccgcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggccgt ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg atccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 tctctccaac gaagaaatcg aagacttgat cgcccggcgg aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttaaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatacat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcacgggat gcacggtcat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 gcaggttgag cgttatctcg ctttatttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 ggcgcgacat tgtcgtctgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caaccagaaa aattcaggtt tcctgcctga agagcgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacttg gtggagcaac cgagggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgctg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttctgaccat gcgccgctgg tcgtggagta tgactatgct gccgaataag
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc ggcatgggtc
     5701 gtcgctgcgt ttatcctgcc catgttggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg atatgggcgt tggtgtggct gctgatttgc
     5821 ctgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcaga cggcatttga acgcatacgc ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac gctttccaac gcgtccgctg tccctgccgt gccgcattgg ttgtacccga
     6361 acacggcggc gtgccgcacg gttgggcgca aaaacaggcg gatttgtgga acgcccgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggcatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcaatcggg ctgttttcat tgacgtgcag gcgcatcagg ttcggataaa
     6601 gtcgggctgc cgcataccgc cggaaatgga atcctttatt ttttacgtcg gcagtgccgg
     6661 cagggcgtta tccggcaggg ttgcaaggaa aatgccgtct gaaacaggct tcagacggca
     6721 tttccattca aacagggctc acaggctgcc caacacgata cgcaatacgc ggcgcagcgg
     6781 ttcggcagcg ccccacaaaa gttggtcgcc gacggtgaac gcgctgatgt attcgccgcc
     6841 catgcccagt ttgcggatgc gtccgacagg gacggacagc gtgccggtaa ctttggcagg
     6901 agtcagctcg tggatgctgg cttctttttc attggggatg actttcaccc agtcattcgc
     6961 gcctgccaaa atcgtttcga tttcggaaac aggcaggtct tttttcaact tcagagtgat
     7021 ggcttggctg tggcagcgca tcgcgccgac gcggacgcac aggccgtcaa tcacggttgg
     7081 attgtcgctg cggccgagga ttttgttggt ttccacgccg cctttccatt cttctttgga
     7141 ctggccgttg cccaaatcca cgtcaatcca cggaatcagg ctgccggcga gcggtacgcc
     7201 gaagttggct ttcggatagt cttcgctgcg caggaaatcg gatactttgc ggtcgatgtc
     7261 gagaatcgag ccggcaggat cggcaagcgc gtccgccact tgggcgtgaa ccgcgcccat
     7321 accgctgatg agttcgcgca tgtttttcgc gcccgcgccg gaagcggctt ggtaggtcat
     7381 gctggttgcc cattcgacca aatcgttttg gaacaggccg cccaaagcca tcagcatcag
     7441 ggaaacggtg cagttgccgc caatgtagtt tttcacgccg tttttgagac cgttgtcgag
     7501 gacatcgcgg ttgacagggt cgaggacgat aatcgcgtcg tctttcatgc gcagtgagga
     7561 cgccgcgtca atccagtagc cgttccagcc gctgtcgcgc agggcttgga agacggattt
     7621 ggtgtaatcg ccgccttggc aggtaacgat gatgtccatt ttggcgagtt cggcaacatt
     7681 gttggcatct aataatgttt tagccgcctg accgaaatca ggggctgcgc cgccgacgtt
     7741 ggaagtggta aagaaaaacg cttcaggaat gtgggcgaag tcgttttctt ctttcatacg
     7801 ctgcatcaaa accgaaccga ccataccgcg ccagccgacg aaacctactt tcattgtgtt
     7861 gctccttgag gaaaaatcgt taataaaatt atgtatggcg taaaggaagc gttttaacgc
     7921 cgtattggga aaatgtaaag ttctttatcg ggatttcttt aagtattggc gttttcggtg
     7981 tcgtggcggt agaatcgggg tcagttaaga acaattatca ggaacggata tggaagaaaa
     8041 aaacgactac accgacgcgg catccgacaa ccgcaacggg caggaaattg aagtcggaat
     8101 tgccggtgca ggcgaccgga tacttgccgc gctgctgaac caattgttta cctttttggt
     8161 tttgttggtg ccgtttgccg gactgattgc tttcgccatc aagaatgaag gcaggattgg
     8221 cagcggcgaa gaaatattcg ggctgctttt gggcatgact tccttttggg taggactggc
     8281 aggcattttg gcgtacaccg tcatccaaat ctactatatg agccgggacg ggcagtcatt
     8341 gggtaagaaa atcatgagaa tccgtgtgtt gaaaaccgac ggccgcaatc ccggttttgt
     8401 cggcacggtt ttggtacgcg aaatcgcatg gtcggttttg gttgccatta ttgccgccgt
     8461 tatcggtctt gcagtaggtg acaacggaga aaacgccatc aacctgctgg cattccttgc
     8521 caactttgtc ctgctcttta tggtcaaacg cgaccgccgc acgctttacg acatactggc
     8581 ggatacggtt gtcgtcaagc tgcccaaata aacggacacg gcaaaatgcc gtctgaaagc
     8641 ctttcagacg gcatttttat cttcagaacc gctcaagcat cgtgcagtga agccgcgtgc
     8701 agggtgtttt ccatcaatgt ggcaatcgtc atcggaccca cgccgccggg aacgggcgta
     8761 atcatcgccg cccgttcttt tgccgtttca aattccacgt cgccgcacag gctgccatcg
     8821 tccaaacggt tgatgcccac atcaataacg accgcgccag gtttgatcca ttcgcctttg
     8881 acaaagttcg gaatgcctac gccgaccacc aaaatatcgg ctccggcaac ctcgtctgtc
     8941 agattttcgg ttgcgctgtg gcagaccgtt accgttgcgc gcgccagcag cagttccaaa
     9001 gcctgcgggc ggccgacgat attcgacgcg ccgaccacga ccgctttttt ccccttcgga
     9061 tcaatgccgt aagcttccaa aagcgtcatc acgcccttgg gcgtacacgg gcgcatcagc
     9121 ggcattttga ccgccagcct gccgacattg taaggatgga agccgtccac gtccttatcc
     9181 ggcgaaatac gttccaaaac cgcctggctg tcgaggtgct tcggcagcgg tagctgaacc
     9241 agaataccgt ccacttcgga atcggcattc aggcggtcga ccagtgccag cagttcttcc
     9301 tgcgatgttg attcgggcag ctcgtaagac agtgatttga tgccgcattt ttggcaggca
     9361 gttttcttgt tgcggacata aaccgcgctg gcagggtcgc ctccgaccaa aaccacggcc
     9421 aggcaaggcg tgtgcagatt gttctgttgg cgttgcgcca ccgcttcggc aaccgcctgc
     9481 aggcgttttt gcgaaacttc tttaccattg atcagttgtg ccgacatatc cgtccttttg
     9541 ttaaacaggt agaaaatgct gaaaattatc gcattttccg tttgttttta cagcaggatg
     9601 ccgaaaataa atttgccctg aaataaaata cggctgtttg gacggattaa atattttttc
     9661 aaatcaaaaa actatatttt tcatcttggt gtcttgaaaa taaatgttga cgcatctttc
     9721 cctgagttgt atagttcgct tcttcaagtc ggggcgtagc gcagcccggt tagcgcatct
     9781 gctttgggag cagagggtcg tgagttcgaa tcccaccgcc ccgaccaatc aatcagcacg
     9841 aatcggcatg agcgcccgta gctcaaccgg atagagcacc gaccttctaa gtcgggggtt
     9901 acaggttcga ttcctgtcgg gcgtgccaag aatttgattg aaaaccgttt ttatggtggc
     9961 tgtagctcag ttggtagagc cccggattgt gattccggtt gtcgtgggtt cgagccccat
    10021 cagccacccc agataaaggc ccgtactttt cggtacgggt cttttcccgt ttgcgcgtac
    10081 tgtccgcgcc gccggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc
    10141 ttttatggtt tttgtcggag gctttgatgg ttgacgcttg gtctgtccct gattttgaat
    10201 cgaaaatctg tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg
    10261 tgtttaccaa cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg
    10321 cgcgttcgat gggggacgcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtc
    10381 tgggcaaggg cggtgaccgc ccggttaatc ctttggagaa ccgtgccgcc gttgccgccg
    10441 ctttggaaag tgtggatttg gtaacgtggt ttgacgggga tacgccggcg gcgttgattg
    10501 aggcggtcaa acctgagatt ttggtcaagg gcggcgattg ggctgcggat aagattgtcg
    10561 gtgcggcaga aacgttggcg cgcggcggtc aggtgttttc aattccgttt ctgcaccaga
    10621 cttcgacaac gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt
    10681 gaagctttcg cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtctc
    10741 gcaactggcg cgtatggcgg atatgaagcc gcagcagctc aacggttttt ggcagcagat
    10801 gccggcgcac atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc
    10861 attggcggtt ttcgatgccg aaggtttgcg cgagctgggg gaaaggtcgg gttttcagac
    10921 ggcattgaag cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc
    10981 gccggacaag gcgcacaaaa ccatatgcgt gacccacctg caaagtaagg gcagggggcg
    11041 gcaggggcgg aagtggtcgc accgtttggg cgagtgtctg atgttcagtt ttggctgggt
    11101 gtttgaccgg ccgcagtatg agttgggttc gctgtcgcct gttgcggcag tggcgtgtcg
    11161 gcgcgccttg tcgcgtttag gtttggatgt gcagattaag tggcccaatg atttggttgt
    11221 cggacgcgac aaattgggcg gcattctgat tgaaacggtc aggacgggcg gcaaaacggt
    11281 tgccgtggtc ggtatcggca tcaattttgt cctgcccaag gaagtagaaa atgccgcttc
    11341 cgtgcaatcg ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtgctgct
    11401 ggaaacgctg ttggtggaac tggacgcggt gttgttgcaa tatgcgcggg acggatttgc
    11461 gccttttgtg gcggaatatc aggctgccaa ccgcgaccac ggcaaggcgg tattgctgtt
    11521 gcgcgacggc gaaaccgtgt tcgaaggcac ggttaaaggc gtggacggac aaggcgtttt
    11581 gcacttggaa acggcagagg gcaaacagac ggtcgtcagc ggcgaaatca gcctgcggtc
    11641 cgacgacagg ccggtttccg tgccgaagcg gcgggattcg gaacgttttc tgctgttgga
    11701 cggcggcaac agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg
    11761 tagcgcgccg taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg
    11821 aaatgtccgc atcgtcggtt gcgctgtgtg cggagaattc aaaaaggcac aagtgcagga
    11881 acagctcgcc cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa
    11941 ccactaccgc caccccgaag aacacggttc cgaccgctgg ttcaacgcct tgggcagccg
    12001 ccgcttcagc cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc
    12061 gctcaccgat gacggacatt atctcggggg aaccatcatg cccggtttcc acctgatgaa
    12121 agaatcgctc gccgtccgaa ccgccaacct caaccggcac gccggtaagc gttatccttt
    12181 cccgaccaca acgggcaatg ccgtcgccag cggcatgatg gatgcggttt gcggctcggt
    12241 tatgatgatg cacgggcgtt tgaaagaaaa aaccggggcg ggcaagcctg tcgatgtcat
    12301 cattaccggc ggcggcgcgg caaaagttgc cgaagccctg ccgcctgcat ttttggcgga
    12361 aaataccgtg cgcgtggcgg acaacctcgt catttacggg ttgttgaaca tgattgccgc
    12421 cgaaggcagg gaatatgaac atatttaagg aatacagaga tgaaatggct atttatcctt
    12481 ttggttgcga ttaatattgc cgtattcggc ggtacggtag gttacaaact gacactgaaa
    12541 caggccggca gaataccgga ggcacagaat gccgcaaaca atttgcaggt tcaaccagtt
    12601 gccccaacta tgccggttgt tcggaatatt ccagcatccg gtcctgtcgt tcaggcggca
    12661 tctgaatcgg atacaggcgc actgctcaaa cagggcgaca ttctgagcga agaacaggcg
    12721 gagcagttgc gcttgaaaaa agaagcggaa cagaaaaaac tgaaagagaa aaaacagcgt
    12781 gaagaaaaag cccgccgcga aaaactcgcc gccgaaaagg cgcaggcgga acgcgaaaac
    12841 ggcgcggcgg atgccttatg cgccgcgcag gcaagcctca cgatggacga agatgactac
    12901 caccgcatca aaggactttt gggcaaatgg tcgcacgttg ccagcaggag cgtcgaaaaa
    12961 cgcaccgccc aagccaaacc tgccgacaaa acctaccgcg tcgtcctgcc cgtttccgcc
    13021 gatgccgaaa atcaggcggc ggagctgtct gccaaaggtt tcaaccccat accgtttgac
    13081 ggcgcattga gtttgggtgt cggcaacagc cgggaaaacg cccaagccct gcaaaaccgg
    13141 cttgccggcg ccggattcgg cggggcgcat attgtcgaac actttgccga agccgacagg
    13201 caggacgatt ctttgagcgt gtcgcgtatg acggttttgt ttaccggcgt gaatgccgcc
    13261 gatgcggacg aaattcgtaa aatcacgtcc ctatacggca aactgaacct caagtcttgc
    13321 aaataggcgc gaaagccgga cgataaacgc caaacccacc gcgaaggtgg gtttggcgtt
    13381 tcggttttga gtatctgata tataaaaagg gaatgtatgt ctcgtatatg ccgactccaa
    13441 gtgtgaaagt gatgatgggg aaatatggtg tttaatggtt cggaacggac ggcttccgag
    13501 tggttttgat tatacgctaa taataataat aattatcaat aaaaatttaa aaataaatgc
    13561 agattttttg taatacgatg aaaaataaac aaattaacct gtccgccgat gtttgtgatt
    13621 cgggtcggca aactttcatt gtgtcggaca aatcggggtc ggcaggcata gctgagggct
    13681 tcgtgcctga cggaaataag gttgcccgtg tttgaacatc cgtccgattt ggcgcggaca
    13741 gggttgtcat tcgggggtgt ttgagcgtgg attcggggat gcgaaagcgg taaaaccgtt
    13801 gcggatgaag gcttacggac ggctttaggt atttttaagt gtattcaaac aacaacaaaa
    13861 tgcttcttct attgccgtat ttagggcttg cgcggcagcc tgcacaccgt ggaggccgag
    13921 tgtttcgttg gttgcggcat cgggacgggc ggcaaggttt tccggccggt ttttccattt
    13981 tttccgacaa agccgtgatg ccggggaatg gcggtttgtc cgtttcaggc cgccgccgtt
    14041 ttggaccgcg atgccgtctg aagccttcag acggcatttt tgctgccttt ttcaatattc
    14101 cgccgaatgc caaaacggtt gtccgactgt cggcgtgctg gcttgcgctt tgtcgcgtgc
    14161 ttcgaccggt ccggcttcaa atgccagccg tccggattcg acggcgagtg cgaaggcgcg
    14221 tgccatattg accggatcgc cgctgcggga aacggcagta ttcaaaagca cgccgtcaaa
    14281 gccccattcc atcacttgtg ccgcctgtga gggcaaaccc aagcccgcgt cgataatcag
    14341 cggcgtgtcg ggcaggcgtt cgcgcaggac gttcaacgcg taggcgtgaa ccgcgcccaa
    14401 acccgtgccg atcggggccg cccacggcat caacgcctga cagcccgcgt cgagcaggcg
    14461 gcggcaggca atcaggtctt cggtgcaata aggcagcact ttgaagccgt ctttaatcag
    14521 gatttccgcc gcttcgacaa gctggaacac atccggctgc aaggtgtcgt catctccgat
    14581 gagttccaat tttatccaat cggtttcaaa cacttcgcgc gccatttgcg ccgtcgttac
    14641 cgcttcctgc acgctttggc agcctgccgt gttcggcagg acgggaacgc cggtttcttg
    14701 aagcagcgac caaaacccct gaccgtgcgc ctcgccgccg cttcccgcgc ggcgcagcga
    14761 gacggtaatc atcgcaggct gggcggtttg gatggattgt ttgaggattt cgggggtcgg
    14821 gtaggcagcc gtgccgagca gcagccgcga ggggaaagtt tcgccgtata gggtgagcat
    14881 aatgggttcc tttgtatagt ggattaacaa aaaccagtac ggcgttgcct cgccttagct
    14941 caaagaaaac gattctctaa ggtgctgaag cactaagtga atcggttccg tactatccgt
    15001 actgtctgcg gcttcgtcgc cttgtcctga tttaaagtta atccaccata acgttgtttt
    15061 ggggacaggg gtcgtctgaa aaggcaaaac cgcctagccg ccgaccaccg gccgcacgat
    15121 atcgattttg tcgttttcgt ttaaaaccgt ttccgcatac gcgcctttgg ggacgaaaac
    15181 ggtgttgacc gccacggcaa agggcttttg cggcgcggtt tgggcgatga ggtcggcaac
    15241 ggtcgtgccg tgaagttcgg cgggtccgcc gtttaagatg atgttcatgg tttctcgatg
    15301 tttctg
//
LOCUS       DAWISC010000034        14611 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWISC010000034 DAWISC010000000
VERSION     DAWISC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.034"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WIW87_000733"
     CDS             complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WIW87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ5173924.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(319..1374)
                     /locus_tag="WIW87_000734"
     CDS             complement(319..1374)
                     /locus_tag="WIW87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ5173925.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WIW87_000735"
     CDS             complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WIW87_000735"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ5173926.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WIW87_000736"
     CDS             complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WIW87_000736"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ5173927.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIAEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3355..3981)
                     /locus_tag="WIW87_000737"
     CDS             complement(3355..3981)
                     /locus_tag="WIW87_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ5173928.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3981..4757)
                     /locus_tag="WIW87_000738"
     CDS             complement(3981..4757)
                     /locus_tag="WIW87_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ5173929.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKTEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4750..5982)
                     /locus_tag="WIW87_000739"
     CDS             complement(4750..5982)
                     /locus_tag="WIW87_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ5173930.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQNIANDWHYAFAN
                     ALGINMSSEAGVSDKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5985..7328)
                     /locus_tag="WIW87_000740"
     CDS             complement(5985..7328)
                     /locus_tag="WIW87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ5173931.1"
                     /translation="MIKIKKGLNLPIAGRPEQAVYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVITIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDTDNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7678..9099)
                     /locus_tag="WIW87_000741"
     CDS             complement(7678..9099)
                     /locus_tag="WIW87_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ5173932.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9460..9819)
                     /locus_tag="WIW87_000742"
     CDS             complement(9460..9819)
                     /locus_tag="WIW87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ5173933.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9816..10421)
                     /locus_tag="WIW87_000743"
     CDS             complement(9816..10421)
                     /locus_tag="WIW87_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ5173934.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10468..10950)
                     /locus_tag="WIW87_000744"
     CDS             complement(10468..10950)
                     /locus_tag="WIW87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ5173935.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11153..11250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11461..12561
                     /gene="gcvT"
                     /locus_tag="WIW87_000745"
     CDS             11461..12561
                     /gene="gcvT"
                     /locus_tag="WIW87_000745"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ5173936.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12723..13109
                     /gene="gcvH"
                     /locus_tag="WIW87_000746"
     CDS             12723..13109
                     /gene="gcvH"
                     /locus_tag="WIW87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ5173937.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            13261..14508
                     /locus_tag="WIW87_000747"
     CDS             13261..14508
                     /locus_tag="WIW87_000747"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ5173938.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         3541 a         3624 c         4023 g         3423 t
ORIGIN      
        1 accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg tttttgttaa
       61 tccactatat ttcggcgtta acggtcaacc cgtatgccgc ctgcctgttt ttcttcatcc
      121 agtttctttt gcagggtgtc gcaaggtgtg tcgcagtcgc acattttttt catacccaag
      181 gcagtaatgc cgccgcaact gcctttgatg ctgcgtttgg agaaaatata gccgaccgcc
      241 ataccgatga tgacggtcag gaagatgccg aaggtaagga gcagggtttt catggtgttt
      301 cctaatcggt ttgtatgttt agcggagcag tttttcaaat tcggaagaca tggcggtgcg
      361 gtagccgcct ttatccctga caatcaggaa aacagcgagt ttttcgcgct ctgccagctt
      421 taaggcttcg gtttcgccca atacgaataa tcctgtggac aagccgtccg ccgtcatcgc
      481 actgtctgcg accacgctga tggaggcgag gttgtggctg atgggtcgtt tgttgttcgg
      541 gttgatgata tgggagaggc gtttgccgtt tttatcgacg tggaaaatac ggtaatcgcc
      601 ggaagtggca agcgaacggt tgttcagcgg gacgataatc tgcgtattgc cgccttggac
      661 gatattgggc tgctcgatac cgatgcgcca cggttcgccg cgcgcgtttt tgcctttgcc
      721 gtgcaactcg ccgccgattt cgaccagata attttgaatg ccgtattttt ccagttcgcc
      781 cgcaacttta tcaacgccga agcctttggc aatcgaagat aaatccaaat aggccttggg
      841 gtgggttttg ctcaaggaag cgtaatcttt gccttgtttc aaaatgattt tgtctatgcc
      901 cgtataagat gccgcctgtt tgatttgttc cggcgacggt tcacgggtaa cggatttgtc
      961 ggggccgaat ccccaaaggt tgaccaaggg gccgacggtt acgtccagcg cgccgtgtgt
     1021 caggcggttc aggcggacgg cttcggcagt aacgtgtgcg aagtcgcttg aaatgcggag
     1081 gggcttgccg gctgtgtgtt ggttgaaccg gctgatttcg gagtcgggct gataggtgga
     1141 catctgccgg ttgacttctt taagcgcgtc atcgatgcgt ttttgtattt cggcaggtga
     1201 ggggagtttg tcccgattat ttgaaaggta tttgacggta taggtcgtgc ccatcgtttc
     1261 gccttgcagg gtaacggttt gcgcggtttg ttccgaacag gcgttcagga agatgaaacc
     1321 cagggcaaat atcaagacgc ggataaagtt cggcaggcgt gtttcagacg gcatagtgtt
     1381 tgacggtttt ggcaaatggt ttgaattata tcgcaaaacg gccggtatgt ttctatgccg
     1441 atgccgtctg aagggtgttc ggatggcatc ggcatagaaa aaggaagaaa ccgaggtttc
     1501 ttccttttgt atttgaagcc gaatatttaa ccgccgaaat cgtccaagag gatgttttcg
     1561 tcttccacac ccaagtcttt gagcattttg atgacggact ggttcataat cggagggccg
     1621 cacatataaa attcgcagtc ttccggtgct tcgtggtttt tcaggtggtt ttcgtaaacc
     1681 acgttgtgaa tgaagcccgt gtagccgtcc cagttgtctt ccggcagcgg gtcggacagg
     1741 gcgacgtgcc acgtgaagtt cgggaactct gccgcgagtt ggtcaaagtc ttcgacatag
     1801 aacatctcgc gtttggaacg tgcgccgtac cagaaggtaa tcttacgttt ggagttcaaa
     1861 cgtttcaact ggtcgaaaat gtgggaacgc atcggagcca tacccgcacc gccgccgata
     1921 aataccattt cggcatcggt gtctttggcg aaaaattcgc cgaacgggcc ggaaatcgta
     1981 actttgtcgc cgggtttgag cgaccagatg taggacgaca tttgtcccgg aggcgcatca
     2041 ggtacgcgcg ggggcggcgt ggcgatacgc acgttcagca taatgatgcc tttttcttca
     2101 ggatacgaag ccatagagta ggcacgcaaa atcggctcgt ccactttgga aacgtattgc
     2161 cacaaattgt atttgtccca gtcttcgtga tattccttag gaatgtcgaa gtctttgtag
     2221 gcaacagtgt gaggaggagc ttcaatttga atgtagccgc cggcgcggaa ggggacttct
     2281 tcgccttcgg gaatggcaag cttgagttct ttaatgaacg tggctttgtt atcgttggag
     2341 atgacggtgc attcccattt tttcacgccg aacacttctt cggggacttc gatgtccatg
     2401 tcggttttga cgttgacttg gcacgacaga cggcagcctt cgcgtgcttc gcgtttgctg
     2461 atgtgggaca gctcggtcgg caggatgtcg ccgccgccgc tttttacgac gacgcggcat
     2521 tgtccgcacg aaccgccccc gccgcaggcg gaggggataa agatgccttc gttggcaagc
     2581 gcgcccaaga gtttgccgcc ggcgggcatc gtcagctctt tttcgccgtt gactttgatg
     2641 gtgatgtcgc cttcgctgac cagtttggat ttggcaaaca gaatcatcag tgccaaaacc
     2701 aaaacgatga cggtaaacat cacgatacct aaaataatct ccataccgat ccctttctta
     2761 taactggatg ccagagaacg acataaacgc catcgccatc aggccggcgg cgataaaggt
     2821 aatgcccaag cctttgaggc ctttgggagc gtccgaatat ttcatttttt cggtaatgcc
     2881 cgccaaagcg acaatcgcca acatccagcc caagcccgcg ccgaagccgt atacaacgga
     2941 ctcgccgaag ttgtattcgc gttgcgccat aaacgatacg gcgccgaaaa tcgcgcagtt
     3001 cacggtaatc agcggcaggt agatgcccaa tgcgttatag agggcgggga cgaatttatc
     3061 caagaacatt tccaaaatct gcaccaaagc ggcaatcacg ccgatgaagg tgatgaattt
     3121 caaaaaggtc aaatccacgc cttcggcaat cgcgccgtct ttgagcagcg agtaaacgag
     3181 ttggttgaca gggacggaca gcccgagtac gaaaattacc gccacaccca aaccgaatgc
     3241 ggtggatact tttttggata ccgccaaaaa cgtgcacata cccaaaaaga aggatagtgc
     3301 catattttca atgaagacgg atttgatgaa gaggctcaaa tagtgttcca tagcttattc
     3361 ctccgcctgt tcgggtttcc aggtacgcag tccccaaatc aaaaagccga tgatgaagaa
     3421 cgcgctgggg gcgagcagga acaagccgtt ggtctgatac cagccgccgt cctgcacggt
     3481 ttggaaaacg gtgtagccca agagtttgcc cgagccaatc agttcgcgga cggtggcgac
     3541 gacaagcagc attatcccgt agcccgcgcc gttgccgatg ccgtcgatca ggctttccag
     3601 cggcggctct ttcatcgcaa atgcttcggc gcggcccatc acgatacagt tggtaataat
     3661 cagaccgacg aatacggaaa gctgtttgga caattcgtag gcaaatgcct gcaagagttg
     3721 gtcgaccagc gtaaccagcg acgcgataat cgccatttgc acgataatac ggatgctgtt
     3781 ggggatgtag ttgcgtacca gcgaaatgaa gaagctggaa aaaccggtta ccaaagctac
     3841 ggaaataccc atcacgatgg ccgtctgaag tttggtggta accgccaaag ccgaacaaat
     3901 acccaaaacc tgcaaggcaa tcgggttgtt gtcgataaag ggtgaaaaca tcaaatgttt
     3961 caagcgtttc atatcagcca ttattgcgct cctgctgatt tcaatttgtt caggtagggg
     4021 atatagccgt tttcgccgaa ccagtaggcg aacgaacctt gcacgccttt ggatgtcagc
     4081 gatgcgccgg agagggcatc tacgccgtgt tctttgtccg aacccgcgcc tttgccgacg
     4141 tgcagggcga gtttgccttg tccgtcaaac agttttttgc cgacgaattt ttgctgccac
     4201 aacggattgc cgatttcgcc gcccaagccc ggggtttcgc cttgttcgta gtaggtaatg
     4261 ccgttgatgg tgttgccgtc gggctggatg gcgacaaagc cgtacatgac cgaccacaaa
     4321 ccgttaccgt gcataggcag gatgatttgc ccgattttgc cgtcttcgcc ttttaccaaa
     4381 taaacctcgg tgtatttggc acggcttttg atgcctgcca aatcgtcttc ggttttgatg
     4441 cggatgcttt gggcagggtc tttgcctgcg atgcgcgcgc tgaagtcttt aggcgcatcg
     4501 gcgacgtatt cgccggtcgc caaatcgaca acacgttgct cgatacgctc ggcaaaggtt
     4561 ttaccgatgt cggtgtcctt atccatcaaa ccggctacgc tcaagatata gccttgtttg
     4621 tcttggagtt tttgtttctc ttggatgggt ttcaagccga cgaccgcacc ggcaacgatg
     4681 accgagcaaa tcaggctgac cgccaacacg acaatcagcg tgccgctgaa gctgtcttta
     4741 tcgaatttct tagccattgc tgcgcgcctt tctgcgtttg atgttcgctt gtgcgacgaa
     4801 atagtcgaaa atcggggcaa acaggttggc aaacagaatc gccaacatca tgccttcggg
     4861 gtaagccgga ttgaccacgc ggattaatac gcacatcaca ccgatcagtg cgccgtacca
     4921 ccatttgccg acattggtaa aggaagcgga aacagggtcg gtcgccataa acagcatacc
     4981 gatggcgaag ccgccgacca ccaagtgcca gtaccaaggc atagcaaaca tagcgttggt
     5041 gtccgaaccg atgaagttga acagcgaaga catcgcaatc ataccgatca tcacgccggc
     5101 aataatgcgc caagaagcga tgcgggcaaa cacgataaac gcgccgccga ttaagagtgc
     5161 caaagtggag acttcgccaa tggagccggg cagtttgccg ataaacgcgt ccatccaagt
     5221 gatggtttga ccggttacgg cgtttttcag gccgtctgca ccgtgtgccg cccattgcgc
     5281 cagtgcggtt gcgccggaat agccgtcaac cgccgtccaa accgcatcgc cgctcaagtt
     5341 ggcagggtag gcgaagaaca ggaaagcacg gcctgccagc gcagggttca tgaagttttt
     5401 acctgtaccg ccgaatactt ctttcgcaac cacaacgccg aaagaaatac ccaaagccgc
     5461 ctgccacagc ggcagcgtgg gcggaacgat taaggcaaac agaatcgaag taacgaagaa
     5521 accttcgttg atttcgtgtt tgcgcacggt ggcgaacaaa acttcccaga aaccgcccac
     5581 aacaaataca gtcgcgtaaa tcggcaggaa gtaaatcgcg ccaaacagca ttttgtccga
     5641 cacgcccgct tcagacgaca tattgatgcc caaagcgttg gcaaaggcgt aatgccagtc
     5701 gttggcgatg ttttgttgca gcaaatcagg cgttaacgca ccgaatgcct gcgcgccgac
     5761 gttgtacata ccgtagaaca tggcagggaa caaagccagc cacaccaaaa tcatcatgcg
     5821 cttggagtcg agcgcgtcgc ggacgtgcgc cgctttgcgc gttaccgcgc cggatgtgta
     5881 gaaaattgtc gccgcagctt cgtagagggc ataccatttt tcatgtttgc cgcccggcag
     5941 gaagtgcggt tcgatttttt ccagaaaatg tttcaagccc ataatcagcc ttccttctca
     6001 atggtttcca gcactttgcg caacagcggg ccgtattcgt atttgcccgg gcagacgaag
     6061 ctgcacaaag cgaggtcttc ttcgtccaat tccaagcaac ccaatgcctg cgcgctgtcg
     6121 gtatcgccga cgattaaatc gcgcaaaagc agggtgggca ggatatccaa gggcatcacg
     6181 cgctcgtaag taccaatcgg caccatggcg cggtcgccgc cgttgacggc tgtgttgaac
     6241 ttgaagagtt tgtttttcag gaaatggccg agggttgtac gcgtgatgga gtatttgtcc
     6301 ggctgcggcg caacccagcc gaacagctct ttgctgcggc cttcttcgat aacggaaatc
     6361 tgattgtggt agcgtcccaa ataatcgtgc gcgccttgtg taatcgcgcc gttcaatacc
     6421 gaaccggaaa tcacgcggtt gtctgtgtca accaattcgc ccgcagtaat ttgcgatact
     6481 ttcgcaccca aaacggtacg caagaggcgc ggtttgttga cttgagaacc acctagggca
     6541 atcacgcgct cggtgttcag acggcctgtt gcaaacaaac ggccaatggt aattacatct
     6601 tgataattga tggtccacac ggttttattc gcgccgaccg gctcgatgaa atgaatgtgc
     6661 gtgccactca aaccggcagg atgcgggccg ccgaattcat gtgtttcgat gttggcagca
     6721 ttttcagacg gcacgtctgc gccagctgcc ttacaaacat ggattttgcg ttcggtcaaa
     6781 cggctcaata ccaacaggcc gcgtttgaaa tcctcggcgg cttctttgat aatgaccgta
     6841 gggtcggcag ccagcggatt ggtgtccatc gcattgacga agatggcgaa cggctcggca
     6901 tcgacggcag gaattttgct gaacggacgg gtgcgcagcg cagtccacaa accggattgg
     6961 atcaggttgc ggcgcacttc ttcgccgctt aagtttgcca gcgcttcagg tgcgtagcgt
     7021 tcaaactcga tttcgtcgtt gccttcaacg gcaatcacga ctgactgaag tacgcgcttt
     7081 tcgccacggt gaatcgcggc gattttgcct gaagccggcg cagtaaacac cacgcccgga
     7141 ttctttttgt cttcaaacag cacttggcct tttttgacgg catcgccttc cttgactttc
     7201 atcgaggggc gcataccggc atattcttcg ccaagcaacg cgacttcggt aatggccggg
     7261 ccgtcgtaaa cggcttgctc cggtctgccc gcgatgggca ggtttagacc ttttttgatt
     7321 ttaatcatat atttgcatta cttgtgatgg ttaaggtaaa aacggcgtgt tttgataccg
     7381 tgtcgcgtgg catcaaaagc attgaataaa ttaatgtagc aaagtgttag attctatcag
     7441 gaattgtacc tgtttgtcag atttgctgct tttttccttg cggaagccgt ttttatagtg
     7501 gattaaattt aaaccagtac ggcgttgcct cgcccttgcc gtactatttg tactgtctgc
     7561 ggcttcgtcg ccttgtcctg atttttgtta atccactata aattgtcgga aggggggata
     7621 ttgatttgat tatgccggaa tttaaaatgc cgtctgaatg ttcagacggc atagcgttta
     7681 cagcagtttg aaaacgaaaa agataagggt atgtacgatg aagacgggtg tcaggaaggc
     7741 gaccgaccac atcatatagc cgaagaaagt cggcatcggt acgccgcgct gttcggcaat
     7801 ggccttgacc atgaagttcg gtgcgttgcc gatgtaggtc agtgcgccca tgaataccga
     7861 acccatagaa accgccagca gcgaatgaaa cagggtaccc gtcatcaagg cttgggcatc
     7921 gccgcccgcc atattgaaaa aaacgagata agtgggcgcg ttatccaaga atgccgacaa
     7981 tatgccgctc atccaaaaat acatcacatt aatcggatga cctgccgtat cgtgaaccag
     8041 cgataccacc ccgcccagcg cgcctgcctc gcctgctttc agaatgctca ggacgggaaa
     8101 gatggtgatg aagatgccga ggaagagttt gcccacttcg gcgatgggtt caaagttgaa
     8161 ttcgttgcct gcgcggactt gtttgggcgt gattgccata gatacggcgg tcaatgcaat
     8221 caggatgaca tcgcggacga ggttttgcag ggcgtaacgg ctgccgagga tttcaaatcc
     8281 cgggtgttcg ggtttccaaa ggccggacat tagaaccgcg ccgaccacgc ccgaaagcag
     8341 gaggaagttc catttgccga agatggcgat tttttcgggt ttttcctgtt gtgccggcgt
     8401 atcttgtgca atgctttcct gtttgaagaa acggttgtcg atgaaataga aggcggtcaa
     8461 caggacagcg gtgctgatca ggacgggggc gaacatatgt ttgaccgtcc acatgaaatc
     8521 tacgcctttg aggaagccga ggaagagtgg ggggtcgccc aaaggggtca gaccgccgcc
     8581 gatgtttgca accaggaaaa tgaagaagat gacgatgtgc acgcggcggg tacggttttg
     8641 gttggctttc agcagcggac gaatcatcag cattgctgcg ccggtcgttc ccatgataga
     8701 ggcaagtgcc gtaccgacgg caagcagggc ggtgttgagc ttgggtgtgc cgttcaagtc
     8761 gccccaaacc aaaatgccgc ctgaaatggt gtacagggca agcagcagca ggatgaaagg
     8821 gatgtattct tcaacgagtg cgtgtgcgac ggtatggata ccggcggacg cgccaaaaac
     8881 caaactgaac gggatgagga agagcaatgt ccaaaaggcg gtaattttgc cgtaatggtg
     8941 atgccaggta tgcgaaaaaa acaagggacc caatgcgata gacagcaaaa tcagggcaaa
     9001 gggcaggccc cacagcaggt ttaggtttgc gccgtccaaa tctgcggcgt aaaccgatgc
     9061 tgggaaaagc attagtgaaa acaggggtag gtggcgcatc gtgtttcctc gattcaagca
     9121 ctgccttgcg cggcgcgtgg gagtgataca ggcaccgtgc cgcccggaca taggcggact
     9181 gtgctgcctg tttgtttttg aaaaaagttt tccgattgat tgtaaagtaa ataatcagga
     9241 taaaccagtt tcccaaaccg gaaggcggcg ggaaggcgga ttgctgtgct tgggaatata
     9301 tgttcttttt gataaataat tttatttaaa acaaataaat atatgtcttt aataaataat
     9361 ctatcgaaaa cgaaaaatga atttatttta acatatattt gcaatgaaac aggtttgccc
     9421 ccccccgttt gtttgccctt atccctttca gtacggcatt caagattcgg gcctgcgcca
     9481 catccatatg gcgacaaggg aacaaaaaac cgatgaaacc gccccgaccc accagcgttg
     9541 gggaaactgc caaaacatta tcaggcagga tgcggtcatc atactgatgg cgaatatttt
     9601 ggctttgcgc ggcactgcgc cgttttgttc ccagttatga accatcgggc cgaaatagcg
     9661 gtgccggtgc agccagcggt aaaagcgcgg ggatgccttt gcccagcagg cggcggagag
     9721 cagtacgaac ggcgtggtcg gcaacagcgg caaaaaaatg ccgatgatac ccaacagtag
     9781 ggaaatgcag ccgcaggcaa ttaaaagata acgtatcatt ttgaaatatt tttcttattg
     9841 tgcggataag ggcaggatgt gataccgagt tttgcccagc cttcatgtcc cattttttcc
     9901 agcagggcga tattgcgttc gaatattgcc gaagcgtcgg gaaaggcttg tgcggctttg
     9961 gcaatgctgt cttcgcggat gaggtgcagc gtcggatagg gagaacggtt ggtgtagttg
    10021 ccaatgtcgt ctgaatccgt gccttcaaat tggaaatcgg gatgaaacgg ggcgatttgg
    10081 acgatgcctt ctaagccgtt ttcgacaacg gcggcatcgg caatgtcgag catatcgttg
    10141 aatacgtcga aatcggggaa tagggtcggg tgaaccagca gggtggtttc cagttcggtg
    10201 gcgggtgtat tgcccagtcg ctgcagttct tcgtccaagt cttccaaaaa accgtcaagg
    10261 tgtttggctt cgctgatcgc gatgcggaca aggtttttaa cgtggggggc tttggcaaag
    10321 ggacacaggt tcagaccgat gacggctttt tccaaccatt gtccggtgtg ttcggcaaca
    10381 gcatctttat tttcggaagt attgatattc attattgtca tgtaaatgtg tttgcagatt
    10441 gcacgtgcgg gaaaatcggg aagggcacta ttccttcagc aggtggttga gcggcaggga
    10501 ggtggtgtgt ttgatttctt ttaaaacaaa gctcgattgc gcatcttgta cgccgtggtg
    10561 ggacaggagc gtatccaaaa caaaatggga aaacgcgttc atatcggtaa aaaacgcctg
    10621 aagcaggtag tcggtttccc ctgtcagggc gaagcagctc aagacttcag gccattttcg
    10681 aaccgatgcg gcaaagtctt cccgcgcgtc ttttgctttg cggatggaaa cgcggataaa
    10741 tgcctgaagt cccaagttga cagattccgg agacagcagc gcggcatatt ggcggacgat
    10801 accggcatct tccaactgct tcagacggcg caggcacgga gaaggcgaaa gtgcgacacg
    10861 ttcggacagt tcgacattgg tcagcctgcc gttttcctgg agaacctgta agattttaat
    10921 atcggttttg tctaaagtga gttggggcat atttgcgttc cgttttaagg aattcggatt
    10981 gtctgtccgt atgtttgcgg caatccgcac agatggagac catattaaca tataaaaagt
    11041 tataccgtca tccgggacaa attttgtttt cggaaaatca tgtgaaaaca gaggcggtcg
    11101 gtttgcatct ctttaagacg gcttgcccaa accgccgatt caagacataa tcgggaaatg
    11161 tgcaggagag tgttacaccc aactacaatg taaccaccga aggcgcagac acccttaaat
    11221 cgctcaggta tcagggactg cacattgaaa caaacaatct ggagagcggc gttggaataa
    11281 cgtccaccga aggggagaag gccgtctgaa ccaccattca gacaaccgcg caaagcagtg
    11341 agcagactgg tttgccatca tgcggataca gccgaaaatc tcaggttcaa ggacagatag
    11401 ggtcatccgc gcacaggtgc gcgggcggca tctgaacaaa aaatccggag aaacttgaga
    11461 atgactgctc tgaaaaccac cccatttcat caagcccatc aagatgcagg cgcgaagctg
    11521 gtcgattttg ccggctggga gctgcccatc cattatggtt cacaaatcgc cgaacacgaa
    11581 gccgtgcgca ccgacgccgg tatgtttgac gtatcccata tgctcgttac cgacgtagca
    11641 ggcgcaaatg ccaaagcctt tttccgcaaa ttgattgcca acgatgtcgc caagctcgct
    11701 tttgtcggca aagcccttta ttccgctttg ctcaacgaca acggcggtgt gattgacgac
    11761 ttaatcgttt accgcaccaa tgaagccgaa acccaatacc gcatcgtgtc caacggcgcg
    11821 acccgcgaaa aagacacggc gcaattccac aaagtcggac aagagttcgg cgtcgccttc
    11881 aatccgcgct acgacctcgg catgctcgcc gtacaaggcc ctaaagccat tgaaaaactc
    11941 ctgaccgtca aacccgaatg ggcagatgtc gtccataacc tcaaaccgtt ccaaggcgcg
    12001 gatttgggca acgactggtt tgtcgcccgc accggctaca ccggcgaaga cggcgtcgaa
    12061 gtcatcctgc ccggcaccga agccgtcgca ttcttcaaag ccctgcaaca agccggcgta
    12121 cagccctgcg gcctcggcgc gcgcgacacc ctgcgcatgg aagccggcat gaacctctac
    12181 ggcaacgata tggacgacga caccagcccg ctcgaagcag gtatgggttg gaccgttgac
    12241 ttgaaagacg aaagccgcga cttcgtcggc aaagccgcct tgctggcatt gaaagaaaaa
    12301 ggcgttgccg tcaaacaggt cggcctgttg ctcgaaaaag gcggcatcct gcgcgcgcat
    12361 atggaagtgt tgaccgacaa aggccaaggc gaaaccacca gcggcgtatt ctccccaagc
    12421 ctgaaacaat ccatcgccat cgcgcgcgta ccgaaagatt ttgacggcga taccgccaaa
    12481 gtgctgatgc gtggcaaaga agtggacgtg cgtgtactga agctgccgtt tgtccgcaac
    12541 ggacagaaac agtttgattg atgcggtttc agacggcatt ttcatttcat atgccgtctg
    12601 aaagcaggtt ttaattgttg tccgatacgg acgtttgtag aaagcattga acaaggcatc
    12661 tgtggatatt gattcatgca gatgccgtct gaaaataacc cctatcaatg gagtatcaaa
    12721 ccatgagcaa caacatcccg gccgaactga aatacgttgc cagccatgaa tggctgcgcc
    12781 ttgaagaaga cggtacgatt accgtcggca ttacccacca cgcgcaagag ctgttgggcg
    12841 acatcgtgtt cgtcgagctg cccgaagtcg gcgcgaacct tgccgctgaa gagcaagccg
    12901 gtgtggttga gtctgtaaaa gccgcgtccg acgtgtacgc accgattgca ggcgaagtcg
    12961 ttgccgtcaa cgaagatttg ccaagcgctc cggaaactgc caacagcgat ccttacggtg
    13021 caggctggtt cttcaaactc aaaccggcaa acgttgccga ttacgacagt ctgctgactg
    13081 ccgaacaata cgcgggcgaa gtggattaaa ccgcccggct gcccgacggc aaccgccgga
    13141 caaacggaaa ctgcaccttc agacggcatt tttgcggtcg gaggtgcagt tttttgtccg
    13201 tgttttaagg aagcagttag gctataataa cggtctatat tcatctttac cgattttttc
    13261 atgcaactta ccgctgtcgg actcaatcat caaaccgcac ctttaagcat acgggaaaag
    13321 ctggcgtttg ccgccgccgc cctgcctaaa gccgtccgca atcttgcccg aagcaatgcg
    13381 gcaacggagg cggtaatcct ttctacctgc aaccgcaccg agctttactg cgtcggtgat
    13441 tcggaagaaa tcatccgatg gcttgccgat taccacagtt tgccgattga agaaatccgt
    13501 ccgtatctgt acgcgctgga tatgcaggag actgtgcgcc atgctttccg cgtcgcctgc
    13561 gggctggatt cgatggtgtt gggcgagccg cagattttag gacagattaa ggatgccgtt
    13621 agggttgctc aagagcagga aagtatgggt aagaaactca atgccctgtt ccaaaaaacc
    13681 ttttccgttg ctaaagaggt ccgtaccgat actgccgtcg gcgaaaactc ggtttccatg
    13741 gcttccgctt ccgtcaaatt ggcggaacag atttttcccg acatcggcga tttgaatgtc
    13801 ttgtttatcg gcgcaggcga aatgattgag ctggttgcca cttattttgc cgccaaaagt
    13861 ccccggctga tgacggttgc caaccggacg ctggcgcgtg cacaggagtt gtgcgacaag
    13921 ctcggtgtca acgccgaacc gtgcctgctg tccgatctgc ctgccattct gcacgattac
    13981 gacgtagtgg tttcttcaac ggcaagccag ttgcccattg tcggcaaagg catggtggag
    14041 cgtgcattga aacaaaggca gagtatgccg ttgttcatgc ttgatttggc agtgccgcgt
    14101 gacattgaag cggaagtcgg cgatttgaat gatgcctatc tttatacggt ggacgatatg
    14161 gtcaatatcg tccaaagcgg caaggaggca aggcagaagg ccgccgccgc cgccgaaacg
    14221 ctggtgtccg agaaagttgc cgaatttgtc aggcagcagc agggcaggca gagtgtcccc
    14281 ttgattaagg cgttgcggga cgagggcgag aaagcgcgca aacaggtgtt ggaaaatgcc
    14341 atgaaacagc ttgccaaagg cgcaacggca gaagaggttt tggaacggct gtccgtccaa
    14401 ctgaccaaca agctgctgca ttcgccgacc caaaccttga ataaggcggg ggaagaagat
    14461 aaagatttgg ttcatgccgt cgcgcagatt tatcatttgg acaaataacg gtgcgccggg
    14521 aaaaatgccg tctgaagagg tttcagacgg catttttttg tgccgcctga caacatcgtg
    14581 aaatcccaca ttatatcgat gtaatcacaa a
//
LOCUS       DAWISC010000035        14409 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWISC010000035 DAWISC010000000
VERSION     DAWISC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.035"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(143..2116)
                     /locus_tag="WIW87_000748"
     CDS             complement(143..2116)
                     /locus_tag="WIW87_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ5173939.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2127..2687)
                     /locus_tag="WIW87_000749"
     CDS             complement(2127..2687)
                     /locus_tag="WIW87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ5173940.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2684..3295)
                     /locus_tag="WIW87_000750"
     CDS             complement(2684..3295)
                     /locus_tag="WIW87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ5173941.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            complement(3295..3738)
                     /locus_tag="WIW87_000751"
     CDS             complement(3295..3738)
                     /locus_tag="WIW87_000751"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ5173942.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            complement(3752..4099)
                     /locus_tag="WIW87_000752"
     CDS             complement(3752..4099)
                     /locus_tag="WIW87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ5173943.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRMDTA
                     AGGFDYRHEVPVI"
     gene            complement(4200..4868)
                     /locus_tag="WIW87_000753"
     CDS             complement(4200..4868)
                     /locus_tag="WIW87_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ5173944.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(4865..6010)
                     /locus_tag="WIW87_000754"
     CDS             complement(4865..6010)
                     /locus_tag="WIW87_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ5173945.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALGKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGLRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(6000..7331)
                     /locus_tag="WIW87_000755"
     CDS             complement(6000..7331)
                     /locus_tag="WIW87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ5173946.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIANIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7331..9502)
                     /locus_tag="WIW87_000756"
     CDS             complement(7331..9502)
                     /locus_tag="WIW87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ5173947.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTGLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLS
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQNYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9657..10259
                     /locus_tag="WIW87_000757"
     CDS             9657..10259
                     /locus_tag="WIW87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173948.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(10303..10536)
                     /locus_tag="WIW87_000758"
     CDS             complement(10303..10536)
                     /locus_tag="WIW87_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173949.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10511..10876)
                     /locus_tag="WIW87_000759"
     CDS             complement(10511..10876)
                     /locus_tag="WIW87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173950.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(10880..11257)
                     /locus_tag="WIW87_000760"
     CDS             complement(10880..11257)
                     /locus_tag="WIW87_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ5173951.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11322..12731)
                     /locus_tag="WIW87_000761"
     CDS             complement(11322..12731)
                     /locus_tag="WIW87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ5173952.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12734..12931)
                     /locus_tag="WIW87_000762"
     CDS             complement(12734..12931)
                     /locus_tag="WIW87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ5173953.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(12928..13578)
                     /locus_tag="WIW87_000763"
     CDS             complement(12928..13578)
                     /locus_tag="WIW87_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ5173954.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13568..13990)
                     /locus_tag="WIW87_000764"
     CDS             complement(13568..13990)
                     /locus_tag="WIW87_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ5173955.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(13990..14388)
                     /locus_tag="WIW87_000765"
     CDS             complement(13990..14388)
                     /locus_tag="WIW87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173956.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         2803 a         4188 c         3899 g         3519 t
ORIGIN      
        1 cccatctcta cagcccatct ctacagccat ctctacagcc atctctacag ccatctctac
       61 agccatctct acaacccatc tctacaaccc gtctctacaa cccgtctcta cagcccgtct
      121 ctacagccat ctctacagcc cgctagtctt gcagtgcctc gagcaagccg tctcggcggc
      181 ggcggtttcg gggtccggtt gatgttcttc cactctctct ttgcggttct tcgttggttt
      241 caggtatttc ggggcctatc ggggagctgg cggcgtttcc cgaggcggct ttgccgatgg
      301 ccatccagtc gcaactgcct tcgtaccagt tggtgataat ggcggcggcg tggttttttg
      361 cgcggataaa cagccagttg tgttgccttc gatcgccggt aacgcgttcc gaatggcgtt
      421 cggatacgaa gcatttgacg ttgccgtcgg caaaggcgac ggggaagacg acttcgtttt
      481 cgatggggcc gccgcttcgt gtgaacctgt agctgccggt ctgtatcatt gtgccgtcgg
      541 ggaatctgac aatatccacg ccgccgatgg tttggcgtgt gaattgcgcg gcaacggcgg
      601 tgctgatttt ttcgtccagc ccttggattt gggcggcggt gtgggtgtgg tctcggtcgg
      661 ctttgtcttg caaaccttgc gccagcgtgc ccgcgtccag cgcgcctgcg ttgatggttt
      721 cgccgtggga ctttatccaa aacacggctt cgcccaaggt gtcggcggct ttgatgcaca
      781 gttttaaaac cagggctttg gggcggtttt cgtcgccgcc tgtcgccatt ttgctgtccg
      841 agcgcggggt taaaaagccg ttgtcgttta aattttcgtc cgtccaagtc gatacgagcg
      901 cgcttctctg cctttcgttg cggtcttcgt aaccgagggc tgccgcgtct gtgtggtttg
      961 tccagtgtga aaatactttg tggacgtgcc gtttgatttc gtcttcctgc ttcgttccga
     1021 ctgccaagct gttgcccgcg ttgcggataa agcggtcttc cgcctgcggg acgttttgga
     1081 tgctgccgta ttttccggtc agcagacggt acagctcggg ataagcggtt tcggttacgc
     1141 gcgtgcggat gtcgtcaaac gccagccagc cggtcgggat ttggtcggac ggaaaccacg
     1201 cggtgatgcc gatgtcggta cggcttaaat cgggcaggcg gttgctgttg cccaaggcgc
     1261 ggtaaaggtc ggggaaggtg ttttgtgcaa aggtcgtgcc gttggctttg agatagcctg
     1321 cggggttttg tacggctttg ggaaaggata cgatcgcgcc gacggggatg ccgtccgaat
     1381 tgagtttttt ccatgcggac cagttgtcgt ttgcgctgct cgtttggtgg cgttcgtaca
     1441 cgtcggacgt gtaggcggga taacccaact gcctgcacca gccgtgtttc gtgccggcga
     1501 tgacttggat atggcaggcg gtgttttcaa cgggcaggtt ggagctgccg accgccgtcg
     1561 gcagggaata gatgccgtct gttttgaggg tgttcaaatc gccccggaat gtttcgactt
     1621 tgaaattgcc gatgccgtat ccggacagtg tgttcggttt gccttggatg tcttcggcaa
     1681 aacgcggttt gttggctttg tggaagatgt cgtggttgtt gtaggaaatg cggttgtttg
     1741 cgccgtgcag gcggagggtg tcgtcgccga agacgatgtg ggcgttgtcg gcatccgcgc
     1801 cgatgtaggc tttgtggccg ccgttccagt tcgtctggtt ggcggataaa tggatgccgc
     1861 tttggaattg ggtttgggcg gtaaaggttt tttggccgtt gacggtttgg tcgccggtga
     1921 taactacagc cagcctattt agtgctgcca gtaattgatt aggctgcgac ttatccggct
     1981 ggataccggc cgcagtcagc acggccagca gctcggattg cacttggttg agccaccacg
     2041 cgggcaggat ggtgcccaat tcggacacgc cgtcgccgtc gtggaaggtc ttatcggggg
     2101 tttcgatggg gtgcataaat cgtcctttag cggtaggtaa atcggatagc ggtgtgggcg
     2161 ggcttgaggc ggttgaacag gctctcgatc acggcatcgc tgtaatcggt cagcctgtcg
     2221 cccgccgccg agataccggc gcggaatcgg gtaatgcggt tgttgccgcc gcgcacgtta
     2281 acgtgccaca cccacatgat ttcctgcggc gcaagacggt cgccggcgcg gtttacaccg
     2341 gcgcggaacg gctgcggttc gtcgatttgg atttgatagc ccgccgcctc ggccaaacgc
     2401 acaaaataag gaatactcaa gccgcctgtt tcgtttagct tggccatgac ggccaacaca
     2461 cggtgctggc ggtttttgcc cgtaccgtcc aaacctaata cgcgctccca atcggccagc
     2521 atttggccgg cgctgcgcgg gtcgacggca tcggcaacgc tttgagccga ttccgccacc
     2581 gcatccagcg ctgcgccgtc tatttctgcc tgcgcccgca cacgcggggc attgcgggca
     2641 tacgacacgg ggggcaacag gccccgcaag atgtcttgat agctcatgac ggcatctccg
     2701 ttacattaat cgcgcccgct ttaaaccact cgatgcggtt aaccgtatcg gcggcacggt
     2761 tggccgtcgg cgcagtcaga cggcggtcga tcacaccatc cacattgctg atagcagcct
     2821 cgatttgcga cacagtcagg ccgtcgccgg ggatcagggt gtcgaaatat tcttttaggg
     2881 ccgtccgtat gcggcgcttg gcctcgtcca agtcgatacc gtcgagcttg acttgcacgg
     2941 taacaggcac cgccgttacg gttggcttga gtaccagcgc attttttgcc gttaccgggc
     3001 gcatctcgtc gatataagcc tgtacgcggc gcacagtttc ttccgacgac acaccgtcgg
     3061 cggaggtaat ggcaatatcc accgtaccca agccgcggcg cagcggataa acatatgcgc
     3121 tggttacgcc gtcaacactc aacgcccagt ttttatagtc gtaacggttg ccgcctgcgg
     3181 gcggtcggcg gatgatttcc aacagacgcg ccagcagtga ggcatcgctt tctcggtcgg
     3241 tgccgccttg tacggtaagg cggcattcgg tggccacacc ggcgggggcg gccatcagtt
     3301 gcgcctcgcc gtcgccacat tggcggccgc gcccggctcg tcggcgattg ccggtatttc
     3361 cgccgtgccg ccgctgccga taacggcgcg ggcggtagtg cggtaaaaac gctggccgat
     3421 acgcacttgc aggtcgtctg aaagcatgga ttgcgcaata ccgcttacgg tcagcgtgcc
     3481 gctggccgtg gtaggattgc ggcggctcaa gccgcgcatg gaggcatgcc gctccaaata
     3541 ctcgcggtcg gcggtatcag ggaaaatctg ccgcacaatc cagctttgat gcgcatattg
     3601 cccttcggcg cagctggcca aacggctggc gtgcacataa tggtcgctgt cggggctgat
     3661 atcggcatcc ggccacaggc ttttggtatc gcgcaggata cgctcgcgga tttgctcaaa
     3721 tgtcggcgtt tcaaacacgt ttaaaacctc tttaaatcac gggcacttcg tggcggtaat
     3781 caaacccgcc cgccgccgta tccatccgga tatgcaggat caggcggccg tcgtgcggct
     3841 gctctgcgcg cacggtaatc ttgtcggcac gcccgctctt aacaatcggt tgcagtgcct
     3901 catcggcata ttgctcggcc aacaggctga cccgttgcag gtctttttcg cgctgcaaca
     3961 aatgcagcag cgagccgagc gttttatccg cccaccagct gcccaacggt gtcatcaagc
     4021 ggatatacac ggcattttgc agcgtatcga cggtgcggcc ggtatagtcg ccgatgctgg
     4081 ggtttagctc tttgtccatg ttggcatttt gccgttttca gacggcctca cggagtacac
     4141 gggcttcaat cccacacgca caaaaaaagc agcccgaagg ctgccaaagg tctgcttgtc
     4201 tatgccggtt ccgccggtaa ggttttgccg ccgatgctgt cggtatgcgg gtgctggcgc
     4261 aacgatatat tgccggccac cacgtcgccg tcggtgttaa agctgccgcc cgtttggtta
     4321 acatcgccgc taaaggtggc tccgtcgccg ccctcgacgg ccatgccgcc gttgccgttg
     4381 atttggcctt gcgccgtcaa cactgcactg gtctccacca acggagcgtt aaatttggca
     4441 tccgtggccg cattaacctc gtattgtttg cagttaaccc ggtacacgtc gcaatcggcc
     4501 tcaatgattt tgccttgctt aatcacgatt tttgcaccct catgattaaa aatcgccgtc
     4561 tcgccgggct taaggttttt gatgcggtag ctgccgtgct ggctgcacac aatcacaccg
     4621 tgcgaagtat tgccgcccag cggtatcact accgcttcgc tgccgtcggg cggatggctg
     4681 gcaaagccgt attcctgcaa atgttcaagg tcttgcaggg tttcgtcggc caagccgctc
     4741 aactgcacgc gctgtatcgg ctcggacgac accaccagcg tgatttttcc ccgaaaggcc
     4801 gcgcgcaggg tttcgccgat atttttagca gtttgtgccg tttttttcgc caatttactc
     4861 aaactcattc aaacaccgcc gtttctgctt gttttttgcc gccttttttg cccttatggc
     4921 tcacgccttt gcgtttgcct ttgcgcttgc gcgccgcctc ggcctttttg gggtaagcgt
     4981 cgggtgtcca aataccgtcc tctttgagcc gcagctcggt ttgcgtacca tccatgcggg
     5041 atagcataaa ccgccgcccc atcagaaaaa acaccgcatc gataccgtgc tcgtcgtcga
     5101 tcacatgcac acgcaggcca ggttgccaca atacgccgtc gcgggtttta tggccgccca
     5161 cggttatcgt gagtgtaaat ccctccagcc gccagtcggc cagctgcttt ttagcctgct
     5221 tttgcaatgc ggccaaattg tcggcatcgg acaccaccac cgttttaggg cggtgcagcg
     5281 tcatcgtcgg gtctttgtac acccacttta aatcgtgttt ggcgctgtcg ccgctgcggc
     5341 cgtgcgattg cgccaaaaaa gtaacctcgg aaaagcggtt gtcggtatcc cactcaatgt
     5401 ccatgcgctc gatattgcag cggctgtcgg tgcggctcca acacaatgtc gccaccggcg
     5461 ggctgctgta atccgcaccg cccaccacca acgtgccgtc cggctccagc cacggatgca
     5521 gcccgaccga gttggcaata tgggttaatg cctgccatac ggtttcgccc ggctcgatgt
     5581 cgattttgcc caaagcgggg ttgttttcgg ccttaagcac caccgcttta atctgcggcc
     5641 acggcgcggc cagctttttg gctgcatcca ataccgtcat gccctttaca ttgagctgcg
     5701 gcgcggagca atccaccaaa aaaccggcca aatcacgccc gctcaagctc aactcgcggc
     5761 tgcccttgct tttgccgtgg cgctggctgc cgatgatgcc cgtcatcacg atttgcccgt
     5821 ctatcactac ctcgcagctc tctccgctta aatcgggtat ggccgcctcc ggtcccaacc
     5881 tgccgatgac aaaatcgaag ctgtcggcag ggattaaaaa gtcgctgtcg atgtcgtagc
     5941 gctcccagtg gcggtgctct ttaccgccca cgcgcaccga cacggcatag ccgtatgaat
     6001 tattttgcat agctgttgac caaagtgccg cgcttgataa acgcggggtg gtggatatgg
     6061 ggattgagcc gcaccagctc tgctgcgcgg gctatatcgc cgtaaaactc gtgggcgatt
     6121 tggtgtatcg taccgtcgat tggggcttgg cgcacaatca gcggcggctt ttggttgatg
     6181 accgccgcaa ccaacgcatt cagacggcct gccgccgcgc gcagggattc tgccgtttgg
     6241 taagcctcgg tatacacggc gttggccgtc agcccaccag actcggcagc agccgtctgc
     6301 accgcccgca aggcggcaat ctcggcctgc atacggcggc gcatggcgcg gttaacctca
     6361 atcaaatcgg gcgcggtcat ctcttcgcca tgcgcctcga ttaatgccgt tgccaccgac
     6421 aacagcgatg acgtggataa caggcgcacc gcctgcgcta ccggctgcac ctgtttggcg
     6481 gttaaccggt tcaggcggtt ttgcaggccg tctgaaaagc ggccggtcag caaattatca
     6541 gggatagcgg cggcgcggtc ggcaacagcc gcagccccgt caaaccgctg tcgcggcgac
     6601 caaccggcat ggtgcatggc attatcggcc aaacccgcct cacggcgtat gccagtatct
     6661 accatgaccg atatatccgc aaacagcttg gccgagccgt ttttaaacgc cgctgcactg
     6721 tatccgcccc gatcgggaaa ggcaattttg tccaaatcaa acaaacggcg caccgcgcca
     6781 aacgtacccg atgccgcact ccaaatgccc agcgcgctgc cccataaagc tgatacgccc
     6841 gcatccaccg ccaacaccgc atcaacaaag ccgatagccg cctcgcggta ggtgtcgata
     6901 ttagcaatca acgcctcaag ctcgaccaaa aaggcgtttt caaaaacaaa gatttcctgc
     6961 gcttcggccg cctcgcggaa agtaatatcg atgcccgcat aatccacata atcggcctca
     7021 tgtcggtaac tccatgatgc cgcaatcatg ttgtgcatcc gcccccaaac agggtgcacc
     7081 agcacgccgc cgcccggctg ctccagcgca tccagcagct ttttcagacg gcctgcatag
     7141 cccttgcccc aaaacaccgc attaatctgc acctgccgcc cggtcatgcc catgtcttca
     7201 aggtcgatac cctgcacaaa cggccgcgca tgctcggcca atgccttgcc gttgctctcg
     7261 tccaccacct caatatcaaa gccgacgccc ttgtaagatg cgtcctgcaa taaggtatgc
     7321 catccgctca ttattgaccc gctccacggc cgaacatggc cacttgatac cgcgatacct
     7381 cattcgcgat aacacgtccg tcgatattca ggctcacatt ggtattgatg gtctgacttg
     7441 ccgacgccat cgcactttgc acttgtgcca aacctgttgt aactgcagcc gtatcctgcg
     7501 ccaatgctgc ctgatacgcc gccgtctgct gctgaatggc tgcctgatac gccgccgtct
     7561 gagttgaaaa caacggagac aacatcttat cggtaggctg cgctgacgga gcagccaaat
     7621 actgagggac accaccccgc gcctgttccc gcttcggcgc aggttcaaat cggataccgt
     7681 tgtttttcag acgattaatc atttcaggag ttgtcttggt accacgcaaa ccctttggca
     7741 atgtgccgtc acccaaagac tcggaataca gcaacatacc taacaaaccc gcccgtttaa
     7801 ctaacgctgg attattcatg agcccgctac cggcagattt tccccatgac agcaacttac
     7861 cgcctgccgc tgtggcacct gctgccacgc cgcctgcgga agccttaccc catcccaacg
     7921 ctttactacc tacatctttc agaaagcctg cacctttacc gccaccggcg gttaataaca
     7981 ttgcggcaga cgccgctgtt gccgccgtcg tcaatgcttg cagggctaga gtggcattag
     8041 ggtattccgc tgccagcttt ttaaacgagg tttccatatc caccaatgtt cccgtcagac
     8101 tttcccgcaa cattgccagc gattcctgtt gctcttgttg cgccgctgcc gacaacattc
     8161 gcgcctcgtt gttcttagca atttttccgt tatccaacgt tacgccagcc aacgaagcca
     8221 tatattcacg gatttgcgtc atatccgttg cagccagcaa accttgtttt gcctgcaaat
     8281 caggcagcag ttgcgccaaa agcgcgccct taagcatatt tgcctgctcc gccgccgtct
     8341 tatcgcctgc agccgcgcgt ttcttataat tttggtattg cttatccttt actagcatgg
     8401 catcggcaag acgggacaac acctgcactg cgttttcgcc gttttgcttg ccttgcacaa
     8461 ccgagcctat ccaatcgaca cctttcttcg ggtcattcgg atttgccatc ttcttcagac
     8521 gacctatcgt gtcaggcgac agagttttac tcaaaagatt ttgcacatta gtcgccgctt
     8581 cggcaggact gcccgattta ttcgccgcag attgtaagag tgagagcagg tagtccaaac
     8641 cgccgacacc attcatccct gcctgttgcg cggcagagag caggctcggc agctcccgaa
     8701 ccatatcccg cacctcgaaa gtgccgtcta aacccgattg caagacgtgc tcaagcccga
     8761 gttgcaggtc tttaccgctc atgccgccat ctttcagggt tttaatcagt tttgccgtat
     8821 cctcgccact gccttctgag gcaagtgcaa aagcatatgc cgcctgcgct tcattcttgg
     8881 tttgggcaaa attcagaccg gtggtcatca tgccgctgat taaatccaaa gccttatcgt
     8941 gggtcccgcc atttttctcg acaagttcaa gtgccaaatc cttgatctgt tgcgcacctt
     9001 cagttgcaat ccacgctgcc gatttactgt tatcctcaat aaatgcctgt ctggacacgc
     9061 ggttgatgtt ctcatcaagc tgctttctgt tgtccatagc aggcttaagc accgtatacg
     9121 ctgccgcgcc accagccacc gcagctgcac cgaatcttcc gatttttccc atcttgtccg
     9181 caaatcccgt gccctgtttc agttccgcgt tcagctcgcg aattcggtta cgcgtagcga
     9241 ccgccgcccg tgccaaatca ttttgtgatg ccttgccgct gcgcgccaaa cggttaaacg
     9301 cagcctgtgt cagcatgatt tcgcgttgaa tctgtttttc tgagcggatg ccggtcaacg
     9361 cataagtatg gctttgccgt tcaagttgcg cccgcgattt cgcggtacga tctaattgtc
     9421 gttgagtatc agccagtagc cgtctcgctc cgtcatctcg ggcggttaac accaacgaca
     9481 gtttcatgtt cccgtttgcc ataaaaagca gcctttaaac gtattaactc aaggtcaatt
     9541 atcgtttaaa ggctgtttaa gggaggcggt atgggcttca accccgtttt cgtgctttat
     9601 ttgtttgttg cgttataata ctagaatgat aaccatttta ttcaaaggga tattttatga
     9661 atatgaaaaa attgatttcc gcaatttgtg tttcaattgt tttatcagcc tgcaaccaac
     9721 aatcaaaaac ggcacaagcc gaagaacctg tccaaagtat ccaggctgct gattgtaccg
     9781 ccccaatgga catcacagtt gaacaatatc tcatcaattt ggagcaagca tttaaaactc
     9841 agaacgtctc aacaaaaatc cataataaaa atattgtcaa gaccgattgt ggttatgacc
     9901 ttactttggt aatggatttt ggggcgattg cgctcaaact ggacgagcag caaaaaatta
     9961 gagctatctc agtaggctac attttaaaaa ccgacggaga gaaaggacaa aatctagtca
    10021 ataatgccat aaatggatta cacagtattc aggcagttct gtctttaact accacagaca
    10081 aattgggcga atcggaagca ggaaaacaac tttttacagc tttaaccgaa gtcgtcaaag
    10141 aatccaatca gacaggagca acagcgcaaa aagacgttcc ggcagatggt attttatata
    10201 gcgttgtttt tgaaaaagaa acaaacacca ttgcaataat cggcagaaaa caaccctaac
    10261 cgtcaaataa aaaaaaggtc gtctgaaact cagacgacct ttttattttc cctccggctt
    10321 tctcagccgt ttcgacacaa tcactccctc ttccttcggc gtttttatgc ccaggctcga
    10381 caggatgtct tcaaaccatg ccgacagctc ggcgtggcac atatcgcgca ctgcgtccgc
    10441 ccctatcccc gccttgccat caggatgacc gcctgacggt agcttttgtg tgccgtcagg
    10501 tagctgcgtt ttactgctcc gccgccgacg gcgcatcggg gctcgccgta gcggcggacg
    10561 gttttaggcg caaatcctcc tgagccgcca tcacggtatg gtagtcttcg gtagagaggt
    10621 tttccatcag ccactgcgcc gtcaggatgt cggatgggat accttgggct tcaatctgct
    10681 gcgaccagta ggtcagcgtc tctaggacgt cgcgttgcag cagttcggct tttgtggcgt
    10741 tttcgggcag ctcgggcaag tcatcaatca gggcgaacgc cgccagttcg ccgccgacgg
    10801 tcagtggctt gaggcgtaat tcagatacga ctttgccatt gtaggtcagc ccgtgggtca
    10861 gtttgacggt aatcacgcat tactccttga ttttgtgcaa agcaatcatc tcaatgtcga
    10921 tgcgtgcctc gttatcgact tcatattgct cgccggtatc gacggtaaag cagtcgaggt
    10981 aggaagtgcg gcgatcttcg tcgttgatgg ggtaaatcgt gattttcgcc ttggtgatgt
    11041 tgtcccagtc gatttccgta ccgtcgatcg gaatggcggc ggtaacgctt aatttgtgtt
    11101 cggttacgcc gtcacaataa ccgttgactc ggccgttgcg gttcatcgtt ttgaccggct
    11161 tgcggcctgt agtggtttgc ggcttgatgc tcacaatctc gatatcgcgg ccgttcatct
    11221 ccataatcac cgcgtcggca taggtagcgt cgctcatgct ttatcctttt ttaaggtttc
    11281 agacggcctt taaacctgtt ttaaacgccg tctgaaaagg gttacaaaat caaatcaatg
    11341 cgcccggcaa agacgtgcag gccgttgacc acatcggcgg ggataatggc attaacacgg
    11401 ttggggtcgt tttgcgcacg cgccaccacc agcttgcctt tgttggcctc ggcgttttcg
    11461 atgatttcgg cttggtcgag cttaatcagc acgtccaaaa tctcgctctt aaccttgggc
    11521 agcaggcggt cgctcaattt gtcgcgcgga aaacgcaggg caatgcgctc tttaacgctg
    11581 cggcgcacat aatccagcgt gcggatggtg gtaatgtcga gtagtgccgg gtcgtcggtg
    11641 ttgttggccg acttggtata ggtggatacg gcacgcataa tctgcacgcg gttgttgacc
    11701 actgtgagcg gggtcaagcc gttgtacagc gcattgttgc attctgcaaa cagcggccat
    11761 tgcgcatcag gtgtaacggc cagccctttg atttccagcg tgttcagcgg cttggcaggg
    11821 tcttcttcaa aggccaacac cgccgcataa cctgcggcga tgatgccgtt tggctctacc
    11881 gcacctttgt accaagcaca ggtcatgcgg ccgtcgttga tttcgccggt agcggtggtg
    11941 gccgtgctca aggccgcact catacccaat acgccgatac agccgcgctg ctcgatggcg
    12001 ttggatacgt tggtaatatg gttgctcaag gctttggcgt tggcgtcatc gctaaacggg
    12061 ctgcaaataa tgtgataatg cttgcccgcc actttggaca aggccgtggc aatgtccgca
    12121 tttttggcac cgccggtaaa ggcattggct tgataagtta ggccggtatt gccggtgctc
    12181 acggttaagc cgctctcgtt gccgatctcg cctttgtgtt tggccgtcag cgtaacggct
    12241 gcgccgctgc cggatgcggt aacggttaca tcggcggcag tgatggcggt tttcagacgg
    12301 tctgccactg tggcggcggt ctcgccggtg ttaacggccg tgcttacctg cttgccgcca
    12361 atcgtgattt ccaccacgcc cggcgcggtg gccgtgccgg aaagggtaac ggttgcggtt
    12421 gcctgcacgc ctgcgctgtg gtcggcaata ccgataacgg tcaaatccaa ataagggttg
    12481 ttggcaaatg cttggcgcac catcaaatgc gccagcgagc cttgtccgaa caaatcggcc
    12541 gcctcggcat cgctaaatag ttgcaccggc tctaaggcgg gctgtatgcc cgcggtcagc
    12601 atgggtgcaa ccatcaatac cttttgcgga ttttgcggca aaccttgtac ggcattgcgg
    12661 gtgttaaatt caatatactg cccgggcacg cggatgctgc ccggaatcgt gtcaaaatca
    12721 atatggggca tcattactct cccttagtat tgcgggtttt ggtatttggg gcggcatcct
    12781 caaccaccac caaatcgccg tcatcaatca tgcggcggta atacaggctg ttgccgtcca
    12841 cctccaccgg ctcttggccg atatattcgt gcgggttgtg ttcggtcggc acacgcaggc
    12901 catctgccgc cgttactttg attttgctca ttgcttatcc ttaagggtta ggtttacagg
    12961 tatttcatcg gcggattgcg ggtcgtaaat tttgccgtcc aacccctcga aatcaggcca
    13021 cggctcgctc aatgtaccct gatacttggt aaagatatgg ttggggtcgt cgggattgtc
    13081 ggtgcgttcg gggtagcggt cattttccaa cccgcaggtg ttaaagcgga tggcatactc
    13141 aaccgcatat atgcttactg cggcgttttg caccagcaca tgattggcaa tcgcgcgcac
    13201 cgctttgggc accaagccgc ggctatcggc aaaaccgagc cgctggccgt caagcaggcg
    13261 gcgaacagcg cggattaaat cgttgctgcc gatttcgcgg ctgtcgatgc cgccttgccg
    13321 ctgcgcctgc tcgttgcgca gattgcgggc cgccaccatc accacaaatt cggcggtatc
    13381 ctgataacgt ccgcatacgc cgccggtgct ggcaggctca actttgctgc cgccatacgt
    13441 tacccaaacc gcaggcagcg tatggatttg ccccgccaaa tcgtcggcct cgccgttgta
    13501 gcttttaacc gtgcgcacca tgcgacccaa gccccgtttc agacggtctg ttatcgcttg
    13561 ctcaatatca gtgatcacgc ccgaacacct tatttttacc gtttacaaac acaataccgt
    13621 cttcgcccgc ggccacttct tcgccgctat catccacgcc cagctgcact tcgcctttgg
    13681 ccatagcacg cagcaggtcg agcacgtcga ttttgtagcg gttgcggatt tcgtcggtaa
    13741 tcaacacgcc ctgagccgcc gtcagacggt agcgggcaat gtcgcagcaa aggcgcacca
    13801 agatgggcgg cagatcctca aaaggtcgtc tgaaccgccc cagatacgcg tcgatttcgg
    13861 cagtggcgtc caccagcgcg gtttgtgcga cctcgcggtc aatcagcccc tcgttgttgc
    13921 ggtcggtgag ctgcaagact tccagctcac cgaaacgcgc aaccatatcc tcaaccgtcg
    13981 cgtatgccat tactcgaccg ccttgcgttg cagcataggc tcggcgcaga ttgccttcca
    14041 caccgcttcg ccgacttcgg cgcgcttcac ttcgcgccag ccgccgtcaa acagcaggcc
    14101 gccgcgccaa aattctttgc cgtctgcgcc ggtactgacg agcatcacat cgcggctgtc
    14161 cgccaaagcg tcggcggcac gttgcgtatg ctgcactttg agttcggcaa gttcggcgga
    14221 cagtgccttt ttgtcgtctt cggctttttc caaggctgtg gtcagcattt cgacatcgtt
    14281 tcgggcggcg gcaagctctg cctgcacggc gtccaattcg gctttgatgt cttcaaacga
    14341 cggggcggcg gtttcggcgg tttctggttt gttgttggtt tttgccatga tgactccttg
    14401 tttcagacg
//
LOCUS       DAWISC010000036        13994 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWISC010000036 DAWISC010000000
VERSION     DAWISC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.036"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            52..1005
                     /locus_tag="WIW87_000766"
     CDS             52..1005
                     /locus_tag="WIW87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ5173957.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WIW87_000767"
     CDS             complement(1116..1439)
                     /locus_tag="WIW87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173958.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WIW87_000768"
     CDS             complement(1612..2973)
                     /locus_tag="WIW87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ5173959.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGTGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAETIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAAHRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHLLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WIW87_000769"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WIW87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ5173960.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIAQLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVGRKEKILIVADYDADGATACAVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNDFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5755)
                     /locus_tag="WIW87_000770"
     CDS             complement(5039..5755)
                     /locus_tag="WIW87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173961.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEEHTDKSKLFANILYTPPFLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(5802..6314)
                     /locus_tag="WIW87_000771"
     CDS             complement(5802..6314)
                     /locus_tag="WIW87_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ5173962.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6461..7792
                     /gene="pmbA"
                     /locus_tag="WIW87_000772"
     CDS             6461..7792
                     /gene="pmbA"
                     /locus_tag="WIW87_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ5173963.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLAHPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTYGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8089..8292)
                     /locus_tag="WIW87_000773"
     CDS             complement(8089..8292)
                     /locus_tag="WIW87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ5173964.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8535..8837
                     /gene="clpS"
                     /locus_tag="WIW87_000774"
     CDS             8535..8837
                     /gene="clpS"
                     /locus_tag="WIW87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ5173965.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8839..11148
                     /gene="clpA"
                     /locus_tag="WIW87_000775"
     CDS             8839..11148
                     /gene="clpA"
                     /locus_tag="WIW87_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ5173966.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDEDEDDGNY
                     SSDGMDDDNGNRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRK
                     NNPLLVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEA
                     RVKSVLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATT
                     YDEYRTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAA
                     AELSARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEK
                     TVSHDDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPT
                     GVGKTEVAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAINK
                     QPHCVLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLS
                     RPSLGFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQLEH
                     RLLDKKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGG
                     FVRIDWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11187..>13994)
                     /locus_tag="WIW87_000776"
     CDS             complement(11187..>13994)
                     /locus_tag="WIW87_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ5173967.1"
                     /translation="PHTGLFLLGKVALQMRIRRRLKNIYLLDKKNLARNHVQVINQFE
                     QTGTHANRYDVTVLVNGLPLVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQ
                     IFVISNGTDTRYFANTTKRDKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVL
                     LHYSVFDANDTLLIMRPYQIAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTL
                     TSFKAARLATESAFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEK
                     DDNKIIVTTIQKLNNLMKGEDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQ
                     FGFTGTPIFPENALGAETTAGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAV
                     EAEQDEKKLSAAENHKALLHPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSV
                     DAAKCYYEAFKTQQAGSLHPLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEF
                     LQAAINDYNACFKTNFGTDSKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLN
                     TLFVDKNLRYHGLMQAFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNV
                     VLEKSYEEYMNGYTDSQTGEARRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEY
                     LRAENVLQNYDEFAALRELQSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAV
                     QDYRSAYNDIRDWLRRQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHH
                     KKIKGKAELVEEIRRIIRASIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQE
                     VMRHEAAGLIAAEGLNETAAKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKK
                     QSVFQKIAAFVEKFAGIGTDI"
BASE COUNT         3241 a         4002 c         3508 g         3243 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgccaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaaa acggctatat ccgcgccctg
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gttgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgc
      541 ccagccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcaggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gccgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgttgcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatgcata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccatg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggcctt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcag atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttt agacactcgc gagcgtgccc tgaaaacagc aatttcataa tttcgtcgaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgt gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccatagg tattgtcttt
     2581 cataatcctg ccgcgtgcat tctgatgtac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gatagtctct gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcttt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgtccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcagag tctgaaattt
     3181 gatgcgcctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggtcgtctg aaacgcggga
     3661 atgttgtgtt caagtatgct caaacccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaggtgcaa attgggaatg
     3781 gaacgtcccg aaccgcgtac ttcgccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcgaca atgccgacca caccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt cggggaaatc attcagtgcg
     3961 tcttgcagca tagactgctc gatttcgcgg cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaagttcgcc caaattcggc
     4321 tctttgatgc cgtctgaaaa ataattgcgg cggcgcaatt cggcacgcaa cgccatcaat
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccgca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtctcggccg gcaagtggtg gtcagtaacg
     4501 atgacatcca aacccagagc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tccaccgtaa tcagcaaatc cacgccctgc gcggcagcga tttcggcaag ttcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acaaggaaat ccactttcgc ccccatcgcc
     4681 gccaaaccgc tcataccgac ggcacacgcc gtcgcaccgt cggcatcata gtcggcaaca
     4741 atcaggattt tttccttgcg cccaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcaacg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaagcaca aagctgggcg attaaaggat cggcaccggc ggtgagcaaa
     4921 tgattaaaaa cgtcggtatt gacggatcgg gtttggattt tgactgacat aacgtggggt
     4981 tttactgtgc gtgtgtgctt aaaatgccgt ctgaaagctt ttcagacggc attgctgttc
     5041 atttgcgggc ggcatccacg accgcaccca gagccgccgc aggcaaggcc agtaccgcgc
     5101 ccgccgcatc cagtatcaaa aaggggggcg tatataagat atttgcaaac agcttggatt
     5161 tgtcggtatg ttcttcagta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 ggtaatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gatacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagtttga ccgcttcaaa cccgagctgt ttcagcttgg
     5341 cgatgtcggc aggcttgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctgccagg cgattcgagt ttgaccggca gggcttggtg gcgagcatag ctcggggtat
     5461 cctcaactat ttggaagggt ttgtccagcc ctgccttcaa aatgcccgtc agcttcgccg
     5521 aatcttcggg attgacgacg aaccagtatt ttccgcccat catcaccagg ctgccctttt
     5581 ccaattgggc attgtcttcg gcaaccacac cgaaggcgcg gatttggtct ttgtcaacgt
     5641 gtttgcgggt gattgtttcg ctgaccgggt tgttcattcc ccacaacatc aacgtacagc
     5701 cgttcagcat caaggttgcc gccaaaacgg cggcggtcgt tttacggaac aacattttat
     5761 cctccaatct aaataacggc ggatgtcgtc tgaaaatatg cctaaatttc cgcgtccccg
     5821 ttttccatca cggttttcaa ctcttgaaac agggcgcgga aattttttgg tggtttgttt
     5881 tgctcctgct cttttttggt attgcggatg agtgtcctca gcttgcccgc gtccgcatgc
     5941 ggaaaatccg acataaactg cgtcaacgcg ccgtcgtctg ccaacagccg tacgcgcgcc
     6001 tgttccacgc gttgcaaaaa ggcgttgtgc gccgcatcgt cgccgcgcag cttggcaaga
     6061 aacgcctcga tgggcgcggg atcggtatcg cgcatcagcc tgccgataaa ttgtgcctgg
     6121 cgtttgagcg cgccgttgga tgtgattttt ttataggcgg ttaccgcctc gtacaaatcc
     6181 gcatccaaac cgattttttt cagcgtatcg tttgagagct tggtcaacgc catacccaaa
     6241 tcctgcaaat cgttcatctg ctttttcatt tgggttttgc tgatccattc gtcttcttgt
     6301 tcaaacatct tgattctcga atttttccaa tagcgcattt taacagaatg ccgccgcgtt
     6361 cagacggcat tttgcaccgc acgccctgca agcggcgcgc ttttgcccct gccgcccgaa
     6421 acggttaaaa tgccgcaaag catcatcaag aaagatttct atgctgttca accacaccgc
     6481 ttccgaactc ctcgacctct gccgccgcac gctcgacttg gcaaaagcga cgggcgcaac
     6541 cgccgccgaa gccgatttca gcgaatcatt gggacaaagc gtcagcgtgc ggctgggcga
     6601 aatcgaacaa atcgagtttc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6661 acgcaaaggc cgcgccggca ccgccgattt ctccgaacaa gccctgcgcg acaccgtccg
     6721 cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgcccaccc
     6781 cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6841 ggaagccgcc gtcggcttgg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6901 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     6961 cacctacggt tttgccgcac accggcaaag cacgcaccac agcatttcct gcagcgtcgt
     7021 tgccgccgac gaaaacggca tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7081 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7141 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7201 cggtcttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7261 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7321 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7381 gccgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7441 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7501 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tgttggtaac
     7561 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7621 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7681 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7741 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7801 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctacccg tttgccgttt
     7861 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7921 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     7981 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8041 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8101 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8161 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8221 gccgccttca tcaggcgtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8281 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8341 tacggcaaac cgagccgtct ggcgttcctt ccagcttgat atactcggcg gatgcccaat
     8401 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8461 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8521 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8581 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8641 ttgtcgtcga aatcctgacc gaaatcttta tgctcggaca agaacaggcg gtagcggtaa
     8701 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8761 aaaccaaaca acaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8821 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     8881 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     8941 atcgaagaag atgcctccgt ccacaacgtc ctcaagctct gcggcgcgga tttgaaagtg
     9001 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9061 ttagacacgg tcgaaacccg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9121 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9181 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9241 gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagatga agatgaagat
     9301 gaagacgatg gcaactattc ttcagacggc atggacgacg ataatggaaa ccgcaccaaa
     9361 ccgggcaaaa accctttatc ggcgtacacc gtcaacctca acgccgaagt caaagccggc
     9421 cgtatcgacc ctttgattgg tcgcaaacac gaaatggaac ggctggtgca aatcctatgc
     9481 cgccgccgca aaaacaatcc gcttttggtc ggcgaagcgg gcgtgggcaa aaccgcgctg
     9541 gcggaaggtt tggcacatca aatcgtcaac ggcggcattc cagacgcgct taaagatgcc
     9601 gaagtgtacg cgctggatat gggctcgctg ttggcgggca cgaaataccg cggcgacttt
     9661 gaagcgcggg tcaaatccgt cttgaaacag ctcgaaaaaa tcccgcacgc cattttgttt
     9721 atcgacgaaa tccacaccat catcggcgcg ggcagcacca gcggcggcac catggacgcg
     9781 tccaacctgc tcaaacccgc gctggcaaaa ggttcgctgc gctgcatcgg cgcgaccacc
     9841 tacgacgaat accgcaccat tttcgacaaa gaccatgcct taagccgccg cttccaaaaa
     9901 atcgacgtgg tcgaacccac cgtttccgaa accgttcaaa tcctgcgcgg cttgaaaccg
     9961 atgtttgaag ccttccacca agtccgctac actcaaggcg cactcgaagc cgccgccgaa
    10021 ctctccgcac gctacatcaa cgagcgtttc ctgcccgaca aagccatcga cgtgatggac
    10081 gaagcaggcg cggcgcaacg gattctgccc aaatccaaac agaaaaaagt catcggcaaa
    10141 gcgcaaatcg aaaccgtcat cgccaaagtc gcgcggattc cagaaaaaac cgtgtcgcac
    10201 gacgacaaac aggtgctgca attcctcggc cgcgatttga aaaacatggt ttacggtcag
    10261 gaaaacgcca tcgacgcgtt ggttgctgcc gtcaaaatgt cgcgttccgg ccttgccctg
    10321 cccgacaaac cgataggcag tttcctcttc tccggtccga ctggcgtcgg caaaaccgaa
    10381 gtcgccaaac agcttgccta ctcgatgggc gtaccgctgc aacgctttga tatgtccgaa
    10441 tatatggaac gccacgccgt atcgcgcctc atcggcgcac caccgggcta cgtcggcttt
    10501 gaacaaggcg gccttttgac cgaagccatc aacaaacagc cgcattgcgt attgctcttg
    10561 gacgaaatcg aaaaagccca ccccgacatt ttcaacgtcc tcctgcaagt catggacgca
    10621 ggcaaactga ccgacaacaa cggcaagagt gccgatttcc gcaacgtcat cctaattatg
    10681 accactaacg caggtgcgga gagtctcagc cgacccagcc tcggctttac cgccaaacgc
    10741 gagcgcggcg acgaaatgca ggctatcaac aagctcttca cgcccgagtt ccgcaaccgc
    10801 ttggatgcga ttatcccgtt tgcgccctta tccgaacccg tcatcaccaa agtcgtggac
    10861 aaattcctgc tccagctcga acaccggctc ctcgacaaaa aagtcgaagc cgaattcacg
    10921 tcggcattgc acaaatatct ggcggaaaaa ggttttgacc cgcaaatggg cgcgcgcccg
    10981 atgcaccgcc tgattcagga aaaaatccgc aaaccgctcg ccgacgaact cctgttcggc
    11041 aaactatccg acggcggctt cgtacggata gactgggatg cggcaaaaga agaagccgtg
    11101 ttgaagttta agaaaagcaa ggtcaaaata aaaaccgcct ccgcataaaa tcaaaaatgc
    11161 cgtctgaaat ttcagacggc attttgtcaa atatcggtcc ctattccggc aaacttctcc
    11221 acaaacgccg caatcttttg aaaaacactt tgtttcttcg tcagatattg cgggttgagc
    11281 ggactcattt tcggcagggt ttcggtcagt tccgtgccgt tttcgctggc atagccgcgt
    11341 ttgagcgagc tgattaaata gcgtttggcg gcggtttcgt tcaggccttc ggcggcaatc
    11401 aatcctgccg cttcgtgccg catcacctct tgcgcgtagg tgtagaaggt ttccagtatg
    11461 gcgggaacgt cgggtacttt gtccaaatcc gtatcgttga tgaaatccac aatcagaccc
    11521 tctttggcgc ggtggccgat gctggcgcgg atgatgcggc ggatttcttc caccagctcc
    11581 gctttgcctt tgattttttt gtggtgttcg aaaaccagtt gcaggatgta atccagattg
    11641 atttcctgtg atttgagcaa atccacctca aaaaccacat cgtcccagtc gatttttgat
    11701 tgctctttct gttcgcctgc tttttggcgg cgcagccagt cgcggatgtc attgtaggcg
    11761 gaacggtagt cctgcaccgc cctttcagac ggcatcggca cttgccgcat ttcctgcacg
    11821 tcttcatcgc tcaggtagta tttttcttga aacgccttca tcgcatcttc gtccgccgcg
    11881 tccacactct gcaactcgcg cagcgcggca aattcatcgt agttctgcaa tacgttttcc
    11941 gcccgcaggt attcgccgaa gagtttggca aaatcttttt tgtctttttc cgtttcgatt
    12001 ttgtcgggat cggggaaacg ctcgcgcaat tcttttgcca catccagata accgcgccgt
    12061 gcttcgccgg tctggctgtc ggtatagccg ttcatgtatt cttcgtaact tttttccagc
    12121 accacgtttt tggtgttttt gtcgccaaac aaggtaatcg catcaatggt tgcctgctcc
    12181 aaatcgcgga agcagacaat attgccgaag gttttggtgg catcgtaaat gcggttggtg
    12241 cgcgaaaacg cctgcatcag gccgtgatag cgcaggtttt tatcgacgaa caacgtgttc
    12301 agcgtcggcg cgtcaaaacc cgtcaaaaac atgccgacca caatcagcaa atctatttcc
    12361 tgatttttca cccgttttgc caaatctcgg tagtagtttt gaaaggcttt gctgtccgtg
    12421 ccgaaattgg ttttgaaaca ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttt
    12481 gcgctgctgt ccatcgcttc cggttcaaaa gtctcatcga caatttcacc gacggcgttt
    12541 tgctcttcgt tggccgcaaa ggaaaaaatg gtggccactt tcagcgggtg caagctgcct
    12601 gcctgttgtg ttttgaacgc ttcgtaatag cacttcgccg catccacgct gctgacggca
    12661 aacatcgcgt taaagccttt gccacccgca ttcagccggt gcgttttctg cctgaactga
    12721 ttcaggatat attgcgtgat ttcgcggatg cgttcagggt gcagcagggc tttgtggttt
    12781 tcggcggcac tcagtttctt ctcgtcctgt tccgcttcca cggctttgaa ctgcgggcgc
    12841 acgtcgttgt aatccacttt gaatttcaat actttttcat cgcggatggc atcggtaatc
    12901 acataagaat gcagctcccg cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt
    12961 tcgggaaaaa tcggcgtgcc ggtaaagccg aactggcaga attttttaaa tttctttttc
    13021 aggttttttt gcgcttcgcc gaattgcgag cggtggcatt cgtcgaaaat aaagacaact
    13081 cgctgatggt aaaccggcag attatcttcg cccttcatca ggttgttcag cttttggatg
    13141 gtggtaacga tgattttgtt gtcgtctttt tccaaattgc gtttcaagcc tgccgtgctt
    13201 tccgaaccgt tcacgctgtc gggcgaaaaa cgttggtatt ccttcatcgt ctgatagtcc
    13261 aaatccttcc tgtccaccac gaagaaaacc ttgtcgataa atgccgattc cgtcgccaga
    13321 cgcgccgcct taaagctggt cagcgttttg ccgctgcccg tggtgtgcca gacatagccg
    13381 ccgctttccg gtttgctcca attcttcgcc tgcgccgagc tgttgatttt ccacaaaatg
    13441 cgttcggcgg cggcaatctg atacggccgc ataatcagca gcgtatcatt cgcatcgaac
    13501 acgctgtaat gcagcaaaac gcccagcaat acgcttttct gcaggaacgt ggcggtaaag
    13561 tctttcaaat ccttaatcgg atgattgtcc gaccgcgccc aattcatcgt gaaatcgaag
    13621 ctgtttttgt cgcgcttggt ggtgttggcg aaatagcgcg tgtccgtgcc gttggaaatc
    13681 acgaagattt gcaggaattt gaacagcgaa ttttcgctgt tgaagctctc tttgctgtaa
    13741 cggtgcacct gattgaatgc ctcgcgcacc gccacaccgc gctttttcaa ttcaatctgc
    13801 accagcggca ggccgtttac caacacggta acgtcatagc ggtttgcatg cgtgcccgtc
    13861 tgctcaaact ggttgataac ctgcacatgg ttgcgggcaa ggtttttctt gtccagcaga
    13921 taaatgtttt tcagacgacg gcggattcgc atttgaagtg caactttccc taacagaaaa
    13981 aggccagtat gcgg
//
LOCUS       DAWISC010000037        13850 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWISC010000037 DAWISC010000000
VERSION     DAWISC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.037"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..1447)
                     /locus_tag="WIW87_000777"
     CDS             complement(122..1447)
                     /locus_tag="WIW87_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ5173968.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1658..2497
                     /locus_tag="WIW87_000778"
     CDS             1658..2497
                     /locus_tag="WIW87_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ5173969.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGETLVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2494..3660
                     /locus_tag="WIW87_000779"
     CDS             2494..3660
                     /locus_tag="WIW87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ5173970.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3924..5189
                     /gene="efeB"
                     /locus_tag="WIW87_000780"
     CDS             3924..5189
                     /gene="efeB"
                     /locus_tag="WIW87_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ5173971.1"
                     /translation="MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5261..5590)
                     /locus_tag="WIW87_000781"
     CDS             complement(5261..5590)
                     /locus_tag="WIW87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ5173972.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WIW87_000782"
     CDS             complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WIW87_000782"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ5173973.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMVGRVIDTAA
                     ALNPQNICVVIGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPNDPTGLGRIIRDSNGSVTAIVEEKDADAV
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKLQLTELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGCKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7090..7362)
                     /locus_tag="WIW87_000783"
     CDS             complement(7090..7362)
                     /locus_tag="WIW87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ5173974.1"
                     /translation="MRKTFLFLTAAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7355..8068)
                     /locus_tag="WIW87_000784"
     CDS             complement(7355..8068)
                     /locus_tag="WIW87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ5173975.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVNALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTPYPPKIRKKSHA"
     gene            8127..9128
                     /locus_tag="WIW87_000785"
     CDS             8127..9128
                     /locus_tag="WIW87_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5173976.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEESAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9222..10070
                     /locus_tag="WIW87_000786"
     CDS             9222..10070
                     /locus_tag="WIW87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ5173977.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10096..10581
                     /locus_tag="WIW87_000787"
     CDS             10096..10581
                     /locus_tag="WIW87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ5173978.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10683..12251)
                     /gene="msrAB"
                     /locus_tag="WIW87_000788"
     CDS             complement(10683..12251)
                     /gene="msrAB"
                     /locus_tag="WIW87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ5173979.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            12397..13662
                     /gene="ftsY"
                     /locus_tag="WIW87_000789"
     CDS             12397..13662
                     /gene="ftsY"
                     /locus_tag="WIW87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ5173980.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESELAQIVENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2945 a         3532 c         4183 g         3190 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagatggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggtaggag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta tccccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgcc
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggcaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacag ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gctgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtatgtgt gccgccggta ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gttcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggtacaa
     1261 cggtatagac ggccccgccg ccgccgaccg tcgttccggc ggggtcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggcgcgg aataggtatt
     1441 ttttcataat ggacaatgtt gccggcagta ataagaaaga tggtttcggg cggcgttgcg
     1501 gcagccgtgg agaggggatt ttaacacagg gcgcagctgc agcctgcgga actttccgcc
     1561 gcgcggtact gcagataaaa ataacttgca tttgtattta caagcaatga aaatattccg
     1621 ataatatatt attcatcatc cttgttcgtt cgcgtttatg ctggtcgctt ttttaattat
     1681 gttgcgcgag ggtattgaag ccgcgctcat tgtcggcatc gttgccggtt ttctgaaaca
     1741 gtccggacat tccaaactga tgcctaaggt ctggttcggg gtcgtccttg cttctttgat
     1801 gtgtttgggg ctggggtacg gcatccattc ggcaacgggc gagattcccc agaagcagca
     1861 ggagttcgtc gtcggcatta tcggtttggt tgccgttgcc atgctgactt atatgatttt
     1921 atggatgaaa aaggcggcgc gttcgatgaa gcggcagctt caggattctg tgcaggcggc
     1981 tttgaaccgt ggcagcggtc aaggatgggc cttggtcggt atggcgtttc ttgccgtggc
     2041 gcgcgaaggt ctggagagtg tttttttcct gcttgccgta ttcaaacaga gcccgacgtg
     2101 gcagatgccg gccggcgcgg tagcgggggt tttggctgcc gccgtgattg gcgcgttgat
     2161 ttatcagggc gggatgcgcc tgaatctggc gaagtttttc cgttggacgg gggcgtttct
     2221 gattgtcgtt gccgccggcc tgcttgccgg ctcgctgcgc gcgctgcatg aggcaggtat
     2281 ttggaacgcg cttcaggaca ttgtgttcga ctcatcaaaa tatttgcacg aagacagtcc
     2341 gttgggcgtg ctgctcggcg gatttttcgg ctataccgac catccgacgc agggcgagac
     2401 cttggtttgg ctgctgtacc ttattcccgt cataacttgg tttttgtgcg gcagcaggcc
     2461 gtctgaaact ttaacccgta aagaggagct gaaatgagaa aattcaattt gaccgcattg
     2521 tccgtgatgc ttgccttagg tttgaccgcg tgccagccgc cggaggcgga gaaagctgcg
     2581 ccggcagcgt ccggtgaggc gcaaaccgcc aacgagggcg gttcggtcag tatcgccgtc
     2641 aacgacaatg cctgcgaacc gatggaactg accgtgccga gcggacaggt tgtgttcaat
     2701 attaaaaaca acagcggccg caagctcgaa tgggaaatcc tgaaaggcgt gatggtggtg
     2761 gacgagcgcg aaaacatcgc ccccggactt tccgataaaa tgaccgtcac cctgttgccg
     2821 ggcgaatacg aaatgacttg cggtcttttg accaatccgc gcggcaagct ggtggtaacc
     2881 gacagcggct ttaaagacac cgccaacgaa gcggatttgg aaaaactgtc ccaaccgctc
     2941 gccgactata aagcctacgt tcaaggcgag gttaaagagc tggtggcgaa aaccaaaact
     3001 tttaccgaag ccgtcaaagc aggcgacatt gaaaaggcga aatccctgtt tgccgacacc
     3061 cgcgtccatt acgaacgcat cgaaccgatt gccgagcttt tcagcgaact cgaccccgtc
     3121 atcgatgcgc gtgaagacga cttcaaagac ggcgcgaaag atgccggatt taccggcttt
     3181 caccgtatcg aatacgccct ttgggtggaa aaagacgtgt ccggcgtgaa ggaaattgca
     3241 gcgaaactga tgaccgatgt cgaagccctg caaaaagaaa tcgacgcatt ggcgtttcct
     3301 ccgggcaagg tggtcggcgg cgcgtccgaa ctgattgaag aagtggcggg cagtaaaatc
     3361 agcggcgaag aagaccggta cagccacacc gatttgagcg acttccaagc caatgtggac
     3421 ggatctaaaa aaatcgtcga tttgttccgt ccgctgatcg aggccaaaaa caaagccttg
     3481 ttggaaaaaa ccgataccaa cttcaaacag gtcaacgaaa ttctggcgaa ataccggact
     3541 aaagacggtt ttgaaaccta cgacaagctg ggcgaagccg accgcaaagc gttacaggcc
     3601 tctattaacg cgcttgccga agaccttgcc caacttcgcg gcatactcgg cttgaaataa
     3661 gccgcaagcg ttcagacggt atttggcggc agataccgtc tgaagtttta tagtggatta
     3721 acaaaaacca gtacggcatt gcctcgcctt gccttgccgt actatttata ctgtctgcgg
     3781 cttcgtcgcc ttgtcctgat ttttgttaat ccactatatc cgccatatat tgcagggcgg
     3841 gatttcaacc tgccgctatc ggttaatgga aaaacggcgt gcagggatac ccatcctgct
     3901 gcacggatat tgaaggaaac accatgagca aaaaacaacc cgcacaaccg accaggcgca
     3961 ctctttttaa aaccgcgatc gcagccggag cagtcggcgc aatcggaggt tatctcggcg
     4021 gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag ccaacactcg ccccaagcct
     4081 atccctgcta cggcgaacat caggcaggca tcgttacgcc gcagcaggcg ttttcgatta
     4141 tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct ggaaaacctg ttccgcacgc
     4201 tgaccgcccg catcgagttt ctcacccaag gcggcgaata ccaagacggc gacgacaaac
     4261 ttccgccagc cggcagcggc attttgggca aagccttcaa ccccgacggg ttgaccgtta
     4321 ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg actcaaagac aaaaaaccga
     4381 ttcatttgca ggaaatgcgc gacttctcca acgataagct gcaaaaaagc tggtgcgacg
     4441 gcgatttgag cctgcaaatc tgtgccttca cccccgaaac ctgccaagcc gccctgcgcg
     4501 acatcatcaa acacaccgtc caaaccgccg ttatccgttg gagtatcgac gggtggcagc
     4561 ccaaatccga acccggcgcg atggcggcgc gcaacctgtt gggcttcagg gacggcacgg
     4621 gcaaccccaa agtttccgat cccaaaactg ccgacgaggt tttgtggacg ggggtggccg
     4681 ccaacagcct cgacgaaccg gagtgggcga aaaacggcag ctatcaggca gtccgcctta
     4741 tccgccactt tgtcgagttt tgggacagga cgccgcttca agagcaaacc gacattttcg
     4801 ggcggcgcaa atacagcggt gcgccgatgg acggcaaaaa agaagccgac caaccggatt
     4861 ttgccaaaga ccccgagggt gatatcacgc ccaaagacag ccatatacgc ctggcgaatc
     4921 cgcgcgatcc cgaattcctc aaaaaacacc gcctcttccg ccgcgcctac agctattcgc
     4981 gcggactcgc ctcaagcgga cagcttgatg tcgggctggt gttcgtctgc tatcaggcaa
     5041 accttgccga cggattcatc ttcgtgcaaa acctcctcaa cggcgaaccg ctggaagaat
     5101 acatcagccc cttcggcggc ggctatttct tcgtcttgcc cggcgtggaa aaaggcggct
     5161 ttttggggca agggctgctg ggcgtataaa tccgccatat aaaaaacgcc gtccgaacct
     5221 tgccaaacgg gttcgggcgg cgtttcttgt ttttgggcgg tcaggctttt ttgacgaatt
     5281 cggattttaa attcatcgcg ctgccgtcga ttttgcagcc gatgttgtga tcgccttctt
     5341 gcaggcgtat gcctttgact tttgtgcctt gtttgatcac catcgagctg ccttttacct
     5401 tgaggtcttt gatgaggatg acggtatcgc cgttttgcag cactgcgccg ttggcatcgc
     5461 gcacttgagc cgcaaggtcg gcggcggatt cggtttcatt ccattcatgg gcgcattcgg
     5521 ggcagatgta ttgtccgccg tcttcatagg tgtattcgga ggcgcattgc gggcatgggg
     5581 gtaatgacat ggtttgccgt ccttatcgga tgtttgtttt ggggtgccgt ctgaaacctg
     5641 aaaccggctt cagacggcat agctttattg tttgtctttt tcaggacgca cccagccttc
     5701 gatgacggtt tggcgggcgc gggcgagggc gagtttgttg tcttcgacat tgcgggtaat
     5761 cgtgctgccc gcgcctgtgg ttactttgtt gccgagggta acgggggcga ctaggacgca
     5821 gtttgaaccg atgcgcactt cgtcgccgat gacggttttg tgtttgtgca cgccgtcgta
     5881 gttggcaata atcgtaccgg cgccgaagtt ggttttgcag ccgacttcgg cgtcgccgat
     5941 gtaggtgagg tggttggctt tggtgccttt gccgatggcg gcgtttttga tttcgacgaa
     6001 gttgccgacg tgtacgtcgt ctgcaaggcg ggcttgcgga cgcaggcggg cgtacgggcc
     6061 gattcggttg ttttcgccga cttcgcagct ttcgaggtgg gagaaggggg cgattttgct
     6121 gtttgcgccg attttggcgt ttttgatgac gcagtttgcg ccgatttcga cgttgtcgcc
     6181 gagctcgatg tcgccttcaa agatacagtt cacatcaatc acgacgtctt gcccgtgttt
     6241 cagacggcct cgtaaatcga aacgtgccgg atcgcgcagg gttacgcctg ctttgagcaa
     6301 ttcttgcgcc tgttcggttt ggaagatgcg ttcgagttcg gtgagctgga gtttgttgtt
     6361 cacgccggcg gcgaggtggg aggcgcgcac ttggacggga tgaactttaa taccgtcggc
     6421 aacggctttg gcgatgaggt cggtcaggta gtattcgcct tgtgcattgt tgctggaaag
     6481 gctgttcagc cagttttcga gtttggcgtt gggcaggacg aggatgccgg tattgatttc
     6541 tttcacggct ttttggacgg cgtcggcgtc tttttcttcg acgatggcgg ttacgctgcc
     6601 gttgctgtcg cggatgatac gccccaagcc tgtcgggtcg ttgggaacgt cggtcaacag
     6661 cccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg gtttcaacgt caattaaagg
     6721 aacgtcgccg tacaacacca gcgtgcggcc ttcggcggaa aggtggggca gggcggtttt
     6781 gacggcgtgg ccggtaccga gctgttcggt ttgttcaacc caaacgacat cgcgtttgac
     6841 ggtgtccaag acttgctctt tgccgtggcc gatgacgacg cagatgtttt gcggattcag
     6901 tgcggctgcg gtgtcgataa cgcgcccgac catgggcttg ccgccgatgc ggtgcagcac
     6961 ttttggcatt ttggaataca tgcgcgtgcc tttgccggcg gcgaggatga cgatgtttaa
     7021 agtgttttgc ggcatgacgg tttcctgtgc aatgccgtct gaagcggctt cagacggcat
     7081 agggtaggtt tatcggtttt gaaactttgg tttttgccag tgttggcgat gctcttcgtc
     7141 ggcgttgttg ccggtttggt tgggtaacac ggcatggcgt tcgggacggt attggttgta
     7201 gttcatattt ttcgagtagc tgccgtcttg gtaataaacg ggcgtgccgg cgggatattt
     7261 ttgacggacg gcggtcttgc cgttgccgtc ttgataagtt tcccacgcgc agcccgacaa
     7321 aagggcggcg gcagcggtca ggaagaggaa ggttttacgc atggcttttc tttcgtattt
     7381 tcggggggta gggggtattg taatgatttt ggcggtgttc tgacaaagtt tctgcatacc
     7441 gagccagttg cgccatatcg cttacggagg catcgataaa gggcagcgcg tgggattttg
     7501 caccgaaccg gacggttttc atacccagcg cctttgcctg atgcaggttg tccgcgctgt
     7561 cgtccaccat aatgcagcat tcgggcggta cgtccaacag gcggcagaca ttgagatacg
     7621 cttgcggatt gggtttgtac agcagcccga aatcatccgt gccgaaaagc gcgtcgaaac
     7681 ggttttccaa accgagtgcg ttgacaacgg cacggacgta aaacgacggg ccgttggaaa
     7741 aaaccgcctt gcgccctttt aggcggctca gggtgttttg tgtttcaggc atgccgtgca
     7801 gcctggtcag gattgcatcg atcggatggc tttcgcgcaa aaattcggcg atgtcgattt
     7861 cgggatggtg gatttgcagt ccggcgagcg ttgcgccgta gcggtgccaa tagtcttgac
     7921 gcaggtcgga cgcggcagat tcggagagtt tgaggcggcg tgccatatag cgtgtcatag
     7981 cgcggttgat gagtgtgaag atgcctgcgt cggcatcgtg cagcgtgttg tcgaggtcga
     8041 acagccacac ggtcgggttt tcttgcatgt tgaaccgtga aaatttgtta gaatgttatt
     8101 ttacagcgaa tagaggagga ctcggaatga aacggaaaat ttggctgctg ccgctgctgg
     8161 cggtttcggc atacctgcag gcgcagacgg aagtcaggct ggcggtgcat aagtcgttca
     8221 gcctgcccaa agggttgatt gcgcgcttcg agcgggcaaa cgatgcgaag gtgtcgatta
     8281 ttcaggcggg cggcgcgaac gaaatgctca acaaactgat tttgagccgc gccaacccga
     8341 ttgccgacgc ggtgtatggt ttggacaacg ccaatatcgg caaggcgcgg gaaatgggca
     8401 ttttggcggc ggcgcaaccc gaatccgccc ccgtcgcggt cgggctgcct tcggctttgg
     8461 cggtcgatta cggctatgtg tccatcaatt acgacaaaaa atggtttgaa ggcaaaaagc
     8521 tgcccctgcc gcaaaccctg caggatttga cccgccccga atataaaaac ctattggtcg
     8581 tgccgtcccc cgccacgtcg tccccggggc tgggcttcct gatggcgaac atcagcggtc
     8641 tgggcgaaga aagcgcgttc aaatggtggg cacagatgcg gcagaacggc gtgaaggtcg
     8701 ccaaaggctg gagcgaggcg tattacaccg acttttcgca caacggcggc gcgtatccgc
     8761 tggtggtcgg ttatgccgcc agcccggcgg cggaagtgta tttttccaaa ggcaaataca
     8821 gcgagccgcc gacgggcaac ctgtttttaa aaggcggcgt attccgccag gtcgaaggcg
     8881 cggcggtctt gaagggcgcg aaacagccgg aattggcggc aaaactggtg caatggctgc
     8941 aaagtcggga agtgcagcag gcggttccgt ccgaaatgtg ggtttacccc gccgtcaaaa
     9001 acacgcgcct gcccgacgtg ttccgcttcg cccaagcccc gacgcacacc accgcccccg
     9061 cgcagcgcga tattgatgcg aaccagcgcg gatgggtttc ccgttggatt agaacggttt
     9121 tgaaataaaa caaacatacc tccccgcagg gcttcatacg gcatttttac acctgtgccg
     9181 attacgccgc acggggcgga tgttcgatca agaggaaaac aatggacttc aaacaatttg
     9241 attttttaca cctgatcagt gtttccggtt gggagcatct ggctgaaaag gcgtgggcgt
     9301 tcgggctgaa ccttgccgcc gcgctgctta tttttttggt cggaaaatgg gcggcgaaac
     9361 gcattgtcgc tgtgatgagg gcggcgatga cgcgcgcgca ggtcgatgcc acgctgatta
     9421 gttttttgtg taatgttgcc aatatcggct tattgatttt ggtgattatt gccgcattgg
     9481 gcagattggg cgtttccaca acatccgtaa ccgccttaat cggcggcgcg ggtttggcgg
     9541 tggcgttgtc cctgaaagac cagctgtcca attttgccgc cggcgcactg attatcctgt
     9601 tccgcccgtt caaagtcggc gattttatcc gcgtcggcgg ttttgaagga tatgtccgag
     9661 agattaaaat ggtgcagact tctttgcgga cgaccgacaa cgaagaagtc gtgctgccca
     9721 acagcgtggt gatgggcaac agcatcgtca accgttccac actgccgctg tgccgcgccc
     9781 aagtgatagt cggcgtcgat tacaactgcg atttgaaagt ggcgaaagag gcggtgttga
     9841 aagccgccgt cgaacacccc ttgagcgttc aaaacgaaga gcggcaggcc gccgcctaca
     9901 tcaccgcctt gggcgacaat gccatcgaaa tcacattatg ggcttgggca aacgaagcag
     9961 accgctggac gctgcaatgc gacttgaacg aacaagtggt cgaaaacctc cgcaaagtca
    10021 atatcaacat cccgttcccg caacgcgaca tacacatcat caattcttaa acgccgtctg
    10081 aaagaggagt gggaaatgga cgcgctgcac accatcgccc gaaacctgac gaaaaaacgt
    10141 caaaccgtaa gctgtgccga atcctgtacg ggcggaatgc ttgccgccgc attcacaagc
    10201 gttgcaggca gttcgcaatg gttcgaccag agttttgtaa catacagcaa caaagccaaa
    10261 gaagaccgct tgggcgtgtt gcccgaaacc ctgctcgaac acggcgcggt cagccgccaa
    10321 accgtctatg agatggcgcg cggcgcgaaa gccgtggcgc aggcggatta cgccgtcggt
    10381 atttccggca tcgccggtcc gggcggcggc agcgaaagca aacccgtcgg cacggtttgg
    10441 ttcgggtttg cctttccggg cggaagttgc gaagcaatgc gccgttttga cggcaaccgc
    10501 gaatccgtcc gcgcgcaggc ggtcgccttc gcgttggaac ggttggcggg gctgattgaa
    10561 aacggcggcg atgctgtcta aacaaaatct ccgtctgaac aaaatcccca tcggataaaa
    10621 aatgccgtct gaaacgtttc gggtttcaga cggcattttg tcggggtagg cggcggtgcg
    10681 gcttatttca ctttaccttt caacgcgcca tagcctgccg cgtccatttg ttccagtggg
    10741 atgaatttca agctcgcgcc gttgatgcag tagcgcagtc cgcctttgtc gcgcgggccg
    10801 tcggggaaga cgtgtcccaa atgcgagtcg gcggcgtggc tgcgcacttc ggtgcggcgc
    10861 atgttgtagc tgaaatcatc gtgttcggta acggattttg catcaatcgg gcgcgtgaag
    10921 ctcggccagc cgcagccgga atcatatttg tcggcggagc tgaacaaagg ttcgccgctg
    10981 acaacgtcca cataaatgcc gggtttgaac aaatggtcgt attcgtggct gaaggcatat
    11041 tcggtcgcgc tgttttgggt aacttggtat tgctcttcgg tcagggtgcg tttgagttcg
    11101 gcgtcactcg gttttttata cgttgccgcg tcgaagcctt tgccttgcgg ggcggtcttg
    11161 gttttgcccg gcagcggttc gtcagctttg cggatgtcga tgtggcagta gccgttgggg
    11221 tttttaatca agtagtcctg atggtattcc tcggcatcgt agaagttttt cagcggctcg
    11281 ttttcaacaa cgaggggcag ttggtatttt tgctgctcgc gtttgagggc ggcggcgatg
    11341 acggcttttt cggcggggtc ggtgtagtac acgccgctgc ggtattgcgt accggtgtcg
    11401 ttgccctgtt tgttgaggct ggtcggatca acgacgcgga agaaatattg caggatgtcg
    11461 tctaggctga gtttgtcggc atcgtaggtc actttgacgg tttcggcgtg gcccgtatgg
    11521 cggtaggaca cgtcttcata gctcggattt ttcgtgttgc cgttggcgta gccggatacc
    11581 gcgtcaacca cgccgtcgat gcgttggaaa taggcttcca agccccagaa gcagccgccg
    11641 gcgaggtaaa tggtgcgcgt gttcatgatt tttgaatcct ttttctgagt gtcgggtttg
    11701 tagaacgaat gtttcaagct gcccaaatcg gcattcgggt cgcggattaa cgccaacgcc
    11761 tgcgcttcgt tgatgctgcc tttgacgatg cgctgcacgt cgccgtcttt accgattaac
    11821 gcccacgagg ggtaaacgct gatattcagg ctttgggcga tcgtgccgcc gttgtcggtt
    11881 acgacgggca gcttgggata attcaaaccg gcataccatt tttggaagtc gccgtctttt
    11941 ttctcgtgca aaaagcccgg ggaggcgacg gtaatcaggt tggcggagct gaattttgca
    12001 tcttgcgccc atttttcggt ctgtcccaat tcggacagac acaaaggaca ccagctcgcc
    12061 caaaatttaa tcagcgtcgg tttgtctttt ttcaagtaaa cactggcggg gcggttgtcc
    12121 gcagttttca aagtggataa agtgtgcggc acggtcgcgg ctccggcatc gacgattttg
    12181 ggcgaacaag cacccagcgc aagcaggcag ccgaacttgg cgcaaaggga aaagaaagta
    12241 cggtgtttca ttttgatgtt tcctgtgtgg acggtttgca tgattagacg tttgagatgc
    12301 cgaaacctta cagcccggat tttcagacaa ccttaccgcg taaaatacgc tacaatacgc
    12361 cctgtttcaa gtttctaaaa ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag
    12421 aaaaaacagg aaacgccggc tctcgaggag gctcaaattc aggaaaccgc agcaaaagca
    12481 gaatctgaac ttgctcaaat agttgaaaat attaaagaag atgctgaatc tttagcagaa
    12541 agcgtcaaag ggcaggtcga atctgccgtt gaaaccgtca gcggtgcggt tgaacaggta
    12601 aaggaaaccg ttgccgagat gctgtctgaa gcagaggaag cggcggaaaa agcagcggaa
    12661 caagtcgaag cggcaaaaga agccgttgcc gaaaccgtcg gcgaggctgt cgggcaagtt
    12721 caagaagccg ttgcgacaac tgaagaacac aagctcggtt gggcggcgcg tttgaaacaa
    12781 ggcctgacca aatcgcgcga caaaatggcg aaatcgctgg cgggcgtgtt cggcggcgga
    12841 caaatcgacg aagatttata cgaagagctg gaaaccgtgc tgattaccag cgatatgggc
    12901 atggaagcca ccgaatacct gatgaaagac gtgcgcgacc gcgtcagcct caaagggctg
    12961 aaagacggca acgaattgcg cggcgcgttg aaagaagcct tgtacgacct gattaagcct
    13021 ctggagaaac ctttggtttt gcccgaaacc aaagagccgt ttgtcatcat gcttgccggc
    13081 atcaacggcg cgggcaaaac cacgtctatc ggtaaactcg ccaaatattt ccaagcgcag
    13141 ggcaaatccg tattgctggc ggcaggcgat actttccgtg ccgccgcgcg tgagcagctt
    13201 caagcttggg gcgagcgcaa caacgtaacc gtgatttcgc aaaccacggg cgattccgcc
    13261 gccgtgtgct tcgatgccgt ccaagccgcc aaagcgcgcg gcatcgacat tgtgctggcc
    13321 gacaccgccg gccgcctgcc cacgcagctt catttgatgg aagaaatcaa aaaagtgaaa
    13381 cgcgtgctgc aaaaagccat gcccgacgcg ccgcacgaaa tcatcgtcgt gcttgatgcc
    13441 aatatcgggc aaaacgccgt caaccaagtc aaagcctttg acgacgcatt ggggctgacc
    13501 ggtttaatcg ttaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgcc
    13561 tccgaccgcc ccgttcccgt ccgctatatc ggcgtgggcg aaggcataga cgacctgcgc
    13621 ccgtttgacg cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa
    13681 aacagcagac cgatgccgtc attcccgcgc aggcgggaat ccagaccttg ggataacggc
    13741 aatattcaaa ggttatctga aagtccgaga ttctggattc ccactttcgt gggaatgacg
    13801 ggatgtaggt tcgtgggaat gacgtggtgc aggtttccgt atggatggat
//
LOCUS       DAWISC010000038        13385 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWISC010000038 DAWISC010000000
VERSION     DAWISC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.038"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(110..538)
                     /locus_tag="WIW87_000790"
     CDS             complement(110..538)
                     /locus_tag="WIW87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ5173981.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WIW87_000791"
     CDS             complement(603..2351)
                     /locus_tag="WIW87_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ5173982.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WIW87_000792"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WIW87_000792"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ5173983.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WIW87_000793"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WIW87_000793"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ5173984.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WIW87_000794"
     CDS             4754..4924
                     /locus_tag="WIW87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ5173985.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WIW87_000795"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WIW87_000795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ5173986.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WIW87_000796"
     CDS             6085..6279
                     /locus_tag="WIW87_000796"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ5173987.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WIW87_000797"
     CDS             6481..7890
                     /locus_tag="WIW87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5173988.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7991..11200
                     /gene="recC"
                     /locus_tag="WIW87_000798"
     CDS             7991..11200
                     /gene="recC"
                     /locus_tag="WIW87_000798"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ5173989.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11268..12836)
                     /gene="mtrF"
                     /locus_tag="WIW87_000799"
     CDS             complement(11268..12836)
                     /gene="mtrF"
                     /locus_tag="WIW87_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ5173990.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYIVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13168..13380
                     /locus_tag="WIW87_000800"
     CDS             13168..13380
                     /locus_tag="WIW87_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5173991.1"
                     /translation="MKNEIQKNMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3355 a         3230 c         3543 g         3257 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggtgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 tacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcaacaccat gccgcgcaat cagggttggt atcacacgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggc gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg
     7081 tttggaactt gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg
     7141 ggtatttggg gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt
     7201 gtcgaaccga accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg
     7501 gtacatatcg accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg
     7621 agcgtgttgg cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgaccgcac tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca
     7921 ttttgtttga aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt
     7981 tgtcgaaatc atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt
     8041 tgcccgcatt caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt
     8101 gcagagtcag gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc
     8161 ggcgaatttg tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat
     8221 tcccggtatt ccggaactca gcccgtttgc acccgaagtc atgcgctggc ggctgctgga
     8281 tttgttccgc agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct
     8341 gcaagactat ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga
     8401 catattcgac caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag
     8461 gcggctcggt ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga
     8521 tggcaggcag agcgcgccgc accgtgtcgc gttgtgggaa aagctgcttg ccgcgttgga
     8581 caaggataag ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta
     8641 tcttcagctt ttgcacaagc tgtccgaaca ttgcgatgtg ttcgtgttcg cactcaatcc
     8701 gagcgggatg tattggggca acgtcatcga agcggcgcaa atcctcaaag gcggcggcga
     8761 tcccgattta actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcgcga
     8821 ctttttcgac tttttgaacg aaatggaaat agaaggagaa acgccggtat ttgaagaagg
     8881 cgggcgcgat acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc
     8941 tgaaaatgtg ggaagcgtca acacgggcga cggctcgata cgcatcgtct ccgcacacag
     9001 ccccttgcgc gaattgcaga tactcaaaga caagctgttg aaaattctgc atgaacatcc
     9061 cgattggcag ccgcatgata tcgccgtatt aaccccgcac atcgaatcct atacgccttt
     9121 tatcgaagcc gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc
     9181 agacgtgaaa atcagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt
     9241 gttggaaagc cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt
     9301 gcgccgtttc ggactgactg aggacgattt gccgcttttg cacgatatgg ttgccgattt
     9361 gaacgttcac tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg
     9421 gaagcaggcg gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaacccgat
     9481 gtggcaggat gtcagcgcgt ggtatgccga cgtgaaccaa accgccctgt tcggacgttt
     9541 tgccgccctc ctcgaaaccc tgtcggatat tgtacggata tggcggcagc ccgcaacgat
     9601 cggcgaatgg gtggcgcgtt gccgggattt gcttgaaaca ttgttccaag ctgaagccga
     9661 tgaccaaaag tcagtccaaa accttgaaaa cgaatgggtc aaatggcagg cggaaacaga
     9721 attggcgcaa ttttccggac agttgccgca acaaaccgtc atccgccata tccgacgttt
     9781 cctcgacagc gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt
     9841 gccgatgcgg agcctgccgt tcaaagtcat ctgcctgttg ggtttgaacg acggagattt
     9901 tccccgtaat accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggaga
     9961 ccgcgcccgc cgcgatgacg accgctacct gttcctcgaa gccctcatca gcgcgcgtga
    10021 aatcctctac ctgtcctaca tcgggcgcga catccgcaaa gacgaagagc ttgccccgtc
    10081 ttccctgttg ggcgaactca tcgataccgt tgccgctatg gcgggcatcg gtagccgcca
    10141 acttgcacaa aacctgatag aacagcatcc gctgcaagcc ttctcgcgcc gatattttca
    10201 agaaggcgga cgttcagacg gcatattcgg cacgcgtacc gactacgccg ccgcgctcgg
    10261 acaaacgccc gaaccgccgc aacccttttt cgatcaaccc gtagaaaacg ccgaacctgt
    10321 tgccgaaatc ggacaggacg aatttatccg tttctggcgc aaccctgtca aagtatggct
    10381 tcagcagcag cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc
    10441 cttcgagccg caacacgccg atcaaatcgc cgaaatctac atcgaagcac ggcgcgaagg
    10501 acgggatttt gcccaaaccg ccgcccgcat cggggcggaa agcctcctgc cgtcgggaga
    10561 gttgggcaga ctttggcagc aggacttcca aactgctgcc aaacaaatcg acacggcggt
    10621 tttaaacagc cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct
    10681 gaaaggcagc ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc
    10741 caacgcgccg caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc
    10801 gtctgaagcc gaaatgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc
    10861 ggaaatcgcg caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat
    10921 cgggcaaaac cgcccgctgc cgttttttgc caaaacctcg cttgccaccg ccgaagcgtt
    10981 tgcccaaaaa caagattggg aagccgccct gaaaaaagcc caaaccgcct accacggcag
    11041 caaagtcagc aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag
    11101 ccaaaacccg ctcgaacagc ccctgtttga aaacctcgtc cgtctgcttg ccgacacgct
    11161 tgccgcagcg gaaaaaaagg aagaggccgg agaagcctga cgggatagaa aggcaaatgc
    11221 cgtctgaaag cgtttaagca tttcagacgg cattttgttt atcgtgttta aggtgcggga
    11281 tagaatgtgg gcgcgccggg accgacgggc aggcccaaaa caaataccca aatgcagaat
    11341 aaggcaatcc acgcaatcaa gaagaaagcg gaatacggca acatcataga aatcagcgta
    11401 cccacgcccg catctttttt gtatttgatc accgtcgcca taatcagccc gaaataactc
    11461 atcatcggcg taataatatt ggtaacggaa tcaccgatgc ggtaagcggc ttgaatgact
    11521 tcgggcgcgt agccggccaa catcagcata gggacgaaaa tcggcgcagt taccgcccat
    11581 tgcgcggagg cggagcctat catcagattg ataaaagcac aaattaaaat aaaaccgata
    11641 aacaacacgc tgccgcccaa gccgacttct tttaagaacg tcgccccttt aacggcaata
    11701 tattgcccaa tattcgtcca attaaaaaat gcgacaaact gtgcggcaaa aaagatgatg
    11761 accaaataaa gccccagagt actcatcgat tcggccatcg cattaacgac ttcctgttcg
    11821 ccgcgcaaac ttcgggttac ccggccataa acaatgcccg gcagtgcaaa caacaagaaa
    11881 ataaaaacaa caatcgattt taaaaacggc gaaccggaaa ccaatcctgt ttcaggatga
    11941 cgcaaaatac cgtcggcagg gacgatgctc caagccaata gggcggataa ggcaacaaac
    12001 accacgccag cccaaattaa tcctttatat tccaaaggcg tgatttcatt ggaatgccga
    12061 atgtcttttt cttcttgtga caaatctgat tgataagggc ccaattgcgg ttcgacgatt
    12121 ttttcagtaa caaaataacc aatcaaagca atcacaaacg tactggctac cataaaaaac
    12181 cagttggctt cagggcctac gacgtagtcg ggatggatga tttgcgccgc ctgttgggtg
    12241 atgcctgcca agagcggatc gattgtgcct aagaacagat tggccgaata accgcccgaa
    12301 acgccggcga aagccgcagc cagaccggca agcggatggc ggccgaggga atgaaagatg
    12361 atggcggaca aagggattag gacgacatag cccaattcag aagcggtatt agataaaatc
    12421 cctgtaaaaa caaccataaa agtagtgagt ttgcgtggcg attttgtgag caataagcgc
    12481 attaatgcgg aaatcaagcc cgatttttcc gcaatcccca cgcccaataa agaaaccaac
    12541 accgttccca acggcgcgaa accggtgaaa tttttaacgg tatgcgtcag gattttgatc
    12601 aaaccgtcgg cattgagcag gctgacaatg taaatcaaac cgtcatcggc acgtcctttc
    12661 gcaccaacag ggcgcggatc ggggacggat agtccgaaat acgcaccgac ggcagaggca
    12721 atcagcaata acacaatgaa aataataaaa agcgtaaccg gatgcggcaa catattgccc
    12781 agccattcga ctgtgcgtaa aaatcgtccg tcccgttgcg tatcggtttg actcatctgc
    12841 ttctccttaa aaaaatatat tgagtgcatt aaatttttga atgtaacatg aagtgttttg
    12901 taagcaaact aaaataatcg catatgtata taaccgcaat agattaaaac agaaaatgcc
    12961 cacatcgtta ttctcataaa ggcggtaatc cgaaaacttg acgttaaagc cttatcagga
    13021 atgactggaa ctcaaaaaac ggtttcccgt tttcgcggaa atggcgggat tttcagtttt
    13081 tatttgcaaa aatgctggag aaaaatgcag tttacatttt gaatggtatt gttattaatt
    13141 gtaaataaat attggaaggt aaatgatatg aaaaatgaaa ttcaaaaaaa tatggacaaa
    13201 tataatccct ggcatgaaga tgattttgag tcgtatgaag acattgccag agatgtatcg
    13261 ctgacgacag ataaaacgtt cattgaacat tatttgttag aagtttattc agaagaaaac
    13321 ggacattttg accaagagaa tgtccatgca atgatagaag aaattaaaaa tgcaatttag
    13381 tgaat
//
LOCUS       DAWISC010000039        13171 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWISC010000039 DAWISC010000000
VERSION     DAWISC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.039"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            201..413
                     /locus_tag="WIW87_000801"
     CDS             201..413
                     /locus_tag="WIW87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ5173992.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            561..2144
                     /locus_tag="WIW87_000802"
     CDS             561..2144
                     /locus_tag="WIW87_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ5173993.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2226..3323
                     /locus_tag="WIW87_000803"
     CDS             2226..3323
                     /locus_tag="WIW87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ5173994.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3437..4552
                     /locus_tag="WIW87_000804"
     CDS             3437..4552
                     /locus_tag="WIW87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ5173995.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4677..5132
                     /locus_tag="WIW87_000805"
     CDS             4677..5132
                     /locus_tag="WIW87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ5173996.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5203..5679
                     /locus_tag="WIW87_000806"
     CDS             5203..5679
                     /locus_tag="WIW87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ5173997.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5724..6017
                     /locus_tag="WIW87_000807"
     CDS             5724..6017
                     /locus_tag="WIW87_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ5173998.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6014..6286
                     /locus_tag="WIW87_000808"
     CDS             6014..6286
                     /locus_tag="WIW87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ5173999.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6469..7386
                     /locus_tag="WIW87_000809"
     CDS             6469..7386
                     /locus_tag="WIW87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ5174000.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7451..8008
                     /locus_tag="WIW87_000810"
     CDS             7451..8008
                     /locus_tag="WIW87_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5174001.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            8381..10177
                     /locus_tag="WIW87_000811"
                     /pseudo
     CDS             8381..10177
                     /locus_tag="WIW87_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            10190..13129
                     /locus_tag="WIW87_000812"
     CDS             10190..13129
                     /locus_tag="WIW87_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ5174002.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFLDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFADLFTAYLHENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGR"
BASE COUNT         3693 a         3146 c         3304 g         3028 t
ORIGIN      
        1 tgcgccgatt gccaaggcaa tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 tgtcctgatg ggaattgccg ccattatgcg caagcgtccg tattttttca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagaatgcag
      181 taccaacgaa aggaaaaatc atggaaaccc ttatcctcga catcggcggc atgagctgcg
      241 gcggctgcgt caaaagcgtt acccggatat tggaaggcgt aaaaggcgtg gcaagcgtcg
      301 aagtcagcct tgaaaacaaa agtgcgaccg tcggatacga ccccgcgcaa accgatgcag
      361 gggcgttgat tgaagccgtt gaagacggcg gctatgatgc cgcgttgaaa taaagcggca
      421 aaaatgccgt ctgaagcctt cgcgcctccc agatgccgtc tgaagtacgc tccgcgcctt
      481 cagatggcat ttgatttagt tgaacgggta ggatgaattg gttaaacttg cgggcaatag
      541 tgccttcaga caaacaaaat atgccgaaca agaagaaaaa agccgccttt gcgcgtgctg
      601 caacggagaa cggtgtgccg gacgtgtcca acatcaaaaa ttcatatgat gatttgatgt
      661 acgaatccgg cgcattctct caaaccgcca tcaataattt ggaggcgcgc gcccgcctga
      721 tgggtttgca gcccgcgccc gccgccaatg ccaaggtctt ggagctgggc tgttcgatgg
      781 gcggaaacat catcacgcag gcactttatt acccggatgc ggaatttgtc ggtatcgacc
      841 tgtccggcag gcaggttgca cagggcaacg ccatcattga aaaaatgggc ttgaaaaatg
      901 tgcgtctgga agaaaaagat attttgacca tcgatgagtc attcgggaag tttgactata
      961 tcatcgtcca cggcatttgg tcgtgggtgc ctgacgcagt taaagacaaa attttttcga
     1021 tttgctggaa caacctgacc aaacacggca ttgcctatat ttcatacaat gtttaccccg
     1081 gctggaaacg gcaggagcag ttgcgcgaaa ttatgtactt tgccggcagg gatgtgcttg
     1141 aagaaccctt ggaagcgcgg acgcggaaag gcttggacgc gctcaaggcg ctggcggaaa
     1201 ttttggaaaa cgacaagggc ttggacggcg gcggcaaact tccggcgatt caaaaaatat
     1261 tgaatcataa tttttattac atcgcacacg aatatatgga ggcgtttaac gacccgattt
     1321 acgtcaacgg cttcatcgaa tgggctaacc gccacaggct cgcatatatc ggggatacca
     1381 atttgcacgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attctggcat
     1441 tggcagggga cgattatatt gccaaagaat tttacagtga tattttatcc gaccgccaat
     1501 tccgccgttc gcttttatgc cgtgaggaag tcggggatac tgtcaggcgt gatgagtcgg
     1561 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acaatcaact
     1621 ttgatgaaaa cgatatcctg ctttcgggca tacgcgatgt gatgaaaacc ggagaggcgt
     1681 ttaaaacgga agatgttgcg gaaaatctcg cccgccgatt tcccggtttg gaatttgacc
     1741 gcatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
     1801 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgcttta
     1861 caaactatgc cgccgccttt gtggaacacg gcgcggaagc gtttgtccgg cctgccaacc
     1921 gttacaacga aagcaccccc tcattcggat acgggcattt gtacattatg cgccaattgt
     1981 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
     2041 gcaccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
     2101 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccggcg
     2161 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaag cggtgcacga
     2221 cacatatgaa ccagacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
     2281 aaaaccacct gctgctcaat gcctaccagt tggcgcataa ttcttcccag gcttcgcgca
     2341 aactctcgtc cggccaactt caaaccgaaa tcaggggaat gcttgagcaa aaccactata
     2401 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
     2461 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
     2521 cggtcgtgct ggtgtccggc tgcaaaaaag aacgtgcaat cgagatgaag ctgccgacgg
     2581 aggcattgtt tgcctgcctg caaaactatc cgcacctgcg cgcgttgacg caagagacgc
     2641 aatggctgcc ttatcttgtg cattcttccg atttgagcgc ggtcgcgccg gatgagtggt
     2701 ggcgtgccaa acaaaatacc gaagcggcgg cgcaacactt gcgccgtttc gccccgcgcc
     2761 ctttgctgtt gcccgaaggg cagtccgtcc acgtcgttta cgcgctgggt ttcggcagcg
     2821 gcaaagttca gacggcattg ggtcagaacc ttcttcaggc aggtctgccc ctgatgcagg
     2881 tatggcagga aaatcttgca tcggaaggcg ttacgctgtt tgccaatccg ctttccccgg
     2941 attctccggt acgcgcgctt tcagacggca gccacacgcg ccaacgtatg gcgatggatg
     3001 tgtttgcggc aaacgcgata cgcgccgtcc gtatgcagag tccgcgcgtc ggcgtggtcg
     3061 ctgcggcaaa ggcgggcgga cagattttat tcgggtttaa tgcgaccgac ggcgcgtttg
     3121 aagtcgtgcc gcaggtgttt tcgtggcagc tttcctttac cgacaacatc gccgtcatcc
     3181 agcaaaattt cctcgacctg atggcggagt gccgcgtgga acacgtttac ctgttgcaca
     3241 atcccttggg cgaacaggaa agcatcccga gctatgcgga agcattgaaa cgggaagggc
     3301 acaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttatataa
     3361 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
     3421 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
     3481 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
     3541 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
     3601 tcttgaccta gaagaaaaat gtctccaagt gacaggacct tttggacaaa aagaagtcat
     3661 gcgctatttt caaaaagaac aactagccct attcgaagtt gctgatttgg ttggtcttgg
     3721 aaaaatcccg ctagagaaac gaaatccatt acaaatccaa actcgtggta ttggagaatt
     3781 gattcgccac ctcattagtc aagaaattaa agaaatctat attggcgttg gcggtacggc
     3841 cagtaatgat ggaggtattg ggattgctgc tggtttaggt tatcaatttt atgatgagga
     3901 tggaaatgcc ttacccgttt gcggtcaatc cttactaaac ctagcttctg tttcaacaga
     3961 aaatcgctat gaaattcctg aagatgttca cattcgtatt ttagcagatg ttgtgagtcc
     4021 cttatgtgga caccaaggtg cgacctatac gtttggcaag caaaaagggt tggattctac
     4081 tatgtttgag gccgtagatc aggcaataca agatttttat gaaaaagtct cccctgcaac
     4141 attaaaactt aaaggagcag gagctggtgg aggcatcgct ggtggtttgt gcgcctttgc
     4201 tcaggcaagt atcgtatctg gaattgacac ctgcctggac ttgattgact ttgataagaa
     4261 agtgtcagat gttgacttgg ttatcgttgg tgaaggaaga ctagatcgtc aaagtttagc
     4321 agggaaagcg cctattggtg tagcaaaaag aacccctgtc ggagttcctg ttgtcgctat
     4381 ttgtggcagt ctcgttgaag atttgccttc cctgccattt gaaaatatcc aagctgcctt
     4441 ttctattttg gagaaaagtg aacctttaga agatagtttg aaaaatgcca gcctctattt
     4501 ggagcatacg gcttctaata ttggacactt attaaatatg cctaagattt agccaaacca
     4561 tttcttccag atggatgttt tggctggttc tgccttatgc ggttacgatt aaccgaccga
     4621 tgccgtctga aagggtttcc gcttcagacg gcatcgcgcc ggaacaggga ggggatatga
     4681 aaaaaccgaa aatccttttt gtctgcctcg gcaacatctg ccgttcgccg atggcggaat
     4741 acattttgcg ccgccgcgcc gccgaagcgg gcattcccct tgaagcggac agcgcgggga
     4801 catcgggctg gcacgacggc gaagatatgc accgcgagac ggcaaagata ttgaaaaaac
     4861 acggtatcga tgcttcaggc tttaccagcc gcaaaatccg ccaaagcgat gcggcggcgt
     4921 ttgactgcat catcgcgatg gacggcaaga atttgtccga attggaaaga accttcggca
     4981 ggcggccgga aaaaatattc aagctgaccg acctgatacc cgaaagcggt tacgaccatg
     5041 tccccgatcc gtggtatacg ggcgattttg aagaaacctt caggcttgcg gatgcgggct
     5101 gtcgggcatt gttggaaaag atttccaaat aaagcaattg aatacaaata taaaaccgcc
     5161 tcctgccgta tcggttgttc agacggcata aacagagaat ttatgaaaac aaatttcaaa
     5221 cagaaaatta tcgaacaggc acgcagcgaa ggcttgcagg taaccgcttt gcgcgagcag
     5281 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaacgt cttgtcgcag
     5341 atgcagcagc aaagcgaggg cgtgcttgca ccgcctaccg cctaccgcgc ccttgatttt
     5401 tgggcggagc agggcgtttt gcacaaagtg gcggcggtca acggctatat tttgtgcagc
     5461 cacgcgcagc acgagtgcga cgaccattgc cacgaccacg aagaagccga agcgcaccac
     5521 agcgcgttta ttttggtctg caccgaatgt ggcacggcgg acgagcaaac cctgagccac
     5581 gagtgggcgg cactgcgcgc aggcgttgcc gaaagcggct ttgcgctgaa agaagaacac
     5641 gttgttttaa ccgggatttg taaaaaatgt cagcagtaat cggtggtttg cattgataat
     5701 aagcctttcg aaaggagcag ataatgaaac cgtctctttt gttgcaaagc agaaagaaag
     5761 aaatattggc agttttcggg aaatatccat tgatttgcaa tccaagggta ttcggttcgg
     5821 tttctcgcgg agatgatacg gaaaacagcg acattgattt gttggtggat gcaaaaacag
     5881 ggacaacatt attggatttg ggcgggctgc aagaggaatt gcagaaactc ttgggcataa
     5941 aggtcgattt gctgacaccc gatgacatct cggcccactt tagagataag gtgttgactg
     6001 aggctgtcgc attatgaaaa tgcagaaaga attgtcggtc tatttgaaac agatattgca
     6061 ggcagcgcaa tatatccggc tatataccga caaaatggat tatgggcaat tttctgccga
     6121 cacaaagact gtgcaggcag tcgtttttaa tttgttcctt atcggtgaaa acgcaaccca
     6181 tatccttaaa tcgtatccgg aatttgccga ggaaaccaaa tatttgaatt ggatagggat
     6241 gagggggtat tgcgcaatag gattgcccat ggctactttg aaatgaacct gtctgtcgtt
     6301 tgggagacgg ttttgaacgt cataccggcg atgtcagccg atatatcgaa tttgctggaa
     6361 caattgtcaa ttgatgaaga acatgataac agctgccatc acttttaaca ttatttaatc
     6421 atttaaaaca ccctatcttc aaattttaaa gtgggacatc acatagaaat gaaaaaaacc
     6481 aaagtccacc tgatttcagg ttttctggga acaggcaaaa ccaccgcgct caaaagcctg
     6541 atggcgcaaa aagacccgaa cgaaaaatgg gtcatcatcg tcaacgagtt cggcgaaatc
     6601 ggcattgacg gcgcggtatt gagcgacaac ggcatccctg tggcagaaat cgccggcggc
     6661 tgtttgtgtt gcaccgccgg cccgcaaatg ggcgtaaccg tgcagaaaat gctgcgcgac
     6721 gccaagcccg accgcctgat gattgaagca agcggactgg cgcacgccgc cagtgtcatt
     6781 gatgaactga aaaccaaacc gctggacagc cttttggaaa tcggcgcagt ctttaccgtc
     6841 gtcgatccgc gccagttcat caaccccgat tacgcgcagc aggcattgta taaagaccaa
     6901 atcggcatct gcgacgtatt ggttgccagc aaaaccgatt tatgcacccc cgaacagctt
     6961 gccgaatttc acgacaaagc cgcaaaactg ttcccgccca aggctaaagt ggtcgaagtt
     7021 caaaacgcac aactcgatat ccaatggctt gacatccccg tcatcgaaaa atcacgctac
     7081 cgcctcaaag ccctgccgga caacacgatg ggcttccagt cgcaaggttt cacattcccc
     7141 gccggacgcg atttcgacgg tgaaaaattg accaacttct tcaatgattt gcccaatatg
     7201 accgaaggac tcgtccgtgc caaaggcgtg ttccaagtat tgggaacgtg ggtgtggctc
     7261 aactgggtgg acgggcaatg gggtgcgaac caagtgtctt ggcggcgcga ctcgcgtttt
     7321 gaattgattg ccaaatcgtt tgatgcggat ttaatcgaac aaaaactcaa agacgcattg
     7381 gaatagcgtt aacgagcctt cagacggcat aggaaatgaa aatgctgtct gaaggtctga
     7441 ggaggacggg atgaaaccca aattcaaaac cgttttaacc gcgctgcttt tggcggtttc
     7501 cctgccgtct atggcggcaa cccatgtttt gatggaaacc gatatgggca atatccgttt
     7561 ggttttggac gaatccaaag cccccaaaac cgttgctaat ttcgtgcgct atgcccgaaa
     7621 aggcttttac gacgacaccg tttttcaccg cgttatcgac ggttttgtta tccagggcgg
     7681 tggattgacc gaggacttgg cacaaaaggc aagcgataag gccgttgcca acgaatccgg
     7741 caacggcttg aaaaacaccg ccggcaccat cgccatggcg cggacgacag cccccgattc
     7801 cgccaccagc caattcttta tcaatctggc ggacaacgct tcgctcgact acaaaaacgg
     7861 acaatacggc tataccgttt tcggcagggt cgaaagcggc atgaacaccg tttccaaaat
     7921 cgcccgcgtc aaaaccgcca cgcgcggctt ttatcaaaac gtacccgtac agcccgtcaa
     7981 aatccgtcgc gttgttgtcg ggcagtaaca cgcagacaga cgttcagacg gcgtcgcccg
     8041 tttcccaaaa aacgccgttt aaagtaaaaa aatattttaa aacagacagt tgatattgac
     8101 aaattcaaac cgaggatttt aaaatgctgc caacccaacc caacccaacc caacccaacc
     8161 caacccaacc caacccaacc caagccctga tctaagcgac acagccgggg cgaacactga
     8221 ggcaatctac acttcagacg gcattaccgc caactctacc caactcgaac agctcaaaaa
     8281 actgtttccc gcctgttttg acgcagacgg aaatttcctt atcgacagat tacaagccga
     8341 aatcgcgccg cagaccgaca tcggacgcga attttacgaa atgaactggc tgggcaaatc
     8401 atatgctcgc ctgcttcgca acctgccgcc cgaaaccctg ataagtgaag acaagacgca
     8461 caacgccaag cccgaaaatg caggcagcca aaacctgctg attcgtggcg acaatctaga
     8521 agtgttgaaa cacttaaaaa acgcctacac aaacagcgtg aagatgattt acatcgaccc
     8581 gccctaaaac accggatcag acggctttgt ctatcaggac gaccgcaaat tcacacccgc
     8641 tgaacttgcc cgcctagcca atattgatga agacgaagcc gcgcggattt tagatttcac
     8701 cgacaaaggc tcaaactcgc acagcgcatg gctcaccttt atgtatccgc gcctgtatgt
     8761 cgcccgcgaa ctgttaaagg acgacggtgt gatttttatc tcgattgacg ataacgaagc
     8821 ggcgcagttg aaattgttgt gtgatgaagt gtttggggaa gggaattttg ttgcacaatt
     8881 gccttggcga aaaagaacag ctaaatcaga tgtgcctttt ggtatttcgc aggattatga
     8941 atggatattt gtattcgcaa aatcttgcca atttattgca gcaactaaag gcaaggaacg
     9001 acgctattat gagactgatg atttccccga tcgtccttgg cgtacccacg acttaacgaa
     9061 acaaacaact gcggcggaaa ggccaaatag ttttttcaca atggttgatc ccaagacagg
     9121 aaaaaaatat ccagcaaatc caaatgcaac ttggcgtgta accaaagata catttcaaga
     9181 ttattataat aaaggaaaaa tcgttttccc tgatgattat gatttcttaa atatcagcaa
     9241 tccagttatg cgttacttta aagatgacga catgaaaaaa gctggcgagg attttggcaa
     9301 agtagctgta agcagtagat tacctgaaaa tgttggtaca ttagccgatg cagtagccga
     9361 atatttggct atttttagta ggacgctacc tgaaaatatc ggaatgacta aagaaggcac
     9421 aaaagaaatc acagatttat ttggtagcaa aatatttacc ttccccaagc ctagtcaatt
     9481 gattaaattt ttagtttcaa taagttcaaa gagtaatgac ctaatcctag acttcttcgc
     9541 aggcagcggc acaaccgccc acgccgtgat gcagcttaac gccgaaggac aaaacggtaa
     9601 ccgccgctat atctgtgtac agcttcccga aaaaaccgct gaaaaatccg aagcccgtaa
     9661 agcaggctac ccgaccatct tcgacatcac caaagcccgc atagaaaaag ccgccgccaa
     9721 aatccgcgtc gaacatcccg attacacggg cgattcgggc ttcaaaatct ttcaaacggc
     9781 agacaatttc aggcagcatc cggacaagga tttttcgccc gaacaaccgg atttgccgct
     9841 taacgatgaa ttaagcgaag aacagctgca aacgcttctg accacctgga cgctgtatga
     9901 cggggcggca ctgaccacgc cggttgagcc tgtgcggtta ggggcttaca cggcgtatct
     9961 gtgcgaaaaa cggctgtatc tgatgaatgc cggttttact tccgccgatt tgttggcgtt
    10021 tatccgcaag ctggacgacg atgcggattt caatcccaac cgcgtgattg tattcggcag
    10081 caatatggca agcgccatgc agcacgaact tgaccaggcg gttcgcggtt atgccaataa
    10141 aaaagagatt gagttgaatg tggttatcag ggtttgacgg aggcaattca tgagcggttt
    10201 taattacgag aaaaaccagc cgcaccaaat gcgggcggtt tcggcggttt tgggcgtgtt
    10261 tgacggggca acgcccaaat atcggacggc agacgaaaat cccgaacttt tgtttgctgc
    10321 aaaacaatac gcaaacaata tcctgaaagt gcaaagccaa aacggtatag acggccgatt
    10381 ccccgaccgt tcggacgacc aaaatatcct tgatatttcc atggaaacgg gcacgggcaa
    10441 aacctatacc tacacacaaa ccatgttcga gctgcaccgt tggctgggcg tgttcaaatt
    10501 tatcgtggtc gtgccgactt tgtccattaa ggcgggaaca cagcagtttt tgcaaagcaa
    10561 ggctttggca gagcattttg aacaggattt cggcggcgat tatgaaggcg tacgcctgaa
    10621 aacctatgtg gtggaaagcg cgaaaaagaa taagggcaaa aagtccaatg cgcccataac
    10681 gattgagcaa tttgtcaaag cggaaaacaa aaaggaaatt catgtgctgc tgattaacgc
    10741 gggcatggtt aattcgtcgt ccatgaacga tacgggcgac aaggcattga aggatttgtt
    10801 tgacaatccc gttgatgcat tggctgccgt gcgcccgttt atgattgtgg acgaaccgca
    10861 taaattcccg acccgagata gcgcgaaaac gtggggcaat atcaaacgct taaaaccgca
    10921 atatattttg cgctacggtg caacatttaa cgatgaatat tacaacttgc tttaccgttt
    10981 gacggcagta gacgcgttta acgacgggct ggtcaaaggc gtgcgcgtgt ttcaggaaga
    11041 aatgcagggc ggcatggatg cggcggtaaa actggtgtcg tcggacggca aagaagcgaa
    11101 atttgaatta aacgaaaagg acaaaaagca gacgttcaaa ctggcaaaag gcgaagattt
    11161 ggcgcaaatc catccggcta tttcggattt gaaaatcgac aaaatgaata aaaccgtggt
    11221 ggtgttaagc aacggcttgg agttgaaaac gggtgccgtc atcaaccctt attcctattc
    11281 gcaaacggtg caggatgcga tgatgcagcg ggcggttgcc gaacatttca agctggaacg
    11341 cgcgcttttg gcagaacgcg cgccacagcc caaaatcaag ccgctgacgc tgttttttat
    11401 tgacgatatc gcgggctacc gcagcggcaa cgagctttca ggcagcctga aagataaatt
    11461 tgaaagctgg attcgcgcgg aagcggcacg ccgtctgaaa acggaaagcg acccgtttta
    11521 ccgcgattac ctgcaaaaga cgttggacga tgtatccgcc tgccacggcg gctatttttc
    11581 caaagacaat acagacagcg acgatagaat cgagcaggaa atcaatgaaa tcctgcacga
    11641 taaggaaaaa ctgctgtctt tggacaaccc gcgccgcttt attttttcca aatggacgct
    11701 gcgcgaaggc tgggacaatc ccaacgtttt ccagatttgc aaactgcgtt ccagcggcag
    11761 cacgacttcc aagctgcaag aagtcggacg cggcctgcgc ctgccggtaa acgagctgat
    11821 ggcgcgggtg cgcgatgtac cgtacaaact gaattatttt gtcgatagca gcgaaaaaga
    11881 ctttgtgaag cagcttgtcg gcgaaatcaa cgacaattct tttcaggaag aaatctccaa
    11941 aaagtttacc gaagagctga aacaaaaaat attgcaaaaa taccccgata tcaaaccgct
    12001 ggtattggta aaccaactgt ttttagatgg catcattgac gacaatgaaa actttgccga
    12061 agacggctat gacaaattaa aagccgccta tcccgaagcc ttccccaaag gtttggacaa
    12121 aggcaaagtc agcaacgcca aagacgaagg caaagacacc atcatcatgc gcgaaggcaa
    12181 atatgaagaa ctcaaagcct tgtgggagct gattcaccat aaagccgttt tgcagtataa
    12241 aatcaaggat gaagccgaat ttgccgattt atttaccgcc tatttgcatg aaaacgctgc
    12301 caaattccca caggcaggca tacgcacggc ggtaaacgaa gcttatatca acaacgggct
    12361 tatgctttcc cgccgcatag acagttttga agatgaagat tttatccgtt tcaacacaat
    12421 gacttaccga gagtttctgg aaaaactggc acaaacggca aaaatccgta tgcagacttt
    12481 gcatcaggcg ttttactgca tccgcaacga actgaacatt ggcgattttt tgaatatgca
    12541 aaccatcgcc caaatcaaaa acggcttcaa ccggtttttg cttcatcatt ccttccataa
    12601 attcgaactg gattaccggc ttgtcggcag caaaatccat ccgaccaaat ttaccaataa
    12661 agacggcaaa ccgcgcgcgg tgaaaaaagc agatttgggc agatttgaag atacggagca
    12721 ccggcctgcc gccggctatc tcttcggcga gattttctac gattcggata tagaacatga
    12781 aaatgtcgcc aacaaccaaa ttgaaggcgt aatcgtattt accaaaatac cgagaaactc
    12841 catcaaaatc cctgttgccg gcggcggcac gtattcgccc gattttgcct atatcgtgaa
    12901 aaccaaaagc ggcgagatcc tgaactttgt gattgaagcc aaagggactg acggggcgga
    12961 agatttgcga aaaagcgaag agcggaaaat caaacatgcc gaaaagctgt ttgccgagat
    13021 ttccaaagaa atcaaggtgg tgttcaaaac gcagtttgac ggcgagagga tagccgaact
    13081 gatcgggcaa aatatgcccg caggcgggca ttctgaaaac gggcgttaaa gaacgaatgt
    13141 tcggggcgcg tgccgtctgc ttcgggattt t
//
LOCUS       DAWISC010000040        13109 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWISC010000040 DAWISC010000000
VERSION     DAWISC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.040"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            389..1105
                     /locus_tag="WIW87_000813"
     CDS             389..1105
                     /locus_tag="WIW87_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174003.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="WIW87_000814"
     CDS             1208..1633
                     /locus_tag="WIW87_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174004.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="WIW87_000815"
     CDS             1877..2077
                     /locus_tag="WIW87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174005.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="WIW87_000816"
     CDS             2145..2504
                     /locus_tag="WIW87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174006.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="WIW87_000817"
     CDS             2529..3383
                     /locus_tag="WIW87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ5174007.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="WIW87_000818"
     CDS             3453..3668
                     /locus_tag="WIW87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174008.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="WIW87_000819"
     CDS             3703..3963
                     /locus_tag="WIW87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174009.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="WIW87_000820"
     CDS             3960..4253
                     /locus_tag="WIW87_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174010.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="WIW87_000821"
     CDS             4256..4447
                     /locus_tag="WIW87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174011.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="WIW87_000822"
     CDS             4447..4587
                     /locus_tag="WIW87_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ5174012.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFQKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="WIW87_000823"
     CDS             4584..4793
                     /locus_tag="WIW87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ5174013.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="WIW87_000824"
     CDS             complement(4851..5768)
                     /locus_tag="WIW87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ5174014.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6637..6993)
                     /locus_tag="WIW87_000825"
     CDS             complement(6637..6993)
                     /locus_tag="WIW87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ5174015.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAI
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(7541..7921)
                     /locus_tag="WIW87_000826"
     CDS             complement(7541..7921)
                     /locus_tag="WIW87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5174016.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8034..9106)
                     /locus_tag="WIW87_000827"
                     /pseudo
     CDS             complement(8034..9106)
                     /locus_tag="WIW87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(9297..9386)
                     /locus_tag="WIW87_000828"
     tRNA            complement(9297..9386)
                     /locus_tag="WIW87_000828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9350..9352),aa:Ser,seq:cga)
     gene            complement(9462..9552)
                     /locus_tag="WIW87_000829"
     tRNA            complement(9462..9552)
                     /locus_tag="WIW87_000829"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9516..9518),aa:Ser,seq:tga)
     gene            complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WIW87_000830"
     CDS             complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WIW87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ5174017.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WIW87_000831"
     CDS             complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WIW87_000831"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ5174018.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLALARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WIW87_000832"
     CDS             complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WIW87_000832"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ5174019.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            12594..12959
                     /locus_tag="WIW87_000833"
                     /pseudo
     CDS             12594..12959
                     /locus_tag="WIW87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
BASE COUNT         3560 a         3367 c         3005 g         3177 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa ccttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca agaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagcccgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cctctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 tttccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cacaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc cgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 atggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaaag tcgtctgaaa tcgtgaacct tcagacgacc
     3421 tccacaaaaa cccaaatccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgagt aacctcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tacttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga aaggcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt ccagaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacaggttgc ggatttcggg cagcatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga ggcggctgcg tgtttccgcc
     4981 cagaccgtcc acatcgttac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tatctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggca atggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggccca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtaaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagggt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgcag
     6121 gggtgttgaa aacacgcaaa caggcgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatatagag ttatggaaat
     6301 gtgtcaagag aattagccca aatgggcgga cacgcggtta agaacccaca taggagggcg
     6361 gacttattcc cctttcgggt cttgtattcg tcgcccacgc ggtcatagaa acttcctgct
     6421 atcgaaacaa acaacaagga aagaaaacta tagacatgaa aaaatcacat tgacggagtg
     6481 attgccgctg tagtgtggtt tctgacgcca cgaacaggaa tataaaacaa aaccccctgc
     6541 acatgcaagg ggtttcccaa aaaaccgtag gcggcaaact gaaaggccgt ctgaatttca
     6601 gacggcctat gttgcggcgg atgttaagct attaagctat ttggttttca gaggcttttt
     6661 cttccatttt cgggctacgg cttcaaattc ttgaaaggcc gtcggtgcgc tcctgatacg
     6721 gcgtatttca aaaataaagc tgcttaggtt gtcccagtca cgtaacagca tggagtattc
     6781 gaaatcttta tacattttct cgtgcagtgc tccgcctaat acgcccgcac agacaaattc
     6841 gcgctggttt aaaactgtca gtatggcttc acggtctttc ttcttgtccg gggtatccga
     6901 tgtataggtg gcgagtatgc atccgtctgt ttttgccagc ccgtttacta tggttatggc
     6961 ttcttgaagg gcggcattgt tacgttcggc catgatcagc gcgctttttg gcgttttctg
     7021 tattcctcat gatgccgtaa gcggcaacaa aaacgccgat aacagtcaat atcggcgttg
     7081 ctatctgtat taggttgtca gtcattgccg tcccagccgt ctgaaaggcg gaaatcacgg
     7141 ctttccgaat aaatatgtcc cattgtcatc cccttaagct gatgttgaaa ataaatcctt
     7201 gcgtcggaat atacggcatt gcttgactat tttcaagatt ctgtacctcg tgaaccggac
     7261 tatacaaaaa aaccgccttc gtgtaaagcg gttcttaaat cgcccgttaa ggcggacaca
     7321 acgatagcca aactggaaca ggaggcggca aaggcgggtt tgtccaaaac agccttgatt
     7381 gaaaggctga ttggggattt gtaggcgttt gcagatacga caaggggcgc attccgcgcc
     7441 ccatcacacg cacggacggc ttgtcatcgc ctgtcgcccg tgcgcggctg cccatatttc
     7501 agaacagtct tgcaagcccc tttaagggaa cggttttatt ctagtacagt ttgaatgcct
     7561 tggcaacggc tgttttaatc gcctgaaaat cctcttcact gattattgga atgcagcggt
     7621 cgcgcccttt gggtttgtat cggtctaatc gtgccaatcc gactgttgcc gtcatgtcgc
     7681 attttgccca acattggatg tgcggcttgt ccggtaaggg gtttccactc attttgtggt
     7741 ggtagtccgc caaagggaca ggttctgtgc tgcttaaggg tacgaccgtt accagtttgc
     7801 cgttgtgcct gtttcgcgct atgacgacga cagggcgttt cttgaccatt tccggttctt
     7861 cataaccgcg aaagtcgcac atgataaccg aacgttccct tggttggaat tttaaaggca
     7921 ttagccgctc ctgacgatga cgggctgcta ttatagcagt tcttacacaa aaaaccgcct
     7981 ttgtgtaaag cggttgcaaa aaagccttcc aataaaaatg cggaaacggt tttttatttg
     8041 cgtttccgca taatactatc caccttcaga acctcgcgca aataagtacg cgcaagcgag
     8101 cgcaaaccgt gcgccgtcgt atccagcccc gcccgctgca tcgccacgcg cggactttcc
     8161 ttattgatat gcccttgaaa attcacactt tcaaacacaa accgcccgtt cacattcaac
     8221 gcccgcgcct gatccaacac ccactgcatc gcccgcgaca gcggcacatc atgcccccgc
     8281 cgctcctttt tccgttcggc ggggatacgc caaacctccc cgtccaactc cgaccactcc
     8341 atcaacgccg cctcctgaat ccgcgtcatt gtcaacagca gccaatagat acaaagccgc
     8401 gttacaggat ggatacgcac cctgcccgca taaaccgcaa attcattgcg ctgctccaaa
     8461 aaatcaatca ggcgcggcaa ttcagacgga ctcaacgctg ccatattccc cgttttcgcc
     8521 cgttcaaaca cacccgaacc gatttgcgcg acagggttga tttccatcat tcccgaaccg
     8581 accgcaaacg caaacaccat cttcagactg tttttcgttt tgcgcaacgt atcaacgata
     8641 ccgcgcgcct ccatcacacg cagacagccg accacatccg ccgtcctgat ttgacgaata
     8701 tcaagattgc cgatagccgg aaaaacccac cgctcaaaat tccgcataac ctgtccggca
     8761 tacttttcag accgcccctt cgaccaacgc acaaaccaat cacgcgccac cttctcaaaa
     8821 gcaaaatccg cccgcacctt cttattgacg acattttccc cgtgcgcccg ttttcggcgc
     8881 acctcctccc gccattcccg cgcatcggcc agcgaaaaat caggataccg ccccagcgaa
     8941 attgtctgct gccttccatc ctgcacaaac gacagcttcc aactccgccc gcccgtcggg
     9001 tagacccaca aagccagccc gccccgtctg acaacttata cagcttatcg cgcggcttcg
     9061 catttttaac ctgatttgcc gacagcggcg taatgatttt cgccattatg gtaatttccc
     9121 atttatccaa aaattaccat acacattacc ataaaaaaat gcaataagaa gatgccgcaa
     9181 gattgcaaat gatttgcttt gatgcgttat gatagggcaa accgatgatt tttatatggt
     9241 aattgataca acttgaacaa aaaagaaccg ccccgaatca gggcggtttt gttttgtggc
     9301 ggaaacggtg ggattcgaac ccacggagga tttgcaccct cagcggattt cgagtccgct
     9361 gcattcagcc tctctgccac gtttccgata atgcagtaaa accaaataaa aatacaatat
     9421 ttgcggctat tgcattctta tttggtttta cgctcaaaaa ttggcggaag cggtgagatt
     9481 cgaactcacg gagggctatc aaccctcgac ggttttcaag accgttgcat taaaccactc
     9541 tgccacgctt ccgtcttctt gaagattagg aataataatg aaatttatac attttgccaa
     9601 gcactttttt cggaaaatac ttaatttatt gttttcactt tatttgttcc gaacgcataa
     9661 aaacccattc gctttcattt gaagccgcgt cattgaatgc gtagccttcg taattaaaat
     9721 ttttcagcat ttcagggtcg gtaatatgat gttctgccgc atagcgcacc attaatccac
     9781 gcgcgcgctt ggcatagaaa ctgatgattt tatatttacc gtttttttcg tctttaaata
     9841 ttggggtaat cagccgcgcc cgaagttttt tcgtgttgac ggacttgaaa tattcctgtg
     9901 aggcaaggtt gacaagcgta ttgctgcctg cttgggcaag cgtatcattt aaaaggttgg
     9961 taatgatgtc gccccaaaac tcatacaaat tcttgccgcg caaattggca aatgccgtcc
    10021 ccatttccaa acgatagggc tgtatcaggt ctaacgggcg aagaagaccg tacagaccgg
    10081 acagcaggcg gacatggttt tgcagatagc gtatctgtcc aatatccaat gtgtttgcat
    10141 ccataccttc gtaaacatcg ccgttgaaca taaagaccgc ctgtttggcg ttttccggcg
    10201 taaacggcgt gttccattct gcattgcgct gcgcgtttaa gagggcaatt ttgtcggaaa
    10261 cgtgcatcag ttcggcaatc tgttgcggcg caagctcgcg caactgctgc attagaatgt
    10321 cggactctgc cagcaggtcg ggttgggtaa actcgctgac aggggcaggg tctttttcat
    10381 taaggttctt cgcaggggaa aggacaaaaa acataatctg ctcggtattg tgtcaaacac
    10441 ggggatttta agtttaaacg tgtttgcggg caagccgatt aaaagtgaag tatagtggat
    10501 taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatctgtact gtcttcggct
    10561 tcgtcgcctt gtcctgattt ttgttaatcc actataaata ccgttatagc aaattcaacc
    10621 gtaaaatatt gaaaaatgcc gtctgaacaa aagttcagac ggcatttttt tattctttac
    10681 cgatataggg aaacaatggt ttccgcagct ttgacacaag ttgcgacatc ggcaaccaaa
    10741 gcgatacgca cataaccttc cccgggattg ccctgttcgg tatcccgcgc caaaaaacgt
    10801 ccgggcaata cttggatagc cgctttttgc cataaattgc gtgcaagtgc caaatcgtcg
    10861 ccatcaggga ctttcaacca gatgtaaaac gaggcatccg gtaatttaac gtcaaatacc
    10921 tgttgcaaaa tgggaataac gcgctcaaat ttttcctgat acatacggcg gttgtcgata
    10981 acgtgctgtt catcatccca agcggcaatg cttgcgcgct gcacgggaat actcattgca
    11041 ctgccgtgat aggttctgta aagcagaaag tttttaagca gttcggcatc gccggcgaca
    11101 aaaccggaac gcaggcccgg aacgttggaa cgcttggaca aactggtgaa cataagcagt
    11161 ttttgcctgc ttcgacccaa ctgtgcagcg gcttgcaggc agcccaaagg tttgttgccg
    11221 tcgaaataga tttcggaata gcattcatcc gaggcaataa tgaaaccata tttatcctgt
    11281 aaatcaaaaa cttctttcca gccgtccaaa tccagcacgc tgccgctggg gttgttgggc
    11341 gagcagacga acaccagttt ggtgcgtttc caaacctctt cggaaatact gcgccaatcg
    11401 gggttgaaag acggcgcggg gcaattggca aaatggattt caccgccgcc caaaagtgtc
    11461 gcaccttcgt aaatctgata aaagggattc gggctgacaa ttgcgggttt gatgccgtct
    11521 gaaacagggt tcaacacggt ttgaacaaaa gaaaacaacg cctccctact gcctaaaacc
    11581 ggcagaattt cattatccgc atccactgtc aagccatcgt aacggcgttt taaccagttt
    11641 gcacacgcct gacgcagttc aggcagaccg gccgtcagcg gatatttttc caactcgtgc
    11701 aatgaggcgg tcagcgcatc cgtaatgact ttcggtgtcg gatgtttcgg ttcgccaatg
    11761 tgcaggggga cggcttccag accttcgggc gcggaaatgc cctgcatcgc ttcacgcagt
    11821 cgggcaaagg gatatggttt gagttggttg agtaaggtat tcatgatttt ctccggttgt
    11881 cggtatatcg gaagatatgc cgtctgaacc tgacgtgtct ttcagacggc attctatatt
    11941 tttagtgaga acccgtactg ccgaagccgc cctcaccccg gctgcttccg acaaactcct
    12001 cgacacgttt gaagcccgcc tgcacgattg gcacgacaac catctgcgcg atacgctcaa
    12061 acggtttgac agtaaacggt tcgctgcttc tgttccataa cgacaccttc aattcccctt
    12121 gataatcgga gtcaatcaaa ccgaccaaat tgcccaaaac aatgccgtgt ttatgcccca
    12181 agccggaacg gggcagcaaa acggcggcat atgcgggatt cgccaaataa attgccaaac
    12241 ccgtcggcac aagaaacgtt tcacccggct gcaaaacgac ttcctcatcc aaacaggcgc
    12301 gcaaatctaa acctgcagaa ccctccgttg catagacagg gacaacatcc gccatccgtt
    12361 cgtccaatac tttcatttct acttcaatat tcatcgtctt gctcctgcat acggccggtt
    12421 tcaaagatgg gagattatac acaagccgtg tttacgctga aacacggcaa atcggttttg
    12481 ttttgaaatc cgttgccagt gtataattcc cgcccttccg gttatgaaga cgaacattat
    12541 gcacaccttc cccgcataac gaatttatac gcggcatcaa agaaagttcg cctatgctga
    12601 ttgggctgct gccttgggca ttaatactcg gtatgcaggg cggacaaaaa ggcatgagct
    12661 ggctggaaat gttgttgatg accagtatga acttcgccgg cggctccgag tttgccacgg
    12721 tcaacctgtg ggcggaacct ctgccgatac tgcttatcgc caccgtaacc tttatgatta
    12781 attctcggca tatcctgatg ggggcggcgc ttgccccgca cctgaaagga ataccgctga
    12841 aaaaagccgt gcccgcactg ttttttatgt gtgatgaaag ctgggcgatg gcattctccg
    12901 aaatccaaaa acggaaagca gccggtttgc ccgcattcaa tatgcctttt tatagtggat
    12961 taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gaaaacgatt ctctaaggtg
    13021 ctgaagcacc aagtgaatcg attccgtact atctgtactg tctgcagctt cgccgccttg
    13081 tcctgatttt tgttaatcca ctatatgaa
//
LOCUS       DAWISC010000041        12910 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWISC010000041 DAWISC010000000
VERSION     DAWISC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.041"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(91..465)
                     /locus_tag="WIW87_000834"
     CDS             complement(91..465)
                     /locus_tag="WIW87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174020.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(638..994)
                     /locus_tag="WIW87_000835"
     CDS             complement(638..994)
                     /locus_tag="WIW87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ5174021.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1137..1481)
                     /locus_tag="WIW87_000836"
     CDS             complement(1137..1481)
                     /locus_tag="WIW87_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174022.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1640..2041)
                     /locus_tag="WIW87_000837"
     CDS             complement(1640..2041)
                     /locus_tag="WIW87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174023.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2045..2785)
                     /locus_tag="WIW87_000838"
                     /pseudo
     CDS             complement(2045..2785)
                     /locus_tag="WIW87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2855..3172)
                     /locus_tag="WIW87_000839"
     CDS             complement(2855..3172)
                     /locus_tag="WIW87_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174024.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3169..3771)
                     /locus_tag="WIW87_000840"
                     /pseudo
     CDS             complement(3169..3771)
                     /locus_tag="WIW87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4197..4541)
                     /locus_tag="WIW87_000841"
     CDS             complement(4197..4541)
                     /locus_tag="WIW87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174025.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4579..4923)
                     /locus_tag="WIW87_000842"
     CDS             complement(4579..4923)
                     /locus_tag="WIW87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174026.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(4926..5114)
                     /locus_tag="WIW87_000843"
     CDS             complement(4926..5114)
                     /locus_tag="WIW87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ5174027.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(5184..5519)
                     /locus_tag="WIW87_000844"
                     /pseudo
     CDS             complement(5184..5519)
                     /locus_tag="WIW87_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5625..5996)
                     /locus_tag="WIW87_000845"
     CDS             complement(5625..5996)
                     /locus_tag="WIW87_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ5174028.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(5993..6244)
                     /locus_tag="WIW87_000846"
     CDS             complement(5993..6244)
                     /locus_tag="WIW87_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174029.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(6376..6582)
                     /locus_tag="WIW87_000847"
     CDS             complement(6376..6582)
                     /locus_tag="WIW87_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174030.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(6592..7437)
                     /gene="mafB"
                     /locus_tag="WIW87_000848"
                     /pseudo
     CDS             complement(6592..7437)
                     /gene="mafB"
                     /locus_tag="WIW87_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(7534..7746)
                     /locus_tag="WIW87_000849"
     CDS             complement(7534..7746)
                     /locus_tag="WIW87_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174031.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(7822..8181)
                     /locus_tag="WIW87_000850"
     CDS             complement(7822..8181)
                     /locus_tag="WIW87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ5174032.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(8186..8902)
                     /locus_tag="WIW87_000851"
                     /pseudo
     CDS             complement(8186..8902)
                     /locus_tag="WIW87_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(9119..9502)
                     /locus_tag="WIW87_000852"
     CDS             complement(9119..9502)
                     /locus_tag="WIW87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174033.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(9468..10913)
                     /gene="mafB"
                     /locus_tag="WIW87_000853"
     CDS             complement(9468..10913)
                     /gene="mafB"
                     /locus_tag="WIW87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ5174034.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(10953..11915)
                     /gene="mafA"
                     /locus_tag="WIW87_000854"
     CDS             complement(10953..11915)
                     /gene="mafA"
                     /locus_tag="WIW87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ5174035.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(12099..12818)
                     /locus_tag="WIW87_000855"
     CDS             complement(12099..12818)
                     /locus_tag="WIW87_000855"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ5174036.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
BASE COUNT         3539 a         2636 c         2300 g         4435 t
ORIGIN      
        1 ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
       61 gggcagcctc atctctttat agatagctgc ttaccaagta tccttaattg ctaattcaag
      121 ccaatcttca aaactattaa aaaattcatg gttttctata ttatctaaat taatgacaat
      181 aaccctagaa tttcctgtta catcattacc atcaaaataa gcttcccatt ctccattact
      241 agcgaatggg ataagattta aatcaggcag gatagaattt ctaaatatat aagctacttc
      301 tgccccttct gttccataat tctcaaacca ccagtgaaaa ttatgtggct gaatataagg
      361 tattaaagat gtatcgttag agatacgaac atacttttca ggataacaaa acctttcagg
      421 aagcatatta ctaggatata aattaaattc attttgaata atcatttcgt taccttagga
      481 tcataaatac taccattagg aaactgggta gaccaatttg gtggtgctag ttcattttca
      541 atttgttttt taattctaaa ttgaatagct tctccccatg tttgcggcat ctaatgctgc
      601 ttgctctgca tcaatattac taaaaattgg attagtctca aaaatgaaaa attactgttt
      661 tttcattttt tatagcatat tcaagtacat gaataatatt tttcactaag ggctctgtag
      721 aaaaatctga attttgtaat ttttctttta cagccaaaat actttcctta tctattagcc
      781 aatccccctc acttggtcca aataaccaat tatcggtaat ttcagaaaca agtctccata
      841 atccacgatt ttgatattca ataatttgat tttccgtaat ttctacaact gtttcagatt
      901 gaatgttttt ataagcccaa tcaatcattt cagtatattc actaattgaa acattgtctt
      961 ttaaacagaa aattaacagg ttgtgttcat tcatttgtct aagttcctga tatgagtttg
     1021 ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta aaggattaac cgcaccttgg
     1081 atgatgccgc gaatatcatc catacggtta ccccaccaat ttttattggt gtatcgttaa
     1141 tcatttattt caaaaaagtt atttattatc atattatcag ggagcaggtc gttaaaaaaa
     1201 accccctcaa attccattaa attagtattt atccagcaca taaacgaacc aaagcgttct
     1261 ttccctgagt gtaaatattt aaaattaaac tcaaaataag tttttttgct tagaaaattt
     1321 aatggttttt ctatagtgaa aaaaaagccc ttactcgttt tcgttcgaga tactatttta
     1381 aataaatttt tattttcaat aaaataagaa tattcattta ctaagttgtt atgataaaaa
     1441 agtaatttta ttaaaatgtt ttcataattt tctaggttca tttctagctc aatttaaaat
     1501 gatttcattg ttgccgtacc aatgcgaaat ttaccatcaa caaaatttcc cttcactgat
     1561 acaggtacac catcaacttg ggtagtttta ttttcaggct gcctgaaaga aagataggca
     1621 acttattact catcttacgc tagccctcaa taattgaata cgttgcacta gaaccaatta
     1681 tttctatgaa tgtttgctca aacataaaaa tatagtattt aaagaattct ctattcaaag
     1741 gaaaataact ttctttcaat atccattccg cataacactc atcaagaagt tcaaaaaaag
     1801 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
     1861 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
     1921 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
     1981 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
     2041 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
     2101 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ccatcaggta
     2161 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
     2221 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
     2281 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcgcct
     2341 taccggcttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
     2401 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
     2461 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
     2521 agccggtatt tttaacccaa tcccatttgg tcgggttaag ttctactttt ttacctctcc
     2581 aaaccgtacc tgcggcctct gctacggcaa gggcagtttg ggctgttgct gttatattcg
     2641 gatgggcatc agcccattgt ctggcggaat tgatgccgtc ttttaccgca aaagcactgt
     2701 cctgtaatat gctcgcggca gcaagttgtg cttccgggct taattttcct gaattattaa
     2761 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
     2821 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
     2881 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
     2941 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
     3001 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
     3061 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
     3121 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
     3181 atatccagaa tgatgatgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
     3241 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
     3301 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagccgcatc
     3361 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
     3421 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
     3481 catagggcgg gcagcaggtg ttttatagcc gttattttta acccaatccc atttagtcgg
     3541 gttaagttct acttttttac ctctccaaac cgtacctgcg gcctctgcta cggcaagggc
     3601 agtttgggca gtagctgtaa tgttcggatg ggcatcagcc cattgtctgg cggaattgat
     3661 gccgtctttt accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc
     3721 cggacttaat tttcctaaat cattaatacc ttgtaaagtt tgctgcgcag agatgtaggt
     3781 cggatatttg tatccgacaa aaatatttga cgcatctatc gtttccgaaa tgccgctgtt
     3841 ggaaaatgtc ggattcgaga atccgaccta cggctatttc gtttaagtca taaatagcca
     3901 ttcatgttcc ttctgaattt gcttttgcta tccttagaac caatcatata aacattatca
     3961 tcattaatta atgttctaag tttatgaagc ggtcaaatct gtcaaactac cagagatgaa
     4021 gcatatcctc ctggaagtaa ttctgttcgt gctgtttcca tatcttcttt gccatcatca
     4081 gttacttctc tgtctaaata aaatcttacg ataagttttt gtctaatttc atcaaattct
     4141 gccgtaatta atcttatatt gatatgaata cacggttcat acctgctatt agccagtcag
     4201 gtgtagttgg atggggattc atttttaata atatcttcaa gcaagtgaaa tgtaagaaaa
     4261 gcgtagatac ttgaaggccg gctatctgat tggcaaaaag aaatttcgga gataaagaat
     4321 ttccgtttat atttttcata aattaaatca acaccttgtt ttacataaaa actaacttcc
     4381 tcaggaagga tatttataac attctctcct tttactttta tagttaatgg tttgattttt
     4441 atgttttcat gaatatctga taggattatt tctaaaaaat tatttttatt tccagtcatt
     4501 ctggctattt tatgtatatt tccattgtgt tcgattatca ttttattttt tccaaggatt
     4561 tccttctata ttgatagttt atctaaagat ttcatttaaa atatcctgac taatttcttg
     4621 tttatataat tctacaattt tctgtttctg tgtatttgta taaaattttt cgttggtatc
     4681 tgcttgatat tttaattgtc tcaatatttc catgatttca tattctgatt tttcatcata
     4741 ttcttcaatc atgtaatcaa caaattgttt tagaatatca aaggcttcgt aactttccaa
     4801 aattttaata tgtttatcgg caaaattgga catataccat tcgtcagtga ttggattagt
     4861 atttaaatca tctaaaaaaa catttaaatc atctaaaaaa acatttacat catctaaaaa
     4921 catgactaat ccttaaactt aaaatctgtt ttctcaccgg ttcttgagtt ttcaatgtaa
     4981 tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc aaccgtcttt tgctaaccaa
     5041 ccatttttag tatcggacat aggaggtatt ctcgcaggag ttttgccctg tgggtttgcc
     5101 ttaacttctt ccattgctaa ttgttccttt aaattattag gtattgtacg accggttgat
     5161 ttttcaacag attgtgtccc tatttttaat gatttgtttc ccccagccat ttccccatct
     5221 aaagtctgca tagggcgggc agcaggtttt ttataaccgg tatttttaac ccaatcccat
     5281 ttggtcgggt taaggtttac ttttttaccg ccccaaaccg tacctgcggc ctctgctatg
     5341 gcaagagcag tttgggctgt tgcagttata ttcggatggg catcagccca ttgtctggcg
     5401 gaattaatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcgc ggcagcaagt
     5461 tgtgcttccg gacttaaatt tcctaattca ttaatatctt gtaaagtttg ctgcgcagag
     5521 atgtaggtcg gatatttgta tccgacaaaa atatttgacg catctatcgt ttccgaaatg
     5581 ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctgctacca accatcattt
     5641 tcagcttctt ttagccaagt atcaaaattt tcaaaagtct cgtaatattt tgtattatct
     5701 aaattaacta ctataatttt aggattacct gatttatctg aaatatcaaa acaggcagcc
     5761 cactcttggt ttctagcaaa aggtaatagg tttggaatgc cagttatttc aaaataaatg
     5821 tcaattactt ctgatatatt gctttctgca ttttcaaacc accaaggaaa agaatattgt
     5881 tcatcgtagt taatggcatg agtggattga gctaatttta aataactttg cggatattta
     5941 aatcctttag gaagcattgc ttcaggatac ggaacaaaac tattttgttt tatcatttcc
     6001 taagtacctt tacatcataa atacttccgt taggaaatct tttgctccac ccttctggaa
     6061 ctcctttttg tgtttcttgc tttctaattc gtagctggat agcttctccc caagtttgag
     6121 gctcaatacc atcatgctta aacctgattc tctcttttga tgataatcgg tcattaaaat
     6181 tatgaacatt cgcttccact tttactcctg cttcacgtgc tgccataata cgcgtgttat
     6241 ccatactagt aggtgctcca tcaggcatat taacaacatc aaccctgtcg ccaacccatg
     6301 atttttttct ttcatttcgg ctacaataga atcatagttg taatttgcac taggctctga
     6361 caaaaatttg ggttgttaag ttgtcatttt tttataaatt ccagcaaact gttgttgtaa
     6421 attatctact tcatgttttc taacaccaga tacattttca aattctcttg aatctacaca
     6481 tacgcccgca tatatctcat tcaagatagc tcgtaaaaca aaaacttcat cttgagttag
     6541 tgaaatagtt gagttttgat tatttatgga tagtatattc atggcttttt cctataaatc
     6601 atcataatat tttttacctg atgttggtct aaatgctgta cctccatcgt cagaattttt
     6661 atctcggata actattgtcc ctgttttatc atcccaatac gcagttctac cgcgaggtaa
     6721 ctctttcata tttgttggat ggctaacaat attttcaata tgccgagcaa aatctgctgg
     6781 tgaattgata tttaaatccg taaattcttg ttgtcttatg acatgcttgt tataagcatg
     6841 cccactacta atttgttctc caattagttg cgcttgtaaa gacggttgtg tggaagcatc
     6901 tgctttgctg ttggacgtta tagatttagg cggtctattc ccaccggcca tttccccatc
     6961 caaagtatgc atggtgcgaa cagcaggtgt tttatagccg gtatttttaa cccaatccca
     7021 tttggtcggg ttaagttcta cttttttacc gccccaaacc gtagttgcgg cctctgctac
     7081 ggcaagggca gtttgggctg ttgctgttat attcggatgg gcatcagccc attgtctggc
     7141 ggaattaatg ccgtctttta ccgcaaaggc actgtcctgt aataggctcg cggcagcaag
     7201 ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct gctgagcggc
     7261 tgtatctgtg acagggctta ccgcactgtc tgtaattgcc ccaatcccta ctccttgaaa
     7321 acccattaaa aaaggattaa ccgcaccttg gacgatgccg cgaatatcat ccatacggtt
     7381 agcccaccaa tttttattgg ggtcgttttc ccatatcagt tttcctgctt catcagcaca
     7441 gttaggattg gcattgatgc ccacccagtt tcaggcagcc tgaaaaggat ttttaatata
     7501 cggcaaaagg tgggcaacaa gtgcccacct tacctagcta acatttatcc caagtatcat
     7561 atattttttt aagttcttta tccaaagaaa gaactttatt ttttcaacac catgaattat
     7621 ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt tctcctacaa ttgcttgaat
     7681 aatatacaac tcatctttat cgagactaat tttaactgaa ctctcttcaa aattaattaa
     7741 tttcatgaat aattcctttt attttaattc gtcatcaaaa taccgtttgc ctgtttttgg
     7801 tctaaatgct gtccaccatc tttagtcttg gatatttaga ttataatcgt caaattccca
     7861 gtcaaaatat tttgccaaat ttgatataga cttatcttca gacgttgaat tacatatttc
     7921 caatatatca tttatcaatt ttaatatgtg ttcaatatta aaacggtttg taacaattga
     7981 gttttttaat gacatcggtc tatgtatatt tgaaagtaac cattcagtgc tatatatata
     8041 aacataaaaa taatcagcac cattactttc atcaaaacct attgataatg tcaaaagtat
     8101 acaaaaattt tctaacttgt cttgtgggaa atcttccaaa tcaaagtcag aacaatcata
     8161 attttttaat tttaatctca tataattacc taatatttaa atgtccattt ttaattttat
     8221 tcctttttcc atttgaatca atagtataag tttcaaaatt tgcttgaata cctgtagttg
     8281 caaattgtga atttttttct gttggtggcc gatattgtcg agtgccatct atacttaacc
     8341 atccgcctcc actagtttgt cttgcaccct caccaaccca aattttacct agcctatctg
     8401 cctcttcata agttgcagtt cctattggaa aatttttttc actcttatga agagctagac
     8461 tcaatcttgg atcttgagcc gcaatgtttt ttaagttttg cccagttaat tgattaacta
     8521 attgaggata agttgcagca ttggcttttc ctccagacgt tatagattta ggcggtttat
     8581 tcccaccggc catttcccca tctaaagtct gcataggtcg ggcagcaggt gttttataat
     8641 cggtattttt aacccaatcc catttagtcg ggttaagttc tactttttta ccgccccaaa
     8701 ccgtacctgc ggcctctgct atggcaaggg cagtttgggc tgttgcagtt atattcggat
     8761 gggcatcagc ccattgtctg gcagagttga tactgtcttt taccgcaaaa gcactgtcct
     8821 gtaataggct cacggcggca agttgtgctt ccggacttaa atttcctaaa tcattaatac
     8881 cttgtagagt ctgctgcgca gagatgtagg tcggatattt gtatccgaca aaaatatttg
     8941 acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag aatctgacct
     9001 acggctgctg actattttct tgtttatata attctacaac tgcaaactac tatcaaatct
     9061 ttttatctct ggctctacac tcagcaagaa tatttttatt ttacaaaaca taggctagtt
     9121 atttgagtat attgtcaaat tctaatttgt ttttttgata gatttttttt ataccattaa
     9181 tacattcatt agttgtaatt ctaccaagcg aatgtaattt atcaatatca aaacaatcgg
     9241 tataaaccct attcacagtt ttatcttgta tttccttatg aatacttaat atattgagat
     9301 agtttagaaa ttttattttc tcagattgag gtaactcatc aaaaccaagc caataataaa
     9361 aattgttata tgattttaaa aaatcaacaa tttttatttt tcctaaaacc aaatcttcaa
     9421 ctaactgggt tgcataaata atatcattta tgattttaac aggtaaatta tttgatgggt
     9481 aaccatgggg ttccgtcatc atttgtaact cctggtacat gtgcgtgtcc tgtaagtgcg
     9541 cgtgggtctt gatgggtatt tgcatgatga gcatcatcta ttctggctcc agtcaagttt
     9601 ccattactat ctcggacttg gataaaacga gcatcaggcg aacctgttat ataaccgcct
     9661 attggtgttg gtattgcatt tcctttgctg tctacataaa ttgttcctgt cggagaaata
     9721 gatgtatcag gtttaacata tggggcaggt aagcctatat tgttatcaga gtgtgtagag
     9781 tgttgctgcg tacttggttt cggtggctta tttcccccag ccatctcccc gtctacagtc
     9841 tgcataaggc gaacagcagg ttttttatag ccgttatttt taacccaatc ccatttggtc
     9901 gggttaagtt ctactttttt acctctccaa accgtacctg cagcctctgc tacggaaagg
     9961 gcagtttggg cagtagctgt aatgttcgga tgggcatcag cccattgtct ggcagagttg
    10021 atactgtctt ttaccgcaaa agcactgtct tgtaatgcgg ttgcggccgc aagttgtgct
    10081 tccggactta aatttcctaa attgtgaata ccctgtaaag ttttccgtgc tgccgcatag
    10141 gttaccgggt ttaccgcact gtctgtaatt gccccaactc ccaatccctg aaaacccatt
    10201 aaaaaaggat taaccgcacc ttggacgatg ccgcgaatat catccatacg gttagcccac
    10261 caatttttat tggggtcgct ttcccatatc agttttcctg cttcatccgc acggctgaaa
    10321 aaaccagagg cggcaccggc attttctttt agccaacggt ctgaaaatgg ggcttgaggg
    10381 acaatattag tctttgtttt tgttgaagtt cctttgacat aatagctgta tatatccctt
    10441 gctcctccgg ggggcggata atcgctgcct tgcggcccgt catatccatc ctccggatga
    10501 atttccgacc ctgtccgatg aagttggtaa acagtaaaac caccgtctac accgccgctg
    10561 aaatcagaag tgctttttga atcatgatga tcgaacggac tgtgtacttc atgtccatgt
    10621 cctgaaaaat gggtttcata accgataatg ccttcaaacc ctgtccgttc gtgtgtaata
    10681 ggcagtacag gactgaccgc agttgcatca aatgtctgga cggcgtaaac ccgattttta
    10741 acactgccgc gagcattacc aaacagatgg tatttaccac ccggttcgta gtgttttgcc
    10801 tgcatatcat cgcgtaaacg ggcatccaaa ccgttcgcat gactaatggg gatttgcagc
    10861 aacgatattg ccgctgcaaa cagcatcatg aatttttgaa taggcaattt cattttgttt
    10921 tccttggtag caagcggtta tggtcgtgtg aatcaaggtt gcccttgtct atgttgtcgc
    10981 actacttcat cgctgtatcc atacccctca tgactgttat cagtctctac ggatggggcg
    11041 gagttacccg tatgattgcc gtatggtcgg atatcggaga aatcgaccat taatccttcc
    11101 gtcggtttaa ttcctttgct tactttatac ggccccatcc acaatgcgta attttcttta
    11161 taggcagctt caaacgcatt ggttttgggt ttgatgagca attttttatt ggttctgtct
    11221 actgcgaaat attccagttt tgtttgggct ttcagtgttt cggcattgta taggtgcatt
    11281 tcggttctgt tgcgtatcgt tccgaatacg tcgatgttaa taaacacatc tgtatcggca
    11341 ttggcaggag aaacaacgtc tatgccgcgc aggaaaaata cggtctgtac caagtgggaa
    11401 agaaaggcag tgtcgcgcgg gttagtcgtc aaggtttcat ttcgataatc ccccatcccg
    11461 ccaatattta agcccagact gctcctactt ccgctaccgt ctgattgggt gcgcgagcgt
    11521 gcaggggcat taagtgtaga taaagaagtg gttaaacctg tcaaaccgcc tgatgttgtt
    11581 tcagcggtgg tttcgtaacg tggataggtg taatcggtac ggacggcagg gctgtttatg
    11641 tattcgccac gaatcagtgc atcaatggag tagcgacccc ctgtcaaact gcctgaacct
    11701 tggtcgccca tagttgcaat gtacaatgca acttttcgtc cgtgtaatgc ctgtaaatcc
    11761 atgtctttaa cggcagctct ggcagaagcg gccacaagtt cttgttcgac cgcaaagcgt
    11821 ttacctccgc catgcgatgg aatacctgtc agtgtcccgc aggcggataa aataaaaact
    11881 gaaaaaagaa taggtatcag cagccgtgct tgcatagatt ttctcctttg atgaaaaaca
    11941 aattgtatca aaattgtaaa tatagtggat taaatttaaa ccagtacggc aaggcgaggc
    12001 aacgccgtac tggtttaaat ttaatccact ataatctcca tataataaat ggctgtctga
    12061 agcccatatg cgactatatc tgccgacact atggtcaatc agcagtgaac cagcgtacct
    12121 tcgttttcac cggttactac acgtttgagc gagccttctt tagcaatgcc gaatactaca
    12181 atattgagct tgcgttcgcg gcagagggcg aaagcggtgg catccatcac tttgaggttt
    12241 ttcaacaagg cttcgtcaaa agtaatggtt tcgtagcgcg tggcggatga gtcttttttg
    12301 gggtctgcgg tgtacacgcc gtcaacgttg gtggctttga gcatcacgtc gcagttcatt
    12361 tccgcaccgc gcaatgcggc ggcagtgtcg gtcgtgaaga acgggttacc ggtaccggcg
    12421 gcaaaaatca cgactttgcc ttcttccaaa tattggatgg ctttaggacg ggcgtaagtt
    12481 tcagcgattt gctgcataga cagtgcggat tgtacgcgcg ctttgatgcc taaagtttca
    12541 aatgcgtctt tgagtgccaa cgcgttcatc acggtcgcca tcatgcccat gtagtcggcg
    12601 gtggcgcgat ccatgctgcc tgcttgggcg gatacgcccc ggaaaatatt gccgccgccg
    12661 acaacaatac cgacttgcac gcccatttta acgacttcgg caatttcgcc gacagtttga
    12721 acgatggtat cgtgattgat gccgaacgga tcggaaccca tcagggattc gccggagagt
    12781 ttcagtaata cgcgtttgta tttgatttgc tgtgtcatgg gataccttgc tttcttgaat
    12841 gtcgtcggtc ggagtttggg ccgtctgaat tttcaaacgg tcggaattac aatgatatgg
    12901 tgtgtcgttc
//
LOCUS       DAWISC010000042        12762 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWISC010000042 DAWISC010000000
VERSION     DAWISC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.042"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..325
                     /locus_tag="WIW87_000856"
     CDS             <1..325
                     /locus_tag="WIW87_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174037.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(497..913)
                     /gene="gloA"
                     /locus_tag="WIW87_000857"
     CDS             complement(497..913)
                     /gene="gloA"
                     /locus_tag="WIW87_000857"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ5174038.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(938..2107)
                     /gene="lapB"
                     /locus_tag="WIW87_000858"
     CDS             complement(938..2107)
                     /gene="lapB"
                     /locus_tag="WIW87_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ5174039.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2118..2432)
                     /locus_tag="WIW87_000859"
     CDS             complement(2118..2432)
                     /locus_tag="WIW87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ5174040.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2742..3740
                     /gene="ilvE"
                     /locus_tag="WIW87_000860"
     CDS             2742..3740
                     /gene="ilvE"
                     /locus_tag="WIW87_000860"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ5174041.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETEAYLDALIK
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="WIW87_000861"
     CDS             complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="WIW87_000861"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ5174042.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="WIW87_000862"
     CDS             complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="WIW87_000862"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ5174043.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5955..7598)
                     /gene="pgi"
                     /locus_tag="WIW87_000863"
     CDS             complement(5955..7598)
                     /gene="pgi"
                     /locus_tag="WIW87_000863"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ5174044.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALKFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFSTPTRHNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            7955..9187
                     /gene="pilG"
                     /locus_tag="WIW87_000864"
     CDS             7955..9187
                     /gene="pilG"
                     /locus_tag="WIW87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ5174045.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9261..10121
                     /locus_tag="WIW87_000865"
     CDS             9261..10121
                     /locus_tag="WIW87_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ5174046.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10123..10755
                     /locus_tag="WIW87_000866"
     CDS             10123..10755
                     /locus_tag="WIW87_000866"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ5174047.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            10748..10957
                     /locus_tag="WIW87_000867"
     CDS             10748..10957
                     /locus_tag="WIW87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ5174048.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11042..12718)
                     /gene="pilB"
                     /locus_tag="WIW87_000868"
     CDS             complement(11042..12718)
                     /gene="pilB"
                     /locus_tag="WIW87_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ5174049.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2929 a         3547 c         3331 g         2955 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttg ccttatcggt gtaacggata aagtttgaac cggcacaggc
      421 tcaaacagca ggtcgacggc aacaaaatgc cgtctgaaac ccctaaaggc ttcagacggc
      481 attggcggcg gcggtatcag gcagtttgat aggcaaccga atcgtcgccg cttttctttt
      541 gaatgaactc gattttgtat ccgtcggggt cttcgacgaa ggctatcacg gttgtgccgt
      601 gtttcatcgg gccggcttcg cggacgacgt ttccgccctg ccgcttcaca cgttcgcagg
      661 cttcgtaggc atcgtccact tcaaccgcga tgtgtccgta ggcgttgccc aagtcgtatc
      721 gttccgtatc ccagttgtgc gtcagttcca aaaccgtgct gtcggtttca tcgccgtaac
      781 cgacgaaggc aagggtaaat ctgccttcgg gataatcttt tcggcggagc agtttcatac
      841 ccaaaacgtt ttggtagaaa tcgagggatt tttcgagatt gcccacgcgg agcatagtat
      901 ggagtaagcg cattttttgt gttcctttcg gtggtggtta aacttcgatt ttattcgggg
      961 taaacgtctg ccatttgttg caggcggggc agtgccagaa aaagacttgg gatttgaagt
     1021 ggcagttgcg gcaacggtac atcacgctgc gctgtagctg ccgtccgata accgaacgca
     1081 tcatgtcggc atcggctttc caagccggat tcatatcgct gagtttcaaa ccgagcaggc
     1141 ggtacacgcc gttaaggtcg ggcttgcggc ggacaagctc gacggcggtt tgcgcggctt
     1201 ctttctcgca cttaagcagc agggatttct cgtacacgac attgatcagg tcaagttcgg
     1261 gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt ccctgcgcgg
     1321 cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt tgctgctcga
     1381 tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttcg atgtcgccca
     1441 aaatcatgtt ggcgcgggtg cattttttgt tggcttcgag tgccttgccg acattgaaac
     1501 gcgcgacatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa taaaactggg
     1561 cgatttcaaa ctgataggtc tgatcgtcat ggctgagcag ccgggcggtt tcaaccgctt
     1621 tttcccaatc cctgtcctgt tggtagatat tgagcaggtg ctgtctggct tcacgcgcca
     1681 ttttaccgtc ttgcagcccc aaaaaaatct gttcggcacg atcgaccaac cccgcacttt
     1741 ggtagttttg cgccaattca aacaggacgc gcgcgcgctt ttcgccgacc gtatcgggag
     1801 aatcgagcat tgtccggtgt atgttgatgg ctttgtcgtt ttcgccacgc tggcggtaaa
     1861 gtttgccgag ggtgaggttc aaatcatacg attgcggccg gccgtcgacg acttccgcca
     1921 actcccttgc cgcgcgcccg ctgttgcggt cgaccaaagc gtccaagctt ttataaaatc
     1981 ccgaagggat gctttttgcc tgcttcaata cggttttcat atccacgcgg gcggcaaacc
     2041 agcccatcgc gaagaagacg ggcaaaagga taatcggcag caggataatc cacaattcgt
     2101 tgtccatatc ggctttctta aggctgtttg gtagattcgg gcgcattttg cgccggtggt
     2161 gcggtcagct ccttccccgt caaacgcgca tttttcttta cttcggcacg caacctgccg
     2221 ttctcgccac gtaacgacaa caaccgtccg aacaaggcaa acattccaaa aataataccg
     2281 actacaaatg cgccgaacaa tacgacaatc agcggcaaat cgaatttttg ccccggcagg
     2341 taggaaaagg taacggcatc cgtattaatg acggcaagca gcaggaagag cagcaggata
     2401 atgattttga tgacggtata gataagtttc atgtttgctc cgtaaataaa aacgccgccc
     2461 agacagcttc gcactctgaa ccaagattgc gctagtttaa acgatttgca cggtttcgga
     2521 taggatgcgg cagcgtgttc ggacgacata cggagtatgc gattgccgac aattttacca
     2581 aatacaccgt tcctttccat ttgaaaaata acggattgga caccgcatcg acagaaaaac
     2641 ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaatctt caaattcaaa
     2701 ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga cccgtacccg
     2761 ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc gaaggcaaat
     2821 ggcagccgac cgaatggcaa tcttcccaag acctctccct cgcaccgggc gcgcacgccc
     2881 tgcactacgg cagcgaatgt ttcgagggac tgaaagcctt ccgtcaggca gacggcaaaa
     2941 tcgtgctgtt ccgtccgact gccaatatcg cgcgtatgcg gcaaagtgcg gacattttgc
     3001 acctgccgcg ccccgaaacc gaagcttatc ttgacgcgct aatcaaattg gtcaaacgtg
     3061 ccgccgatga aattcccgat gcgcctgccg ccctgtacct gcgtccgacc ttaatcggta
     3121 ccgatcccgt tatcggcaag gccggttctc cttccgaaac cgccctgctg tatattttgg
     3181 cttcccccgt cggcgactat ttcaaagtcg gatcgcccgt caaaattttg gtggaaaccg
     3241 aacacatccg ctgcgccccg catatgggcc gcgtcaaatg cggcggcaac tacgcttccg
     3301 ccatgcactg ggtgctgaag gcgaaagccg aatatggcgc aaatcaagtc ctgttctgcc
     3361 cgaacggcga cgtgcaggaa accggcgcgt ccaactttat cctgattaac ggcgatgaaa
     3421 tcattaccaa accgctgacc gacgagtttt tgcacggcgt aacccgcgat tccgtactga
     3481 cggttgccaa agatttgggc tataccgtca gcgaacgcaa tttcacggtt gacgaactca
     3541 aagctgcggt ggaaaacggt gcggaagcca ttttgaccgg tacggcagcc gtcatctcgc
     3601 ccgttacttc cttcgtcatc ggcggcaaag aaatcgaagt gaaaagccaa gaacgcggct
     3661 atgccatccg taaggcgatt accgacatcc agtatggttt ggcggaagac aaatacggct
     3721 ggctggttga agtgtgctga tgctttaaat aaaaaatgcc gtctgaaacc cgtttggcgt
     3781 ttcagacggc attttcgcat ccgaaccgtt tccgctgcac ctgcagcaag tcggcacaaa
     3841 ggcaatcggt taaaacaagc gtccgcattt cccatcccgc ctgccgtaag tcgggcattt
     3901 ccctagaaat acgcttcaga cggcaaaacg ccgcccgaaa ccgatatgcg gcacggacgg
     3961 cgcacggatt tgaaaacggc ggattatccc tcggtgctca aggcattaat gctgtaaccg
     4021 ccgtcaacgt aagtgatttc gccggtaatg ccggacgaca ggtcggacag caggaaggcg
     4081 gcggtattgc cgacttcttc aatggtaacg ttgcggcgga gcgggttgtg ggcggcgacg
     4141 tgtcccaaga gtttgccgaa atcggcgatg ccggaggcgg caagcgtttt aatcgggccg
     4201 gcggaaatac cgttgcagcg gatgccctct ttacccagac aggcagcggt aaagcggatg
     4261 cctgcctcaa ggctggcttt tgccataccc atcacgttgt aattcggaat cgcgcgcacc
     4321 gcgcccaagt agctcagggc gacgatggcg gaatttctgc cgcgcatcat cggacgggcg
     4381 gcttttgcca acgcgggcag gctgtatgcg gaaatttcgt gtgcggtgtt gaacgcttcg
     4441 cggctgatgc tgtcgaggaa gtcgccgctc aaggcttctt tcggcgcaaa accgatggaa
     4501 tgcaccaaac cgtccaagcc gtcccaatgt ttgcccaagt cggcgaacac ttggttgatt
     4561 tcgtcgtcgc tggcgacatc gcagcggaat acaagttcgg aatccaattc cgccgccatt
     4621 ttgcggacgc gctcttccag tttgtccaca acgtaggtaa acgccagttc cgcgccttgt
     4681 tcgcggcagg ctttggcgat gccgtaagcg atggaacgct cggaaatcat gccggtaatc
     4741 agaatttttt tgccttgcag aaaacccatt ttgtatcctt caaatagtgt tcggttcgtt
     4801 ctaaaccgtt gattatagca aattgtccct gtttctgtgt tttcacgttg cagcgtgcaa
     4861 acggcaatgc cgtctgaagc ggatttcaga cggcattgga cgtttcaaat acggtttaag
     4921 gcatcagatg ccgcgcaaca attcgttgac gctggttttc gcacgggttt gcgcgtccac
     4981 gcgtttgacg atgacggcgc agtaaaggct gtggctgccg tctttggaag gcatactgcc
     5041 ggatacgaca accgaacctg ccggtacgcg gccttgatag atttcgccgg ttgtacggtc
     5101 aaagattttg gtggattgac cgatgaacac gcccatagaa atcacgctgc cttcttcgac
     5161 aatcacgccc tcaacgattt cagaacgcgc accgatgaag cagttgtctt caatgatggt
     5221 gggtgcggcc tgcaggggtt cgagtacacc accgatgccg acgcccccgc tcaagtgcac
     5281 gtttttaccg atttgcgcgc aagagccgac ggttgcccaa gtatcgacca tcgcgccttc
     5341 gtcgacgtat gcgccgatgt tgacataaga tggcatcagc acgacatttt tcgccacaaa
     5401 gctgccgcgt cgggcaaccg cacccggaac tgcgcggaag cctgcgtttt tgaactcgtc
     5461 ttcagaccag tcggcaaact tggtcggcac tttgtcgaag tatttgttca cgccgtcgtt
     5521 gaggacttcg ttgtcttgga tgcggaagga cagcaacacg gcttttttcg cccattcgtt
     5581 gactttccac tcacccacgc ccaaacgttc ggcaacgcgc agtttgccgg aatcgagttg
     5641 gcggatggtt tccaacacgg cttctttgac ttcgggagta acggtggtcg gggtgatgtc
     5701 cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa gacataatat ttccttatgt
     5761 gagatgtttc gtgggacgct tgccggtatt ttcaagcagt atgttcattt gaaatgaatg
     5821 cgctattgtg ccacgcaaac gcatattcgt cagcaatacg cgagcggttg ccaaacattg
     5881 gcgtttcgga atcgggcagg cggcaggtat tgccttttcc gcgcccgaat caatacagaa
     5941 aggcggcagt acttttatgc cgcgccgccg cctttcagac ggcattcgcg gtaaaacgcc
     6001 atcagccctt cggtcgaggc atcgtgtacg gacgtgccgc cttccagttc gccgatgatg
     6061 gtttttgcca actgtttgcc gtattccacc ccccactgat cgaaggggtt gacgttccat
     6121 atcgcgcctt ggacgaaggt tttgtgttcg taagccgcca tcagcatacc caaattgtag
     6181 ggcgtgaggc ggtcaatcaa aatgctgttg ctggggcggt tgccggggaa ctctttgtgc
     6241 ggcgcgaggc gttcgcgttc cgcttcgggc aaatctgcca gttcggcgcg tgcttcgtcc
     6301 aaggttttgc ccttcatcaa ggcttccgct tgggcaaagg cgttggcaac ggtaaaacgg
     6361 ctgcgtccgt cctctctgcc ctgcgccgtc atcgggacga taaaatcgca gggaatcagg
     6421 cgcgtgcctt ggtggagcag ttggaaatag gcgtgctggc agttgacccc ttcaccaccg
     6481 aacacgatgc cgcccgtttt gcacacggcg ggactgccgt ctgaagcgcg gcttttgccc
     6541 aaactctcca tatcgagctg gttcagccac gccggcagca ggcgcaggtt gtggctgtac
     6601 ggaacggcgg tctgcccgtc cgcgtgctgg aaattgttgt accacacggc aatcagtgcc
     6661 attaaaacgg ggatattatg acgcgtcggc gtactgaaaa aatgcctgtc catcgcgtgc
     6721 gcccccgcca acaactcgcg gaaacgcgcc ccgccgaccg caaccatcac gggcaaaccg
     6781 acgggcgacc agacggaata gcgtccgccc acccagtcgt acatcgcaaa cacgcgttcc
     6841 gccgcgatac caaaagccgc agctgccgca gtgtcggcag acaccgcgca aaaatggcac
     6901 gccgtttcgg attccgagaa ccctgcaccg cgataccacg ccttgactgc ctgtgcattg
     6961 agcagggttt ccggtgtttt gaaggacttg ctggcaacgc aaaacactgt cgtttcgggg
     7021 ttcagacggc ataaaaccgc atccaggcag gcaggatcgg cgttggcggc aaaatggacg
     7081 gtgatatgcc gtctgaacgg ctcaagtgcc tgcacgcaca ttgccggccc gaggtcggat
     7141 ccgcctatgc cgatgtggac aaaatccgta atccgttttc cggttatccc ctgatacgaa
     7201 ccgtcgtcca aactgtgtgc aaacttcaac gcacgattta actcgcggcg gatttcgggc
     7261 aacacgtccc tgccgtccac ataaacggca tccgcaccgt cgggcaggcg caaagccgta
     7321 tgcagcgcgg cacgcccctc gctgccgttg actttcgcac ccgtccgcaa agcacgcatt
     7381 ttcccttcca aatccgccgc gtcggcaaga ttgcagagca gttgcagcgt atcttcgccc
     7441 aaacggtttt tgctgtaatc gaacaacatc ccgtccaaac gctcgtgcat acgctcaaac
     7501 cggtccggtt cgcaggcaaa gcggtcgcgc aaaaggacat gacgcgtatc ctgataatgg
     7561 cgttcgagcg cataccatgc acgggtaaaa gcattcatct gttttccttg atttttcaga
     7621 accggattaa aatgtagcag aatgtagttt aacaaacggc agcggctttg gcgaatctcc
     7681 ggaacaccgc ccgccgcgtc caatagcacc ttatttccat atggcggaaa tgccctccgg
     7741 ccggcatttg acccgcaaca atatcctgca agatttattg tgtacgcata aatgccggac
     7801 agccgcctaa ataaggtata atcgcatccg attctgtccc gtttgccgcc cgcaggcggg
     7861 atgtcggcgg ttttcatctt accgggaaca cgccggtact gttttttaac gatgtttctt
     7921 acattttatt ccaattactt tacggggcta gaatatggct aaaaacggag gattttcttt
     7981 gttcgcaaag aaagaaaaac gctttatctt tgaaggcagg cattccgcct ccgacaaact
     8041 ggtcaacggc gaagtatccg cgtttaccga agaagaggcg cgcaaaaaac tggcaaaacg
     8101 cggcatccgc ccgttgcaga ttacccgtgt gaaaacaagc tccaagcgca aaatcacaca
     8161 agaagacatc accgttttca cccgccagct gtccacgatg attaaagcgg gcctgccgct
     8221 gatgcaggca tttgaaatcg tggcgcgcgg acacggcaac ccgtctatga cggaaatgct
     8281 gatggaaatc cgaggcgaag tggaacaggg cagctcgttg agccgcgcat tctcaaacca
     8341 cccaaaatat ttcgaccgct tctactgcaa tctggttgcg gcgggcgaaa cgggcggcgt
     8401 attggaaagc ctgctggaca aattggcaat ttacaaagaa aaaacccagg ccatccgcaa
     8461 aaaggtaaaa accgcactga cctatccggt atccgtcatc gccgtcgcca tcggtttggt
     8521 attcgtgatg atgattttcg tactgcccgc ctttaaagaa gtttacgcca atatgggcgc
     8581 ggagcttccc gcactgaccc aaacagtgat ggatatgtcc gactttttcg tctcatacgg
     8641 ctggatggtg ctgatcgcac tgggctttgc catatacggc ttccttaaat tgaaggcgcg
     8701 ttcgattaaa atccaacggc gtatggatgc catactgctg cgtatgccga ttttcggaga
     8761 cattgtccgc aaaggaacga ttgcccgctg gggcaggacg acggcgacgc tgattgcggc
     8821 aggcgtgcct ttggtcgatg tattggactc cactgccggc gcggcgggca atttaatcta
     8881 tgaagaagcc acccgggaaa tccgtacgcg cgtcatccaa ggtttgtcta tgacttcggg
     8941 gatgcgtgcg acggaactgt tccccaatat gatgttgcag atgtcttcca tcggcgagga
     9001 atcgggttct ttggacgata tgctcaacaa agccgccgaa ttttacgaag acgaggtgga
     9061 caatgcggtc ggcaggctgt ccgctatgat ggagccgatc attatcgtga ttttaggctt
     9121 ggtcatcggc acgcttctgg tcgccatgta tctgccgctg tttaatttgg gcaacgtggt
     9181 cgcctgattt gccgcacaga tccggcgcgg attcgctctg cgccggtttg tttttgtttt
     9241 gaatacattg agaacaaaat atgtctgatt tgtctgtatt gtcgccgttt gccgtgcctt
     9301 tggcagcggt gttcgggctg ctggtcggaa gtttcttaaa tgtcgtcatt taccgcgtgc
     9361 cggtcatgat ggaacgcggc tggacggtat ttgccaaaga atatttaaac ctgccgctga
     9421 ccgaagagga aagccgtacc ttcaacctga tgaaaccgga ttcctgctgt cccaaatgcc
     9481 gcgtgccgat acgcgcgtgg cagaacatcc cgattgtcag ctacctgctc ctgcgcggca
     9541 aatgcgcttc ctgccaaacc aaaatcagca tacgttatcc cttaatcgag ctgctgaccg
     9601 gcgtattgtt cgggctggtc gcctggcaat acggctggtc ttggattacg ctgggcggat
     9661 tggtactgac cgcgtttctg atttccctga cctttatcga tgcggacacc caatacctgc
     9721 ccgactcgat gacactgccc ttaatttggc tgggtctgat atttaatttg gacggcggct
     9781 tcgtgccttt gcagtctgcc gttttaggtg cggtcgccgg ctatggttca ttatggctct
     9841 tatgtgcagt gtataaactg ctcacaggaa aaaccggtat gggcaacgga gatttcaaac
     9901 tgattgccgc attgggcgcg tggctcggca tatccgcatt gcccgtactg atttttgttt
     9961 cctcgctgat cggtttggtc gcggcaatcg ttatgcgcgt cgccaagggg cagcattttg
    10021 ccttcggccc cgcactgaca gtttcgggct ggataatttt tacggcaaac gattccgtat
    10081 ggcgggcggt caactggtgg ctgacccatc cggtgctgta agatgacggt atgggtcgga
    10141 ctgaccggcg gaatcggcag cggcaaatcg gcagccgcgc aatgttttgc cgatttgggc
    10201 gtgccgcgca tcgatgcaga cgcggcggcg cactcgctga cggcttcaga cggcatcgcc
    10261 ctgccggaaa tcaggcggct gttcggcgac accgtttttg acacacaggg tttgttgcgg
    10321 cgcgacatat tgcgtaaaga agtctttgcc tccccatcgc gaaaagcctt gctcgaatcc
    10381 gtgatgttgc cgctgatttt ctcagaaatc aaaaaacagc aagaaacctt tactgatgca
    10441 gcttacggca ttgtcgaaat tccgctgctg acggaaaagc gtcaatttat cagcctgata
    10501 cggcgtgtcc tgaccataag tgcccctgtg gaaaaacgta tcggcagggt gatggcccgc
    10561 agcgggctga cgcgcggcga ggtggcggcc gtcatcagcc atcaggcatc cgaatccgaa
    10621 cgcctgctgc ttgcagacga tgtgctgctc aatgacggca gcctcaaaag cctgcgtgag
    10681 aaaacaatgc gcctgcacgc gttttattca gggattttcg cctcaaaacc aacacaagga
    10741 aaacacaatg actgaatcgc ggcaaacacg ccttcaagtc aaatgtccga cctgtcaaac
    10801 agcagtagta tggaaacccg aaaacgcatt ccgccccttc tgttcgcaac gctgcaaact
    10861 gatcgacttg ggcggatggg cagacgggaa atatacggtt tccggccaaa cggaaagttt
    10921 gccggaaata tccgaacccg acatggcata ccgctgaccg ccccgccttc ccggcaaaca
    10981 ccctgaaagt caaatgccgt ctgaaacaaa cacgcttcag acggcatttt cattctcaaa
    11041 cctaatcgtt ggtatttgcc gttacctctt ccaatgaagt aatgccctgc ataactttca
    11101 aaataccggc ccggcgcaaa tccaccatac cctccttata ggcaacgtcc aaaatatcca
    11161 cttccgtacc gttgttcata atcacacgct gcatttcttc gctgatgggc ataacctcat
    11221 acacgcccgc acgcccctta taaccctgcc cccggcaacg gtcgcaaccg acggcgcggt
    11281 aaagtttcca atcttttgca aggtcctcat cggtgaagcc gacttccttc aaagcagagg
    11341 cagacgggcg ttccacttcc tgtttgcagc tcgaacacag cctgcgtaaa agacgctgcg
    11401 ccataatcag gctgaccgaa ctggcaatat taaacggcgc gacacccata ttcagcatac
    11461 gcgacaacgt cgccggcgca ttattggtgt gcagggtgga aaacaccata tgccctgttt
    11521 gtgccgcctt aatcgcaata tcggcagttt ccaaatcacg aatctcaccg accataatga
    11581 tgtccgggtc ctgacgcagg aaagacttca aagcagcggc aaaagtcagg ccctgcttat
    11641 cattgacgtt aacctgattg atgcccggca ggttaatctc ggcagggtct tccgccgttg
    11701 caatatttac cgactccgta ttcaaaatat tcaaacaggt atagagcgac accgtcttac
    11761 ccgaacccgt cggaccggtt accagcacca tcccgtaggg acggtgaatc gcttccaaca
    11821 acaatttttt ctgaaacggc tcaaaaccga gctggtcgat gttcaaagac gcggcatcgg
    11881 aattcaaaat ccgcatcacg accttttcgc caaacagcgt cggcaatgtg ctgacacgga
    11941 aatcgacagg cttgccgccc ttttgaaagg tcagctgcat cctgccgtcc tgcggtatcc
    12001 gtttttcgga aatgtccaaa cgcgacatta ccttaatccg tgaagcaagc tgccccctta
    12061 ccgcaatggg cggctgaacc acctcgcgga gctgcccgtc cacacggaaa cggatacggg
    12121 cattgtgttc gtaaaactcg aaatggatgt ccgatgcccc gctgcgcaag gcatccgaca
    12181 aagtcttatg gataaacctc ggaacagggc cgtcttctgc ctcctcgttg tcgatataca
    12241 gggtgtggct ttcctcttcc tcctgcccct ccccaagctc ctgaagcagc gatgtcgaac
    12301 gcgaacccac ccaatcgagc aaacccgcca actggtcatc ctcgacaatg accaactcaa
    12361 cctcaatccc tgcggcagaa acggttttct gaatttgcgg catctgtgtc ggatcggaaa
    12421 ccgcaaaaaa tactttgtcg ccccgacgga aaaccggcac acagtggaac tccaccatct
    12481 gctcctccgt caacaccccc atcagcaccc tgtggcgcgg ataatgacgc aaatcaagaa
    12541 tcgaataact gaacaccctc gcaatcaatg ccgcaagcga cttgggcgaa atgacaccgt
    12601 ctgaaaacag catcggcaac acttccttac ccgcctgcga ctcattgtag taatgctcgg
    12661 cctgctcaac agtaaccacc tggttttgaa ccagaatcct cagcaaacct acgctcatac
    12721 gaccttatcc ccaaatttat tcattgttat acctgtacag ct
//
LOCUS       DAWISC010000043        12248 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWISC010000043 DAWISC010000000
VERSION     DAWISC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.043"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            243..980
                     /locus_tag="WIW87_000869"
     CDS             243..980
                     /locus_tag="WIW87_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174050.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWLSEKIKARVKECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            982..1668
                     /locus_tag="WIW87_000870"
     CDS             982..1668
                     /locus_tag="WIW87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5174051.1"
                     /translation="MADLTFQQAVSTIVGMKDEIFRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKLVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIIFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1827..2690
                     /locus_tag="WIW87_000871"
     CDS             1827..2690
                     /locus_tag="WIW87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174052.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2752..3321)
                     /locus_tag="WIW87_000872"
     CDS             complement(2752..3321)
                     /locus_tag="WIW87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ5174053.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3479..4339
                     /locus_tag="WIW87_000873"
     CDS             3479..4339
                     /locus_tag="WIW87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ5174054.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEVKTANHKKGRIVLYFDTPETFGHL
                     LEQLGIDYRP"
     gene            complement(4535..4972)
                     /locus_tag="WIW87_000874"
     CDS             complement(4535..4972)
                     /locus_tag="WIW87_000874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174055.1"
                     /translation="MFLRDLFALRGNNKKSTRNCSSLSVGLSAVWGICSINKRSSENI
                     FRRPFPNKGLATACRFKQSIALTFAFVRSASQTNCIGSGNADCADYTGNIALAHALTH
                     SCGKVDAYAFIVAVADFYGIWADAQGKRGTFLKFLVGNGYIID"
     gene            5181..5534
                     /locus_tag="WIW87_000875"
     CDS             5181..5534
                     /locus_tag="WIW87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ5174056.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5524..6390
                     /gene="atpB"
                     /locus_tag="WIW87_000876"
     CDS             5524..6390
                     /gene="atpB"
                     /locus_tag="WIW87_000876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ5174057.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6447..6683
                     /gene="atpE"
                     /locus_tag="WIW87_000877"
     CDS             6447..6683
                     /gene="atpE"
                     /locus_tag="WIW87_000877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ5174058.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6754..7224
                     /locus_tag="WIW87_000878"
     CDS             6754..7224
                     /locus_tag="WIW87_000878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ5174059.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7229..7762
                     /locus_tag="WIW87_000879"
     CDS             7229..7762
                     /locus_tag="WIW87_000879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ5174060.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELVQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7773..9320
                     /locus_tag="WIW87_000880"
     CDS             7773..9320
                     /locus_tag="WIW87_000880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ5174061.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTCGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9345..10220
                     /gene="atpG"
                     /locus_tag="WIW87_000881"
     CDS             9345..10220
                     /gene="atpG"
                     /locus_tag="WIW87_000881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ5174062.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCFGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10258..11655
                     /gene="atpD"
                     /locus_tag="WIW87_000882"
     CDS             10258..11655
                     /gene="atpD"
                     /locus_tag="WIW87_000882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ5174063.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11666..12088
                     /locus_tag="WIW87_000883"
     CDS             11666..12088
                     /locus_tag="WIW87_000883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ5174064.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKVLAAAIAQLKTLDYIRSHKK"
BASE COUNT         3008 a         3140 c         3162 g         2938 t
ORIGIN      
        1 tcaattattt gccgttttag tgcgaaccgc tgcctttgcc cgtttcagac ggcattgtcc
       61 gaaatggttg cccgcttcct gccttattga caaaaaaacg ctttcccgat aatatcctac
      121 gaaaattaac ctgccgattt gacacagctt gcgggcataa cagctaaagc gttccgacaa
      181 tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg gctttgttgt atgggaaaga
      241 caatgattat tttggacaag gtttccaagc attaccaaac gcgcgacaag acccgttttg
      301 ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga aatcttcggg ctgatgggtt
      361 attcgggtgc aggcaaatcc accctgttgc gcctgattaa cctgttggaa cgccccgaca
      421 gcggcaaggt caacgtctgc ggacaagagc taaccgcgct cgatgccgcc gcattgcgtc
      481 aggctcggca gaatatcggc atggtgtttc agcagttcaa cctgttgaac aaccgcaccg
      541 tcgccggcaa cgtcgccttc ccattggaaa tcgccggatg gctgtctgaa aaaatcaaag
      601 cgcgcgttaa agaatgcctt gaaatcgtcg gactgaccga acgcgccggc cactatcccg
      661 cccagctttc cggcgggcag aaacaacgtg tcggcatcgc ccgcgcactc gcgcccaaac
      721 cccaagtcat cctcgcagac gaacccactt ccgccctcga ccccgccacc acgcgcagcg
      781 tcttggaatg tttggaagac atcaacaaac gcttcaacgt aaccatcgtc atcgtaaccc
      841 acgaaatgag cgtcatccgc cgcctgtgcg accgcgccgc cctcttggat aaaggcaaag
      901 tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc ccaatccgac atcgggcgcg
      961 aactgattcg ggaggactga tatggcagac ttaacattcc aacaagccgt ttccaccatc
     1021 gtcggcatga aagacgaaat cttccgcgcc ttgggcgaaa ccttcgtgat ggtcggcttg
     1081 tccaccacat tcgccgtcat cttcggcacg ctgctgggcg tgctgctctt cgtaacctcc
     1141 agccgccaac tgcattacaa caagctggtg aacttcctgc tcgacaacct cgtcaacctc
     1201 atgcgcgcct tccccttcgt catcctgatg attgcgatga tacccgccac acgcgccatc
     1261 gtcggcagca ccatcggtcc ggttgccgcc tcgctggtgt tgagcgtgtc gggattgttt
     1321 tattttgccc gactggtgga acaaaacctg cgcgaagtcc ccaaaggcgt aattgaagcc
     1381 gccgccgcga tgggtgcgcc gccgattgcc atcgtctgca aagtcctctt gaacgaagcg
     1441 cgcgcgggca tggtttccag cattaccgtg cttgccatcg ggcttttgtc atacagcgcg
     1501 gcggcaggga tgataggcgg cggcggcttg ggcgacctcg ccatccgcta cggctactac
     1561 cgctaccaaa ccgaagtcat catcttcatc gtcgccctcc tcgtgctgct ggtcatcctg
     1621 attcaaagca ccggcaacgc gttggcgcgg aaactcgaca aacgttgaac ccgaatgccg
     1681 tctgaacgcc aaaaccccca ccgctatccg aaaaatgcta taaaatcccc ctgttcgcgg
     1741 caaatgccgt ctgaacgccg aatccggacg gcaggactcc ctgcccgtca tttttgtttg
     1801 aaactgccac aacatcagga gaaaatatga aaaccttctt caaaaccctt tccgccgccg
     1861 cactcgcgct catcctcgcc gcctgcggcg gtcaaaaaga cagcgcgccc gccgcatccg
     1921 cttctgccgc cgccgacaac ggcgcggcga aaaaagaaat cgtcttcggc acgaccgtcg
     1981 gcgacttcgg cgatatggtc aaagaacaaa tccaagccga gctggagaaa aaaggctaca
     2041 ccgtcaaact ggtcgagttt accgactatg tacgcccgaa tctggcattg gctgagggcg
     2101 agttggacat caacgtcttc caacacaaac cctatcttga cgacttcaaa aaagaacaca
     2161 atctggacat caccgaagtc ttccaagtgc cgaccgcgcc tttgggactg tacccgggca
     2221 agctgaaatc gctggaagaa gtcaaagacg gcagcaccgt atccgcgccc aacgacccgt
     2281 ccaacttcgc ccgcgtcttg gtgatgctcg acgaactggg ttggatcaaa ctcaaagacg
     2341 gcatcaatcc gttgaccgca tccaaagcgg acatcgccga gaacctgaaa aacatcaaaa
     2401 tcgtcgagct tgaagccgcg caactgccgc gtagccgcgc cgacgtggat tttgccgtcg
     2461 tcaacggcaa ctacgccata agcagcggca tgaagctgac cgaagccctg ttccaagaac
     2521 cgagctttgc ctatgtcaac tggtctgccg tcaaaaccgc cgacaaagac agccaatggc
     2581 ttaaagacgt aaccgaggcc tataactccg acgcgttcaa agcctacgcg cacaaacgct
     2641 tcgagggcta caaatcccct gccgcatgga atgaaggcgc agccaaataa ggcagtcgta
     2701 taaaatgatg ccgtctgaac tgtatccgtg ttcagacggc atttttgtcc tttaatccgc
     2761 cattccctgc cattccgccg aatccggcgt atcgattccg aacagcgaca aagcgtgtgc
     2821 aacactgtgc gccactatgt cgtccgccgt ctgcggtttg cggtacatcg caggaacagg
     2881 gggaaacacc acgccgccca tttccgttac ccgcttcata ttgtccaaat gggcaaggtt
     2941 cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt tccttcaaaa ccacatccgc
     3001 cgcacgcgtc agcagattgt cgccgaagcc gtgcgcgaca gaggcaagcg tccgcatcga
     3061 acagggggcg accagcatcc catccgtttt aaacgtaccg ctggcaatgc acgccccgat
     3121 attgccgatc ggatgcacga agtccgccaa ggcatatacc tcgtctctcg cataagccgt
     3181 ttccgaagcg cgcgccatct ccgcaccttt cgataccaca aggtgcgttt cgacatcttg
     3241 cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa ccgctcgccc cgctgatgcc
     3301 gattatcaaa cgccgtacca tcatccgcct ttcccataaa accgcctgca acggcaaacc
     3361 ggctattata gtgaaaaaac agaaatccga taaacgcgga tacaaattgt cggcaacacc
     3421 caatatccga taaaataccc gatttaacat cctatctgaa taggcacggg agggcggtat
     3481 ggcaaaagta aaaggcggat tggggcgcgg cttggattcg ctgctcgcca acggcgcgga
     3541 caacagcagc ggcgaccgat tgaccacggt tgcggttaaa gatatccggc ccggccgcta
     3601 tcaggcgcgt gttcaaatcg atgacgaagc cttgcaggaa ctggcagatt cgattaaggc
     3661 gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga ctgtcccgat acgaactgat
     3721 tgcaggcgaa cgccgttggc gcgccgcaca gattgccggc ctgaccgaaa tccccgccgt
     3781 tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt ttgatcgaaa acctccagcg
     3841 cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa cgccttgccg acgagttcgg
     3901 gctgacccac gaaaccatcg cccaagccgt cggtaaaagc cgaagcgcga tttccaacag
     3961 cctgcgcctt ttaagcctgc ccgaacccgt gcaggaaatg ctttaccaac gccgcctcga
     4021 aatggggcac gcccgcgcat tgctgaccct gcccgtcgtc gaacagctcg aattggcgca
     4081 aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa cgccgcagcc aggccgccct
     4141 tcaaaacaaa cgtcccgagc ccaaaaagac tgccgccgcc gacatcggcc gcctgaatga
     4201 tttgctgact gaaaaactgg gtgtcaacgc tgaagtcaaa accgccaacc acaaaaaagg
     4261 caggattgtc ctgtatttcg atacgcctga aacgttcggc cacctgctgg agcagttggg
     4321 catagattac cggccttaat tttgcgggat ataccgtctg aaatatagag aatagctttc
     4381 cagattttaa gtgggaaata taattctatt gacatttttt ctgcttcacg taagaatcgt
     4441 tttcctgttt tcatttttaa ttttcgagga aattatgaac acacgcatca tcgtttcggc
     4501 tgcgttcgtt gcgttggcat tagcaggttg cggctcaatc aataatgtaa ccgtttccga
     4561 ctagaaactt caggaacgtg ccgcgtttgc cttgggcgtc agcccaaatg ccgtaaaaat
     4621 cagcaaccgc aacaatgaag gcatacgcat caactttacc gcaactgtgg gtaagcgcgt
     4681 gagccaatgc tatgttacca gtgtaatcag cacaatcggc gttaccactt ccgatgcaat
     4741 ttgtttggga ggcggaacgc acaaaggcaa aagtcaatgc aatgctttgc ttaaagcggc
     4801 aggcagttgc taatccttta ttcggaaaag gtcgtctgaa aatattttca gacgaccttt
     4861 tatttattga gcaaattccc caaactgccg acaatccgac cgataaactg ctacaatttc
     4921 gcgttgattt tttattattt ccacgtaaag caaacaaatc acgtaagaac attaaaaatg
     4981 taaagaaatt gcggacaaac tccttaatat cctgtaatta tatgtaatgt gtgttagaat
     5041 gacacctgtc gccgttattc aggcggcgag cgcggcggat tgggatacgt tgtttcacaa
     5101 agattaactg tcgcttgacg taataaggct tctaaattat attagccgcg ccccaaatcc
     5161 gaggcggaaa cattcccttt atgaagcaga ttatcatcct acaatccgcc gtattgtcca
     5221 tatgcgccgc agttgccttt gctgtatggg gttttgccgg attcctttcg gcggtcggtg
     5281 gcggtttgtc ctacctgctt cccacgtttg ttgcagtttt acttttaaaa cttttcaggg
     5341 gaaacccctt cctgcaaagc aggatgttcg tcttcggaga gattttaaaa gtagtgctgt
     5401 cgctgttgtc catgcttgcc gtatttgcaa tatggcatca atcgctggtg ttcgccccgt
     5461 ttctgatggg gctgctcggt gtcagccatt tggttttttt agtattgttg agagtgaaag
     5521 attatggcag gtgaaaccat taccgctgcc gactacatca agcaccactt gcagagcttg
     5581 accagtttgt cggatgttac tcagggtcag ggactgaaaa acattgctga tttttcgttt
     5641 attaaccttg atgccgtctt ttttgccgtc ctgttgggcg taatcggcag ctttctgttg
     5701 tggcgcggtg caaaaaaagc gacggcaggc gttcccggac gtttccaggc cgcagtggaa
     5761 atcttgtttg agtttgtgga cgatatgtgt aagagcatca ttcacaacga aaagtctaga
     5821 aaagccgtcg cgccgttggg tctgacgctg tttgtctgga tttttctgat gaacgcgatg
     5881 gatatgctgc cggtcgattt gctgccgatg gtatggcagg gcattaccgg caaccatcac
     5941 gccctgctgc gcgtcgtacc gactgccgat ttgaacacca ctttggcact cgctgtcggc
     6001 gtgttgctga tttgtatcta ttacaacatc aaaatcaaag gattgggtgg ctggtttcac
     6061 gagttgttca gcgcgccgtt cggtgcaaaa ctcgcacctg cgaactttct gttgaatttg
     6121 gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt tgttcggcaa tatgtatgcg
     6181 ggcgaattgg tattcttgtt gatagccttg cttggcggtg cttgggcggc ttccggcagc
     6241 gtcgaagtca tggatccgat tctgtttgta ttccacatta ttgccggttt ggcatgggcg
     6301 attttccata ttttggtgat taccctgcag gcgtttattt tcatggcgtt ggcgttcgtc
     6361 tatatcggac aggcacatga tgcgcactga ttttcctaag tagtttgttt ttgtagttta
     6421 acctttgttt ctttaaggag tttaaaatgg gtttgattgc tatcgcatgt ggtttgatcg
     6481 ttgcattggg tgcattgggt gcctctatcg gtatcgcaat ggtcggttct aaatatttgg
     6541 agtcttctgc ccgtcagccc gaactgatcg gtccgctgca aaccaaactg ttcctgattg
     6601 ccggtttgat tgatgccgca ttcttgattg gtgtcgccat tgcactgctg ttcgccttcg
     6661 ttaacccgtt tgcaggtgca taatcaggac ggattcgtcc gttccgtaca ggcgcagcct
     6721 gtttgattaa ccccagtacg aaggttaagt aacgtgaata tcaatgcaac attattcgct
     6781 caaatcatcg tctttttcgg tttggtatgg tttaccatga aattcgtgtg gccgccgatt
     6841 gcaaaagctt tggatgagcg tgccgcaaaa gtcgccgaag gcttggctgc cgccgagcgt
     6901 ggtaaaagcg attttgagca ggctgaaaaa aaggttgcag aactcttggc agaagggcgt
     6961 aatcaggttt ccgaaatggt tgccaacgcc gaaaaacgtg ccgccaaaat tgtcgaagaa
     7021 gccaaagaac aggcttcttc cgaggcggcg cgcattgcag ctcaggcaaa ggccgatgtg
     7081 gagcaggaat tgttccgcgc acgcgaatcc ctgcgcgagc aggttgccgt gttggctgtc
     7141 aaaggtgccg aatctatttt gcgcagcgaa gtcgatgctt ccaaacacgc aaaactgctc
     7201 gataccctga aacaggagct gtaatcttat ggcagagttc gcaacgattg ccagacctta
     7261 tgcaaaggca ttgttcggtc tggctcagga aaaaaaccaa attgagtctt ggttgggcgg
     7321 actggaaaaa cttgcggcgg ttgttcagga agggaaggtg gcttcattga ttgaccgtcc
     7381 tgaaacgaat gcttcagaaa aagcagatat cctcatcgat ttggtcggtt tgaaagacaa
     7441 ggagttgaaa aactttgtta tcgtcttggc cgggcagaaa cgtttgtcga tattgccgga
     7501 agtatatgct caatatcaag acttgacctt atcattcaac catatcaaat ctgccgtcat
     7561 ttacagtgcc tatccgttga ccgacaaaca ggtcggcgag ttggtgcaaa tgctgaataa
     7621 gcgtttcgac agcgagctga aaatctctgt cgaaatcgaa ccggagctga ttggcggcat
     7681 aaaagttgaa gtgggtgatc aggttttgga tttgtctgta caaggcaaat tgagtgcctt
     7741 gtacacgact atgacgaatt aggagagttt tcatgcagct taatcctgct gaaattagcg
     7801 atttgattaa agccaagatc gaaaatctgt ctgtaaatgc cgaagtgcgt acctgtggta
     7861 cggtaatttc ggtaactgac ggtatcgttc gcatccatgg tttgtcagat gcaatgcaag
     7921 gtgagatgct cgaattcccg ggtaacactt tcggcttggc gatgaacctg gagcgcgact
     7981 ccgtcggcgc cgtagtgttg ggcgagtacg aacacattaa agaaggcgac acggttacct
     8041 gtaccggtcg tatcttggaa gtgccggtcg gacgcgaact ggtcggacgc gtcgttgatg
     8101 cattgggtcg gcctatcgac ggcaaaggcc cgattaatac aactttgacc gcccctatcg
     8161 aaaaaatcgc accgggcgtg attgcgcgca aatcggttga ccagccgatg caaaccggtc
     8221 tgaaggcgat tgactctatg gttcctgtcg gtcgcggtca gcgtgagttg attattggcg
     8281 accgtcagac aggtaaaacg gccgtagcat tggatgccat cgtcaaccaa aaaggtacgg
     8341 gtgttatctg tatctatgtc gctatcggtc aaaaagcatc ttctattgcc aacgtggtcc
     8401 gcaaattgga agagcatggc gcgatggagc ataccatcgt ggttgctgca actgcatctg
     8461 aagcggcggc attgcaatat atcgcacctt actccggttg tacgatgggt gaattcttcc
     8521 gcgatcgtgg cgaagatgcc ttgattgttt atgacgattt gtccaaacag gctgtggctt
     8581 accgtcaaat ttccctgctt ttgcgccgtc cgcccggccg cgaagcctat cccggcgatg
     8641 tgttctacct gcactcccgt ctgttggaac gtgcggcacg tgtcaatgaa cacgaagtgg
     8701 agaaattgac caacggcgaa gtaaaaggca aaaccggttc tctgaccgcg ttgccgatta
     8761 tcgaaaccca agcgggcgac gtatctgctt tcgttccgac taacgtcatt tcgattaccg
     8821 acggtcagat tttcttggaa accgacctct tcaacgccgg tatccgtcct gcaatcaatg
     8881 ccggtatttc cgtatcccgc gtaggcggtg ctgcacaaac caaagtgatt aaaaagctgg
     8941 gtggcggtat ccgtttggcg ttggcacaat atcgtgaatt ggcggcgttc tcgcaatttg
     9001 catccgattt ggatgaagct acgcgcaaac aattggagca tggcgaagtc gtaaccgaac
     9061 tgatgaaaca gaaacagttc agtacgttga atacggctga aatggctttg accctttggg
     9121 caatcaacaa cggttcgtat tctgatgttc cggttgccaa agctctggct tttgaatctg
     9181 aatttttgag ctttgtccgt acccaacatc cggaagtttt ggaagccgtc aatgcttcag
     9241 gtgcaatgtc cgacgagagc gagaaaacgc ttgaagcagc catgaaatcc ttcaaatctt
     9301 cttacgccta tcaggcataa ggcttgaagt gaaaggagtc tgaaatggca gtaggaaaag
     9361 agattctcac caaaatccgc agtgttcaga atacccaaaa gatcactaaa gcgatgcaga
     9421 tggtgtcaac ctctaaaatg cggaagactc aggaacggat gcgtttggcg cgtccgtatg
     9481 ccgaaaaagt gcgtatggtg atgagccatc ttgcgcaaac caataccgat catggtattc
     9541 cgttactgga atctcatcgg gaaatcagac gtgtcggttt tattttgatt acgtctgata
     9601 agggtttgtg tggcggtttg aacgccaacg tgctgaaaaa gtttttggca caagttcaag
     9661 agtatcggaa tcaaggtatt gaagaggaaa tcgtatgctt tggcagtaaa ggtctgatgg
     9721 cgtgtcagag cattggtctg aatgtggttg ccagtgccgt aaatttgggc gataccccaa
     9781 aaatggaaat gctgctcgga cctttgacag aactcttcca acggtatgag aaacatgaaa
     9841 ttgacagaat ccatctggtg tattcgggtt ttgtcaatac catgcgtcaa gaaccgagaa
     9901 tggaagtatt gctgcctatc ggtgagaacg tgattggcga ttcagctccc aaatcaccgt
     9961 tcagctggga ataccgctac gaaccgactg cacttgcagt gttggaatat ctggttcgcc
    10021 gctatttaga gtctgtggtt tatcaggcgt tgagcgacaa tatggcatcc gaacaggcag
    10081 cccgcatggt tgccatgaaa gctgcaacag acaatgcagg caatgccatc aaagagttgc
    10141 gtttggtata taacaaatcg cgtcaagctg cgattaccac ggaattgtca gaaattgtag
    10201 caggtgcggc ggctgtctga tgccgtctga atcctgtacg gaaattagga tacgataatg
    10261 agccaaggca aaatcgtaca aattatcggt gcggttgttg acgtggaatt tccacgcgac
    10321 atgattccgc gcgtttacga cgctttgaaa ttagacgaaa acggtctgac tttggaagtc
    10381 caacagcttt tgggcgacgg cgtagtccgt gccattgcga tgggcagctc ggacggtttg
    10441 aaacgcggca tgactgtgag caataccggt gcgcccatta ctgtgccggt aggtaaaggt
    10501 acgttgggac gcattgtcga tgtattggga acgcctgttg acgaagcagg tccgatcgat
    10561 accgacaaga gccgtgccat ccaccaagct gcccctaagt ttgacgaact gtcttccaca
    10621 accgaattgc tcgaaacggg cattaaagtg attgacttgc tgtgtccgtt tgccaaaggc
    10681 ggtaaagtag gtctgttcgg cggtgccggt gtgggtaaaa ccgtgaacat gatggaattg
    10741 atcaacaaca tcgccaaagc gcacagcggc ttgtccgtgt tcgcaggcgt gggtgagcgt
    10801 acccgcgaag gtaacgactt ctaccacgag atgaaagatt ccaacgtatt ggataaagtg
    10861 gcaatggttt acggtcagat gaacgaacct ccgggcaacc gtttgcgcgt cgcattgacc
    10921 ggtttgacca tggcggaata cttccgtgac gaaaaagacg aaaacggcaa aggccgcgac
    10981 gtattgttct tcgtggacaa catctaccgt tacaccctgg ctggtaccga agtatccgca
    11041 ttgttgggtc gtatgccgtc tgcagtgggc taccaaccga cattggcaga agaaatgggt
    11101 cgtttgcagg agcgtattac ctctacccaa accggttcta ttacttccat ccaagccgta
    11161 tatgtacctg cggacgactt gaccgacccg tctccggcaa caacgttcgc ccacttggac
    11221 gcaaccgtcg tattgagccg cgatattgcc tctttgggta tttacccggc agtcgatccg
    11281 cttgattcta cttcgcgcca actggatccg atggtattgg gtcaagagca ctacgacgtg
    11341 gcgcgcggtg tgcagtccac cctgcaaaaa tacaaagaat tgcgcgacat catcgccatc
    11401 ttgggtatgg acgaattgtc tgatgaagac aaactgaccg taatgcgtgc gcgtaaaatc
    11461 caacgcttcc tgtcccaacc gttccacgtt gccgaagtgt ttacaggttc tccgggtaaa
    11521 tatgtcgccc tgcgcgatac cattgccggc ttcaaagcca tcttgaacgg cgaatacgat
    11581 catctgcccg agcaggcatt ctatatggtc ggcagcattg aagaagcagt agagaaagcg
    11641 aaaaccttaa actaaggagg tcggcatgag catcatgcaa gttgaggtgg taagtagcga
    11701 gcagaaaatc tattcaggcg aggcaacatt tatcgttgtt ccgactgtac agggcgaact
    11761 cggtatttat ccgcgacacg agccgattat gagtttggtg cggccggggg ctttgcgttt
    11821 gaccgttccg ggcgaggata aagaggtttt ggttgctgtt tccggcggtg ttttggaagt
    11881 acagcctgat aaagtaactg tcttggcgga tgttgccgtc cgcagtgcgg agatggatcg
    11941 ggcacgtgcg gaagaggcga aaaaagccgc agaagcgggc atttcccaag ctaaagacga
    12001 taaggctttg gcggaagcgc ataaagtatt ggctgctgcg attgcacagc tcaaaacttt
    12061 ggactatatc cgttcgcaca agaaataaac ttgtttggtt tggaaaagca cgaccgtttg
    12121 gtcgtgcttt ttattttccg gatttaaatt aaagactgtg tttggatatg gcgttggcag
    12181 gcatagcctg ctttaaggta ttcaataggg cagcctgccg cctaaccgaa acatatagcg
    12241 gattaaca
//
LOCUS       DAWISC010000044        11834 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWISC010000044 DAWISC010000000
VERSION     DAWISC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11834
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.044"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(328..1398)
                     /locus_tag="WIW87_000884"
     CDS             complement(328..1398)
                     /locus_tag="WIW87_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5174065.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKV
                     AKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAE
                     AKANLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKN
                     LSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYR
                     AQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFAD
                     GVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQ
                     KAEQATVNLLRDLHSGAYVDIR"
     gene            complement(1503..3911)
                     /locus_tag="WIW87_000885"
     CDS             complement(1503..3911)
                     /locus_tag="WIW87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ5174066.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMEIEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSVEWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGEVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLVGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYITAHSLSAGR
                     NKRP"
     gene            3981..4985
                     /locus_tag="WIW87_000886"
     CDS             3981..4985
                     /locus_tag="WIW87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ5174067.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLHRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(5042..5740)
                     /locus_tag="WIW87_000887"
     CDS             complement(5042..5740)
                     /locus_tag="WIW87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5174068.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            5767..5958
                     /locus_tag="WIW87_000888"
     CDS             5767..5958
                     /locus_tag="WIW87_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174069.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            5958..7496
                     /gene="murJ"
                     /locus_tag="WIW87_000889"
     CDS             5958..7496
                     /gene="murJ"
                     /locus_tag="WIW87_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ5174070.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            7628..8584
                     /locus_tag="WIW87_000890"
     CDS             7628..8584
                     /locus_tag="WIW87_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ5174071.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     AVSAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEVSEAV
                     TLFQPAYGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8635..9417
                     /gene="trpC"
                     /locus_tag="WIW87_000891"
     CDS             8635..9417
                     /gene="trpC"
                     /locus_tag="WIW87_000891"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ5174072.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRTLAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            9482..11782
                     /gene="recQ"
                     /locus_tag="WIW87_000892"
     CDS             9482..11782
                     /gene="recQ"
                     /locus_tag="WIW87_000892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ5174073.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     RLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVYGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2381 a         3302 c         3417 g         2734 t
ORIGIN      
        1 cgcgggaatg acgatggaaa gattgttgtt gcttcggata aatttctgca gttttaaata
       61 accggattcc cgcctgctcg ggaatgacgg cataggtttt tttgtttttc cgataaatta
      121 ccacaaccca aaatcccgtc attcccgcga cggcgggaat ccagtccttt aaactccagc
      181 cattcccgat aaattcctgt tacttttcgt ttctagattc ccgctttcgc gggaatgatg
      241 aatgacggtg taaaagtaac tcgaaatcca aaaagccata ccgcccggat ttttgccgat
      301 cggtatggct ttttgcttca aaccgcctta gcggatgtcg acatacgcgc cggaatgcag
      361 gtcacgcaac aggttgacgg ttgcctgttc ggctttttgt tggaagatgt attgccgcac
      421 ggaattgcgg atacgttcct gaggtgtgcc ggcatcgcgc acttcgttca atttgatgat
      481 atgccagccg aattgggtgc ggacgggcgc gccgacctgt ccgggtttga gcgcgtggac
      541 ggcttcttca aaggcgggaa ccatcacgcc gtcggcaaac cagcccaaat ctccgccgtt
      601 gcccgcgctc gcgtcttgcg aatattggcg cgccaggctg gaaaagtctg tgccgctgcg
      661 ggcctctccg tagattttgc ggatggtgct ttccgcgccg acggcggcgt tttcgctgtc
      721 ggctttaatc aggatgtgtt gggcgcggta ttggcgcaac ggtgcgcctt cgggcagggt
      781 gatgccttgt ttttgcgcct gctcgaggaa ggcatcgatt tcagcttcgc tcacgcggct
      841 gttctgcatc actgcctgct ggcggacttt ttcggcaatg atgttgtcgg caaaatcgcg
      901 gcgttgggcg gggctgaggt ttttgagggc gggatttttt gcgacgacgg catcgatttc
      961 cgcttcgctt gcttgaatgt tgcggcgttt gcccgcctgt acaatcaggg attggttgac
     1021 aagctgcatc agcacctgtc gggacagctc ggattcgctt atctgcgcgt ctttgggcag
     1081 gttggctttg gcttcggcaa cggcttcggc aagccggcgg cgcgtgatga cttcgttgtc
     1141 ggcaacggcg gcaatgccgt ctgaaaagcg gataccgccc tgctgttgtg cgggtgcggc
     1201 ttctttttgc gccgtggcgg caactttggc aactttggca actttggcaa ctttggcaac
     1261 tttggcaact ttggcagctt tggcagcttt ggcagctttg gcagctttgg cggatgcggt
     1321 ttttgccttt tgcggtgcgg cgtggacatc ggctgctgcc agcaatgcgg cggcaatcat
     1381 cagggctttg attttcatca ttctttcctt atcgtttgaa tcctgccttt gatggcgggt
     1441 ttggggttgt cggtggaatg ccgtcttgtg cgggaacggt gctcctgcca ggtttccgac
     1501 ggtcagggtc gtttgttgcg tccggcggaa agagagtggg cggtgatata gccgggaacg
     1561 gcgacatcca tcctgtctgc ggggtttctg ccgacactgc tgaggtcttt caactgaagt
     1621 gagaaaaaga cagcgttttt gtaggtgttt tcgccggtaa cgtagcgttg ggcgtacacg
     1681 cccgcgcccc agcagccgca actgcttttg tattccgcac ccaccagcac ctctatcggt
     1741 tttttggctt caaaaccgta gttgtaacgg acgacggccg acaggttgcg cgtcagcggc
     1801 cattgtgcgg acaggtcgag ctggctgagt ttgtcgtaaa aataggaacc gtcggacttc
     1861 aggtagattt tttcgttgcg cccgtatttg tagcgggcgt tcagcacttt gccctgtgcg
     1921 ggacggtagc ttgcaccgac ggcgtagttc tcggcgcgtt tgtcgttttg gttgtagtgg
     1981 atgctgctgt cgaggatgaa gcggctgccg atgctgccgg aggcaaatgc cacccagtcg
     2041 gaacggttgc gcggtttttt gccgacgctg ccgtcaagca tcaccgcatc atccttgaaa
     2101 tagaatttct gaccgatgcc ggcgcggaaa cgctcttccc ccgtcgcgcc gtccaaaata
     2161 cggctttgca cggcggcgga aaggctgttt gcggtgttaa tcctgtcgtt gccgtaatag
     2221 aggttttcgc gaaagagctg cccgtagccg aagctgcttt ccgacgaatc gaaattgggc
     2281 aggtcgtttt gggatttggc aggaatatag ttgtagaaca ggcgcggctc gagggtttgc
     2341 aggacttctc cgccgaacat ccgcgtattc cgctcaaaag ttgcgccgct gtcgatgttg
     2401 acaatgggca gagtgcggct gacgcgtcgg gcttcttggc tgccgaagcg gttgaggctg
     2461 taataggtgg cgtgcagtcc gagtttggga cggacatagc cccagctgtt gctgaaatcc
     2521 catttgatgt cgggatagac gaccaggcgg ctgccgtctt ggcggctgtc gtggctgaat
     2581 cgggtaaatt gtgcggacac gccgatttgc gccctgccgg tgtttttacg ccactcgacc
     2641 gaaaggcgcg gcatgagggc atacggtttg tctttgtagc cgctttggtt tgccagcgtc
     2701 tggtatttca gaaccgaaag gccggcattc aggctgccgc ccgccgccct gccgccataa
     2761 tccagccata cacggcggtt gaggttgacg ttgccggcga tttctttgtt gccgtaaaag
     2821 tcgcggtagt agccgctgtc ggagacttgg ttgaaatcga cacccgcctg aagcgtgtcg
     2881 gaaatgtcgt gccgatgctg ccatttcgcc tgatagcggt tattcctgcc gcttttcttg
     2941 tcgtgcggca gccaggtcag gtcggactgg ccggcataat ccggccgcag gtagcgtacc
     3001 tgcccgtcaa agaccgcgcc gcgttcgccg atcacgctgg gcgcgaacgt ggcatcgaga
     3061 ttgggggcaa ggttgaaata atagggaacg gaaagggaaa cgccgtccga accggcggac
     3121 agtgagggaa caagcaggcc gcttttgcgg ttgccgtcaa gcgggaagtc cgcccaaggg
     3181 gtgtagaaaa tgggaacgcc gccgaacacg aaggcggcgt gtttggcaac gcctatgcct
     3241 ttttcccgat cggcttcgac agaggctgcc ttgacatacc agccggcatc gccggcggaa
     3301 caggtgttga attgggtttc cgtcagtttg taatgccctt cgcccaacat ttcggcggtg
     3361 cggctgacgc tttgcagccg ccgtccgcct tgttcgattt ccatgcggac gttgtgcgct
     3421 tcgccggtct gctgctcgag attgtaggtc agggtttcgc cccgaatcag cgtaccgtcc
     3481 tgttggaggg cgaaccggtc gcctgcggta acggtgtcgc ccgactggtc gtaatccgcc
     3541 caatcggtat tgagggtcgt ccggttgcgt tcgacgacga cgttgccttc ggcacgcacc
     3601 tgcacctgcg actgtccttc caccttgtcg gcaacaatgc gcgtatagtc ttcggggatg
     3661 gatgcttcgc cgctgcctcg gacggcggct tcggttctct cggggctgcc gctttcgtta
     3721 ctgcaaaaca ggcaggtcga accgaggctc aggctggtag gctgtatggg ttcggacacg
     3781 ctccgaacgc ttcctccggc ggtcggattg tccgtttcct ccgccgcaac ggcatcggcg
     3841 gcggcgcaat gcgtgccgaa gcagaggccc aatgccagca ccagtggttt gagtgaaaat
     3901 aaacgagcca aaatcgcccc tcaagtcggt ttgccagtta gaatagcgtt tattgtaacc
     3961 tgaaatgctt tagtactgtt atgcaacggc aaaccgaact gaaaaattgg cttcagaccg
     4021 tttatcccga acgggacttc gatctgactt ttgcggcggc ggatgctgat ttccgccgct
     4081 atttccgtgc aacgttttca gacggcagca gtgtcgtctg catggatgca ccgcccgaca
     4141 agatgagtgt cgcaccttat ttgaaagtgc agaaactgtt tgacatggtc aatgtgccgc
     4201 aggtattgca cgcggacacg gatctggggt ttgtggtatt gaacgacttt ggcaatacga
     4261 cgtttttgac cgcaatgctt caggaacagg gcgaaacggc gcacaaagcc ctgcttttgg
     4321 aggcaatcgg cgagttggtc gaattgcaga aggcgagccg tgaaggggtt ttgcccgaat
     4381 atgaccgtga aacgatgttg cgcgaaatca acctgttccc ggaatggttt gtcgcaaaag
     4441 aattggggcg cgaattaaca ttcaaacaac gccaactttg gcagcaaacc gtcgatacgc
     4501 tgctgccgcc cctgttggcg cagcccaaag tctatgtgca ccgcgacttt atcgtccgca
     4561 acctgatgct gacgcgcggc aggccgggcg ttttagactt ccaagacgcg ctttacggcc
     4621 cgatttccta cgatttggtg tcgctgttgc gcgatgcctt tatcgaatgg gaagaagaat
     4681 ttgtcttgga cttggttatc cgctactggg aaaaggcgcg ggctgccggc ttgcccgtcc
     4741 ccgaagcgtt tgacgagttt taccgctggt tcgaatggat gggcgtgcag cggcacttga
     4801 aggttgcagg catcttcgca cgcctgtact accgcgacgg caaagacaaa taccgtccgg
     4861 aaatcccgcg tttcttaaac tatctgcacc gcgtatcgcg ccgttatgcc gaactcgccc
     4921 cgctctacgc gctcttggtc gaactggtcg gcgatgaaga actggaaacg ggctttacgt
     4981 tttaaaccca atcaaaatgc cgtctgaaaa ccaagtttca gacggcattt ttcaaacggg
     5041 cttactgcgc ggctttttgt tcttcgcgta ctttgtccgc caaaaggtcg atggtgttca
     5101 taccggactc ccagtcggca aattcaactt tatatttacc gccgacgata accgtgggcg
     5161 taccgtcgat ttggaaggtt tcggtcagct cctgcatttt gtcggcgcgc gcctggcttt
     5221 cgggggactc gtaggcggca aggacttttt tgccgtcaaa ggcggtttgt tcgcccagcc
     5281 attttttgag gacttccgga ttttgcagct tgattttttg gttgaccatc gcatcgaaaa
     5341 tatggctgtt cgccacatct ttgctgtcgg cggcagccat atcgacggcg gcggcgaggc
     5401 gtgccagcgt cagcatttct ttctgccaga cgacgtgttc ggtacgcagg tacatatcgt
     5461 ctttaaaaga cttggcgtgt ttgcttaaaa caggttcgag gtgggcgcag tgcggacaga
     5521 aatagccgaa aaactcaagg acttcgactt tgcctgcctg ctgttgggga atcgggttgg
     5581 caaggacggt atagttttgc ccttcgacca gccctgccgg ggcggcggct gccgaagcgg
     5641 caggcgcgct gtcggcgggg acgctggttt ggactttgct gtcgcacgcg gcaagggcga
     5701 acagggcggc aacgccgagg gcgaggtgtc tggatttcat acggctctcc gtgatgttgg
     5761 aaatgaatgt tggaaatcaa tcggaatccg gctattttat tgcatttccg cgtgttgata
     5821 cagtttgccg ccggaaaagg acgttttcgt ttcgggaaac cgcttcagac ggcatcaaac
     5881 ccgatgccgt ctgaagcggt ttctgtcgta caatacgcgc cgttgcccca aacgggtacg
     5941 actgttgagg aacaatgatg aatatgctgg gagctttggc aaaagtcggc agcctgacga
     6001 tggtgtcgcg cgttttggga tttgtgcgcg atacggtcat tgcgcgggca ttcggcgcgg
     6061 gtatggcgac ggatgcgttt tttgtcgcgt tcaaactgcc caacctgctt cgccgcgtgt
     6121 ttgcggaggg ggcgtttgcc caagcgtttg tgccgatttt ggcggaatac aaggaaacgc
     6181 gttcaaaaga ggcggcggag gcttttatcc gccatgtggc ggggatgctg tcgtttgtac
     6241 tggtcatcgt taccgcgctg ggcatactcg ccgcgccttg ggtgatttat gtttccgcac
     6301 ccggctttgc caaagatgcc gacaaatttc agctctccat cgatttgctg cggattacgt
     6361 ttccttatat cttattgatt tcactttcct cttttgtcgg ctcagtactc aattcctatc
     6421 ataaattcag cattccggcg tttacgccca cgttcctgaa cgtgtcgttt atcgtattcg
     6481 cgctgttttt cgtgccgtat ttcgatcctc ccgttaccgc gctggcttgg gcggtttttg
     6541 tcggcggtat tttgcaactc ggcttccaac tgccctggct ggcgaaactg ggttttttga
     6601 aactgcccaa actgagtttc aaagatgcgg cggtcaaccg cgtgatgaaa cagatggcgc
     6661 ctgcgatttt gggcgtgagc gtggcgcaga tttctttggt gattaacacg attttcgcgt
     6721 cttatctgca atcgggcagc gtttcatgga tgtattacgc cgaccgcatg atggaactgc
     6781 ccggcggcgt gctgggggcg gcactcggta cgattttgct gccgactttg tccaaacact
     6841 cggcaaacca agatacggaa cagttttccg ccctgctcga ctggggtttg cgcctgtgca
     6901 tgctgctgac gctgccggcg gcggtcggaa tggcggtgtt gtcgttcccg ctggtggcga
     6961 cactgtttat gtaccgcgaa tttacgctgt ttgacgcgca gatgacgcag cacgcgctga
     7021 ttgcctattc tttcggttta atcggtttga tcatgattaa agtgttggca tccggctttt
     7081 atgcgcggca aaacatcaaa acgcccgtca aaatcgccat cttcacgctc atctgcacgc
     7141 agttgatgaa ccttgccttt atcggcccac tgaaacacgt cggactttcg cttgccatcg
     7201 gtctgggcgc gtgtatcaat gccggattgt tgttttacct gttgcgcaga cacggtattt
     7261 accaacctgg caagggttgg gcggcgttct tggcgaaaat gctgctctcg ctcgccgtga
     7321 tgggcggcgg actgtgggcg gcgcaggctt gcctgccgtt tgaatgggta cgcgccggcg
     7381 gaatgcggaa agcggggcag ctctgcatcc tgattgccgt cggcggcgga ctgtatttcg
     7441 catcactggc ggctttgggc ttccgtccgc gccatttcaa acgcgtggaa aactgaccga
     7501 tgccgatatc ctttttcggc aaacgctttt ccacgcctat attcgggcgc gcatccccca
     7561 cgtccgcacc agccggacgg gctgccgcat catcaatgcc gtctgaaaac aagaaaaccg
     7621 ataccttatg attttaacac cgccggacac gccctttttc ctccgcaacg gcaatgccga
     7681 cacgattgcc gccaaattcc tgcaacgccc cgcacccaca taccgccgcg agctgcttcc
     7741 cgacagcacg ggtaaaacca aagtcgccta cgatttttca gacggcattt cgcccgatgc
     7801 gccgctggtc gtgctgtttc acggtttgga aggaagcagc cgcagccatt acgcggtcga
     7861 actgatgctc gcggtacgca gccgcggttg gcacggcgca gtcgtccatt tccgcagctg
     7921 cggcggcatt gccaacaccg ctccggtgtt ctaccacttg ggcgataccg ccgaaatcgc
     7981 ctttactttg gacacgcttg ccgcgcgtta ccgtgaaata tacgccgtcg gcgtatcgct
     8041 gggcggcaac gcgctggcaa aatatttggg cgaacggggc gaaaacgcgc tgccgcaagc
     8101 cgctgccgcc gtatccgccc ccgtcgatgc agaggcggca ggcagacgct tcgacagcgg
     8161 catcacgcgg ctgctctaca cgcgctactt cctccgcacc ctgataccca aagcacggtc
     8221 gctccacggt tttcagacgg catttgccgc agggtgcaaa acactgggcg agtttgacga
     8281 ccgtttcacc gcaccgctgc acggctttgc cgaccggcac gactactacc gccaaacttc
     8341 ctgcaaaccg ctgctcaaac acgttgccaa accgctgctc ctgctcaatg ccgccaacga
     8401 ccccttcctg ccgcccgaag cgctgccccg cgcagacgaa gtatccgaag ccgttaccct
     8461 gttccagccg gcatatggtg gtcatgtcgg ctttgtcagc agcaccggcg gcaggctgca
     8521 cctgcaatgg ctgccgcaga ccgtcctgtc ctatttcgac agcttccgca caaacaggcg
     8581 ttaacggttt gatgctaata ttcccccttt ccccagacga atacggaacc cgacatgacc
     8641 gacatcctca ataaaatcct tgccaccaaa gcacaggaag ttgccgctca aaaagccgcc
     8701 gtcaacgccg aacacatccg cacacttgcc gcagaagccg cgcccgtccg cagcttcatc
     8761 gattcgatac gcggcaaaca ccgcctaaac ctgcccgccg tcatagccga aatcaaaaag
     8821 gcaagcccga gcaaagggtt aatccgtccg gacttccgcc ctgccgagat tgcacgcgcc
     8881 tatgaaaacg ccggagcggc gtgtttgtcc gtactgaccg acgaacccta tttccaaggt
     8941 tcgcccgaat acctcaaaca ggcgcgcgaa gccgtatcgc tgcccgtgct gcgcaaagac
     9001 ttcatcatcg acgaatacca ggtttatcag gcgcgcgcct ggggggcgga tgccgtcctg
     9061 ctgattgccg ccgcactgga acgggaacaa ttggaacgct ttgaagcggt ggcgcacgaa
     9121 ttgggcatga ccgtcctgct cgagctgcac gatgaaagcg aattggaaaa atgccgcaac
     9181 ctgaccacgc cgttgtgggg tgtaaacaac cgcaacctga ggacttttga agtctccctc
     9241 gaccaaaccc tgtcgctgct gcccgcgctg gaaggcaaaa ccgtcgttac cgaaagcggc
     9301 attacaggca aggcggatgt ggaatttatg caatcgcgcg gcgtgcatac cttcctgatc
     9361 ggcgaaacgt ttatgcgtgc cgacgatatt gaagcagaag tgggcaaact attctaaatc
     9421 ccgatttcag acggcatatt gccgccgacc gatcaacacc cttaccgagc acggaaccat
     9481 catgcaccga ccgaccgcca aacagattct gcacgaagta tttggttatc ccgaatttcg
     9541 cggcaggcag gaggctgtca tcaatacttt ggcaggcggc gggagtttga cggtgctgat
     9601 gccgacgggc ggtggcaagt ctttgtgtta ccagattccc gcgctgatgc gcgaaggcgt
     9661 ggcggttgtc gtatcgccgc tgattgcgct gatgaacgac caagtggcaa acctgcacgc
     9721 cgccggcatc gaagcggcgg cagtcaacag cggcacatcg gcagacgagg cgcgcgagat
     9781 tgccgaccgg cttgcccaag gccgtctgaa gctgctttat gtcgcgccgg aacgcttggt
     9841 taccgaccgc tttttgcgtt ttctcgacca acaaaccgtc agtctgtttg ccattgatga
     9901 ggcgcattgc gtcagccaat ggggacacga tttccgccct gaatatcaac agctcggcat
     9961 gcttgccgaa cgctatccga acgtcccgcg catcgctctg accgctaccg ccgatgccgc
    10021 cacgcgcgcc gacatcaagc attatctgca cttggacgat gcgcccgaat ttgtctccag
    10081 ctttgaccgt ccgaatattt attatcaggt tatcgaaaaa aacaacggca aaaaacaatt
    10141 gctggatttc atccgcaaag aaatgacggg gcaaagcggc attgtgtatt gcctaagccg
    10201 caaaaaggtg gaagatgtgg cgcagttttt gcgtgaaaac ggattaaacg cgattccgta
    10261 tcatgctggt ttgagcatgg acgtacgcga ggaaaaccaa cgccgcttta cgcatgaaga
    10321 caatattatc gtggtggcga ccgtggcgtt cggcatgggc atagacaaac ccgacgtgcg
    10381 ctttgtcgcc catctcgata tgccccagag tgtcgaacat ttctatcaag aatcggggcg
    10441 cgccggacgg gacgggctgc cggccgcaag ctggctgtgt tacggtttga acgattgggt
    10501 gttgctgcgc gaacggattg ccgaaggcaa cagcgacgag gtgcaaaagc aaatcgaaat
    10561 gcaaaaactc gatgccatgc ttgccgtctg cgaaaccgcc gcctgccgcc gcgtactgct
    10621 gctcaaacat ttcggcgaag catccgaacc ctgcggccat tgcgacaact gcctgcatcc
    10681 gcccgtacgg tttgacggca cggtgttggt gcaaaaatta ctcagctgcg tgtaccgcgc
    10741 cggacaacgt tttgccgccg gttacatcac caacatttta cgaggtaaaa gcgacgattg
    10801 gatacgcggc aaccggcacg aacaactgtc cacattcggc atcggtacgg agttgtccga
    10861 caaagaatgg cgcagcgtca tccgccagtg tatcagcctc ggctacctca ccgtcaacat
    10921 tacccgatat caggcattgc aactgaccga agccgccaaa aaagtcctca aaggcgaaac
    10981 cgaagtgatg ctgcgtccgc tcaagcgcga caagcccgcc gcccgcaccc tcaaagacaa
    11041 ctggctgcgt accgaacgcg aagaacgcct gtggcaggca ttgcgcgttt ggcgtatgaa
    11101 acaggcagaa gccgaaggca tccccgccta tatgattttc ggcgacaaaa ccctgcgcga
    11161 ccttgtcgaa aaaatgccgc aagacctcaa cggtctgcac gacatctacg gtttgggcga
    11221 agccaaaatc gaccgtttcg gacacggcat cctcgaagtc tgccgcaacg ccgccggctt
    11281 cagccgcgac gcggtcatcc gtccgcaaac cgaacgcgaa caacaactgc gtcaaaaact
    11341 cgaagcctgg cggtatgaac aggcaagggc ggaaaactgc gccctgcatg ccgtcctctc
    11401 cgacgaaagc cttgccgata tgcttgccga tacgcccgaa accgaaaccg acctcgaagg
    11461 cgtgtacggc ttgggcagcg tacgcgccgc caaatacgga cgggacatcc tcgccgtctg
    11521 ccgtccgttt tcagacggca tcgatgaaac cgccaaacgc aaacgccgcc tgatgcgcgc
    11581 cctgatccaa tggtgcggcg aaacggcaaa acacgaacag tccgaaccct accgcattct
    11641 cagcaaagcc gcactgcgcg ccattgccgc caaacagccg gaaggcttgg cggagcttgc
    11701 cgccgtatac ggcgtaggcg aagaaaaagc cgcacgttac ggtgcggcgg tgttggcggt
    11761 attggaacga aatgccgtct gaagcccgtt accaagtttc agacggcatt gcctctattt
    11821 aaaaattcct gttt
//
LOCUS       DAWISC010000045        11797 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWISC010000045 DAWISC010000000
VERSION     DAWISC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.045"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..801
                     /locus_tag="WIW87_000893"
     CDS             <1..801
                     /locus_tag="WIW87_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5174074.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQI
                     LLDFLSKDDTDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIREKLFFERGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRK
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLLGVDITGRILKAIRGNI"
     gene            842..1372
                     /locus_tag="WIW87_000894"
     CDS             842..1372
                     /locus_tag="WIW87_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ5174075.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1406..1522)
                     /locus_tag="WIW87_000895"
     CDS             complement(1406..1522)
                     /locus_tag="WIW87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ5174076.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1541..1728)
                     /locus_tag="WIW87_000896"
                     /pseudo
     CDS             complement(1541..1728)
                     /locus_tag="WIW87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1747..3342)
                     /locus_tag="WIW87_000897"
     CDS             complement(1747..3342)
                     /locus_tag="WIW87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ5174077.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WIW87_000898"
     CDS             complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WIW87_000898"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ5174078.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRALRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WIW87_000899"
     CDS             complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WIW87_000899"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ5174079.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WIW87_000900"
     CDS             complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WIW87_000900"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ5174080.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WIW87_000901"
     CDS             complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WIW87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ5174081.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WIW87_000902"
     CDS             complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WIW87_000902"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ5174082.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRNANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WIW87_000903"
     CDS             complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WIW87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ5174083.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WIW87_000904"
     CDS             complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WIW87_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ5174084.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WIW87_000905"
     CDS             complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WIW87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ5174085.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
BASE COUNT         2517 a         2787 c         3585 g         2908 t
ORIGIN      
        1 cggacaccaa atatcaaata tatatatcag gataaccaag ggttgtcgga ggcgagaaat
       61 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
      121 aactgtcaga ttttgctgga ttttcttagt aaagatgata ctgatatgcc ggatgtggtg
      181 tttttaaatg cggttaaata tgataaggga agtgtttcat attttggcga agattatcag
      241 cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga
      301 aaatttccgg gttcggcgtg gaataagatt ataaaaagag aattgattat tagagaaaaa
      361 ctgttttttg aaaggggaat ttattctgaa gatatcgaat ggtcaatgag gttatttaat
      421 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa
      481 gattctatta cgggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
      541 aaaaatgcgg aaatggaatt taatagggat atatcgagtt atctttattc ttttctttcc
      601 tacgaatatc tcgttttgct ttttataatg acgagtaaaa atatagagtg tgatgctgat
      661 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
      721 aagctgatat tcccgataat cacattactc ggggtcgata ttacaggcag gattttaaaa
      781 gcaatcaggg ggaatattta ataaatcctt taacaatata taccttaccg aaggaggaaa
      841 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa cctgtatgat
      901 agacgaagcc tgcgtcgtca ttggcgaagt gtcgcttgcc gaagatgttt ccgtgtggcc
      961 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     1021 ggacggcagc gtcttgcacg tttcccacaa aaccgccgcc aaacccgaag gatcgccgct
     1081 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     1141 caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga
     1201 agtgatgatc ggcgcgggca gcctcgttcc gccgcgcaaa cgcttggcgg gcggctatct
     1261 ttatgtcggt tcgccggtca gacaggtgcg cgtgctgacc gatgaggaaa aagccttttt
     1321 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     1381 cggcgttctt gcgtcagccc caaattcatg cggatgggac gcatccgata acggtatccg
     1441 atgcgccttg attttgaccg tctgcgtttg aattgcaggc aaaaatgccg tctgaaagcc
     1501 ttttttcggg ttcagacggc attttattgc cgattgtttt ttaaagtttg accgaatgtt
     1561 cgcgcgtttc gtggaacacg atgtccggcc agcgttcttg cgtcagccct aaattcatga
     1621 ggacgggatg cccgataacg gtatccgatg cgtcttgatt ttgatcggtg catttgagtt
     1681 gcaggcaaaa atgccgtctg aaagcctttt ttcgggttca gacggcattt tatcgccgat
     1741 tgctttttac agtttgaccg aatgttcgcg tgtttcgtgg aacacgatgt ccggccaacg
     1801 ttcttgcgtg agtcccaaat tcacgcggtt gggggcgagg taggcgaggt tgccgcctgc
     1861 gtcgatggcg aggttgcccg cgttggcttt ttcaaattca gccagttttt tcttgtcgtc
     1921 gcacgatacc cagcgcgccg accagatgga tgcgctgtcg aacacggctt ctacgccgta
     1981 ttcgttggcg aggcgcgagg taacgacttc aaactgcaac acgccgaccg cgcccaaaat
     2041 caaatccgcg ccgctcatcg gtttgaacac ctgcaccgcg ccttcttcgc cgagctgttg
     2101 caagcctttt tgcagttgtt tgattttcag cgggtttttg atgcgtacgc tgcggaacag
     2161 ttcgggtgcg aagaatggga tgccggtgaa cgccagttgt tcgccttcgg agaagctgtc
     2221 gccgatttgg atgttgccgt ggttcgggat gccgataatg tcgccggcgt aggcttcttc
     2281 aaccagctcg cggtcgtgcg acatgaaggt aaccacgctg gaggcggcga tttcgcggtt
     2341 gatacgcagg tgtttcatct tcatgccgcg ctcgaatttg ccggagcaga cgcgcaagaa
     2401 ggcaatacgg tcgcggtgtt tcgggtccat attggcttgg attttgaaga taaatccgga
     2461 aaacttcggc tcgtccggct cgaccatacg tacggtcgcg tcgcgcggtt tcggcgcggg
     2521 cgcccagtca atcaatgaat tgaggatttc ctgaataccg aagttgttaa tcgcagagcc
     2581 gaagaatacg ggcgtgagtt cgccggcgag gaattcgtcg agattaaact cgttggaagc
     2641 cgcctgcacc aattcgattt cgtcgcgcaa ctgctggatt tccaacggaa agcgttgttc
     2701 caattcagga ttatcgatgc ctttgatgat gtcgaactcg tgcggcaggc gttcgccgcc
     2761 agcttcaaag agataaattt catcgttcag gatgtggtac acgcccttga agtttttgcc
     2821 cataccgatc ggccaggtaa cgggcgcgca gcggattttt aaaatgtttt ccacttcgtc
     2881 caaaagttcc agggaatcgc gcacttcgcg gtcgtatttg ttcataaacg taacaatcgg
     2941 tgtatcgcgc aggcggcaga cgtttaagag cttgatggtt tgcgcttcca cgccttttgc
     3001 cgcgtcgatg accattaatg cgctgtccac ggcggttaaa acgcggtagg tgtcttcgga
     3061 gaagtcttgg tgtcccggcg tgtccaagag gttgacggtg tggtctttgt aatcgaactg
     3121 catcacactt gatgccacgg aaatgccgcg ctgcttctcg atttccatcc agtcggaagt
     3181 ggcgaatttg ccggttttct tgccttttac cgtacccgcg ctctgaatcg cgcccgaaaa
     3241 cagcaagagt ttttcagtca acgtggtttt acctgcgtca gggtgggaga tgatggcaaa
     3301 cgtgcggcgg cggcgcactt ggtcgaggat ttcttgggac atggttttct ttgcaaaaag
     3361 gttcaggccg cttttcagac ggcccggaca gtgtttgaga cggcgaaatt gtacaaaaaa
     3421 atgcctgata attcaatgtt ggaggcggtc agtgcgtgct gccgtaaatc tctttttcgt
     3481 ctttcaggac ggcatcggcg gtttcccacg cgctgccacg ccagactttg taaaagcagc
     3541 tttctcgccc ggtgtggcag gcgatgccgc cgttttgggc gatgagcatc acaatggcgt
     3601 cgccgtcgca gtcgaggcgc agtgcgcgga ctttttgcgt gtgtcccgac tcttcgccct
     3661 tcatccattg tttttggcgc gaacggctgt aatagtgggc aaagccggtt tcgacggttt
     3721 tttgcagggc ttcggcgttc atccacgcca ccattaaaat acgtttggtt tcggcatctt
     3781 gggcgatggc gcaaaccaaa cctttttcgt caaatttgac ggcttcaagc aggtttttat
     3841 ccatatttcc tttcagacgg catagtcgag gcggtcagag gcgcacttcg atgccggctt
     3901 cgcgcatagc gcgtttggct tcgcggatgg cgatttcccc gaaatggaaa atgccggcgg
     3961 caagtacggc atcggctttg ccttcggtta tgccttcaat caggtgccgg acattgccga
     4021 ccccgccgga ggcgatgacg gggatgtcga cggcttcggc aacggcgcgg gtcagcggca
     4081 ggttgaaacc ctgtttcgta ccgtccctgt ccataccggt gagcaggatt tcgcccgcgc
     4141 cgcgtttttg catttcgacc gcccattcca ccgcatccaa accggtcgga tttcgcccgc
     4201 cgtgggtaaa gatttcccag cgtgtgtttt cggggttggc ggctttggca tcgacggcgg
     4261 cgacgatggc ttgcgaaccg aaaaatccgg cggcttcgtc aattaaatcg ggacgggtaa
     4321 cggcggcggt gttgatgctg actttgtccg cgccggcatt gagcaggcgg cggatgtcgg
     4381 caacggtgcg tacgccgccg ccgacggtca gggggatgaa gacttgtccg gcaacctctt
     4441 cgatgatgtg caggatggtg tcgcggttgt cggatgaggc ggtgatgtcg aggaaggtca
     4501 attcgtccgc gccttcgccg ttgtagcgtt tggcggcttc gacggggtcg cccgcgtcgc
     4561 gcaaaccgat gaagttcacg cctttgacga cgcgcccgtc ttttacgtcg agacagggga
     4621 tgatgcgttt tgccagtgcc ataatcggat gcctttagtc gagggaatct gccagttgct
     4681 gcgcttgggc aaaatcgatg ctaccctcgt aaatcgcgcg gccggtaatc gcgcctgcta
     4741 cgccatgttt ttcggcggca cacagggcgc ggatgtcgtc caagccggtc agtccgccgg
     4801 aggcgatgac gggaatgcgg acggtttggg cgagtttgac cgtcgcgtcg atgttcacgc
     4861 cgctcatcat accgtcgcgc ccgatgtcgg tgtagatgat gctgttgacg ccgtcgtctt
     4921 caaagcgttt tgccaaatca attacatgat gcccggttac ggttgcccag ccgtcgatgg
     4981 cggccatacc gtctttggca tccagcccga caataatcct gccggggaag gctttgcacg
     5041 cctcgcgcac gaagtcgggg tttttgaccg ccgccgtgcc gataatcacg tcgtttaagc
     5101 ccaaatccaa atattgtccg atggttttca aatcgcgtat gccgccgccg agctgtacgg
     5161 ggatgtcttt ggcgacagcg gcaaggatgt ctttgatggc gggcaggttt tgcggaacgc
     5221 cggcaaacgc gccgttcaaa tctaccagat gcaggcggcg cgcgccttgt ttgaaccagt
     5281 gcagcgcggt ttcggcgggc gaatcggaaa agacggtcgc ctcttccatc agcccttgtt
     5341 tcaggcggac gcagcgtcct tctttcaaat cgatggcggg tatcagcagc ataatttttc
     5401 tccttgtgcg gggccgtgtc cggcttacca gtttaaaaag tttttcaaca tcgtcagccc
     5461 ggcatcgtgg cttttttcgg tgtgaaattg cgtggcgaat acgttgtctt tgccgacgat
     5521 gcaggcaaac ggggacgggt agtcgctttc gcccaatatg gtttcgggat tttcgggggc
     5581 gaaatagtag ctgtggacga agtaaaaacg cgtgtcttgg ggaatatctt taaacagcgg
     5641 gtggttttgg gtttggcgca cggtgttcca gcccatatgc gggactttca gacggcatcc
     5701 ctgcgggtcg cggaggtcgc gctcaaagcg tctgactttg ccgccgaacc agcccaagcc
     5761 gtcggtgttt ccttcttcac tgtggtcgaa taaaagttgc gcgccgacgc agattccgaa
     5821 aaacggtttg ttttttaagg catctttgac tgcctcgtcc aaaccgtctc gttttaatgc
     5881 cgccatacag tcgggcatcg cgccctgacc gggaaaaatg actttgtcgg cgcgggacac
     5941 gcggtcgggg tcgccgctta aaaagatttc ggtatttttt ccggcaagct gccccgccgt
     6001 ccggacggat ttcaatacgg aatgcaggtt gcccataccg taatcgataa tggcggtttg
     6061 catggcttcc tcctcttttt ttgcaatatg gctgcgattt taacaaacaa atgtgccgtg
     6121 ctgataaaaa tgccgtctga aaacgggagt ctgtcttcag acggcatagg gtttaaaccc
     6181 ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg gcgatggtaa
     6241 acacgatatc ttctaccagt gcgccgcggg agaggtcgtt gaccggttta cgcaggcctt
     6301 gcagcagcgg gccgacgctt aagacgttgg cgttgcgttg gacggcttta taggtgcagt
     6361 tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc gggctgcccg
     6421 gagctttgga tttgcccaca cccggcacgg ttgccgcatc atattgcagc gggccgtcga
     6481 tggcgaggtc ggggcgtttt tcccgggcaa gtttggttgc ttcgatgacg gtatcgacat
     6541 cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc gggtcgatgc
     6601 cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt tgcgcggtcg
     6661 ggttcggatt aaccgcgcag tcgccgaaga cgaggacttg gttgggcagc agcataaaga
     6721 atacgctgga cacgaggctt gcgcccggtg cggttttaat cagttgcaaa gcggggcgga
     6781 tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca ttttgcgcca
     6841 tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct tcgggtgtca
     6901 ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc aatgaggcgg
     6961 gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca acggcttcga
     7021 cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg cagatggcgg
     7081 cggcttggac ggtgcggggt tctgcgccct caggcaggac gatgcgtttg tcggcttggc
     7141 gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt gcttcgcggc
     7201 ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc aggccggttt
     7261 tttcggctgc cgcttcggca acggaggctg ccgcgccgtc cacgacaaaa ccttccaata
     7321 cgcccggcgc ggcggcgaag aactgtttgg caaggttcac ccgatttgcc agttcgtcgg
     7381 catcggcgtt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac aatgccagtt
     7441 cgacgttttt gcctgcgagg tagattttgt cggcatcggg cgcgatgcct tcgatgacga
     7501 ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag tcgtcgcttt
     7561 tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaatt tgtgcgtcca
     7621 gtgcttttgc aaaggcttgt gcggcggcgg aggcgtccag tccggcagat acgggtacga
     7681 tgagtacttt tgccatgata tatcctttcg tatgctgcgg tgtgcggcat atgtggttgg
     7741 aaggggcggc atataggcag aaacggctgc ctgcgtgccg tgcgtgccgt gtttggcttg
     7801 aggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa tatccgcagg
     7861 cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc gggaccgtcc
     7921 aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag gttgagcgtg
     7981 aggacggttt gtccgacccg gaggctgatt tcggtatgcc gcgctttggg cgtggttttg
     8041 agaaccacgg cgtgaatgga ggcggtgggt gcggaatggg ggtgaaggct gaactgttcc
     8101 ggacggatga gcagtgtgcc gcgtgtgcct gcgggtgcgc cgctttgaac gggcaggcgg
     8161 cccaatccgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac gatgccttcg
     8221 ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg agggcttgca
     8281 gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg cagggcttct
     8341 tcgcggtcgt ggctgacgaa aacggcagat ttgccgttgg cgcgcagggc ggcaatcatg
     8401 tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc gtccaacaaa
     8461 atcagttcgg gatcgggtgc gagggcgcgg gcgagggcga cgcgctgttg ctgtccgccc
     8521 gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc caacatagct
     8581 tcgatgcgct gccgctcttg cgccgtcttg cctttgccgt tgcccagccc gtaggcggtg
     8641 ttgcggtaaa cggtcaggtg ggggaacagc acaccttcct gtacgacata acccaaacgg
     8701 cgttcgcgga cggggaggtt ggtatttttc gagaagatgg ttctgccgga aagcgaaatt
     8761 tcgccaaaat cgggttgttc aaaaccggca aggcagcgta aaagggtggt tttgccgcag
     8821 ccggacgcgc cgacgataaa gaggatttcg cccgggtcga ggctgagcga aatgtcgttt
     8881 aaaactgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc ggcggtcatg
     8941 gcggtacttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat acggggatgc
     9001 cggaaaataa taccagcatc agcgcgtaag gggtggcggc ggcgtattgt gcgtccgatg
     9061 tgtattccca aacggcggtg gagagtgtgt ggacatcgtc ggtggtcagc agcagggtgg
     9121 cggtcagctc tttcatcagt ttgaggaaga cgagtgcgaa tgcggcggta atgccgggca
     9181 ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc aatgttgcgc
     9241 cgacctgttc catccctttt gggagttgtt ccaaggaagt cctcagggtg gtttgcgcca
     9301 tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg taaacggcag
     9361 gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg gggacggcgt
     9421 gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag cgaaccgatg
     9481 cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc gatacgctta
     9541 aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa gtgccgacca
     9601 tcatccaatg tatcaatacg ccaaagggga taataatgcc caaagtcaac aagctgctta
     9661 aaaaaacaat cgcgccaatc tgaccgggca gtttgagggt tttgacggga taaggacggg
     9721 caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt tctccaaata
     9781 cgacgatgcc gcacaccgcc attaaaacag cggaaagcag ggcggcggta ttgttgttgt
     9841 aggacatttc gtattcttgg aaaatggcgg tggtaaaagt ggggtagttc aaaatggata
     9901 ccgcgccaaa ttcgaccagc atatgcaggg caatcagtaa cacgctgctg ccgatggcgg
     9961 gtttgagctg ggggaggatg gcggaaaaaa aggtttgcag gcggcttttg cccaaggaca
    10021 ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg ggcaggtagg
    10081 cgagcgggaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct tcgacacgga
    10141 aggtcaggct gatccaggtg aaacagctga caaatgcggg gatgcacaaa ggcagggtga
    10201 ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa agggcgcagg
    10261 caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag gtgttggaga
    10321 gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg ccgacctgcc
    10381 acgaacgcat agcgacatat aaaaaaggca gggtaagcgg tagggcaatc agtaggatga
    10441 ggccggtaag ccaaatgggt atttttttag gagacatagt gttttttatc ggcaaaacgg
    10501 gcggacagta taaatgtcca cccgtttgac aatccgaaaa cggcttattt cataccggct
    10561 tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga cacttggggt
    10621 gcttccaact tggcgatggg ttccaaattg aaagtggata ccacgtgcgg attcaaagga
    10681 tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgctggcgag gaaggcgacg
    10741 aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc ggaataggta
    10801 acgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac attttgtacg
    10861 cctttttcac gcgcaaaagc gtgccagtag tagttgttga tgagggcggc atcgatttcg
    10921 ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc gtattctttc
    10981 agacctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat ggcgacaacc
    11041 tgttccaaga acgcgccgga agtgggggcg taaccgatgc ggtttttcca tttcggcgtg
    11101 gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt gtcgtaaacg
    11161 acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac cggcacgccc
    11221 ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt ggcggcggaa
    11281 agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct tcggctgcct
    11341 tcttctttga tttgaccggc aagctggtcg cctttggcgc tgttgagttt gactttgatg
    11401 ccggtagccc gggtaaaggc atctgcaacg gcttgtgctg cttctttgtg ttggccgttg
    11461 tacacggtaa tgtctgccag cgcgggggtt gcggcggtca gggctgcggc aagcagtgcg
    11521 tatcggatag atgttttcat atcgattttc tcctaatgaa tgagagtgta taccttgtta
    11581 agacataacg gtgtgtagtg tattccttct tttttataaa tgcaaataat tattttttaa
    11641 atttgttgtt gtccgatccg gttattgttt gttctgactt gtattttttc cgtgagtctc
    11701 gcccgtaagg cggaagtggc gggcaatgcg tggcggaatg tgggtaaagg cggcattttg
    11761 atttgtcgga atgcttgaga acccctctct ttaaaac
//
LOCUS       DAWISC010000046        11644 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWISC010000046 DAWISC010000000
VERSION     DAWISC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.046"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(339..761)
                     /locus_tag="WIW87_000906"
     CDS             complement(339..761)
                     /locus_tag="WIW87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ5174086.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WIW87_000907"
     CDS             complement(879..1550)
                     /locus_tag="WIW87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ5174087.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..2192)
                     /locus_tag="WIW87_000908"
     CDS             complement(1566..2192)
                     /locus_tag="WIW87_000908"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ5174088.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIHNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="WIW87_000909"
     CDS             complement(2299..3117)
                     /locus_tag="WIW87_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5174089.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="WIW87_000910"
     CDS             complement(3376..3816)
                     /locus_tag="WIW87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ5174090.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="WIW87_000911"
     CDS             complement(3879..5285)
                     /locus_tag="WIW87_000911"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ5174091.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEASQLSNPTVLALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5456..6571
                     /gene="lptF"
                     /locus_tag="WIW87_000912"
     CDS             5456..6571
                     /gene="lptF"
                     /locus_tag="WIW87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ5174092.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6568..7638
                     /gene="lptG"
                     /locus_tag="WIW87_000913"
     CDS             6568..7638
                     /gene="lptG"
                     /locus_tag="WIW87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ5174093.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7853..10438
                     /gene="acnB"
                     /locus_tag="WIW87_000914"
     CDS             7853..10438
                     /gene="acnB"
                     /locus_tag="WIW87_000914"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ5174094.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10580..11575
                     /locus_tag="WIW87_000915"
     CDS             10580..11575
                     /locus_tag="WIW87_000915"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ5174095.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2678 a         3242 c         3024 g         2700 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcggata tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggtatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaaacaca tcccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcagaaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcaataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcgag tatcggcaca
     1741 accccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggaaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttc
     1981 tccatcatgg cggtatcgaa ggcaagccgg gatgtaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tgcggacgtt gtgaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccagaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccgt
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cggaccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa gggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgagc tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cggacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ctcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgaacatt tggcaggata
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccggtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgtgc ccatggatag tcttcggtaa agtaggatag gaatgttgcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atgtttggaa tgtcggcaaa gttggatttg
     4081 agttggtctt tgtaggtctc aaagagcggc agttgccatg ctttgtcgtc cacgttgtag
     4141 gaagcggcaa gcaggctgtc gatcaaatcc tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg caacaataca cgcgccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgct cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc ggcatcggtg
     4321 ttcaacactt cgatggtcag ccctttcata cttttcacga catcgcccgg tttgtttgcc
     4381 gcgccggaag gcatattttc acaagtggcg acgacggcaa tcaggttaat cggcagttgc
     4441 agtttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat atcaaacttc
     4501 atttcgtcca tgttcaggcc gggcttgagg gagatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca ataccacagg cgcggcttct ttgtcggctg caccgaaata gctcagttcg
     4621 accaaatagg gggcttccgc gctgcctttg gcgaccgacc aaaacgaacc catgttttct
     4681 ttgatgtagt ctttttcgat gattttggcg tgcgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttgcc caagtcgcgg
     4801 cagaggcttt gtccgtaaac ttgcgcttcg gcgacgcgca aggcttcttt gacggcggct
     4861 tcgtgcgcgg tatggaacac ggcagtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc attgccgaac gcaatcgcaa acgcttcggc aacggctgca
     4981 gcctgcgctt cttcaaagac gtgaacgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa attttgacct
     5161 tcttcaagcg aagacaaaag ggcaaggacg gtcgggttgc tcagttgcga tgcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc cctgaccgcg attctatgat
     5461 ttatcaaaga aacctcatca aagaactctc ttttaccgcc gtcggcattt tcgtcgtcct
     5521 cttggcggta ttggtctcca cgcaggcaat caacctgctc ggccgtgccg ccgacgggcg
     5581 tgtcgccatc gatgccgtgt tggcattggt cggcttctgg gtcatcggta tgacgccgct
     5641 tttgctggtg ttgaccgcat ttatcagtac gttgaccgtg ttgacccgct actggcgcga
     5701 cagcgaaatg tcggtctggc tatcctgcgg attggcattg aaacaatgga tacgcccggt
     5761 gatgcagttt gccgtgccgt ttgccgtttt ggttgccgtc atgcagcttt gggtgatacc
     5821 gtgggcagag ctacgcagcc gcgaatacgc tgaaatcctg aagcagaagc aggaattgtc
     5881 tttggtggag gcaggcgagt tcaacagttt gggcaagcgc aacggcaggg tttattttgt
     5941 cgaaaccttc gataccgaat ccggcatcat gaaaaacctg ttcctgcgcg aacaggacaa
     6001 aaacggcggc gacaacatca tcttcgccaa agaaggtaac ttctcgctga acgacaacaa
     6061 acgcacgctc gaattgcgcc acggctaccg ttacagcggc acgcccggac gcgccgacta
     6121 caatcaggtt tccttccaaa aactcaacct gattatcagc accacgccca aactcatcga
     6181 ccccgtttcc caccgccgta ccattccgac cgcccaactg attggcagca gcaacccgca
     6241 acatcaggcg gaattgatgt ggcgcatctc gctgaccgtc agcgtcctcc tactctgcct
     6301 gcttgccgtg ccgctttcct atttcaaccc gcgcagcgga catacctaca atatcttgat
     6361 tgccatcggt ttgtttttaa tttaccaaaa cgggctgacc ctgctttttg aagccgtgga
     6421 agacggcaaa atccattttt ggctcggact gctgcctatg cacattatca tgtttgccgt
     6481 tgcactcatc ctgttgcgcg tccgcagtat gcccagccag cccttctggc aggcggttgg
     6541 caaaagtctg acattgaaag gcggaaaatg aacctgattt cacgttacat catccgtcaa
     6601 atggcggtta tggcggttta cgcgctcctt gccttcctcg ctttgtacag cttttttgaa
     6661 atcctgtacg aaaccggcaa cctcggcaaa ggcagttacg gcatatggga aatgctgggc
     6721 tacaccgccc tcaaaatgcc cgcccgcgcc tacgaactga ttcccctcgc cgtccttatc
     6781 ggcggactgg tctccctcag ccagcttgcc gccggcagcg aactgaccgt catcaaagcc
     6841 agcggcatga gcaccaaaaa gctgctgttg attctgtcgc agttcggttt tatttttgct
     6901 attgccaccg tcgcgctcgg cgaatgggtt gcgcccacac tgagccaaaa agccgaaaac
     6961 atcaaagccg ccgccatcaa cggcaaaatc agcaccggca ataccggcct ttggctgaaa
     7021 gaaaaaaaca gcattatcaa tgtgcgcgaa atgttgcccg accatacgct tttgggcatc
     7081 aaaatttggg cgcgcaacga taaaaacgaa ttggcagagg cagtggaagc cgattccgcc
     7141 gttttgaaca gcgacggcag ttggcagttg aaaaacatcc gccgcagcac gcttggcgaa
     7201 gacaaagtcg aggtctctat tgcggctgaa gaaaactggc cgatttccgt caaacgcaac
     7261 ctgatggacg tattgctcgt caaacccgac caaatgtccg tcggcgaact gaccacctac
     7321 atccgccacc tccaaaacaa cagccaaaac acccgaatct acgccatcgc atggtggcgc
     7381 aaattggttt accccgccgc agcctgggtg atggcgctcg tcgcctttgc ctttaccccg
     7441 caaaccaccc gccacggcaa tatgggctta aaactcttcg gcggcatctg tctcggattg
     7501 ctgttccacc ttgccggacg gctcttcggg tttaccagcc aactctacgg catcccgccc
     7561 ttcctcgccg gcgcactacc taccatagcc ttcgccttgc tcgccgtttg gctgatacgc
     7621 aaacaggaaa aacgttgaac caatgccgtc tgaacctctc ttcagacggc atttgttttc
     7681 attgacacat tcccacagac agatagccgt tccctattac attacctgtc ataacagttc
     7741 catttttgtt aaaactagtc tatgatagga tagcggtaca aatattgttt acaatattta
     7801 acgcaaatca tttgcaaccc gacaaaagaa aaacagaaaa aggaacaaag agatgttaga
     7861 agcctatcgt aaagccgccg ccgagcgcgc cgccctcggc attcccgccc tccctttgaa
     7921 cgcgcagcaa accgccgatt tggttgagct gctgaaaagc ccgcccgcag gcgaaggcga
     7981 gttcttggtc gaactgcttg cccaccgtgt tccgcccggt gtggacgatg ccgccaaagt
     8041 caaagcctca ttcctggctg ccgttgccga aggcagcgcg tccagcccgc tgatctcccc
     8101 cgaatatgcg accgaactct taggtacaat gctcggcggt tacaatattc acgccttaat
     8161 cgaactcttg gacgacgaca aactcgcgtc cattgctgcc aaaggcttga aacatacgct
     8221 tctgatgttc gattccttcc acgacgttca agaaaaagcc gaaaaaggca acaaatacgc
     8281 gcaagaagtt ttgcaatctt gggcagatgc cgaatggttc gcctcacgcg ccaaagttcc
     8341 cgaaaaaatc accgttaccg ttttcaaagt tgacggcgaa accaatacag acgacctctc
     8401 ccccgcgccc gacgcgtgga gtcgtcccga tattccgctg cacgcgctgg ccatgctgaa
     8461 aaacccgcgc gacggcatca cgcccgacaa accgggcgaa gtcggtccga ttaaattgtt
     8521 ggaagaactc aaagccaaag gccatccggt tgcttacgtc ggcgacgtgg tcggtactgg
     8581 ttcttcacgc aaatccgcga ccaactccgt catttggcat accggcgaag acattccgtt
     8641 cgtgccgaac aaacgcttcg gcggcgtatg tttgggcggc aaaatcgcgc cgattttctt
     8701 caatacccaa gaagattccg gcgcgctgcc gattgaagtc gatgtatctg ctctaaaaat
     8761 gggcgatgtc gtcgatatcc tgccttatga aggcaaaatc gtgaaaaacg gcgagactgt
     8821 tgccgagttt gaattgaaat cacaagtatt gctggacgaa gtgcaagccg gcggccgtat
     8881 caacctgatt atcggccgag gtctgaccgc caaagcgcgc gaagccctga aactgcctgc
     8941 ctctactgca ttccgcctgc cgcaagcgcc tgccgaaagc aaagccggtt tcaccttggc
     9001 gcaaaaaatg gtcggccgcg cctgcggtct gcccgaagga caaggcgtgc gcccgggtac
     9061 ttactgcgaa ccgcgtatga cgacggtcgg ctcgcaagac acgaccggcc cgatgacccg
     9121 cgacgagttg aaagacttgg cttgtttggg cttctccgcc gatatggtga tgcagtcttt
     9181 ctgccacacc gccgcctatc cgaaacctgt cgatgtaaaa acccataaag aactgcccgc
     9241 ctttatttcc acccgtggcg gcgtgtcact gcgtccgggc gacggcgtca tccactcgtg
     9301 gctcaaccgc ctgctgctgc ccgataccgt cggcaccggc ggcgacagcc atacccgttt
     9361 ccccatcggt atttccttcc ccgccggctc cggcttggtt gcctttgccg ccgcaacggg
     9421 cgtaatgccg ctcgatatgc ccgagtctgt attggtacgc ttcagcggca agctgcaacc
     9481 gggcgtaacc ctgcgcgatt tggtgaacgc catcccgctg tacgcaatca aacaaggttt
     9541 gctgaccgtt gccaaagccg gtaagaaaaa catcttctcc ggccgcatcc tcgaaatcga
     9601 aggcctgcct gatttgaaag tggaacaagc ctttgaattg accgacgcat ccgccgaacg
     9661 ctccgccgcc ggctgtaccg tgaagctcaa caaagagccg attatcgagt acatgaaatc
     9721 caacgtcgtg ttgatgaaaa acatgattgc caacggctat caagacccgc gcactttgga
     9781 acgccgcatc aaagctatgg aaaaatggct ggcaaatccc gagttgctcg aagcggataa
     9841 agatgccgaa tacgccgccg tgattgaaat caacatggac gacatcaaag agccgattat
     9901 cgcctgcccg aacgacccgg acgacgtgtg cttcatgtcc gaacgctccg gcaccaaaat
     9961 cgacgaagta ttcatcggtt cgtgtatgac caacatcggc cacttccgcg ccgcctccaa
    10021 acttttggaa ggcaaggcag acacccccgt ccgcctgtgg attgcgccgc cgaccaaaat
    10081 ggacgcgaaa caattgtccg acgaaggaca ctacggcgta ctcggacgtg ccggcgcgcg
    10141 tatggaaatg ccgggttgct ccttatgtat gggtaatcag gcgcaagtac gcgaaggtgc
    10201 gaccgttatg tccacctcca cccgcaactt cccgaaccgt ttgggtaaaa acacctttgt
    10261 ttacctcggt tcggcggaat tggcagcgat ttgctccaaa ctgggtaaaa tcccgaccgt
    10321 tgaagaatat caagccaata tcggcatcat caacgaacag ggcgataaaa tctaccgcta
    10381 tatgaacttc aacgaaatcg acagctacaa cgaagtagcc gagaccgtga acgtttaatc
    10441 cccgtcatcc gtatgaagta agggattgac cgcaatgccg tctgaacaac cttcagacgg
    10501 cattgcaaca ttccgctaac ccttctttcc gcaaacgctg caaatacggc gttcacgccc
    10561 ccacataaag gaaacgacag tgaacctgaa aaaccgccat tttctgaaac ttttagactt
    10621 cacgccggaa gaaatcaccg cctacctcga ccttgccgcc gaattgaaag ccgccaaaaa
    10681 agcagggcgc gagattcagc ggatgaaagg gaaaaacatc gccctgattt ttgaaaaaac
    10741 ctctactcgg acgcgctgcg cgtttgaagt cgccgcgcgc gatcaaggcg cgggagtgac
    10801 ttatttagag ccgtccgcca gccaaatcgg gcataaggaa agcatcaaag acaccgcccg
    10861 cgtgttgggc aggatgtacg atgccatcga atatcgcggt ttcggtcagg aagttgttga
    10921 agaattggcg aaatacgcgg gcgtacccgt gttcaacggg ctgaccaacg agttccatcc
    10981 cacacaaatg cttgccgacg cactgactat gcgcgaacac agcggcaaac ctttgaacca
    11041 aaccgcgttt gcctacgtcg gcgacgcgcg ttacaacatg ggcaattccc tgctgatttt
    11101 aggggcaaaa ttggggatgg acgtgcgtat cggcgcaccg caaagcctgt ggccgtctga
    11161 aggcattatt gccgccgcac acgccgccgc caaagaaacc ggcgcaaaaa ttaccctgac
    11221 cgaaaacgcg catgaagccg tgaagaatgt tgattttatt cataccgatg tgtgggtcag
    11281 catgggcgag ccgaaagaag tctggcagga acgcatcgat ttgctgaaag attaccgcgt
    11341 tacgcccgaa ctgatggcgg catcgggcaa tccgcaagtc aaattcatgc actgcctgcc
    11401 cgccttccac aaccgcgaaa ccaaagtcgg cgaatggatt tacgaaacct tcgggctgaa
    11461 cggtgtggaa gttacagaag aaatattcga aagccccgcc agcatcgtgt tcgatcaggc
    11521 ggaaaaccgt atgcacacga ttaaagcggt aatggtcgcg gctctgggcg actgacagaa
    11581 ctgtgcctgt ttaaattcat ccgcaacaca gataccgtct gaacacgatg ttcagacggt
    11641 atcc
//
LOCUS       DAWISC010000047        11518 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWISC010000047 DAWISC010000000
VERSION     DAWISC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.047"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            204..1046
                     /locus_tag="WIW87_000916"
     CDS             204..1046
                     /locus_tag="WIW87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ5174096.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVAV"
     gene            1076..1540
                     /gene="nrdR"
                     /locus_tag="WIW87_000917"
     CDS             1076..1540
                     /gene="nrdR"
                     /locus_tag="WIW87_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ5174097.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1572..2681
                     /gene="ribD"
                     /locus_tag="WIW87_000918"
     CDS             1572..2681
                     /gene="ribD"
                     /locus_tag="WIW87_000918"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ5174098.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHNIKTVFRKNGNAF"
     gene            2876..4297
                     /locus_tag="WIW87_000919"
     CDS             2876..4297
                     /locus_tag="WIW87_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ5174099.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLAEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLTSSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4465..5706
                     /locus_tag="WIW87_000920"
     CDS             4465..5706
                     /locus_tag="WIW87_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ5174100.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPETSTA"
     gene            5839..7014
                     /locus_tag="WIW87_000921"
     CDS             5839..7014
                     /locus_tag="WIW87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ5174101.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFSSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVGELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLTNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7062..8972
                     /gene="pglD"
                     /locus_tag="WIW87_000922"
     CDS             7062..8972
                     /gene="pglD"
                     /locus_tag="WIW87_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ5174102.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYHAVTRFVSFRILTTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            9028..10320
                     /locus_tag="WIW87_000923"
     CDS             9028..10320
                     /locus_tag="WIW87_000923"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ5174103.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDQAFADIFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQMLEKGIAAIGKTVRKLYDNV"
     gene            complement(10371..11207)
                     /locus_tag="WIW87_000924"
     CDS             complement(10371..11207)
                     /locus_tag="WIW87_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ5174104.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRKGRLKRLE
                     AIAEKLEKKGIGGAYEGALALAANKEMVSRTHIAEFLIKNGYVKNKQQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGLLASAGSDFHRLNDFGGGILGACPELPENCKLVWEHFS
                     RH"
BASE COUNT         2570 a         3678 c         2937 g         2333 t
ORIGIN      
        1 aaaagttacg gaacgaaaaa caaccaaaac cggacaagtc ggattcccgc ctgcgcggga
       61 atgacggaat cttaagtttc cgtctttgtt ttctgttttc tgttttcgag ggaataatgg
      121 ggaacaagcc gtatttcaga cggcattttc agttcggggt ataatccgaa tacttgcgac
      181 catctgaatc attgggacaa accatgtgtc aactgctggg catgaactgc aatacgccga
      241 ccgatattat gttttccttt gaaggcttcc gccgcagggg cggcattacc gaccaccatg
      301 ccgacggttt cggtatcggc tttttcgaag gcaaaggcgt gcgcctgttc cacgacgaca
      361 agccgagcgc aaattccccc gtcgccgacc tcgtgcgtgc ctaccaaatc aaatcggaaa
      421 acgtcatcgc acatatccgc aaagcatcgc aaggacaaac ctcgctggcg aacacccatc
      481 cctttatgcg tgaaatgtgg ggcggctact ggctgtttgc ccacaacgga catttgattg
      541 attttttccc cgaacagggc gaatttttcc accccgtcgg cacaaccgat tccgaacgcg
      601 cgttctgcca catcctcaac cgcctgcgca cccgctttgc cgcccgtccc gacgacgaca
      661 cgctgtttga cgcgattgcg gggctgacgc acgaaatccg caagttcggg ctgtttaact
      721 ttatgctttc agacggcatt gccctgtttg cccacgccag cacgctgctg cactacatcg
      781 tccgccaagc cccgttcggc aaggcgcgcc tgctcgacga cgacgtgatg gtcgattttg
      841 ccgaagtaac cacgccctcc gaccgcgtcg ccgttatcgc caccctgcca ctgacccgcg
      901 acgaatcatg gtcccaactt gccgtggacg aactggtcat gttccgcgaa ggcaacatcg
      961 tccgacacga ccgtcccgaa aaccccgtct atatgagtgc cgaagaaggt ctggaaatcg
     1021 cccgcgccgc cggcgtcgcc gtctgaactt cagacgacat aggaggacga acccgatgaa
     1081 atgcccgttt tgcgcccacc ccgacacccg cgttgccgat tcgcgtctga tggaagaacg
     1141 caacgccgtg cgccgccgcc gccactgccc caactgcggc aaacgcttcg gcacgctcga
     1201 aaccgccgaa ctcaaaatgc ccgccgtcat cggtccggac aaaaaacgct cgccctttaa
     1261 tgcacaacgc ctccgcaacg acctgaccgc cgccgcccga aaatccgccc tgacacccga
     1321 acagatcgac gaaaccgtcc gcctgacgga acacaggctc tacacttcgg gtcagcgcga
     1381 catcccctct gccgcacttg ccgacatggt gctaaaagaa ctgctccgac aagatacgga
     1441 agccgccgtc cgtttcgccg ccctgcacaa acgcttcgac aatccggcag actttgcctc
     1501 gtggctggcg caagccgtca aaacaggcgg caaagcctga ttcccccaac ccatactgat
     1561 acggtatccc tatgttttcg gacacagata tatccatgat ggaaaacgcc ctccgacttg
     1621 ccgctttggg gcgtttttcc acttcgccca atccgcgcgt cggctgcgtt atcgcacacg
     1681 gcagccaaat tgtcgggcaa ggcttccacg tcaaagcggg cgaaccccat gccgaagtcc
     1741 acgccctgcg tcaggcgggc gaaatggcac aaggcgcgac cgcctttgtt accctcgaac
     1801 cgtgcagcca ttacgggcgc acaccgccct gtgccgaagc actggtgcgg gcgggcgtgt
     1861 cccgcgtcgt tgccgccatg cgcgacccca acccgctggt tgcaggcaaa gggcttgccc
     1921 tgctcgaagc agcaggcatc aagacggaat gcggtttact cgaacatcag gcaagggaac
     1981 tcaaccgagg cttcctgtcg cgcatcgaac gccgccgccc ctttgtccgc ctcaaatgcg
     2041 ccgtttcgct ggacggcaaa accgcccttt cagacggcag cagcttttgg attaccggcg
     2101 aagacgcgcg tgccgacgta caggttttgc gtgccgaaag ctgcgcggtg ctgaccggca
     2161 tcggcacggt gttggcggac aatccccggc tcaacgtccg cgcttttcca actttgcgcc
     2221 aacccgcacg catcgtttta gacagccgcc tgcgcctgcc cccgaacagc catttggtta
     2281 ccgacggaca atctccgacc tacatcgcca cactcgaacg caacgaagac agactgcacc
     2341 cctatcggga acacgcacac gtccgcatcc tgatgccgtc tgaaacggca gacagcaaaa
     2401 tcgacctgca ccacctgatg cgcctccttg ctgacgaagg tttcggcgaa atcatggtcg
     2461 aagcaggctc cgaactcaca tccgcatttt tggcagaaaa tctggcagac gaaatcgtcc
     2521 tgtaccgttc gcccaaaatc ctcggcagcg gcaaagacct gttttccctg ctcgaaaacc
     2581 gcgccgccct ttccgcaccg cccttgtgga cacccgtttc aagcgaaatc ctcggacaca
     2641 acatcaaaac cgtgttccga aaaaacggca acgcctttta aagggtttgc gccgtttcac
     2701 tatataataa cgccgataaa aaacggcccc gttcagaccg ccgcccgccc gaaaaaacgc
     2761 aacccggact gccgcacccc gccggcagcc gcgacggtct gaaagccgtc aaattccgat
     2821 caagaaaggc ttcagacggc acaggcagca tcccgccgcc gcccggacat caaaaatgga
     2881 cacaaaagaa atcctcggct acgcggcagg ctcgatcggc agcgcggttt tagccgtcat
     2941 catcctgccg ctgctgtcgt ggtatttccc cgccgacgac atcgggcgca tcgtgctgat
     3001 gcagacggcg gcggggctga cggtgtcggt gttgtgcctc gggctggatc aggcatacgt
     3061 ccgcgaatac tatgccaccg ccgacaaaga caccttgttc aaaaccctgt tcctgccgcc
     3121 gctgctgtct gccgccgcga tagccgccct gctgctttcc cgcccgtccc tgccgtctga
     3181 aatcctgttt tcactcgacg atgccgccgc cggcatcggg ctggtgctgt ttgaactgag
     3241 cttcctgccc atccgctttc tcttactggt tttgcgtatg gaaggacgcg cccttgcctt
     3301 ttcgtccgcg caactcgtgc ccaagctcgc catcctgctg ctgctgccgc tgacggtcgg
     3361 gctgctgcac tttccagcga acaccgccgt cctgaccgcc gtttacgcgc tggcaaacct
     3421 tgccgccgcc gcctttttgc tgtttcaaaa ccgatgccgt ctgaaggccg tccggcacgc
     3481 accgttttcg cccgccgtcc tgcaccgggg gctgcgctac ggcataccga tcgcactgag
     3541 cagcatcgcc tattgggggc tggcatccgc cgaccgtttg ttcctgaaaa aatatgccgg
     3601 cctggaacag ctcggcgttt attcgatggg tatttcgttc ggcggggcgg cattattgtt
     3661 ccaaagcatc ttttcaacgg tctggacacc gtatattttc cgcgcaatcg aagaaaacgc
     3721 cccgcccgcc cgcctctcgg caacggcaga atccgccgcc gccctgcttg cctccgccct
     3781 ctgcctgacc ggcattttct cgccccttgc ctccctcctg ctgccggaaa actacgccgc
     3841 cgtccggttt atcgtcgtat cgtgtatgct gccgccgctg ttttgcacgc tggcggaaat
     3901 cagcggcatc ggtttgaacg tcgtccgcaa aacgcgcccg atcgcgctcg ccaccttggg
     3961 cgcgctggcg gcaaacctgc tgctgctggg gcttgccgtg ccgtccggcg gcgcgcgcgg
     4021 cgcggcggtt gcctgtgccg cctcattctg gctgtttttt gccttcaaga ccgaaagctc
     4081 ctgccgcctg tggcagccgc tcaaacgcct gccgctttat ctgcacacat tgttctgcct
     4141 gacctcctcg gcggcctaca cctgcttcgg cacgccggca aactatcccc tgtttgccgg
     4201 cgtatgggcg gcatatctgg caggctgcat cctgcgccac cggaaagatt tgcacaaact
     4261 gtttcattat ttgaaaaaac aaggtttccc attatgaaaa tcgttttgac cacatctatg
     4321 gcaggcttgg gcggcacggg cacgatatca tcgattgcca aatggcgtgc aaaaacgacc
     4381 ccgttcagtc cgacgaaatc gtccgccgct tcaggcgcga catttcctat cggaaaatcg
     4441 tcaacctgat tgaaagattg gcaaatgagt aaattcttca aacgcctgtt tgacattgtt
     4501 gcctccgcct cgggactgat tttcctctcg ccagtatttt tgattttgat atacctcatc
     4561 cgcaagaatc taggttcgcc cgtcttcttc tttcaggaac gccccggaaa ggacggaaaa
     4621 ccttttaaaa tggtcaaatt ccgttccatg cgcgacgcgc ttgattcaga cggcattccg
     4681 ctgcccgacg gagaacgcct gacaccgttc ggcaaaaaac tgcgtgccgc cagtttggac
     4741 gaactgcctg aattatggaa tatcttaaaa ggcgagatga gcctggtcgg cccccgcccg
     4801 ctgctgatgc aatatctgcc gctgtacgac aacttccaaa accgccgcca cgaaatgaaa
     4861 cccggcatta ccggctgggc gcaggtcaac gggcgcaacg cgctttcgtg ggacgaaaaa
     4921 ttcgcctgcg atgtttggta tatcgaccac ttcagcctgt gcctcgacat caaaatccta
     4981 ctgctgacgg ttaaaaaagt attaatcaag gaagggattt ccgcacaggg cgaagccacc
     5041 atgccccctt tcacaggaaa acgcaaactc gccgtcgtcg gtgcgggcgg acacggaaaa
     5101 gtcgttgccg accttgccgc cgcactcggc cggtacaggg aaatcgtttt tctggacgac
     5161 cgcgcacaag gcagtgtcaa cggcttttcc gtcatcggca cgacgctgct gcttgaaaac
     5221 agtttatcgc ccgaacaata cgacgtcgcc gtcgccgtcg gcaacaaccg catccgccgc
     5281 caaatcgccg aaaaagccgc cgcgctcggc ttcgccctgc ccgttctggt tcatccggac
     5341 gcgaccgtct cgccttctgc aacagtcgga caaggcagcg tcgttatggc gaaagccgtc
     5401 gtacaggcag gcagcgtatt gaaagacggc gtgattgtga acactgccgc caccgtcgat
     5461 cacgactgcc tgcttaacgc tttcgtccac atcagcccag gcgcgcacct gtcgggcaac
     5521 acgcatatcg gcgaagaaag ctggataggc acgggcgcgt gcagccgcca gcagatccgt
     5581 atcggcagcc gcgcaaccat tggagcgggc gcagtcgtcg tacgcgacgt ttcagacggc
     5641 atgaccgtcg cgggcaatcc ggcaaagccg ctgccgcgca aaaaccccga gacctcgaca
     5701 gcataagcga ttaaaataca cccccgtaca gaccgatttt gacaacacct gcggcgcgcg
     5761 cccgattctt cggaacacgc cccccttcag acggcatagg gtcggaaatg ccgtctgaaa
     5821 accgacggac aaaccatcat gctgaacact ttcctttccc cgtggccctg cttcacccaa
     5881 gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta ctggacgggc
     5941 aacgaatgcc gcgaatttga aaaagaattt gccgcctttg ccggcacgcg gtacgccgtc
     6001 gcccttgcca acggcacgct ggcactcgat gtcgcgctca aagcaatggg cataggcgcg
     6061 ggcgacgatg tgattgttac ctcgcgcacc ttcctcgctt ccgcgtcctg cattgtgaac
     6121 gcgggcgcaa accccgtgtt tgccgatgtg gatttgaaca gccaaaacat cagcgcggaa
     6181 accgtcaaag ccgcgctgac accgactacc aaagccgtca tcgtcgtcca cctcgccggt
     6241 atgcccgccg aaatggacgg cattatggct ttggcaaaag aacataatct ttgggtaatc
     6301 gaagactgcg cccaagcgca cggcgcaaaa tacaaaggca aatccgtcgg ctctatcgga
     6361 cacgtcggcg cgtggtcgtt ctgccaagac aaaatcatga ccaccggcgg cgaaggcggt
     6421 atggttacga ccaacgacaa aaccctgtgg gaaaaaatgt ggtcgtacaa agaccacggc
     6481 aaaagctacg atgccgtgta caaccacgaa cacgcgcccg gtttccgctg gctgcacgaa
     6541 agtttcggca caaactggcg tatgatggaa atgcaggcgg tcatcggacg catccagctc
     6601 aaacgcctgc ccgaatggac ggcgcgccgc cgagaaaacg ccgccaagct ggcggaaagt
     6661 ttgggcaaat tcagcagcat ccgcttggtt gaagtcgccg actacatcgg acacgcgcaa
     6721 tataagttct acgccttcgt caaacccgaa cacctcaaag acggctggac gcgcgaccgc
     6781 atcgtcggcg aactgaacgc gcgcaaagtc ccctgctatc aaggcagctg ctccgaagtc
     6841 tatttggaaa aagccttcga caacacgccg tggcgaccga aagagcgttt gacaaatgct
     6901 gtcgagttgg gcgacaccag cctgatgttc ttggtgcacc cgacgctgac cgacgacgaa
     6961 attgcgtttt gcaaaaaaca catcgaagcc gtcttgaccg aagccgcacg ataacccttc
     7021 agacggcata tgccgcctga aaacacatac cgccccacga tatgaatctg gaaactctga
     7081 tcgccctgcc gcgcaacatc aagaaaatct gtttcctcat acacgatttt ctgatgattt
     7141 tcattgcctt ttggttcacc caaagcctaa aggccgacta ctcggacgaa tggttcgatt
     7201 ttgccaactg gcagtctttt ttgctgactg ccttgctgac catcacatta tttatccgaa
     7261 tggggcttta ccacgccgtt acacgcttcg tcagcttccg catcctcacc accgcactgg
     7321 cgggcagcct cgcctccgcc gtgttgtttt tcctcaatac gctgatattt gaagaaaggc
     7381 tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg accggctcgc
     7441 gtatggtttt gcgcggactg ttgtccgaac accccaaaaa acagatgatc cctgtcatca
     7501 tttacggcgc gggacggtcg ggcagacaac tgcttgaggc cgtcaaacaa atgcgcgaat
     7561 attccgccgc cgcctttgta gacgacgacc ccaaactgtg gcacaccgtc atctacgacc
     7621 ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc gtggaaaaaa
     7681 tcctgctcgc catccccggc gcgacccagg aacaacgccg ccgaatcatc aacaaactgg
     7741 aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg gacggaaaaa
     7801 tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgt gattccgtcg
     7861 cgcccgacga ccgcctgatg agtgccgaca tcgaaggcaa aaccgtcatg gtaaccggcg
     7921 cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc cccgaaaagc
     7981 tgctgctgtt cgagttatcc gaattcgccc tgtacgccat cgaaaaagaa ttgcgcgaaa
     8041 cctgcatcca aaaacgcctc gacaccgaaa tcctgccctt tctcggttcg gtgcaaaacc
     8101 gcacgctgct cgaacacgtc atgaccgcct tttccgttgc gaccgtctat cacgccgctg
     8161 cctacaaaca cgtccccatg gtcgagttca acaccgtcga aggcatacgc aacaacatct
     8221 tcggcacact cgagtgcgcg cttgccgcca cgacatcggg cgtaagaact ttcgtcctca
     8281 tctccaccga caaagccgtc cgccccacca acaccatggg tgccagcaaa cgcatggcgg
     8341 aactctgcct tcaggcactc gccgccgaac ccggacaaaa aacccgcttc agcatggtac
     8401 gtttcggcaa tgttttaggt tcgtccggct ccgttgtccc gctgtttgaa aaacagattg
     8461 cagaaggcgg cccgcttacc ctgacccacc ccgaaatcac acgttatttc atgaccatac
     8521 ccgaagccgc ccaactcgtc atacaggcag gcgcgatggg tacgggcggc gacgtattcg
     8581 tcctcgacat gggtgaatcc gtcaaaatca tcgaccttgc ccgccaaatg attaccctaa
     8641 gcggcctcaa acccaacaca cccgaacaac ccgacggcga catcgaaatc ctcattaccg
     8701 gactgcgtcc cggagaaaaa ctctacgaag agctgctcat cggcgacaac gtccgcaaaa
     8761 ccggccatcc gcgcatcatg accgccaacg agaccatgct gccgtggcac gagctctccg
     8821 ccctgctcga ccgcatccgt gcggcctgcg accgttacga ccagcaggca atccgcaccc
     8881 tgctcatcaa cgccccgacc ggctttgccc cgagcgacgg catctgcgac ctgctttggg
     8941 tacgagaaac acacagaaaa aatgccgtct gaaccttcag acggcataac gtacaaacca
     9001 acctacctta cacacacgga gtttgacatg cagttctcag cattcggcga aaaattcacg
     9061 caacacagcg gcatcctcca actgatggac gacctcggcg acgcgctcaa aagcgacaag
     9121 cccgtcaaca tgctcggcgg cggcaacccg gcgcgcattc cggaaatcga tcaggcgttc
     9181 gccgacatat tctccaaact ggcggcagaa cacgccgtcg aaaacatcgg caactactcc
     9241 aatccccaag gcgatgccgt gctgattgac gcgctgaccg ccttcctcaa ccgcgaatac
     9301 agctggaatc tgaccgccga caatatcgcg ctgaccaacg gttcgcaaaa cgcgtttttc
     9361 tatcttttca acctcttcgg cggcaaattc aacctttcag acggcacatc cgcagaaaaa
     9421 gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca tgtcgaaggg
     9481 cagcacttcg tttccgtcaa gcccaaaatc gaaaacgtcg aacacgaagg cgaagccggc
     9541 ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact caaagcgggc
     9601 aaaatcggcg cgatttgctg ttcgcgcccg accaacccga ccggcaatgt gttgaccgac
     9661 ggcgaaatgg cgcgtttgga cgctttggcg cgtgaacacg gcattccgct gattatcgac
     9721 aacgcctacg gaatgccgtt ccccaacatc atttacagcg acgtaacgct gaattggcac
     9781 gaaaacatca tcctctgctt cagcctgtcc aaagtcggcc tgccgggcgt gcgcaccggc
     9841 atcatcgtcg ccgcgcccga agtcgtcaaa gccgtcagca gcctgaacgc gattgtgaac
     9901 cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tactggaaag cggcgagatg
     9961 aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca aaccgccgtc
    10021 tcgctgctca agcgggagct gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg
    10081 attttcctgt ggctctggtt tgaaaacctg cccgtttctt cgcaaaccct gtacgaaatg
    10141 ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg catcgacacg
    10201 caggattacc cgcacgcggg cgagtgcatc cgcatgagca tcgcgcagga cgctcaaatg
    10261 ctggaaaaag gcattgccgc catcggcaaa accgtccgaa aactgtacga caacgtttaa
    10321 aacgcaaaaa tgccgtctga aaggttttca gacggcattt ttatccgcat tcaatgtcgg
    10381 gaaaaatgtt cccacaccag tttacagttt tccggcaatt cgggacatgc gccgaggatg
    10441 ccgccgccga agtcgtttaa acggtggaag tcgctgcccg cgcttgccaa caatccgaag
    10501 cgttctgcca aaaatgcgta gttgaggcgg tcgtttttgc agcagttgcc gctgtggact
    10561 tcgatgcccg cgccgccgag gtttttaaat tcttcaaaca gattgcgctt ggcggtggcg
    10621 gacaaatcat aacgcatcgg atgggcgatg actgccatgc cgcccgcacc gttgacggcg
    10681 gagacgcagt ctgccagcgt cgcccattcg tgtcggacag cgcaggattt gccgtcgccc
    10741 aagtatttgg tgaacgcctg ctgcttgttt ttgacgtatc cgtttttgat gaggaactcg
    10801 gcgatgtggg tgcggctgac catttctttg tttgccgcca gcgcgagcgc accttcgtat
    10861 gcgccgccga tgcctttctt ttcaagtttt tcggcgatgg cttcaagacg tttcagacga
    10921 cctttccgca cttgcgccaa caggttttgc aggttttcgt cctgctcgtc gaaatccaaa
    10981 ccgacaacgt gtatggtgcg cccgcgccac gttacggaga tttccacacc gttaatcagg
    11041 cgcaaaccga gcctgtcggc ttcggcacgc gcttcggcga tgccgccggt gtggtcgtga
    11101 tcggtcaacg ccagcagcgt gcagccgttt tgatgcgcga ggcgcacgac ttcggcgggg
    11161 gagagcatac cgtcggaaac ggtggaatgg cagtgcaggt ctatcatggg gtgttatgtg
    11221 tgttgtgaat gaagctcggg atgtttcaat gttgtcgggc agtgtggcgg cggcggtcat
    11281 tttaaaaaac aggtgtatgg caaatgcaaa gaaaaagtcg ctataccgtc attcccgcgc
    11341 aggcgggaat ccagactttg atttgtcaaa aatgtttaag gttaaccgct atttcgaact
    11401 tccggattcc cgcctgcgcg ggaatgacga tatggacgtt ttcaatttta atctactata
    11461 aaagactgtc tgaaaacgtg gttttatagt gaattaaatt taaaccggta cagcgttg
//
LOCUS       DAWISC010000048        11400 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWISC010000048 DAWISC010000000
VERSION     DAWISC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.048"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..79)
                     /locus_tag="WIW87_000925"
     CDS             complement(<1..79)
                     /locus_tag="WIW87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ5174105.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WIW87_000926"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WIW87_000926"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ5174106.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WIW87_000927"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WIW87_000927"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ5174107.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4598)
                     /gene="xerC"
                     /locus_tag="WIW87_000928"
     CDS             complement(3693..4598)
                     /gene="xerC"
                     /locus_tag="WIW87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ5174108.1"
                     /translation="MGLDGLAAYLDAYLENIVREGKSEHTVAAYRRDLQELLALLEEM
                     PSANPSNCTRGDFVQALRRLSGRGLGERTLARKLSSWRQYCVWLVKRGLMHADPTADI
                     KPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIHGLNA
                     DDVYLDEAWVHVIGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNGTRLS
                     QRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLSTTQI
                     YTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4782..5846
                     /locus_tag="WIW87_000929"
     CDS             4782..5846
                     /locus_tag="WIW87_000929"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ5174109.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVDAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVCFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5866..6828
                     /locus_tag="WIW87_000930"
     CDS             5866..6828
                     /locus_tag="WIW87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HEZ5174110.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTTALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGS
                     VLYNQAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQ"
     gene            6975..7652
                     /gene="tsaB"
                     /locus_tag="WIW87_000931"
     CDS             6975..7652
                     /gene="tsaB"
                     /locus_tag="WIW87_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ5174111.1"
                     /translation="MQADFNRPVLAVDTGTSRLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFRDAGITAADLGAVVYAQGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAATDARMGEVFYAWFDTLNCHRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEEAPPFSGRPDMPTAADFLALAAKGGYPAVHAAHAGLLYVRNKIALTAKEQ
                     AERRARP"
     gene            7649..8089
                     /gene="rimI"
                     /locus_tag="WIW87_000932"
     CDS             7649..8089
                     /gene="rimI"
                     /locus_tag="WIW87_000932"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ5174112.1"
                     /translation="MNIRRAVCADCEELAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFTHLPEDTQRLLLE
                     VRAGNTAAQALYTAHGFSITGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8083..8820
                     /locus_tag="WIW87_000933"
     CDS             8083..8820
                     /locus_tag="WIW87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ5174113.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8882..9523
                     /gene="pyrE"
                     /locus_tag="WIW87_000934"
     CDS             8882..9523
                     /gene="pyrE"
                     /locus_tag="WIW87_000934"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ5174114.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9597..10031
                     /locus_tag="WIW87_000935"
     CDS             9597..10031
                     /locus_tag="WIW87_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174115.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10028..11338
                     /gene="argA"
                     /locus_tag="WIW87_000936"
     CDS             10028..11338
                     /gene="argA"
                     /locus_tag="WIW87_000936"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ5174116.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2552 a         3570 c         2994 g         2284 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgacc gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aactggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcaa tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gtcctgattt gtgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gtacaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcagc
     2761 aactgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccata ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gtgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aataggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 ataacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggcgtgcatc
     4321 aggccgcgct ttaccagcca aacgcaatac tgccgccaag acgaaagctt gcgagccagc
     4381 gtccgttccc ccaaaccgcg gccggacagc cggcgtaatg cctgtacgaa gtcgccgcga
     4441 gtgcaatttg aagggtttgc agacggcatt tcttccagaa gggcaagcag ttcctgcaag
     4501 tcgcgccggt atgcggcaac cgtgtgctcc gatttaccct cgcgcacgat attttccaaa
     4561 taagcgtcca agtatgccgc aagtccgtcc aaacccattc ccacacctaa aataacatta
     4621 gaaacattat cataaatcgg aatatccgaa tcccgaaacg tcaaaacccg acaaacctgc
     4681 atactggcat cattaatata aaatcaatga gctgtttatg gttttttgct gtaaaaaaca
     4741 ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc gtatccatgc
     4801 gccaactgct tgatcatgct gccgaaaaca gctacggcct gccggcgttc aacgtcaaca
     4861 acctcgaaca gatgcgcgcc atcatggagg ctgcagacca agtcgacgcc cccgtcatcg
     4921 tacaggcgag tgccggtgcg cgcaaatatg cgggtgcgcc gtttttacgc cacctgattt
     4981 tggcggctgt cgaagaattt ccacacatcc ccgtcgtcat gcaccaagac cacggcgcat
     5041 cacccgacgt gtgccaacgc tccatccaac tgggcttctc ctctgtaatg atggacggct
     5101 cgctgatgga agacggcaaa accccttctt cttacgaata caacgtcaac gccacacgta
     5161 ccgtggttaa cttctcccac gcttgcggcg tatccgttga aggcgaaatc ggcgtattgg
     5221 gcaacctcga aaccggcgaa gcaggcgaag aagacggtgt aggcgcagtg ggcaaacttt
     5281 cccacgacca aatgctgacc agcgtcgaag atgccgtatg tttcgttaaa gataccggcg
     5341 ttgacgcatt ggctattgcc gtcggcacca gccacggcgc atacaaattc acccgtccgc
     5401 ccacaggcga tgtattacgt atcgaccgca tcaaagaaat ccaccaagcc ctgcccaata
     5461 cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa gtcatcaacg
     5521 aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc gtcgaaggca
     5581 tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgactt gcgccttgct tctaccggcg
     5641 cggtacgccg ctaccttgcc gaaaatccgt ccgactttga cccgcgcaaa tacctgagca
     5701 aaaccattga ggccatgaag caaatctgcc tcgaccgtta tcttgcgttt ggctgcgaag
     5761 gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt tatgccaagg
     5821 gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa acaaaatgcc gtctgaaccg
     5881 ccgttcggac gacatttgat ttttgcttct ttgacctgcc tcattgatgc ggtatgcaaa
     5941 aaaagatacc ataaccaaaa tgtttatata ttatctattc tgcgtatgac taggagtaaa
     6001 cctgtgaatc gaactgcctt ctgctgcctt tctctgacca ctgccctgat tctgaccgcc
     6061 tgcagcagcg gagggggtgg tgtcgccgcc gacatcggtg cggggcttgc cgatgcacta
     6121 accgcaccgc tcgaccataa agacaaaggt ttgcagtctt tgacgctgga tcagtccgtc
     6181 aggaaaaacg agaaactgaa gctggcggca caaggtgcgg aaaaaactta tggaaacggt
     6241 gacagcctca atacgggcaa attgaagaac gacaaggtca gccgtttcga ctttatccgc
     6301 caaatcgaag tggacgggca gctcattacc ttggagagtg gagagttcca agtatacaaa
     6361 caaagccatt ccgccttaac cgcctttcag accgagcaaa tacaagattc ggagcattcc
     6421 gggaagatgg ttgcgaaacg ccagttcaga atcggcgaca tagcgggcga acatacatct
     6481 tttgacaagc ttcccgaagg cggcagggcg acatatcgcg ggacggcgtt cggttcagac
     6541 gatgccggcg gaaaactgac ctacaccata gatttcgccg ccaagcaggg aaacggcaaa
     6601 atcgaacatt tgaaatcgcc agaactcaat gtcgacctgg ccgccgccga tatcaagccg
     6661 gatggaaaac gccatgccgt catcagcggt tccgtccttt acaaccaagc cgagaaaggc
     6721 agttactccc tcggtatctt tggcggaaaa gcccaggaag ttgccggcag cgcggaagtg
     6781 aaaaccgtaa acggcatacg ccatatcggc cttgccgcca agcaataacc attgtgaaaa
     6841 tgccgtccga acacgataat ttaccgttcg gacggcattt tgtattgcac cgtccgacgg
     6901 catgcccaag gggggaaatc cctattttca ggccaaccgc tatataatgc cgtctgaacc
     6961 aacgagagaa tgccatgcaa gctgatttta accgtcccgt cctggccgtc gataccggta
     7021 cttcccgttt gtcgctcgcg ctgcgtgccg acggcgaaac ccgtctgttc catcaggaag
     7081 tcggcagccg ccagtccgaa ctgattctgc cggaaatccg caccctattc cgcgatgcag
     7141 gcattaccgc cgccgatttg ggtgcggtcg tgtacgcaca gggtcccggc gcgtttaccg
     7201 gactgcgtat cggcatcggt gtagctcagg gtttggcaac gccgtttgat acccccttaa
     7261 tcggcgtacc ctcgctcgat gccgccgcct cgctgccgcc gccgcaaagc tgcatccttg
     7321 ccgctacgga cgctcgtatg ggcgaagtgt tttatgcatg gttcgatacg ctgaactgcc
     7381 accgtttgag cgattatcag gtcgggcggg cggcagacat ccggctgccg gagggatgcg
     7441 ccttttcaga cggcataggc agcgcgttcg cgctggaaga agctccgccg ttctcaggca
     7501 gaccggatat gccgactgcc gccgactttc tcgcattggc agccaagggc ggttatcctg
     7561 ccgtccatgc cgcacacgcc ggtttgctct acgtccgcaa caaaatcgcc ctgactgcca
     7621 aagaacaggc cgaacggaga gcgcgcccgt gaacatccgc cgtgccgttt gtgccgattg
     7681 tgaggagctg gccgcactcg atgccgtctg caacccgtcc gcatggacgc aacgccaatt
     7741 tgagtccgca ctggtttcgc cgtccgaaca ggttttcctt gcggaaaaag acggcgggat
     7801 tgccgccttt atcgtttggc agaacctgcc cgacgaatcc gaactgcacc tgattgccac
     7861 cgcgcccgaa tgccgccgcc aaggaattgc gtccgccctg ctcgaatatt ggttcacaca
     7921 tctgcccgaa gacacgcaac gcctgctgct cgaagtccgt gcaggcaaca ccgccgcaca
     7981 ggcactgtac acggcgcacg gcttcagcat tacgggcagg cggaaaaact attaccgtac
     8041 agccgacggt aaaaccgaag atgccgtctt aatggagaaa atatgttaag cgcgcgctac
     8101 ctccacctgc acgaagcttt gggtttgggt ccgatgtggc tgaaacaggc cgccgccgtc
     8161 ctgccgccca aaaacacacc cgcaccctcg gcacaggcac gtccccaaac cgtccgcgcc
     8221 gccccgatcc gcccttccca accccataac ggtcaggcgc ggctcgaaac gatgaaagcg
     8281 ttggaaaccg ccgccgtacc tacgcgcaaa cccgcgcctg aaaccgaaac gcctctgccc
     8341 ggcctttcag acggcatcgc ccccgttccc gccgcttcgg gcatcaccaa gcttgccgtc
     8401 gtcagccttt gcccaccgat cgaggatgcg gtttacgggc aactgttcca cggcaaagca
     8461 ggcatcctgc tcgacaacat actcaaagcc gtaggactgg atgccgccta tgtccacaaa
     8521 acctgttggg tgaaaaccgc cgccgtcggc aacccgatgc cgtctgaaca ggccgtcgcg
     8581 aatgcgctgg gtcaaatcgc ccgcgaactc gacggctgcc gcgccccggc tgtccttttc
     8641 ctcgggcagg cttttgtcca gcctgaacgg caaacgatga ttgaaacttt gtgcggcagc
     8701 cgtcccttct tcatcatcga ccatcccgcc cggcttttac gccaacccga actcaaagcc
     8761 cgcgcctggc aggtgttgaa acagttgaaa cgcgccttgc ggcaaggcgg cggcagttga
     8821 agcgcgccgc acggggcggt agaatcgcaa ctgcgtccca atatctgaca gaaagcacaa
     8881 aatgaccgat ttccgccaag atttcctcaa attctccctc gcccaaaatg ttttgaaatt
     8941 cggcgaattt accaccaagg caggacggcg gtcgccctat ttcttcaatg ccggcctctt
     9001 taacgacggc ttgtccacgc tgcaactggc aaaattttac gcacaatcca tcattgaaag
     9061 cggcatccga ttcgatatgc tgttcggtcc cgcctacaaa ggcattattt tggcggcggc
     9121 aaccgcgatg atgctggcgg aaaaaggcgt gaacgtcccg tttgcctaca accgcaaaga
     9181 agccaaagac cacggcgaag gcggcgtgtt ggtcggcgcg ccgcttaaag ggcgcgtgct
     9241 gattatcgac gacgtgattt ccgccggcac atccgtacgc gaatcgatca aactgattga
     9301 agcggagggt gcaacccccg ccggtgtcgc catcgcgctc gatcgcatgg aaaaaggcac
     9361 gggtgaattg agcgcggttc aggaagtgga aaaacaatac ggtctgcccg tcgcccccat
     9421 cgccagcctg aacgatttgt ttattctgtt gcaaaacaac cccgaattcg gacagttcct
     9481 cgaacccgtc cgagcctacc gtcggcagta cggcgtagaa taaaaacaaa gcatatgccg
     9541 tccgaaccgc cttacgcctc agacggcatc aaacctgaca cacacgagga aataccatgc
     9601 ccgcctgttt ctgcccccac tgcaaaaccc gtctctgggt caaagaaacc caactcaatg
     9661 tcgcccaagg cttcgtcgtc tgccaaaaat gcgaaggact gtttaaagcc aaagaccatc
     9721 tggcaagcac gaaagaaccc atattcaacg atttgcccga ggctgtttcg gatgtcaaac
     9781 tcgttcaccg tatcggcacg cgcgccatcg gcaagaaaca gatttcccgt gacgaaatcg
     9841 ccggcatcct caacggcggt acaacccagc ccgatattcc gcccgcaacc gccgccaccc
     9901 ctgctgccgc accgcaggtt accgtaccgc ccgccgcgcc cgcccgtcag gatgggttca
     9961 actggacgat tgcaaccctg tttgccctta tcgtcctcat tatgcagctt tcctacctcg
    10021 tcatcctatg agcgcgcccg acctctttgt cgcccacttc cgcgaagccg tcccctacat
    10081 ccgccaaatg cgcggcaaaa cgctggtcgc cggcatagac gaccgcctgc tcgaaggtga
    10141 taccttaaac aagctcgccg ccgacatcgg gctgttgtcg caactgggca tcaggctcgt
    10201 cctcatccac ggcgcgcgcc acttcctcga ccgccacgcc gccgctcaag gccgcacgcc
    10261 gcattattgc cggggcttgc gcgttaccga cgaaacctcg ctcgaacagg cgcagcagtt
    10321 tgccggcacc gtccgcagcc gttttgaagc cgcattgtgc ggcagcgttt ccgggttcgc
    10381 gcgcgcgcct tccgtcccgc tcgtatcggg caacttcctg accgcccgtc cgataggtgt
    10441 gattgacgga accgatatgg aatacgcggg cgttatccgc aaaaccgaca ccgccgccct
    10501 ccgtttccaa ctcgacgcgg gcaatatcgt ctggctgccg ccgctcggac attcctacag
    10561 cggcaagacc ttctatctcg atatgcttca aaccgccgcc tccgccgccg tctcgcttca
    10621 ggccgaaaaa ctcgtttacc tgaccctttc agacggcatt tcccgccccg acggcacgct
    10681 cgccgaaacc ctctcggcac aggaagcgca atcgctggcg gaacacgccg gcggcgaaac
    10741 gcgacggctg atttcgtccg ccgttgccgc gctcgaaggc ggcgtgcatc gcgtccaaat
    10801 cctcaacgga gccgccgacg gcagcctgct gcaagaactc ttcacccgca acggcatcgg
    10861 cacgtccatt gccaaagaag ccttcgtctc catccggcag gcgcacagcg gcgacatccc
    10921 gcacatcgcc gccctcatcc gcccgctgga agaacagggc atcctgctgc accgcagccg
    10981 cgaatacctc gaaaaccaca tttccgaatt ttccatcctc gaacacgacg gcaacctgta
    11041 cggttgcgcc gccctgaaaa cctttgccga agccgattgc ggcgaaatcg cctgccttgc
    11101 cgtctcgccg caggcacagg acggcggcta cggcgaacgc ctgcttgccc acattatcga
    11161 taaggcgcgc ggcataggca taagcaggct gttcgcactg tccacaaata ccggcgaatg
    11221 gtttgccgaa cgcggctttc agacggcatc ggaagacgag ttgcccgaaa cgcggcgcaa
    11281 agactaccgc agcaacggac ggaactcgca tattctggta cgtcgcctgc accgctgacc
    11341 gcaacggaaa gccgccgcag aaatgccgtc tgaaccccgt ttcagacggc atttccccga
//
LOCUS       DAWISC010000049        11310 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWISC010000049 DAWISC010000000
VERSION     DAWISC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.049"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            57..338
                     /locus_tag="WIW87_000937"
     CDS             57..338
                     /locus_tag="WIW87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5174117.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(359..1585)
                     /locus_tag="WIW87_000938"
     CDS             complement(359..1585)
                     /locus_tag="WIW87_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ5174118.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1600..2025)
                     /locus_tag="WIW87_000939"
     CDS             complement(1600..2025)
                     /locus_tag="WIW87_000939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174119.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="WIW87_000940"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="WIW87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ5174120.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="WIW87_000941"
     CDS             2817..3335
                     /locus_tag="WIW87_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ5174121.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDNYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="WIW87_000942"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="WIW87_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ5174122.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYGRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="WIW87_000943"
     CDS             4033..4404
                     /locus_tag="WIW87_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ5174123.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKQVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATTLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAMLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="WIW87_000944"
     CDS             4401..5039
                     /locus_tag="WIW87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ5174124.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMGLSDILGRLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5374..5450)
                     /locus_tag="WIW87_000945"
     tRNA            complement(5374..5450)
                     /locus_tag="WIW87_000945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5414..5416),aa:Pro,seq:cgg)
     gene            complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WIW87_000946"
     CDS             complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WIW87_000946"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ5174125.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVARLALAL
                     QKGLTKGGMKSCGKHFPGHGFVEGDSHLVLPEDWRSLSELETADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPTLAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6820..8340)
                     /locus_tag="WIW87_000947"
     CDS             complement(6820..8340)
                     /locus_tag="WIW87_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ5174126.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8542..10041)
                     /locus_tag="WIW87_000948"
     CDS             complement(8542..10041)
                     /locus_tag="WIW87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ5174127.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAENDSDPIADNDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WIW87_000949"
     CDS             complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WIW87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ5174128.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10854..11267)
                     /locus_tag="WIW87_000950"
     CDS             complement(10854..11267)
                     /locus_tag="WIW87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5174129.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFTWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2653 a         3013 c         3159 g         2485 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttaatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gaagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac aggaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 taaagaggga acgcgcggtt tccagacaga acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacacg tttgcctgta taatccgcat ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca acaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaacctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acgtgggcgt
     2641 tggcggacga aatgggcgtg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacaactacc gcgaggccgt ccccgcgctc aaagccgcgt
     2941 ggttcggcga tgcggacaaa atccacatcg acggctggca gattgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacgaaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt cgccaatgtc ggcggttatc gcgcgggtca gcttgccgag gcacacgctt
     3121 tcgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag cagcataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 gacataccta caaactcgaa atcaccgttt cagacggcat tatcaaaggc ggcgcgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacagccg cgaatgccaa atcgccgcgc
     3601 ttttggaggg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacggc cgtctgaaaa acgcggggct gaacgtgtgc cgcgtgaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt gatttatcag gaatatttaa aaattgcagc aattccaact ctctggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccttc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact cctcttcatc ctcctagtcc tcttcgtcgc cgtcgaacat
     4081 ttctacatcg cctggcttga aatgacacag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaag caagtgcaga ccttgttcag taatcaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc gccggacaac
     4261 gccgtttacg gcgcgacgac tctgtttctc ggtttcgtat tgattgccgc cgcgtggggc
     4321 gcgttttcgt ccggcaacaa aggcatactc gtcaaacaag gactgcccgc gatgctggcg
     4381 gcggcagcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccgcaatacc
     4441 gtatcgtcga aattttcgag agcctgcaag gcgaaggctg gaacacgggc atgcccgccg
     4501 ttttcgtccg cttgggcaaa tgcaatctgg cgtgcggctg gtgtgatacc gattatttga
     4561 cattcggtat gatgggcttg tccgatatct taggccgtct gaaaacctac gccgcccgca
     4621 acatcatcat caccggcggc gagccgacca tacagccgca tctcgatatg ctgctggaca
     4681 cgctcaaggc ggaaggctat ttcctctgtc tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctacgtcgcc accagcccca aagcctgcta cgccgccaaa tatgaaaata
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct
     4861 gcgaaaacat ggaacgcaaa atccgcgcac atcattacta cctttcgccc tgtgagcaag
     4921 acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattttaaac agtcgccccg
     4981 acgcatccgt gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt
     5041 ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac atacggggaa
     5101 agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg acggagcctt
     5161 actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga tggtcttttg
     5221 aaccttgccg atactctgtg ccagtgcgcg caaatggcag ggttagggaa aacgaaatgc
     5281 cgtctgaaac agcattctgt ttcagacggc atttttctgt tgccgccaaa aggaaaaacc
     5341 gcctcggcaa tggatgccga ggcggtttga atatggtcgg aatgagagga ttcgaacctc
     5401 cgcccccttc gtcccgaacg aagtgcgcta ccgggctgcg ctacattccg aattaagtaa
     5461 ggcgtgatta tagcgcaaaa agtgcggcgt gcctataccg ttttgccttt ttgccgcgtg
     5521 tcgggcggat ttaaaacgtt gtgtttgaat acagtgttga taatcatcat tatctttaag
     5581 taattcaata agataacttt ctacctgacc gaaaaaatca ttgcctttcc ctgacaaacg
     5641 gttgatgaaa tcggcagatt gttgaaacgc agccggttta aaaggcttcg ccgactttca
     5701 cgccgcccgc cgtgtcctgc ggcgaggcaa ggccggcaac aaaggcttgc gccgcttgga
     5761 aatccgccgt ctgcatcacg gcttgcgcgg cggcactgcc gagcgtgttg gccatatatt
     5821 gccaacgttg cgccaaagtg ggattgtcag gaatgcggaa atcttcgcgc agttcatcca
     5881 caaggtcggg acggttgcag acgaggacga tgtcgcaacc tgcctcaaag gaaatgcggg
     5941 cgcgttcttt gatgccgcct gccccgcacg cgccctccat agtcaaatcg tccgagaaaa
     6001 tcacgccttt gaacccgatg tcgcggcgca aaatttgttt gagccagatt tcggaaaacc
     6061 ctgcgggctt tgtgtccact tgtggataaa cgacgtgggc gggcataacc gccgccatac
     6121 cttcgcggct cataatgcgg aagggggcga ggtcggcggt ttcgagttcg gacaggctgc
     6181 gccagtcttc cggcaagacc agatggctgt ctccttcgac aaatccgtgt ccgggaaaat
     6241 gtttgccgca ggatttcata ccgccttttg tcaaaccttt ttgaagggcg agggcgaggc
     6301 gggcgaccgc ttcgggattg cggtggaaac tgcggttgcc gatgacgggg cagtttcccc
     6361 agtccaaatc taagacgggc gtgaaggaca aatcgatgcc gcaggcggaa agctcggttg
     6421 ccaaaacccg gccgacttgt ccggcggcgg tttcggcggc ggacgcgccg tctttgtccc
     6481 aaatctcgcc gagcgtactc attgcgggca ggcgggtgaa gccttcgatg aaacgttgca
     6541 ccctgccgcc ttcgtgatcg acggcgataa tgagttcggg tgtgcgcagg gctttgattt
     6601 cggcggtgag tgttttgagt tgttcgatgt tttggaagtt gcggcggaag aggatgatgc
     6661 cgcctacggc gggatcgagc aggcgttgct tttcctcttc ggtcaggcgg aaggcggcaa
     6721 tgtctgccat gacggggccg cgcggaatat gggggacggt cattgcggtt tgctccaaaa
     6781 agcttcagac ggcatatgcc gtctgaacag ggaaaggggt caggcgttgg cgcgtttttt
     6841 atctttcaac agaaaaatca gcaccgccaa tacaatgcct gtcgtgccaa agcccaacag
     6901 cgcggatttt gtcagaccca atgcgaggta gcccgatgcg gcggcggcgg caacggttaa
     6961 ggcgtaaggc agttgcgagg taacgtggtc gatgtggttg cagcgcgcgc cggtggacga
     7021 caggatggtc gtgtcggaaa tgggcgagca gtggtcgccg cataccgccc ccgccattac
     7081 tgcggacata cacgggataa tcagcgcggg ttcgactttg accgccatgg cggcggcaat
     7141 cggcagcata atgccgaacg tcccccagct tgtgcctgtg gcaaacgcca tcacgctggc
     7201 gagcaggaag aggatgacgg gcaggaagcc gggatggatg ttgcccgcaa ccagtgtgga
     7261 gaggtaatcg ccggtgtgca tttcgccgac aaccgtactg atgagccaag cgaggattaa
     7321 aatggcgatt gcgccgaaca tagatttcgc accctgccaa acggctttgg gatagtcggc
     7381 ggttttaatc gtgccgagcg tgcagagaac gacggcaagg acgccgcaag tgccgccgaa
     7441 taccagcgaa gtgtttacgt ccgtgttttc aaatgccccc aaaatgctga aggtttcgct
     7501 tgcctgcgcg ccggtgtaga tcatggcgga aaccgttgag gcgattaagg ccaaaacggg
     7561 aataatcagt gcgtaaacac gacctttggt agcgtctgaa acggcagttt catcgtgggc
     7621 ttcgttcaac gcggcttgtt cgaaacgtgc catcgagccg atgtcgaagg aaaaccatgc
     7681 gacgacgaac accataatca gggcaaacag tgcgtaatag ttcatcaggc tcatggcgac
     7741 aaacgtcccc atcggcgtgt attcggtgat tttgtaggta acgagcagtc cggcaagcgt
     7801 ggcgataatc gacgcgcccc agcttgaaac gggcatcagc acgcacatag gagcggcagt
     7861 ggagtcgagg atgtaggcga gtttggtgcg ggaaacttta aacttgtcgg taacggggcg
     7921 ggcaatcgca ccgacggcga gactgtggaa atagtcgtcg ataaaggtta cgaacacgag
     7981 gcaggcggtc agcattttcg cgccgcgccg gtttttaatg tgccgttttg cccagtcggc
     8041 aaacgcctga ttgctgccgg agtaggtcag cagggaagta aaaataccca aaagtatcag
     8101 gaaaaccaag atttttggtt tgcccagcga ccaatcgccg tctgaccaag ccaagccgac
     8161 gaccatgtct ttcaggtgtg tcagaccgtc gacggggttg ccgccgacca aaaaggcaac
     8221 gccgaccaga ataccgatgc ctaaagacag cagtacgcgg cgggtaatga cggcaagtgc
     8281 cagtgccaaa aagggtggca caaccgagaa aaatgaatgt gaatagtcga tcagctgcat
     8341 ggttatgggg gtgttaagcg tccggatggg agcgtatctg tccgcctccg gtttgggttt
     8401 tgttggcaaa atgggcggaa atattttttg tcgtaaaaaa tatttgttta aaatcaacca
     8461 actgattttt gtaaaatgcc cgttaatcgg tattgacggg cattttatca tttaaaaaat
     8521 attttggtta aattatgtgt gttattgcag gtttaatgcg ataaacagcg tgttgccacg
     8581 gcgcatgatc agcaggggga cgtttttgcc tgccttgtcc atagctttgc ggaaaccggc
     8641 ttcgtcattg acggggactt gcccgacggc aagaatttcg tcgccgcgcc tcaagcctgc
     8701 gcgttctgcc gcgtcggaaa cccgtacgac gacgaggtgt ccgccgctgc tgtcggtatg
     8761 tgtctgaagg gtaatgcctg cggattcgac cgagaacgta ccggattgct gttcggtgta
     8821 gggggcttca tctgttttgg atgatgcgcc gatatgctcg gcggcgttgc ccagcttgac
     8881 tttgattgtg atttcttcgc ctttgcgcca tacgccgagg ctgacttctt ttcccggcgt
     8941 aatggcgccg accataacgg gaaggtcgcc ggaagaacgt atttctccgc cgtcgaggct
     9001 gaggacgatg tcgcccgcct gcaggccggc acgttctgcg gggctgccgg gcaggatttt
     9061 ggcaatcagt gcgccgccgg ctttgtccaa accgaacgat tgtgccaaac cgtaggatac
     9121 ttcttgaata atcacgccca gttgtccgcg ttggactttg ccggtgtttt tcagctgttc
     9181 ggcgacattc atggcaacgt caatcgggat ggcgaaggaa atgcccatga atccgccgct
     9241 gcggctgtat atttgcgagt tgatgccgac gacctgtcct tttaagttga acagcgggcc
     9301 gccggagttg cccggattga tggcaacgtc ggtttggatg aagggtgtgt agctttcgtt
     9361 gggcaggctt ctgcctttgg cggacacgat gccggcggtc acgctgttgt cgaagccgaa
     9421 gggcgcgccg atggcggcga cccattcgcc cggtttcaaa tctttgggat tgccgatttt
     9481 gacgacgggc agctcttccg ttgcgtcgat tttcagaagg gcgacatcgg attggacatc
     9541 cgaaccgatg agtttggcgg tatattcgcg cttgtcgttg agcaggactt tgatactgcc
     9601 catgccggta acgacgtggg tattggtcag gatgtagccg tctttgctga tgatgaagcc
     9661 cgaaccgaag ttcaatccgc cgtcatctgc ttcttcttgg gggatttcgg gcatattcgg
     9721 gacgaggcgt ttgaaaaatt cgtagaacgg gtcgttgtcg gcaatcgggt cggaatcgtt
     9781 ttcggcattg ccgctgccgt tttgggtgcg cggggcgggg gctgcctgaa tattgacgac
     9841 tgccggacct tcactttgaa ccagttgggc aaagtcgggc agcagcatac tgacgctgcc
     9901 gtcgtctttg gtgtgttcga tgcgttctac gaaggatgct tcttttttgt ccgcaccgaa
     9961 aaagctgcct gccttgtcgc agcctgccag cgaggcggca cacagtgctg ccaaagcgag
    10021 gtattggtat tttttgaaca cgttttgtcc tttgtcggat gccggtaccg gctttaatgc
    10081 cgtctgaagc gcattttgtc ggcttcagac ggcataggtt gaaattctac aacgtccgtc
    10141 cgaattttca agcgtttcat tttgaagggc ggcggcggtc aggctttggc gggatattcg
    10201 cacaaatcgt tgatgatgca ggtttggcat tgcggtttga gtgccttgca ggtgtagcgt
    10261 ccgtgcaaaa tcagccagtg gtgcgcgtcc atcagaaatt ctttaggaat gaagcgcatc
    10321 agtttgtctt cgacttcgcg cacatctttc ccgggggcga ttttggttcg gttggatacg
    10381 cggaaaatat gcgtatcgac cgccatgacg ggatggccga acgccgtgtt caatacgacg
    10441 tttgccgttt tgcgccccac acccggcaat gattccaaag cctcgcggtc ttccggcact
    10501 tcgccgttgt atttttccag caggatgcgg caggtttgca taatgtgttt ggatttggtt
    10561 ttatacagcc cgatggtttt cgtgtattcc atcacgccgt ccaaacccaa atccagcatc
    10621 gcctgcggcg tatcggcaac gggaaacagc ttcgccgtcg ccttgtttac gccgacatcg
    10681 gtcgcctgcg ctgaaagcag aacggcaatt aaaagctcga aaggggagtt gaaattcagc
    10741 tcggtggtcg gatgggggtt ggcggcgcgg aagcgttcga agatttcttg gcggatgtgt
    10801 ctgttcattt ttttatacgg tgggtttgtg tgttcggcat tataacgtat ggttcaggcg
    10861 gcgtaatatt gcattcccca cagaatgaag gcgtaacgcg ccgttttgcc gataaccagc
    10921 atcagcccgc ttgtccacgg attcaaccgc agccagccgg cggcaagcgg cagtgcgtcg
    10981 ccgacgacgg gcagccaggt aaacgcaagc agccaaatac cgaaacgccg catcagattc
    11041 agtgtttttt cagacggcat ttttcgggag ggcagcaaac gccccatcca ataggaaacc
    11101 atactgccca atccgttggc aaggccggcg cacagcaacg cgccgtatgc gtgttcggga
    11161 aagcggtgga cgaacagggc aaaggcggct tcggatgtgc cgggcaggag ggtggcggaa
    11221 gtgaatgcgg aaaaggcgag ggcggcgtag gtgtaggagg gtatcattgc aaacagtctc
    11281 aaacaggtaa caatcggcga cggattgtac
//
LOCUS       DAWISC010000050        11303 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWISC010000050 DAWISC010000000
VERSION     DAWISC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.050"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(80..1126)
                     /gene="recA"
                     /locus_tag="WIW87_000951"
     CDS             complement(80..1126)
                     /gene="recA"
                     /locus_tag="WIW87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ5174130.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1380..2144
                     /locus_tag="WIW87_000952"
     CDS             1380..2144
                     /locus_tag="WIW87_000952"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ5174131.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2164..4179
                     /gene="rep"
                     /locus_tag="WIW87_000953"
     CDS             2164..4179
                     /gene="rep"
                     /locus_tag="WIW87_000953"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ5174132.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYQLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQTFMDMFVSYLAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WIW87_000954"
     CDS             complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WIW87_000954"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ5174133.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGDVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVSPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(5449..8538)
                     /locus_tag="WIW87_000955"
                     /pseudo
     CDS             complement(5449..8538)
                     /locus_tag="WIW87_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8812..9591
                     /locus_tag="WIW87_000956"
     CDS             8812..9591
                     /locus_tag="WIW87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ5174134.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(9802..11214)
                     /locus_tag="WIW87_000957"
     CDS             complement(9802..11214)
                     /locus_tag="WIW87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ5174135.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEISALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT         2756 a         3170 c         2733 g         2644 t
ORIGIN      
        1 aactcaaatc aagccgttcg gaggcagctc aaaaaacggt acttcgcagc agaagtaccg
       61 tttatcggga tttcaggttt tattcttcgg ggcgttcgcc gtcggtttcg tcctgcgtcc
      121 cttcggtgat gtgcatttct atgccgttga gggcgcggat ttttgcgtcg atttcgtcgg
      181 caatttcggg attttccttc agccagacgc ggacgttgtc tttgccctga ccgattttcg
      241 cgccgttgta gctgtaccac gcgccggatt tgttgatgat gtcgtttttc acgccgatgt
      301 cgatcaattc gccttcccaa ctgatgcctt ctccgtagag gatgtcaaac tctgcctgac
      361 ggaacggcgg cgcgactttg tttttgatga ctttgacgcg ggtttcgttg cccaatactt
      421 cttcaccttt tttgatggat ccggtgcggc ggatgtcgag gcggacggaa gaatagaatt
      481 tcagcgcgtt gccgccggtc gtggtctcgg ggctgccgaa cattacgccg atcttcatcc
      541 ggatttggtt gatgaacaca accagcgtgt tggttttttt gatgtgtccg gtcagtttgc
      601 gcaaagcctg gctcatcagg cgcgcctgca gtccgacatg gctgtccccc atatcgcctt
      661 cgatttcggc tttggggacg agtgcggcta cggaatcgac gactaccata tctatgccgc
      721 ccgaacggac gagtgtgtcg cagatttcca aagcctgttc gccggtatcg ggctgggaca
      781 ggtaaagctc ttcgactttt acgccgagtt tgcgggcgta aacgggatcg aaggcgtgtt
      841 cggcatcgac aaaggcgcac acgccgccgt ttttctggca ttgggcgacg gcttcgaggc
      901 agagggtggt tttgccggag gattctgggc cgaagatttc gacgatgcgc ccgcgcggca
      961 gaccgccgac tccgagggcg aggtctaatc cgagcgatcc ggtggaaatg acttcgaggt
     1021 tttcttcctg ctggctgccg tccattttca tgatggcgcc tttgccgaaa cttttttcga
     1081 tttgcgccag tgcggcggca agtgctttgc ttttgtcgtc tgacattggg gttactccgg
     1141 aacaaatgcg gtatgtggga tgcggcgcaa cacgggctgc ggcgcgggat gtgtatcgtt
     1201 ttcccgatgt gcgggctatc ggtaatgctg cttcacgagg ttgccattat cgcatatttc
     1261 cttgcttgcc gatatgcgcc aggacgcggc ggcttgtgcc ggaatggaat ctggatgccg
     1321 tctaaaaggc ggccggcttt gttataatgg cggctgtttt ttctgtgtgt gcctgtttta
     1381 tgtgttcctg ccttgttgtc aaaaataccg ttatcggaag cggacgcacc aaaatcgccg
     1441 tgccgcttgt cgcccgcgat gccgccgaac tttccgccgt acttgagcaa atcaaaaata
     1501 tgcccttcga tattgcggag ttccgcgccg actttttgga atgcgcgggc agtatcggcg
     1561 aaatattgca ccacacgcag accgtccgcg acgcgctgcc cgacaagccg ctgctgttta
     1621 cgttcagacg gcatggcgaa ggcggctcgt tcccgtgttc ggacgattat tattttgaac
     1681 tgctcgacgc gctgatcgaa agccgcctgc ccgacatcat cgacatcgag ctgttttccg
     1741 gcgaaaccgc cgtccggtgc gccgtggcaa atgctcaaaa aaacggcatc gccgccctgc
     1801 tctgcaatca tgagtttcac cgcacgccgc cgcaagaaga aatcgtatgc cgtctgaaac
     1861 agatggagga ctgcggcgcg gacatctgca aaattgcggt gatgccgcaa agcgcggaag
     1921 atgtgctgac tttgctttcc gccacgctca aagcgaaaga gcttgccgcc aaaccgattg
     1981 ttacgatgtc gatggggcag acgggggcgg tcagccggct tgccggacag gtgttcggct
     2041 caagcatcac gttcggttcg ggaacgcaaa actccgcgcc ggggcaaatc ggcgtatccg
     2101 ccctccgtgc gacactcgac tgcctcgaaa acggcgcaga ctgatttcag acagcatcaa
     2161 aacatgatga aactcaatcc ccaacagctc gaagccgtcc gctacctcgg cggcccactg
     2221 ctcgtccttg ccggtgcagg cagcggcaaa accggcgtga ttactcaaaa aattaagcat
     2281 ttgattgtca atgtcggcta cctgccgcat accgttgccg caattacctt taccaacaaa
     2341 gccgctgcgg aaatgcagga gcgcgttgcc aaaatgctgc ccaaaccgca aacgcgcggg
     2401 ctgacgattt gcacgttcca ctctttgggc atgaagattc tgcgcgaaga ggcgaaccat
     2461 attggttaca aaaaaaactt ctccattctc gattctaccg acagcgcgaa aatcatcggc
     2521 gaactcttag gcggtacggg caaagaagcc gtattcaagg cgcagcacca gatttccttg
     2581 tggaaaaacg atttaaaaac gcctgaagat gtcgttcaga cggcatcgaa catttgggaa
     2641 caacaaaccg cacgcgtgta tgcgagctat caggaaacct tacaaagcta tcaggcagtg
     2701 gacttcgacg acttaatccg cctgcctgcc gtgctgttgc agcaaaacag cgaagtgcgc
     2761 aacaaatggc agcggcggct gcgttatctg ttggttgacg aatgccaaga tacgaatacc
     2821 tgccaattta cgttgatgaa gctgctgacc ggcgcggaag gtatgtttac cgccgtcggc
     2881 gacgacgacc agtccatcta cgcatggcgc ggtgcgaaca tggaaaacct gcgtaaaatg
     2941 caggaaaact atccgcagat gaaggtcatc aaactggagc aaaactaccg ctccaccgcg
     3001 cggattctca aaatcgccaa caaagtcatc gaaaacaacc ccaagctgtt taccaaaaaa
     3061 ctttggtcgc aattgggcga aggcgagccg gtcaaagtcg ttgcctgcca aaacgagcaa
     3121 cacgaagccg actgggtcgt cagccaaatc gtcaaacaaa aactcatcgg cggcgacaaa
     3181 acccaatatg ccgatttcgc cgtgttatac cggggaaagc atcaggcgag gattttcgag
     3241 gaagcattgc gcggcgcgcg catcccctac cagctctccg gcggacaaag ctttttcgac
     3301 aaagccgaaa tcaaagacgt gttgtcttat gtgcggctgc ttgccaaccc caacgacgat
     3361 cccgcctttc tgcgtgccgt taccacgccc aaacgcggca tcggcgatgt cacgctgggc
     3421 aagctcaaca cttacgcgca cgaacacgaa tgcagcctgt atgaagccgc gcaaaacgaa
     3481 gaagcccttg ccacgctgaa caataccaac cgccaacacc tgcaaacctt tatggatatg
     3541 ttcgtcagct acctcgccaa agccgaaacc agcgaagcgg gcgagttcat caacagcctg
     3601 ctcgaagaaa tcgactatga aaaccatttg atgcaaaacg aagaaggcaa agccggcgaa
     3661 atcaaatggc gcaacgtcgg cgatttggta tcatggtttg cgcgaaaagg cggggaagac
     3721 ggcaaaaaca tcatcgaact cgcccaaacc gtcgccttga tgacgctttt ggaaggaaaa
     3781 gacgaagaag aaaccgatgc cgtctcgcta tccacgctac acgccgccaa aggtttggag
     3841 tatccgtatg ttttccttgt cggttgcgaa gaaggcgttt tgccgcacaa cgacagtatc
     3901 gaagagggca acgtcgaaga agaacgccgc ctgatgtacg tcggcatcac ccgcgccaaa
     3961 cgccaactca cactgaccca ctgcgtcaaa cgcaaaaaac aaggcacatg gcagttcccc
     4021 gaacccagcc gattcataga cgaaatgccg caggaagatt tgaaaatcct ggggcgcaaa
     4081 ggcggcgaac cgattgtcag caaagaagaa ggcagacgca accttgccga tataatcgga
     4141 aggctcgaca acctaaaaaa aagcggcgcg gcggattaaa ccggagccgc aatgccgtct
     4201 gaaggcttca gacggcatat tttttggacg gcgcgcgtaa agcggtttac gcccacaaat
     4261 cctgctgctg gtttttcggc acaagatgcc ccacgccgat accgataagg cggaacgcgt
     4321 cttccgtctg cggcgagacg cgcgccatca acatttgcgc agcctgcagc agagctgcgc
     4381 agtcgggcaa tacggaggaa taagtcagtg tgcgcgtgat gatgcggaaa tcgtaggtct
     4441 tcagcttgag cgttacgctt tgggcttcga cgtttttgcg cgtgatttgc cgccacaagt
     4501 cttcggcaag atgggggagg tgtccggcag cctgctcgag cggcaggtct tcgggcaggg
     4561 taatttctgt ggagatttgg aggcgttcgc gttcggcttt gacggggcgt tcgtccgtac
     4621 cgcgcaccaa atcatagagg cggtatccgt agcgtccgaa atggtttaag agttcgccgc
     4681 gctcgaaacg gcgcaagtcg cccgccgtcc gcatacccag cgactgcatt tttttcagcg
     4741 ttaccttgcc cacgccgggg attttgccca aaggcagggt ttccaaaaat gccatgactt
     4801 tgtgcggcgg caacacaaac tgcccgttcg gcttgcgcca gtccgacgcg attttcgcca
     4861 gaaatttgtt cggcgcgatg cctgcggatg cagtcaaacc tgtttccgca aaaatggcgg
     4921 cacggatttc tttggcaacg tcgccggcgt aagggatgtt tttgaaatta cgggtaacgt
     4981 caagataggc ttcgtccagc gacaagggtt cgattaaatc ggtataacgc ctgaatacgg
     5041 cgtgaatctg cgcggaaacc tgacggtaca aatcgaaatg cggcggcaca tacaccgctt
     5101 gcggacacag ccttttcgcc gttgccaccg acatcgcgga atgcagcccg aactgccgtg
     5161 cctcatacga tgcggcgcaa atcaccgaac gcgcgccctc ccacgcgacg accaccggcc
     5221 gccctttcaa atgcggctgt tcgcgcagct ctaccgatgc gtagaatgcg tccatgtcga
     5281 tgtggataat tttgcgtgaa gacatcggct cttctgagga taaaagggat attctactgc
     5341 cggcatcggg caaattccaa atatacgccc cgatagacct gcctccataa aaatgccgtc
     5401 tgaaacatac cctgtttcag acggcatccg caaaactacg gttttcaatt aaaactgcca
     5461 atccagtttc atgctgacag tgcgcggctc tccgtagaag ttgtttgcgc cgcgcgtacg
     5521 gttgtagttg ttctcaaaat aagtgcgtcc gtttaagttc gtaccgatga ggctcaattt
     5581 ggcgtgtttg cccaattcgt aacggacgaa accgtctatc agcccgtagc cgccctgcct
     5641 gatgttatac agactgcttg tgccgctttg tgcggacacg ccgccgccga cggtcagccc
     5701 cgtattcggt atatggaagc tcgttccgaa acggaatatg tgcacgggtg tgaaattgct
     5761 gaagttgtac gggtctgcac tggaattttt ggcaaggcgt tcggcgttga cttcggcggc
     5821 gtttttgtag cggctcttgt tgtaggtgta acccgcaaag actttccaat cttcgttcaa
     5881 ctcacccgac aactcgaatt ccgcacccct gctgaccact ttgcctatcg gtttggcaac
     5941 ggtttggaac gacccctgct tgccgcctgc tccgggaaca tagccgaaat cgacgaccgt
     6001 gcggtttttc tgttcgaggt aaaacaatgc gaacgaagca ttcagccgtc cttgcaagaa
     6061 cgcgcctttc cagcctacct catagtttgt gccgaccaaa ggcggtaaaa cggttttggc
     6121 actgacatcg acattatcct gctgtttgaa gattttggta taacttccgt aaatactctg
     6181 ttgcggtgtc aagtcatagg taatgcctgc atagggcgtc aatttatgac cttgcatctt
     6241 ggccgtgtaa tggtcctgat ccgccctaat gctcgatgcc gtctgaaaat cgcttgccgg
     6301 ctgcccatag cggacaggca tatctttggt ttgcgaagtc tcatagcgcg tgtagtgcag
     6361 cccgcccaaa aggtgcagtc ggccggttac gttgaaacgc gtgctggcag tcagcgaatg
     6421 ggttttgttg gtgttgaggt atttggcgta gttatacagc gcaggaacat ggtcgtctgc
     6481 cactttgacg gttttccaaa ccggcaccgt accggaaaaa ccggtaaagg caggcgtgcc
     6541 gtcgggattg gtctcctgaa tcttgttgcc tttttcgtcc agctcatata catcgacata
     6601 taccggtgtc cggctgccgc tgtattcgtc atagtaatac acctgcttgc cttcggcatc
     6661 gagcttgggc tcggttttta ttttcttggc gttcctgcat tcttcggcat aaacggtacg
     6721 gttgcctttt tcatcgtacg cctgccaatc gggttcttta tgccccctga ccaaaggaga
     6781 cgacaaatcg ccgtccggct cctcctgaca acttcccgca tacacgccgt gcgttgcccc
     6841 cgtattcgga cgtactctgt agcggcgttc gtagatttct agatattccg aacgtatctt
     6901 ttcatcaccg taggcatagc cgacaaagaa atcatgctcc cgcccgaaca gcccatatgt
     6961 gccggtcagg tcaagtttaa ttccccattg gcggtcgtct ttggtatgcc gcaacggcat
     7021 ataactgtat cgtcggttgg cggtagcctt ccgattaaag gaagagtcat acaggctgtt
     7081 tggaaaacgt tgtgccgcat tattaaagat gccctccttc gcaagggctt tatcgacaaa
     7141 ttccgccttg tcggcatcaa cgcccggatc cccccaagaa ccttgacaga taaagtccag
     7201 cgcgaaaggg tcactcatac acttgtcaaa accggctttg cgttctgcgg cacggcggct
     7261 gcgatactgt tcgaaagcag tattatcgaa acggttttta acaaaatcgt ctttgcgctc
     7321 ccggtattcc ttggcggttt catcacgata tgctttcagt ttctccaatg ccttatcttt
     7381 cggctcgaac gggatgactt cgtttttttc agtcaaaaag cctaccgcat cctcacccga
     7441 caaacccgcc gcatattcgt ttttcagaaa aaactgcccc accttcgcat cggattcatt
     7501 cttggtataa gacacttcgg cattgagctg ccaaccgttg tcaaacacat gtttgaatcc
     7561 tgagaaaagg ttgtatttgt cggcacttaa ccgcgaccaa tcctccccca aataagtgtt
     7621 gcgcggcagt tgcaaaggcc ggttgcaggc aggcgttgaa ctgaacgggg cagttttctg
     7681 attttcacag ggcaaaataa tgcccgaaaa atcaggaacc tccctactct tctgatacat
     7741 gccgcccaaa gtaagcacac tgctgtcgcc cgcatcggct tcggcaatgc cgtaaaccat
     7801 atgtttcctg ccccaaactc ggtctttaaa cgatttttta tactcttccg cacccaccaa
     7861 ccttccgcgt aaggtattcg ccttattcag gctgcctgaa acatccaaca ctgcacgccg
     7921 gctgccgcga tggtcggcgg tcagctctcc ggtatgtttg aaagaagcgg taggtcactt
     7981 acggatcaaa ttgacggttc ctcccggctc tgaattggat tgggtcaacc ccgttgcacc
     8041 ccgtacaact tcaatatggt cataaaccgc caaatcggta ctcggagaca cgtcgatttt
     8101 cgccgtatat cccgaacggc ctgcaacatt gacggtcata ccgtcttcac caatctgatc
     8161 aatatagaaa ccgcgtgaca aaaaccgcgt ctgcaagcct gaatcgcgca caacgttgac
     8221 acccgtcgtg tttttcattg cctcttcaag cgtatgcacc gccttatcgt caaggcggct
     8281 gcgcgtgatg acgctgaccg actgcggcgt atccttgccc gcaatcctca tacctgtggc
     8341 ggtggacatc cgatctatcg tataagaacg ggtcttttcg gtcttgccca acaaagcatg
     8401 agagccgcgt acattgaccg tatccagact gacggtattg ccgtctgaaa caggcacaac
     8461 accgtctgca aaagaaccac cgtaagccga taacagcata acggtcagaa ttttaagtga
     8521 aaaatgattt tgattcatag agacctctgt aatatgcaag tgtgcaaatc gtccaaaggc
     8581 tctcacaact gttttgattt tttatattaa ttgaaaaaaa gtaattctca attaaattta
     8641 tagataggat tgtattccca ttttgacaaa aaacaaacta ctccttaccg tttatttcaa
     8701 aaaacgataa cattgtattg aaaaatatcc gaatttaaat acagaccgcc aatgcagaaa
     8761 aaaacaccca aattggctat aatcccgaca aacacactca aggacaacaa catggcagcc
     8821 tcgcccgaag caaaattcac cgaagaaaag attttgtggg tcaaacacca cacgccgaaa
     8881 ctcatcactt tcgccatcag ccgtcccgaa tcctaccgct ttaaagccgg acagttctcc
     8941 cgactcggtt tctacgaagg ggaaggtttc atttggcgtg cctattccat tgtttccgca
     9001 gaatatgccg acacgctcga atattttgcc gtactcatcc aagacggccc catgtcggcc
     9061 cgtttcgcca aaatgcaaca gggcaacacc atcctgctcg ataaaaatgc caccggcttc
     9121 ctcctgcccg aacgcttccc cgacggcaag gatttggtga tgctctgcac cggctcgggc
     9181 atcgccccct tcctttccat tctcgaacaa cccgaaatcc gtcaacgttt cgataccgtc
     9241 aacctgatac attccgtatc ttttcccgaa gaattgattt tcaacgaccg actcgccgca
     9301 ttgactgaac atcccctggt aggcgaatac ggacactctt tccgtttcgt ccctgttacc
     9361 acccgtgccg ccaacccctc gggcttaagc ggaaaacgca ttccggaact cttaaaaaac
     9421 aacagcatcg aacaggcgct gcataccaag ttcaccccgg aatccacacg gtttatgatt
     9481 tgcggcaacc cggaaatggt caaagacact ttccaaacgc tgctcgacat gggttacgcc
     9541 atgcaccgca accgcattcc cggtcaaatc atgatggaaa acggcttcta aaaaccaccc
     9601 tgcttgtccg atgccttcgg atggacgggc aaaccgacac ggcacgaaaa ccgcgtcggc
     9661 aaaaatgccg tctgaaaaaa ttcagacggc atcttcggat acattacctg caaacggcaa
     9721 cacaccggca caaaccgatt aggcaatcaa cacggtgacg gctgtttaca tacttgccgg
     9781 ctttcaccaa ccgatatcga tttaaccgat ttccttaata tttttcctgt ccgttttaaa
     9841 cttcgcctta aacgcatccg gtaaatcttt atcgaaatac caaagcccgt catccatttc
     9901 caatgcgccg cccattccgt gcagaacgac tttttccccc gtcaaaggat cggtttcggt
     9961 aatttccacc tcatgcattt ttccccaatc caaaacaggc aacttgtgtg caaacgccaa
    10021 aacctgttcg tcagaacggc cgcacgcctt atcgcatcgg cctgaaacat atacgggaca
    10081 aggctcatcc tctgcataac cgtccggaag gataccggct gcggcaggac acaatccgta
    10141 tgtttccgcc cacgacgaca aagcccgtct cgctgccttt ttattggcaa ataatccggt
    10201 aggcggatta tccgtcacac cgtttttcaa agccgctgtt ttcgcattca acatgccgtc
    10261 tgaacctttt tcaaacctga cggtcgtaaa tgttttaagc agatttttgg cagacacata
    10321 acaatccgaa tgataacgcc cgaccaattc cgctttaatc ttatatgcat gcaggctgcc
    10381 caatgcggga aaaaaacgga cttcctccgt attgcaccaa tcaaacgggg cttttccgga
    10441 gtccaataaa gccgaaatct cgctatatac ccgttcaaac gtaccgatat aatttacttt
    10501 ccctccgccg tcgaaacaag ccagcacccc cataccgtca ggcaaaccgt acaactgttc
    10561 cctcaaccgt tcgggcagcg cggcaggcag cggtttcgga ttcatcaaac ggaaacactg
    10621 cctgatccat gcctcaaccc cgtgttccga cagactgtat tccaaataat cacacaatgc
    10681 cgatacatcc gccatcgcac gatgcctgtc ttccacaaca atccccaacc tttcgatgat
    10741 actgtccagg ctgtgcttgt aaaattgcgg atacagacac cgggacagct gcacactgca
    10801 caaagcaggc gatgaaaatc cgatacccgc acgatgaaac tcatgcttta aaaacgtata
    10861 gtcgaaacgg ctgttatgtg caaccagcac acaacccttc aataccgaaa acaactcgcc
    10921 ggcaatctct gcaaaaacag gcgcatcggc aaccatgccg tctgaaatcc ccgtcagccc
    10981 cgccacaaac tgcggaatcg gtttttgagg attaaccaac cactcatgcc tcaccaccct
    11041 tccctgctca aacttgacca aagccacttc ggttaccctg tcttcataca gattgccgcc
    11101 cgtcgattcc aaatcaacca cggcaacagg cattccaaac cgtaaaaata ccttttccag
    11161 caagggccag cgagaagcaa caatcatttt attctcttta aattcaaaca acaaaccaat
    11221 attttacact tttaaggcat ttcatccaac aaaacaattg acagaatccg atgattaccc
    11281 taaaattcga atctttcttg cag
//
LOCUS       DAWISC010000051        11221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWISC010000051 DAWISC010000000
VERSION     DAWISC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.051"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WIW87_000958"
     CDS             complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WIW87_000958"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ5174136.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTASKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1204..2073
                     /gene="tehB"
                     /locus_tag="WIW87_000959"
     CDS             1204..2073
                     /gene="tehB"
                     /locus_tag="WIW87_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ5174137.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WIW87_000960"
     CDS             complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WIW87_000960"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ5174138.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLEFDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2990..3187
                     /locus_tag="WIW87_000961"
     CDS             2990..3187
                     /locus_tag="WIW87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174139.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(3407..3482)
                     /locus_tag="WIW87_000962"
     tRNA            complement(3407..3482)
                     /locus_tag="WIW87_000962"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3446..3448),aa:Glu,seq:ttc)
     gene            complement(4202..5614)
                     /gene="trkA"
                     /locus_tag="WIW87_000963"
     CDS             complement(4202..5614)
                     /gene="trkA"
                     /locus_tag="WIW87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ5174140.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(5712..7235)
                     /locus_tag="WIW87_000964"
     CDS             complement(5712..7235)
                     /locus_tag="WIW87_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ5174141.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(7542..8348)
                     /locus_tag="WIW87_000965"
     CDS             complement(7542..8348)
                     /locus_tag="WIW87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ5174142.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(8400..8651)
                     /locus_tag="WIW87_000966"
     CDS             complement(8400..8651)
                     /locus_tag="WIW87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174143.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            8751..9512
                     /locus_tag="WIW87_000967"
     CDS             8751..9512
                     /locus_tag="WIW87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174144.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            9657..10826
                     /gene="metZ"
                     /locus_tag="WIW87_000968"
     CDS             9657..10826
                     /gene="metZ"
                     /locus_tag="WIW87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ5174145.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSAGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVAKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELLAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLSDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            10913..11119
                     /locus_tag="WIW87_000969"
     CDS             10913..11119
                     /locus_tag="WIW87_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ5174146.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2415 a         2742 c         3266 g         2798 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccctttaggg cggcaggatc agactttatt tgggaggggg tgtaacccct
      121 tccaaatcag gacgacacat agggaggtgc tttatgtgtc gtcctgtgtg ttgaaacata
      181 tcggacgcgt ttgaacagtt gtgcggcaaa ctgttttagc ggcaggcaag agatgccgtc
      241 tgaagcccat tgtccgtcat tcccacacag gcagttgcta cggttgctga agccccttag
      301 ccgtcgcgca ggcggttgct acggttacta aaatcccgtc attcccgcgc aggcgggaat
      361 ccagtccgtt cggtttcagt tgtttctgat aaatcttgca gcgttgggtt tttagattcc
      421 cgctttcgcg ggaatgacgg ggcggtcgga tgccgcctga aactccgtca ttcttgcaac
      481 ttttcgtcat tcccgtgaaa acgggaatct agaacttcaa actttcagat aatctttgaa
      541 tattgccgtt gcccgacggt ttggattccc gcttgcgcgg gaatgacggt ggaggacgat
      601 gccgtctgaa gcccattagc ggtttcagac ggcatttttg ccggagcggc agtcaggaaa
      661 actgcgctgc gccacggttt gccaaatcgt cggcgcgttc gttttcggcg tgtcccgcgt
      721 gtcctttcac ccaagtccaa ctgacttgat gccgtccgac tagagcgtcg agttctttcc
      781 acaagtcgtc gtttttgacg ggctgtttgg aggcggtttt ccagccgttg cgcttccaac
      841 cgtgtatcca gttttccatg ccatttttga cgtattgcga gtcggtgcag atgatgacgg
      901 tgcagcggcg tttgagcgat ttcagtcctt cgatgacggc agtcagttcc atgcggttgt
      961 tggtggtttg cgcttcgccg ccgaaaagtt ctttttcgtg gctaccgtag cgcattaaca
     1021 cgccccagcc gcccgcgccg ggattgcctt tgcacgcgcc gtcggtgtaa aggtaaacgg
     1081 tttggttcat tattgtgcct ttgtgtggtg tccggcgtga ttataagcgg tttgcgcgcc
     1141 gtgccgtctg aaagattgcg gcggattaaa ataggcatat aatgtgcaac tttatttttg
     1201 aatatgaagg agcggatagt gggtcaaagt ggcgagttgt tttgtttcgg acagatgcct
     1261 gtatggaaag tggaaaacct gccggaagtt ttgttgtcgg gctattcgtc tgaggaaggg
     1321 gagtgggtct gcctgaatgt gttgcagggc gatgtcgaag ttcgtgcacc ggacgggtcg
     1381 gcagaagttt ggtcggcaga cggcggcgat tgtgtgtttg cgccgcagca ggtgttttct
     1441 gtcaaaccga aaacggacga tgccgaaatc cgtttgtcgc tgtattgcgc ggcggcagac
     1501 tattttcaca aaaaatacgg gatgagtgcc acgcattctg cggtcgcggc ggcacgggat
     1561 accgtaccgg cgggcagggc gttggatatg ggctgcggac aggggcgcaa cgcgctgttt
     1621 ctcggtttga aggggtttga agttactgca gtcgatcaca atcccgccgc cttggcaaac
     1681 gtggcggagc tggcagaggc ggagggtttg aacgtccgca cgctggaata tgatttgaac
     1741 gccgccgcct tgcagggcga gtttgattat attgtggcaa cagtggtgct gatgttcctg
     1801 atgccgcagc gcgtacccga cgtgattgcc gatatgcagg cgcatacggc ggcgggcggg
     1861 tacaacttga ttgtatcggc aatggatacg gcggatttcc cctgtccgat gccgttccct
     1921 tttaaattta aagagggcga gctgaaggat tattatcggg attgggaact ggtcgaatat
     1981 aaagaagaat tgggttctat gcatgccaaa gacgaaaacg gcaatccgat acggtttaaa
     2041 tttgtaacga tgctggcgaa aaagcctgaa taatcctcaa aaatgccgtc tgaacatttt
     2101 gtttcagacg gcatttttac ggttagtccc gatacgccca atgattgacc gggccgtgtc
     2161 ctgcgccgat ttccaaaggg tttgagattg ccgccgtgat gtaggccttg gcagtctgta
     2221 cggcttcgca aacgtccgag ccttttgcca actcggcggt gatgcaggcg gaaaacgtgc
     2281 agcccgtgcc gtgcgtgtgg gcggtcggaa agcgcgggct gtcgaattcc agcgtttcat
     2341 tttgtgtaaa cagccaatcc gtgcagcgtc cgcttgtgct gccgttcaaa tgtccgcctt
     2401 tgataacgac atttttgaca ccgtaatcaa gcaggatttt tgccgcacgt tccgcatctt
     2461 tacggttttc aatatgcacg ccggtcagag cttccgcttc gggcaggttg ggggtcaata
     2521 catccgtatc gggaagcagc aggcgcgtca gtgccgcaac ggcggaatcc tgcaacagcg
     2581 gcgcaccgcc tttggcaatc atcacagggt cgagtacgcg cctgccaaag ctgcagtgtt
     2641 tcagcttgtc ggcaacgcat tcgatgattt ccgccgtgcc gagcataccg attttgtagg
     2701 cgcggatgtc gaagtcttcc ctgattgctt ggatttgtgc ggtgatggtt tcggtcggga
     2761 cgagatgaac cgccgacacg cccaaggtat tttgcgcggt aacggcggtg atgacgcacg
     2821 ttccgaacac gccgcgcatc tgaaatgttt tcaaatccgc ctgaatgccc gcaccgccgc
     2881 ccgaatccga accggcgatg gtaagcgttt gcacaaaaga gcttttcatt tgttcctcct
     2941 taatattgcg tccgataaaa agggctaatg cctgtaaaaa agcacggcga tgccgtctga
     3001 aaaccgcagc gcgggggttt gtggtgcatt atatgccgta ttgcaggaca aggcacaggg
     3061 cagggcggcg gggtgcatcg tagcaactgt atttttgccc gacggggtga aaaatacagt
     3121 tgctacaccc actgataatg aaaaaagaga gcaatgccgt ctgaaccgcg tgttgcggcg
     3181 agcataaacc ggcacgcttt attcgtcatt tccacaaaaa cagaaaccta aaattccgtc
     3241 attcctacga aagcgggaat ccagacctgt cggcacggaa acttatcgga taaaacggtt
     3301 tcttcaaccc tacgctctag attcccgctt ttgcgggaat gacgaaaaat tgcgggaatg
     3361 acgactcata ataaaaaacc tgcttgaaac aagcaggttt ttgaattggc acgcccacgg
     3421 ggattcgaac cccggttgcc gccgtgaaag ggcagtgtcc taggcctcta gacgatgggc
     3481 gcgtggaaac ggacattata catacttata gaaatttagc aatacgatgc cgtctaaaca
     3541 aatgtcaggg gggcgtttgt ctgatcgcat cacaatataa aaacatataa aaaacattgg
     3601 aatatcaagc aattcatttt tttgcgtggt aatgccgaca acggaaggca gctttatttt
     3661 gaggcgcaaa ggtcttcgat gcacacgatg cgctagactt ggcaggtcaa tatgggcgtg
     3721 cgcagtatgt tctgatgcgg cggattcgcc tgaaaacaag ccggaacggt cagatgcgtt
     3781 ccggattgtt tttttgtcgg gggggtgccg ctatatcaaa acaggcgggc gatgccgtct
     3841 gaaacttcgt cattcccgcg aaagcgggaa tctagaactt ttaaattttc agacggcttt
     3901 tgaatattgc cgttgcccga cgttctggat tcccgcaact tttcgtcatt cccgcgaaag
     3961 cgggaatcta gaacctctaa attttcagat aacctttgaa tattgccgct atcccaaggt
     4021 ctggattccc gcctgcgcgg gaatgacggg tcttttataa cctttgaata ttgccgctat
     4081 cccaaggtct ggatgcccgc ttacgcggga atgacgggga gggcggatgc cgtctgaagc
     4141 ccacctacca ttatttccgc ttccataccg cttggcggca atatgtccgg aatggggcgg
     4201 tttatccgaa aaagcccatt ttgacctgga tgagtttttc cagttcgttc aggatgcgcc
     4261 ggcgcgagac gaaaaagatg atgtggtcgc cgtcttggat gacggtttcg gtatggtgtc
     4321 ccataatggt ttcgccggtt ccggcgcgga cgacggcggc aatgtggcag ccttcgggcc
     4381 atttgatgcc gctgatgcgc ctgccgatga tggcggaagt ttttttgtcg ccgtgtgcga
     4441 cgacttcgat ggcttccgcc gtgccgcgcc ggatggggtg gacggcaacg atgtcgccgc
     4501 gccggatgtg ggcgagtatc gagccgatgg tgatgaggtg gggggagacg acgatgtcga
     4561 ttttgttgcc ttcgagcaaa tcgacgtagc ttgagcggtt gacgatgccg atgacgcgct
     4621 tcgcgccgag gtttttcgcc aaaagggcgg acataatgtt gctttcgtcg tcgttggtca
     4681 gggcgcagaa tacgtcgatt tcgtcgatgt attcgttgtc gagcagggtt tcgtcggttg
     4741 ccgaaccttg caggacgagg gtgttgtcga ggttttcggc tatccattcg gcacggcgcg
     4801 gccggcattc gatgattttg acgttgtatg cgtgttcgag ctgcttggcg agacggtagc
     4861 cgatgttgcc gccgccggca atcatgatgc ggcgggtgct ggtttctttg gggcgcaatt
     4921 cgggtatgac cgcgccgatg ttttcggcgg cggcggcaaa taggatttcg tcgccttcga
     4981 tgatgacggt ttgcggcgcg gggacgatga ggcggttgtt gcggtaaacg gcgcagattt
     5041 ggcagtcggc cccgtcgggc aaatcttggg cgatgtcggc aatgctgcgt ccgacaagca
     5101 gtccgccgcg ccgcgcctgt atgatgacca tccgcacgcg gtcgtctgca aaacgtaaaa
     5161 cctgcaatgc gcccgggcag tctatcaggc cggcaagctg ttcggtaacg agctgttcgg
     5221 ggctgatggt ttcggttatg ccgaatatgg aaaggctgcc gttttcgttg ttttcgagct
     5281 tggggctgag gtattcgagg tattcgctgg aacggacgcg cgcgatgcgg ccggggatgt
     5341 tgaacaggtc ggcggcaact ttgcaggcga cgatgttggt ttcgtcgctg cgggagagcg
     5401 cgagcagcaa gtcggcatct tccgcgccgg cgcgttctaa tgtgaagggg gatgcgccgt
     5461 tgccgaaaac ggtttggaca tcgaggcggc tgcctgtttc ctgcaatgct ttttcgtcga
     5521 tgtcgataac ggttacgtcg ttgttgggta tggcggcaag gttttgtgcg acggtagaac
     5581 ctacctgtcc gttgcctaaa atgaggattt tcacggtgtg ggtcgccgag tgatgaaatg
     5641 gtggattgta ccgcaaccgg gggcggaaat gccgtctgaa ggcagatttt gtgttcagac
     5701 ggcatggcgc ctcaagattc gacggggatg atgccgattt tcgcctgcca tttgcgcggg
     5761 gcggtggcgt ggatggattc gcctttgcta tctacggcga cggttacggg catgtctttg
     5821 acttcaaatt cgtaaatggc ttccatgccc aattcgggga acgccaagac tttggaagat
     5881 ttgatggctt ttgccacgag atacgccgcg ccgccgactg ccatgaggta cacggctttg
     5941 ttgtcggcga tggcttcgca ggtggccacg ccgcgctcgg atttgccgat catgcccaag
     6001 aggtcggttt gttcgagcat ttggcgggtg aatttgtcca tgcgggtggc tgtggtcgga
     6061 cctgccggac cgacgacttc atcgccgacc ggatcgacgg ggccgacgta gtaaatcagg
     6121 cggttggtga aatcgacggg caattcttcg cctttgttga gcatatcgac gaggcgtttg
     6181 tgtgcggcat cgcggccggt gaggattttg ccgttcaaca gcaatacgtc gccggttttc
     6241 cagcttgcca cttcttcttt ggtcagcttg tcgacatcga cgcgtttgcc gttgtcgggg
     6301 ctgtaagtca aatcgggcca gtcttcgacg cgcggcggcg tgagttcgac agggcctgag
     6361 ccgtccaatt caaattcgac gtggcgggtg gcggcgcagt tcggaatcat ggcaatcggt
     6421 ttggaggcgg cgtgggtcgg ataatcgagg attttcacgt ccaacacggt ggtcagtccg
     6481 cccaagcctt gtgcgccgat gcccagcgcg ttgacttttt caaagagttc gaggcgcagg
     6541 gcttcggtgg tggacaattc cgcgccggac gcggcctttt cctgcaattc ttgaatgtcg
     6601 atgtggctca tcagggactc ttttgccatc agcacggctt tttcgggcgt gccgccgatg
     6661 ccgataccca agatgccggg aggacaccag cccgcgccca tggtcgggat ggttttcaat
     6721 acccaatcga cgatgttgtc ggaaggattg agcatggcga gtttggattt gttttcagag
     6781 ccgccgcctt ttgccgcgca ggttacttcg actttaccgc ccggcacgat gctcatatgg
     6841 atgacggcgg gggtgttgtc tttggtgttt tggcgtttgc cggccggatc ggcgaggacg
     6901 gaagcgcgca gcgtattgcc ttcccaagtg taggcgcggc gtacgccttc gttaaccatc
     6961 tcttccacgc tcatgtccgc atcccattgg acgttcatac cgactttgag gaagacggtt
     7021 gcgatacctg tgtcttggca gatggggcgg ttgttttccg cacacatacg gctgttgacc
     7081 aaaatctgcg tcatcgcgtc tttggcggca ggattttctt ccttctgcca cgccttataa
     7141 agcgcgtcga tgtagtcttt gggatgatag tagctgatga attggaaggc atcgcaaatg
     7201 ctttggataa agtcttcctg tttgatgacg gtcatgattg tgttcctttt caaggttgtg
     7261 gcggtaagtg tctgctgctg ttttaaacaa cctttttgaa ggctgttttt tatcaggttg
     7321 aaatagtgcc agcgtttgta cggtaaagac aatacataat tctaatctct tgattatcaa
     7381 tttgttttaa taaaatactg ttttactcgg attttgatat ttgtaatctt tgcctgttgc
     7441 tttgcggtag ggagaaatca gcaggcagtt cattgtaggc cgattgatga acaatgccgt
     7501 ctgaaagggc ttcagacggc attgtgttcg gacgggcggg cttattttgc ggcctgtccg
     7561 tcttcttttg caaaatattt ggcgtaaatc ttgtcgtatt cgccgctttc gcgtactttt
     7621 tccaacgcat cgttcagcat tttgacggtt gcttcgtcgc ctttgcgtac cgcgatgccg
     7681 tagtgttcgg tggtgaagtc gggcagggta acgaagtcca tccctttggc cggattgttt
     7741 ttcacataat tggcgatgac cgcgctgtcg ctgaccacgg aatccaagcc gccgttttcc
     7801 agttctttga taatcagggg aacgttttca aagcgcgcga ttttcggatt gtcgttgccc
     7861 aagagtttgg atacggagaa atcgcccgtg tagccggtta ccacgccgac tttgttcatg
     7921 tttttcaaat cttcggaaga agatactttt ttgcctttcg gaacgaggac gacttgggtg
     7981 atttcaaaat acgggtcgct gaagtccata gactgtttgc ggtcgtcggt aatggttacg
     8041 cccgacatca caacgtccgc atcgccgttg tttaaggcgg ggaaaaggct gtcccacggc
     8101 tggtgtttga attcgatttt aaaattgccc gccttcgcca tcgcgttcat caaatccaca
     8161 tcgaaacctt cgacattgcc tttcgagtct aaagattcaa agggggcaaa ctcggcgttg
     8221 gaagccacgc ggtacacttt gtcggggttg gcggcaggcg cggcggtatc tttgccctga
     8281 ccgccgcagg cagacagcgc gagcgcggaa caggcaaggg cggcggcaat ccattttttc
     8341 atattcatca aaattccttt cggtaatcaa aaagcctaaa aaatcagttt aacataaaat
     8401 taacgttctt tttcacggaa aaacgcataa gtaacgtcgt ggaacaaatc cgtatgcggg
     8461 ctgtcctcat agctcaattc tgccaaggcg tcctcaaaag caagctgaat cgactcgacc
     8521 gcgatttcgg cggtttcggc gggaagggcg cggattaagg ctttgaatgc ggtggacagg
     8581 acgtggttct gcatacgcag cacgtcgttc gcctcttcca gatattccaa acgctgttcg
     8641 atgctgccca tgatgtatcc tcgcgaatct gttaaaatgc gccattatat caaaccgcgc
     8701 tttgggcgcg ggtttgtctg cttgcaaaga ttaagaaccg gaagccgaaa atggatgcga
     8761 ataaagagcc gaattgggaa aaacgtttgg aaaacctgcg tcagacgcgc cgtatgaagg
     8821 cagtgccgac ttacgcgccg ctgtcccgcc cctctgaaac cggaacggac gcgcctgtgc
     8881 cgaaactgga cgatgagagc cgagcccgtg tgttgaaggc gtatctgaaa aaatggcagg
     8941 aggaacacaa tgccgccgtt gccgattgcg gcgaggaagt cgaaaccgac ccgatgatcg
     9001 tccttcagga aaactggctc aacgcgcagg cttccctgca aatgtcggtt tcggaaaaac
     9061 acatcgaatc gcggcggaga atccggtttg ataccaaaaa agacagtgtc gaatttgaaa
     9121 ccggacagat tgaaagcggg gaaaacgccg acgaggcaga atccggcacg aatgagccgg
     9181 cggtttcgga aaacggggaa aacgatgcgg aagaaacgtt gatgcccgtc caaatcaata
     9241 tcctgaaccc gaaagccgtc aaccggcgcg aagtgttctg cctgtccgaa caggagctga
     9301 ccgaacggct gatcaaacgc ctgcgcccgc atctgaccga taccgtcaac ggcatcatcc
     9361 gaacggcggt gcaaaagcag atggcggttt tcacctatca gctccagcag atgctgcacg
     9421 agcaggcggg ggcggtagtc gaagacgtgt tggaacacga tgtccgcaaa atcctcaacg
     9481 acatcaagta cgagttaaaa tacaaacgct gattgccatg ccgtctgaaa gccgtttcag
     9541 acggcatttg cattctcatc cgacttgaaa gcatcataga acaggcgcaa aatattaaca
     9601 aattaaacaa cgggcgcacg ctccgttgcc gaacggacaa aaccgaggaa tcaccgatga
     9661 gcaaaaaact ccatccgcaa acgctcgcca tacgcggcgg caaagaacaa accgaatacc
     9721 gcgaacacaa tcaggcattg tttctgacca gcagcttcat gtgggacaac gcccaacacg
     9781 ctgccgattt gttttcaaaa aaaatcaaag ggttcactta tacccgtacc gccaacccga
     9841 ccacagccgc ctttgaaaaa cgcatcgccg ctttggaagg tgcggaacgc gcggtcgcca
     9901 cttcggcggg tatgtctgcg attcaggcgg cgtttttcac ctttttgcag gcgggcgacc
     9961 atgtgatttc cagccgcagc ctgttcggca cgaccgtcgg ctttatcaat aacatcgttg
    10021 ccaaattcgg catcgaagtg agccacgtgt cgccgactga tataaacgaa tggaaagccg
    10081 ccgtcaaagc caataccaaa ctgctgtttt tggaaacgcc gtccaacccc ttaggcgaag
    10141 tggccgactt ggaagccttg gcggaattgg cgcacggcat cggcgcgctc ttggtggtgg
    10201 acaacagcct gctgtcgccc gtcggctcgc agcctttgaa acacggtgcg gatatttccg
    10261 tttcctccgc gaccaaagcc atagacggac acgggcgtgt gatgggcggc gtgttggcgg
    10321 gttcggaaga gttgttggcg caggtcgcca tgtattgcaa ctcttgcggg ctggcgatgt
    10381 cgccgtttaa tgcgtggcag ttgttgagcg gcgtggaaac cctgtcgctg cgtatggaaa
    10441 aacagttcga caacgccctg aaaatcgcac aatggctgca agcgcagccg caggttcaag
    10501 ccgtgtacta caccggattg tccgaccatc ctcaggcggc gcttatccgc aaacagcaaa
    10561 acggcggcgg catcgtcatc ggctttgaag ttgccgatca agaagcggcg tggaaagttg
    10621 tggacggcgt ggaactcttt tcccgcaccg ccaacctcgg tgacgtgcgc tcgaccatta
    10681 cccacccgtg gacaaccacg cacggcagga tgcagcccga agaaaaactc gccgccaaca
    10741 tccgccccgg attggtgcgc ctgtcggtcg gtttggaata tgttggcgat ttgattgacg
    10801 atttgaaaca ggcactcgca cgctgattgc gtgtttcaga cggcattgga cagcagatgc
    10861 cgtctgaaac ccttatctta tatggcatac acatcaacaa taggaaaaaa atatggatga
    10921 agcggttttt gccgattggg cgttgaaaat ctgcctgacc ggcttgatta tttttttggg
    10981 ttttatcgtt tggaatttgg gcaaggagtc caaagcaggc aaattcggca tcgccatttt
    11041 gtttttggtt ttgggcttgg gcgtgttcgg ttttgtattc aaagaactgc tgattaagtt
    11101 tttggtattg ccgaaatagg ctgccgcctg tcaaaatgct gtctgaaatc ataaaaagta
    11161 atctgcaccc taaaagtttg atttaaccgc caactgatta aggtgcggat ttttatgaca
    11221 a
//
LOCUS       DAWISC010000052        10919 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWISC010000052 DAWISC010000000
VERSION     DAWISC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.052"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..2099)
                     /locus_tag="WIW87_000970"
     CDS             complement(<1..2099)
                     /locus_tag="WIW87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ5174147.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSKVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVA
                     ETPDFNATADDLSALLQP"
     gene            2407..2937
                     /locus_tag="WIW87_000971"
     CDS             2407..2937
                     /locus_tag="WIW87_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ5174148.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            2940..3230
                     /locus_tag="WIW87_000972"
     CDS             2940..3230
                     /locus_tag="WIW87_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ5174149.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            3230..3508
                     /locus_tag="WIW87_000973"
     CDS             3230..3508
                     /locus_tag="WIW87_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ5174150.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            3557..4423
                     /locus_tag="WIW87_000974"
     CDS             3557..4423
                     /locus_tag="WIW87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5174151.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            4494..5252
                     /locus_tag="WIW87_000975"
     CDS             4494..5252
                     /locus_tag="WIW87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5174152.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            5325..5816
                     /locus_tag="WIW87_000976"
     CDS             5325..5816
                     /locus_tag="WIW87_000976"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ5174153.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            5847..6857
                     /gene="dusA"
                     /locus_tag="WIW87_000977"
     CDS             5847..6857
                     /gene="dusA"
                     /locus_tag="WIW87_000977"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ5174154.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(6927..7814)
                     /gene="gluQRS"
                     /locus_tag="WIW87_000978"
     CDS             complement(6927..7814)
                     /gene="gluQRS"
                     /locus_tag="WIW87_000978"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ5174155.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(7831..8028)
                     /locus_tag="WIW87_000979"
     CDS             complement(7831..8028)
                     /locus_tag="WIW87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174156.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            complement(8102..8461)
                     /locus_tag="WIW87_000980"
     CDS             complement(8102..8461)
                     /locus_tag="WIW87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ5174157.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            complement(8705..9760)
                     /gene="tal"
                     /locus_tag="WIW87_000981"
     CDS             complement(8705..9760)
                     /gene="tal"
                     /locus_tag="WIW87_000981"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ5174158.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
     gene            9857..10831
                     /locus_tag="WIW87_000982"
     CDS             9857..10831
                     /locus_tag="WIW87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ5174159.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
BASE COUNT         2397 a         2718 c         3178 g         2626 t
ORIGIN      
        1 ggttgaagta atgcggacaa atcgtctgcg gtggcgttga aatcgggtgt ttcggcaacg
       61 gtttccgtta cattttcctc aacggccggt gcttcagcag gttgcaacag tgcggacaaa
      121 tcatcggcaa cggtttccgc tgcattttcc tcaacggcag gtactttaga aggttgaagt
      181 aatgcggaca aatcgtctgc ggtggcgttg aagtcgggtg tttcggcaac ggtttccgtt
      241 atattttcct caacggacgg tgcttcggca ggttgaagca atgcggacaa atcgtctgcg
      301 gcggcgttga aatcgggcgt ttcaggcgca gtttccgcga cggcatcggt ttcgtacact
      361 ttcaggaaat cgtgcaactc ttccggtgtt tggacttcgg caactgtttt ttccaagatg
      421 gtttcgggcg aggaagcctt caggaagcct gccagtccgg agggtgaggc aggttttgcg
      481 gaagctgttt cttctgtgcc gatatggttg tttgagggca ggttgtcgga gaaatcggta
      541 tcgacggttt ccggtttgtt ttcggcagtt tgggcgacag attccggttc gggcgtgtcg
      601 atgacgattt cgacagggtt gtacgggttg aaggtctcgg gctcgtacac gctgtctgtg
      661 gattcgatgg cgttccaatc ggcatccgcg cgtttttggg tttcttcatc ctgcgtaagt
      721 gcgccggata aaatgccgtt ttgcgcggct gccaggctgt cgaaatccaa gtcgatgcgg
      781 ttggaaggcg tatcggtttc gacatcgaac gtttgttttg ccgataactc ttcttcagat
      841 tccccatcta aggcaagtgt gtcgtttaca tcgtttttcg gagcgggttc gggcgttgcc
      901 ggagtttcga cttcggcaaa ggtgatttct atgccgtcgt ctgccgcgtc gtcaaggtca
      961 ggctcttcct cagggacgga ttcttcggta cggcgcgcgc gtttggattg ggcaaggcgc
     1021 aaaagcagca gcagggcgat taatgccgcg cctccgccgg caagcagcaa ggtgtacgaa
     1081 ccgccgaaca gaccgtcaaa cagtccgctt tcggtttctt cttcggcaga aacctgttcg
     1141 acaggttcgg aaacggcgtt accggtttcg tcggtcggcg tgtcgatggc agaagcggcg
     1201 gcttcttggg gggcggattc ggcagcggtt tccgatgcgg cagtatttgc agcgggtaca
     1261 ggttcgggtc gaacggccgg tttttccgct tttgcttcgg gcgcggcaac ttttgcttca
     1321 ggtttttcaa ccggtttctc taccgttgcc tgtttggacg gttcggacgg catggatgcg
     1381 gtttcggctt tgggtttcgc cgtttgcggt ttgggttgtt ccgctttgat cctgttcaga
     1441 ttcggaatgt gaagcacgct gcccgcacgc agtctgccgt gtgcggaaac atttgggttt
     1501 gccttcagca gcgcatcggc aacctgttcg agcgtcaggt gtttcgggcg gatggcggcg
     1561 gcaatctgtt tgaccgtttc gcctttgcgg acggtatggg ttttgccgtt gtatgccggt
     1621 ttgacggctg cgttcgcgct gtctttttta tcggttttgc ggagggcttt ggcgttttga
     1681 ttttcttggg actctgctgt cggagcggtt ttgcggtgtg tcttgccgtc tgaaagtgca
     1741 gatttggttt tgggcgagta gccgacagga tcgaggatgg cggtgtattc gcgtacctgt
     1801 gcgcctgcgc cgatgcggaa caccaggacg ggatcgcgga ctgcctgttc ggaagaaacg
     1861 gcaatgacgg ctttgtcgcc caacttgtgg actttggcgg tcaggccttt ttcggaaacg
     1921 gtaacgctgc cgccgcctag cagggctttg gcttcttcgc cggttacggt aatgctgccg
     1981 gaaaagggtt cgtcaaggtt ggactggata ttcagtccgc ccagtccagc atgtgcctga
     2041 aaggatgcgg caactgcgac ggaggcggca atcagtttga tttgtctgtt gtttttcaag
     2101 atgtatcccc tgtgggttgg cggctgaata cggtttgacc gcgtacagtc tgtaaatttc
     2161 gtcatcatcg ggcatcggcg gggcagtcgg ccggcgggca tttaatatgt gaatgtaccg
     2221 accgccgcca cattttaaac ggcaatcatt cgccgttttt acaaattatg acatatctcc
     2281 atcttttttc aaaaacatct gtgcatattt gcatcaatca aaacaaaatt tgttggtttt
     2341 gcaggtgcaa aaacagggtt ctgcctgtat gattagcgtt tatttgattt gctttctcat
     2401 ttggatatga aattcgtcag cgaccttttg tccgtcatcc tgtttttcgc cacctatacc
     2461 gttaccaaaa acatgattgc cgcaacggcg gtcgcattgg ttgccggtgt ggttcaggcg
     2521 gcttttctgt attggaaata taaaaagctg gatacgatgc agtgggtcgg attggtgctg
     2581 attgtggtat tcggcggcgc aaccattgtt ttgggcgaca gccgcttcat tatgtggaag
     2641 ccgagcgttt tgttttggct gggcgcgctg ttcctgtggg gcagccacct cgccggtaaa
     2701 aacggcttga aggcgagtat cggcagggag attcagcttc cggatgccgt atgggcgaaa
     2761 ttgacgtata tgtgggtcgg tttcctgatt tttatgggta tcgccaactg gtttgtgttt
     2821 acccggttcg agtcgcaatg ggtcaactat aaaatgttcg gctcgactgc actgatgctt
     2881 gttttcttta ttattcaggg tatttatctg agtacctgtc tgaaaaagga ggattgactg
     2941 tggaatattt tatgttgctg gcaacagacg gggaggatgt gcacgaggcg cgtatggcgg
     3001 cacgtcccga acacctcaaa cggctggaga cgctgaagtc ggaaggccgg ctgttgacgg
     3061 caggcccgaa tcctttgccg gaggactcca accgcgtttc gggcagtttg attgtggcgc
     3121 agttcgagtc tttggatgcg gcgcaggctt gggcggaaga cgatccctat gttcatgcag
     3181 gcgtgtacag cgaagtgctg atcaagccgt ttaaagcggt gttcaaataa tgccggccgt
     3241 cgatttgatc cgcgaacgcc tgcagacgct cgatccgctg gtgttggaaa tcggcgatga
     3301 gagccatctg cacaaaggac acgcgggcaa taccggcggc ggacattatg ccgttttggt
     3361 cgttagcggc cgttttgaag gcgtaagccg cctgaaccgc cagaaaacgg tcaaatcgct
     3421 gctcaaagat ttgttttcag gcggcatgat tcacgcgctc ggcatccggg cggctacccc
     3481 tgacgagtat ttccatacgg cggactgaat gaagtctgcc cgaacatttc aatttaaaat
     3541 ttaaagagag aagattatga aagcaaaaat cctgacttcc gttgcactgc ttgcctgttc
     3601 cggcagcctg tttgcccaaa cgctggcaac cgtcaacggt cagaaaatcg acagttccgt
     3661 catcgatgcg caggttgccg cattccgtgc ggaaaacagc cgtgccgaag acacgccgca
     3721 actgcgccaa tccctgctgg aaaacgaagt ggtcaatacc gtggtcgcac aggaagtgaa
     3781 acgcctgaaa ctcgaccggt cggcagagtt taaaaatgcg cttgccaaat tgcgtgccga
     3841 agcgaaaaag tcgggcgacg acaagaaacc gtccttcaaa accgtttggc aggcggtaaa
     3901 atatggcttg aacggcgagg catacgcatt gcatatcgcc aaaacccaac cggtttccga
     3961 gcaggaagta aaagccgcat atgacaatat cagcggtttt tacaaaggta cgcaggaagt
     4021 ccagttgggc gaaatcctga ccgacaagga agaaaatgca aaaaaagcgg ttgccgactt
     4081 gaaggcgaaa aaaggtttcg atgccgtctt gaaacaatat tccctcaacg accgtaccaa
     4141 acagaccggt gcgccggtcg gatatgtgcc gctgaaagat ttggaacagg gtgttccgcc
     4201 gctttatcag gcaattaagg acttgaaaaa aggcgaattt acggcaacgc cgctgaaaaa
     4261 cggcgatttc tacggcgttt attatgtcaa cgacagccgc gaggtaaaag tgccttcttt
     4321 tgatgaaatg aaaggacaga ttgcgggcaa ccttcaggcg gaacggattg accgtgccgt
     4381 cggtgcactg ttgggcaagg caaacatcaa acctgcaaaa taattctgaa aacgggatat
     4441 ggcggcaaga cgttcagaca ggcgttttgc cgccgcgcag gacagggaat accatgaaac
     4501 agaaaaaaac cgctgccgca gttattgctg caatgttggc aggttttgcg gcagccaaag
     4561 cacccgaaat cgacccggct ttggtggata cgctggtggc gcagatcatg cagcaggcag
     4621 accggcatgc ggagcagtcc caaaaaccgg acgggcaggc aatccgaaac gatgccgtcc
     4681 gccggctaca aactttggaa gttttgaaaa acagggcatt gaaggaaggt ttggataagg
     4741 ataaggatgt ccaaaaccgc tttaaaatcg ccgaagcgtc tttttatgcc gaggagtacg
     4801 tccgttttct ggaacgttcg gaaacggttt ccgaagacga gctgcacaag ttttacgaac
     4861 agcaaatccg catgatcaaa ttgcagcagg tcagcttcgc aaccgaagag gaggcgcgtc
     4921 aggcgcagca gctcctgctc aaagggctgt cttttgaagg gctgatgaag cgttatccga
     4981 acgacgagca ggcttttgac ggtttcatta tggcgcagca gcttcccgag ccgctggctt
     5041 cgcagtttgc cgcgatgaat cggggcgacg ttacccgcga tccggtcaaa ttgggcgaac
     5101 gctattatct gttcaaactc agcgaggtcg ggaaaaaccc cgacgcgcag cctttcgagt
     5161 tggtcagaaa ccagttggag cagggtttga gacaggaaaa agcccgcttg aaaatcgatg
     5221 cccttttgga agaaaacggt gtcaaaccgt aatggcattt ccaataccga tgccgtctga
     5281 agcctttcag acggcattgc acgttcaggt aaggaggacg gcttatgcgt gcggtcatac
     5341 agaaaacggt aggtgcaaag gtggatgtcg tgtccgaagc cggcacggaa acctgtggca
     5401 aaatcgacgg cgggtttgtc gtgttactcg gcgtaacgca tagcgacaca gaaaaagatg
     5461 cacgctatat cgccgacaaa atcgcccatt tgcgcgtgtt tgaagacgaa gcgggcaagc
     5521 tgaacctgtc tttgaaagat gtcggcggcg cggtgctgct ggtgtcgcag tttacgcttt
     5581 atgccgacgc ggcaagcggg cggcggcctt cgttttccca agccgcacct gcagaacagg
     5641 cgcagcagct ttacctgcga acggcggaac tgttgcgcgg acacgggatt catgtcgaaa
     5701 cagggcgttt ccgcacgcat atgcaggtgt cgctctgcaa cgatgggccg gtaaccatac
     5761 tgctggactc tttcatgacg cggatttccc caaaaatgaa ggttgttccg gattgaaatt
     5821 gaatccgcaa tgataaaata tcgacaatga acgacaatac acacaccctt cccccgcgcc
     5881 acctgtccgt cgcccccatg ctcgactgga cggacaggca ctaccgttac cttgcccgcc
     5941 agattacccg aaatacttgg ctgtacagcg aaatggtcaa tgccggtgcg attgtttacg
     6001 gcgacaaaga ccgctttttg atgttcaacg aaggcgagca gcccgtcgcc ctgcaactgg
     6061 gcggcagcga tccgtccgat ttggcgaaag ccgccaaagc cgccgaggca tacggttaca
     6121 acgaggtcaa cctcaactgc ggctgcccca gtccgcgcgt gcagaaaggc tcgttcggcg
     6181 cgtgtctgat gaacgaagtc gggctggttg ccgactgcct caacgccatg caggatgcgg
     6241 tcaagattcc cgttaccgtc aaacaccgca tcggtgtgga caggcagacc gaataccaaa
     6301 ccgttgccga tttcgtcggc acgctgcgcg acaaaaccgc ctgcaaaacc tttatcgtcc
     6361 acgcccgcaa cgcttggctg gacggtcttt cccccaaaga aaaccgcgac gttcccccgt
     6421 tgaaatacga ttacgtttac cgcctcaagc aggagtttcc cgggctggaa atcatcatca
     6481 acggcggcat caccaccaac gaagcaatcg caggacacct gcaacacgtt gacggcgtga
     6541 tggtcgggcg cgaggcgtac cacaacccga tggtgatgcg cgaatgggac aggctgtttt
     6601 acggcgatac ccgcagcccg attgaatacg ccgatttggt gcagcgtctc tacacataca
     6661 gccaagccca aatccaagcc ggacgcggca caatcttgcg tcacatcgtc cgccacagcc
     6721 ttgggctgat gcacggtctg aaaggcgcgc ggacttggcg gcgtatgctt tccgacgcaa
     6781 cgctcttgaa agacaacgac ggcagcctga ttctcgaagc gtggaaagag gtcgaacggg
     6841 caaatatgcg cgaatagggc ggggctgtat gtgtgaaatg ccgtctgaag gcttcagacg
     6901 gcatttgtgc gtttgtcggg cggtgtttag ggggcggtaa cggcgtgttt cggcactttg
     6961 tccatatccc agtgtgccac cgcccagtcg agcagttcgg cagggcggtc ggtttccggt
     7021 gcttcgggca gcttgaggta acggaacact tggcggagga gttgttcgcg gcggtttaaa
     7081 tccaatgcgg gggcgagcgt ctgtttcgac catttctgcc cttgtgcgtt ggtcagcagc
     7141 ggcaggtggg catattgcgg tgtcggaacg tccaaacact gctgcaaata gatttggcgc
     7201 ggcgtggaaa cgagcaggtc ttgtccgcgg acgatgtggg taacgccctg ttcggcatcg
     7261 tcggcaacga cggcgagctg gtatgcccag taaccgtctg cacgaagcag gacgaaatcg
     7321 ccgatgtcgc gggcgaggtt ttgggcgtaa ccgccgacga tgccgtctga aaaaccgata
     7381 atgcggtcgg ggacgcggat gcgccacgcc ggctgtttgc cttgcagtgc agggcgttgg
     7441 ccggggtggc ggcaacgtcc gttatagacg aacccgtctg cgccccgcct tgccccggcc
     7501 tgccagtctt tgcggctgca atggcaggga tagaccagtc cggcggtttt caggcggcat
     7561 agggtttctt catacagggc gtaacggcgg ctctgatagg cgacttctcc gtcccactcg
     7621 aatccgaatg cctcaagcgt gtgcaggata tggcttgccg cccccggcat ttcgcgcggc
     7681 ggatcgaggt cttccatgcg gatcagccat ttgccgccgt gcgcgcgcgc atcggcatag
     7741 gaagcgacgg cggtcagcag cgagccgatg tggagcagcc cggtcgggct gggggcaaaa
     7801 cgtcctgtgt acatatctgg tacagcccct ttatttaaga ctattaatca aagccattat
     7861 ctcatcttta ttcagttcca tcccgggctc ttcaagcaag gttaaatcat atagggcatt
     7921 atattgctct tcggtagctg aaccatccat aagagcaggc gagaaaaaat caaaggctct
     7981 atctgcaatt ctctcattac ttgcatttct actaaccagt ttcgtcaatt ctgtatattt
     8041 tgaaaagttt atggaaaaat aaaacagcga aaaagttttg gtttcgctgt ttttgattta
     8101 attagcactg ataatcttca aattcccacg aaaaaaaacg aagtaaataa gtcaatgact
     8161 tttcccaagt ttcttttgaa cattctttaa gaattttctc aatttccgat ttaataacag
     8221 aatgattaaa ttcattcata atcatcatac ccgcccccca tttaaccctt tgattttgga
     8281 aacaattatg caaaatccat ttaggagagc atatgcgaac agaaaatata tctgcagcat
     8341 cactatcatc agttcctatg tctaaatcaa ttcccacaca aaaattgtct ttgatttcgg
     8401 gaacgaaatc ttcaaaggca caatcgtaaa gattgatggc tttcaattct aggttaatca
     8461 ttttatattc aatagtatgg ggaggtaccg gatccttaaa aatcagatct gaataaattt
     8521 cattgggtga aatgatttcg attgcttttg ccatgattct atttcctttt gtgttagtgg
     8581 gtaatgtcgt gcattaactt cttgcccatt aatattttta gggtgaatcc ttgatatgcc
     8641 gcactgtgtc cggtcaaacg ggcgatgccg tctgaaagcc tttcagacgg catcgggaaa
     8701 atgcctaagc caaaggcgcg agcagttttt caaacgcttc ttcaaactgt ttcaaaccgt
     8761 cttcctgcaa acgcgttgcc aaggtttcga catcgatgcc gagcgcggcg gtttcggcga
     8821 gctgcgcttg tgcttcttct acgccttcgg tcagcgtggc tttggctgtg ccgtggtcga
     8881 taaaggcttt gagcgtggca tcgggaacgg tgttgacggt gtgcgcgccg atcaggctgt
     8941 caacgtagag cgtgtcggga taggccgggt ttttcacgcc ggtagatgcc cataaaagct
     9001 gcacgcggtt tgcgcctttg gtttccagcg cggcaaattc ggggctgccg aagtattgcg
     9061 cccagtcttg gtaggcggct ttggcaaggg cgatggcgat tttgcctttg aggtggtcgg
     9121 gcagtgttgt gtccagcgcg ccgtccacac gggagatgaa gaagctggcg acaacttgga
     9181 tatgggcaac gctttgtccg gctgctaagc gtttggcgat gccgcgcgcg taggcggcgt
     9241 aggctttgag ggtttgggcg cgtgagaaca gcagggtcag gttcacgctg atgccgtctg
     9301 aaacgagggt ttcgagcgca tcgatgcctg cgtcggtggc aggcacttta atcatcgcgt
     9361 ttttgcaccc gatggcggcg tagaggcggc gcgcttcttc aaccgtgcct tgcgcgtctt
     9421 tggacaattc gggcgaaact tcgaggctga cgaagccggt tttgccgccg gtggattcgt
     9481 gttcggcaag gcaaacgtcg caggcggcac gcacatcggc aaccgccatt gtttcgtagc
     9541 gttgtttggg gctgaggttt tgctgcttga gggcggcgat ttcatcggcg taaagcgcgt
     9601 cgccggcgaa ggctttttgg aagatggcgg gattggaagt tacgccgcac acgccctgtt
     9661 tcaacatttg cgccaattcg ccgctttgca ctagcgagcg ggaaaggttg tccagccaga
     9721 tttgttgtcc taatgcttta acgtccgata aaatggtcat ctctgattcc tttggatgga
     9781 taggcggggt ttgagggctt atgctacccc gattcggaaa ttttgggtag ttttattaca
     9841 gcaaaggcgg atggcaatgg cagaaaacgg aaaatatctc gactgggcac gcgaagtgtt
     9901 gcacgccgaa gcggaaggct tgcgcgaaat tgcagcggaa ttggacaaaa acttcgtcct
     9961 tgcggcagac gcgttgttgc actgcaaggg cagggtcgtt atcacgggca tgggcaagtc
    10021 gggacatatc gggcgcaaaa tggcggcaac tatggcctcg accggcacgc ctgcgttttt
    10081 cgtccaccct gcggaagcgg cacacggcga tttgggtatg attgtggaca acgacgtggt
    10141 cgtcgcgatt tccaattccg gcgaaagcga cgaaatcgcc gccatcatcc ccgcactcaa
    10201 acgcaaagac atcacgcttg tctgcatcac cgcccgcccc gattcaacca tggcgcgcca
    10261 tgccgacatc cacatcacgg cgtcggtttc caaagaagcc tgcccgctgg ggcttgcccc
    10321 gaccaccagc accaccgccg tcatggcttt gggcgatgcg ttggcggtcg tcctgctgcg
    10381 cgcacgcgcg ttcacgcccg acgatttcgc cttgagccat cctgccggca gcctcggcaa
    10441 acgcctactt ttgcgcgttg ccgacattat gcacaaaggc ggcggcctgc ctgccgtccg
    10501 actcggcacg cccttgaaag aagccatcgt cagcatgagt gaaaaagggc tgggcatgtt
    10561 ggcggtaacg gacgggcaag gccgtctgaa aggcgtattc accgacggcg atttgcgccg
    10621 cctgtttcaa gaatgcgaca attttaccgg tctttcgata gacgaagtca tgcatacgca
    10681 tcctaaaacc atctccgccg aacgtctcgc caccgaagcc ctgaaagtca tgcaggcaaa
    10741 ccatgtgaac gggcttctgg ttaccgatgc agatggcgtg ctgatcggcg cgctgaatat
    10801 gcacgacctg ctggcggcac ggattgtata gtggattaac aaaaaccagt acggcgttgc
    10861 ctcgcctcag ctcaaagaga acgattctct aaggtgctta agcacgagtg aatcggttc
//
LOCUS       DAWISC010000053        10896 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWISC010000053 DAWISC010000000
VERSION     DAWISC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.053"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(74..457)
                     /locus_tag="WIW87_000983"
     CDS             complement(74..457)
                     /locus_tag="WIW87_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ5174160.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
     gene            complement(515..1891)
                     /gene="argH"
                     /locus_tag="WIW87_000984"
     CDS             complement(515..1891)
                     /gene="argH"
                     /locus_tag="WIW87_000984"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ5174161.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(1910..2779)
                     /gene="galU"
                     /locus_tag="WIW87_000985"
     CDS             complement(1910..2779)
                     /gene="galU"
                     /locus_tag="WIW87_000985"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ5174162.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="WIW87_000986"
     CDS             complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="WIW87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ5174163.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDTEVKNRESHRAQALAELLRKLAL"
     gene            complement(3399..3632)
                     /locus_tag="WIW87_000987"
     CDS             complement(3399..3632)
                     /locus_tag="WIW87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174164.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSATKENRYV"
     gene            complement(3740..4273)
                     /locus_tag="WIW87_000988"
     CDS             complement(3740..4273)
                     /locus_tag="WIW87_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ5174165.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(4374..4832)
                     /gene="nudB"
                     /locus_tag="WIW87_000989"
     CDS             complement(4374..4832)
                     /gene="nudB"
                     /locus_tag="WIW87_000989"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ5174166.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5043..5119
                     /locus_tag="WIW87_000990"
     tRNA            5043..5119
                     /locus_tag="WIW87_000990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5077..5079,aa:Pro,seq:ggg)
     gene            5277..7103
                     /gene="mafB"
                     /locus_tag="WIW87_000991"
     CDS             5277..7103
                     /gene="mafB"
                     /locus_tag="WIW87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ5174167.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNINSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            7106..7570
                     /locus_tag="WIW87_000992"
     CDS             7106..7570
                     /locus_tag="WIW87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174168.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            7759..7941
                     /locus_tag="WIW87_000993"
                     /pseudo
     CDS             7759..7941
                     /locus_tag="WIW87_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            7953..8240
                     /locus_tag="WIW87_000994"
     CDS             7953..8240
                     /locus_tag="WIW87_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ5174169.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            8736..9992
                     /locus_tag="WIW87_000995"
     CDS             8736..9992
                     /locus_tag="WIW87_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ5174170.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            10023..10442
                     /locus_tag="WIW87_000996"
     CDS             10023..10442
                     /locus_tag="WIW87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174171.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            10633..10857
                     /locus_tag="WIW87_000997"
     CDS             10633..10857
                     /locus_tag="WIW87_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ5174172.1"
                     /translation="MRLATKDLAEAIRKGQVRKSSFNTEQLRAIEKGESKIPDYTWHH
                     HQDTGRMQLIREGLHHDTGHIGWEAMNKGR"
BASE COUNT         2844 a         2363 c         2923 g         2766 t
ORIGIN      
        1 cgtctgaaca gggttgctgg aatagtattg ccatcccagc agatacagtt tgtcggggtc
       61 ttgccaatat tgttcatcca gactgttcag cagtgaggcg gttttgttgt cgagatgtta
      121 tatcccacat ttctttcagg tttttacctt ccgattggag gcggcggatt tctttgtcca
      181 atgcgtctgc cgcccgatgg atcagcattg cgctgtgtac cgcgccgatt tttttcagaa
      241 cggaacaagt cttttcggcg gcggggaaac cccagttgca gacaaattgc agtatgctgc
      301 cgttaccgat atcggcttct gtccgccaaa tcaaaaccag ctcctgttcc cgcccgtcca
      361 tgctttcgag tttgccgtca tgctgttcaa aaagtttgtc caccgcctgc cacatcatct
      421 gttcgaagcg gtcggcttgg gtatccgtat cggtcatcgt gttcttgcct tttaaaaatg
      481 ccgtctgaac atttcttcag acggcatttg ggggttaagc caacatttcc cgccagcgtt
      541 tcacttggaa gcggacttgt tccggcgcgg taccgcccaa gtggttgcgg gcgtttaagc
      601 tgccttcggg tgtcagcacg ccgtaaacgt cgtcggcaat caaatcgctg aaaccttgta
      661 agacttcgag cggcagttcg ctcaaatcga cgcccgcttg gtcggcgtgg cgcacggctt
      721 gggcgacgac ttcgtgggca tcgcggaaag gcatgccttt tttgaccaga taatccgcca
      781 agtcggtggc ggtagcgaag ccctgcatca cggcggcgcg catattgtcg ggtttgacgg
      841 ttacgccgcg catcatatcg gcgtaaatcc gcaacgtgtc gataagcgtg tcggcggtgt
      901 cgaacaaggg ttctttgtct tcctgattgt ctttgttgta cgccaagggt tgggatttca
      961 tcagggtaat cagaccgata aggtgtccga tgacgcggcc ggatttgccg cgcacgagtt
     1021 cgggcacgtc ggggtttttc ttctgcggca tgatggacga acctgtgcag aaacggtcgg
     1081 cgatgtcgat aaagccgaaa cgcgggctca tccacaaaat caattcttca gacaggcggc
     1141 tcaggtgaac cataaccagc gaggcggcgg ctgtgaactc aatggcgaaa tcgcggtcgg
     1201 atacggcatc gagcgagttc tggcagattt gttcaaagcc caatagctcg gcggtgattt
     1261 cgcgctgaat cgggtaggtc gtcccggcaa gggcggctgc gccgagcggc atacggttga
     1321 cgcggcagcg gcagtccgcc atccgttcgt tatcgcgtcc gagcatttcg acgtaggcga
     1381 gcatatggtg tccgaagctg acgggctggg cgacttgcag gtgggtaaag cctggcatga
     1441 cggtttcggc gttttgttcc gccaaatcca gcaatgccgt ctgaaggctt tgaatcaggc
     1501 tttgtataac ggtaatctgg tcgcgcagcc acaggcggat gtcggtggcg acttggtcgt
     1561 tgcggctgcg gccggtgtgc aggcgtttgc ccgcgtcgcc gattttgtcg gtcaggcggc
     1621 gttcgatgtt catatggaca tcttccaaat cggacgacca ttcgattttg ccgctgcgga
     1681 tttcttcgag gatttccgcc ataccccggc ggatgtccgc caaatcgcct tcgtccaaca
     1741 cgccggtttc tttcagcatt tgcgcgtgtg ccagcgagcc ttggatgtcc cattcggcaa
     1801 gccgtcggtc gaaaccgatg gaggcggtgt attgtttgac gagttcggaa acgggttcgt
     1861 tgaaacgtcc ggaccaggtt ttgtcgtgca taaggattcc ttgatggggt tattcggtgc
     1921 ggtatttttc caaaagccgg cggaagggtt cgccggtttc gggatgtttc agaccgtagg
     1981 cgacggtggc ttcgaggtag cccagtttgc tgccgcagtc gtagcgcgta ccttcaaagg
     2041 ggtgcgccag gacaaattcg tgatcgagca gcttggcgat gccgtctgta agctggattt
     2101 cgttgcccgc gccgcgcgga agattggtta agaggtcgaa aatgcgcggg gtgaggatgt
     2161 agcgtccaac aacggcaagg ttggagggcg cgtcttcggg cttgggtttt tcgacaatgc
     2221 cggtaatgcg ttggaactgt ttgagctgtt cggtttcgac gatgccgtat gagccggttt
     2281 gcgatgcttc aacggtttct acgcccaaaa tgctgttgcc gctgcgcccg tacacttcga
     2341 ccatttgttt gagcgcgcct ttgggggcat caatcaggtc gtcggcaagg ataacggcaa
     2401 agggttcgtc tccgatggcg gcgcgggcgc acaagacggc gtgtcccaag cccagtgctt
     2461 ccgcctgacg gatgtagagg caggtaatgt tcggcggcag gatgttgcgg acgtgttcca
     2521 acaatttgtc tttatggcgc atttccaact cggtttcgag ttcgtatgcc ttgtcgaaat
     2581 ggtcttcgat gctgcgtttg ttgcgtccgg taacaaacac catttccgtg cagccggctt
     2641 ccacggcttc ttctacggcg tattggatca gcggcttgtc gacgatgggc agcatttctt
     2701 tcgggctggc cttggtggcg ggcaggaagc gggttcccat ccctgcgacg gggaaaacgg
     2761 ctttccgtat gggtttcatt ctttttcctt tgtattgttt tgatgtttaa agggcgagtt
     2821 tgcgtaagag ttcggcaagt gcctgcgcgc ggtggctttc gcggtttttg acctccgtat
     2881 ccaattcggc ggcggttttg ccgtgttcgg gcagataaaa atacgggtcg taaccgaaac
     2941 cgttttgccc gagcggcgtg tcgttccact gcccgtgcca tacgccctcg gcgataatcg
     3001 ggcgcgggtc gtctttatgg cggacaaaaa ccaatacgca gacatagcag cagcttttgt
     3061 ctgccttgcc gacaagttcg gcggcaagtt tcaggttgtt ggcggtatcg gatttgggat
     3121 tgtcgcccgc gtaacgtgcg gaatggatgc ccggcgcgcc gtttaaggcg gcggcacaga
     3181 tgccgctgtc gtcggcgagt gcgggcagcc cgctgtattt ggcggcatgg cgtgcttttg
     3241 ccagcgcgtt ttcgacaaag gtgggatagg gttcggggca ttcgggtatg ccgaatgcgg
     3301 attgcggcaa tacggtgatg ctgtaaggtt tgaataagtt gccgaactct tcgagcttgc
     3361 cggcattgcc gcttgccaaa acgatttttt ccggtttttc agacatagcg gttttccttt
     3421 gtggcggatt gggcggcgcg tagggatttg tgccgcaggt agagggcgag gctgccgatt
     3481 tgtccgaaca gtgcgccgaa tgcgaaaaga aaggaggcga cggcgaaggc tttgctgccg
     3541 acggtcagca gataagcacc caagaggacg agcagcataa atgccagtgt gaagagggca
     3601 acagacaaca gatagatttt tcggcggttc atggcgttcg gtcggaaacg gtatgttcgg
     3661 attatagccg attgggacgg tattccctag agcttggaaa aatgccgtct gaaacggcgt
     3721 tcagacggca tggcgggcgt tatgcctgtt tgttccaacg ttcgatggat tctttgatga
     3781 cttttttcgc ttcttccgca tcgccccacg attcgacttt ggtcgtacct gcttttttca
     3841 ggtctttgta atggttgaag tggaactcga tttgtttgat gagctgttgc ggcaaatcgg
     3901 acaaagtttt gtaggcgttg ccgttgttgc ggtcgtcggc aggaacgcag acgattttgt
     3961 cgtccacttc gccgtcgtca acgaatttca tcacgccgat aacgcgcgct tccaagaata
     4021 cgccggttgc caaaggttgt tcggtaacga gcagcacgtc caattcgtcg ccgtcttcgt
     4081 ccaaagtttg gggaatgaag ccgtagttgg tcggtttggc gaagatggcg ggttcgacgc
     4141 ggtcgagttg gaatgcggcc agtttgcggt tccattcgat tttgtggttg ctgccggcgg
     4201 ggatttcgtt gacaacgttg atgatgccgc cgtccacgtc gccgggggtc aggatttggt
     4261 tgaagtctgc catttggttt cctttgtttg ggaaagtgtg aagtttgaaa gtatagcaca
     4321 aacgtccggc tgaaaatgcg cccgatgcct ctgaaagggt gtacgggcgc gtgttaccgt
     4381 ttgcccaaaa acctgcccag ttccaaaatc gcgcgcctgt tggacgggga aaatactttt
     4441 tccgctgctt cttccatatc gaaccaaccg taggagacgt gttcttcggg ttgcagggcg
     4501 atgggcgtat cacgcgggat ttcggcagag aagaggtgtt cgcggttttc aaacacgcct
     4561 tttggatagc ggtgccgcca gtggtggtag atttcgtaaa ccgtgctgtc gtgccagtct
     4621 tgaagctgcc cgtccgccag caggatgccg gtttcttccc aaacttcgcg ccttgccgtt
     4681 tgggcgacgg tttcgcccgg ttcgaggctg ccggttaccg actgccaaaa tccttccgga
     4741 tgcgtgcgtt cgatgagcag gatgccgccg tccccgctat aaaggacgac cagtgcggaa
     4801 acggggtatt tgagcggttt tgccatcggc atctttcggc gggctgcggt aatgaagggg
     4861 cggattatag caaacgccgc acgttatggc gtttatcctt ttccgtatcc ttttttctgc
     4921 acggatggga cgcgccggtg tttgccggta aattttccgt tgtgtcaaaa agataagggc
     4981 ggttgtgatt ttaatgcttg ccaaagcgtc gggcggaaac tataatccga aacttatcga
     5041 gtcggagtgt ggcgcagtct ggtagcgcac ttgcatgggg tgcaaggggt cgaaggttcg
     5101 aatcctttca ctccgaccaa aaattccgaa agccgctttc aaaagcggct tttttgccgt
     5161 ccgtatgatt atgatgtaga gtacgcggcg acagacattc aaatgccgtc tgaaaaccgt
     5221 tcagatggca tctctttatc ttagtttcat tccgtaccat cttaaggaac atcaaattgg
     5281 gcatttcccg caaaatatcc cttattctgt ccatactggc agtgtgcctg ccgatgcatg
     5341 cacacgcctc agatttggca aacgattctt ttatccggca ggttctcgac cgtcagcatt
     5401 tcgaacccga cgggaaatac cacctattcg gcagcagggg ggaacttgcc gagcgcagcg
     5461 gccatatcgg attgggaaaa atacaaagcc atcagttggg caacctgatg attcaacagg
     5521 cggccattaa aggaaatatc ggctacattg tccgcttttc cgatcacggg cacgaagtcc
     5581 attccccctt cgacaaccat gcctcacatt ccgattctga tgaagccggt agtcccgttg
     5641 acggatttag cctttaccgc atccattggg acggatacga acaccatccc gccgacggct
     5701 atgacgggcc acagggcggc ggctatcccg ctcccaaagg cgcgagggat atatacagct
     5761 acgacataaa aggcgttgcc caaaatatcc gcctcaacct gaccgacaac cgcagcaccg
     5821 gacaacggct tgccgaccgt ttccacaatg ccggtagtat gctgacgcaa ggagtaggcg
     5881 acggattcaa acgcgccacc cgatacagcc ccgagctgga cagatcgggc aatgccgccg
     5941 aagccttcaa cggcactgca gatatcgtta aaaacatcat cggcgcggca ggagaaattg
     6001 tcggcgcagg cgatgccgtg cagggcataa gcgaaggctc aaacattgct gtcatgcacg
     6061 gcttgggtct gctttccacc gaaaacaaga tggcgcgcat caacgatttg gcagatatgg
     6121 cgcaactcaa agactatgcc gcagcagcca tccgcgattg ggcagtccaa aaccccaatg
     6181 ccgcacaagg catagaagcc gtcagcaata tctttatggc agccatcccc atcaaaggga
     6241 ttggagctgt tcggggaaaa tacggcttgg gcggcatcac ggcacatcct atcaagcggt
     6301 cgcagatggg cgcgatcgca ttgccgaaag ggaaatccgc cgtcagcgac aattttgccg
     6361 atgcggcata cgccaaatac ccgtcccctt accattcccg aaatatccgt tcaaacttgg
     6421 agcagcgtta cggcaaagaa aacatcacct cctcaaccgt gccgccgtca aacggcaaaa
     6481 atgtcaaact ggcagaccaa cgccacccga agacaggcgt accgtttgac ggtaaagggt
     6541 ttccgaattt tgagaagcac gtgaaatatg atacgaagct cgatattcaa gaattatcgg
     6601 ggggcggtat acctaaggct aagcctgtgt ttgatgcgaa accgagatgg gaggttgata
     6661 ggaagcttaa taaattgaca actcgtgagc aggtggagaa aaatgttcag gaaataagga
     6721 acggtaatat aaacagtaac tttagccaac atgctcaact agagagggaa attaataaac
     6781 taaaatctgc cgatgaaatt aattttgcag atggaatggg aaaatttacc gatagcatga
     6841 atgacaaggc ttttagtagg cttgtgaaat cagttaaaga gaatggcttc acaaatccag
     6901 ttgtggagta cgttgaaata aatggaaaag catatatcgt aagaggaaat aatagggttt
     6961 ttgctgcaga ataccttggc aggatacatg aattaaaatt taaaaaagtt gactttcctg
     7021 ttcctaatac tagttggaaa aatcctactg atgtcttgaa tgaatcaggt aatgttaaga
     7081 gacctcgtta taggagtaaa taaaaatggc aatttggaat tatcggtact accttatccc
     7141 atcagctgct atcagaaata aatttaatgc aaataaagat atcatacttg atgagtatcg
     7201 atctaatggt tttcagaatt ttaatgagaa taaaagtttt gaaaattact ttatcgataa
     7261 tgatgttata ttattatcaa taataaatga agcaaaaaaa cagcttaaat tgaaagaatc
     7321 ttgggataaa gacgcaatca tgttttgtga taattttggt aatagtctta ccgtttggcc
     7381 agatgatata gagtgcgaac ttgatttaag atttgattat actaaattta ttcagaaaac
     7441 cattgattgg gcaataaaat ataattgtct acttgtaata gaaaaaacag gaaatgtagt
     7501 ttcccctaat ataaataatc tgatgtatga aataaaagca tatttggaaa gcaagccgtg
     7561 gcccatatga aacctaaact caacaagtag gatgtgtgcg gaacgcacgt atgcggttct
     7621 caaggtttga gctaagaggc cgtctgaaaa cagaaaaact gtttcagacg acctttcttt
     7681 taaccagttg ccacagcaac cggacaaaag cagcctacct ccacatccat ataggcaata
     7741 caggggagat attttgtaaa ttctacgaat attttacctg ctaaacaggg taggatatgg
     7801 tatgaagcga acattggctt aataaacact atgtcaagat cgaatcaggc tggtactaga
     7861 ttgttgtatt ccaattatgg attgctatat ataacaactg atcattatat ctctgcaaca
     7921 cggtttgtag cttggaaata ggagtataac ttatgcaatt agagattatc ggtagtaaaa
     7981 tttatacgga acaagatttt cataatcaaa tttcaaaaat attttctata caagattatt
     8041 atgggaacaa tcttgatgct ttatgggatt tattaagcac aaatgtagaa cgaccgatta
     8101 ctttggtatg gaaagatgct atgttctcaa aaaatcaatt agaaaatata tttattgaaa
     8161 tcgtaaatgt tctagaaaga gttaagaaac aagatgagga ttatggattc gaagaaaaat
     8221 ttaattatat tttagagtaa gtaaacccta attacattac gtacacaggc ttaaaactcc
     8281 ccagagccaa ttaagcaagc cgtaacccat ataaaactta aactcaacaa gtagcatgtg
     8341 tgcggaacgt acgcatgcgg ttcttaaagt ttgagctaag aggccgtcta aaaacagaaa
     8401 aaccgtttca gacggtcttt gtttaacgcc accgatccag cgggttacaa agcgcagtca
     8461 atgccgctgc gccttatgcc tccgaagcaa taggcagaac atttggacac ggtgaaaaca
     8521 aaaacgaaac cgcccaagcc gtcggacatt tccttttagg agcagctatt gcccgcgtca
     8581 acggtggtaa ttttgctgcc ggcggctcgg cagcagttgc agctgaaaag gcggcggaac
     8641 atcttgccca acagtataac gacggtaaaa ccgcaatcga tccgcaaaca ggcgagttca
     8701 atgccaacct gctgccggaa catatcaaag aggaaatcaa atcaaagagc ggggtgattg
     8761 catcgctgac gggcgcggcc gtgggcggca cgccggtaga tgcgcaaacc ggaggtgcgg
     8821 tcggacagaa tgcggtggaa aacaacctct atctgacatc ggaagcctta aagaaggaca
     8881 agcagacagc tcgtaaaatt tattccgtca taaaagagca agtcaagcat gaatgcagtt
     8941 ccacaggaag aattaccgaa tgtcgtcaaa atataggacg cattatcgaa tttacccaag
     9001 acaaacgctt tgacagtagg tttaaggact taaaaaaaga atccttatat tacctaaata
     9061 aacatcctga tttagtagcc tcttatttga aggctgaata cgaaaagctg gatagggaag
     9121 acaaaagtat cctgcaccgc tacatctcac ccggggctga aatcgtttcg ggcagtttgg
     9181 gggttgttct ttcaggagta gccggaggcg gatcttgtgc cgagactttc ggcttaggct
     9241 gtgccgccgc tttggttggt gtaacgtctt cctacgatca tgtcattact gggacgaaaa
     9301 acttcggaaa aaaagccagc gagcaacgac cgacgattgc ggttcaggcc ttgaagcagt
     9361 tggggctgtc ggagcaggct gcggaatatg ttcagttctc tatagatttg ttcagtgtgg
     9421 gtaaatcggg gggcggtata cctaaggcta agcctgtgtt tgatgcgaaa ccgagatggg
     9481 aggttgatag gaagcttaat aaattgacaa ctcgtgagca ggtggagaaa aatgttcagg
     9541 aaacgagaag aaggagtcag agtagtcagt ttaaagccca tgcgcaacga gaatgggaaa
     9601 ataaaacagg gttagatttt aatcatttta taggtggtga tatcaataag aaaggcacag
     9661 taacaggagg gcatagtcta acccgtggtg atgtacgggt gatacaacaa acctcggcac
     9721 ctgataaaca tggggtttat caagcgacag tggaaattaa aaagcctgat ggaagttggg
     9781 aggtgaaaac gaaaaaaggt gggaaagtga tgaccaagca caccatgttc ccaaaagatt
     9841 gggatgaggc tagaattagg gctgaagtta cttcggcttg ggaaagtaga ataatgctta
     9901 aggataataa atggcagggt acaagtaaat cgggtattaa aatagaagga tttaccgaac
     9961 ctaatagaac agcatatccc atttatgaat agtaatattt atgaaaaatt aggagattaa
    10021 tgatgaaaag aattaagtgc ttttgtgata aatttccatc aggagataca tttagaatgt
    10081 gtatcattct ggatgactat gataataggg ttgattatta tgtaggaata tatgattaca
    10141 ttacgtctac cttaatgagc gatatttact atcgatccac gattgatgag catttcaaga
    10201 ttatagaatt aatagaaaat aatccaaatg aaatttatga tgatggcggt ggtcaacaat
    10261 tttgcctaga atttcatcat gataaggtca ttttttacca caatgaattt gatgaagaag
    10321 atggttatcc agtattaagc tgttcgctgc atacttttaa aactgcttta attgcttgga
    10381 atgctttttt gcaattgcct aaaagtattc attcggtggt ggagactgtg attgaggaat
    10441 aagcataatt agcttaatga atagaatcag cgatatagat tggactgcaa atccacgctt
    10501 atacgctgtg ccatgattaa gatgttagaa cttgtattga atacaagttc tcataaacga
    10561 atggcagtaa gcatttgatt tagataaaat ccttgaatta gaataatcag gtctaagagc
    10621 tcgacaggac aaatgaggct ggcaaccaag gatttggcgg aagccattag gaaaggacag
    10681 gttcgcaaat caagctttaa cacagaacaa ttaagggcaa ttgaaaaagg agaatctaaa
    10741 ataccggatt acacttggca tcatcatcaa gatacaggaa ggatgcaatt gattcgtgaa
    10801 ggcttgcatc atgataccgg ccatattggt tgggaagcaa tgaacaaagg aaggtaacta
    10861 tgtggaaaat cataaaagag gatagtgatg atttag
//
LOCUS       DAWISC010000054        10878 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWISC010000054 DAWISC010000000
VERSION     DAWISC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.054"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(119..1336)
                     /locus_tag="WIW87_000998"
     CDS             complement(119..1336)
                     /locus_tag="WIW87_000998"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ5174173.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WIW87_000999"
     CDS             complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WIW87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ5174174.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMLREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2346..2810)
                     /locus_tag="WIW87_001000"
     CDS             complement(2346..2810)
                     /locus_tag="WIW87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ5174175.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3027..3803
                     /locus_tag="WIW87_001001"
     CDS             3027..3803
                     /locus_tag="WIW87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ5174176.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3941..4468
                     /locus_tag="WIW87_001002"
     CDS             3941..4468
                     /locus_tag="WIW87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174177.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4822..5676
                     /locus_tag="WIW87_001003"
     CDS             4822..5676
                     /locus_tag="WIW87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ5174178.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5717..6925
                     /gene="pncB"
                     /locus_tag="WIW87_001004"
     CDS             5717..6925
                     /gene="pncB"
                     /locus_tag="WIW87_001004"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ5174179.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPGIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7017..8735
                     /gene="argS"
                     /locus_tag="WIW87_001005"
     CDS             7017..8735
                     /gene="argS"
                     /locus_tag="WIW87_001005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ5174180.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9289..10707
                     /locus_tag="WIW87_001006"
     CDS             9289..10707
                     /locus_tag="WIW87_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ5174181.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2614 a         3066 c         2679 g         2519 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcatacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcgacgc caggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cactcgtcgg
      841 ctttgagcgc ggcggcaagc gcgacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgccggcgg
      961 taaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtagctaag acgacgtcca
     1141 gctcgcgcgg atcgggatgc tcttgcattt cgtgcgccag tgcgaccagt cggttggttt
     1201 cgccgctcat ggcggatacg acgactacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc tacgtttttg atgcgttcgg gcgagcctac tgatgtgccg ccgtatttat
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgggggtt gtcgggcagc ttggtttgct
     1381 ggaaaaaggg ttattattac tattttttac atggaattca agaacggact gcgctttccc
     1441 gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt cagattgttg acaaaatgcc
     1501 gtctgaacgg ttttcagacg gcatccggac gacaatcagg cggcggacaa cgcattttgc
     1561 tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg caaccagttt gcccaattcg
     1621 tccaaactga acggcgcgtc ttccgccgtc ccctgtattt cgatgatttt tcccgatgcg
     1681 gtcatgacga tattcacatc actgtcgcaa ccggagtctt cgggataatc caaatccaaa
     1741 agcggcacgc cgttcactac gcctactgac acagcggcaa cggcttcgcg gatggggttt
     1801 tcactcaaaa tgccgtctga aaccagtttg ccgacggcga tttgcagcgc gacaaacgca
     1861 ccggtaatcg aagccgtgcg cgtaccgccg tctgcctgaa tcacatcgca gtcaatcaag
     1921 atttgtcgtt caccgagttt ttccatatcc acgaccgcgc gcagggaacg cccgatcaaa
     1981 cgttggattt cttgtgtgcg cccggactgt ttgcccgccg aagcttcgcg gagcatccgg
     2041 gaagcagttg aggcaggcag catcccgtat tccgccgtta cccagccttg gtttttaccg
     2101 cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac aaatcacttt ggtattgccg
     2161 cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat gaggggtgat tttgatatcg
     2221 cgcaggctgt cggcggcgcg cgagatgcgg atgtaatcag gcatactgcc ctcccgttaa
     2281 aaacagataa attaaaaagc cttaaatatg aaaaatcaca tttaaggcct tcaaactgaa
     2341 aatttctacg cctcttcggc tttgctgcgg ataatcaaaa gcggcaggtg gctttggcgc
     2401 attaccgttt cggcaaaact gcccattaaa aggtgcatca gcccggtacg tccgtgcgta
     2461 cccaacacca gcaggtcggc accgttttca tcggcataat caaccaaatc ctgcgccatt
     2521 tcacgcgcac ccttattggc aaccagcagg tgtttgacgg tattttccac acccagttcc
     2581 tgggcggtgc gctcggcggc atccaaaact tcgttgcctt gcgcgacggc ggcggcttcg
     2641 tagctttcgt gttgcaaaaa ttcgggggcg agtgccatat attcggcagg attggcaacg
     2701 tgcaccaaag tcaggcgcgc accgttgacc ccggcaagct cggcggcatg tttcagggca
     2761 ttgatggacg tttcactgcc gtcaacggca acaaccaaat gtttgtacat atcgtattct
     2821 ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc gcagggacag tttgcgctgt
     2881 ttcattatag acccgccgtc gggctttata caacagccga acagcccgac cgctttccag
     2941 tataatatgc cgcttccgtg cagtcaggca ttttttgccg gctttcgttc actttttgat
     3001 ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc tttgacctct tcgcgacgct
     3061 tcggcggcat cgccagactc tacggagact ctgccttggc gcacttttca caggcacacg
     3121 tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcggt cgaggctttg gcgcggacgg
     3181 gcatcggacg tttgactttg attgatttgg acaacgttgc cgaatcgaat gtcaaccgcc
     3241 agctgcacgc cctgaccggc gacttcggca aagcaaaagt taccgccttg cgcgaacgca
     3301 ttacacaaat taatccgcaa tgcgaagtgt ttgaaattga agatttcgtt accgaagaca
     3361 atttgccgga atacttcgga aaaggttttg atttcgtcat cgacgcgatc gaccaagtgc
     3421 gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa acaaccgttt gtcctcagcg
     3481 gcggcgcggg cggacaaaaa aatccggcgt taatccaaac cgccgatttg agccgcgtaa
     3541 cccacgaccc gctgcttgcc aacctgcgct acaccttgcg gaaacgctac ggattcagcc
     3601 gcgatacgaa agcaaatatg cgcgtgcctt gcgtgtattc gaccgaaaat atcgtgccgc
     3661 cgcagtctag ggaggcttgt tcggcagatg ccgctccgca aggcttgtcg tgcgccggct
     3721 acggtgcaag catgctcgtt accgcttcgt tcgggctata ttgcgcacag gcggcggtgg
     3781 aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac aggattcaga cggcatttga
     3841 acaaactatg gttatgattt aagacaacaa aggatacgga taaaaaataa cataaaatat
     3901 atgattccta ataatatacc aagtatcgga gagctattta atggaattcg ttaataattt
     3961 agttattttt tcatttttat tactaatgct tattccgata ttttttgtag tatatggtat
     4021 ataccataag atacgttatc gcaaaatatg tatcctaaga acaagtttta tattattagt
     4081 ggtaatactt tgcagtatgt attacatata ttgccgttat cttgaccaac aaaaagtagc
     4141 ttattattgc atagatgaac aatgtatttc tattgttcat ctatacaaag attatggtat
     4201 aaactctccc acatatgcga gaatttacgc aggaaaaata ttgtttagat ttcaagtaag
     4261 agctaaaaat tacgctgaat tacttatgga agatgatata tcaattagta aaaaaatttt
     4321 ggggaataaa tttatcattt atgggtcgct acctgtaata tacggtaatg tagataatat
     4381 tgaagtaaaa gaagctactg gttatataga tagatccagt actgattata ttgtctcaag
     4441 aaacttaaaa ttcagacatt tatattaatt aagaggtttt agcaagagtg ccgtcaaaat
     4501 atagggcgca tcatcgaatt cgcgaaagac aaacgctacg atgaacgttt caaggatttg
     4561 aaaaaagaat ccataggcta tctgaaccgg catcccggtt tggtgtccga ctacctgaag
     4621 gcggcaatca agctgtcggt tcagaaaaac caacatcagc acgcctaaaa ccgtattcac
     4681 aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt ctgaacatct
     4741 gcccgaccac attatacgtg aatgtcggca gattgttttc ttttgtaaac ttatattaaa
     4801 atccacttac cgattcacgc catgccgccc atccctgccc catctgcacc atccgagcac
     4861 actgtcgcat gggtattcgg ccaacccgtt accgatttgc cccaggattt gtttattccg
     4921 cccgatgcat tgaaagtcgt attgggcagc ttccaaggcc ctttggatct actgctgtat
     4981 ctgatccgca aacagaatat cgacgtactg gatattccga tggtgaagat taccgagcag
     5041 tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc ggaatatctt
     5101 ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg taccgaaacc
     5161 gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgct ggcttacgaa
     5221 cagatgaagc tggcggcgca gggtttggac gcgctgcccc gagccggacg ggatttcgcg
     5281 tgggcttacc tgccgctgga aattgccgtc gaagccaagc tgcccgaagt ctatattacc
     5341 gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacacacgcg cagccacgaa
     5401 gtaatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg ccgtttgaac
     5461 ggacacggaa tatgcaggtt tcacgacctg ttcaatccca aacagggcgc ggcttacgtg
     5521 gtcgtcaact tcatcgcact gttggagctt gccaaagaag gattggtcag aatcgtgcag
     5581 gaagacggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcgca ttcagacggc
     5641 atttccggca cacgaggcgg gcgcgatgtg ttctaatacg ccccaagccg ccaccaaaaa
     5701 tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg acctctacaa
     5761 attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc tttacgaatt
     5821 ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcagggaag acttggaagc
     5881 cgaactcgac gcgctctgcc aactacgctt cacccacgac gaactcggct atctgcgctc
     5941 cctgcgtttc attaaaagcg actttgtcga ttatctcgaa ctcttccagc tccaacgccg
     6001 ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgcatcg aaggtccgat
     6061 gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac tttacttccg
     6121 ccgcctggaa acccctgcag tcatagaaga aggcgaacgc cggcttcaag ccaaagccgc
     6181 gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgaa ccgcccttcc tgatttccga
     6241 cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc gcaccctgct
     6301 tgaagccgcc cccggcatcg tacgcggcac cagcaatgtc tttctcgcca aaaaactcgg
     6361 catcaccccc atcggcacca tggcgcacga gttcctgcag gcattccagg ccctcgacgt
     6421 acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat accggggcga
     6481 tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg atttcgacct
     6541 ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt acgtttgggg
     6601 cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca aaatgctgac
     6661 cttctccgac gggctggaca tcgaacgctc ttgggcattg caccaatatt tcaaagaccg
     6721 cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc atacgccctt
     6781 gaatatcgtc ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc tgtccgactc
     6841 tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc aagtgttcga
     6901 cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc tgtttctgcc
     6961 gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg aacttcatga
     7021 acctacatca aaccgtcgaa cacgaagccg ccgccgcctt tgccgccgca ggcatcgccg
     7081 acagccctat tgttttgcag ccgaccaaaa acgccgaaca cggcgatttc caaatcaacg
     7141 gcgtgatggg tgcggcgaaa aaagccaaac aaaacccgcg cgagttggcg caaaaggtcg
     7201 ccgaagcatt ggcggacaac gccgtgattg aaagcgcgga agtcgccggt ccgggcttca
     7261 tcaacctgcg cctgcgcccc gaatttctcg cgcaaaacat tcagacggcc ttgaacgacg
     7321 ctcgtttcgg cgtggcaaaa accgacaaac cgcaaaccgt cgttatcgac tattcttcgc
     7381 ccaatctggc gaaggaaatg cacgtcggcc acctgcgttc cagcatcatc ggcgacagca
     7441 tttcgcgcgt gttggcattt atgggcaata ccgttatccg tcaaaaccac gtcggcgact
     7501 ggggtacgca gttcggtatg ttggtcgctt atttggtcga gcagcaaaaa gacaatgccg
     7561 cgttcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc tttgacgaag
     7621 accctgcctt tgccgacacc gcacgcgaat acgttgtgaa gctgcaaggc ggcgatgaaa
     7681 ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc caagccgttt
     7741 acgacacgct gggcttgaag ctgcgtcctg aagacgtggc aggcgaatcg aaatacaacg
     7801 acgatttgca gcccgtggtc gatgatttgg ttcaaaaagg tctggcggtt gaggacgacg
     7861 gcgcgaaagt cgtgttcttg gacgaattta aaaacaaaga aggcgaaccc gccgcattta
     7921 tcgtgcaaaa acaaggcggc ggcttcctct acgcctccac cgatttggcg tgcctgcgct
     7981 accgcatagg ccgtctgaaa gccgaccgcc tgctgtacgt cgtcgaccac cgccaagccc
     8041 tgcacttcga acaacttttc accacttccc gcaaagcagg ctatctgccg gaaaacgtcg
     8101 gcgcggcatt tatcggcttc ggcaccatga tgggcaaaga cggcaagccg ttcaaaacgc
     8161 gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgag cgcgccaccg
     8221 ctttggtgaa agaaaaaaat cccgaattgg gtgcggacga agccgctaaa atcggtaaaa
     8281 ccgtcggcat cggcgcagtc aaatacgccg acttgagcaa aaaccgcacc agcgactatg
     8341 tgttcgactg ggatgccatg ctctcgtttg aaggcaacac cgccccctat ctgcaatacg
     8401 cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggatgca aatgcgccaa
     8461 ccgttttgac cgaaccgctg gaaaaacagc ttgccgccga gctgctgaaa tttgaagacg
     8521 tactgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac ctctatcaaa
     8581 ttgcgaccct gttcagccgc ttctacgaag cctgtccgat actcaaagcc gaaggcgcaa
     8641 gccgcaacag ccgcctgcaa ctggcaaaac tcaccggcga cacgctgaaa caaggcttgg
     8701 atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga ttgcggacaa
     8761 cagcctcgcc atccttatcc gaatctgaaa aaagcggcgc gatacaccgt atccgccgcc
     8821 cctcccaaaa tgcgaaacaa acaaacgcca agcaagcaag caagcaagca agcaagcaag
     8881 caagcaagca agcaagcaaa aaattataac ccccttcctg ccgacgcacg cactttccgc
     8941 gcggcgcatt ccccttttcc cgcccctcaa atccgccttt tcttcaggca gggtttcagc
     9001 ccgcctcttt tccctgtttt cctttccccg acacgcgtgc gctccccctg ccgcactgtg
     9061 ctgcactttc gcgcccggac ggcatcgttc cgccatccgg ttctctgttt tacatacccc
     9121 tgtttcagaa agaaatgcag atgtttcaac acacaggacg acacataaag caccgcccta
     9181 tgtgttgccc tgatttggaa ggggttacgc ctcccaaata aagtctgatc ctgccgcccc
     9241 gaaggacaga tgtccgagtg gcgaagtttc aaccgaaaag gaaatacgat gaatattcac
     9301 accctgctct ccaaacaatg gacgctgccg ccattcctgc cgaaacggct gctgctgtcc
     9361 ctgctgatac tgcttgcccc caatgcggtg ttttgggttt tggcactgct gaccgccacc
     9421 gcccgcccga ttgtcaattt ggactatctt cccgccgcgc tgctgatcgc cctgccttgg
     9481 cgtttcgtca aaattgccgg cgtattggcg ttttggctgg cggttttgtt tgacgggctg
     9541 atgatggtga tccaactctt cccttttatg gatctcatcg gcgccatcaa cctcgtcccc
     9601 ttcatcctga ccgcccccgc cccttatcag ataatgaccg ggctgttgct gctgtatatg
     9661 ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa ccgacttccg gcacattgcc
     9721 gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg gccatttgag ttactacgac
     9781 cggggtcgga tggccaatat cttcggcgca aacaacttct actacgccaa aagtcaggcg
     9841 atgctctaca ccgtcagcca gaatgccgac tttattaccg ccggcctggt cgatcccgtc
     9901 ttcctcccct tgggcaatca acagcgtgcc gccacgcatc tgaacgagcc gaaatctcaa
     9961 aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg ccaatcccga acttcaaaac
    10021 gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt
    10081 tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg aactgtgtgc ctacggcggt
    10141 ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat ttgcccgctg cctccccaac
    10201 cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg gcgcgggcag ttcgctttac
    10261 gaccgcttca gctggtatcc gagggcgggc tttcaagaaa tcaaaaccgc cgaaaacctg
    10321 atcggtaaaa aaacctgcgc cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa
    10381 gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt actggatgac gctgaccagc
    10441 cacgccgact atcccgaatc cgacattttc aaccacaggc tcaaatgcac cgaatatggc
    10501 ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa
    10561 ctggcggatt tgatccaacg ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac
    10621 catccgccgc ccgtcggcaa cctcaatgaa accttccgct acctcaaaca ggggcacgtc
    10681 gcctggctga acttcaaaat caaataacaa caatgccgtc tgaacgcacc aacagccttc
    10741 agacggcatt ttgcagacag accgaccctt caagcccact tttttcatca tctccgataa
    10801 attgctttgt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag
    10861 agaacgattc tctaaggt
//
LOCUS       DAWISC010000055        10854 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWISC010000055 DAWISC010000000
VERSION     DAWISC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.055"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(13..216)
                     /locus_tag="WIW87_001007"
     CDS             complement(13..216)
                     /locus_tag="WIW87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174182.1"
                     /translation="MDTTLKNKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(194..430)
                     /locus_tag="WIW87_001008"
     CDS             complement(194..430)
                     /locus_tag="WIW87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ5174183.1"
                     /translation="MMEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRN
                     GETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            967..4401
                     /gene="dnaE"
                     /locus_tag="WIW87_001009"
     CDS             967..4401
                     /gene="dnaE"
                     /locus_tag="WIW87_001009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ5174184.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLDNGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEAYVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4549..4698
                     /locus_tag="WIW87_001010"
     CDS             4549..4698
                     /locus_tag="WIW87_001010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174185.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(4835..5767)
                     /locus_tag="WIW87_001011"
     CDS             complement(4835..5767)
                     /locus_tag="WIW87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5174186.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(5837..7951)
                     /locus_tag="WIW87_001012"
     CDS             complement(5837..7951)
                     /locus_tag="WIW87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ5174187.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRRETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            8111..8770
                     /locus_tag="WIW87_001013"
     CDS             8111..8770
                     /locus_tag="WIW87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5174188.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(8858..9826)
                     /gene="ispH"
                     /locus_tag="WIW87_001014"
                     /gene_synonym="lytB"
     CDS             complement(8858..9826)
                     /gene="ispH"
                     /locus_tag="WIW87_001014"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ5174189.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(9855..10352)
                     /locus_tag="WIW87_001015"
     CDS             complement(9855..10352)
                     /locus_tag="WIW87_001015"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ5174190.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(10558..10662)
                     /locus_tag="WIW87_001016"
                     /pseudo
     CDS             complement(10558..10662)
                     /locus_tag="WIW87_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2473 a         3095 c         2847 g         2439 t
ORIGIN      
        1 agccgaaccg tttcagacgg catcgtccga ccaacccgct tctttcaatt tccgccgttg
       61 cgcgtcgtag cgggccttat ctgcccaata aatcgtctga atgcacgcct cgccgcagtc
      121 actgccgcag cattcccacg actcggggcg gacgggttcg tctaaaagcg gctcgcccaa
      181 aagggcttcg gctttattct tcagggtcgt atccatcggc gatttccaag cgcgcgccgt
      241 caaactcgat gacttcgcca ccgcgtattt tggccgtttt gcgggtttcg gtttcgccgt
      301 tgcgcaacac cagcccttcg gcgataaacg ccttcgcctg cccgccgctt tcggcaagtc
      361 cggccaattt caagaggtcg cacaaggcga tgtattcgtt gtcttcgaga tagacagtgg
      421 cttccatgat gttcccttgc agaaagaggc cgttattgta gcacctgccg ccgccgtacc
      481 caaaattacc gcaaaaccgg cagcacgtcc caccccgcct gttccgtaaa aatgccgtct
      541 gaaaccccat atgccgccat ccgatcaaag aaatcctgtc caacagcagg ctgcaaatcc
      601 tgttccccga cgaatccgca ttgacgctga tgcacatcct caaacgcaaa ctgcccgata
      661 cactggtcat cggcatcagc catcaaaacg aaatcaaaaa cctgtttgaa cgtaaaatta
      721 atttaactgc ttgccatccg tccgaataag gtatatagtt ctttataact agtttgatag
      781 ttctttatat ctatcaatac tccttgggaa gcctccacca cacggtagga ggcatttttt
      841 tgccgtcgta aaagctcaaa aacatttgca ggtcatgccg tctgaatccg aaacagcatt
      901 acctacaccg ccatctaaag acaaccctgc tacaatacgc cttttattgt tcacgccgat
      961 tttgccatga ccgagccgac ctacatcccc ctgcgcctgc ataccgaatt ctcgattacc
     1021 gacggtatgg tgcggattaa aaaactgatt gccaaagcgc aggaatacgg tttgcctgct
     1081 ttgggcatca gcgatttgat gaacgaattc ggcttggtga aattctataa agcctgccgc
     1141 agcgcgggga ttaaacccat cggcgcggcg gacgtgtgga taggcaatcc gaatgcgccc
     1201 gacaagccgt tccgcgctat gctgattatc cgcaacgatg cgggttatct gcgcttgagc
     1261 gagcttttga cggaggccta tgtcggcaaa gaccgcaata tccatcatgc ggaactcaac
     1321 cccgaatggc tggacaacgg cgacaacagc ggcttgattt gtttgagcgg cgcgcattac
     1381 ggcgaagtgg gcgtgaatct gttgaacggc aatgaagatg cggcgcgtgc ggcggcgttg
     1441 aaatatgcgg cgtggttccc cgatgcgttt tatttagagc tgcaacgcct gcccgaacgc
     1501 cctgaatggg aggcttacgt ttcgggcagc gtgaaactgg cggaggaatt gggtttgccg
     1561 gtggtggcga cgcatccgac gcagtttatg agccgcgacg acttcaacgc gcacgaggcg
     1621 cgggtgtgca tcgcgggcgg ctgggtgctg acggacaaga aacgtccgcg cgatttcacg
     1681 ccgagccagt ttttcattcc accggaaacc atggccgaac gtttcgccga tttgcctgaa
     1741 gccttggaaa acacggtaga aattgccaaa cgctgtaatc tgcacatcac attgggcaaa
     1801 aacttcctgc cccttttccc cacgcccgac ggtttatcac tcgatgacta tctcatcaaa
     1861 ctctccaacg agggtttgca ggaacgtatg gttcagcttt atcccgacga ggcggagcgg
     1921 gcggcaaaaa tgccggaata tcaggaacgt ttggattttg agctgaacat catcatccaa
     1981 atgaaattcc ccggctattt ccttatcgta caagacttta tcaactgggc gaaaacacac
     2041 ggctgtccgg tcgggccggg ccgtggttcg ggcgcgggtt cgctggtggc gtattcattg
     2101 aagattaccg accttgatcc gctcaaatac gcgctgctgt tcgagcgttt cctaaacccc
     2161 gaacgcgttt ctatgcccga cttcgacgtg gacttttgcc aaagcaaccg cggccgcgtg
     2221 attgaatatg tgcgcgagaa atacggcgcg gaggcggtca gccagattgt taccttcggc
     2281 acgatgtcgt ccaaagcggt catccgcgac gtcgggcgcg tgttagagct gccgtttatg
     2341 ctgtgcgaca aactgtccaa gctgattccg ttggaagcca acaaacccct gagtttggaa
     2401 aaagccatgg agaccgagcc acagattcag gaattaatcg aagcggaaga agcggacgaa
     2461 ctgattacgc tggcgaaaaa gctggaagat ttaacgcgcg gtttgggtat gcacgcaggc
     2521 ggcgtgttga ttgcgccggg caagatttcc gattacagcc ccgtgtatca ggcggacgaa
     2581 tccgcctcgc ccgtatccat gtacgacaag ggcgacgtgg aagatgtggg tttggtgaaa
     2641 ttcgactttt tgggtctgcg caacctgacc attatcgaaa tggcgcagaa caacatcaaa
     2701 aacactaccg gcgacatcat cgatgtcggc aaaatcccgc ttgacgacca ggtcgcctac
     2761 caaatcttcc gcgatgcgaa caccaccgcc gtcttccagt ttgagtcgac cggcatgaaa
     2821 aaaatgctga aaacggcgca cacgaccaag tttgaagaac tcatcgcctt cgtatcgctc
     2881 taccgccccg gcccgatgga caacattccc gacttcgtcg cacgtatgaa ggggcaagaa
     2941 ttccaataca tccatccgct actcgaaggc atcctcgcgc cgacctacgg gattatggtg
     3001 tatcaggaac aagtgatgca ggcggcgcaa attatcggcg gctactcgct cggcggcgcg
     3061 gacctgctgc gtcgcgccat gggtaagaaa aaacccgaag aaatggtgaa acaccgcgaa
     3121 atcttcgccg aaggcgcggc aaaacaaggc atttcgcgcg aaaaatccga cgaaatcttc
     3181 aactacatgg aaaaattcgc cggctacggt ttcaacaaat cccacgccgc cgcctacgcc
     3241 ctgatttcct accagaccgc atggcttaaa gcgcactacc ccgccgaatt tatggcggcg
     3301 accatgtcgt ccgaattgga caacaccgac cagctcaagc atttctacga cgactgccgc
     3361 gccaacggca ttgagttcct gccgcccgac atcaacgaat ccgactaccg cttcacgccg
     3421 tatccggaca tgaaaatccg ctacgcgctc ggcgcgatta aaggcacggg cgaagccgcc
     3481 gtcgaatcca tcaccgccgc gcggcaaagc ggcggcaagt ttaccggtct gttggacttc
     3541 tgcgagcgcg tcggcaaaga acacatgaac cgccgcaccc tcgaggccct gatacgcggc
     3601 ggcgcgttcg acagcatcga acccaaccgc gccatgctct tggcgaacat cgacctcgct
     3661 atggacaacg ccgaccaaaa agccgccaac gccaatcagg gcgggctttt cgacatgatg
     3721 gaagacgcca tcgaaccggt gcggctcatc gacgcgccga tgtggagcga atcggaaaaa
     3781 ctcgccgaag aaaaaaccgt catcggcttt tacctgtccg gccacccgtt cggcccgtat
     3841 gcccaagaag tccgccaaat cgcaccgacc aaattagacc gtctgaagcc gcaagacagc
     3901 gtgcgcctcg ccggattcgt taccgccgtg cgtacgatga tgggcaaacg cggcaaaatc
     3961 gccttcgtca gcctcgaaga tttgagcgga caggttgaaa tcatggtcgg cggtcagacg
     4021 ttggaaaact gcgccgactg cctcaaagcc gaccaagtgc tgattatcga atccaaagtc
     4081 agccgcgacg actacggcgg cggcgacggg ctgcgtattc tggcaaacca agtcatgacc
     4141 ctgcaaacgg cgcgcgaacg ctacgcccgc agcctcagcc tcgccctcgc cccgcatcac
     4201 gacatcggcg gactggtacg gctgctcgcc gcccaccaac tgcccgacac gccgcgcatc
     4261 ccgctgcaac tgtcgtatgc caacgaaaaa gcgtcgggca ggcttcaagt gccgccgaaa
     4321 tggacggtta caccgagctc cgcattgttc ggcgaactgg aaacattgct cggcagccgg
     4381 tcggtgcgcg tcaactggta acccaaaata taaatgccgt ctgaagccca aaaaccggtt
     4441 tcattcgtac tttattcgaa tgattgaata aaagtaactg ccaagaaaaa cgtatttttt
     4501 ggttatttcg ccagtctaaa tagagcaacc gggacgattg atatccgtgt gcatgacaca
     4561 gacagcacca aagggaaaaa cggcattttc caaagtatcg gtatcaaaac cgccctttca
     4621 ctccaaaaat accaaatcga caaaccgggc aaagaaatca gaccgtgccg cctgaaaaaa
     4681 cgcccacccg tccgttaaat taacctatcc ctgtttcaga cggcctgaag cagggatttt
     4741 tatatcaaaa taaaatgaga aagggagcaa taacccttag gtagctcttg ttattttccg
     4801 atgcaaaaca aagcagtcat atatttaatt ccccctacct ctgccaagcc ttcctcaaat
     4861 attcgacgca atcggtcagc gagtagaacg ggacattgcc gtggtcggca ttcggatatt
     4921 cccggaaaaa gacggcggcc ccgtgcctgt ctaactctgc cgccatttgt tcggcctgcc
     4981 ctgccatatc gcgttcttcc ctgcgtttac aatcgctacc ccgttctagc gcgccgatgt
     5041 tgaggcagac atcgatgccg tttagccggt tttcagacgg cataaagtcg agtatccgcc
     5101 tgttgtgcca ccaaatcgat ggggatacga gccaatgccg tctgaaacgg cggtgggaaa
     5161 gcagggaata cagtccgaac agtgcgccga acgagtgtcc gaatacggcg gtttcattgc
     5221 ggttgagggt gtagcggctt tctaaaaagg cggtcagctc gctgtcgata aaggcggcga
     5281 agcggtctgc ctgtccgaac tgctgccgtt cgtctgctgt ggcgttgtct ccaagcggcg
     5341 gcgtgtagtc ggcggcacgt tgtgccaaat cgcgcacact gcctgtcgtg tagccgatac
     5401 cgacaatcag gcagggggcg ttgcttcggg taacggggtt gttcatcagc gactgcatga
     5461 tgttgaaaag tgcggggaaa aaggcttcgc cgtcgaggac aaagaggacg ggatagcctt
     5521 cagacggtat ttcgccgagt gttgccgtct gaatccgata gattcgcccc gtgcaggtgg
     5581 atttgatttc ggtttcaaag gcttggggca gtatggcagg ttggaatgtg tcggtcggta
     5641 tgggtttcat gatgttcggc ttgtgggtca gactggcggc aagcggtaaa accgaaccgg
     5701 caaagcggtc tgcctgccgg ttcggtctat tttccttcgc aatgccatac tccagttgtg
     5761 agagcatagg gtatgccgcg cagcttgttg tagtttgatg atgctgcggc tgccgtttca
     5821 gacggcatat tggtctttaa aaactgtaac gcaggtttgc cgtcaggctg cgctccgaac
     5881 cgggaatgtt aaaggtgctc tcgctgccga cgcgggcgta gtaatggcgg ttgaagatgt
     5941 tgtcggcgtt gatttgcagc ttcagtttgg gcgtgaagcg gtatgccgcc atcgcatcga
     6001 acgtggcata accgcctgca tgtatccctg cagatgaagt aatgccgctc atcgcgttca
     6061 cgccgccgcc gatggtcagc ccggacgtaa cttggtaagt cgtccacagg tttgcgctgt
     6121 gtttgggcat cagcaggaag atgccttcgt cgcgcgaatt ggaggcggtt ttgatttggc
     6181 tgtgcaggta gctgtaacct gcatggattt gccatttcgg tgtcatcgcg ccgctgattt
     6241 cggtctcaac accttccatc acgcgtttgc ccaatgcggc gtaacgggtt tttttgttgt
     6301 ttgagtccag cggtgcggcg gcgtttttat ccttcatgcg gtagaacgaa acccgggtat
     6361 tgaggcggtc gtccatgtag ctgcctttgt agccgatttc aaactggttg ccttcgcgcg
     6421 gtttgagcag cttgccgtcg gtgccgatgc tggtttgcgg tgtgtagagt tgggaggcgg
     6481 aagcgtacag gctgttgctg ccgtctatat cgtaaaccgc gccggcgtag cttgtaaatt
     6541 tggttttcga agctttatgc agggttttgc cgtcgcccga ctcgattttg tgatgtccta
     6601 cacgtccgcc tgcaatcaac gacaaacctt ccagaggacg gaacaccgtc ttggcataca
     6661 aaccggtttc gtcgaggttt tcttcggtaa cggagtgatt gaaacctttg tttccggcgc
     6721 gggcgttctg aagtatgccg ttataaggca aagcgcggaa accatctaaa gcgacgcttt
     6781 ttgacaaagt cgaacgccct tgttcattag tactgcgcaa gcggttgtag tctgcaccaa
     6841 tcacaaattc gttggcggtg ttgcccaagg caaacggacg gctgtaactt gcgtcaaccg
     6901 caaaggcttt ttgtttaatg tccgtaccca aacccgctac gtcggcttgt ccggtattgt
     6961 tgagtttgct gcccgcaaac gtataattgg aatcggcttt ccgatcggaa tagcgcatac
     7021 cgactttgcc gtagccgccg ttgccgaagt aatgtttcaa atcggcgaac acgtcgtggc
     7081 tgtgcatttt aaatttgttc caatccgcgc cgacaaatac gtgttgcggc agggacggta
     7141 atttgttatt ggcatcggca ggcaggccgt tgtacggcgc gaggcggcgt tgctggtaaa
     7201 gatagcccgc gcccaaaacc gtatcggggt tgatgtccca atccgccgcc gcgtagaagg
     7261 tttcgcgccg gttgtttttc tcggcgggac gcggagacgc gccgacggtc tgcgccatca
     7321 cgcggccgcg cacgctgccg tctgaattga ggctgcccga tacgtccgcc tcggctttat
     7381 attgtttgtg cgtaccgaac cctgccgccg catgaccttg gaacgctttg gtcgggcgtt
     7441 tgcgcaccag attcacgatg ccgcccatct cgccgctgct gtcgaacagt ccgctcggcc
     7501 cgcgcatcac ttccacgcgg tcgaaggcga acaggttggg cagcgtgccg ttgatactct
     7561 gcatctgcgc gggcaggccg tcgatgttgt attcgctgta ttcgtaaccg cgcgcgtaaa
     7621 ccgaagagcg tccgtcgtcg ttgctcaaca cgcgcaggcc gggcgttttg cgtgccaact
     7681 ggtcaaacgt atcaacattg cggtctttga cctgctggtt ggtaatgatg ctgacggatt
     7741 gcggaatttc gcgcaaagaa gcggggattt ttgtaccgac ggtggcggca aacgagctgt
     7801 aatcgccgtt tttctcggtg gcaatcgcgt tgtaagaacg ctgaccctta atatggacgg
     7861 tttccaaacc ttccgtttgt gcggcaaaaa ccgaagacga gagtgctgcc aaaaccgtgg
     7921 cggcggtcat attgatgcgg aaaactgaca taaactgtcc cattcacata aatgataatg
     7981 gttctatttt aataaagcgc aacgcggctt gttcggaaaa acatatcgcg cagccgacaa
     8041 attttgtcga aaatgcgaca cgtctgcgtt ttccgcataa aatttgcttt tttactgcaa
     8101 ccaacctgct atgaccacgc ccaaactcat catcttcgac tgggacggca cgcttgccga
     8161 tacgacccaa cccatcatcg acaccatgcg ccgcagcttc gccgaatgcg gttttccgcc
     8221 gcccgaagcg gaacgcgtcc gcagcctgat tggctacagc ctgcccgaaa tcatccgcac
     8281 cctgctcgaa atgccgtctg aaaccgccgt tgccgacatc acacgcactt attccgcaca
     8341 ttacctcaat cccaacaacc gcaatatgtc cttatttccc gatgccctgc cctgtctgga
     8401 caagctcaaa gcacaaggat actggcttgc cgtcgccacg ggcaaagggc gggcgggttt
     8461 ggacaacgcc atcagtcaaa ccgccaccgg cggctattgg ctcgccaccg cctgcgcggg
     8521 ggaatatccc tccaaaccct cgcccgaaat ggtattcgga atctgcggcg aactgggact
     8581 cgacccgaaa gaggcattgg tcgtcggcga tacggcgcac gacctgcata tggcggcaaa
     8641 cgcaggcgcg gcggcagtcg gcgtggccac cggcgcacat tcgcgcgaac agctccttag
     8701 cgcaccgcat ctcgccgtat tggacggttt gtccgaactg cccggttttc ttgcacaaca
     8761 ttacgcctga ttggtttccg catccggcac acggcaaaaa tgccgtctga agcctgttca
     8821 gacggcattt gtgttgccca aacattcaac gcctgcgtca acgtttgcac aaatcgggtt
     8881 tggtttcgcc ctcgcggcgc aactctttgg gcaggacgaa cacaatgctt tcttccgcac
     8941 cctcgccttc gcgtacggtt tcgtgccccc atccgcgtat ggttgccagt acttcccgca
     9001 ccaacacttc gggcgcggac gcgcctgccg ttacgccgac tttgtttttg ccctcaaacc
     9061 atgcgcgttg caggtagcct gcattatcca ccatatacgc atcgattccg cgcgatgccg
     9121 ccacttcgcg caagcggttg ctgttggacg aattgggcga accgaccaca atcacgatgt
     9181 cgcactgttc tgccaactct ttgacggcgg tttgccggtt ggtcgtcgca tagcagatat
     9241 cttccttgtg cggattgcgg atattgggga aacgcgcgtt cagcgcggcg atgatgtctt
     9301 tggtttcatc gaccgagagc gtggtttggc tgacataggc gagtttgtcg gggtttctga
     9361 cttcgagttt tgccacatct ccgaccgttt cgaccaaaag cattttgccc ggcgcaagct
     9421 gccccatcgt tccttcgacc tcgacgtgcc ccttatgccc gatcatgatg atttcacagt
     9481 cttgggcatc cagtcgggcg acttccttat gcactttcgt caccagcggg caagtcgcat
     9541 caaacacgcg gaaaccgcgc tccgccgctt cttgccgcac cgccttcgat acgccgtgtg
     9601 ccgaataaac cagtgtcgcg cccggcggca cttccgccaa gtcttcaata aacaccgcac
     9661 ctttttcacg caggttgtcc acgacgaatt tgttgtgaac gacttcgtgg cgcacataaa
     9721 tcggcgcgcc gaactcttcc aaagcacgtt cgacaatact gattgcccga tccacaccag
     9781 cgcagaagcc gcgcggattg gcaaggatga tggttttctc gttcataagc ccggtatttc
     9841 gttttcagac ggcatcaata tttttcttct tgggttttac ggtggacgat gttgtccaac
     9901 accgccaaca ccgcaccgac gcagataaag ctgtcggcaa tattaaaggc gggataaaac
     9961 caattttgcc aataaaacaa taagaaatcg acgacatgac cgtgtatcag gcggtcgatg
    10021 acattgccta acgcaccgcc gataatcatt gccgcacccg ttttgccgag ggttgcaaac
    10081 tcatcgcgca agatggcgcg taccaaatac gcgctcaccg ccaccgccag caccaaaaaa
    10141 aagtattttt gccagccgcc ctgatcggca aggaagctga acgccgcacc cgggttgtac
    10201 accagcgtca gatcgaaaaa ggaaggaatg acattgacgc gttcccgata ctgaaacgac
    10261 gacagcaccg cccacttcga ccactggtcc agcacgatgg cggcaagtgc caatacccaa
    10321 tagcgcgttt tacttgaaac agatgaagac atatttttca acagccggta aaagagtacc
    10381 attttacccg aaaaccccct ttcctgtacc cgaaacggca aatgccgtaa tcttaaaacc
    10441 cgtcattccc gacaacaccg taatctcgaa acccgtcatt cccgcgtagg cgggaatcca
    10501 gacctgtccg cacagaaact tatcggataa aaacagttgc ccaaaccccg cgttctatag
    10561 tggattaaat tcaaaccagt acggcattgc ctcgccttgc cgtactattt gtactgtctg
    10621 cggcttcgtt gccttgtcct gatttaaatt taatccacta tagattccca cttccgtggg
    10681 aatgacggtt cagttgcatt ccgacaacac cgtaatcttg aaatccgtca ttcccgcgca
    10741 ggcgggaatc tatcggaaat gactgaaacc tcgagattct agattcccac tttcgtggga
    10801 atgacggttc agttgcgttc caacaacacc gcaatctcga aatccgtcat tccc
//
LOCUS       DAWISC010000056        10483 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWISC010000056 DAWISC010000000
VERSION     DAWISC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.056"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(278..1075)
                     /locus_tag="WIW87_001017"
     CDS             complement(278..1075)
                     /locus_tag="WIW87_001017"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ5174191.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1230..4079
                     /gene="uvrA"
                     /locus_tag="WIW87_001018"
     CDS             1230..4079
                     /gene="uvrA"
                     /locus_tag="WIW87_001018"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ5174192.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPFD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            4113..5708
                     /locus_tag="WIW87_001019"
     CDS             4113..5708
                     /locus_tag="WIW87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ5174193.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6015..6239)
                     /locus_tag="WIW87_001020"
     CDS             complement(6015..6239)
                     /locus_tag="WIW87_001020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174194.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(6268..7158)
                     /gene="sucD"
                     /locus_tag="WIW87_001021"
     CDS             complement(6268..7158)
                     /gene="sucD"
                     /locus_tag="WIW87_001021"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ5174195.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7169..8335)
                     /gene="sucC"
                     /locus_tag="WIW87_001022"
     CDS             complement(7169..8335)
                     /gene="sucC"
                     /locus_tag="WIW87_001022"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ5174196.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8434..8721)
                     /locus_tag="WIW87_001023"
     CDS             complement(8434..8721)
                     /locus_tag="WIW87_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174197.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8846..10279)
                     /gene="lpdA"
                     /locus_tag="WIW87_001024"
     CDS             complement(8846..10279)
                     /gene="lpdA"
                     /locus_tag="WIW87_001024"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5174198.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2719 a         2661 c         2440 g         2663 t
ORIGIN      
        1 ctgtaacgtt aagttcccgg attcccactt tcgtgggaat gacgcggaaa ggtttctgtt
       61 ttttaagtgt taggtaacta ataagtcgtc attcccgcgc aggcgggaat ccagtccgtc
      121 cggtttcagt cgtttccgat aaatcgtcgt aacgttaagt ttctagattc ccactttcgt
      181 gggaatgacg gtgtgaacat tagcaaaaat tcaaaaaacc aaacttcctc attcgtgtga
      241 atgatggtac tgatattttc tatttcagtc cgcaaaatta atctttcaac acatcttgca
      301 cagccgcctg aatcttagcc gccgctgcct cgatttgccg ttgttcggca gatgggtttg
      361 ccgctgtttc aaccggagca gtttgtaaag caggctcaga ttcaggctca gattcagttt
      421 gcggcgcagt cagcggcaaa cgcgccaata cagtgctgac gccggttact ttatcgccaa
      481 tcgccacttg cgcctgcgca tcgacaggca gatacatatc cacgcgcgaa ccgaagcgga
      541 taaagccata acgttcgccg cgggacagtt tcgcacctgc ttgggtgtag cacaaaatac
      601 ggcgcgccac caaaccggcc acttgaacaa aagtaatttc acgacctgaa gccgtagtcg
      661 ccaacaccgc attacgttcg ttttccgtgc tggctttgtc caaatccgca ttcacgaatt
      721 tgcctttgtt atagaccact ttcgttaccg tacaatcggc aggcgatttt tgcgaatgca
      781 cgttgaacac gttcataaaa atactgattt tcaaagcatc gacatcacga tacggatcgc
      841 gtgcgcgttc gaccaccacg atacggccgt caaccgggct caacaccgct tcaggattta
      901 gcggaatctc acgcgcaggg tcgcggaaaa actgcaatgc aaatacggta aacacccaaa
      961 acggcaaaga ccaccagccg caacatatcg acaccagcag gctcaaagcc aaaccgccgc
     1021 caataatcgg ccagccctca cgggcgataa tcgggtgggg gtaaagacgg ttcatttgtc
     1081 atccttttgt tcaagcgggt taaaattgcc gacattatag cggaatctgc cgtactccgc
     1141 tggcaaatgc cgtctgaacg cggcttcaga cggcatccga gaaagtgcta caataccggt
     1201 tttcctttat ttttcaggcg gcatccatta tgtgcaacca tcatccccaa cattcgcacg
     1261 acaacgacac catccgcatc cgcggcgcgc gcacgcataa tttgaaaaac atcgatttag
     1321 acattccgcg ccacaaactc gtggtggtaa caggattgtc gggcagcggc aagtcgtcgc
     1381 tggcgtttga cacgctgtat gccgaagggc aacggcgtta tgtcgagagc ctttccgcct
     1441 atgcgcggca gtttttgcag atgatggaca aacccgatgt cgatttaatt gaaggtctgt
     1501 cgcctgcaat ttccatcgag caaaaatcca ccagccacaa cccacgctcc accgtcggta
     1561 cggtaactga aatccacgac tatctgcgtc ttttatacgc ccgtgtcggt accccgtatt
     1621 gtcccgaaca caagctgccg ctgtcgagcc aaaccgtatc gcagatggtc gatgccgtat
     1681 tgaaactgcc ggaagacacg cgtgtgatga ttcttgcccc caccgtgcgc gagcgtaagg
     1741 gcgagtttgt cgatttcttt gccgacttgc aggcgcaggg ttttgcgcgg gtgcgcgtgg
     1801 acggcgaggt ctatcagttg gacgaagtgc cgaagctgga aaaaaacatc aagcacaata
     1861 tcgacgtggt catcgaccgc gtgaaagtga aggcggacat caagcagcgg ctggcggaaa
     1921 gttttgaaac cgcgctgcgc cacggcaacg agcgtgcgct ggcgatggag atggacagcg
     1981 gcgaagaaca ttggttttcc gcacgtttcg cctgccccgt gtgttcgtac agcctgcccg
     2041 aattagagcc tcgccttttt tcgttcaaca acccgatggg ttcctgcccg acttgcgacg
     2101 gcttgggcaa caccaatttc ttcgaccccg aaaaagtggt cgcccatccc gaattgtcgc
     2161 tggcaacagg tgcgattgac ggttgggaca agcgcaacca attctatttc caaatgattc
     2221 aatcattggc gcggcattac ggtttcgatg tgcaggctgc ttgggaaaca ctacctgaaa
     2281 aagtgaaaaa agttgtgctg cacggctcag gcaaagaagt cattgatttc acttacctgt
     2341 ccgaacgcgg caccaccttc aaccgcagcc acgcctttga aggcatcatc cccaatctcg
     2401 aacgccgcta ccgcgaaacc gacagcgaaa ccgtgcgcga aaaactgcgc gaataccaaa
     2461 accaccgcgc ctgcccaagc tgcggcggcg cacgtttgcg caaagaagcc cgctacgttt
     2521 acgtcagcgg tgagccgttg cacgaagttt ccgcctggcc gctcaccaaa acccaccaat
     2581 tctttgaaac actggattta gacggcaaca aaaaacaaat cgccgaaaaa atcctcaaag
     2641 aaatcaccga acgcctcggc ttcctgatta acgtcgggct ggattacctg aatttaagcc
     2701 gctccgccga aaccctctcc ggcggcgaag cccagcgcat ccgcctcgcc agccaaatcg
     2761 gcagcggcct gaccggcgtg atgtatgtac tggacgaacc ctccatcggc ctgcaccagc
     2821 gtgacaatga ccgcctgctg gccaccctca aacgcctgcg cgatttgggc aacagcgtga
     2881 ttgtggtcga acacgacgaa gacgccatcc gcgaagccga tttcgtggtc gatatgggcc
     2941 ccggcgcagg cgaacacgga ggcaacgtac tgattgccga cacgcccgaa aacgtcgccc
     3001 aatgcgaaaa ctccgttacc ggacaatacc tcagcggcaa aaaatccatt gccgtgccgt
     3061 ctgaacgcac gcccgtcaat cccgaccgga tgctcgtcct caaaggcgca cgcggcaaca
     3121 atctcaaaaa cgttaccctt gagctgccgc tcggtttgat tacctgcatc accggcgtgt
     3181 ccggcagcgg caaatccacc ctgattaacg acaccctcgc caaaattacc gcccgcgaac
     3241 tcaaccgcgc ccaagaagaa cccgccccat tcgacgacat ccacggcctc gaacacctcg
     3301 acaaagtcat caacgtcgat cagtccccca tcggccgtac tcctcgctcc aaccccgcca
     3361 cctacaccgg cctgttcacc cctatccgcg aactcttcgc cggcgtcccc ctctctcgcg
     3421 aacgcggcta caacgtcggc agattctcct tcaatgtcaa aggcggccgc tgcgaagcct
     3481 gccaaggcga cggcgtgatt aaagtcgaaa tgcacttcct gcccgacgtg tacgtcccct
     3541 gcgaagtctg ccacggcaaa cgctacaacc gcgaaacgct cgaaatccaa tacaaaggca
     3601 aaaacatcag ccaagtcctc gacatgaccg tcgaagaagc ccgcgaattt ttcgacgccg
     3661 tccccaccgt atcgcgcaaa ctgcaaaccc tgatggacgt aggcctaggc tatatccgcc
     3721 tcggacagtc cgccaccacc ctctccggcg gcgaagccca acgcgtgaaa ctcgccttgg
     3781 aactctccaa acgcgacacc ggcagaacgc tctacatcct cgacgaaccc accaccggcc
     3841 tgcacttcgc cgacatcgcc ctgctgctgg aagtcatagg ccgtctgaaa ggcaaaggca
     3901 actcgatagt gattatcgag cataatctgg atgtgattaa aacagcggat tggattgtgg
     3961 acttggggcc ggaaggcgga gatggcgggg ggaggattat tgctaaaggt agtcctgaac
     4021 aggtggcgaa agttaagggg agttatactg ggaagtattt gaaggtggtt ttgaggtaag
     4081 tttaattcaa aattaaatag gagattatta agatgaaacc gttaaaaaca ttagaatttg
     4141 gatttgtgga tgctgcaaac tacagaagaa gagaaaataa agatttattt aaccgaatat
     4201 ttgtaaaagg agaatatttg gatgaattat gtgaaccaaa tatttcgttt ttaatcggag
     4261 aaaagggaac tggaaagaca gcatatgctg tttatttaac taataacttc tataaaaaca
     4321 tacatgccac tactaagttt gttcgtgaaa ccgattattc aaaatttatt cagctaaaga
     4381 aagcaagaca cttaactgtt tcagatttta caagtatttg gaaagtcatt ttatatctgt
     4441 tgatatcaaa tcaaatcaaa tgtaaagaaa acggaatatt atcttcaata tttaataaat
     4501 ttaaagcctt agatgaggct ataaatgaat attattatgg cgcttttgat ccggaaattg
     4561 tacaagcaat aactttaata gaaaattcaa aagaagctgc ggaaatgatt tttggaaaat
     4621 ttgttaaact aggtgaagag gaatcccaac aaataacttt tacagaaagt aaattccaag
     4681 caaatttagg ttttattgaa agaaaattta aagatgcttt atctcagtta aagctaaaag
     4741 ataatcatat tttgtttatt gatgggatag atattagacc atcacagatt ccatttgatg
     4801 aatatcatga gtgtgtaaaa ggtcttgcta acgccatatg gatgttaaat aatgatatct
     4861 tcccttccat taaagatagt aagggaagga tgagagttgt gttattgatt agacctgata
     4921 tctttgattc attaggttta caaaatcaaa ataccaaact tcaagataat tcagtatttt
     4981 tagactggag gacggattat aaatcttata gaagttcaaa gatttttggc gtttttgatc
     5041 atcttttgag aacccagcaa gaaaaacaag atagtttaga aaaaggcaac tcatgggatt
     5101 attattttcc atggaatgct cctaatttac atgatgagta taaaaattta acttcattta
     5161 ttagcttcct aagaaaatcg tattatcgac ctcgcgatat tcttcagatg cttactttgc
     5221 tacaaaaaaa taagaaaagt aaggaagatt atgtcgtagc agaagatttt gataatactt
     5281 cttttcaaag agaatactcg atatatttac ttggtgaaat caaagatcat cttttgtttt
     5341 attatagtca aagtgattat caaaatttcc tgaaattttt tgaattttta aacgggaaag
     5401 atagatttaa atatagtgat tttttaaaag catttgaacg tttgaaaaag cacttacaaa
     5461 caacatcagt ggaaatacct aaatttatga gtactgctaa tgagtttttg caatttttat
     5521 ttgacttgaa tgttattgct tatttagata acccagaaga tgaaacgaaa ccatatatcc
     5581 attggtgctt taaagataga aattatgcaa atatttctcc taaaataaaa actgaaactg
     5641 aatatttaat attttcagga ttatcaaaag cccttgatgt tggtactcca tttaagaaca
     5701 aacagtaact tgtatcccaa ccaaccgtgt gcgctacgca cacggttggt agctgacgtg
     5761 ttgcagtttc aacagagaaa gccgtagcct gcatacccaa ccgaccgcgt gcgtggctgt
     5821 gccacacgcc ctacctgtga attacacgga acttcaaagc caatacgaga gctttgcaaa
     5881 attcctttcc ctcccgacag ccgaaaccca aacacaggtt ttcggctgtt ttcgtttcaa
     5941 atatccacta attttacccc aataccccct taatcctccc aggatacccg ataatcaggc
     6001 atccgggccg ccttttaggc gcaaacaggc acacttagcc cgagaccttt gcaaaattcc
     6061 ccaaaatccc ctaaatgtct tggtgggaat ttagggaatt taggggattt tggggaattt
     6121 tgcaaaggtc tctgactgcc ccaaaagggc aaaaataatg tgggtatgtt gaggctgcct
     6181 ctttttgaaa tgacaaacaa aatgccgtct gaaacccgat ttccggtttc agacggcatt
     6241 tgatgttcaa tcagttgtca aacctgatta tgccaaacct tttgctttca acacttccag
     6301 catggtagtg cccaactcgg cagggctgcg ggtgtaagcg ataccggctt tttcgaaagc
     6361 ggcgaatttt tcttccgcag tacctttgcc gccggagata atcgcaccgg cgtgacccat
     6421 gcgtttgcct ttaggtgcgg taacaccggc gatatagccg acaacaggtt tgcttacgtt
     6481 ggattggatg tattcggctg cttcttcttc cgcagtaccg ccaatttcac cgatcatgat
     6541 gatggcgtcg gtatccgggt cttcttggaa aagtttcagt gcgtcgattt ggttcatacc
     6601 cggaatcggg tcgccgccga taccgataca ggttgattga cccaagccca gtttggtggt
     6661 ttgtgccacg gcttcgtaag tcaatgtacc ggaacgggaa atgatgccga tgcggccggg
     6721 agtatggatg tggcccggca taatgccgat tttgcactcg cccggagtaa tcacgcccgg
     6781 gcagttaggg ccgaccaaac gtgttccgtt accgttggtt tccaagtagc gtttggcttt
     6841 gagcatgtcc aaagtcggca cgccttcggt aatcacaacg accaagccta cgcctgaatc
     6901 aactgcttca acgatagaat ccaacacaaa cggagcagga acgtaaatca cggatgcatc
     6961 cgcgccggtt tctttaacgg cttctttcat ggtgttgaac acgggcaggt tcaggtgggt
     7021 ttgaccgcct ttgcccgggg taacgccgcc gacaacttta gtgccgtaag ccagagcttg
     7081 ttcggagtgg aaagtaccgt ttttaccggt gaaaccttga accaatactt tagtgtcttt
     7141 attaatcaat acgctcattc ttttctcctt aggcgtttac ggctgcaaca attttttcgg
     7201 ctgcgtcatt caggccgtct gcagaagtca gtttcagacc tgattcgttc aggattttcg
     7261 cgccgagttc ggcgttgttg ccttccaaac gaacaacgac aggaacgttg acgttgattt
     7321 ctttaacggc tgccacgatg gcttccgcaa tcatgtcgca acgtacgata ccgccgaaga
     7381 tgttgatcaa tacgccttga acggatttgt cttccagaat cagtttgaac gcttcaacca
     7441 cgcggtcttt ggttgcgccg ccgccaacgt ccaagaagtt ggcaggttgg ccgcctttca
     7501 gtttgatgat gtccatagtg gccatcgcca aaccggcacc gttcaccata cagccgatgt
     7561 tgccttccag ggcaacatag ttcaggtcaa attcagaagc tttcaactcg cgttcgtttt
     7621 cttgagattt gtcgcgcaat tcggcgattt tcggcaggcg gtagagcgcg ttgctgtcga
     7681 tgccgatttt gccgtccacg caggcgagcg cgccgttttc gcgaactgcc agcgggttga
     7741 cttcaaacag ggcgaagtca ttttcgacaa acgctttgta cgcaccggtc atcagtttga
     7801 cgaactcgtt gatttgtttg tctttcaagc ccagttggaa cgcaacttcg cgagcttggc
     7861 aaggttgcag gccgaccagc ggatcaacgg ttactttgaa gattttttca ggagtttcgg
     7921 cagcaacttt ttcgatttcc acgccgcctt cggtagaggc catgaatgta atgcggcggg
     7981 tagaacggtc aaccactgcg cccaagtaca gctcggtttg aaccggatac atgtcttcgc
     8041 aaaccaaaac actgttgaca ggttggccgt tggcatcggt ttggtaagtt accaagttgg
     8101 tgccaatcag gctttcagcc acttctttag cttcttcgcg gcttttaacg acttttacgc
     8161 cgcccgcttt accgcggccg ccggcgtgta cttgtgcttt gacaacagcg aatttgccgc
     8221 ccaatttgtc gtaagctgca gcggcttctt cgccgttgtg tgccaaaata ccgccttgta
     8281 cgggcaaacc gtagctagcc agcagttctt tagcctgata ctcgtgtaaa ttcatggatt
     8341 tctcctttag attagtggaa tgccccgagt ctgcctccaa atggcaagcc cgtgcgtttc
     8401 agactacctt tcggcggtcg gaaaatcgtc gtttcacttg ctgaaaggca accacatccg
     8461 cgccagcaga tacatggctg caccgcctcc cccgagaata aaaaagacga tgcctttttt
     8521 acgcgaattc ggacagagca gataaacggc aaggctgaaa ctgataaaca acaatgccac
     8581 gcgccaaacc aattctttat ctttgaaacg gttgagcgta taagtttcgg tcatcatcac
     8641 atacatctgc cacaatgcgg ccatacacat gctgatgatg cccgccggta taaagaaaat
     8701 accgactatt tctttgttca taatgaatgt aaatgctgtt tgtttgtctg acggaattat
     8761 aaatcagaaa acggagctct cggaacattc aatttgtcga aggccttaag gccgtctgaa
     8821 aaatttcaga cggccttaat gtctatcaac cgtgcaaagc gcgtttgtcg gccgccaatg
     8881 cagcttcgtg aaccacttcg gacaaggttg ggtgggcatg gataatgcgg gcgatgtctt
     8941 cgctgctggc gaagaattcg agcgcagtca cgccttcggt aaccaattcg ctgacaaccg
     9001 gaccaatcat gtgtacgccc aagatgcggt cggttttggc atctgccaac actttaaccg
     9061 tacctttggc tttgcccatt gccaatgcgc gaccattcgc accaaaacct gaagtacctt
     9121 ttttgtactc cacgccttcg gctttgagct gctcttcggt tttacccacc caagcgattt
     9181 caggatcggt gtaaatcacg aacggtacgt tgttgaagtc gatatgcggt ttttgaccgg
     9241 caatgcgttc ggcaacggca acgccttcgt cgctggcttt gtgtgccaac atcgggccgc
     9301 gaaccacgtc gccgattgcc catacgttag gcaggttggt acggcattcg ccatctactt
     9361 tgataaagcc gcgctcgtct ttttccaagc ctacggcttc cgcgttcagg cctttggtgt
     9421 ttggaatacg gccgatggca acgatcagtt tgtcgaatac ttcggttttg gcttcgccag
     9481 cagcagtttc gtaagcaacg gaaacacctt tgccttcaga cttgatgtcg ccgattttca
     9541 cgcccaattc gatgctcaga ccttgctctt tggtgaagta tttgaaggct tctttggcga
     9601 tttgttggtc ggcggcagcc aggaaagtcg gcgcggcttc aagaatggta acttccgcac
     9661 ccacgcggtt ccatacggaa cccatttcca aaccaatcac gccggaaccg atcacgccga
     9721 gtttggcagg tacttcggtc aggttcaatg caccttcgtt gtccaatacg ttcacattgt
     9781 cgatagcgac ttgtggcagc ggacgcggta cggaaccggt ggctacgatg acgtgtttgg
     9841 cttcgataac ggttttctcg cctttgttat cgacttcgat ttggtaagca tcgccatttt
     9901 taccggcaaa ggaagccgta ccgaacaggc tggttacttt atttttttgg aacaggaatt
     9961 tgacgccgcc ggtcagcttg gtcacgatgg catctttgcg ctcaatcatt ttggccgcgt
    10021 cgaattttac gtcgccgaca gtgataccgt gttcggcaaa ctcgtgttgc gcagcgtgga
    10081 aatgttcgct ggattgcaac agggctttag aagggataca gcctacgttc aagcaagtac
    10141 cgcccaatgc aggggcattg cctgctttgt taacgcctgc atcgacgcaa gcggttttga
    10201 aacccagttg cgcggcacgg atggcggcaa cgtatccacc cgggcctgca ccaatcacta
    10261 ctacatcata ttgagacatc gtgtcatcct ttgcttttaa cagtatctca aaataaaaac
    10321 gatacttttt gtttcttttt tattttgaat gactgcgttt tgatggtttt aagaaaaggc
    10381 cgtctgaaag tccatatctg ccgattcatt cgaatcaaat gtttcagacg gcctcaatcg
    10441 ttcgttgagc ctattgtagc acgatactac attacgctac atc
//
LOCUS       DAWISC010000057        10480 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWISC010000057 DAWISC010000000
VERSION     DAWISC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.057"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(182..394)
                     /locus_tag="WIW87_001025"
     CDS             complement(182..394)
                     /locus_tag="WIW87_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174199.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(406..687)
                     /locus_tag="WIW87_001026"
     CDS             complement(406..687)
                     /locus_tag="WIW87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174200.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGRHYRHSENGITLYDFIFFDGKPADEARFAAILTETCRRAVKKICETK"
     gene            complement(725..988)
                     /locus_tag="WIW87_001027"
     CDS             complement(725..988)
                     /locus_tag="WIW87_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174201.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            complement(1008..1580)
                     /locus_tag="WIW87_001028"
     CDS             complement(1008..1580)
                     /locus_tag="WIW87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEZ5174202.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1559..1870
                     /locus_tag="WIW87_001029"
     CDS             1559..1870
                     /locus_tag="WIW87_001029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174203.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1892..2767)
                     /locus_tag="WIW87_001030"
     CDS             complement(1892..2767)
                     /locus_tag="WIW87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ5174204.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2812..3102)
                     /locus_tag="WIW87_001031"
     CDS             complement(2812..3102)
                     /locus_tag="WIW87_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5174205.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3114..3359)
                     /locus_tag="WIW87_001032"
     CDS             complement(3114..3359)
                     /locus_tag="WIW87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5174206.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3352..3576)
                     /locus_tag="WIW87_001033"
     CDS             complement(3352..3576)
                     /locus_tag="WIW87_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5174207.1"
                     /translation="MEKPLNFKPISYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3848..4219
                     /locus_tag="WIW87_001034"
     CDS             3848..4219
                     /locus_tag="WIW87_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174208.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4221..4844
                     /locus_tag="WIW87_001035"
     CDS             4221..4844
                     /locus_tag="WIW87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174209.1"
                     /translation="MDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4837..5031
                     /locus_tag="WIW87_001036"
     CDS             4837..5031
                     /locus_tag="WIW87_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174210.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5055..5618
                     /locus_tag="WIW87_001037"
     CDS             5055..5618
                     /locus_tag="WIW87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174211.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5649..6596
                     /locus_tag="WIW87_001038"
     CDS             5649..6596
                     /locus_tag="WIW87_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174212.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6681..7184
                     /locus_tag="WIW87_001039"
     CDS             6681..7184
                     /locus_tag="WIW87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ5174213.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7198..7371
                     /locus_tag="WIW87_001040"
     CDS             7198..7371
                     /locus_tag="WIW87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174214.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            7296..7628
                     /locus_tag="WIW87_001041"
                     /pseudo
     CDS             7296..7628
                     /locus_tag="WIW87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7625..8035
                     /locus_tag="WIW87_001042"
     CDS             7625..8035
                     /locus_tag="WIW87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ5174215.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            8068..8601
                     /locus_tag="WIW87_001043"
     CDS             8068..8601
                     /locus_tag="WIW87_001043"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ5174216.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
     gene            8625..8735
                     /locus_tag="WIW87_001044"
     CDS             8625..8735
                     /locus_tag="WIW87_001044"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ5174217.1"
                     /translation="MSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTES"
     gene            8742..8843
                     /locus_tag="WIW87_001045"
     CDS             8742..8843
                     /locus_tag="WIW87_001045"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ5174218.1"
                     /translation="MHEYIRYYNNDRIKLKLKGLSPVQYRTQSLKAA"
     gene            complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="WIW87_001046"
     tmRNA           complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="WIW87_001046"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9586..10191)
                     /locus_tag="WIW87_001047"
     CDS             complement(9586..10191)
                     /locus_tag="WIW87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ5174219.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2554 a         2437 c         2661 g         2828 t
ORIGIN      
        1 ggtatttggg gagaaaacag ctgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
       61 ggaattttgc aaaggtctca gagtgagttt attttggggc ggcggcaggt cgggggcaag
      121 cggcgtgggg cttggttgtg gtttttaggt ttttgggggt aaaaaatgcc gtctgaactt
      181 ttcagacggc gtttgttttt tctatccaat cgaggaactg ccgccatttt tccagcggca
      241 tatcggcccg acgggtttcg gtatcgggtt cggcttccca gcggcctacc tgtatgtagt
      301 gcttcacccc gacgatttgc gccaactcgg cctgtgtcag tttgcagcgg ttgcgaaggg
      361 tgcggaggtt gtaaggcgtg tagccgagtt ccatgtcgtt gcggtttatt ttgtttcgca
      421 tatttttttg actgcccggc ggcaggtttc ggtaaggatg gcggcaaatc gggcttcgtc
      481 tgccggtttg ccgtcgaaaa aaataaaatc gtaaagggtg atgccgtttt cggaatggcg
      541 gtaatgcctg cccgggcatt cgccgtctgt ctgcctgtgg attttggcaa tcaggcgggg
      601 atagcggcaa tgggtaatga aacggcttgc gccgtcttgc ccgataatcc attcggggta
      661 gcggttgaat agtgcggctc tgttcatttt gttcgtggga taaagcccct cgcggggctt
      721 gtggtcaggc aaatttgaat atcagtgcca acacggcggc gatggcggta accagcccgg
      781 tggccgctat catgggatac cagcgggact cttgggctat ttttacggat tcggcgttga
      841 ttttgtgcgc gtcggcaatg attttggcga tttcggcatc tatttttctc agactggagt
      901 gtttcatttc ttgttcgatt atgttcatcg tacttccttt cgtttttggc ggttgccgcc
      961 gcttgtcgga tggtaggatg tctgccatgt gtatatattg atacctttta ggttttattg
     1021 caagtgtttt gggcggcggc ttcgtatgct tggcggtggc ggcggctgta ccattcggcg
     1081 agttcgcgcc gctcttggag gcggtagctg tcggcggtca ggtagtggtg caggcttgag
     1141 aagccggcgc gttggagggc ggcgcggctg atgtggccga gggcgaggtc tgttgtgagg
     1201 gtgtcttttc cggcggtcag gctgatgttg gtgaagaggt ggcggaatcc gtgcattgtg
     1261 tgtttggatt tgccgggggt gctgccgtca tagcccagtc gtcggatggc gttgtgggcg
     1321 aatttgatgc tgatgtggtc gggatggggg gcgggtttgc gccgtgggcg gatgccgggg
     1381 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1441 tccgacagta ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1501 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1561 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1621 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1681 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1741 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1801 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1861 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     1921 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     1981 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2041 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2101 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2161 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2221 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2281 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2341 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2401 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2461 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2521 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2581 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2641 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2701 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2761 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2821 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     2881 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     2941 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3001 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3061 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3121 agtcccagct tcttggcaat ttcgtgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3181 atcaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtc
     3241 tggccgtttt tggcaaaatt ttctttcaat tcgtctgccg taagcatttc tttggcctgt
     3301 acgcgggaaa atttcagtat ccgtccgtac ggttggttaa tacactcatg attaatcatc
     3361 gcttttctcc ttcatcccca gcaaaaccgc cgcttcgtgg cttttgccaa aattgccttt
     3421 cagcttgccg cgcaagaggt gctccaccgt ggtgcgctcc agattgaaat atttcgccca
     3481 atgcgccttg cacaccccgt tgcgcttaaa ccacccggcc gcgctctcgc gcgtttgcgg
     3541 ataggaaata ggcttgaaat tcaaaggttt ttccatatta tttatgcctt tcgtgtgata
     3601 taatgtgttt gtttttaggt ctctttgcac gataaacatc cggtctgttg tgcagcagca
     3661 gggtcttggc ttgttttaaa gtaaacaagc tttttctcgt agctgggtgt atgtgatcca
     3721 tcatgagctg tcgcgcatga gccttcaaag tgttcatttt ttcgtccttt ctcgtgatga
     3781 tttagggtgt ttgtgtttcg atgtggaaat tataggaaga attcttccta ttttgcaagg
     3841 aatatttatg aataactctt ccttttttgg caaccgattg aaagaagaaa gaaaaaaatt
     3901 aaaaatgact caagctgaaa tcgctgaaaa atgtggggtt tcaggaagaa tgtgggggga
     3961 ttatgaacgt ggcatcagcc agccaaaagc ggaacttttc ttccaatttg aaaaggtggg
     4021 tatagacgtt caatacgtca tgcacggcag acgcggcgaa acagcggtca tgccgtctga
     4081 aaccctgaac gccgaagaac aagaactgct ggtcttgttc cgcgaggcgg cagctgccga
     4141 ccgtgaaatg attctgatgg ttgcgcgcag ggcagagaaa aaagcccaaa ctgcgcttgg
     4201 taaagtgagt aatggataaa atggatcaat tcgaattgtg ccaaaaagaa catgtcaatc
     4261 catttgcctt gtcaaagcaa tatctattgg ttgtaacatt cgtcaaatcc agcagtaaaa
     4321 attttcaggc agcattactt tgggcaagaa gtgccaaatt atttgagaat cttgagattg
     4381 gaaaagaaac catctattgc tgcgctttcg ataaaacagc agaacaggct gggatggccg
     4441 gggtattttt gaattatatt gaaaattgga atggcaaaca gatttacatc aatggccgaa
     4501 tccatagtgg cagtatttat gatttgttag gggttttaga ctgctatcaa aaatcacagt
     4561 cctgtcccaa ccctaaaagc cactgttgct ttgtttcaga cgacattttt ctatggcatg
     4621 gatcaagacc aacgtttgaa atcagtcttg atctaactgg aaagaaaaaa gaaacatcct
     4681 ctgcaaagaa atttgtgatg ccttgtatta atttccgtca ccataggatt gaaaaagaaa
     4741 cctacttagg aaattggaat gaacaaattg ccgcattggc agtaaaacaa aatatagatt
     4801 ggtgtccaag ttttgatatt gagaatttta gacagtatga ataattacta tctatatagg
     4861 aattgcagca gcgatgtgtt atgggtcaaa cgtatccaac gccaaatcga cggcagccta
     4921 ctcttgattt ctgacaattc aacctatcca cccatgccct tggcactggc ggaacacccc
     4981 gatattcaaa tcatcgggca ggtagtgcag gtatcaaaag acttgaacta gacacaatca
     5041 aaaagggaaa tagaatgaaa atactcgctt tattaattgc cgctacctgt gctttatctg
     5101 cgtgtggcag ccaatctgaa gaacaaccgg catctgcaca accccaagag caggcacaat
     5161 ccgaattaaa aaccatgccg gtaagctata ccgactatca atcagcagcc aataaagggc
     5221 tgaatgacca aaaaaccggt ctgacccttc ctgaacatgt tgtccctatc gacaatgcgg
     5281 aaggaaagaa tctgctgcat gacttttcag acggcctcac aatcttaacc gttgataccg
     5341 ataaagccga caaaattact gctgtccgag tagtctggaa tacagatgca atgcctcaaa
     5401 aagcggaaaa actgtccaaa gctgccgcag ccttgattgc ggcaaccgct ccggaagacc
     5461 gcacaatgct gcgtgatacc ggcgaccaaa tcgaaatggc gattgacagc cataatgcgc
     5521 aaaaagagcc aacccgagaa tgggcgcgtg gtgggattgc ttataaagtc actgttacca
     5581 atttaccgag cgtggttttg acggcaaaag ctgagtaaat ctattaagta gaaaaaatag
     5641 aaagggaaat gatgattgag aaaagtattt ctattgtaga tggaaaggaa tactccgttt
     5701 ttgctgtatc acacgagttt cgttatacct ttgatgagcc tattttagtc gctgacttga
     5761 ttagttctct aaaagcttat gaaacactga caagtagtta tcttccagca attttgaatc
     5821 agctgtttga tgtcaaaatc caaaaaatca aagtagctgt atctgaaatt gaaagaggat
     5881 ctttccttga aaaactgatt ttcaatttat tcttcaaaga tgaagatgct tataatgaat
     5941 tttgtcttaa aatacgaaaa tttctaggaa cagaaaatca ggacggaagt attaatatgt
     6001 ccaaaatcat tatgtttgca atgactacac ttttaggggt aggtgctggt tatctcttgt
     6061 ttaaaaaccc gccacaagag aagcaggcaa taaccaacaa catcgttacc gtcattaatg
     6121 ctgatagttc tgtcgcactg gatggtgaac atttggtttc agtggtaaaa gaagtaacag
     6181 gaagcagcaa gcaaaaaact gcagaaaatg tggcaaaagt atatgctcca gcaagtaaaa
     6241 ataatggcag tattaccctt gggacagatg atgttcggat tgaacctgtt gcacaacaaa
     6301 ctgtagcaac tttgcctaaa gatgtggact tacgtgatac gccattgact gaagattaca
     6361 ccgatattga tgtgcaaatt cgtgctactg accgtgataa aaattcaggg tggtatgcag
     6421 tcatagacca aattgttcca tcacgtgttc gattagaact gcctgaagat attgatttga
     6481 ataggctggc taacaatgct actatccgtg caaatgtaac agttgagttt gacttaaagc
     6541 aaaatggctc tcgtaagcct aaaaaaatca tcctcacatc tctctctact gattaagttt
     6601 taacccgtat taaaggctta gtcagacggc ctttcctaca atccctgtat tgatttttaa
     6661 ttcaatacag ggatttttcc atgtcagaca agttcaacca attcatcaac cgcgtcctct
     6721 ctcacgaggg tggttacgcc aaccatccca aagaccccgg cggcgaaacc aattggggca
     6781 tcactaagcg caccgcacag gcaaacggct acaacggctc catgcgtgcc atgacgcgtg
     6841 aacaggcaat cagcatttac cgtaaagcgt tttgggagcg ttaccgcgcc gaccaaatgc
     6901 cggaagcggt cgcgttccaa ttttttgatg cctgcgtcaa ccacggttac ggcaatgccg
     6961 cccgtatgct gcaacgcgcc gcaggcgtac cggacgacgg cgttatcgga gcagtcagcc
     7021 tcaaagccat caattccctt cccgaaaacg accttttatt gcggttcaac gccgagcgtc
     7081 tggtctttta taccaagctc ggtacgttca cctctttcgg caagggctgg gtacgccgtg
     7141 tggcgcaaaa cctgattcac gcgtctgcag ataacactga ttaaagggag ataaaccatg
     7201 tcaaaaaagt cactcatcgc cctaatgacc gcagccatgc agcccgattt cagccacagc
     7261 gacctaggca ttcgctacgc catgccgact cagggatgtt ggacgcaagc ccaccgcaag
     7321 agcggggtag ccgccgcgaa acgcgcagcc aaaaaaaagc gtcacaaata accgcctttt
     7381 tccgatggct gggcggcttg gtctctaatc cggccacagg aaaaatcagc cataccaaac
     7441 tatgggcaaa cgtcgccgca gccgccatga cttggaagtt cgtgcaggcg gcggacgcgc
     7501 ccgaatggct ctagtgggct tatggcgcat tggtcggcgg gtatgcatta atcaaacgcg
     7561 gcatcgcggc gattccgcag ttggcagaaa tcaaaaaatc cgcaaatcag gaaggggggc
     7621 ggcaatgatt gaatttgtcc gagccaaaaa acggctgctt tgggcatttg tgcttttgct
     7681 tgtgtggacg tgcggttacc gatacgccgc cgacaaggcc gaagcgaaac aaaccgccct
     7741 gattgccacc tatcggcatt cttctatggt tgcggcggaa caatatgcct tgcagcttaa
     7801 aaaagcgcag gacgaaaggc agcggtggta cgacttttcc caaaaacaag gaagaaagcc
     7861 cgtgaaaaaa cagtatccgc cgcaaacgaa aaaagccggc tatctgaaaa ccaaggaaga
     7921 actgcttgcg gaattggctt gccttaaagc ggaaatggtt gccctaaaaa agcccgatgc
     7981 cttaatccat gggaaagaag tgcggcagaa agaacgcaac tcgtcgcagg gttaaggcaa
     8041 tgccatccgt tgaactgctg ttggagattg tccttctatt accaattggc cgtccaatcg
     8101 gcagaagaca aatatgccga tttgaaacgg catatccatg atatttatcg acgacataag
     8161 ggaagatacg gctaccggag gattgcggca gccatccgtc acgcaggaac accggtcaat
     8221 cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat acggcggcgc
     8281 aaataccgct cgttcaaagg agaagtcggc aaaattgcgc cgaatatcct gcgacgctgt
     8341 ttccatgcag aaaagccgaa tgagaaatgg gtaacggacg ttaccgagtt caatgtaggc
     8401 ggagaaaaga tatacctttc tccgattatg gatttgttta acggggaaat cgtcagttac
     8461 cgtattcaaa tccgcccgac tttcgatttg gccggcgaga tactgaaagg tgcgccggag
     8521 aaaccgggat cgtctgaaaa gccgatactg cattcggatc aaggttggca atatcagatg
     8581 ttttttatca aaagcagttg aaaggcaacg gtctggttca gagtatgtcc cgcaagggaa
     8641 actgcttgga caatgcggca atggaaagtt tcttcggaac gttgaaatcg gaatgtttcc
     8701 atacgtgcaa atatgattcc gttaccgaat cgtaagcggc actgcacgaa tatatccgtt
     8761 actacaacaa cgatagaatc aagttgaaat taaaaggact gagccctgtt cagtacagaa
     8821 ctcagtccct gaaagccgct tgattaaact gtccgacttt ttggggtcag ttcggcttcg
     8881 gcattttttt atccgttttg ggggtaactt gtttggaaag ctgcaagctt ataaataaag
     8941 gattacattt aagttttggg taaccttttt aaaaaaatgc gtgatgactt ttgcattttt
     9001 aaggcgtttt ttggggtaat tcgtgaaaag ttaccccaaa agttacccca taaatggcga
     9061 aaactcaagc atacgccagc atcctgcaac acaaaaaagc cttgaaactg ttgaagttca
     9121 aggctttttt gtgttgcagg atgctgctga aaatagggta tggtggaggc ggggggaatc
     9181 gaacccccgt ccgaaagtcc tctacaaagc gttctacata cttagttgtg tctatttgaa
     9241 aatcttattt ccatcatgcc gaccaacagg ccttttggaa accagttacc ttaagtctta
     9301 tttcctgcca agtaacccgg taggaaacca gtcaatgtaa gatgacgttg cggtggcttt
     9361 cgccacacag cccattgacc gactgctgca acggctagcc ttaagcggct aaagcgtaag
     9421 tttcgtcgtt tgcgactatt tgaattcagt gttttacggg aatctgagac cccggtatgc
     9481 ccgcatctgc ttcgcaaccc tcgtcgaaac caaggtcgcc cccagaaatg gtttgcaaat
     9541 tatacggata ttgtgcggtg ctgccaagtc tgtcggagaa atttgtcagt cttgctgcct
     9601 taatttgcgt ttgagcagga tgcggacgca gccgtcgttg ccttcccggg gttcggcgta
     9661 ggcgagtacg tcggggtgtt gcatcagcca gtttcgggtc atatttttca gaacgggttt
     9721 gtagcctttg gaacctaatc cgctgccgtg gatgatttcg ccgcatacgc cgcgtttttg
     9781 ggtgaatgcg atgaattcgt tgaggacttt ttgggcttct tcctgtgtgt agccgtgcag
     9841 gtcgacatcg gtaacgacgg gatagtatcc gtttttcagg cgttggatgt cgttttttcc
     9901 ctgtccgttt ttgctgaagc tggcgggcgg gtcgttgtat gtgctgccga tgtagaagta
     9961 gttttcttcg tcggcgcggt tgtctttggg acggactttg atgggggttt tgtcgggcgg
    10021 cgcataatat tgctgccggt tttttaatgg ggagagttgt ccgactgctt gtgaaaaatc
    10081 gaaatcctgt tcttgttttt gcttgttttt ttcggctgcc tgtttttctg ccgcttcttt
    10141 ttgggcttgt ttgcccagtt gtttgaggat gttttggaag tcggtattca tattttttcc
    10201 tgttatttgt ccgatggctg tttcgggcgg ggttttaatt tgccggaatg tttgccaatc
    10261 gggggaggat gattttgttg cctgcgtatg ttttttgaaa gtgtgattgt atatcaaaaa
    10321 gaaatgcggc aaccgtcggc agtgttgatt gccggaaatg cggaccggtc gaaccgatat
    10381 gcccgaacgc ctgataaagt tttaaaaacc tgccttgcga agcaggctga cgtgttttgc
    10441 caatcttgaa ttgccggaaa cgcgaaacac ggaaatctga
//
LOCUS       DAWISC010000058        10118 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWISC010000058 DAWISC010000000
VERSION     DAWISC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.058"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            328..1032
                     /locus_tag="WIW87_001048"
     CDS             328..1032
                     /locus_tag="WIW87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ5174220.1"
                     /translation="MLRLVLAASLSAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADIQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(1096..1662)
                     /locus_tag="WIW87_001049"
     CDS             complement(1096..1662)
                     /locus_tag="WIW87_001049"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ5174221.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(1728..2144)
                     /locus_tag="WIW87_001050"
     CDS             complement(1728..2144)
                     /locus_tag="WIW87_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174222.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGNVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(2206..3105)
                     /locus_tag="WIW87_001051"
     CDS             complement(2206..3105)
                     /locus_tag="WIW87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ5174223.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(3136..4593)
                     /gene="der"
                     /locus_tag="WIW87_001052"
     CDS             complement(3136..4593)
                     /gene="der"
                     /locus_tag="WIW87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ5174224.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     EKFPEPEAEEADARHPVFAVIGRPNVGKSTLVNAILGEERVITFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(4743..5372)
                     /locus_tag="WIW87_001053"
     CDS             complement(4743..5372)
                     /locus_tag="WIW87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ5174225.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RKVSQNQEAAAVLANIVEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WIW87_001054"
     CDS             complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WIW87_001054"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ5174226.1"
                     /translation="MAQKIQSVKGMNDLLPVKQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEDSQNHYVRFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTPTLQQWKNA"
     gene            complement(6762..7418)
                     /locus_tag="WIW87_001055"
     CDS             complement(6762..7418)
                     /locus_tag="WIW87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ5174227.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVIPKKAETISNKLFFTQHPKRQTEFTVGQIRQARAE
                     "
     gene            complement(7476..7667)
                     /locus_tag="WIW87_001056"
     CDS             complement(7476..7667)
                     /locus_tag="WIW87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174228.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(7700..8236)
                     /locus_tag="WIW87_001057"
     CDS             complement(7700..8236)
                     /locus_tag="WIW87_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174229.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(8404..8604)
                     /locus_tag="WIW87_001058"
     CDS             complement(8404..8604)
                     /locus_tag="WIW87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174230.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTRA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(8719..9138)
                     /locus_tag="WIW87_001059"
     CDS             complement(8719..9138)
                     /locus_tag="WIW87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174231.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(9196..9561)
                     /locus_tag="WIW87_001060"
     CDS             complement(9196..9561)
                     /locus_tag="WIW87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174232.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(9543..10091)
                     /locus_tag="WIW87_001061"
     CDS             complement(9543..10091)
                     /locus_tag="WIW87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174233.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
BASE COUNT         2515 a         2402 c         2372 g         2829 t
ORIGIN      
        1 tgcaaaaccg taagggtcgg tcaggatgct tgctgtttct ttccgcaagt agcggaacat
       61 cggtgcaagc acggcggttt tgatgccttt cctctgcaat gccagtgcct gattttgaaa
      121 aaaatcccgt ccacatcctg ttcggattgc ggataccatg aggggatgac gaggacgtgc
      181 aagggttcgg gcatagtggg attccgtatc ggaaaggcgg ttattataag acagacgcag
      241 accgaatatt taaattgttg ccttacgcta atgcaatttg gcgcgccggt gtgttagatt
      301 ggcagtttta tcggtaagga ggcggatatg ttgcgtcttg ttttggcggc ttcgctgtcg
      361 gcggtatctt ttccggcagc ggctgaagca ttgaattaca atattgtcga attttccgaa
      421 tcggcgggtg tcgaggtggc tcaggataca atgtccgcac gtttccaagt gacggcggaa
      481 ggacgggaca aaaatgccgt caatgctgag tttgttaaaa aattcaacaa gttcatcaga
      541 aaatcgaaaa atggtagctt taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctat
      601 caatatacca acggcagacg cattcaaaca ggctgggagg agcgtgcgga atttaaggtc
      661 gaaggtagag attttgatga gttaaaccgt tttattgccg atattcaagc agatgccgcg
      721 ttggaatata cggatttcca tgtgtcgcgc gaacgccgca acgaggtcat cgatcaggtc
      781 agcaaggatg ccgttttgcg tttcaaggcg cgtgccgaaa agttggcggg cgttttgggt
      841 gcgtccggtt ataaaatcgt caaattgaat ttgggacaca tcggcagcca tatcgcggga
      901 gggggagctg ctcaggcaaa aatgcttcgt gccatgccga tggcggcaag cgtcaatatg
      961 gagggtgcgg attccgccgc gcctggtgtg gaggaaatca gcatcagcgt caatgggacg
     1021 gttcagttct gatttgaggt gaacggcaaa tgccgtctga aacccgacga taagggttca
     1081 gacggcattt atatttcagg ctttgggcag ggtaacgccg gtttgcccca tatatttgcc
     1141 gttgcggtct ttgtatgagg tttcgcagat ttcgtcgctc tcgaagaaga ggacttgcgc
     1201 cacgccttcg cctgcgtaga ttttggcggg caggggggtg gtgttggaaa attcgagggt
     1261 aacgtagcct tcccattccg gttcgaacgg ggtaacgttg acgataatgc cgcagcgggc
     1321 gtaggtggat ttgcccaagc agacggtcag gacgttgcgc gggatgcgga aatattcgac
     1381 cgtgcgcgcc agtgcgaagg aattgggcgg gatgatgcag cagtcgtctt caacggtaac
     1441 gaagtttttc gggtcgaagt ttttgggatc gacgatggtg ctgttgatgt tggtgaagat
     1501 tttaaattcg tttgcgcagc ggatgtcgta gccgtagctg gacgtaccgt aggagatgat
     1561 gcgtttgccg tcggcttctt tgatttggtt cggctcgaaa gggtcgatca tgccgaattc
     1621 ttcgctcatt cggcgtatcc atttgtcgga cttgatgctc ataatgtttt ccttgtttct
     1681 tgcagtgttc ggacaaagca ttgggggatg ccgtctgaaa acggggctta tttgtttttg
     1741 ggcagtttca cttctttaat catgccgttt tcgcatttca taatgaagag ggtttcgccg
     1801 ttttgcgaga cggtaaatct gccgtgtgcc aagccttttt tgaacgtgcc cgagagtacc
     1861 atgttgcgga atttggtact gtcggaattg aacggttcga taaatatttc gcggttggcg
     1921 gcaacggtat aaacgccttg cccgtcgaat ttgccgtttt taaacgaacc ggtatagttg
     1981 cgcccgtctt ggcagcgcca tgtgcctttg ccggcgggtt taccgtcttt gccgacattg
     2041 ccgtcgtagg tgcagcctgg ttcttgatag gaagtcagga cggcggccga agtggggagg
     2101 gcgaacatca tggcgggcag taggaatgcg agatgtttaa gcataagggt tattccattg
     2161 gattttggtt gacggtattt tgtcgtgaaa aagccgtctg aaaaatcaat cttgccagcc
     2221 gcccaaatag gaaaccagtt cttccaacat ggtgctgacg gattccgcca tcagaatttg
     2281 cgaggcgaag gcaaggccgg cggcatcgtc gccgttgctt tcggcttctt cctgcaatac
     2341 gtcgaggtat tggatgcgct tgagtgtgaa gtcttgagtg aggataaagg caatttgttc
     2401 gcgccacacc aagcccagtt gggtaacggt tttaccgttt ttgacgtgtt gaaccacttc
     2461 gtcggcggtt aaatcttgtt tggatacttt gacgacggga acaatatcgc ccgtaccttt
     2521 gagttcgcaa tcgctgtcta attcaaaacc gccttcgcaa tgcccttgca acagccagcc
     2581 ggtcatcaag gaagagggcg attgcttggt attcggcagc gaggcttcca aaccgcccaa
     2641 agcttcgcgc agcttggtca ggatgttttc tgctttggcg gaagccgcgt tattgacgag
     2701 caggtagccg tggcgggtgt taaacaccgc ttctgtacgg ctgctgcggg taaacgctcg
     2761 gggcagcagg tcgtctgtaa tttgctcttt aagctcttgt ttttctttac ggccgacatt
     2821 gcgggcttca ttgttttgga tttccgctac cttctcttcc aaaatatcgc ggatgacgcc
     2881 ggcaggcagg actttttctt cttttttcag ggcgacgcgc aaggtaaagt cggcagggaa
     2941 aacgagttca ggggagaatg aaaccggtgc ggtaaagcct tcgctgaacc agtctaagcc
     3001 ttggcaatgg gtaaattcag cttcagcaag tttgtcggca agtacgtctg cctcaggcag
     3061 cttttctttg ttgagcggat aaaaactaat ctgcttgaac cacataatgt ttcctattgt
     3121 ttgaaatgtc gggaattatt tgctgaattg ttttttcaca ctgactttgg ttttcttctt
     3181 gaagcggttt ttctcttcct ttcggccttc gcgtttctca atacggttgc tcaggctgac
     3241 gcggcgcagc ggttttttct tcggtttgtc ttccgcattt tcatacgggt tttccgaaac
     3301 attgtattga attctgagcg gcgtgccttg cagattgaag gctttgcgga acgtttgggt
     3361 cagatagcgc gtatagctgt cggaaatcgc gtgcagcgaa ttgccgtgta ccacaattac
     3421 gggagggttc atgccgcctt ggtgggcata acgcattttc ggacgcacca agccggcacg
     3481 cggcggttgc tgacgctcga tcgcgctttg taatacgcgc gtgattttcg gcgtcggcat
     3541 cttaatcatc gccgcgttgt aggcagcctg aatgctgtca aacaaaccgt ctataccgcg
     3601 ctctttcaat gcggaaataa agtggaactt ggcaaaatcg aggaaataca gtttgcggtt
     3661 gatatcgcgt ttcacttgct cgcgacgttc ttcgctgatg ccgtcccatt tattgaccgc
     3721 caccaccaaa gcacggcctg cctccaaagc gaaacctgca atcgtcgcat cttggtcggc
     3781 gatgtcctgc tgcgcgtcca ataccaaaac agcgacgttt gccgcttcaa ccgcctgcat
     3841 cgctttgata acggagaatt tttccactgc ttcatccact ttgccgcgac ggcgcacacc
     3901 tgcggtatcg atgatggtaa acggtttgcc ttcgcgctcg aaatcgatat ggatactgtc
     3961 gcgcgtcgta cctgccatat cgaaggtgat gacgcgctct tcgccgagaa tggcgttaac
     4021 cagcgtagat ttgccgacgt ttggacgacc gataacggca aaaacaggat gtcttgcatc
     4081 ggcttcttcg gcttccggct cggggaattt ttccaaaata tcttcaatca gataatacac
     4141 accatcgccg tgtgcacctg aaataacata agggtcgccc aaagcaagtt cgtagaactc
     4201 ggcggcaagt acagccctat tgcccccctc gcctttattc acggccaaat aaacagggcg
     4261 cggactttgg cgcaaacggt cggcaataat cttgtcttgc ggtgttaaac cggtacggcc
     4321 gtccaccaaa aacacaactg catcagcttc atcgacagcc tgtaaggttt gttttgccat
     4381 ttcgtgcaaa atgccgctgt ccacaaccgg ctcgaaaccg ccggtatcga tgaccaaata
     4441 aggtttgctg ccgactttgc cgtgtccgta atggcggtcg cgcgtcagac cgggcaggtc
     4501 atgcacgagc gcgtctttgg tgcgcgtcaa acggttgaat aaagtagatt tgccgacgtt
     4561 ggggcgacca acaagcgcga tggttggttt catgattgag tctttctgtg tcaagtgccg
     4621 ttcgggagaa ctgaacacga gcaggtgtcc gttggacacg gcggatgggt tttacgggaa
     4681 ttgccgtagg atagtgttgt ctgaaatgcc gtctgaagag agggtggcat ttcagacggc
     4741 atttatttca gcgaatcaag tttcatttga accaattcgc gaccgacaga atcttgaggc
     4801 attttttcta aagcctgtcc gtagtttttt aaggcttcct ggctttttcc ctgtgcggca
     4861 tagacatcgc ctttggtttc catcagcagg ggggcgaagt ccgcttcaac cggcgtatcg
     4921 agcgcggcaa gcgcggcatc gtattttttt tgttgcaaca acacaacgcc cagacgctgc
     4981 gccgccaacg cttgaatcag gctgtctttt tggttggaca acacccattt caaatggcct
     5041 tcggcaacat cgtaacgctg cgcgtcaaat tcggttgccg ccgccatcag tgtggcttgg
     5101 gcggcggaaa tggaatgcgg gtagctttgt tggagtttgg tcaattcggc attgatttcg
     5161 ctttgcgggg ctttgctttg cgccttttct acgatgtttg ccagcaccgc cgccgcttcc
     5221 tgattttggg aaactttacg gttttggtaa accgtgtatc ccaagtagcc gagtgccgcc
     5281 aaaatcagca aggcaaacag ccatttgccc gtggttttcc aaaaatattt aaagttgtct
     5341 aactcttgtt gttcttcgag atgggctgcc atttatgcgt tcttccattg ttgtaaagta
     5401 ggggttaaat cctcggcggc gacagtttgc tgaccgtgtg cgccgttcat gtctttgagc
     5461 gtaaccgtac cgttcgccag ttcgtcttgc gcgacaatca gggcaaagcg tgcgccgctg
     5521 ttgtcggctt ttttcatttg cgctttcagg ctttgatagc cggaatgctg cattacattg
     5581 aaaccttgcg cgcgcaaggc ttgtgcgtat ttcatcacct gcaagtccgc cccttcgcct
     5641 tggtgcattg catagacatc aggcgcagcg ttcacttcca gagagccgta ttcgctcacc
     5701 aaaagcagca gccgctcgat acccattgca aagccgatag acggcgcagg tttgccgccg
     5761 agttcttcaa tcaagccatc gtaacggccg ccgccgcaca cagtcgcctg cgcgccgagt
     5821 ttgtcggtcg tccactcaaa aaccgtctga ttgtaataat ccaaaccgcg aaccaagcgc
     5881 ggattttcaa tatattggat acccaaacca tccaacatcg ccttgaagcg tacatagtgg
     5941 ttttgcgaat cctcgcccaa gtaatccacc aaacgcggcg ccgcgttgca gatttcctgc
     6001 aaatctgggt ttttcgtatc caaaacgcgc aaaggattgg ttttcagacg gcgtttgctg
     6061 tcttcatcca atttatcttc ataacgggtc agatattcaa ccaatgccgc acggtgtgcc
     6121 gcgcgttcct cacggttgcc caagctgttg atttccaaag tcaggtattc gcggataccc
     6181 aatttttccc ataagtcggc agacatcgcg atgatttccg catcaatatc cggcccttca
     6241 aaacccaaag cctcgatacc gacctgatgg aactgacgat aacggccttt ttgcggacgc
     6301 tcgcggcgga acatcggccc catataccac agcttttgcg ggctattgta cagaaggttg
     6361 tgttcgacca ccgcacgcag gcaggaagcc gtaccttcag gacgcaagct caaactcaaa
     6421 gaatcgtttg aatcggagaa ggtgtacatt tccttgccga ccacatcggt ttcctcgccg
     6481 atggagcgga caaacaaacc ggtttgctcg acaatcggcg tacggatttg ctgataaccg
     6541 taagcgcgtg tccagcggcc gaccgtatct tcaaacgcct gccaaaacgc agccgtcagt
     6601 ttgaaatctt tttgcttgac aggcagaagg tcgttcatgc ctttgacgga ttggattttt
     6661 tgtgccattt caagtaagaa tgcttaaatc aaattgcggg cgattatagc ggattttaaa
     6721 gggtttgtga ggttggaggt ggtttgcgga cggcatttga cttactctgc acgtgcttgc
     6781 ctgatttgtc cgactgtaaa ctccgtctgc cgttttgggt gttgtgtgaa aaacaattta
     6841 tttgaaattg tctcggcttt tttcggtatg acagccaaaa tcttacctgc caaatttccc
     6901 tcacgggttt gccaagcatc caaaaactgc gccctgctca ttgaaacatg ccccagcgac
     6961 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgaaaa atggtcgtct
     7021 ttgcggtatt tcagatacac gatgacgggg attttcaact gcgcgagctg ctcgaaagac
     7081 agggcatagc ccttcgcctc aaaacccaaa tcaggcataa tgcgccgcat atcctcaaac
     7141 gacgcgcgca tctgctcctt atccagtttt tccaacactt cttcttccgt cagcttttgc
     7201 ccgtaaaaat tgttcaaaag tgtcgccacc gaagccgccc cgcaggaaaa atccaaatcc
     7261 tgctttacaa tattgaaatc ccgcctttct ttccaactct gcactttgat ttttccgtaa
     7321 acaacaggat tatcgttaaa catcggtgca gcattcaaac gataagataa agaaacgaca
     7381 acacacgcca acagaaaaac atatttgaac ttcatcatat tgtccacata aagggcagcc
     7441 tgaaaatctt tcaggctgcc cttgtcaaat tattcctagc tttcggcttt tttggcaaac
     7501 caaacaatcc gattacccgc ataatacttt ccatttattg aaatccgaca agccgcgccc
     7561 aaaaaatgcc atgcactgtc gatttccgca gcaatctttg taccgttttc ttcaaattcc
     7621 aaatattcac ccaataataa acttgaaaca gaacgcgtgg gcagcaagtg cccaccctac
     7681 gcttattcaa ataatttgat taaataaaga ataaagaaaa tgcatcagga acaaaattat
     7741 aatctgccac ctgactcaca ccgcttttaa agtaaggggc atcaaaatca aaaccgcaaa
     7801 aaaaataatt tttgcattga tttttaatag atttaaaatt cgaatatagt gtttctccag
     7861 atttaaaaag tttatcttta tctatatatt tgatgctttc cctatccaaa ataaatattt
     7921 caaacattaa aaaatcatta catgaccaag ccaaatcata aatttgattc aaatgggtat
     7981 cataaagata aaaataataa ggtttgggaa caggtaaaat atttaatgga aaaggaggac
     8041 taattttctt aatatctgat gactgatatc catatctttc tatattctta aaaatttcat
     8101 ctttcttaag ataacaagac attctgacaa tcacaacctg ttcatcggat atattatcta
     8161 tttgcatggc gcgtataaca cgccatgcct gattaaaatt agtctccctt accttagaat
     8221 ctattatctt tttcaaagat gaaaatgccc cttatcttga actctccagt tagaacctgg
     8281 aagtttcgta cctcttaaat gtgtattaat atttcttata gtttcattaa aatgccacgc
     8341 gctgccgatt tcaacggtaa ttttcgtacc gctttcttca aattccaggt attcccccat
     8401 tagctaacgc aaagaagcag acgccatttc ggcttcgtta tgataaaccc gccttccgtt
     8461 gatatagact tccgcccttg tccgctccaa attccaaaaa ttccgtactg aaatttccat
     8521 atcccgatat tgtgcagacc atgttttttc gaaggttttc ataaaatttc ctatacctgt
     8581 ccaatcggca catatcaatt gcattattat agtggattaa caaaaatcag gacaaggcaa
     8641 cgaagccgca gacagtacaa atagtacaga accgatttac ttggtgtttc agcaccttag
     8701 agaatcgttc tctttgagct aaggtgaggc aacgccgtac tggtttaaat ttaatccgct
     8761 atacatctca atacgataaa tattccttaa gtcaaaatgc aagcctgacc gtaccttaac
     8821 tgtcaaaatt ttattatttt ttattgattt taaaacaatt tctgcaaaat tctcttcgct
     8881 ttctctcttt tttagaagca cataagaaaa aataaaactt ccccgattaa attcataaat
     8941 atgtttcaac cattcgcctc ctctttctgt aagacaagat tcagtttcat tcttccttat
     9001 tgtataaata tttccttcac aaaatctgaa ataaatccat aaatccatct tatccataat
     9061 taaagaaaaa gtttcacctc gagattttgt caacaattcg caaggttgcg atgttgcaat
     9121 caaatagccg aaagacattt tttacctcat acatggtcga aatcagtttc tgttagttca
     9181 gaatccattt tttcgtcaac aactgaatcc gcatttttga attaacgttt tcatcagctg
     9241 ccgtttatct aaaccggcag gttcagtttc aaaataagcc ttatatgaag actgtaagca
     9301 tttcagaaaa agatcatcag aagacatatc tgccgaatca aatacaactg ttttgatttt
     9361 ggtacttacc caaaaccctt tttgctcttt ttctactata cggaaattca gaatatttcc
     9421 aaccgaatca aaagcacggt aaacatcatc catcaaatcc tgcggctcta ttttcttttc
     9481 caattccgac aatccttgaa atatatccaa agacacatct tcaaatagaa aaaaaggagg
     9541 agttagaagc ggtttttcca tgatctgtcc gtagattttg attcccaagg gcgatgacga
     9601 ccaattccct gtccaggcaa agtcttgccc gtattatccg taactcgacg atgataatgg
     9661 ggaaattttc caataggatg acctgttcta ttaccgaaag gggctatccg catattattg
     9721 ccgattttaa tctcacgtcc atatttagca aaggaaacaa cctttcctgc ggcgcctaca
     9781 ccaccaggaa ttgcgcctaa tccgccagca atagcaacat ctctaacaga agctggtctg
     9841 cctgtcgttg cataactaaa accatgctgt gtccacatac caatggcagc accacccaag
     9901 atagccaatg gaagaaacgc cccctcagtc tccttcatct ccttctgaga aagctccgcc
     9961 aactgcatcg gcgcatctgc ccgcgtatgg aatacttggt tttcaaatat ctgattgtcc
    10021 atgctgtttg ccatcgccgg agcggcaatc attgtcagaa tcatcatcgc tgcggtaatt
    10081 tgttttttca taataactcc tttggattac aaggttgg
//
LOCUS       DAWISC010000059        10086 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWISC010000059 DAWISC010000000
VERSION     DAWISC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.059"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="WIW87_001062"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="WIW87_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ5174234.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRLLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WIW87_001063"
     CDS             complement(1966..2568)
                     /locus_tag="WIW87_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ5174235.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WIW87_001064"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WIW87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ5174236.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WIW87_001065"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WIW87_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ5174237.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WIW87_001066"
     CDS             complement(4526..5488)
                     /locus_tag="WIW87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ5174238.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WIW87_001067"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WIW87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ5174239.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEVRAILTEE"
     gene            complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WIW87_001068"
     CDS             complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WIW87_001068"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ5174240.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7044..7520)
                     /locus_tag="WIW87_001069"
     CDS             complement(7044..7520)
                     /locus_tag="WIW87_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ5174241.1"
                     /translation="MEHLFGKWLPDLPAAISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVAGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WIW87_001070"
     CDS             complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WIW87_001070"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ5174242.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WIW87_001071"
     CDS             complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WIW87_001071"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ5174243.1"
                     /translation="MPSEHQHISSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLNRIFYADSGSISVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIMLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            9697..9812
                     /locus_tag="WIW87_001072"
                     /pseudo
     CDS             9697..9812
                     /locus_tag="WIW87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2334 a         2747 c         2568 g         2437 t
ORIGIN      
        1 gcacaatcac ttgttcaagt gattaagaaa gcgtgataat acggatttat ctgagttctg
       61 tctattgttt tagactttta tttttagtta ttttttaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaatgac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgg atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tggggaaggt gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg caactttatt cagcagacgg ggcggattgc ctttgttggc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag gcaacaggac
      601 gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg tttcgccgct
      661 caaacgctct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcggcggcag cttgcaggct
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcaggct cgatccggta
      841 tttccgcgcc atccccatca gctcgcccat gcgctgctct tgtgcggcaa gttcgttggg
      901 attgatgtcg ctgcgacctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ttcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcag cctgcaacag ctcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgagg gcttcccact cgccttgttt aatgtccaac tgattcaatt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc aattttgata aagctgcctg acggtttccg cctgctccct
     1321 gctacccgca aatgcgtcca acaattcgcg ctgggcggct tcctgattaa gcgaatgatg
     1381 ggcgttttgc ccgtggatgt cgataagctg gctaccgacg gctttgagtt gcgccaaggt
     1441 agcggcctga ttgttgataa agctgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tcttctccgt cgtttaaaag cccctgttca tacaattctg cttttaaaac
     1561 aggtaaatgg gaaatatcaa acaacgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggctttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgcca gtttctccgg tcaatacggt aaagccgctt tgaaaatcca gattcagatt
     1741 ttcaacaatg acaaaatcac gcaaagaaag tgttagaagc atcgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttataactaa gattttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaagctgt tcaaacccaa
     2101 atcgctgagg aacgttgccg ttgtcgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt atgccctcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca gttcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttacgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccgattttg ccttcacgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcaaca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca acgcgttgcg tccgactgcg ccgccggcgg
     3061 caaggttgta gccgccttct tcgacgatga ttttcggcca tagcattccg ggggtgtcgt
     3121 agagccagaa gtcatcggcg aggaagaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcagatgagg acgcgcaggg gtttatctat gccttggcgg tggggaatca
     3301 tggcacgaca ggcttgggta attttgccgt gtgcgcctgt ttcggaggaa tcgagggcga
     3361 tggcgcaggt gtcggggcgg ctgttatagt gttcgagcca gattttggtg cgctcggggt
     3421 cggcaagatc ttgtttgttg aggattttaa gtttgggttt acctttggaa agctgggcaa
     3481 gcagggggtt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgctttttgc acgctcggcg atggcttttt tcgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag gctgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tgctcagagg atgtcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcaatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggacttgccc gaaccggaca ggccgctaat caggacgatc ttcattgtgt tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc gaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc aagctgcttc atatactcgt cgtccgcctc gactaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca ggccggaatg cccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccaag gtgcgttgca
     5101 ggtaaatccg caacacgtcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atgacaatag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 taaccaaaga catagaaata tcgaacaggt cgccaaactg ataacccgtt tcccccgatt
     5281 cgaggcggtt cagatattcc gactctgcca aaccgaccac ttggatttgg ttgggatgaa
     5341 tgaaattcag gtgtccgact agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccaag cgagttgcag tttgtattgg ttgtcatcaa
     5461 acaggcggcg gacggagata ctgggcatat tattcttcag tcaggatggc acggacttct
     5521 tccgcagagg aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc
     5581 agtttggata agacttccaa atgctcgccg gttgcgtttt ccggaaccag caagataaaa
     5641 atcagggaaa ccggcttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 gacaatgagg attcgcgttc caaaagcagg cctgcttcct caaacagcct ttttttactg
     5881 cctacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccgg ggcaaatctc
     6001 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6061 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6121 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6181 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6241 ttggcggtat tgcagcagca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6301 gataaccgca cccaatactg ccatcagcag ggtaaagcct ccgtgacaca gcataacggc
     6361 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6421 ttctttgtcc gccattgcat aaaccgtgtc atacgccaga gtccataaca cattggcggc
     6481 aaagagtatc cacgcttgag gcggcacgtt tccggcaacg gcggcaaacg ccatcgggat
     6541 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6601 cgggtaagtc agcgcaagaa acagcgcggg caggctcatc agccaagtca gatgattcag
     6661 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gcgcttcttt
     6721 tttcttgacc ctgccctgtg cgaacggacg gttttttgta cgctcgacag caccgtcaaa
     6781 atcgcggtcg gcaaagtcgt tgatgacgca gccggcacta cgcattaaaa acgtgccgat
     6841 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     6901 gtaggtcggc cacagtaaaa gcagcgtccc aatgggcttg tccgcccgca tcaggcgcag
     6961 gtacacatcc aaacggtcgg acaggcgtaa aaataaaggg gatttaggat tcatattgcc
     7021 gcgcagcttg aaaaaacggt attttatccg ataaaacgtt tcagttcggg cagaaaatac
     7081 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7141 cgtccgcatc cttcgccggc aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7201 gcttgaaaca gacgctcgcc caaaggacgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7261 gccgaaccga tacggcattc gctccttgcc tcaacaacag ggatacggtc cagcttcaac
     7321 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7381 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggtgtgcagc
     7441 agccgcacca tcggcaggct gatgccgtct gaaatggcgg cgggcaagtc gggcagccat
     7501 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7561 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7621 cttgggcgat gtgtgcgtcg cggctgtcgt ggatgtggtt gaacacgagg ctgtgcaagc
     7681 gaatgccgta ttgtttgagc gcggcgaaac tgagtaaagt gtggttgata ctgccgagcc
     7741 gtccgctggt aacgaggatg acgggatagc cttgctgacg gatataatca atggttaaca
     7801 ggttttccgt cagcggaacc atcaatccgc ccgcgccttc gaccaaaacg acttcgtact
     7861 gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaagcc ctgccatcca
     7921 gtcgggcggc gaggtgaggc gaagcgggat agctgaagat ttcgggcata gtcagccgcc
     7981 gtttgtcggc ttcctgcatc ggtatgccca taattttgcg gtggacggcg atgtcgtcgg
     8041 caatgttttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8101 acaattgttt tgccaacacg ccggtggcga cggttttgcc gatgtccgtg tctatgccgc
     8161 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8221 aagtttggtc aaaacgccgt ctgaaatgat atagggcggc atcagataca ccagcctgcc
     8281 gaacgggcgc acccaaatgc cctgcgccac gcagtccgct tgaaaacgcg ccatatccac
     8341 gcctttttcc agctcgatca ccccgatggc acctaaaacg cgcacgtctt tcacgccgcg
     8401 aatgtcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8461 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8521 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8581 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8641 gccaatacac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8701 gcgcccgaat ccagtggcga tttcgtcaaa aatcagcatg atatcaaatt cgtcgcacaa
     8761 atcgtgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     8821 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaata aggcgcggac
     8881 aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg cttttcggat tatcgacaaa
     8941 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9001 ggacatcgcg ttccaagtat cgccgtgata cccgcggcgc accgtcgcga tattctgctt
     9061 cgccgtcaaa ccccgcgcct gctggtattg cactgccatc ttcagcgcaa cttccaccga
     9121 aatcgaaccc gaatccgcat aaaaaatacg gttcagcccc tgcggcaaaa tcccgaccaa
     9181 caacttgccc agctccaccg ctggctcgtg cgtcaaacca ccgaacatca cgtgcgccat
     9241 ttgtttcatc tgcgtctcaa ccgcctgatt caaaacagga tgattgtagc cgtgtatcgc
     9301 acaccaccag gaggacatcc cgtcaatcag ccgcgtgccg tccgccaatt cgataaacac
     9361 cccttctgca cgtttgacag gataaacggg cagcggatcg gtcatggaag tatagggatg
     9421 aagcagatgg gtacggtcga aatcaagcaa tgatgatatg tgttgatgtt cagacggcat
     9481 aagtttctct cttttcttct tactgtattc aaacgcaaaa cgcgtattct actccgacag
     9541 accgtttccc acacctctcc atccgtttcg ggcgcaaaac cgcgaaacaa atcgtccgca
     9601 gtataagcgc acaccgtttc gcattcccca agcccgattg gaatcagacg gcccaacgcc
     9661 caataccgtt tcacgcagcc tccgcccaac gcttcaatca gtcatagata tagtggatta
     9721 acaaaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     9781 taacgccgta ctggtttaaa tttaatccac tataaaacgg caatccatac gatacagatc
     9841 atagcaacag ccatcgcaac agcgttagca aaatcagggg actccgacat aggcgcatag
     9901 cacctaccga tgcacggctc ctcattcggc tctatgaata ccatacccat cacaaaatcc
     9961 accgccaaaa ccaggcacgg cttcttatac ttatgataga tttccaccat cctgtcccat
    10021 atataccaaa cattcatacc gtatatcccg caggcaacaa attccgattg aaggttacag
    10081 ccctat
//
LOCUS       DAWISC010000060        10072 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWISC010000060 DAWISC010000000
VERSION     DAWISC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.060"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..126
                     /locus_tag="WIW87_001073"
     CDS             <1..126
                     /locus_tag="WIW87_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5174244.1"
                     /translation="IVFEPFFISNDTDLALFKTTKWGICRAIADAIAMELGAAKV"
     gene            123..326
                     /locus_tag="WIW87_001074"
     CDS             123..326
                     /locus_tag="WIW87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174245.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNRR"
     gene            329..493
                     /locus_tag="WIW87_001075"
     CDS             329..493
                     /locus_tag="WIW87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174246.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            496..732
                     /locus_tag="WIW87_001076"
     CDS             496..732
                     /locus_tag="WIW87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174247.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            704..1123
                     /locus_tag="WIW87_001077"
     CDS             704..1123
                     /locus_tag="WIW87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174248.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMERDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNASGGCIDGFGSHGLQLYNRALGYGN"
     gene            1143..1325
                     /locus_tag="WIW87_001078"
     CDS             1143..1325
                     /locus_tag="WIW87_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174249.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(1370..1540)
                     /locus_tag="WIW87_001079"
     CDS             complement(1370..1540)
                     /locus_tag="WIW87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ5174250.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1553..1771
                     /locus_tag="WIW87_001080"
     CDS             1553..1771
                     /locus_tag="WIW87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174251.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1768..2106
                     /locus_tag="WIW87_001081"
     CDS             1768..2106
                     /locus_tag="WIW87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174252.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            2118..2384
                     /locus_tag="WIW87_001082"
     CDS             2118..2384
                     /locus_tag="WIW87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174253.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            2381..2578
                     /locus_tag="WIW87_001083"
     CDS             2381..2578
                     /locus_tag="WIW87_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174254.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2581..3087
                     /locus_tag="WIW87_001084"
     CDS             2581..3087
                     /locus_tag="WIW87_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ5174255.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(3084..3674)
                     /locus_tag="WIW87_001085"
     CDS             complement(3084..3674)
                     /locus_tag="WIW87_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174256.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALIAAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            3687..5309
                     /gene="terL"
                     /locus_tag="WIW87_001086"
     CDS             3687..5309
                     /gene="terL"
                     /locus_tag="WIW87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ5174257.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            5309..6877
                     /locus_tag="WIW87_001087"
     CDS             5309..6877
                     /locus_tag="WIW87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ5174258.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            6864..8159
                     /locus_tag="WIW87_001088"
     CDS             6864..8159
                     /locus_tag="WIW87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ5174259.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            8269..8684
                     /locus_tag="WIW87_001089"
                     /pseudo
     CDS             8269..8684
                     /locus_tag="WIW87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
     gene            8915..9976
                     /locus_tag="WIW87_001090"
     CDS             8915..9976
                     /locus_tag="WIW87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174260.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2650 a         2352 c         2786 g         2284 t
ORIGIN      
        1 attgtgtttg agcctttttt catcagcaac gacactgatt tggccttgtt taagacgacc
       61 aaatggggca tctgccgcgc gattgcggac gcgattgcga tggaattggg agcggcgaag
      121 gtatgaaaaa gtctttgatt gctttatgtg ttgcccattg tgcaaagttg aaaaacgatt
      181 ttggcgtacc accgttacct gaaatcaaaa tcacgccaag ccctgttcgg gtaggctctt
      241 tgaaacaaca tccgagcctg cgcttgggta aatcaggcgt ggcggctgct aaacgtgcgg
      301 cgcgcaaacg caagaatcgt cgttaatcat gggacaggtt gcgttttacg aaaagatgat
      361 tgggctgtgg tcggccaaaa gccgtgaggc aagcgaacag gcggacttgg ctgcgtttga
      421 atttgcggag ggcgaactgg ccaattatcg ggaaatgctg aaacggcacc tgcaaaccaa
      481 aagtgtggaa tagcaatgcg tattttggat atttttaaaa acccggcgac aggcaatgtg
      541 tcgcactcga aactgtgggc aaacgttgcc tgcgcggctg ggacgtttaa gtttgtgatg
      601 ttgcccgatc cgtcggcgga aatttgggcg gtgtatttgg gcattgtcgg cggctatgcg
      661 gtggcgcgtt catttgtcag cgtgaagcgt caggaggtcg agaatgaatc tcgtgaaact
      721 gctggcgaat aactggcaac cgattgccat tatcgcgctt gtcggcacgg gcttggctgt
      781 gtcgcaccat caaggctaca agtcggcatt tgcgaagcag caggcggtca tcgacaagat
      841 ggagcgcgac aaggcgcaag ccctgctgtt gtcggctcaa aactatgcgc gcgaactgga
      901 actggcacgc gcggaagcta aaaaatatga agtcaaggcg cacgctgtcg gcatggcttt
      961 ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa aataaaaagg aaatcgaaaa
     1021 tgtccttact caagaccgta aaaatgcaag cggcggttgc attgacggct ttggctctca
     1081 cggcctgcag ctctacaacc gcgccctcgg ctacggaaat taaggttgtc gaaaaggcag
     1141 tcatgccgac cccgcctgct gcattgatgg tcgcgccggt acgcccgaat ccgccgaaag
     1201 acggcaagac ggccacgctg ttggaacacg ccgctgagtt tggtggctat gtggcggagt
     1261 tggaaaatca aaatcaggct tggcgcgact gggcgggcaa tcactcccgc aaagtcggaa
     1321 actgacaaaa aagcccgcgt agggcgcggg ctgagggtga aagcggattt tatacctctt
     1381 ttacaggggt agcggcggta gtgcttttca gcaaatcgac tgcgtgctga cagttttgct
     1441 tgctggtgta gccttcgccc tgagcgatga tttcatggtt ggctgctttc aaacgccaac
     1501 ggtattcgcc ttttgcgtct ttatagattt caaaatacat aaggtttctc ctatgaatga
     1561 gtacacgttt tcttaccgct ttaacggcaa gtcctggtca ttgagcattt gggcggacaa
     1621 ccctgaagaa gccagggcga aatttcgggc tgcacgagaa aatgcgcact atgacggcga
     1681 agttgtagca aaggtttata catttgtaaa tatttcgtgg gttaagaaat tgtacaagcg
     1741 gacaaaatat ttaatgggta tcaaagaatg acctaccgtg aattagttga acgtcagttg
     1801 gctgtgcgcc atgccgattt ggaattgggc ttaagccgcg cacgcgagca agagccgttt
     1861 gtcattcatg tttccgatct gttggataag gcaggcattg agtacgcggt acgcatggat
     1921 aaggattttc agacgacgtt tcaccttgaa tatccaatta cgaactatga cacctttaaa
     1981 cgtgcggttt ggcaaacttt gggggcgtat tactgtgttt gtaatgatgg tgatggactg
     2041 gagattgcca gcaatcgccc tgacggttac gccgtccgta tcgtattcgg cgatgtgccg
     2101 gtttaaaggg gttttaaatg gactttgaat ttggtttcag aaccctgtgg ccgattgcga
     2161 cggcggcatt ttggttttgg gtcaacggca tttcaggccg cctgaaagag gcggacaagc
     2221 gtatcgacga ccttaaagag gagttgcacg cggtcaagct ctcttatcac accaaggcgg
     2281 acgccaaggc agacagcact aatattgcgg cggccttgga gcgaattgaa aacaagttag
     2341 aaaaagtaaa cgaaaaactg gacaggaaag cagacaaatc atgagcgacc cgattttgga
     2401 tgccttggcg cgtattgaaa acaagactga tcaaacgctg aaaaatcaga aggaaatgca
     2461 ggcggaaatt gcgcaaattc gccaagacac gaaacgcacg gccattacat tcggcgcact
     2521 gggcggcggc gtgattacgg tcggctggga attgcttaaa gcgaaaatgg gactgtaatt
     2581 atggctcacc cgcaagaaat ccgtgaaaag ttacgccggc tctatgtgag cggcgagcaa
     2641 actttggaaa cggcggcctt gatgtgcgaa atcccgcaga ccactgcgcg tgcgtggaaa
     2701 cgtgcggata aggaaaaagg cgacgactgg gataagatgc gcgccgctta cactttggcc
     2761 ggtggcggta ttgaggattt gagccgtgcg atgttggccg gttttatggt gcagtacaac
     2821 agcacgatga cgatgctgca ggattcgagt accgaagatt tgccaccatc cgaccgcgcc
     2881 aagctgttgg ccagcctggc cgatgcgttt acgaaaaccg tatcggccaa tgcgcgtgtg
     2941 atgccggaaa cgtcaaaact ggcgacggct ttggaattga ttgagttctt gatggcgttt
     3001 gtacaagaaa aacatcccaa acatttgcct gcctttgtgg aggtattgga gccgtttggg
     3061 gtggaagtgg agaagaagtt tggttagagg ccaacaaatt tttttaaaag agtaatgagg
     3121 gtggcggcag ggatagcatt aattgcattt tccgtaagcg tacttaatgc agtgtccttg
     3181 attttcccta attctttttt caaccagctt ttttctgaat cagaaatctc tgcttggtct
     3241 atttttgccg caattaaagc ctgaatagtg tcgctgtgta gtttgactgt gacaacacca
     3301 agaatggcgg ataggccgcc atcatcggta aggaagtcta tgcccttatg attaattttg
     3361 cagtcaaaat ttttatgaag tgaatctatc gaagtaattt caattaaacc agattcttct
     3421 aagtaataaa tattttttaa aaaatattgg aattcatctg attgtaaagt tgctaggtgt
     3481 tgactttgaa tttcacaacc aagagtcaaa gccaagccca atccttgttt atcaacaggg
     3541 atgttagaag taataggtaa agaactacta gggaaaagag agttatacac cttggttgct
     3601 tttaggcagt tcgggtaatt atcacttaaa actcgtaaga ttttttcctg aatacctcta
     3661 tttaaccagt tcataaatta ttcctcatga aaacaaaaga attcctcaaa tcccttgccg
     3721 aactggccgc cagtttgcgc caagtcatcg aagcggaagt ggacggcttc gatgcgtcgc
     3781 ccaaggctat tgctgcacgc cgtgccaagg tgtttgaccc ggtaggcggt tacgagtatt
     3841 tcgtgaatac ctacttcccc cattatatcc gctcgcctga gaaatccgaa ctgcatgcgt
     3901 ttttattcag ccgtctgccg gagattatcc gctcccccaa aggggaaaat gaggcggtgg
     3961 gtgcgccgcg tggagagggt aagtcgacga aggttactca gttgtttacg ctgtggtgta
     4021 ttgtgaccgg ccaaaaacat tatgctgtta ttgtgatgga cagtatcgac caggcatatc
     4081 cgatgctgga agccatcaag gcggaacttg aatttaaccc gcgcttgaaa accgactttc
     4141 cggaagtatg cgggcagggc cgtgtatggc aggccggtac gattgtgacg gccaatgacg
     4201 ttaaagtcca agtggccggt agcggtaaaa agctgcgcgg tttgcgtcac ggcccttacc
     4261 gtcctgactt aactgttttg gacgatattg agaatgacga gcaagtccgc aaccccgaac
     4321 agcgcgacaa gctcaatgcg tggctgacta agaccgtatt gcctctgggc ggtgtcggtc
     4381 agaaatacga tgtgatttat atcggcacga ttttgcatta cgacagcgta cttaaccgca
     4441 ctttgaataa cccgttttgg cacggtatta agtttaaggc gatgaaacgc tggcctgacc
     4501 gcatggattt gtgggacagg tgggaggaac ttttccgaaa cgacggcgag acggtggccg
     4561 aggcgtttta tcaggcaaac aaagacgaga tggagcgcgg cgcggtcact tcttgggcgg
     4621 cgcgtggcgt actcgcgctg atgaaaatcc gtgcgcgtga cggccatgcg acgtttgatt
     4681 cagaatatca aaacgatccg gtcagtggcg aagatgcgcc gtttgccaag tcgatgaagt
     4741 tttggaacga cctgccgtcc gatttggtgt atttcggtgc gctcgacccg tcactcggaa
     4801 aggccggggc gagccgtgac ccgtccgcga ttatcattgg cggttatcaa cgtgtaaccg
     4861 gcaaactgta tgtcgtggag gctcagatta aaaaacgtct ccctgatttg attattgagg
     4921 acgttattcg attgcaccgt caatatcgtt gcaaactgtg gtttgttgag actgttcaat
     4981 ttcaggaatt tctgaaagac gagctggtca agcgcagcgc ggcgcgtgga atacctgtcc
     5041 cagcgcgggc ggtcaaaccg gtatcggaca agctgttgcg gatcgagact ttacagcctc
     5101 acatggcgaa cggtttgatt ctgttgaatg agagccaaca aacgctgata cagcagttcc
     5161 gccattttcc aaaggctgat catgatgatg gtcctgatgc cgtgcatatg ctctggtcgg
     5221 gggcggtggc caattgtgtg ccgatagaat ggcaaagccc taccgataac gattttgatg
     5281 acgagataaa aagtaaatgg agccgataat ggcaaaaaag aacaataaaa ctaaaatcca
     5341 aaagcccgaa gctgcattgc agacggacgt ggctcaaatt acggcgaccg gtcgggttat
     5401 cgccgagcat ccgtccaatt ttattacgcc gcaaaagatg cgggccctct tcgaggacgc
     5461 agaaagcggc gacatccgcg cccaacacga gcttttcgcg gacattgagg agcgcgacag
     5521 cgacatcgcg gcaaatatgg ggacgcgcaa acgcgcgctg ctgacgctca actggcgcgt
     5581 cgccccgccg cgaaatgcga cgcccgaaga agaaaagctg tccgaccaag cctacgaaat
     5641 gatggacagc ctgcctaccc tcgaagacct gattatggat ttgatggacg cggtagggca
     5701 cggattttct gcgttggagg tcgagtgggt attttcagac ggcctttacc taccccgaaa
     5761 ctttatccac cgcccgcaaa gctggttcaa atgggacaaa gacaacgggc tgctgctgcg
     5821 tacccgcgaa aatccggaag gcgaagcgtt gtggccgctg ggctgggtcg ttcataccca
     5881 aaaatcgcgc agcgtccagc aggcgcgcaa cgggcttttc cgcacgcttt cctggctgta
     5941 tatgttcaaa cactacgccg tccacgattt tgccgagttt ttggagctgt acggcatgcc
     6001 catccgtatc ggcaaatacg gcgcgggcgc aaccaaagag gaaaaaaaca ccctgcttcg
     6061 agcggtggcg gaaatcggtc acaacgcggc aggcatcatg ccagaaggta tggaaataga
     6121 gctccacaac gcggcaaacg gtacgacggc aaccagcaat ccgtttttgc agatggccga
     6181 ctggtgcgaa aaatcggcgg cgcggctgat tttggggcaa acgctgacca gcggtgcgga
     6241 cggaaaatcc agcaccaacg cgctgggcaa tatccacaac gaggtacgcc gcgatttgct
     6301 ggtgtcggac gcaaaacagg tggcgcaaac catcacaagc caaatcatcg gaccgttcct
     6361 gcaaatcaac tatccccatg ccgacccaaa ccgcgtgccg aaatttgaat ttgacacgcg
     6421 cgagccgaaa gacatcgcgg tctttgccga cgctatcccg aaactggtgg atgtcggcgt
     6481 acaaatcccc gaaagctggg tgcgcgacaa actggtcatt ccagatgtgc aggagggtga
     6541 ggctgtgttg gtgcggcagg taccggacaa tccggtaaac agaactgcat tggcggcttt
     6601 atccgcccac accgtaccat ctaaggctac gggcaggcat caggaaatat tggacggcgc
     6661 gttggatgac gcgctggttg agcccgattt caattctcag ctcaacccga tggtgcgtca
     6721 ggcggttgcc gcacttaatg cttgcaacag ctacgaggag gcagatgccg cactgaatgc
     6781 gctttatccg aatttggaca acgcgaaact gcgtacctat atgcagcagg ccttgtttat
     6841 cagcgatatt ttgggacaag accatgcccg cgcctgattt gggatttgcc ttaagtctgc
     6901 cgccaaaaaa ggcaatcgag tggctggaaa gtaaaaaggt tacggcggag agctaccgca
     6961 atctgacagc ctccgaaatt gccaaagtct atacgattgc ccgcatgacc gacttggata
     7021 tgctcaacga catcaaaact tcgatggttg aatcggcaaa aagtggacag tcgtttgacg
     7081 attggcgaaa aggtatcttg aatctgctca gcaacaaggg ctggctgcat ccgaacgggc
     7141 ataacggtaa ggatatcatc gacccagcca ccggcgaggt attcggttcg ccgcggaggt
     7201 tggagacgat ttaccgtacc aatatgcaaa ctgcctacaa cgccggtcaa tatcaaggat
     7261 atatggcaaa tattgatgca cgaccttatt ggatgtatga cgcggtaggc gacagccgca
     7321 cccgtccggc gcattcggca atagacgggc tggtgtaccg ctacgacgac ccgttttggg
     7381 caacgtttta cccgcccaac ggctacaact gccgctgctc ggtcatcgcg ctgtcggagc
     7441 gggatgtgga acgccagggg cggattgttg ggcaaagcac ggcggacaat ctggtcgaga
     7501 cccataaaat ctacaacaaa aaaggcgata cttatctgac ccttgcctat aaagcaccgg
     7561 atggcagtct gtacacgacc gatcgaggat ttgattacaa cgccggacga atgaactacc
     7621 gccccgattt agacaagtac gaccgtgcgt tggcgcatca atttgccaaa gcggaaatgg
     7681 gtggtgcgga ttttaaaacc agctttaaac agcttgaaaa agagttttat gaagtcaagc
     7741 aacgtttgga tattgatggc aagcccgata aagagcagaa aatcaaaatc cgaaatgcgc
     7801 tatcaagaca gcttaaattt gctgcgggtg tattgagcaa ggaaacgcaa gaattggcag
     7861 gtatgacacg agcgacggtg tggctgtctg atgatacgtt ggttaaacag gtagacagcc
     7921 gtgaggggca gaatttcgat gactcctact atgctttttt gccggatatg ctgcaaaacc
     7981 ctgaacatgt catccgcgac aatcgtgaat tgattttcac agctcgctat aaaggctcgg
     8041 cattgtgggc agttttaaaa tatattaagg aggtggatga gatttatcta cagtcgtacc
     8101 gaatcagtaa cgacaaagag attgccaaat ttatggcgaa gaagaaagta ttgaaataga
     8161 cgttgggcaa ggctcgaaat cacttgcaca cgctctcgga cgccctaacg ggcaggctgc
     8221 ggtatcgagg ttatcaccgc ttttccaacg tctgtacgga ataataccat gattgatgtc
     8281 aaaatagaca atatctttgt cgtcctaaac caaatcgagc ggcttggcaa cgggatcgaa
     8341 aaccgctacc tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt caagctcaat
     8401 ttccgctacg caggccgtcc gaaatggttg ggctaaaata ccgcgacggc aagccgcttt
     8461 cggattcggg tcgtctgaaa gacagttttt ccacactgtc agacaacgat acagcccttg
     8521 tcggtacgaa tatcgtctat gccgccatcc acaacttcgg cggtatggcg gggcgcaacc
     8581 gcaaagttcg gattccgcaa cgggaatttt tgacgctgac ggacgacgac aaacaggctt
     8641 tgatggacga tgtgcaggat tatttttcgg gtctgatacc gtgaatttat aaaaccctca
     8701 aaaacgcgct ttttagcgcg tttttttatg cgggtaatac aaacccctgc ccaagatata
     8761 aaaatcaatc ctagacgctt ctaaaaagcc cctgaaaacg attaattgtg tatcgcgcgg
     8821 acaggtttta aaaaaatggc gggagggttt gaagcacgcc tactctttgt tgttttttca
     8881 aataggcaaa atgaccgtat tgagagaggt acacatgtcc aaaaatgcac aaaaaaccct
     8941 acttgccgtg tgcagtttcg aggtgcagcc aaaagacggg cgaatccaac tgctgccata
     9001 tggcgaattt cgcgcagtag acggtcgtcc gactgatgtc cctgcgtggt atctgaccga
     9061 agaaaacggt catgatgtcg cgttgttggc caacagctcg cgcaatcagt tggttgtcga
     9121 ttatgaacac cagacgctct acaaagagaa aaacggacaa cctgcacctg ccgccggttg
     9181 gatgcgttgg ctggagttca cgcctaaagg catgtttgcc gaagtggagt ggacggacaa
     9241 ggcggctgcg gcaattgccg caaaagagta tcgctacatc tctgctgtgt tttcctatga
     9301 cacaaaggga tatgtaagca aaatttttca cgccgcgctg acaaatttcc ccgcgttgga
     9361 cggtatggac gaggtgctgg cggcagcgtc ggcgcaaatt ttaaaaccgg aaacggagca
     9421 aaaccctatg aaagagttgt tacagcaact gttcgacctg cctgatgcgg gcgaagaaga
     9481 actgaaggcg gcattgtccg cgctcgtgga agccaagccg aaagacgtgg cattgtctgc
     9541 cgacgtgttc gcgcagctgg cggaaaaaga cagccgcatc gcggcattga cggcgcaaac
     9601 cgccaagcct gatttgacta aatacgcgcc tatctcagtg gttcaagagc tgcaaagcaa
     9661 agtcgccgcg ctgactgcca agcaggaagc agacaaaggc aacgaattga ttaccgccgc
     9721 gctgacttca ggcaaattgc tgcctgctca gaaggagtgg gcaaaaggcg tattgaaaca
     9781 gccgggcggc ttggcatttt tgaccggctt tattgaaaac gcccagccgg tcgctgcact
     9841 ggcaggctcg caaacgggcg gcaaagcacc cgacgaacgc gtcgccgcac tgactgcgga
     9901 agaggcagcc gcagcaaaaa tgctgggcat gtccggcgaa gaatttgtaa aaatcaaaga
     9961 aagcgaaggt aagtaatgga caaatcagcg attttgaccg caatcacggc agcattccgc
    10021 aaagaatttc aaacggcctt ggattcggat ttcaagtgca acactaaggt ac
//
LOCUS       DAWISC010000061         9861 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWISC010000061 DAWISC010000000
VERSION     DAWISC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.061"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..168)
                     /locus_tag="WIW87_001091"
     CDS             complement(<1..168)
                     /locus_tag="WIW87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174261.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYSGLNF"
     gene            complement(193..2250)
                     /gene="ppk1"
                     /locus_tag="WIW87_001092"
     CDS             complement(193..2250)
                     /gene="ppk1"
                     /locus_tag="WIW87_001092"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ5174262.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDQNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTDTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNILFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYYFHNNGTDDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIHEGLTMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2419..2862)
                     /locus_tag="WIW87_001093"
     CDS             complement(2419..2862)
                     /locus_tag="WIW87_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ5174263.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(2869..3384)
                     /locus_tag="WIW87_001094"
     CDS             complement(2869..3384)
                     /locus_tag="WIW87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ5174264.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDESMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3654..6284
                     /locus_tag="WIW87_001095"
     CDS             3654..6284
                     /locus_tag="WIW87_001095"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ5174265.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6418..7221
                     /locus_tag="WIW87_001096"
     CDS             6418..7221
                     /locus_tag="WIW87_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5174266.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDNS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKSINCAQLPRYFDFKS"
     gene            7341..7472
                     /locus_tag="WIW87_001097"
     CDS             7341..7472
                     /locus_tag="WIW87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ5174267.1"
                     /translation="MRDFDKPAEEAAEYAAEGAVKFIEGKPAKKIIAVPGRLENIVV"
     gene            7495..8424
                     /locus_tag="WIW87_001098"
     CDS             7495..8424
                     /locus_tag="WIW87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5174268.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFKRILCLSA
                     VISVLGACAVVADVYGHDSATMNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFR
                     RMLPYADAANNTSHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAI
                     MGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGL
                     TLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNN
                     ARIENLKRLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(8807..9118)
                     /locus_tag="WIW87_001099"
     CDS             complement(8807..9118)
                     /locus_tag="WIW87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ5174269.1"
                     /translation="MKYKNEALAAIHEMMEGAYNIGAIDKKTMRDFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(9115..9483)
                     /locus_tag="WIW87_001100"
     CDS             complement(9115..9483)
                     /locus_tag="WIW87_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ5174270.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADKAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
BASE COUNT         2319 a         2545 c         2671 g         2326 t
ORIGIN      
        1 aaaatttaat ccactataat gccgtctgga atgcggcggc ttggggttgg gagcgtatag
       61 gggaagtgct tgtgcaggaa tggcgaaggt gtgtttttga ctccggtccg ccctgtttag
      121 attgctgtaa aaccatgccg tctgaaacgg aaaggtttca gacggcatgg tttttgattg
      181 gggcggtgcg ggtcagcctc cgagcagtgt ccacaaatcg ttttgcaggt cggcttcgga
      241 ttcgccctcg gcaggtgcgg cgcggatata gccgccgtcg ggctgcatca gccacgcgtg
      301 ggtgttgtcg tccagtgcca tggtcagtcc ttcatgtata acgcgctttt tgagttcggg
      361 cgcggtaatc ggcgtggcgg tttcgatgcg gcggaagaag ttgcgcccca tccaatccgc
      421 gctggagata aaggtatcgt ccgtgccgtt gttatggaag taatacacgc gcgcgtgttc
      481 gagctgcctg ccgacgatgg agcggacgcg gatgttttcg gacaagcctt ttacacccgg
      541 gcgcaaggtg cacataccgc gcacaatcaa atcgatttgt acgcctgccg cgcttgcccg
      601 atacagggct tcgatgacgg tcggttcgat gagcgaattc atcttggcgg taatccgcgc
      661 cggtttgccg gcttttgcgt gttcggtttc gcgtgcgatg cggtcgataa ccattttgtg
      721 cagggtaaac ggactttggt agagtttgtt cagccgcccg ggtttgccca aacctgtgat
      781 ttccataaac aatatgttca catcggcggt gatttgttcg tcggcggtaa tgaggccgaa
      841 gtcggtgtag atgcgcgatg tgccttggtg gtagttgccc gtgccgagat gggcgtaacg
      901 tttgagcacg ccgtcttcgc ggcggatgac cagtgccatt ttggcgtgga ctttgtagcc
      961 gaacacgccg tacacgacgt gcgcgcccgc ctcttcgagc tgcttcgccc agttgacgtt
     1021 gttggcttcg tcaaaacgcg ccatcagttc gacgacgacg gttacttgtt tgccggcgag
     1081 tgccgccttc atcagggcgc ggacgagttc ggaacgcgtg ccggtgcggt aaatcgtcat
     1141 tttgacggca agcacggcgg ggtcggcggc ggcttcgcgc atcatttcga caacggggtc
     1201 gaacgattgg taggggtggt gcagcaggat gggcgattgg cgcaccaaat cgaatatgga
     1261 cgcggttttg cccaaggctt tcagtctgcc cggcgtgtgt gtgggaaatt tcaaatcggg
     1321 gcggttgact aggtcgggga cggcgttgag gcgcacgagg ttgaccgggc ctttgacctg
     1381 atagagttcg gcggcggtca gtttgaattg cgcgagcaga aagtcgcgga tgtaggcggg
     1441 acaggtgtcg gcgacttcga gccgcacgcc gtcgccgtat tcgcggtcgt gcaactcgtt
     1501 ttgaatggcg gcgcggaggt tttgcaggtc ttcttcgtca acggtcaagt cgctgtcgcg
     1561 cgtcaggcgg aactggtggc agcctttgac gttcatgccc gggaagagtt ttccgacgtg
     1621 ggcgtgcagg atggaggaga ggaagacgaa gccgtgtccg ccgccacaca gttcggacgg
     1681 caggggaaca acgcgcggca ggatgcgtgg tgcctgcaca atcgccatcc ccgaaggcct
     1741 gccgaacgcg tctgtgccgt cgagttcgac ggcgaagttg agcgatttgt tcagcgggcg
     1801 cgggaagggg tgggaagggt cgagtccgat gggggtcagg atcggcagca attcgcggtc
     1861 gaaatagtct tcaatccatt ttttctgtgt gtctgtccaa tttcggcggc ggtaaaaatg
     1921 gatgccttct tgtgccagct cgggctgaag gacgttgttg aacaggtcgt actggtgccg
     1981 tatcagggag cgcgccgctt cggtaacgtc ggcgatggtt tcagacggca ttttgccgtt
     2041 gtccagcctg cgctgcgggc agcgtttgtg ttcgcgcttc agccacgcca tacggacttc
     2101 gaaaaactcg tcgaggttgg atgaaacgat gcacaggaag cgcaggcgtt ccaaaagggg
     2161 gacgttttgg tcttccgcct gcgccaacac gcggcggttg aatgccagca agctcagttc
     2221 gcggcagagg atgcggtttt gttcgggcat aaggttctcc caaagatggt tgttgttcgg
     2281 tcggagcggg caggcaatgc cgtctgaagg cttggcggca tctgcgccgg tttcagacgg
     2341 catgacgtgg cggacagtct gtttcaggca tcggggcaat gtgaaaacag ataccggcgc
     2401 gtgcggtatc cgtctgtttc aaatcacttc caacacaaaa taatgacgca gtttttcgta
     2461 caccgcatcg ctgacgttga tgcagccgtt ggtcataatc ctgtcggaca cggacgggga
     2521 ggcgatgcgt tcgttccgcc tttccgacgg tatctgattc caaacgcggt gcagggcgaa
     2581 aagaaaatca ccctcctgct tgaaaccgat gacttcgccg ccgtatccgg gcttgtcggt
     2641 actgttcagc gtcagcccga acgtcccctt gggcgtggca acaccgatga ggacggggtg
     2701 gcactgacgg tcgtcagcga aacagagttc cgctttggat gtatcgacga tgactttttt
     2761 cttttggata aagtcgccga ccgtatccgg ctgcccctgt gcatatacgg gggcaaccat
     2821 tgacaggccg cataatatcc ctaaaaacag gcggaacggc atgtcggatt attggcggat
     2881 acgtttcggt tttgccggca cgatttcgcg gatgatgact tgcggctcgg ctttgggtgc
     2941 gggcggcgga caggcggcat ctttcgggaa gacggggttc cagtagaagc tgcgggcaaa
     3001 tttgtcttta tcgaaaatca ctttgtattg gcaggtagta acatcttcta cgccggaagt
     3061 gttttcaggg tcgataccta cgcccggggt atggaagtgg aacaggtaat cccattcgcg
     3121 cacgccgtac atactttcgt cgtaatgcgg gcggcccagg attttgtaga tgtcgtcttt
     3181 ggtcaggccg gggcgcatct gatccagttc gtcataagtc gggaatgtgc cgcgcttgtt
     3241 gtcgagcgtt acggaatagg gtttcgggaa aaccggattg tcggtcgtgc cgtcggcttt
     3301 gacgttgctt ttggttgcgc aggcagacag aacgcccgcc gccaatactg ccaagcccgg
     3361 tttgacgatt tgtttgattt tcatatgcag aatcctcttt tacccgatgc ccgtctgcct
     3421 tttgttcgga agagccgcat caggagagtt ttaaacattt gattttggtt tgtaatatta
     3481 gcataaaaaa cgtgcgtatc aataaacgcg gtgtatttgt acggcatacg gaataatgcg
     3541 cgtgcgaatt tacgcatcct gccggcaatc tgccgattcg ccgcttccgg caacctgtta
     3601 taattgatgc cgactttatt tttccaacgt ttttaagcga aaacagaatg actatgcaag
     3661 aacaataccg gcccgccgcc attgagcctg cggcgcagaa aaaatgggac gacgcccgta
     3721 ttttcaacgt ctccgaagac gcttccaaac ccaaatatta ttgcctctcc atgttcccct
     3781 atcccagcgg caagctgcac atggggcatg tgcgcaacta caccatcggc gatgtattga
     3841 gccgcttcaa acttttaaac ggcttcaacg tcatgcagcc tatgggttgg gacgcgttcg
     3901 gcatgccggc ggaaaacgcg gcgatgaaaa acaacgtcgc ccctgccgct tggacgtacg
     3961 ataacatcga atacatgaaa acccagctca aaagcctagg ttttgcgatt gactgggcgc
     4021 gcgaaaccgc aacctgcaaa cccgaatact accgctggga acaatggctg tttaccaagc
     4081 tgtttgaaaa aggcatcgtt tatcgcaaaa acggcacggt aaactgggac cctgtcgacc
     4141 aaaccgtcct tgccaacgag caagtcatcg acggacgcgg ctggcgttcc ggcgcattga
     4201 tcgaaaaacg cgaaatcccg atgtattact tcaaaatcac ggattacgcc gaagagctgc
     4261 tcaacgactt ggacaagctg gaacactggc cggaacaagt caaaaccatg cagcgcaact
     4321 ggatcggcaa atcgcgcggt atgaccgtgc gcttcgccgt ttcagacgac agcaaacaag
     4381 gcttggaagg cgattacgcg aaattcctgc aagtttatac cacccgcccc gacacgctga
     4441 tgggtgcgac ttatgttgcc gttgccgccg agcatccgct ggcagccgcc gcagccgccg
     4501 acaaacccga attgcaggca tttattgccg aatgcaaagc cggttcggtt gccgaagccg
     4561 atatggcgac gatggagaaa aaaggcgtgc cgaccggccg ctacgtcgtc aacccgctca
     4621 acggcgacaa gctggaagtg tggattgcca actatgtatt gtggggctac ggcgacggcg
     4681 cggtgatggc ggttccggcg cacgacgaac gcgattttga gttcgccacc aaatacaatc
     4741 tgccgaaaaa acaagtcatt gccgtcggcg acaacgcatt tgacgaaaac caatggcaag
     4801 aatggtacgg cgacaaagaa aacggcgtat tggtcaacag cggcgacttg gacggcttgg
     4861 attttcagac ggcattcgac gccgtcgccg ccaagctgca aagccaaggc gcgggcgaac
     4921 cgaaaaccca ataccgcctg cgcgactggg gcatttcgcg ccaacgctac tggggctgcc
     4981 cgattcccat cgtccattgc gaacaatgcg gcgacgtacc cgtccctgcc gaccaattgc
     5041 cggtcgtttt gcctgaaaac gtcgtacccg acggcatggg ttcgccgctg gcaaaaatgc
     5101 ccgagtttta cgaaactgcc tgcccatgct gcggcggcgc ggcgaaacgc gaaaccgaca
     5161 ccatggacac cttcatggaa tcgagctggt atttcttccg ctacatgtcg ccaaaatttt
     5221 cagacggcat ggtcgatcct gccgccgcga aatactgggg cgcggtcgac caatacatcg
     5281 gcggcatcga acacgcgatt ttgcacctct tgtacgcgcg tttcttcacc aaactgatgc
     5341 gcgacgaagg tctagtcaat gttgacgaac cgtttgaacg cctgctgacg caaggcatgg
     5401 tcgtttgcga aacctactac cgcgaaaacg acaaaggcgg caaagattgg atcaaccccg
     5461 ccgatgtcga gctgacgttc gacgacaaag gccgccccat ttccgccgtc ctcaaagccg
     5521 acgggctgcc cgtcgtcatc agcggcacgg aaaaaatgtc caagtccaaa aacaacggcg
     5581 tcgatccgca agaactgatt aacgcctacg gcgcggacac cgcccgcctg tttatgatgt
     5641 tcgccgcacc gcccgaacag tccctcgaat ggagcgacag cggcgtcgaa ggcgcacacc
     5701 gcttcctgcg ccgcctgtgg cgtaccgttt acgaatactt gaagcaaggc gaagcggtca
     5761 aagcgttcgc aggcagccaa gacggtttgt ctaaagaact caaagacctg cgccacaaac
     5821 tgcacgcgac cactgccaaa gtcagcgacg actacggccg ccgccagcag ttcaacaccg
     5881 ccatcgccgc cgtgatggaa ttgctcaacc aatacgacaa aaccgacacc ggcggcgaac
     5941 aaggacgcgc cgtcgcccaa gaagtattgg aaaccgccgt acgcctgttg tggcccatcg
     6001 tgccgcacat ctgcgaaacc ctgtggagcg aattgaacgg cgcgaaactg tgggaagcag
     6061 gctggccgac agtcgacgaa gccgccttgg tcaaatccga aatcgaagtg atggttcaag
     6121 tcaacggcaa actgcgcggc aaaatcactg ttgccgccga cgcatccaaa gccgacctcg
     6181 aagccgccgc actcgccacc gaaggcgcgg tgaaattcat ggaaggcaaa ccggcgaaga
     6241 aaatcatcgt cgtaccgggc agactggtga acatcgtcgt ctaaccgctt ttaaggttta
     6301 gccacacgga taaaggccgt ctgaaacttg gaaacagggt ttcagacggc ctttttaagg
     6361 tggattgtgt tgtctgcatg acgaaccccg aataactgaa atctcaaaat ccaaaacatg
     6421 aatttatttt tcgataccga attgggaaag caacaaaata aagcaaccca taaaatccgt
     6481 gtaatgagcg aggcttggtt ggaaaaaaac ggctactgcc cctgttgcgg aagcaagccg
     6541 atgcagagat ttgccaataa caaacctgtt gcagacctct tttgcccaaa ttgccacgag
     6601 caatatgaat taaagagtaa aaatcaaaaa accataggca acagcgtgcc tgacggtgca
     6661 tatcacacca tgttggagcg catccggtca gataccaacc ccaacttttt ctttcttgca
     6721 tataaaaaag cggattactc catacggcaa ttggtgcttg tacccaaaca tttcatcacg
     6781 ccggatatga ttattcccag aaataaaggc attaaaaacc ggccgaacca cattatgtgt
     6841 tctatcaatc tcgccccttt gcctgaaagc ggcaaaatat tcttaataga caattcccgc
     6901 attatcgaac ccgaaaccgt tctgaaaaaa tggcaatcca atttgttttt acgcaaccaa
     6961 aatgcggagc gcaaaggctg gcttctggct gttatgaaat gtatcgacca actccccgaa
     7021 gaattcacat tgtcgcaaat gtatgaattt gaaaacaaac tatccatcca atttccccaa
     7081 aacaaccata tcagagacaa aatccgccaa cagttgcaaa ttctgcgcga ccaaaatacg
     7141 atcgaattca ttggtcgcgg actttacaaa aaatcaataa attgcgccca actcccaagg
     7201 tattttgatt tcaaatcatg atactgaatt cacaagggat atttattctt ctgccatttt
     7261 gaaaacagtt gaagttcagc gcaatattgc cgcaaaaggc agcagcagcc gtaaaaaagt
     7321 tggagaatta ttggcaattt atgcgtgatt tcgacaaacc ggctgaagaa gctgccgaat
     7381 acgccgctga aggcgcggtg aaattcatag aaggcaagcc tgccaagaaa atcatcgccg
     7441 taccgggcag actggagaac atcgtggtgt aaatcttttt aaaaacatta aacaatgtta
     7501 agatgccgtc cgaaatccgt tttggattca gacggcattt ttttgaaatt taatttttta
     7561 aggagtaaac ctaaatatga aatttccttc cttccttcct ttaaacggat actctgcctg
     7621 tcggcagtaa tctcggtatt gggggcttgt gcggtcgttg ctgatgttta cggtcatgat
     7681 tccgccacaa tgaacgctgc ggctgccaaa gattatatga aaacggttga gttaaacaag
     7741 tctgccggca atgtcgatac cacatccaga acagcccgca gggtgcaggc agtatttcga
     7801 cgtatgctgc cttatgccga tgcggcaaat aataccagcc ataagtttga ctggaaaatg
     7861 acggttttca aaaacgatga gctgaacgcg tgggcaatgc ccggtggaaa aatggcgttt
     7921 tatacgggga tagtcgacaa actcaagctg accgatgacg aaattgccgc cattatgggg
     7981 catgaaatga cgcacgccct gcatgaacac ggtaaaaata aggtcgggca gcaaatcttg
     8041 accaatacgg cggcgcagat aggcacgcag attatattag acaaaaaacc ggatactaat
     8101 ccggaattgg tcggattggg tatggatatt ttggggacgt acggtcttac cttgccttat
     8161 agccgcagct tggaagaaga agccgatgag gggggaatga tgttgatggc gcaggcaggc
     8221 tatcatccgg cggccgctgt cagggtttgg gaaaaaatga atcaggaaaa cgaccaaaac
     8281 ggctttattt atgctattac ctctactcat ccgacaaaca atgcccgtat agaaaatcta
     8341 aaacggttgt tgccgaccgt tatgccggtt tatgagcaaa gtgtcagaaa taaggggcgc
     8401 gttaataaaa aacgtcggcg ttaagggaag gggatcgaga atttgagcca ttgtttcaaa
     8461 atgccgtctg aaatcgtttg agattcagac tgcatttaaa cagttctgtg ccccacccgt
     8521 tttgccttca agcccgcgcc gcttaaatcc aatccccccc tgcaacgggc gcaaaaaatc
     8581 cccgtacctc gcggtcggtg atgtttcggg tttcgcgccc gtctgcgccg tgccttgctg
     8641 tcgggctatg tcgtctgcac cgccgccgtt tcggcttggt ttgatgcggt cgggcattta
     8701 tgcggctacg ccctgatatt cagttcggcg gtcttgcctg tcttggcagg catattcagc
     8761 agcgcgtcta ttgtgaactt gctgattttg ccgtttgcca agtcggctac gcaatggctt
     8821 cgatgccctt gtttttgacg atggtcagca gcttcaacgc cgcgccgctc ggctttttaa
     8881 cgccccgctc ccaagccgaa acgtgatttt ttcccacgtt gagatagatg gcgaaagcgg
     8941 cttgcgatag tgcctccttc tccctgattg ccttgatgtc tccgccgctc aacggtttga
     9001 tttcggtcag gcaggacttg tcaaagtcgc gcatggtctt tttgtcgatt gcgccgatgt
     9061 tgtaagcccc ttccatcatt tcatgaatgg cggctaatgc ctcgtttttg tatttcatga
     9121 ttctatctcc gtgataatgc cgtcttcttt caaagccgcc agctctgccc gcgtgtattt
     9181 cagataatat gcggcggctt ttcggtaaac gtcaagttct ttatccgaaa tgttttccct
     9241 gtcgttcttg gcaaaggcgt aaacaaaaaa tgccttgtct gcctgtttga acagtatcag
     9301 actgcgataa ccgccgcttc tgccttgtcc ttgccttgct atgcgctgct taatcacacc
     9361 gccgcctaaa tctgcgtcta tcagcccgtt atccgcccgc tctaccgctt ccagcagctc
     9421 ggaatcgttg attttgtgct tcttggcaaa tttcactatc cattggtttt tgaatatccg
     9481 cattgtgtag ccgttgatta gtgatacttg gaattatacc agagacagac aacacggcaa
     9541 gctgcctttt ttatgccccg caatttctcc gtttatgccg cctgaaacac tggcggctaa
     9601 cctgattatt tttttaatca atcgctttta atggctcaaa ttgcgttttt agcgcgtttc
     9661 gctgtcttcc tattccccgt atataaccaa gcggtttaac cgccattcct gctcaaaata
     9721 ggcgcggttc cgtgcgtttt ggcttttcat tccgctacgc tcccactgcc aagacggtga
     9781 aaaaccggaa aaccccaaaa gttgttaccg attcgttacc atttgttacc gcatttgtta
     9841 ccaatttttt atctttttct t
//
LOCUS       DAWISC010000062         9845 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWISC010000062 DAWISC010000000
VERSION     DAWISC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.062"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(62..676)
                     /locus_tag="WIW87_001101"
     CDS             complement(62..676)
                     /locus_tag="WIW87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ5174271.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WIW87_001102"
     CDS             complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WIW87_001102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ5174272.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYRLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTSEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2966..3190
                     /locus_tag="WIW87_001103"
     CDS             2966..3190
                     /locus_tag="WIW87_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174273.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            complement(3258..4559)
                     /locus_tag="WIW87_001104"
     CDS             complement(3258..4559)
                     /locus_tag="WIW87_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ5174274.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPI
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRTV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAYADFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPQPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(4549..5460)
                     /locus_tag="WIW87_001105"
     CDS             complement(4549..5460)
                     /locus_tag="WIW87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5174275.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5568..5966
                     /locus_tag="WIW87_001106"
     CDS             5568..5966
                     /locus_tag="WIW87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ5174276.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            6015..6620
                     /locus_tag="WIW87_001107"
     CDS             6015..6620
                     /locus_tag="WIW87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ5174277.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVVGGIEGAAGEKTFEPVAERLKVFGA"
     gene            6806..8916
                     /locus_tag="WIW87_001108"
                     /pseudo
     CDS             6806..8916
                     /locus_tag="WIW87_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            9031..9726
                     /locus_tag="WIW87_001109"
     CDS             9031..9726
                     /locus_tag="WIW87_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5174278.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         2128 a         2525 c         2893 g         2299 t
ORIGIN      
        1 cgttttgagg ccggggtcgg aataaccgtt ttttgatgat tttccctccc cggctgtgtc
       61 atcaaaaccc caattgcctt tccaaactct ccaccagatt gtcatccagt ttcaaagcct
      121 gcgacaggcg ggcgaggaag acggtttctt tccgcgacaa atcggcacag accaaccttg
      181 ccgccagata ggcctccgcc gccaacgcct catcgttgcc gacggcggcg gcgatgtctt
      241 cgatgcttgc gggaaggcgg tattcggcgg cgagccatgc ggcagtttcg gggtctgtgc
      301 cgctttcctg ttcgatagtc cggcgttcgg cttcgtctat catgccgtct gaagcggcgg
      361 cggctatcat ggtgcgcaat acggtacggc tgtatgtttc ttcagtttct ccggcaggtt
      421 ggaaatcgct ttgtgttacg gttgcccgcc ctttgttttg ctgccacatc tgatagcccc
      481 ggtaggcgag gtagcccaaa gcggcggtcg aaccgatttt ggtgatggtt ttgcggtttt
      541 taccgttcag cagcatggag gcgacaccgg caaccagcgc gcctccgccg aatgaattga
      601 gcgggctgtc ggagaatgtg ttgccttttt tttgaaccgt gcttaagact tggttgagca
      661 gtcgggtaaa gttcatgaat ttttcctttc tgttgcggga agggcggtat gtttacccta
      721 tccttttaaa cggcggcagg ccggccaata attgttggcc gtaccgctgt gttttgatgc
      781 ggttgtcgag gatggttacg cggccgtagt cttgttcggt gcggatgagg cggccgacgg
      841 cctggatgag tttgatgccg gcttcgggga cggtgatttc gatgaagggg ttgccgccgc
      901 gctgttctat ccagcggttt tgggtttttt cgatggggtt gtcgggcatg gcgaagggaa
      961 gtttggcgat gatgacttgc acgcaggcgg tgccgggcag gtcgagtcct tcggcaaagc
     1021 tgtcgagtcc gaagatgatg ctggctttgc cttcttctat ggcccggtgg tgtttttgca
     1081 ggaggacggc tttgggtaat tcgccttgta cgagcaagag cggcaggtag tctccgggca
     1141 ggcgcagggc gacatcctgc atttgtttgc gcgaggaaaa caagacgagc gtgccgatgg
     1201 cttcggtggg cgaaataagc ttgggcagcc attcgatgac ggcggcggtg tgggcttcgg
     1261 ggtctttggg gctggcgtat atggggggga tgtagagttc gccctgtttt tcaaagtcaa
     1321 aggggctttt gagggcgagg gtggtggttt cgggcagcca ttgcagcccg gtttggcgca
     1381 gcatcaggtt gaagttgccc aaggattgca gggtggcgga agtcaatacc gcgcctgccg
     1441 cacgccgcca caggctgttg gcaaggtggg atgcgctgct gatggggctg gcgttgaaaa
     1501 tgtagtcgtt tttgtcgtcg gcgcggcggg ttatccattt cgccaacggt tcttcaccct
     1561 cgagggggac agtggagagc aaatcccaaa ccgcgctgat ttgttcgata cgggcgataa
     1621 aaagaccgaa ctcgctggtc aggcggtcga ggagcgcgcc gtcctgttct ttttcgcggc
     1681 gtgcggcaga aagcgcatcg ttcagcccga taacgtgttt gagcaggctg cgcgcagcaa
     1741 tggccgtatt ggaaacggtg gtttcgaggc cttcggggat tttgccgtct tcccacagcc
     1801 aagtcggttc gctgttggtt cgtctgtcgt tttcagacac ccccagactt aaagacggct
     1861 cttccgccaa atggaattgc cattcatgca ggctgtcgag caaggatgcg gcggcttcgt
     1921 cggctaggtt ggcaagttcg gctttatcgg tcagcgcggc aattttgccg gtcagctgcg
     1981 gcagtttttc cagcgtccaa acggcaatat tccatgaatg ttcggcggca aaacggctga
     2041 gggctttttt gggcaggtgg tgcgcttcgt cgatgcaata gaaactgttt tcgggcgcag
     2101 gcagaatcac gccgccgccc atactgatgt cggcaagcag aagatcgtgg ttggcaacga
     2161 cgacatcgac ggtttccaag acatcgcgtg ctaggtaaaa cggacattcc ggacggttgg
     2221 gacaggcggt tttcaggcag ccgtggcggt cgttggtcac tttgagccaa atcgcgtcat
     2281 cgattttttc cggccaagtg tcgcggtcgc cgttgaaccg tcgggcggaa aattcgtcgg
     2341 cgatgtcgcg cagcagcttc aattcttcgg gcttgggttt gctgtcccac aagacggcgg
     2401 gggcttcaaa gccgagcagg ttttgctggg cattgctttg cgtcagtcga tagagtttgt
     2461 aggggcagag atagcggccg cgccctttgg caagtgcgaa ggtcagttcc aaaccgcttt
     2521 tttcgaccag aaacggcagg tcgcggtcta ccaactgctc ctgcaaggca accgtcgcgc
     2581 tgctcacaat cagccgcttg ccgcgtgttt gcgccatgat gccgccggcc aaaaggtagg
     2641 ccaacgattt gcccacgccg gtcggccctt cgatcacggc aatgctctcg ccttcgcgct
     2701 tgggcggctc gccgccttct tcgcgcgcca acgtccgcga aaaagcgttg gcaaccgccg
     2761 caatcatttc ccgctgcgaa gcacgcggac ggaaaccggg caggtttttg ccgatgtttt
     2821 ggtaatggtc gcggatggcg tttttttcta aatcggtgag catggcgttt tgtacggcgg
     2881 tagaagtggg cttattttaa cattgcacgg aagcggtaca atatcgttgt cggaatgggg
     2941 ggtgaggtga atcgtgcgga cgtggttggt tttttggttg cagcgtttga aatacccgtt
     3001 gttgctttgg attgcggata tgttgctgta ccggttgttg ggcggcgcgg aaatcgaatg
     3061 cggccgttgc cctgtgccgc cgatgacgga ttggcagcat tttttgccgg cgatgggaac
     3121 ggtgtcggct tgggtggcgg tgatttgggc atacctgatg attgaaagtg aaaaaaacgg
     3181 aagatattga gtcattcgga cgcaatgccg tctgaaacgg aagttcagac ggcatttgtt
     3241 ttaggttgcc gtaccgctta gggaataccg gcgacaggat gggcgggata gccgtgggta
     3301 tcgaccgaac aggcaaaccg ccaaggcgtg tggacggtgt cggcggacag gtgggcaagc
     3361 tcgggaatgt gccgtctgac aaaggtgccg tcggggtcgg ttttgtgtgc ggcggcggca
     3421 atgtcggggc aggtgtgccg tgaggcggca agccgccagt tgccttggtt gattgctgca
     3481 tcgaaatcgg tcagctgtcg ggcaaaccat atctcgcctt cgcggcgggg gaggtttaaa
     3541 acgtggcaga aaaaatccgc gctcaagcgt ctcagggcgg ggtggaggct gccggttttg
     3601 tgcaaacagc gcatcgcggc atcgataatc ggaatgccgg tccggccctg ctgccaaagc
     3661 gtcaggcgca gggtgtgttc aggattgccg tctgaagggt cgtcatccgt gtgctgcaag
     3721 gcaagttgaa ggaaaaaatc gcggcggatg atgttgtccg cccacgcgtt cagacggcgt
     3781 tcgaggcttt cccgcgcgag caggcgcggc gagatgcagc cggcactcaa atacgcgccc
     3841 atcagcgaag tgtgtttgcg cgaggggaaa tcctttaaaa cggagtagga atccgcctgt
     3901 tcgagaaacc gccgccactg ccgccaagcc gccgtttcgc cgctgttttg cggcaggaag
     3961 atgccgtctg aaagcgcggc aggctgcggg gcggaaaggt tttcggggaa gggttggcgg
     4021 tatgccgcga ataggtccgg accggcgggg ggctgcttgg aaaagcggtc gagccatact
     4081 tcgcggtagc ggtcgaaatc ggcatatgcc gtgccgccgt cgggtatcag gtcggttttg
     4141 ccgaaaacgg cgcggtcgtt gacgaaggtt aacgcgatgc cgtgtttgtc caattcgtgc
     4201 caaagggcgt tgtcggcgag tttgtcggca aaagtatggg attcgtcggc gatgacggtg
     4261 cggatattga ggcggacggc gagccggacg agctcggcag gagatgccgc cgtgtagagc
     4321 gggatgccgc gccctgcaag cccttgggcg agttcggcgg cggattggcg gtagaacgcg
     4381 gcgcggcgag ggttgtctgt ttcggcatcg tcaatccaaa tgccgataat gggcaaacct
     4441 tcggcaacgg cggcgcataa ggcggcgttg tcgcggatgc ggaggttttg gcggaaccag
     4501 acgagcgtgt gtgcggcgca cgtgtccgca taaagggggc gggcggtttc agacggcatt
     4561 tcggcagcct ttcctgctgg cgattttttc gttcagaaaa tcgatgaagc tgcggacttt
     4621 cgcgcttaag aatgccctgt ctgcataaac ggcattcagc cggtcggtcg ggacggcgta
     4681 tccgggcagc agcctcacca gcgtgccgca gcgcaaatcg tgttccgccg cccaaagcgg
     4741 ctgataaccg atgcacgcgc ccgccttaat catttcgcgc atcatcagcg tgttgtcggt
     4801 acggatgacg ggggtcagtt caagccggta ttttttgccg tccgatttgc gggtgaggtc
     4861 gagtttctgc tggttggtgt aggtcggcag gacggcgggc agccccgcca cttcttccgg
     4921 cgtttccggc acgccgttgc gcctcaggaa atcgggcgag gcgagcaggg caaattcgat
     4981 ttccgccagt gggcgcgcaa tcagcgacgg ggacagggtt tgggaaacgc gcaacgccaa
     5041 atccacgcct tcggcaatca aatcgacgtg gcggttgtcc aaaatcagtt ctaatgccac
     5101 ttcgggataa cgttcgcggt attccgccag ccagttgcat atctggctgc cggcaaacca
     5161 cagcggcatc gttacgcgca gcagcccctg cggtttttcc gtccccccgg cggctttttg
     5221 cgcggcatcg tcgagcgtgt cgagcgcgta actgcattgc cggtagtatt cttccccggc
     5281 ttcggtcagg ctgaggttgc ggctgttgcg gtgcaggagt ttggcttgga cggtgttttc
     5341 caagtggctg acgtgtttgc ttgccattgc ggtggagatg ccgagcgcgt cggcggcgcg
     5401 ggtgaagccg ccgctttgga cgacttggcg gaaaaccttg aggctgaaca gggtgtccat
     5461 attttcttgt gtggaaaagt tgtatcaata aaagcagtat atatttgaaa aggggaaaca
     5521 tctatactct accgcctgaa atgaagacaa atatcaaagg agcttttatg tccgattgct
     5581 gcaaccgtat ccaaccggtt ttgctttctg ttttgcgtat cgtaaccgcc tacctgtttt
     5641 tgttgcacgg tacgtcgaaa atcttcgcct tccccattga aatgggcagc ggttcgcccg
     5701 gcgggctgtt gctgcttgcc ggtattttag aaattgtcgg cggcattttg ctggtgttgg
     5761 gcctgtttgc gcgccctgcc gcgtttgttt tgtccggcca gatggcggtt gcctatttta
     5821 tggcgcacgc ttccggaaat gctttgttcc cgattgccaa cggcggcgag tccgcagtgc
     5881 tgttctgctt cgtattcctc tatatcgcgg cggcgggcgg cggagcatgg tcgctggaca
     5941 ggctgttttt caagcgtaaa gcctgaatcg gactgcctaa agtgtatttt gttgaatgtt
     6001 tttgaggaaa agaaatgacc cgtcaatctc tgcaacaggc tgccgaaagc cgccgttcca
     6061 tttattcgtt aaataaaaat ctgcccgtcg gcaaagatga agttgtccaa atcgtcgaac
     6121 acgccgtttt gcacacacct tcttcgttca attcccaatc tgcccgcgtg gtcgtgctgt
     6181 ttggcgaaga gcatgataag gtgtggcaat ttgtcgaaga cgcgctgcgt gccgtcgtgc
     6241 ctgccgacag ttttgaaccg accgcgcaaa aattgaacct gtttaaggcg ggtgcggcaa
     6301 ccattttgtt ttatgaagat caaaatgtcg tcaaaggttt gcaggagcag ttccctgctt
     6361 atgccgctaa cttccccgtt tgggcggatc aggcaaacgc gatggtgcag tatgccgttt
     6421 ggacgacact tgccgcggtc ggcgtaggtg caaacctgca acattacaat cccttgcccg
     6481 atgcggcgat tgccaaagcg tggaatatcc ccgaaaactg gttgttgcgc gcgcaaatgg
     6541 ttgtcggcgg tattgaaggg gcggcaggtg aaaagacctt tgaaccagtt gcagaacgtt
     6601 tgaaagtgtt cggcgcataa tttcgcggtc aaaaaaatgc cgtctgaacc ctgttcagac
     6661 ggcatttttc agtatcaggc ggcgagtttt ccgcattctg agacctttgt ttacaaatat
     6721 catgttcaat atagttaaaa gaaattattc tcatttcctc cgtgaggcaa tataattcgg
     6781 ttgttttgtt aaattgagta taaaaatgaa aatatcattt catttagctt tattacccac
     6841 gctgattatt gcttccttcc ctgttgctgc cgccgatacg caggacaatg gtgaacatta
     6901 caccgccact ctgcccaccg tttccgtggt cggacagtcc gacaccagcg tactcaaagg
     6961 ctacatcaac tacgacgaag ccgccgttac ccgcaacgga cagctcatca aagaaacgcc
     7021 gcaaaccatc gatacgctca atatccagaa aaacaaaaat tacggtacga acgatttgag
     7081 ttccatcctc gaaggcaatg ccggcatcga cgctgcctac gatatgcgcg gcgaaagcat
     7141 tttcctgcgc ggttttcaag ccgacgcatc cgatatttac cgcgacggcg tgcgcgaaag
     7201 cggacaagtg cgccgcagta ctgccaacat cgagcgcgtg gaaattctga aaggcccgtc
     7261 ttccgtgctt tacggccgca ccaacggcgg tggcgtcatc aacatggtca gcaaatacgc
     7321 caacttcaaa caaagccgca acatcggagc ggtttacggc tcaagggcaa accgcagcct
     7381 gaatatggac attaacgaag tgctgaacaa aaacgtcgcc atccgtctca ccggcgaagt
     7441 cgggcgcgcc aattcgttcc gcagcggcat agacagcaaa aatgtcatgg tttcgcccag
     7501 cattaccgtc aaactcgaca acggcttgaa gtggacgggg caatacacct acgacaatgt
     7561 ggagcgcacg cccgaccgca gtccgaccaa gtccgtgtac gaccgcttcg gactgcctta
     7621 ccgcatgggg ttcgcccacc ggaacgattt tgtcaaagac aagctgcaag tttggcgttc
     7681 cgaccttgaa tacgccttca acgacaaatg gcgtgcccaa tggcagctcg cccaccgcac
     7741 ggcggcgcag gattttgatc atttctatgc aggcagcgaa aatggcaact taatcaaacg
     7801 taactacgcc tggcagcaga ccgacaacaa aaccctgtcg tccaatttca cgctcaacgg
     7861 cgactacacc atcggccgtt ttgaaaacca cttgaccgta ggcatggatt acagccgcga
     7921 acaccgcaac ccgaccttag gttacagccg cgcctttact gcttccatcg atccatacga
     7981 ccgagcaagc tggccggctt cgggcagatt gcagcctatc ctcacccaaa accgccacaa
     8041 agccgactcc tacggcatct ttgtgcaaaa catcttctcc gccacgcccg atttgaaatt
     8101 cgtcctcggc ggccgttacg acaaatacac ctttaattcc gaaaacaaac tcaccggcag
     8161 cagccgccag tacagcggcc actcgttcag ccccaacatc ggcgcagtgt ggaacatcaa
     8221 ccccgttcac acactttacg cctcgtataa caaaggtttc gcgccttatg gcggacgcgg
     8281 cggctatttg agcatcgata cgtcatcttc tgccgtgttt aacgccgacc ccgagtacac
     8341 ccgccaatac gaaaccggcg tcaaaagcag ttggctggac aatcgtttgg acaccacatt
     8401 gtccgcctac caaatcgaac gcttcaatat ccgctaccgc cccgacgcgg aaaataatcc
     8461 ctacacttgg gcagtcggcg gcaaacaccg ttcgcgtggc gtggaattgt ccgccatcgg
     8521 gcaaatcatc cccaaaaaac tctatctgcg cggttcgttg ggcgtgatgc aggcgaaagt
     8581 cgttgaagac aaaaaaaatc ccgaccgagt gggcatccat ttgaataata ccagcaacgt
     8641 taccggcaac ctgtttttcc gttatacccg accgaaaacc tctacggcga aatcggcgta
     8701 accggtacag gcaaacgcta cggttacaac tcaagaaata aagaagtgac tacgcttcca
     8761 ggctttgccc gagttgatgc catgcttggc tggaaccata aaaatgttaa cgttaccttt
     8821 gccgcagcca atctgttcaa tcaaaaatat tggcgttcgg actctatgcc gggtaatccg
     8881 cgcggctata ctgcccgggt aaattaccgt ttctgatgaa atcaggcaaa ggctgaaata
     8941 aaactaaaca cattttttca ctcaaatcga acacgccttc aataaaatgc cataaaatcc
     9001 gcacattaat ctgacacaca agagatacct atgaaactga aaaccttagc tttgacttca
     9061 ttgaccctgt tggcattggc cgcttgtagc aaacaggctg aaaccagtgt tccggcagac
     9121 agcgcccaaa gcagctcatc tgctccggca gcccctgctg agttgaacga aggtgtgaac
     9181 tacactgtat tgtctacgcc tattccgcaa cagcaggccg gtaaaatcga agtattggaa
     9241 tttttcggct acttctgccc gcattgcgcc catcttgagc cggtcttgag cgagcacatc
     9301 aaaacgttta aagacgatac ctatatgcgc cgggagcatg tcgtgtgggg tgatgaaatg
     9361 aaacctttgg cacgtttggc ggccgcagtg gaaatggccg gtgaatcaga taaagccaac
     9421 agccatattt tcgatgcgat ggttaatcaa aaaatcaatc tggccgatac cgataccctg
     9481 aaaaaatggc tgtccgagca aacagcgttt gacggcaaaa aagtattggc tgcatttgag
     9541 gctcctgaaa gccaagcgcg tgcggctcaa atggaagagt tgaccaataa attccaaatc
     9601 agcggcacac cgactgtgat tgtcggcggc aaataccaag ttgaatttaa agactggcag
     9661 tccggtatga ccacgattga ccagttggtg gataaagtac gcgaagagca gaaaaagccg
     9721 caataagttg aggattgaat gagtaaaggc catctgaaaa taggatttca gacggccttt
     9781 tgtatttagg ctttatagaa gagatgattg cttaaagcct tatggtttta aatcagaata
     9841 tatag
//
LOCUS       DAWISC010000063         9794 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWISC010000063 DAWISC010000000
VERSION     DAWISC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.063"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(10..1305)
                     /locus_tag="WIW87_001110"
     CDS             complement(10..1305)
                     /locus_tag="WIW87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ5174279.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLGG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLTYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WIW87_001111"
     CDS             complement(1431..2318)
                     /locus_tag="WIW87_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ5174280.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WIW87_001112"
     CDS             complement(2318..3283)
                     /locus_tag="WIW87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ5174281.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIW87_001113"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIW87_001113"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174282.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WIW87_001114"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WIW87_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIW87_001115"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIW87_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ5174283.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIW87_001116"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIW87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ5174284.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIW87_001117"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIW87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ5174285.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPEILLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIW87_001118"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIW87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ5174286.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8694..>9794
                     /locus_tag="WIW87_001119"
     CDS             8694..>9794
                     /locus_tag="WIW87_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ5174287.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIISVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2511 a         2612 c         2424 g         2247 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgcata aaaaacattc ggggctaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgctcagg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc cggaatatcg ggttcaaacc tgcataagta ttgacagcat agaccacatt
      601 tttgcactcg acgctgcctt cgggcgtgta aaccagccaa ccgttttgat gcggttcgat
      661 gcacgtcatc ggggattgct cgaaaatctg cgcaccggct tcggcagcgg cacgagcgat
      721 gcccaaagtg taagtgagcg gatgcaggtg tccggataag gggtcgaatt gtgccccttg
      781 gtacatatcg ctgtcaagct gctgtttcaa ctcggcttta tcccaaagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccactgctgc aactcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcacaa tcgacggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcctgctc
     1021 caagccgacc tgtttttcaa tttcccccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccaccc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 aggtgtattt tcggacggaa tgatacaggc tgtcgggcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ctggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggtcgcca agacattcat ctgaggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cgggcgcgcg cccaaatcca ttgcggcttc ttcgagcgac
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc atgccagcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca agatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatgc cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacagacaaa
     2941 ttaccgtcgt agtcagcgca gtcttaattg aagaccaata agtcatcaga tagatattgc
     3001 tgttttcacc gtcgccgaac ggattgagcg tactccaaaa attttggaag atgtctgcat
     3061 aattttggta gctgacagca atattcagac gacccaaatc ctcatctatc gtcgttaaag
     3121 gagtaaacgg cgggatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc gcacgctgcc
     3241 ccggacggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 ttttgagata aggtttggct gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct ttggggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacggca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaaca cggcagcgat aaacgtaacc aacgaaatga aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttgcctcct gccatttcgg ctttcggctt ccacaccgaa ccaatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggatgaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttcgaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggtgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc cggtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccgaaa atgctgccgc ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gatcagcaga atttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtctgcac cggactgctg gatgacgggc tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagagcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcag gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gctttccaga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcggcaat cgcattgcgt tccacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tgttgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccggt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt tcccgcctgg
     9001 ataacgacac tgcaatcagc acaggatcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt tccccttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccaggaac gggcagccgc acattccgcg
     9421 aagccgtccg caggcagtgg ctaccccgac ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcatatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAWISC010000064         9770 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWISC010000064 DAWISC010000000
VERSION     DAWISC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.064"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(312..545)
                     /locus_tag="WIW87_001120"
     CDS             complement(312..545)
                     /locus_tag="WIW87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ5174288.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WIW87_001121"
     CDS             complement(711..911)
                     /locus_tag="WIW87_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ5174289.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WIW87_001122"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WIW87_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ5174290.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WIW87_001123"
     CDS             complement(2010..2450)
                     /locus_tag="WIW87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ5174291.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WIW87_001124"
     CDS             2771..3709
                     /locus_tag="WIW87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ5174292.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="WIW87_001125"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="WIW87_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ5174293.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="WIW87_001126"
     CDS             4216..4782
                     /locus_tag="WIW87_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5174294.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRLILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLVFEKLRQK"
     gene            4836..5210
                     /locus_tag="WIW87_001127"
     CDS             4836..5210
                     /locus_tag="WIW87_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ5174295.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="WIW87_001128"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="WIW87_001128"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ5174296.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5840..6319)
                     /locus_tag="WIW87_001129"
     CDS             complement(5840..6319)
                     /locus_tag="WIW87_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ5174297.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6539..7276)
                     /gene="ubiE"
                     /locus_tag="WIW87_001130"
     CDS             complement(6539..7276)
                     /gene="ubiE"
                     /locus_tag="WIW87_001130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ5174298.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7330..7689)
                     /locus_tag="WIW87_001131"
     CDS             complement(7330..7689)
                     /locus_tag="WIW87_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ5174299.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEEK"
     gene            7809..9428
                     /gene="mnmC"
                     /locus_tag="WIW87_001132"
     CDS             7809..9428
                     /gene="mnmC"
                     /locus_tag="WIW87_001132"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ5174300.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQAMQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         2602 a         2984 c         1965 g         2219 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaatcctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca
      301 gacggcattt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtga
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatc ctgaaaaggt
      481 ttgagttcgt cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt cgaggaagat gatactcaag tcctgccaaa
      661 aacagtaaaa atgccgtctg aacagttcag acggcatttc gaaaaccgtt ttacgcttga
      721 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccgc agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgcg aatataaata aaaacggttc gcattgcaag gtcggtatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc aaagtagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcaccctt tcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 aaagccttgt cccgcaaacc gtgcggcgtg tcggtatccg gggcggcaag cagtcgggaa
     1621 atctgctgct cggtgatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccagcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agacgcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaaa actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttctaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaac caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcgtc tgcccgattt cacaatcata ataacggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcacgtt
     3901 ccggtttcga tttacttagt taacggtatc aaattacaag gtcaggttga gtctttcgat
     3961 caatacgttg ttctcctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccgacttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
     4201 agacagcatt taaaaatgtt attgcaaaac atccttccat tcgcccattg ccttttgcgg
     4261 aaggcacttc ccgaaggtgg caatgctttg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga acaacacccg atgccgtctg caggaagcag gttacagcaa tgtacggctc
     4441 atcttggacg gacatgaaaa cctgaagcaa tatattccaa agccgctgga tgcagccatt
     4501 ttcaatttcg gctggctgcc cggcggggac aaaagcctta ccacccgcac ggaaaccagc
     4561 attgccgccc tttctgccac cttatcccta ctgaaagaaa acggtatgct tattgccgtc
     4621 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag caatcgaaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgttttg cgttacggct ttaccaaccg gaaaaacagc
     4741 ccaccctatc ttttggtatt tgaaaaactg cgtcaaaaat aactgtttgc ggtaaaataa
     4801 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
     4861 gccaccaaat cttcgttacc atcaccattt tggtattcaa catccgtttt ttcctacttt
     4921 ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc actgccccac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaaaattac ccatttctcc ccgttcaacg
     5041 cgccttggct cggtacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcctga catccgtctt aaacaggctt
     5341 cctcactgta tatgaccgcg cccgtcggtt acgacaatca gcccgatttt gtcaatgccg
     5401 tctgcaccgt ttccaccact ctggacggca ttgccctgct tgccgaactc aaccgtatcg
     5461 aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acacccgact caccctgccg catccgcgcg
     5581 cgcacgaacg cagtttcgtc atccgccctt tggcagaaat cctccctgat tttgttttag
     5641 gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg caatcaaggt atccgtcttt
     5701 taccggacag gtaattccgc acgcggatgc cgtctgaaag cctttcagac ggcatttttc
     5761 ctttgccgcc aacacgcgtg caaaaaaatc gccccttgga aaagggggcg caaaaggaac
     5821 acaaaccact accaaaactt taaatctgaa acactgcctg ccgcatactg tatccgacag
     5881 gatataaagc cctcactaaa tcgtttcgag aaatccaaac ttcttcatcg ccgacagaaa
     5941 atctgccttt ctccggtacc agctccaaca gaaacggttg aaccgccgta tgcagcctgt
     6001 ccttacaccg ccccagcttt ctggacaacg cggacagggc gcgtttgtag gcattatcct
     6061 tgcagtcaag ctcccgcgca ctgccgaccc agctcaaacg aaggttgcgg ctttcatcct
     6121 caatcagcaa aatgcctgtt ttgacttccc ccacctcggg ctcgcatgaa agcgcaatat
     6181 aatatttgcc gtcgatgttg acatacgccg cttcatgcac ctgacagttg cagccggaag
     6241 tggcaagcag cgaataaaca gaatcccgat gcagtttcaa aatatcaagg gaaatctgtt
     6301 tgaaaatact ttccggcatt tttccctcct ttcgtctgct gcgttgttta ctggattgac
     6361 tgcattatac agacatctcc caatcttgca aatgataatt gttcctattt tttaaaaaat
     6421 ttataaatta ttgatggata tgtgaattaa ttttaagatt taaaatctct ttctcaaaaa
     6481 aacacaaaaa tgccgtctga aacgtgttca gacggcattg gctgcacagc cagtccgttt
     6541 aaaatttcac gcccttatgc agcgcgacga tgcccgcact catattgtga taatccacgc
     6601 tgtcgaagcc cgcatccagc atcatctgtt tcaaagtttc ctgatcgggg tgcatacgga
     6661 tggattcggc aagatactgg taactctccg catctttcgc aatcagcctg cccatgaccg
     6721 gcagcagctt gaacgaatag aaatcatacg cgccttccaa aggtttgtag attttggaaa
     6781 actccaacac cagcaacgtg ccgcccggtt tcaaaacacg gtacatctct ttcagcgcgg
     6841 catctttatg cgtcatgttc cgcaagccga acgccacgga aaccaagttg aaataattgt
     6901 cggggaaagg cagtttttcc gcatcggcaa gcgatacggg caaaatcatg ccttcgttca
     6961 acagacggtc gcgcccgacg gtcagcatag aggaattaat atcggtcagc caaacctcgc
     7021 cttccttgcc gacccgtttc gcccaaccgc gcgacaaatc gcccgtaccg cccgcaatgt
     7081 ccaacacttt atcgcctttt ttcaggtgcg ccgtgttgat ggtgaaatgc ttccacaccc
     7141 tgtgcagccc tgccgacatc acatcgttca taatgtcgta gtttttggcg acggagtgga
     7201 acacttccgc cactttgccg gctttttcat cttcgttgac cgtactgaat ccgaaatgcg
     7261 ttttctgtcc gcccatattc taaccacctc tctgatacct ttgcccgtca gattaccatc
     7321 cgaccggtct tatttctctt caaaacgcga cgcgcccgcc gccgccaatt tgtccaaata
     7381 ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc agatacgccc aatcgtaaag
     7441 accgctgtcg tgcccgtctg aaaaactgat tttcagcgcg tactgtccga caggctgcaa
     7501 atccgcgatt tggacatccg ccttgccggt ctgcaaaaca tcctgtccca cgccgtgtcc
     7561 gcgcacttcc gcactcggcg aatagacgcg taaaaattcg gcaggcagat ttttaggttc
     7621 gtccccataa gtcaaaacca acacggtacg gtttttcaac aaacggattt caacaggaat
     7681 attattcaaa aaacacctcg gttcggcata caatatccgg caggcgcagg cgtgccttca
     7741 aacggcgcgt attttaacag ctttatcccg ataccgatat tcaaacccac actttaaacg
     7801 gcattgatat ggacaatctc gtatgggacg gcattcccga catccgcaca ctcgaccaag
     7861 ccatccgcaa acacgcaccc ccgctcaacc tgattatctg cctccccgat aatcagattc
     7921 ccgattttca aaccgcacaa gatgcttcgg acgcggaatg ccgtctgaag caccgtttgg
     7981 atcaggcaat gcagtgcctc cagttcgaca gcatcaacct catcgaacac atcctgcccg
     8041 atgtccgctt ctggctggtt cccccttcac gcacccacca cctgcacgaa catttccacc
     8101 acatttcctg gcagaccgaa gccatcccgc aaaccgaaag caagcccgac aaaccctggt
     8161 ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca catcctcgtt atcggcgcgg
     8221 gcatatccgg cgcggcaacc gcccacgcct tagcatcaca cggcatttcc gttaccgtat
     8281 tggaagcccg aaaagccgcc caagccgcca gcggcaaccg ccaagggctg ctctacgcca
     8341 aaatctcgcc gcacgacacc gaacagaccg aacttttgct tgccggctac ggctacacca
     8401 aacgcctgct cggacacatc ctgcccgaat ccgaaacctg gggcggcaac ggcatcatcc
     8461 acctcaatta cagccgcacc gaacaacaac gcaatcacga attgggtttg caaaaacacc
     8521 ataaccacct ctaccgcagc atcacatctg cagaagccga aaaaatcgcc ggtatcccac
     8581 tgtccgtccc atacgaccac ccttcatgcg gactctactg gcaacacggc gtatggctca
     8641 atccacccgc attcgtccgc accctcctca accatccgct cattggacta cacgaagaca
     8701 cacccttgac cgacatttcc cacgacgggg aaaagtggat tgcaagcacg ccaaacggca
     8761 catttaccgc cacacacatc atctactgca ccggtgcgaa cagcccctac ctacccgaaa
     8821 ccaacctcgc cgccctgcct ctcaggcaaa tacgcggaca aaccggcctc acaccgtcca
     8881 ccccgttttc cgaacaactg cgttgcgccg tttcaggcga aagctacatc agcccgtcgt
     8941 ggcacggact gcactgctac ggcgcgagtt ttattcccaa cagcagccat accggatgga
     9001 acgaagccga agaagcctca aaccgccaag cattggcaca ccttaacccc gccctttccg
     9061 aatcattgtt tgccgccaac ccaaaccccc aaaaacacca agggcacgcc gccatacgct
     9121 gcgacagccc cgaccacctt cccctagtcg gcgcactcgg cgacattgcc gccatgcggc
     9181 agacctacac caaactcgcg ctggacaaaa actaccgcat cgacacccca tgcccatacc
     9241 tgcctaatgc ctacgtcaac accgcgcacg gcacccgcgg actcgccacc gcccccatct
     9301 gcgccgccgc cattgcagcc caaatcctag gcttgcccca tcccttttca caacgcctgc
     9361 gccacgccct acaccccaac cgcaccatca tccgcgccat cgtcagaagg aaggatctaa
     9421 ccccttaagt ctttgccgca tgccgtcccg accacagaaa atcatattca aataaacata
     9481 ccaatcctga cggtaaaata tcgaaaacac ccataacgtt ttcttcccaa aataaggagt
     9541 aaggaatgcc taacaaattg cctttgctgt ccaagatgaa tgttccgtaa cattcgaaga
     9601 cttccttaac cctgccagcc gtttcggttt ctgtaccccc tgttagataa ggaaggaatg
     9661 aaaaagccct gttgattgta tgaagtaaag ggcgttaatt tttgttaatc ttcccttctc
     9721 aggaactcaa tatataagga tgttaatttt tatttccctt ctcgaggact
//
LOCUS       DAWISC010000065         9519 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWISC010000065 DAWISC010000000
VERSION     DAWISC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.065"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(38..763)
                     /locus_tag="WIW87_001133"
     CDS             complement(38..763)
                     /locus_tag="WIW87_001133"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ5174301.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="WIW87_001134"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="WIW87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ5174302.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="WIW87_001135"
     CDS             1486..1863
                     /locus_tag="WIW87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ5174303.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPMLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RAEGDALQNAAEALRAKQNADKQ"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="WIW87_001136"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="WIW87_001136"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ5174304.1"
                     /translation="MIPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2945..3355)
                     /locus_tag="WIW87_001137"
     CDS             complement(2945..3355)
                     /locus_tag="WIW87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174305.1"
                     /translation="MTTYTIPRKDHQFLYIIEGSLLSYTLKNDEFHIIVNCVDYPDFP
                     QEIPMPNYSDWVRIKFKQFSYLKFIYGYATKSQDKKIKNVLELGELKQDDEILDYGGT
                     LELIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(3421..3603)
                     /locus_tag="WIW87_001138"
     CDS             complement(3421..3603)
                     /locus_tag="WIW87_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174306.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAEPARMGLRL
                     KYNGPLKEEFKQLEFH"
     gene            complement(3611..4765)
                     /gene="lpxB"
                     /locus_tag="WIW87_001139"
     CDS             complement(3611..4765)
                     /gene="lpxB"
                     /locus_tag="WIW87_001139"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ5174307.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARFTGIGGE
                     LMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRTGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4831..5823)
                     /locus_tag="WIW87_001140"
     CDS             complement(4831..5823)
                     /locus_tag="WIW87_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ5174308.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6316..9075
                     /locus_tag="WIW87_001141"
     CDS             6316..9075
                     /locus_tag="WIW87_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ5174309.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEMQTGKRRRNGSRSE
                     RSQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2388 a         3036 c         2251 g         1844 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatcagcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacacgctg ccgatctgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt caccgctttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcggacc gaccgcatac caaaaaaacc
      601 aaccgtcccg agaaaaccac ggaaacacgc cgttccgata cgcctcaagc agccgccccg
      661 tatccaaatc gccgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcttccgcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatacccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct ccttggcctt gccgcgtgca gcgtcgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaaccccg cgccgttgcc
     1621 gccctgcgcc ccggcatgac caaagaccaa gtcctgctcc tgctcggcag ccccatgctg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagaac gcagcaatct gaccgtctat tttgaaaacg gcgtgctcgt acgcgccgaa
     1801 ggcgacgccc tccaaaatgc cgccgaagcc ctccgcgcga aacaaaacgc agacaaacaa
     1861 taaggaacac acatgatacc gctcaaaatc gccatcgccg gcgtcaacgg ccgcatgggg
     1921 cgcgtattgg ttgaagccgt caacaaccat cccgacaccg tcctttccgg cgcgctcgaa
     1981 cactcgggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacaccgtt ctcgcacaaa gcgacgtact catcgacttt
     2101 acccgccccg agccgaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ctatccacac tgccgccgaa
     2221 aaaacaggcg ttgtcttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacctc
     2281 ctcgacaccg tcgcccgcgt attaaacgaa ggctacgaca tcgaaatcat tgaaggtcac
     2341 caccgccaca aagtcgatgc ccccagcggc accgcgttac gcatgggtga agtcatcgcc
     2401 ggcgcgctcg gccgcgacct caaacaatgc gccgtttacg gccgtgaagg ccacaccggt
     2461 ccgcgcgatc cgtccaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgaa cgcgtggaaa tcacccacaa agccagcagc
     2581 cgcatgacct ttgccgccgg tgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggatgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatcaatg
     2761 tttcaacaca caggacgaca cataaagcgt cgccctatgt gttgccctga ttcggaaggg
     2821 gttacgcccc tcccaaataa agtctgattc taccgccccg aaggacagat gtccgagtgg
     2881 cggggtttca accgaaaagg aaatacgata aagttgcttg cacagcaaac aagctacggc
     2941 tcgtttaatt gaaactctcc tgatctaaaa attctaactc tatttcccgg caaactatat
     3001 ctatgctaaa accggtcgga agatcatacc taccgcctat caattccagc gtacctccat
     3061 aatccaagat ttcatcatcc tgctttaatt cgccaagctc caatacattt ttgatctttt
     3121 tatcttggct ctttgtagcg tatccataga taaatttcag atagctaaac tgcttgaatt
     3181 taatccttac ccagtctgaa taattgggca tagggatctc ttgaggaaag tccggataat
     3241 ccacacaatt gacgatgata tggaactcat catttttcaa agtatagctt aatagagacc
     3301 cttcaattat atacagaaat tgatgatctt ttcttggaat agtataagtt gtcatattat
     3361 tttacctttt caaatacaat ggttttaagc ttttttgagt ccaagttggc tccagtactc
     3421 ttaatgaaat tccagttgtt taaactcttc ttttaacggc ccattatatt ttaaccttaa
     3481 tcccattcgg gccggttctg cttcatttgg aattcctctt aaatacttat tgtttgatgt
     3541 gccattaatc acaatttctc cccctttttt tgtaatacgt gctgcttcgg gaaaataatc
     3601 cattaaccgc tcaacatccc gcctcttcca aaaccgcgcg cgcggccaaa tccgccgtat
     3661 cttttttcaa cagcaggtgc aacgccctga aatcctgttg cagcgcggca accttatcgg
     3721 ggtgttcgta ccagtccgcc aacgccgccg ccagtttttc cggtacggca tcatgttgaa
     3781 gcagctccgg cacagcctcc ttacccaaca ggatattcgg caggccgaca tgcggcactt
     3841 tgattttgcg tttcacataa gcataagtca gcggcgaaat cttgtagctg atgaccatcg
     3901 gacgcttgca cagagccact tccaaagttg ccgtaccgct cgttaccagc accgcatccg
     3961 ccgccctgca cactgtttca gactgtctgt cgattaccgt cagcggcaat ccggcaaact
     4021 ccggccgctg caaaacttcc gccaaacgcc gcttcgtcgc cgccgttgcg gcaggcagca
     4081 ggaagcgtgc ggcaggatag cgttccaaca acaataatgc cgtctgaaaa aacaccggcg
     4141 ccatatagtc gatttcgctg acgcggctgc cgggcagcag ggcgaatacg gggatgccgg
     4201 catccacgcc caaagttttc cgcgccgttt cacggtcgtc ttccaagggc ataagctgcg
     4261 ccatcggatg accgacaaac tccgcacgtc cgcccgcatc gagataaagc tgcggctcca
     4321 tcgggaacag gcacagcacg cggttgacct gatgcacgat tttgcccacg cgttcccgcc
     4381 gccacgccca caccgacgga ctgacataat gcacggtcgg aatgcccgtc cgtttcagct
     4441 tttccgccac acccaaattg aaatcgggcg catcgatacc gacaaagaca tcaggtttca
     4501 acgacagcaa atcccgtacc agccccctgc gtatccgtaa aatttccggc agccgcctga
     4561 ccacttcgac aaagccgcgc accgccagcc gctcctgatc ataaaggctc tcgaaacctt
     4621 ccgccttcat cagttccccg ccgataccgg taaaccgcgc ctgcgggcaa cgctcgcgga
     4681 tggcgcgtat taggtgcgcc cccaacaggt cgcccgacgc ttcgccgaca ctgacggcaa
     4741 tcaaagggct ttttttatca accatattcg ttcttcttca catactttca cgtcccgatg
     4801 ccgtctgaag gcttcagacg gcatcggggt tcacattttc gtcccgtttt ccaacatcac
     4861 cgcaaactgt gccaaatccg gcggcagccc cacctttaac accagcggct cgcccgtgag
     4921 cggatggttc aggtgcagct cggacgcgtg caaaaacatc cgcttcaaac ccaacttctg
     4981 caaacgacgg ttcgcctgat aatcgccgta gcgttcgtcg cccgcaatcg gacagccttg
     5041 agattgcagg tggacgcgga tttggtgcgt gcgccccgtt ttcaacgtcg cccgcaccaa
     5101 agtcaggtgc gacagcccga caccgtgcaa aatgccgtct gaaaaacggc ttaacacacg
     5161 gaacaccgta tgcgccgact gcccgtccgc actgacgcgc accatctttt cgccttgtgc
     5221 gccggtatat ttgaacaggg gcagtttgac atggaaattg tcgtccggca gtttgcccac
     5281 ccccagcgca aggtagattt ttttggggtg gtcgttacgg atggcttcgt gaagtttgac
     5341 gagcgcgctg cgtttcttcg ccaccatcaa caagccgctc gtatccttgt ccaaacgatg
     5401 aaccaactcc aaatacttcg cctccggacg ggcgcggcgc aactgttcga taacgccgaa
     5461 actcacgccg ctgccgccgt ggacggcaac gccggacggt ttgtcgatga ccaaaagcgc
     5521 atcgtcttcg taaacaacgt caaacgcacg cgccggtacg gcggcacgcc tttcagacgg
     5581 catttccttc tccgccacgc gcacaggcgg aatccgcacc gtatccccct ccgcaatacg
     5641 gctgtcgggt ttgcagcgtt tcttgttcaa ccgcacctcg ccggcgcgga taatgcggtg
     5701 gatatggctc ttgggaacac ccttgaggat ttttatcaga tagttatcaa ggcgttgacc
     5761 cgcctcatgt tcggcaaccc ctatcaagct gaccgaatct ttgcttattt cgtgcgtttt
     5821 catctataat ccgaacatcc gtttcagcaa aaagcgcgcc cgccgcgcct cctgaaacgg
     5881 tttactttaa aacggaattt tatattaaaa acgcactccg caaagcattt tccctgcgcc
     5941 tgttcccagc cggcaggcgc agaacgtaat caagtttgaa ttgattttgc cgtttcggcg
     6001 cggaagtaag acggcagccg ggcccaagtc ccaaacgcaa accgcccaaa acacaggcat
     6061 cacgataaca agaagaagcc ggcccccatt tttccgatcg cagcgttcgg aacgcggcag
     6121 acaaaccgcc gcatcgatct ttatagcctt ctgtatccga cccttccgca cacggttctc
     6181 cggaacgtgc atccctgcgg attttcaact caaaaactga ttaccggttt tatccgaaaa
     6241 catcggaaaa cccagcctta cggaatgccg aaccgggcat gtgccgtctg aacgccgcct
     6301 gcccacgagg tgatcatgaa aagaatgtta tttaacgcaa cgcaggccga agagctgcgc
     6361 gttgccatcg tcgacggaca aaacctgctg gatttggaca tcgaaacgct gggcaaagaa
     6421 cagcgcaaag gcaatatcta caaaggcatc attacccgca tcgagccgtc gctggaagcg
     6481 tgtttcgtgg attacggaac cgaccgccac ggcttcttgc cgtttaaaga agtctcccgc
     6541 tcatacttcc aagactacga aggcggacgc gcgcgcatcc aagacgtgct caaagaaggc
     6601 atggaagtca tcgtccaagt cgaaaaagac gagcgcggca acaaaggcgc cgcgctgacc
     6661 accttcatca gcctcgccgg acgctatctg gtattgatgc cgaacaaccc gcgcggcggc
     6721 ggcgtatccc gccgtatcga aggtgaagag cgtcaagagt tgaaagccgc catggcggaa
     6781 ctcgacattc cgaacggcat gagcatcatc gcccgtaccg ccggcatcgg ccgcagcgcg
     6841 gaagagttgg aatgggactt gaactacctc aaacaactct ggcaggcgat tgaagaagca
     6901 ggaaaagcgc atcacgaccc ctacctgctc tttatggaaa gctcgctgct gatccgagcc
     6961 atccgcgact atttccgccc cgacatcggc gagattttgg tggacaatca agaagtttac
     7021 gaccaagttg ccgagttcat gagctacgtc atgccgggca atataggccg tctgaaactc
     7081 tacgaagacc acacgccgct gttttcccgc ttccaaatcg aacaccaaat cgaaagcgcg
     7141 ttttcgcgca gcgtcagcct gccctccggc ggcgcgatcg tcatcgacca taccgaagcc
     7201 ctcgtctcca tcgacgtgaa ctccgcacgc gccactcgcg gcgcagacat tgaagacacc
     7261 gcgttcaaaa ccaatatgga agccgccgaa gaagtcgccc gacaaatgcg cctgcgcgac
     7321 ttgggcggct tggtcgtcat cgacttcatc gacatggaaa accccaaaca ccagcgcgat
     7381 gtggaaaacg tcctgcgcga cgcgctcaaa aaagaccgcg cccgcgtgca gatgggcaaa
     7441 ctctcccgtt tcggactttt agaattgagc cgccaacgtt tgaaaccggc tttgggcgaa
     7501 agcagccacg tcgcctgtcc gcgctgcgcc ggcaccggcg tgattcgggg catcgaatcc
     7561 accgccctgc acgttttacg catcattcaa gaagaagcga tgaaggacaa caccggagaa
     7621 gtgcgcgcac aagtgcccgt cgatgtcgcc accttcctgc tgaacgaaaa acgcgccgag
     7681 ctgtttgcga tggaagagcg tttggatgtc aacgtcgtcc tgattccgaa catccacctc
     7741 gaaaatccgc actacgaaat caaccgcatc cgcaccgacg acgtagaaga agacggcgaa
     7801 ccgagctaca aacgcgtcgc cgagccggaa gaagacgaat ccgccaaacc gttcggcggc
     7861 gaaaaagcca aagccgcccg tcccgaaccc gccgtcaaag gcgtgcgcca caccagcccc
     7921 gccccgactg ccgcccccga gaaaaaaacc tcttggtggg acagcttcaa agcatggctc
     7981 aaacgcattt tcggcggcag cgaaacccaa gccgcgcccg ctgccgaaac ctccgaaaaa
     8041 cgcagcacgg caaaccgcag cggcagccgc gccaacaacc gccgccaaaa cccgcgccgc
     8101 agcaaacgcg aaggcagcaa agtagaagtc cgcgaagtgg caggcaaaac tgccggacag
     8161 gaagcgcgtg ccgacaaagc cgaaacgcgc aacaacggca accgccgccg caatgaacgc
     8221 ggcgaccgtg ccgccgaacg cgccaacgaa gcggaaatcc aaagccgcaa cgtacagcct
     8281 gccgcaaccg ttgcagatgc cgcaccgtcc gaaaccgaaa tgcaaaccgg caaacgccgc
     8341 cgcaacggca gccgcagcga acgcagccaa accgcgccgg aaaccgccgc cgttgccgaa
     8401 acagccgttc agacagcgga aaacacgccg tccgaaccgc ataccgcaga agacaaaggc
     8461 agcaagccca aatccgaacg caaccgccgc gaacgcgaca gccgcgacgc caaagaacgc
     8521 cgcgagcgca acaatcagcg cgaccgccgt caaaacggca aaaaacgcaa tatcccgtct
     8581 gccgccaaaa tcgagcagta cctgaatatt cacgacaccg ccgacaaagt ccgttccgcc
     8641 gccgcgcacg ttttcggcga aaccgacgca aacgcgccga ttaccgtcag cattgccgat
     8701 ccggttgcag aaagggatct tccgacagca tctcccgccg tttcaaacgg cgacgcaccg
     8761 gtttatgatg cggcggaaaa aatccgccgt gccaccgccg ccatcctgcc cgaaggcgcg
     8821 acaccgaaag ccgaagcaca ggaaatgccg tctgaaaccg caacctttac ggctgcggcg
     8881 gaacaggcac gggaaaccgc acaaaccggc ggactcgtcc tgatcgaaac cgaccctgcc
     8941 gcattgaagg catgggcggc acaacccgaa gtccaaaccg gacgcggttt gcgccgttcc
     9001 gaacagccca agccgtctga agtcgcaacc gtccctgccg aagaaatgat ccaagtcgaa
     9061 acccggcaag gctgaaccga cggcggcaaa aagaggttct gttccgcaga acctcttttt
     9121 tatatagatt cggatacctg caatgccgtc tgaagcctca ccattcccat gattaccgca
     9181 acctttcgtc attcccgtga aaacgggaat ctagaacctc aaactttcaa ataacctttg
     9241 aatattgccg ctgccttaag gtctggattc ccacctgcgc ggaatgacgg tttagaagtt
     9301 gcccgaaacc tcaaaaaaaa ctgaaaccga acaagccgga ttcccgcaac attccgtcat
     9361 tcccgtgaaa acgggaatat agaaccccaa actttcagat aatctttgaa tattgccgtc
     9421 gcccaaaggc ctggattccc gcctgcgcgg gaatgacggc ggagggtgga cgatgccgtc
     9481 tgaaacttcg ccattcccat gattaccgca acctttcgt
//
LOCUS       DAWISC010000066         9470 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWISC010000066 DAWISC010000000
VERSION     DAWISC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.066"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            89..298
                     /locus_tag="WIW87_001142"
     CDS             89..298
                     /locus_tag="WIW87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ5174310.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="WIW87_001143"
     CDS             387..1601
                     /locus_tag="WIW87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ5174311.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIW87_001144"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIW87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ5174312.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRS
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="WIW87_001145"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="WIW87_001145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ5174313.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNLDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDTPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5607..6152
                     /locus_tag="WIW87_001146"
     CDS             5607..6152
                     /locus_tag="WIW87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174314.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVIRLIGKHPGDLEAVSGKCME
                     TDDKDSPAGWAENGVCHTLFAKLVGNIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6472..6795
                     /locus_tag="WIW87_001147"
     CDS             6472..6795
                     /locus_tag="WIW87_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174315.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WIW87_001148"
     CDS             complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WIW87_001148"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ5174316.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8629..9360)
                     /locus_tag="WIW87_001149"
     CDS             complement(8629..9360)
                     /locus_tag="WIW87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5174317.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2200 a         2444 c         2500 g         2326 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt ttaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg cgcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgacg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc ccttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcatt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac tatcccttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgaca
     1021 aaatccttta tgacggcgcg gttcaccacc acatcgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaagcct accgtgtagc cggattccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcatgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtctatgcg cctgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gctatcagag catcaacgaa ttcattttgc ccggcggacg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcaac cagattcccg gcgtatcctg cgtcaaaccg atgggcgcga
     1381 tgtatatgtt cccaaaaatc gataccgaaa tgtaccgtat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ccatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gtgcggcatc ggaaacagcc aggcgcaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcggggc ggaaatgttc tttcacatcc tccatcacaa
     2041 cttctttcac cgcttcgtaa tcctgaatgc ccatttgttg gatatgttgg ctaccaatat
     2101 tggaagtcat cacgataacg gtatttttga agtccacggt gcgaccttgt ccgtcggtca
     2161 agcggccgtc atccaatact tgcagcagga tgttgaacac atcgggatgg gctttttcca
     2221 cttcgtccag cagaatcacg ctgtacggtt tgcggcgcac ttgttcggtc aggtagccgc
     2281 cttcttcgta gccgacatag cccggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atgcgaatca gatgatcttc gctgtcgaac agaaagcctg
     2401 ccagggcttt acacaactcg gttttaccca cgccggtcgg gcccaagaac aggaagctgc
     2461 cgtaaggctt gttcggatcg gcaagaccgg agcggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcctgt ccgaccacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctctgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtctaaatcg gcgtattctt tttgcagacc gttgatttcc tcgtctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcgggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccatggtg gatttcataa cgctcctgta aaccgcgcaa aatagcgatg gtgtcttcca
     3241 cgcttggctc gccaaccaat actttttgga agcggcgttc gagtgccgca tctttttcga
     3301 tgtattggcg gtattcgtcc aaagtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcgtcca tcgcgccgtc ggttttgccc gcgccgacca
     3421 aagtatggat ttcatcaatg aaaatcagag tgttgccgtc gtctttcgcc aaatcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaaagccg
     3541 ccaaatccaa aaccagcaag cgtttgttac gcagggattc aggtacttcg ccgttgacga
     3601 tacgttgcgc caagccttca acaatggcgg ttttacccac acccggctca ccaatcagca
     3661 cagggttgtt tttggtacgg cgttgcaata cctgaatcgc gcggcggatt tcgtcgtcac
     3721 gaccgataac ggggtcaagt ttgccgtcgc gggcgcgctg ggtcaggtca agcgtatatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ctgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggtcgcatc gttctgctgt accaaggcaa gcaggaaaag ttcgctggca atataggcat
     3961 cgctgcgttt ggtggcagct ttgtccatca ggttcaacac cgcctgcaat tctcggctgg
     4021 gcagaatatc gccgccctga ccggacactt tcggcaggct gtttaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg catgagccaa gagcgcggcg gctccgctgt
     4141 tttggtcgtc aagcagggct tttaacacaa agcccgcttc cagatagctg ccgtccgcag
     4201 ccaacgccaa actctgagct tctgcaaggg cttgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc acatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggcgcca tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc tcgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacactcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcact gccaagggtt tgatgaaccg cgcccatgcc
     4861 tacaaagccg ccgtgcaggc aaatgcagaa aacgggcagg aagaccctga tttcggtgtg
     4921 gaaatgggtt tgttcagtta tccgattctg atgactgccg atattctgat gttcaacgcc
     4981 aacgaagtgc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca accaccgctt ccgggaactc ttcaccctgc ccgaagtgaa aatcgatgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aacggtggaa tttacgaaaa tgcttgccga cggcttggcg
     5341 tggggtgaag ccaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt gcaggcaggc
     5461 gcggcgaaag cgcgtaaaga agcacgcgaa ttattggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaatgaac ccgatgccgt ccgaacctcc gcctgccgcg tttcagacgg
     5581 cattttgaaa ccatcaggag tgtggaatgc tgaaaacatc ttttgccgta ttgggcggct
     5641 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaacgcgg
     5701 tacaaagcgc gccgaaaccg gttttcaaag tcaaatatat cgacaatacg gcgattgccg
     5761 gtttggattt gggacaaagc agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
     5821 atccgattaa aggcttgccg gaacaaaatg ttatccgact gatcggcaag catcccggcg
     5881 acttggaagc cgtcagcggc aaatgtatgg aaaccgatga taaggacagt ccggcaggtt
     5941 gggcagaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
     6001 acggcggcaa actgacggat tacctagttt cgcatgccgc cctgcaaccc tatcaggcag
     6061 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgaag
     6121 gggcgtttta tttccgccgc cgccattatt gaggtattcg gacatcccgg aatatatttg
     6181 ggtttttcaa accctgcagg aaaaagtccg caccgtcggg aaactcaaaa ggagggggat
     6241 atgtgttaca attttccgaa ctgttttcat aaaatagttt tcggacgtgt ttcaatatgg
     6301 cattgatgcc gccttattgt tcgagaaaaa aacctttata tttaaatata atgggtttta
     6361 actaaacggg aaaccgtttt ctctccggtc gatgggcaaa atcagccgat tgatggaaca
     6421 cggtccgatt tttaagcaaa accttaatca acaactcaag aaggataaga catgaaaaaa
     6481 ttattgattg ccgcaatgat ggcggctgcc ttggcagctt gttcgcaaga agccaaacag
     6541 gaggttaagg aagcggttca agccgttgag tccgatgtta aagacactgc ggcttctgcc
     6601 gccgagtctg ccgcttctgc cgtcgaagaa gcgaaagacc aagtcaaaga tgctgcggct
     6661 gatgcaaagg caagtgccga ggaagctgta actgaagcca aagaagctgt aactgaagca
     6721 gctaaagata ctttgaacaa agctgccgac gcgactcagg aagcggcaga caaaatgaaa
     6781 gatgccgcca aataatttgt tgccttggca aaaatgatgg gatgccgcct gccggcaacc
     6841 caaacgaaac cgcctgaaga ttttcaggcg gtttttgggt tatgtgcccc ttgtttttta
     6901 cgcctgcatg accgttccga gcagatgtgc cgcgcatatc gggatgttgc ggacaggcaa
     6961 atgccgtctg gacagggttt ggacggcatt tgttccgcaa ggttcagacg gcctcgactt
     7021 tgaacggcat attgattttt tgccattgga cgctttcata ttccaatgcg tcggcaatca
     7081 ggggatggcg ttccagccat tccctgtcaa tacgcaggat gaagccgcag ctttccgtat
     7141 ccgtgcgcaa ctgcatattt ttcgggaaag acaggtcttg gcgcgaacgg cagaacagtg
     7201 cggcaaggcg cagggacaaa acggcatacc acaacatttc gttggtgccg atgatgccgc
     7261 tcattttttt catatcgccg cgatgaccga tgaccagttg ggcaagtatg gtctgttctt
     7321 tgcgtgagaa acccggcata tcggcgtttt cgaggatgta ggcggaatgc ttgtgatagc
     7381 cggtgtgggc gatgtccaaa ccgatttcgt gcagcgcggc ggcgcgtccg agatactgtt
     7441 gccacaaggc aagctcttga actgtaacgt ttttagcgtg gcagaggctg tccataaagg
     7501 tttgcgcggt ctcggcggtg cgtttcgcct gattgaggct gacgtggtag cggtgttgga
     7561 actcggcaac cgtttgtccg cgcatatctt cgtttaaacc gcgcccgatc aaatcgtaaa
     7621 acacgccgtc gcgcagggcg gcttcggtta cggtcatcct gtcgagtttc atttcctcaa
     7681 acgccgccat catcacggca agtccgccgg caaaaacttc gatgcgttcc ggtttcaggt
     7741 tttcaaattt ggcttttttg accgaaccgg cttcgatgat gcgttcggcg agggcgcgca
     7801 tgcctttgta ggtaatgtcc gcctcttggg gcatttcggc ggcaagcacg tcgcggatgg
     7861 attttgccga acccgatgtg ccgacggcga aatcccaacc ttcgcgcctc atatttttgc
     7921 tgatacgctg gatttcgttg cgggcggcgg aaatggcaga ttggaagtct ttggcggtga
     7981 ttttgttttg gaagaagcgc aggctgtagg ttacgcagcc caagggcagg ctttcggtaa
     8041 tgtcgggatt cagcgtcgag ccgatgacaa attctgtcga accgccgccg atgtcgataa
     8101 ccagcatttt gccgccgccc ggggggaggg tgtggatcac gccggtataa atcagccgcg
     8161 cctcttcgcg cccggcgatg atttcgatgg ggaaacccaa tgccgcttcg gctttgggaa
     8221 ggaaatctgc gatgtttttg gcaacgcgga atgtgttggt tgccacggcg cgtacctgtt
     8281 cagggcggaa gccgcgcagg cgttcgccga attttgccag acagtccaaa gcctgttctt
     8341 gggaagcggc actcagattt ttctgttcgt ccagtccggc ggcgaagcgc accatctgtt
     8401 tgaacgaatc gatgactttt aattgtccgt tgttgttttc gcaaatctgg aggcggaaac
     8461 tgttggaacc caaatcgacg gaggcgagga cgtttgcggg ggtggtggtc atggcggata
     8521 ccggtggggg aaaaacgcaa tgttactctg acggcgcagg cgttgacaat aaatgatgcg
     8581 gcggtttttg attctgccca cggatgttgc cgacggcatt ttttgcgctt atttgaaatc
     8641 cttttccacg ctcatgaaaa tctgcatgtt tttgcgtgtg taaaaacttt tcatattgct
     8701 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
     8761 atgccacaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
     8821 ggtcccgggt gcgtcaaaca tcctgcgggt atagcgcagg tttgcccgca gacccaagcc
     8881 gccgtcgaac gttttgaccg cgccgacacg caaacccttg cggatggaag cctgttccgc
     8941 ctctttcgtt atgttgtgcg accagtccgc accgccgtaa agcagccagt ctttcggcgc
     9001 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
     9061 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
     9121 caagcgttgg gagaaatcgg cattgaagcc gccgacgaaa ttgtatcggc tgccgcctaa
     9181 gaggttttgc tcgacaaacg gcacgatgcc gaacgagcgc gttaccgaac ggtttttata
     9241 gccgaacgac aggcgcaggc tctgttcgct gaaatctttg ttatcccaat aatgcacgcc
     9301 gccgccgctg atgccgccgt agaggaaatg atgcctgccc gcattgattt cgcgcgacac
     9361 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
     9421 gcttctgccc gcaaattcaa tcgttttttc ggacgaagcg ttgtttatag
//
LOCUS       DAWISC010000067         9369 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWISC010000067 DAWISC010000000
VERSION     DAWISC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.067"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..190)
                     /locus_tag="WIW87_001150"
     CDS             complement(<1..190)
                     /locus_tag="WIW87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174318.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESE"
     gene            complement(208..765)
                     /locus_tag="WIW87_001151"
     CDS             complement(208..765)
                     /locus_tag="WIW87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ5174319.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(841..2475)
                     /locus_tag="WIW87_001152"
     CDS             complement(841..2475)
                     /locus_tag="WIW87_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ5174320.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2549..2878)
                     /locus_tag="WIW87_001153"
     CDS             complement(2549..2878)
                     /locus_tag="WIW87_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174321.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WIW87_001154"
     CDS             complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WIW87_001154"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ5174322.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMSDYNGTGFSVMEMSHR
                     SDMFLSILHHAEQDLRQLLNIPSNYKILFLQGGATTQFNMAVLNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVSDYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSDGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4349..4780
                     /gene="rimP"
                     /locus_tag="WIW87_001155"
     CDS             4349..4780
                     /gene="rimP"
                     /locus_tag="WIW87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ5174323.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            4808..6310
                     /gene="nusA"
                     /locus_tag="WIW87_001156"
     CDS             4808..6310
                     /gene="nusA"
                     /locus_tag="WIW87_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ5174324.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6322..9210
                     /gene="infB"
                     /locus_tag="WIW87_001157"
     CDS             6322..9210
                     /gene="infB"
                     /locus_tag="WIW87_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ5174325.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2503 a         2691 c         2377 g         1798 t
ORIGIN      
        1 cttctgattc cccgataccg atgccgtctg aaccttcaga cggcatgaga cctttgcctg
       61 cgtacttggt gcgctggtcg cctccgaaca tggcgcgaca cccgacattt cccccgaacg
      121 catcgagcgt ttcatgaatc cggtttaaaa cgcatggaaa aatgccgtct gaaagccttt
      181 cagacggcat ttcgcttgag attccgttta ccaatggctg acaaacgctt ccaaatcggt
      241 attcttgggc ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc cgatgatttt
      301 atccttatcc gcacatccga aagcctcacg caacaggggg ctgttgaccc acatccccgt
      361 aatccagaca ttgtcgaaac cttgcgccgt tgccgccagt tgcagcgcat acgccgcaca
      421 acccgccgtc agcatctgct cccattccgg tttcggctta ggcacatcgc ggttcggcgc
      481 aaacgttaca ccaatgacca tcggcgccat attgcccact ttttccgcct ttttcatcgc
      541 atcgtcgccg aaattcaatt cggcaaccgt ttgctttaac acatcgcgaa aacgttgcaa
      601 tcctacctcg ccttgaatca cggtaaaacg gaaggggcgc atattgccgt gatcgggaac
      661 ttgggttgcc gcctgaaata tttgttccaa ctccgccgca tcgggggcgg ggggcttcag
      721 ctttttggaa gatcggcggt tcgtcaataa ttttaaagca tccatatcgt tatactccgg
      781 tcatcagtcg ggtgttcgga cacatatgcc gtccgaaggc ttcagacggc atatccggca
      841 tcagcgcgga cggcggcagg ctgccaatat atccatttcc ttccgatagg tttggctgtt
      901 ggaaatgtcc atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc
      961 cgccgctttt tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga
     1021 aaaccacata accatcggga tatgcgtctg cccggaaggc gcgatggcgt aaggcgcggc
     1081 gtgcaggtac atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatgcaccac
     1141 gctttccaaa tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actggtccac
     1201 atacaaaacc gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt tgttgatttc
     1261 gttggtgtcg caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt cgtaatacgt
     1321 cggcccgtgg ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag
     1381 gacttcgtcg aacttagtca gcaggatatt gtcgaggcac tcgccgttgc ggcagtattc
     1441 gggcaggttg agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc
     1501 ggaatcgttt tccaaccagg taacttccac gccggcgcgc tgcacgatgt ccagcaggtt
     1561 gtcttggtgt tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca
     1621 cggcagggag tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc
     1681 atcgccgcgc gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt
     1741 ggcggcacgc gtggtctcgc ccacgaccag caccacgaaa cggcgcaggc tgccggccgg
     1801 ccggttttgc acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc
     1861 tttgtatttc gacacgcccg cgccgatgaa attagacggc acaatcagat gggttactga
     1921 tttattgttg cggaaaaacg aggcgtaatc ctgatattgc aacattgcga tgcccaacgc
     1981 gcacaaaaag gaaacggcgg caagcacaag gcgcgtcaaa agctccttat accaaacgcg
     2041 gtatttaacc ttgacggcga tatacgccag cgcgggcaat acgcccaaac atacaatcca
     2101 cagcacatag cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt gcaagacatt
     2161 attcaacatc gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc
     2221 actgataacc aatatcaacg gaatcaatac cttatgcacg aaaggcaggg caatgacgtg
     2281 aaaaacgaaa ttacttaaaa aaaacagcac caccggcatc gtatagagga agatatccgc
     2341 cccggtgccg ttaaaaggat gaagctcgac aactttggca aaaaaggcgt aattcaatac
     2401 cagcgaggaa tacagggaaa ggaaggcaat cagcgcggaa gagccgagct tcggcctcag
     2461 gttcggtttt atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggcatttgg
     2521 aatccggatt attgaaaaag attcttaatt ataaggcaac ggagcaaagc agggcaagaa
     2581 aacggcggct gtgcgggggg ttccgcccgc cattcaaacg tccggcagac ataaaaacat
     2641 cgtaagcaag attcgaaccg gtctgcaacc gcccctgcca gaaaaacggg caaagcattt
     2701 catattggaa aaacccggcc gcgccgccga cgggacagtc cggcacaaac agcatcacgc
     2761 ccggattgaa aaggacggat agccgccggc agcacaatct taccacctcc aaaacgccgc
     2821 cggagaacgc cgaaacagcc gatgccgtct gaagccgctt cagacaacat cgggacatca
     2881 accgtaacgc cgttggaaat cgcgcataaa atctgccaaa gcccgcacgc cttcaagcgg
     2941 catcgcatta taaatgctgg cacgcatacc gccgacggtt ttatagccct taagcaggca
     3001 caagccctgc aattcggctt ccagcacaaa acggcggtca agctcctcat cccccgtttg
     3061 gaacacgaca ttcattttag aacgcgcatt cggacggata cggttgatat aaaaaccatc
     3121 gctgccgtct atcgtctcat acaaggtttg cgccttcagc cgattgaccg cttcaatttt
     3181 tttcacaccg ccctgcgcct gtagccagcg gaacaccagc cccgacatat aaatcgcgta
     3241 agttgacggc gtgttgtaca taccgtcgcg gttcaaatgc gaacggtagt tgaacacatc
     3301 gggaatatcg ttcggacaac gctcgagcaa atcctcacgc acaatcacca ccgtaactcc
     3361 tgccggcccg atgtttttct gtgcgcctgc gtaaatcaag ccgtaatcgg acacatcaaa
     3421 ctcgcgcgac agaatctcgc tggacatatc gcacaccagc ggcggcatgc cttctgaaag
     3481 gcgcggcact tcacggtatt gcagcccgtt gaccgtttca ttgacggcaa aatggacaaa
     3541 cgccgaatcg ggtgcaacat cccacgtttc cacaggcggc aggtcgagat agtcgaactg
     3601 ctcgccgcca tgcgccgcca aacggatttc cgtatcggtc aaacggctca tctgttcata
     3661 agcgatacgg ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac cgtgtgccag
     3721 attcaaaacc gccatattga attgcgtggt cgccccgccc tgtaaaaaca ggattttata
     3781 gttggacgga atgttcaaaa gctgccgcag gtcttgttcg gcatgatgca ggatgctcaa
     3841 aaacatatcc gaacggtggc tcatttccat cacggaaaaa cccgtaccgt tgtaatccga
     3901 catttcctgc cgcgccgttt ccaacacggc ttcgggcaat acggcagggc cggcggaaaa
     3961 attgtaaatc ggataaagag acatgatgca gccttgattc tgaacaataa cccgcctgat
     4021 tttaggcttg cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg ccccgcttca
     4081 tgccgcccga acacggaaaa caacatcaat atattgattt acaaatataa aaatcatgct
     4141 agcgacaaat agatacattt gttttgtcaa caatattcac gatttcccat tacaaacctc
     4201 ccttacaccc gcttttttcc gtcccaaaaa cacaaaataa atcaacactt tcatttctcc
     4261 gcaaaagcgg ttataatcac gccgattttt caactttgac gaaaaatggc cacggggcca
     4321 tttttttatt atgtacatag ggagcagcat ggatatccaa accatcctcg aaaaaaccct
     4381 gcccggcctg ggctacgaac tggtcgattt tgaactgacc gcgcaaggaa cattgcgcgt
     4441 gttcatcgat aaagaaggcg gcattaccgt cgaagactgc gcaaccgtca gcaaccactt
     4501 gagccgcgtc ttcatggttg aagacatcga ctacaaaaac ctggaaattt ccagccccgg
     4561 actcgaccgt cccttgaaaa aagccgccga cttcgtgcgc tttgccggtc agaatgccaa
     4621 aatcaaaacc cgcctgccga tagacggtca gaaaaacttt atcggtaaaa tcgaaggctg
     4681 cgaaaatgat accgttacca tatccttcga cggcaaaacc gtacaaatcg aattgggcaa
     4741 catcgacaaa gcccgtctgc gccccgaatt caaattctaa aacacagcaa tattggagat
     4801 gttcaaaatg agtcgtgaaa tgttacagct ggcagaagca ctggcaagcg aaaaaaacgt
     4861 tgatgcggaa gtcgtcttcc aagcactgga attcgccctg tctaccgccg ccaagaaaaa
     4921 ggcagaccgc gaacacatgg atgtccgcgt acaaatcaac cgcgacaccg gcgaatacca
     4981 aaccttccgc cgctggctga ttgtcgccga tgaagactat acctatcccg atgtcgaaaa
     5041 aaccatcgag gaaatccaag aggaaattcc cggcactacc atccaaatcg gcgaatacta
     5101 cgaagagcag ctgcccaacg aaggcttcgg ccgccaagcc gcgcaaaccg ccaaacaaat
     5161 catcctgcaa cgcatccgcg atgccgagcg cgagcagaat ctgaacgagt ttctcgccgt
     5221 caaagaagac atcgtgtccg gcacggtcaa acgcgtcgaa cgccacggca tcatcgtcga
     5281 agtcgttgcc ggcaaactgg acgcgctgat tccgcgcgac caaatgattc cgcgcgaaaa
     5341 cttccgcagc ggcgaccgca tccgcgccct cttcctgcgc gtcgaagaaa tcggcaacac
     5401 cggccgcaaa caagtcatcc tgagccgtac ttccggcgat ttcctcgtca aactgtacgc
     5461 caatgaagta cctgaaattg cagacggcat gcttgaaatc cgcgctgtcg cccgcgaccc
     5521 gggccagcgt gccaaagtcg ccgtcaaagc caacgaccag cgcatcgatc cgcaaggcac
     5581 ctgtatcggc gttcgcggtt cgcgtgtcaa tgccgtcagc aatgaattat ccggcgagcg
     5641 catcgacgtt gtcctttggt cgcccgagcc tgcccaattc gtcatgagcg cgctctcacc
     5701 cgccgaagtc agccgcatcg tcatcgacga agacaaacac gccgtcgatg tcatcgttgc
     5761 cgaagaccag ctcgcgctcg ccatcggacg cggcggtcaa aacgtgcgcc ttgcttccga
     5821 cctgaccggc tggcagctca acatcatgac ttccgccgag gcagacgaac gcaatgcggc
     5881 agaagatgcc gccatccgcc gcctgtttat ggattacttg aacgtggacg aagaaaccgc
     5941 cgacgtactg gttcaggaag gttttgcaac cttggaagaa gtcgcctatg ttcctgccgc
     6001 cgaactgctt gccattgaag gatttgacga agaaatcgtc gatatgctcc gcaaccgcgc
     6061 ccgcgatgcc atcctgacca tggcgattgc cgccgaagaa aaactgggcg aagtgtccga
     6121 cgatatgcgc aacctcgaag gcatagatgc cgatatgctc cgcagccttg ccgaagcagg
     6181 cattaccacc cgcgacgact tggcagagct tgctgtggac gaactgattg aaatcaccgg
     6241 tgtaaacgaa gaaaccgcaa aagccgtcat cctgaccgca cgcgaacact ggtttaccga
     6301 agacaaataa agggggtaca gatgagtaac acaaccgtag aacaatttgc cgccgagctg
     6361 aaacgccccg tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag caaaaacagc
     6421 ggcagcgatt ccctgacgct ggacgacaaa cagcttctga acgcctacct gaccaagaaa
     6481 aacggcagca acggcggtac catcagcatc cgccgcacca aaaccgaagt cagcaccgtt
     6541 gacggcgtaa aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc ttctgccgaa
     6601 gaattggcag cacaggtaaa agccgcccaa acccaagccg cacctgtccg gccggagcag
     6661 acggcagaag acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga agcccgtgcc
     6721 aaggcagaag cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa agccaaacct
     6781 gccgcgcaga aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc ggaaaccaaa
     6841 cccgccgaag aaagcaaagc ggaaaaagcc caagccgaca aaatgccgtc taaaaaaccc
     6901 gccgagccca aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa aggcaaagat
     6961 gccaaaaaac cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa
     7021 gaacaggcgc aacgcgatga agaagcacgc cgtgccgccg cacttcgcgc ccaccaggaa
     7081 gccctgttga aagagaaaca ggaacgccag gcacgccgcg aagccatgaa acaacaggca
     7141 gaacaacagg caaaagccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct
     7201 gccgagaaac cgcaggcagc cgcgccggcc gtcgaaaaca aacctgtcaa tccggcaaaa
     7261 gcgaaaaaag aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc
     7321 aaaggcggaa aaggcggacg cgaccgcaac aatgcacgca atggcgacga cgagcgcgta
     7381 cgcggcggta aaaaaggcaa aaaactcaaa ctcgagccga accaacacgc cttccaagca
     7441 ccgaccgaac ctgtcgtaca cgaagttttg gtgcctgaaa ccattaccgt tgccgacttg
     7501 gcacacaaaa tggcagtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg
     7561 atggttacca tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc
     7621 ggacacatcg gcaaacccgc cgccgccgac gatcccgaag ccttcttgga cgagggcgcg
     7681 gaagcggtgg aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc
     7741 gaccacggca aaacctcgct gctggactac atccgccgta ccaaagtggt acagggcgaa
     7801 gcaggcggca ttacccaaca catcggtgca taccacgttg aaaccccacg cggcgtgatt
     7861 accttcttgg atactccggg tcacgaagcg tttactgcca tgcgtgcgcg cggtgcgaaa
     7921 gcaaccgata tcgtgattct tgtggtcgcc gccgacgacg gtgtgatgcc gcaaaccatc
     7981 gaagcgattg cccacgccaa agcggcaggc gtaccgatgg tggttgccgt caacaaaatc
     8041 gataaagaag ccgccaaccc agagcgtatc cgccaagagc tgaccgcaca cgaagtcgtg
     8101 cctgacgaat ggggcggcga tgtacagttt atcgacgttt ccgctaaaaa aggcctgaac
     8161 atcgatgcat tgctcgaagc agtcttgctc gaagccgaag ttttggaact gactgcccct
     8221 gtcgatgcac ctgccaaagg tatcatcgtt gaggcacgtt tggacaaagg ccgcggcgcg
     8281 gttgccacat tgctggttca aagcggtacg ctgaaaaaag gcgatatgct gctggccggt
     8341 acggcattcg gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc
     8401 ggcccgtcca tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac
     8461 gcgatggtat tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa
     8521 taccgcgacg tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat
     8581 atgggcgaaa cccaggccca atccttgtcc gtcatcatca aagcagacgt tcagggctct
     8641 tacgaggctt tggcgggcag cctgaaaaaa ctgtccaccg acgaagtgaa agtgaacgtg
     8701 ttgcacagcg gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc
     8761 gcattcatta tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat
     8821 gaaaacgtgg aaatccgcta ctacaacatc atctacgatg ccatcgacga cgtgaaggcg
     8881 gcgatgagcg gtatgctttc cccggaagag aaagaacagg ttaccggtac ggtcgaaatc
     8941 cgtcaggtca tctccgtttc caaagtcggc aacattgcag gctgtatggt taccgacggc
     9001 gtggtcaaac gcgattccca tgtccgcctc atccgcaaca acgtggttat ccacacgggc
     9061 gaactggctt cgttgaaacg ctataaagac gatgtaaaag aagtccgcat gggcttcgag
     9121 tgcggtctga tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc
     9181 gacatcgtcg aagttgcccg cagcctgtaa ttcctttgca aataaaatgc cgtctgaagc
     9241 gttcagacgg caacgaaacg ggttctgtat catacagaac ccgttttttg tcgcaaatcg
     9301 gcttcagaca gccctcttgc cttatcccga tttgaatctg acttgccata caaacaggct
     9361 tcagacggc
//
LOCUS       DAWISC010000068         9323 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWISC010000068 DAWISC010000000
VERSION     DAWISC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.068"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(91..1371)
                     /locus_tag="WIW87_001158"
     CDS             complement(91..1371)
                     /locus_tag="WIW87_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ5174326.1"
                     /translation="MENSLKEAALKFHEFPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WIW87_001159"
     CDS             complement(1593..2213)
                     /locus_tag="WIW87_001159"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ5174327.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLKPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRNLDEVRNSIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIW87_001160"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIW87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ5174328.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WIW87_001161"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WIW87_001161"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ5174329.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRHYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFLVDRNAV"
     gene            4142..5428
                     /locus_tag="WIW87_001162"
     CDS             4142..5428
                     /locus_tag="WIW87_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ5174330.1"
                     /translation="MIYIVLFLAVVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5576..8101
                     /gene="ligA"
                     /locus_tag="WIW87_001163"
     CDS             5576..8101
                     /gene="ligA"
                     /locus_tag="WIW87_001163"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ5174331.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     TRHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8158..>9323
                     /locus_tag="WIW87_001164"
     CDS             8158..>9323
                     /locus_tag="WIW87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ5174332.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2185 a         2834 c         2358 g         1946 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacaccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat agcgaacacg atgggttttt cgttcatggt
      541 gttcagtatt tcaggcgtca gcaggttcgc gccggagagg cccaagaaga tgtctttgcc
      601 tttgacggca tcggcaagca cgcgctggcc gttgtcttca atggcgtaga actgtttgga
      661 ctcgtccata cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat agccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttgccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacagc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccagaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 cacggggaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggcggatgc gcttgtttca atatttgccg catcagcagc cgaaatgtcc gtccaacact
     1621 ttttctatgc tgtttctgac ttcatccaag ttgcggttac tgtcgataac ggcgtaccgt
     1681 tcgggacagg cggcggctcg gtcgagataa acgccgcgca cacgcataaa gaaatccgcc
     1741 tgctcctgct cgaaacggtc tttctcgcgc gtctgcccga tacgcgccat cgacacttcg
     1801 agcggcacat ccagcagcag ggtcaaatcc ggcttcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggtcgcg
     1921 tcggtaaaac ggtcagacac gacgtgtatg ccgtctgaaa gcgcgggcag gatgacttcc
     1981 tcgatgtgct gcatacgcgc ggcgaacatc atcagggttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggctcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcggacg gcggcattgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtgcc gtccattttg gacacgaaat acaggtattt ttcgccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg caatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 ccaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagg cggcgttgca tcgccttgta ggcggtttgg taaatctgca
     2761 aatcactgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcgggcgca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgtccgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc aatctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt gattcggtat gccctgccgt
     3181 tatccttagg aacaaaaagc agcgcggcga aaacggctgc cgacacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatacgg cacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttctta
     3601 atcaaacgcg acggagaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatctt cattcggcgg cagggaaaaa tgcaacgcat tttcaatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc gtttgccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccacta ccccgtcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg ccattttttc gactggcggc ggatacggga gaaagggttt
     3901 ctcgtggaca gaaatgccgt ctgaaaacca cagatttcag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 atcggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tgcaagcccg
     4081 atttaaccgc agtacccgca ctttgcgtat cctgcatccc gaaatttaac aagtgattgc
     4141 catgatttac atcgtactgt ttctagctgt cgtcctcgcc gttgtcgcct acaacatgta
     4201 tcaggaaaac caataccgca aaaaagtgcg cgaccagttc ggacactccg acaaagatgc
     4261 cctgctcaac agcaaaacca gccatgtccg cgacggcaaa ccgtccggcg ggtcagtcat
     4321 gatgccgaaa ccccaaccgg cggtcaaaaa aacggcaaaa ccccaagacc ccgccatgcg
     4381 caacctgcaa gaacaggatg ccgtctacat cgccaagcag aaacaggcaa aagcctcccc
     4441 gttcaaaacc gaaatcgaaa ccgccttgga agaaagcggc attatcggca actccgccca
     4501 caccgtttcc gaaccccaaa ccggacattc cgcaccgaaa cctgccgacg cgccggcaaa
     4561 acctgcaccc gttccgcaaa cacctgcaaa accgctgatt acgctcaaag aactgtcaaa
     4621 agtcgaatta ccctggtttg acgtgcgctt cgacttcatc tcctatatcg cgctgaccga
     4681 agccaaagaa ctgcacgcac tgccgcgcct ttccaaccgc tgccgctacc agattgtcgg
     4741 ctgcaccatg gacgaccatt tccagattgc cgaacccatc ccgggcatcc gctatcaggc
     4801 atttatcgtg ggtattcagg cagtcagccg caacggactt gcctcgcagg aagaactctc
     4861 cgcattcaac cgccaggtgg acgcattcgc acaaagcatg ggcggtcaga cgctgcacac
     4921 cgaccttgcc gcctttatcg aagtggcttc cgcactggac gcattctgcg cgcgcgtcga
     4981 ccagaccatc gccatccatt tggtttcccc gaccagcatc agcggcgtag aactgcgttc
     5041 cgccgtaacg ggcgtgggtt tcgttttgga agacgacggc gcgttccact ataccgacac
     5101 gtcgggctcg accatgttct ccatctgctc gctcaacaac gagccgttta ccaacgccct
     5161 tttggacaac cagtcctaca aaggcttcag tatgctgctc gacatcccgc actctccggc
     5221 aggcgaaaaa accttcgacg atttgtttat ggatttggcg gtacgcctgt ccggccagtt
     5281 gaacctgaat ctggtcaacg acaaaatgga agaagtttcg acccaatggc tcaaagacgt
     5341 gcgcacttat gtattggcgc gtcagtccga gatgctcaaa gtcggtatcg aaccgggcgg
     5401 caaaaccgca ttgcgcctgt tctcctaaaa tcgaatgcaa tcaaagcgga tatgtcgtta
     5461 cggcatatcc gctttttttc gggtaaaatt cagctgtttt caacctatca tttttcagac
     5521 gacctttgcc cgtttcatcc cgtatcagac aaaggtcgtc tgaaaacctt ctctcatgaa
     5581 tccaaactca aaacataaca ctaactttac caatcttctt aaaccgtctg atagtgatat
     5641 aaaacaattt gccgcacaac acatctgccg cctcaccgac ctcctcaacc gctacgccta
     5701 cgaatactac accctcgacg cgcccagcgt acccgatgcc gaatacgaca aattgttccg
     5761 cgaactcgaa gcgttggagt taaaccatcc cgagctcaaa ctgcccgaca gcccgaccca
     5821 gcgcgtcggc ggcgagcctt tggcgggatt tgccgaagta cgccacgaag tgccgatgct
     5881 gtcgctgacc aacgcttttt ccccgcaaga tgaaaacggc gtgttcgacc atgccgaaat
     5941 gtacgccttc gaccaacgcg tgcgcgacgg cttggacggc ggcaatcccg aatacgttat
     6001 cgaacccaaa ttcgacggcc tcgccatcag cctgctctac cgcgacggcg tattggtgca
     6061 ggcggcaacg cgcggcgacg gcacgacggg cgaagacgtt acccaaaaca tcaaaaccgt
     6121 atccaacatc cccttgcggc tgcacggcga aaatacgccc gaactcatcg aggtacgcgg
     6181 cgaagtgctg atgctcaaag ccgattttgt cgccctcaat aaaagacaag ccgaaaacgg
     6241 gcaaaaaccc tttgccaatc cgcgcaacgc cgccgccggc agcctacgcc aactcgattc
     6301 gcgcatcacc gcgcaacgca aactgcactt tttcccctac tccgtcgccc gccagcaaga
     6361 tggtttcgtc gcggaagaac acatccagga actcgcctat ttccaagcac tcggcttcag
     6421 tctgcccaat ggcaatttcg gctgtttcaa aaatatcgac gaagtattgg cgttttacga
     6481 acacatgcag caaaaacgcc ccgaactgcc ctacgaaatc gacggcatgg tcgtcaaagt
     6541 caacagcttg gcgcaacagc acgaactcgg cttcatctcc cgcgcgccgc gttgggcggt
     6601 tgcccacaaa ttccctgccg aagaagccct gaccattgtc gaagcgattg acgtgcaaat
     6661 cggcaggacg ggtgcggtaa cgccggttgc ccgattacag cccgtattcg tcggtggcgt
     6721 aactgttacc aacgccaccc tgcacaatca ggacgaagta tcgcgcaaag acgttcgcgt
     6781 cggcgatacc gtcgtcgtac gccgtgccgg agacgtgatt cccgaagttg tgcgcgtgat
     6841 ttttgaacgc cgcccgatgc gggaaaccgc cgttgccgtt tcagacggca tcgggcatcg
     6901 gcaggacgat ttgtttgccg aaacaccgtc cgccaatcaa acccaatccg ttccgctcca
     6961 caagccctac cgcctgccga cccactgccc catctgccgc agcgaaatcg aacgcgaaga
     7021 aggcgaagcc gtcgcccgat gcagcggcgg tatgctttgt caggcacaac gcgcgcaagg
     7081 cttaatccac ttcgcctcgc gcaaagcgat ggacatcgac ggcttgggtg aaaaacaaat
     7141 cgaacagctt gtcgcgcaag acctcgtccg ccacttcgcc gatctctacc gcctcgacat
     7201 cccgaccttg caaaaaatga aggaaacagc ggacaaaacc gttgccgaat cggatcaaat
     7261 gccgtctgaa ggcagttcgg tcggcgcgtc gggcaaacat aaaaaacaac cagtcaagtg
     7321 ggcggaaaac atcctcgcag gcatagaagc cagcaaaacg cccgaactcg cgcgcttcct
     7381 gttcgcgctc ggcatccgcc acgtcggcga acgcaccgcc aaaacgctgg cacaggcatt
     7441 cggcacattg gaacgcgtcc gccgcgcccc cgaacccgtc ctcgcctgcc tgcccgacat
     7501 cggtacggta gtggcgcgtt ccattgccca cttcttcgcc caagccgaac agcaggcaat
     7561 gatagacgaa ctgctcgccg caggcgttgc cccgcaaacc caagccgtta ccatcccgcc
     7621 cacccgccac gccgaaccgc aacgctggat cgcccgcctg cccggtttca aaatcagcga
     7681 aaacaaagcc caagccttat gggaactcgc cggcaaaaac atagaagggc tgcaaaccga
     7741 caaagccctc cccaccgact ggcaggcgtg gcgcagcgaa ccacaaaacg ccgccctgct
     7801 cgaaaacctg aaaaccttct tcgcgcaaat gccgtctgaa gacgaggcgg cgcaaggttc
     7861 agacggcatc aataaagccg tggcgggcaa aaccttcgtg ttaaccggca ccctgcccac
     7921 cctcaaacgc gaccaagccc aatccctaat cgaagccgca ggcggcaaag tttccggcag
     7981 cgtgtccaaa aaaaccgatt acgtcgtcgc cggagaagcc gcgggcagca agctggaaaa
     8041 agccaatgcc ttgggcgttt ccgtcctcag cgaagccgaa ctgcttaccc tgctcggctg
     8101 aacccatgcc gtctgaaccg ccttcagacg gcattgccca cttttccgaa ccacaccatg
     8161 cacaccattt cctttccaaa ccgcacccga ctcaccgccc tgccgcccct gtcgctttac
     8221 atccacatcc cgtggtgcat caaaaaatgc ccgtattgcg acttcaattc ccacagcctg
     8281 aaaaacggat tgcccgaagc cgcctatatc gacgcgctgc tgaccgattt gcagcttgaa
     8341 ttgcccaata tttggggcag gccggtggaa acgatctttt tcggcggcgg tacgcccagc
     8401 ctgtttcagg cggaatcgat tgaccgtttg ttaagcggcg tgcgttcgct gttgcgcttg
     8461 cagccggaag cggaaattac cttggaagcc aatccgggga catttgaaat cgagaagttt
     8521 caaggattta aagacgcggg tattacgcgg ctttctatcg gcgtacagag tttcaacgac
     8581 gatatgctct caaggctggg gcgcgtccac aacggtaagg aagccttaac cgctatcgat
     8641 accgccttga aattatttga taaagtcaat atcgacttga tgtatgccct gccgaaccaa
     8701 accgttcaga cggcattgga cgacgtacaa accgctatcg ctacgggcgc gacccacatc
     8761 agcgcgtatc atctgacgat ggaaccgaac acgccgttcg gtcatacgcc gccgaaaggt
     8821 ttgccgcaag acgaagccgc cctcgacatc gaagacgcgg tacacggcgc gctggaaggc
     8881 gcgggttttg tccactacga aacatcggct tttgcgaaac cagccatgca gtgccgccac
     8941 aatttgaact actggcagtt cggcgattat ttaggcatag gcgcgggcgc gcacggcaaa
     9001 atttcctatc ccgaccgcat cgagcgcacc gtccgccgcc gccaccccaa cgactacctc
     9061 gccttaatgc aaagccaacc gagtgaagcc gtcgaacgca aaaccgtcgc tgccgaagat
     9121 ttgccgttcg aattcatgat gaacgccctg cgcctgaccg acggcgtacc cgccgcgatg
     9181 ttgcaggagc gcacgggcgt accgagtgcc aaaatcatgg cgcaaatcga aacggcaagg
     9241 caaaaaggcc tgctggaaac cgaccctgcc gtattccgcc cgaccgaaaa aggacgcttg
     9301 tttttaaacg atttgctgca gtg
//
LOCUS       DAWISC010000069         9139 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWISC010000069 DAWISC010000000
VERSION     DAWISC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.069"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(175..867)
                     /locus_tag="WIW87_001165"
     CDS             complement(175..867)
                     /locus_tag="WIW87_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5174333.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WIW87_001166"
     CDS             complement(975..2168)
                     /locus_tag="WIW87_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ5174334.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIW87_001167"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIW87_001167"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ5174335.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WIW87_001168"
     CDS             complement(3021..3950)
                     /locus_tag="WIW87_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ5174336.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIW87_001169"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIW87_001169"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ5174337.1"
                     /translation="MRIEITPISESALVYRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WIW87_001170"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WIW87_001170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ5174338.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQNNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLIAGNHAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7509..8048
                     /locus_tag="WIW87_001171"
     CDS             7509..8048
                     /locus_tag="WIW87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ5174339.1"
                     /translation="MSQVFKDFDLSSVWKTNSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGAMGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8289..8450)
                     /locus_tag="WIW87_001172"
     CDS             complement(8289..8450)
                     /locus_tag="WIW87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174340.1"
                     /translation="MNIINYKGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPKLV"
     gene            complement(8458..8661)
                     /locus_tag="WIW87_001173"
     CDS             complement(8458..8661)
                     /locus_tag="WIW87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174341.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8574..8933)
                     /locus_tag="WIW87_001174"
     CDS             complement(8574..8933)
                     /locus_tag="WIW87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ5174342.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8986..9123
                     /locus_tag="WIW87_001175"
                     /pseudo
     CDS             8986..9123
                     /locus_tag="WIW87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2313 a         2494 c         2250 g         2082 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg tgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccagttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg tagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcacttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaagcggac aagccagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgttt gggcgcgatg attcccatca atgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg cgcccgatcc
     5281 gcacacacgc cgcatgattg agtgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa acaacgaaat
     5401 cctgcgccag cactaccaaa accgcttcaa ccacattctc gttgacgagt tccaagacac
     5461 caacaaactg caatatgctt ggctgaaact gattgccggc aaccacgcag cagtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgtgattgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatc cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcttgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca ttccctacaa aatctacggc ggcttgcgtt tttacgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaactg tccctgtccg aaatcatcgt cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgtctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgaattca aacccgaaga cagcaacttc gaaatcctgc ctgaaaacat
     6361 ttcagacgac cccgccttcc ccattctcgc cttcctaagc aatgccgccc tcgaatccgg
     6421 tgaaaaccag gcaggcgcag gcgaaaaggc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa tttaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgccaaa ccgccgcatc
     6781 caaagataaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggacaccaa
     6961 gtttgcgaaa ttggaagaga tgtaaatttg aaatgtaggt cggatattcg tatccgacct
     7021 acggcaaaaa ccttagcagg agagaataga aacccgtagc gtgggctttt tctatgaatc
     7081 aagcccaaaa tttcagacgg catttttagc cgtcattatc gtggatgaag cccacgctac
     7141 aatgtacaca cagagcaaat agagatgtgg gtcggatatt cgtatccgac aaaaacattt
     7201 gacgcgtcta ttgtttccga aacaccgctg ttggaaatgt cggatacaag aatctgactt
     7261 acggcaaaaa acgtagtaag gacaaagcaa aaggccgtct gaaaacggga agggcaattt
     7321 tgccgcaacc gccgccgtca ttcccgcgca ggcgggaatc cagacctttc ggcacggaaa
     7381 cttatcggat aaaaggtttc tttagattcc acgtcctaga ttcccgccgg aacataaatg
     7441 acggacggta aaagccgggt atgaataccc accctctgtt atcactgaga tcaataagga
     7501 agaacattat gtcccaagtt tttaaagatt ttgacttgtc ctccgtatgg aaaactaata
     7561 gttgggcaga tgaaaactac aaagaagccc cgtttacccc tgaaattttg gctgccgtag
     7621 aaagtgaact gggctataaa ttgccgcaaa gttttattga attgatggca gtacaaaacg
     7681 gcggaatatt tgtcaaaaac tgttttccga ccacgcagag aaattcgtgg gcggaaaatc
     7741 atgtgcaaat ttgcgaggta tcgggaatcg gttttgaaaa agaagggagt ttgtgcggcg
     7801 cgatggggca aaaactttgg ctggaagaat gggaataccc gcctatcggc gtgtattttg
     7861 ccaacgaccc gtcaggcggt catgccatgt ttgccttaga ctatcgggcg tgcggcaaag
     7921 acggcgagcc gaaagtggtg tttgtcgaac aagaatcgga ttttgaaatc gtcgaacttg
     7981 cccccgattt tgaaaccttt atccgcagct tgcggcatga agatgagttt attgacgaag
     8041 aaatataaaa cggtggttga aaaactgaaa tcatcaagag aaaacgggcg aaataacggg
     8101 taatcgcttg aatccgtaag gaaaacggtt tggtggaacg cgccatccaa gacctttgca
     8161 aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt ggagctatct
     8221 caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat taattgttgt
     8281 tctagctctt ataccaattt tggattgcga attcctgaca caatctcaaa ttcttctgca
     8341 tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg aagctcttct
     8401 cttgttaaag aaataataat gtcatcacct ttgtaattga ttatattcat aataatttta
     8461 tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc caccatcttt
     8521 tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg ctcttatgtg
     8581 tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga aagtgtcaaa
     8641 tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt aataatctta
     8701 tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt attacaatga
     8761 tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc ttgttctcct
     8821 tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc gggggtaaat
     8881 ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat cattagggct
     8941 gtcctagata aatagggaaa ttcaaattaa gttagaatta tccctatgag aaaaagtcgt
     9001 ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgcaggtgt aactgcaaga
     9061 acagcagcag agttagtagg cgttaataaa aataccgcag cctattattt tcatcgttta
     9121 cgatgactta atttatcaa
//
LOCUS       DAWISC010000070         9129 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWISC010000070 DAWISC010000000
VERSION     DAWISC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.070"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..377)
                     /locus_tag="WIW87_001176"
     CDS             complement(<1..377)
                     /locus_tag="WIW87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5174343.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPD"
     gene            complement(520..1041)
                     /locus_tag="WIW87_001177"
     CDS             complement(520..1041)
                     /locus_tag="WIW87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174344.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(1043..2215)
                     /locus_tag="WIW87_001178"
     CDS             complement(1043..2215)
                     /locus_tag="WIW87_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5174345.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAQREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDAVKHLLPDSVQALITVIGFNETLE
                     LVRLMGGTTYPLRQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALY
                     ELRNRKIRSQFDRQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            2261..2518
                     /locus_tag="WIW87_001179"
     CDS             2261..2518
                     /locus_tag="WIW87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ5174346.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2585..4570)
                     /locus_tag="WIW87_001180"
     CDS             complement(2585..4570)
                     /locus_tag="WIW87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5174347.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPKVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRT
                     AKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAP
                     KLAHSYRWFVQWAEAENMPVNDVPNLSMVRRVWEKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDAMRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRPGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPD
                     MLSAQELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAVWDELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4632..4874)
                     /locus_tag="WIW87_001181"
     CDS             complement(4632..4874)
                     /locus_tag="WIW87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ5174348.1"
                     /translation="MTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKALDAPYLK
                     GERIIAAAIGVPAEEIWPSRFEKRNHKPTFPRSINR"
     gene            5384..5824
                     /locus_tag="WIW87_001182"
     CDS             5384..5824
                     /locus_tag="WIW87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174349.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHEDSILINHAETDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDLSNAQDDIAIIGRVEWYGRSID"
     gene            6073..6624
                     /locus_tag="WIW87_001183"
     CDS             6073..6624
                     /locus_tag="WIW87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5174350.1"
                     /translation="MSGININLKNGDSLLIRGPSGCGKTSLLRALAGLWPFGSSGKVS
                     RPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYLVDKLDKTDD
                     WQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQKLPDSIIIS
                     IGHRSTLNAFHNMRLDVGNVACG"
     gene            6729..7397
                     /gene="hda"
                     /locus_tag="WIW87_001184"
     CDS             6729..7397
                     /gene="hda"
                     /locus_tag="WIW87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ5174351.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            7394..8062
                     /locus_tag="WIW87_001185"
     CDS             7394..8062
                     /locus_tag="WIW87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5174352.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            8418..8525
                     /locus_tag="WIW87_001186"
                     /pseudo
     CDS             8418..8525
                     /locus_tag="WIW87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2312 a         2520 c         1896 g         2401 t
ORIGIN      
        1 ttatccggct taaagccgtc ttcgccgcgc agtttccagc cggttttctt ggcaacggct
       61 ttgcccagca cctgacacca gcgtttattt ttcggcgtgg acaaggcttc gatgcctgtc
      121 gccgttttgt tcgccgccgc attggtgtgg aactcaatcg ccacatccga gccgcgaatc
      181 agcttgaccg catcgcgcaa gggcatattg cctttgcctt cgccgtcggt gcgtacggtc
      241 aggccgtaat cgttacgcag gattgaagcc acaatgttgc gcatatcctg cgccaagtcc
      301 gcctcacggt cgctgccgtt gactgcgccc gggtcggtgt tactgtgtcc ggcagtcaga
      361 caaataattt tgctcattaa agcctccctc aaaatcagat taaaatgcac tttcagaggc
      421 ttacattttc aaacggcatg gcttttgcag cgggcgaaac agtgtcagta ggcaacaaaa
      481 aagccgcctg ttgttcagac ggccttagaa tcgtttattt tattttttcg gcaaattggc
      541 ttcagcttcc gttattgttt tacggcattc ttgcattgat tttcggtaat tttgataact
      601 ttccttatct aaatcagttg taccgcctcc accgcttgtt accatactct tcatcaggat
      661 ttctgcatct tgcgcagcct gtttgcagac gcggtatggg tcatcatctc cattcgtgtc
      721 cgcataataa gcgactgctt gagtaatatt ttcacgatac tttgcaagat tttcttgatt
      781 aggtatttct ccttttaaaa acctctcaaa atccccgcca aggccttgtt gcaatacacg
      841 gaaacgggcg gcgcgttcaa aataaaaagc ctgtttctca tctgcaaatt gatatttatt
      901 ggcaaaaaaa tactgctccg catcagcttt ctgcgattgt tcttgcccac acgctgccaa
      961 acccaacgcc agcagcacaa aataaaactt tttcatattt ttctccaaaa taaaatgcca
     1021 tacatctgca tggcattcca ttctaaaaca aattctcctg ctccgcttcc ttatccgcct
     1081 gctttaaaat tcgccacaca tggcggtcgc ttaggcggtg tgccaaggcc aaatcgttaa
     1141 cggcctcata agcaggggta ccgcctgccg tctgccggtc aaactgactg cggattttac
     1201 ggtttcgcaa ctcatacaag gccgtctcgc aacggggtat aaacagattg cacggagcca
     1261 ttgcctccac cagccgaccg gccgcctcac tgccgataat ctcttccaag tatgcaacac
     1321 gggattgact gtttttcgta taaccctgcc gcaaaggata agtcgtaccg cccatcaggc
     1381 gcaccagctc cagcgtttca ttaaacccga tgaccgtaat caacgcttgt acactatcag
     1441 ggagcagatg cttgaccgca tcaggcaaag ccgctttcgc aatctcaaag agttcgtcat
     1501 ccggcaaaga ttcgcccaaa ttcagcgcaa cagacacgcg gctgtaaagc tgtaccagtt
     1561 cgccatgctt accgcgcagg ttggccacca gtcggggcat accgctcaac accaagccgc
     1621 agccagtctc gtcgtgcaga cggcgtacaa tttcaagggc gcgtaaaggc aggttttccg
     1681 cttcatcgac cacaatcaga cggcccgaat cgcgcaggcg gtcagataca gactcaaaca
     1741 aatcattcag gctgcccatc gccgatacct tcgccgcagt cgccaacttg cgcatcaaga
     1801 ccaaagccgt aaagctcgga ttagcctcaa tcaagatggc cgcagggttt ttctcgcagt
     1861 agtttttgac cgcctgagtc ttgcccaatc ccgcctgacc gtagatcacc actgtttcgc
     1921 cgccttcgtg cgcatcgcgc atcacttctg caatacggcg ggtcgtctta gtcgatacaa
     1981 aacccaacac cagctcttcg cgttgcgctt tactgtcctg catctccaaa aacgcctcga
     2041 ttttcggctc gatggtttca taattgccgc ctttttccgc ataagtgccg ttcagataca
     2101 tactgatgga tgccggcgaa gtaccgatac cgcgtgccag ttgggtttgg ttcatgcctg
     2161 atttttcttt aaattcaacc agtttttgtt gcaatgcttg attaatttgt ttcattttta
     2221 atatccttga gttttaaaca acctttaaag gccgtctgaa atgaaagagt ttcccgattt
     2281 agaaaaacgc ctgatcatgc tggaagtcgc cgttcaagac ttggaagaca ggtcacttgc
     2341 cgattctttc gtactttcct ggctgctcca gcggattacc cgtcaagagc cgacttcgat
     2401 tgagcaggtt cgcagtttcc ttcaagcgca ggcaaaaacg tttgagcctg attccgttca
     2461 gcgggaacac cttgaatctt tgcttgagct cgttgaatcc gcccaagagc tcgcttgagt
     2521 ttcaattttt caacaagctc ggccgaaaac gctgcatttt gctgtttgtc catcatgttt
     2581 tcctttatct atccgcctca aacaacacaa aatcgtctgt gcccgttttc ggcaataccg
     2641 catactccgc ctcgatgtcg tttccgccca aatgtcccag ctcgtcccaa accgcagcct
     2701 gttccagagc aggattgact tccgcgtttg cgagcttgat tgcattttcc gcccgcttta
     2761 ttttgccttt tcgacgtttt tccgccagtt ggtcgatacg agccgtcggg aaagcctcgc
     2821 gggtattgcc gttggcctgc gccttagtaa taaacttgcc gtccatatca aacacattga
     2881 ccgccgacgc atcgctcaaa tcgtagctga cccgtacctc gtctttgtga tactccgcca
     2941 gctcggttga gaaataagag ttgttaaaca aatccagcca accgcgctgt acctttcgca
     3001 cctcttgcgg cataaacatc gtcgccagct cttgcgccga caacatatcc ggcgcgatac
     3061 cgtcctgttc cagcctcatt tcccgataag ccttaggcgt ataatgcccg ccgtcaggat
     3121 gtcggggcag ctcgccgtgc gggcggttgt tgtattcgtc gatacacttg accacatccg
     3181 ctataaaacg cgaccagctc ggcagttttt tcaaatattt ctgttgttcc tccgtcaaat
     3241 ccttgccttt ttccaaagcg ttaaacgcac tttccatctt gcggtacatc aggttcttcg
     3301 tgctgctgtc catccctgca cccgcaaacg tctcatactg gcgcgccatc tcaatcagat
     3361 tgtctttcca ccatcgctca atgatgccgc gcccttgcgg gttgcccgcg atacccgttt
     3421 catggcggat acccggtcgg gacgtaatac ccgtgatttc atggtctatc gtcttgccgg
     3481 tttggccgcc gccgttatcc gagtaataga taatcggcaa accaaaatgc ttgaccccga
     3541 tacgcatagc gtccgatacc gccacacaac tttcagcaag agagaccgaa aacccgacca
     3601 caaaccgcgt acaaccatca ataatcaccg tcacttccgg cttaaatggt ctgccatgta
     3661 ccggatgcgc cacctttgcc ttaaagctat ggccgtcgcc gatccaaaca tcgttcggct
     3721 tcaaagcccc ccaatcacgt ttcacataag gcagcagcga tttataagcc gcccccgttt
     3781 tcctgccgcg ctcctgcata atcaacggga gcttttccca aacgcgccgc accatactca
     3841 agttaggcac atcattgacc ggcatatttt ccgcttccgc ccactgcaca aaccagcggt
     3901 agctgtgcgc cagctttggc gcggacggaa tattgtgaaa ctgcataaac atcggcaacc
     3961 atccgtaact ttcaatcggc ttgaccgcct tcgtcgtctt cggagccaaa gcaaccaacc
     4021 gctccgtcgc gttttccgct ttcaaataag cagaaatcca gccgtctaaa gtacgttcgc
     4081 cgaccttcgc cgtccggctg cggtcattgg ccttttccaa gttcccaagc gtgaccgcgt
     4141 ccaatttacc ttctgccagc aagcccaaaa actgagccac cgcagccttg gcagagcaat
     4201 cgtattcgta tttaatcccc aataccgccg ctaccaccgc acatcgcgca tccgccaccg
     4261 accgttgttt ctcgttcaac agcttggccg cttcagccag tgcttgagcc gacatcgccg
     4321 tccccggtct gactttgggc agcattttcg gcatcttctc cgccagctcg tccgactgcc
     4381 gtttcatgat ggctgctcgg atttcggcag ggagggaagc gatttcatac agttttttag
     4441 gacggcctct tcctttttgc tcaaaacagt gttgccaatt atttttcttg gcatggtatt
     4501 caatcccttg tctatcagtt ggtaggctcg gaattcctaa tttcgcaata tcagatgcag
     4561 atattttcat tttttccgct ttctattttt gcttaaatgc ggtactcttt tggaactatt
     4621 tagcaaaaca gttatctatt tatagatctt gggaaggttg gcttatggtt tcgtttctca
     4681 aaacgagatg gccagatttc ttctgcgggt actccaatcg ctgctgcaat gattctttcg
     4741 cctttcagat aaggagcatc taaagctttc cctaatgtat ttggtgctaa attagcctcg
     4801 atagataacg ctctcacaga ccagcctgcc tttttaagac gagccacaat gtcagcacga
     4861 tgccaatcag tcatgctttc ttgctttttt tcattctcgc aattccttaa ttaagtaggt
     4921 ttattaaccg ccgtcattta gcaaatgagt gaattacatt tagcaaaaca gcttcatgaa
     4981 actcttttgc taaaatattt tctcaaaaac gtaattattt ttacaaaata ttgattattt
     5041 gagtaatctt atttaagcaa aagagtttct ttttttgctt aatgttttgc ttaaatgagt
     5101 taaattagga ttactatgga tactttttta gataggctca aatttctttg gcctcatggg
     5161 gctaagccat ctgacatcta taacaaaata gaaatgtcgg cttctggatt taatagagtt
     5221 tggaaagagg gagctatccc aacagccgat tacctagtaa aaattcagtt gtgatttaaa
     5281 ctggctgctg accggaaaag gcttgccgta tctcgataaa gccaggccgg aaaacgccgg
     5341 agcattctct gtaagtagaa attctgatgg aatcaccgac actatgggca atcctgtcga
     5401 cctgtccgag tttgtattcg tgccgcgcta caacgtagcc gcagcagcag ggtatggcgc
     5461 accagtcttc ggcgaagaac ctttattctg cttggctttc cggaaatatt ggattgacaa
     5521 ctatgtgacc aaacagccgg aaaaactgtc tgtaattacc gttaaaggcg attcaatgga
     5581 gggcgtgctc aatcatgagg attccattct catcaaccac gccgaaactg acccgcgtga
     5641 cggcctatat gtcttgcgta taggcaatga cctctttgtc aaacgtgtgc aacgtatacc
     5701 gggcaaactg cttgtaacgt ctgaaaatcc acgttacgca ccgtttgaaa tagatttgag
     5761 caatgcccaa gacgacatcg ccatcatagg ccgtgttgaa tggtatggcc ggagtattga
     5821 ttaaatccat tttaaaaacc tcttaaaaca gttttaaaaa ttccctaatc tcgtcagctt
     5881 taaacagaaa accgcgcatt ccggcgcggt tttgtgaaaa agctggcgta acttttccgg
     5941 atacaaaaaa acgccgaaat ccacgtcttc cgaagatttc agcgtttttt tactctattt
     6001 gttccttgtg caaaaactaa cagtccccca caggaaaacg tcgctctgtt ccgccacaac
     6061 ggcgaaatcc tgttgagcgg catcaacata aacctcaaaa acggcgattc cctgctgata
     6121 cgcggcccga gcggttgcgg caaaacctcg ctgttgcgcg cgctggcggg gctttggccg
     6181 ttcggcagca gcggcaaagt cagccgtccg ccgcatcaag acatcctctt cctgccgcaa
     6241 cgcccgtaca cggcacaggg cagcctgcgc gacgcggttt gttaccccga cattgacaag
     6301 cagcatcccg agttagccga agccatgaac acctgccgct tgggttatct ggttgataaa
     6361 ttagataaaa ccgacgactg gcaacacaaa ctctccccgg gcgaactgca acgcgtcgcc
     6421 ttcgtccgcg ccctgctttc caagcccaaa atcgtcctgc tcgacgaagc caccgccgct
     6481 ttggacgaac cgaccgaggc cctgctctac cgcgcactga aacaaaaact gcccgacagc
     6541 atcatcatca gcatcggcca ccgcagcacg ctcaacgcgt tccacaatat gcgcctcgat
     6601 gtcggcaacg ttgcctgcgg ttgaacgatg ccgtctgaag ccgttttcag acggcatcgt
     6661 tcaaattgga aaggcgcgcg aaaacaacgt acaataaacc cgtttaccac ccctcggaat
     6721 gccgaaccgt gaaccagctt attttcgact ttgccgcaca cgactatccg agtttcgaca
     6781 aattcctcgg cacggaaaac gcggaactgg tctatgtcct ccgacacaaa cacggacagt
     6841 ttatctatgt ctggggtgaa gaaggcgcgg gcaaaagcca tcttttgcag gcgtgggtcg
     6901 cacaggcgct cgaagccggc aaaaacgccg cctatatcga tgccgcctcc atgccgctga
     6961 ccgatgccgc gttcgaggcg gaatacctcg ccgtcgatca agtcgaaaaa ctgggcaacg
     7021 aagaacaagc cctgcttttt tccatcttca accgcttccg caacagcggc aaaggctttt
     7081 tgctgctcgg ttcggaatac acgccccagc agcttgtcat ccgcgaagat ttgcggacgc
     7141 gtatggcgta ctgcctcgtt tacgaagtca aacccttaac cgaccaagaa aaaatcgacg
     7201 cgctcgccag tatggcggcg gcacgccaag taaccgtcga ttccgaaatt ttcgaatacc
     7261 tgctcaaaca ctggcggcgc gatatggaca gcctgatgat gatgctcgac acgctggaca
     7321 actacgccgt cacgatgggc aaacgcatca ccctgccgct tttgcgccag cttttgaaac
     7381 aacaggaaac ccaatgaaaa acctcgccat cttcgacctc gacaacaccc tcatcaacac
     7441 cgattccgac cacgcctggc cgcaatacct catcaaaaaa ggcttggttg acgctgccga
     7501 aaccgaagcg caaaacgaaa aattctaccg cgactaccaa aacggctgcc tcgacatcga
     7561 cgcattcctc aaattccacc ttgccccgct cgctcgttac agcaaagaag agctggcaga
     7621 gtttcaccgc gaatttatgg cggaatacat catcccccac atctcgccta tgcagcgtat
     7681 gctggtgcag agccaccaaa tggcaggcga cgaaaccctt gtgatttcct caaccaacga
     7741 gttcatcatc acccccgtct gccacctttt cggcatcacc aacatcatcg gcacacaact
     7801 cgaaaccggt tctgacggcc gctacaccgg caattacatc ggcacgccca gcctcaaaga
     7861 aggcaaaatc acccgcctga accaatggct tgccgaacgc ggcgaaacgc tccaaagcta
     7921 cggcaaaacc tatttttaca gcgactccaa aaacgacctg ccgctgctgc gccttgtcag
     7981 cgaacccgtc gccgtcaacc ccgatgccga gctggaaaaa gaagccaaag aaaaaggctg
     8041 gccggttttg aatttcaaat gacgcaatgc cgtctgaagc ccgattcggc gttcagacgg
     8101 cattgaggct gaaatccaaa aaactcaaag ccacaggaat ttatccggaa aaacaaaaaa
     8161 cacaggaatt catcgggaaa aacaacaatc tttccaccgt cattcccacg aaagtgggaa
     8221 tcttgatccg tccgcacgga aacttatatt tcgtcatttg cacccgacat aattcaatac
     8281 tttattttgc aataaattaa cggatgctga caaattagat tggattaatt accctattat
     8341 ccatattggg ttatcgggtt gctaattgag cagagcgtag caaaccataa agtgagtgtt
     8401 tccacccact ctgcatttat agtggattaa atttaaatca ggacaaggcg acaaagccgc
     8461 agacagtaca gatagtacgg caaggcgagg caacgctgta ctggtttaaa tttaatccac
     8521 tatactatga agtggttatt aaatatgata atgagaccta ttaaatttag tatggtaaat
     8581 acgttattat ttattgttat atgtagttca ttttttgatc tgctcgttca attatgtaca
     8641 attttatttc atagccaaaa aatatacttt attacattat ttttattatt tatttttaat
     8701 tttgttacaa aatctatcta tatggcaatt atttatccta ttttatattt ttttacgata
     8761 aaaaaatatt atccttactc taggaaagtg ataattctat tatcattagc attatctata
     8821 tattttagtt ttatggactt ttactttttt tccatatatt cagataacct tagctatgaa
     8881 acggagcctt tacatttata catccctatt attattaatt ttttctcact tttagtttct
     8941 aattttattt tatcttttat caacaagtaa atgtatatac aaaatacaat ccaaaagatc
     9001 ctaagcaaac caccaatccc acaccaccct taccggttca aatccgcaca aaaacccaca
     9061 tcccgtcatt cccacgaaag tgggaatcta ggacgtatag tggattaaca aaaatcagga
     9121 caaggcgac
//
LOCUS       DAWISC010000071         9106 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWISC010000071 DAWISC010000000
VERSION     DAWISC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.071"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(44..3088)
                     /locus_tag="WIW87_001187"
     CDS             complement(44..3088)
                     /locus_tag="WIW87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ5174353.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFHRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESQTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMTAIDIQPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLLPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3253..3933
                     /locus_tag="WIW87_001188"
     CDS             3253..3933
                     /locus_tag="WIW87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174354.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMMGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALTVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3962..4321
                     /locus_tag="WIW87_001189"
     CDS             3962..4321
                     /locus_tag="WIW87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ5174355.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASIPPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4426..4656)
                     /locus_tag="WIW87_001190"
     CDS             complement(4426..4656)
                     /locus_tag="WIW87_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174356.1"
                     /translation="MNIFVTNFCYWFISLLAISYIPSINAWHDELIDDINFGKRVMMV
                     TFFAFLGTVIERFFKKKPWWFYPAKAFSLLQT"
     gene            complement(4835..5371)
                     /locus_tag="WIW87_001191"
     CDS             complement(4835..5371)
                     /locus_tag="WIW87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ5174357.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WIW87_001192"
     CDS             complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WIW87_001192"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ5174358.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5817..6419
                     /gene="plsY"
                     /locus_tag="WIW87_001193"
     CDS             5817..6419
                     /gene="plsY"
                     /locus_tag="WIW87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ5174359.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            6496..7341
                     /locus_tag="WIW87_001194"
     CDS             6496..7341
                     /locus_tag="WIW87_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ5174360.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGSRYLL
                     DSKIR"
     gene            7587..8561
                     /locus_tag="WIW87_001195"
     CDS             7587..8561
                     /locus_tag="WIW87_001195"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ5174361.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANSAVAGLASEEEDTFVNAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(8704..8922)
                     /locus_tag="WIW87_001196"
     CDS             complement(8704..8922)
                     /locus_tag="WIW87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ5174362.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2096 a         2701 c         2111 g         2198 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggcagtaccc ggcagcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac ggattgccga ttttttctcc ccttgctgcg gcttcggcga
      781 ttttttgatt gaagcccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggtgcttttc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatgcagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaaat ctttttccag attcagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaattac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggaggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttcaga tgggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacattttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ttcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggtct cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggctgaa
     1861 tatcgattgc ggtcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtctga ctttccggaa tcaggcgaga aattcgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg
     2161 ccgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctttcccctg ccctgcatga cggtggaacg gcgcattccg tgcattttcg gctttgactg
     2281 cagaaaaggc agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact
     2341 cggaaaacgc ttctttgtcc gcatcgaaca tatgggaata cgtgcgttga agcacagggt
     2401 cgtccaaacc gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga
     2461 ccgctgtttc gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg
     2521 cgctgtttaa agcacgcgtt gcttcttcca aagtgatttc tttaaaaacc ggacggacgg
     2581 gcgaaatttc gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc
     2641 ggcggttgtc gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag
     2701 cttctgcttc ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg
     2761 atgcggtcgg aacatccgca cggtttgcag aagcaggcgg aacgggcctg tccggttcgg
     2821 attcgtgcgc cagatagttt cggcagaaac ggacgacacc gacaaacagc caatacactg
     2881 ccgttttgac ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt
     2941 tgatttcagg cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc
     3001 gcatccactc gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca
     3061 aaataacgat caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg
     3121 ggtttatttt aacgtgtttc catcccaaaa aaaacaaccg gccggcaatt ctgccgaaac
     3181 cgattcggct ttcggtttgc ccccgccgac agtataatag ccgtcccttc ccttaatctt
     3241 tgcggcattg ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg
     3301 tggattgtcc ttatcctgat actggcagct tccgcgccgt cggcattccg ctcgcttgcc
     3361 cgctaccgtt ccgcccttcc cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac
     3421 gcctctgccg gcggcggaca gcttgcccag atgaactacc acctgctcgc cgtcaatttg
     3481 gttgcactga tgatgggcac ttccgccgcc ctttggcttg ccgcgctgct gatgctgccc
     3541 tactgcctgc tgtttgccgg ttctgtcggc gcatatccgc cgaacgcttt ggtgctgata
     3601 ctgcccgcgc tgaccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat
     3661 attttcatct ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc
     3721 gggctggtgc tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg
     3781 acgaccgccc tgcccgtctt tattctgctg gcgtgggcgg aagccttcct cagcggcatt
     3841 tcaactgcca tatttgttgc actgaagccc cattggatta acactttcga cgacaaccgc
     3901 tatcttaagt ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac
     3961 catgctttcc aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg
     4021 gctgctcaac ctcgccgttc ccgcatcaat acctcccgcc acaggcaacc tgtttgccaa
     4081 ctggataggc gcttttctta tcggaatctt tgccgaaacc gtcaaccacc cgcaatggaa
     4141 gctgctgctg attaccggtt ttctcggcag cctgaccacg ctttccggat tttcgctgga
     4201 aaccgtaacc ctgctccaat tgaaccgtcc cgcttcagca cttgccaata ttttcctgca
     4261 cacggcaggt tcgctgctgc tgacctggct cgggctgaag ataggggcgg cagtcaaata
     4321 accgcaacaa attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta
     4381 aatactggct gtgccaatta ccgttctcaa attcaaattg agcttttagg tctgtaacaa
     4441 agaaaaagcc ttggcaggat aaaaccacca aggctttttc ttaaaaaaac gctctattac
     4501 cgtgcctaaa aatgcaaaaa aagtaaccat cataaccctt ttgccaaaat taatatcatc
     4561 tattaattca tcatgccatg cattaataga tggtatataa gaaatagcca ataaactaat
     4621 aaaccaataa caaaaattag taacaaagat attcattatt aaccatttta aaaattatca
     4681 cgttctttta ggtaattatc atcccctttt ctctcttatt actaaagcac gaatgacttt
     4741 aaaggacgcg cccagataca agccgaaaag gcatatccca tccgccaaac aaaccgcccg
     4801 ccgatccaat ccggcgggca agtccaacat cctgtcaatc cttcatcaac cagtattgca
     4861 aaccgattaa tgtcttgccg tctttaattt catcgtttgc caatgcctga cggacttctt
     4921 ctttcgacat caatacggtt tccgtaatct cgtcttcgtc attggcaagc gtactgccca
     4981 aacgcacgcc ttccgcttcg aacagataca ttttttcgtt gcaaaaaccg accgccgtat
     5041 aaaaactgta aagcaggcgt acgctgtcgg cggtataagg tgtttcctcc gccaattctc
     5101 gcagcgcaca cgctgccata tcctcgcccg ccacatccag cttgcccgca ggaagttcca
     5161 atgtcgcctg atttgccgca taacgccact gccgcaccaa aaccactttc ccctcgtccg
     5221 taaccgccaa cacgcatgcc gcacccggat ggcggatgac gatacgctgc ccttcattgc
     5281 cgttgggcaa cctgacctta tccctgctga tactgacgaa accgccctcg taaatggttt
     5341 cgccgcctaa ttttacttcc ctcaaatcca cttcatatct cctttccgtt tcctattctg
     5401 ccgttcaatc ttcacgcttg ccgcgctcga tttccacgcc aacctcgcgc acgccttcca
     5461 aaatacccgg cttgacgatt ttcacgcgca cccacaccgc gccgaaatat cccaaaacca
     5521 aatcggcaat atattccgcc aacgcttcca aaagcaggaa atcctgttct ttcagatgtc
     5581 ggcgcagcgt ttcgcatacc tcggcataat gcaccgtatt ggcaatatcg tcgtccgaac
     5641 ccgctttctc gggaacaccg atgtccaaat cgacaatcag ggtctgcaac cgttcgcgtt
     5701 cccagccgta cacgccgata agcgtatctg ccttcatgcc gtgcaaaaag attttatcca
     5761 taatccgccc cgttttttgc taaaataacc gcaccattct aagtaaagta ccgcaaatgt
     5821 tcaacatacc ggctgttgct gtttcctatc tgatcggctc actttctttc gccgtcatcg
     5881 tgtccaagta ttacggcatg gacgacccgc gcacctacgg atcgggcaat ccgggggcaa
     5941 ccaatgtttt acgcagcggc aaaaaaaagg cggccgcgct gacgctcttg ggcgatgccg
     6001 ccaaaggttt agttgccgtt ttgcttgcac gcgtgcttca agaaccgctc ggtttatccg
     6061 acagcgcaat cgcggccgtc gcactcgccg cgctggtcgg gcatatgtgg ccggtgtttt
     6121 tcggatttaa aggcggcaaa ggcgtggcaa cggcattggg cgtgcttctg gcactctctc
     6181 ccgcaactgc cttggtctgc gcgttgattt ggcttgttat ggcattcggc ttcaaggtgt
     6241 cctcccttgc cgcattaacc gccacaatcg ccgcaccggt cgccgcatcc ttctttatgc
     6301 cgcacgtctc gtgggtttgg gcgaccgtcg ccattgcttt gctggtgttg ttccgccaca
     6361 aaagtaatat cgtcaagctg ctcgaaggca gagaaagcaa aatcggcggc agccgctgat
     6421 ttcccatcca tccctgttta tgccgtctga accccgcttc agacggcatc cgaatatccc
     6481 gccgttttac cgactatgct cagttacaga cacgcattcc acgccggcaa ccacgccgat
     6541 atgctcaaac atttcacctt gtttttagtg ctgcaatatt tcaaccgcaa agacaagccc
     6601 tactggtaca tcgacacgca cggcggcgcg ggtgtgtaca atctcgaagg cagcgaggcg
     6661 cagaaagtcg gcgaataccg acaaggcatc gccctgctcc gacaggcgca aaacctgcct
     6721 gccgaactct ctgactttgc cgcgcacata caaaaaatcc tgccctcgcc caaactttac
     6781 tgcggttctc cgtggctggc gcaatcgctg acccgcgtcg gcgacaaatt acgcctgttt
     6841 gagctgcacc ccaccgactt tgtccatctg caaaacaata tgggcgaagc aggattgggc
     6901 aaacgcggac aagtattacg cgaagacggt tacaaagggc tgatttccct gctgccgccc
     6961 ccgccgcgcc gcgccaccgt cctcatcgac ccgccctatg aggaaaaaca ggactaccgg
     7021 cgtgtaacgg aaacgctgaa agccgcttta aaacggtttg agtccggctg ttatctcatt
     7081 tggtatccct gcctcagccg cgaagaaagc cgcaagctgc ccgaagaatt gaaaaaactc
     7141 ctgcccgaca actaccttca cgccgaactt cacgtccacg cgccgaaagc cgacggtttc
     7201 ggtatgcacg gcagcggaat gttcgtcatc aatccgccct atctgttggc ggaacaattg
     7261 gcggcaaacc tccccgccct gacgcggctg ctggcgcaag acgaaggctc gcgctacctt
     7321 ttggacagca aaatccgctg accccgatgc cgtctgaaac ccttcagaca acatctcccg
     7381 tcaaataaac aaaaccggac gatataccgg cagtcggcac ttttaagaat aaatatcccc
     7441 tcatccgcct gccgcgttcc gacttgcccc atgccgtttt tttacactac aatcccctag
     7501 actatcctgc aaaacgcacc aaaggaatac gccacaataa cctgccgcct ttcagacggc
     7561 acaaaccttt ccgaaacgag caaactatgg acacactgac ccgattcctc cccgaacacc
     7621 tgcaacaaaa ccagctgccc gaagcactcg gcggcgtttt actgtccgtt gtttccgcct
     7681 gcaccgaaat caacgccaaa gtccgcctcg gcgcactggc cggcgtattg ggtatggcgg
     7741 gcacgggcaa tattcagggc gaagaccaaa aaaaactgga tgttatcgcc aacaacatca
     7801 tgattgacac actcaaagcc aattctgccg ttgccgggct ggcgagcgag gaagaagaca
     7861 ctttcgtaaa tgccggtgaa aacggacgct atctcgtcct attcgaccct ttagacggat
     7921 cgtccaatat tgatgtcaac atttccgtcg gtacgatttt ctccatcctt gaaaaacccg
     7981 aaggcgcatt ggcaaccgaa tcattccttc aaacgggcag acagcagctt gccgctggtt
     8041 acgttcttta cgggcctcaa acccagctcg tattcacatt cggacatggc gtatatatgt
     8101 tcacgctcaa tgccgaaaac gaatttgtgc tgaccaaaga aaacccgaaa gtacccgaaa
     8161 gtaccaaaga atttgccatc aatatgtcca accgccgcca ctggctgccc cccgtccaac
     8221 aatacatcga cgagctcttg gcgggcgaaa ccggtacgcg cggcaaaaac tacaatatgc
     8281 gctgggtagc cagtatggtt gccgaaatcc accgcatcct gatgcgcggc ggcgtgttca
     8341 tgtatccgca agacaaacgc gaccccgcta aacccggcaa actgcgcctg atgtacgaag
     8401 ccaaccctat gagcctgata ctcgaacagg cgggcgcatc ggcaagcaac gcatatcaag
     8461 ccatgctcga catacagccc gaaaacctgc accaacgcgt cgccgtcttc atgggcagca
     8521 gcgaagaagt cgcctatctc gaccgcctgc acgcaaaata gacagacagc cgttcacaca
     8581 agtacacggc ggcggcaatc cgataccgcc gccgttattt caaacagcac aagccttccg
     8641 gtcaaatgac aaaaatgccg tctgaacaca tttcagacgg catttcaaaa actcaaaccc
     8701 gaattatttt cccgctgctt tttgcaggat ggcgtacaac tcgtctttca gtttcaattt
     8761 ggcttttttc agctcatcga tggtttccgc accgctggta accggattgt tgaccagacc
     8821 ggtaatttta tcgtccagct cgttgtgttc gtcgaacaga cgggcgaagc gggaattttc
     8881 ctgtttcaat ttggaaatca aatcacggta ttctgggaac atatcgcttt cctctcttgg
     8941 tttacggtta aaaagtcaaa ttgaatcttt acattaatta taacaaagat aatattttta
     9001 tccagtgttt tatcggatac ggcaaaattt tcttttaaca ccgccgtccg gttgataaaa
     9061 acgttgacaa tatcacgctt tacgcataaa ttttcagcct ttccta
//
LOCUS       DAWISC010000072         8815 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWISC010000072 DAWISC010000000
VERSION     DAWISC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.072"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            179..1354
                     /locus_tag="WIW87_001197"
     CDS             179..1354
                     /locus_tag="WIW87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ5174363.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WIW87_001198"
     CDS             complement(1565..2113)
                     /locus_tag="WIW87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ5174364.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WIW87_001199"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WIW87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ5174365.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WIW87_001200"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WIW87_001200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ5174366.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6268..7656
                     /gene="fumC"
                     /locus_tag="WIW87_001201"
     CDS             6268..7656
                     /gene="fumC"
                     /locus_tag="WIW87_001201"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ5174367.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLSGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIHLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7721..8644)
                     /locus_tag="WIW87_001202"
     CDS             complement(7721..8644)
                     /locus_tag="WIW87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5174368.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALIVVGGAVTAAVGDRLFKRR"
BASE COUNT         2227 a         2519 c         2157 g         1912 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg catatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatc agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ccaatgtaaa cggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgca
     4861 aaaccttaat cggcaaaggc agtgccaaca aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggcgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccccgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaacaaggc gcgaaactgg
     5041 aagccgactg gaacgaactg ttcgcgcaat atcaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaagctgc cggacaattt cgatgaatac gttcaagccg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccatcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagttgcctg aattggtagg cggttctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tatgaacggt ttggtattgc
     5401 acggcggcgt aaaacccttc ggcgcgactt tcctgatgtt cagcgaatac gagcgcaatg
     5461 ccctgcgtat ggctgcgttg atgaaaatca accctgtatt tgtgtttacc cacgattcca
     5521 tcggtttggg cgaagacggc ccgacccatc aaccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtggcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgag caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggctcagaa gtcgagctgg
     5821 ctttggaagc gcaaaaagcc ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagacg ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg catcgccgta gaagccggac acgccgacgg ctggtacaaa tatgtcggac
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatttactct
     6061 tcaaagcatt cggctttacc gtggacaatg tggttgatac ggtgaaatcc gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttttatatt
     6181 ctcgcggcca tgatgctttc tcatcccacc aatctccatt ataatatttg cgaatcactc
     6241 ttattcacat ttcaaaagga gaaacgcatg agcacccgta ccgaacacga cacgatgggc
     6301 aatgtcgaag tcccatccga agcctattgg ggcgcgcaga cccagcgcag ccgcaacaat
     6361 ttcaaaatcg gtggcgaaac cctgccgcag ccgttgattt atgctttggc attggtgaaa
     6421 aaagccgccg ctgccaccaa tgtttccctc ggtaggatta agcctgaaca ggcggatttg
     6481 attacgcagg cggcggatga tgtgttgagc ggcaagctcg acgggcagtt cccattggta
     6541 gtgtggcaga ccggttccgg cacgcagtcc aatatgaaca tgaacgaagt gctggcaaac
     6601 cgcgccaacg aaatcgccgg tacgggtttg gcggcttatc agcccgtcca tcccaacgac
     6661 catgtgaacc acgcgcaatc gaccaacgac gcattcccga ccgctatcca cgttgccgcc
     6721 gcgattgaaa tcaaccgcca cctcatcccc gccgtaaaag ccctgcgcga cacgttggac
     6781 aaaaaagccc aagctttcgc ccctatcgtc aaaatcggcc gcacccactt gcaagacgcg
     6841 acgccgctga ctttgggaca ggaattttcc ggctacgttt cccagcttga tcacggttta
     6901 ggccgtctga acgatgcgct taaagacttg tatgaacttg ctttgggcgg tacggcggtc
     6961 ggcacgggtt tgaacagcca tcccgaatac gccgaaaaag ccgccgccaa actcgccgaa
     7021 ttgtccggct tgccgtttgt cagcgcgccg aacaaatttg aagccctggg cggacgcgat
     7081 gccgccgttg ccgcttcggg cgcattgaaa acgctggcgg caagcctgaa caaaattgcc
     7141 aacgacatcc gttggctggc aagcggcccg cgttgcggtt tgggcgaaat caaaatcccc
     7201 gaaaacgagc cgggttcgtc cattatgccg ggcaaagtca acccgaccca atgcgaagca
     7261 atgacgatgg tgtgctgcca agtgttcggc aacgacgtta ccatcggtat ggcgggcgcg
     7321 tcgggcaatt tcgagctgaa cgtctatatg cccgttatcg cctacaacct cttgcaatcc
     7381 atccacctgt tgggcgacgc gtgcaacagc ttcaacgaac actgcgccat cggcatcgaa
     7441 cccgtgccgg aaaaaatcga ctatttcctg caccattccc tgatgctggt taccgcatta
     7501 aaccgtaaaa tcggttacga aaacgccgcc aaagtcgcca aaaccgccta caaaaacaac
     7561 aaatcgttgc gcgaaaccgc cgttgagttg ggcttgctga cgggcgaaga atttgacgaa
     7621 ctggtcgttc ctgccgatat ggttcatccg cgctaatcct tccctcaaat aaaatgccgt
     7681 ctgaaacctc gttcggacgg cattttccgt tgcctgcaaa ctagcggcgt ttgaacagcc
     7741 tgtcccccac cgccgccgta accgcacccc cgaccacgat cagtgcgcct gcataaccca
     7801 aaccgttcat atccggcgcg gcaaaagtat caggcatcac ataatgcccg agcaaagaaa
     7861 atattacggt aaacacgggg agcaaggttg ttaccgcgct gactttggaa gcctcccaat
     7921 gtttcaacgc ctcgccgaac gagccgtaac cgattaacgt attcaagcag caatacgcaa
     7981 aacaaaccca cgccaacgta ccgtccaaac ttccgatgtg tgccggttcg gcaaacggca
     8041 ggaacacggc ggcacttgcc gcataaatca acagcagaat ctgttgcggc ccgaattgcg
     8101 ccgacagcag cttttgcgcc acggcataac acacccatgc catactgcct gccgcacaca
     8161 gcaacacgcc cttcgcatac gcgcccaaac ccgacaactc gccgaattta tcgttaaaaa
     8221 acataagcaa accggcaagc agcaaaacca agccgatttt ctgagcggca gtcatccggt
     8281 ctttaaacac caacacaccg acaacaatca tcgtaaacgg cgaaatctgc cacaaaacct
     8341 gcgtcgtggt cggcgaaata taatgcagcc cttgggcaat cagcacaaag tttgccgaaa
     8401 tgcccgccac gccgagcagc agcagcctga atgagcacca agaaaaatcc cgccgcttcg
     8461 gcagccgccc gcccagtgcc agcaaaacaa acaataccgc cgccgccacg gtaaaacgca
     8521 cccacaccag cgtcggcgca tcgacaaact tcaatacctg ccgcacggca atcggcagcg
     8581 ttccccacgt catcgccgcc aaaagtgcca acgcgaagcc taggagcggc ctttggtttt
     8641 ccatcctgat tttcctattt ttaaacaacc gtattgccgg acgatgccgg tttgccgcat
     8701 cgggcaatga tggttcaagc gtttggcgtt tgattccaac cctttgattt caaacaaacc
     8761 ggctgaagct cggctattgc ttcgcgctat ttgaaaacac cgcctgaatt ttaaa
//
LOCUS       DAWISC010000073         8811 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWISC010000073 DAWISC010000000
VERSION     DAWISC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.073"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(144..479)
                     /locus_tag="WIW87_001203"
     CDS             complement(144..479)
                     /locus_tag="WIW87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ5174369.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYSALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAATKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            608..1513
                     /gene="mtrA"
                     /locus_tag="WIW87_001204"
     CDS             608..1513
                     /gene="mtrA"
                     /locus_tag="WIW87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ5174370.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1575..2060)
                     /locus_tag="WIW87_001205"
     CDS             complement(1575..2060)
                     /locus_tag="WIW87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prokaryotic membrane lipolipid attachment site
                     family protein"
                     /protein_id="HEZ5174371.1"
                     /translation="MKKYLIPLFAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKGF
                     RLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYEI
                     YLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQPL
                     K"
     gene            2207..3109
                     /locus_tag="WIW87_001206"
     CDS             2207..3109
                     /locus_tag="WIW87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5174372.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3150..4280)
                     /locus_tag="WIW87_001207"
     CDS             complement(3150..4280)
                     /locus_tag="WIW87_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5174373.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4485..7109
                     /gene="alaS"
                     /locus_tag="WIW87_001208"
     CDS             4485..7109
                     /gene="alaS"
                     /locus_tag="WIW87_001208"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ5174374.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPAVLDSVKDWVGAKLV"
     gene            7370..7801
                     /locus_tag="WIW87_001209"
     CDS             7370..7801
                     /locus_tag="WIW87_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174375.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            8101..8388
                     /locus_tag="WIW87_001210"
     CDS             8101..8388
                     /locus_tag="WIW87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174376.1"
                     /translation="MQVTSAEYLSNEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            8587..8673
                     /locus_tag="WIW87_001211"
                     /pseudo
     CDS             8587..8673
                     /locus_tag="WIW87_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
BASE COUNT         2288 a         2239 c         2077 g         2207 t
ORIGIN      
        1 tccactatat ttcacgctta ccccttgttt ctcaaatgcc gtctgaaata agcggcttaa
       61 tatattgttt acagtattgg gaagcataac agacaaaatg ccgtctgaaa tattttcaga
      121 cggcatttct tatccgaaac ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg
      181 gcataagtgt aagcggcacc cgcattcagg gcaatggcgg ttgccaatgc acctgcgatt
      241 tcgctgtcgg tcgcaccggc tttggtggcg gcggcggcgt gaacactgat gcagctctca
      301 caacgtgtag taatggcaac ggcgatggca atcagttcgc gtgttttagc atcaagtgcc
      361 tctgcagctg ccgcttgttc caatgcgctg taggcctgca gcattttagg atgcgcctta
      421 cccagctcgc cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt
      481 ttcttttcct ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg
      541 cgtattattg caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa
      601 aagatttatg gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga
      661 tgtgcagtgc cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg
      721 attggtacac attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc
      781 gcgtccggtc agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag
      841 ccacgacgga aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac
      901 ggtcaagcag tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga
      961 cacccacgcc gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc
     1021 gagtttacag tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac
     1081 ggtttccatg gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct
     1141 cgaacaggat aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt
     1201 gggacattta atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat
     1261 ggtggcggct gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg
     1321 actcagcccg cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa
     1381 aaaaaacccg gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca
     1441 cttcggcaag gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg
     1501 cgggcaaaaa taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc
     1561 atttgcgtac cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta
     1621 aatgcggggt tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag
     1681 gcattctgga tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc
     1741 gccgaacgca ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt
     1801 tttctgaagt tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc
     1861 tgatagccca agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag
     1921 gcaaggcgga aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg
     1981 atttccgtca atgtgggcac ataaatagac tggcagccgg aaagaactgc cgcaaaaaga
     2041 gggataaggt attttttcat gcccccatta taatcaagtt tgccttgaga aaacaaattg
     2101 ttcggcaaga aaaataaaat ttcggcatca gaagcaggca aaaacacatt ccacaagcct
     2161 tgccgcaagg tttacaatcc gaccgtcctt atcgcaacga ccgtttatgg ataccgcaaa
     2221 aaaagacatt ttaggatcgg gctggatgct ggtggcggcg gcctgcttta ccattatgaa
     2281 cgtattgatt aaagaggcat cggcaaaatt tgccctcggc agcggcgaat tggtcttttg
     2341 gcgcatgctg ttttcaaccg ttgcgctcgg ggctgccgcc gtattgcgtc gggacacctt
     2401 ccgcacgccc cattggaaaa accacttaaa ccgcagtatg gtcgggacgg gggcgatgct
     2461 gctgctgttt tacgcggtaa cgcatctgcc tttggccact ggcgttaccc tgagttacac
     2521 ctcgtcgatt tttttggcgg tattttcctt cctgattttg aaagaacgga tttccgttta
     2581 cacgcaggcg gtgctgctcc ttggttttgc cggcgtggta ttgctgctta atccctcgtt
     2641 ccgcagcggt caggaaacgg cggcactcgc cgggctggcg ggcggcgcga tgtccggctg
     2701 ggcgtatttg aaagtgcgcg aactgtcttt ggcgggcgaa cccggctggc gcgtcgtgtt
     2761 ttacctttcc gtgacaggtg tggcgatgtc gtcggtttgg gcgacgctga ccggctggca
     2821 caccctgtcc tttccatcgg cagtttatct gtcgtgcatc ggcgtgtccg cgctgattgc
     2881 ccaactgtcg atgacgcgcg cctacaaagt cggcgacaaa ttcacggttg cctcgctttc
     2941 ctatatgacc gtcgtttttt ccgctctgtc tgccgcattt tttctgggcg aagagctttt
     3001 ctggcaggaa atactcggta tgtgcatcat catcctcagc ggtattttga gcagcatccg
     3061 ccccactgcc ttcaaacagc ggctgcaatc cctgttccgc caaagataaa aaatgccgtc
     3121 cgaacatcct tcagacggca tatcgggctt tatttccccg ccttcacatc ctgccactgg
     3181 cgcaccataa acttcaatgc cgccggctgg ataggcacca tgataaagct gtttttcaaa
     3241 tcctcctcgg ttgggaaaat cgtattgtcg tttttaaatt cgtcttccat cagctcacgc
     3301 gcaggcttgc tcgaaggcgc gtaagtaacg aaattgccgt ttttcgccga cacttccggg
     3361 tcgaggaagt cgttgatgta tttgtgcgcg ttggcgacgt ttttcgcatc tttcggaatc
     3421 acgaaagaat ccacccaaat ccccacgccc tctttgggca tcatcacgcg gattttttcc
     3481 ttgccgcccg cttcttcggc acggcgtttg gcgatgttca aatcgccgcc gaaaccgatt
     3541 gttacgcagg tatcgccgcg cgccaaatca tcgataaagc cggacgaagt aaagcgtttg
     3601 atattggggc ggtttttctt gagtagggcg gttgcctccc tgatgtcttc cgtattgctg
     3661 ctgttcgggt ttttacccaa atagttcaac accataggat agatttccgc cgcgctgtcc
     3721 aaatagctga tgccgcattg cttgagtttg gacgtgtatt cggggtcgaa caccaaatcc
     3781 cactggttgt ccggcagctt gtccgtaccc aaagcctttt tcacgcgttc ggtattgatg
     3841 gcgaaggtat ttgtccccca ataaaacggc acggcgtatt cgtggccggg atcgaccccg
     3901 tccatcagcc tcatcatttc ggggttgagg tgtttataat tgggaatcag cgacttatcg
     3961 attttctgat acgcacctgc cttaatctgc ctgcccacaa acgcattgga cggcgcgaca
     4021 atgtcgtaac cggacttgcc tgtcagcacc ttgctttcca gcgtttcatc gctgtcgtac
     4081 acatcataag taaccttgat gccgtttttc ttttcaaaat cggcaacggt ttccggatcg
     4141 acatattccg accagttgta aattttcaat acgttttggt tttccgccgg tgccggtttt
     4201 tcggcaggcg gtttgtccga accgccgcac gctgcaagca gcaaagcagt caggacggcc
     4261 aggggcagat gtttggtcat tatcattcct tgcatatcgg gttggagaaa gcggccatta
     4321 tagccgatat tggcaacagg gcttcagacg gcattcaaaa tcccgccaca ctcttccgaa
     4381 aaccgccgct tccatagcta gaaacaggga tttgcggtaa gataccgccg ttcgttttcc
     4441 ctgcttttac catgacaaga catttgagag acattgaaaa aattatgaaa acctccgaac
     4501 tgcgccaaaa attcctaaaa ttttttgaaa ccaaaggcca caccgtcgtc cgctcttcca
     4561 gcctcgtgcc gcacgacgac ccgaccctgc tgtttaccaa cgcgggcatg aaccagttta
     4621 aagacgtatt cttaggtttc gacaaacgcc cgtacagccg cgccaccacc gcgcaaaaat
     4681 gcgtacgcgc aggcggcaaa cacaacgact tggaaaacgt cggctacacc gcccgccacc
     4741 acaccttctt tgaaatgatg ggcaacttct ccttcggcga ctacttcaaa cgcgacgcca
     4801 tccacttcgc ttgggaattt ctgacttccc ccgaatggct caacatccct aaagacaaac
     4861 tgttggcgac cgtttacgcg gaagacgacg aagcctacaa catctggttg aacgaaatcg
     4921 gtatgccgtc cgagcgcatc gtccgcatcg gcgacaacaa aggcgcgaaa tacgcatccg
     4981 acaacttctg gcaaatgggc gacaccggcc cttgcggccc ctgctccgaa attttctacg
     5041 accacggcga agaaatctgg ggcggcattc ccggcagtcc cgaagaagac ggcgaccgct
     5101 ggatcgaaat ttggaactgc gtatttatgc agttcaaccg cgacgaacaa ggcaatatga
     5161 acccgcttcc caaaccttcc gtcgataccg gtatgggctt ggaacgcata gccgccgtca
     5221 tgcagcatgt tcacagcaac tacgaaatcg acttgttcca agacctgctc aaagccgttg
     5281 cccgcgaaac cggcgcgccg ttcagtatgg aagaacccag cctgaaagtc atcgccgacc
     5341 acatccgctc ctgctcgttc ctgattgcag acggcgtctt gccttccaac gaaggccgcg
     5401 gctacgtatt gcgccgcatt atccgccgcg ccgtgcgcca cggttacaaa ctgggtcaaa
     5461 gcaaaccgtt cttccacaaa ctcgttgccg atttggtcaa agagatgggc ggtgcctacc
     5521 ctgaattgaa agaaaaacaa gcccaaatcg aagaagcatt gaaaaacgaa gaaagccgtt
     5581 ttgcccaaac gctggaaacc ggtatggctt tgttggaaaa cgcgctggtc aaaggcggca
     5641 aaacactcgg cggcgaaatc atcttcaaac tctacgatac ctacggtttc ccatacgact
     5701 tgactgccga catctgccgc gaacgcaata tcgaaccgga cgaagcaggc ttcgagcgcg
     5761 aaatggaagc ccaacgcgca cgcgcacgcg ccgcccaaag cttcaaagcc aacgcccaac
     5821 tgccttatga cggtcaagac accgagttta aaggttatag cgaacgccaa accgaatcca
     5881 aagtcctcgc cctctacaaa gacggcgagc aagtcaacga attgaacgaa ggcgacagcg
     5941 gcgcagtcgt catcgacttt accccgttct atgcagaatc cggcggccaa gtcggcgatg
     6001 tcggctatat cttctcaggc gaaaaccgct ttgaagtacg cgatacccaa aaaatcaaag
     6061 cggccgtatt cggtcaattc ggcgtacaaa cttcaggccg tctgaaagtc ggcgacagcg
     6121 ttaccgccaa agtggacgac gaaatccgca atgccaatat gcgcaaccac agcgcaaccc
     6181 acttgatgca caaagccctg cgcgatgtat tgggcagaca cgtcgaacaa aaaggctctt
     6241 tggttaccgc cgaatccacc cgtttcgaca tttcccatcc ccaagcggta actgccgaag
     6301 aaattgccga agtagaacgc cgcgtcaacg aagccatttt ggcgaacgtt gccgtcaatg
     6361 cagccattat gagcatggaa gacgcgcaaa aaaccggcgc gatgatgctc ttcggcgaaa
     6421 aatacggcga agaagtgcgc gtactgcaaa tgggcggttt ctctaccgaa ttgtgcggcg
     6481 gcacacacgt ttcacgcacc ggcgacatcg gcctcttcaa aatcatcagc gaaggcggta
     6541 ttgccgcagg cgtgcgccgt atcgaagcca tcaccggcct gaacgcactc aaatgggcgc
     6601 aagagcaaga gcgtttggtg aaagacatta ttgccgaaac caaagcccaa accgaaaaag
     6661 acgtactggc aaaaatccaa gcaggcgcgg cacacgccaa agcattggaa aaagaattgg
     6721 cacgcgccaa agccgaactc gccgtccacg caggcgccaa actcttggac gatgcaaaag
     6781 acttgggcgc agccaaactc gttgccgccc aaatcgaagc cgacgcagcc gccctgcgcg
     6841 aaatcgttac cgatttaacc ggtaaatccg acaacgccgt gattctttta gcggcagtaa
     6901 acgacggcaa agtctccctg tgcgccggcg tatccaaacc gttgaccggc aaagtgaaag
     6961 caggcgatct ggttaaattt gcagccgaac aagtcggcgg caaaggcggc ggcagaccag
     7021 atttggcgca agccggcggc acggatgccg acaaattgcc cgccgtgttg gatagcgtga
     7081 aagactgggt cggcgcgaag ctggtttgat gtgggaaagg cagcctgaaa ggtttcaggc
     7141 tgccttttgt gcaaagaggc cgtctgaaag gtctcgtttg ccgtaggttg ggtcgcgacc
     7201 caacaaattt tgtgaagtat aaaaatgttg gtcatgaccc aacctacctg cctttttgta
     7261 caaagaggct atctgaaagg ccttgtttgc cgtatggtgg gtcgcgaccc agcagatttt
     7321 tattagggta tgacccaagc tacttgctac gataaaaaag gatttttaaa tgagcattag
     7381 ccttattgga ctacacatta ccatagcaat cattttgttt tttactacaa attttatggg
     7441 aaaaaaatca tctatatttg gctattacca actgtctttt agcgaagaaa atcactctcc
     7501 ggcatttaat attttttaca gagcatttac ccctatatta tttatcgtta ttttttcttg
     7561 ggttgttact agtcttgaaa ttcccatttc tcttgaaaag ataaactatg tagtaattta
     7621 ttattttata attagattgt tatctgtatt tgtttttgag aaaacacaca tagttaactg
     7681 gtttaatcaa ctaacaatac ccatactatc cataacatta tcatttatag tatataacaa
     7741 aatgattttg cccaaaagtt ttctacttcc atcctcacaa gaagtagcta ctactttttg
     7801 aatagcgctt ggtggttaca tatataatat attaaataat gaatcagggc atttaaaatc
     7861 ttataaagaa agaagagtaa attatgtaaa acacatgcac aaaaaatttg aaagttattt
     7921 tggtaaaatt atagataaaa taatcaaaga ggatagttat aataatgatg attttttaac
     7981 cgataagaaa aaagcactaa tatattcagt tttaatttat gagaatttta ataggggact
     8041 agtttataga tattttgaaa aaaattattt tgtactggta gaataaaaac atttggaata
     8101 atgcaagtaa cctcagcaga gtacctttcc aatgaggaaa gtataaaaaa aggcggaaat
     8161 attcttatgg aaaaatacaa tgaaaaatat aatgaatcta ttgatggcaa taaaactctc
     8221 tataaatcat attatgaatc aagaagagag agtattaaaa actacaaccc agatgcaaaa
     8281 tacattaatg aaattgaatc aatttacatg atgcttggag aaatctatcc aaatgcacca
     8341 gacttcatgt caccacattt tgagggggac tgctctgagg gggaataaaa tcattattta
     8401 ttctttatta gttattagca ggatttgtcg ggcataaatg cccgacctac aaattcaatt
     8461 ttttcaaacc tctgccaaat attttcatct ttgcaaggct gtctgaaaac ccaaacccca
     8521 ttttcagacg gccttttttc gctaaaatcc ccataccgtt caatccgaaa acacaggaga
     8581 atcatcatgg aagttaccat ctccgccatc atcaatggcg aatttgccga ccaatacggc
     8641 aagcgcggta gtcagtttaa tgaaaacggg atgctgattt aattctattt cctttgaaac
     8701 taccaataac ctgcctccat cataaaacta aaagcaagcc gtagcctgca ttcccacaaa
     8761 ccgcgtgcgt tgccatgtca cacaccctac ctgcgggcga cgcaaacctt a
//
LOCUS       DAWISC010000074         8777 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWISC010000074 DAWISC010000000
VERSION     DAWISC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.074"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            106..354
                     /locus_tag="WIW87_001212"
     CDS             106..354
                     /locus_tag="WIW87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ5174377.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            351..737
                     /locus_tag="WIW87_001213"
     CDS             351..737
                     /locus_tag="WIW87_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ5174378.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WIW87_001214"
     CDS             complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WIW87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ5174379.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2597..3385
                     /locus_tag="WIW87_001215"
     CDS             2597..3385
                     /locus_tag="WIW87_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ5174380.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(3431..4030)
                     /locus_tag="WIW87_001216"
     CDS             complement(3431..4030)
                     /locus_tag="WIW87_001216"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ5174381.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            4158..4775
                     /gene="gmk"
                     /locus_tag="WIW87_001217"
     CDS             4158..4775
                     /gene="gmk"
                     /locus_tag="WIW87_001217"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ5174382.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4834..5040
                     /locus_tag="WIW87_001218"
     CDS             4834..5040
                     /locus_tag="WIW87_001218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ5174383.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            5132..7288
                     /locus_tag="WIW87_001219"
     CDS             5132..7288
                     /locus_tag="WIW87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ5174384.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMGIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WIW87_001220"
     CDS             complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WIW87_001220"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ5174385.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
BASE COUNT         2197 a         2020 c         2353 g         2207 t
ORIGIN      
        1 caaggtgtga ataaaacggc ggatataatc aaatatatgt aatgagattc ccaaaatctt
       61 ggtaatgatg tatattagtc aagttagcta atcatgagaa gtgttatgtt tcaagcaaat
      121 attcatcagg caaaaaccaa tttgagtcaa ttgattcaaa gagcagaagc aggggaaatc
      181 gttattattg cgaaggcagg taagccttgc gtccaactaa tcggtattga aaaaccggca
      241 cgaaatgcag gaaggttgaa aaaattcagt catatggaaa atacggatat ttcacgtatt
      301 cttgaggatg acaatgaaac ggcagcttta ttttttgagg agtcggctct gtgagaaaga
      361 ttctgcttga tacccatgcg ttgctgtggt ggttgttgga tgacaagaaa ctggggatat
      421 ctgcacgcaa actgatagaa aatccgagaa atgcgatctt tgtcagtgca gcaagtattt
      481 gggaaatttc catcaagcag aacaaggggt tgttgaaatt accggaagag ttttttgatg
      541 tgcttcaaga agaggatttt gaaatgttgc ctataggtct gtttcatgca aaacaggctg
      601 gaagccttcc ggagatacac aaagaccctt tcgataggat gttgattgca caaacccaag
      661 cggaaggctt tgaactgatg actgtcgatg aatatattcc gcaatatgga attagagttg
      721 tcaacgcatc aagctgacaa atacaaaaac tgtgtacaca agaaagaccg ccgaggcggt
      781 cttttcggtt attgtcttcg cttaaaagcc agacttctgt cgtggactat aggtgtgtcc
      841 cgatctgaat ctgccgcaca ataaggcaag gggattaaaa atgccgtctg aaaaatgttt
      901 cagacggcat aatggcttag gggttcaaca cgcgtgcgat gagggctttt tctttaccct
      961 gcatattggg gattttgacc tgaatgccgt tgcccggggc aacatcgaca ggctggcctt
     1021 tgcgggtcat ttgttccaat ttgatggttt ggttgccgct cgggtggatg atttcgagtg
     1081 aatcgctgac ggcaaagcgg tttttgactt ccactgttgc ccagccgttt tcatcgattt
     1141 cggtaacgtg tccgacgtat tggctttgtt tggcggtgga atggccggtc aggtagtttt
     1201 gataatcctg agtttggtgg cgttcgagga agccgctggt gtagccgcgg ttggcgaggc
     1261 cttcgagttc gctcaacagg ctgtaatcaa acggacggcc tgcgacggca tcatcaatcg
     1321 ctttgcggta ggactgggcg acgcgtgcaa cataatagag cgatttggta cggccttcga
     1381 ctttgaggct gtccacgccg attttggcga gtttttcgac gacttcgata ccgcgaaggt
     1441 ctttggaatt catgatgtag gtgccgtgtt cgtcttccat aatcggcatc atttcgcccg
     1501 ggcggttgga ttcttcaatc aggaaaactt tgtcggcgta gggatggcgt ttttgaccgt
     1561 tgatgccttc aaagttttgg ttggcttctt cttgggcttt ttcaaagttg aaaccttgca
     1621 gaagctgggc atcgcctgca tcgctttccg tggcattgtg aaccttgtaa tcccaacggc
     1681 aggagttggt gcaggtgcct tggttggggt cgcggtggtt gaaatagccc gacaataggc
     1741 aacggcctga ataagcgatg cacaatgcgc cgtggatgaa gacttcgagt tcgatgtcgg
     1801 ggcattcttg gcggatttcg gcgatttctt ccatactcaa ttcgcgcgac agaataatgc
     1861 gttcgacgcc gatgttttgc cagaatttca cgccccaata gttggtggtg ttcgcctgta
     1921 cggacagatg gatcggcatt tccggccatt tttcgcgcac ggtcataatc aaacccggat
     1981 ccgccataat cagcgcgtcg ggtttcatgg caatcagcgg ctccatgtcg gcaacgaagg
     2041 ttttgagttt ggaattgtgc ggcagggtgt tgacggttaa aaagaatttt ttgttgcgct
     2101 cgtgcgcttc tttaatgcct tgttctaaaa catcaagttt ggcaaattcg ttgttgcggg
     2161 cgcgcagtga gtaacgcggg ctgccggcgt aaacggcgtc tgcgccgtag tcgtaggcgg
     2221 cgcgcattct ttccaatccg ccggcgggca ataagagttc gggtgctttc atcgtgtttc
     2281 cttttcggtt gaaaccccgc cctttagggc agtagaatca gactttattt gggaggggcg
     2341 taaccccttc cgaatcaggg caacatatag ggcgacgctt tatgtgtcgt cctgtgtgtt
     2401 gaaacatgat ttgtatgctt tgtaaaaatg tttcagacgg cattgctgca aatgccgtct
     2461 gaaaagggtt tgaggatggg cggattatcc gcctttttgt attttcggtc aattttgacg
     2521 ggaaatttat aaggatttat caagtatttg cctgcttggg atataatgcg gtagtttgaa
     2581 tgcgagagag aatgtgatga atcctaaaat cgtgtttttc gacattgacg atacgctgta
     2641 ccgaaaatat acggatactt tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg
     2701 caaaggtata ttgacggcgt tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt
     2761 cagggatatg atggcggaag cgggaatgga tgccgtggta acgataaacg gacagtttgc
     2821 gctgctgcac ggaaaaaccg tgcgcgaagt accgatggat gccggtttga tgggcagggt
     2881 ttgcgcgcat ttggatggct tgggcatgga ttatgcgttt gtcggcggag aggggatcgc
     2941 cgtgtccgcg ctgtcggaat gcgtgtgccg cgccttgcag catatcgcca gcgatttttt
     3001 tgccgataag gattattttt caagcaaacc ggtgtatcag atgctggtgt ttgcggagga
     3061 aaacgaaatg ccgctctggt cggatattgt ggaacaggaa ggcttgaaaa cggtgcgctg
     3121 gcacgaggaa gcggtcgatt tgctgcctgc gggtgcgtcg aaaacagacg gcatcagaag
     3181 cgtggttgaa gcattgggtt tggaaatggc ggacgtgatg gcgttcggcg acggtttgaa
     3241 cgatgtggaa atgctgtcag aagtcgggtt cggcgtggca atgggcaacg gggaacaggc
     3301 ggcgaaagaa gcggcgaaat atgtttgccc cagcgttgat gaagacggcg tgttgagggg
     3361 cttgcaagat ttgggcgtga tttgaacgca tcataacaac cctgccggtt tcagacggca
     3421 gggtcggttt tcagcccttc atacagcctt cgttttgaag cagggtaaat aagggcgcgc
     3481 cgcttgcacg gatattcttg ccgccttgaa ggtcggtaaa ttccaaaatg gcggcagctt
     3541 cgacaatttc cccgccgagt ttgcggatca gttccagccc ggcaagcatt gtgccgcccg
     3601 tggcaaccaa atcatcgacc agcagcacgc gcgaaccggg tttgacggca tcggtgtgga
     3661 tttccaccgc agcttccccg tattcgagcg cgtagctttg cgatacggtt tcaaaaggca
     3721 gcttgccttt tttgcggatg gggacgaaac cgacgttgag ctggtaggcg agtgccgcgc
     3781 cgataatgaa gccgcgcgcg tccaaaccgg caacgatgtc gattttctga tccatatagc
     3841 ggtaaaccaa taaatcaacc aaaagtcgga agtattccgc gctttgcagg acgggcgtga
     3901 tgtcgtggaa taagatgcct ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa
     3961 gcgcgccgac actcatagct tcgggatgaa ccagcattgc gtgttccaag tttgatgttt
     4021 ttaacgccat attgtagccg tcagcctgcc gcttgccaaa tcaatcaaaa caatagcggc
     4081 aaaatatggg catcggcaaa taggaaaccg agtgtccgtt gtgatacaat cggctggaaa
     4141 atatacaggt aaaaaatatg tccgcttatc gaaaaggcaa catctttatc atttcggccg
     4201 cttccggcac gggcaaaacc acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc
     4261 gcgtttccgt atcgcacacg acgcgtccgc cgcgtgaagg cgaagtaaac ggcgtacatt
     4321 atcattttgt ttctaaagaa gagtttgagt cgcttatcgc gcaggaagct tttttggaat
     4381 acgcagacgt gttcggcaac tattacggca caagcgcgga gggcgtgaat gctttggcgg
     4441 cggcaggcta tgacgtaatt ttggaaatcg acgttcaggg cgcggcgcag gttcgcaacg
     4501 cgctgccgga agccgtcggc atctttatcc tgccgccgtc tttcgatgtg cttgctgcac
     4561 gtctgaacgg acgcgggacg gacagtcggg aagttatcca aaggaggctg tcgaaggcaa
     4621 ggcatgaaat cgaacagtcc gtattgttcg attttgtcgt ggtcaatgat gacttggcgc
     4681 gggcggagga ggatttgcgc catattgtga atgcctgccg tctgaaaagg tcgcggcaac
     4741 tgggatttat tgcagatttg ttggaaaatt cctaaaaaac cgcgaaaata cccagtttcc
     4801 tattttaaat atttttgaaa gaaagcaaat aatatggcac gtattaccac cgaagactgt
     4861 accggaaaaa tttccaacca ttttgacctg acattggtag cggcccgtcg ggcccgccag
     4921 cttgaaaatg gcaatacgcc gcttgtggac gatgtccgca ataacaaacc gaccgttacc
     4981 gccttaaggg aaatcgccgc cggacatatc ggtacagaac tgttgacgcg caataaataa
     5041 attctgccgg aaacgcacgc cggaacactt tgccgccgtg cagtccgacg gtttgaaatg
     5101 aaaaacacat acataccgaa agccgtctga aatgcccgcc ccccaacctt ccgcccctta
     5161 cgaccccctg accgccgaag cgcgtgccct gcttttccat accgcctcct acctcaagcc
     5221 cgaggaacag gcggagcttg aaaaagctgt cgcctatgcg tttcgtgccc acgacgggca
     5281 aacccgcaaa agcggggagc cctacatcac gcatccgatt gccgttgcga cgcagctcgc
     5341 cctttggcat atgggcatac agggtctttg tgcaggcgtg atgcacgacg tattggaaga
     5401 tacgggcgtg acaaaagggg aaatggcggc ggtgttcggc aatacgattg ccgagatggt
     5461 ggacgggctg tccaaactcg agaaactcaa atttgaagat catgcggaac atcaggcgga
     5521 gagtttccgc aaactgattt tggcgatgac caaagatgtg cgcgtcatcg tcgtcaaact
     5581 tgccgaccgc ctgcacaata tgcggacgct cggttcgatg cgcccggaca aacgccgccg
     5641 gattgcaagg gaaacccttg aaatctatgc acagattgct aaccgtatag gtttgaataa
     5701 cgcatatcaa gagcttcagg atttatcgtt ccaaaacctg catcccaacc gctacgagac
     5761 tttaaaaaaa gcgatggaca agagccggaa gaaccggcag gacgttgtcg gcaaagtctt
     5821 gcgcgcattc ggccagcggc tggtaggcgc gaatatagag gccaaaatca aaggcaggga
     5881 aaaaaacctg tacggcatcc atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt
     5941 tatggatatt tacggtttcc gcgtcattgt caacagcatt ccagcctgtt atgccgcact
     6001 cggcgcattg cacaccctct atcagcccaa gcccgggcgg ttcaaagact atatcgccat
     6061 tccgaaaagc aacgggtatc aaagtctgca tacgactttg gtcggtcctt acggcttgcc
     6121 gattgaagtt cagatacgta ccaaggaaat ggatgctgtt gccgaaggtg gaatcgccgg
     6181 acattggagc tataaatcat attctaagac ggtcgatcag gcggtgcttc acacaaaccg
     6241 gtggctgaaa aatatcttag atttgcaggc aagcagtgcc aatgccattg agtttctcga
     6301 acacgtcaaa gtcgatttgt ttccgaacga aatctacatc cttacgccaa aaggaaaaat
     6361 cctaactttg cccaaagggg caacgcctgt cgattttgct tatgcggtgc ataccgatat
     6421 cgggcacaaa accgttgccg cacgtatcaa caatatcatg atgccgttgc gtacgaagct
     6481 caaaaccggt gattctgttg aaattatcac atccgaacac gccaaaccca atcccgcgtg
     6541 gttgaatttc gccgtgtcag gcagggcgcg cagtgccata cgccaatata ttaaaaacct
     6601 taaccggcac gatgcggtcg ttttgggaga gagcctctta caaaaagccc tgtccagttt
     6661 gctgcccaaa gatgtcctgc tttcagacgg catcaaggaa aaatatcttg ccgatctcaa
     6721 cgacaaacag acttcatttg aggaagtgct gtacaacgtc ggtatggggc ataccctgcc
     6781 tgtttatgtc gccatgcaca ttgccgagtt ggcaggggag catttcggta gcgaggtcag
     6841 gctcagttcc attaaagtcg atggtcagga aagcgggcat attcattttg cagagtgctg
     6901 ccaccctgtt cccggcgatt ccatccgttt gctgttggtt aagggaaaag gcatgattat
     6961 ccatagggat acctgcccga cgttgttgaa gtccgatccc gaacagcagc tggatgcaga
     7021 ctgggaaaat atgaacgggc agaactaccg tgtcgggctt caagtccaat cggaagacag
     7081 ccacggcttg ctggcattaa tggcgcaagc gatttccgat tccggtgcgg acattgagtc
     7141 ggtcgaaaca ccgtctaaat cccagtcggg aacggaaggt tttgtcgaat tcaaattctt
     7201 attgaaagtc aagaatctga atcaattgaa tcagattatt caaaatctgc atagtattcc
     7261 atatatccgc aaagtcatca gaagttgaag cagataaaac gggttgtctt ttgattaaca
     7321 ggcaacccgc tttatggttt atccgttcaa ttgcttattt gttcaaatat acggcaagcc
     7381 tttcaacaat ctgcatcgcc gcttcatctt tacttgtttc gggaaaagat agttccccgt
     7441 cgtccccgat aatggtaatc cggttggtcg gtttgcccat cgcgattgaa acatcattgg
     7501 caactatcac cggtattttt ttcttaatac gtttttctcg tgcatatgcc attacattct
     7561 ccgtttcagc ggcaaaaccg atgcagaacg gcgggtttgg caatgaggca atagaagcca
     7621 aaatatcggg gttctcatcc aattcgatgg ataacggttt ggcattttta tcttttttga
     7681 atttttgcgt actcttgttt ttaaccttat agtctgagac ggcggcaaca gaaataaaag
     7741 catcttgctt ttcaatcaaa cgatgcactg cgcgatgcat atcttcagcg ctgacggctt
     7801 gaaccgtatc ggatatgccg aaaggcagcg cggtttgaag ctgtccgtga atcaggctga
     7861 cttccgcacc ggcggcacgg cacgcccgcg ccaaagctac ccccattttt ccgctggagg
     7921 tatttgtgat gcccctgaca gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga
     7981 ttttcttgcc ccttaaaatt ttcggtgtcc acaagtccgg aagcaaatcc agcaattcgg
     8041 caggttccgg catccttccc ataccatttt ctccgcaagc ctgttcgcct aagcccggca
     8101 tatagacagt aatgccgtct gaaaccagtt gtgcgatatt ccgttggttg gcagggttga
     8161 gccacatttc cacattcatc gcgggagcga tggcgagtgg acatttccgt gcggctgcca
     8221 gactggtcag taggttatct gccacgccgt tacagatttt tgccacggta ttcatacttg
     8281 ccggcgcaat cagaaaaaca tccgcattcc gggtcaggtt gatatgttcc ataccgtttg
     8341 aaccgttgcc gccgtgcgtg tcggtcagga caggattgcc gcttaaagcc tgaaaagtca
     8401 gcggagaaac aaattcagtt gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt
     8461 ttttcagcag acgaaccaac tcgcaagact tatacgccgc aatactgccc gttacaccta
     8521 aaagaatatg tttacccatt ttgacaattt ctaattacga agaaaaaaca gcagccattc
     8581 tataacaaag tgcggataca ttagttaccg tttggataca aaatgccgtc tgagtccccg
     8641 agtttcagac ggcatattca caaaggcgca ccagtcagag gagggggagg aagggattgt
     8701 tggaggcggc gcagcattta gcagaaataa aaaaccttat ccgacagcga catgacgaat
     8761 ttccccaaaa aaatccc
//
LOCUS       DAWISC010000075         8769 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWISC010000075 DAWISC010000000
VERSION     DAWISC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.075"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(41..1132)
                     /locus_tag="WIW87_001221"
     CDS             complement(41..1132)
                     /locus_tag="WIW87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ5174386.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGDGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNNYLNDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            complement(1267..1815)
                     /locus_tag="WIW87_001222"
     CDS             complement(1267..1815)
                     /locus_tag="WIW87_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ5174387.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(1970..2341)
                     /locus_tag="WIW87_001223"
     CDS             complement(1970..2341)
                     /locus_tag="WIW87_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ5174388.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            complement(2397..2504)
                     /locus_tag="WIW87_001224"
                     /pseudo
     CDS             complement(2397..2504)
                     /locus_tag="WIW87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WIW87_001225"
     CDS             complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WIW87_001225"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ5174389.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGNTPEEILTNLQGHHYQNKDITQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4494..4590)
                     /gene="ffs"
                     /locus_tag="WIW87_001226"
     ncRNA           complement(4494..4590)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIW87_001226"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4851..8684
                     /locus_tag="WIW87_001227"
     CDS             4851..8684
                     /locus_tag="WIW87_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ5174390.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
BASE COUNT         1931 a         2391 c         2430 g         2017 t
ORIGIN      
        1 gcgggaatga cgcggtgggg gtttcggttt ttccgcctgt ttattttgcg gcttcgattg
       61 ccgctatttc tttgcgtagg tgtttgatag cgggggttac gatggcaaca aacattgctt
      121 cgcggacgcg cctttgggcg ggactgcgca tcaggtagcc ttttgcgccg gaatgcaggg
      181 cggcggattg ggcggcggca agtgccagta cggcggcggc ttcgcgcagc ttgagggtag
      241 caaggttgtc gggcgtgccg ctccaagcca agccggcgag ccgttcggtt tctgcccacg
      301 cgccgtccag ccttgttttg aggctgtcgt agccgtcgtt gaggtagttg ttgacttcgg
      361 cgttgacgac gttggcgagg cggatgatgc cgaggctgcc gtcgattacg cccgcgccga
      421 tgccgatttg caggaggata aagcctgctt tgatgctttg gatgtagtcg gcaaactgtt
      481 cgggcgcggc gatgatgtct tcgtcgggga taaatacgtc tttgaaattc aggctgaagg
      541 tgcgcgtacc ttcgagggcg caaaattcgg ggcagttttg caggcttacg ccttcccatt
      601 gtccgcctgt gatgaacata acgtagccgt cgccgatttg ggcggtattc gcccagatgt
      661 ggtcttcacc gatgttggac acccacggca gcgcgccgtt gactgtgtag ccgccttcca
      721 cgcgttcggc ttggaggttg tgtttttcga tgtcggcaag gtgtttgacg gtattggaca
      781 tgcccgtacc cgccaatact ttgccttgca ggatgtcggc aaggtatttg tctttgacgg
      841 cccggttggg cgtttggtgc agataccacg cgcaagccgc ctgacaccac gcactgaaag
      901 aggttgcgcc gcattctttg ccgatttcgc gcaatacggc gatttgcgtt gccaaaccca
      961 agccgttgcc gccttcggct tctgtaccga ctgcgccgaa tccaccgatt gcgccgagtt
     1021 cgcgcataaa tgcttcgggg tagtatcctt tgcggtcgat gtcgtccact atgggtttga
     1081 gcttggtttt gacgaattcg gcaacgttgg caatcagggt ttgggcgttc atctttgttc
     1141 cttaaggttt gcggggaaat cgggggcgcg cctgatgcgc ggctgcttcc ctgccgctta
     1201 ggcggcttgt ttttgtttga gggggtgttt ttgaaaaacc gcccgatatt cgggcggttt
     1261 gccgtatcag gcgtaagcct gcaattcggg gttgatttcg gtttgtccga ggttgttgac
     1321 gtagttgcac agggttgcca aggctacgcc catcacgact tcgactgcct gctgctggtt
     1381 gtagcccgca tcgaaaaatg ctttgagttc ctcgtcggat accgcgcctt ttttcgccat
     1441 tacggcttgg gtgaaggcgg cgagcgcgcc gagtttggca tcgtcaaatt cgcctgctgc
     1501 caaagcgcgc gcggctttga cggattgttc ggacaggagt tttttcaggg ttgcgagttt
     1561 ggtgtgccct gccacgcaaa aaccgcattg gttggtacgg gcggcgatga tctggatgac
     1621 ttcgacttcg ccggcggtca ggctgttggc ggcgttgagc ttgccgactt cttggtaaaa
     1681 cgccaaggct tcgggggcgt ttgataatac gccgataagg ttggggataa agccgttgtt
     1741 ttgaagtacc gcctcgacgc gcgctttggc ggcttcgggg gcggtttcga gggtgtgtac
     1801 ggttaaacgt gccattttct ttccttgttc gcaaatattg ggtacgggcg catggtatgc
     1861 atttcggaac ggaataggaa agactgattg gttatgtgct gcaaacaaaa ggttataaga
     1921 aatgccgtct gaacattttt cagacggcat gatggaaaag aaaacgcgct tatcggcccc
     1981 cgcgcccgaa atattgcgcc aatgcggctt ggggattggc tgcctcaacc gtttcgggca
     2041 attcggtata gccgcgcgat aaaaggtggc gtgcataaag gctgttgccc tttgcgccga
     2101 accagacgga aaccgccaag cccaatacgt taaacgccga gtctgtggcg ggcgtgtcgc
     2161 cgtaaaccag ttgggcaaac acagccagca cgaatatcgc gcccgtcagc cccagcccca
     2221 aaccccacat tcttttgaaa cacgcccaca gcgtgccgaa caagagcccc ggccatgacc
     2281 agccttgttt gacggcttgg ggcggcaggg cgggatgggt gtagattttg tatggtttca
     2341 tcgtgtttcc ttttcggttg aaaccctgcc ctttgggaag gtaggatcag actttatagt
     2401 ggattaaatt taaaccagta cggcgttacc tcgccttgcc gtactatctg tactgtctgc
     2461 ggcttcgttg ccttgtcctg atttaaattt aatccactat atttgggagg cgcgcgcgcc
     2521 tgtgccggca tacggcttga aagcgattac ccgatgggga acttcaaacc cgacaatgcc
     2581 gtctgaacgg tgtcttgcct tcagacggca ttgcctgcct tcaaagcgga cgcgcttatt
     2641 ccgcccagtt tttctttttg ctggttttgc ctacgcccgg gttgaagctg ttggtcgggt
     2701 caagtttgcg gtaaaactgt ttgagcgcgg gcttggcttc gtacaaatgg ccgacgttgt
     2761 gttcggctgg atattgcgcg ccgcgttgat ccaagagatg cagcatttcg tgttccaatg
     2821 ccatgcagtc gttgcctttt ttgatgatgt aatcctgatg gaaaacgtgg cacatgaaat
     2881 gtccgtagta gagcttgtgg atgattttat tgtcgatttc cggcggcagt ttttcaaacc
     2941 agtcgcggtc gtcgcggcgc agggcgatgt caagcgcgac caagtcctcc acttcgtcgt
     3001 cgtgtacggc acggtagcgg atggcggctg aggcgacggc gaaacggtgc agcatcgcgg
     3061 cttgggtttc ttcggcgttg cactcgaaaa acgcgccgcc gtgatgtgca aaatactctt
     3121 tcaggaacgc gcgcgcctca tccacgcctt ttccgcccat tttcagaatc aggtggtgtt
     3181 cgtatttgtc gcggtaatcg cgcatggatt tgggcagatg gtcaggcagg aatttgctga
     3241 cgaactgcat tgccttgtcg gaaaaatgtt tgggcaggaa gctgactttt ttgccgaacc
     3301 tgtccacgcg tgccttcaaa tcaaataatt tcggcagttg gtgcgtaccg aattttttga
     3361 tgacgtaaaa cgtatctttg ccgtacacgt cggcaatgtc gaaagcgtgg cggtggatgt
     3421 attcgccgga aacgggcagg ctttcaaatt cgcccaaggc ggcgcggcgg atgtcggtca
     3481 gctcgttgat gtcgttcgtg ccgatgtaga acacggccgt ttgtttttct tgcggaaagg
     3541 tatccaagcg gacggcgaat accatcagct tgcccgcgca gcccgaggct tcgtaatggc
     3601 gcgctgggtc ggcattgaaa cgcgcggcgg tcggttcgtc cacttggcgg acatgttcgc
     3661 aataggcgtg gtcgtgtcct ttgcccgcgt cttgcgtgat gtctttgttt tgataatgat
     3721 gaccttgaag attggtcagg atttcttcgg gcgtgttgcc caagtctatg cccaagtggt
     3781 tgaccagttc caacctgcct tcttcgttga tttgggcgaa caacgccatt tcggtgtagg
     3841 ccgggccgcg ctgtaccaac gcgccgccag agttgttgca cacgccgccc aagacggacg
     3901 cgccgataca ggatgagccg ataaccgaat gcggttcgcg ccccaaaggt ttcagcagca
     3961 attcgagctg gttcagggtc gagccgggca ggcagacgac ttgttcgttg ttgttgatgg
     4021 tttggatgat gttcatccgc atggtgttca caatcacgat gtcgcggtcg taatcgttgc
     4081 cgtcgggggt cgagccgccc gtcaaaccgg tattcgccgc ctgcgtaatc acaatcacgt
     4141 ctgcttcgac gcacgcctgc agaattttcc acatttccag aatgcttccg gggcgaacca
     4201 ccgccaacgc cttaccctcg ccgaagcggt aaccttggcg gtattgttcg gttttcgcgg
     4261 ggtcggtgat gatgtatttt tcgcctacgg tttgggtcag tcttgacagt aattgtgatg
     4321 cgctcatggc agtttcctta aaattgtcgg caggtgcatt gcacattgga attgttttca
     4381 cattgtagtt atacgttatg gcaaagtaaa gaaaatgccg tctgaacggc tttcagacgg
     4441 catcggtgcg atacgggaac gccggaacat cgaagctccg gcgtttcaaa tagggcggcg
     4501 ggccaaaccc ccggcactgg cgcattggag tgggctgctg gcttccgccc ctgacccggt
     4561 gttccgattt gccatgcggg gagacccgcc tcagagaaac ggcattataa cgggttttct
     4621 gaaaaactca accgttttga tacggtcata cgccggaaac accacctaaa atttatattt
     4681 gataatattg tcaacaattt ctcaaagcgt tattttgttt ctataagggt atttcctgtt
     4741 tcggcattga aaagtatcaa aaattgaact acattatcgc cttttcaaac tcgcctgaaa
     4801 ccgacttttc agacggcatt caaataaaaa ctgccaaaca cggacacacc atgaccacga
     4861 ctaccgcccc tcagcgtatt cgggaaatcc cctacaacta tacttcctac accgaccgcg
     4921 aaatcgtcat ccgattattg ggcgacgaag cgtggcaaat cctgcaagac ctgcgcggtc
     4981 aacgcaaaac cgggcgttcg gcgcggatgc tgtttgaagt gttgggtgat atttgggtgg
     5041 tcgtgcgcaa tccgtatctg gtcgatgact tgctggagca cccaaaacgc cgcgccgcgc
     5101 tggtacgtga aatgcgccac cgcttaaatg aaatccgcaa acgccgcgac gataatcggc
     5161 aagtggatgt gttggttgcc gcagcagaaa aagcagtcga gcgttttgat agcagttttg
     5221 atgaaaccag ccaaaaacgg cggcagattt tggagcgttt gagcaaaatc accaagccgc
     5281 acaatattat gttcgacggg ctggcgcggg taacgcacgt taccgatgca accgactggc
     5341 gcgtggagta tccgtttgtc gtcgtcaatc ccgacacgga ggctgaaatc gcgcctttgg
     5401 tgcgcgcctt aatcgagctg gatttggtca ttattccgcg cggcggcggc acgggttata
     5461 ccggcggcgc gattcctttg gacgcaaaca gcgcagtcat caataccgaa aaactcgaca
     5521 agcatcgtgg tgttgaatac gttgagctgg caggcttgga cggcaagcat ccgattatcc
     5581 ggtgcggcgc gggcgtggtt acgcggcggg tggaagaaac cgcgcatcag gcaggtttgg
     5641 tgttcgccgt cgatccgact tctgccgacg cgtcatgcgt gggcggtaat gtggcgatga
     5701 acgcgggcgg caaaaaagcc gtgctgtggg ggacggcgtt ggacaacctc gcctactgga
     5761 acatggttaa ccctcaaggc gaatggctgc gtatcgagcg cgtgcgccac aatttcggca
     5821 aaatccacga cgaagaaacc gccgtgttcg acgttcacac gctggattca gacggcatca
     5881 atatcgttaa aaccgaacgc ttggaaatcc ccggccacaa attccgcaaa gtcggtttgg
     5941 gcaaagacgt taccgacaaa ttcttgagcg gcctgcccgg cgtgcaaaaa gaaggtacag
     6001 acggcatcat caccagcgtt gccttcgtgt tgcataaaat gccgaaatac acgcgcaccg
     6061 tgtgtatgga gtttttcggt acggtcgcca ccgccacgcc atctattgtc gaaatccgcg
     6121 actttttgct tgcccatgaa agcgtgcggc tggcgggttt ggaacatttg gactggcgtt
     6181 atgtccgcgc cgtcggctac gccaccaaag cggcgggcaa gggacgaccg aaaatggttt
     6241 tgctggcaga cgtggtttca gacgacgaag ccgccgtaga ggcagccgcc gaacacatct
     6301 gtgaactcgc acgcgcccgc gacggcgaag gctttatcgc cgtatcgccc gaagcccgca
     6361 aaaccttctg gctcgaccgc agccgcaccg ccgccatcgc caaacatacc aacgccttta
     6421 aaatcaacga agacgtggtc atcccgctcg aaaggctcgg cgagtattcg gacggcatcg
     6481 aacgcatcaa cattgagctt tccatccaaa acaagctcaa actctgtgcc gccttggagc
     6541 aatatctttc gggcaaactc cccatcgaca aaatgggcac tgacctgccg accgccgaac
     6601 tgttgggcga acgcggcaaa cacgccctgg cccacgtttc cgccgtcaaa acgcgttggg
     6661 aatggctgct cgccaatctt gacacgccgc ttgccgacta caaagcccgc tacggcgcag
     6721 ccgtccacgc cgcacccgaa gccaaaaaca atgaaagctg ctttattgcc ttccgcgatt
     6781 tccgcctgcg cgtgtctgtc aaagcggacg taatgaaacc gctttctgaa atcttcagcg
     6841 gcaaaaccga caccaaaatt atccaaggct tgggaaaaat ccacgcaaaa accgtacgca
     6901 gccgcgtctt tgtcgccctg catatgcacg ccggcgacgg taacgttcac accaatattc
     6961 cggttaactc agacgatgcc gaaatgcttc agacggcata ccgctcagtc gaacgcatta
     7021 tgaaaatcgc ccgttcgctt aacggcgtga tttccggcga acacggcatc ggcattacca
     7081 agctcgaatt tttaagcgac gaagaaatgc agccgttttg ggactacaaa aaccaagtcg
     7141 atccgaaaca caccttcaac cgtcacaaac tgatgaaagg ctcggactta cgcaacgcct
     7201 acacgccgtc cttcgagctg ttgggcgcgg aatcgctgat tatggaaaaa tcaaacctcg
     7261 gcacgattgc cgattccgtc aaagactgcc tgcgctgcgg caaatgcaaa cccgtctgct
     7321 ctactcacgt tccgcgtgcc aacctgctgt acagcccccg caacaaaatc ctcggcgtgg
     7381 gcttattgat cgaagccttc ttatacgaag aacaaacccg ccgcggcgtt tccatcaaac
     7441 actttgaaga actcatggac atcggcgacc actgcaccgt gtgccaccgc tgcgtcaaac
     7501 cctgccccgt caacatcgac ttcggcgacg ttaccgtagc cgtccgcaac tatcttgccg
     7561 attccggcca caaacgattt gcgcctgccg cagctatggg tatggcgttt ttgaacgcca
     7621 ccggcccgaa aaccatcaaa gcccttcgcg ccgccatgat acagatcggc ttcccagcgc
     7681 agaatttcgc ctacaaaatc ggcaaacttc ttccaatcgg cacgaaaaag caaaaagccg
     7741 aacccaaggc aaccgtcggc aaagccccga ttaaagaaca ggttatccat ttcatcaacc
     7801 gcccactgcc caaaaacgta cccgccaaaa caccgcgctc cttattgggc atcgaagacg
     7861 gcaaaagcat ccccatcatc cgcaaccccg ccgcgcccga agatgccgaa gccgtgttct
     7921 acttcccggg ttgcggctct gagcgtctgt tcagccaaat cggacttgcc gttcaagcca
     7981 tgctctggca cgtcggcgta caaaccgtcc tgccgcccgg ctatatgtgt tgcggctatc
     8041 cgcaagacgc aggcggcaat aaggcaaaag ccgaagaaat gagcaccaac aaccgcgtgg
     8101 ctttccaccg tatggcgaac accctcaact acctcgacat caaaaccgtc gtcgtcagtt
     8161 gcggcacttg ttacgaccag ctcgaaaaat accgctttga agaaatcttc cccggctgcc
     8221 gaatcatcga catccacgaa tacctgctcg aaaaaggcgt gaaactcgac ggcgtaaaag
     8281 gtcagcaata cctctaccac gacccctgcc atacccccat caaaaccatg aacgccaccc
     8341 aaatggccag cagcctgatg gggcagaaag tcgttttaag cgaccgctgc tgcggcgaat
     8401 ccggtatgtt tgccgtcaaa cggccagaca tcgccactca ggtcaagttc cgcaaacaag
     8461 aggaaatcga gaaaaacctc aaagagctgc cgcagggcga acccgtcaaa atgctgacct
     8521 cctgccccgc ctgcctgcaa ggcttgagcc gctacgccga cgacaacaat atgcctgccg
     8581 actacatcgt catcgaaatg gcgaaataca tcctcggcga aaactggctg gatgagtttg
     8641 taaaaaaagc caacaacggc ggtgtagaga aagtgttgct gtaacaacgg acacggaaat
     8701 gccgtctgaa cgccgaaagc cttcagacgg cattgtttga accaaatata gtggattaac
     8761 aaaaatcag
//
LOCUS       DAWISC010000076         8720 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWISC010000076 DAWISC010000000
VERSION     DAWISC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.076"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            191..1726
                     /locus_tag="WIW87_001228"
     CDS             191..1726
                     /locus_tag="WIW87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ5174391.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WIW87_001229"
     CDS             1723..1815
                     /locus_tag="WIW87_001229"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ5174392.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WIW87_001230"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WIW87_001230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ5174393.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRINPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYHYAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="WIW87_001231"
     CDS             complement(3106..3276)
                     /locus_tag="WIW87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ5174394.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WIW87_001232"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WIW87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ5174395.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GAQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            3697..4713
                     /locus_tag="WIW87_001233"
     CDS             3697..4713
                     /locus_tag="WIW87_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ5174396.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="WIW87_001234"
     CDS             4739..5158
                     /locus_tag="WIW87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ5174397.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="WIW87_001235"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="WIW87_001235"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ5174398.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPGKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8648
                     /gene="polA"
                     /locus_tag="WIW87_001236"
     CDS             5832..8648
                     /gene="polA"
                     /locus_tag="WIW87_001236"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ5174399.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTEEGRKVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2039 a         2257 c         2300 g         2124 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagcgtcgcg atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacaaac tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaggc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt cgtcttggta aacaatgccg
     2401 acgccgccgc ctaagtctaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcata gtggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt ttgcatcgac atcggggttg atgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgtgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctgcccag cgaggcgaag tgtttgataa tgctcagatt gccgtttgcc
     2941 ttgacggcgt aacagacgag cgggttcaaa gcggcaaacg cggtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcagcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgcggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
     3481 cgcccaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgcaaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacgcgcg acggacgcga
     3601 tttttatgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tggaaattgc
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggactgatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcgacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgttgtat ggttttcgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgcggt tgtcactgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt tactaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagtgt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttagaa tcattttcca aacatatccc ttatggtgtc gtctttgaag acgacggcga
     4861 cacaggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatctataa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcgtc ttggatgatg aattgctgga caaaatccta tcccgaaaat ctacataacc
     5161 ctcacaaaag gatacccaaa tgcccctact agacagtttc aaagtcgatc acacccgtat
     5221 gcatgccccc gccgtacgcg tggcgaaaac catgactacg cccaaaggcg acaccattac
     5281 cgtgtttgac ctgcgctttt gcgttcccaa caaagaaatc ctgcctggaa aaggcataca
     5341 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagtc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtccgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggatcg tcctacctct
     5881 accgtgcgta tcacgcgatg gggcaaaacc tgaccgcccc cgacggcgcg ccgacgggtg
     5941 cgctgtatgg tgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacaggctgg ccggtattgg tgattgggca ggtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
     6301 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
     6421 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgct ggctggtgtg atggaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc cgcgctgccc caactgccgc
     6541 tgtcgtatga tttggtcacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact ggattatcaa gccgttacca
     6841 ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg ggcggacaca atcggcatcg
     6901 atacggaaac cacgtcatta gacgcgatga acgcctcgct ggtcggcatc agcatcgctt
     6961 tccaagcagg cgaagcggtt tacatccccg taggacacag cctgaccgcc gcgcctgaac
     7021 agcttgattt acaagacgta ttaggccgtc tgaaaccgca tttgggaaac cccgccctaa
     7081 aaaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gccatgctcg cttcctacat catcgagagc catctcggac
     7201 acggcttgga cgaattgtcc gaacgctggc tcggcttgga aaccattacc tacgaatcgc
     7261 tgtgcggcaa aggcgcgaag caaatcggtt ttgccgatgt cgccatcggg caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga agcgcacctg cgcgcgcaaa
     7381 tggacgaaaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caggtattgt
     7441 ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aggaagccta tgccgccgca ggccagccgt
     7561 tcaacctcaa ttcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccca
     7621 ccaaaggcct gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac
     7681 agctcgcgcc cgactacccc ctgcctaaaa tcatcctgca aaaccgcagc ctggcgaagc
     7741 tcaaatccac ctacaccgac aaactacccg aaatgatttc ccccaaggac ggccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc
     7861 tgcaaaatat ccccatccgt accgaagaag ggcgtaaagt ccgccgcgcc tttaccgcac
     7921 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
     7981 acctctccgg cgacaaaacc ctgattgccg cgttccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgtccgag caacgccgct
     8101 atgccaaaac catcaacttc ggcttaattt acggtatggg gcaatacggt ttggcaaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg
     8281 aaaccctgtt cggcagaagg ctctacctgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgccggagc cgaacgcgct gccatcaacg cccccatgca gggcaccgcc tccgacctca
     8401 tcaaacgcgc catgatagac gtgtcccgct ggctttcaga gtgcgaagcc tccccgtggg
     8461 acgaactctt acaaagcaaa ctgattatgc aggtgcatga cgaactggtg ctggaagtcg
     8521 ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg aaagtggacg
     8581 gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat tgggaagagg
     8641 cacattgatt gaaaggtgtt atatgctatc tttatttaaa taaaatttaa tttttggtat
     8701 attttttcta aatgttccta
//
LOCUS       DAWISC010000077         8646 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWISC010000077 DAWISC010000000
VERSION     DAWISC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.077"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(104..1027)
                     /gene="era"
                     /locus_tag="WIW87_001237"
     CDS             complement(104..1027)
                     /gene="era"
                     /locus_tag="WIW87_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ5174400.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1075..1794)
                     /gene="rnc"
                     /locus_tag="WIW87_001238"
     CDS             complement(1075..1794)
                     /gene="rnc"
                     /locus_tag="WIW87_001238"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ5174401.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKRKKK"
     gene            1961..2284
                     /locus_tag="WIW87_001239"
     CDS             1961..2284
                     /locus_tag="WIW87_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ5174402.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2332..2808
                     /locus_tag="WIW87_001240"
     CDS             2332..2808
                     /locus_tag="WIW87_001240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ5174403.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2886..3311
                     /gene="nusB"
                     /locus_tag="WIW87_001241"
     CDS             2886..3311
                     /gene="nusB"
                     /locus_tag="WIW87_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ5174404.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3679..4713)
                     /gene="pyrC"
                     /locus_tag="WIW87_001242"
     CDS             complement(3679..4713)
                     /gene="pyrC"
                     /locus_tag="WIW87_001242"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ5174405.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4929..5075)
                     /locus_tag="WIW87_001243"
     CDS             complement(4929..5075)
                     /locus_tag="WIW87_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174406.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5076..5300)
                     /locus_tag="WIW87_001244"
     CDS             complement(5076..5300)
                     /locus_tag="WIW87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ5174407.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5409..6020
                     /locus_tag="WIW87_001245"
     CDS             5409..6020
                     /locus_tag="WIW87_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ5174408.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6080..6952)
                     /locus_tag="WIW87_001246"
     CDS             complement(6080..6952)
                     /locus_tag="WIW87_001246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ5174409.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEIAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6990..7775)
                     /locus_tag="WIW87_001247"
     CDS             complement(6990..7775)
                     /locus_tag="WIW87_001247"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ5174410.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSWIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            complement(8200..8292)
                     /locus_tag="WIW87_001248"
     tRNA            complement(8200..8292)
                     /locus_tag="WIW87_001248"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8256..8258),aa:Ser,seq:gct)
     gene            complement(8449..8646)
                     /locus_tag="WIW87_001249"
     CDS             complement(8449..8646)
                     /locus_tag="WIW87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174411.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
BASE COUNT         2101 a         2561 c         2051 g         1933 t
ORIGIN      
        1 acagataata tcaacccgtt tttaacaaag atattcccga ttgaaacaaa atgccgtctg
       61 aaacctgttc ggacggcatt tgcgtaaagt tcagcaagaa aaactacaaa cccagctcgc
      121 gcaggaagcg gatgtcgtcc gcccaaccgg atttgacttt gacccagacc ttcaaaaata
      181 ctttggtatc aaacagtttt tccatatcca accgcgcttc ggtggaaatt ttcttcaaac
      241 gttctccgcc tttaccgatt aaaattgcct tttggctttc cttatcgacc aaaacggcga
      301 tatagatgcg gttcaaaccg tcttcctctt caaactgctc cacttcgacg ttcatcgcat
      361 aaggcaattc ctcgcccaaa tagcggaaca atttttcacg cacgatttcc atcgccaaaa
      421 aacgcgccga tttgtccgta accatatctt cgggatacat cggcacgctt tcgggcagat
      481 acggcttaat cagctccaac aggttggcaa tccgcaatcc gtgtttcgcg ctgaccgcct
      541 ccgccgccgc aaattcaaat tcggcgcgca cttgggcaac aaacgcctcc agcgcgtaac
      601 ggtctttcgc cttgtccttg tcgattttgt tgaccactaa aatgaccggc gtgtgcttgg
      661 gcagttgttt caacacgacg cggtcggcat cggtaaagcg catcgcctcc acgacgaaaa
      721 ccaccacatc cacgccgccg agcgcctcgg taacattttg attcagcctg tcgttgagcg
      781 cgttgcggtg gtcggtttga aagccgggcg tatcgacaaa cacgaactgc gcggtatcgt
      841 cggtataaat ccccgttacg cggttgcgcg tcgtctgcgc ctttttgctg gtaatactga
      901 ttttctgacc gatgagatgg ttcatcagcg ttgatttgcc cacgttcgga cggccgacaa
      961 tcgctacgaa gccgcaacgg tatccgccgg cggcgcgttc ccctgcaagg aaggtttcaa
     1021 tatccatatt tgctttcgta tccatatgtt cagacggcat attcacgcgc cgcctcattt
     1081 ctttttcctc ttcagcggca gcttctcttc cagccatttc aaagcctctt tcgccgcttc
     1141 ctgctccgcc gccttgcggc tcgtcccttt ggcacggcac acgaaaccca gttcgcccaa
     1201 atcgcaggaa atgacaaaca tactgtcgtt ggcataaccg atttgctctt cgatacggta
     1261 tttcggcaag gcgaaacggc gcgcctgcaa cgcctcctgc aaagcagttt tgccgtcttt
     1321 tgcctgattt tgaaaatcgg cgcgccggac gcgatcggca aacaaatggc gcaccacctt
     1381 ttccgccgtg ttgaaatcgg catcgaagct gacggcagca aacatcgcct ccatcgcgtc
     1441 tgccagtatc gaaggccgtc tgaagccgcc gctcttcaac tcgcccgccc ccaagtacag
     1501 gccgtcgccg acattcattt ccgccgccat ttccgccagc acgccctcat tgaccagact
     1561 tgcccgcaac cgcgacaact cgccctcggt caacttcgga aacgcgtcaa acagcatccg
     1621 cgccaccgta taattcaaaa tcgaatcacc gacaaactcg aaccgctcgt tgtgcttcgc
     1681 atgatggctc ctgtgcgtca aagcctgccg caaaagcgaa atatcgcgga acgcgtagcc
     1741 cagtttcttc tgtatcgccg cgtgtgcctg ctgtttcaaa acatcgtctt tcattaccgt
     1801 cttccgcaaa aaacagcccg aatgcgtttc catactccaa aaatacagaa acaccttaag
     1861 gctgcaaacg gcgcacattg taccaccaaa ccgccgccgt ctgccgacaa cgcacgaatc
     1921 gtggtaaaat ccgtaagttt tcccccaacg tattcccgta atgaacgcat ttgcacaagc
     1981 actttcctcg gcactcgacc gctgtatcgc caccaacacc gtcgccaaac aaaaccggcc
     2041 cgtcggcttc ctctaccgcg aagcccccgt ttttgaaaac gacagcggct ggcgtttctt
     2101 cagcggcgac gagaccgacg aatacaccga cgatccggac aacttcagca tcgtcagcct
     2161 cgccgacatc gccaaaacca accctgaaac cgccgcactg ctctcccaac ccgaaggcag
     2221 cgcatgggag cttgccgaag acggcacatt ccaaaccgtc gccgactggc agccgcaaga
     2281 ctgaaacctt cagacggcat tccgaaacct ccgaatacca aaggacaccc aatgaacacc
     2341 atcgccccca acctcgacgg caaacacctc cgcatcggca tcgtacaggc acgcttcacc
     2401 aacgaaatcg gcagcgaaat gctcaaagtc tgctgccgca ccctccaaga attgggcgtg
     2461 gcagacgaaa acattaccgt cgccaccgta cccggcgcgc ttgaaatccc catcgcgctg
     2521 atgaactttg cctcttccga aaagtttgac gcactgattg ccatcggcgt cgtcatccgt
     2581 ggcgaaacct accatttcga gctggtttcc aacgaatccg gagcaggcgt cagccgcgtc
     2641 gcactcgact acaatatccc gattgccaat gccgtcctaa ccaccgaaaa cgacgcgcag
     2701 gcaatcgaac ggattgaaga aaaagcctcg gatgccgcca aagtcgccgt cgaatgcgcc
     2761 aacctcgtca acctgctgct cgaagaacag tttgaagacg aagaataaca aacagggcgt
     2821 acaccaatac accctttccg tttcagacgg cataccgtgc cgcccaaccg tttcaaggaa
     2881 aaatcatgaa aacagcccgc cgccgttccc gcgagcttgc cgtacaagcc gtttaccaat
     2941 cccttatcaa ccgcaccgcc gcgcccgaaa ttgctaaaaa catccgcgaa atgcccgact
     3001 ttgccaaggc agacgaagaa ttgttcaaca aacttttctt cggcacgcaa accaatgcgg
     3061 cagagtatat ccgacaaatc cgcccgctac ttgacaggga cgaaaaagac ctcaacccca
     3121 tcgaacgcgc cgttttgctg accgcctgcc acgagctgtc cgccatgccc gaaacgccct
     3181 accccgtcat tatcaacgaa gccatcgaag ttaccaaaac cttcggcggc acggacgggc
     3241 acaaattcgt caacggcatc ctcgacaaac tcgccgccca aatccgcccc gacgagccca
     3301 aacgccgttg atgcatcaca aatttaaaga aagttctatg tccttatttc tgctttcttt
     3361 gtttcgtcat tcccacgcag gtgggaattt ggttttttga attttgggta tttcaaataa
     3421 attctgaaac ttcaatctcg tcattcccgc gcaggcggga atccggtttt ttgagttgct
     3481 gccgtttttg ataaactctg gtcgcatcga atttctggat tcctgccttc gtgggaatgg
     3541 cgaaatttga aaattgtggg aatttatcgg aaataaccga aacgtaacaa actcaaacgc
     3601 cgttgacgta tcaatcagtc agcaacacaa atgccgtctg aaagccctcc cttcagacgg
     3661 cattttgttt ccagcccatc aatactgcac cgtccagccg atttcgccgc ccgcgcgcat
     3721 cgggacaagt tcgccgtcgc cgtagggaac acttgcggga acggtttggc tttgtttgac
     3781 gagggtgatc gtgtcggtat tttcaggaat gccgtagaac cttgcgccgt tttttgaggc
     3841 gaaggcttcg agtttgtcca acgcgcctgc tttttcaaat acttcggcgt aaagctcgat
     3901 agcggtcatc gcactgaaca taccggcgca gccgcaggcg ttttctttgg cggatttggc
     3961 gtgcggcgcg gagtcggtgc cgaggaagaa tttatgcgcc ttctcgccgg taacggcggc
     4021 gaccaatgcc tgacggtggg tttcgcgttt gagtacgggc aggcagaaat gatgggggcg
     4081 cacgccgccg accaagaggt cgttgcggtt gagcaggagg tgttgcgggg taacggtggc
     4141 ggctacgttg tcgcctgctt ccaaaaccag gcgggcggct tcggcggtgg tgatgtgttc
     4201 gaacacgact ttaagattcg gcacttgcgc caaaacgggt ttcatcacgc gcccgataaa
     4261 ggcggcttcg cggtcgaaga tgtcgatttc ggggtcggtt acttcgccgt gaacgaggaa
     4321 caaaatgccc tgtttcgcca tttcttccaa cacggggatg agcttgaaca ggtcggttac
     4381 gccggaatcg gaattggtgg tcgcgccggc agggtagagt ttgaaggcga cgatgccggc
     4441 ggctttggct tcgcgtacaa gttcgggcgt ggcgttatca gtcaaataaa gcgtcatcaa
     4501 cggctcaaac gcgctacctt cgggcaacgc cgccataatg cgcgctttgt aggcaagcgc
     4561 gtcggctaca ctgacgacag gcggtttgag gttgggcata atgacggcgc gccccatttg
     4621 gcgggcggta taaggggcaa cggctttgag cgcgtcgccg tcgcgcaggt gcaggtgcat
     4681 atcgtcgggg cggataatgg tcagggtttg catggttgtt cctttttagg ttttggctgc
     4741 gtttgaatac ccggtcgatt ccatccgttt gccgggggaa agccggttat tccgtccggt
     4801 cggaaaccgg ttccattgga tgaaaggcat tttgtccgac tgattgggtt gcatcaacgg
     4861 aatcttcaaa cacagccttt agattttgat tgtgcttggg ttttgcttct ttgccgacgg
     4921 caaggcggtt aacagccatt ccggaacaaa tcggcggcga tacgcccgaa atgccgacac
     4981 tgcacgccgg acggtttgtt tttgaaacgg cacgcggcac atacgatatt caaaaacaac
     5041 gccgtcgggc gggacatccg catttcagac ggcatttatt tgtgttggac gaccagcgtg
     5101 aacacgccgt cctgttcgga agcatccaac agcacatgac cggtttggcg gcaaaaagcc
     5161 tcaaaatccc ccggtgcgcc gccgtcggtt gccagaacgg tcaacacgtc gccctgctgc
     5221 atttgcgcca aagccttttt cgcccgcaaa atcggcaggg gacatttcaa tccggttacg
     5281 tctaaagttt cgctgttcat cgtttgtacc gtttaaaaaa tgggaatcgg ggggagatta
     5341 taccccagtt gcttgcagcc ccctttgcca acccataaaa taacgccgtt ttcaatttgg
     5401 agaaacttat gcgccgcacc gccctcctgc tgctcgcact cgccgccggc acgtccctcg
     5461 ctgccggctt cagccaaaaa gacacgccga tcaacacccg ctaccgcgaa acgcccgaag
     5521 aggccgccgc ccgcgaattt aaagaacata ccgccaaact gccgccgctg cccgatacgc
     5581 actcggacgg ctggttcgac atttatgtgg atgaaaatta cggcaaacag ccgaaaatcc
     5641 tgctcgacag cctgcaaatt atgcccgcgc ccgacggcag catccgctac attctcaata
     5701 tccgctccga caaaggctac gacaacctga ctgccgaagg catattctgc gcccgcttct
     5761 ccatcaggtt cggcaacggc aaaccttcct cctataaagt gttcggctac ggcgacacgg
     5821 tcaaccgccg ctggatacag ccccgaaacg ccgaatggaa accgataggc ggtacattaa
     5881 gccgcaacga cgcactgcgc accgtcctgt atcaggcatt ctgcgagggc ggtgtacccg
     5941 ccgacacgaa aggtttggta caacgcctga aagaacgtgc cggacgctat gccccgtcga
     6001 taaagccgca caacaaataa ccagacacaa aaataccgtc tgaaacatcg gtttcagacg
     6061 gtattcgatt catcagccat caagcggcgg aaactttgtc ctgacggcac aacagcgtaa
     6121 tcaaatcact gatgcgccgc ttcatatcgc ggcggtcgac aatctggtcg attgcgcctt
     6181 tttccagcaa aaactcggcg cgttggaagc cttccggcag cgtttcgcgc accgtctgct
     6241 caatcacgcg cggaccggca aagccgatta atgcattggg ttcggcaagc acgacatcgc
     6301 ccaaaaatgc gaagctggcg gatacgccgc ccatagtcgg atcggtcaac accgatataa
     6361 atggcaggcg tttttccgtc agcaaatgca gcgcggcact ggttttcgtc atctgcatca
     6421 gcgagtttac accctcctgc atacgcgcac cgccggaagc cgccacacag acgaacggac
     6481 aattgtcggc aaccgcccga cggatacctt gtacgaatcg ttcgcccaca accgaaccca
     6541 tcgaaccgcc gataaagcgg aattcaaacg cggcaacgac gacgggcaga ccgttcatct
     6601 tgcctttcat taccaccaaa gcatcatctt ccccggtcag cttgcgtgcc gcactcaaac
     6661 gatccggata ttttttgctg tctttaaact tcaaaggatc ggtcggtttg acatttccgg
     6721 caatctcctc cctgccttcc tcatccaaaa gcagattcag gcgttgtcgt gccgacaacg
     6781 gattgtggtg gttgcatttc ggacaaacct gattgttctg ctgcaactcg gtcgaataaa
     6841 cggttgccga acaagacgga catttgtgcc acaaaccctc gggaacattg gaagaaccgt
     6901 ctttgctgcg gtttttgatt ttgggtggca ggattttatc taaccagctc atggacaact
     6961 cctaaaatat tcaggatgag gaaccgccgt cagcgcacgg catccttcaa ctctttgacc
     7021 aaagcaccga cggccgcagc ctcgttgccc gcattgtttt caatctcttt cacaatccag
     7081 ctgccgacaa tgaccgcgtc ggcaacccgg ccgattttgc gcgcgctttc cgcattgctg
     7141 atgccgaaac cgacaccgat gggaatatcg atatactgat gcaaatactc tattttacgc
     7201 gaaacctcat ccgtatccaa acttgccgcg cccgttacgc ccttgagcga aacataatag
     7261 acaaatccgc ctgccagctc ggcaatggtt ttaatacggt cttccgtcgt cgtcggcgca
     7321 atcaggaaaa tacagtcgac cccgttatcc ttcagctcgc gatagagcgg atcgatggtt
     7381 tcgacagggg aatccaccgt caacacgccg tccacacccg cctttgcggc ttcctgagca
     7441 aactcccgat aacccatctt atgtacaggg ttcaaatatc ccatcaaaac aaccggcgtt
     7501 tgcgtgtcgg tttcacggaa ttttctgacg acatccaaga catcgcgcag cgaaatcccg
     7561 tttgccaacg cccgctccgc cgcacgctga ataaccggcc catccgccat cggatcggaa
     7621 aacggcacac ccaactccaa aatatccgca ccgtttgcaa ccatgccgtg catcaatgcc
     7681 aaagttgtcc gaatatcggg gtcgcccacc gtaatatagg gaatcaatgc ctttccgcca
     7741 tccaaagcgg caaaagcctg ccggattctg ctcatttgtc tcttccatcc tatcggcaaa
     7801 tatgccataa aacacaatca gtaaattttt cccagtataa catttgcggt acggataggc
     7861 gaatacggca aacagaaacc acgcaaatca gagccgccgt ttgaacgagg cggaaataaa
     7921 cggcaagcat ctcggattat agtggattaa caaaaatcag gacaaggcgg cgaagccgca
     7981 gacagtacaa atagtacgga gccgattcac ttggtgctcc agcaccttag agaatcgttc
     8041 tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta taacaaaatt
     8101 cagaatcaag acagggcaat accatactgg ttttgcacca acccgctata aaattgcata
     8161 aaaaatagac gctgtaaaac acagcgtcta ttttttatct ggcggagtaa gagggattcg
     8221 aaccctcgat gcaggttgac cccacatgct tccttagcag ggaagtgcct tcagcctctc
     8281 agccattact cctaaggaag ctgtaactat aatcaacttt tccgataccg tcaaggaaaa
     8341 atggaaagca aatattaatc ttttgaaata aaataagatt taccctgatt atacccaatc
     8401 aggcacttct tttcttcctg aatggaaata ttcctgactg ccacatcctc aagcaaaatg
     8461 cttttgtgca tcctgtttga tttcctgaaa ccacgcgtct tcaggaaaac gtgcaagcgc
     8521 ggcatccaat gttgacagta ggttttgcga cagggctttg gtcaggtatt cttcctcgtt
     8581 ggcttctgcc gctttgtcgg gctgttgtgc caaaccgtcg gcacgggctt tgtgtccgtg
     8641 tgccaa
//
LOCUS       DAWISC010000078         8568 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWISC010000078 DAWISC010000000
VERSION     DAWISC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.078"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WIW87_001250"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WIW87_001250"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ5174412.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WIW87_001251"
     CDS             1160..2290
                     /locus_tag="WIW87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ5174413.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFIEGLLGRLLDPQDPTARALLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRIAALEAQFKNALLNASPDFQDEYGYEKDAPGEANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2361..3212
                     /locus_tag="WIW87_001252"
     CDS             2361..3212
                     /locus_tag="WIW87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ5174414.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIRLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFTVIWLFSKKQ
                     RSTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WIW87_001253"
     CDS             complement(3295..3957)
                     /locus_tag="WIW87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ5174415.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..5845)
                     /locus_tag="WIW87_001254"
     CDS             complement(4052..5845)
                     /locus_tag="WIW87_001254"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ5174416.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6090..7199
                     /gene="proB"
                     /locus_tag="WIW87_001255"
     CDS             6090..7199
                     /gene="proB"
                     /locus_tag="WIW87_001255"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ5174417.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7212..8474
                     /locus_tag="WIW87_001256"
     CDS             7212..8474
                     /locus_tag="WIW87_001256"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ5174418.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAATADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPNPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPIRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1919 a         2430 c         2248 g         1971 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccag acaaacaggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaacctgcc
      181 gtcgatgata tgcgtggctt tcacaccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catactccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccgggagaaa ccatggcgga
     1681 atggtttatc gaggggctgc tcggcagatt actcgacccg caagacccta ccgcccgcgc
     1741 cttgctcgac cgcgccacgt tctatatcgt ccccaatatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggcgc gaacctcaac cgcgagtggg aaaacccgac
     1861 ggtagaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggagtgga
     1921 tttgtttttg gacatccacg gcgacgaagg gctgcccttt gtctttgtcg caggtacgga
     1981 aggcgtgccg aactacaatc cgcgcatcgc cgcgttggaa gcgcagttta aaaatgccct
     2041 tttaaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgccgggcga
     2101 agcgaatatg accttggcga ccaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gctggaaatg ccgttcaaag acaatgccaa tctgcccgac gacgacttcg gctggaacgg
     2221 tcagcgttct ttacggctgg gcgaagccgc gttgtccgct atcctcaacg tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgatcccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatacgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggatacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcaccgtc atttggctgt
     3001 tctctaaaaa acagcggtcg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gtatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggtttgttg atgactgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgccc tgcgctttcg
     4081 gtttggcggc actaaattcc agcttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 aaccggttgc ggaagcgcgt ttttcttcac ctttgatgct gaacacgatg tcggcgcgcg
     4381 gttcttctcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgtgcaaatg ccgcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttatacg ggatggaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5521 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5581 tgataacgcg gttggtctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5641 aagcattcaa atccagcggc ctgccttgcg cttccgccaa cgcggtcagc ttttgcagtt
     5701 gcgccagagg cagcgagccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     5761 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     5821 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     5881 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaatct
     5941 caataagccg ccatagattt tattaatttt atacattttg tccattttat tgcagacgcg
     6001 gcgtgattgt tgacaattgc ggcagaacag ggcaaattcg gcggcacaag gtggcaacga
     6061 gacaggacaa accaacaagg acggaaacaa tgaaatacaa aagaatcgta tttaaagtcg
     6121 gcacatcttc gattacccat tcggacggca gtctctcgcg cggcaaaatc caaaccatca
     6181 cctgccagct tgccgcattg catcatgcgg gacacgagct ggtcttggtg tcttccggcg
     6241 cggttgcggc agggttcggt gcgctgggtt tcaaaaaacg tccggtcaaa atcgccgaca
     6301 aacaggcttc cgccgccgtc gggcaggggc tgctgatgga agaatatacg gcaaacctgt
     6361 cttcagacgg catcgtgtcc gcgcaaatcc tgctcagccg cgccgacttt gccgacaaac
     6421 gccgctacca aaatgccggc ggcgcacttt ccgtgctgct gcaacgccgc gccgtcccca
     6481 tcatcaatga aaacgatacg gtttcggttg aggaattgaa aatcggcgac aacgacacat
     6541 tgagtgcgca agtggcggcg atgatacagg cagacctctt ggtgctgctg accgacatag
     6601 acggtcttta cacgggcaac ccgaacagca atcccgatgc cgtacggctg gacaaaatcg
     6661 aacacatcaa ccatgaaatc atcgaaatgg cgggcggctc gggttcggca aacggcacgg
     6721 gcggtatgct gaccaaaatc aaagcggcaa ccatcgccgc cgaatccggc gtaccggtgt
     6781 atatctgttc ctcgctcaaa cccgatgcac ttgccgaagc tgccgaacat caggcggacg
     6841 gctcgttttt cgtcccccgt gccaaaggtt tgcggacgca gaagcaatgg ctggcgttct
     6901 attccgaaag ccggggcagc gtttatgtgg acgaaggtgc ggaacacgct ttgtccgaac
     6961 aggggaaaag cctgctgatg tcgggcattg ccggaatcga agggcatttt tcccgtatgg
     7021 acaccgtaac cgtgtacagc aaggcaacca aacagcccct gggcaaaggg cgcgtcctgt
     7081 tcggctctgc cgccgccgaa gacctgctca aatcgcgtaa ggcgaaaggc gtgttcatcc
     7141 atcgggacga ctggatttcc atcacgcccg aaatacgcct gcttctgacc gaattttaga
     7201 aaaggaaacc catgtcaaac acacaaaaac agcttgccct tgccaaagcg gcaaaaaaat
     7261 ccgtcaacac ggcggatacg gaagaaaaaa accgcgcgct gcttgcgatg gcggacagct
     7321 tggaagcggc gacggcagat attttggcgg caaaccgtca agacttggaa gccgcggcag
     7381 gcaacattcc cgaaagcatg accgaccgcc ttttgttgga cggcaaacgc atttgcgcga
     7441 tggcggacgg catcagggcg gttgccgcgc tgcccaaccc cgtgggcgaa atactggaaa
     7501 cctcgacttt gcccaacggc ttggaaatcg tcaaaaagcg cgtggcgatg ggcgttatcg
     7561 gcattattta cgaaagccgc ccgaacgtta cttccgatgc ggcggctttg gcactgaaaa
     7621 gcggcagcgc ggtcgtactc cgcagcggca aagatgcatt ccaatccgca cgcgccatcg
     7681 ttgccgccct gaaaacgggg ttggcgcaaa cccgcatcga ccccgacgcg ctccaactga
     7741 ttgaagacac cgggcgcgaa agcagttacg aaatgatgag ggcgaaagat tatctagacc
     7801 tgctgattcc gcgcggcggg gcggggctga tacgggcggt ggttgaaaat gccgtcgtgc
     7861 cggtcattga aacgggaacg ggcattgtcc acatttatat cgacaaggac gcggattggg
     7921 acaaggcgct ccgtatcgtg tacaacgcca aaaccagccg tccgtccgtg tgcaactcga
     7981 tggaagtgct gctggtacat gaagacattg ctgccgactt cctgcccaag ctcgaacggc
     8041 tgttggttcg cgaccgcata gaagccggac tgccgcccat ccgcttccgt ttggatccgc
     8101 aggcggcgcg gcatatcggc ggcgaagcgg cgggtgcaga cgatttcgat accgagtttt
     8161 tagactacat cctcgctgtg aaaaccgtcg cttcggtcga agaggcggtc tggcacatcg
     8221 aaacccacag cacacaccat tcagacggca tcgttaccga aaaccgccac gctgcggact
     8281 atttcacgac tcatatcgat tctgccgccg tgtatgtcaa cgcgtccacg cgctttaccg
     8341 acggcggcga attcggcttg ggttgcgaaa tgggcatctc cacgcaaaaa ctccacgccc
     8401 ggggcccgat gggtttgaaa gagctgacga gttacaaata catcgtacaa ggcacgggac
     8461 aggtcaggga ataatccgaa ttcaaatgtc tgatatagtg gattaacttt aaaccggtac
     8521 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctga
//
LOCUS       DAWISC010000079         8567 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWISC010000079 DAWISC010000000
VERSION     DAWISC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.079"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WIW87_001257"
                     /pseudo
     CDS             complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WIW87_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
     gene            complement(3125..3484)
                     /locus_tag="WIW87_001258"
     CDS             complement(3125..3484)
                     /locus_tag="WIW87_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174419.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRASLMAMLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLVGGFPLSLPLVVLLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3432..3662
                     /locus_tag="WIW87_001259"
     CDS             3432..3662
                     /locus_tag="WIW87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ5174420.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3655..4296)
                     /locus_tag="WIW87_001260"
     CDS             complement(3655..4296)
                     /locus_tag="WIW87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ5174421.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4286..4837)
                     /locus_tag="WIW87_001261"
     CDS             complement(4286..4837)
                     /locus_tag="WIW87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ5174422.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIDRLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4841..5359)
                     /locus_tag="WIW87_001262"
     CDS             complement(4841..5359)
                     /locus_tag="WIW87_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ5174423.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5359..6999)
                     /gene="pqiB"
                     /locus_tag="WIW87_001263"
     CDS             complement(5359..6999)
                     /gene="pqiB"
                     /locus_tag="WIW87_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ5174424.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(6992..8452)
                     /locus_tag="WIW87_001264"
     CDS             complement(6992..8452)
                     /locus_tag="WIW87_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ5174425.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPQLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1916 a         2200 c         2417 g         2034 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtctgcccg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaaccca tttgttttaa agttatagtc gagatacgtg tatcggtgca accagtctgc
      181 cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtcttcgg ggtttcaacg
      241 gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg tcgccgtaca
      301 cgtcgtccac acggttgacg ctcggccaga atttgtgttc gcggacgaac ggcaatggga
      361 agacggcttc ttcgcgggaa tacggatgcg cccagttgcc ggttatatct gcggcggtgt
      421 gcggcgcgtt gaccagtggg ttgtcgtctt tcggccattc gccgcgcccg actttcagca
      481 cttcctgttt gatttgtttc agggcggcga tgaagcggtc gagttcggct ttgctttcgc
      541 tctcggtcgg ctcgatcatc agcgtaccgg caacggggaa ggaaaccgtc ggggcgtgga
      601 agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt tcggctttga
      661 gcggacgcaa gtcgacgata cattcgtgcg cgacgcggcc gtttttgccg gtgtagagaa
      721 tcggataatc ctcgcccaag gctttggcga cgtagttggc gttgagcaat gcccagcgcg
      781 ttgcctgttc catgccttgt ttgcccatca tggtcaggta catccaagta atcggcagga
      841 tggatgcaga accatatgct gcggcagcca cggcggtttg cccggcactt gtgctgtgcg
      901 tgtcggtcaa agcgtgtccc ggtgcaaacg gggcaaggtg ggctttcaag ccaatcggac
      961 ccatgcccgg gccgccgccg ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca
     1021 acacgtccgc gccgacttcg gcaggctgca tgatgccgat ttgagcgttg aggttggcgc
     1081 cgtccatata aacctgtccg ccgttttcat ggatgatgcg gcagatgtcg cggatgcctt
     1141 cttcatacac gccgtgggtg gacggatagg taatcatgat ggcggacaaa gcgtcgcggt
     1201 attgctcggc tttggctttc aaatcgtcga tgttgacgtt gccgtgttcg tcggtatcga
     1261 cgacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg tgggcggatt
     1321 tgggaatcag gcagatgttg cggtgcgctt cgccttgggc ttcctgatag cggcggatgg
     1381 caagcatacc gctgtattcg ccctgcgcgc cggagttggg ctggaaggaa attgcatcaa
     1441 aaccggtgat ggatttcagg ctgttttcca tatcggcgag caattcgcgg tagccggcgg
     1501 tttgcgcttc aggagcgtaa ggatggatgt cggagaactc cgcccaagta atcggcaaca
     1561 tttccgcagt cgcgttgagt ttcatggtgc agctgcccaa tgaaatcatg ctgcggttca
     1621 tcgccaaatc gcggtcttcg agttttttca gatagcgcaa catttcgtgt tcggtgtggt
     1681 aacggttgaa cacaggatgt tgcagaatgt catcttgacg cagcaattcg gcgttcagac
     1741 ggcctttgac atcatcggca aatgcgaacg catccttgcc ggtaaacgcg cggtacaaat
     1801 caaccaaatc ttcgtatgcc gatgtttcat ggaatgcagc cgcaacttga gtatcgttga
     1861 cgcggcgcag gttgtaaccc gactctaaag cagcggcaaa cacttggtct gctttctctt
     1921 tgttgccgaa atcgacggta acggtatcga agaagacttc gtgaaccact ttgatgccgt
     1981 ctgaaaccag tgcatcggca aaggcagaag ccagcgcgtg aatgcggttg gcaatgcgtt
     2041 tcacaccttc aggaccgtgg taaacggcat acataccggc caagttcgcc agcaatgcct
     2101 gcgcggtaca aatattggat gtcgcttttt cgcggcggat gtgttgctcg cgggtggaca
     2161 acgccatgcg caaggcaggt ttgcccgatg cgtctttgga tacaccgata atacggcccg
     2221 gggcggaacg tttgaacgcg tctttaaacg cgaaataagc ggcatgcggc ccgccgaagc
     2281 ccatcggtac gccgaagcgt tgggtgttgc ccaacgcaat gtccgcgccc aattcagccg
     2341 gagacttcag caaaaccaaa ctcatgatgt cggtggcaac gaccacgatc gtgccttttg
     2401 ctttcagacg gctgataaca tcctgcaaat cttgtacgtc gccgtctttg ccgacgtatt
     2461 ggaacagcgc gccgaagtat tcgccttcat ccgctttgac aaaatcgccg accaccagtt
     2521 caaagccgaa atatttggcg cgggttttca tcacgtccaa agtttgcgga tacacgcgct
     2581 cgtccacaaa gaaacgcccg gatttcacct tgcccacgcg gtgcgccatc gccatcgctt
     2641 cggcggcggc ggtcgcttcg tccagcaaag acgcgcccgc cacagggaaa ccggtcaaat
     2701 cgatacacac ttgttggaag ttcaacaaag cttccaaacg accctaggca atttccgcct
     2761 gatacggcgt gtaggcggta taccagcccg gattttccaa tacgttgcgc aaaatcacat
     2821 tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga cttgttgatc acgtttttcg
     2881 acgcaatgcc tttcaatttt gccaacgcgt ccgcttcggt cagggcatcg ggcaaatcga
     2941 gttcggacgg catacggatg ctttgcggca cggtattgcc gacaaaatcg tccatacttt
     3001 tctcgccgac agcggcaagc aacgccgctt cgtcgccgaa actcaaatgc cgcgcggcaa
     3061 attcgttggg gttgaacagt tcggacagtt tcatttctac tccttcgggt ttgtagggct
     3121 gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac aaaagacggg
     3181 ataatggcgg acaatgcgcc agaatgcagc aggtcaggac aagcagcacc acaagcggca
     3241 gcgataaggg aaagccgccg accaaaacca tgaaacctgc aggcaccagc caagccaagg
     3301 gatacgccgg aagcggaaaa acaatattga gcagaaccaa aatcacgaca aacagcggca
     3361 gcatcgccat caggctggca cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt
     3421 cgcaatattc cataatttca gcctgtccgg gctttatcca cagcaggttc ggacggtcgg
     3481 tcatgccgtc tgaaccggca ggccgtcttt ttcaaaaaac gccataccgc tgcaacttca
     3541 tgcggctgta tggcgtaggt tttgcacttc agacggcata tgcgtcccga agcagtttgg
     3601 tgtcccagtc ggttcatgat tatataaaca acttgggtgc ggataaacct ttgtttatct
     3661 aaacccctgt tttctcaaaa attgattcaa atcttcggca aatttccgat agcctttgcc
     3721 gttggcgtgg atttggtcgg atttcagatt attatcgccc aaaatttccg cccacgcgcc
     3781 gccgaacagc ggaatgccgt attcctcgga caaatcctca tacagcggat gatcgctcaa
     3841 atgcccgaac aacgcaccca gtgtgatgtg cggcacgccg acgaggacgg cggggatgtt
     3901 ttccttctgc acggtttcga tgattttcgc gatattggcg cgggtctgct ccttgggaac
     3961 tttgcgcaga aagtcgttgc cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa
     4021 cagcgcgggc aggcgcgaca gggcttgggc ggatgtatcg cccgatacgc cgccgttgac
     4081 aatattccaa cccgtcagtt tttgcagttg cgcggggtag gattcgccgg ggtttgcgcc
     4141 gtagccgaag gtaagcgaat cgcccaaggc aagtacggtg cttccttcgg gaattttggc
     4201 gtgggttcgg gcggattttc tgccgcaggc ggtaaggagc aacgcgcctg cgccgaggag
     4261 gaaggttctt ctgttcattg gtttttcaga cggcatcgag ccatttcggg tggtatttca
     4321 gggttttacg gtaaaccgga cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca
     4381 tcagaaattc gccgacgatt tcgccgccga caaatttgaa atgtttttta aagagtttga
     4441 cccattcgtc tttgcttcgc ggatggtgcg tgtcgagcca gttcttgaac gagccgtatt
     4501 cttgttgcaa cgcttggatt tgccgtgcat tgaaaatggc ggcatcgatt ttcaggcggt
     4561 tgcggacaat gcccgcatcg gcaagtaacc gatcaatatc ggcttcgtca aatgcggcga
     4621 ccgtatcgat gtcgaaccct tcaaatgctg tctgaaacgc ctgccgcttc ttcagcatca
     4681 gcgtccagct caatcctgcc tgattgattt ccaacaccag ccgttcaaac aattcattgt
     4741 cgtcctcaat cggaaaaccg tattgcgtgt cgtggtaatg tttgttcggg ttgtcggtgt
     4801 tttcgggaag tgaggcggca aattcgcaat agttcacggt ttactcgacc atctgttgcg
     4861 ccgcctgttt cagtccctgt tcgagtgcgg cggtcatcgc ggcgtagccg tcaccctgct
     4921 gttcggtttc gatatggaag ggtctgttcg taccgtcggg taggacggcg tagccgctga
     4981 tgagggtttt gcccgtgtag ctgccttgga atgcgtcaat atagaccgtc catttttcgg
     5041 tactgccgct gcgtgaggca ggaacaaaga tgcgtgtgct gtccaaacgg ttgaatgcat
     5101 tgctcaacgc cgcttcgagc atatcgtcca aggtgtccgc ccaaacgtgg ttttgtgcgg
     5161 tgttgaggcg gtaggggtcg gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa
     5221 ggcggacttc gacggcggtt tcgccgcctt gcgttgcagg gcggatgtag cggctgtcgg
     5281 gcaacacgaa atattgtgtg ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg
     5341 cgatggggaa aaggcgcatt atcggcttcc tttcgggata gggtctttgc tgctgctgtt
     5401 gaaaatcagc gcgttgggtt tttctttcaa agtgttaatg acgggttgaa cgtcttttaa
     5461 agttttgtcc aaactttgca gcgtattttg tacgtcgccg tagataggcg attgcggcga
     5521 tacgccttgc agggttgtgc gcaactcttt cagggtttgg ttcagctcgt tcggaatgtt
     5581 ttgcgtttgc ggattattgc ttagcgtgtt aatggatttc agcgtggatt tgagttcggc
     5641 gagcgaagcg tttaaacctg aaacggtttt gtctaaaggc agtttgccga atttgtccag
     5701 caaatccgcc aatttgacct gtaagtcgtc caaaccgccg ccctgggtcg cgataacggt
     5761 atcgcctgca taaacggtat gcggtcgcag cttgggcgaa gacgaaggct gatcgttcaa
     5821 ctcaatcatt ttgctgccgg tcagcaggtt gttgctggag atggtggcgg tcaggccttt
     5881 gtttaaagcc gtctgaaatt gttgtttcca atgctctttg ctttgttcgt cggcattgat
     5941 ttccaaacgg gaaggctcga tgcggatgcg tacgggtatc cagccgtttt caaataaatg
     6001 caggctgtcg ttgcggtcga aataagggac atcggaaacc acgccgacat tcagcccttt
     6061 gtactcgacg ggcgaaccga cggtcaggcc gcgcacggat tgtttgaaaa acgcggtgta
     6121 gtacagcgag cggtcgtcag gcaggttggc gacttcgctg cggctgtcgt aaagcgtgaa
     6181 gctgtcttcg cttttgacgt ttttactgtt tttggttttc ggcgaatcaa atgaaatcgc
     6241 gcccgacagc agggcaggca gaggggcgga attgagtttg atgccgctgc ctgtggtttc
     6301 gatattgatg ccgctttcca gccaaaaacg gctggcggaa tgaatcagtt tgtcgttggg
     6361 gctttggatg aagatggtgt aatgcacgct ttggtcggac gggtcgaaat gcgcgctttc
     6421 gacttgcccg accataaagt tttcatacaa aacagggctg ttgacgttga ggatgcggtc
     6481 gtttttacca atcaaattca agcgcagccc gctttgcccg atggcggtaa cgggcggaat
     6541 gtcctgcacc tggaacacgt cttttgcctc gtcgcttttg ccgggtgtaa aggcgatgta
     6601 cgaacccgaa agcagcgtac ccaaaccggt tacgccgctt tggtcgatac gcggcttgac
     6661 cacccaaaac tgtgtgtcgc tgcggatgag gccggatacg tccgcattga gttgggcggt
     6721 tacttccacg cctttttggt cgtcgcgcag tttgattcgg gtaacgcgtc cgacatcgat
     6781 gctcaatact ttgatgaccg tattgttgac ctcaatgcct tccgcgctgt ccatcaagag
     6841 cgtaaccaca ggccccctgt tgcggatttc cttaacccaa agccagccgc cggcaatcag
     6901 cgcgatcagc ggaaccagcc agacggcaga gaggaaggtg ttgtttttgc ggacgcgtgc
     6961 ttgggcgtgt ccgtttggag gagggctgtt gtcagtcatg tttttccgtt tcattgaaag
     7021 caatgccgtc tgaagcgcgt ttgtcccaaa gcaggcgcgg gtcgaaataa taggcggaca
     7081 gcatcgtcag aatcacgacc aggcagaaat agactgccgc actgcccgga atgacgcgcg
     7141 cggcataagt gtggaacgaa cacatcaaaa taataatcac aaaaatatca atcatcgacc
     7201 agcggccgac cgcttcggtg atgcggtaga ggtgcgacaa tttctttgca cccgttggca
     7261 aagcgaagcg ggcggacgca atcaaaaccg acattgccgc aatcttcagt accggcacca
     7321 aaatactcgc gctgaaaata accgccgcaa tcagcctgtc gccctcgtcc cacatataag
     7381 cgatgccgtt aaggatggta ttgacctccg tggcggcagg attggacgaa atcataatcg
     7441 gcaggatatt ggcagggaaa tacaaaataa ccgccgccgt cagaaacgcc gacgaaatac
     7501 tcagactttt cggccgtcgg cggtacagtt ccgcaccgca cacgccgcag ggggattcgg
     7561 cactgtcgcg gaaatacagg cagcggctgc aacaggtttt accttccgat gccgtctgaa
     7621 ccgcattatc ccccgtcagc cgcccgattt gaaaatacac ccaatgctgg ggaaccgata
     7681 ccgaagtccg aatcagcata accgacagcg cgaacatcag ataaaacgcc gatccgaagc
     7741 gaacctctgc cacagacgag agcttgatat acgccaccaa agtggaaaca aaaaacacat
     7801 ccaccatcat cgcctgtctc aagcgcacca tcacacgcgt tgccaaacgc agcgcaggat
     7861 acgcctgttt ccgtatcagc gcggcataga catacaggca cagcagcaga aacagaaccg
     7921 gcgcgccgaa ggtcagcaca aacatcactt cggccaaaaa accataatcc tgaaacacca
     7981 tcaggcgcat catctcgggc agcgaaagga cggatgccgc acccggtatc ccgacctcga
     8041 tatacgtcat accgtaagca aacgccatca aaatcagcga agccgccgca taagcgggcg
     8101 gggcggaaaa aggatgcctg cccaccctga agagcttata gccgcagcgc gggcagaacg
     8161 ccgcctcccc cctgtccaat tgcggcacat ccgcgcggca gccgcattcc gggcaatcta
     8221 ccgtatgcgg cggcagggct tcgtcgcggc agttgccgcg aaggctgtgg gggatatttt
     8281 cggcaaacgg cttcatcggg aaagtggcaa atataagtgg cggcattata aaggaaaaaa
     8341 ggcgatacac ccaatcagga tgcaaaaccg gcaggttcgg cagtatgaac aaacccttgg
     8401 tgtccggcaa aaatattttt tcgagcggct gtccgccgac tgccgaaccc atttgataaa
     8461 atcccgattc tgtaaagaat agtaaaatac ctgcttaaaa acatcacgca aatgatattt
     8521 tttccaaaaa tatgtgataa taaatttcgt ttatgattct ttattat
//
LOCUS       DAWISC010000080         8431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWISC010000080 DAWISC010000000
VERSION     DAWISC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.080"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            31..168
                     /locus_tag="WIW87_001265"
     CDS             31..168
                     /locus_tag="WIW87_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-like fold family protein"
                     /protein_id="HEZ5174426.1"
                     /translation="MHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVF
                     G"
     gene            complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WIW87_001266"
     CDS             complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WIW87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ5174427.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1841..2224)
                     /locus_tag="WIW87_001267"
     CDS             complement(1841..2224)
                     /locus_tag="WIW87_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ5174428.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2228..3769)
                     /locus_tag="WIW87_001268"
     CDS             complement(2228..3769)
                     /locus_tag="WIW87_001268"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ5174429.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAVPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4059..4892
                     /gene="serB"
                     /locus_tag="WIW87_001269"
     CDS             4059..4892
                     /gene="serB"
                     /locus_tag="WIW87_001269"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ5174430.1"
                     /translation="MPHALVLQFPSAAALPSDFPLRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAVGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4956..6179
                     /locus_tag="WIW87_001270"
     CDS             4956..6179
                     /locus_tag="WIW87_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ5174431.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     NTSLLWFSAVIGPILGVAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVISVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLGLTAVKWLVVLMALAVGAYGGLITPSMMLGSTIA
                     FAAATAWNSVFPEMSSESAAIVGAAVFLGVSLKMPLTAIAFILELTYAPVALLMPLCT
                     GMAGAVWVAKKMGFK"
     gene            6343..7923
                     /gene="purH"
                     /locus_tag="WIW87_001271"
     CDS             6343..7923
                     /gene="purH"
                     /locus_tag="WIW87_001271"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ5174432.1"
                     /translation="MSSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGTPEIAGFPGRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DVPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGATVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1905 a         2284 c         2347 g         1895 t
ORIGIN      
        1 tttgaaggca aaggcgttga tgttttgtat atgcacttcc acaaagccaa cgagtttttg
       61 ggtatgaccc gcctgccgac attcttatgt aacgatgtgg ttaaaaatcc gcaagtggaa
      121 aaatacttgg cagattatca ggcacacttg gaaaaagtgt tcggctaatt aaaaatccat
      181 cttcaacacg gagatggatt ttgtttgttt cgttgatttt gtgtcagttt cagatgtagg
      241 tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat gcaaaaaaat cactgcaaac
      301 cttcataaac ggggtttgca gtgatttttt caaatcaaac agattgaaaa cctgcgccga
      361 attgttcaga cggcattatt ttttcagttc ggacagaatg tcatctacgg ttttcttcgc
      421 atctccgaaa cacatcacgc tgttttcgtt gaagaacagt gggttttgta cacctgcgta
      481 gccggtattc atcgagcgtt tgaagacgac gacttctttt gccttccaca cttccaacac
      541 gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg gcggggttga cggtgtcgtt
      601 cgcaccgatg accaagacca catcggtttc ggggaagtcg tcgttgattt cgtccatttc
      661 caaaacgatg tcgtagggga ctttggcttc ggcgagcagt acgttcatat gaccgggcag
      721 gcggccggcg acggggtgga tgccgaagcg tacttcggtg ccgtttttac gtaaaagctc
      781 ggtgatttcg gcaacggggt attgcgcttg tgcgactgcc ataccgtagc ccggggtaat
      841 gatgacattg tttgcgcctt tcagcatttc ggcaatatcg gcagctttga cttctcggta
      901 ttcccctatc tcttggctgc cggaagataa tgtgccgctg tcgctgccga aaccaccggc
      961 aattaccgag acaaacgagc ggttcatggc tttgcacata atgtaggaca gaatcgcgcc
     1021 gcttgagccg accagcgcgc cggtaacgat gagcaggtcg ttggagagca tgaagcctgc
     1081 cgctgcggcc gcccagccgg agtaggagtt gagcatggac acgaccacgg gcatatctgc
     1141 gccgccgatg gaggcaacca agtgccagcc gaatgcgagg gcaatcaggg tcataatcag
     1201 caggatgaag ccgctgccgt caatgccgac aaatacgagc agcaacacaa acgatacggc
     1261 aagtgccagt gcgttgagct tgtgtttggc gggcagttgc agcgggctgc tgctgatttt
     1321 gccgttgagt ttgccgaatg cgaccagcga gccggtaaag gttaccgcgc cgatgaagat
     1381 gcctaaatac acttcgacca gatggatggt gtgcatatcg tgcgaaacgt tgcccggcgc
     1441 gatatagctg ttgaagccga ccaaaaccgc cgctaggccg acgaagctgt gcagcagggc
     1501 aatcagttcg ggcatttcgg tcatttccac ctttttggct ttgtagatgc cgattgccgc
     1561 gccgatgagc atggcgatga tgatccagcc cagtccgtgg gtattgtcgg aaaaaacagt
     1621 tacaaaaagg gcgaccgcca taccggcgat accggaatag cagccctgtt tggcggtttc
     1681 ctgtttggac agccccgcca gtgagaagat gaataaaatt gcggcaacga tatacgccgc
     1741 tgttacgagt cctgaagaca tagaaattct ccgattttcg atgatttgtt ttcaatgccg
     1801 tctgaaaaat tgacgttcgt gttttcagac ggcatctgtt tcaagcagcc gcgacaaaca
     1861 gcccgacggt gcaggcaaat ccgcctgccc acatcagtga gcgcatagcg gctttgtcgg
     1921 cgatatagca ccagataaag gcgaggcgga acaggatgaa caggcaggca agcgtgttga
     1981 tggtcgattg cgccgcattg ccggttgcgt gtgccgtcaa aacggcggcg gcaaacggtg
     2041 caaaggcttc aaaaccgttt tgctgtgcgg cgtgggcacg ggcggctgcg ccttgcgtgt
     2101 gcgccagaaa accgcgcgga ttgtggttgt ctttaaaccg gaatccgccc gcttttttgg
     2161 catacgccgc acaaaaaagc ggcaataggc aggcaatcag aatacaccaa taggcgaaag
     2221 tcatggctta ccctttctta aacatattca gcatacgccg tgttaccgca aagccgccga
     2281 agatgttgat gccggcaatc aggatggcaa caaacgacag cagcgaaacg aagccgttgc
     2341 cctgaccgat ttgcagcagc gcgccgacga cgatgatgcc ggagatggcg ttggttaccg
     2401 acatcagcgg tgtgtgcagc gagtggctga cgttccagac gacgtagtag ccgatgacgc
     2461 aggcgagaac gaacacgata aagtggttca ggaatgctgc gggtgcgacc gcgccgaccc
     2521 acagtaccaa gacggcggcg atgacggcgg gcgcgagttt tttccacagg ggaacgggtt
     2581 ttggctcggg cttggcggca ggcacggctt tttcagacgg cgtttgctgc ggctgggcgg
     2641 aaacttgaat cggcggaggc gggaaggtga tttcgccgtc gtgggtaacg gtcatgttgc
     2701 ggataatcac gtcttcgaag tccaacgtga tttcgccgtc tttgttcggg cttaacagct
     2761 tggtcaggtt gaccaagttg gtggcgtaaa gctgggaaga ctgtccggca aggcggtttg
     2821 ccatgtcggt gtagccgatg attttcacgc cgttgccggt tacggacaat tcgcccgggc
     2881 gggtgagttc gcagttgccg cccgtcgccg ccgccaaatc gacgatgacg gagccggatt
     2941 tcatgctttc caccatttct ttggtaatca gcttgggcgc gggtttgccc ggaatggcgg
     3001 cggtggtgat gatgatgtcc acttctttcg cctgctcggc aaagagcttc atctcggctg
     3061 cgataaattc gtcgctcatc actttggcgt agccgtctcc gctgccgccc gattcttgtg
     3121 ggaagtcgag tttcaggaac ttgccgccca tcgattcgat ttgttccgcc acttccaagc
     3181 gggtatcgaa cgcgcgtacc actgcgccga gcgagtttgc cgtaccgatc gccgccaaac
     3241 ctgccacacc tgcaccaatc accaaaacct gcgcgggcgg cactttgccg gcggcggtaa
     3301 tttgaccggt gaagaaacgg ccgaaggcgt tggcggcttc aattacggcg cggtagccgc
     3361 tgatgtttgc catcgaagac aaagcgtcca aagcctgcgc gcgcgaaatg cggggcacca
     3421 tatccatcgc cagcgcgttc actttcttgg cgcgcaaggc ttcgaccaaa gcctcgtttt
     3481 ggcgcggcca caggaagctg acgatggttt gaccttcgtt caaaagcggc agttcctgtt
     3541 cggacggcgc gttgacctta taaatcaaag ggcagaccca aaccgccgct ttgtcggcaa
     3601 cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa cctgcaccgc
     3661 tttcgacaac ggtttcaaag cccagtttgc ccagcagggc gacggtggcg ggcgtacagg
     3721 cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc tctgaatcct
     3781 ttttcgggtt gtttatatgt atcgtgggtt aaatttaaat cggggcgggg cggagcaacg
     3841 ccgtaccggt ttaaagccga ctcacttcaa atgttaatat attttagata atccccttat
     3901 aacgaatttt catcaggctg gcaatagttg cggcattttc ccgtgttgtc cgacacatac
     3961 tgccgtttgt gtttgaaatc gttttccgat tgcagtcccg cccgccgtaa cattctttgc
     4021 cgtttcacta tataatccgc attttttgag ccgccgttat gccgcacgcc ctcgtcctcc
     4081 aatttccctc cgccgcagcc ctgccttccg acttcccctt acgcctgccc gaacctgatt
     4141 gcgccgatga aaagcgtatg cgttttatcg ttgaagaagg gttttcttta agcgaaaaag
     4201 acgcggcgtt gcttggcagc cgtcaaatcg accacgccgt gttgccggat atggatttcg
     4261 acgaactcgg tttgattgtc agcgatatgg attcgacgct gattaccatc gaatgcgtcg
     4321 atgaaattgc ggcaggcgtg ggtttaaaaa acaaagtagc ggaaattacc gagcgttcga
     4381 tgcgcggcga actcgatttc gaacagtctt tacgcagccg cgtcgcgctg ttggcgggat
     4441 tggacgaacg ggttttggcg gacgtttatg aaaacgtttt gaagctctcg cccggtgcgg
     4501 aatttttgtt ggacgaatgc aaaaggcacg atgtgaaatt cctgctggtg tcgggcggct
     4561 tcacgttttt taccgaaagg ctgcaacaac gcctcggctt cgaataccaa cacgccaatg
     4621 ttttggaaat tgaaaacggc aggctgaccg gccgtctgaa aggcagaatc atcgacgcgc
     4681 aggcaaaggc agatttgttg cgcgaatacc gcagccgcct cggattgcag ccgcatcagg
     4741 tgttggcggt gggcgacggt gcgaacgata ttccgatgct caaagaagcg ggcataggcg
     4801 tggcttaccg tgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc aacttcggcg
     4861 gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg tcgtccgaaa
     4921 ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa ctttggtttg
     4981 ccctggcagc agcgggcgtt atcggcggtt tggtcggcat tgtgctgacg gaactgatgc
     5041 acttcataca gcatacggca tacggttatg gcgcggacgg cgtgtacact tcgttccgcg
     5101 aaggcgtggc acaggcttcc ggtatgcggc gcgttgccgt gctgacgctg tgcggcgcgg
     5161 tcgcaggcag cggctggtgg ttgctgaaac gtttcggcaa gccgcaaatc gaaatcaaag
     5221 ccgccttgaa acagccgttg caggggctgc cgtttctgac gacggttttc catgttctgc
     5281 tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcc ccgcgcgaaa
     5341 tgaccgccgc gtttgctttt gccggcggca aacgcttggg tttggatgaa ggcgaaatgc
     5401 ggctactgat tgcttgcgct tcgggtgcgg gtttggcggc cgtgtataac gtgccgctcg
     5461 cctccacact tttcattctc gaagccatgc tgggcgtgtg gacgcagcaa gccgtcgccg
     5521 ctgcattgtt aacttcagtc atcgccaccg ccgtcgcgcg catcggcttg ggcgacgtgc
     5581 agcaatatca tccggccaac cttaccgtca atacttcatt actttggttt tccgccgtca
     5641 tcggcccgat actgggcgta gccgccgtct ttttccagcg taccgcccaa aagttcccct
     5701 ttatcaagcg cgacaatatc aaaattattc ccttggccgt ctgtatgttt gcactcatcg
     5761 gcgtgatttc cgtttggttt cccgaaattt tgggcaatgg caaagcaggc aatcaactga
     5821 cctttggcgg attgaccgat tggcaacaca gccttgggct gaccgccgtc aaatggctgg
     5881 tcgtcttaat ggcgcttgcc gtcggcgcat acggcggtct gattaccccg tccatgatgc
     5941 tcggcagtac catcgccttt gctgctgcca ccgcgtggaa cagtgttttt cctgaaatgt
     6001 cctctgaaag cgcagccatt gtcggcgccg cagttttcct cggtgtttcc cttaaaatgc
     6061 ccctgaccgc catagccttt attttggagc tcacctacgc ccctgttgcc ttgctcatgc
     6121 cattatgtac aggcatggca ggtgcagtat gggtggcaaa gaaaatggga tttaaatagg
     6181 caaaagcaaa aggccgtctg aaaccaagtt tcagacggcc ttttacaata aaattgttaa
     6241 caatatttgc aaaaacctac tgccaaaaat gcgaaactgg gggataatac cgccctgaaa
     6301 attcatccca tactgattaa accttcaaca aaggaaatcc aaatgtcttc catcaaacgc
     6361 gccctgatca gcctatccga caagacaggc gcagtcgaat ttgcccaaac cctgcacaaa
     6421 ctcggtgtcg aaattctttc taccggcggt acagcaaaac tcttggctga tgcaggcgtt
     6481 cccgttatcg aagttgccga ctataccggt tttcccgaaa tgctcgacgg ccgcgtgaaa
     6541 accctgcatc cgaaaatcca cggcggtatt ctcggtcgtc gcgatttgga cgaacacgtc
     6601 gccaagatgg aagaacacgg catcggcaat atcgacctcg tgtgcgtcaa cctctacccc
     6661 ttcgctgcca ccatcgccaa accaaactgc acgctggaag acgcgattga aaacatcgac
     6721 atcggcggcc cgaccatggt gcgctctgcc gcgaaaaact ggaaacacgt cgccatcgtt
     6781 accgacaccg ccgatttccc ggccatagct gccgaactcg aagccaacaa cggcgcattg
     6841 agcgacaaaa cccgtttcaa cctctcgcgc aaagcattca gccataccgc ccaatacgac
     6901 ggtatgattt ccaattacct gacctcgctt tcagacgacg tcttgagcgg cacgcccgaa
     6961 atcgccggat tccccggccg gttcaatcaa agctggatta aagtgcaaga catgcgctac
     7021 ggcgaaaacc cgcatcagcg cgccgcgttc taccgcgata ttgaccccgc cgcaggcagc
     7081 ctcgctgcat acaaacaact gcaaggcaaa gaattgtctt acaacaacat cgccgatgcc
     7141 gatgccgcat gggaagccgt caaatccttc gacgtgcccg cctgcgtgat tgtgaaacac
     7201 gccaatccgt gcggcgtagc catcgcctcc aataccttgg atgcctacaa actcgcctac
     7261 gccaccgaca ccaccagcgc gttcggcggc atcatcgctt tcaaccgcga agttgacggc
     7321 gcaaccgtca aacaaattac cgacaaccag tttatggaag tcctcatggc gcctaagttc
     7381 accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg tgcgcgtatt ggaagtgccg
     7441 cttgaggcag gcgcaaaccg cttcgaactc aaacgcgtcg gcggcggact gttggtgcaa
     7501 acgcccgaca tccaccgcat cagccgcgcc gatttgaaag tcgtctccaa acgccaaccg
     7561 accgagcagg aatggaacga tttgctgttc gtctggaacg tcgccaaata cgtcaaatcc
     7621 aacgccatcg tattcggcaa aggcggtcaa acctacggca tcggcgcagg ccaaatgagc
     7681 cgcgtggaca gcacccgcat cgccgcccgc aaagcgcaag atgccggtct cgacctcaac
     7741 ggcgcgtgtg ccgcatccga tgccttcttc cccttccgcg acggcgtgga cgtgattgcc
     7801 gaacagggca tcaaagccat catccatccg gcaggctcga tgcgcgatca ggaagttttc
     7861 gacgcagccg acgaacacgg catcgccatg gtcgtaaccg gcatccgcca tttccgccat
     7921 tgatgcagat aaacaaggta atgccgtctg aagggctttc agacggtatt ttgcgctatt
     7981 ttgcgaaggt agggatgacg gttcgggtat tcctgacagg gtggattttc aaggtgttgt
     8041 atagggtgta ggaggattcg taaaaggtgg gatgcagggt gtgcttcagc ccgctgcatc
     8101 aaaaattttt ggagaaccgg cgggagtcgg cggttttggt ttcggcgggg acggtggaaa
     8161 tgggtaacat tgacggaatc gacggaagcg gtggactgaa gcccaccctt gtatattgga
     8221 atcaccgtat catagcaaca aaccgcccag ccgccacccg cgcccaccca aggcacacaa
     8281 ccgttgcgta gctcagggag cggcagggca acccatcgac acaaccggac agttgccgga
     8341 caacacaacc gaatgcaagg caggtttatg atgagtaccc aataccatta cgcaggtata
     8401 gtgaattaaa tctaaaccag tacagcgttg g
//
LOCUS       DAWISC010000081         8420 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWISC010000081 DAWISC010000000
VERSION     DAWISC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.081"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(457..732)
                     /locus_tag="WIW87_001272"
     CDS             complement(457..732)
                     /locus_tag="WIW87_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5174433.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(729..2012)
                     /locus_tag="WIW87_001273"
     CDS             complement(729..2012)
                     /locus_tag="WIW87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEZ5174434.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAVYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWLEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WIW87_001274"
     CDS             complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WIW87_001274"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ5174435.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3670..3855
                     /locus_tag="WIW87_001275"
     CDS             3670..3855
                     /locus_tag="WIW87_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174436.1"
                     /translation="MPSETGFASNFKPENIVSDGKIVNNADKPAHRIRIFPDRYRKRC
                     RLKAVSDGIVSICTPPL"
     gene            3886..4695
                     /gene="aroE"
                     /locus_tag="WIW87_001276"
     CDS             3886..4695
                     /gene="aroE"
                     /locus_tag="WIW87_001276"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ5174437.1"
                     /translation="MTALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            4698..5399
                     /gene="mtgA"
                     /locus_tag="WIW87_001277"
     CDS             4698..5399
                     /gene="mtgA"
                     /locus_tag="WIW87_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ5174438.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(5602..5677)
                     /locus_tag="WIW87_001278"
     tRNA            complement(5602..5677)
                     /locus_tag="WIW87_001278"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5642..5644),aa:Phe,seq:gaa)
     gene            complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WIW87_001279"
     CDS             complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WIW87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174439.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WIW87_001280"
     CDS             complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WIW87_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ5174440.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSADSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WIW87_001281"
     CDS             complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WIW87_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ5174441.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7765..8301)
                     /locus_tag="WIW87_001282"
     CDS             complement(7765..8301)
                     /locus_tag="WIW87_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5174442.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPGAHWFTRQHAAYITEHAGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1975 a         2286 c         2157 g         2002 t
ORIGIN      
        1 cttacaatat aaaacaaaca aatatagtgg attaacaaaa accagtacgg cgttgtctcg
       61 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
      121 cactataagt tgacatattg tgcgatgtcg agggctgtac cgtattttcc catcgtattt
      181 tcccgttctt tatatgagcg gcaggcatcc tcataaaaga agggataccg ataaggaaaa
      241 ataccccggc ataaaaaata tactgattaa tcaatacaaa tccaacaaac agttcatcgt
      301 attcttttct gttggcgtat aaaaaatact gatatatgcc ttcactttca tagggcgacg
      361 ctttatgtac cgtcctgtgt gttgaaacgt cggttttcct taccgcctgc cccgcttttc
      421 cctgattgct gccaactgcc gtccgaacct gaaccgtcag gtttcagacg gcattttttc
      481 tgcctgctcc cacaattgcc gtttttgtgc ggcggtcagt tttttgacgg cgatttcctg
      541 cctgagtgcc gttcctttat ccatcccgcc tgcaacgata cgcatcgcca ccggtttgaa
      601 tacgcgggta tatttcgcgc ctttaccggt tgtgtgggcg gcaagccgct gttgcggatt
      661 cgggctgatg ccgcaataga acgcgctgtt ttcacacaat atcaggtaaa cgctccagtt
      721 tgacgcgttc atctgcctgc atcctgagtt ttctccccgt tccaaggcgc aactttcagc
      781 agcagcagca tacccggtag ggcgagtgcg aaacacagtt ggaaaaacgg tacataaccg
      841 agccattcaa tcagataacc ggcaaaggaa ttgatgaccg tgcgcgggac ggcggacagg
      901 ctggtaaaca gcgcaagctg cgttgcggta aatgcgggat tggtttcacg cgccatatac
      961 gatacgaacg ccgccgtccc caaccccacg ccgaccgctt ccgcgccgat aactgccgcc
     1021 agcatcagcc tctcgcctgt gccgaccgtg tcgaaaggtc cgaaccctgc cagccataca
     1081 aaccccaaaa cggttacagc ctgcaccgcg ccgaatagcc acaaggcttt gtttacgccg
     1141 attttcagca tccacacacc gcccaagata cctgccgcca ctgccggcca cagtcctgca
     1201 tttttcgcaa tcaaaccgat gtcggtcttg ctgaaaccca tatccagata aaacggcgtt
     1261 gccaacgcgg ttgccatact gtcgccgagt ttgtaaagga agataaacag cagcacgcac
     1321 accgccgaag cgatgccctt gcgcgtaaaa aattctttaa acggctctac cacggtctgc
     1381 ttcaacgttt taggaacggc aggaggcaac acgggttcgc gcgcaagaaa cagcgtcatc
     1441 agaaggccgg gcagcataaa taatgaagtg ataacaaata cttctaacca cggcatcctg
     1501 tctgccaaca ccaaactcaa tgaaccggga atcagggcgg caatccggta ggcgttcaca
     1561 tgaaccgagt tgcccaaacc caattcttcg tctgacaaaa tctcgcgcct gaacgcatcc
     1621 aatacaatat cctgactggc ggaaaaaaaa gcgacaagca ccgacaagcc ggcaatcagc
     1681 ggcagatgat tacgggggtt taaaaaggca tagaccgcca aagccgccag caaccctgcc
     1741 tgcgtcagca gcatccaccc gcgccgccgt cccaaaacgg gcagcctgac cgcgtccatc
     1801 agcggcgacc acaaaaattt ccaagtaaac ggcagaccga ttaacgccat cagcccgatg
     1861 ctcttcaaat ccacctgctc gctgcgcaac cacgccggaa tcaggttaat cagaaagtac
     1921 agcggcagcc ccgaggtaaa ccccgtaaaa atacagacaa gcatattgcg ggaaaagatt
     1981 tgcccgataa aacctgattt cgatgcagtc atcgttcgtg cagcccaaaa aaaacaaaag
     2041 cctattgtag caaatatcgc ccgcctgctg aaaaatgccg ccccgcgccg ccggtttgcc
     2101 gttcaaatgc aaaatgccgt ctgaaactgt gttcagacgg cattgctttt ctgaaaacca
     2161 gacgtgctta caggctgtaa tacatttcaa attccagcgg gtgcggcgcc atgcggatgc
     2221 ggcgtacgtc ttcttctttg aaagcgatgt agctgtcgat ccagtctttg ctgaacacgc
     2281 cgccgcgcag gaggaattcg tggtcggctt tgagggcggc cagtgcttct tccaaagaag
     2341 cgcaaacggt cggcaccaat gcatcttctt ccggcggcag atcgtacagg tttttatcgg
     2401 cagggtcgcc cggatggatt ttgttttgaa tgccgtccaa acccgccatc aacagggcgg
     2461 caaatgccaa atacgggttg gcggtcggat cggggaaacg cgcttcgatg cggcgcgcct
     2521 tgctgctgtt cacggacgga atgcggatgg aagcggaacg gtttttggcg gagtatgcca
     2581 gtttggtcgg cgcttcaaag tgcggcacga ggcgtttgta ggagttggtg gacggattgg
     2641 taatcgcgtt caaggctttg gcgtgtttga tgatgccgcc gatgtagtag agcgcggtgt
     2701 cgctcaagcc ggcatagccg tcgcctgcga acaggttttg accgtctttc caaatggatt
     2761 ggtgaacgtg cataccgctg ccgttgtcgc ccataatggg tttgggcatg aaagtggcgg
     2821 ttttgccgaa gttgtgggca acgttttgaa tcacatattt catgtcttgg gtttggtcgg
     2881 cgcgtttgac caaagtagca aagcgcgtgc cgatttccat ttggctgccg gtgccgactt
     2941 cgctgtggtg cacttccact tcaataccga gttcttccaa aatgtttacc atcgccgaac
     3001 gcaaatcctg accgcagtca atcggtgcaa caggtgcgta accgcctttg acggtcgggc
     3061 ggtggccggt gttttgaccg tccatatgca gaccgcttgc ccacgcgccg ctttcggacg
     3121 tgatttcgta acgggttttg tgcatatcgg tttcaaattc tatgccgtcg aatacgaaaa
     3181 actcgggttc gggaccgaaa taggcggtct cgccgatgcc ggaagatttc aaataggctt
     3241 cggctcggcg ggcgatggag cgcgggtcgc ggtcgtaacc ctgaccgtcg gcgggatcga
     3301 taacgtcgca agtcaacaca acagtcgcat catcataaaa aggatcgacg aaggctgtag
     3361 acgcatcggg gcgcaactgc atatcggaag cctgaatgcc tttccagccg ccgatagacg
     3421 aaccgtcaaa cgcctgaccg ttttcgaacc actcttcggg gtcttccaac acgatgcgcg
     3481 caggcacggt aaagtggtgc tgcttgcctt tggtatcggt aaagcgcaaa tcgacaaagc
     3541 gggcttcgct ttcttcaatc aattttacgg cgtttttaat agacattttt cagctcctgg
     3601 aaaagggaaa tagcaaaata tgcggaacgc ggcggcttga cgcaaagtcg gcacgatttt
     3661 accacgccga tgccgtctga aaccggtttt gcgtcaaatt tcaagccgga aaatatagtt
     3721 tcagacggca agattgtcaa caatgcggac aagccggcgc accgcatccg gatttttccc
     3781 gaccgataca ggaaacgatg ccgtctgaag gcggtttcag acggcatcgt ttccatctgt
     3841 acgccgccgt tataaaatag ccttcccgtc ttttggaaca cgaccatgac cgccctcccc
     3901 cgctacgccg ttttcggcaa ccccgtcgcc cacagcaaat cgccgcaaat tcatcaacaa
     3961 tttgcccttc aggaaggcgt tgacattgaa tacgaacgca tttgcgccga catcggcggt
     4021 ttcgcgcagg cggtttcgac attttttgaa acaggcggtt gcggggcaaa cgttaccgta
     4081 ccgttcaaac aggaagcgtt tcatctggcg gacgagcatt ccgaacgcgc attggcggca
     4141 ggtgcggtca atacgctgat tccgttgaaa aacggcaagc tgcgtggcga caacaccgac
     4201 ggtatcggtt tgaccaacga catcacgcag gtcaaaaata ttgccatcga gggcaaaacc
     4261 attttgcttt tgggcgcagg cggcgcggtg cgcggcgtga ttcctgtttt gaaagaacac
     4321 cgtcctgccc gtatcgtcat tgccaaccgt acccgcgcca aagccgagga attggcgcag
     4381 cttttcggca ttgaagccgt cccgatggcg gacgtgaacg gcggttttga tatcatcatc
     4441 aacggcacgt cgggcggtct aaacggtcag attcccgata ttccgcccga tatttttcaa
     4501 aactgcgcgc ttgcctacga tatggtgtac ggctgcgcgg caaaaccgtt tttagatttt
     4561 gcacgacaat cgggtgcgaa aaaaactgcc gacggactgg gtatgctagt cggtcaagcg
     4621 gcggcttcct acgccctctg gcgcggattt acgcccgata tccgccccgt tatcgaatac
     4681 atgaaagccc tataaacatg ttccgcatca tcaaatggct gattgccctg cccgtcggca
     4741 tctttatctt tttcaatgcc tatgtgtacg gcaacatcat tacctaccgc gccgtcgcgc
     4801 cccatcggac tgcctttatg tcgatgcgga tgaagcagtt tgaacaggaa ggtcgcgatg
     4861 tcgcactgga ttaccgctgg atgccctaca aacgcatttc caccaacctg aaaaaagccc
     4921 tgattgcttc cgaagatgcc cgtttcgccg ggcacggcgg cttcgattgg ggcggcattc
     4981 aaaacgccat caggcgcaac cggaacagcg gcaaagtgaa ggcgggcggc tcgaccatca
     5041 gccagcagct tgccaaaaac ctgtttttaa acgaaagccg cagctatatc cgcaaaggcg
     5101 aagaagcggc gattaccgcg atgatggaag ccgttaccga caaagacagg atttttgaac
     5161 tgtatttaaa ctcaatcgaa tggcactacg gcgttttcgg cgcggaagcc gcgtcccggt
     5221 atttttatca aatacccgcc gccaagctga ccaaacagca ggcggcaaaa ctgacggcgc
     5281 gcgtccccgc cccgctctac tacgccgacc atccgaaaag caaacggctc cgcaacaaaa
     5341 ccaatatcgt gctcaaacgc atgggttcgg cagagttgcc tgaaagcgac acggactgag
     5401 tgccgccgca atataaaatg ccgtctgaaa tatcattcag acggcatatg ctttctgcct
     5461 ttaccctctt ctccaaaacg caacggggcg gtcattatca tttttgccct tctacataac
     5521 ggtcagacac aaaacgtgtc cgacacggac aacttcccaa aaacggcagg caaaaaaaat
     5581 aaccgcttca aggcggtttt ttggtggcca gaggcggaat cgaaccgccg acacacggat
     5641 tttcaatccg ttgctctacc aactgagcta tctggccttt gtctgtctcg ttaagagatg
     5701 cgtattaaac caaattacgg gacgacatgc aagcatatcg ggcaatattc tttgaaaaca
     5761 ggtttaagtc aatgttttaa atttgaatta atttatgtaa aaacaacata aatccgtatt
     5821 tttgccgcct gcccgacgat attaggtcgc ccgtctgaaa aatattttca atatttgaag
     5881 ttcttaccca gataaacaga acgaacctgt tcgtttccga ccaaatcatc aggttttccc
     5941 gatgccaaca ccgtgccgtc tgaaataatg taggcccgat cgcagatgct gagggtttcg
     6001 cgtacgttgt ggtcggtaat cagtacgccg ataccgcgcg atttgaggaa accgatgatt
     6061 ttctggatgt cgatgacggc aatcggatcg acgccggcaa aaggttcgtc caacaaaata
     6121 aaatgcggtt tcatggcgag tacgcgggcg atttcgacgc gccgccgttc gccgccggac
     6181 agcgacggcg cggggctgcg gcgtaagtgt ccgatattga ggtcggcgag cagtttttcg
     6241 atttccctgt cgatttgatt tttatctttg gttctgattt ccaagatggc gcggatgttt
     6301 tgttcgacgg tcattttgcg gaatatcgag gcttcctgcg gcaggtagcc gacaccgagg
     6361 cgggcgcgtt cgtgtatggg caggtggcgc aattcttgtc cgtctagggt tacgctgcct
     6421 gcgtcggcgg cgatgagtcc gacaatcatg tagaagctgg tggttttacc cgcaccgttg
     6481 ggcccgagca gtccgatgac ttcgccgctt tcgatttcga gggagaagct tttaacgact
     6541 tggcgttttt tgaaactttt ttgcaggttt tgaacaacaa ggcggctgac gtttgcactc
     6601 ataaatatct cttcaatatc ttcggtcggc atatgccgtc cgaaccgggt ttatatttca
     6661 gacggcattt tgggattatt cggatttttg cgtactcgaa ggctggataa cgacgctgac
     6721 cctgccggat ttggaagcgg attttgcgcc ggattttgtg ctgccgctga tggtatagac
     6781 ttcggttttg gtgttgtatg taatcaccgc accttcggcg acatcgccgc cgcgctgtac
     6841 tttggcatta ccggttaaga ctacggtgct gtctgcagat gaataagcaa cgttgtttgc
     6901 ctgtccgcgc accgtgcctt tgccgccgtc caatgtctgg ctgaagcgga ctggcgaacc
     6961 ttccgccctc acggattcgc cgcctttgcc gccgcgtgta acattgacgc gggcggcgga
     7021 aatattgagc gtaccctgtc tgatgacgac gtttccgctg aatgtggtgc tttggttggc
     7081 ttgatcgagc gaaccttggt cggcctcaat ctgaataggc tgcctgctgt cgctttgaag
     7141 ggcaaaagcg ggggacgccg aaaaaaatgc tattaaaaca aatagcttac atatcttttg
     7201 tatcataaat cgtggctttc actttagatg agaagttcaa catgcctgtt ttgtggtcgt
     7261 aagtcatgcc gcccgcctga ccgtgcgatg caccatattg gaaactgaca ggcgtatcgg
     7321 tttgggcata ttgagattcg gtatcgacgt gcagcttttc ggcttcaact ttacccgcct
     7381 gccgtttgcc gtcggcggtt ttggtcagca caacgttgtt tttaaaaaga acctgtttgt
     7441 tttcggtatg gtaaacggct tcgtcgctgc cgacttcgta caacaacctg ccttcttgga
     7501 agaacacgag atgcggcgaa tcaaaatgga tgtcgctgct ttccggaaac tgtttcgcgc
     7561 ccttcgcgct caaatgttct ttcaagtatc cctgttcgtc aaaccgcctg ccgtccaagc
     7621 cgtccattgt gtattgcggt tcgtcgggat tgagcctgac ttcttcaatc tcgacttcgc
     7681 tgatacgacc caaccatgcc gacaggctgc ccaaggcaac cgccaatatc aatgggaacg
     7741 caattccgta ccgccatctt actttcattt gatgtactcg ttcaaagccg cgcccaaagt
     7801 cccttgcgcc tgcataatca ggtcgcacac ttcgcgcacc gcgcctgcgc cgcccgcgtg
     7861 ttccgtgata taggcggcgt gttgccgcgt aaaccaatgc gcgccgggga cggcaaccgg
     7921 caatccgcag cgcaccatta ccggcaaatc gaccacgtcg tcgccgacaa aggcgcactc
     7981 ggcttcttcc acgcccgcct gcgcgcgcaa ttcttcatag gcggcacgtt tgtcgctgat
     8041 acctttgaaa tagtaattta tgcccaactg tttgacgcgg atgccgacgg agggcgcgtc
     8101 ccggcccgtg ataatcgcag tctgcacgcc gcttgcctga agcattttca gaccgtgtcc
     8161 gtccagtgtg tgaaacgatt tgatttcttc gccgttatcg cggataaaga tgcgcccgtc
     8221 ggtcaaaacg ccgtccacat ccaggatcaa cagtttgatt ttggcggcgc gcgcctgtaa
     8281 ttcgggagaa attgcctgca tgggatttcc ttgttttcag acggcatatc ttaccacaaa
     8341 tgccgtctga aacggctgca acgccttata tagtggatta acaaaaatca ggacaaggcg
     8401 acgaagccgc agacagtaca
//
LOCUS       DAWISC010000082         8359 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWISC010000082 DAWISC010000000
VERSION     DAWISC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.082"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..71
                     /locus_tag="WIW87_001283"
     CDS             <1..71
                     /locus_tag="WIW87_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5174443.1"
                     /translation="QGEPNLSPKDLAGKVLSEAVTF"
     gene            96..2210
                     /locus_tag="WIW87_001284"
     CDS             96..2210
                     /locus_tag="WIW87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ5174444.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HICKDGKEAEAVVGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARSN
                     CSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2347..3606
                     /locus_tag="WIW87_001285"
                     /pseudo
     CDS             2347..3606
                     /locus_tag="WIW87_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
     gene            3973..5379
                     /locus_tag="WIW87_001286"
     CDS             3973..5379
                     /locus_tag="WIW87_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ5174445.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5597..6811
                     /gene="gltS"
                     /locus_tag="WIW87_001287"
     CDS             5597..6811
                     /gene="gltS"
                     /locus_tag="WIW87_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ5174446.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6910..7926
                     /locus_tag="WIW87_001288"
     CDS             6910..7926
                     /locus_tag="WIW87_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ5174447.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7937..8134
                     /locus_tag="WIW87_001289"
     CDS             7937..8134
                     /locus_tag="WIW87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174448.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8152..8301
                     /locus_tag="WIW87_001290"
     CDS             8152..8301
                     /locus_tag="WIW87_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174449.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2147 a         2429 c         1934 g         1849 t
ORIGIN      
        1 tgcaaggcga gccgaacctg tcgcctaaag acttggcagg caaagtattg tctgaagcgg
       61 taacgtttta aaaataattc aggccgtctg aaagaatgtt cctcttttca gacggcctac
      121 aatccattaa taacaataat cgacgaaaac gcattgtgaa aaacgcctac atcccctctc
      181 gcggcatccg caaaatcccc catctctcca ccctattgcc tgaatttcat atctgcaaag
      241 acgggaaaga agcagaggct gttgtcggct ggggtttgcg cccgacgaca cacaaagcgc
      301 gtgcttttgc cgctgaacac cagcttccct ttattgcttt ggaagacggc tttttacgat
      361 cgctcggact gggtgtcgcc ggttatccgc cctactctat cgtctatgac gacatcggca
      421 tctactacga caccacacgt ccttcgcgtt tggaacaact gattcttgcc gccgatacca
      481 tgccgtctga aaccttggct caggcgcagc aggcgatgga tttcatcctg caacaccacc
      541 tgtccaaata caaccacgcg cccgaacttt cagacgacca tcctttacgt tccccatcca
      601 aacccgaaac cgtcctcatc atcgaccaaa ccttcggcga tatggccatc caatatggcg
      661 gcgcagacgc ctctacgttt gaactgatgt ttcagacggc cttaaatgaa aacccgcaag
      721 ccgatatctg ggtaaaaacc catcccgatg ttttgtgcgg caaaaaacaa ggctatctga
      781 cccaactggc gcagcaacac cgcgtccatc ttttggcaga agacatcaat ccgatttctt
      841 tgttgcaaaa cgttgataaa gtttattgcg ttacctcgca aatgggtttt gaggcgcttt
      901 tgtgcggcaa accgctgacc actttcggcc tgccgtggta tgccggatgg ggtgtaagcg
      961 acgaccgcca tcctgaaatc aaccgccttg ttcaaaccca acgccgcgcc acccgcaact
     1021 tgctgcagct cttcgccgca gcctatctgc aatacagccg ctacctcaac cccaataccg
     1081 gcgaagcagg cagcctcttt gatgtcatcg actatctggc gacggtcaaa cgtaaaaacg
     1141 acaaattgcg tggcgagtta tattgcgtcg gtatgtcttt gtggaaacgc gcggttgcca
     1201 aaccgttctt taacgtaccc tcttgccgtc tgaaatttat ctcttccacc caaaaactgg
     1261 caaggtcaaa ctgttccgac gatgcacgca tcctggcttg gggcaacggc aaagaggcca
     1321 tcgtccgctt tgccgaacaa caccacatcc ccctgctgcg catggaagac ggctttatcc
     1381 gctcggtcgg actcggctcc aacttagtgc cgccgctgtc gctcgttacc gacgatatga
     1441 gcatttattt caatgccgaa accccgtccc gtcttgaata catcctacaa aaccaaaact
     1501 tcgacgatca agactttcag acggccttga agctgcaaaa aatgctgacc gaaaaccaca
     1561 tcagtaaata caacgtcggc agctcagact tcaccgcccc gtcaaccgac aaaaccgtga
     1621 tcctcgttcc cggccaggtt gaagatgatg cgtctatccg ctacggttcg ccccaaatct
     1681 accgcaatct ggatttgctc cgtaccgtac gcgaacgaaa ccccaatgcc tatatcatct
     1741 acaaaccgca tcccgatgta gtcagcggta accgcatcgg ccatatttcc cctgaagatg
     1801 ctgcacgata tgccgaccaa accgccgaac aagccgacat cctgacctgt ctccaatacg
     1861 cagacgaaat acataccatg acttcgctga ccggttttga agccttgttg cgcggcaaaa
     1921 aagtcagctg ctacggcctg cctttttacg caggctgggg gcttacccaa gatctgctcc
     1981 ccatcccgcg ccgtagccgc agacttgagc tttggcagct gattgccggc acgctcatcc
     2041 actatcccga ctacatccac cccgaaaccc atcaggccat aaatgcagaa accgcagccc
     2101 aaatcctgat acgacaaaaa aatatgcaaa aaaacaacaa cggattacat cgcgggtgct
     2161 ttgccaaaaa attaggtaaa atcaaacaac tatatcgatc tttcaaataa ataccatcaa
     2221 agttaacgat gcgtcataaa cttgcctcta ttgcggcatc attgcctttg catcgttaat
     2281 tctcttggcg tatgcttgaa agttcaacct aaaactatta cataaaaaac aaaaccacat
     2341 tgcaacatga aacagaccgt cctcaaaaat aacctgcaaa acctgcttga aagcgcagaa
     2401 aatatcctgc tgcttcaagg ccctgtcggc gatttttttc tgcgccttgc cgactggctg
     2461 actgcaaacg gcaaaaccgt acataaattc aactttaatg caggcgacga ctatttttat
     2521 ccgcccactc aagcgcatac cgttgttttt aacgacaact acgatgcctt tcctgagttt
     2581 ttgcaagaat acatcactca acatcacatc taggccgttg tctgctttgg cgacacacgc
     2641 ccttatcacg tcattgcaaa acgcattgca aacgaaaacc aagccagttt ctgggcgttt
     2701 gaagaaggct atttccgccc ctactacatc accttagaaa aagacggcgt caacgcattt
     2761 tccccgttgc cgcgccgtgc cgactttttt cttgaacaat tccctaagct tgcccagcaa
     2821 gaatataaag cgccaacgcc ggtacacggc ggttttacgc ccatggcaaa aaacgctatc
     2881 cgttactata tcgagttgtt ccgcaatcca cgcaaatacc ccgactacat ccaccaccgc
     2941 gcacccaatg ccggccatta cctcaaaccg tggtcgctct ccatcctcaa gcgtttgaac
     3001 tactatattg aagacatcca aatcgccaaa cgtgtggaag caggcaaata cggcaagttt
     3061 tttattgttc ccttacaggt attcaacgac agccaagtcc gtatccattg cgactttccc
     3121 agcgtccgca gcttcctgct ccatgttttg agttcatttg ccgagcacgc gcctgccgat
     3181 accaacatca tcatcaagca tcatccgatg gaccgcggtt ttatcgacta ctggcgcgac
     3241 attaaacgct ttatcaaaga acaccccgaa ctcaaaggcc gtgtgattta tgtccatgat
     3301 gtccccctgc ccgttttcct gcgccacggt ctcggcatgg tcaccatcaa cagcaccagc
     3361 ggcctgtccg gactgattca caatatgcca gttaaggttc tcggccgtgc ctattatgat
     3421 attcccggca ttactgacca aaataccttg gcagaatttt ggaatcatcc gacaccgcct
     3481 gacaaagagc tgttccatgc ctaccgaatg taccacctca acgtgaccca aattaacggc
     3541 aacttctaca gtcaggtgtt tttccccaac aaaaaaacct ccaactcttc cacaccagta
     3601 atctgactta gcgaaggaag ttcaggccgt ctgaaaacat ttcagacggc ctgaaacaat
     3661 caatacctta gctactgcca tgtaaataaa acacaaaaat ctgcatttat cattaacaat
     3721 aaattacaaa aacagtataa tgaccgagct gccatgagcg cataccgact caacctgagc
     3781 cctttgtaac acacaaaata tggatatatc cctaggcaaa acaatataac aagccaaaca
     3841 tcctaaagat aagccggcaa ggcaatacac tctataaaac tatgccgagc aaaattttta
     3901 caaagccctc aaccggtatc gccgcccata tgccgcagca tccgtcttcc actttatatc
     3961 cgcccgcaaa ccatgaccgc cgctcctgat atcctctacc ggcaagccgc cgcccttttg
     4021 gaacaatcca ataccgccca agccctgccc ctgttgcaac aggcggcaga gcaaggttat
     4081 gcggaagctg ctttcgtatt gggcaaccat ctgctgcaaa acggccaacc ggagcaggca
     4141 ctttcatggt tggaagccgc cgcggcccaa cgccatccca aagcactctt ctccctgctg
     4201 caacaacgcg aacacaacgg caccccgacc ggacagcttc tcaacgacta tgcctggctg
     4261 ggtgagcagg ggcactcaga agcccaatta atcctcatgc gttaccacgc gcaacgcaac
     4321 gatccacaat cgctctactg ggcggaactt gctgccgccc gatatgccgc acctgcgtat
     4381 taccatctgg cacgccatca tcaacgccaa ggcgacgttg aaacagccat cgaacaatac
     4441 gaaaaagcgg cagcactcgg cgtaactgcc gcctgctggc aacttggtca aatctacttc
     4501 tacggtacag gtgtcagccc caaccacgca caagccgaac actatctcga accagccgca
     4561 caagccggcc acatcgccgc acaaacgctg ctggctgacc ttcttgccgc ccaacgcaaa
     4621 cctgaagcct tggaatggta tcgtcgtgcc gccgataagg aacaagcgga agcacagtct
     4681 aagctggccc aatacgccct gaccggcgaa ctttccgaac gcgatccgtt ccaagcggca
     4741 cgatatgcca aagccgctgc cgagaaaaac catcctgaag ccctgaaaat catgggcgac
     4801 ctctaccgct acggtctcgg tatcaaagcc gacaaccata tcgcgcaaga ttactaccac
     4861 cgtgccgccg cgctgggttc tgccgccgca gcacaaaaac tcatcagcga cgccgcgctg
     4921 taccatccgc aacaatacga acaaatcaaa actgccgccc tgcaacaaca acaaaccgaa
     4981 accatctacc gtttggcgga agcacaagcc tgcgccatcg gccgtcccgc cgactacaat
     5041 gccgcgcgaa aaaattacat ggaagctgcc gggttccacc ataaaaacgc agcggcagcc
     5101 ttaggccgca tctaccatta cggcctcggt acggcgcaag atcctcgggc ggctgcacac
     5161 tggtacgcca ttgctgccga acaaaaccac ccttccgccc aataccacct cgcctgtttt
     5221 tactatcacg ggcaaggtgt cggctgtcat gttccgaccg cctgctactg gctgcaggcc
     5281 gccatcggca acggccacac ttcggccgaa tcattaatat ccctattaga acaatggcga
     5341 cgcgaagcac accatgccat cggacaaaag gccgtctgaa aagatttaca ctcgcatttt
     5401 ttgacaatct ttaactattc ccctaatatt tgccagttat ttttcacgga cacgccattg
     5461 ttttcatttc tttctgaaaa caccttgtcc gcgcatcaat accatgacac tcggcggata
     5521 acgccaagcg ttgaaacaca ctacatccgg aacaaaaacg gatgctcgga aaaatatttc
     5581 taggaggtga aacaacatgg aatgggaatt caacagttat tacacactga ttgccgccac
     5641 gctcgtgttg ctggttggta aatttctggt tcaaaaaatc aaattcttac gagacttcaa
     5701 tattcccgag ccggtagccg gcggtttgat tgccgctatc gtcctgttcg ccctgcacga
     5761 ggcgtacggc gtgagcttca aatttgagaa accgctgcaa aatgcgttta tgctgatttt
     5821 tttcacgtcc atcggcttga gcgcggattt ttcccgtttg aaggcgggcg gtttgccgct
     5881 ggtggttttt accgcgattg tgggcggatt tatcttggtg caaaactttg tcggggtcgg
     5941 actggctacg gctttgggtt tggatccgct catcggtctg attaccggtt cggtgtcgct
     6001 gacgggcgga cacggtacgt caggtgcgtg gggacctaat tttgaaacgc aatacggctt
     6061 ggtcggcgca accggtttgg gtattgcatc ggctactttc gggctggtgt tcggcggcct
     6121 gatcggcggg ccggttgcgc gccgcctgat caacaaaatg ggccgcaaac cggttgaaaa
     6181 caaaaaacag gatcaggacg acaacgcgga cgacgtgttc gagcaggcaa aacgcacccg
     6241 cctgattacg gcggaatctg ccgttgaaac gcttgccatg tttgccgcgt gtttggcgtt
     6301 tgccgagatt atggacggct tcgacaaaga atatctgttc gacctgccca aattcgtgtg
     6361 gtgtctgttt ggcggcgtgg tcatccgcaa catcctcact gccgcattca aggtcaatat
     6421 gttcgaccgc gccatcgatg tgttcggcaa tgcttcgctt tcgcttttct tggcaatggc
     6481 gttgctgaat ttgaaactgt gggagctgac cggtttggcg gggcctgtaa ccgtgattct
     6541 tgccgtacaa accgtggtga tggttttgta cgcgactttt gttacctatg tctttatggg
     6601 gcgcgactat gatgcggcag tattggctgc cggccattgc ggtttcggct tgggtgcaac
     6661 gccgacggcg gtggcaaata tgcagtccgt cacgcatact ttcggcgcgt cgcataaggc
     6721 gtttttgatt gtgcctatgg tcggcgcgtt cttcgtcgat ttgattaatg ccgcgattct
     6781 caccggtttt gtgaatttct ttaaaggctg attttccgcc tttccgacaa agcacctgca
     6841 aggtttaccg cctgcaggtg cttttgctat gatagccgct atcggtctgc accgtttgga
     6901 aggaacatca tgtatcggaa actcattgcg ctgccgtttg ccctgctgct tgccgcttgc
     6961 ggcagggaag aaccgcccaa ggcattggaa tgcgccaacc ccgccgtgtt gcaaggcata
     7021 cgcggcaata ttcaggaaac gctcacgcag gaagcgcgtt ctttcgcgcg cgaagacggc
     7081 aggcagtttg tcgatgccga caaaattatc gccgccgcct acggtttggc gttttctttg
     7141 gaacacgctt cggaaacgca ggaaggcggg cgcacgttct gtatcgccga tttgaacatt
     7201 accgtgccgt ctgaaacgct tgccgatgcc aaggcaaaca gccccctgtt gtacggggaa
     7261 actgctttgt cggatattgt gcggcagaag acgggcggca atgtcgagtt taaagacggc
     7321 gtattgacgg cagccgtccg cttcctgccc gtcaaagacg gtcagacggc atttgtcgac
     7381 aacacggtcg gtatggcggc gcaaacgctg tctgccgcgc tgctgcctta cggcgtgaag
     7441 agcatcgtga tgatagacgg caaggcggtg aaaaaagaag acgcggtcag gattttgagc
     7501 ggaaaagccc gtgaagaaga accgtccaaa cccacgcccg aagacatttt ggaacacaat
     7561 gccgccggcg gcgatgcggg cgtaccccaa gccgcagaag gcgcgcccga accggaaatc
     7621 ctgcatcctg acgacggcga gcgtgccgat accgttaccg tatcacgggg cgaagtggaa
     7681 gaggcgcgcg tacaaaacca gcgtgcggaa tccgaaatta ccaaactttg gggaggactc
     7741 gataccgacg tgcaaaaaga gttggtcggc gaacaacgca agtgggcgca ggaaaaaatc
     7801 agcaactgcc gacaagccgc cgcgcaggca gaccggcagg aatacgccga atacctcaag
     7861 ctgcaatgcg acacgcggat gacgcgcgaa cggatacagt atcttcgcgg ctattccatc
     7921 gattaggggc aaaccgatga ataccgtccc aaaaagcagg attcccgtca aaccgctgcc
     7981 cgaaaaaacc acagacgaag ccaaagtcga aaaatggcgg cagctcggtg cggaacacgg
     8041 tttgtcgggc gaatgggcag ttgccgtcag attgggcgaa aacggtttta ccgaagaaca
     8101 gatggaaaat atcgccaacc tgttcggcag ataaagagaa aattgacgga aatgccgtct
     8161 gaaaccctgt tatcggtttc agacggcatt ttgaccaata cggtacgcag gcgcaaaaca
     8221 gccggctttt cctgtgttgc ctatgctgat gtttcaacac acaggacgat acaaaaaacg
     8281 tcgccctatg tgccgtcctg attcggaagg gttacgctcc ttccaaatat agtggattaa
     8341 caaaaaccgg tacggcgtt
//
LOCUS       DAWISC010000083         8268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWISC010000083 DAWISC010000000
VERSION     DAWISC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.083"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..1505)
                     /gene="zwf"
                     /locus_tag="WIW87_001291"
     CDS             complement(60..1505)
                     /gene="zwf"
                     /locus_tag="WIW87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ5174450.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2072..3907
                     /gene="edd"
                     /locus_tag="WIW87_001292"
     CDS             2072..3907
                     /gene="edd"
                     /locus_tag="WIW87_001292"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ5174451.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            4088..4726
                     /locus_tag="WIW87_001293"
     CDS             4088..4726
                     /locus_tag="WIW87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ5174452.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            4741..5037
                     /locus_tag="WIW87_001294"
     CDS             4741..5037
                     /locus_tag="WIW87_001294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174453.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     VQFIRQQPDSPYYAAEKMPSETHSDGIHLKKTTDSTPINPLPKPLSSLGFRRRV"
     gene            complement(4985..6026)
                     /locus_tag="WIW87_001295"
                     /pseudo
     CDS             complement(4985..6026)
                     /locus_tag="WIW87_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            6189..7229
                     /gene="mutY"
                     /locus_tag="WIW87_001296"
     CDS             6189..7229
                     /gene="mutY"
                     /locus_tag="WIW87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ5174454.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQILAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENALWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            7646..8206
                     /locus_tag="WIW87_001297"
     CDS             7646..8206
                     /locus_tag="WIW87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ5174455.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         2102 a         2279 c         1956 g         1931 t
ORIGIN      
        1 tgctttactt acgttcagac ggcatttcaa accccatgcc gtctgaacgc attattgtat
       61 tactgctctt cgtgccactt gtgtccgtcg cgcgccaata gttcgcgcgc ggcttcaggc
      121 ccccacgagt gtgcgccata gccgtgcggc ggcgtggtgt tatttgtcca gttttccaaa
      181 atcggcatca catattccca cgcggcttca agttcgtcgc ggcggttaaa caaagcgagt
      241 ttgccgttaa tcacatccag cagcaggcgc tcgtaagctt ccgcgcggcg gctttccaat
      301 gctttgccca aatcggttgc cagcggcacg gtttcgacct tatttcctgc ccccggggtt
      361 ttcatctgcg tatagaggcg cacggattca tatggttgca actcgataac gagccggttg
      421 ggcgcggtgc gactgccttc aaaaatatgg ctgttcaaat ctttgaagtt caaaacgatt
      481 tccgccactt tgcccgccat gcgcttgccg gtacgcaggt agaagggaac gcccttccag
      541 cgttcgtttt cgatttcggc tttaatggcg acgtaggttt cggtaaagct gtcttgcgga
      601 acgttgattt cttcaagata gccgttcatg cctttggcgg cggtatattg tccgcgcacg
      661 acattttcat tgacagattc gacggtcagc ggcttcaatg acttgatgac tttgactttt
      721 tcatcgcgca ccgcgtcggc atccaagctg gcgggggctt ccatcgcagt catgcacagc
      781 atttgcatca agtggttttg caccatatcg cgcaacgcgc cggtaatgtc gtaaaactcg
      841 ccgcgttctt ccacgcccaa ctgctcggcg atggtcagct gcacgctttc gatgtattta
      901 ttgttccaaa gcggctcgaa catcacattg gcaaaacgca aggccagcag gttttgcagg
      961 ctttctttac ctaaataatg gtcgatacgg tagatttgcc cttctttgaa ataacgcgca
     1021 acatcggtat tgatttgttg ggaagatgcc aaatccgtac ccaacggttt ttccaaaact
     1081 acgcgcacat tgtcggcatt caaaccgatc gcagcaaggt tttcacaggc ttgcgcgaag
     1141 aatttcggcg cggtggaaag atagatgacg acattgtcgg tttctttgcg cgctttgacc
     1201 aaatcgccca aagcggcaaa atcgtccggc tgcgtaacat cgactttgag atatgcgaaa
     1261 cgttcgacaa acgatgccca agcctcatcg gaaaaatttt ctttcacatg gattttggaa
     1321 ctggtttcca ccttcgccag aaaaccttcg gtatccaact cgctgcggct gacccccaaa
     1381 atacgccctt cgggatgaag cagaccggca acatgcgcct ggtacagaca gggcaacagc
     1441 ttgcgcatcg ccaaatcgcc ggtcgcaccg aacaacacca aatcaaaatt tgtttgtgta
     1501 ctcatcgtat tatctcgtca ggaaagaatt tttcgatgcc gtctgaaacc tgtttccccc
     1561 atcacgctgc atcgcaatat cggaaacaaa ggcaggcggc ataatgagta gtaatactac
     1621 acaccgctac actttttgtc tattcccatt tttacaattt atttgaccta gtccaaaaat
     1681 cgggcaggtt tcccctattc cgttacaaca atcgaaagat tctgcgattt aaatcaaatt
     1741 tcttttcaat gcctgatttt tttgtaacaa aattacaaat tttgtactat aataacaccc
     1801 gcttcccact ttcagacggc atacctttta aaatatagtg gattaacaaa aatcaggaca
     1861 aggcgacgaa gccgcagaca gtacagataa tacggaaccg attcacttgg tgcttcagca
     1921 ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtactgtt ttttgttaat
     1981 ccactatact taccgtctga atacccgata caaaaatcag aaacgcacaa acaaatcccc
     2041 aatacccccc cgttccgaca ggagaccgac cgtgaacact actcctatcc actccaaact
     2101 cgccgaaatc accgggcgca ttattgaacg cagccgtccg acgcgtgaaa aatatctggc
     2161 gaagatccgc agtgccaaac aaatgggacg cttagagcgc aaccagctcg gctgcagcaa
     2221 cttggcacac ggctatgctg ccatgcctaa aagtatcaaa atcgaaatgc ttcaggaaac
     2281 cgtccccaac ttaggcatca tcaccgccta caacgacatg gtttccgcac accagccgtt
     2341 taaagacttc cctgaccaaa tcaaagacga agcgcagaaa aacggcgcga ccgcccaagt
     2401 cgccggcggc acgcccgcca tgtgcgacgg catcacgcaa ggctacgccg gcatggaatt
     2461 gtcgctgttc tcccgcgacg tgattgccat gagtaccgcc atcgggctgt cgcatcaaat
     2521 gtttgacggc agcctgttta tgggcgtatg cgacaaaatc gttccaggtt tgatgatagg
     2581 cgcgctttcg ttcggtcata ttccgggtat cttcgtcccc gcaggcccga tgtccagcgg
     2641 tatcggcaac aaagaaaaag cccgcacccg ccagcttttc gccgaaggca aggtcggacg
     2701 caacgaactt ttgaaaagcg aaatgggttc ttaccacagc ccgggcacct gcactttcta
     2761 cggcacggca aactccaacc aaatgatgat ggaaatgatg ggcgtgcacc tgcctgccgc
     2821 cgccttcgtc cacccttaca ccgacctgcg cgaagcgctg acccgctacg ccgccggaca
     2881 cctcgcgcgc ggcatcaaaa acggcacgat taaacctttg ggcgaaatgt tgaccgaaaa
     2941 atcctttatc aacgccttga ttggcctgat ggcaaccggc ggttcgacca accacaccat
     3001 gcacctcgtc gctatggcgc gtgcggccgg cgtgattttg aactgggacg acttcgacga
     3061 aatttcctcc atcatcccgc tgctcatccg cgtttatccg aacggcaagg ccgacgtgaa
     3121 tcactttacc gcagcgggcg gactgccttt cgttatccgc gaattgctga atgcaggcct
     3181 gttgcacgac gatgtcgata ccgtcgtcgg acacggtatg cgccactaca ccaaagagcc
     3241 tttccttatc gacggcaaac tcgaatggcg cgaagccccc gaaaccagcg gcaacgacga
     3301 catcctgcgc aaagctgaca acccgttctc ccccgacggc ggtctgcgcc tgatgaaagg
     3361 caacatcgga cgcggcgtga ttaaagtgtc cgccgtgcgc gaaggctgcc gcattattga
     3421 agcgcctgcc atcgtgttca acgaccaacg cgaagtgttg gctgcgtttg aacgcggcga
     3481 gttggaacgc gattttgtgt gcgtcgtccg ctaccaaggc ccgcgtgcca acggtatgcc
     3541 cgaattgcac aaactgaccc cgcctttggg catcctgcaa gaccgcggct tcaaagtggc
     3601 gctgctgacc gacggccgta tgtccggcgc gtccggcaaa gttccagcct ccatccacat
     3661 gacacccgaa gccctgatgg gcggcaacat cgccaaaatc cgtaccggcg acctgatccg
     3721 cttcgactcc gttagcggcg aactcaacgt cctgattaac gaaaccgaat ggaatgcccg
     3781 cgaagtcgaa agcatcgact tgggcgcgaa ccaacaaggc tgcggccgcg aactcttcgc
     3841 caacttccgc agcatgacca gcagcgcgga aaccggtgcc atgagtttcg gcggcgaatt
     3901 tgcctgatgc gcgtttcaga cggccttttc agaccgaagg ccgtctgaaa aattattcaa
     3961 gcgttttaag atagacgtag gttggattct cgaatccgac acagccgtcc aagatgtcgg
     4021 tttcttgaat ccgacctaca acctgtccca tcttaataaa ataccccatt ccacccggag
     4081 aaccgaaatg tccaaactga ccccccgcga aattttgacc gccggcgcag ttgtgccggt
     4141 aatggcgatt gacgacttaa gcaccgccat cgatttgtcc cacgcccttg tcgaaggcgg
     4201 catccctacc ctcgaaatca ccctgcgcac ccctgtcggc ctcgatgcca tccgcctgat
     4261 tgccaaagaa gtgcccaacg ccatcgtcgg cgcaggtacg gtaaccaatc ccgaacagct
     4321 caaagccgtc gaagacgcag gcgcggtttt cgccatcagc ccggggctgc atgaatccct
     4381 cgccaaagcc ggccacaaca gcggcatccc cctgattccc ggtgttgcca ccccgggcga
     4441 aatccaactg gctttggaac acggcatcga caccctcaaa ctcttccccg ccgaagtcgt
     4501 cggcggcaaa gccatgctca aagccctgta cggcccttac gccgatgttc gcttctgccc
     4561 gacaggcggc atcagcctcg ccaccgcgcc cgagtacttg gcactgccca acgtcctgtg
     4621 cgtcggcggc tcttggctga caccgaaaga agccgtgaaa aacaaagact gggacaccat
     4681 cacccgcctc gccaaagaag cggcggcgtt gaaacccaaa gcctgattcg catcgtaaaa
     4741 atgccgtctg aaaaaccttt cccgtttcag acggcatttt gccgattgag ggcacagtcg
     4801 gcatacacgg cagcactgat cagacatacc gcccctaaaa tgcccatccg ccttccgcat
     4861 aataaaaata acgttcagtt cattcgacag cagccggaca gcccatacta cgcggctgaa
     4921 aaaatgccgt ctgaaacgca ttcagacggc atccacttaa aaaaaacaac tgattcaacg
     4981 ccgattaatc cgcttccaaa accactttca tcacttggtt ttcggcggcg tgtttgaaca
     5041 cgtcgtaggc tttttccaat tcactgaatt tgaaatgatg ggtcagcatt ttggtgtaat
     5101 cgacggagct gctggaaatc gccttcatca gcatttcggt ggtattggcg tttaccagac
     5161 cggtagtgat ggcaagcttt ttaatccaga gtttttccag tttgaaatca acggattgac
     5221 catgtacacc aaccacagcg atatggccgc cgggtttcac aatgtcttgg cacatattcc
     5281 aagttgcagg gataccgacc gcttcgatgg cacaatctac gccgtcttcg ccgacgatgg
     5341 cgaaaacttg tttggagacg tcgccggaag cggggctgat ggtatgggtc gcgcccaact
     5401 ctttcgccag tttcaaacgg ttttcgtcca tatcgcacac gataatggcg gcggggctgt
     5461 acagttgggc ggtcaacaag gcggacatac cgacaggagc ggcacctgcg atgaatacgg
     5521 tgtcgccggg tttgacatcg ccgtattgca cgccgatttc gtgtgcggtc ggcaaagcgt
     5581 cgctcaacaa cagggcgact tcttcgttga cgttgtcggg cagcggcacg aggctgttgt
     5641 cggcataagg cgtgcggacg tattctgcct gcgtgccgtc aatcatgtag cccaaaatcc
     5701 agccgccgtt gcggcaatgc gaataaagtt ggattttgca gttgtcgcaa gtacagcatt
     5761 tgctgacgca tgaaataatg actttatcgc cgactttgat gttttttaca gcctcgccga
     5821 cttcttctac aataccgatg ccttcatgac cgagaatacg gccgtcggcg acttcgggtt
     5881 tttttgcctt tccaaatacc caaatcggta ccgcaaatcg tggttttgac gattttcacc
     5941 accgcatcgg tcggatcgat aatctgcgga cggggttttt cttcaaaacg gatgtcgttt
     6001 gcgccgtgat aaaccattgc tttcatgctg atactccttg cttgttgata aataatttca
     6061 ataccgcaat aaagtttctt tatatgagtt atatgcccct acaaaaaata agtaaataag
     6121 aattattttc acaatgttat acaataacat actgttttaa atataaataa aaccaccgat
     6181 tgatattaat gaacacaccc atccccttct ccgaacggct catccgctgg caaaaacaac
     6241 acggtcgcca ccacctccct tggcaggtca aaaaccctta ttgcgtctgg ctttccgaaa
     6301 tcatgctcca gcaaacgcag gtcgccaccg tgttggacta ctatccgcgt ttcttagaaa
     6361 aattcccgac tgttcaaata cttgccgccg cgccgcaaga cgaggtttta tccttgtggg
     6421 caggcttggg ctattacagc cgcgcgcgca atctgcacaa agccgcgcaa caagtcgtcg
     6481 gacaattcgg cggcacgttt ccgtcggagc gtaaagactt ggaaaccctc tgcggcgtag
     6541 gcagaagcac cgccgccgcc atttgcgcct ttgccttcaa ccgccgcgag accattttgg
     6601 acggcaacgt caaacgcgta ctctgccgtg tctttgccca agacggcaat ccgcaagaca
     6661 aaaaatttga aaacgcgctc tggacacttg ccgaaagcct gctgccgtct gaaaacgccg
     6721 atatgcctgc ctatacacaa ggtttgatgg atttgggcgc gaccgtgtgc aaacggacga
     6781 aacccttgtg ccaccaatgc ccgatggcgg acatctgcga agcgaaaaag caaaaccgca
     6841 ccgccgagct gccgcgcaaa aaaaccgccg ccgaagtacc gaccctgccg ctttactggc
     6901 tgattgtccg caaccgggac ggcgcgattt tgctggaaaa acgccccgcc aaaggcattt
     6961 ggggcgggct gtattgcgtg ccgtgttttg aaagtttgaa cgggctttcc gactttgccg
     7021 ccaaattctc cctgaccatg gcagatatgg acgaacaaac cgccctgacc caccgcctga
     7081 cgcaccggct gctattgatt acgccctttg aagcacaaat gccgtctgaa agcccttcag
     7141 acggcatttg gataaagccg gcgcatttga aagattacgg cctgcccaag cctttggaaa
     7201 tttatttaaa cggtaatagg ttagaataaa caaaataaac ccattgaact gttgtttgca
     7261 ggtatcgcag caagaacaac cgatgaattt gggtcgtatt ttaggcggcg ggataatgtt
     7321 caaatgggac atttggaacg gaagaagtcg gcaatttaaa aaggatttaa aaagcaaaga
     7381 aggtcaaaaa catgaacaca aacttaaatg acaaagacaa agccatggat accgcaatca
     7441 ggtttcagaa aaggatgagg attccgaaat ttttcttttt aattctctaa atcacaatgg
     7501 ttttggcatt tatccaagac gtgataacgg gttctaattt tctgcaaata acaattaatg
     7561 taaaattttc gtaaaaattt atcggctttt aaaacaaaat tgactaaaat agtcgcgagt
     7621 ttttactgca ataaaggaga ttgcaatgaa tatgaaaacc ttattagcac tagcggttag
     7681 tgcagtatgt tcagttagtg ttgcgcaagc acacgagcat aatacgatac ctaaaggtgc
     7741 ttctattgaa gtgaaagtgc aacaacttga tccagtaaac ggtaacaaag atgtgggtac
     7801 agtgactatt actgaatcta actatggtct tgtgtttacc cctgatttac aaggattaag
     7861 cgaaggctta catggtttcc acatccatga aaacccaagc tgtgagccaa aagaaaaaga
     7921 aggtaaattg acagctggtt taggcgcagg cggtcactgg gatcctaaag gtgcaaaaca
     7981 acatggttac ccatggcaag atgatgcaca cttaggtgat ttacctgcat taactgtatt
     8041 gcatgatggc acagcaacaa atcctgtttt agcaccacgt cttaaacatt tagatgatgt
     8101 tcgcggtcac tctattatga tccacacggg tggtgataat cactccgatc atccagctcc
     8161 acttggcggt ggcggcccac gtatggcatg tggcgtgatt aaataattcg attgttcgaa
     8221 acgaaaagtg cggtgaattt tgaccgcact tttttgctag atatttag
//
LOCUS       DAWISC010000084         8248 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWISC010000084 DAWISC010000000
VERSION     DAWISC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.084"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WIW87_001298"
     CDS             complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WIW87_001298"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ5174456.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1273..1878)
                     /locus_tag="WIW87_001299"
     CDS             complement(1273..1878)
                     /locus_tag="WIW87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174457.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WIW87_001300"
     CDS             complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WIW87_001300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ5174458.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2863..3159
                     /locus_tag="WIW87_001301"
     CDS             2863..3159
                     /locus_tag="WIW87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174459.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3173..3841
                     /locus_tag="WIW87_001302"
     CDS             3173..3841
                     /locus_tag="WIW87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5174460.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WIW87_001303"
     CDS             complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WIW87_001303"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ5174461.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERIYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6271..7134)
                     /locus_tag="WIW87_001304"
     CDS             complement(6271..7134)
                     /locus_tag="WIW87_001304"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ5174462.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDENGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7335..8198
                     /gene="rfbD"
                     /locus_tag="WIW87_001305"
     CDS             7335..8198
                     /gene="rfbD"
                     /locus_tag="WIW87_001305"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ5174463.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFIR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGSKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1985 a         2389 c         1903 g         1971 t
ORIGIN      
        1 atttccctca agcgatgcgg cgacaccaac acctgattaa aacacaaccg gcgttttcgg
       61 catatatgca aaaacgcatt ttccggcacg gcgttacacc gataccgaaa aatgcgtctg
      121 aatgtttcga caatccgatt aaagcattat gccaaatctg caaaaagtgg cgtagagaga
      181 tagcgttcgc cataagaagg cagcagcacg actatcagct tgccttcgtt ttcaggctgt
      241 ttggcaagct gcaacgcact ccaaaccgcc gcaccggaag aaatacccac caaaatgcct
      301 tctttttccg ctattgcgcg ggcggtttca aaagccgctt cgttcgacac tttggtaatg
      361 ctgtcgtaaa ttttggtatt caaaacggtc ggaataaagc ctgcgccgat gccttgaatc
      421 gggtgcgggc ctttttcgcc gccgctcaat acgggtgaag cttcaggctc gacggcaacc
      481 actttaactt cgggtttgta ttttttcaac acttcgccca cgccggtaat cgtaccgccc
      541 gtgccgacgc cggcaacgaa gacatcgact ttgccgtccg tatcccgcca aatttcctcg
      601 gcggttgttt tgcggtggac ttcggggttt gcctcattgt cgaactggcg cggcataaaa
      661 taagtgtcgg gatgcgcgtc caccaaggat ttcgctttgg caatcgcgcc cgccatacct
      721 tcggcggcag gggtcagaat cagctccgca ccaaacgcgc gcaacagcat tttgcgctcc
      781 ttactcatgc tttccggcat ggtaatcgcc agcttgtagc cgcgtgcggc acataccatt
      841 gccaaaccga cacccgtatt gccgctggtt gcttcgacaa tgacggtgtt tttgttgatt
      901 ttgcccgctt tttcggcacc ctcaatcatt gcttcggcaa tgcggtcttt gacgctgctg
      961 cccggattga aaaattccag tttcacggca acctctgcct tcaaaccttc ggtcagacgg
     1021 ttcagtttga ccaaaggcgt gttgccgatt agttcggtaa tgctgtttgc aattttcatc
     1081 ttctgctcct gaataataag gtttcgacag taaggcgcaa tccccatagg catcctgtcc
     1141 gattgcaggg gatttgcgcc gaaacataaa caaatttcgg atatggcttt atcctaaacc
     1201 tatgccgcct aaaaaaccaa tatccaaaat ttctttcttt ataacttttt gttccgatac
     1261 ggccgcaccg tattatcttc caatttctga aagcagcagc accagtaccg agacctgcat
     1321 caacgctgcc gtcaacccgc cgagtcgcag ctgcgagccg ccgaacgttg acggcggctg
     1381 ccggtctata ccgcagcggt ggacataggc aaccgccatc agcacaaact gcaacacgaa
     1441 ccaataaacc ggcttcagcg cataaagttt gagcagtgcc gcaaacacaa acacttgaac
     1501 cgttcccgaa aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga
     1561 tacattactg acggcaaaaa gcccgagcag cggatgttcg gggtctgcct gccatccgtt
     1621 tccatctgtt ttccggttcc aatgcccgat gaaaaaaaat atgctgccca aagtcaggta
     1681 aaaatggggg atgaacaagg gcgcggcgcg ggtcattccc catatgccgg gcatcagctt
     1741 tgcccccaaa accgatatgc ccagccacag cataatactc gcccaaaacc attgcaacat
     1801 tcctgcgcgg aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc
     1861 caaatgcagc aaaatcatga atacgccaac tcaccttcaa acaccgcttc cgcagggccg
     1921 gtcatcatca catcgccgcc gcaggcccat tcgatatata aagtcccgcc cggcaaaacc
     1981 acctctaccg ttttcccttc atccaacagc cccagacgga tacccgccac cacagccgca
     2041 cacgcgcccg taccgcaagc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacga
     2101 atcgcggttc ggccgacaac ctgcataaag ccgacattga cgcgttcggg aaactgcctg
     2161 tgcggttcga taagcgaacc ggtttcgcgc accggcgcgc attccacgtc atcgaccaca
     2221 atcaccgcat gggggttgcc catattgacg cagctgacag gctgaatgcc ggattcgaga
     2281 tgcaccccgt aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca
     2341 gacggcataa acttcggttt gcccatattg accgtaacca taccgttatc ggacaatttc
     2401 ggaaaaataa cgccatttgc cgtttcaaca caaatttctt tcttatcggt caaacccttg
     2461 tctgcaacaa aacgggcaaa acaacgcgct ccgttgccgc attgcccgac ctcgctgccg
     2521 tcggcattga aaatacggta acggaaatcc acgccttcgg tttccgaacg cccgaccacc
     2581 aaaagctggt cgaagcccac gccccggaag cggtccgccc attccgcaat cggcgcgtcc
     2641 tcgggggtaa aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc
     2701 attttggtaa atttcaaagt gttcatattg ttccgaaatc ccgtatcgtc tgttgcttcc
     2761 gaatccgaaa tcccccgcac cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa
     2821 aatcagccat tatactgttt gttacccctt acaagaaaca ccatgttccg catattactg
     2881 ttgcttttcc cttgtgcaat ccttgccgcc tgcacccgaa acataccggc aaacccggcc
     2941 gtttcaaatt gcgcgcatct tcaaaaccgc accatcctgt attttgacgc acacggcaaa
     3001 cccgccgacg gcgcagtaaa gggcggattt tacaaaatta tcgaaataca gggtgcaaac
     3061 tgcatcctta ttcaagactt taacagcacg ggcaatgccc tcggcggatc ctatgtgccg
     3121 gactgccgca aatcatccct tttcccgaaa aatatttgac ttcaaactgc ctatgaacca
     3181 cgacatcact ttcctcaccc tgttcctact cggtttcttc ggcggaacgc actgcatcgg
     3241 tatgtgcggc ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgctt
     3301 ttggctgatc ctgctgctta acacaggacg ggtaagcagc tatacggcaa tcggcctgat
     3361 actcggatta atcggacagg tcggcgtttc actcgaccaa acccgcgtcc tgcagaatat
     3421 tttatacacg gccgccaacc tcctgctgct ctttttaggc ttatacttga gcggtatttc
     3481 ttccttggcg gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc tgaacccgat
     3541 actcaaccgg ctgttaccca taaaatccat acccgcctgc cttgcggtcg gaatattatg
     3601 gggctggctg ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg gaagcggtag
     3661 tgcggcaacg ggcgggttat atatgcttgc ctttgcactg ggtacgctgc ccaatctttt
     3721 agcaatcggc attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct
     3781 gtgtacggga ttatccgtat cattatgggc attatggaaa cttgccgtcc tgtggctgta
     3841 agccccattc attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggcact
     3901 ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt
     3961 tgatggacag acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct
     4021 gaccgacttg caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt
     4081 gtaccaaacc gtctttgccc ggcatcacgc tgacaatcgc gccgacattg ttatcgagga
     4141 ttttcaccac agtgccttcg tacactttgc ccacttccac ttcggcagta atctgctcga
     4201 tgcgtttttt cgccgcatcg ccggcttctt gagtggttgc ggcaatggta atcgtaccgt
     4261 cttcggcaat attgatttcc gtaccggttt cagcggtaat cgaacggatg gtttcaccgc
     4321 ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtgaac aagcgtggcg
     4381 cgtgtgcgga cagctcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca
     4441 gacgcgcttc tttggcctgt gccaaagcga tttgcataat ttctttggta atgccttgga
     4501 ttttgatgtc catttgcagc gcggtaacgc cttcggtcgt accggccact ttaaagtcca
     4561 tatcgcccaa gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat ttgttgcctt
     4621 ccagaatcag acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca
     4681 gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg gattcggtaa
     4741 tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca
     4801 atgcacgttt agccaaacgg ccgtgaccga tttcacggcg tttcggtgcg cccatgcggc
     4861 ccacttcgcc ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtgtatt
     4921 cgccggacag cgcgtcgatg atttgctcgt cgcgcgaagt acccaaagtt gcaacggcca
     4981 aagcttgggt ttcgccacgg gtaaacaatg cagaaccgtg cgtgcgcggc aatacgctgg
     5041 tttggatgtt cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttggccat
     5101 ccaaaatttg gctgcggacg acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt
     5161 tggctgccaa agtgtcggtt tcttcggtaa tcaaggcttc ttttaccgca ctccaagctt
     5221 cgtccaattt ggcagaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt
     5281 cgccggcaat cccgcggact ttggcaacca gttcctcatt ggtttcaggt gctttccaat
     5341 cccaaagttc cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca
     5401 tttgatcgtg gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt
     5461 cggattccac catcaacacg gcttttgaag taccggcgac caccaagtcc aattgcgatt
     5521 tcgccaattc ggctttagtc ggattcaaaa cgtacacgcc gtttacataa ccgacgcgtg
     5581 ccgcgccgat cgggccggca aacggtacgc cgctcaacac cagcgcggca gatgcaccca
     5641 acattgcagg aatatcagaa tcgatttcag gatcgacgga cacgaccatc gctacgattt
     5701 ggatgtcgtg gtagaaacct tcagggaaca gcggacgaat cggacggtcg atcagacggc
     5761 tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg ccgggaattt
     5821 tgcctgcggc gtaaatgcgt tccaaataat cgacggtcag ggggaagaag tcttgacctt
     5881 ctttcacttc tttgttggtg gtaacggcaa ccaaaacaac ggtgtcgccc atagagactt
     5941 taacggcagc ggcggcttgg cgggcaattt cgccggtttc caaagtaacg gtctgattac
     6001 cgtattggaa ggtcttaacg tgtttgtcga acatcattgt tcctttcaaa ataccgcact
     6061 gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt
     6121 gcggcaggtt ataaacaagc tttcagacgg catttcggac gctgaaagca attgcggatt
     6181 ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaccata
     6241 tttctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc
     6301 ccgatacttc tcgacagttt tctgaatcac atcggcaggc actttcggcg caggggcttt
     6361 tttgttccaa ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg
     6421 attggtgccg actttgtatt ggtcggcagg ccaaaaacgg ctcgaatcgg gagtcaatac
     6481 ctcatccatc agcgtcagcg taccgttttc atccaaaccg aattcaaatt tggtatcgca
     6541 aataataata ccgcgcgatt tggcatattc cgccgcttcg gtgtaaagcc gaaccgcctt
     6601 ggcgcgcact tcttccgcca attctttgcc gataatgcgt ccgcattctt caaagctgat
     6661 gttttcatcg tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag
     6721 ttgttgcgct tcctgcatac cttcaggcag ttgaataccg caaaccgagc cggttttttg
     6781 ataatctttc caaccgctgc ctgccagata accacgcaca atcgcctcta ctttcaccgg
     6841 agtgagcttt ttagccacga cggcgcgttt ctctaaagct ttggcttcgt tttcaggcaa
     6901 aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcgcca gttttttaaa
     6961 ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat
     7021 cacatcaaac gcggacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc
     7081 atataaatcg cgcacttttc caaaatagat ctttaccaaa ccaatctcac tcatttcgcc
     7141 ccccccctga aaatatcttg aaaataccga cccgacaccc gacaggtttg aatcacaaac
     7201 cgatattcta gccgaagtcg gcgcaaaaca atacccatgg cacaaaaagc caacccgtca
     7261 accgtcggca aaattttggc actataatac cgacagcaag tcctacaata cacttttacc
     7321 aaaggaaata cctcatgaga atcctattga caggctcgaa aagccaactg gcacgctgcc
     7381 tgcgcgaccg tcttccggaa gactgggaaa ccattgcgac ggattccgca tccctagaca
     7441 ttaccgatgc cgatgccgtc tgcaacatgg tcaaaagttt ccaacccgac gccattgtca
     7501 acacggctgc ctatactgcc gtcgacaagg cggaaggcga tgcggcagcg gcatttgccg
     7561 tcaatgcttc cgccgtttac aaccttgcct tggcagcaca tcgcgcccat gcccgattca
     7621 tccacatctc aaccgactat gtctttgacg gtaaagggaa aagaccctat caggaaagcg
     7681 actttaccaa tccttccaat gtatacggac aatccaaaac cgcaggcgag ctgctcgcac
     7741 tgtctgccaa tcccgacagc cttatcctgc ggacttcttg gctgtttagc gaatacgggg
     7801 acaactttat ccgcacgatg ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc
     7861 acaaccaaat cggctgcccg acctatgccg gcgacttgtc cgccaccatc atccgcctgt
     7921 tgcagcactc caatcccgtt cgcggcattt accactacgc cggcagcaaa tccgtatcct
     7981 ggtacgaatt tgcccaacat attttccaag cggcatcgca acagcagaca tccttccccg
     8041 ttcccgaatt gactgccgtt tcagacaagg aatatccgac cgccgccccc aggcccgcat
     8101 acagcatttt ggactgccgc aaaatcgaaa acgacttcgg catcaaaccg tcagactggc
     8161 aaaaagccct tgcacaggtc gtttccaagc tgctctgatg ccgcccgccc ctctgtttcc
     8221 gccgtcaagc accgccttgg cggttttc
//
LOCUS       DAWISC010000085         8185 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWISC010000085 DAWISC010000000
VERSION     DAWISC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.085"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            299..1468
                     /gene="prpF"
                     /locus_tag="WIW87_001306"
     CDS             299..1468
                     /gene="prpF"
                     /locus_tag="WIW87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ5174464.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1882..3081
                     /locus_tag="WIW87_001307"
     CDS             1882..3081
                     /locus_tag="WIW87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ5174465.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3440..4060
                     /locus_tag="WIW87_001308"
     CDS             3440..4060
                     /locus_tag="WIW87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ5174466.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(4187..4630)
                     /locus_tag="WIW87_001309"
     CDS             complement(4187..4630)
                     /locus_tag="WIW87_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ5174467.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4804..5172
                     /locus_tag="WIW87_001310"
     CDS             4804..5172
                     /locus_tag="WIW87_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5174468.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5217..6371
                     /locus_tag="WIW87_001311"
     CDS             5217..6371
                     /locus_tag="WIW87_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ5174469.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6428..7300)
                     /locus_tag="WIW87_001312"
     CDS             complement(6428..7300)
                     /locus_tag="WIW87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ5174470.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDQASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7385..>8185
                     /locus_tag="WIW87_001313"
     CDS             7385..>8185
                     /locus_tag="WIW87_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ5174471.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSCEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVL"
BASE COUNT         1787 a         2225 c         2368 g         1805 t
ORIGIN      
        1 tttaatccac tataaattta atccactata ctgtaaatcg tcattcccgc gcaggcggga
       61 atccagaaag tggaattgag gaaacctttt tatccgatga gtttctgtgc ggataaatct
      121 ggattcccgc ctgcgcggga atgacggggt ttaataatct gccgtatcac aacacagtag
      181 ccgtagattg gggcgaaccc cgacagtttg cggaatcaaa cggctttggt cggagtggca
      241 gcctaatcca ctataaaaat cgtgggcaga gcccacgcta cataaggaga atctagaaat
      301 gccgcaaatt aaaattcccg ccgtttacta ccgtggcggt acatcaaaag gcgtgttttt
      361 caaacgttcc gacctgcccg aggcggcgcg ggaagcggga agcgcacgcg acaaaatcct
      421 cttgcgcgta ctcggcagcc cggatcccta cggcaagcag atagacggtt tgggcaacgc
      481 cagctcgtcc accagcaagg cggtgatttt ggacaagtcc gaacgcgccg atcacgatgt
      541 cgattacctt ttcgggcaag tttccatcga caaacctttt gtcgattgga gcggcaactg
      601 cggcaacctc accgctgccg tgggcgcatt ctccatcgaa cagggcttgg tcgataaagg
      661 caagattcct tcagacggca tctgcacggt caaaatctgg cagaaaaaca tcggcaaaac
      721 cattattgcc catgtaccga tgcaaaacgg cgcagttttg gaaacaggcg attttgagct
      781 cgacggcgta acgttcccgg cagccgaagt acaaatcgaa tttcttgatc cagccgacgg
      841 cgaaggcagt atgttcccaa ccggcaattt ggtcgatgaa attgatgtgc cgaatatagg
      901 ccgtttgaaa gccacgctca tcaacgcggg cattccgacc gttttcttga atgccgccga
      961 cttgggctac acaggcaaag agttgcaaga cgacatcaac aacgatgccg cggctttgga
     1021 aaaattcgag aaaatccgcg cttacggtgc gctgaaaatg ggtctgatca gcgacgtatc
     1081 cgaagctgcc gctcgcgcgc acacgccgaa agtcgccttc gtcgcgcccg ccgccgatta
     1141 caccgcctcc agtggcaaaa ccgtgaacgc cgccgacatc gatttgctgg tacgcgccct
     1201 gagcatgggc aaactgcacc acgcgatgat gggtaccgcc tctgttgcca ttgcgaccgc
     1261 cgccgccgta cccggtacgc tggtcaacct tgccgcaggc ggcggaacgc gtaaagaagt
     1321 gcgcttcggg catccttccg gcacattgcg cgtcggtgca gccgccgaat gtcaggacgg
     1381 acaatggacg gccaccaaag cggtcatgag ccgtagcgca cgcgtgatga tggaaggttg
     1441 ggtcagggtg cctgaggatt gtttttaaat tgacgtagca tgggtttgcc cgcgagccat
     1501 aaaaaggtcg tctgaaaaac aagtaaacat caaatcactg accattcctt tcccttgccc
     1561 tgtggcggaa ggcggcaaat cacaaggaag aacacggaaa ccccgataaa agacagcttc
     1621 ccgtattacc gtcattcccg cgcaggcggg aatccagacc tgtcaatatg gaggattggc
     1681 aggggaaaac aggtttcgtg agttctacat tctggattcc cgccacagcc tgtcctcgcg
     1741 taggcgggga cggaataacg atagaaaatg cggcatacgc tttgcccaaa gaggccgtct
     1801 gaaacacctt gcgcctgatg tctgcctttt tcagacgacc ccacaccaaa aaaacaacca
     1861 caaactacaa ggagaaacat catgtccgac caactcatcc tcgttctgaa ctgcggcagt
     1921 tcatcgctca aaggcgccgt tatcgaccga aaaagcggca gcgtcgtcct aagctgcctc
     1981 ggcgaacgcc tgaccacgcc cgaagccgtc attacgttca acaaagacgg caacaaacgc
     2041 caagttcccc tgagcggccg aaattgccac gccggcgcgg tgggtatgct tttgaacgaa
     2101 ctggaaaaac acggtctgca cgaccgcatc aaagccatcg gccaccgcat cgcccacggc
     2161 ggcgaaaaat acagcgagtc tgttttgatc gaccaggccg taatggacga actcaatgcc
     2221 tgcattccgc ttgcgccgct gcacaacccc gccaacatca gcggcatcct tgccgcacag
     2281 gaacatttcc ccggtctgcc caatgtcggc gtgatggata cttcgttcca ccaaaccatg
     2341 ccggagcgtg cctacactta tgccgtgccg cgcgagttgc gtaaaaaata cgctttccgc
     2401 cgctacggtt tccacggcac cagtatgcgt tacgttgccc ctgaagccgc acgcatcttg
     2461 ggcaaacctc tggaagacat ccgcatgatt attgcccact taggcaacgg cgcatccatt
     2521 accgccatca aaaacggcaa atccgtcgat accagtatgg gtttcacgcc gatcgaaggt
     2581 ttggtaatgg gtacacgttg cggcgacatc gatccgggcg tatacagcta tctgacttcc
     2641 cacgccggga tggatgttgc ccaagtggat gaaatgctga acaaaaaatc aggtttgctc
     2701 ggtatttccg aactttccaa cgactgccgc accctcgaaa tcgccgccga cgaaggccac
     2761 gaaggcgcgc gcctcgccct cgaagtcatg acctaccgcc tcgccaaata catcgcttcg
     2821 atggctgtgg gctgcggcgg cgttgacgca ctcgtgttca ccggcggtat cggcgaaaac
     2881 tcgcgtaata tccgtgccaa aaccgtttcc tatcttgatt tcttgggtct gcacatcgac
     2941 accaaagcca atatggaaaa acgctacggc aattcgggca ttatcagccc gaccgattct
     3001 tctccggctg ttttggttgt cccgaccaat gaagaactga tgattgcctg cgacactgcc
     3061 gaacttgccg gcatcttgta gccaaaaaag ggacgagtcc gcaaaaatgc cgtctgaaac
     3121 cccaaacgcc cgattaggct gatgaggatt ttagacggca ttgttcattt ttttgttatc
     3181 ttgcattttt gtgcggacgg tggaatttca tcctgtaaac ataaatattt gtcggaaaac
     3241 agaaaccctc cgccgccatt tctacgaaag caggaaacca gcaacgcaaa gcgacaggga
     3301 tttgttggaa atgaccgaaa ccgaacgaac cggattcccg cctgcgcggg aatgacggga
     3361 ttttctgttt ttgtggaaat gacgggattt tgaatttcgg gcgtacaata cggaaaacat
     3421 gacgataagg aaacaaacca tggcacagtt tttcgctatt catcccgaca atccccaaga
     3481 acgcctcatc aagcaggcgg ttgaaatcgt caataaaggc ggcgtggtcg tttatccgac
     3541 cgattcctgt tatgccttgg gctgcaaact cggcgataag gcggcgatgg aacgcatact
     3601 ctccatccgc aaaatcgatt tgaaacacca cctgaccctg atgtgcgcag atttgagcga
     3661 gttgggcaca tacgccaaag tcgacaacgt acagtttcgt cagcttaaag ccgccacacc
     3721 cgggccttat acttttattt tacaggcgac gaaggatgtg ccggcgcgca cgctgcaccc
     3781 gaaacgcaaa accatcgggc tgcgtattcc cgataatgcc attgcacaag ccctgctggg
     3841 ggaattgggc gagccgcttt taagctgcac cctgatgctg cccgaagacg gcgaaccatt
     3901 gaccgatcct tatgaaatcc gcgagcgttt ggaacacgcc gtcgatttgg tgattgacgg
     3961 cggctggtgc ggaaccgagc cgaccaccgt cgtcgatatg accgacggca cggaattggt
     4021 gcgccaaggt tgcggcgata cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa
     4081 gcatcggctg ttcagacggc attgcgcgcc ttgccggcgg cagtccgaaa tgccggcgcg
     4141 tatcgcgctc ggtcggaata tccgtttgaa acggcatttt gatgcattac tgcaccgcaa
     4201 tcggaattct cggttcgtag agcaggtcgt aggtcggctt gttgagcagg tcttggagcg
     4261 tgaaaccgtc cagatacgtg aaaaacgact tcatcgcgcc gccgagtatg cccgtcagcc
     4321 ggcaggacgg tgtaatcagg cattcgttgt tctcgcccat gcactcgacc agctgcatcg
     4381 gttcgaggtg gcggacaacc gagccgatgt tgatgcggtc gggcggtgcg gcaagccgca
     4441 gaccgccgcc ttttccgcgc acactgtgga ggaagccgcc tttgaccagc gcggtaacga
     4501 ccttcatcag atggcttttg gaaatgccgt aggttacggc gatggtactg atgttgacca
     4561 gcgcatcgtc gttgatggca gtgtagataa ggacgcgcag cccgtagtcc gtatgttgtg
     4621 tcaaatacat gattttctcg gtatggattg ttattcttat cggtacggtt taaggttcac
     4681 ggacaatacc ttaatggttg aaaccctgtc cgtcggggcg gtagaatgca gcctgtctgc
     4741 ggcggtatgc cgtctgaaac atccgcgcta ccgtttgaga atttgttatt gtaactcaaa
     4801 atcatgaaac cgttgaaacg acatcccgcc cttatcgggc tttcgcgtga ccaccaccat
     4861 tcgctttccc tgtgcgtgcg tctgttgcgg acgccggaag aaaggcatcg ggacgaactc
     4921 gaaccgcatt tttccgaatt ggaaacccat tttcgcgaag aagaaaccaa gtttgcccca
     4981 atttggcaga atgtcgcccc cgaattgaaa caacgtttcg agaaagacca cgcccgactg
     5041 cggcagatga tggcaagccc cgaatacggt aacgcggcgt ggaataccgc ttttgccaca
     5101 accctgcgcg accacgcgcg ctttgaagaa cgcgagctgt ttcccgccgc cgaaccgttt
     5161 ttgccggcat gattccgttt tgcggtaaat atattaatga taaacaagga acacacatga
     5221 aatttaccaa gcaccccgtc tgggcaatgg cgttccgccc attttattcg ctggcggctc
     5281 tgtacggcgc attgtccgta ttgctgtggg gtttcggcta cacgggaacg cacgagctgt
     5341 ccggtttcta ttggcacgcg catgagatga tttggggtta tgccggactg gtcgtcatcg
     5401 ccttcctgct gaccgccgtc gccacttgga cggggcagcc gcccacgcgg ggcggcgttc
     5461 tggtcggctt gactatcttt tggctggctg cgcggattgc cgcctttatc ccgggttggg
     5521 gtgcgtcggc aagcggcata ctcggtacgc tgtttttctg gtacggcgcg gtgtgcatgg
     5581 ctttgcccgt tatccgttcg cagaatcaac gcaactatgt tgccgtgttc gcgctgttcg
     5641 tcttgggcgg cacgcatgcg gcgttccacg tccagctgca caacggcaac ctaggcggac
     5701 tcttgagcgg attgcagtcg ggcttggtga tggtgtcggg ttttatcggt ctgattggta
     5761 cgcggattat ttcgtttttt acgtccaaac gcttgaatgt gccgcagatt cccagtccga
     5821 aatgggtggc gcaggcttcg ctgtggctgc ccatgctgac tgccatgctg atggcgcacg
     5881 gtgtgttggc ttggctgtct gccgtttttg cctttgcggc aggtgtgatt tttaccgtgc
     5941 aggtgtaccg ctggtggtat aaacccgtgt tgaaagagcc gatgctgtgg attctgtttg
     6001 ccggctatct gtttaccgga ttggggctga ttgcggtcgg cgcgtcttat ttcaaacccg
     6061 ctttcctcaa tctgggtgtg catctgatcg gggtcggcgg tatcggcgtg ctgactttgg
     6121 gcatgatggc gcgtaccgcg cttggtcata cgggcaatcc gatttatccg ccgcccaaag
     6181 ccgttcccgt tgcgttttgg ctgatgatgg cggcaaccgc cgtccgtatg gttgccgtat
     6241 tttcttccgg cactgcctac acgcacagca tccgcacctc ttcggttttg tttgcactcg
     6301 cgcttttggt gtatgcgtgg aagtatattc cttggctgat tcgtccgcgt tcggacggca
     6361 ggcccggttg agacaaaccg ccgcagattt cgggtctggg cggtttgctt ttcagacggc
     6421 agggcggtca gttgccgtcc agccagcggt cgcgtgtggt tttggcttct tcaaaatagc
     6481 ggtacagggc ttcgcggtcg tcggtggtca ggatgtttgc caaaacgtcc aactgtttgc
     6541 ccaagccttg aaccagttgc agcaggctgt ctttgttggc aaggcagatg tccgcccaca
     6601 cggcgggatg accggaggcg atgcgggtga agtcccgaaa gcccgtggcg gcgaatttca
     6661 gatattcctg tccgtcgggg tggtcgagaa tctggtggac ataggcgaag gcggtcaggt
     6721 ggggcatatg ggagacggcg gcgaaaaccg cgtcgtggcg ttgcgcgtcc atcgtataaa
     6781 tttccgcacc gaccgcgtgc cacaggtttt ctaccaaggc aatgccgtct gaatgttcgc
     6841 cgccgtgtgg cgtgatgatg agttttctgt ggcggaacag cccgaactgc gcggcttgcg
     6901 caccgcttct gtccgaaccg gcaattgggt gggcggcgat gcagtggtgc aggcggtcgg
     6961 gcagacagcg gcggaaggct tcgatgaccg aagatttggt gctgccgaca tcggaaatcc
     7021 aagtgtgttc cggcaaaacg gggcgcagcg cggtcaaaat ggcgggaacg gtggcgacgg
     7081 gcgtggcaat cagtaccaag tccgcaccgc cgatgctgtc cgcgtcgatg gcaacggaag
     7141 cctggtcaat cacgccgcgt tccaatgcac gttcgaggtt gtcgcggtcg gtgtcgatac
     7201 cggtaacggt gcggacgagt ccctgccttt tgaggtcgag aacgaacgaa ccgccgatca
     7261 gccctacacc gatgagggca atatggttca aaatgggcat ttgtgtaaac ggttttcgca
     7321 aagtaccgtc atggtagcct atcggcggaa tatgccgcaa ggtcggcagg aaaaaggaga
     7381 agaaatggac aaaatcagag ttgccgccgt gcagatggtg tcgggcgtgt cgccggaaac
     7441 caacgtcgcc gccatgaaac gcctggtcgc acgggcggcg gagcagggtg cggattgggt
     7501 gctgctgccc gaatattggg tgctgatggg cgcaaacgat accgacaaac tcgcgcttgc
     7561 cgagcctttg ggcggcggac gctttcagac ggcattgagc gaaacggcga aagaatgcgg
     7621 cgtggtgctg ttcggcggga ctgtgccgct gcaaagctgc gaggcgggta aagtgatgaa
     7681 tacgctgttg gtgtacggac gggacggcgt aaggacgggg ctgtaccaca aaatgcacct
     7741 cttcggtttt tccggtttgg gcgaacgcta tgccgaagcc gataccatcc gcgcgggcgg
     7801 ggatgtgccg cacttgtcgg cagaaggcgt gccggtggcg gcgggcattt gttacgatgt
     7861 ccgctttccc gaatttttcc gacgccagtt gccgtttgac gtattgatgc tgcccgctgc
     7921 gtttacgcac acgacgggca aggcgcattg ggagctgctg ctgcgcgcgc gtgccgtcga
     7981 aaaccaatgt tacgtcgtgg cggcggcaca gggcggtttg cacgaaaacg gacggcgcac
     8041 gttcggacac agcatgattg tcgatccgtg gggcgacgtg ttggacgtat tgcccgaggg
     8101 cgaaggcgtt gttacggcag acatcgatgc caaccgcctg aacagcgtcc gcaaccgcct
     8161 gcccgccttg aaataccggg ttttg
//
LOCUS       DAWISC010000086         8112 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWISC010000086 DAWISC010000000
VERSION     DAWISC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.086"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(246..974)
                     /gene="rmpM"
                     /locus_tag="WIW87_001314"
     CDS             complement(246..974)
                     /gene="rmpM"
                     /locus_tag="WIW87_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ5174472.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1338..2288
                     /locus_tag="WIW87_001315"
     CDS             1338..2288
                     /locus_tag="WIW87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ5174473.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2401..3135)
                     /gene="fnr"
                     /locus_tag="WIW87_001316"
     CDS             complement(2401..3135)
                     /gene="fnr"
                     /locus_tag="WIW87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ5174474.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3340..4761
                     /gene="hemN"
                     /locus_tag="WIW87_001317"
     CDS             3340..4761
                     /gene="hemN"
                     /locus_tag="WIW87_001317"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ5174475.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5114..6688)
                     /locus_tag="WIW87_001318"
     CDS             complement(5114..6688)
                     /locus_tag="WIW87_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ5174476.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6718..6911
                     /locus_tag="WIW87_001319"
                     /pseudo
     CDS             6718..6911
                     /locus_tag="WIW87_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7009..8109
                     /locus_tag="WIW87_001320"
     CDS             7009..8109
                     /locus_tag="WIW87_001320"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ5174477.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2048 a         2272 c         1995 g         1797 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc tgaatctgaa
       61 tccgaatccg aacctgcacc ccgattctga cgatagccgt ttcagaaaac cacccaaaaa
      121 aacaaacccc gcaaaagcgg gggtttgttt cacatcatcg atgccgaccg acgtttcacg
      181 aatattgcaa cagtacctgg tacaaaatcc actaacctca tgcatcggca agatattgcc
      241 tagccttagt gttggtgatg attgtgtgcc ggcacaacct gacgggttac gatgctgcgg
      301 attttcacat ccacacggcg gtcaggttcg atacatgcaa tcagagcctc acgttttttg
      361 gctttagaga ctttcgcacc cagtttggca acttcggctt cacaaacttg agtcatttgc
      421 gcttgagatt cgcccaagcc gacagcagaa attctagaaa caggtacgcc gttgctgacc
      481 aggttgtttg ccactacgta tgcgcggcgt tcggacaggg cctgattgta tttgtcagaa
      541 cccataaagt cggtatggcc ttcgacgcgg acagattgga cattggttcg actcaggcgt
      601 tgcgccaata ctttcaggtt gtcttgagct tcggcgcgca atgaatcctt atcgaaaccg
      661 aacagggttt tggcagacag ggaaatggtt tcatcaacat attgcggagc ctgctccaca
      721 acgacgacag gcgcgggtgc gggttcgggt tctggctcgg gttcgggggc agcaaccgca
      781 tcgccgcatt ctacgcgacc ttggcttgct ttatcaaagt aggcgttttt ccagcattcg
      841 ccatagttgt tgcgtacgat ttcgttcgac tggccgctta cggtgtaacc ctgaacggac
      901 gcctcgcccg caacagcagt gccggaagca agcaatgcaa cgaataatgc gcttaatttc
      961 agctgtttgg tcattttatt ccctcattaa atttgtacag cagatacaga agcaactgct
     1021 gaattgccaa catacaggcc gctgtagaaa catggcggca aatcggatat ttcagtctcc
     1081 actataaaga agcagggggc ggactgtcaa tactcaacgc ctgaaaacaa ccgtaaaaca
     1141 gcctgtttcc tgatagtcta tcatgccgaa aaatcaattc cgtgtcatta ctttaggggg
     1201 attttttcca tatcgcacga ttgccgttgc acaaaaacaa caaacacaac ggcaaagccc
     1261 cataccgtac cggcaagaaa atatgataaa ttataaacaa tgttcagcca cccgacacag
     1321 acccacaccg acccgccatg aaattacaac aattgaaata cgccttagaa gtttaccagc
     1381 acaacctgaa cgtttccgaa gcggccgaag ccttgtttac ttcgcaaccc ggcatctcca
     1441 aacaaatcaa attgctggaa gaagaaatcg gcattcagat ttttatccgc agcggcaagc
     1501 gcgtggtttc ggtctcgcag ccgggcaagg tggttttgga tattgcggaa cgtattttgc
     1561 gcgatgttca gaacattaaa aatatcggca gcgagtttac cggacaggac agcggttcgc
     1621 tgacggttgc cacgacgcat actcaggcac gctatgccct acccttgatt gttgccgatt
     1681 ttgtgaaacg ctatccgaaa gtcaacctga ccatcaaaca ggggagccct gccgccatcg
     1741 cacgtatggt tacctcaggc gaggcggact tggcgattgt tacggaacgg atagacgacc
     1801 atcccgaact gggaaaactt ccctgctatg actggactca tgcggttatc gtaccgaacg
     1861 accacccctt gctcgaatgc agaaaccccc tccgtattga agatttggcg aggtttccgc
     1921 tgattactta tgaatttgca ttcaatgcgg gcagcagcat cgcgcgggca ttttccaaag
     1981 cccgtttgga acaacccgat gtcgcattgg ctgcggcaga tacggacgta ttgaagactt
     2041 atgtgcgctt gggtttgggc gtgggactga tggcgaaaat ggcgtacaac ccggatacgg
     2101 acggcgattt gcagcttgtg gatgcggcac acctgttcga gccgtcgccg acgtggattg
     2161 ctttgcgcag cgatacttat ttgcgcggat atgcctacga ctttatccaa gcgttcgcgc
     2221 cgcacctgac acgcgagaag gtggatagga ttctgtacac gcccatcagc gaggattttt
     2281 cgatttaggc ggctgccggt tttcagacgg cactttgcgg cagatacaac aaacaggaca
     2341 gatgttttcg tctgccctgt gtttattgag aatgctgtct gaaatgttcg tacgggttaa
     2401 tcaaatggcg tgcgagcagc cggacaccat ttttttcaac acctgcagat tgaggatttt
     2461 gatgtgctta tgctcgacgg aaatcaatcc ttcctgatga aatttagata atgtgcggct
     2521 gacggtttca agtttcagcc cgagataact gccgatttct tcgcgggaca ttcttaagat
     2581 gaagtcgttg gcagcaaaac ctcgggaata aaggcgttgg gaaaggttca gcaggaaggc
     2641 ggcaatccgc tcttcggcgc gcatattgcc caacagcagc ataacacctt ggtcgcgcac
     2701 gatttcacgg ctcatcatgc ggaagaagtg cgtacgcagg ctggggatgt tttgccccag
     2761 ttcttcgatg tgggtaaacg gcagttcgca cacttcgctg tcttccaagg cgaccgcgtc
     2821 gcaactgtgc acatgggaac agatgccgtc catgccgatg agttcgcccg acataaagaa
     2881 acccgttacc tgatcgcggc cgtcctgact ggcgacggtt gttttgaaga agcccgaacg
     2941 gatggcaaag agcgaggtaa aggcttcgcc gacacagaac aggtattcgc cctttttcag
     3001 gcggcggctt tgacggatga cggcatcgag ttggctgagc tcgttgggca gcagcccgac
     3061 aggcaggcag agttcccgca aagaacagga agaacacagc gttttcatct gatgtgtagt
     3121 attatgcgaa gccataccgt acctttttgt gcgctttgcc ccatcatgat tatagtggat
     3181 taaatttaaa ccagtacggc gttgcctcgc cttgtcctta tttaaattta atccactata
     3241 tgtgcttatt gacacatatc aagacaggtt tatcatactg tggcattcta ccaaactatc
     3301 caaaatttga ctaacatcat aaaaccgcac acacacacca tgaaaatcat tcagatacag
     3361 aacaatcaca atgtaaacga tgaccgcccc gagtttgacc gcgcgctgat tgccagcctg
     3421 cccgccagcg gcccgcgcta cacttcctac cctaccgccg accgtttcca tgacggtttc
     3481 cgcgaaggcg aatatatcaa agctttacat ttgcgcggta tgggcgcgtt aaacaaaccg
     3541 ctttcccttt acattcacat tccgttctgc aacaccatct gctactactg cggctgcaac
     3601 aaaatcatca ccaaagacaa aagccgcgcc gatgcctaca tcgaatatct tgaaaaagaa
     3661 atggaactgc tcgctccaca tctgaacgga cggcaccagc ttgcccaact gcacttcggc
     3721 ggcggcacgc cgaccttttt gagcgacgaa cagatcgaac gtgtcttccg catgatacgc
     3781 aaacatttcg agttaatccc caccggcgaa tactccatcg aaatcgaccc gcgcaaagtc
     3841 agccgcgaca ccgtcctcat gctcggcaga ctcggcttca accgcatgag catcggcatt
     3901 caggatttcg accccaaagt gcaggcggcg gtcaaccgca tccaaagtta cgaagaaacc
     3961 aaagaagtca tcgatgcggc gcgcgaagcg gggttcaaat ccgtcagcgt cgatttgatt
     4021 tacggcctgc cgcaccagac ttcggaaagc atcaaaacca ccatcgatac cgttttgtcg
     4081 ctcgatcccg accgcctcgc cctttatcac tacgcccacc tgccgcacgt gttcaaaccg
     4141 caacgccgca tcgataccgc cgccgttccc gacagcgaag agaagctcga tatgctgcaa
     4201 tactgcgtcc aaaccctaac cgaacgcggc tacgtcttca tcggcatgga tcatttcgcc
     4261 aaacctgacg acgaactctc catcgccctc aaagaaggct tcctccagcg caacttccaa
     4321 ggctattcga cctacgcgga ttgcgatttg gtcgccatcg gcgtgtcgtc catcggcaaa
     4381 atcggcagca cctattccca aaacgaacgc gacatcgatg cctactatgc cgccatcgac
     4441 gaaggcagac tgcccatcat gcgcggctac cagctcaatc aggacgacat cctgcgccgc
     4501 aacatcattc aggatttgat gtgccgtttc gcgctcgact atcggattta cgaaagtatg
     4561 ttcggcatcc cgttcgaccg ctacttcaaa gacgaactgg cggatttgga aaaactcgcc
     4621 ggtttgggat tggtgcgcct gaacagccac gggctgaccg ttaccccgaa aggacgcttc
     4681 ctcatccgca acatcgccat ggtcttcgat taccacctgc gccataaaga aaccaaggcg
     4741 aaatattcgc aaacagtgtg attgtggcta acgtacaaat gccgtctgaa aggctttttc
     4801 agacggcatt ttgctgccgg caggataagt gttttcaaga acaggcggcg gcatatcata
     4861 acgttccgca cctttgtgtc cgaccgttcc gaaaccaaga tatagtggat taacaaaaac
     4921 cagtacagcg ttgcctcgcc ttgccgtact agctgtactg tctgcggctt cgtcgccttg
     4981 tcctgatttt tgttaatcac tataccaaac ggcatatccc gacagaacag attgtgcata
     5041 aggcacaagc ccgcacattc catcaacaaa atgccgtctg aacacgggtt cagacggcat
     5101 cagtatttta caatcagaat actgcctgta aaaccaagta gcagacaacc gacaatacgg
     5161 cggcggcagg cagggtaatg acccacgcca aaccgatggg cttcatcagt ttccagttgg
     5221 cattgcggtt gaccagaccg ataccgagta ccgcgccgac caagatatgc gtactggaca
     5281 cgggcagccc catcagcgac gcgcccatca cgacggaggc ggcggacagt tcggcggtaa
     5341 aacccgaagc aggatgcatt tccgccaaac tcgtaccgac ggttttaatc acctctttac
     5401 cgacaaacca caaaccgaca atcagcgcga tgccgaaagt cagcatcgca atcgggggga
     5461 cgacattttg cgcggcaacg ctgttggtac gcaaaacatc cataatcgcg gcaaacggac
     5521 cgatggcgtt ggcgatatcg ttcgcaccgt ggctgaatgc gaagccgcag gcggtaaaga
     5581 cctgcatcca tgaaaacatc tgaaaggtcg atttgcccaa gtctttacgc ttgaggcttt
     5641 tggcaaaaac aaacgtcccc atccacaccg ccgcgcctat cataaagatg gtcaggaagc
     5701 tgttgacgtt gctcatcccc aaatgcaggt ttttcaagcc cttgaaaatc agcatagcgg
     5761 aaatcatcat cgcgccgaac gaagcgataa agggaatcca agaatgcagt gccttgtagg
     5821 aatcgacatt gtttttacgg ttgtcgaacg cataaagacc gcggtaatac tccgattgca
     5881 gctcttgcgg atcgaattcg ggttcgtcgt aaatttgcgc gtcgtgcgcc attttggtgg
     5941 cgtactcgac tttttcggct tcggacaaac cctcgaaaaa caggcggtgc cgttctttat
     6001 aggccttttt ttcctgcttg atgcccttga gcgtgccttc cgcccaagcg ttgtaatcta
     6061 agacgttttt cttgacgcgc gaaaacagaa aataggacac cgcgccgccc aacacgggcg
     6121 acaataccca agaaacacca ataccgccca gcttgcccca acgtatcaaa tcgcccgatg
     6181 cggcatcgtt cattaccgcc atacataccg cgctgccgac aatgccgccg ataatggaat
     6241 gggtggtaga taccggaagc ccttttttcg aggcaaacaa cagccacaac gccgccgcca
     6301 aaagcgcgga catcataata aacacaaact gtatgggttc gaaatcaaca cccttcaaat
     6361 cgacgatgcc tttgcgtatg gtattggtta cctcgccgcc cgcgatgacc gcgccgctga
     6421 cctcaaatac cgccgcaatc agcaaagcct gcgggatggt cagcgtaccc gcaccgacgc
     6481 tggtgccgaa agaattggca acatcgttgc cgccgatgtt gaacgccata aacacgccga
     6541 acatcgtggc aataatgaat aaggcggcgt aattgtaatg ggtataaccc aagccccaat
     6601 aaataaaata gccgactgta cctaccagca tggcggcaaa gacggcgttg atggttttaa
     6661 catttgcgct catttggatt tgagccatgg caatcttcct ttaatgtggt ggaaatcatg
     6721 agtgttgccg acactgtact gctgcctccg gtcgaactgc cgtttcggga tatggcagac
     6781 gcatccttcc taaccgcatc cgaatacccc caaacctgac cgaatgcaaa actgctgccg
     6841 gaaacggttt gtatgacact tctactactg gatgttgcgg gcgcattata atatttttcc
     6901 atccgtcttg aaacatttat ttacacttta tttacactgc ggcggcaaat cggtatacga
     6961 gcgtcaatac acgttaaaat ggcgttttgc accagtttgg gagtgatgat ggaaacacag
     7021 ctttacatcg gcatcatgtc gggaaccagc atggacgggg cggatgccgt actgatacgg
     7081 atggacggcg gcaaatggct gggcgcggaa gggcacgcct ttacccccta ccccggcagg
     7141 ttacgccgcc aattgctgga tttgcaggac acaggcgcag acgaactgca ccgcagcagg
     7201 attttgtcgc aagaactcag ccgcctatat gcgcaaaccg ccgccgaact gctgtgcagt
     7261 caaaacctcg caccgtccga cattaccgcc ctcggctgcc acgggcaaac cgtccgacac
     7321 gcgccggaac acggttacag catacagctt gccgatttgc cgctgctggc ggaacggacg
     7381 cggattttta ccgtcggcga cttccgcagc cgcgaccttg cggccggcgg acaaggcgcg
     7441 ccactcgtcc ccgcctttca cgaagccctg ttccgcgaca acagggaaac acgcgcggta
     7501 ctgaacatcg gcgggattgc caacatcagc gtactccccc ccgacgcacc cgccttcggc
     7561 ttcgacacag ggccgggcaa tatgctgatg gacgcgtgga cgcaggcaca ctggcagctt
     7621 ccttacgaca aaaacggtgc aaaggcggca caaggcaaca tattgccgca actgctcgac
     7681 aggctgctcg cccacccgta tttcgcacaa ccccacccta aaagcacggg gcgcgaactg
     7741 tttgccctaa attggctcga aacctacctt gacggcggcg aaaaccgata cgacgtattg
     7801 cggacgcttt cccgttttac cgcgcaaacc gtttgcgacg ccgtctcaca cgcagcggca
     7861 gatgcccgtc aaatgtacat ttgcggcggc ggcatccgca atcctgtttt aatggcggat
     7921 ttggcagaat gtttcggcac acgcgtttcc ctgcacagca ccgccgacct gaacctcgat
     7981 ccgcaatggg tggaagccgc cgcatttgcg tggttggcgg cgtgttggat taatcgcatt
     8041 cccggtagtc cgcacaaagc aaccggcgca tccaaaccgt gtattttggg cgcgggatat
     8101 tattattgag cg
//
LOCUS       DAWISC010000087         8105 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWISC010000087 DAWISC010000000
VERSION     DAWISC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.087"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(300..686)
                     /locus_tag="WIW87_001321"
     CDS             complement(300..686)
                     /locus_tag="WIW87_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ5174478.1"
                     /translation="MPSTCRDAASAVCTKTPEYYPNHGEWPKNNTSAGVASSSTIKGK
                     YVKEVTVANGVITATMLSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRTDAKADT
                     VAAAANGKQIDTKHLPSTCRDASDAS"
     gene            complement(1005..1332)
                     /locus_tag="WIW87_001322"
                     /pseudo
     CDS             complement(1005..1332)
                     /locus_tag="WIW87_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1361..1690)
                     /locus_tag="WIW87_001323"
     CDS             complement(1361..1690)
                     /locus_tag="WIW87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5174479.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1768..1986)
                     /locus_tag="WIW87_001324"
     CDS             complement(1768..1986)
                     /locus_tag="WIW87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ5174480.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            2108..3061
                     /locus_tag="WIW87_001325"
     CDS             2108..3061
                     /locus_tag="WIW87_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ5174481.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALRRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3136..5193)
                     /gene="metG"
                     /locus_tag="WIW87_001326"
     CDS             complement(3136..5193)
                     /gene="metG"
                     /locus_tag="WIW87_001326"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ5174482.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHACH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGTKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSELLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRDTLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(5309..7147)
                     /gene="glmS"
                     /locus_tag="WIW87_001327"
     CDS             complement(5309..7147)
                     /gene="glmS"
                     /locus_tag="WIW87_001327"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ5174483.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGLYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPSDVEIASEYR
                     YRSVIADSDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            7429..7950
                     /locus_tag="WIW87_001328"
     CDS             7429..7950
                     /locus_tag="WIW87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174484.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKIHVVTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQHLAAIEQLKRRFEAEFDELEK
                     EIKCNGRSPALLL"
BASE COUNT         1686 a         1932 c         2413 g         2074 t
ORIGIN      
        1 acgggatgca ggtttccgta tgaatggatt cgtcattccc gagtaggcgg gaatctagtc
       61 cgcttgttcg gtaaatgaga gggcggattg cgcgcctgtc agataaacca cgtgtttaaa
      121 cgggcggcaa tgaggtacgc gcagagcctt gaagcgcaat cgatatatta ttttcagcca
      181 aaacggacgc ccccgcttgc cttgcaaacc tttaaaaagg aagccacccg gattaatccg
      241 agtggccgtg gaaaatcact taccgcttga tttatttaaa atttatggta taatttacct
      301 tagctggcat cacttgcgtc gcggcaggtt gacggcaggt gcttggtgtc gatctgcttg
      361 ccgttggcgg cggcggcgac ggtgtcggct ttggcgtcgg tgcgcgtaac cggctgtccg
      421 cagaaccatt ttaccgaacc gtcttgacgc ttggcccaca gggagagttt tttgccttgg
      481 atttctttgt ttacgccgct tgaaagcatt gtggcggtaa tgacgccgtt tgcgactgta
      541 acttccttaa catatttgcc tttgattgtt gaagaagatg ccacgccggc agaagtgttg
      601 tttttcggcc attcgccgtg attcgggtaa tactcgggtg tttttgtgca aacggcagat
      661 gctgcgtcgc ggcaggttga cggcaggtgc ttggtgttgg ttttcttgtt gccggtgttg
      721 tcggcggcga cggtgtcgtc ggtgccggcg cgcgtaaccg gctgtccgca gaaccatttt
      781 accgaaccgt tttgacgctt ggcccacagg gagagttttt tgccttggat ttctttgttt
      841 acgccgcttg aaagcattgt ggcggtaacg acgccgtttg cgactgtaac ttccttaaca
      901 tattttcctt tgattttaga ggaggatgcc acgccggcgg catcattaaa tcccgtcatt
      961 cccacgaaag tgggaatcta gaactcagga ccggagaaac ctttttaccc gataagtttc
     1021 cgtgccgaca gacctggatt cccgcctgcg cgggaatgac gaagtttttc ggccattcgc
     1081 cgtgattcgg gcaatactcg ggtgttttgt gcaaacggca gatgctgcgt cgcggcaggt
     1141 tgacggcagg tgcttggtgt caatcttctt accgttggcg gcggcggcgg cggtaacgtc
     1201 gtcgttggcg gctttggcgt tgtcgcgctc aaccggctgt ccgcagaacc attttaccga
     1261 accggcttga cgcttggccc acagggagag ttttctgcct ttgatttctt tgtttacgcc
     1321 gcttgaagcc attatgtcag acggtattgc ccgggcagct ttattcgtac actttcagca
     1381 gctcgacttc aaatatcaaa gtggcgtgcg ggggaatcac gccgcccgcg ccgtgtgcgc
     1441 cgtagcccat ttccgaaggg atggtcagct tgcgtttgcc gccttccttc atgccgccga
     1501 agccttcgtc ccagcctttg atgacttgtc cgacaccgag cgtgatggtc agcggctggc
     1561 ggcggtcgag gctggagtcg aatttggttc cgttttccag ccaacctgtg taatgcacgg
     1621 taatctcttt gcctttaact gcttcttttc cgaagccttc ttgcaagtct tcaataatca
     1681 ggccgcccat atttgtcctt tcgttgcttg ttggtcaaaa cggcaagggt aacataccgt
     1741 ccgtcgaagt caaatgccgc tcaaacgtca gctgcatcgg tgcagctgaa acggctgtgt
     1801 ttgtttgact gttttatttt tttcgtaaag gttccatgct ttttcatgga aatagaaaac
     1861 gacggtgttg attaggggtt cgaccagcgc aactgctccc gatacgccta tactgcccgt
     1921 cagtacatag gttacactga aggcgacgct gaaatgcagt gcggcaaaag tcagggtttt
     1981 aagcatcatc ctctcccgga ttggacattg acggagagat gataaagatt atcataaggc
     2041 tgcgcggttt aaatttgcta tttgttgtta gtgtagataa atcgtttttt aaataaggat
     2101 aggaattatg aatcataaaa agatcgttgt tttggatgcg gatactttgc ccggccgggt
     2161 ttttcatttt gattttccgc acgagcttgc ggtttacggt acgacaggtg cggatgaaac
     2221 ggcagaacgg gtgcgcgatg cacatattgt cattactaac aaagtgatga tttctgccga
     2281 tattattgcg gctaatccgc agttggagct gattgccgtc agtgcgaccg gcgtgaacaa
     2341 tgtcgatatt ggggcggcga aggcggccgg tgttgcggta tgcaatgtcc gcgcatacgg
     2401 aaacgaatcg gttgcggaac acgcctttat gctgatgatt gcgttaaggc ggaatttgcc
     2461 tgcctatcag cgtgatgttg cggcaggatt gtgggaaaag tcgccgtttt tctgccatta
     2521 cggcgcgccg attcgggatt tgaacggcaa aacgctggcg gttttcggac gcggcaatat
     2581 cggacggacg cttgccggat acgcgcaggc attcggtatg ggggtggtgt ttgccgaaca
     2641 caaacacgcg tccgctgtgc gtgaaggcta tgtttccttt gaagatgcgg tacgggctgc
     2701 tgatgtgttg tcgctgcact gtccgctaaa cgcccaaact gaaaatatga taggcgaaaa
     2761 cgaattgcgg cagatgaagc ctggcgcggt tttaatcaat tgtgggcgcg gcgggctggt
     2821 ggatgaaaac gcgctgcttg ccgcactcaa atacgggcag atcggtgggg caggtgtcga
     2881 tgttttgacg aatgagccgc ccaaaaacgg caatcccttg ctgaatgcac gattacccaa
     2941 tctgattgtt acgccgcata ccgcgtgggc aagtcgtgag gctttggaca ggctgtttga
     3001 tatattgttg gcgaacattc acgcctttgt gaaaggagag gcgcaaaacc gcgtggtttg
     3061 aacctgtcgg gattgcggaa aaaaatgccg tctgaacgcc tcaagggttc agacggcatt
     3121 tcttgagatt cccgtttaac cgactttgtc gcccggctgc gcgcctgtat ccacatccaa
     3181 gagcttcagt ttcccgtctg ccgtggcggc actcaaaatc atgccttcag atacaccgaa
     3241 ttttgccatt ttgcgcgggg cgaagttggc gacggcgatg accatgcggc cgttcaattc
     3301 ggcagggttc gggtaagacg cggcgatgcc ggagaagatg atgcgttttt caaaaccgaa
     3361 atcgaggtcg aatttcaaaa gtttggtgct gccttcgaca gcttcgcagt tcaatacttt
     3421 ggcaacgcgc atgtcgattt tcataaagtc gtcgaaactc gcctgttcgg cgactttttc
     3481 gtatttgccc tcttcggcgg caggtgcggc tgcggcggcg atgctttgtt tgttggcttc
     3541 gattaaatcg tccacttgtt tttgctccac tcgttgcatt aaatgttcgt atttgttgat
     3601 ggcgtgtttg cccaaggtat cgcgtgtatt tgcccaagtg atggcttcca aattcaggaa
     3661 tttggcggcg tttgcggcgg tttgcggcaa gacgggggcg aggtaggcgg tcaacatggt
     3721 gaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt tcgtcttggc cttcttgttt
     3781 ggcgagttcc cacggcttgt tggcatcaac gtattcgttg acaatgtctg ccaaggccat
     3841 gatgtcgcgc agggctttgg cgtattcgcg gctttcgtag cattcggcaa tggcttcgct
     3901 ttgcgcagtc agttttgcca gcaattcgct gtcggcaaca tctttcagac ggccttcaaa
     3961 gcgtttggcg atgaaacctg aggcgcgggc ggcgatgttg acgtatttgc cgacgaggtc
     4021 gctgtttacg cggctgataa agtcttgcag gttcaaatcg atgtcttcga ttttgctgtt
     4081 gagtttggcg gcgatgtagt agcgcatcca ctcggggttc aggccttgtt ccagatagga
     4141 tttggcggta ataaacgtgc cgcgcgattt ggacattttt tgtccgtcga cggtcaaaaa
     4201 gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag aaatgcagca tagcgggcca
     4261 gaacagggcg tggaaataga gaatatcttt gccgatgaag tggtacatct cggtttggct
     4321 gtcggctttg aagtattcgt caaaatcgac gccgatgcgg tcgcacaggt ttttaaacga
     4381 cgccatgtag ccgacgggcg cgtccagcca gacgtagaag tatttgcccg gcgcgtcggg
     4441 gatttcaaaa ccgaaatacg gcgcgtcgcg ggaaatatcc cagtcggaca gggtggtttc
     4501 ttcaccttcg cccagccatt ctttcatttt gttgagggct tcggcttgca gatggggctt
     4561 gccgtcgtgc gggttgttgc cggaagtcca tgctttgagg aagtcggcgc attcgcccag
     4621 tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttc gtaccggaaa cggcggaata
     4681 cgggttaatc agttcggtcg gggaataggt cgtgccgcag acttcgcagt tgtcgccgta
     4741 ttggtcttgg gcgtggcatt tcgggcattc gcctttgacg aagcggtcgg gcaggaacat
     4801 ttgtttttcg gggtcgaaaa gctgctcgat gacgcggctc tcaatcttgc cgttggcttt
     4861 cagcgcgcgg taaatgtctt gggaaaactg tttgttttca ggggaatggg tgctgtaata
     4921 attgtcgtaa ccgatgaaaa agccagtaaa gtcggcgagg tgctcttcgc gcactttggc
     4981 aatcatgtct tcgggcgcga taccttgttt ttgcgcggca agcattacgg gcgtgccgtg
     5041 ggtgtcgtcg gcgcagcagt agtggcacgc gtggccgcgc agtttttgaa agcgcaccca
     5101 aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg atgctgccgt tggcataggg
     5161 cagggcggag gtaactaaga ttttgcgtgt catattgtgc tttgcaaaca atgggtaaag
     5221 gcggattata ccgcaaatca aacggggaaa tgccgtctga agcctgaaaa atcgggcttc
     5281 agacggcatt tttgccaacc ggcgggagtt attcgacggt tacggatttc gccaggttgc
     5341 gcggcttgtc cacatcggta ccgcgtgcga gggcggtgtg gtaggcgagg agctgcacgg
     5401 ggatagtatg cacgacgggg gacagtttgc cgacgtggcg cggtgcgcgg ataacgtgca
     5461 caccttcggt ggcattaaaa ttgctgtcga ggtcggcaaa gacgaaaagt tcgccgccgc
     5521 gcgcgccgac ttcctgcata ttggctttga ctttgtccaa caggctgtcg ttgggtgcga
     5581 tgacgacgac gggcatattt tcgtccacca gggcaagcgg cccgtgcttc agttcgccgg
     5641 caggataggc ttcggcgtgg atgtaggtga tttccttcag cttcaacgca ccttcgaggg
     5701 caatcgggta atggatgccg cgccctaaaa acagcgcgct ggttttcttg gcaaactgtt
     5761 gcgcccatgc ggcaatttga ggttcgaggt tcagagcgtg ctgcacgctg ccgggaagct
     5821 ggcggagttc ttcggtgtaa cgcgcttcgt cttcttcgga aaccaaaccg cgcactttcg
     5881 ccagcgttac cgccaaaccg aacagcgcaa ccagttgcgt ggtaaacgct ttggtcgagg
     5941 cgacgccgat ttccgcaccg gcacgggtat aaagcacgag gctgctttcg cgcggcaggg
     6001 cggattccat cacgttgcaa atggagaggc tgtggcggtg tcccaaggat ttggcgtatt
     6061 tcaacgcctc catcgtgtcc agcgtttcgc cggattggga aatggtaatg accagttggt
     6121 cggaatcagc aatcacgctg cggtatcggt attcgctggc gatttcgacg tcggacggga
     6181 tttttgcgat ggattccaac caatatttgg cggtcagcgc ggcgtaatag gacgtgccgc
     6241 aggcaaggat tttgacgctg cggatgcttt caaacacgct tttggcatct ttgccgaagt
     6301 tttcggggat gaagccgccg tcgaggaaaa cctccgccgt gtctgcaatc gcgcggggct
     6361 gctcgtggat ttctttttgc ataaagtggc tgtacagtcc cagttccaaa gaggcgagcg
     6421 agagttcgga taccttgact ttgcgttcgg caggcaggcc gtttttatcg gtcagccttt
     6481 tgatgccgtc tgaagccagc agcgcgatgt cgccgtcttc gaggtacgcc acgcggcgcg
     6541 taaaggcgat gacggcggat acgtccgaag cgataaaggt ttcatcgtcg cccaaagcga
     6601 ccaaaagcgg gcagcccata cgcgccacaa ctaattcatc aggcttgtct tgggcaataa
     6661 ccgcgatggc gtatgcgccg tggaaacgtt tgaccgcttc ttgtaccgct tcaaacagcc
     6721 tgccgccgtt ttgcgcgtat tcgtgattga tgctgtgtgc gatgacttcg gtatccgttt
     6781 gcgattcaaa acggtatccc aaaccttcca aacgtttgcg ttcgctttca aagttttcga
     6841 tgatgccgtt gtgtacgacc gcaatcatac cgccgctgat gtgcgggtgg gcgttcggct
     6901 cagtaacgcc gccgtgtgtc gcccaacgcg tatgtccgat gccgatgccg ccgctgatgc
     6961 ctttttcgcg tgccgcgtcc tccataagct gcacgcgtcc gacgcggcgc acacgtttga
     7021 ttttgccgtc ggtgttgacg gcaatgcctg atgagtcata accccggtat tcgaggcgtt
     7081 tgagaccgtc ggtcagaaaa tcgacgacgt tgtgatgggc gcggatggcg ccgacgatac
     7141 cgcacataac tgttccttag tatccggttg aaaaaaaaca ggcgcggacg gcttccgtgc
     7201 cgcaccttcc tcttcggatt ataaaccgcc tcccgcgccg gaaaacagca aaatgccgtc
     7261 tgaaggcttg ggcttgctca aaaaaaggag ggatttccct gtttatccag gatgggcgtt
     7321 cagacggcat tacctgctgc tggtttatag tttttgcaaa tcaacattga caagctgaaa
     7381 aaaaaaaaca atatactcgc tcggtcttaa tgttaacgga gtatggaaat gaaacaaatg
     7441 cttttagccg tcggcgtggt ggcggtgttg gcgggctgcg gcaaggatgc cggcggttac
     7501 gagggttatt ggcgcgaaaa gtcggacaaa aaagagggta tgattgccgt caaaaaagaa
     7561 aaaggcaatt acttccttaa taaaatccac gtggttacag gcaaggaaga gtccttgctt
     7621 ttgtctgaaa aagacggcgc gctttcgata aacacaggga taggggaaat cccgatcaaa
     7681 ctttccgacg acgggaaaga gctgtatgtc gaacgtaggc agtatgtcaa aaccgatgcg
     7741 gcgatgaagg acaaaatcat cgcccatcag aaaaagtgcg gacaaacagc acaggcatac
     7801 cgcgacgcgc gaaatgcgtt gccgtcaaac cagacgtatc agcagcatct ggcggcgatc
     7861 gagcaattga aacggcggtt tgaagccgag tttgacgaat tggaaaaaga aatcaaatgc
     7921 aacggcagaa gcccggcatt gttgctttag taggggacaa ccgggaggat gccgccgtcc
     7981 gaatcggatg tgcggtttct gtaccggtac gggcgggcag gaatgtccgc cttttttgtt
     8041 cggatgcgtt tgaatacccg tttgattccg accgtttgca aggggtattt ccgttcgggc
     8101 ggaaa
//
LOCUS       DAWISC010000088         8020 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWISC010000088 DAWISC010000000
VERSION     DAWISC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.088"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(68..814)
                     /locus_tag="WIW87_001329"
     CDS             complement(68..814)
                     /locus_tag="WIW87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ5174485.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WIW87_001330"
     CDS             complement(993..1664)
                     /locus_tag="WIW87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ5174486.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIW87_001331"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIW87_001331"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ5174487.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WIW87_001332"
     CDS             complement(2481..2963)
                     /locus_tag="WIW87_001332"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ5174488.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WIW87_001333"
     CDS             complement(2995..3684)
                     /locus_tag="WIW87_001333"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ5174489.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIW87_001334"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIW87_001334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ5174490.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WIW87_001335"
     CDS             complement(4571..5881)
                     /locus_tag="WIW87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5174491.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIW87_001336"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIW87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ5174492.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WIW87_001337"
     CDS             complement(6056..6355)
                     /locus_tag="WIW87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174493.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WIW87_001338"
     CDS             6706..7902
                     /locus_tag="WIW87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ5174494.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1891 a         2404 c         1962 g         1763 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgttgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt cggacgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccaccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaacccc gatacttcgt tcaaagaaac gacgggaata tcgtactggg
     2221 caagcagttc gccggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atatattcat
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatgcgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatcc gcccgagata gccgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat gtgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaagcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacagag caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcctgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac
//
LOCUS       DAWISC010000089         7957 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWISC010000089 DAWISC010000000
VERSION     DAWISC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.089"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            66..260
                     /locus_tag="WIW87_001339"
     CDS             66..260
                     /locus_tag="WIW87_001339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174495.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPSRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WIW87_001340"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WIW87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ5174496.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1091..1480
                     /locus_tag="WIW87_001341"
     CDS             1091..1480
                     /locus_tag="WIW87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ5174497.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WIW87_001342"
     CDS             1558..2388
                     /locus_tag="WIW87_001342"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ5174498.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WIW87_001343"
     CDS             2403..3860
                     /locus_tag="WIW87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ5174499.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMVFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WIW87_001344"
     CDS             complement(3901..4290)
                     /locus_tag="WIW87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174500.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDIPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4472..4873)
                     /locus_tag="WIW87_001345"
     CDS             complement(4472..4873)
                     /locus_tag="WIW87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174501.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WIW87_001346"
     CDS             complement(5290..5619)
                     /locus_tag="WIW87_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174502.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..6352)
                     /locus_tag="WIW87_001347"
     CDS             complement(5645..6352)
                     /locus_tag="WIW87_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ5174503.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6487..6804)
                     /locus_tag="WIW87_001348"
     CDS             complement(6487..6804)
                     /locus_tag="WIW87_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174504.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(6807..>7957)
                     /gene="mafB"
                     /locus_tag="WIW87_001349"
     CDS             complement(6807..>7957)
                     /gene="mafB"
                     /locus_tag="WIW87_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ5174505.1"
                     /translation="QRQHYEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQ
                     QANINGTIGYHTRFSGHGHEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWEGHEHHP
                     ADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNF
                     SDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAE
                     TVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNV
                     DFKAKSIGTKIHDGAQGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGA
                     RGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         1896 a         2169 c         1737 g         2155 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgtcgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cagcaaagca
      301 gccggatacc gctacgaatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccaccggcct cggcgtattg gcgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgccggtgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtga tggcggaatt
     1381 tattgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctccctgc caaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg cgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcatcgc caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gcttcaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggcggcg atgccgatgt acctgtattt cccgaatatg gtctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggct accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atattgtctt
     3901 ttaaaaaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctatactc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg aatatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt ttccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggtattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct
     4561 tttcaaaaaa atcaaaactt tcttctttca accagtccat ataactatca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catccgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tccacataaa atctgtgatt attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccggat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat gaaagaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaaggaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcag aaccgttatt aaaagtttga gttttgttta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgact gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggctt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgcgccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     6121 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     6181 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ttgatacgga ttgccgtatc
     6241 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     6301 cgccggttcg gatacgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     6361 cgtggaagga gcaggtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat
     6421 gaaggaaatg ccgtctgaaa attaaacacg gtttcagaag acattgtgat tgttggtttt
     6481 tttaacctac gcttgcgaaa ttaattcctt tctcctgaat actgcaccat ttatttggtt
     6541 taactccata gaagaatgat tagaataaat acattcaaag tcagcttcat caaaatattc
     6601 tccacacata gaccataact ctgtaatcaa atattccata tcttcatggt cttctgtgat
     6661 ggcttctctt gataaataat ttttgatttt taaaaacctt cttccttcct cgtaatctac
     6721 ggtaattaac ctgatatctg agtgtatcat gcccagcatt gctatattca taccgactcg
     6781 cagccagctt ggtaatatat tcataactat tgcacctttt taatggtttt agggttagcc
     6841 ggaacaatgt gaactccatt tttacctgaa tgaatcgtac caaaattggt acttaatcct
     6901 gatttcccat cgctgcctat aggataccca aaatcaacaa caggatttcc tcttgctcct
     6961 ttagaaataa ctggataagc acctgaatgt attccgttca acaattcttg aggattaatg
     7021 ttttgattta aagtactctt accttcaatg tagtttctat gtcctgaaat atgtttccct
     7081 tgagctccat catgaatttt agtcccaata gattttgctt taaaatcaac attgcgggtt
     7141 tgaaacattt ctccatctac agactgagat aatcttgaag cagtgttatg cttgtaggag
     7201 tctgagaaat ccccactaac cgcagccttc cccggttttg ccgcctttgt caggtttttg
     7261 actttggcaa acggcaggac gttgaccagg gcttcgacgg tttcggcggc attggggttt
     7321 tcctgcatcc actggtcaac ggcttcgccc gcgctgataa aggggttgag cgcgccggcg
     7381 gcgacgccgt tgataaactc catgctgttg ccccagcggt cgagcttggc attgtgctcg
     7441 aacatttttc tgttggcttc atcggcgcgg tcggagaaat tgctgccgag gttgttgtag
     7501 ttgtcggata tgcgttgccg gatgctgcgg gtgtcggtcg gattgagttt gatactgcgg
     7561 gctgtgccgt tgacgtgata ggtgtattcg tctcgtgcgc ccgtaggttt ggggtaattg
     7621 ccgcccttcg ggccgtcgta ggcatcggcg ggatgatgtt cgtgtccttc ccagttgagc
     7681 cggtatacgg taaagccttc gtcaacgttg cctttttctt cgctcgcgct gtcggcggcg
     7741 tggttgtcga agggggcgtg ttcttcgtgt ccgtgtccgg aaaagcgggt gtggtagccg
     7801 attgtgccgt tgatgtttgc ctgttggatg agcaggttgc ccatctggtg ggtatagtct
     7861 tggatgacgt tgattttgcc ggtgcggtcg gaaacgctgc cgcgcgggtc gccgaagagg
     7921 tggtatttgc cgccgggttc gtagtgctgc cgttggg
//
LOCUS       DAWISC010000090         7906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWISC010000090 DAWISC010000000
VERSION     DAWISC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.090"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(51..2093)
                     /gene="recG"
                     /locus_tag="WIW87_001350"
     CDS             complement(51..2093)
                     /gene="recG"
                     /locus_tag="WIW87_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ5174506.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WIW87_001351"
     CDS             complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WIW87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ5174507.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WIW87_001352"
     CDS             complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WIW87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ5174508.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3113..4606
                     /gene="rng"
                     /locus_tag="WIW87_001353"
     CDS             3113..4606
                     /gene="rng"
                     /locus_tag="WIW87_001353"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ5174509.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4641..5891)
                     /locus_tag="WIW87_001354"
     CDS             complement(4641..5891)
                     /locus_tag="WIW87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ5174510.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADDDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALPLQAER
                     LNNMPLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDEIPLTKTTFGLQSRFRQV
                     LQELMPLALEKCQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGKGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELSDSRRFGRGLLIRALLDKETLK"
     gene            complement(5887..6062)
                     /locus_tag="WIW87_001355"
                     /pseudo
     CDS             complement(5887..6062)
                     /locus_tag="WIW87_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            6136..6366
                     /locus_tag="WIW87_001356"
     CDS             6136..6366
                     /locus_tag="WIW87_001356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174511.1"
                     /translation="MPVFFKTAAQKNRAGNCLQTALGRDCNTRAGRQSLRQTTGTIQA
                     SDGFAVYGRGTIPAAIELANIASDKPHAQKNE"
     gene            complement(6285..7598)
                     /locus_tag="WIW87_001357"
     CDS             complement(6285..7598)
                     /locus_tag="WIW87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ5174512.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1858 a         2341 c         2058 g         1649 t
ORIGIN      
        1 aaatcagatt tccgttacac ttttttccaa tatttcagac ggcattttgc tcacacgccc
       61 aaataccctt ccctgccgga aagccacctt gccaaatgcg cttcgacgat ttcggggttt
      121 tgttcaatca gcatcggggc ggtttcgcgc gcttgttcca agaggtgcag gtcttcttcg
      181 agcttggcga aacgcagcat aggcacgccg ctttggcgcg cgccgagaaa ttcgccgggg
      241 ccgcggatgt tgaggtcttg gcgggcgatt tcaaagccgt cggtgtgttc gtagatgact
      301 ttcagccgcg ctttggcgag ttcgcccaag ggttcggcaa acaggaggac gcacacgctt
      361 tctgccgcgc cgcgcccgac ccgaccgcgt aattggtgca gctgcgccaa gcccatgcgc
      421 tcggcgtgtt cgatgaccat cagggcggca ttgggcacat ctacgccgac ttcgatgacg
      481 gtggtggcga ccaagacgtt cagccccccc gaagaaaacc gcgccatcac ttcggccttt
      541 tcggcggcct tcatgcgccc gtgtaccagt ccgatattga gttcgggcaa tgccgtctga
      601 agccgggcga gggtttcggc ggcggtttgc agttgcaggg tttcgctttc ttcaatcaat
      661 gggcagaccc aatacgcctg ccgccctttt cggcaagtgc cgaggacgaa gccttcgact
      721 tcggcgcggc ggacgttgtt gacgaggcgc gttttaatcg gtgtgcgccc gggcggcaat
      781 tcgtcgatga cggacacgtc caaatcggcg aaaaaactca tcgcaagcgt gcgcgggatg
      841 ggcgtggcgg acatcatcag ctgatggact tcgcgccctt tgtttttgag ggcgaggcgt
      901 tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca agcccaaatt gtgaaacgcc
      961 acgccgtctg aaaacagggc gtgcgtgccg acggcgattt tgacgctgcc gtcggcgagt
     1021 ttggctttgg cttcgtcttt ggctttttta cgcaaactgc caaaaaggcg gacaacttca
     1081 atgcccaaag gttcgagcca ttgtttaaat ttaataaaat gttgttcggc aaggatttca
     1141 gtgggcgcca ttacagccac ctgcgcaccg gattcgatag ccgtcaaagc agacaaagca
     1201 gccacaatgg ttttgccgct gccgacatcg ccctgcagca ggcggtgcat cgggtaggtt
     1261 tgcgccatat cgcggcagat ttcggaaaca actttttctt gcgcatcggt cagggcaaac
     1321 ggcagggctt ggcgcagggc ttgggtcaat gtgccgtcgc cgcccaatgc cgccgccgtg
     1381 ccgccgatac gcttctgtcg cgccaagcgc atcgaaagct gttgcgccaa aagttcatcg
     1441 aatttgagcc gttgccatgc aggcagcgtg ccgtctgaaa gctgatgaat cgtgaaactc
     1501 ggcggcggcg aatgcaaaag acgcaggctt tcggcgaggt gtggcagctt cagacggcac
     1561 agcagggcat cgggcagcgt gtcgtgcagc ggcgtaacgt ccaacgccgt ctgaataata
     1621 cggcgcaaag tgggctggtt caaaccgttt acggtcgggt aaaccggcgt gaggctttcc
     1681 gccaaaccgc cgccctcggc atcgcggatt ttgggatgaa tcatctcgtc gccgtaaaag
     1741 ccgtgtttga tttcgcccac ggcgcggatg cgtttgccga ccgccgtctg tttctgatgg
     1801 ctggcgtaaa agtggatgaa gcgcagaaaa aggacgctgc cggagccgtc ggcgatttgg
     1861 acaatcagct gcttgcgcgg tttgaacgtt acttcctgat ggataacctc cccctcgacc
     1921 tgacacggca cgccaatcgg cgcgtcctta atcggcataa tgtgcgtctc gtcctcgtaa
     1981 cgcagcggca ggtgcaacac caaatcccac gcggtatgga ggttgagttt gtcgagcttc
     2041 ttggcggaaa catcggtgat tttgagctgt tttcgggttt cgggcgacat cataggcaga
     2101 ttcctttgga cgcgcctatt ttatccgaaa acaaaaatgc cgtctgaaac ggattcagac
     2161 ggcatcgaca ggcaggaatc aagccccggc ggcttcggct tcctgctgtt gttggtaaat
     2221 cgcctcaaaa ttaatcggcg cgagcaggac gggcgggaag ccggcgcgcg ttaccgtacc
     2281 ggaaacggct tcgcgcgcgt aagggaagag gatgttcgga cacgccacgc cgagcagcag
     2341 gtcggcatct tcttcgggga tgttttccaa acggaaaata ccgctttggg ttacttcgtt
     2401 caaaaacatc gtgcgctcgt tatccaattt ggcggttacg gtaacggtta catccacgtt
     2461 gtagtagccg tcttccagct tttggctgcc ggtggaaacg cgcatctcca cttcgggctc
     2521 gccctgttcc aaaaagattt gcggcgcgtg cggcacttcc aaagacaagt ctttgacata
     2581 cagtcgctcg atgctgaata cgggttgcag ttcttcgctc attttgtttt cctagttggg
     2641 ggttaagggt tcagcagtcc gtccagcccg ccttcctgct ggaggcggta gaggtcggta
     2701 aatccgccga cgtgcgtttc gccgatgaaa atctgcggca cgctgcgctg tcccgaaagc
     2761 tgctgcattt cggcaaaggc ttcggggctt gcatcgacac ggatttcgtc gatatgtccg
     2821 acacctgccg cgtgcagcag ccttttcgcc atcgcgcagt aggggcaaaa cggacctgtg
     2881 tacatggtaa cggtctgcat attgggtttc cgaaagtttt gcaatgataa tcaatatagg
     2941 ggcatttccc ctgtttggca agtgcggaac agatgcacgt tcaaacggca tgtgcggaat
     3001 gtgtcaaagt ttctttttta aagtatgata gacattgtga aaaatatttt tgcacccgcg
     3061 ctgcgcggcg gaacggatgc aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     3121 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     3181 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     3241 gcgcaacagc gaacacagcc tagtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
     3301 gcctgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     3361 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     3421 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     3481 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     3541 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     3601 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     3661 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     3721 catccaagaa caggcgaaaa tccggccgcc cgaaaccctg ctttatcagg atttgccttt
     3781 aagcctgcgc gtgttgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     3841 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     3901 caggatagag ctgttcaaag gcgaacgccc gctgtttgaa acccacaacg tcgaacagga
     3961 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     4021 atccaccgaa gccatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     4081 cttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     4141 gaggctacgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cacaggaaag
     4201 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     4261 taccctgcac ggttttacca gcctagggct ggtcgagctg acgcgcaaac gctcgcgcga
     4321 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     4381 aacgccgcaa accgtatgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     4441 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     4501 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     4561 cgaaaccgct tacacgcagg aacaatacga catcgttttg atgtaaaaaa tgccgtctga
     4621 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaacgc gcgtatcagc
     4681 agaccgcgtc cgaaacgtcg gctgtcggac aattccaaat atccgccgta ttttttggca
     4741 agcgtgtcgg cgatggacag acccagcccc gtcccctgct gctccgttcc caaaatacgg
     4801 taaaacggat cgaggacacg ggcgcgttcg gattcgggaa tgcctttccc gttatcttcc
     4861 acccacacgg caagatattt cccttcgtcc gtgaaaccca aatctatcct gccttcgggc
     4921 ggcgtataac gtaccgcgtt gtcggcaaag gttttaatca gcgtatagat ttccgtttcg
     4981 tcggcagaca cttcgacatc gcctccgacc gccacgccga tgtcctgaca tttttccaaa
     5041 gccagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
     5101 ttcgtcagag ggatttcatc cgactgcgaa cgcgccaatg ccaaaagctg ttcgagcagg
     5161 tgtttgttac gccgtatgct ttgctgcaaa acggcaggct gccgcgccgc atcgggtggg
     5221 agcggcatat tgttgagccg ttccgcctga aggggaaggg cggtcatcgg cgtacgcaat
     5281 tcgtgtgccg cgtcggcgac aaaccgctga cggtggcgga tgtcttcatc cgcacgtttc
     5341 aaaagcaggt tgatggcggt tacgaaacct ctgatttcac tgggaatatt gtccacactc
     5401 aaagcagaca ggtcattgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
     5461 cgcatggctt tgtgcgtaat ccacacggtc agcaaaatca tcagcggcag tgccgccaac
     5521 aggggcaaca cgctttgccg tgccgcatcc gccgccaaat cttcacggta ttcgttttcc
     5581 tgcataacgg caatccgtcc ctgctcggtc gtgcggatat agacgcggta ataatcgtcg
     5641 tcatcgtccg cctgaagcgt gtgcagaccg tccgccagat gcgcaggcag gctgacaaca
     5701 gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat cgccgtcgcc ctcgggcaaa
     5761 gtttcgggtt tggaatcggg ggcgacgtac aatgccgcct gacggagcag gtcgtcctgc
     5821 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
     5881 ggaacaaaca tccaaaccgt cccgccccgg caaacccaaa tccagcagca tcaagtcata
     5941 aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata gccgccgtct
     6001 ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag caaaacacgc
     6061 atcaactttc ccttcaaaat aaaccgtgcc tattctaaca ccccaaaatt agccgcaatt
     6121 tagcggtctt tacgcttgcc ggtatttttc aaaactgcag cacaaaaaaa ccgcgccggc
     6181 aactgccttc agacggcatt ggggcgcgat tgcaacacac gggcaggcag gcagagcctg
     6241 cgacagacca caggaacgat tcaggcttca gacggcttcg ccgtttacgg cagaggcacg
     6301 attcctgccg ctatcgaact ggccaatatc gccagcgaca aaccccacgc ccagaaaaac
     6361 gaataacgga tgtgtttgcc catcgacaat ttcgccaaac ccaagcccat ccacaaagcc
     6421 ggcgaaagcg gcgtaacaaa agtgccgacg atactgccga tcaacatcgc ataacctgct
     6481 gcttcgggcg ccacgcccgc ctgcgaggta atctgctcca caatcggaaa cagtccgaaa
     6541 taataagcgt ccgtactcaa aaccaactca agcggaatgc ccaacacacc gatggcaata
     6601 tgcagataag gcagcagcgc gtccggcagg atatgcacaa tgtctttgga aatcgcgtcc
     6661 aacatccccg cacccttcaa aatccccaaa aacgtacctg ccgccaaaat aatggacgcc
     6721 atcatcaccg cgccgccggc gtgggcataa atccgctcca tctgttcctg cgggctgcgg
     6781 taattcaaaa gcaacgccgc cgttgcagcc agcataaata cataacccgg tgggaagatg
     6841 cccgaaaaaa gcaggctcat cgccgccaaa aacagcagga cattccacca aaacagtttc
     6901 ggacgcgcca atttttgttc ttcttccgac aaaggcaccg gctttatcaa atccgccacg
     6961 gcgggcaacg cgcccaactc ccggacaatc cgcctttttt cacgcacacc caaaagcagg
     7021 gacagcgcaa ggataaacac cacaccgata atttgcaccg tcaacaaagg tttatacaat
     7081 tcgcccacat ctgcgcccaa cacgcttgca acccgcccgg tcggcccgcc ccacggcaga
     7141 aggttaatca atcccgcact ggaagtcagc agcaaaaaca gcaggtaagg attcatatgc
     7201 agacgcttgt aaagcggcaa aagggcgggg acgaccaata aaaacgtcgt cgcacccgcc
     7261 ccgtccaact gcgccaccac cgacaccaag accgtcccca cactcactgc cacgatatta
     7321 ccccgagtca gcttaatcaa accgcctatc atcggacgga acagccccac atcgttcatg
     7381 attccaaaaa acaaaatgga aaacataaac ataatcacaa tctgcatcac cgatttggtg
     7441 ccgcccgaat aaaattcttt taattgggat acatcaaacc ccgccagcaa cgccccaaac
     7501 agcggcacca agattaatgc gatgatgggc gacacttttt ccgtcagcag cagccatacg
     7561 atgaccccga taatcagcag tccgataaac gtcagcatca tttctccttt attttatttt
     7621 aaacagaaaa ccgaccgtgc aggcaaaacc gcccacagac gcgggataag ccctgcattc
     7681 tactttttta ttttgaaaca agtcaatcgg tcatttcctc ccatttacgc ctgccgccat
     7741 tcctgcatcc gtccgtcatt tcacagcggc aaccgatacg gaacaaccgg taaatcggta
     7801 tcgggacggc gcgggggcat tcatcccggt gcgccgattc aaacgaaacc gcccctatca
     7861 ttgcggagcg cggggcgtgc cgtacacgcg ggattttata gtggat
//
LOCUS       DAWISC010000091         7869 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWISC010000091 DAWISC010000000
VERSION     DAWISC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.091"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            91..1320
                     /gene="sstT"
                     /locus_tag="WIW87_001358"
     CDS             91..1320
                     /gene="sstT"
                     /locus_tag="WIW87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ5174513.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPFPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATINMAGAAITITVLAMAAAHTQGITVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGIDNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1575..3041
                     /locus_tag="WIW87_001359"
     CDS             1575..3041
                     /locus_tag="WIW87_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ5174514.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEAKEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDM
                     PQNAAGTDSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSA
                     GGQNAGNTAAQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQ
                     NITLTHCKGDSCSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQ
                     MKGINQYIIFYKPKPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGH
                     SGNIFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENG
                     RPYPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDV
                     SGKFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(3117..3332)
                     /locus_tag="WIW87_001360"
     CDS             complement(3117..3332)
                     /locus_tag="WIW87_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174515.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(3325..3540)
                     /locus_tag="WIW87_001361"
     CDS             complement(3325..3540)
                     /locus_tag="WIW87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ5174516.1"
                     /translation="MKEYKVIIYQESLLSSLFFGAAKVNPIKFSEFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(3615..4958)
                     /gene="argG"
                     /locus_tag="WIW87_001362"
     CDS             complement(3615..4958)
                     /gene="argG"
                     /locus_tag="WIW87_001362"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ5174517.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            5271..6071
                     /locus_tag="WIW87_001363"
     CDS             5271..6071
                     /locus_tag="WIW87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ5174518.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAKGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6111..7097
                     /locus_tag="WIW87_001364"
     CDS             6111..7097
                     /locus_tag="WIW87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ5174519.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            7754..7869
                     /locus_tag="WIW87_001365"
                     /pseudo
     CDS             7754..7869
                     /locus_tag="WIW87_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1894 a         1981 c         2198 g         1796 t
ORIGIN      
        1 aatacagccg gaaaataaaa aaaaacacag agaatgagtt ttcttttaaa atacaacatt
       61 ttttaacata tcacaacata aggaagaatt atggcgttcg gcaaatcctt atttcatgcg
      121 atcggccgcg tcagcctggt caggcagatt gccgccggtt tggcgttggg catcgtaatc
      181 ggttcggttt ccccgcaact gggcttggcg gcaggcttgt tcggcagcct gtttgtcggt
      241 gcgctcaaag cggtcgcgcc ggttttggta tttattttgg tggcggccac aatcgcgcag
      301 caccaaaaag gcaacaaggc gcatatcagg ccgattatcg tcctttacct catcggtacg
      361 ttttccgcag ccctgaccgc cgtcatcgcc ggtatggttt tcccgacgca cattgttttg
      421 gcgggcgcgg gcgatgtgtc cgccgcgccg ccttccggca ttgtggaagt attgaaatcg
      481 ctgctgatga acctggtcgc caacccgatt aacgcgattg ccaatgccaa ctatatcggc
      541 attttggctt gggctttggt tttgggcgcg gcgttgcgga atcacggttc ggacgttacg
      601 cggcaggtcg ttgccgattt ggcggaagcg gtttccaccg tcgtgaaatg gattatccgt
      661 tttgcccctt tgggcatttt cgggctggtg tcgtccacaa tcgcggagac gggtttcggc
      721 gcgctggcgg ggtacgcgaa gctgcttgca gtgctgttgg gctgtatggc gtttatcgcg
      781 ctggcggtca atcccgccat cgtgtggtgg aaaatccgcc gcaacccttt cccgctggtg
      841 tttacctgcc tgcgtgaaag tggcgtttat gccttcttta cccgttcttc cgccgccaat
      901 atccctgtga acatggcatt ggcgaaaaag ctgggtttgc acgaagacac ttactccatc
      961 tccattccat tgggtgcaac catcaatatg gccggtgcgg caatcaccat taccgttttg
     1021 gcgatggctg cggcgcacac gcaaggcatt acggttgact tcgccactgc cctgctgctg
     1081 agcctggtgg ctaccgttag cgcgtgcggt gcatccggtg tggccggtgg ctcgttgctg
     1141 ctgattcctt tggcgtgcag cctgttcggt atcgataacg atgtggccat gcaggtggtt
     1201 gccgtcggct tcattatcgg cgtgattcag gattcggcgg aaactgcgct gaactcttcg
     1261 accgacgttt tgtttaccgc tgccgccgat ttgggccgac agaggaaccg ggcggaataa
     1321 accaagctat gccgtctgaa gcccgtttgg cgttcagacg gcatattttt acaattttat
     1381 aggcatacgg cggaaacaat atttattgaa tatgtttcaa cacacagggc ggcacataaa
     1441 gcacagccct atgtgtcgcc ctgatttgga aaggttgcgc tcttcccaaa taaagctgat
     1501 tccaccgccc cgaaggacgg atgtccaagt ggcggggttt aaaccaaaaa ggaaatacga
     1561 tgaaggagat gatgatgttt aaacgcagcg taatcgcaat ggcttgtatt tttgcccttt
     1621 cagcctgcgg gggcggcggt ggcggatcgc ccgatgtcaa gtcggcggac acgctgtcaa
     1681 aacctgccgc ccctgttgtt tctgaaaaag agacagaggc aaaggaagat gcgccacagg
     1741 caggttctca aggacagggc gcgccatccg cacaaggcag tcaagatatg gcggcggttt
     1801 cggaagaaaa tacaggcaat ggcggtgcgg taacagcgga taatcccaaa aatgaagacg
     1861 aggtggcaca aaatgatatg ccgcaaaatg ccgccggtac agatagttcg acaccgaatc
     1921 acaccccgga tccgaatatg cttgccggaa atatggaaaa tcaagcaacg gatgccgggg
     1981 aatcgtctca gccggcaaac caaccagata tggcaaatgc ggcggacgga atgcaggggg
     2041 acgatccgtc ggcaggcggg caaaatgccg gcaatacggc tgcccaaggt gcaaatcaag
     2101 ccggaaacaa tcaagccgcc ggttcttcag atcccatccc cgcgtcaaac cctgcacctg
     2161 cgaatggcgg tagcaatttt ggaagggttg atttggctaa tggcgttttg attgacgggc
     2221 cgtcgcaaaa tataacgttg acccactgta aaggcgattc ttgtagtggc aataatttct
     2281 tggatgaaga agtacagcta aaatcagaat ttgaaaaatt aagtgatgca gacaaaataa
     2341 gtaattacaa gaaagatggg aagaatgata aatttgtcgg tttggttgcc gatagtgtgc
     2401 agatgaaggg aatcaatcaa tatattatct tttataaacc taaacccact tcatttgcgc
     2461 gatttaggcg ttctgcacgg tcgaggcggt cgcttccggc cgagatgccg ctgattcccg
     2521 tcaatcaggc ggatacgctg attgtcgatg gggaagcggt cagcctgacg gggcattccg
     2581 gcaatatctt cgcgcccgaa gggaattacc ggtatctgac ttacggggcg gaaaaattgc
     2641 ccggcggatc gtatgccctt cgtgttcaag gcgaaccggc aaaaggcgaa atgcttgcgg
     2701 gcgcggccgt gtacaacggc gaagtactgc atttccatac ggaaaacggc cgtccgtacc
     2761 cgaccagggg caggtttgcc gcaaaagtcg atttcggcag caaatctgtg gacggcatta
     2821 tcgacagcgg cgatgatttg catatgggta cgcaaaaatt caaagccgcc atcgatggaa
     2881 acggctttaa ggggacttgg acggaaaatg gcagcgggga tgtttccgga aagttttacg
     2941 gcccggccgg cgaggaagtg gcgggaaaat acagctatcg cccgacagat gcggaaaagg
     3001 gcggattcgg cgtgtttgcc ggcaaaaaag agcaggattg atgttgatgc cgtctgaagc
     3061 gagggcgttc agacggcatt gtgatgtttg ggtcgcgggt ccggttcaag tccgcatcag
     3121 ttgataaacc cgtcgtcttt cattttcttt tcggaaaaat actgaccgca taccagtaag
     3181 gcggcgagga tgatgacaaa cagccccgcc atagtcagtg tgattttgaa gaacaattcc
     3241 gcttcgagaa aagaaaacca gagttgcacg atcagcagca gcacccagcc ggcaaacagt
     3301 ccgagacagg tataaacgcc gagcttaaac acgatcccgc tccaaaatga cgacgtaggc
     3361 ctcgcgtttg aaaaacagca gcatacggcg caaatctttt tccatcgtta caacccgcca
     3421 gccttcgggg gtttgtttat tgaggaactc gctgaatttg atggggttga cctttgccgc
     3481 gccgaaaaac aggctggaca acaggctttc ctgataaatg atgactttgt attctttcat
     3541 aggattcctt ttaacataag ggatcatctt ttcagacgac cccttaagtt gctaatgatg
     3601 taaaacagca aaccttattg cttattgccc aactgcggca ataccgaacc ttcgcccagc
     3661 gagagcaaac cgctttgcga gtagataccc agtttgacgc gggtgtcggt gatgtcgagg
     3721 ttgcgcatcg tgagctgtcc gatgcggtcg agcggagtga acgcagcgtc ttcgactttt
     3781 tccatactca ggcgttcagg ttggtaggtc aggttgggcg attcggtgtt cagaattgag
     3841 tagtcgttgc cgcgccgcag ttcgagggta acttcgccgg taacggcttt ggcaacccag
     3901 cgttgtgcgg tttcgcgcaa catcagggct tggctgtcga accagcggcc ttggtagagc
     3961 aggcggccga ggcgcaggcc gttgatgcgg tattgttcga tggtgtcttc gttgtggatg
     4021 ccggtgacca aacgctcgta ggcgatgtgg aacaacgcca tacccggggc ttcgtagatg
     4081 ccgcgcgatt tggcttcgat gatgcggttt tcgatttggt cgctcatacc caagccgtgg
     4141 cggccgccga tgcggttggc ttcgaggaag agttcgacgg gatcggcgta ttctttgccg
     4201 tttagtgcaa ccggcacgcc ttcttcaaag cgtacgctga cttcttccgg gctgacttcg
     4261 acgttttcgt cccaaaaggc aacgcccata atgggtttga cgattttgat gcccgagttc
     4321 aaaaattcca agtctttggc ttcgtgggtg gcacccaaca tattggaatc ggtggagtag
     4381 gctttttcca ccgacatttt gtagttgaag ccgttggcaa tcagaaattc gctcatttcg
     4441 tgacggccgc cgagttcgtc gataaattgc tgatcgagcc agggtttgta gattttcagc
     4501 gcgggattgg tgagcaaacc gtagcggtag aaacgctcga tgtcgttgcc tttgtaggtg
     4561 ctgccgtcgc cccaaatatt cacatcgtct tctttcattg cggaaacaag catagtgccg
     4621 gttacggcgc ggcccagagg cgtggtgttg aaataggcga tgccgccggt ggaaacgtga
     4681 aacgcgccgc attggatggc ggcgatgcct tcgtgtgcca actgcgcgcg gcagtcgatt
     4741 aagcgggcgt tttccgcacc gtattccatc gcttttttgg gaatggcgtt gtagtcgtct
     4801 tcgtcgggct ggccgaggtt ggcagtgtag gcataaggca gcgcgccttt gagtttcatc
     4861 cacaacagcg cggcagaggt atcaagaccg ccggagaagg cgatgccgac tttctgaccg
     4921 acggggaggg attgcagaat ggtatggttt tggctcataa gggtatcctt cggacttgaa
     4981 aaaaggagtt cattatctta taaatataac ccattgtcag ccgttaaggc tgtctgaaaa
     5041 gaagctttgc ccgtttcgct gaaaatcttt tcagacggtc tttacgccat cctcaacctg
     5101 actccctccc ctgcggggga gggttgggga gagggtacag acagcaggcc ttaagcaaac
     5161 gctgcctgaa aaacgcaagc tcgaccctct ccctaaccct ctcccacggg agagggaaca
     5221 aatcacagca ccatcgacat tttcagacgg catcggtaaa gaaaccatct atgaacgcgt
     5281 tcaacctcat catcatcggc gacgaaatct tacacggcag cagccaagac aagcatttcg
     5341 cctttttcaa atccctgctc gaatccaaag ggctgaagct caatcaggtg caatacctgc
     5401 ccgacgaacc cgatttgctg gtcaggcaac tgcgccgcag cttttcagac ggcatgccaa
     5461 ctttcgttac cggcggtatc ggcgccacgc ccgacgacca cacccgccaa gccgcagccg
     5521 ctgctttgga tttgcccgtc gtccgccatt ctgaagccgc caagtttatc gaaggcataa
     5581 cccagaaacg cggcgagccg cttgattcgc cggaacacgc ccaacgcctg aaaatggcag
     5641 attttcccga aggcgcggaa ttggtgccca atccgtttaa caacatcgcc ggattttcta
     5701 tccgcgagca ttatttcttc cccggctttc ctgtgatggc gcacccgatg gcggaatggg
     5761 tattggaaac ttattacgcc gaccgcttca accaaaccga acgcggcagc cgcagcgtgt
     5821 atgtgtttga gcagcccgaa tcgcgcatta cgccgcttat ggagcatatc gagcaaactt
     5881 atcccggcgt gcgttcgtac agcctgccca gcgtcggttg gacgcattca gacggcacgc
     5941 aggtcaaacc gcacattgag ttcggcatca aagccaaagg agaagccgtc aaccttttgg
     6001 acgcggcgtg ggcggaagtg ttgcacagct tggacggatt gggagcagag ctgaaaaatc
     6061 gggtaaactg aaaaccgttt gcccaaatcg gacaatattt aaggaaaacc atgcaatacc
     6121 gaatcagaag agaaaacgaa gcacccgaag caaaaaatgc gggggaaacc ttatgggagc
     6181 gcgacatcat gcgcgaagtg ctgttgtccg cctatcagga caggcgcagg gagcggatgt
     6241 ggaaaaacat ctggcgcgcc gtcagcaccc tgattctggt tgccctgatt gcaggcattt
     6301 tccgaaaaga cgaagccgca ttgcagttgg cgggcaacac gccgcatacc gcagtcgtca
     6361 atctgtacgg cgaaatcggc aacggtgtag aagaccaggt caaaaaactc aaagacggta
     6421 tggaggccgc ctacaaaaac ccgcaggcaa aagccatcgt catccgcgcc aacagccccg
     6481 gcggttcgcc cgtcgtgtcc aacaccgctt ttgaagaaat acgccgtctg aaggcgcagc
     6541 accccggcat tcccgtttat ctcgtggcgg aagatatgtg cgcgtccggc tgctactaca
     6601 tcgcggcggc ggcggacaaa atctatgccg acccgtccag catcgtcggc agcatcggcg
     6661 ttatcggcag cagtttcgac gcgaccggcc tgatggaaaa aatcggcgtg aaacgcaggg
     6721 ttaaaattgc aggcagcaac aaaggcatgg gcgatccgtt ttcgcccgaa acgcccgaac
     6781 agtcaaaaat ctgggaagaa atgctgaccg gcatacacgg cgagttcatc aaagccgtca
     6841 aaaccggacg cggcggccgc ctcaaattcc gacaatatcc cgacgtgttc agcggtcgcg
     6901 tgtacacagg cgcggatgcg ctgaaagtcg ggctggtgga cggactcggc aatatttaca
     6961 gcgtggcgcg agacgtggtc aaagcccccg atgtggtgga ttacactccg aaggacgatt
     7021 tcggcagaat cttgggcaga cgcttcggcg cagaactcaa agcaagcgtg cgcgaagccc
     7081 tgcaggccgt ccggtagcgt tgtttacagt atttctaagg gaataccaaa ataaaaatat
     7141 acacaaaaac actcacctcc ccagtatgct gccactaagt cagccgcgtc actatttacc
     7201 gtgcacttaa agccgtaaaa ggcaggttgt ccccgggagg ttttgtgcat cctgataccg
     7261 atcggttgtt gttgctcaaa ggacagaagg ccgctgataa acgagattac ctgtttgtcg
     7321 ctattgacga tttttatact ctgccatttt gccggacaaa accgcagaca gtgctgccaa
     7381 gtttctgacc gaacatctgt ccgacccctg cttgtacctg attgagtacg tttactctga
     7441 caatgatagg taatataaag agccgtccaa catgctttcg gtgcagcttg ttatgataat
     7501 gggattggtt ggaggcttgc ccgatttgct tgtccgcaga ccaacggtaa ggcggagcgg
     7561 gttatccgta ccttgatgga gatgtggcat gaggaacagt cgtttgacag tccggaacat
     7621 cggcaaaagg agttgtgctg ctttattaat ttaggggctg ccctagatca ctagggaaat
     7681 tcaaattaag ttagaattat ccctatgaga gaaagtcgtc taagacggta taaacaaaat
     7741 aaactcattt tgttatcgtg gctatgatgt attggatgcg ggcgaattta gccatttccg
     7801 tatcaatcac agcacacatt ttgccgaacg acaaaaccat attaatggaa ctgagaactt
     7861 ttggaatca
//
LOCUS       DAWISC010000092         7864 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWISC010000092 DAWISC010000000
VERSION     DAWISC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.092"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..1334)
                     /locus_tag="WIW87_001366"
     CDS             complement(<1..1334)
                     /locus_tag="WIW87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ5174520.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1408..2748)
                     /locus_tag="WIW87_001367"
     CDS             complement(1408..2748)
                     /locus_tag="WIW87_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ5174521.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            2891..3517
                     /locus_tag="WIW87_001368"
     CDS             2891..3517
                     /locus_tag="WIW87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ5174522.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3581..4999)
                     /locus_tag="WIW87_001369"
     CDS             complement(3581..4999)
                     /locus_tag="WIW87_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5174523.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMVAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5326..6054
                     /locus_tag="WIW87_001370"
     CDS             5326..6054
                     /locus_tag="WIW87_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ5174524.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6113..6601)
                     /locus_tag="WIW87_001371"
     CDS             complement(6113..6601)
                     /locus_tag="WIW87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ5174525.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7359..7823)
                     /locus_tag="WIW87_001372"
     CDS             complement(7359..7823)
                     /locus_tag="WIW87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ5174526.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2030 a         2120 c         1973 g         1741 t
ORIGIN      
        1 cccgacaggc ggatttgaag tttctccaca tatccggcgc gttcggcgcg gctggtttcg
       61 gggcggtgcg tattgagcga cgaccatttc caatgactgc gggctttgtt gagttcgggc
      121 gggagtctgg cggcgtccca cggggctttg cggctgtgca gctcgatatc cgactgtgcc
      181 gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg cgcgggcggc gagcagggtc
      241 agggtttcag ggtcggtgtg cagggtttgg cggccggcga gtttgtcgga aatggtgcgg
      301 gtattgggca ggatgccgcc caaaaagccg ccgattgccg tacccaatcc gagcgagccg
      361 ccgagtgtgg cgatgtccag ccccaagccg atgagcgcgc cggttgccgc gcccgtgccg
      421 gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg gaaggcttgc
      481 ggcatccagt cgccgccgtc gatttcgctg tggtagaaac ggtagagggc aaacagccgc
      541 tgctgcatct gccgttcgag ttggcgtatt tccgcctgca tggtttgcag cacggtggcg
      601 gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg catcgagcag gaaatcggcg
      661 atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc gccggcgcat ggctgtcagg
      721 cggtcgagtg tgctgcgttc gggcaacatg gtggcgaggt tttcccacag gcgcagttcg
      781 ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt tctcctcgcc
      841 agcatggttg tccacgattc gggaggctgt ccgccggtaa agttgaacac gggcataacc
      901 ggtttggcgc accatgaaag gataatcaat tcatcccgat atttgtcgag aacgggttcg
      961 cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga cttgccgtaa gactttggct
     1021 tcctgattga aatcatggtg cgcgccgtgg ctgccgagaa actgttgcag ccgttcgatg
     1081 ccgtctgaac gattgtccgt atggttttcc agccattcca aaacaccgcc cgcgtcttcg
     1141 agtccgggcg tgtcgtacag gaaaaccagc gtgtctgcgc cgtcgctgat ggcggcttct
     1201 tcgacatgac gcgtggtcga tggggcgttt ctgacttcgc cgaaaccgct gtcgcgcaac
     1261 agggtacgca ggagcgaggt tttgccggtg ttggtgtgtc cgacgacggc gagggaaagg
     1321 ggttgtttgt tcatgatgtt tttgaagaat ggattttcag acggtctttt ttcagaatgg
     1381 cggcttaaca gaacatttca agtgagttta ttggtctttc aaacggcctt cctgcgccgc
     1441 cctgtcaggc tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag
     1501 cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc
     1561 ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg gcacagtttg
     1621 ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcgcggc
     1681 aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc
     1741 gaaccattcg ccgtcctgcc attcggtctc cagcatgacc gcccatttcg gcgcatcgtt
     1801 caagacgatt ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg catcggtgat
     1861 tttgttctgc cagcggcgga tgaccgcctg ataatagggt ttttccaaat ccaatccgtt
     1921 ttcgcttgtt ttcaaaagga ttttgcatac cgcccaagcc aagaggcgcg gcaggatgcc
     1981 gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc
     2041 gttcagacgg ccttcgatga ccgcccgcgc atcaggcacg ggaaaaccca gtttcgcagg
     2101 cagccatgcc aacatttcca ccagccgtac cgaagacgaa tcgcccaaca gcgtgctttc
     2161 ccagttgaac gtatattgcc gcaccaaaag cagcaacaat accgacacca gcattccgag
     2221 cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta ccgaaggttg
     2281 ccgccactcg tccgcataca gccgcaacac cgcctgattt acagggtctt tgccccgaaa
     2341 ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca acattgccaa
     2401 ccatactgcc agcatcagcg tattcatgcc caacacgccc gccaaaacca aaaagaaatt
     2461 cagaccctga ttgtccatta gaagataagt gcctgaaaaa ccggtaaaaa atgcaaacgt
     2521 cgccgccacc acccacaacc agaacgaccc cgcacgcaca cgttccaacg tctcccgcag
     2581 catacggttc ctgtcaatca tctccgcccg acggatgatt ttttcctccg tactgccgtc
     2641 cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa aaccgccttc
     2701 gtccaaaata cggatcagct caaccagttt tcgggatgga ttcaacataa aatgccgtct
     2761 gaaaataaaa aacagatttt aacacacgca ttttcaagaa tattcacagt gtaggcaaag
     2821 agtaaatctc acacagaagc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa
     2881 agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga gcggtttgat
     2941 tctggcggcg gcaggtttgg cgctgatgct gctgaaaacc ataggacacg gggacggcta
     3001 ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc
     3061 gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg
     3121 catgatttat gtgctgattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa
     3181 cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca
     3241 agaactcacc atcggacgga aaagcgaaaa acgactgctg tctattgcga tttatgtcgt
     3301 catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgctggcagg
     3361 actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa
     3421 cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat
     3481 cacccaattt gtcagtgtgt acggttatgt aatctgaatg ccgtctgaaa agcaaaacct
     3541 cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg tcgtggaact
     3601 cgtgaaattc atactgatag gacaaatccc gacccgcttt tttctgtgcc aaataatcat
     3661 cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc gggataacca
     3721 taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg agcgtaccca
     3781 aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc ctgaaaagaa
     3841 aacggatgtt ggactcgccg aaataatacc agccgataat ggtcgtcaag gcgaaaaagg
     3901 tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta aaggcaaatt
     3961 gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc agtcagcagg ataatcaatg
     4021 ccgtagccgt acataccaaa atcgtatcga taaacacacc gacaaatgcc accatacctt
     4081 gctgcacagg atgcttcaca tccgcagtcg cgtgggcgtg cggagtcgaa cccatacctg
     4141 cttcgttgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcgcgc ataccgatac
     4201 ccgcagcacc gcccaaaacg gcttcgggat tgaaggcggc ggtaaagatg tggttgaaca
     4261 tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa atataaacaa
     4321 ccgccataaa cggcacgaca aactgggcaa tattggcaat acggttcacg ccgccaatca
     4381 caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc acatcaaatg
     4441 caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata aagcccaatg
     4501 cgataatcaa cgcaatggag aaaaagcccg acaggaaacg cgccgcgccc ctgccgattt
     4561 tcggagtcag accgtgcgta atataaaacg ccggcccacc gatgtatttg ccgtggctga
     4621 cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc cccaaaacgg
     4681 cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg gtcgccacgc
     4741 cggcgacgtt gcccgtaccg atttgcgcag atatggcaac cgccaacgcc tgaaactgcg
     4801 ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg gatttgaatc
     4861 ccgcgcccag cttggtaatc tgcggcgcac cgagatacag cgtaaaaaac aggccgatac
     4921 ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta cccaccagaa
     4981 cagacaatat attttccata aaataaacct tatcttacga ttaaaatgac tgctttcccc
     5041 aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca gatgccttaa
     5101 attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt aattaaaaca
     5161 tttctttatt tttaagcgga aagcggggga aatcgttttc agacggcatc gacaacggca
     5221 cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg accgtaaaaa
     5281 atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc aggccatagc
     5341 aagtgggcaa atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg caaaatcttt
     5401 acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga ccccggttca
     5461 aatccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc caaagacaat
     5521 gtgcaacgcg ccatcgacaa aggcacgggc aacttggaag gcgtggaata catcgagttg
     5581 cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct gaccgacaac
     5641 aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg caacttgggt
     5701 accgacggct gcgtggcgtt caacttcgtg catcagggtt atttggtatt cgaacctggc
     5761 gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcagaaga cgtggttacc
     5821 aacgacgacg gttcgattga agtcattacc gcgccaaacg attgggcggg cgtaaaatct
     5881 gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg cgcccaaaac
     5941 gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga cgcgctggaa
     6001 gacttggacg acgtgcaaga cgtttacact tccgccgtat tgaatctgga ctgatacgca
     6061 gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg atttatttag
     6121 cccttgccca cgcccaccaa accgtcagga caaccgccag aaaatacgcc gcgctccaaa
     6181 caggcaaagc aacccctaac agataatccg gttcggcaca atttccgaac ccgcgcacga
     6241 caggctcgaa ccaatcaaac aaaggccagc ccttcaatcg aaacgtccac ggcgcaccgc
     6301 acgaaggagc cgtacccggc ggcagcgact gcaaccacaa ctgatatgcc gcaacagaaa
     6361 tacccgtaac ggccggaata ctgataaaga cagcaccgaa caaaccgcct gcccttcctc
     6421 ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg cataaccgct
     6481 gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc aaagaaccgg
     6541 caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat aacggggtca
     6601 ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat atcccgagat
     6661 ggcaagggac gaatcgacac cggcgcaggt gcgggcgatt tcggttttcc cgatatgcac
     6721 aaaacctgac aagggacaaa acggcgattt gcccgcacaa tgcccacaaa aaaatacatc
     6781 cggaggattt gaatttcagc aaatttagcg aatcaaaagt ttatttcaat gaaatcatat
     6841 gatttttttg aataagcgga ttgatgggtt tttgaaggaa tttgttaccg gatagccatc
     6901 gggcaagttt tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat aattgacagc
     6961 ggataagaaa cggcggataa ttcccgccgt cgagttgctt gatgcagctt gatttttctc
     7021 ctctatttct cctttgtaga cttggcacac attcaactgg atgtgtgcat ttttttatct
     7081 ccgctttttt tgaaatttaa ttacttttat tgtttggaat tccattcttt aagaaattta
     7141 acaagaaaaa cgcttgcttt ttgaccggaa aagctgcata attcattccg ttagctggtt
     7201 cgagtagtca gttaatagtt tctcctctat ttctcctttg tagacttggc acacattcta
     7261 ttgaatgtgt gcattttttt atctgaagca acaagcctct gtgcgtgatg ttgttatgtt
     7321 tcatttaggt gtcaaaccgc atatccggtc tgaaatattc aatccaaatc caaaaccgga
     7381 ttttctttga cctcctccat cacaacataa ctcctactct ccgaagcggc aggcagttgc
     7441 aataagatat tgcctagcat atcccgatag gcagacatat cgggcaaacg tactttaatc
     7501 agatagtcgt attcgcccga caccaagtgg cattccataa tttgcggaat tttcagcact
     7561 tcttttttga aatcttcgaa aatattgccc gatttggatt gcagcttcag ctcgacaaaa
     7621 accaatagcg gtttgcccaa cagatgggga ttgaggtggg cgtgatagcc ggaaatataa
     7681 tgttcccgct ccaaacggcg caccctctct gtaacgggcg tggtggacaa gcctaccttc
     7741 tcggcaagct ccgtcatcgg gatgcgggca ttctgttgga ggatcttaag aatgcggaaa
     7801 tcgattttat ctagttcttt catttggatt gccttgtatt tattattgat tttaacaaat
     7861 agag
//
LOCUS       DAWISC010000093         7799 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWISC010000093 DAWISC010000000
VERSION     DAWISC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.093"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(453..2489)
                     /locus_tag="WIW87_001373"
     CDS             complement(453..2489)
                     /locus_tag="WIW87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ5174527.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
     gene            complement(2577..3449)
                     /locus_tag="WIW87_001374"
     CDS             complement(2577..3449)
                     /locus_tag="WIW87_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ5174528.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            3719..6109
                     /gene="gyrB"
                     /locus_tag="WIW87_001375"
     CDS             3719..6109
                     /gene="gyrB"
                     /locus_tag="WIW87_001375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ5174529.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6206..7591
                     /locus_tag="WIW87_001376"
     CDS             6206..7591
                     /locus_tag="WIW87_001376"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ5174530.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7662..7760)
                     /locus_tag="WIW87_001377"
     CDS             complement(7662..7760)
                     /locus_tag="WIW87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ5174531.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1782 a         2162 c         2194 g         1661 t
ORIGIN      
        1 ccgtctgaag cgttgtttga ccgtttcaga cggcattttt atcaaattca aaaatattcc
       61 gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgat
      121 aagtttccgc accgacagac ctggattccc gcctgcgcgg gaatgatggg atttctgttt
      181 ttgatttttt tgtttttggg gaatgacggg atttgagatt gcgggcattt atcgggtaaa
      241 acggaaatta aacgttgcga aaatttatcc ggaatcacag caacttttcc gcgtcattcc
      301 cacgaaagtg ggaatctaga acgtaaaatc taaagaaacc gtttttcccg ataagtttct
      361 gcaccgaccg gtctggattc ccgcctgcgc gggaatgacg gtgcggatgt ttttctatcg
      421 aatccgccat attttttact tcaaccctgc cgtcagaccg cgttgtcgaa accgctgtgg
      481 cgcaagagtg cgtctatgct cggttcgcgg ccgcggaagg ctttgaagga ttctgccgcg
      541 ctgcgcgatc cgccgacggc gaggatttcc tgccaaaagc gtttgcctgt ggcggcgaca
      601 tcgtcgcttt cttcaaaggc ggcgtatgcg tccgcgctca atacttccgc ccacgcgtag
      661 ctgtaatagc ctgcggaata gccgcctgcg aagatgtggc cgaagctcaa ggcgaagcgg
      721 ttgtattcgg gcggctggat gacggcgact tttttgcgca cgctgtctaa aacctgttgc
      781 cagtttttca gacggccttc gtcgtcttcg ctgtaaatca tcatatcaaa gagggcgaac
      841 tccatttgcc ggacgaggaa catgccgcgt tggaagtttt tggcggcgag cattttgtcg
      901 aagagttctt tcggcagggg aacgccggtt tcttcgtggg ctgacatttg tgccaagaca
      961 ttgtattccc aaacgaaatt ttccataaac tggctgggca gttcgaccgc gtcccattct
     1021 acgccgttga tgccggatac gcccagttcg tccacttggg taagcaggtg gtgcagcccg
     1081 tgtccggttt cgtggaagag gatgaggatt tcgtcgtggc tcaggcgggc ttccctgccg
     1141 ccgacgggtg gggcgaagtt gcagacgagg taggcggtgg gcagttgcag cgtgccgtct
     1201 gaaaaacggc ggcggccttt gtagtcgttc atccacgcgc cgccgcgttt gccttcgcgt
     1261 gcgtacaaat ccatataaac gccgcctatg gtttcgccgt tttgttgcaa ttcaaaatag
     1321 cgcacgtctt tgtgccagac ggggacggtt ttttcggtaa atccgatgcc gtagagtttt
     1381 ttgatttggg cgaacagtcc gtttaatact ttgccgacgg ggaagtattt tttgacttcg
     1441 gtttcgctga acgcgtattt ggcttcgcgc agtttttcgc tggcgtagcc caagtcccac
     1501 ggttgcaaat cggcgaggtt caggctttcg cgggcgaagg ctttgacttc ggcgaggtct
     1561 ttttcggcgt agggtttggc gcggcgggcg aggtcgtgca ggaagtttaa aacttgttcg
     1621 ggcgtgtccg ccattttggt tgccagcgac aattcggcgt agtttttgaa gccgagcagt
     1681 ttggcggttt gcagggcgtt tgcgagcgtg cggtcgatgt tggcggtgtt gtcgaatttg
     1741 ccgtcgtctg aaagttcgct ggcgcgggta acgtaggcgc ggtagatttg ttcgcgcagt
     1801 tcgcggttgt cggcgtattg gatgacggcg aggtagtgtg gaatctgcaa gccgattttg
     1861 tagcctgttt tgctttcgct ttgcgcggcg gcggcaaaca tggcgagcgc gtcttcggga
     1921 atgccggcaa gcggtgcggc atcgtcaaag taaatgccga acgcgtcggt cgcgtctagg
     1981 acgttttggg agaatttggc ggaaagttgc gcgccttcgg tttgcagttt tgccagttct
     2041 gcctgctgtt cgggcggcag ttccgcgccg ctgaggacga aatcgcgcag atcgtggttg
     2101 agtttggttt tttgtgcggg ggagagggtg tcgaattcgg gggaattttt gatggttttg
     2161 aagcggttgt acagctcgat gtcttgtccg atttcggtga agaagacggt gatttcgggc
     2221 atcagttcgt tatagacggc gcgcagttcg ggcgtgtcgg cgacggagtt gaggtgcgac
     2281 accacgcccc aaatcctgcc gacgcgttcg gtgatgccgg tcaggggttc gacagtgttt
     2341 gcccagccgg tgtgcgtttg ggctttgatg gcggcgattt gttcgcgcgc ttcggcgatg
     2401 gcggtttgca gggcgggttt gatgtcttcg gttttgattt gatcaaaacg gggttcttcg
     2461 cccaaatgga gcagtgcgtt gtcagtcata agatgggttt cctttgcgtg ttgtgtttca
     2521 gacagcatat cggcaatatg ccgtctgaag ccggaaatgg ggtcggtcgg ggaaagttaa
     2581 agtgtttcgg agccggcggg gtgatttaac aggcggtatt gtacgcgcaa aaattcgtcc
     2641 ataaccgcct gttggatttc cagccgcttt gaaacggggg aagcgaagcg gacgatgatg
     2701 cggtatgcct tgtcatcgta cggcacgcgg gtaacgcgcg gtctggcggc gggcgtgata
     2761 aacagttttt ccgcctgcac gttttccaaa tgccgttgga tggcggggat gtagggcgcg
     2821 cacaagggct cgagtacggc tttcagacgg catacggctt catccgaatc caaatggatg
     2881 ggaacgggga tttcgaccgt atggatgaca tagtcgccca aaatattgtc gcggcgcacg
     2941 gggtggctca acaacaggct gttggggaaa gaaacggtgg ttcccgcaag ctgtccgacc
     3001 aaggggttcg gaccgacctg catcatcagc gtgttcaaca ggttgatgtc gaccacgcgc
     3061 ccgcgcaggc cgttgatttc gatatagtcg ccgaccgagt attgctgggt ggcagacctt
     3121 aaaatactgc ccgacagaca cataatcagt tccttcgtcg ccacgacgac cgccgccgcc
     3181 accgcaaaca tcgacaaagc cagcgtttgg atttgcgccg accagataaa tgccagcgaa
     3241 aacagcacca aaagcagcgt tatattgcgg ctggcaacca aaaaccgccg cttgctttcg
     3301 atgccgaaat ccggatgccg tttgaagtgg atattcaaca gaagggcgcg cgccagcagc
     3361 aaagccgcaa ccgccgccac ggattcgacc gcctccgcac gtatcgggac ggcaccgagc
     3421 caagtgtcca acatattcca tatttccatt tccgcgcccc tgtccgaaaa gtatagggtg
     3481 tagattttag tggcaaaaaa acgcttttgc cactttacgg gacaatcccc gacctgatgc
     3541 cggaggcaaa cgtaccgttt catcttttcc cggcggcaca ctgatgcgga taacgctgaa
     3601 ttaggcggca aaatcggcta taatacacaa ttatatatgt cgtcccgccc gctttttcta
     3661 caaggtgcgg acttgttttg gcggcatcgc aaaatctttt caaaatccgg caaaaaatat
     3721 gactgaacaa aaacacgaag aatacggcgc cgacagcatt caggtgctcg aaggcttgga
     3781 agcggtacgc aaacgccccg gcatgtacat cggcgacacg caggacggca gcggtctgca
     3841 ccacatggtg ttcgaagtat tggacaacgc cattgacgaa gcactcgccg gacattgcga
     3901 caaaatcacg gtaacgatac acgccgacca ttccgtcagc gtcgccgaca acgggcgcgg
     3961 tatgcccacc ggcatccacc cgaaagaagg acgctccgcc gccgaagtca tcatgaccgt
     4021 attgcacgcg ggcggtaaat tcgacaacaa cagctacaaa atctccggcg gcctgcacgg
     4081 cgtgggcgtg tccgtcgtca acgcgctgtc cgactgggta acgctgacca tctaccgcga
     4141 cggcaaagaa cacttcgtcc gcttcgtgcg cggcgaaacc gaagagccgc tgaaaattgt
     4201 cggcgattcc gataaaaaag gcacgaccgt gcgcttcctc gccagtacgg aaaccttcgg
     4261 caacgtcgaa tacagcttcg acatccttgc caaacgcatc cgcgaacttt ccttcctgaa
     4321 caacggcgtg gacatcgaat tgaccgacga gcgcgacggc aaacacgaaa gcttcgccct
     4381 ttccggcggc gtggcgggtt tcgtgcaata catgaaccgc aaaaaaacgc cgttgcacga
     4441 aaaaatcttc tacgcgttcg gcgaaaaaga cggcatgagc gtcgaatgcg cgatgcaatg
     4501 gaatgacagc tatcaagaaa gcgtgcagtg tttcaccaac aacatcccgc aacgtgatgg
     4561 cggtactcac ctgaccgcac tgcgccaagt gatgacccgc accatcaaca actatatcga
     4621 agccaacgaa gtcgccaaaa aagccaaagt ggaaaccgca ggcgacgata tgcgcgaggg
     4681 tttgacctgc gtgttgtccg tcaaactgcc cgaccccaaa ttctcgtccc aaaccaaaga
     4741 caaactggtt tccggcgaaa tcggccccgt tgtcaacgaa gtcatcagcc aagccctgac
     4801 cgacttcctc gaagaaaatc cgaacgaagc caaaatcatc accggcaaaa tcgtcgatgc
     4861 cgcccgcgcg cgcgaagccg cccgcaaagc acgcgaaatc acccgccgca aaggcgtgat
     4921 ggacggcttg ggactgcccg gcaaactcgc cgactgccaa gaaaaagacc ctgccctgtc
     4981 cgaactctac ctcgtcgagg gcgactccgc aggcggttcc gccatgcaag gccgcgaccg
     5041 caaattccaa gcgattttgc cgctcaaagg taaaattttg aacgtcgaaa aagcacgttt
     5101 tgaaaaaatg ctggccagcc aagaagtcgc cacgctgatt accgctttgg gcgcgggcat
     5161 cggcaaagaa gaattcaatg ccgaaaaact gcgttaccac cgcatcatca tcatgaccga
     5221 tgccgacgtg gacggcgcgc acatccgcac cctgctcctg accttcttct accgccaaat
     5281 gcccgagctg gtcgagcgcg gctacatcta tatcgcccag ccgcctttgt ataaagcgaa
     5341 atacggcaaa caggaacgtt acctcaagga cgagttggaa aaagaccaat ggctgctcgg
     5401 tcttgccttg gaaaaagcca aaatcatttc agacggccgc accatcgaag gcgcagaact
     5461 tgccgacacc gccaaacaat tcctgttggc aaaaaccgtc atcgaacagg aaagccgctt
     5521 cgtagacgaa ctcgtcctgc gcgccatgct acacgcgtcg cccattgatt tgacgtcgtc
     5581 tgaaaacgcc gataaagccg ttgccgaact ttccggtctg cttgacgaaa aagaagtcgc
     5641 cctcgaacgc atcgaaggtc atgaaggaca ccggttcatc aaaatcacgc gcaagctgca
     5701 cggcaacgtc atggtcagct acatcgaacc caagttcctc aacagcaaag cctaccaaac
     5761 cctcacccaa accgccgccg cgctcaaagg catggtcggc gagggtgcca agctttacaa
     5821 aggcgaaaac gggtacgacg cggacagctt tgaaaccgct ttggacatct tgatgagcgt
     5881 tgcccaaaaa ggtatgtcca tccaacgata caaaggcttg ggcgagatga accccgagca
     5941 gctgtgggaa accacgatgg atcccgccgt gcgccgcctg ttgaaagtgc gcatcgaaga
     6001 tgccattgcc gccgacgaag tgttcgttac gctgatgggc gacgaggtcg agccgcgccg
     6061 tgcctttatc gaaaacaacg cgctgattgc ccaaaatatc gacgcataag tgccgtttta
     6121 aaaaaggaga cgggcatcgt gccgcgtctc ctttttggtt ggtcaaacgg aacctgtgcc
     6181 gtctgaaaaa ccgtcggagc aaaatatgat cagcattttc gatattttca aaatcggtat
     6241 cgggccttcc agttcgcata cggtcggccc gatgaaggca gccgccgcct ttgcggcagg
     6301 tttggatgca caggctgttc gcatcgtcat cgacatttac ggctcgctcg cactgaccgg
     6361 atacggacac ggtacatttg acgcgctgat gctcggtttg gaaggcagcc tgccgcacga
     6421 catcccgctt gccggcattc ccgaacgcct cgaacgcatc cgcacgcagc acatcctccg
     6481 gctcaacggg caagaaatcc gcttcatccc cgaccgcgac ctgaacatac tcggcaatca
     6541 agtgctgccc aaacacccca acagcctgcg ttttaccgcc tatgcttcag acggcacggt
     6601 attgaatgaa caggtttatt attcggtcgg cggcggcttt gtcgttaccg aagaagattt
     6661 tgaccggcag gcggaaacgg aaaaagccgt tccctatccc tataccagtt gcgccgaact
     6721 gcttgcccga tgccgtctga accggctcga catctccgaa gtcgtgttgg caaacgaagc
     6781 cgcgcttgcc ggatgcggcg aagccgaaat ccgccgccgc gccgctgccg ttgccgaggt
     6841 tatggaaggc tgcatcaaac gcggcttggg tgcggacggc gaactgccca gcggattgaa
     6901 cgtccgccgc cgcgccccgc agcttgccgc caagctcaaa gtcctgcgcg aaaccgaaat
     6961 cgtcaacacc cagctctggc cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc
     7021 cggcggacgc gtcgttaccg caccgaccaa cggcgcggca ggcatcattc ccgccgtatt
     7081 gcactatttc cgcaagttca atccgcacgc cacacaggaa cgcgtcgaaa acttcctgct
     7141 caccgcaggc gcaatcggca tcctctacaa gaccaacgcc tccatttccg gtgcggatgt
     7201 cggctgtcag ggcgaagtcg gcgtagcgtg ttcgatggcg gcgggcgcat acgccgaagt
     7261 catcggcggc acgcccaaac aagtggaaaa cgccgccgaa atggcgatgg aacaccattt
     7321 ggggctgact tgcgaccccg tcggcggact ggtgcaaatc ccctgcatcg agcgcaacgg
     7381 catcgccgcc gaaaaagccc tcaaactcgg cacgctcgcg cttttggaag acggcacgga
     7441 caaaaaagtc tcgctcgacg aagtcatccg aaccatgctg caaaccggcc gggatatgaa
     7501 ggcgacctac aaagaaacct cgcttgccgg actcgccgcc acgctccgga aaaaagccgt
     7561 ccccgtatcc gtgcgcgtgg tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga
     7621 aaacagtttt ttagacggca tttcatttat ttcttgcggc tttaccgttt cagtgccagc
     7681 gtcaacacgc ccgccgtaac cagccccaag cctatccatt cctgcgtgtt cgggcgttcg
     7741 tctaagaaaa ccaccgccat caggaatgca aaccggcgtt gtttgcagca tccggcaag
//
LOCUS       DAWISC010000094         7681 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWISC010000094 DAWISC010000000
VERSION     DAWISC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.094"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="WIW87_001378"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="WIW87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ5174532.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(577..1368)
                     /locus_tag="WIW87_001379"
     CDS             complement(577..1368)
                     /locus_tag="WIW87_001379"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ5174533.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLDDEEKMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1417..1824)
                     /locus_tag="WIW87_001380"
     CDS             complement(1417..1824)
                     /locus_tag="WIW87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ5174534.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLTGWCKPRKPAA
                     IDFWDIGGESPPSLGDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1824..2510)
                     /locus_tag="WIW87_001381"
     CDS             complement(1824..2510)
                     /locus_tag="WIW87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ5174535.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2630..3673
                     /locus_tag="WIW87_001382"
     CDS             2630..3673
                     /locus_tag="WIW87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ5174536.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="WIW87_001383"
     CDS             3836..5062
                     /locus_tag="WIW87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ5174537.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5348..7498
                     /gene="yccS"
                     /locus_tag="WIW87_001384"
     CDS             5348..7498
                     /gene="yccS"
                     /locus_tag="WIW87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ5174538.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAILLFQIVLPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1889 a         2568 c         1667 g         1557 t
ORIGIN      
        1 ttgtttgccg ccatcagtcg gcaaactgtt ttttcatcct ctctcggcgt tcttgggact
       61 caacagataa agtggctgtc gggcgtgcca gcagccgctt caaaccgata ggctctcccg
      121 tatcggcaca gaatccataa tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt tcgttcccga tcgcgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gtcgtagcgg
      301 aagcattttc gatgagttcg tcttgcattt ttaccagcaa ttcgcggaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc cgctccaatt caaaatatct tgttctgtca
      421 gctttgccat aatgtatatc ctctcggttc accgcaaaaa acaaagcacc acaaagtggc
      481 tttattcctt tgatttttat aaggcttatt gcctaaggcg tttttgaaaa cgcgcatcat
      541 aacacgtttt ggattggttt ttttattttc tttacattat tgatattgcc gttccatttc
      601 ctgcgaacgg cgcacacagg cacaaacgcc ctcgcttatg gcttcggcga cacgatgccg
      661 cctgaaagct tccacggctt cgtgggttgt cccgcctttt gacgttacat ttttttgaag
      721 cttctcgaaa tcttcacccg tctgctcggc aagggcaacc gctcctttaa acgttgccag
      781 actgagcgcg cgtgcttctg ccatatcaaa cccttgtcgg atggcggcat tttgcaatgc
      841 gtccagcaga taaaacacat aagccggtcc gctgccgctg atgccggtaa tgccgtgcat
      901 tttttcctca tcatccaacc aaacagtcaa accgactgat ttcatgattc gatcggcaat
      961 cctgcggtct gtttccgata cttccgcttc ggcatacata ccagatacgc ccagcccgat
     1021 ttttccgggt gtattcggca taacccggac aatgcggcgt gttcccccga ggtaacggct
     1081 gagcgtaccg accgacaatc cggctgcgac agaaagcacc aatgcgccgt tggtgcggat
     1141 atttttgcac gcagcttcca tatcctgcgg tttgacggca aggattaaaa catcgtcgga
     1201 atgaagctcc ggcagggttg ccgaagtttc gacccccaac tctttttcca aacgttcgcg
     1261 tttttccgca ccccgattgg ctatatagat gcggtaaccg ccttgtttga ccaatccgcc
     1321 cgcaacggca gccgccatat tgccgccgcc gagaaaataa acattcatta tttctaccct
     1381 attcaaaatt accttactaa attcattccc aattttttac catcggacgc gctcggacag
     1441 accgttgcgc cgcaacccct gcttttccaa gcagtcaaat cctccctgtt tgaacgtctc
     1501 aatcaaaggt ttgccgtcac gcgtacgcca atccagcccg caggcttcaa acttccctat
     1561 tttcctgtaa aagtaagatt gttgtgcgga ttcatattcg tttgccctga cggaacgatt
     1621 gccgtctgaa agcggtatct cgtagtcccc taaagacggc ggactctcgc cgccaatatc
     1681 ccaaaaatcg atggcagccg gttttctcgg cttacaccag ccggtgaggg acgaacactc
     1741 ataccacccc gccagccggc agcctgcaac cgtaaatgca agcaacgcag ccattgccgt
     1801 atattttatt gcccgaattt ccatcagccc cttttcccga aaatcgcgct gccgatacgg
     1861 acgtgtgtcg caccgcactc aatggcggca ggcatatcgt ccgacatccc catagacagc
     1921 acgtctgcct taacgccagc cgcattgagg tcggcaagca gtttccgcat cgtttgaaat
     1981 tgcaccttca actccgtttc actgctgttg gctttggcaa cacacatcag tccacgtacg
     2041 acgatattcg gcagcttcgc cacttccaca gcaagcgcga ctgcttcttc gggcgcgaca
     2101 ccgtgcttca ccgcctcgcc cgcaatgttc acctcgatac acacctgcaa aggcggcatt
     2161 gaggaaggac gttgcccgct cagccggacg gcggttttca gacggcatac ggtatgcacc
     2221 caatgcgcgc gttcggcgac aaacttggtt ttgttggact gcacatctcc gatgacgtgc
     2281 cacacgatgt cggtcaaatc cgccaactct tccgttttgc cgtaccactc ctgaatatag
     2341 ttctcgccga aatcacgctg tccggcggcg taaacttcgc ggatgccgtc tgaagggaaa
     2401 gtcttaccga cggcaatcag gctgacggaa tgcggctccc tgcccgcctg cagaaccaat
     2461 tttccgatac ggtcggacac ctcacaataa cgttcttgca acaccgtcat agattatccc
     2521 ctaattaaaa atgattaaac agttgaaacc cctccagtca gggcggtaca atcaaggttg
     2581 ttagaaccat tccaaccaat cgaaacatta tactaaacag agccgcatta tgcagattac
     2641 cgacttactc gccttcggcg ctaaaaacaa agcatccgac cttcacctga gttcgggcat
     2701 atcccctatg attcgggttc acggcgatat gcggcgcatc aaccttcccg aaatgagcgc
     2761 ggaagaggtc ggtaatatgg taacttcggt gatgaacgac caccagcgga aaatctacca
     2821 gcaaaacttg gaagtcgact tctcgttcga actgcccaac gtcgcccgat tccgcgtcaa
     2881 cgccttcaac atcggccgcg gtcccgccgc cgtattccgc accattccca gcaccgtctt
     2941 atcgctggaa gaattgaaag ccccgagcat tttccaaaaa atcgcagaat cgccgcgcgg
     3001 catggttttg gttaccggcc ctaccggttc gggcaaatcg accacgcttg ccgcgatgat
     3061 caactacatc aacgaaaccc agccggcaca catcctgacc atcgaagacc cgattgaatt
     3121 cgtccaccaa agcaaaaaat ccctgattaa ccagcgcgag ctgcaccagc acaccctcag
     3181 cttcgccaac gcgctgcgtt ccgcattgcg cgaagacccc gacgttatcc ttgtcggcga
     3241 gatgcgcgac ccagaaacca tcggcttggc actgaccgcc gccgaaaccg gacacttggt
     3301 tttcggcacg ctgcacacga ccggcgcagc aaaaaccgtc gaccgtattg tggacgtatt
     3361 cccggcggga gaaaaagaaa tggtgcgctc tatgctgtcc gaatcgctga ccgccgtcat
     3421 ctcccaaaac ctgctgaaaa cgcacgacgg caacggccgt gtcgcctcgc acgaaatcct
     3481 gattgccaac cccgccgtcc gcaacctcat ccgcgaaaac aaaatcacgc agattaactc
     3541 cgtcctgcaa accgggcagg cgagcggtat gcagacaatg gaccaatcgc tgcaatcgct
     3601 ggtgcgccaa gggctgatcg caccggaagt cgcacgcaga cgcgcgcaaa acagcgaaag
     3661 tatgagtttc tgacacacaa ccgctttccg gccataccgg cgggaaaaca aggcgcaaac
     3721 acgcggggcg ggacgcagca tcccgcccgg ctacctttcc gaacaagaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aatggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cgcacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggcgctga cgggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccagtcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ccacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcaggaattg ggcctgcccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgccca gccatatcgt taccatcgaa gacccgattg aatttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc ccccgacgtg gtctgcatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aaccaaatac tgatcgacat cgccctcaac ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaacaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaagact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggatcaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aacgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgct acactttata gtggattaaa
     5161 tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag cacaaatagt
     5221 acggaaccga ttcacttggt gcttgagcac cttagagaat cgttctcttt cagctaaggc
     5281 gaggcaacgc cgtactggtt tttgttaatc cactatattc acttcataac aataaaccgg
     5341 taaaaccatg aaaaccccac tcctcaagcc tctgctcatt acctcgcttc ccgttttcgc
     5401 cagtgttttt accgccgcct ccatcgtctg gcagctaggc gaacccaagc tcgccatgcc
     5461 cttcgtactc ggcatcatcg ccggcggcct tgtcgatttg gacaaccgcc tgaccggacg
     5521 gctgaaaaac atcatcacca ccgtcgccct gttcaccctc tcctcgctca cggcacaaag
     5581 caccctcggc acagggctgc ccttcatcct cgccatgacc ctgatgacct tcggcttcac
     5641 cattttaggc gcggtcgggc tcaaataccg caccttcgcc ttcggtgcac tcgccgtcgc
     5701 cacctacacc acacttacct acacccccga aacctactgg ctgaccaacc ccttcatgat
     5761 tttatgcggc accgtactgt acagcaccgc catcctcctg ttccaaatcg tcctgcccca
     5821 ccgccccgtc caagaaagcg tcgccaacgc ctacgacgca ctcggcggct acctcgaagc
     5881 caaagccgac ttcttcgacc ccgatgaggc agcctggata ggcaaccgcc acatcgacct
     5941 cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa tgccgttccg ccctgtttta
     6001 ccgccttcgc ggcaaacacc gccacccgcg caccgccaaa atgctgcgtt actactttgc
     6061 cgcccaagac atacacgaac gcatcagctc cgcccacgtc gattatcagg aaatgtccga
     6121 aaaattcaaa aacaccgaca tcatcttccg catccaccgc ctgctcgaaa tgcagggaca
     6181 agcctgccgc aacaccgccc aagccctgcg cgcaagcaaa gactacgttt acagcaaacg
     6241 cctcggccgc gccatcgaag gctgccgcca atcgctgcgc ctcctttcag acagcaacga
     6301 cagtcccgac atccgccacc tgcgccgcct tctcgacaac ctcggcagcg tcgaccagca
     6361 gttccgccaa ctccagcaca acggcctgca ggcagaaaac gaccgcatgg gcgacacccg
     6421 catcgccgcc ctcgaaacca gcagcctcaa aaacacctgg caggcaatcc gtccgcagct
     6481 aaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg tccctcgtcg ttgccgccgc
     6541 ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac tggatactac tgaccgccct
     6601 tttcgtctgc caacccaact acaccgccac caaaagccgc gtccgccagc gcatcgccgg
     6661 caccgtactc ggcgtaatcg tcggctcgct cgtcccctac ttcaccccgt ctgtcgaaac
     6721 caaactctgg attgtcatcg ccagtaccac cctctttttc atgacccgca cctacaaata
     6781 cagtttctcc accttcttca ttaccattca agccctgacc agcctctccc tcgcaggttt
     6841 ggacgtatac gccgccatgc ccgtacgcat catcgacacc attatcggcg catcccttgc
     6901 ctgggcggca gtcagctacc tgtggccaga ctggaaatac ctcacgctcg aacgcaccgc
     6961 cgcccttgcc gtatgcagca acggtgccta tctcgaaaaa atcaccgaac gcctcaaaag
     7021 cggcgaaacc ggcgacgacg tcgaataccg cgccacccgc cgccgcgccc acgaacacac
     7081 cgccgccctc agcagcaccc tttccgacat gagcagcgaa cccgcaaaat tcgccgacag
     7141 cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc ctgaccggct acatctccgc
     7201 cctcggcgca taccgcagcg aaatgcacga agaatgcagc cccgacttta ccgcacagtt
     7261 ccacctcgcc gccgaacaca ccgcccacat cttccaacac ctgcccgaaa ccgaacccga
     7321 cgactttcag acagcactgg atacactgcg cggcgaactc gacaccctcc gcacccacag
     7381 cagcggaaca caaagccaca tcctcctcca acagctccaa ctcatcgccc gacagctcga
     7441 accctactac cgcgcctacc gccaaattcc gcacaggcag ccccaaaatg cagcctgaaa
     7501 aagtttcggc attttgtaag agaggacaga ttgtcagaca ggttacaaga tagtggataa
     7561 agctttgcgc agggtaaatg cgtagcaact gaaccgtcat tcccacgaac ctacatcccg
     7621 tcattcccac gaaaacagaa aaccaaaaac agaaacctaa aatcccgtca tttccacgac
     7681 a
//
LOCUS       DAWISC010000095         7660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWISC010000095 DAWISC010000000
VERSION     DAWISC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.095"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            318..452
                     /locus_tag="WIW87_001385"
                     /pseudo
     CDS             318..452
                     /locus_tag="WIW87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            407..622
                     /locus_tag="WIW87_001386"
     CDS             407..622
                     /locus_tag="WIW87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ5174539.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            825..1633
                     /locus_tag="WIW87_001387"
                     /pseudo
     CDS             825..1633
                     /locus_tag="WIW87_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1877..2533
                     /locus_tag="WIW87_001388"
     CDS             1877..2533
                     /locus_tag="WIW87_001388"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ5174540.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2689..4374
                     /gene="rpsA"
                     /locus_tag="WIW87_001389"
     CDS             2689..4374
                     /gene="rpsA"
                     /locus_tag="WIW87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ5174541.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4385..4699
                     /locus_tag="WIW87_001390"
     CDS             4385..4699
                     /locus_tag="WIW87_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ5174542.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5050..5457)
                     /locus_tag="WIW87_001391"
     CDS             complement(5050..5457)
                     /locus_tag="WIW87_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ5174543.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5605..6714
                     /locus_tag="WIW87_001392"
     CDS             5605..6714
                     /locus_tag="WIW87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ5174544.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            6723..7550
                     /gene="fghA"
                     /locus_tag="WIW87_001393"
     CDS             6723..7550
                     /gene="fghA"
                     /locus_tag="WIW87_001393"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ5174545.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2043 a         1866 c         1837 g         1914 t
ORIGIN      
        1 aattccctct ctttcatcat tatttaaatg ctatcattaa tataattaac aaaatttcat
       61 ttccatacac cgatgtagaa gaataacaaa ttaatctatt tataaactcc gattcttcct
      121 catcgggtta tactcatcag aaatactcac tgaaatatat gccttttaca tggaaaatca
      181 ctagacagtt gaaaagcaat cagtcagttc ctgttaaaaa acgcgtgtag aatttccgct
      241 ttatctcaat cggacacggt ttcaatatgt cttccccttc aaataccaat cgtcaaacct
      301 ggtccagccg attaacctat atcctgaccg ttgccggcgc gactgtcggt ttcggcgcga
      361 cgtggcgttt cccgtatttg gtcggtgaaa acggtggtgc gcgtatgtgt ttttattctg
      421 tatcgcgatg ctggttatcg gcatcccgat gattttggtg gaaaatgtca tcggacggcg
      481 caaaggcgtg aacgcgctgg atgcgttcgg cggcccgatg aacggcaaac ccattgccaa
      541 aatttggaaa ctggtcggct ggatgggctg ctcggcgcgt tcggcatcat ggcttattac
      601 atggtactcg gcggctgggt aatcagctat atcgttaata ttattggagg aaatttgaat
      661 atttccagcc ccgtcgacgg tgtggttaca aaaggcttct ttgccgaaca cattgaaaca
      721 gcccttggga aattgcgttt tatacgctgc tttttgtcgc cgtgaaccaa tggattttgg
      781 tcaaaggcgt tatcggcggc attgaaaaag cggcaaaata gctgatgccg ctgctgtttt
      841 tgttcctaat cgcgatggtc gtccgcaacg ttacccttcc gggcgcaatg gaaggggttg
      901 ctttctatct gaaacctgat ttcagcaaga ttaccgccga actgttcgtc ttcgttttgg
      961 ggcaggtatt ttttgccctg agcttgggtt tcggcgtgat gattaccttg tccagctatt
     1021 tggataaaaa cgaaaatctg gttcagacgg cagttatcac ggcaattacc aataccatca
     1081 tcgccatact tgcgggcttt atgattttcc cgtcgctctt cagcttcggc gttgcccccg
     1141 attccggccc gactttggtg ttccaaagct tgccgattgt gttctcacat atgtgggcgg
     1201 gatctgtgtt cgccgtgatt ttcttctcgc tgctcctgat tgccgcgttg acaacttcgc
     1261 tgaccattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
     1321 ccgcgattac gattgtattg gctgccatct tcattttcgg caacatcccg tccattctga
     1381 gctatggtcc gtggaaagac gttccgtgtt cggcaaaaat attttcgatg ccttcgacta
     1441 catcagcggc aacatcttgt ttatgctgac cgcgctcggt tccgcgctgt ttgccggttt
     1501 tgtgatgaag gacgaagcga aggacgaatt gctttataaa ggcaaccata cgacggtcaa
     1561 tatttggttt gcttatgtga aatatcttgt gccgctggtg attctgctga ttttcgtcag
     1621 caacctgttc taatccgcag caatcgatgc cgtctgaagg tcatacctct ttcagacagc
     1681 attgccttga tgcccgccac aatacccgca acgccgaaat ccgaaccggc ttgccggctt
     1741 ctgtcggatg tttccgggca ggcggcgttc cgtctgctta gacaatcgtc ctttaaaaca
     1801 ggtagaatcc gccccaacgg gaaacacatc cttcagacgg caaaacccat accccaaacc
     1861 atcaggaatc ccccttatga acagacaaaa agtcatcgcc atcgacggcc cgggcgcatc
     1921 gggcaaaggc acggttgccg cccgcgttgc cgccgcattg ggatacgatt atctcgatac
     1981 cggcgcactc taccgcctga ctgccctata tgcacaaaaa caaggcgtgg gatggcacga
     2041 tgaagaaaac gtttccgaac tggcaaaaaa actgcccgcc gtattttcag gcagccgcat
     2101 cctgctcggc ggcgaagacg tttcagacgg catccggaca gaagccatcg gcatgggcgc
     2161 atccgcagtc gcacagttgc ctaaagtccg cgccgccctg ctgcaacgcc aacgcgattt
     2221 tctgaccgaa aaaggactgg ttgccgacgg acgggacacc ggatcggtcg tcttccccca
     2281 agccgaactt aaaatcttcc tgacggcaga atccaaaatc cgtgccgaac gccgcgccaa
     2341 acaaatcggc atcccctgcg aaggtttggc attcgagcgc atcctgtccg acatcgaagc
     2401 cagagacgag gcagaccgaa accgcaaagt tgcccccctg aaacaacagc ccgatgccct
     2461 gcttttggac acaagccgcc tgactataga agaaactgta aaaaaagtgc ttgattggta
     2521 tcgtgaagtt taaattttca ggtataatcg cacaaattac gtttcagacg gcataaaaat
     2581 cccccatatg ccgtctgaaa ccttatgtac ccgtctgccc tgccaaggac ggcagatatc
     2641 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
     2701 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
     2761 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
     2821 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
     2881 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
     2941 ctgtcccgcg aaaaagccaa acgtgcagcc gattggattg ccctggaaga agccatggaa
     3001 aacggcgaca tcctgtccgg catcatcaac ggaaaagtca aaggcggcct gaccgttatg
     3061 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
     3121 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
     3181 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
     3241 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
     3301 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
     3361 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
     3421 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
     3481 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
     3541 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
     3601 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
     3661 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
     3721 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
     3781 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
     3841 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
     3901 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
     3961 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
     4021 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgttgacgcc
     4081 aaaggtgctg ttatcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
     4141 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
     4201 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
     4261 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
     4321 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
     4381 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
     4441 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
     4501 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
     4561 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
     4621 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
     4681 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
     4741 gtggtttaac ttcatccgtt gcttcaatac cttgagccaa gcaagcaacg gattagagcg
     4801 tggatttttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     4861 gagaacaatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttacactg
     4921 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatcatt gcttacaatc
     4981 cgctttttaa acaacaaatt tttgatttct attacgaaca ggacaaaaat cctgcttatt
     5041 gcactaaaac taagccgttt caggaatttg cggcaaattt acagctttta ccgagcctaa
     5101 tgctttcgct ttttggtaaa acgccaattt gtattcaagc aaatctaaat agcgttttaa
     5161 ttcggcaatt tgacacttca cattttctat ttgattttca aacaaggaaa ggcgttcttc
     5221 aatggtatcg tcgccaatga cggtacattc cgcaaagcgt ttgatgtctt ttaagctcat
     5281 tcccgtattt ttcaagcatt gcaataagcc caaccattgc aaatcgttat cggtaaaaca
     5341 gcggttaccg tattcatcac gtccgatatt gggcaacaaa ccttctttgt cgtaaaaacg
     5401 tagggtgtgg gcggagatgc ctattttttc ggcggctttg gcagtagtat aagtcatttt
     5461 ccctccttct aaacaaaaac agtaaaaaac acttgcttta gagttaactc taaagtgtaa
     5521 actgttgcta tgttgctcag gcaaggcaac tttgtcaatg aattaagagg aaagacaatg
     5581 gaaatgaaac aagccgattc aaccatcaaa tctcgtgcgg cggtggcatt cgcccctaac
     5641 caacccttac aaattgtgga aatcgacgta gaaatgccgc gtaaaggcga ggtgttaatc
     5701 cgcaataccc acactggcgt gtgccatact gatgcgttta cgttatcagg aagcgatcct
     5761 gaaggcgtat tccctgtggt gcttggacac gaaggtgcgg gtgtggtcgt tgctgtgggc
     5821 gaaggtgtgt caagcgtaaa accgggtgat cacgtgattc cgctttacac cgccgaatgt
     5881 ggcgaatgtg agttttgttg ttcaggtaaa accaacttgt gcgtctcagt gcgtgataca
     5941 caaggtaaag gcttaatgcc ggacggcacg acgcgttttt cttatcaagg tcagccaatc
     6001 tatcactata tgggctgttc gactttcagt gaatactccg ttgttgccga agtttcactg
     6061 gcgaaaatca accctgaagc caaccatgaa caagtatgtt tgctcggctg cggcgttacc
     6121 acaggtattg gtgcggtaca taatacggca aaagtgcaag aaggcgactc tgttgccgtg
     6181 tttggtttgg gggcgattgg tttggcggtg gtgcaaggtg cgcgtcaagc caaagccggc
     6241 cgcattatcg ccattgatac caatccatca aaatttgagt tggcaaaaca gttcggtgca
     6301 acggattgtt tgaacccgaa cgattacgat aaaccgatca aagatgtgtt gttagacatt
     6361 aataaatggg gcattgacca tacctttgaa tgtatcggca atgtaaacgt aatgcgtcag
     6421 gcattagaaa gtgcacatcg tggttggggt caatccatta tcatcggcgt agcaggtgca
     6481 ggacaagaaa tttcaacgcg tccgttccag ttggtaacag gtcgtgtttg gaaaggttca
     6541 gcatttggcg gtgtgaaagg tcgctctgaa ctgccgaaaa tggtggaaga ttcaatgaaa
     6601 ggcgacattg agttagaacc gtttgtaacc cacacaatga cactcgatca aatcaataaa
     6661 gcctttgact taatgcacga aggtaaatcg atccgcgccg ttattcacta ctaaggtatg
     6721 cgatgaaact gattgaacaa catcaaattt ttggtggttc gcaacaagtt tgggcgcatc
     6781 atgcccaaac gctgcaatgc gaaatgaaat ttgccgtcta tttgccaaat aatccagaaa
     6841 atcgaccgct tggtgtgatt tattggcttt ccggcttgac gtgtacggaa caaaatttca
     6901 ttaccaaatc aggctttcag cgttatgcgg cagaacatca agtaattgtg gtggcccccg
     6961 ataccagccc tcgcggagag caagtgccga acgatgatgc ttacgattta ggacagagtg
     7021 caggctttta tttgaatgcg accgaacagc cttgggcggc gaattatcaa atgtatgatt
     7081 acattttgaa cgagctgccc cgtctgattg agaaacactt tcctaccaac ggcaaacgtt
     7141 ccattatggg acattcaatg ggcggacacg gcgcattggt attggcgctg cggaatcagg
     7201 aacgttatca aagtgtttct gccttttcgc ctattttatc gccaagcctc gtgccgtggg
     7261 gagaaaaagc ctttactgct tatttaggga aagaccgtga aaaatggcag caatatgatg
     7321 ctaactcact cattcaacaa ggctataaag tgcaaggtat gcgcatcgat caaggcttgg
     7381 aagatgagtt tttgccgaca caattgcgta ccgaagattt tatcgaaacc tgccgtgcgg
     7441 caaaccagcc ggtcgatgtg cgtttccata aaggctacga tcacagctat tacttcatcg
     7501 ccagttttat tggcgagcat attgcttatc acgccgcgtt tttgaagtaa accaaagagc
     7561 gttcagtgtt caaagcagtt ttgggatagc cggcacgagg gcggtaagaa gtgccggcat
     7621 aaacgtatgc cgtctgagcc gaaaggagcc gactctacgg
//
LOCUS       DAWISC010000096         7648 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWISC010000096 DAWISC010000000
VERSION     DAWISC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.096"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WIW87_001394"
     CDS             complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WIW87_001394"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ5174546.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1315..2004)
                     /locus_tag="WIW87_001395"
     CDS             complement(1315..2004)
                     /locus_tag="WIW87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ5174547.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2201..2980
                     /gene="rsmA"
                     /locus_tag="WIW87_001396"
     CDS             2201..2980
                     /gene="rsmA"
                     /locus_tag="WIW87_001396"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ5174548.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKVV"
     gene            3153..3881
                     /locus_tag="WIW87_001397"
     CDS             3153..3881
                     /locus_tag="WIW87_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174549.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3954..4313
                     /locus_tag="WIW87_001398"
     CDS             3954..4313
                     /locus_tag="WIW87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174550.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     NMEEAELNITPVFDNMM"
     gene            4318..4764
                     /locus_tag="WIW87_001399"
     CDS             4318..4764
                     /locus_tag="WIW87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ5174551.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRQCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            4795..6069
                     /gene="folC"
                     /locus_tag="WIW87_001400"
     CDS             4795..6069
                     /gene="folC"
                     /locus_tag="WIW87_001400"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ5174552.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKIGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6082..7080
                     /gene="ftsN"
                     /locus_tag="WIW87_001401"
     CDS             6082..7080
                     /gene="ftsN"
                     /locus_tag="WIW87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ5174553.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7073..7570
                     /locus_tag="WIW87_001402"
     CDS             7073..7570
                     /locus_tag="WIW87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ5174554.1"
                     /translation="MNSLPIADLLVSAVIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTSAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1931 a         2165 c         1974 g         1578 t
ORIGIN      
        1 tgttaatcca ctatatcaga cggacgaggt tacagtttaa tccctttgag cttcgcgacg
       61 gtattgatgt ctttgtcgcc acgacctgag aggttgacca aaatcacttg gtctttaccc
      121 attttcggcg catttttcac cgcccaagca acggcgtggc tggattcgag cgcggggatg
      181 atgccctcga agcggcagag caagtcaaag gcttcgagtg cttcgtcgtc tttggcaaca
      241 gtgtattcga cgcgcttgat gtcgtgcaga tggctgtgtt ccgggccgat gccggggtaa
      301 tccaagcctg cggaaacaga gtgcgtaccc aaaacctgac cgttttcgtc ctgcatcaga
      361 tagctgcgga aaccgtgcaa tacgccaatc ggtgcgcccg aagtaatcgg cgcggcgtga
      421 tcgggggtgt tcacgcccaa accgccagcc tccacgccga cgaggcgcac gttttcttcg
      481 ccgatatacg ggtggaacaa accgatggcg ttcgatccgc cgcccacgca ggcaacggca
      541 acgtcgggct gtctgccgat ggcttcctgc atctgcgctt tagcttcgtt gccaatcacg
      601 cattggaaat cgcgcaccat ttcgggatac ggcgcggggc cggcggcggt accgatgatg
      661 tagaacgtgt cgtccacgcg ggcgacccat tcgcgcatgg cttcgttcat cgcgtctttc
      721 agcgtgcggc tgccgctttc tacaccgacc acgttcgcac ccaataattt catacggaac
      781 acgttgggca tttggcgttg gatgtcgtcc gcgcccatat acacgtcgca agtcataccg
      841 aagcgtgcgg caacggtggc actcgccacg ccgtgctgac ccgcgccggt ttcggcgatg
      901 acgcgttttt tacccatgcg ttttgccaac agtgcctgac cgatggtgtt gttgactttg
      961 tgcgcgccgg tgtggttcaa gtcttcgcgc ttcaaccaga tttgcgcgcc gcccagatgt
     1021 tcggacaacc gcgcggcgtg gtaaacgggg ctgggcctgc cgacataatg tttcaaatca
     1081 tggcggaacg cttcccaaaa ttcaggatcg tttttcgctg ccttataggc atccgccagc
     1141 tcttgcaagg cgggaatcag ggtttcggag acataaagcc cgccgtgttc gccgaaaaag
     1201 cccttctcgt cgggcgcgtg gtagttcttc atcggattct tccttgtgct tcaaattttc
     1261 agacggcatt tccgtttttg caaacatgcc gtctgaacgg gaaattgtcg gcaattataa
     1321 ctgtttttca cacaagcccg ccatttcaaa aaacgcccga tacgccgccg cttttttcgc
     1381 caaactcaaa ggataggcgc gcgaagtcga aggcaggcgc gtcagcgtca aatcccgtcc
     1441 ggcaaacgga aacggcacgg tttcgcccgt tttcggcatt ttgataccgc cgccctgaat
     1501 atcgagcagg atttccgtcg ccttgccgcc tgtcgtacaa atatggcggc actcgggtat
     1561 ttttgccaac accgccgcca aatcgacggt ttcaactacc tttaaaaact tgtcggacgc
     1621 attgccgtgc tgacgtaccg ccttcaaaac ggtcggacag gacgcaatcc cccgttcgtg
     1681 caaaaacgcc ttgattttct cggcatcaaa cgctttttca gacaaccttt ggaaatgcgc
     1741 cgcatcatta aaaaacacca gcccataaac gcgccacata tcgttttgga aattcggata
     1801 atgaaactgc atcgcgcgtt tgtcttcctt gggcggaaac gtccccatca tcatgacagc
     1861 agccttcggc ggcaacacgg gatcgaaggg atgggtttcg atttcaagca gattttcaga
     1921 cacgttcaat cctccaacag gcaaaacggc agacagccgg tttttgcacc aaaccgccaa
     1981 acacggcaag ccttcaaaca gcattatcac gccctgccgt tttcggttac aatgccgaca
     2041 tcaaacggat actgtaaaca catttatgtt tcaacacaca ggacgacaca taaagcaccg
     2101 ccctatgtgc catcctgatt tggaaagagt tacgcccctc ccaaatagag tctgattcta
     2161 ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagaac acaaagcccg
     2221 caagcgtttc gggcagaatt ttttgcagga cacgcggatt atcagcgata ttgtcaacgc
     2281 cgtgcgcccg caggcggatg atgtcgtgat tgaaatcggc ccgggtttgg cggcgattac
     2341 cgaacctttg gcgaaaaagc tgaaccgcct gcacgttgtc gaaatcgacc gcgacatcgt
     2401 atgccgtctg aaaacgctgc cgtttgcgga taaactggtg attcacgaag gcgatgtatt
     2461 gcagtttgat ttcaacggca tcgcaggcaa aaagaaaatc gtcggcaacc tcccgtacaa
     2521 catttccaca ccgcttttgt tcaagctggc ggaggtggcg gacgatgtcg tcgatatgca
     2581 ctttatgctg caaaaagaag tggtcgagcg tatggttgcc gcgccgaaaa gcaacgacta
     2641 cggccgcttg ggcgtgatgc tgcaatattt tttcgatatg gaaatgctga ttgacgtacc
     2701 gcccgaatcg ttcgaccctg cgccgaaagt ggattccgcc gtcgtccgta tgattccggt
     2761 gaaacaccgc atcggcaagg cggacgattt cgagcatttt gccaaactcg tgaaactcgc
     2821 cttccaccaa cgccgcaaaa ccatacgcaa caatctgaaa gagcttgcag gcgacgatga
     2881 tttgcaggca gtcggcatca atccgcaaga ccgcgccgaa catatcgcgc cagagaaata
     2941 cgtggcgttg agcaattatc tggcgggcaa ggtcgtctga aaagggagta agccgatgac
     3001 gaaatacctg cttttacccc tgatactgcc gcttgcagcc tgcctgacca atccgaacga
     3061 ccgttttttt gacggcagac gctacacgct tcatcatatg gagctcccgt aacgacaacc
     3121 tgcaacggca aatgccgtct gaaagaaaag acatgatcaa attcaaaaac gtacacaaac
     3181 atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa ggcgaagtgg
     3241 tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcact gtcaaccagc
     3301 ttgaaagcat cgaaagcggc gagatttggg tggacggtgt caacgttgcc gaccccaaaa
     3361 ccgatttgaa caaaatccgc gaagaagtcg gcttcgtatt ccaaggcttc aacctctacc
     3421 cgcacctgac cgtgttggaa aacattaccc tcgcaccgat gaaggttaag gggcaaaatg
     3481 cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg gcacacaaaa
     3541 aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg attgcacgcg
     3601 gtttggcgat ggaaccgcgc gtaatgttgt ttgacgaacc gacctccgca ctcgaccccg
     3661 aaatggtcgg cgaagtgttg aaagtgatga aagacttggc agaaagcggc atgacgatga
     3721 tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg attttcgtcg
     3781 ataaagggca aatcctcgaa gacgaaacgc ccgaagcgtt ttttaccaac ccgaaacacg
     3841 aacgcgccaa gcagttcctg caacaggtca tgacccactg atgctatacc gtctgaaaac
     3901 cgaacgttca gacagcatat cccgacaccg caagatgatt gagaaagaaa accatgtatt
     3961 ttgttgaccg caccgccgtc gtcctcaagc cgaccgcgcg ctttttggaa tggctcaaaa
     4021 gcaccgacga aaatatgccc gacctgacca tagagcagct tcgtgccaac tgttccgtct
     4081 tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac gaacgctacc
     4141 ggcagatttt cgaggcggag ctggcaggtt gggacatcga taaggataaa tggccgcaag
     4201 atatggggct gaaggcgttt tgggaatttt tcgacatcga aatccacgat atggtgttga
     4261 acatggaaga agcggaattg aacattaccc ccgtgttcga caacatgatg taaggctgtg
     4321 cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac tgtatcgctt
     4381 cacgccgcct cccttgccgt ctgcctgact tggttttacg gccggatgat gtggttcggt
     4441 ttggcggctt tggtggcaag ttacgcatac tcgctcagga taacgaatct gaaacaccgc
     4501 catgccataa ccgccattac catagaccgc gacggacagg cggaaatcgt atccggcaaa
     4561 gacaaaacgg caaaggcggc ggcactgtcg ggcagcagta tggttacgcc ttatgcctta
     4621 ttcttacaat gggacaccgg cggcaaaacc gtccgccaat gcatcatgcc cgacatgacg
     4681 gacaaagaat cctaccgcaa gctgaaagtt tgggtacttt ggcggcagcc gaaaaaaacc
     4741 gccgaaaccg atacatcaga ctgatttcag acggcatttg aaaacgaaac actcatgaaa
     4801 acactacaag actggctctc gcatttggaa accgcccaca gcggcggttt gatcgacatg
     4861 ggtttggagc gcacgagcga agtgaaaaaa cgcatgaagc tcgagccgca atgccccgtc
     4921 gtcgttgtcg cgggaaccaa cggcaaaggt tcggtctgtg cctacctgac acagatttac
     4981 aaacaggcgg gctacaaaat cggtacgctg accagcccgc atttattgcg ttacaacgaa
     5041 cgcatcgcca tcaacgccga acccgtttcg gacgatacga ttattgcctc tttcgagcgc
     5101 atcgaagccg cgcgcggcga aatatcactg acttattttg aattcaatgc cttggcggct
     5161 gtcgacatct tcatgcgcga acaggttgac gtgatgatat tggaagtcgg cttgggcggc
     5221 cgcttggacg cggtcaacgc gtttgacggc gattgcgcgg tggttaccag cgtggatttg
     5281 gaccatcagg catttttggg cgatacggtc gagcaggtcg gctttgaaaa agcaggcgtg
     5341 ttccgtagcg gcaaacccgc catctgcggt caaaaccccg cgcccaaatc gttgctttca
     5401 cacgccgaag ccataggcgc gaaactgctg atggtgcagc gcgatttcga gtttcacgcg
     5461 atggaaaaca tccaatggaa ctaccgcttc cggccgcaac attcagacga ccccgcgcgc
     5521 aaccgcaatg ccctgccctt ccccgcattg cgcggcacat accagctttc caatgccgcc
     5581 tgcgcgctga ccgtattgga atgcttgaac gacaaattgc cggtggacat cggtgccatc
     5641 aagcgcggtt tgttgctggt tgaaaatccc ggacgcttcc aagtcctgcc cggccggccg
     5701 ctgaccgttt tggatgtcgg acacaatccc cacgccgccc gcgccctgcg ccgcagcctg
     5761 attaatttgg cgtttgcgca aaaacgcacc gccgtgttca gcatgttgtc cgacaaagac
     5821 atagacggcg tgttggaaac cgttaaagac cagtttgacg agtggtatat cgcaccgttg
     5881 gatgtaccgc gcggcatgac gctcgagagc ctgcaaaaca aactgcacga acacagcatc
     5941 gaaaacatac aaacattcgc cgccgtccgc gaagcatacc gggcggcagc ctcaaaagcg
     6001 ggcgagaacg acagaatcgt cgttttcggc tcatttcata ccgttgccga tgtcatgtcg
     6061 gtactttaaa aggaaacttc tatgtccgaa aacaaacaaa acgaagtcct gagcggttac
     6121 gaacaactca aacggcgcaa ccgccgccgc ctcgtaacgg caagttgcct ggttgccgcc
     6181 tcctgcatcc tgctggcagc cgccctcagt tccggccctg ccgaacagac tgccggcgaa
     6241 acaagcggcg tagaaaacaa agcggcaggt gcggcacaaa cccctgcctt gaaatccgcc
     6301 gccgacaaac cgcaggactt ggcaggcgaa gacaagcctt ctgccgccga cagcgaaatc
     6361 agcgagcctg aaaacgtagg cgcgccgctg gtgctgatta acgagcgcct cgaagacagc
     6421 aacatcaaag gtttggaagc atccgagaaa ctgcaacagg cagaaaccgc caaaaccgca
     6481 ccgaagcagg caaaacaacg cgctgccgaa aaagtgccgg caactgccga cagtacggat
     6541 acggtagcgg ttgaaaaacc gaaacgcact gccgaaacaa aaccgcaaaa agcggaacgc
     6601 actgccaaag ccaagcccaa agccaaagaa accaaaaccg ccgaaaaagt tgccgacaaa
     6661 ccgaaaactg ccgccgaaaa aaccaaaccg gatacggcaa aatccgacag cgcggtaaaa
     6721 gaagcgaaaa aagccgacaa ggctgaaagc aaaaaaacag ccgaaaaaga ccgttcggac
     6781 ggcaaaaaac acgaaacggc acaaaaaacc gacaaagcgg acaagaccaa aaccgccgag
     6841 aaggaaaaat ccggtaaaaa agccgccatt caggcaggtt atgccgaaaa agaacgcgcc
     6901 ttaagcctcc agcgcaaaat gaaggcggcg ggtatcgatt cgaccatcac cgaaattatg
     6961 accgacaacg gcaaagttta ccgcgtcaaa tcaagcaact ataaaaacgc aagggatgcc
     7021 gaacgcgatt tgaacaaatt gcgcgtacac ggtatcgccg gtcaggtaac gaatgaatag
     7081 cctccccatt gccgacctcc tcgtctccgc cgtcatcgcc gcctgcatcg tgctatccgc
     7141 gatgcgcggc gtgattgcgg aggcaggctc aatggcggca tgggtggttt ccttcttttt
     7201 cgccaaactc tttgccgcct ccttcgccga cctcgccttt gcctcgttcc aaccccgcct
     7261 gtttgcattg gctctgtcgt tcatttccct gttcgtcatt gcctgtctga tccagaaaat
     7321 gctccgttcg ctgctgacca gcgcagtttc ggcggtcggt ttgggctttg ccaaccgcat
     7381 tttgggcggc gtattcggtg cattgaaagg cgttttgatt gttaccctgc tggtcatgct
     7441 tgcttcaaaa accgacctgc ccgataccga agaatggcgg caatcttaca cactgccgtt
     7501 tttcgtatcg ctttccgaag ccgtgttgaa ccatagcggc ggcacggcgg aaactccgga
     7561 agacgattga ttgccatacg gatatttcag acggcatcct cttttatgcc gtctgaaatg
     7621 ttttttcctt tcctcagcca tatcacag
//
LOCUS       DAWISC010000097         7619 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWISC010000097 DAWISC010000000
VERSION     DAWISC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.097"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(100..1017)
                     /locus_tag="WIW87_001403"
     CDS             complement(100..1017)
                     /locus_tag="WIW87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174555.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            1190..1990
                     /locus_tag="WIW87_001404"
     CDS             1190..1990
                     /locus_tag="WIW87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174556.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            2038..2814
                     /gene="mlaE"
                     /locus_tag="WIW87_001405"
     CDS             2038..2814
                     /gene="mlaE"
                     /locus_tag="WIW87_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ5174557.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            2865..3359
                     /gene="mlaD"
                     /locus_tag="WIW87_001406"
     CDS             2865..3359
                     /gene="mlaD"
                     /locus_tag="WIW87_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ5174558.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            3396..3986
                     /locus_tag="WIW87_001407"
     CDS             3396..3986
                     /locus_tag="WIW87_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ5174559.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            4037..4315
                     /locus_tag="WIW87_001408"
     CDS             4037..4315
                     /locus_tag="WIW87_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ5174560.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            4312..5139
                     /locus_tag="WIW87_001409"
     CDS             4312..5139
                     /locus_tag="WIW87_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ5174561.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            5264..5626
                     /locus_tag="WIW87_001410"
     CDS             5264..5626
                     /locus_tag="WIW87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ5174562.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            5623..6009
                     /locus_tag="WIW87_001411"
     CDS             5623..6009
                     /locus_tag="WIW87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ5174563.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            6006..6494
                     /locus_tag="WIW87_001412"
     CDS             6006..6494
                     /locus_tag="WIW87_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ5174564.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(6575..6742)
                     /locus_tag="WIW87_001413"
     CDS             complement(6575..6742)
                     /locus_tag="WIW87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ5174565.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(6709..7089)
                     /locus_tag="WIW87_001414"
     CDS             complement(6709..7089)
                     /locus_tag="WIW87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ5174566.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            7099..7225
                     /locus_tag="WIW87_001415"
                     /pseudo
     CDS             7099..7225
                     /locus_tag="WIW87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7327..7542
                     /gene="rpmE"
                     /locus_tag="WIW87_001416"
     CDS             7327..7542
                     /gene="rpmE"
                     /locus_tag="WIW87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ5174567.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         1785 a         2206 c         1930 g         1698 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
       61 actatacacg cggatttcgg gtttgaaatc aaatgcggct taccggtttt cttttttggt
      121 ttccgcaggc gtgctgccga aatattgcct gaatgcctga gtaaagctgg aaacgtggcg
      181 gtagccgcat aaatatgcgg tttcgcctat gctttttccc ccgttttgca acagatagag
      241 cgcgtgctgc atttgtttgt ggtgcagcca ttcgcttgcc gtaatgccga aatggtcgcg
      301 catacggcgt tgcagcgtcc tttcactgat gttcagcgcg tctgtcagcc ggttgacttg
      361 gtgtgcgccg tcggcaaacg cggcatttag ggtgcggctg aagtcttcag acggcatagc
      421 gtctgcttcc gccgtttgcc ccgccgccgg cccgatgccg tctgaaaccg tgtcccacaa
      481 gtccgacagc agccgcaaca cgtccgcctc gcggcgcaat gtttcgccca aatgcccctt
      541 tgggacggct ttcaggcagg atgccgccaa gccgcgcagg ttcgggggca aatcccatat
      601 cctgaccggt tcgcggtaaa gcaggggtgc gaaacgcgcg tattccggac gcagcagcca
      661 ttgttccata cctttaatgg tcattttgac cgttttgccg cctcggtaaa gatagcggct
      721 gaacaggact tcttccccga cagcaattag gacaatcttg ccgccgtccg catcgatttg
      781 gaagcggcag cggttgatgc cgaagtccaa actgccttcc agcaagagca cgaacgacac
      841 ataaggttcg gcgaggcggc tgctgcaaaa atcacaccgt gccgttactg tgccgccgtg
      901 gatggaaatg ccgttggaca aagtatcgaa acggtagctg ccctccacat agctgccgcc
      961 gccgcttttg actagattga cgaaccggct gctgttcaaa tgttctgctt tttccatagt
     1021 tttctgataa aggttatcat ttgaaagata acatttttcg ccacagcaaa caatccgccg
     1081 ccgacaacgg cattttcaca acatttaccg tccgtatgcc gtctgaaaat tgcccgtctt
     1141 cccgataagc cttaaaatac acgccgtcaa acctaccgtc cgtccgtaca tgagcccatc
     1201 cccctttatc gaaatgaaag acgtcgcctt cgcgtatggc gaccgcccga ttctgaagaa
     1261 catcaatttc agcattccgc aaggcaattt tgctgccgta atgggcggtt cgggcagcgg
     1321 caaaaccacg ctgatgcgac tgattacagg acagattcgt ccgcagtccg ggcaggtttt
     1381 gattgaagga cgggatttgg cgggcttttc ggctgacgag ttgtatgaac accgccgccg
     1441 tatgggcgta ttgttccaac acggcgcgct gtttactgat ttgtcggtat tcgacaatat
     1501 cgcttttccg atgcgcgaac tgacgcgcct gccggaagcg gtgattcggg atttggtttt
     1561 gttgaaattg aacgcggtcg gtctgcgcgg tgtggaaaac ctgatgccgt ccgagttgtc
     1621 cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacgatt gcgctcgacc ctgaaattat
     1681 gttgtacgac gagccgttta ccggcctcga tccgatttcc ttgggcgtga ttgcccactt
     1741 gatcagccgc gtcaacaagg ctttgcgttc gaccagtatt atggtaacgc acgacattga
     1801 aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg cacggcgaaa ttatgttctc
     1861 cggctcgccg caggaaatgc gcgaactgga ttcgccttgg gtgcgccagt ttgtcggcgg
     1921 gctggcagac ggccccgtag cataccgtta tccggcgcaa acgtcgttgc agcaggacct
     1981 gttgggataa atgagttgat gccgtctgaa accgttgaac tttacacata gaaatttatg
     2041 aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta ttcaatccct cggcagtatc
     2101 acgctgtttc tgctgaacat tctggcgaag tccggtacgg ctttcgtccg tccgcgcctg
     2161 agcgtgcgcc aagtgtattt cgccggtgtg ctgtcggtgt tgattgttgc cgtttcgggg
     2221 ctgtttgtcg gcatggtctt gggtttacag ggctatacgc agttgtcgaa attcaaatcc
     2281 gccgatattt tgggctatat ggtcgcggct tcgctattgc gcgagctggg tccggtattg
     2341 gcagcgattt tgtttgccag cagcgcgggc ggtgcgatga ccagcgaaat cggtctgatg
     2401 aaaacgaccg aacagctcga agcgatgaat gtgatggcgg taaaccctgt cgcccgcgtg
     2461 gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc ttttggcttc gattttcaac
     2521 gtggcgggca ttttcggtgc gtatttggtt ggtgtaacct ggctgggctt ggacagcggt
     2581 attttctggt cgcaaatgca gaacaacatc acgatacatt acgatgtaat caacggtctg
     2641 atcaaatccg ccgcgttcgg cgtggcggta acgctgattg ccgtgcatca gggcttccat
     2701 tgcgtcccga cttcggaagg cattttgcgc gccagcacgc gcacggtggt ttcgtccgcc
     2761 ctgacgattt tggcggtcga ttttatattg accgcgtgga tgtttacaga ttgacaggcc
     2821 gtctgaagac gaaacgcgga acatcggata ttcaaggaac tttaatgaaa aagaacatat
     2881 tggaattttg ggtcggactg ttcgtcctga ttggcgcggc ggcggttgcc tttctcgctt
     2941 tccgcgtggc cggcggtgcg gcgttcggcg gttcggacaa aacttacgcc gtttatgccg
     3001 atttcggcga catcggcggt ttgaaggtca atgcccccgt caaatccgca ggcgtattgg
     3061 tcgggcgcgt cggcgctatc ggacttgacc cgaaatccta tcaggcgagg gtgcgcctcg
     3121 atttggacgg caagtatcag ttcagcagcg acgtttccgc gcaaatcctg acttcgggac
     3181 ttttgggcga gcagtacatc gggctgcagc agggcggcga cacggaaaac cttgctgccg
     3241 gcgacaccat ctccgtaacc agttctgcaa tggttctgga aaaccttatc ggcaaattca
     3301 tgacgagttt tgccgagaaa aatgccgacg gcggcaatgc ggaaaaagcc gccgaataaa
     3361 cgtatgccat catattcaaa ctaggacact taatcatgaa aaaatcctcc ctcatcagcg
     3421 cattgggcat cggtattttg agcatcggca tggcatttgc cgcccctgcc gacgcggtaa
     3481 gccaaatccg tcaaaacgcc actcaagtat tgagcatctt aaaaaacggc gatgccaaca
     3541 ccgctcgcca aaaagccgaa gcctatgcga ttccctattt cgatttccaa cgtatgaccg
     3601 cattggcggt cggcaaccct tggcgcaccg cgtccgacgc gcaaaaacaa gcgttggcca
     3661 aagaatttca aaccctgctg atccgcacct attccggcac gatgctgaaa ttaaaaaacg
     3721 ccaacgtcaa cgtcaaagac aatcccatcg tcaataaagg cggcaaagaa atcatcgtcc
     3781 gcgccgaagt cggcgtaccc gggcaaaaac ccgtcaacat ggacttcacc acctaccaaa
     3841 gcggcggtaa ataccgtacc tacaacgtcg ccatcgaagg cgcgagcctg gttaccgtgt
     3901 accgcaacca attcggcgaa attatcaaag cgaaaggcgt ggacggactg attgccgagt
     3961 tgaaagccaa aaacggcggc aaataaccga caaaccatac cgtccgaggg cggtatgggt
     4021 tttccgagca aacgctatgc atacagaact caaaaacgga atattgcaca tcggcggcga
     4081 catcaccgtc aaaaccctga ccgccgccgc ttttacccgc ttcagacagc aatgtcgtct
     4141 gaaagacacc tgcgcggttg atttgagcgg cgtcggacgc gccgattccg cctgcgtgtc
     4201 gctgctgctc gaagtgctgc gcggttgcaa aggcagcgtc aggctgaccg gcattcccga
     4261 atccgtgcgc gcgctgtccg aactgtacga aatcaaagac tggctgaaat catgaaaaaa
     4321 accgcctatg ccttcctcct gctgatcggg ttcgcttccg cccctgcatt tgccgaaacc
     4381 cgccccgccg acccttatga aggctacaac cgcgccgttt tcaaattcaa cgaccaagcc
     4441 gaccgctaca ttttcgcccc tgccgcgcgc ggctaccgca aagttgcgcc gaaacccgtc
     4501 cgcgccggcg tgtccaattt ttttaacaac ctgtgcgacg tggtcagctt cggcagcaat
     4561 atcttgcgct tggacatcaa acgcgcaagc gaagaccttg tccgcgtcgg catcaacacc
     4621 actttcggtt tgggcgggct tatcgacatc gccggcgcgg gcggcattcc cgacaataaa
     4681 aacaccttgg gcgacacgtt tgcctcgtgg ggatggaaaa acagcaatta tttcgtgttg
     4741 cccgtcttag ggccgtccac cgtccgcgac gcgctcggca cgggtattac ctccgtttat
     4801 tcgcccaaga atatcgtctt ccgcacccct gtcggacgct ggggcacgac tgccgtatcc
     4861 gccgtcagta cgcgcgaagg cctgctcgat ttgaccgaca gtctggacga agccgccatc
     4921 gacaaataca gctacacgcg cgacctctat atgaaagtcc gtgcgcggca gaccggtgca
     4981 acacctgccg aaggtacgga agataacatc gacatcgacg aattggtcga aagtgccgaa
     5041 accggcgcgg cggaaactgc cgttcaagaa gattccgtat ccgaaacaca ggcagaagca
     5101 gcaggggaag ccgaaacgca acctggaaca caaccttaaa gccatgccgt ctgaaaatcc
     5161 gtgttcggac ggcattccct tctgtcggaa cggacagtcc gaaaccacag attgggctat
     5221 aatctctttt ttgcgcatac cgcgcgacag caaaggaaaa accatgtacg aagtcaaccg
     5281 cagcgtattc gtcctcatcc ctttagaacc tttttggaac tggctgcaaa ccctgccggg
     5341 caaccacctc gacggactga ctcttgaaga catccaagcc gacgccaatt cctaccttgt
     5401 ccgcccgtgc gaaaccgccg acgaagtatg ggacgaaatc gaagcccgtt ttgaagacat
     5461 tttcgccgcc gaacttgccg actggtgcga agacgaacgc gagtggcctg cgctcgatgc
     5521 cgacattttc aacgaatggt tcgacatcca gctttctacc gtcatcaccg acctcgaaca
     5581 cgaaccgctt gcccgcgaag ccttccaacc catcaacctg aactgatgaa gctgaccgtc
     5641 cgcaactacc atctcgacgg ctacggccat gtcaacaatg cgcgctacct cgaatttttc
     5701 gaagaagcgc gctgggcgtt ttttgaagaa cgcggactgc tgcacgagtt ggcaggcctc
     5761 atactgattg tcgcccgcat cgacatccga tacagccgcc ccgccgtcga aggcgacgta
     5821 ttgcagtttt catgccgcct gaaaaccccc ggcacgcgcc gtattgtgct gacccagacg
     5881 attacactgc caaacggcaa aaccgccgca gaagccgaca tcaccctgat gcccgtccac
     5941 gccgccacac aacgcaccgt cagcctgccc gccacccttg cccgcgcatt ggaagccttg
     6001 tccgaatgaa aaaaatactc accgccgccg tcgtcgcact gatcggcatc ctccttgcca
     6061 tcgtcctcat ccccgacagc aaaaccgcgc ccgccttctc cctgcccgac ctgcacggaa
     6121 aaaccgtttc caacgccgac ctgcaaggca aagtaaccct gattaatttt tggtttccct
     6181 cctgtccggg ttgtgtgagc gaaatgccca aaatcattaa aacggcaaat gactataaaa
     6241 acaaaaactt ccaagtactt gccgtcgccc agcccatcga tccgatagaa agcgtccgcc
     6301 aatatgtcaa agactacggt ttgccgttta ccgtcatgta tgatgcggac aaagctgtcg
     6361 gacaggcgtt cggcacacag gtttatccga cttccgtcct tatcggcaaa aaaggcgaaa
     6421 tcctcaaaac ctacgtcggc gaacccgatt tcggcaaact ctaccaagaa atcgataccg
     6481 cgctggcgca atagcgatgc cgtctgaagc gcaaaaaaca gaaacgatgt tcctgttttt
     6541 tcagatcatc cgccccgaca cgttcagacg gcattcaacc ttccggcaga ttctttttca
     6601 ccactgccgg attgcctgcc gccaaagagt agggcggaat gtcttttgta accaccgcgc
     6661 ctgcgcccac gaccgaaccg cgtccgacgg ttacgcccgc cattacaatc accctgtgcc
     6721 ccggccagac atcgtcctcc aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt
     6781 ttttgaacgg tcaaacttgt ggttatttga ataaaacaga cattccggct ccatcatcac
     6841 atttttgccg accaccagcc cacggcagat ttcacagttt gccccgatgc ccgagccgtc
     6901 gcccaaaacc gtatccggaa acacatacgc cccgcgttcg atattgaccc cgcgtccgat
     6961 atgcggagaa acccgccgcg ccaaaaaacc gcgcacccgt ctgccgacga tgccgatgcc
     7021 gcgcatatgt gacggcggca accccgaacc ggcaaaacgc agcagcagga aaaagaattt
     7081 tgatcccatc aaacctccct caaaagaact ttttatagtg gattaacaaa aattaggaca
     7141 aggcaacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg ctgtactggt
     7201 ttttgttaat ccactataca ttttatcgaa tatactcttg acgtaacaac agaaaatagc
     7261 gagaatgtcg gacttgtctg tagcataggt acatccccta tgcccgcttt tttgaaagga
     7321 aatcaaatga aacaaggtat tcacccgaac taccacgaag tcaacgttac ctgctcttgc
     7381 ggcaacaaat tcgcaaccaa atccgcgatg gaaaaagaaa acttcaatat cgaggtttgc
     7441 tccctgtgcc acccgttcta taccggcacc caaaaaatcg ttgataccac cggccgcgtg
     7501 gacaaattca acaacaaatt cggcaacctg ttcaaacgct gattgccgct ttgcaagaat
     7561 taagccctgc catcatttcg atggcagggc tttttaatat ttggcacact gacttctta
//
LOCUS       DAWISC010000098         7585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWISC010000098 DAWISC010000000
VERSION     DAWISC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.098"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(53..361)
                     /locus_tag="WIW87_001417"
     CDS             complement(53..361)
                     /locus_tag="WIW87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174568.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDALKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIW87_001418"
     CDS             complement(345..647)
                     /locus_tag="WIW87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ5174569.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIW87_001419"
     CDS             complement(721..3084)
                     /locus_tag="WIW87_001419"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ5174570.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGEVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3109..3759)
                     /locus_tag="WIW87_001420"
     CDS             complement(3109..3759)
                     /locus_tag="WIW87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ5174571.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(3756..5225)
                     /locus_tag="WIW87_001421"
     CDS             complement(3756..5225)
                     /locus_tag="WIW87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5174572.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(5228..6286)
                     /locus_tag="WIW87_001422"
     CDS             complement(5228..6286)
                     /locus_tag="WIW87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ5174573.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WIW87_001423"
     CDS             complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WIW87_001423"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ5174574.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
BASE COUNT         2067 a         1808 c         1496 g         2214 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagagcgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacga cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacgtccgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttccgg catgaccgcg ccgtaccaca atccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgctg aacacgcggg cgatttcaaa
     1381 cacgcacacg cggttttgtt tgcggttcag attgttttgc agaatttcca ccaagccgcc
     1441 gatgagcgtg gaacgcatca cggcatactg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtaa ccgcgagccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacacctcgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatggttaa tttctctata tttttaagtt attcaataat
     3121 acaataacct ggatgcccaa aactgattct agcttcaggt aaattatttt ttattttctc
     3181 aatcgattgt tggtaataat cggaattgat ttcacaagct gtcaaatcaa actgtgcaat
     3241 gcagcatgca atagcaagag ttccacttcc taaatgtgta tctaaaatct tatcaccctg
     3301 ctttgcatac atttttaaca accactgata taattcgact ggtttttgtg ttgggtgagt
     3361 tttattacga tttttccgca cactaaaccg gaaaatttta gatggcctat cgaatgacga
     3421 ccaagccatt tccgccatag aaaaattatt taatgtctct ggttgattct tatcccaaat
     3481 aataaatcct ttattatatt cacttctcaa ccataactcg ccaaaataat tcccacccca
     3541 aataatttga ttttttgaca cgcgaaataa ttcattaaag taagtttggt ctggcttaat
     3601 atcccattgt gagtattcat tcatattgaa tttataatca ccaccacgtt tagttttatt
     3661 caaaatccca taaggaggat ctacaattgc caaatcaaaa tacttgtcag gataccgaga
     3721 cattaagatc atgttatctt catttgaaat agttatcatg tactatacgc ttccattgtg
     3781 gtgttacttg caaactgttg catagtcaaa ttggaaattt ctgaactaat atcttttcca
     3841 agatttttaa taataatttc atcaaaatac cgataatcaa tctctggaat actggagcga
     3901 ttaatcaaac aattatataa aaattcagca aatgtattgg cacgaataat tacaattggg
     3961 caattacgaa tatcggataa tacggcaggg acataacgtg gtgtgacaac aattgtgtaa
     4021 atggccttaa ttttattttt atgatcttcc aatcttcctg aattaatacc tgataactta
     4081 tttttagttg attttgcctc cactgcaaat tttctctttt gcgtaatata taagcactct
     4141 aaatccgtat tacctggtcc accaattttt ttagcttcta cgttataaaa catattgaac
     4201 cccatttcta gaacatcttc aaatagatat gcctctgttc cattattatt atctgcatat
     4261 tgttcaatca acctaggtaa gtgcaataat tcatatgctg cttcatcctt aagctcacca
     4321 atttcctcca ataactcttt tggatagaag ctataaattt ccttaatgac atcaattttc
     4381 aaacgatcac tatcgttcag atttaatggc atttctgaaa acgaataagc actttgcaat
     4441 tttttcacaa attcctgaag ttgcttagga atcgaaaact cacttgatgt tactttacga
     4501 tatgtttcgg tcttaccatg ctttagacgg caaataattt taccatctgt tttttgcaaa
     4561 acacctgcat cagtcaatgt ttttgaaaaa taagattccc actcatgtgc tgcatttaca
     4621 taagcatgac tacgactatt ttccgcagtt aattgatgtt taatttttac ttcagcacat
     4681 gttcgtaaca ccaaaatgtc ttgtactaat ttttcatagc tattctgatc agcagatttc
     4741 acaaatgaaa ttaaatagat atattcataa gaaaatagtt tacaatccaa tctttcatca
     4801 gataacaact taaatattag tctcatggga tataattgaa tactttcatc agtcttaatg
     4861 tacggatgag gaaatggtat cgcccacaac atagtaagaa aaattttttg aatatattca
     4921 ttattatcca agtgtttcaa aaataaatta cccaatggac tgaacaggaa tttctgtgta
     4981 acttgcccac acacatcacg ataaccaaac atatagaatg ataattcttt tattttatga
     5041 gtagtagcat ccaatgattt acctgtattt cgtctatggt ataaatctag tttttccaat
     5101 ttttcagata acttagcttt atcctctaca gatataaaag aatttgtttt caaaatacct
     5161 gcaaatttac ataaaatatg aaaattctgt aaatgtttat ctattaccca gcgcgcagtt
     5221 gtcatatcta ttttcctatt ctctctacaa tttttttaat taacaatgga ggaatagatt
     5281 caccaataca ttgccgaatt aataattctg atgtatcgtc gggaatattc caatcaatag
     5341 gtaatgatgt taaaagcatt aactctaatg gtgttaaaac tcttgcatct gaatatgttc
     5401 catcaggcat agaccgtcca ggatgaacat ttaattgtga actgatagcg tcattacgaa
     5461 tagtaatagt tggggcagga gcatcccact ccatacggcg ataagttgta ttataacttt
     5521 taattttttc accatttttt ttctttggat aatgttctat attatcaaaa gcagatcgtc
     5581 ctgttggcgt attttttagc catataatgt gctccggagc atgtgtacgt gcaaaatgcc
     5641 atttcacatt agacttttgt ccagactcaa tactaggcaa aaaactaata gcatcacgaa
     5701 cagaaatggt ttttgtaact ttttccggca ttccccaaat agttgaatgt ttattcatac
     5761 gaataatagc tcgtttacga tgttgtgcaa caccataatc ggcagaatca aaaatatgag
     5821 tttgaatata atattcgcaa ccaaataaat cttgcaaaat tacttctact gtttgtaatg
     5881 tccccttata aggtaactta atttttaaaa aaaatggaac attttcaatc aaaacaaaag
     5941 ttgggcgttt taataaaata aattcgatta ctctgaatat taaatgatta cgtttatcat
     6001 tagccatctc ttgaatgtta cgatttttcc ctgcaacact catgccttga caaggaggag
     6061 aagcaattaa aaaatctaat cgattcggca cgctctgtat taaattttga aacacttcct
     6121 catgaagtat atcacctata atcattttac tttcgggata tagagcttta tataaattag
     6181 cacgttctgg caccaattca tttgcagcta taatcttaat acccgcatta tgcaagtagg
     6241 tttctgcaat ccctgcactc gaaaacaaag aagcccctat catcatagaa ccaaacccaa
     6301 attaactata taatctctac aaagcaataa attatgcctc tttaaacaaa attaggaatg
     6361 taatttcaca acaaaatatc aaatgtttta tatcttacat ttgaattttc ctaagcagcc
     6421 tgaaaaccaa gagtaggctg cttttccata ttcaggcagt ctgcacgatc atttcgcaaa
     6481 ctgcttcaaa aagttcaaat cattatcgaa gaacaggcgc aagtcgttca cgttgtaacg
     6541 cagcatagcg aagcggtcga gaccaatacc aaaggcgaaa ccggtatatt tttcagggtc
     6601 gatattgacg tttttcaaca cgttaggatg taccataccg caaccgccta cttccagcca
     6661 tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg ggaagaaaga
     6721 cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa aatccgtgaa
     6781 cactgctttt aagtcggcaa aagttacgcc ctcttctacc cacaaacctt ccgcctgatg
     6841 gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg gggcgataat
     6901 gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg aagtgtgcgt
     6961 acgcaaaaca tcgccgtttt caacgtaaaa cgtatcctgc atcgcacggg caggatggtt
     7021 tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag gcccgtccgc
     7081 cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca gggttacggg
     7141 atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atatcgaggg cttcggcggc
     7201 aagtcgggct tgcagcttga cttcgttgag ggcttcgcgt ttggcattaa aagccgtctg
     7261 aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt caggcgacat
     7321 ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttaccaagat aacgggcttt
     7381 gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc cttctgcaac
     7441 gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatac cacctgaaac
     7501 aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta ttttcttcac
     7561 ggtcaatcca ttggataaat tttct
//
LOCUS       DAWISC010000099         7527 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWISC010000099 DAWISC010000000
VERSION     DAWISC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.099"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            15..420
                     /locus_tag="WIW87_001424"
                     /pseudo
     CDS             15..420
                     /locus_tag="WIW87_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIW87_001425"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIW87_001425"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ5174575.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="WIW87_001426"
     CDS             1376..1723
                     /locus_tag="WIW87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ5174576.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WIW87_001427"
     CDS             1728..2321
                     /locus_tag="WIW87_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ5174577.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WIW87_001428"
     CDS             2385..2993
                     /locus_tag="WIW87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ5174578.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3633
                     /locus_tag="WIW87_001429"
     CDS             3001..3633
                     /locus_tag="WIW87_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174579.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     AAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3673..4452
                     /gene="map"
                     /locus_tag="WIW87_001430"
     CDS             3673..4452
                     /gene="map"
                     /locus_tag="WIW87_001430"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ5174580.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4607..4906
                     /locus_tag="WIW87_001431"
     CDS             4607..4906
                     /locus_tag="WIW87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ5174581.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5144..5518
                     /locus_tag="WIW87_001432"
     CDS             5144..5518
                     /locus_tag="WIW87_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ5174582.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTDNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5706..7172)
                     /locus_tag="WIW87_001433"
     CDS             complement(5706..7172)
                     /locus_tag="WIW87_001433"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ5174583.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1927 a         2264 c         1851 g         1485 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgactgcaa ggctgcggcg gcaggccacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcaagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctt gctgctgttt gccgccgtcg ccggacacgt cgccatggtc
      361 gtcgcccacc gtgttcaggg tagagatgtt ctgtgccgca tgacgggtcg tgtccgctga
      421 ttccgttcac actatggtgc cggctcgtcc ggcactattt gtttttccaa gacagagcca
      481 gatcgtacaa agctttcttt ccctcgcccg tgattttggc agcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgttctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tccttccaca ccggccacgc tcaaagccgc catcaccgcg cttgcgccca
      961 cgacgggaac gactttaaac ccggcctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacactg acgagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgtg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcctgcggc aacggcggtt cggctgccga cgcgcaacac ttcgccgccg
     1921 aaatgaccgg ccgttttgaa aaagaacgca tggaactcgc cgctgtcgcg ctgacaacag
     1981 acacttccgc gctgacagcc atcggcaacg actacggttt cgaccacgta ttcagcaaac
     2041 aggtgcgcgc gctcggacgt gcaggcgatg tattggtcgg catttccacc tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgcct
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttgctca
     2221 acgttcccca tccgcgcacc gcccgtattc aagaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagca cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgt tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactatat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcgccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacgc ccaaagaaaa ccataccaaa ccggacaccc cgaaaaacac gccgcccaaa
     3601 ccccataaag aaattctcga caacctcttc tgacccggca cggcaggcac acccgcaatc
     3661 caaggaagca ttatgaacgg catcatcatc aaaacccccg aagaaatcga aaaaatgcgc
     3721 gagctgggca aactcgtcgc cgaagccctc gactacatcg gacaattcgt caaacccggc
     3781 gtaaccaccg acgaaatcga caaactcgtt tacgactacc acgtcaacgt ccaaggcggc
     3841 tatcccgccc ccctgcacta cggcaacccg ccctacccca aatcctgctg cacctccgtc
     3901 aaccacgtca tctgccacgg cattcccgac gacaagccgc tcaaagaagg cgacattatc
     3961 aacatcgacc tcaccatcaa aaaagacggc ttccacggcg actccagccg tatgtttacc
     4021 gtcggcaaag tctcccccat cgcccaacgc ctgatcgacg taacccacgc ctccatgatg
     4081 gcgggcatag aagccgtcaa acccggcgcg acactgggcg acgtaggtta cgcctgccaa
     4141 caggttgccg aaaacgccgg ctattccgtc gtacaggaat tctgcggaca cggcatcggg
     4201 cgcggtttcc acgaagcccc gcaagtgttg cactacggaa aaaaaggaca gggccccgtt
     4261 ctaaaaccgg gtatgatttt taccgtcgaa ccgatgatca accaaggcaa acgccacctg
     4321 cgtatcctca acgacggctg gacggtggtt accaaagacc gctccctctc cgcccaatgg
     4381 gaacacgaag tcttggtgac cgaaaccggc tacgaaatcc tcaccgtcag ccccgcctcc
     4441 ggcaaaccct gaaaccggac gtatccgccc cataaaaaca aacaatgccg tctgaaagaa
     4501 acggcagata tgatatataa tataaaaaca ggcttgaccc ggcacattac gaaaacaaag
     4561 caaatcggaa tttgccccgc aaccagacaa acttaaagga agttttatga aaatatttga
     4621 aaatatagaa gatgttaaag ccatccgtaa aaagaccggg ctgaaccaga tagacttctg
     4681 gggcaaggtc ggcgttaccc agtccggagg atcgcgctac gaaaccggcc gcaaaatgcc
     4741 caaacccgta cgcgaactgc tccgcctcgt ccatatcgaa tgcatcgatt tggcgaaagt
     4801 caacaaaaaa gatatggaaa tcgccgccct gttgaaaaaa caccatcccg acctgtatgc
     4861 cgagttgtcc aaacagacca agtccgaaag aaaaaaacaa agttaaaccg caacctccgg
     4921 atgcccgaca gtttttcatt tccgaaaaac gcaaacaatg ccgtctgaaa caccggacag
     4981 gtcgccgtat cccgcctgcc gcccctgcct caaaccgccg aaccgcccga acccgccttt
     5041 ttacaaactt tatccaattt cctgtttatt tcgggatacg ccgacattag aatgtcaaac
     5101 agctcgaaac gggcaaactc cacatccatc caaaggaata aaaatgaaac ttctgaccac
     5161 cgcaatcctg tcttccgcaa tcgcgctcag cagtatggct gccgccgctg gcacggacaa
     5221 ccccactgtt gcaaaaaaaa ccgtcagcta cgtctgccag caaggtaaaa aagtcaaagt
     5281 aacctacggc ttcaacaaac agggtctgac cacatacgct tccgccgtca tcaacggcaa
     5341 acgcgtgcaa atgcctgtca atttggacaa atccgacaat gtggaaacat tctacggcaa
     5401 agaaggcggt tatgttttgg gtaccggcgt gatggatggc aaatcctacc gcaaacagcc
     5461 cattatgatt accgcacctg acaaccaaat cgtcttcaaa gactgttccc cacgttaatc
     5521 aggcaacaaa aaacagcgtt ttcagaaatg aaaacgctgt ttttttgacc gttccattat
     5581 tcacaaaagg gaaaaaacga ttacctgccc cgtgtatcaa aacctgccct gccggatgaa
     5641 gggcataacc ggcagggacg gcgtcaacac catatggggg tacggctttt cttgaaagat
     5701 tcggcttaaa tatccaatac tttcgcggta taggcgataa tttcatccgc cctttcaggg
     5761 ttttcgttca acttgatgcc gtaacccggt accagctctt tcagacggtc ttcccaagac
     5821 ggggcgcgct cggggaagca ttggtgcatc agccggatca tcagcggcac agcggtcgat
     5881 gcgcccggcg acgcgcccag caatgcggcg agtgagccgt cggcgtgggc gacaatctcc
     5941 gtaccaaact ggagcacgcc gcctttttcg gagtcttttt taatgatttg gacgcgttgc
     6001 cctgcggtga tgagttccca gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag
     6061 gcgaagcgtt cttctttggt tttacgcaat tcgcccagca ggtatttggt cagcggcata
     6121 ttcgcccagc cggcgcacag cataggatag aggttgtcca tatggatgga cagcggcaaa
     6181 tccataagcg agccttgctt gaggaagttg gaacggaagc ctgcgtaagg gccgaacata
     6241 aggtggcgtt tgccgtccac gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg
     6301 ccgacggaag cctgcccgta cactttggcg ttgtgttgtt cggcggtttc ggggttgctg
     6361 ttgcggaaga acaggccgga cacggggaag ccgccgtagc ctttgccttc ggggatgccg
     6421 gatttttgca gcagggtcag cgcgccgccg cccgcgccga ggaagaggaa gcgggtacgg
     6481 agggtgagct gcccgtcggg gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg
     6541 gattcgcgtt tgatgtcttc gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc
     6601 aaatatttca ccatttggcg cgtcagccgt ccgaaatcga catccgtacc ttcggcggag
     6661 tagttggcgg cgacgggttg gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg
     6721 gaaattttgt tccgatcggt ggaaaattcc atattttcaa aaagtttttg ggttttaaac
     6781 gcgtcataac gtttttgaag ataagaacaa tggtcttcat tcatcaccaa agacatatgc
     6841 ggcacggcat tgatgaagga attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa
     6901 aactggcggc tgacatgaaa ctgttcggca atattgaggg cgcgcgccgg atcgataatc
     6961 ccatttgcac ccaacggcgc atagttcaat tcgcacagcg cggaatgccc cgtgccggcg
     7021 ttgttccacg cgtttgacga ttccaacgcc acatcttcca agcgttcaat cagggtgatt
     7081 tcccaagacg gttcgagttc tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg
     7141 cccaccaaga caacgtctgt cgcttcagcc atggtttact cctaaaaaac aggcatcttc
     7201 tgcccttatg gttatttgcc gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg
     7261 caatggtgtg tgtccgagta tgctgtttcg gggttggaat gcgttgcaag catggcttcc
     7321 gacaccgctt caggggcttg taatatgtta tcgtgaatgt agtggatttt actgggaaat
     7381 gcaaagtttt tctgtcgccc gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt
     7441 atttatctat atatatcaaa tttttaatag ataaaaaatc aaaattgttt atatattaat
     7501 ttttaaaaga ttgtcagcat attgcgt
//
LOCUS       DAWISC010000100         7513 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWISC010000100 DAWISC010000000
VERSION     DAWISC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.100"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            26..2041
                     /locus_tag="WIW87_001434"
     CDS             26..2041
                     /locus_tag="WIW87_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ5174584.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKLGSDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWNERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(2156..3112)
                     /locus_tag="WIW87_001435"
                     /pseudo
     CDS             complement(2156..3112)
                     /locus_tag="WIW87_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3348..4313
                     /locus_tag="WIW87_001436"
     CDS             3348..4313
                     /locus_tag="WIW87_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5174585.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFKKAEPVAAGKK"
     gene            4870..5193
                     /locus_tag="WIW87_001437"
     CDS             4870..5193
                     /locus_tag="WIW87_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ5174586.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFHLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            5307..7484
                     /locus_tag="WIW87_001438"
     CDS             5307..7484
                     /locus_tag="WIW87_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ5174587.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRLTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYTRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1680 a         2281 c         2158 g         1394 t
ORIGIN      
        1 acctgcccaa ccctcaagga ccccgatgaa atcctacccc gacccctacc gccattttga
       61 aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcgc
      121 gcgtttttta gaaaacgaca aggcgcgcgc gctttcagac ggcattttgg cgcagttgca
      181 ggacacgcgg cagattccgt tttgtcagga acaccgcgcg cggatgtacc atttccatca
      241 ggacgcggag tatccgaagg gcgtgtaccg cgtgtgtacc gcggcgacgt atcgttccgg
      301 ctatcccgag tggaaaatcc tgttttcggt ggcggatttc gacgaattgc ttggcgacga
      361 tgtgtatttg ggcggcgtgt cgcacttggt ggaacagccc aaccgcgcgt tgttaacact
      421 gagcaaattg ggcagcgata cggcgtacac gctggaagtg gatttggaag caggggagtt
      481 ggtcgaaggc ggttttcact ttccggcagg caaaaaccat gtgtcgtggc gcgatgaaaa
      541 cagcgtgtgg gtgtgtccgg cttggaacga acgccagttg acccaatcgg gctatccgcg
      601 cgaagtatgg ctggtggaac gcggcaagag tttcgaggaa agcctgcctg tgtatcaaat
      661 cggcgaagac ggcatgatgg tgaacgcgtg gcgttatctc gatccgcagg gttcgccgat
      721 tgatttgatt gaagcgtcgg acggttttta caccaaaacc tatttgcggg tctcagccga
      781 aggcgaggcg aaaccgttaa acctgcccaa cgattgcgac gtggtcggct atctggcggg
      841 gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg aaccaaagct atccgagcgg
      901 cgcgctggtg gcggtgaagc tgaatcgggg cgaactcggg gcggcgcagc ttttgtttgc
      961 gcccgatgaa acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg tggtggcgag
     1021 cctgttggag aacgtacaag gccgtctgaa agcatggcgg tttgccgacg gcaaatggca
     1081 ggaagtcgaa ttgccgcgcc tgccttcggg cgcgttggaa atgaccgacc aaccttgggg
     1141 cggcgacgtg gtttaccttg ccgccagcga tttcaccacg ccgctgacgc tgtttgcgct
     1201 ggatttgaac gtgatggaac tgaccgtcat gcgccgccag ccgcagcagt ttgattcaga
     1261 cggcattaac gtgcagcagt tttggacgac ttcggctgac ggcgagcgca ttccttattt
     1321 ccacgtcggc aaaaacgccg cgcccgacat gccgacgctg gtctatgcct acggcggttt
     1381 cggcattccc gaattgccgc attatctggg cagcattggc aaatattggc tggaagaggg
     1441 caatgccttt gtattggcga acatccgcgg cggcggcgag ttcggcccgc gctggcatca
     1501 ggcggcgcag ggaatcagca aacataaaag cgttgatgat ttattggcag tcgtgcgcga
     1561 tttgtccgaa cgcggtatca gttcgcccga acacatcggc ttgcagggcg gcagcaacgg
     1621 cggactgatt actgccgccg ccttcgtgcg cgaaccgcaa agcatcggcg cgctggtgtg
     1681 cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc tccgccggtt caagctggac
     1741 agacgaatac ggcaatccgc aaaaatacga agtctgcaaa cgccggttgg gcgaattgtc
     1801 gccgtatcac aatctttcag acggcatcga ttatccgccc gcgctcatta ccaccagcct
     1861 gtccgacgat cgcgtccatc ccgcccacgc gctcaagttc tacgccaaac tgcgcgaaac
     1921 ctccgcgcaa tcttggctct actcgcctga cggcggcggc cataccggca acggcaccca
     1981 acgcgaatcc gccgacgaac tcgcctgcgt cttgctgttt ttgaaagagt ttttgggcta
     2041 agggcggggg agcggcactg ccgccgcgaa tgaaaaaagg tcgtctgaaa ctgctttttc
     2101 agacgacctt ttttaatggt tgtttcaaat caaaatatct atgccgccgg ccccatcagc
     2161 acttcttcac atccgaaggc aaaaatccgt aatgccgtct gaacgcttcg ttgaaccgtc
     2221 ccgcgtggcg gtagccgcaa aagtgcatgg cggcttggac ggtgctgccg gattcgatga
     2281 gggcgagcgc gtgttccagc cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt
     2341 gcgctttgaa atagcgtttc aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg
     2401 cgatggtcag cggacgggcg aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc
     2461 gacggcggta accgttgtcg tggcggcgga aggtgaagcg caataatcgg gcggagagtt
     2521 ccagcgcggc ggcttcgtcg gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca
     2581 gcagtgggca ggccgccgcc gtcagtgccg ctgcgttttg cgccagccgt tgcagggcga
     2641 atcggcctat tgtttgcggc gaaaacaggc gttcgtccag caagccttcg tcgtgccagc
     2701 ggcgcagttt ttccagcgaa aaatccaaat gcagcgcgca catgccgctg ttgtcgggca
     2761 gcagggtttc ggatacgtcc gccaaatcgc cgcgtaccag tcagatttcg ccggcagatg
     2821 ggcggtattc cctgccgccc atttgtaacc ggttctgccc cgacaccatg acgaacaagg
     2881 cgcagttgtg gctgaaattg tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca
     2941 tccgcgacaa ggtgatgccc gaatcgaagc ggttgatgca catttccaga tgcaaaccgg
     3001 gctgttttgc ctgcgcaatg agcgcgctgt cggaacagcc gtccaacgcc cagccggatt
     3061 tatcggagcg gacataggtt tggtactggc ggtagatggc ggcggtgttc atgattggat
     3121 aggaacgagt tgtctaacaa atgaattaaa taggaattat taccaataat caagcgcagg
     3181 gattggttga aacggaaaag gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg
     3241 ggaatttgtt ttgccgtatc gggaattttg cgttttgcgg cgtggtttct gcaggttgtt
     3301 tgcttaataa taaacattct tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt
     3361 tttattgggc agcctgcgcc gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa
     3421 aaactgtatc cgccgcatcc gcatctgccg ccacgctgac cgtgccgacc gcgcggggcg
     3481 atgccgttgt gccgaagaat cccgaacgcg tcgccgtgta cgactgggcg gcgttggata
     3541 cgctgaccga attgggcgtg aatgtgggcg caaccaccgc gccggtgcgc gtggattatt
     3601 tgcagcctgc atttgacaag gcggcaacgg tggggacgct gttcgagccc gattacgaag
     3661 ccctgcaccg ctacaatcct cagcttgtca ttaccggcgg gccgggcgcg gaagcgtatg
     3721 aacagttagc gaaaaacgcg accaccatag atctgacggt ggacaacggc aatatccgca
     3781 ccagcggcga aaagcagatg gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg
     3841 cggaattgaa ggcgcagatt gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca
     3901 aaggacgcgg gctggtgctg tcggttacgg gcaacaaggt gtccgccttc ggcacgcagt
     3961 cgcggttggc aagttggata cacggcgaca tcggcctacc gcctgtagac gaatctttac
     4021 gcaacgaggg gcacgggcag cctgtttcct tcgaatacat caaagagaaa aaccccgatt
     4081 ggattttcat catcgaccgt accgccgcca tcgggcagga agggccggcg gctgtcgaag
     4141 tattggataa cgcgctggta cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca
     4201 tgcctgccgc gaactacatt gtcgcgggcg gcgcgcggca gttgattcag gcggcggagc
     4261 agttgaaggc ggcgtttaaa aaggcagaac ccgttgcggc ggggaaaaag tagggagtcg
     4321 tctgaaaacg gagcttccga aggaagcggg gggtttctgc gaagctaaag tgcggtttca
     4381 acgaattgaa aagcagcctg tatgttgaaa ataccgctca agcaaaccta cggtttgccg
     4441 ccctctccct agccctctcc cacagggaga ggggattggg ttgcaggctg cctttaaggt
     4501 ttaggcaaat ttttaacttc gttgaagctg cgatttcaga agctccgttt tagcttcgca
     4561 gaaactccgc ttccttcgaa agctccgttt tcagacgacc ttttggagta ccgcaggcac
     4621 acgcatcgaa cggctgaatc aaagattcag accgatggca gtccgcaccc gagtttatgc
     4681 ggcaaacagc gaggctacgg caacccgccc cctctccctg tgggagaggg ttagggagag
     4741 ggcggtaagc cgcaggctta catcaaagcc gataacgctt ccgttacaac tccgcccact
     4801 gaaagcagcc tgcaacgaag ccaaaacgac aaaccgcatc gtaaaccacc caacccatag
     4861 gagaacccca tgcaaaacga aaccatcaac ctgaaacagc accttgccgc catcaaagaa
     4921 tactggcagc ccgaaatcat caaccgccac gggttccaat tccacttggt caaacttttg
     4981 ggcgattacg gctggcatac gcacggatac agcgacaaag tgctgtttgc cgtggagggc
     5041 gacatggcgg tggacttcgc cgacggcggc agcatgacga tacgcgaggg cgagatggcg
     5101 gtcgtgccga agtcggtgtc gcaccgcccg cgttcggaaa acggctgctc gttggtgctg
     5161 attgagttgt ccgacccgtc cgaggccgtc tgaaaacgaa gtttccgaag gaagctgagt
     5221 ttctgcgaag ctaaaagcag cctgcacctt caatcaatat gccgaaaata caaccccacc
     5281 gcacaccaac acacaaagga aatcccatga cacgcttcaa atattccctg ctgtttgccg
     5341 ccctgttgcc cgtgtacgcg caggccgatg tttctgtttc agacgacccc aaaccgcagg
     5401 aaagcactga attgccgacc atcaccgtta ccgccgaccg caccgcgagt tccaacgacg
     5461 gctacactgt ttccggcacg cacaccccgc tcgggctgcc catgaccctg cgcgaaatcc
     5521 cgcagagcgt cagcgtcatc acatcgcaac aaatgcgcga ccaaaacatc aaaacgctcg
     5581 accgcgccct gttgcaggcg accggcacca gccgccagat ttacggctcc gaccgcgcgg
     5641 gctacaacta cctgttcgcg cgcggcagcc gcatcgccaa ctaccaaatc aacggcatcc
     5701 ccgttgccga cgcgctggcc gatacgggca atgccaacac cgccgcctat gagcgcgtag
     5761 aagtcgtgcg cggcgtggcg gggctgctgg acggcacggg cgagccttcc gccaccgtca
     5821 atctggtgcg caaacgcctg acccgcaagc cattgtttga agtccgcgcc gaagcgggca
     5881 accgcaaaca tttcgggctg gacgcggacg tatcgggcag cctgaacacc gaaggcacgc
     5941 tgcgcggccg cctggtttcc accttcggac gcggcgactc gtggcggcgg cgcgaacgca
     6001 gccgcgatgc cgaactctac ggcattttgg aatacgacat cgcaccgcaa acccgcgtcc
     6061 acgcaggcat ggactaccag caggcgaaag aaaccgccga cgcgccgctc agctacgccg
     6121 tgtacgacag ccaaggttat gccaccgcct tcggcccgaa agacaacccc gccacaaatt
     6181 gggcgaacag ccgccaccgt gcgctcaacc tgttcgccgg catcgaacac cgcttcaacc
     6241 aagactggaa actcaaagcc gaatacgact acacccgcag ccgcttccgc cagccctacg
     6301 gcgtagcagg cgtgctttcc atcgaccaca acaccgccgc caccgacctg attcccggtt
     6361 attggcacgc cgacccgcgc acccacagcg ccagcgtgtc attgatcggc aaataccgcc
     6421 tgttcggccg cgaacacgat ttaatcgcgg gtatcaacgg ttacaaatac gccagcaaca
     6481 aatacggcga acgcagcatc atccccaacg ccattcccaa cgcctacgaa ttttcccgca
     6541 cgggtgccta cccgcagcct gcatcgtttg cccaaaccat cccgcaatac ggcaccaggc
     6601 ggcaaatcgg cggctatctc gccacccgtt tccgcgccgc cgacaacctt tcgctgattt
     6661 tgggcggacg atacacccgt taccgcaccg gcagctacga cagccgcaca caaggcatga
     6721 cctatgtgtc cgccaaccgt ttcaccccct acacaggcat cgtgttcgac ctgaccggca
     6781 acctgtctct ttacggctcg tacagcagcc tgttcgtccc gcaatcgcaa aaagacgaac
     6841 acggcagcta cctgaaaccc gtaaccggca acaatctgga agccggcatc aaaggcgaat
     6901 ggcttgaagg ccgtctgaac gcatccgccg ccgtgtaccg cgcccgtaaa aacaacctcg
     6961 ccaccgcagc aggacgcgac ccgagcggca acacctacta ccgcgccgcc aaccaagcca
     7021 aaacccacgg ctgggaaatc gaagtcggcg gccgcatcac gcccgaatgg cagatacagg
     7081 caggttacag ccaaagcaaa acccgcgacc aagacggcag ccgcctgaac cccgacagcg
     7141 tacccgaacg cagcttcaaa ctcttcactg cctaccactt tgcccccgaa gcccccagcg
     7201 gctggaccat cggcgcaggc gtgcgctggc agagcgaaac ccacaccgac cctgccacgc
     7261 tccgcatccc caaccccgcc gccaaagccc gcgccgccga caacagccgc caaaaagcct
     7321 acgccgtcgc cgacatcatg gcgcgttacc gcttcaatcc gcgcgccgaa ctgtcgctga
     7381 acgtggacaa tctgttcaac aaacactacc gcacccagcc cgaccgccac agctacggcg
     7441 cactgcggac agtgaacgcg gcgtttacct atcggtttaa ataaggtcgt ctgaaaacgg
     7501 agtttctgcg aag
//
LOCUS       DAWISC010000101         7497 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWISC010000101 DAWISC010000000
VERSION     DAWISC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.101"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(16..953)
                     /locus_tag="WIW87_001439"
                     /pseudo
     CDS             complement(16..953)
                     /locus_tag="WIW87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1168..2280
                     /gene="nadA"
                     /locus_tag="WIW87_001440"
     CDS             1168..2280
                     /gene="nadA"
                     /locus_tag="WIW87_001440"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ5174588.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKETNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            2438..2767
                     /locus_tag="WIW87_001441"
     CDS             2438..2767
                     /locus_tag="WIW87_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ5174589.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            2816..4340
                     /gene="nadB"
                     /locus_tag="WIW87_001442"
                     /pseudo
     CDS             2816..4340
                     /gene="nadB"
                     /locus_tag="WIW87_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            complement(4518..5183)
                     /gene="pgmB"
                     /locus_tag="WIW87_001443"
     CDS             complement(4518..5183)
                     /gene="pgmB"
                     /locus_tag="WIW87_001443"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ5174590.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(5196..7454)
                     /locus_tag="WIW87_001444"
     CDS             complement(5196..7454)
                     /locus_tag="WIW87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ5174591.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGSTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         1549 a         2070 c         2174 g         1704 t
ORIGIN      
        1 tcgtctgaaa acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc
       61 gggcaggacg tcgtcgcgga agcggcaaag ctgcgcggga cggcctttgc tgcccgatac
      121 gccggtatcc gacggctcga tgaggttttg ctgctgaatc tggcggcgga agttttgctt
      181 gtgcagcaat ctgccgctga tggcttcgac gctgttttgc agttgcagca gcgtgaattc
      241 gggcggcatc agttcaaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgagacag
      301 ggcggtcgcc agtacgcggc ggtggtcgtg gcgcatgggc tgccccgtaa gcgcgaagtc
      361 gaagtttgcc tgcggctcgg cggcttccgc tatcaggccg ctttcataca gcatttcata
      421 gcgttgcaaa acgtattctt ccgaccagtt ttccggttcg acccccctgc acaaatgaat
      481 gcgcttgagc cgcttttggc gcacttcctc cgtgtccgcc gagtttgccc aaatgcgcag
      541 gcggccgacg acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc acgggaaata
      601 gccgtagcag tcctgccatt tcgcatccgg gtgcaggatg cttcggctgc ctcgcgcacc
      661 agccccaaat agctgacgta cagcacgggc atgccgtgtt cgttgcggcg gtgggtatcg
      721 acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt ggcgacccac
      781 agttttacgc ctgcctgcaa ggaattgcgc aggggggaga gcgggccgtt gggcaggagc
      841 atgccttggg cgacggtcaa tacccgcagg ccgccgtcgg taacggcaat caatacggga
      901 accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc catgctcggc
      961 tttctgcggg tgggaaaggc tttattgtaa accaaagcgg tttgtcggaa cgaagcgggt
     1021 cggttttacg gtattgcaaa cctgttttca gacgaccttg catattatac tcaagtatta
     1081 tactcaagtt gagcataatg taaaaacagt ttgaaaaaca gacggcaaac cttcatgccg
     1141 tctgaaaaac agctacaagg aaacatcatg caaaccgccg cccgccgctc gttcgattac
     1201 gatatgcccc tcatccaaac gccgacttcc gcctgccaaa tccgtcaggc gtgggcgaaa
     1261 gttgccgaca cgcccgaccg cgagacggca ggtcgtctga aagacgaaat caaggcattg
     1321 ctgaaggaga caaacgcggt cttggtggcg cattattacg tcgatccgct gattcaggat
     1381 ttggctttag aaacgggcgg atgcgtgggc gattcgctgg aaatggcgcg cttcggcgcg
     1441 gaacacgaag ctagtacatt ggtggtggcg ggtgtgcgct tcatgggcga gagcgcgaaa
     1501 atcctctgcc ctgaaaaaac agtgctgatg cctgatttgg aggcggaatg ttctttggat
     1561 ttgggctgtc cggaagaggc gttttcggcg ttttgcgacc aacaccccga ccgcacggtc
     1621 gtagtgtacg ccaacacttc cgccgccgtg aaagcgcgtg ccgactgggt ggtaacgtct
     1681 tcggtggcgt tggaaatcgt gtcgtatctg aaatcgcgcg gcgagaagct gatttggggg
     1741 cccgaccgcc acctcggcga ctacatccgc cgcgaaacgg gcgcagatat gctgttgtgg
     1801 cagggttcgt gcatcgttca caacgaattc aaagggcagg aactggcggc gttgaaagcg
     1861 gaacaccccg acgcggtggt gctggttcat cccgaatcgc cgcaaagcgt catcgaactg
     1921 ggcgacgtgg tcggctcgac cagcaagcta ctgaaagccg ccgtatcgcg ccctgaaaaa
     1981 aaattcatcg tggcaaccga tttgggcatc ctgcacgaaa tgcaaaagca ggcgcccgat
     2041 aaagaattca tcgccgcacc gacagcgggc aacggcggaa gctgcaaaag ctgcgcgttc
     2101 tgcccgtgga tggcgatgaa ttcgctgggc ggcatcaaat acgccctgac aagcggacac
     2161 aacgaaatcc tgttggacag aaagctgggc gaagccgcca aactgccttt gcagcgtatg
     2221 ctcgatttcg cggcaggact aaagaaaaaa gatgtgttca acggcatggg ccccgcctga
     2281 tttgtcgttc gtaatgggtg cagcctgaaa ataccgcccg cctgctttcc caagacggga
     2341 tttgcacaca aaaaatcttt aacaacaaga ctgttttttt attttattgc attgatataa
     2401 ttatactttc tttcatttat caggtaaaaa catgcaaatg cactggctct ttctgactgt
     2461 agcaatttta agcgaagtct gcggttcttc catgctcaaa ctgagtggcg ggtttagcaa
     2521 actgtggcct tctattggcg tggtagtcag cttttcggtg tgtttttggg ccttgtctat
     2581 gacactgaaa accatgccgc tggctacagc atacgccatt tgggcaggcg tgggactggt
     2641 tttaacggct ttagtcagcg tggtgttttt cggtgagaaa gctgatttca ttgggattgt
     2701 cagcatcggt ttgattttgc tgggcgtggt gcttctcaat accatgtcgc atatgtcggg
     2761 gcattgatta atccactata ccgtttcaga cggcctctca aacaaggaca acaccatgca
     2821 aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc tcgccctgtc
     2881 gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg acaccgccag
     2941 ccgtcatgcg caaggcggga ttgcggcggc gtggtcggga gaggacgaca tcgaaaaaca
     3001 cgtcgccgat actttggaag cgagcgcagg tttgtgcgac gaagccgccg tccgcaccat
     3061 cctgtcgcag ggcaaaccgg caatcgaatg gctgctggcg cagggcgtgg cgttcgaccg
     3121 aaattatgac ggcctgcacc tgacgcgcga aggcgggcac acctgccgcc gaatcgccca
     3181 cgtcgccgac tacacgggcg aagccgtcat gcagagcctg cttgcccaaa tacgccgccg
     3241 cccgcacatc cgcgtttgcg agcggcagat ggcgttggac attcaaaccg aatcaggtgt
     3301 ggcgtgcgga ctgatcgtcc ttgaccgccg aacgcaagaa acctacctca tccgcgcccg
     3361 ccataccgta ctcgcaggcg gcggcttggg acagatttac gccgccacca ccacgccgcc
     3421 cgaatgcacg ggcgacgcca tcgccatggc gatacgcgca ggctgcgcag ttggaaacct
     3481 cgaatttatc caattccacc ccacaggctt ggcaagatcg tctgaaaacg gccgtacctt
     3541 cctgatttcc gaagccgtgc gcggcgaagg cggcatcctg accaaccaag cgggcgaacg
     3601 gtttatgccg cattacgacc gccgcgccga actcgcgccg cgccgaactc gcgccgcgcg
     3661 acatcgttgc ccgcgccatc gccgccgaaa tcgccaaaca aactccagac ttcgtctcgc
     3721 tcgacatcag ccgccaaccc gaagcgttcg tccgccggca ttttccgtcc atccatcggc
     3781 actgtttatc ccaatgcggc ctggacatca cgcgccaagc catccccgtc cgccccgtgc
     3841 aacactacac ctgcggcggc atccaaaccg acccctgcgg cagaacctcc ctgccgcagc
     3901 tctacgcctt aggcgaaacc gcctgcaccg gcttgcacgg agccaaccgc ctcgccagca
     3961 actccctgct cgaatgtgtc gttaccgcca gacttgccgc ccaaaccatc gcggacggac
     4021 aagcattcca agccgaacca ttccaaaggc cgtctgaaag cctctccgcc gaagcaggca
     4081 tctttgcaga cgaccttcaa aacacattca gccgccccgt cctgcaaacg ttcaaccaac
     4141 gccatctcgg catcctacgc agcgataccg gcctgcgccg cgccatcgcc caactgcggc
     4201 tttggaagca aaaccaagcc gaaccgcaca ccgcgtccga atacgaaaac cgcaacctgc
     4261 tcgaatgcag cctcgccgtc gcccaagccg catacaggcg gagacagaac atcggcgcgc
     4321 attttaatag tgattgttaa ggttggctgg ttagaaaaaa aatcgttcaa ataaatttta
     4381 atagcatggt taagagattg gcattcagtt tttagggttg cgtaagttgg gttatcaatc
     4441 tgttctaaaa gcagaaaggc cgtctgaaac actgttcaga cggcctttta cttccttgac
     4501 actttatctg acgcgtttta cctgcccgac tgtgcccaca cgttttgcag gtaggcgtag
     4561 gtcagctcgg cggtatcgga caccagcgcg atgtcgctgc ccaagtcttc ggctttgccc
     4621 acgccgatag gcaaggcgcc ggcggctttg atggcggcga cacctgcggc agcgtcttca
     4681 atgccgatgc agcggcggat gtccgcatct acgccctcgg ctgctgcgag gaagatgtcg
     4741 ggggcgggtt tggaatgtgc gacggcggca gggtcggcaa tggcgtcgaa gaagtgggtc
     4801 agccccatgc gttccagcag gaacgggccg tttttactgg cggacgcaag ggcgattttt
     4861 ttgccgtttg ccctcaatgc ttccagcagg ggcaaaatgc cgggatacac gtcttcgggt
     4921 ttgactgcct gaatcatctc gacgtagttg tcgtttttgc ggcgggtcag ttcggcgaac
     4981 tcggcttcgc cgacggtttt gccgccgtgt gcgaggatgc gtttgagcga atcgtcgcgc
     5041 gacacgcctt tgagctgctc gttaaacttg cggtctatgc cgatgcccag ttcttcggcg
     5101 agctttttcc atgcgcggta gtggtattcg gcggtgtcgg tgatgacgcc gtcgagatcg
     5161 aagagcactg cggtaaacgt cattttgcgc cctccttatt tttccaacgc ggcggtatgg
     5221 ctgccgttga gtgtgatgtc tttgccgtac acctgcaaat cgagcggctc gcctttgagc
     5281 agggtgaaga cgacgttttc tttgccgacg gcgactttaa tcagacggcc tcggtagttg
     5341 atatggaagg tgtagcctgt ccacgcgctc ggcaggaacg gtgcgaagct gagtttaccg
     5401 ccccaagttt tcatttgggc gaaaccttgg acgatggcga gccacgagcc ggtcatagaa
     5461 gtaatgtgca ggccgtcttc ggtgtcgttg ttgtagttgt ccaagtccag gcgggctgtg
     5521 cgccggtaca tttccacggc tttttcttct ttgcccagtt cggcggcgag aatagagtgg
     5581 atacacggcg acaacgagct ttcatgcacg gtcatcggtt cgtagaagtc gaaattgcgg
     5641 cgtttttcgt cgatattgaa acggtcgccg aagaaataga tgccttgcaa gacatccgcc
     5701 tgtttgatga agggcgaacg caggattttg tcccacgacc atttctggtt gagcggcaaa
     5761 tcgtcgggcg aaagcgcgga cacggggcgg atgtctttgt cgaggaatcc gtcgtgttgt
     5821 acgaatacgc cgagttcttc gtcatgagga cggtacatat tcgcgctgat gtccgcccat
     5881 ttttccaact cggcggcgct cacgttcaaa tccggacgcg ggtatttcgc cagggcttcg
     5941 cgggtgtagt ctaataccca tgcggcgagg gtgttggtgt accagttgtt gttgatgttg
     6001 ttttcgtatt cgttcgggcc ggttacgccg tgaatcatgt atttgccgct gcgtttggag
     6061 aagtggacgc ggtccgccca gaagcgggac acttcgacca agacttccaa gccttctttg
     6121 gcaagatagc tttcgtcgcc ggtgtagttg gtgtagttgt agatggcgta aggaatcgcg
     6181 ccattgcggt ggatttcctc gaaggtgatt tcccattcgt tgtggcactc gatgccggta
     6241 aacgttacca tcggatagag tgcgcccgcc aagccctgtt cgcgcgcatt gtgctgcgcc
     6301 tgcggcagtt ggttgcggcg gtattgcagc aggttgcggg taacttcggg ttccgccagc
     6361 gcgaggtaga gcggtacggc gtaggcttca gtatcccaat aggttgcgcc gccgtatttt
     6421 tctccggtaa agcctttcgg gccgatgttc agtcgcgcgt cttcgccgta gtaggtggag
     6481 aacagttgga acagattgaa acggatgcct tgctgtgctt cgtcgctgcc ttcgatgacc
     6541 acgtcggcga tttcccaacg gtgcagccag cctgctttgt gcgcgtccag caaggtttca
     6601 aacgcaacgc ctgcgacttt ttccgacaaa gcgcggcctg cggctttcac tgcttccaag
     6661 ccctgataat cgcggctggt ggtaacaata acccgttttt caaaggtttc gggtgtgctg
     6721 ccgacttcgg cttcaaaaga attggagacc tgccagtcgg tttgtctgcc gccgagggct
     6781 ttaaagctgc cggcgaaggt ttgctcggcg ttgacgataa attgttccac gccgaaaggg
     6841 ttggcaacgg tttgggcggc aatgtaggag agactgtctg aaacgccttt gtccaatacc
     6901 tgccagaatt tttcttcgta gttggagtct tcgtttttca cgtcggcatc gatgatggaa
     6961 tcgatgcgga cttggtgggt tttgccgtca acggatacgg cttcccagcg gataaccgcc
     7021 agctcttttt gcgcgacgga caggaatttg cacacattga aacgcacacc gaatacggtg
     7081 aacgagcggc gcaacacgcc gtgctgcata tcaagttcga cggagaagcc ggcaacatcg
     7141 tttttcgcca agtccacttc ctgcccgtcg acaaagattt tgactttgct gaaattgagc
     7201 gcgttgatgg ctttaccgaa atatttgggg tagccgtttt tccaccagcc gacgcgggtt
     7261 ttgtcgggaa accacacacc ggcgatgtag gtacccaagt ggctgtcggc ggaataggtt
     7321 tcctcaaagc tgccgcgcat acccatatag ccgttgccca aactggtcag gctctcttgc
     7381 agccgtttgt gttctttttc cagctttgcc gaacgcagcg tccaagggct gatttccata
     7441 attcttgtgt acatttatga agctcctgtt tggattgatt tgagggaatg gtgaaat
//
LOCUS       DAWISC010000102         7494 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWISC010000102 DAWISC010000000
VERSION     DAWISC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.102"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            125..1062
                     /gene="tehA"
                     /locus_tag="WIW87_001445"
                     /pseudo
     CDS             125..1062
                     /gene="tehA"
                     /locus_tag="WIW87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
     gene            complement(1135..1818)
                     /locus_tag="WIW87_001446"
     CDS             complement(1135..1818)
                     /locus_tag="WIW87_001446"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ5174592.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1966..4269
                     /gene="parC"
                     /locus_tag="WIW87_001447"
     CDS             1966..4269
                     /gene="parC"
                     /locus_tag="WIW87_001447"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ5174593.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDCLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4289..5806
                     /locus_tag="WIW87_001448"
     CDS             4289..5806
                     /locus_tag="WIW87_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ5174594.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5858..6325
                     /locus_tag="WIW87_001449"
     CDS             5858..6325
                     /locus_tag="WIW87_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ5174595.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6429..7181
                     /locus_tag="WIW87_001450"
     CDS             6429..7181
                     /locus_tag="WIW87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5174596.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1674 a         2258 c         1974 g         1588 t
ORIGIN      
        1 tcacttggtg cttcagcacc ttagagaatc gttctcttcg agctaaggcg aggcaacgtc
       61 gtactggttt ttgttaattc actatatctg tgcgttacga cggcatgccg tctgaagggt
      121 gtttatgtct gcatctaaga aatttccgat tcctttgagc tatttcagca tcgcgctggg
      181 cttgtttgcc ttggggctgt cgtggcgtta cggcgcgtct gtcgggctgc tgcccgcctt
      241 ggccgccgaa tcgctgcttg cggcggcttc ggtcgtctgg ctcttgctgg tggcggcata
      301 cctgatcaaa atgtttgcgt accgaaacga ttttttgtct gatttacgcg acttggtgca
      361 atgctgcttc atcagcgcga ttccgattac cgctatgctg gagggactcg cgctgaagcc
      421 ctatcaggca ggcgcggcgg cagtcctgat ttatgtcggc gttgccggac agttggcttt
      481 ttcgatgtat cgggcggccg gtctgtggcg cggcctgcat tccttggagg cgacgacgcc
      541 gattatttat ctgcctacgg ttgcgacaaa ctttgtcagc gcgtcatctc tggcggcgtt
      601 ggggcatcat gattatgcag ctttgttttt cggcgcgggt atgttttcct ggctgagctt
      661 ggaagcctcc atcttgggca ggctgcgcac ggcggcaccg gtcggcacgg cggcgcggtc
      721 ggcatccagc ttgcgcccgc ctttgtcggc tgcggcgcgt attttgccgt cggcggtaaa
      781 gtcgacggtt ttgcgttggc attaatcggc tacggctgcc tgcagctttt gttcttgctg
      841 cgcctgaccc gctggttttg ggaaggtggt tttacgatga gcttttgggg attttcattc
      901 ggtttcgcgg caatggcagg atgcggtctg catctggcgg cttccggcgt attgtcgggc
      961 ttggggctga cgcttgccac cgccggatcg gcaggcgtgg cgctgctgct tgtcggtacg
     1021 ctgcaccgga tagcgacggg gcgtttcttg gtacgcagct gatgcgtttt gccgccttgt
     1081 caaaaatgcc gtctgaaacg ctgggattca gacggcattt tttatttcac acccttacag
     1141 gtagaatttt tcgatgactt tcaaattgtc gtccaatttg tacaccaacg gctgaccggt
     1201 cgggatttcc aagcccataa tgtcttcgtc ggaaatgccc tcgatgtgtt ttgccagcgc
     1261 gcgcagggag ttgccgtgcg ccgccaccaa gacgcgtttg ccgctcaaaa tcgcgggggc
     1321 gatttggtct tcccaaaacg gcaatacgcg ctccagcgtt actttcaggt tttcgccgtc
     1381 gggtacgaca tcggcaggca gatgggcata gcggcggtct ttgtgtgcgg aaaactcatc
     1441 gtctttgtcc aaaagcggcg gcagggtgtc gtagctgcgc cgccagatgc ggacttgctc
     1501 gtcgccgtat tgttcggcgg tttgtttttt gtccaggcct tgcagttggc cgtagtggcg
     1561 ttcgttcagc cgccacgttt tgatttgcgg tacgaacagt tggtcggatt cttccaaaac
     1621 gatgttgcag gtcttaatcg cgcgggtcag gacggatgtg aaggcgatgt cgaactcata
     1681 gccgttttct ttcagtttct tgccggcggc ggcagcctcg gcaagcccct gctcgctcag
     1741 cttcacgtcg cgccagcctg taaacaggtt tttcgcgttc cattcgcttt gtccgtggcg
     1801 gataaatacc agttccatat cgtctccaat gtgtgaaagt gggaaagcct tatttataac
     1861 atattttcac atttcccgta tttgattcag attcagacac gcgcccacta tggtttgccg
     1921 ttttgattta caataatgtc ctttgcttta cattccgcat acacaatgaa tacgcaagcg
     1981 cacgccccac ataccgattc caatacgctg atgctcggcc gatacgccga acgcgcctat
     2041 ctcgaatacg ccatgagcgt ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccag
     2101 aagcccgtgc agcggcgcat tttgtttgcc atgcgcgata tgggtttgac ggcgggggcg
     2161 aagccggtga aatcggcgcg cgtggtcggc gagattttgg gtaaatacca cccgcacggc
     2221 gacagttccg cctatgaggc gatggtgcgg atggcgcagg attttacctt gcgctatccc
     2281 ttaatcgacg gcatcggcaa cttcggctcg cgcgacggcg acggggcggc ggcgatgcgt
     2341 tacaccgaag cgcggctgac gccgattgcg gaattgctgt tgtccgaaat caatcagggg
     2401 acggtggatt ttgtgccgaa ctacgacggc gcgtttgacg aaccgctgca cctgcccgcc
     2461 cgcctgccta tggtgttgct caacggcgcg tcaggcattg cggtgggcat ggcgaccgag
     2521 attccgccgc acaatttgaa cgaagtgacg caggcggcga ttgcgttgtt gaaaaagccg
     2581 acgctggaaa ccgccgacct gatgcaatat attcctgccc ccgattttgc cggcggcggt
     2641 caaatcatca cgccggcgga cgaattgcgc cggatttatg aaaccggcaa gggcagcgtg
     2701 cgcgtgcgtg cgcgttatga aatcgaaaaa ttggcgcgcg gacagtggcg cgtcatcgta
     2761 accgagctgc cgccgaacgc caattccgcc aaaatccttg ccgaaatcga agagcaaacc
     2821 aacccgaaac cgaaagcggg taagaaacag ctcaaccaag accagctcaa taccaaaaag
     2881 ctgatgctgg atttaatcga ccgcgtgcgc gacgagtccg acggcgaaca tcccgtgcga
     2941 ctggtattcg agccgaaatc cagccgcatc gataccgata ccttcatcaa cacgctgatg
     3001 gcgcaaactt cgctggaagg caatgtgtcg atgaacttgg tgatgatggg tttggacaac
     3061 cgccccgcgc agaaaaacct gaaaacgatt ttgcaggaat ggctggattt ccgcaccgta
     3121 accgtaacac gccgtctgaa attccgtttg aaccaagtgg aaaaacggct gcacatcctc
     3181 gaaggccgtc tgaaagtctt tctgcacatc gacgaagtga ttaaagtcat ccgcgaatca
     3241 gacgacccga aagccgattt gatggcggcg ttcgggctga ccgaaatcca agccgaagac
     3301 attttggaaa tccgcctgcg ccagttggcg cgtttggagg gtttcaaact cgaaaaagaa
     3361 ttgaacgagt tgcgcgagga acaaggccgt ctgaacatcc ttttgagcga cgaaaacgaa
     3421 aaacgcaagc tgattgtcaa agagatgcag gcggatatga aacaatacgg cgacgcgcga
     3481 cgcacgctgg tggaagaggc cggacgcgcc gtgctgacgc agaccaccgc cgacgaaccc
     3541 atcacgctga tcctgtcgga aaaaggctgg atacgcagcc gcgccggaca caatctcgat
     3601 ttgagccaaa ccgcgttcaa agaaggcgac tgcctcaaac aaaccctcga aggcagaacg
     3661 gttttacccg tcgtcatcct cgattcatcg ggcagaacct acacgctcga tgccgccgaa
     3721 atccccggag ggcgcggcga cggcgtaccg gtttcctcct taatcgagct gcaaaacggc
     3781 gcgaaacccg ttgcgatgtt gacaggattg ccggaacaac attatttatt atcaagcagc
     3841 agcggctatg gcttcatcac caagctgggc gatatggtcg ggcgcgtgaa agcgggcaaa
     3901 gtggtgatga ccgcagacag cggcgaaacc gttttgccgc cggttgccgt ctatgcctcc
     3961 tcgttcatca accccgactg caaaatcatt gccgccacca gtcaaaaccg cgccctcgcc
     4021 ttccccatcg gcgaattgaa aattatggcg aaaggcaaag ggctgcaaat catcggatta
     4081 aacgccggcg aatcgatgac gcataccgcc gtttcttccg agctggaaat cctgattgaa
     4141 agcgaaggca ggcgcggcgc ggcgcacaaa gaccgcatcc ccatctccct gcttgaggca
     4201 aaacgcggca aaaaaggcag actattgccc atatcgggca gcctgaaaca gctttcttcc
     4261 cctaaataaa cccggttccg cacatattat ggtgatttcc aacccccgcg aacttgaaaa
     4321 actcaaagac cggattccca atctgatcaa catcatccgc gtcgccatcg tttttccgct
     4381 gatgattatg cacatcctcg ggctggaaac cggcagccgt gcgaacctgc acgcttcgtg
     4441 gacggcgtgg gcgttttatg tttggctcgc cattgcctgc tggctgattt tcttttccat
     4501 tatccatccg cattggcaat ggcagtcgct gaaaatgccg cgtttcagcg cggtagcgga
     4561 catcacgatg atcggcgtgc tgacctacct gttcggcggc atcgattccg gcttcggcat
     4621 cctgatcctg cccttcgtcg tctgctcctg cctgctcagc tacgggcgct accccctgct
     4681 ctattccagc tacgccgcca tcctgctgat attcaacgcc attgccgacg gcgatatcgg
     4741 caaatacccg ctcatatcgg atgcccgaac cgcctcggca accttcatcc ttgtcgccgc
     4801 ctcctatctt tccgccatct tcacctcact gtcggtcaaa tacatcgacc gtgccggaaa
     4861 actcgcctac gacagccata tcgcctacca ccgcatcaaa ggcttgagcc aaaccgtact
     4921 cgaacgcgtt caggaagctg tcgtcgtcat caatgccgaa gggctggcgg tgctgttcaa
     4981 ccggaaggcg aaagaccttt tccccgcgct cgaaatcgga cggcgcgccg gtctgtccga
     5041 ttctgccgcc gaactgtggg atcaagcctc tccgcacact ttcgaatacg tcctcggcac
     5101 acccggcctg aacgccggca tccgcgccgt tccggtcaac aaagggtcgg acaagctgct
     5161 catcctctac atccgcccgc aaagcgaaat tcaggcagaa gccctgtccg tcaaacttgc
     5221 cgcgctcgga caactgaccg ccaacctcgc ccacgaaatc cgcaacccga tgtccgccat
     5281 ccgccacgcc aacgacctgc tgcgcgaaaa tatggaagcg ggggcggcag atccgttcaa
     5341 cgccaaattg tgcaaaatca tcgacggcaa catctgccgc atcgacaaaa tgctcgaaga
     5401 catttcctcg ctcaacaagc gcaacaaaac cgaacgcgaa accatcggcc tgataccgtt
     5461 ttgggaagaa ttcaaacaag agttcctgct cggccatccc gatgccgccg actgcatccg
     5521 tccggacatt caaggcggca gcccgaccgc ctatttcgat cccgcccacc tgcggcaaat
     5581 tatgtggaac ctcgccaaca acgcgtggcg gcacagccgc aaacagcccg gctcgatttc
     5641 cgtcaccatc cgccccgcgc aaaaaaacac cgtctgtatc ctctttgccg accgcccgaa
     5701 gtgcaggaac acctgttcga acccttttac accacggcgg aaaacggcac cggcctcggg
     5761 ctgtatgtcg cccgcgaact ggcgcacgcc aatttcggcg atttgaccta cctaccggaa
     5821 gccaaatgtt tcgaactcac attaccggaa aaaaccaatg actgaactgc aacaccccgt
     5881 cctcgtcgtc gatgacgaaa ccgacattct cgacctgatg gaaatgaccc tgatgaaaat
     5941 gggcttgcgc gtccataccg cgtcaggcgt tgccgaagcc aaaaacaagc tcgacagcca
     6001 acgctattcg ctcgtcctga ccgatatgcg tatgccggac ggctcggggc tggaagtcgt
     6061 ccaacacatc aacagccgcc tgctcgatac gccggttgcc gtcatcaccg ccttcggcaa
     6121 cgccgatcag gcacaggaag cgttgcgttg cggcgcgttc gaccccgata ccatgcagat
     6181 acaggactat ctcgaccaaa tcgaacgcga catcatcgaa caaaccctca aacaaaccga
     6241 aggcaaccgc acgcaggccg ccaaacgctt gggcatcagc ttccgttcca tgcgctaccg
     6301 tatggaacgc ctcaacatcg gctgacgaca aaacggcatc cgcaccatct ccgcccaccc
     6361 gaaaaaatgc cgtctgaaac ggcacgggaa agcgggttcg ccccacgccc gaacggacac
     6421 aaaacaccat gaccgacatc cttattgaca acaccgccac cgaaaccgtc cgcaccctga
     6481 tacgggcatt cccccttgtg cccgtttccc aaccgcccga acaaggcagt tacctccttg
     6541 ccgaacacga taccgtcagc ctcaggcttg tcggggaaaa aagcagcgtc atcgtcgatt
     6601 ttgcctccgg cgcggcacaa taccggcgca caaaaggcgg gggcgaactc atcgccaaag
     6661 ccgtcaacca caccgcgcac cccaccgttt gggacgcaac cgcaggattg gggcgcgaca
     6721 gcttcgtcct cgcctcgctc gggctggccg ttaccgcctt cgagcaacat cccgccgtcg
     6781 cctgcctgct ttcagacggc atccgccgcg ccctcctcaa tcccgaaacg caaaacaccg
     6841 ccgcgcacat caacctccat ttcggcaacg ccgccgaaca aatgcccgca cttgtccaaa
     6901 cacaaggcaa acccgacatc gtctatctcg accccatgta tcccgaacgc cgcaaaagtg
     6961 ccgccgttaa aaaagaaatg acctacttcc accggctcgt cggcgaagcg caagatgaag
     7021 cggcactcct gcataccgca cgccaaacag caaaaaaacg cgtcgtcgtc aaacgccccc
     7081 gcctcggcga acaccttgcc ggacaagacc ctgcctacca atacacaggc aaaagcaccc
     7141 gcttcgacgt ttacctgccc tacgggacgg acaagggata acgcccataa aacaagacac
     7201 cgaaaaattt gccgttctta tgcaaacgag aaaccggttt ttgcgtttcg actgttttgg
     7261 ataagtcatc acaccttaaa gtttgtcatt cccacagaag tgggaatccg attcattcag
     7321 ttttatagtg gtttaaattt aaaccactat agttgttttc gagtttcagg caacttccaa
     7381 accgtcattc ccacggaagt gggaatctag aaatgaaagg caacaggaat ttatcgtaaa
     7441 tgactgaaac cgaacggact agattcccgc ctacgcggga atgacggggc gggc
//
LOCUS       DAWISC010000103         7386 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWISC010000103 DAWISC010000000
VERSION     DAWISC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.103"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WIW87_001451"
     CDS             complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WIW87_001451"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ5174597.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPVENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSGGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDTGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRATMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAVAFDYQGKRATEAVLLLA
                     EAEQTFPLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2961..3755)
                     /locus_tag="WIW87_001452"
     CDS             complement(2961..3755)
                     /locus_tag="WIW87_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ5174598.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            complement(4505..5374)
                     /locus_tag="WIW87_001453"
     CDS             complement(4505..5374)
                     /locus_tag="WIW87_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ5174599.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLVYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5425..5961)
                     /gene="ruvC"
                     /locus_tag="WIW87_001454"
     CDS             complement(5425..5961)
                     /gene="ruvC"
                     /locus_tag="WIW87_001454"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ5174600.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5964..6203)
                     /locus_tag="WIW87_001455"
     CDS             complement(5964..6203)
                     /locus_tag="WIW87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ5174601.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6233..7243)
                     /gene="dusB"
                     /locus_tag="WIW87_001456"
     CDS             complement(6233..7243)
                     /gene="dusB"
                     /locus_tag="WIW87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ5174602.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1666 a         1937 c         1932 g         1851 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataattaat atgactttgc ggccgttttg
       61 ccattgcgta ataaaacgat ggggaagtga tgataaaacg tgtgtgtaac tatatcagac
      121 ggcattgttt ttctgtttga cggcctcaat ccaaaatttt gccgacgatt tcgcccacgt
      181 ctttcgacaa tccttcctgc gcccgaatgc gctgcaatgc ttgtttcacc aagtttttgc
      241 ggtgcggctc gagcttgttg cagaggttga acgcctgcac caggcgggcg gcgacctgcg
      301 ggttaaagcg gtcgatttcg atgactttgt cggcgatgaa gcggtagccg ctgccgtctt
      361 ctgcgtggaa atgcgggacg ttgcggctga agctgccgat gagcgagcgg gctttgttgg
      421 gattttcgag gctgaacttc ggatgctgca aggcggtttg aacctgttgc agggtgtcgc
      481 tgcggcggct tgagccgacg agggcgaaat atttgtccat caccagcgcg tcgtctgaaa
      541 acttgtcggc aaactgagcc agcaggcggt tgcgcgtatc gctttcgttg ccgttaacgg
      601 cggacaggat gccccattcg tgggtcatgt tttgcgccat ttcgccgtat ttttcggcaa
      661 cggtttcgat gtgcgcgggg tcggcgcaca ggacgaaggc gcggcagacg ttgcgcagcg
      721 tgcgccagcc ggcagcttcg gggctgtact cgtagctttg gttttcctgc ttcgccgcct
      781 gacggttcaa ttcgtgccat ttcggcagga agcggacggc aagcgtatcc aacaaggctt
      841 cgcgcgcctg atggtagcgc agcgggtcga tgttttctgc gccgtcccac agttcggctt
      901 cagacggaac gcccaaaagc agggctttga aggcgttgtc caagaggtcg tctgaaatga
      961 ctttttcgac ggcggcaagc agtttttcat gtttcggcaa ctcgacgccg tctgaaagcg
     1021 cggcaaggtt ggcggcgacg gcgcggcggt agagcgtttg cgcggcttcc cagcgcgtga
     1081 aggcgtcgct gtcgtgggca agcaggagca gcaggtcatc gtcgctgtac ggatagttca
     1141 gatgcactgg cgcactgaac ccgcgcagca gcgagggaac gacggcttcg gttacgcctt
     1201 cgagcgggaa ggtctgttcg gcttcggcca gcagcaacac ggcttcggtc gcgcgtttgc
     1261 cctgataatc aaatgccacc gcttcgccgt tgcggttcag cagcccgatt ttgacgggaa
     1321 tcatcatcgg ctgcttgtcc gccatatcgg gcgtgggcgg cacggtttgt ttaatggtca
     1381 actcgaaaac attgtttttc agacgacctt ccgcttccaa aacgggcgtg cccgcctggc
     1441 tgtaccacaa ggcgaactgg tcgagattaa tgccgttcgc gtccgccatt gtcgcgcgga
     1501 aatcgtcgca ggtcacggct tgcccgtcgt ggcgttggaa atagagcttc atgccttttt
     1561 ggaagccctc ttcgccgagc agggtgtgat acatccgcac cacttccgcg cctttttcat
     1621 aaacggtcat ggtgtagaaa ttgttcatct cctcatagct ggcggggcgc accggatggg
     1681 cggtcgggcc tgtgtcttcg gggaactggt gctggcgcag caggcggatg ttctcgatgc
     1741 ggcgcacggc gcggctggcg cggtcgccgg aaaattcttg gtcgcggaac acggtcaacc
     1801 cttctttcag cgaaagctgg aaccaatcgc ggcaggtaac gcggttgcct gtccagttgt
     1861 ggaaatactc gtgtccgacc acggattcga tgccttcaaa atcggtatcg gtggcggtac
     1921 ggctgtcggc aaggacgaac ttggtattaa aaatgttcag ccccttgttt tccatcgcgc
     1981 ccatattgaa atcgcccacg gcgacgacca tgaaaatatc caagtcgtat tccaaaccga
     2041 agcgcgtttc gtcccacttc attgcgtttt tcagcgattc cacggcaaag ccgaccttgg
     2101 gcttgtccgc ttcggtggtg taaaactcga ttttgacgtt tctgccgctc atggtggtga
     2161 aatagtcttc cgttaccgcc aaatcgcccg cgaccaaagc aaacagatag ctcggtttgg
     2221 caaacgggtc ttcccatttc acccaatggc ggccgcctga atactcgccg ccgtcgattt
     2281 tgttgccgtt ggaaagcaaa acgggatagc gttttttgtc cgcaacgatg gtggttgtga
     2341 atttggacat cacatccgga cggtcgatgt agaacgtgat tttgcggaag ccctccggct
     2401 cgcactgggt aaatagattg ccgccggaag catacagccc catcagcgat ttgttttcca
     2461 ccggcaggat ttcggtttcc acttcaacgg tgaagcgttc ggatggcacg cctgcaatcg
     2521 tcagcgtctc gccttccaac acataatccg ccgccgcccc gttaattttg acggacaaga
     2581 gtttcgtcga accgtccaac accagcggct cccccaccct ctgcggctcg accgtcaaac
     2641 gggatttcac gacagtttgc ggttcggcaa tatcaaaatg caggtcggtt tcaaggatgc
     2701 ggtaggcagg cgtttggtaa tctttcagat aacggacggt tttgctcatt tttttctttc
     2761 aatattgttt ggtttgactg gaaaaggctt cagacagcac ggatacagct tttaaagcgg
     2821 attaacaaaa atcaggacaa ggcgacgaag ccacagacag tacagatagt acgggctgta
     2881 ccggtttgaa tttaatccac catatgccgt ctgaaaccga aacggcggca caagcgtacc
     2941 gccttcggac agcaggtttt ctatttccac gcttgggcaa attctgacca atgcggttta
     3001 tcttctgttt gggcggaaag atagtttctg atccgcttga tttccatttc gtattcgtcc
     3061 gcgtcgaatc tgccgctgac cagacaaaaa cgcagataca tcaaatacgt attcacggca
     3121 tcggtttcgc aataattgcg gatttctttc agcctgcccg cgtggaacgc atcccacacc
     3181 ttgctgccgt ccatacccag cttgcccgga aaaccgcaca gtttcgccat atcgtccagc
     3241 ggcacgtttg ccctcggctg gtaaagcgcg agcaaatcca tcaaatcgca gtggcgttgg
     3301 tgataacggc tgatgtagtt gttccacttg aaatcgcggc tgtcgccgaa atcgccgtcg
     3361 cccatatccc aatagcgcgc ggcgttaatg ccgtatatca gggagcggta atgcagaacc
     3421 ggcaggtcga aaccgccgcc gttccaactg accagttgcg gcgtatgttt ttcaatcaac
     3481 tcgaaaaatt tggcaatcac gacttcttcg ccatcgtcca tctcgccgat tgtaccgaca
     3541 tggactttat cctgccccca acgcatgcag cacgaaatcg ccacaatctg atgaagatga
     3601 tgctgcataa aatccccacc cgtctgagca cggcgttttt gctgggcaaa cagcaccact
     3661 tcatcgtcgg gcagcgagga cggcagctcg tacaatgttc ggataccctg cacatcgggt
     3721 acggtttcaa tatcgaaagc caaaatcgtg gtcatgacag caccttgtat ttaaaacaga
     3781 cttgcgccta ttgtgtcatt aaaaggcgga taaaaaaaga ggcaaccccc cacaggattg
     3841 ccccaatacc tcaaatcaga gatttacgct tcacaaacaa tacaggcttt cgcctgcggc
     3901 tttgcccgcg tagctcaact ctacgccggc aaacttttgt ttcaccgttt ccgatgaaac
     3961 cccgaccaat cgcaagactg accggaaaat cctttcagac ggcatttcct gcctgtcgtg
     4021 taattccatg tagcgaaatg tacgccattt tctacgcttt gccaagcatt ttttacaata
     4081 taaatgtcaa aacattaatt ttataaaatt gctgaaaata ttaaatatat ggatttttat
     4141 tttttatatt tcaataaata taaatttaat tttgatttat atttaaattt aagcataaaa
     4201 tgtcaaatat taaaggaaac atgaaaggca tatattaaat atttatttat aacgctatgt
     4261 ttttaaagaa aattaatttt aatatattaa ctagattgtc tgcatatatt cataggtttg
     4321 cggtatttct tccaaaacct gcttcgaatt tcccgaccaa gtcttaaaaa tattgttttt
     4381 tgagatactt aaatagcagc gattatcaaa tgaaatctgt tcatataatc tgccattttg
     4441 catttaaaaa aacaatcagg agtttcgact cgaaacgcct gatatgtttt gtaattttac
     4501 gtagtcagta aaaatcgggg ctgccttccg gacgggtttt aaaacgcttg tgcagccaaa
     4561 aatattgttc cggatgttcg cgcaccctgt cttcgataaa acggttcatg cgctgcgcgt
     4621 cggctttcgc gtcttcaccc ggaaaggatt tccaagcagg gtagaaatgc aatgtaaccg
     4681 tattgtctgc ctcgcggacg ggaatggcgg gtatcacttt tgcatttgca agcgcggcaa
     4741 tgcggctcaa tccggtaatc gttgccgtct gaataccgaa aaaatccaca aaaaccgaat
     4801 cgttgcgtcc gaaatcctga tcgggcagat acagaaacgg cgcgctgctt ttgcggaact
     4861 gtttgacgag ggcgcgcagc ccttcggtgc gcccgataag gaagacgttg tgatagcggt
     4921 tgcggccttt caaaatctgt tcgtccaata tcttgttttt ttgatgggaa tacatactga
     4981 tcagcgggat atcctgatta agcgcgtaca ccgccatctc gaacgcggtg aagtgcggat
     5041 acaggatgat gactttttcc cccgccgcca gcgcgtcgtc caaataatgc ttattgcggt
     5101 agcgcaccag cgatttcaaa cgtccggcag gcgcgtacca atataaaccg tattccaaca
     5161 tcagtttcgc catgtgtttg aaatgctgtt tcaacacggt tttacgcttt tcctcactcc
     5221 attcggaaaa acattttgcc aaattgattt cgccgatacg gcggcgcggt ttgaccagaa
     5281 ggtagacaag caaacccgtc aggtcggcaa tcttgtgcag cagcgcaaac ggcagaaact
     5341 gcaaaacata cagtacaaaa aatataaatt tcatctcgat acacattttc ttttcagacg
     5401 gcaaaataca aatgccgtct gaaactattg aaaccttccg cgcttgacct gcatccctga
     5461 aggattgagt ttggcggcaa gcccgtggtt gcgtaaggcg tgggtcagcg cgacggcaag
     5521 accgtccgcc gcatccgcct gcggtgttcc cgaaagcccc agcatctgca ccaccatatg
     5581 ctgcacctgt tctttcgctg ccttaccctt gccgaccacc gcctgtttga cctgcaaggc
     5641 cgtgtattcc gaaacgggca gcttatggct gaccaatgcc gccaatgccg cgccccgcgc
     5701 ctgaccgagc atcagcgtcg atgcaggatt gacgttgacg aacacctgtt ccaccgccgc
     5761 ctgttgaggc ttgtaaacgg taacgacttc gccgatgtgc cggacgatga cggcaatcct
     5821 gtctgccaga ggcgcatcgg caggcgtttt gatgcagcct gaggcaacgt aaaaatgatc
     5881 gcgccccctg acatcgatga tgccgaaacc cgttacgcgg ctgcccgggt caatgcccaa
     5941 gatacggacg gttgccgaca tattcacagc aaaccgtgtt gaatcagttt tttacgcaag
     6001 gtattgcggt tcagtcccaa catgacggag gctttggact gattaccgcc gcattgctcc
     6061 atcacgcaca ccagcagcgg cttttccacc tgatgcaata ccatatcgta cacgccgcaa
     6121 ggttcggtac cgttcaggtc tttgaaatat tgttctaaat tttgtctgat gcattgggaa
     6181 atatcgggaa gggtatgggg catgattgca ctttcaaagg ataatcaagt gttcagaagg
     6241 catttgggcg gtaggcgcac gcccaactgt cggttttttc ggcaagtctt tcaagataac
     6301 ctgcaagcat gtcgtattgc gccgccgcac tgtccaagcg gttgatttca cgacgtgtct
     6361 gttcgccgtc gggcatttcg tcgatgtacc agcctatgtg tttgcgtgcg atgcgcacac
     6421 cggcggtgtc gccgtaaaac gcgtgtatgg cgcggatgtg gttcaaaata gcggcggcgc
     6481 attctgccaa actcaaggca ggcggcaaaa caccgtgttc ggcataatgt ttcaaatcgc
     6541 ggaagaacca cggcctgcct tgcgcgccgc gccctatcat aatgccgtcg gcggcggttt
     6601 gtttgaggac ggcttgggct ttttgcggcg aagtaatgtc gccgttgacc cagaccggga
     6661 tgttcagacg gcatttggtt tcggcgatga gttcgtaacg cgcttcgcct ttgtacattt
     6721 gcgtacgcgt gcgtccgtgg acggcaaggg cggcgatgcc gcaatcttcg gcgattttgg
     6781 cgatgacggg caggttttga tggtcgtcgt gccaacccaa acgggttttg agggtaacgg
     6841 gtacgcctgc cgcacggacg acggcttcca aaatggcggc aaccagcggc tcgttctgca
     6901 tcagcgcgct accggcttgg acattgcaga cttttttagc gggacagccc atgttgatgt
     6961 cgataagctg cgccccaagg ctgacgttgt aacgcgcggc atccgccatc tgctgcggat
     7021 cgcttccggc aatctgcacg gcaacaatgc cgccttcatc ggcaaaatcg ctgcggtgca
     7081 aggtttttct agtatttctg agcgtcgggt cgctggtcag catttcgcac accgcccaac
     7141 ctgcgccaaa atctcggcaa agtcggcgga acggtttgtc ggtaatgccc gccatcggcg
     7201 caagtgcgat ggggttgtcg ataaaatagc cgccgatgtg cataatggat ccgcgtttca
     7261 aaaaagtacg ccattgtaca ttttttaagc aggatttcca atctccggac gcgcccgcga
     7321 ttgggtcgga caccgtttta tggcataatc cgcacacaga ttccctgccc cgccactcac
     7381 aggcgg
//
LOCUS       DAWISC010000104         7290 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWISC010000104 DAWISC010000000
VERSION     DAWISC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.104"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(128..940)
                     /locus_tag="WIW87_001457"
     CDS             complement(128..940)
                     /locus_tag="WIW87_001457"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ5174603.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1142..1603
                     /gene="tsaE"
                     /locus_tag="WIW87_001458"
     CDS             1142..1603
                     /gene="tsaE"
                     /locus_tag="WIW87_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ5174604.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            1600..2850
                     /locus_tag="WIW87_001459"
     CDS             1600..2850
                     /locus_tag="WIW87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5174605.1"
                     /translation="MIKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPQPGRGRNGRRPVIMLDPGHGGEDPGAISSGGLQEKHVVL
                     SIARETKNQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2908..4050)
                     /locus_tag="WIW87_001460"
     CDS             complement(2908..4050)
                     /locus_tag="WIW87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ5174606.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTASRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQTRPVRAPIAGSDNDRRIIQTALANARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(4099..4521)
                     /locus_tag="WIW87_001461"
     CDS             complement(4099..4521)
                     /locus_tag="WIW87_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174607.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(4514..5323)
                     /locus_tag="WIW87_001462"
     CDS             complement(4514..5323)
                     /locus_tag="WIW87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ5174608.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(5405..5782)
                     /locus_tag="WIW87_001463"
     CDS             complement(5405..5782)
                     /locus_tag="WIW87_001463"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ5174609.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(5982..6749)
                     /locus_tag="WIW87_001464"
     CDS             complement(5982..6749)
                     /locus_tag="WIW87_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5174610.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(6759..>7290)
                     /locus_tag="WIW87_001465"
     CDS             complement(6759..>7290)
                     /locus_tag="WIW87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ5174611.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLINVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1744 a         1994 c         1802 g         1750 t
ORIGIN      
        1 agaacgtaaa atctaaagaa accgttttat ccgataagtt tccgcaccga caggtctaga
       61 ttcccacttt cgtgggaatg acgggatttt aggtttctgt aatctcgggc agctttccgt
      121 catcgtttta acccaaagac accatttcaa tctgctccat cgtcctgccc agaaaacgct
      181 cgccgatggt tctgaatttc aaaggaatat cgctgacgta aaaacggtag tcgggattgt
      241 tgttgtcggt attgagcaat ccttcctgag caaggacgcg tgcggtttct tcggccgttg
      301 taattgcaga atcaaccaac gcgacattgc ccgcctccct gccgattaag ggcttgagca
      361 agggaaagtg cgtgcagccc aacaccagcg tatcgatgcc gtctgcaagc aatggtttga
      421 ggtattcgca tacggtcagg cgggtaactt cgtgttccag ccagccctct tccaccaaag
      481 ggacgagcag cggcgcggcc tgcgtgcgga cgagcgtgtc ggggttgttc ctatggatgg
      541 cgcgcgcata agcattgctg ttgactgtcg tattggtggc gataatgccg attttattgt
      601 tgcgcgtcgt tgccagcgcg gctttcgcgc cggcggaaat cacgtccaaa acgggcatat
      661 tgccggtttt ttgacggatt ttctgccccg ccaccgccgc aatcgtattg cacgcgataa
      721 ccatcgcctt gacatcgtgt tccaataaaa aatcgacaat ctgcatcgag aaattttcga
      781 tggtcgcctt agatttcgtc ccgtaaggca cgcgcgccgt gtcgccgaaa taaatgatgt
      841 tctccatcgg cagccgttcc atcagcgctc gcacattggt caaaccgccg attcccgagt
      901 caaaaacgcc gatgggtcgc tgcctgccga tattttccat tctttttcca atccgtccta
      961 aacatacaat ccgcgtattg tacacgcgcc gctttttctt gacagccgtt gccgtctgaa
     1021 agcagaacgc ggatttggct gttataatgc tcggacaaga agcatccgcc cctcgggtgc
     1081 aaagtttgca aaacctgcaa aactgcccat aataccctag accttacaac aaaccgtttc
     1141 catgtctgat ttaccctcta tctcccgatt ccttgccgac gaagccgcca cactcgattt
     1201 gggcgcggcg tggtcttccc gtttaaacgc accgctggtc atttatctcg aaggggattt
     1261 gggtgcgggc aaaaccacgc tgacacgcgg catcctgcgc ggattgggtc atcagggcgc
     1321 agtcaaaagt ccgacctacg ccatcgtcga atcttatccg ctggaacgct tcaccctgca
     1381 ccatttcgac ctctaccgct tctcgttccc cgaagaatgg gaagacgcgg ggcttgacga
     1441 actgtttgcc gcaaacagcg tctgcctgat cgaatggccg caacagggcg gggaatttac
     1501 gccgcccgcc gacatcaccg caacattgac acacgacggc gacggcagaa aatgcctgct
     1561 gaccgcccat accgaacgag gacgcgaaag cctgccgcta tgatcaaact gacacgaaga
     1621 caaatcatcc gccgcaccgc cggcacactg ttcgccctaa gccccatcgc atccgccgtt
     1681 gccaaaacgg tacgcgcccc gcaattcacc gccgcacgga tatggccgtc gcacacctac
     1741 acccgcctga cgctggaaag caccgccgcg ctcaaatacc agcacttcac gctcgacaac
     1801 ccgggcaggc tggtcgtcga catacaaaac gcgaacatca ataccgtatt gcacggactg
     1861 tctcagaaag tcatggcaga cgaccccttt atccgcagca tacgcgcggg tcagaacacg
     1921 ccgaccaccg tccgcctcgt catcgatttg aaacagccca cccacgcaca agtcttcgcg
     1981 cttccgcccg tcggcggctt taagaaccgc ctcgtcgtcg acctctatcc gcacgggatg
     2041 gatgccgacg atccgatgat ggcactgctc aacggtagcc tgaataaaac cctgcgcggc
     2101 tctcccgaag ccgacctcgc ccaaaacacc acgccccaac ccgggcgcgg cagaaacggg
     2161 cgcagacccg tcatcatgct cgatccggga cacggcggtg aagaccccgg tgccatcagt
     2221 tcgggcggcc tacaggaaaa acatgtcgtc ctatccattg ccagggaaac caaaaatcag
     2281 ttggaagcat taggttacaa tgtatttatg acgcgcaacg aagacgtgtt catccctttg
     2341 ggcgtgcgtg tcgccaaagg gcgcgcgcgg cgggcggacg tatttgtctc catccacgcc
     2401 gatgccttca ccagcccctc cgcgcgcgga accggggttt atatgttgaa caccaaaggc
     2461 gcaaccagct ccgccgccaa attcttggaa caaacccaaa ataatgccga cgcggtcggc
     2521 ggcgtaccga ccagcggcaa ccgcaatgtc gataccgccc tgctcgatat gacccaaacc
     2581 gccacgctgc gcgacagccg caaactcggc aaactggtgc ttgaagaatt gggcaggctc
     2641 aaccacttgc acaaaggcag ggtggacgaa gccaatttcg ccgttttgcg cgcacccgat
     2701 atgccgtcta tcctggtcga aaccgccttc ctgtccaatc ctgccgaaga gaagctgctg
     2761 ggcagcgaat ccttccgccg gcagtgcgcc caatccattg cctcaggtgt ccaacgctac
     2821 atcaatacat ccgtattgaa gcggggttga ttccgaaaaa taaatgccgt ctgaacattt
     2881 ttcagacggc atttttgtac actttcctca acggttcgat ccttccacca tatcaatcag
     2941 aaacaggcgg caatcgaggt tgccgttata aagtgggatt ttgcgttttg gcgacaggcg
     3001 catgaatttg ggcatttccc tgtcgccggt aaacataccg acccgccaac cggcatagtg
     3061 ttttttcaac cacgtcccca gctgcggata cagtgcctgc aaagcgtgga cttcctcaag
     3121 gcgcacaccg tagggcggat tagacaccat gatgccgttt tcgccgttcg gacggacgga
     3181 ttgcgcgtcg gcaacgctga aggaaacgat gtcgtccact tcggcactgc gtgcgttggc
     3241 caatgccgtc tgaatgatgc ggcggtcgtt gtcgctgcct gcaatcgggg cgcggacggg
     3301 gcgggtttgc gcttcggcgc ggcggcgcaa atctgtccac agcgttttat cgaaattttg
     3361 cagtttttca aaaccgaaac ggcgcatcat gcccggtgcg cggcctgtgg caatccaagc
     3421 agcttcgata gcaatcgtgc cgctgccgca aaacgggtct tgaaacggct gcgtgccgtc
     3481 gtagcctgcc gagagcagca gtcccgcggc aaggttttcg cgcagcgggg cttcgcccgt
     3541 atcgagacgg tagccgcgtt tgaacaaggc ttcgcccgaa gtgtcgatga agatttcgac
     3601 attgcgttcg tccaaaaagg cgtggatgcg gacatcgggc gcggctttgt ccacgctcgg
     3661 acgtgcgtcg taaatgtcgc gaaaagcgtc gcagacggca tctttgacgg tcaaaccgac
     3721 aaattggatg ctcttaacgt tggcacgctt tgcctcgact ttgactttga acgtctgctg
     3781 caaggtaaac caattaaacc agttgatgtt tttggcaagt ttgtaaatgt cgcgctcggt
     3841 tcggtatgtg cctttggtca gacgcagcag gatacggctg gcggtgcgcg agtgcaggtt
     3901 ggcggcgtag gcctgttcca atccgccccg gcaggaaacg ccgccgtcaa acgcttgaac
     3961 atcggtacag ccgaggcttc cgagttcttg agataaaacg gtttccaagc cgcgcgggca
     4021 ggtggcgaaa agtgtatata aagtattcat agcgtgtcct ttccggacgg atgttccggc
     4081 aatggcggat tgaaaggttt attcggcggt aaatgccgtc tgaaatgtcc gactgccgat
     4141 tgtaatgtcc gccgtaaaat cgcgcctgcc ttcgacacag acgggcaggc ggatgcgtac
     4201 tgcctgccaa gtccccgacg gttgccgctc gaacatatag cggttgaaac ccatatccat
     4261 atttttcata ctgaagctga tgctgacctg ttccgtgccg gcgggcgcgt gttcgatata
     4321 aatatcaaac ggttttttgg ttgaaacggc ggcggcgcgg acgcggcttc cgtccggcag
     4381 cgtgcaaccc tcggtcaaat cgcattgcgc cgccacagct tgcggctgct gcgcctgcca
     4441 ccattgcaac aaaacgagct tgacggcggc aaaggcaatc agcagcagtg cggcaagcag
     4501 taatttacgg tttttattca tgattcttcc ctattgtttc atcccttacc accacgtgcg
     4561 cccccaatcc gagccatagt tttccataac gtgcaaccgt ctgtccgttt gctgcaagta
     4621 cgagcggcac ggatacgccc tgccgcaggg cttctgccgc ctcttgtgcc tgaacatcat
     4681 cgcacacgcg ccaaacgacc gcatccgcgc ccttttcctg cgcccgccgc cattgttcgc
     4741 gggtctgcac caccatccac acgctgtcgg cgtgttcggg tctgtcctgc cattgcgccg
     4801 cctccatcaa aacgttcgca ccgcttccct ctgccgaacc caaaggcagg acgcggtacg
     4861 cgcccatact gttttctccg tgcaaacccg ttttcaggct gccgtacaaa cggcgcggga
     4921 cggacagcga acgcaaaagc gcgccgttgg cgggcagcat gggggcaacg gtaaaatcac
     4981 ccgccttctg ccaagaccat tcctgccctt cgcgggattg cggtttgccc gtccattggt
     5041 cggggttgac ccataggaat ttcaggcaga cgcgggcgtg ttcgtaggaa tggattttgg
     5101 tcaaccaagg cgtggcggcg aggatgcgga tgccgagttc ttcttcaaac tcgcgttgca
     5161 gggcttggaa gtcggtttcg cccgcttcga ccttgccgcc ggcaaattcc caatatccgg
     5221 catagggttt gccttcgggg cgcgagctga gcaggtagtt gccgtctgaa tcgagcagga
     5281 tgccggcaac gacgcggata aggggtcggg tgtcttgaat catggcgggt acgcggttgg
     5341 tcggtcggag tgcaaatgag ggacgggcaa tgccgtcaac gggtttgccg catttcagac
     5401 ggcattattt ttcggcaacg acaaacgcca atacggtgtc ttcttcgtcg ctcatgctga
     5461 ggctgacgcg gctgatgcct tgttcctcca gccatttgga cagggcgggg ccgtagaaaa
     5521 attcgggctt gcccaatgcg tcatgcccga tgccgatgtt gcggaaggaa accgcgccgc
     5581 gtatgcccgt gccgacggct ttggcaaagg cttctttggc ggcaaagcgt ttggcgaggt
     5641 agttgacggg tttgcccgct tgcggaaatt caagcagctc ttccggagtg aggatgcgcc
     5701 cggcaaacgc ctgtccgaat tttttgttta agcggatgat gcgcttgagg gaaacaatgt
     5761 ctgtgccgat gccgtaaatc atatttgcgc tccttcgccc ttggttgcgg gtagtgatga
     5821 atgtgatgat gaatgccgtc aatttcttgg ggcgttaaaa tggcgtgaat atccactttt
     5881 ggggttttgt cggtaacaat tttcactgta accttttgca tttgaaattc taaaacatcg
     5941 tgccaattat ttaataagga gttaaggcat ttaataatga attaaagttt gattaaaccc
     6001 gaatgcccga ttttcagagc caataccaat aaaacaataa aaattaacat tttaaaaaaa
     6061 tttgggctaa tcttagtcct taaccgaatt ccaacataca atccaataac agacaatacg
     6121 gacagtaaaa atattaaacc gtattcactc ttatttaata accaatactg gtctcttagc
     6181 atatatattt gaacaatttt cgccaaaaga tagcatagat tgcttgattt tacgatacga
     6241 tttttatttt ctgtttcgct aagcaaaaat attaacaata tgggagacat ggcattggtt
     6301 gaaccgccga tgatgcctgc caaaaaccca aaaagaacca tattcttatt attggcaact
     6361 acttgaatat tttttgcttt tgcacataca tttaaaatac cattgacaga ataatacaat
     6421 gtaatgattg ccatcagtaa aagcagccaa gacactggaa gtatcaaaag caacttcacc
     6481 cccaaaatgc tgccaacgac gctgccgata gcaagcaatt tataggtttt taaataataa
     6541 acaatctctt gccaaaaacc ctttttgtta ttgctgcata gaaccaacaa gctcattaac
     6601 aggcttggta atgccaccaa ggcaacaacc ttagacaatg gcatgataaa agccaatgcg
     6661 gttgtaccga gcatcggaaa tcccatgcct gtaattccgt gcagtattgc ggcagcaaca
     6721 aaaacgatag attgcattat ttcttgcata accgtccttt atacagtaat tcttgctcta
     6781 atataatgcc tgtttttatc cgtataatca gttctggcat gcaagccatt gacattgtca
     6841 ataataatca aaccatcatc ttgtgcagaa aattcttgaa tatcactcgc attttttata
     6901 acatttataa gataatcaag tgcaagtacc atttcctttg atactttatt tctattttct
     6961 tcaatacctt tatagataca atcacttcta aatctaatca tttgagtatt gaccgataag
     7021 atattacccc atctcacacc ttgtttttta tcaaatgatg ctggtgtttt aaatggatat
     7081 aaattgcccg ttaatgtttt taagtgtttt ttaccggttt ctgttttaga taactgattg
     7141 acaatatctg atgaacttaa aaatagggaa ttacctccat cattggctga ttttactaca
     7201 tacataacca aataactttc cgggttttca ctaaaagatg aatcactatg aagtggacat
     7261 tctccaacat cttccgagaa agttatatca
//
LOCUS       DAWISC010000105         7248 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWISC010000105 DAWISC010000000
VERSION     DAWISC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.105"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..164)
                     /locus_tag="WIW87_001466"
     CDS             complement(<1..164)
                     /locus_tag="WIW87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEZ5174612.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIE"
     gene            403..1302
                     /gene="crgA"
                     /locus_tag="WIW87_001467"
     CDS             403..1302
                     /gene="crgA"
                     /locus_tag="WIW87_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ5174613.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIVCLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WIW87_001468"
     CDS             complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WIW87_001468"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ5174614.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQVVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4771..5412)
                     /locus_tag="WIW87_001469"
     CDS             complement(4771..5412)
                     /locus_tag="WIW87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174615.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6094..6483)
                     /locus_tag="WIW87_001470"
     CDS             complement(6094..6483)
                     /locus_tag="WIW87_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ5174616.1"
                     /translation="MSTHEKLLTADNPVLHQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(6924..>7248)
                     /locus_tag="WIW87_001471"
     CDS             complement(6924..>7248)
                     /locus_tag="WIW87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEZ5174617.1"
                     /translation="VNSLYTQVLGMEEVSFGSDRKALLFGSQKINLHGRGAEIQPNAQ
                     HAACGTADLCLLTDTPLETVLQELSAHGIKPLSGIVARTGAMGKIQSVYLRDPDGNLL
                     EISSY"
BASE COUNT         1634 a         1858 c         1852 g         1904 t
ORIGIN      
        1 ttttcgattt ctgcctcaac atcatagccg gcatcaatca cggtttcttg aacattgtgt
       61 ccgagcgcgg tcaaaacttc tttcgctttt ttgtgaagcg tacggtttaa cccgccgtga
      121 gaatgtccga acgccttgcc gccgtctaat aataaaatat tcatttgtta cctcgtttgt
      181 gaattgatga atgtattgtg ccgttttact ttataattta aaagtgcaaa aataagaaaa
      241 cacttttgcg tcaaatgaaa taatcagatg ttccaacaca cgggatggca cataaagcac
      301 cgccctgtgt gccgtcctga ttcggaaggg gttacacccc tcccaaataa agtctgatcc
      361 tgccgcccta aagggcgggg ttccaaccga aaaggaaaca cgatgaaaac caattcagaa
      421 gaactgaccg tatttgttca agtggtggaa agcggcagct tcagccgtgc ggcggagcag
      481 ttggcgatgg caaattctgc cgtaagccgc atcgtcaaac ggctggagga aaagttgggt
      541 gtgaacctgc tcaaccgcac cacgcggcaa ctcagtctga cggaagaagg cgcgcaatat
      601 ttccgccgcg cgcagagaat cctgcaagaa atggcagcgg cggaaaccga aatgctggca
      661 gtgcacgaaa taccgcaagg cgtgttgagc gtggattccg cgatgccgat ggtgctgcat
      721 ctgctggcgc cgctggcagc aaaattcaac gaacgctatc cgcatatccg actttcgctc
      781 gtttcttccg aaggctatat caatctgatt gaacgcaaag tcgatattgc cttacgggcc
      841 ggagaattgg acgattccgg gctgcgtgca cgccatctgt ttgacagccg cttccgcgta
      901 atcgccagtc ctgaatacct ggcaaaacac ggcacgccgc aatctacaga agagcttgcc
      961 ggccaccaat gtttaggctt caccgaaccc ggttctctaa atacatgggc ggttttagat
     1021 gcgcagggaa atccctataa gatttcaccg cactttaccg ccagcagcgg tgaaatctta
     1081 cgctcgttgt gcctttcagg ttgcggtatt gtttgcttat cagatttttt ggttgacaac
     1141 gacatcgctg aaggaaagtt aattcccctg ctcgccgaac aaacctccga taaaacacac
     1201 ccctttaatg ctgtttatta cagcgataaa gccgtcaatc tccgcttacg cgtatttttg
     1261 gattttttag tggaggaact gggaaacaat ctctgtggat aattctataa aaacctgtca
     1321 taaaactgtt tttactcact taataacttt gagaaaaaat aaataagata atgtatagtc
     1381 ggcggcaatg gcaatctata tggggagcgt gatagggctg ttccttatgt acggcttgtg
     1441 tgtggcaaca tcggctttcc aaatgacaaa atgccgtctg aaaccgcaaa acggcttcag
     1501 acggcatttt caacctgatt caggcatcag ttgcggtttt tcaaacgccc gtgcagttct
     1561 tgaacgctgt acacgcccag atggtctcgg cttttcgcgc cttcgctcat cgcttcgccg
     1621 ccggcggtgg tggtgtattg cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg
     1681 ctgtcggaca cggattgcgg atcgctggaa acggtgttca cgaccagtgc gatttcgccg
     1741 tttttcagcg cgtcgccgat gtgcgggcgg ccttcgggta ctttgttgat ggtctgcaca
     1801 atcagcccgt gttcggtcag gtattgcgcc gtgccgcgcg tggcgcagat gccgtagcct
     1861 aaaacttgga agtttttagc ggttttaatg acgcgttctt tgtcttcttc gcgcacggag
     1921 aggaagattt tgccggtcgg gttgaggcgt tcgcccgcgc cgagttgggc tttgtagtag
     1981 gcttcgccaa agcttgcgcc cacgcccatg acttcgccgg tggagcgcat ttccggtccc
     2041 aaaatcgtat ccacgcccgg gaatttgatg aatgggaaca cggcttcttt aacggcatag
     2101 aaatcgggga caacttcttt ttccacgcct tgttctttca gggaaatgcc tgccatgcag
     2161 cgcgcgccga ctttggcgag cggcacgccg gtggctttgg agacgaaggg cacggtgcgg
     2221 ctggcgcgcg ggttcacttc caatacgaac actacgccgt cttgtacggc aaactgcacg
     2281 ttcatcagtc cgaccacgcc cagcgcgtac gccatcgctt tggtttggcg gcggatttcg
     2341 tcttggattt cttcgcttaa ggagtagggc ggcagcgagc agccggagtc gccggagtgg
     2401 atgcccgcct gttcgacgtg ctgcatgatg ccgccgataa ccacgtcttt gccgtctgaa
     2461 acgcagtcca catccacttc aatcgcgttg ttcaggaaga agtcgagcaa cacggggctg
     2521 tcttcggaaa cctgcacggc ttcgcgcatg tatttttgca gctcttcggc ggaatggacg
     2581 acctgcatgg cgcggccgcc gaggacgtaa gacgggcgca cgaccagcgg atagccgatt
     2641 tcttcggctt tgacgagcgc ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc
     2701 aggcctaagt cgttcaacac tttttggaag cgttcgcggt cttcggcggc gtcgatgctg
     2761 tcggcggacg tgccgatgat gttcacgccg ttttcaacca gcgcgttggc gagtttgagc
     2821 ggggtttggc cgccgtaatg cacaatcacg ccccacgggt tttcggtgcg gacgatttcc
     2881 aacacgtctt ccagcgtcag cggctcgaaa tacaggcggt cgctggtgtc gaagtcggtg
     2941 gacacagttt cggggttgca gttgaccatg atggtttcaa agcccgattc gcgcagggcg
     3001 agcgcggcgt gaacgcagca gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg
     3061 ccaccgagaa tcatcacttt tttgcggtcg gaaggacgag attcgcattc ttcttcgtaa
     3121 gtggagtaaa gataggcggt ttcggtggcg aactcggcgg cgcaggtatc gacgcgtttg
     3181 taaaccggat gcagcttcag cgcgtagcgg tgttcgcgaa cttctttttc gcttacgttc
     3241 aacaattgtg ccaaacgttt gtcggagaag cctttgcgtt tcagacgacg tagggcggcg
     3301 aaatccaaat cactcaaaat gccgtctgaa accgcttttt cttccttcat caagtcttcg
     3361 atttgcgcca agaaccaagg gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag
     3421 cccgcgcgga acgcgtctgc cacaaacagc atacgttcgg ggccggggtt cgccagttcg
     3481 cggcggattt ccgctttgtc ttcactgcgc ggattgaagc cgcacaagcc tgtttccaag
     3541 ccgcgcaggg ctttttggaa actttcttga atcgtgcggc ccatcgccat cacttcgccc
     3601 accgatttca tctgcgtggt caggcggtcg tctgcggcag ggaatttttc aaacgcgaaa
     3661 cgcgggattt tggtaaccac atagtcgatg gaaggctcga acgacgcggg ggttttgccg
     3721 ccggtgatgt cgttgcgcaa ctcgtccagc gtaaagccga ccgccagctt cgccgccacc
     3781 ttcgcaatcg ggaaacccgt tgctttggaa gccaacgcgg aagaacggct cacgcgcggg
     3841 ttcatctcaa tcacaatcat ctcgccgttt gcagggttca ccgcaaactg cacgttcgag
     3901 ccgcccgtgt ccacgccgat ttcgcgcaat accgccagcg aagcattacg cataatttga
     3961 tattccttgt ccgtcagcgt ttgcgccggc gcaaccgtaa tcgagtcgcc tgtatgcacg
     4021 cccatcgggt cgaagttttc aatcgagcag atgatgatgc agttgtcgtt cttatcgcgc
     4081 accacttcca tctcgtactc tttccagccg agaacggact gctcaatcaa cagctcgtgc
     4141 gtgggcgacg catcgaaacc gcgttcgcaa atcgccaaaa actcgtcttt attgtaggca
     4201 atgccgccgc ccgaaccgcc catggtgaaa gaaggacgaa tcagggtagg gaagccgacc
     4261 tgttcttgcg ccgccaaagc ttcgttcatc gtgtggcaga caaaagattt cgggcaggag
     4321 aggccgattt tctccatcgc ctccttaaag cggccacggt cttctgcttt gtcgatggcg
     4381 tcttcggtcg cgccgatcag ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa
     4441 tccagcgcac agttcagcgc agtctgacca cccatggtag gcagaatcgc gtcaggacgc
     4501 tctttggcaa taattttttc caccgtctgc cacataatcg gctcgatgta ggtaacatcc
     4561 gccatttcgg ggtcggtcat aatcgtggcg gggttggaat tcaccaaaat gactttatag
     4621 ccttcttcac gcaaggcttt gcaggcctgt gcgcccgaat agtcaaattc gcaggcctga
     4681 ccgataacga tagggccggc gccgatgata aggatggatt ttaggtcggt acgtttgggc
     4741 atggtgggtc tttcttttga agaatttgga ttattgacga ttggtaactt ctctaattat
     4801 ttctgcatct gttttttcat cgcttggatt atctgaaaag aaaaaactgt aattactatc
     4861 ggctttaaat ttttcgtata gctctgggcg caaaattttt gctttttcac tattttccac
     4921 gctcctgcga ttaggcttgc cagccttatc tttcccattt ctacggacat catagcaata
     4981 aatagtgtta ggagaattag gttctaaacg agtagctaca atctttttat aaattatgtc
     5041 taaatctttt ttagtttgtg ttggaatgaa atctgcaaaa agattactaa aactctgaaa
     5101 aatttctctg attttcgcag caccagcctg aacattaatt ttaaaaaaat taataaaatc
     5161 agtagcactt ttaaaatgaa cttcataaaa atcagcatac gcaaatattg aattttcact
     5221 attcaaatta aaaaactcta attttgcttg tgcataatca tctggtctag gttcatcagg
     5281 tttataccta gtcaaaataa cggcgtattg cctttgatta taattaaaag gaaatgcttc
     5341 aattattcta tttttgtttc tcatatcatt aagcaaatac tttaaattat tgaaattaat
     5401 aggtattgac atatttaaac tcctgcgaat atgttgcaat aaacgtagca agcgtaggtt
     5461 gggttgaaaa cccaacacaa accgcttaat tgtctaaaat gttgggtttg ctaaaaccta
     5521 acttgtatat cgaaaccccc gtatcgtagc aagccgtagc ccgtatgtag ggtatgtgcg
     5581 gtacgtatgc acgcgttctc cattttccct gcaaccccaa cccaccgcgt gcgtgccttg
     5641 cggcacactc tctgccgatg agttcaaagg tcgtctgaaa aaacgttcgc ggcgtttgtt
     5701 ccacctgcct tgcgataaag gctgcctgaa aaattcccgc tgttgatatt tcgggcattc
     5761 tttctcaaac cgcctgcaac cgcaaaatcc gctgcaacct gccccctccc ccgtggggga
     5821 gggctgggga gatggaattc tccgaatttc ggcaaccttc cccaatgcct tcaccgccta
     5881 aatacaagcc ttgcggcttg ttgccctctc tctaacgctt tcccacaggg agagaggacg
     5941 gggcggctgt tggaattaag ggttctgcag gaaaaacagg ttgtgcgggc tgttttttgt
     6001 ttttcagacg gtcttctaaa cgctcaattc aagcagcctg taccctacct gtatcctata
     6061 attttcaaaa ctctaatcaa aattaaccgt ttgttattgc gcatactgct tttccaattc
     6121 ctgcaatacg cgcaggattt ccgccagtac atcgtttgtc tgctgcaaaa tttcgtgatt
     6181 ccaaaaacgc agcacggtaa agcccaggct gttgagatat gccgtccgcg cgtggtcgta
     6241 tacggcttgt tccgcgtgct gcccgccgtc tgcttcgaca atcagcttgg gcgttacgca
     6301 cataaaatca acaatataat tccccatcgg ctgctggcgg cggaatttat agccgttcag
     6361 acggcctgcc cgcaggtgct gccacaattt tgcttccgcc tcgctcattt cttggcgcat
     6421 ggctttggcg cgttgatgca ggacggggtt gtcggcggtt aaaagtttct cgtgcgtgct
     6481 cattctgttg tcgggttact gtttgtgtaa gccttacagt ttgttgccct ctctctgact
     6541 ctctcccatg ggagagagga cgtggtggcg gttgggatta agggttttgc aaggaaaaca
     6601 ggttgtgcag gctgcttttt atttttcaga tgacctgaaa caacagctgc aaatctaagt
     6661 tgcctttcaa accgcctgca accgcaaaat ctgctgcaac cttctccctc ccccgtgggg
     6721 gagggccggg gagagggcat tccccgaatt tcggcaacct tccccaacac cctaaccgcc
     6781 caaacacaag ccttgcggct tgttgccctc tctctaacgc tttcccacag ggagagagga
     6841 cggggcggct gttggggtta aagaggctgc aaggaaaaca gcttgtgcag gctgtttttt
     6901 attttgcata agccgtctga aaatcaataa ctgctgattt ccagcaggtt gccatcggga
     6961 tcgcgcaggt aaaccgattg gattttgccc attgcgcctg tgcgcgctac gatgccgctt
     7021 aaaggtttga tgccgtgtgc ggataattcc tgtaaaaccg tttccagtgg cgtatcggtc
     7081 agcaggcata aatccgctgt gccgcaggcg gcgtgttgcg cgttaggctg aatttccgca
     7141 ccgcgcccgt gtaggttgat tttctgactg ccaaacaaca aagctttacg gtcgctgcca
     7201 aatgaaactt cttccatgcc caaaacttgt gtgtatagtg aattaaca
//
LOCUS       DAWISC010000106         7239 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWISC010000106 DAWISC010000000
VERSION     DAWISC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.106"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(76..831)
                     /locus_tag="WIW87_001472"
     CDS             complement(76..831)
                     /locus_tag="WIW87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ5174618.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(956..2128)
                     /gene="nirK"
                     /locus_tag="WIW87_001473"
     CDS             complement(956..2128)
                     /gene="nirK"
                     /locus_tag="WIW87_001473"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ5174619.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAAAAEAASSAAQ
                     TAAETPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASASEKSVY"
     gene            2499..4754
                     /locus_tag="WIW87_001474"
     CDS             2499..4754
                     /locus_tag="WIW87_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ5174620.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSVLGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4933..5466
                     /locus_tag="WIW87_001475"
     CDS             4933..5466
                     /locus_tag="WIW87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ5174621.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5529..6716)
                     /locus_tag="WIW87_001476"
     CDS             complement(5529..6716)
                     /locus_tag="WIW87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ5174622.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVYPKPPRHSNSADNLLRIVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVAD
                     ISE"
     gene            complement(6756..7160)
                     /locus_tag="WIW87_001477"
     CDS             complement(6756..7160)
                     /locus_tag="WIW87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ5174623.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPNLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1618 a         1750 c         2025 g         1846 t
ORIGIN      
        1 ggattaacaa aaaccagtac ggcaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
      121 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      181 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      241 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      301 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      361 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      421 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      481 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      541 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      601 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      661 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      721 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      781 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      841 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccaccgcc
      901 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      961 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
     1021 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1081 tttctgcacc ttctactttc aattgaccca gtgcgccttt gttgaatgcg cggaagatag
     1141 agtggtcaac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcagagc
     1201 cgccggcagg aacgatggtg ctttgtacgt tttcgttaat cagtttgccg ccttcaacat
     1261 aaactttgtc gaagatttcg ccgatgacgt ggaaggaaga taccaagttc ggaccgccgt
     1321 taccaacgta catacgtaca gtttcgcctg ctttggcttt cagcgcgtta tcgccggcga
     1381 tagcacctac gtgaccgttg aatacgacgt attcaggctg ttcggcaacg gctttgtcca
     1441 tatcgaacgg ttgcagacct tgcgcgcctt ttttgccttt ggtgtagaag tcgccttgga
     1501 cgatgtagaa ctctttatcc actttcggca ggccttcttt aggctcgacc aaaatcagac
     1561 cgtacatacc gttggcgatg tgcataccga ccggtgcgac ggcgcagtgg tagatgtaca
     1621 gacccggttg cagggctttg aagctgaatg tggaagtacg gcccggagcg gtaaaggttg
     1681 cggccgcgcc gccgccctgg ccggtagccg cgtggaagtc gacgttgtgc ggaacggtag
     1741 aagaaggatt gttggaaaat tccacttcaa ccgtatcgcc ttcgcgtacg cggatcatac
     1801 ggcccggaac gtcgccgtca aatgtccagt agcggtattc cacaccgtct tccatggtca
     1861 tggttttttc gacggtttcc atttttacgc ggactttggc ggggtagtcg cggtcgattg
     1921 caggaggcac ttcgggagcg tgggtggtaa ccgcatcgat aacgggcagt tcgccggacg
     1981 gtgtttcggc ggcggtttgt gcggcggagc ttgcggcttc ggcggcagcg gcaggggttt
     2041 cggcaggggc ttgcgcggca ggttcgccgc cgcaggcggc taatgcgaat aaggaagcaa
     2101 tcattgcagc taaggcttgg cgtttcataa tattttcctt ttgtaagaaa agtagaggga
     2161 ttggttaaga acttggtgtg attatgcgtt tgccccattg ggggcatttt gatttaagtc
     2221 aaataattca taaaatatga acttaaaatg atacttctaa tactttatag ctactaaaaa
     2281 ttacaataaa taggctgatt tgcttaaggg cttcggcaaa tcaggcaaag gcagggtagt
     2341 tgccgactaa agaataatga tagttattat catatatgta tttgttttat atgtgagttt
     2401 ctttgaacaa atgtttattt gcgcggtaaa ccgtgctaca atctttaaca ttcatatttt
     2461 gtgaatttta atccactata ttaaaaggag ctctcaaaat gggacagtac aagaagcttt
     2521 ggtacttgct gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg
     2581 aggtttacaa aaaagccccg ccttaccccg aacaggtcgt ttccgcatcc ggcaaagtct
     2641 tgatgacgaa agacgatatt ttggcaggtc agtctgcatg gcagactacc ggcggtatgg
     2701 aagtcggctc tgtattgggt cacggtgcat accaagctcc ggactggact gccgactggc
     2761 tgcatcgtga gttgtccgcc tggttggatt tgaccgcgca acagacttac ggcaaaaaat
     2821 tcgatgaagt ttcccctgaa gaacaagccg ttctgaaaac ccgactggct gacgaatacc
     2881 gcaaccaaag ccgtattaaa gaagacggca gcgtcgtcat cagcgatacg cgcgtgaaag
     2941 ccatcgaaag catcctgcct tattatcacg gtgtttacgg cgatgatccc gcgctgcaaa
     3001 ccacccgcga acactttgca atgaaaaaca acacattgcc aagccaagaa gcgcgtgaaa
     3061 aactgttcga cttcttcttc tggacttctt ggtctgcttc gaccaaccgt cctgacgaga
     3121 cgttcactta taccaacaac tggccgcacg agcctttgat caacaacgta ccgaccactg
     3181 aaaactatat gtggtcgttt accagtgttg tactgctctt gatgggcatc ggtttgttga
     3241 tgtggggtta ttccttcctg accaaacacg aggaagtgga agtgccgtct gaagacccga
     3301 tttccaaaat ccaactgacc ccttcgcaaa aagcattggg caaatacgtc ttcctgacgg
     3361 ttgccctgtt tgtggtacaa gtattgctgg gcggcctgac cgcgcactac accgttgaag
     3421 gtcagggttt ctacggcatc gatgaggcgt tgggctttga aatgtccgac tggtttccct
     3481 atgccctgac ccgtacttgg cacatccaat ccgccatctt ttggattgca accggctttt
     3541 tgacggcagg tttgttcctc gcgccgattg tcaacggcgg caaagatccc aagttccaac
     3601 gtgcaggcgt gaacttcctc tatatcgccc tcttcatcgt ggtcggcggt tcttacgcgg
     3661 gcaacttctt cgcgttgacg cacatccttc cccccgaatt gaatttctgg ttcggacacc
     3721 aaggttacga atacctcgat ttgggacgtt tctggcaact ccttttgatg gtcggtctgc
     3781 tgttgtggct gttcctgatg ctgcgttgca ccatctccgc ctttaaagaa aaaggcgtgg
     3841 acaaaaacct gctggcaatc tttgtcgctt ctatggtcgg tgtgggcgta ttctacgcac
     3901 caggcctgtt ctatggcgaa aaatccccga ttgccgtaat ggaatactgg cgttggtggg
     3961 tggttcacct gtgggtggaa ggcttctttg aagtatttgc cactgccgct tttgcctttg
     4021 tcttctacaa tatgggcttt gtccgccgca gtaccgccac agcttctact ctggccgctg
     4081 ccgccatctt tatgttgggc ggcgttccgg gtacgctgca ccacctgtac ttctccggct
     4141 ctacctccgc ctctatggca atcggtgcct gcttctccgc tttggaagtc gtgccgctgg
     4201 tgttgctggg ccgtgaagca tacgagcatt ggtcttacca acacctgtcc gactgggcga
     4261 aacgcctgcg ttggccgctg atgtgcttcg tggcagtcgc cttttggaat atgatcggtg
     4321 ccggtgtatt cggtttcctg attaacccgc cgatttccct gttctatatc caaggtttga
     4381 atacttctgc tgttcatgcg cacgccgcct tgttcggtgt gtacggtttc ttggcattgg
     4441 gcttcgtatt gctggttgcc cgttatttga aaccgaacgc gcagtttgac gacaaactga
     4501 tgacttgggg cttctggctg ctcaacggcg gcttggtcgg tatgattgcc atcagcctgt
     4561 tgccggttgg cgtgattcag gcatacgcct ccatcacgca cggcctgtgg tatgcacgca
     4621 gcgaagagtt cctgcaaatg gaaatcctcg acaccttgcg ttgggtacgt acggcagctg
     4681 acttggtctt tatcggcggt gcaatctgcg ttgccatcca agccactaaa atcgtattcg
     4741 gccgcgacaa ataagttttg cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt
     4801 tttttattgc ggattgggac gggaatggaa aaggatgtgg attttttatt ttaatcggct
     4861 atacaattgc tgctttcatg ccgtctgaag cacgtttcag atggcatcgg tttacatcag
     4921 ataaggataa aaatgggtat ttacgatttt caaatgaaag atgcggaagg caatgcggtt
     4981 gatttgtcag gctatcgcgg caaggttctg ctgattgtca acacggcaac gcgttgcggt
     5041 ctgaccccgc aatacgaggc tttgcagaag ctgtatgcac aatataccgc agaaggcttg
     5101 gagattttgg attttccgtg caaccagttc cgcgaacagg ctcccgaaag cagcggagaa
     5161 attgcccaag tgtgtatgat gaagtttggc acgaagttta agattttcga caaaatcgaa
     5221 gtcaatggag caaataccgc gcccttgtat gcctacctga aatccgtcaa accgcaagat
     5281 aaaggcaatc atctgtttaa agatttcgtg ttgaagctcg cagctttggg agagaagcgc
     5341 gacgaaggcg acatcaaatg gaactttacc aaatttctcg tcaaccgcga cggcgaagtg
     5401 gtcgagcgtt ttgcgcctag tgttacgccc gaagaaatcg aagccgatat tcgggcattg
     5461 ctgtaatctg ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt
     5521 ttgcgggttt attccgaaat atcggcaaca ccgctttcgg ggaagatggg caaatccggc
     5581 ggtgggagca gggtatccaa agtcagggcg gacgcatcga gtgtcgggta gccttcaaac
     5641 gcgaccgaat aaccgctgtt tccgttcata cggattcggg cgtgcgcgcc tagagggaaa
     5701 gtgattttgt cggcaatgtg tccgaacggg aagcccgtca gcacggggat tttcgccgtg
     5761 cgcgaaacat ggttggcaac ggcagaaaaa tcataagacg gatcatagac atctcgaatt
     5821 ttttccatac ggaaattgcc gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga
     5881 tacagcgtat tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac
     5941 aaaatgccgc cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc gaggacgctt
     6001 aagttgccgc cccacaatat gccttcggtt tcgacatcgg cgcgttggat ataaggaaca
     6061 tcaacggtca ggcggttttg ggttgcaccc ttgataaagg catccatcgt aaacgcaccg
     6121 ggggcgggtt tgccaaaatc gctataagcc atcgggccgg caaaactcat catattgcct
     6181 tttgccaaca atgccagctg gacggcgcat acgtcgctga atccgaaaaa cagcgtgccg
     6241 tgttcgcgca tccttgcgcc gagcgaagca aaatcgatat gcggcagaat gcgtgccgca
     6301 ccataaccgc cgcgcaagcc catcagcact ttaggtgtgg caacgcgacc ggaagcgaca
     6361 tcttggaaat cggcggcacg ttgtgagtcc gtgccggcaa accgttggaa acggcggctg
     6421 cccgcctgtt ggttggttac ggtaaaaccg gcattgtaaa ggcgggttaa ggctgtgttg
     6481 acgcggttgg tgtcttcggc aaatcctgac gaagcgacaa tacgcagaag attgtcggca
     6541 gagttggaat ggcgtggtgg tttggggtac acggttcggg ctttcgcaac aggatgagag
     6601 ggcgcggatg gggcattgtt tgaagcaggc gcagtaccgc aagcctgaag cagccccgcg
     6661 ccggcggcgg cggtgcaggt tttcaggaaa cggcggcggg aggtcggttc ggtcataatt
     6721 tccctttcat acggtttcag acggcattgg cgggcttaaa gcagcttttt aagctgttcc
     6781 gtccaatctt gcggcagggt cataccgtgt tttcggacgg ggtttgccca aatcggcgcg
     6841 gggaaggacg catcgttttc aaagcgggcg ataatatgcc agtgcaggtg cggcacgaca
     6901 ttgcccaagc tggcgaggtt gatttttgcc ggccggaaca cttggcgcat agcggcttcg
     6961 actttgtaca ccatttccat caattcgccg cgttccgctg ccgaaaggtc ggtcatttcg
     7021 gcaatatgct tacgccaaat gacgcggcag aatgcaggcg aaccgctgtc gttatggacg
     7081 gcgatgacgc ggaggttggg ggtttgcagc aaaacgtctt cattttgcgc cgtgcagatg
     7141 gggcagggtt gggcggtcat ttttccttat tcctgaaagt gtttaaaaaa cggtagggta
     7201 atttctgata tagcggatta acaaaaatca ggacaaggc
//
LOCUS       DAWISC010000107         7204 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWISC010000107 DAWISC010000000
VERSION     DAWISC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.107"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(64..148)
                     /locus_tag="WIW87_001478"
     tRNA            complement(64..148)
                     /locus_tag="WIW87_001478"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="WIW87_001479"
     CDS             425..1078
                     /locus_tag="WIW87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ5174624.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /locus_tag="WIW87_001480"
     CDS             1157..1810
                     /locus_tag="WIW87_001480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ5174625.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="WIW87_001481"
     CDS             1914..2813
                     /locus_tag="WIW87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ5174626.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="WIW87_001482"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="WIW87_001482"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ5174627.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /locus_tag="WIW87_001483"
     CDS             4145..5242
                     /locus_tag="WIW87_001483"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ5174628.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFSSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="WIW87_001484"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="WIW87_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ5174629.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="WIW87_001485"
     CDS             6251..7120
                     /locus_tag="WIW87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ5174630.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1882 a         2119 c         1722 g         1481 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagtcgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcaacctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc ggccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggaccc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgaccatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacaaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagat ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaaa tcacaccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggcg tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggtgt gaaagaaatc atcatggtcg tgccgacacc
     3301 aaaaggcgaa cgcaacgaca tcgtacttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaacccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtgttgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggttcg gacgaactga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aaacaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgcttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgatgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg caggcaggat tcccaacctg atagtcttac gcaccctcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ttatgcggca ggctgccccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ttgagcctga ccactgccaa
     4921 actcgccctg cggcactacg gcattatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttt caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agccccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cccgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacgc acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctcgcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattcccg cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaactggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgcttaaaat cagtcccgat
     7201 tatg
//
LOCUS       DAWISC010000108         7161 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWISC010000108 DAWISC010000000
VERSION     DAWISC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.108"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            267..488
                     /locus_tag="WIW87_001486"
     CDS             267..488
                     /locus_tag="WIW87_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174631.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKEDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
     gene            675..947
                     /locus_tag="WIW87_001487"
     CDS             675..947
                     /locus_tag="WIW87_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174632.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            1043..1909
                     /locus_tag="WIW87_001488"
                     /pseudo
     CDS             1043..1909
                     /locus_tag="WIW87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            1928..2191
                     /locus_tag="WIW87_001489"
     CDS             1928..2191
                     /locus_tag="WIW87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174633.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            2298..4220
                     /locus_tag="WIW87_001490"
     CDS             2298..4220
                     /locus_tag="WIW87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5174634.1"
                     /translation="MQLLAAEGIHQHQLNVQKSTRFIGIKVGKSNYSKNELNETKLPV
                     RVIAQTAKTRSGWDTVLEGTEFKTTLSGADIQAGVGEKARADAKIILKGIVNRIQTEE
                     KLESNSTVWQKQAGSGSTVETLKLPSFEGPALPKLTAPGGYIADIPKGNLKTEIEKLA
                     KQPEYAYLKQLQTVKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTSGAGTG
                     AVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLMVAVATA
                     GVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALV
                     NTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEILGETLL
                     DGRDPGSLNVKDRAKIIAKAKLAAGAVAALSKGDVSTAANAAAVAVENNSLNDIQDRL
                     LSGNYALCMSAGGAESFCESYRPLGLPHFVSVSGEMKLPNKFGNRMVNGKLIINTRNG
                     NVYFSVGKIWSTVKSTKSNISGVSVGWVLNVSPNDYLKEASMNDFRNSNQNKAYAEMI
                     SQTLVGESVGGSLCLTRACFSVSSTISKSKSPFKDSKIIGEIGLGSGVAAGVEKTIYI
                     GNIKDIDKFISANIKK"
     gene            4229..4651
                     /locus_tag="WIW87_001491"
     CDS             4229..4651
                     /locus_tag="WIW87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174635.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            4688..5089
                     /locus_tag="WIW87_001492"
     CDS             4688..5089
                     /locus_tag="WIW87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ5174636.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            5212..5832
                     /locus_tag="WIW87_001493"
     CDS             5212..5832
                     /locus_tag="WIW87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174637.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSVAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIRQVLDQLPD
                     GSPAKAAVFKANKNGTLKTAIAGVDRQTGKAVILPVKVPSKANIRR"
     gene            5835..6770
                     /locus_tag="WIW87_001494"
     CDS             5835..6770
                     /locus_tag="WIW87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ5174638.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         2268 a         1341 c         1662 g         1890 t
ORIGIN      
        1 tgaagaaaga tattttttat tgtgagcagt ggtcttatgg ttataagaaa cttcataagc
       61 ctttttctga gaaacaagct gaggaaaaac atcttaaagg ggagttatat actgccgtaa
      121 taggttcggc gacacaacct gaatatgtaa ttaccttgcg agaggaagta ggtttttttt
      181 cggtacattt tttcgataaa tttggaaggg attatttaac ccatcaattt caaaaatatt
      241 ccaattcgaa ttattatttt ctttctatgg ctgtatggag agattatata actttggaat
      301 ctcatgactt agcagaagga tatacttatt tcttcaatga aaatacggat gattgctatg
      361 ttttgaaaga ggattttatt aataatgagc gatatgaaaa aacagaatta tattcccaaa
      421 aagataaggt aattctattt ccaaagtttg gcgaatatga tttggtgtta aatccggaca
      481 ttatttaatt gagttttaag gccgtctgaa aaaatttcag acggctttta ttattgggtt
      541 tggaatctga ggataaagct gataaaaacc aggaaattat caggttgcta tatacgtatt
      601 gttgtacaga ctaaaggcag caatcaaatc actactgctt acccacaaaa ataaatcgat
      661 tatatggagt aatcatgaat aagagaatga aaatgtgtcc tgcttgtcaa caaggctatc
      721 tctaccattc gaaacctaaa tatcttcatg atgaaattat tctgtgtgat gaatgcgatg
      781 cagtatggct caaaggtatg aatatatttt atggagaata tgaaaaagat ttttattctt
      841 atgttccttt catggaatcc caaggtataa cgagtgaatg tatttgggaa ggagatttgt
      901 ttgatcatcc atattatgaa gatgaaaact caaatgatat ggattgatgg aaattttaag
      961 cctgcgtagg tacgattagc catcaaacgg cgtaatcata cgcaagatta tcaacagaga
     1021 gggctggcag cgatatacca cccacaagat tgcccatgcc atagcgggct gtgcggcagc
     1081 ggcggcgaat aagggcaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     1141 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     1201 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     1261 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     1321 agatattatt tgggatattg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     1381 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     1441 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     1501 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     1561 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     1621 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     1681 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     1741 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     1801 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     1861 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     1921 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     1981 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     2041 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     2101 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     2161 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     2221 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     2281 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcaccaa caccaattga
     2341 atgttcagaa aagtacccgt ttcatcggca tcaaagtggg taaaagcaat tacagcaaaa
     2401 acgagctgaa cgaaaccaaa ctgcccgtac gcgttatcgc ccaaacagcc aaaacccgtt
     2461 ccggctggga taccgtactc gaaggcaccg aattcaaaac caccctttcc ggagccgaca
     2521 tacaggcagg ggtgggtgaa aaagcccgag ccgatgcgaa aattatccta aaaggcatcg
     2581 ttaaccgcat ccaaaccgaa gaaaagctgg aatccaactc gaccgtatgg caaaagcagg
     2641 ccggaagcgg cagcacggtt gaaacgctga agctaccgag ctttgaaggg ccggcactgc
     2701 ctaagctgac cgctcccggc ggctatatcg ccgacatccc caaaggcaac ctcaaaaccg
     2761 aaatcgaaaa gctggccaaa cagcccgaat atgcctatct gaaacagctt cagacggtca
     2821 aggacgtgaa ctggaaccaa gtacagctcg cttacgacaa atgggactat aaacaggaag
     2881 gcctaaccgg agccggagcc gcaattatcg cactggccgt taccgtggtc acctcaggcg
     2941 caggaaccgg agccgtattg ggattaaacg gtgcggccgc cgccgcaacc gatgcagcat
     3001 ttgcctcttt ggccagccag gcttccgtat cgttcatcaa caacaaaggc aatatcggta
     3061 acaccctgaa agagctgggc agaagcagca cggtgaaaaa tctgatggtt gccgtcgcta
     3121 ccgcaggcgt agccgacaaa atcggtgctt cggcactgaa caatgtcagc gataagcagt
     3181 ggatcaacaa cctgaccgtc aacctggcca atgcgggcag tgccgcactg attaataccg
     3241 ctgtcaacgg cggcagcctg aaagacaatc tggaagcgaa tatccttgcg gctttggtga
     3301 atactgcgca tggagaggca gcaagtaaaa tcaaacagtt ggatcagcac tacattgccc
     3361 ataagattgc ccatgccata gcgggctgtg cggcagcggc ggcgaataag ggcaagtgtc
     3421 aagatggtgc gatcggtgcg gcggtcggtg aaatccttgg cgaaacccta ctggacggca
     3481 gagaccctgg cagcctgaat gtgaaggaca gggcaaaaat cattgctaag gcgaagctgg
     3541 cagcaggggc ggttgcggcg ttgagtaagg gggatgtgag tacggcggcg aatgcggctg
     3601 ctgtggcggt agagaataat tctttaaatg atatacagga tcgtttgttg agtggaaatt
     3661 atgctttatg tatgagtgca ggaggagcag aaagcttttg tgagtcttat cgaccactgg
     3721 gcttgccaca ctttgtaagt gtttcaggag aaatgaaatt acctaataaa ttcgggaatc
     3781 gtatggttaa tggaaaatta attattaaca ctagaaatgg caatgtatat ttctctgtag
     3841 gtaaaatatg gagtactgta aaatcaacaa aatcaaatat aagtggggta tctgtcggtt
     3901 gggttttaaa tgtttcccct aatgattatt taaaagaagc atctatgaat gatttcagaa
     3961 atagtaatca aaataaagcc tatgcagaaa tgatttccca gactttggta ggtgagagtg
     4021 ttggtggtag tctttgtctg acaagagcct gcttttcggt aagttcaaca atatctaaat
     4081 ctaaatctcc ttttaaagat tcaaaaatta ttggggaaat cggtttggga agtggtgttg
     4141 ctgcaggagt agaaaaaaca atatacatag gtaacataaa agatattgat aaatttatta
     4201 gtgcaaacat aaaaaaatag gagttagtat gaaatatatg attagttttc taaaaaaaac
     4261 atttgaatta atgagttggg tgttagtcat actaataatt gggacatttt atgactatta
     4321 tcaaataagg caatatgctg aattagaaaa gaaatctata tcaaatatct tgctatatgc
     4381 ccaaaaagaa aaatttcgct tagagagtaa agataaatac atgcgaggag gatatacaaa
     4441 atataaattt attttttcag aatatagtaa tactactttt ttaaatttca taaatgacct
     4501 gaaaaaagat aattatttac cacttgacgg ctatggacat ggttttctat gtagaaaagg
     4561 agagtctata tcaatcaata tataccctga aattaataaa tttattttag tatggggata
     4621 ccctgaaaat ctttgcgctg attctaatta agtaagcaag aataggttat agggaaataa
     4681 aattcaaatg agaaaattga ataatcacga tgttcataaa cggtatcaag atcgtttaga
     4741 agaggatgta gagttcacta tcaactatga gcttcctttg agttgtttgt ggtcaaccat
     4801 caaagacttt tccagcgatt ttgaggaaaa aactgaagcg ttctttattc ttttcaaaga
     4861 gctgctgcgc agaggtcatc tgaaactgca acgcgacggg caaattatcg ggcatacgcc
     4921 cgaagaatgg gaacaaatat ttagggaagt atggcctgaa tatgaaatcg aacccaatcc
     4981 acttcccggc tatgccccat ttgatattgg aatgtggctt acggtcgagg ctcctgccta
     5041 cgccgtatgg atagatcccg aagacggtag cgaatactgg gcgggataaa ataccaatgt
     5101 ttggaataaa tcccgtctga aaaacagctt tttcagacag gatttattcc aattatcggt
     5161 gatatacaga gttttgtaca agcacagacc gctgccgatc acctgtttgc tttgctgggt
     5221 gtggttccgg gtatcggtga atcgatacag gcctataaag tagcgaaagc ggcaaaaaat
     5281 ttacaaggca tgaaaaaagc cttggacaag gcagcaaccg ttgccactgc acagggctat
     5341 gtcagcaaaa ccaaaatcaa aatcggtcaa attgaattaa gggttactgc agcaactgac
     5401 aaacaattgc tgaaagctat tggcgaagga agggacacga caggtaaaat gaccgagcag
     5461 ttatttgact ctgtagctaa acaaaatggc ttcagagtgc tttcgggcgg caaatacggc
     5521 ggaaataacg gttttgatca tgtatggcag gctgccgatg gtagtgttgt tttgattgta
     5581 gaaagtaagc agattaggaa cggtacggta cagctgaatc cgaatggtgc gggtggatat
     5641 acgcaaatga gtgaggattg gattagacaa gttttagatc aattacccga tggtagtccc
     5701 gctaaagctg ctgtcttcaa agcaaataag aacggcacat taaaaacagc aatagcaggc
     5761 gttgatcgtc aaacaggtaa ggccgttatt cttcctgtca aagttccttc taaagccaat
     5821 ataaggagat aacaatgggg cacaatatga tgaccaccca aaaatggtat gagcatatta
     5881 ctaatgtaat cataggcaat actgctaatt tcaatagcgg ttgccttgac tctatagatt
     5941 atgtagatga aagaaaaggc gttccgcttg cagctatgca acatattttc atggacgtta
     6001 gagctgcagc ttcccatgcc tatctatttg aacatgatct taagaaattc aagcaatatg
     6061 cttatgttgc aggaaagctg ggggttttgc tgagtgtaaa ttctacagac cctgaaccct
     6121 tcttctttcc ctgtgacatg ctcaacattc aaaatccgat gtttctgatg ctgatgagcg
     6181 acagcccaca gctgcgcgag tttctggtgc gcaatatcga caacatcgcc aacgatacag
     6241 aagcctttat aaaccgctac gacctcaacc gtcatatgat ttacaatact ctgctgatgg
     6301 tagagggtaa gcagcttgat cggttgaaac aacgtagcga gaaagtcttg gcgcatccca
     6361 cccctagcaa atggctgcaa aagcggttgt acgattaccg cttcttcctc gctttcgccg
     6421 aacaggatgc cgaggcgatg aaggccgcct tagagccgct tttcgataaa aaaaccgcgc
     6481 gtatggctgc caaagaaaca ttgtcctatt tcgatttcta cctgcagccg caaatcgtta
     6541 cctacgccaa aatcgcatcc atgcacggtt tcgatttggg catagatcaa gaaatcgcac
     6601 cgagggattt gattgtttac gatccgctgc cggcagacga atatcaagac atcttcgatt
     6661 ttatgaaaca gtatgacttg tcttacccgt atgaatatct gcaggattgg atagattact
     6721 atacgttcaa aaccgataag ctggtatttg gtaacgcgaa gcgagagtga gccgtaaaac
     6781 tctgagctcc tgttttatag attacaactt taggccgtct taaagctgaa agattttcga
     6841 aagctataaa ttgaagccct tccatagtac atagatctgt gttgtggcgg ggctttacca
     6901 cgctgattgc cggagaagaa ctcaacctgc tggcaaaaca aggcatgaga tctttgcaat
     6961 aacatgagtt gagacctttg caaaaaagcc cttccccgac atccgaaacc caaacacagg
     7021 atttcggctg ttttcgtatc aaatacctcc taattttacc caaatatccc cttaatcctc
     7081 cccggatacc ccataataga gcatccgctc cgccttttag gcggtagcga gcgtacttag
     7141 cctgttggcg gctttcaaca g
//
LOCUS       DAWISC010000109         7113 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWISC010000109 DAWISC010000000
VERSION     DAWISC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.109"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(150..590)
                     /locus_tag="WIW87_001495"
     CDS             complement(150..590)
                     /locus_tag="WIW87_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174639.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(831..1010)
                     /locus_tag="WIW87_001496"
     CDS             complement(831..1010)
                     /locus_tag="WIW87_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ5174640.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            complement(1016..1603)
                     /locus_tag="WIW87_001497"
     CDS             complement(1016..1603)
                     /locus_tag="WIW87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ5174641.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTQEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(1625..2371)
                     /locus_tag="WIW87_001498"
     CDS             complement(1625..2371)
                     /locus_tag="WIW87_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ5174642.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIYS
                     FIDRCYTETLQYFDREEWGRLKEGFVRDACAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGFSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEDNSPGQNHLSA"
     gene            complement(2361..3203)
                     /locus_tag="WIW87_001499"
     CDS             complement(2361..3203)
                     /locus_tag="WIW87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ5174643.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFSELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            complement(3319..3627)
                     /locus_tag="WIW87_001500"
     CDS             complement(3319..3627)
                     /locus_tag="WIW87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174644.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(3700..4149)
                     /locus_tag="WIW87_001501"
     CDS             complement(3700..4149)
                     /locus_tag="WIW87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ5174645.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPRCRLKQD"
     gene            4494..5387
                     /locus_tag="WIW87_001502"
     CDS             4494..5387
                     /locus_tag="WIW87_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ5174646.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAVRKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5490..6593
                     /gene="prfB"
                     /locus_tag="WIW87_001503"
     CDS             5490..6593
                     /gene="prfB"
                     /locus_tag="WIW87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ5174647.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFRIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            6672..7031
                     /gene="rnpB"
                     /locus_tag="WIW87_001504"
     ncRNA           6672..7031
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIW87_001504"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1797 a         1624 c         1886 g         1806 t
ORIGIN      
        1 gatggaagcc ggttttaaaa aataatgtgg ataactttgt ggataatttt cagggaatgg
       61 caaaatgccg tctgaaatgc tgatatttca gtatttcaga cggcattttt atgataaggt
      121 ttgtgggtaa gtttgtgtat aagttgcaat cagagttttt ttccgcatac ggtttcatat
      181 tgtttttcgg ttaatgtccc ggacaggatg ctcatcgggc ggagggaaga ggctgtgtag
      241 ttttgtgtgg aaatttccgt gttttttgta tcaaacaact gtagcgaact taagcggtat
      301 gttttgttgt tgcagtggat ttcccactcg gcaatggcag tcttgtatgc gggggtgttg
      361 gcaaaacgtt cttgttttag attggtaaca acttttttat cttggaaaat catcagattt
      421 ccgttttttc tcacgctgtc tttattgata tatgtatgaa tattgccgtt tgagattgtg
      481 ccgatatttt cccatcgggc agacttgtcc ggtgtgctgc aggcggttat caaaataggg
      541 aataaaacga aagataggaa gattgggcgc attagggttt ccttaatcat tgaaatctgc
      601 tcattcagaa agaacgggtt gggcgggagg ggtagcgccg tctgtgaaat aaggcaaata
      661 aagcaaatat ggcaaattga tgcagccttc gtattttgcg tatttataca ttccgtatgg
      721 ggaaatatgc tgcttttgaa gtgtgtatta tggatgaact gcagataaca gtaaatcggt
      781 ttgatttgtt tccgtcagct tatgccgtct gaagcctttt cagacggcat ttattttgaa
      841 gttctggttg tttttgccag tgatcttttg atggtttgaa gttgtctttt atattcacgg
      901 caatacggac agaacagcag gtgtgtgtat atggaaatct tctcgcccgg ggtggtttcc
      961 cggtcctgat gtttggaaag aagcagggcg atatcgcggc attttttcat aacgtttact
     1021 tcgggttttc ttggttgaac catttgattt gcaggcattg gcgcaatgat tctcgggcgc
     1081 ggtgcataat ggtgtggtag ttggacgtgc tgataccgca catttgttgt atttcgtcgg
     1141 atgaaaaacc gagtatttcc ttcagggtaa atacccgtgc ggtgttttca ggcaggttgt
     1201 ataggcagct ttgcagaatt ttttggaatt cgttgttgtt taatgatttt tccggagtgt
     1261 tccaatgttg cggctgccct tcctgcgtcc aatgcccgtt ttgggaaaaa tggctttcaa
     1321 atgcttcatc cagtagctcg tcatccagtg tggtaaagac tttcctctgc cttccgattt
     1381 gacgtaatgc gtcaataatt ttgtttttca atatggcaaa aagccagctg ttgacaagtg
     1441 ccctgccttg aaaactgtcg cctgcgctgt atgcggacag caatgtttcc tgcactaaat
     1501 cttcagccaa atcagggtgg tcgggcaact gaagccgcgc aaaatgcaga agcagtttac
     1561 gcgactctat taattcggca tcggtcaggt cgggtagcgg catagtatcg ggaacaaggt
     1621 aattttatgc ggatagatgg ttttggcccg gggaattgtc ttccatattt tcggatatgg
     1681 cggacaagga ggggatgata atcctttgtt cagtccagcc tgaccatttc ccaagcaaaa
     1741 tatttttcca attatcgtcc tcaggcataa attcgacaag ggtttgggcg agggtgtcaa
     1801 acgaaagcgc ggagaacccc attatttcta gcagcatcat atcgaagccg tccaatgttt
     1861 ggtacatcac atcatcttcg gcgtttcgcc atattaacaa ggctgtttcc gcttcatgca
     1921 aatcatcggt aacatcatat cgatattgcc ggataaaggc cgcaggggaa ggtgtgtatt
     1981 tgctgtcatt tgaataatga atgtcgggaa tatccggaat ttgagcaact tctgccagca
     2041 attgggtata ttcaaaatcc atcagtgcca aaatgccgtc taaaagcggc aggctttggc
     2101 aatattggag gaactcgccg gggatttctt gaaaataggg cgtttgggcg cacgcgtcgc
     2161 ggacgaaacc ttctttcaga cggccccatt cttcgcggtc aaagtattgc agcgtttcgg
     2221 tataacaacg gtcgataaag ctgtagatat tgttgcgtat caggcggata tagacgttca
     2281 gtcggtcttg cggcagaccg tctgcggctt cgcccccgcg tatggcttgg gcgaaacggt
     2341 gctggtattg ggcggaggtt tcaggctgca cggcggcatt cctttccggc acgcgtttga
     2401 taatcggcga ttttggcgac ttcggcttcg agttcggaaa aaggcgggaa attaaaatcg
     2461 cgttccaaca gggtgggcgg aatcgtcggc agcttggcat aggcaagttc gagcaagtcc
     2521 caaacagtcg gcaaaactgc cgcgccatgt gtatcaatca acaattccgg cgtttccacg
     2581 tcatgtccgg caatatggat atagcacacg cgctctgcat ccacattttc caaaaaagcc
     2641 tccggcgaca gcagaccgtg attgacggcg ttgacgtaga tattgttcac atccagatga
     2701 atgccgcaat cggcctcacg tgcgacggcg ttgaggaact cgacctcgtt catctcggca
     2761 agcggggaat gcagatagta ggacgtgttt tccacggcga tgcggcagcc caaacggtct
     2821 tgcacttcgc ggatacgccg cgccgtatga tgcaccattt cctcggtaaa gggcagcggc
     2881 aacaaatcgt aaagatgacc gccgtcgtgg cagtagctca aatggtcgga gaaaaacgtg
     2941 caatcgtaac ggcgcatcat ttctttgatg ccgtctatca aatcagtatc cagcggtgct
     3001 tgcccgccca gcgacataga caatccgtgc aacgccagcg gcagccgttc cgccacacgg
     3061 tcaaactgtt tgcgcgccca gccgcccatt ttcagccagt tttccggtgc ggcttcgata
     3121 aagcatatcg ggctgttttc ggaaagcgag agaaagtctt ccgccaagtc gcggcggtag
     3181 cccaagcctg cgtgttgaat catattttta cccggttcgg atggcacaaa agaatgatgt
     3241 gcttgttaaa aaatctttgt ttgccgttaa aaaagccgca ttttactgaa aaacgcggct
     3301 tggtttgaag gggtgggatt atttagaacc gcatttgcct tcgccgcatt tgccttcggc
     3361 agatttggcc ttggctttag atgctttggt gtgtttgtgg gtttttttta cggtcgcacc
     3421 gcatttgccc tcgccgcatt tgccttcgcc tgctttagaa ccagccgcgc cgcacgaacc
     3481 ttcggcagat ttggacgcgc cgcaagagcc atgggcggat ttatgaacgc ctgttgcgtt
     3541 gcttgccggt ttgttggcag caactgcacc ggcggccaaa gacagggata aagcaccggc
     3601 gagagcggca gcaatgtttt tgttcatgtc atttcctttt caaaatatca agttaacgag
     3661 ataaggttgg aatgtttaaa gtcagattcc acgactgaat caatcttgtt tcagacggca
     3721 tcgggggcaa aaccgttttt tcagcagcgt atccagcgac catccgcccg caccctggaa
     3781 aagcagcggg attaatacca cgatataaat taaagccatt ttataaccgt tgtcgcagac
     3841 attgtaaccc gaaccggcgt gaaccgcagc ccaagcgacg gcggtaacga ccatcagccc
     3901 caatgccgac agacgcgttg ccaaacccaa aagcaacaat acgggcagca aaagctccgc
     3961 atacatggcg agattccagt ttaacgcgtc cggcagcaag ttgaacggga atggaaactg
     4021 atcgttgatt tcggaaaacc aattctcccc gttccatttt tgcaaacccg attccaaaaa
     4081 ttcatatgcg gcaaacagac gcaaaaccag catagcggat gccgtctgcc aagttgttcc
     4141 gattttcata agtgttcctg ttttggttct gttgataccc cattagacgg attttgtcgg
     4201 aaaaactgac acccggcagc caatgattta acaagttaat gaatataaaa agaaagtatt
     4261 ttgatggaaa gaaagggaaa aaggcgggat atttggataa cggcggtgtt gtgagataat
     4321 tcccgccttg gatagtatgc cttcaaatat aatttccgta aaggaaaaaa aaaagaaata
     4381 ttaattttga tgatgactta acaaagcccc catttgcttg gctgtcaaag gggggtgtta
     4441 agaaaagcaa tccctctttt tgacggggtg cgatatataa aggaatggaa aatatggtaa
     4501 cgttttcaaa aatcagaccg cttttggcaa tcgccgccgc cgcgttgctt gccgcctgcg
     4561 gcacggcggg aaataatgct gtccgcaagc cggtgcaaac cgccaaaccc gccgcagtgg
     4621 tcggtttggc actcggtggc ggcgcatcta aaggatttgc ccatgtaggt attattaagg
     4681 ttttgaaaga aaacggtatt cctgtgaagg tggttaccgg cacatcggca ggttcgattg
     4741 tcggcagcct ttttgcatcg ggtatgtcgc ccgaccgcct cgaattggaa gccgaaattt
     4801 taggcaaaac cgatttggtc gatttaacct tgtccaccag tggttttatc aaaggcgaaa
     4861 agctgcaaaa ttacatcaac cgaaaagtcg gcggcaggca gattcagcag tttcccatca
     4921 aatttgccgc cgttgctact gattttgaaa ccggcaaggc cgtcgctttc aatcagggga
     4981 atgccgggca ggctgtgcgc gcttccgccg ccattcccaa tgtgttccaa cccgttatca
     5041 tcggcaggca tacatatgtt gacggcggtc tgtcgcagcc cgtgcccgtc agtgccgccc
     5101 ggcggcaggg ggcgaatttc gtgattgccg tcgatatttc cgcccgtccg ggcaaaaaca
     5161 tcagccaagg tttcttctct tatctcgatc agacgctgaa cgtaatgagc gtttctgcgt
     5221 tgcaaaatga gttggggcag gcggatgtgg ttatcaaacc gcaggttttg gatttgggtg
     5281 cagtcggcgg attcgatcag aaaaaacgcg ccatccggtt gggtgaggag gcagcacgtg
     5341 ccgcattgcc tgaaatcaaa cgcaaactgg cggcataccg ttattgacgc tatgccgtct
     5401 gaacgtgttt ccagaaggct ttatccatag aaaaaaatac ccgaatcccg tacaatacgc
     5461 ccctaaaatt ttgaaagaac aaaagaatca tggaagccga agtaatcaac cagctcaaca
     5521 ataccctgaa cgatttggaa aagcgcagcg aagacatccg agtttatatg gattatcagg
     5581 gtaagaaaga ccgattggaa gaagttatcg gtctttccga agaccccgaa ctttggaacg
     5641 acccgaaacg cgcccaagaa atcggcaaag agcgcaaaat cctcgaaggc atcgtgttga
     5701 cgctcgacaa catcgcttcg ggcatcgaag acaaccgcat gctgattgaa atgaccgtcg
     5761 aagaaaacga cgaagaaggt tttgccgccg tgcaggaaga cgtagcgggg ctggaaaaac
     5821 agatggcgga tttggagttc aaacggatgt tcaaccagcc cgccgacccg aacaactgct
     5881 ttatcgacat caccgcaggc gcgggcggta cggaagcgga agactgggcg ggtatgctgt
     5941 tccgtatgta cagccgctac gccgagcgca aaggcttcag aatcgaaatc cttgaagaag
     6001 acgacggcga aatcgcaggc atcaaccgcg ccaccatccg tgtggaaggc gaatacgctt
     6061 atggtttgct gcgtaccgaa accggcgttc accgcttggt gcgttactcg ccgtttgact
     6121 cgaacaacaa acgccatacc tcgtttgcct ccgtgttcgt ttaccctgaa atcgacgatt
     6181 ccatcgaaat cgaaatcaac cccgccgatt tgcgcatcga cacctatcgc gcatcgggtg
     6241 cgggcggtca gcacatcaat aaaaccgact ccgccgtgcg cattacccac gagccgacgg
     6301 ggattgtggt gcaatgtcaa aacgaccgtt cgcaacacgc caacaaagcc gctgcgatgg
     6361 aaatgttgaa gtccaaactg tatgaattgg aaatgcgcaa acgcaatgaa gagaaacagg
     6421 cgttggaaga aggtaaatcc gatgtaggtt ggggcagcca aatccgttcg tatgttttgg
     6481 attcctcacg tatcaaagac ttgcgtacag gctacgaagt cggcaacacc aaagccgtat
     6541 tggatggcga cttggacggc ttcatcgaag ccagcctgaa acagggcgtg tagtttaggc
     6601 tgtatgccgt ctgaaacctg taccgtttca gacggcattt ttgtgtttcg tgttataatc
     6661 cgcgccgcac acgggacggg cagacagtcg ccgcgtatcg cgtaaggcat acggggagga
     6721 aagtccgggc tccgcagggt agaatgccgg ttaacggccg ggcgcggtaa cgcgacggaa
     6781 agtggaacag aaagcaaaac cgccgatggc tgctttggca gcacaggtaa gggtgaaaag
     6841 gtgcggtaag ggcgcaccgc gcatttggta acaatatgcg gcaggccaaa ccccattcgg
     6901 agcaagacca aacagaacgc aatgacgctg cccgccgagc gttcgggtag gttgcttgag
     6961 cataccggta acggtatgcc tagaggaatg actgtccgag acagaacccg gcttaccgcc
     7021 tgtccggtgt gcatccgaca cgcaaatgtc gtctgaaacc tgaaatatgg ttcagacggc
     7081 atttttattt aggttgtgtt tttatagtgg att
//
LOCUS       DAWISC010000110         7079 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWISC010000110 DAWISC010000000
VERSION     DAWISC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.110"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WIW87_001505"
     CDS             complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WIW87_001505"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ5174648.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWGEIRAEENEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIML
                     SEHVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATV"
     gene            complement(3156..3368)
                     /locus_tag="WIW87_001506"
     CDS             complement(3156..3368)
                     /locus_tag="WIW87_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174649.1"
                     /translation="MGFWNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIV
                     KSDGFFKNSTREKSAAYAILKERGEV"
     gene            complement(3501..3974)
                     /locus_tag="WIW87_001507"
     CDS             complement(3501..3974)
                     /locus_tag="WIW87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ5174650.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4137..5144)
                     /locus_tag="WIW87_001508"
     CDS             complement(4137..5144)
                     /locus_tag="WIW87_001508"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ5174651.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTDNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGEDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            5376..5612
                     /gene="acpP"
                     /locus_tag="WIW87_001509"
     CDS             5376..5612
                     /gene="acpP"
                     /locus_tag="WIW87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5174652.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5768..7015
                     /gene="fabF"
                     /locus_tag="WIW87_001510"
     CDS             5768..7015
                     /gene="fabF"
                     /locus_tag="WIW87_001510"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ5174653.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDTS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1599 a         1888 c         1920 g         1672 t
ORIGIN      
        1 aaaagtagaa tgcgctattt taatggaaac ggcggggttt taaacggttc ttatttgttt
       61 gttttgattt ttgcttggat aaaaaatccc atcattccca caaaaacaga agcctgaaat
      121 cccgtcattc ccgcgaaaga ggaatccggt ttttgggttt cagccattcc cgataaatcg
      181 ctttagctct gccgcaacct taaaacggca cggtacgaaa ataccgtctg aaacccagat
      241 tgtcaggctt cagacggcat tttttgttca gacggttgcc gcttcttcgc cgaacacttc
      301 ccgccacaat ttcctgacat tgccgtaatg tgccaacagt tcgccggtta cttcggtttt
      361 tgccgcgtcg cgcagtttgg tgttgtgctg ctgccggcgg tagaagcgat aggcggtgcg
      421 gctttgtccg gcgagggttt tgtcaatcaa accgcagtcg gcggagatgt ttaagagggc
      481 gatgttgccg tagttgtcca agagttgcgg atactggcgg gcatgggcaa gtatcagata
      541 ttggacgata aattcgacat cgaccacgcc accgcgcgcg tatttgacgt tgctgtcggc
      601 aggcgggtgg gtggggaaca ttttttcgcg catttcgatg atttcgcctg ccaaggcggt
      661 ttggtcgcgt tcggcggtga ggatttcggt gcggatgcgg tcgaaggccg tctgaatttc
      721 ggacgtgccg cagatgaagc gggcgcgggt aagggattgg tgttcccacg tccaggcgtt
      781 ttcgcgctgg tatttttcaa aggcggcgat gctgtgggcg aggaaaccgg cgtcgccatt
      841 agggcgcagg cgcaggtcgg tttcgtagag gctgcctgcg ccagtggcgg cggaaagcca
      901 gttggtcagg cggcgggcga ggcggctgta cacgtcgcct gcgtcggggt gggggtcgtc
      961 gtagagatag acgaggtcga ggtcggaggc gtagccgagt tctttaccgc cgagtttgcc
     1021 gtagccgacg acggcgaatt gcggtgtgtc gcggtgtttt ttgggcatgt ccgcccatgc
     1081 gcacagcagg gcggcggcga ggatggtgtc ggcgagggcg gagagttggt cggagaggga
     1141 ttctaccgtc cacagtccgg cgaggtcttg gacggcgaga cggaagactt gggcgtgctg
     1201 gaagcggcgc agggtgtcca tttgcgcttc agtatcgccg ccgcaggctt tgaggtcgtc
     1261 tgaaagggcg gcggcgagcg cctgccaatc aaacgcggta tccaaaagct gcgcgctgat
     1321 gagttcgtcc aacaaaatcg gatatttgtt cagatacgcc gccacccaag aactttggcc
     1381 cataatctgc gccagttgcg ccaaggtttg cggatgttcg ttgaggaagg cgagataggc
     1441 ggatcggcgg ctgatgtttt cgagaaaatc caacagccgc atcaatgtat cggtcgggtt
     1501 gctttgcgct gccgccgcct gtacgaacag cggcacaacc gcatcgaaac gcggctgggc
     1561 gtgtgcggaa agatggcggt atttatggcc gtggcggatt tggtcgagcc ttgcggcgac
     1621 ggtttcggca tcgaacccgt gcgccttcag acggcatcgc cgcccttctt cgtcgggttt
     1681 gtcctgccat gcccattgcc attcgctgtt gtcttgcgtt tgctcttcgg gttcgctcaa
     1741 aatttcgttg aacaactgat tgactttgtt ccgatgaaca ttgagaccgt ctgaaaaagc
     1801 ggaataactg tcgaaaccca tgctttcggc gagcagttgc cgctgttcgg gcgaggtcgg
     1861 cagggtttgg gtttgctggt catcccagta ttgcaggcgg tgttcaacat cgcgcaggaa
     1921 gcggtaggcg gcaagcaggg tttcgacgtg ttcagacagc atgatgccca gctcggcaag
     1981 cttcttcagc gtttcctgcg tgcctttcag ttgcagcgcg cgcatttgtc cgccgcgtat
     2041 catctggaaa atctgggcga taaattcgac ttcgcggatg ccgcccgcgc cgagtttgat
     2101 gttgtccgcc atgccttttt tgctgacttc gctgctgatt tggcggtgca gcttacgcat
     2161 cgcctcatac gcgccgtaat ccagatattt gcggaacaca aaggggcgca ccagtgcttt
     2221 gatgtcgttc ggatacggcg taaccacgcg acctttgcac cacgcgtagc gttcccattc
     2281 tcgcccctgt gtaatcaaat attgctccag cgcggtttcg ctcaatacca acgcgcccga
     2341 atcgccgtcc ggccgcagcc gcatatcgac gcggaacacc tgcccatcgg cggtaatgtc
     2401 gttcagcagc gcaatcagtt tctgcccgac tttggtgaaa aattcctgat tgccccgttc
     2461 gcgcctgccg tcggtgtcgc ctgattcggg atagacgaaa atcaaatcga tgtcggaaga
     2521 cacgttcaac tcatagccgc ccgccttgcc catcgccacc acgctcaaat gctgcggcga
     2581 tttggtataa cgcccgatcg gcgtgccgta catgtcccga taataggcgt aggcaaaatc
     2641 cagcgcggta ttgacggcaa aatcggcaaa cagcgtaatc gtgcgggtta cttcgttcaa
     2701 atcgctgata cggttgatat cgcgcacgat aatctgcgac accacataac ggcgcaactc
     2761 gcgcaactgc cgcgccaatt cttcctcgtt ttcttccgcg cggatttcgc cccagtcggc
     2821 aaaggcttgg aaatccgctt cggtcaaaac cttgtcgagc ataggcagga atatttcggg
     2881 cttgagtttg ccgttgtcga gctggcgggc gaggaagagg gaatggcggc gggcggtgtc
     2941 gaggcggttg tcggacattt cggattccgt ttggaaggat gacgggaatg agatagtgga
     3001 ttaactttaa atcaggacaa ggcgagggga tgccgtaccg gtttaaagtc aagccactat
     3061 atcatagcaa ccttcgatgt cgtctgaaac cttgccccga tattcggacg gatgcctacc
     3121 aataaaaaac agcggcaaat gccgccgttt cctgttcaca cctcgccacg ctcttttaaa
     3181 atagcataag ccgcactttt ctcccgtgtg gaatttttaa aaaaaccatc actcttgacg
     3241 atttcatgca gctcctcact tgatttctcc gcatattcca ttttcaacgc cttatgctca
     3301 ttgccggctt caatcattcc ctctcccact gcttttgctg cttttgccac accattccaa
     3361 aaacccataa tcatgtcctc caatcgtttc aaataaggtc gttgtttctg tcaagaaaca
     3421 acgacagttt ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag
     3481 acggcatcgg attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag
     3541 tgagaatata accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct
     3601 ttgggcggca tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa
     3661 attttttcaa cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt
     3721 aagaggacgt acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc
     3781 agaacaggct ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt
     3841 tcatcatgat ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag
     3901 tatttattcc cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt
     3961 aaatcaaggt tcatttttta ctccgattgc ctgctccgtg ttcacgcatc ggtttgcctt
     4021 atttcaaaca gggcggcgcg taagggattt tcaaggggcg tcaggcggaa aaaggaaatg
     4081 aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca ttttgggcgg atcgcgtcat
     4141 cgcgccaaag ccttcaaaca ttctttgacc aatgccggac ctttgtatat caatccgctg
     4201 tacacttgga cggcggtcgc gcccaagcgg attttatctg ccgagtcctc gccttccata
     4261 atgccgccta cgccgataat cggcagcttg ccgtctatgt ggtctgccaa cagcttcaac
     4321 acccgattac ttttttcatg aacgggcagc ccgctcaaac cgccctgctc gcctgcgagc
     4381 ggatggctgc cgagacttga tttgtcgatg gtggtattgg tagcgatgat gccgtccatt
     4441 tcgacggatt tgacaacgtg ggcgatgtct tcgatttgtg cttcatccaa atcgggggcg
     4501 attttgacgg cgagcgggac gtatttcccg tgtacagagg caagctgtgc ctgtttgttt
     4561 ttcaaagcct caagcaatgc gctcaactcg tcgccacctt gcagcgcgcg gaggttttta
     4621 gtgttgggcg aggaaatatt gacggtaatg taacttgcgt gtgcgtaggc tttttcaagg
     4681 cagattaaat aatcatcggc agcgttttcg atgggtgtaa ccgcgttttt accgatgttg
     4741 atgcccaata cgccactgaa tttacttttt tcgatgttgc gtatcatggt gtcgataccg
     4801 tggttgttga aacccatgcg gttgatgatg ccttggtgtt cgggaacgcg aaagaggcgc
     4861 ggctgcgggt tgccgggctg cgggttgggc gttaccgtgc cgatttcgat gaaaccaaag
     4921 ccgagcgcgc ccaatgcgtc gatgtattcg ccgtttttgt cgagtccggc ggcaagtccg
     4981 acagggttgg gcaaatccat acccatcaat tttacaggtt tggtacggtt gtcggttaca
     5041 ggaatcaaac ccaatttata aaccgtgtag agcgcgtcga gcgtgaagtg gtgggctttt
     5101 tcggcatcga gtgcaaacag gatgcgacgg gcaagtggat acatgacagg gctttctgct
     5161 tcagatggaa atgccgccat tttaaccgaa atcccgccaa acatacggca cacaggtcaa
     5221 acttgaacga caaaacccgc cgcacccaaa aatgtacaaa tcggcaaata ttgatataat
     5281 agtcattaga tagaaaactc tattaaaagt cggtacaata ccaccgattt atttccctta
     5341 aacaacctgc cgcaaggcat aaaggaacga ctgatatgtc aaacatcgaa caacaagtta
     5401 aaaaaattgt tgctgaacaa ctgggcgtaa acgaagccga cgtgaaaaac gaatcttcct
     5461 tccaagacga cttgggcgcg gattctttgg acaccgtgga gctggttatg gctttggaag
     5521 aagcattcgg ctgcgaaatc cccgacgaag atgccgaaaa aatcaccacc gtccaactgg
     5581 ctatcgacta catcaatgcc cacaacggct aaccggtcgt cgcccgacac aacagcctct
     5641 gctgcaccgc gcaatagagg cttttccctt atatggcaaa ctgtttgaac ccctgccgca
     5701 aattacagac ggcatctgca cgacagccgc atatgccccc attccgacaa ccaacagcga
     5761 ggttatcatg agtcagagaa gagtagtcat tacaggctta ggtcaggttt cccctgtcgg
     5821 caacactgtc gcagaggctt gggacaccct gctcaccggc aaaagcggca tcggcgcgat
     5881 tacccgcttt gacacatccg acatcaacag ccgtgtcgcc ggcgaggtgc gcggtttcga
     5941 catcggacaa tacatcagcg cgaaagaagc gcgccggatg gacgtattca tccactacgg
     6001 cattgccgcc gcattgcagg caatcgccga ttcgggtttg gacgatgtgg aaaacctcga
     6061 caaagaccgc atcggcgtga acatcggttc cggcatcggc ggactgcccg gcatcgaggt
     6121 caccggcaaa gccgtaatcg aaggcggcgc gcgcaaaatc aaccctttct ttatccccgg
     6181 ttctctgatt aatctgattt ccggacacgt taccatcctc aaaggctacc gcggcccgag
     6241 ctacgggatg gtttccgcct gcaccaccgg cgcgcacgcc atcggcgatt ccctccgtat
     6301 gattaaatac ggcgacgcgg acataatggt tgccggcggc gcggaaggcg caatcagcac
     6361 tttgggcgtg ggcggttttg ccgcgatgaa agccctctcc acccgcaacg acgaccccgc
     6421 caccgcttcc cgtccgtggg acaaaggccg cgacggcttc gttatcggcg aaggcgcggg
     6481 catattggtg ttggaagaat tggaacacgc caaaaaacgc ggcgcgaaaa tctacgccga
     6541 aatcgtcggc ttcggcatga gttccgatgc ttaccatatc accgcgccga acgaagaagg
     6601 ccccgccctt gccgttaccc gcgcgctgaa agatgccggc atcaatcccg aagacgtgga
     6661 ttacgtcaac gcgcacggca cgtccacccc cttgggcgat gccaacgaaa ccaaagccct
     6721 caaacgcgcg ttcggcgaac acgcctacaa aaccgtcgtc agctcgacca aatccatgac
     6781 cggccacctg ctcggcgcgg cgggcggcgt ggaggccgtg tacagcattt tggcgataca
     6841 cgacggcaaa atcccgccga ccatcaacat ttttgaacaa gacgttgaag ccggctgcga
     6901 tttggactac tgcgccaacg aagcgcgcga cgcggaaatc gacgttgcca tttccaactc
     6961 cttcggcttc ggcggcacca acggcacgct ggtcttcaaa cgcttcaaag gctgattccg
     7021 caaagccgcc gccgacatcg aaatgccgtc tgaaaccgtt tcagacggca tttttatag
//
LOCUS       DAWISC010000111         7062 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWISC010000111 DAWISC010000000
VERSION     DAWISC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.111"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(417..1109)
                     /locus_tag="WIW87_001511"
     CDS             complement(417..1109)
                     /locus_tag="WIW87_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5174654.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
     gene            complement(1106..1849)
                     /locus_tag="WIW87_001512"
     CDS             complement(1106..1849)
                     /locus_tag="WIW87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5174655.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(1878..2441)
                     /gene="gmhB"
                     /locus_tag="WIW87_001513"
     CDS             complement(1878..2441)
                     /gene="gmhB"
                     /locus_tag="WIW87_001513"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ5174656.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            complement(2689..3740)
                     /locus_tag="WIW87_001514"
                     /pseudo
     CDS             complement(2689..3740)
                     /locus_tag="WIW87_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(3890..5131)
                     /locus_tag="WIW87_001515"
     CDS             complement(3890..5131)
                     /locus_tag="WIW87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ5174657.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGDVSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWAAGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            5283..6011
                     /gene="ubiG"
                     /locus_tag="WIW87_001516"
     CDS             5283..6011
                     /gene="ubiG"
                     /locus_tag="WIW87_001516"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ5174658.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            6048..6965
                     /gene="thrB"
                     /locus_tag="WIW87_001517"
     CDS             6048..6965
                     /gene="thrB"
                     /locus_tag="WIW87_001517"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ5174659.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
BASE COUNT         1404 a         1671 c         2324 g         1663 t
ORIGIN      
        1 tcttaacccc gcgttctaga ttcccacttt cgtaggaatg acggcggtag atttggcaga
       61 tgcggcggat tcggcaggtc tcaacccatc ctacaatcca ccctgaccgc cttttacgaa
      121 tccgccgcca tcctgggaaa cgcaaaaaaa atgccgtccg aaaacctttc agacggcatt
      181 ttcgcgggca aatcagtaaa agacttcgcg tcagcttaag cgtttcatac cgcacgtcgg
      241 accgggcggg tttcgggttt cggaagagcg gtgtgaaatg aaacgcggca gacggcgtgt
      301 gtgccgccat cctgccccga accggcggca aagcgtcatc gcggtttgaa tcgggcgggc
      361 gcgtgcctgt tccggcacgg atgccgtctg aaagcggcgg gtcggtccgg tccgcgtcag
      421 cctaaggcga aaagttccct gccgtggatt ttgagccact gtttcgcttt gggcgtgtag
      481 ggggcgaagc ggcgggtcag ttcccaaaag gcggggctgt ggtcgggatg ggcgaggtgg
      541 cagagttcgt gtatgcagac atagtcggca acgtattccg gtgcgccgac cagccgccag
      601 ttgaagcgta tgcctgtggt tttgcggcac acgccccaga aggttttggc agaggtcagc
      661 gaggaggagg cggggaacag ttgtgtggtg cgggcgtggc gttcgaggcg gggaatcagg
      721 taactgtgcg cctgccgttc caaaaagtcc cgcagcagcg caagctgttt ttcgggtgcg
      781 ccttcgggaa cacggatttc agacggcatc agcaggattt gcgtgtcttg atgggcggtg
      841 agggcaagct gtctgccgtg gaagaggatg gattcgggca gccggttttc ggcagtttgc
      901 ggcggcggtg ttttcgccag tgtttgccgc aggacggctt cgttttcata cagccagcgg
      961 tttagagcgg agacggagaa gcagggtggg acgctgatgc ggacggtatg tgtgccggcg
     1021 gggcggataa tcaggttttt cttggcacgg cgcttgattt cgacggtcag ttccatgccg
     1081 tctgaaaggg tgtggacaaa ggcggtcatg cggtttcaga cggcattttg gcggcaaacg
     1141 ggcctgcgcc ggaaataagc ggttgttgcg tttcgatgag atgttcgcat ttttccatca
     1201 attcggcttc gctgccgctt gcgtgcggga tggtcggaca gatgacgacg gtgatttccc
     1261 ccggatattt cagaaaggag tttttcggcc aaaattcgcc gctgttgagg gcgacgggga
     1321 cgatgtccat ctcaaacatt ttcgccatgc gcgcgccgcc gagtttgtat ttgccgcgtt
     1381 ttccgggcgc aaggcgcgtg ccttcgggga aaatggtaat ccaatagcct tcgtttttgc
     1441 gcaccaaccc ctgttttatg agctgctcgt tggcttcgcg gcggttgttg cggtctatgc
     1501 ctatggtttt gaccagtttc aagccccagc cgaaaaaggg gattttgaac aactcgcgtt
     1561 tggcaacgta aacctgcggc ggaaaaatgt cctgaagggc gagcgtttcc cagccgcttt
     1621 ggtgtttggc gcagatgacg gcggggcggt cggggatgtt ttccgcgccg atgatgcggt
     1681 atttgagccc gacgatgtgt ttgagcgacc agttgagaat gccgacccag acccgcgcca
     1741 tcttgtgcgc cccgtcccgg aaaggcgagg cgagcagcat aaagggaaag aggaaaatca
     1801 gggtggaaca gagtatcagc cagtaaatca ggttgcggat gatgagcatg gttttgcctt
     1861 gtcggaatgc ggtatgttca gtcggcttgc ggtgcggtgt tttcctgcat gatgtattgt
     1921 gagaaatcga gcagggtatc gaaaacctgt gtgtgttcgg gcaattcgtg tccgtgttgg
     1981 gagagcgttt ttttgccttt tccggtcaga accagcgcgg gtttgccgcc gacggcatcg
     2041 attgcctgca aatcgcgcag gctgtcgccg accagccagg tttccgaggc ttgggcgttg
     2101 aagcgtccga tgatgtcttc aatcataccc ggtttgggct tgcggcagtt gcagttgtcg
     2161 gcatcggtgt gcgggcagaa ccagatgccg ttgatttcgc cgcctgcctg acggacgagg
     2221 cggtgcattt tggcgtgcat ttcggtaagg ttttgaacgg taaaatattt gcgcccgatg
     2281 ccggattggt tggtggcaac ggcgacggtg tagcctgcct gcgtcagaaa tgccaccgca
     2341 tccatgctgc cttcgacagg tatccactcg tcaacggatt tgacgaagtc gtcgcggtcc
     2401 tgattgatga cgccgtcgcg gtcgagaatg atgagtttca tcgcgtttcc tttggattgg
     2461 ggcaggtcgg gggtggcatt atactgaaat atcggtggaa atgcgcctgt gccgcgcgat
     2521 aacgcgcctg ttccggcaac cgcttgcaat gcgcgggtac ggcgtttcgg gggcggttgc
     2581 cgatgttttc ctgcctgctc ctgcaacggc ggattttctg ccggacgtat tttccgctgc
     2641 aaaagtctcg gcgcggaagt tgcggggcaa tgccgtttgg ggacgcggtc aggcggcaag
     2701 catttcaaga atcagcccga tgtgtttgtc taaagccggg ttgtagttca gtgcccgcat
     2761 cgggtcggac aggcggtgtc cgccctgttt tttaaggctg acggcttggg caacggcttg
     2821 cgccagggtt tccgggtttt ggcgtgagaa aaagaagccg gcttcttcgt tcatgacctc
     2881 tgtacatgcc atgttttcgg agaggacgac gcgtgtgccg cataggacgg attcgacgcc
     2941 gaccagcccg aagggttcgt acagggaagc cataatggta aagtcggcgg cgcggtagag
     3001 ttcgggcata tcggtgcaga agcccagtcc gacgacgttt ttcatagggc ggggaagcgg
     3061 ggagccgaca acggcgagct tgacgggcag gctggtatgt tcgaaaaagt cggcaagcag
     3121 ttccagacct ttgcgcgtgt ggccggtcga tgggaacagg aaaacggttt catggtcggc
     3181 aaagccgtat ttggcgcgca ggtcggcagt ttctccgggt tgtggaaaga agcgttccgt
     3241 atctgcgggg ggggggcgac ttggattctt tcagggggaa cgccgtacag tccgaccagt
     3301 tcgcgccgca tcatatggga atgcgccata atcagtttgg cggtggcgta gttgctgcgg
     3361 ttgcggcgta tggcgaggcg gtcgagcagg ttcggttttt gcgccatatg gtgcaggtag
     3421 cccaagtgtg tgccgccgca gatgaggagg tcggcgtaat cggcgtggtg gcaggcaatc
     3481 agtttggcgg cactgttttt tctggtttga gcgagccggc ttgaaaagag gaatgagcgt
     3541 agttttttca gcgtccggtg ttgatcgaca agatggggtt cgattagggc gtattcagga
     3601 atgctgtgat caaatttcgt cgcataaacg gccggtgtga tgttttgtct gttcagaccc
     3661 tttaccaaat ccaatgtgta gcgttccgtg ccgccgccgt gtttgaagtt gttggttgca
     3721 atgtctattt tgagcttcat cattgttcct ttatggttgc gtcccggttt gtcggggcgg
     3781 gatttgtgcg tgaggggcag ggtaatgcgc tgtgtgccgg aatacggttg ccgtttgttg
     3841 cggcaatgcc gtctgaagcc gccggcgggc ttcagacggc attttgcctt tatcctttaa
     3901 atacggggac gagttccatt tggctcaata cctgtgcggc gatgttgacg atgccgaaaa
     3961 ggaagaccca aaccatcagc cacaagccgc cgtaaacttt ataggttttg cctgcgccga
     4021 attttttgcg cgaacggtag agcagcatgg cggggatgat gcctgtccag acggttgccg
     4081 ccaggccgac gtagccgatg gcggtaacga agccggtggg gaagagcagg caggaaatca
     4141 ggggcggcag gaaggtcagc gcggcggttt tggtgcggcc ggagatgctg tcgttccatt
     4201 tgaagatgtc ggcgatgtag tcgaagagtc cgagcgttac gcctaaaaac gaggtggcga
     4261 tcgccatata ggaaaacagg gacaatattt tgtccatatt gccggtttgg gcgaatttgg
     4321 acagggtttc gatgaggacg gagacttgcc cttcggcggc gatgacgggg gcgaactcgt
     4381 tgcgcggcag gttgccttgg atggcggttt gccagaggac gtaaattacc agcgcaatca
     4441 gtgtgcccgt ccagatggat ttagccactt tgggcgcgtc gcctttaaag tatttgagca
     4501 ggctggagac gttgccgtgg aagccgaagg aagcgaggca gacgggcagg gcggtggcgg
     4561 cgtaaatcca gtagtttgtg ccggcggggg cttgggtatc gaagaggacg gacggcttgg
     4621 catcggcaat cagcccgccg gccgcccaaa taaaggtcaa taccatgccg ccgataagga
     4681 cgccggtgaa gcggtcgacc aagcgtgcgg atgcccatac gcaaaaggcg aggatgccga
     4741 agaagacgag ttgtccgacg gtgagtgaaa cgtcgccgcc tgccgcgctg cctaagcctt
     4801 tggcggtcag gtcgccgccg acgaagatat aagcgtaagt aagcaggtat aaaacgaagg
     4861 cgacggcgat gccgttgatg atgttccagc cgcgtccgag caggtctttg accatcgtgt
     4921 cgaaacttgc gccgtgcgga taatgggtgt tgacttccaa aatcatcagg ccgctggaaa
     4981 gcatagaaaa ccaggtgtac agcaacacgg ccagcgagcc ggtaaaccat acgccggatg
     5041 tggcggtcgg gttggcgagc atgcctgcgc cgatgaccgt gccggcgata atcatcgcgc
     5101 cgccgaacag tgaaggggtt ttgttgggca tattttgtct ttctgccaga aaaagcgagc
     5161 cgccattatg ccgtaaagtg taaggatttg taaggtattt gcgccgcgcc gcccgaaaag
     5221 gctttcagac ggcattgtgt tccatagtat aatcttgggt tttggagtgg gcggttcgtc
     5281 agatgggagg gaaaatgtcc gacaaaaaat ataatgtcga tgagggggaa atcgccaaat
     5341 tcagccggat tgccgacaaa tggtgggaca agtcgggcga gttcaaaacc ttgcacgaca
     5401 tcaatccgct gcggctggat tatatcgacg gacacgcgga tttgtgcggc aaacgggttt
     5461 tggacgtggg ctgcggcggc ggcatcttgg cggaaagtat ggcgcggcgc ggcgcggcgt
     5521 ttgtaaaggg catcgacatg gcggagcagt cgttggaaac cgcccgcctg cacgcggctt
     5581 tgaacaatgt cgccgatatc gaatacgaat gtatccgcgt ggaagacctt gccgaggcgg
     5641 aaccgcactc gttcgatgtg gtaacgtgca tggaaatgat ggaacacgtc cccgatcccg
     5701 ccgccatcgt gcgtgcttgt gccaatctgg tcaaaccgga cggcatggtg tttttttcca
     5761 ccatcaataa aaacccgaaa tcgtacctgc atctgattgt ggcggcggaa tatctgttga
     5821 agtttgtccc caaaggcacg cacgactgga aaaaattcat cgcacctgcc gagctggcgc
     5881 gaatgtgccg tcaggcaggc ttggatgtgg cggatacgaa gggtatgact taccatgtgt
     5941 tgtcgcaaac ttatgccctg tgcgattcga ccgatgtgaa ttatatgttt gcctgccgtc
     6001 cggcgttctg acggcgggtt tgcccgtttt tgagcaagtg agttgatatg tctgtctata
     6061 ccagtgtttc cgatgatgaa atgcgcggct tcctgagcgg ttacgatttg ggggaatttg
     6121 tttccctgca gggcatcgcg caggggatta ccaacagcaa ttattttctg acgacgactt
     6181 cgggacgtta tgtgctgacc gtgtttgaag tgttgaaaca ggaagagctg ccgttttttc
     6241 tggagcttaa ccggcatttg agtatgaagg gcgtggcggt tgccgcgccg gttgcgcgca
     6301 aagacggccg gcttgattcc gttttggcgg gtaagcctgc ctgcctagtt gcctgcctga
     6361 aaggttcgga taccgcgctg ccgacggctg agcagtgttt tcataccggt gcgatgttgg
     6421 cgaaaatgca ccttgccgcc gccgatttcc ctttggaaat ggaaaacccg cgttacaatg
     6481 cgtggtggac ggaggcgtgc gcccggctgc tgcccgtcct gtcgcaagac gatgccgcac
     6541 tgctgtgttc cgaaatcgat gcgttgaagg acaatctcgg caatcatctg ccttcgggca
     6601 tcatccatgc cgatctgttt aaagacaatg tgttgcttga cggcggtcag gtatcgggct
     6661 tcatcgattt ctattacgcc tgccggggca attttatgta tgacttggcg attgcggtca
     6721 acgattgggc aaggacggcg gacaataagt tggatgaggc gttgaaaaag gcgtttatcg
     6781 gcggttatga gggcgtgcgc cccttgagtg ccgaagaaaa ggcgtatttc ccgaccgccc
     6841 aacgtgccgg ctgcatccgt ttttgggtgt cgcgcctgtt ggattttcat tttccgcagg
     6901 cgggcgagat gacgtttatc aaagacccga acgcgttccg caacctgctg ttgagtttgg
     6961 gttgagtgcg tccggcgttt gacagaaatg ccgtctgaaa gggtttcaga cggcattttt
     7021 atggctgatt aaaacgaaaa tgagacgata gccgggtatt tt
//
LOCUS       DAWISC010000112         7041 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWISC010000112 DAWISC010000000
VERSION     DAWISC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.112"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WIW87_001518"
     CDS             complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WIW87_001518"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ5174660.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1370..3151)
                     /locus_tag="WIW87_001519"
     CDS             complement(1370..3151)
                     /locus_tag="WIW87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ5174661.1"
                     /translation="MAAGRVSRCRYILCDFRLFDDDDSSPRNVGGGGRFSLKAFYIRR
                     IKRILPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQ
                     EEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQFGFLAALCALSLAASFIPSALD
                     KYYLPHLRACELLIGSLTAVWMRCRQPAVGRRCAAVGALFAVCILSTCLFSYSEQTAY
                     FPGPAALIPCLAVAALIYFNHYEHPLKKFFQSKITVAAGLISYSLYLWHWPILAFMRY
                     IGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFAQSVLWIYALPMLILGAG
                     SFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHYKTF
                     FDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPRYSKVVLAM
                     RWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYILSS
                     RIPGYRQALRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPADFQIGGLPVYSDKD
                     HINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            3375..3623
                     /locus_tag="WIW87_001520"
     CDS             3375..3623
                     /locus_tag="WIW87_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174662.1"
                     /translation="MPSERSFRIGDRDIRNAGRLPVTAGQAVCFAGIGEGKSEMRVGV
                     YFDAAAFRRYGKRRKSSKSASDGISGKPPETCRIWVTR"
     gene            complement(3613..4704)
                     /gene="ychF"
                     /locus_tag="WIW87_001521"
     CDS             complement(3613..4704)
                     /gene="ychF"
                     /locus_tag="WIW87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ5174663.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(4819..6495)
                     /locus_tag="WIW87_001522"
     CDS             complement(4819..6495)
                     /locus_tag="WIW87_001522"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ5174664.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDADAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEDF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6670..>7041
                     /locus_tag="WIW87_001523"
     CDS             6670..>7041
                     /locus_tag="WIW87_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ5174665.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQ"
BASE COUNT         1564 a         1808 c         2006 g         1663 t
ORIGIN      
        1 cggcaatcgg attatttcca aaccaaaagc gcgtggttgc gtttgccgcg ccgaaggata
       61 gtgtatttgc cgaaacgttt gtgttcgccg ttcagcaggc aggcatcgtc ggggcgttcg
      121 gcggcgtggt tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg
      181 ttcacaaagc cgcgcgcttc tttattggag gatgccaaac cggtttttac caaggcttcg
      241 acgacattga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg
      301 aagtcgcttt cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgttgc
      361 gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt
      421 tgcgcttcgg gcttgctgcc gcttgccttg tctttggctt cgatggcatc gatttcttcg
      481 atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc
      541 cagaattggt agaactgata gggcgaggtt tttttcgcgt tcagccatac cgcgccgcct
      601 tcggttttgc cgaatttggt accgtctgat ttggttacca aaggcagggt cagaccgaat
      661 acttgttttt ggtgcaggcg gcgggtcagg tcgataccgg cggtgatatt gccccattgg
      721 tcggagccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtaa
      781 ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg
      841 cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg
      901 cgcaggaagt caaggcagtt catgctgccg aaccagtcgg cattgttcgc cataatggcg
      961 gcatttccgc cttcaaagct caagaaaggg gttaattggt tgcggatact ttccacccag
     1021 ccggcaacag tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg
     1081 atcataccgg tcgcgccgcc caccaaagca atcggcgtat gccccgcctg ttggaagcgg
     1141 cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag
     1201 ccgcaataaa gggcaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg
     1261 gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa
     1321 aaaaaattag cgtttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat
     1381 ggcgcgtatc gagaaaccgt tgtttttcgg aaaaacgctt tgccaattcc gtgccgccgt
     1441 aagggttgat gtggtctttg tccgagtaaa ccggcaatcc gccgatttga aaatctgcgg
     1501 ggatataggc ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga
     1561 tgcgtgcatt ggctttcagg gtgctttcgt cgtccgggcg cagggcttgg cggtaacccg
     1621 gtatgcgtga agacaagata taggcgcgct ggacgttgta agacgaggca aggttgtccg
     1681 ccatcaggta aacggcttgt ttttcggacg agagtttatg cagcatacgg tcgaattttt
     1741 ggaaaaaacc ggcatcatag gcaagggagc ggctgttttc gggcatttgg ctgccccagc
     1801 gcatcgccaa aaccactttt gaataccggg gcaggtgttc ttcggcatag cgataaacgg
     1861 cgcggcaggc tgcccagttt tggaacacac gggacgcgta gccttccaca taggcgcaag
     1921 cgtcggcgga aaccatagtg gcggaccatt tttctttttt gcccacggca tcgaagaatg
     1981 ttttgtaatg gtcggcgtgg gagtcgccca aaaccagcag ttccggctgt ttttccgtat
     2041 ccccccatag gcattgtttg ccggtattgt tgtggcagga ggtgttggaa cgcgtcagcc
     2101 ccaagcggtc gtattgcgcc ataaacggca gtctcatcgc aaaaaacgag cccgccccca
     2161 aaatgagcat aggcaaggca taaatccata aaacggattg tgcgaacgaa ccttgccatt
     2221 ttttaaacgg tttttcgatg cagtggtaag aaaacaggga aagcagcaat atcaggacga
     2281 ccgccgccgc cggcgaataa ggcggcaggt tgtccgggcc gatatagcgc ataaaggcca
     2341 atatcggcca atgccacaga taaagcgaat aggaaatcaa accggcggca acagtgattt
     2401 tcgattggaa aaatttttta agcgggtgtt cgtaatgatt gaaataaatc agcgcggcaa
     2461 cagccagaca gggaatcaaa gcggcggggc ccgggaaata ggcggtttgt tccgaatagg
     2521 aaaacaggca ggttgacaat atgcacacgg caaacaatgc gccgacggcg gcacagcgtc
     2581 tgccgacggc aggttgccgg cagcgcatcc acacggcggt cagcgatcct atcagtaatt
     2641 cgcaggcgcg caggtggggc aggtaatatt tatcgagcgc ggaaggtata aaggaggcgg
     2701 caaggcttaa ggcacacagt gcggcaagga agccgaactg tacgcgcagg cttttgcggg
     2761 cgacaagcag cagcagtatc ggaaagacaa agtaaaattg ttcttcgacc gacaaagacc
     2821 agatgtgcag caggggcttt tcttcctgcg cgggatcgaa ataatccttc ccccttgcaa
     2881 aatacaggtt agaggcgaaa cccaaggcgg tcagcgcgga tttccacaaa agaaagaaat
     2941 catctttggt gaataaaaag aagccgcctg ccagcgttgc cgccaatacg gcgaaaaatg
     3001 cgggcagaat ccgcttgatg cggcggatat aaaatgcctt cagggaaaac ctcccccccc
     3061 ccccgacatt tcgcggtgaa gaatcgtcgt catcaaaaag cctgaaatca caaagaatat
     3121 atcgacaccg agaaacccgc ccggcagcca atccttttcg atatggaaca cgatgacgga
     3181 caagacggcg gcggcgcgca atgtgtcgat gtccgggcgg tagggtaagg cttggctcat
     3241 aatgttttta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt gccgtactat
     3301 ctatactgtc tgcggtttcg tcgccttgtc ctgatttaaa gttaatccac tatactcgaa
     3361 acgcggcggc gcaaatgccg tctgaaaggt catttcgtat cggggatcgg gatattcgga
     3421 atgccggacg gcttcccgta acggcggggc aggcggtttg ttttgcagga atcggggagg
     3481 gcaaatcgga aatgcgggtg ggagtttatt ttgatgcggc tgcattccgg cggtacggga
     3541 aacgccgaaa atcatcaaaa tcggcttcag acggcatttc cggcaagccg cctgaaacct
     3601 gccgcatttg ggttacacgt taaacaaaaa gtgcatcaca tcgccgtctt gcacgacata
     3661 ttccttgcct tccacacgca ttttgccggc ttctttggct ttggcttcgc cgccgagcga
     3721 gacaaagtcg tcgtaagaaa tgacttgggc gcggatgaag ccgcgttcaa aatccgtatg
     3781 aatcacgccg gcggcttgcg gcgcggtgtc gcctttgtgt atcgtccacg cgcggacttc
     3841 tttcacaccg gcggtgaaat aggtttgcag ccccaagagg tcgtaaccgg cacgaatcag
     3901 gcggttcagg cccggttctt ccaagcccat ttcggcgagg aactcggctt tttcgtcgtc
     3961 ttccaattcg gcaatttcgc tctccatcgc ggcgcaaacg gcgacgacgg gggcgttttc
     4021 ttttgccgcc aattctttca ggcggtcgag gtgcggattg ttttcaaaac cgtcttcggc
     4081 gacgttgccc acatacatcg ccggtttggc ggtcagcagg aacagcggtt tgagcatcgc
     4141 gcgttcttcc gcgtccaaac cgaaggaacg cacgggtttg ccttcgtcca gatgcggcag
     4201 cagttttttg cacaaatcga ccagcttttg cgcgtctttg tcgcctgagc gggcgcgttt
     4261 ttcttcgcgg acgatggctt tttcgacact tgccaggtcg gcaagtgcca actctgtgcc
     4321 gatggtttca atgtcggcaa tcggatcgac gcggcctgca acgtggacga tgttgtcgtc
     4381 gtcaaagcag cgcacgacat tcacaatcgc atcggtttcg cggatgttgg caaggaactg
     4441 gttgcccaag ccctcgcctt tgctcgcgcc tgcaaccaaa ccggcaatat cgacaaattc
     4501 gacgatggca ggctgcattt tttgcggatt gacgattttt gccaattcgg ccatacgcgg
     4561 atcggggact tcgacgatgc cgacgttggg ttcgatggta cagaaaggat agtttgccgc
     4621 ttcgataccc gattgggtca gcgcgttaaa aagggtggat ttgccgacgt tgggcaaacc
     4681 gacgatgccg catttcaaac tcatgttttt tcctgaaaat agagaaattt aacggcggat
     4741 tatagcatac cgccgcccgc gttccgaaaa aatgccgtct gaaacggctt cagacggcat
     4801 ccggtttcag aaaaccgttc agaacaagcc gtgaatcacg ccttctgcgt ccacatcgat
     4861 tttctcggca gccggaactt tgggcaggcc gggcattttc atcatgttgc cgcacagggc
     4921 gacgatgaaa cctgcgcctg cggaaacggt gatgccgcgc acggcgatgc ggaagtcttc
     4981 ggggcagccc aacagtttgg cgttgtcgct caaagagtat tgggttttcg ccatgcagat
     5041 cggcattttg tccaagccca gtttttccag tgaagcgatt tcggcagacg cttccgcgct
     5101 gaaatcaaca tcttccgcgc cgtacacttt ttgggcaatc gcacggattt tgtctttgat
     5161 gcccaactcg acatcgtagg cgaaaccgaa gttattggtt tgactttcaa tggcgttgac
     5221 gactttgcgc gccaaatccg cgccgcccgc accacctttg ccccacactt cggtcaggga
     5281 aacttcaacg ccgtgttcgg cacaggcttt ttcaatcatc gccaactcgg catcggcgtc
     5341 ggacacgaag cggttgagcg caacgacgac gggcagtccg aatacgtttt tcaggttgga
     5401 aatgtgtttc agcaggttgg gcaaaccttt ttccaaagcg tctaaatttt cttcgccgag
     5461 gttggcgcgt tccacgccgc cgttatattt caacgcgcgg acagtcgcca cgacaacagc
     5521 cgcatcaggt ttcaaaccgg caaggcggca tttgatgtcg cagaattttt ccgcgcccaa
     5581 gtccgcgccg aagcctgctt cggttacggc gtaatcggca aggtgtttcg ccagacgggt
     5641 tgcggttacg gagttgcagc cgtgggcgat gttggcgaac gggccgccgt gtacgaaggc
     5701 gggcgtgcct tcgatggttt gcaccaagtt gggcttaatc gcatctttaa gcaatgccgc
     5761 catcgcgcca ttcgctttca aatctttggc gtaaacgggg ctgccgtctt tggcgtaggc
     5821 gacaaggatg ttgcccaaac gctctttcaa atcgctgatg tctttggcaa gacagaatac
     5881 cgccatcact tcggaagcaa cggtaatatc gaaaccgtca ggacgcatca cgccgtcaac
     5941 gggtttaccc atgccgtcga tgatgttgcg caactggcgg tcgttcatat cgaccacgcg
     6001 ccgccacagc acgcgtttgg ggtcgatgtt caactcgttg ccttggtaga tatggttgtc
     6061 gagcatcgcg gcaagcagat tatttgccgc accgatggcg tgaaaatctc cggtgaagtg
     6121 caggttgatg tcttccatcg gcaaaacttg ggcatagccg ccgcctgccg cgccgccttt
     6181 cacgccgaac accggcccca gagaaggttc gcgcagggca atcacggcat ctttgccgat
     6241 gtggcgcaac gcgtccgcca aaccgatggt tacggtggtt ttgccttcgc ccgccggagt
     6301 cgggttgatg gcggtaacca aaatcagcct gccctgtttt tgcggcagtt tgaacgcttc
     6361 ggcaggattg attttcgcct tgtaatgacc gtaaggctca atgttgtcgg cattcagacc
     6421 aagcttggcg gcaatttcgc caatcgggcg catggtggag gattgggcga tttcggcatc
     6481 ggttttgaag ctcatgattt tcctttagaa atgaggaggg acatgccgtc tgaaagcatc
     6541 aggcgacaaa caggtggatt gaaaataata tcaggcatat tataacgtta tccgcaccaa
     6601 acccgcagtg aaatttttga cgcagcaaca aaaataccgt tcatattgtt cacaatccaa
     6661 ggagaaaaca tgggcagcaa cgcatggctg ttttgggcat tggcatcggc aggcttcgcc
     6721 tcattgaccg ctattttcgc caaaatgggt ttacagggta tagattccga tttcgccacc
     6781 tttatccgca ccttggtcat ccttgccgct ttgttattgt ttttaaccta caccggcaaa
     6841 tggcagggtg tgaacggctt tacggggcgc aactggacat tcctcatcct atccggtctt
     6901 gctaccggcg catcttggct cgcctatttt aaagccctgc aactgggcaa cgcctcgcaa
     6961 gtcgccccca tcgacaaatt cagcctggtc ttggtcgcgc tgatggcggt ggttttcttg
     7021 gacgaacgcc cgaacacgca g
//
LOCUS       DAWISC010000113         6889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWISC010000113 DAWISC010000000
VERSION     DAWISC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.113"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(173..484)
                     /locus_tag="WIW87_001524"
     CDS             complement(173..484)
                     /locus_tag="WIW87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ5174666.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            1013..3292
                     /gene="nrdA"
                     /locus_tag="WIW87_001525"
     CDS             1013..3292
                     /gene="nrdA"
                     /locus_tag="WIW87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ5174667.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            3350..4363
                     /gene="dcm"
                     /locus_tag="WIW87_001526"
     CDS             3350..4363
                     /gene="dcm"
                     /locus_tag="WIW87_001526"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5174668.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            4356..5543
                     /locus_tag="WIW87_001527"
     CDS             4356..5543
                     /locus_tag="WIW87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ5174669.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            5604..6737
                     /gene="nrdB"
                     /locus_tag="WIW87_001528"
     CDS             5604..6737
                     /gene="nrdB"
                     /locus_tag="WIW87_001528"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ5174670.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT         2038 a         1453 c         1536 g         1862 t
ORIGIN      
        1 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 attaaggctg cggcaacggg aggacctcaa acgaaatggc gtaaggatag tttgttgtat
      121 ctatggaaaa tgccgtctga acctgtgttc agacggcata ttttatatgg gattaacggc
      181 ggaaactgcc gccgtcgaaa tcatcgaaag cctcataagc cgcccggcat tggattttct
      241 gttccgcata aggcagggag cggaatgctt tgcgcgcctt tttgttttcg cgcaattcct
      301 gcacgttgcc ttgcgatcgg taccacgcgg cacggcgttc caaatatttc ctgcattcgg
      361 ggtgcagcat ctgcttgttt gccttgcgga tatgtttggt atcgcggtgg gcgggttctg
      421 acgtaccggc agcggcagtt gtcgatacca gtcccaatat tgcagacaac agtatggcaa
      481 gcatcttttt gacagacatg acgatccttt cagtttgtca ttctttccag tataggatgg
      541 aaggatggaa cgggcaaatt gtcggcagtt tcagactcat tcagccgaca aaacttgccg
      601 tttgatatgc agattcaaat aacacattaa tctcaataat aaattccaat cggtaagaaa
      661 atggaatttg tcggcggcgg cgcggcaaaa tcatactttg caaaatttaa caatttgcag
      721 gggcagaaaa caggaagctt tcctttttcg tcggaaaatc cttatttcac cgccttgtag
      781 ccggagccgg tcaaaaggca aaaaatttac ccgtttttta tcggtaaaga attatcagat
      841 aaaacaaata ttataggaaa aatacgacag gcgggtttta tcgcgcattg cctgaaactg
      901 aaaaatacaa ccgttgtcaa gactggagaa aatgccaaaa atccactata ttgtctgcct
      961 taatttattt gaaaagactg tgtcttgaat atcaagagtg gaagaggaag cgatgaatac
     1021 accgactgat ttgaaagtaa ccaaacgaga cggaagatta gaagccattg atttggataa
     1081 gattcaccgt gtcgtcactt gggcggcgga cggattggaa aatgtttccg tgtcgcaggt
     1141 cgagttgaaa tcgcacatcc agttctacaa cggcatccgc accgacgaca tccacgaaac
     1201 catcatcaaa gccgctgccg atttaatttc ggaagatacc ccggactacc aataccttgc
     1261 cgcgcgtttg gcgattttcc atcttcgtaa aatagcctac ggcgagtacg agccgccgca
     1321 cctttacgac cacgttaaaa aacttaccga tgccggaaaa tacgacaggc atatccttga
     1381 ggattacagc cgcgaagaat ttgacgaact gaacgcctat atcgaccacg aacgcgatat
     1441 gtccttttcc tatgccgctg tcaaacagct cgaaggcaaa tatctggtac agaaccgcgt
     1501 tacccgccaa atttacgaaa cgccgcagtt tttatatgtt ttggtggcga tgtgcctttt
     1561 cagcaaatac ccgaaagagg cgcgcttggg ttacgtcaaa cggttttacg atgccgtttc
     1621 tacatttaaa gtatcgctgc cgactccgat tatgagcggc gtgcgtacgc ctacgcgcca
     1681 gttctcaagc tgtgtgctga ttgaatgcga cgatagtttg gattccatca atgccactac
     1741 cagcgcgatt gtgaaatacg tttcccagcg tgcgggcatc ggcatcaatg ccggacgtat
     1801 ccgcggtttg gacagcgaaa tccggggcgg cgaagcgcgg cataccggct gcattccctt
     1861 ctttaaaatg tttcaggcgg cggtcaaatc ctgttcgcaa ggcggcgtgc gcggcggcgc
     1921 ggcaaccttg ttctacccct tgtggcatat cgaagccgaa agcctgctgg tgttgaaaaa
     1981 caaccgcggt gtggaagaca accgtatccg tcagcttgat tacggcgtgc aaatcaaccg
     2041 cctgctgtac acccgcctga ttaagggcgg caacattacg ctgttttcgc ccaacgaggt
     2101 tccgggattg tacgaagcgt tttttgccga ccaagacgaa tttgagcggc tctatacgaa
     2161 atacgagcaa gaccctgata tccgcaagcg catcattccg gctgccgacc tgttttccac
     2221 gctgatgcag gagcgtgccg gaaccgggcg catctacatt caaaacgtcg atcactgcaa
     2281 tacgcacagc ccgttcgatc cgcgcgtcgc gcctgttcat cagtccaact tgtgtatgga
     2341 aatcgccctg ccgaccaaac cgctggacaa tatcaacgat ccgaacggcg aaatcgccct
     2401 gtgtacactg tctgccttta acttgggcgc attaaacagc ttggacgaat tggaagggct
     2461 tgccgacttg accgttcgtg cgctcgatgc acttttagat tatcagggat atccggtaga
     2521 agccgcgcgt acctctacta tgggccgccg ttcgctcggc atcggcgtga ttaactatgc
     2581 ttattatctg gcgaaaaacg gtgtccgcta cagcgacggt tccgcgctcg gtctgaccca
     2641 ccgtaccttt gaagccatac agtattacct gctcaaagca tcggcaaacc ttgccaaaga
     2701 atacggtgcg tgcacgctct ttaaccaaac cgtttattcg caaggcaaac tgcccatcga
     2761 cacttacaaa aaagatttgg atgccgtctg cggcgagcct ttgcattacg actgggaaag
     2821 cctgcgtgcc gaaatcgtca aatacggcct gcgcaactct actctgaccg cgctcatgcc
     2881 gtctgaaacc agctctcaaa tcgccaacgc caccaacggc atcgagccgc cgcgcggatt
     2941 ggtaacggtc aaagcatcga aagacggcat tttgaaacaa gtcgtgccgg agtttgaaac
     3001 cctgaaaaat gcctatgaaa ccctgtggca gcttcccggc aacgaaggct acctgaaact
     3061 tgtcggcgtg atgcaaaaat tcgtcgatca atcgatttcc gccaataccg cctacgaccc
     3121 gggcaaattc gaaggcggca aagtttctat gaaacaaatg ctcaaagacc tgctgaccgc
     3181 ctacaaatac ggcgtcaaaa ccctgtacta ccataacacc cgcgacggtg cggacgatac
     3241 gcagaccgat attcaggatg acggctgcgc gggcggggct tgtaagattt gatgaaaggg
     3301 ggagttttca gatggccttt agattaataa atcatctgaa atataaaata tgaaaaataa
     3361 aatacaaaat caattagata atatagttac cttaaataat gagaaattta cttttattga
     3421 tttatttgcg ggaataggtg gttttcgcat tgcaatggag aatgttggtg ggcgatgtgt
     3481 attttctagt gaatgggatg ataaagcccg tcaaacctac caagtaaatt ttaatgatat
     3541 tccttatgga gatattacat taaaggaaac caaagcagct attccaagta agtttgatgt
     3601 attaacagca ggattcccgt gtcagccatt ctctatagct ggtgtttcaa agaaaaaaag
     3661 cctaggacga gaaacaggct tcttagataa ggcgcaggga actctatttt ttgatgttgc
     3721 tgaaattatt ggaaaacatc gacctaaaat ttttctttta gagaatgtga aaaaccttgt
     3781 ttcgcatgac aaaggaaata catttaaagt aattaaaggg actttagaag agcttgacta
     3841 tcagatattt tatcaagtta tgaatgcaaa atattatgtt cctcaaaata gggagcgtat
     3901 ttttattgta ggttttgata gacaatattt taataaggaa ataaatttca attttccttc
     3961 cccaccagaa tcacaaccaa aattaaagca aattttggaa gatgatgtag ataattcttt
     4021 tactctttct gataacttat ggctttacct tcaaaattac gctaaaaaac ataaggcaaa
     4081 gggtaatgga tttggttttg gattagttga tttagatgga atatcacgaa ctctatctgc
     4141 acgatattac aaagatggtt ccgaaatact catccctcag aaaggaaaaa atcctaggaa
     4201 gctgacacct agggagtgct cgcgtttaat gggatttcct aaagattttg ttattgatgc
     4261 agtatccaag acagctgcat acaagcagtt tggcaattca attgctgtac cgttggttca
     4321 agctattgct aaacaaatta taaatgagtt aaaaaatgaa tgacttggca gctcagacaa
     4381 tacaagtagt aaataaatcc gatgacttag tagcttcagc aatacagaca gcaaataaat
     4441 caattgcagt atactgtcgt tatattcgtc ccaatgatgt tggtactact ggtagtcacc
     4501 aatcaggatt ttatattcaa aaacattttt cagacgacct ctttgatgta gtttgccaaa
     4561 agggaacaaa taaaactatt tcaatcaaaa ttaattggca agatggaagc gtcactaata
     4621 gcaattttaa atattacggc caaggcacga gaaatgaggc gagaattacg ggttttggca
     4681 agaattttga atttttaagt gataaatata gtggttcctt attggtattg tgcagagctt
     4741 gttataaaga tttattattc catgcttttg ttttatcttc agatgaagat attgaaatat
     4801 ttatcgcaga aactaacatt ttaccaggaa gtttatattt acctaaaaaa caagaagtag
     4861 aggataacct aacaaaatta ttttcactat ttcctaactt ccccaaaact gaagaaatgg
     4921 cagttttggc aagggaaaat attgcattaa ataaaactaa cgttagtaat gtgttaaaac
     4981 aatgggtttc aaaggaatat gaattatttt ccatttttga aaaacgtgaa tttgaaatta
     5041 ttaaatcaaa aattactgat ttggatagct tcattaattt tgcacattct tttacaaatc
     5101 gcagaaaagc aagagcagga aaatctttag agttacattt atcaagaata tttgacgaat
     5161 tttccttaaa atttgagacg caagcgaaga cagaagggaa aaagaaacca gattttttat
     5221 ttcctggaag tgaagagtac catgcgatgg atgaatcagg gaatttcata tttcaaactg
     5281 aaaagctgac aatgctaggt tcaaagacta cttgtaagga tcgctggcgc caagttttaa
     5341 atgaagcaga tcgaattcct cataagcatt tatttactct acaagaaggt atatctgata
     5401 ctcaaattca ggaaatgagt gatgagaatt taaccttagt tgtccctaaa gaatccgtaa
     5461 aaacatttgg aacttttggg aaaacccatg ttttaacttt agaaaatttt attaaatata
     5521 taaagtctca gcaggttagt taaaacttac agatcatttt taattaatat agagaataga
     5581 ttatgtcctg cgaacactta actatgtcat acagcacctt tcccaaaacc aaaaacgacg
     5641 cgctgaatga gccgatgttt tttggtcagc cggttaatgt tgcccgttat gaccagcaga
     5701 aatacgaagt atttgaaaaa ctgattgaaa aacaattgtc tttcttctgg cggcctgaag
     5761 aaatcgatgt ctcgcgcgac cgtatcgact acgccaatct gcccgaacac gaaaaacata
     5821 ttttcatcag caatctgaaa taccaaacac tgctcgattc catccaaggg cgcagtccga
     5881 atgttgcctt gctgcctttg gtttcgattc ccgagttgga aacgtggatt gaaacgtgga
     5941 gcttcagcga aaccatacac tcgcgcagct atacccacat catccgcaat attgtgaatg
     6001 atccgtcggt cgtgtttgat gatattgtcg aaaacgaata cattaccgcc cgcgccgaag
     6061 acattgcctg ctattacgat gacttaatcg aatacaccca gtattacaac ctgttgggcg
     6121 aaggggtgca caatgtcggc ggcaaacccg ttaccgtgtc tttgcgcggg ttgaagaaaa
     6181 aactctatct ctgcctgatg tgcgtcaacg tgttggaagc catccgtttc tacgtttcat
     6241 tcgcctgctc gtttgctttt gccgagcgcg agttgatgga aggcaacgcc aaaatcatca
     6301 aactgattgc ccgcgacgaa gccctgcacc tgaccggcac gcagcatatg cttaatctga
     6361 tgcgttctgg tgttgatgat tctgaaatgg cagaaattgc cgccgagttg caggacgaat
     6421 gtttccaact cttcaaaaaa gcggcggaac aggaaaaaga atgggcggca tatttgttta
     6481 aagacggttc gatgattggt ttgaacaaag aaatcttatc ccaatacgtc gaatatatta
     6541 ccaatctgcg tatgcaggcg gtggggttgc cggccggatt tgaaggcgca aatcaaaacc
     6601 cgattccgtg gattaatgcg tggctgtcgt ccgacaacgt acaggtcgcg ccgcaggaag
     6661 tggaaatatc ctcttatttg atcggtcaga tagattctga agtgaatacg gatgatttgg
     6721 gcgattttga gttgtaaatg ttttttgaaa aaatgccgtc tgaagccgga tgtttcagac
     6781 ggcatttgta tgttgtatga tgggaaaaac agcgggtagg atcggttatt gttcctttta
     6841 tagtggatta tagtggatta tagtggatta tagtggatta acaaaaatc
//
LOCUS       DAWISC010000114         6841 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWISC010000114 DAWISC010000000
VERSION     DAWISC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.114"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WIW87_001529"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WIW87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ5174671.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDTDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WIW87_001530"
     CDS             1555..1710
                     /locus_tag="WIW87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174672.1"
                     /translation="MKKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WIW87_001531"
     CDS             1707..2138
                     /locus_tag="WIW87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ5174673.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARCFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISAILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2272..6645
                     /gene="app"
                     /locus_tag="WIW87_001532"
     CDS             2272..6645
                     /gene="app"
                     /locus_tag="WIW87_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ5174674.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFTYKIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTKTDISGNVDLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     NLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFR
                     FMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFT
                     LQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALI
                     AAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATT
                     AFPRARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFA
                     EDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENT
                     FDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQ
                     ARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPA
                     QHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAA
                     AAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1832 a         1966 c         1668 g         1375 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccaccacg
       61 atgctttcga tccgtttatt ttccgataca aaacctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccaagc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac aacccttcgc tttcgccctg
      301 ccaaccggcc tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacaacgcg atgacggttt ccgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatacgctcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcaccgg cacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccaacgc
      721 gttcagcagg ctggacttgc ccacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccagtttg ccgcgtgcgt ctgccgcttc gagaaaatca atgtcttcct cgggaaaatc
      901 taacgtcgct tcgaccagca tccgcaaggt aatcaagtct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa agccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cttccgcctg tgccaagtcc agtttgtcgt tcaaaaacgc
     1081 acgcttcgta aactcgcccg gttcggcaag gcgcgcgccc aattccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcacccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgctg tcgattgcct gtccgtccgt
     1261 gtccgtaaaa tcagcatagg ttgcggtacg cggcttgggc gttttcccgc acaaagcctg
     1321 cgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgccctgg
     1381 tgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggta
     1501 taaaaccgca aaaatgcttg ataaatccgt ccgcctgacc taatatataa ccatatgaaa
     1561 aaacgaaaca catacgccct cctgctcggt ataggctcgc tgctgggtct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgaaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg tgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgcc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt gtttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ctgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagaaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taaaggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttgcataaat ttgtcacaga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttaac gacgataata atggcacagg aaaaatcaat
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcaagagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagcaa catcaatcaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa taacgaaact tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtaaacggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtacagtca ttttggatca gcaggcagac gataaaggca aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt taaacgggca ttcgctttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgccgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgactaag accgacatca gcggcaatgt cgatcttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcaacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcgg caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttcgcg ccgttcccta
     5101 ttatccgtta caccgccaac ttcggtagaa tcccgtttca acacgctgac ggtaaacggc
     5161 aaattgaacg gtcagggaac attccgcttt atgtcggaac tcttcggcta ccgcagcgac
     5221 aaattgaagc tggcggaaag ttccgaaggc acttacacct tggcggtcaa caataccggc
     5281 aacgaacctg caagcctcga acaattgacg gtagtggaag gaaaagacaa caaaccgctg
     5341 tccgaaaacc ttaatttcac cctgcaaaac gaacacgtcg atgccggcgc gtggcgttac
     5401 caactcatcc gcaaagacgg cgagttccgc ctgcataatc cggtcaaaga acaagagctt
     5461 tccgacaaac tcggcaaggc agaagccaaa aaacaggcgg aaaaagacaa cgcgcaaagc
     5521 cttgacgcgc tgattgcggc cgggcgcgat gccgtcgaaa agacagaaag cgttgccgaa
     5581 ccggcccggc aggcaggcgg ggaaaatgtc ggcattatgc aggcggagga agagaaaaaa
     5641 cgggtgcagg cggataaaga caccgccttg gcgaaacagc gcgaagcgga aacccggccg
     5701 gctaccaccg ccttcccccg cgcccgccgc gcccgccggg atttgccgca actgcaaccc
     5761 caaccgcagc cccaaccgca gcgcgacctg atcagccgtt atgccaatag cggtttgagt
     5821 gaattttccg ccacgctcaa cagcgttttc gccgtacagg acgaattaga ccgcgtattt
     5881 gccgaagacc gccgcaacgc cgtttggaca agcggcatcc gggacaccaa acactaccgt
     5941 tcgcaagatt tccgcgccta ccgccaacaa accgacctgc gccaaatcgg tatgcagaaa
     6001 aacctcggca gcgggcgcgt cggcatcctg ttttcgcaca accggaccga aaacaccttc
     6061 gacgacggca tcggcaactc ggcacggctt gcccacggcg ccgttttcgg gcaatacggc
     6121 atcgacaggt tctacatcgg catcagcgcg ggcgcgggtt ttagcagcgg cagcctttca
     6181 gacggcatcg gaggcaaaat ccgccgccgc gtgctgcatt acggcattca ggcacgatac
     6241 cgcgccggtt tcggcggatt cggcatcgaa ccgcacatcg gcgcaacgcg ctatttcgtc
     6301 caaaaagcgg attaccgcta cgaaaacgtc aatatcgcca cccccggcct tgcattcaac
     6361 cgctaccgcg cgggcattaa ggcagattat tcattcaaac cggcgcaaca catttccatc
     6421 acgccttatt tgagcctgtc ctataccgat gccgcttcgg gcaaagtccg aacacgcgtc
     6481 aataccgccg tattggctca ggatttcggc aaaacccgca gtgcggaatg gggcgtaaac
     6541 gccgaaatca aaggtttcac gctgtccctc cacgctgccg ccgccaaagg cccgcaactg
     6601 gaagcgcaac acagcgcggg catcaaatta ggctaccgct ggtaaccgcc ggatatgccg
     6661 aaaggggtct gacgatgcca ccgtgcgctg tcaaaccctt tttctgccgc cgccttgtgc
     6721 ctgctccatt gtctgataac cgtcaaattg atttttagcc catgtttggt gggtcgggga
     6781 aatctatatc ttcgtccgtg ccgaaatagt ctgagacctt tgcaaaattc ctttccctcc
     6841 c
//
LOCUS       DAWISC010000115         6766 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWISC010000115 DAWISC010000000
VERSION     DAWISC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.115"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(63..1355)
                     /locus_tag="WIW87_001533"
     CDS             complement(63..1355)
                     /locus_tag="WIW87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ5174675.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1433..2401
                     /locus_tag="WIW87_001534"
     CDS             1433..2401
                     /locus_tag="WIW87_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174676.1"
                     /translation="MKLTLAIPSLNLDEDEIRIPLCLHAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLVERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     ICRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     SDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2602..4068)
                     /locus_tag="WIW87_001535"
     CDS             complement(2602..4068)
                     /locus_tag="WIW87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5174677.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4310..6448)
                     /locus_tag="WIW87_001536"
                     /pseudo
     CDS             complement(4310..6448)
                     /locus_tag="WIW87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1441 a         2013 c         1722 g         1590 t
ORIGIN      
        1 tgccgtctga aaacctgttt gcaggttttc agacggcatt gttttcgtcg ccggttcaaa
       61 cttcaatccg attgcgacac ggtaaccggt atgccgcgca agcgcgcaag cagcgcgtcc
      121 gccttctgtt tctgcgcggc aaacgccgcc ttgtccgcct gcgtcaattc cggtgtcggc
      181 aatgcgaccg aaacaggatt gacgggctgc ccgttgatgc gcgcctcgta atgcaggtgc
      241 ggcccggtcg aacgcccggt cgaaccgaca aaaccgatga cctcgccgcc gcgcacattg
      301 ccttccgcct gcgaaaacgc gctcaagtgc gcgtacagcg tttccacacc gttggcgtgg
      361 cgtatcatca ccgcgttgcc gtatccgccc ttccggcctt taaaggtaat cacgccgtcg
      421 gcggaagccc tgaccggcgt tccctgcggt gcggcataat cgatgcccgt gtgcagccgc
      481 catgtgtgca ggatggggtg catacggtag ccgaacggcg aagaaatgcg cgtatagacc
      541 agcggctcga tgttgaagcc gcctttttcc tgcaacacct tgccgtcttc atcataataa
      601 ttgccgcccc ctccgccttc cttgtccgaa cggtaataga acgcctgatg ccttgtgccg
      661 cccttaacga cttcagccgc caaaatatcg cccgccgcca cctgctgccc gtggaaatac
      721 aggctgtcgt acatcaggcg cacggcatcg ccttccttca aaccgtcaag gctgaagcgg
      781 ccggcgaaaa tcccgcttaa ggattcgcgg atttcgacgg gcacttccgc ccgcgccagc
      841 gaaccgcgcg ccgacgtttt gaccacgacc gaacgcagcg tcggcaaaac cttcatatcc
      901 gcctcagaag ccgaccgccg ccatatgccg cctttctttt ccaaagcgac cagattgcgc
      961 tcgccgtctt cgtcggtaaa aaactgcact tcgcgcgcgc cgccgtcgcc gccgaccaaa
     1021 acatgaaccg actggtcggc acgcaaatgc cgcaaatcgg cttcgccgcc atatttttcc
     1081 gtgattcggg caatctcgtc ccgcgccata cccgaacgcg ccagcacgtc cgccagcgag
     1141 tcgcccggct gcaccgcctc ctgcacccaa tatgccgtct gaacgccgct gccgccccaa
     1201 gacagcggcg gcagattttg ttccacgcgc tgcggtctga cgcgctccgt cctctctgtc
     1261 gaagcaatgt atgccgccga caccaaaata atcgaaacgg caagcgcacg cagcgcgtat
     1321 ttccgatgtt ttgccgaaag tgggaagaca gccatattgt tcctttttga cggcgtttgc
     1381 gccgaatcct ctataatgtt gcgcctgacc tgcatttctg aaacgtcaaa cgatgaaact
     1441 caccctcgcc attccctcgc tcaatttgga tgaagacgaa atccgaatac ctctatgcct
     1501 gcacgctttc aacaaaatcc tgcaatacgg ctcgctgcac cgccaaagct gcacggcttc
     1561 ggcattttac gcgcgttatt tgtggtgcgg cagactggtg gaacgcgcgg cgcaaagcct
     1621 gaatatgccg tctgaagccg tcgcgctcgc cacgcccgtc tggcaaaaga tggggatgca
     1681 tcaggcaaat gtgctaacgg cggaatatct gaacgtcggt acggatgaag cggaacggat
     1741 ctgccgcgac ctgtcggcgt tttacggcga cattccatgg cgtttcgttc ccgtcctgcc
     1801 cgaattatgg ctggtttccc ttccttgcgc gtaccgctgg ggcgcgaagc ccgttttgga
     1861 cttgggcgga ttgttgggcg ctgacgatca gccggacgga gaagatgcgt tggaatggct
     1921 gcgcgtccaa accgaaatcc aaatgtggct ggcggcgcac cccgtcaacc ataaccgcaa
     1981 gaaacgcggg ctgcccgagt tgaacggctt atggctttgg gacggcacaa acggcggcgc
     2041 gcaaggcggc acgcttttcg ccgatacggt ttggagccgt ttccactccg accgccgcgc
     2101 attacccgac aacttccgcg cctatgaaga aacggcggca cacctgcccg acacgcacca
     2161 tatcctgttt atggacgatt tgcgcctgac cgccctgaca ggggacaggg agcggtatgc
     2221 ggcaatattg cagcagtggg aagaacgctg gttcgcacca atctacgaag ccgtccgcac
     2281 cggcaaaatc aaacggctgg acatcaccac ggacggacaa cacggcggta cgctgacctt
     2341 caaacccgcc gacaggtgga aattttggcg cggcgcgaaa acctttgacg gaatctggtg
     2401 aacccgcccg ccgcaaccgc gacaatgccg tctgaagccc gaatcgggtt tcagacggca
     2461 ttttacctcg catcgccgtt atgccggaaa aaccggaatg cgtttccgca cacgcggcgg
     2521 cacgcaaagc accgcccgaa agccgccgat gcccgccgtg ataccgccgc cggcagggtt
     2581 tcctccgaaa aggaacagca atcagaacgt tttattaaac tcgacaaacg cccgattttt
     2641 ctcgtattcg ttgaacacat cgttactccg cgtttcgcgg tgcgacaacg tcaggcgcgg
     2701 cgtgatgcct ttgaaatgca atgcccggtg ccaaaggctc aaggatgtgt tcaattcttt
     2761 atccctgcgc ctttcccctt taaaaccgct gaaaaagccg ggtttttcat aatgccgttt
     2821 cgccgcgccg aggcgcaaca gcgaagacag gccgctgccg ccccattcct gcccccaggc
     2881 aaagcgcagg ccgtaacggt tgaaattgtc gccccggtcg gcggggttgc gctcgcggta
     2941 aaaatccaaa ccgcccatcc aatattggcg cgcattccgg taaaacacca gcgaattgga
     3001 aatttgcaaa tgggtattgt cggaacgcgc ccggcgcgta ttcttcaaac gcccccactc
     3061 cgccgaagac aacgtttgcc atttcggggt ttgccaacgg ttgaaataaa ggcgtgcgcc
     3121 gttggtgtaa gaataagcgt cgttgccgta ggtgcggcgt tcgtggaaca ctgccagccc
     3181 ggcatctttg cgccggtcgg caaaaccgat gccgccggaa acgcctgccg tcatatcgtt
     3241 gaatttctta ttccccggat aaaccctgcc ggacacgtcg ccgcccgccg tcgtgtacca
     3301 gccgtttttc agcgaccatt ttttctccgc gccgagccgg taattgaccg ccgtgccgtc
     3361 cacctgtttc gggaaagtcc atttgccgta ctgctgccgt ttcggggctt ggttgatatt
     3421 gtgttcgcgg gtgacgctga agccgccatt taccttccac gcatcgcgtt cgcgcaatgc
     3481 cttgcggtac agctcgacct gctccatcag ctgcggcggc aggttttccg ccttcaggcg
     3541 gtcgaactgg tctgccgccg cctcgttctg cctgttttca aacaatgctg ccgccaaacg
     3601 catacggacg gcgggcgcgt cgggttgggc ggcaatcaat tcccggtaat gggaaatcgc
     3661 ctccttcacc ctaccgtctg cctgcgccaa aatcccttgt gcataaagtg ccaacatctt
     3721 atcctgctgc gcctgttgta ggtaaatcgg caaaataacg cggataccgg caatattgtt
     3781 tgagaccact gcggaataca tcgcgcggga caacaattcg ggatttttca gcagggtttc
     3841 gccgtcaatc tgcaaaactt ttcctttttc ccgcacctgc cccggcacct tctccctgtc
     3901 gatcggtttg acctccgctt catgaagcct gaactcggga cggcttctca aatccggttc
     3961 gcgcggtgtt tcttcggcat atgccgcgct tgccaataaa ggcagcagca gcatccatct
     4021 gtttcgtgca ggcataaatg tcttcccaca aaaataaaaa ataaccatta tcaatcggcg
     4081 gattgtatca gttttttatg ccgctgttcc aaaccctgcc gcccgatgcc gcgacaatgc
     4141 cgtctgaagc cagaatcggg tttcaaacgg cggcaaaaaa ccggcggttt tccgccggtt
     4201 tcatcattat gccccgccct tcggggcggc agggttcgga ttggtttgcg gggcggcgcg
     4261 cggtgccgtg ccggtgtacc gcctgtgcgt caaaacattt cccgacgcat caaaacagcc
     4321 atttgagcga caatgcggct tctttgtcgc cgtccgtcct tttgccatat ccgatgcgtg
     4381 cggacatatg gcctttccaa ccggcttcaa taccgaaccg cccttcgagt gccgtcctgc
     4441 ctgccagcgt ctgtttttcg ccgtccattt ccacgccgaa agattttgac ctgtgcaaaa
     4501 cattaaaagc ggcaaaaggc tgaagattga caccgttacg caaagcaaaa cgggtttttg
     4561 cccgaatgcc ggcgcggctt tgccactgac cgctgccgag cagtccgacc gccgtcccct
     4621 cgctgtcggt aaagccgccg tttacgccca agtaggtaaa ctgcgcctgc ggttgtaggt
     4681 aaaaccgcac attattgcct tttccgacaa tgccttccgc cacaagcgcg ttgtagccgc
     4741 cttcgacaga agccgtccaa cctttggttt tgtagcgttc cgcacggttt tcatcattga
     4801 tgcggtgttt gaaacgttgg tattgcaacc agccgtccaa atacgcaccc gtttgtttat
     4861 cgcgcaactg atgccacgca gcataaacac ccccgccata accatacaaa tcactgcctg
     4921 ccgcaccgcc tttgccgttg actgatgcgt gctggccggc cctgccgccc atcacgccga
     4981 ttgccagtcg gctgccttca ttttgccgta caaacacctc gccgccgatt tgcacgcctt
     5041 tgcgccaccc gtccgcagcc gcgccgcccc gtatattttg atgcgaccgg ccgccgatga
     5101 agcgcagcca caatttttga cgggacggat cggcggcata cacgtcgtcg cccctgtcgg
     5161 cggcacgcag cccaaacaaa gtatttgccg cataagcctg ttgggcatat aaaaccgctt
     5221 ccgcctcggg gatagccaaa cgctgcacgc ccaagcaaaa tccgccttcg cattgcttga
     5281 gcaataattc gtacagtccc tgccggtaat tttgtttaaa cgcaaacgaa ccggaatccg
     5341 ccgtttttgc cgcaatcagt ttgcgcccgt cgagcgtttt cagattttcc ggccggcgtt
     5401 ccaaaaccac gccggattgt ccgctgactt tgtcttcaaa ggtcagggcg ggttcgtccg
     5461 actcccgcac gtcgtagccg aacaggaaat ccgcgccgcc cgcataatcc ttatggacgg
     5521 tcagtttttc atctttcgtg ttcaggcgga tgcgggcatt atcggaagtt tccagcgtat
     5581 ggatgtcgga atggcggcgc ggttcccaaa gggaatcttt cagcgtcatc ttggtcagtt
     5641 ccaacacctg tccggcgacg tgggatttgt tttcgatttt gacgtgtaat tttttattga
     5701 agatgttgtt gagactagtc tgtgatgtat tactatgagg agtgagtacg gaatcttcta
     5761 ccaatttatt aattaaatcg ggcatcgtct gtttgagcgt actaaatttt gttccaaact
     5821 gtgctatata cgcctcctca gtccgttttt tattttctgc ccaagcttcc ggtcttgttt
     5881 tgtataaatc ctgtaattga cttttaaccg cctgctttaa atcagcccca tacttctctg
     5941 taatatattt tttgacaacg ggcatattat cgacggcgag gatgccgcta ttgtaatttt
     6001 taccttggat attgatttcg tcatacccat attcggatga attttgatcg gttttaccgc
     6061 tggaaaaata atgggaagtg ccgggtttcg gcttgtctga aaatgaaaaa tcggcatcgt
     6121 cgccgatttt ggtagaacga gtatttgaac caagctgagc tctgaacata ccgaagcaat
     6181 caaaagatat gctgcacggg ccgcgccaaa cgtgggaatt tctgtcttcc gccgcttcta
     6241 aaataaattt gtttttaatc tcggcttctt tgacgggatc gtacgcatcc gaacctcaaa
     6301 catatggcgt gcaaaacagc gataaaacag ataatgttaa tagattaacc cgaccgcttg
     6361 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg
     6421 cttgcttgct ttcagtgtca tgagaaattt tcctttgtca agtgttaaaa ttataatgat
     6481 tatatactac attatactac atcgcaatga aaaatcagga aaagcaaaaa accctccgcc
     6541 gtcattcccg cgaaagcggg aatctagaat tttaatgccg caagaattta tcaaggatgg
     6601 tcggggttta aaggtctgga ttcccgcctg cgcgggaatg acggtttaga aatcacccga
     6661 aacaagaaaa agaaccgaaa ccgaacaagc cggattcccg cctgcgcggg aatgacggga
     6721 tttcaggtta ctgtttttga ttttctgttt tgtggaaata acggga
//
LOCUS       DAWISC010000116         6628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWISC010000116 DAWISC010000000
VERSION     DAWISC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.116"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WIW87_001537"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WIW87_001537"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ5174678.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1510
                     /locus_tag="WIW87_001538"
                     /pseudo
     CDS             1102..1510
                     /locus_tag="WIW87_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
     gene            1531..3594
                     /locus_tag="WIW87_001539"
     CDS             1531..3594
                     /locus_tag="WIW87_001539"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ5174679.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQIGKPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLVVLHGGDIVNVSVLGLQSGNKTLGHRFLSDGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTVLEGQARRLNATAAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLTNVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEITEAVEQHYQPRFAGDNLPEGKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFN
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3611..4657
                     /locus_tag="WIW87_001540"
     CDS             3611..4657
                     /locus_tag="WIW87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ5174680.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIAQRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     GGKYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4699..5526
                     /locus_tag="WIW87_001541"
     CDS             4699..5526
                     /locus_tag="WIW87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ5174681.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDTSFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5538..5633
                     /locus_tag="WIW87_001542"
     CDS             5538..5633
                     /locus_tag="WIW87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ5174682.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5675..6517
                     /locus_tag="WIW87_001543"
     CDS             5675..6517
                     /locus_tag="WIW87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ5174683.1"
                     /translation="MQNHVISLASAAERRAHIAATFGVRGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1699 a         1779 c         1732 g         1418 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacttgcaag gtgtagaaaa cgtctacgac ctcgtttggg
      661 caaaaacgct cgacggcaac accgtcacct acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct tgtcctcaaa gcggggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaacgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggctttg aaatggcggg ggggcgattg
     1321 cggcgatttt tcgtgcctgt ttcaaaaatc cggtgcccga agcttgggag aaaccgagaa
     1381 aaaagccatt atctcttggc tggcagaccg cgaagatgtt gccactttgt acgccacccc
     1441 gctggttgac ggtttttatg gggatgacgg ctgggaaaat tacagcgaaa cctataaaca
     1501 ggcagattaa ccaaattcaa agaaagccga atgatgaccc aaaccctttt aatcgaactc
     1561 cttaccgaag aactcccgcc caaagccctg aataatctgg gcaaccattt cgccgcttcc
     1621 gttgccgaag gcttggaaaa agcgcaactg gttgacggcg cagccgaatt tacggcttat
     1681 gcctcgccgc gccgtttggc ggttcaagtc aaaaacgtca aagccgttca ggctgatcaa
     1741 aaaattgtga aaaaaggccc tgccgtagcg aatgccatga aagacggtgc gccaaccaag
     1801 gctttggaag gtttcgcacg cggtgcgggc gcgaaaatcg aagacttgac catcgtccac
     1861 gatggcaagc aggacgtgta cgcctacgaa tacgtccaaa tcggcaaacc gttgggcgga
     1921 cttttggaag acattatcaa tcaagcggtt aagaagctgc cgattcccaa agtaatgcgt
     1981 tggggcagca gcacgtttac ctttgtgcgc cccgttcacg ggctagttgt actgcacggc
     2041 ggcgacattg tgaacgtcag cgttttgggt ctgcaaagcg gcaacaaaac cttgggacac
     2101 cgcttcctct ccgacggcga aattaccatt gaaaacgccg acagctacgc cgcacaaatg
     2161 cgcgagcaag gcaaagtcgt cgcttcgttt gccgagcgca aagccgcgat tcagacggta
     2221 ttggaagggc aggcacgccg tctgaacgcg accgccgccg ccgatgaagc cttgttggac
     2281 gaagtaaccg cgctggtcga atggcctgtg gtattggaag ccggttttga agaacacttc
     2341 ctcgccgtac cgcaagaatg cctgattctg acgatgcagc aaaaccaaaa atacttcccg
     2401 ctgctcgacc aaaacggcaa gctgatgaac cgcttcctgc tggtgtccaa tctgcaaacc
     2461 gaagatccgt cacacatcat ccaaggcaat gaacgtgtct tgcgcgcgcg cctgtctgat
     2521 gccgagttct tctacaaaca agaccaaaaa gcgactttgg aaagccgcct gcccaaactg
     2581 acgaacgtgg tttatcacaa caaaatcggt tcgcaagccg aacgcatcga acgcctgcaa
     2641 agcatcgccg cccatatcgc caaggctttg ggcgcggatg ccgccgcagc cgaacgcgcc
     2701 gcgcgtttgg caaaagccga cttagtaacc gaaatggtcg gcgagttccc cgaactgcaa
     2761 ggcacgatgg gcaaatacta cgcccgcttg gacggcgaaa ccgaagaaat taccgaagcc
     2821 gtcgagcaac actaccaacc gcgttttgcc ggcgacaacc tgcctgaggg caaaattgcc
     2881 gccgccgttg cgcttgccga caaactggaa accttggtcg gcatttgggg tatcggcctg
     2941 attccgaccg gcgacaaaga cccatacgcc ctgcgccgcg ccgcattggg tattttgcgt
     3001 atgctgatgc agtacggttt ggacgtaaac gaactgattc agacggcctt caacagcttc
     3061 ccgcaaggtt tgctcaacga aaaaacgccg tctgaaaccg ccgacttcat gcaggcgcgc
     3121 cttgccgtat tgctgcaaaa cgattatccg caagacatcg ttgccgccgt acttgccaag
     3181 cagccgcgcc gtttggacga tttgaccgcc aaactgcagg ctgtcgccgc gttcaaacaa
     3241 ctgcccgaag ccgccgcgct cgccgctgcc aacaaacgcg tgcaaaacct gctgaaaaaa
     3301 gccgatgccg agttgggcgc ggttaacgaa agcttgctgc aacaggacga agaaaaagcc
     3361 ctctttgccg ccgcacaagg cttgcagccg aaaattgccg ccgccgttgc cgaaggcaat
     3421 ttccagaccg ccttgtccga actggcttcc gtcaaaccgc aagtcgatgc cttcttcgac
     3481 ggcgtgatgg tgatggcgga agatgccgcc gtgaaacaaa accgcctgaa cctgctgaac
     3541 cgcttggcag aacaaatgaa cgcggtagcg gatatcgcgc ttttgggcga gtaaccgttg
     3601 tacagtccaa atgccgtctg aagccttcag acggcatcgt gcctatcggg agaataaatt
     3661 gcagccttta gtcagcgtat tgatttgcgc ctacaacgta gaaaaatatt tcgcccaatc
     3721 attagccgcc gtcgtgaatc aaacttggcg caacttggat attttgattg tcgatgacgg
     3781 ctcgacggac ggtacgcttg ccattgccca gcgtttccaa gaacaggacg gccgcatccg
     3841 catcctcgcg cagccgcgca attccggtct gattccctct ttaaacatcg ggctggatga
     3901 attggcaaag tcgggggggg ggaaatatat tgcgcgcacc gatgcagacg atattgccgc
     3961 ccccgactgg attgagaaaa tcgtgggtga gatggaaaaa gaccgcagca tcatcgcgat
     4021 gggtgcgtgg ctggaagttt tgtcggaaga aaaggacggc aaccggctgg cgcggcacca
     4081 cgaacacggc aaaatttgga aaaaaccgac ccgacacgaa gacattgccg actttttccc
     4141 tttcggcaac cccatacaca acaacacgat gattatgagg cgcagcgtca ttgacggcgg
     4201 tttgcgttac aacaccgagc gggattgggc ggaagattac caattttggt acgatgtcag
     4261 caaattgggc aggctggctt attatcccga agccttggtc aaataccgcc ttcacgccaa
     4321 tcaggtttca tccaaataca gcatccgcca acacgaaatc gcgcaaggca tccaaaaaac
     4381 cgccagaaac gattttttgc agtctatggg ttttaaaacc cggttcgaca gccttgaata
     4441 ccgccaaata aaagcagtag cgtatgaatt gctggagaaa catttgccgg aagaagattt
     4501 tgaacgcgcc cgccggtttt tgtaccaatg cttcaaacgg acggacacgc tgcccgccgg
     4561 cgcctggctg gattttgcgg cagacggcag gatgcggcgg ctgtttacct tgaggcaata
     4621 cttcggcatt ttgcaccgat tgctgaaaaa ccgttgaaaa acgccgcttt atccaacaga
     4681 caaaaaacag gataaattat gcaaaaccac gttatcagct tagcttccgc cgcagaacgc
     4741 agggcgcaca ttgccgatac cttcggcagg cacggcatcc cgtttcagtt tttcgacgca
     4801 ctgatgccgt ctgaaaggct ggaacaggca atggcggaac tcgtccccgg cttgtcggcg
     4861 cacccctatt tgagcggagt ggaaaaagcc tgctttatga gccacgccgt attgtggaag
     4921 caggcattgg acgaaggtct gccgtatatc accgtatttg aggacgacgt tttactcggc
     4981 gaaggtgcgg aaaaattcct tgccgaagac gcttggctgc aagaacgctt tgacccggat
     5041 acctccttta tcgtccgctt ggaaacgatg tttatgcacg tcctgacctc gccctccggc
     5101 gtggcggatt actgcgggcg cgcctttccg ctgttggaaa gcgaacactg ggggacggcg
     5161 ggctatatca tttcccgaaa agcgatgcgg tttttcctgg acaggtttgc cgccctgccg
     5221 cccgaagggc tgcaccccgt cgatctgatg atgttcagcg attttttcga cagggaagga
     5281 atgccggttt gccagctcaa tcccgccttg tgcgcccaag agctgcatta tgccaagttt
     5341 cacgaccaaa acagcgcatt gggcagcctg atcgaacacg accgcctcct gaaccgcaaa
     5401 cagcaaaggc gcgattcccc cgccaacaca ttcaaacacc gcctgatccg cgccttgacc
     5461 aaaatcagca gggaaaggga aaaacgccgg caaaggcgcg aacagttcat tgtgcctttc
     5521 caataaaagg aggagagatg gacatcgtat ttgcagcaga cggcaggatg cggcggctgt
     5581 ttaccttgag gcaatacttc ggcattttgc gccgattgct gaaaaaccgt tgaaaaacgc
     5641 cgctttatcc aacagacaaa aaacaggata aattatgcaa aaccatgtta tcagcttagc
     5701 ttccgccgca gaacgcaggg cgcacattgc cgcaaccttc ggcgtgcgcg gcatcccgtt
     5761 tcagtttttc gacgcactga tgccgtctga agaactgaat cggatgatgg cggaattggt
     5821 gccgggattg gcaaaacagc acctgttgag cgaagtggaa aaagcctgct ttatgagcca
     5881 cgccgtattg tggaaacagg cattggatga aggtctgccc tatgttgccg tatttgagga
     5941 tgatgtcctg cttggcaaag acgcagaaaa gttccttgcc gaagatactt ggttggaaga
     6001 gcgttttgat aaggattctg cctttatcgt tcgtttggaa acgatgtttg cgaaagttat
     6061 tgtcagaccg gataaagtcc tgaattatga gaatcggtca tttcctttgc tggagagcga
     6121 acattggggg acggctggct atatcatttc gcgtgaggcg atgcggtttt tcttggaaag
     6181 gtttgccgtt ttgccggcag agtggattaa agcggtagat tggatgatgt ttacttattt
     6241 ctttgataag gaggggatgc ctgtttatca ggttaatccc gccttatgta cccaagaatt
     6301 gcattatgcc aagttcctca gtaaaaacag tatgttgggt agcgatttgg aaaaagatag
     6361 ggaacaagaa agaagacacc gccgttcgtt gaaggtgatg tttgacttga agcgtgcttt
     6421 gggtaaattc ggtagggaaa agaagaaaag aatggagcgt caaaggcagg cggagcttga
     6481 gaaagcttac ggcaggcggg tcatatcgtt caaatagttt gtgtaaaata tagcggatta
     6541 aaattagaaa tggatacact gtcattcccg cgcaggcggg aatctagtcc gttcggtttc
     6601 gcttgtttta agtttcgggt aacttcca
//
LOCUS       DAWISC010000117         6466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWISC010000117 DAWISC010000000
VERSION     DAWISC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.117"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(90..1886)
                     /locus_tag="WIW87_001544"
     CDS             complement(90..1886)
                     /locus_tag="WIW87_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ5174684.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIVLQKSGQVPPYNEWLAAS
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPDNLLNQVWTNRPALPAETVFIH
                     DPDYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGIAVEPYAQVADKLAQIGGVLLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1942..2283
                     /locus_tag="WIW87_001545"
     CDS             1942..2283
                     /locus_tag="WIW87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174685.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2344..3597
                     /locus_tag="WIW87_001546"
     CDS             2344..3597
                     /locus_tag="WIW87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ5174686.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WIW87_001547"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WIW87_001547"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ5174687.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WIW87_001548"
     CDS             complement(5340..5528)
                     /locus_tag="WIW87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ5174688.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWIGQLESE"
     gene            complement(5532..6410)
                     /locus_tag="WIW87_001549"
     CDS             complement(5532..6410)
                     /locus_tag="WIW87_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5174689.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
BASE COUNT         1404 a         1678 c         1851 g         1533 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtccat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttcg gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcttttt tgttcggcac tcggcgtgcc gacggggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcaaaagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggtgtcgcgc tgtaatgcgg cagtgcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaaa tgaagcctgg gcgcacgctg
      841 cggtggcgat aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga tgggttgatt
     1021 ccctcgataa ggcgcacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggctcgatga gcagcacgcc gccgatttgc gcgagtttgt cggcaacttg ggcgtaaggt
     1141 tcgaccgcga tgccggcggt ttgcagcgcg gcggcggctt cggcgttcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcag aaacatagtc ggggtcgtgg atgaacaccg tttcggcggg gagggcgggg
     1441 cggtttgtcc acacttgatt cagtaaatta tccgggtgtt cgatgcggat gtttttggcg
     1501 gcgagtgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gcttgccgcg agccattcgt tgtacggcgg cacttgcccg
     1621 cttttttgca gcacaatgcc gctgcccgca agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc acacgcccgc ttcatcggtg gtcaggacaa acgtgccgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcatccaag ccttgcgcct tcatggcttc gcgtaatgcg
     1861 gacagataat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 tggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt ttatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgggcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cagttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggtccgggg
     2401 atcatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagtccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggcgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ctggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggttaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gtcgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaacct tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agttttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctacg
     4201 cccgctgccc gacaaacaca aaggtttgag cgatcaggaa accaaatacc gccaacgtta
     4261 cgtcgatttg attgccaacg aagaatcgcg caataccttc atcaaacgca gccaaatcat
     4321 ccaatccgtg cgcaatttta tggtgggcga gcattatctt gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccatcaca atgccttaga
     4441 tattccgctt tacctgcgaa tcgcccccga gctgtatctg aaacgcttgg tggtcggcgg
     4501 cttggaacgc gtgtttgaaa tcaaccgtag cttccgcaac gaaggcatgt ccgtgcgcca
     4561 caatcccgaa ttcaccatga ttgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcagatggcg gaagacatca tccgcaacgc atcgcgcaca gtaaacggca cggcaaaaat
     4681 cagctacaac ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccatcctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaacttgcc ctgtttgaag gttgcgccga gggcaagctg tggaacccga cctttattgt
     4921 cgattacccg gtcgaagttt ccccgttggc acgcgcttcc gataccaaac aaggtctgac
     4981 cgaacgcttt gagcttttcg ttgtcggccg cgaactggca aacggctatt ccgagttgaa
     5041 cgaccccgaa gaccaagccg aacgcttcaa agcgcaagtg gcgcaaaaag acgcgggcga
     5101 cgacgaagcg atgcactacg atgccgacta catccgcgcg atggaattcg gtttgccgcc
     5161 gacaggcggc tgcggcatcg gcatcgaccg cctggtaatg ctgctgaccg attcgcaaac
     5221 catccgcgat gtgattctgt ttccgcagat gcgtcctgag taatcataaa aacagttgaa
     5281 atagaaatgc cgtctgaacc cgattgggtt cagacggcat tttgtatggc agtacggatt
     5341 tattcgcttt ccaactgacc gatccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttctcgcctg tgcggcggac
     5581 ataaaccgtt ttttgacctc cagcaccaca cctaaaatcg tgctgccttc gggcaggtca
     5641 aaggcttcag acggcctgcc cgtctgataa atcagcatcc tgcccgcaat atcgataaca
     5701 aggtgcgcga cgaccagccg cttgtcggac agttcaatca gcacgatgtc ctcgtccgca
     5761 gggcgcatac gcggctcgac caccaccaaa tccccttttt cgataaccgg cgacatggac
     5821 gtgtcgggca tggcaacggc aaacgtgtac tgcgaggggg cgaccaccga agcaaacaag
     5881 tcgaaactgc ctgtgagttt gcctgtacgg gcgaaagccg ccgcctgacg gtctgacaac
     5941 agggggcgtg gtgtgagcct catcgacagt gctttgccgt cggcatcgct gtggttgtgc
     6001 gtatgcgtat gatggttgtt gatggtcgta tcgccttgga catcgttaag gactatgctg
     6061 tttgaacctg ccacggattt ggtcatctca ttgtaggctt gcgtcgtgat gcgctcttgc
     6121 ttggtcatac ctgttacagc ctgtttcagc atttctaacg ctatgtgatt cttcctaagg
     6181 gtcgctatac cggcttttgc agcttgctca ttaccacaag aggggcaacc ccagctactt
     6241 cctatgaaca tattggagta cctcgaacag gtttggtttc catgtttagg gcagttgatt
     6301 acacacggct tggctgtacc ctcaaactcg accaacttaa tgccacgatg accgaaacgt
     6361 tcctcaacct cagcagcagc ttcttcaaat gtttttgacc ttcttgccat attgacctcc
     6421 ggattattga ttattttggc tatgctttaa cgagggattg gaatga
//
LOCUS       DAWISC010000118         6457 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWISC010000118 DAWISC010000000
VERSION     DAWISC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.118"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            284..2131
                     /locus_tag="WIW87_001550"
     CDS             284..2131
                     /locus_tag="WIW87_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ5174690.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVLIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTQAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGMPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVR
                     DRAGIDARLGAEFLAEGRKIPGSAVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2186..6355
                     /locus_tag="WIW87_001551"
     CDS             2186..6355
                     /locus_tag="WIW87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ5174691.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1509 a         2074 c         1704 g         1170 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtagtgcc ggacaggctt tgctttatca ttcggcgcga
       61 cggtttaatt tattgaacga aaataaattt atttaatcct gcctattttc cgacactatt
      121 ccgaaacgca gcctgttttc catatgcgga ttagaaacaa aataccttaa aacaagcaga
      181 tacatttccg gcgggccgca acctccgaaa taccggcggc agtatgccgt ctgaagcgtc
      241 ccgccccgtc cgaacagtgt taaaatcgaa agccgccaca ccgatgcacg acacccgtac
      301 catgatgatc aaaccgaccg ccctgctcct gccggcttta tttttctttc cgcacgcata
      361 cgcgcctgcc gccgaccttt ccgaaaacaa ggcggcgggt ttcgcattgt tcaaaaacaa
      421 aagccccgac accgaatcag tcaaattaaa acccaaattc cccgtcctca tcgacacgca
      481 ggacagtgaa atcaaagata tggtcgaaga acacctgccg ctcatcacgc agcagcagga
      541 agaagtattg gacaaggaac agacgggctt cctcgccgaa gaagcgccgg acaacgttaa
      601 aacgatgctc cgcagcaaag gctatttcag cagcaaagtc agcctgacgg aaaaagacgg
      661 agcttatacg gtacacatca caccgggccc gcgcaccaaa atcgccaacg tcggcgtcgc
      721 catcctcggc gacatccttt cagacggcaa cctcgccgaa tactaccgca acgcgctgga
      781 aaactggcag cagccggtag gcagcgattt cgatcaggac agttgggaaa acagcaaaac
      841 ttccgtcctc ggcgcggtaa cgcgcaaagc ctacccgctt gccaagctcg gcaatacgca
      901 ggcggccgtc aaccccgata ccgccaccgc cgatttgaac gtcgtcgtgg acagcggccg
      961 ccccatcgcc ttcggcgact ttgaaatcac cggcacacag cgttaccccg aacaaatcgt
     1021 ctccggcctt gcgcgtttcc agcccggtat gccgtacgac ctcgacctgc tgctcgactt
     1081 ccaacaggcg ctcgaacaaa acgggcatta ttccggcgcg tccgtacaag ccgacttcga
     1141 ccgcctccaa ggcgaccgcg tccccgtcaa agtcagcgta accgaggtca aacgccacaa
     1201 actcgaaacc ggcatccgcc tcgattcgga atacggtttg ggcggcaaaa tcgcctacga
     1261 ctattacaac ctcttcaaca aaggctatat cggttcggtc gtctgggata tggacaaata
     1321 cgaaaccacg cttgccgccg gcatcagcca gccgcgcaac tatcggggca actactggac
     1381 aagcaacgtt tcctacaacc gttcgaccac ccaaaacctc gaaaaacgcg ccttctccgg
     1441 cggcgtctgg tatgtgcgcg accgcgcggg catcgatgcc aggctggggg cggaatttct
     1501 cgcagaaggc cggaaaatcc ccggctcggc tgtcgatttg ggcaacagcc acgccacgat
     1561 gctgaccgcc tcttggaaac gccagctgct caacaacgtg ctgcatcccg aaaacggcca
     1621 ttacctcgac ggcaaaatcg gtacgacttt gggcacattc ctgtcctcca ccgcgctgat
     1681 ccgcacctct gcccgtgcag gttatttctt cacgcccgaa aacaaaaaac tcggcacgtt
     1741 catcatacgc ggacaagcgg gttacaccgt tgcccgcgac aatgccgacg ttccttcagg
     1801 gctgatgttc cgcagcggcg gcgcgtcttc cgtgcgcggt tacgaactcg acagcatcgg
     1861 acttgccggc ccgaacggat cggtcctgcc cgaacgcgcc ctcctggtgg gcagcctgga
     1921 ataccaactg ccgtttacgc gcaccctttc cggcgcggtg ttccacgata tgggcgatgc
     1981 cgccgccaat ttcaaacgta tgaagctgaa acacggttcg ggactgggcg tgcgctggtt
     2041 cagcccgctt gcgccgtttt ccttcgacat cgcctacggg cacagcgata agaaaatccg
     2101 ctggcacatc agcttgggaa cgcgcttcta aaccgatatg gccacttcag acggcattgc
     2161 agcaaaccat tttgaaacag acattatgac cgataccgca ccgacagata ccgatccgac
     2221 cgaaaacggc acgcgcaaaa tgccgtctga acaccgccct accccgccgg caaaaaaacg
     2281 ccgcccgttg ctgaagctgt cggcggcact gctgtctgtc ctgattttgg cagtatgttt
     2341 cctcggctgg ctcgccggta cggaagcagg tttgcgcttc gggctgtacc aaatcccgtc
     2401 ttggttcggc gtaaacattt cctcccaaaa cctcaaaggc acgctgctcg acggcttcga
     2461 cggcgacaac tggtcgatag aaaccgaggg ggcagacctt aaaatcagcc gcttccgctt
     2521 cgcgtggaaa ccgtccgaac tgatgcgccg cagcctgcac attaccgaaa tttccgccgg
     2581 cgacatcgcc atcgttacca aaccgactcc gcctaaagaa gaacgcccgc cgctcagcct
     2641 tcccgacagc atagacctgc ctgccgccgt ctatctcgac cgcttcgaga cgggcaaaat
     2701 cagcatgggc aaagcctttg acaaacaaac cgtctatctc gaacggctgg atgcttcata
     2761 ccgttacgac cgcaaaggac accgccttga cctgaaggcc gccgacacgc cgtggagcag
     2821 ttcgtcgggg gcggcctcgg tcggcttgaa aaaaccgttt gccctcgata ccgccattta
     2881 caccaaaggc ggactcgaag gcaaaaccat acacagtacg gctcggctga gcggcagcct
     2941 gaaggatgtg cgcgccgaac tggcgatcga cggcggcaat atccgcctct cgggaaaatc
     3001 cgtcatccac ccgtttgccg aatcattgga taaaacattg gaagaagtac tggtcaaagg
     3061 gttcaacatc aatccggccg ccttcgtgcc ttccctgccc gatgccggac tgaatttcga
     3121 cctgaccgcc atcccgtcgt tttcagacgg catcgcgctg gaaggttcgc tcgatttgga
     3181 aaacaccaaa gccggctttg ccgaccgcaa cggcatcccc gtccgtcagg ttttaggcgg
     3241 ctttgtcatc cggcaggacg gcacggtgca tatcggcaat acgtccgccg ccctgctcgg
     3301 acggggcggc atcaggctgt cgggcaaaat cgacaccgaa aaagacatcc tcgatttaaa
     3361 tataggcatc aactccgtcg gcgcggaaga cgtactgcaa accgcgttca aaggcaggtt
     3421 ggacggcagc atcggcatcg gtggcacgac cgcctcgccc aaaatctctt ggcaactcgg
     3481 catcggcacg gcgcgcacgg acggcagcct cgccattgca agcgacccag caaacggaca
     3541 gcggaaactg gtgctcgaca ccgtcaacat cgccgccggg caaggcagcc tgaccgcgca
     3601 aggctatctc gagctgttta aagaccgcct gctcaagctg gacatccgtt cccgcgcatt
     3661 cgacccttcg cgcatcgatc cgcaacttcc ggcaggcaat atcaacggct caataaacct
     3721 tgccggcgaa ctggcaaaag agaaattcac aggcaaaatg cggtttttac ccggcacgtt
     3781 caacggcgta ccgattgccg gcagtgccga cattgtttac gagtcccgcc accttccgcg
     3841 tgccgccgtc gatttgcggc tggggcggaa cattattaaa acagacggcg gcttcggcaa
     3901 aaaaggcgac cggcttaacc tcaatatcac cgcacccgat ttatcccgtt tcggtttcgg
     3961 actcgcgggg tctttaaatg tacgcggaca cctttccggt gatttggacg gcggcatccg
     4021 aacctttgaa accgaccttt ccggcgcggc gcgcaacctg cacatcggca aggcggcaga
     4081 catccgttcg ctcgatttca cgctcaaagg ttcgcccgac acaagccgcc cgatacgcgc
     4141 cgacatcaaa ggcagccgcc tttcgctgtc gggcggagcg gcggttgtcg ataccgccga
     4201 cctgatgctg gacggcacgg gcgtgcagca ccgcatccgc acacacgccg ccatgacgct
     4261 ggatggcaaa ccgttcaaat tcgatttgga cgcttcaggc ggcatcaaca gggaacttac
     4321 ccgatggaaa ggcagcatcg gcatcctcga catcggcggc gcattcaacc tcaagctgca
     4381 aaaccgtatg acgctcgaag ccggtgcgga acgcgtggcg gcaagtgcgg caaattggca
     4441 ggcaatgggc ggcagcctca acctgcaaca cttttcttgg gataaaaaaa ccggcatatc
     4501 ggcaaaaggc ggcgcacacg gtctgcatat cgccgagttg cacaatttct tcaaaccgcc
     4561 cttcgaacac aatctggttt taaacggcga ctgggatgtc gcctacgggc gcaacgcgcg
     4621 cggctacctc aatatcagcc ggcaaagcgg cgatgccgta ttgcccggcg ggcaggcttt
     4681 gggtttgaac gcattttccc tgaaaacgcg ctttcaaaac gaccgcatcg gaatcctgct
     4741 tgacggcggc gcgcgtttcg ggcggattaa cgccgatttg ggcatcgcca acgccttcgg
     4801 cggcaatatg gcaaatgcac cgctcggcgg caggattacc gcctcccttc ccgacttggg
     4861 cgcattgaag ccctttctgc ccgccgccgc gcaaaacatt accggcagcc tgaatgccgc
     4921 cgcgcaaatc ggcggacggg taggctctcc gtccgtcaat gccgccgtca acggcagcag
     4981 caactacggg aaaatcaacg gcaacatcac cgtcgggcaa agccgctctt tcgataccgc
     5041 gcctttgggc ggcaggctca acctgaccgt tgccgatgcc gaagtattcc gcaacttcct
     5101 accggtcgga caaaccgtca aaggcagcct gaatgccgcc gtaaccctcg gcggcagcat
     5161 cgccgatccg cacttgggcg gcagcatcaa cggcgacaaa ctctattacc gcaaccaaac
     5221 ccaaggcatc atcttggaca acggctcgct gcgttcgcat atcgcgggca ggaaatgggt
     5281 aatcgacagc ctgaaattcc ggcacgaagg gacggcggaa ctctccggta cggtcggtat
     5341 ggaaaacagc ggacccgatg tcgatatcgg cgcggtgttc gacaaatacc gcatcctgtc
     5401 ccgccccaac cgccgcctga cggtttccgg caacacccgc ctgcgctatt cgccgcaaaa
     5461 aggcatatcc gttaccggga tgattaaaac ggatcagggg ctgttcggtt cgcaaaaatc
     5521 ctcgatgccg tccgtcggcg acgatgtcgt cgtattaggc gaagtcaaaa aagaggcggc
     5581 ggcaccgctc cccgtcaata tgaacctgac tttagacctc aatgacggca tccgcttcgc
     5641 cggctacggc gcggacgtta ccataggcgg caaactgacc ctgaccgccc aatcgggcgg
     5701 aagcgtacgg ggcgtgggca cggtccgcgt catcaaaggg cgttataagg catacgggca
     5761 ggatttggac attaccaaag gcacggtctc ctttgtcggc ccgctcaacg atcccaacct
     5821 caacatccgc gccgaacgcc gcctttcccc cgtcggtgcg ggcgtggaaa tattgggcag
     5881 cctcaacagc ccgcgcatta cgctgacggc aaacgaaccg atgagtgaaa aagacaagct
     5941 ctcttggctc atcctcaacc gcgccggcag cggcagcagc ggcgacaatg ccgccctgtc
     6001 tgcagccgca ggtgcgctgc ttgccgggca aatcaacgac cgcatcgggc tggtggatga
     6061 tttgggcttt accagcaagc gcagccgcaa cgcgcaaacc ggcgaactca accccgccga
     6121 acaggtgctg accgtcggca aacaactgac cggcaaactc tacatcggct acgaatacag
     6181 catctccagc gcggaacagt ccgtcaaact gatttaccgg ctgacccgcg ccatacaggc
     6241 ggttgcccgt atcggcagcc gttcgtcggg cggcgagctg acatacacca tacgtttcga
     6301 ccgcttctcc ggttcggaca aaaaagactc cgccggaaac ggcaaaggaa aataagcggt
     6361 tttcagacgg cgcgccgcca aaccggacat ttgaaaacct gcttttccac cgtccgccgc
     6421 cgccgtccgc ctgcaaggga acagaatcga tatagtg
//
LOCUS       DAWISC010000119         6440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWISC010000119 DAWISC010000000
VERSION     DAWISC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.119"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            143..1684
                     /locus_tag="WIW87_001552"
     CDS             143..1684
                     /locus_tag="WIW87_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ5174692.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     QTETL"
     gene            1713..2312
                     /locus_tag="WIW87_001553"
     CDS             1713..2312
                     /locus_tag="WIW87_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ5174693.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2309..2914
                     /gene="nadD"
                     /locus_tag="WIW87_001554"
     CDS             2309..2914
                     /gene="nadD"
                     /locus_tag="WIW87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ5174694.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2973..3359
                     /gene="rsfS"
                     /locus_tag="WIW87_001555"
     CDS             2973..3359
                     /gene="rsfS"
                     /locus_tag="WIW87_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ5174695.1"
                     /translation="MNEQELQDLQKMVGVAVNALGDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     CDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3413..3883
                     /gene="rlmH"
                     /locus_tag="WIW87_001556"
     CDS             3413..3883
                     /gene="rlmH"
                     /locus_tag="WIW87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ5174696.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3956..4222
                     /locus_tag="WIW87_001557"
     CDS             3956..4222
                     /locus_tag="WIW87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ5174697.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4379..5062
                     /locus_tag="WIW87_001558"
     CDS             4379..5062
                     /locus_tag="WIW87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ5174698.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5186..5698
                     /gene="coaD"
                     /locus_tag="WIW87_001559"
     CDS             5186..5698
                     /gene="coaD"
                     /locus_tag="WIW87_001559"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ5174699.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5730..6386
                     /locus_tag="WIW87_001560"
     CDS             5730..6386
                     /locus_tag="WIW87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ5174700.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1398 a         1697 c         1862 g         1483 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tatttcagac ggcgggagga aggcggatac
       61 ggtatgtagt cggattgaaa tatacggtat gtgggggctt ttgggtacaa tcgcaacatc
      121 agtgtttcaa gtggggaagg cgatggatgg acggcgttgg gtggtatggg gtgcttttgc
      181 cctgctgcct tcggcttttt tggcggtaat ggtcgttgcg cctttgtggg cggtggcggc
      241 gtatgacggt ttggcgtggc gcgcggtgct gtcggatgcc tatatgctca aacgtttggc
      301 gtggacggta tttcaggcag cggcaacctg tgtgctggtg ctgcctttgg gcgtgcctgt
      361 cgcgtgggtg ctggcgcggc tggcgtttcc ggggcgggct ttggtgctgc gcctgctgat
      421 gctgcctttt gtgatgccca cgttggtggc gggcgtgggc gtgctggccc tgttcggggc
      481 ggacgggctg ttgtggcgcg gcaggcagga tacgccgtat ctgttgttgt acggcaatgt
      541 gtttttcaac cttcctgtgt tggtcagggc ggcgtatcag gggtttgtgc aagtgcctgc
      601 ggcacggctt cagacggcac ggacgttggg cgcgggggcg tggcggcggt tttgggacat
      661 tgaaatgccc gttttgcgcc cgtggcttgc cggcggcgtg tgccttgtct ttctgtattg
      721 tttttccggg ttcgggctgg cgctgctgct gggcggcagc cgttatgcca cggtcgaagt
      781 ggaaatttac cagttggtca tgttcgaact cgatatggcg gttgcttcgg tgctggtgtg
      841 gctggtgttg ggggtaacgg cggcggcagg gttgctgtat gcgtggttcg gcaggcgcgc
      901 ggtttcggat aaggcggttt cccctgtgat gccgtcgccg ccgcagtcgg tcggggaata
      961 tgtgctgctg gcgtttgcgg cggcggtgtt gtctgtgtgc tgcctgtttc ctttgttggc
     1021 aattgttgtg aaagcgtggt cggccggcga atcgtggcgt gtgttaatgg aaagtgaaac
     1081 gtggcaggcg gtgtggaata ctttgcgctt ctcggcggcg gcggtgtatg cggcggcggt
     1141 tttgggtgtg gtgtatgcgg cggcggcgcg gcggtcggcg tggatgcgcg ggctgatgtt
     1201 tttgccgttt atggtgtcgc cggtttgtgt ttcggcgggc gtgctgctgc tttatccgca
     1261 gtggacggct tcgttgccgt tgctgctggc gatgtatgcg ctgctggcgt atccgtttgt
     1321 ggcaaaagat gttttatcag cctgggatgc actgccgccg gattacggca gggcggcggc
     1381 gggtttgggt gcaaacggct ttcagacggc atgccgcatc acgttccccc tcttgaaacc
     1441 ggcgttgcgg cgcggtctga ctttggcggc ggcaacctgc gtgggcgaat ttgcggcgac
     1501 attgtttctg tcgcgtccgg aatggcagac gctgacgact ttgatttatg cctatttggg
     1561 acgcgcgggt gaggataatt acgcgcgggc gatggtgctg acattgctgt tggcggcgtt
     1621 cgcgctgggt attttcctgc tgttggacgg cggcgaaggc ggaaaacaga cggaaacgtt
     1681 ataatgtgaa ccctttttca gaggacggca aaatgagcga gttggataac atccttgccc
     1741 ataaccggca gtttgtcgag tcgggcgaat atgaaaaata ctttaccgac aaataccccg
     1801 aacgcgggct ggcagttttg tcctgtatgg atgcgcggat tatcgggctg ctgcccgacg
     1861 cgttgggttt gaaaaacggc gatgccaagc tgattaaaaa tgccggcgcg ctggttacgc
     1921 acccgtgggg ttcggtgatg cggagccttt tggttgccgt gtttgaactg aaggtcagag
     1981 agattatggt catcgcccat cacgattgcg gtatgcaggg gctgaatgcc gaagaattcc
     2041 tcgggcgcgt ccgggaaagc cggattcccg aagaccgtat cgaaaccctg cgttatgccg
     2101 gtatcgacct cgacggctgg ctgaccggtt tcgacaacgt cgaagacagc gtgcgccaca
     2161 cggtggacct tatccgtaac catccgctga tgccgcgcca tatcgccgtt cacggactgg
     2221 tcatccatcc cgttaccggc aaactgacgc tggttgtgga cggcagtgtt tcagacggca
     2281 tggacttatc ggaaggaatg gaaacatcat gaagaaaatc ggattgttcg gcggtacttt
     2341 cgacccgata cacaacggac atcttcatat cgcccgtgcc tttgccgacg aaatcggctt
     2401 ggacgcggtt gttttcctgc cgacaggcgg cccgtatcac aaagacgcag cctccgcttc
     2461 cgccgccgac cgccttgcca tggtcgaatt ggcgacggca gaagacgcgc gttttgccgt
     2521 cagcgattgc gacatcgtcc gagaaggtgc aacctatact tttgataccg tccaaatctt
     2581 ccgccagcag ttcccatccg cgcaactctg gtggctgatg ggcagcgaca gcctgatgaa
     2641 gctgcacaca tggaaaaaat ggcagatgct cgtgcgcgaa accaatatcg ccgtcgccat
     2701 gaggcagggc gacagcctgc accaaacccc gcgcgaactg cacgcgtggc tgggcaagtc
     2761 ccttcaggac ggcagcgtcc gcatcttgtc cgccccgatg cataatgtgt cgtcaacgga
     2821 aatccgccgc aaccttgccg gccaaggcgt ttcagacggc atcccgcctg ccgccgcacg
     2881 ctacatccgc gaacacggtt tgtatgaaaa ataaagtcaa atcagtaaga aacggctata
     2941 atgccgtctg aaaacatccc gttaggaaaa taatgaacga acaagaactg caagacctgc
     3001 aaaaaatggt cggggtcgcc gtcaacgccc tcggagacat caaagccaaa gacatttccg
     3061 ttctcgaaac ccaagacaaa acttcgctgt ttgccagaat gattatcgcc agcggcgaca
     3121 gtacgcgcca agtcaaagca ctggccaaca acgttgccgt cgatttgaaa gaagccggtt
     3181 ttgaaatcct cagtaccgaa ggcgacagcg gcgaatggac gctggttgat gcaggagacc
     3241 tcgtcgtcca cgtcatgctc cctgccgtgc gcgacttctg cgacatcgac accctctggg
     3301 gcggcgagaa accgagtttc cacgccggaa tgcagaagcc gtggcacgcg gcagactgat
     3361 tcccgatgcc gtctgaacct tcagacggca ttttcctgta agggagaaag cattgaacat
     3421 caccgttttg gcagtcggca ccaaaatgcc gcgctgggtt gatgaggccg tcgccgaata
     3481 cgccaaacgc ttcggacgcg acgtcgccta cgcactcaaa gaaatcaaac ccgaaaaacg
     3541 cggcgcgggc gtgaatgccg cccaaggtat ggcggcggaa gaaaaacgca tccttgaagc
     3601 cattccgcaa ggcgcgttcc tcgtcgttct tgacgaacgc ggcaaagcac cgacctccgt
     3661 cgagctggcg gaacacctca aaagctggcg gcaaaacggc gaacacgtct gcttcgtcat
     3721 cggcggcgcg gacggcatga ccgaccgcct caaacagcag gcacgcatga tgatgcgcct
     3781 gtccagcctg accctgccgc acggcatggt gcgcgtcttt ctgaccgagc agctctaccg
     3841 cgccgtttcc atcctgcaca accatcccta tcatcgggaa taagaaggct tttgtccgta
     3901 tccctaatcg gttaaaatca ccccgttatt tctgaacaca gcaaaggaat ccgctatggc
     3961 acgcatggta ttctgcgtca agctcaacaa agaagccgaa ggcatgaaat ttccgccgct
     4021 tcccaacgaa ttgggcaagc gcatttttga aaacgtatcg caagaagcgt gggcggcgtg
     4081 gacgcgccac caaacgatgc tgattaacga aaaccgttta agcctcgccg atccgcgcgc
     4141 gcgcgaatac ctggctcagc agatggagca gtatttcttc ggcgacggcg cggatgccgt
     4201 tcagggatac gttccgcaat aacggttttc cgttttgaac acaggctgtc cgaaactgct
     4261 tcagacggcc tttaaaatac gccggacaac tttatcttca cgcaaccgga caaattttga
     4321 cattgggcaa tgttaaaatc ccccattatt cccaaccgcg ctttcaggag cagatgatat
     4381 gcaacacgac gtttacgact acaccgcgca tacggtttct aaaaacaccg tcctgcagaa
     4441 aacctaccgc ctgctcggat tttcatttat tccggcttcc gcaggcgcgg cacttgccgc
     4501 caatgccggt ttcaattttt acgccgcctt cggttcgcgc tggatagggt ttgccgtcgt
     4561 gttggcgttt ttctacggta tgatccactt catcgagaaa aaccgttaca gcaataccgg
     4621 cgttaccctg ctgatggtat tcacattcgg tatgggcgta ttgatcggcc ccgtgctgca
     4681 atacgcgctc catattgccg acggtgcgaa aatcgtcggc attgccgccg cgatgaccgc
     4741 cgccgtcttt ttaacgatgt ccgccttggc gcgccgaacc cggctcgata tgaacgcgct
     4801 cggacgcttc ctgaccgtag gtgcggtcat tctgatggtc gccgtggttg ccaatctgtt
     4861 tttgggtatt cccgcactcg ccctgaccat ttccgccggt tttgtcttgt tcagttcctt
     4921 aatgattatg tggcaggtac gcaccgtcat cgacggcggc gaagacagcc acatcagcgc
     4981 ggcactgaca ctgtttatct cgctttacaa catcttcagc agcctgctga acatcctgtt
     5041 gtctttaaac ggcgaggatt gatgccggta aaacgccgtc cgactatgcc gtctgaaaat
     5101 gcttcagacg gcattttact ttgggcatac aatacggatg cacacgcacc aacacacagc
     5161 cgactatgaa gccgggagga atcaaatgtt accgaataca ccgcgccgcg ccgtttatgc
     5221 cggcagtttc gatccgccca cattggggca tctgtggatg atacggcagg cgcaatctat
     5281 gtttgacgaa ctcatcgtcg ccatcggcat taaccccgac aaacgcagca cctataccgt
     5341 cgctgaaagg caggatatgt tgtgcgatat tactaaaatg tttcccaacg tcagaaccga
     5401 tgtatttgaa aaccgatttc tggtgcatta cgcccgtgag gtagatgcag gattcatcgt
     5461 gcgcggcatc cgttctgctt cggattacga atacgaacgt tccatgcgcc atatcaacag
     5521 cgacctcgcc cccgaaatat ccaccgtatt cctcatgccg ccgcgcgaaa tcgccgaagt
     5581 gtcgtccact atggtcaaag gactggtcgg gccggaaggc tggacggaaa cgatccaccg
     5641 ctacgtgccg caagctgtgt acgaaaaaat ccttgccgaa catcaacacg aaaattgaac
     5701 ccttctattt ttgcaaggca tatatggata tgttggaaat tttgttccgc catcaagact
     5761 tcgtcgccat caacaaaccg ggcggcatat ccgtccacca agacagcggc gagaccggac
     5821 tggcccgaac actcgccata cagttgggcg tagagcgtgt gtggctgctg caccgattgg
     5881 acaaacagac cagcggcatt ttactgtttg ccctcaacaa agaaagcgcg tccgccctta
     5941 gcggacagtt tgccggcaaa agcatcaaaa aaacctattt ggcactgtcc gaccgcaagc
     6001 cgtccaaaaa gcagggttgg attaaaggcg gaatggaaaa atccagatgt ggaatgtgga
     6061 agttgacgcg caatacagaa aatatcgccg ttacccgatt ccacagcatc agtatcgccg
     6121 aaaaactgcg gctgttcatc ctagaaccgc atacgggcaa aacgcaccaa ttgagagtgg
     6181 cgatgaaaag tttgggcagt ccgattctgg gcgacagctt atacggcgga acagaatccg
     6241 aaaccatgtt tctgtatgca tggaaaatac aatttgacta ccaaaaccga caaatcgaaa
     6301 ttgttgcacc cttaaaaaac gaatggcaga ctgaaaatat ccatcgggca ctggaagaat
     6361 tctgtatgga aaataaaatt gactgatata aattaaattt ataaacaaat agataaatat
     6421 ttttaaaaaa atttcttcag
//
LOCUS       DAWISC010000120         6311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWISC010000120 DAWISC010000000
VERSION     DAWISC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.120"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            61..444
                     /locus_tag="WIW87_001561"
     CDS             61..444
                     /locus_tag="WIW87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ5174701.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            737..1210
                     /locus_tag="WIW87_001562"
     CDS             737..1210
                     /locus_tag="WIW87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ5174702.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1336..2439
                     /gene="mnmA"
                     /locus_tag="WIW87_001563"
     CDS             1336..2439
                     /gene="mnmA"
                     /locus_tag="WIW87_001563"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ5174703.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2510..4180
                     /locus_tag="WIW87_001564"
     CDS             2510..4180
                     /locus_tag="WIW87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ5174704.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4292..5926
                     /locus_tag="WIW87_001565"
     CDS             4292..5926
                     /locus_tag="WIW87_001565"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ5174705.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESTDLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            6154..>6311
                     /locus_tag="WIW87_001566"
     CDS             6154..>6311
                     /locus_tag="WIW87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ5174706.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQLIEQYLNRQKTR
                     YLRDHRGRP"
BASE COUNT         1660 a         1703 c         1584 g         1364 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 ctcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcta catcaaattt ccaggcagta tttgtatgta
      541 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
      601 aaccgatcca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
      661 caacgctgta ctggtttttg ttaatccgct ataagattaa gcacattccc cgtctgatat
      721 tataaggagc agaaagatga aaaaattatt ggcggccgtg atgatggcag gtttggcagg
      781 cgcggtttcc gccgccggag tccacgttga ggacggctgg gcgcgcacca ccgtcgaagg
      841 tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac gaagccaaac aagacttttt
      901 gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg catacccaca tcaacgacaa
      961 cggcgtgatg cggatgcgcg aagtcgaagg cggcgtgcct ttggaagcga aatccgttac
     1021 cgaactcaaa cccggcagct atcatgtgat gtttatgggt ttgaaaaaac aattaaaaga
     1081 gggcgataaa attcccgtta ccctgaaatt taaaaacgcc aaagcgcaaa ccgtccaact
     1141 ggaagtcaaa atcgcgccga tgccggcaat gaaccacggt catcaccacg gcgaagcgca
     1201 tcagcactaa tctgctgaaa atgtttgaaa tgccgtctga aaaagcccgg gctttcagac
     1261 ggcattttta tgcccgcctt taaaatgtgt taaaatccgc ctttaaaaac cgccgtttcc
     1321 aaccatattc tacgcatgaa tacgacagca aacccttcca atatcatcgt cgggctctcc
     1381 ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc agcagggtta tcaagtgcgc
     1441 ggtgtgttca tgcagaactg ggaagacgac gacaacgacg aatattgcag catcaaacag
     1501 gattcgttcg atgccatcgc cgttgccgat attatcggca tcgatatcga catcgttaat
     1561 ttcgccgcgc aatataaaga caaagtcttt gcttattttc ttcaggaata cagtgcgggg
     1621 cgcacgccga atccggatgt gttgtgcaac gccgaaatca aattcaaatg ctttttggac
     1681 tacgccgtag ggcagggcgc ggataccatc gccaccggcc actatgcgcg caaagaagtt
     1741 cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa acaaagacca aagctatttc
     1801 ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt ttccgttggg cggtttggaa
     1861 aaacccgaag tgcgccgcct tgccgccgag ttcaaactgc cgaccgccgc taaaaaagac
     1921 agcacgggca tctgtttcat cggcgaacgc ccgtttcgcg agtttctgca gaaatacctg
     1981 ccgaccgaca atggtaaaat ggttacgccc gaagggaaaa ccgtcggcga acacgtcggg
     2041 ctgatgttct acacgctcgg acagcgcaaa ggtttgggca ttggcggcgc gggcgaaccg
     2101 tggtttgttg cggctaaaga tttgacgaaa aacgaactga ttgtcgtgca aggacacgac
     2161 catccgctgc tttatacccg cagccttgtg atgaacgatt tgagtttcac gctgcccgaa
     2221 cgcccgaagg caggacgcta tacctgcaaa acgcgttacc gtatggcgga cgcgccttgt
     2281 gagttgcgct atttggatga tgaaaccgcc gagctggtgt ttgacgaacc gcaatgggcg
     2341 gttacgccgg gtcagtccgc cgtgctgtac gacggcgata tctgtttggg cggcggtatc
     2401 atccaaacga ccgacaaacc cgtcatcatc acacgataaa ggtaatgccg tctgaaacgg
     2461 ttttcagacg gcattgttcc gctcaattcc actttaaaga ccgataccta tggaaaaaat
     2521 ctggttagac agctacgaga agggcgtcag tgccgaaatc gacatcacgc aatacaattc
     2581 cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc gaccaacctg cttttcaaaa
     2641 catgggtaag acgctgactt atgccgaaac cggcaaactg gcaacagatt ttgcttctta
     2701 tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg gcaataatgt tgcccaatgt
     2761 actgcaatat ccggttgccc ttttcggcat tttgcaggca ggtttggtag cggtgaacac
     2821 caatccgctc tataccccgc gcgagctgga gcatcagttg aaagacagcg gcgcgaccgc
     2881 catcatcgtt ttggaaaatt tcgccaacac gctggagctg gtgctgccgc gcacgcagat
     2941 caaacacgtc atcgtagcct ccgtcggcga aatgttcggg ctgcttaaag gttcgctgat
     3001 caatttcgtc atccggaaaa tcaagaaaat ggtgcccgaa taccgtattc gggaaaccgt
     3061 ttcctttcag acggcattga aagaaggggc gaagcacgtt ttccaacctg tcgcattaaa
     3121 ccgcgaagat accgcgctgt tgcaatacac gggcggcacg acaggtgttg ccaaaggggc
     3181 ggtgctgagt cacggcaaca tctgcgccaa tatgcttcag gcaaaagaat ggattaaaaa
     3241 ccagttgcgc gagggcaaag aaaccgttat cgccgccttg ccgctgtacc atatttttgc
     3301 cttaaccgtg aatctgatga tttttgccaa tgccggctcg aaaatcatcc tgattaccaa
     3361 cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa cagcgggtta atgtatttat
     3421 cggcgtgaac acgctgttta acgcgatggt taaccgtcct gattttgccg aagtcgattt
     3481 ttcagaattg cggctgactt tgggcggcgg tatggcgacc caaaaagccg ttgccgaaaa
     3541 atggaaaaaa atcaccggca cgcccatcgt cgaagcctac ggtctgaccg aagccagccc
     3601 cggcgtgtgc tgcaacccct taaacatcga atcatacagc ggcagcatcg gtttgcccgt
     3661 ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa gaagtccccg tcgggcagcc
     3721 gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc tactggaacc gccccgaaga
     3781 aaccgccaaa gccatagacg cgcgcggctt cttggaaacc ggcgatattg ccgtcatgga
     3841 cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac ctcgtcgttg tttccggatt
     3901 caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat cacggcaaag tcatggaagt
     3961 cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca ctcaaagtat tcgtcgtcaa
     4021 aaaagacccg tctttgacca aagaagaact tatcgctttt tgccgcaccg aattaaccgc
     4081 atataaagta ccgaaaaata tcgaattccg cgacgagttg cccaagtcca acgtcggcaa
     4141 aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa agaaaagatg ccgtctgaaa
     4201 acagccgtcc atcgttcaga cggcatccgt ccgtttgcca gaaccgcgcg cttcacgtta
     4261 aaatcacgca ttccaatacg ggtatttcat tatgaccaaa ttcattttcg tcaccggcgg
     4321 cgttgtctcc tcactgggta aaggtatcgc cgccgcttct attgccgcca tcctcgaatc
     4381 gcgcggcttg aacgttacca tgctcaagct cgatccttat atcaacgtcg atcccggcac
     4441 gatgagcccg ttccaacacg gcgaagtgtt cgtaaccgac gacggcgcgg aaaccgacct
     4501 cgacttggga cactacgaac gctttatcga ttccacgatg acccgccgca acagcttcag
     4561 cacgggacag gtgtacgaaa acgttatcgc caaagaacga cggggcgact acctcggcgg
     4621 tacggttcaa gtcattccgc acattaccga cgaaatcaaa cgacgcatcc acgaaggcgc
     4681 ggcaggttac gatgtggcga ttgtcgaaat tggcggcacg gtcggcgaca tcgaatcgct
     4741 gccgtttttg gaagccatcc gccagatgcg aagccagttg gggcgcaaca acaccttgtt
     4801 tgcccacttg agctacgtcc cctacatcgc cgccgcaggc gaaatcaaaa ccaagccgac
     4861 ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa cccgacatcc tgatttgccg
     4921 tatggacagg acaatgcctg cggacgaacg ccgcaaaatc gccttgttct gcaacgtgga
     4981 agaacgcgcg attgtcggca gctacgatgt ggacagcatc tacgaatgcc ccgaaatgct
     5041 gcacgaccaa ggcatcgaca acatcattac cgagcagttg cagcttaatg tgcagcaggc
     5101 ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa aacccgaaac acaccgtcaa
     5161 aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc tacaaatcgc tgatcgaagc
     5221 cttgaaacac gcaggcattc acaccgaaac cgacgtgcag attacctttg tcgacagcga
     5281 aaacatcgag aaaaacaagg gcgacgtttc catgcttaaa gacatggacg ccatcctcgt
     5341 tcccggcggt ttcggttcgc gcggtgtgga aggcaaaatc gccgccgtgc gctacgcccg
     5401 cgaaaacaac gtgccatact tgggcatctg cctcggtatg cagattgcgc tgatcgaata
     5461 cgcccgcgac gtggcaggct tgaaaggcgc gaattccacc gagttcgacc tcaaatgcgc
     5521 cgcgccagtc gttgccctga ttgacgaatg gcaaactgcc gacggcagcg tcgaaacccg
     5581 cgacgaatcc accgatttgg gcggcacgat gcgtttgggc gcgcaagaag tcgaattgaa
     5641 agcaggcagc ctcgccgcca aaatctacgg cagcggacat atccgcgaac gccaccgcca
     5701 ccgctacgaa gtcaacaaca actacgttcc tacactggaa caggcaggtt tggtcatcgg
     5761 cggtgtatct gccggacgcg aacgcttggt cgaaaccatc gaactgccga accatccttg
     5821 gttcttcgcc tgccagttcc accccgagtt tacctccaat ccgcgcaaag ggcatccttt
     5881 gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa gcctgataaa gcgttacttg
     5941 atgatcaaaa tgccgtctga aagcctgaat gaagactttc agacggcatt ttcgcaaatc
     6001 ggggattata aaagccatca atttaatttt tatggtttta tctaatattt accaactgaa
     6061 tattgttaag cggggcgtga aactcaagtt gagacctttg caaaattccc caaaatcccc
     6121 taaattccca ccaagacatt taggagattt ctcatgagca ccttcttcca gcaaaccgcc
     6181 caagccatga ttgccaaaca catcgaccgc ttcccgctat tgaagttgga ccgggtgatt
     6241 gattggcagc tgatcgaaca atacctgaac cgtcaaaaaa cccgttacct tagagaccac
     6301 cgcggccgtc c
//
LOCUS       DAWISC010000121         6267 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWISC010000121 DAWISC010000000
VERSION     DAWISC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.121"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..1184)
                     /locus_tag="WIW87_001567"
     CDS             complement(<1..1184)
                     /locus_tag="WIW87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ5174707.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WR"
     gene            complement(1189..1842)
                     /locus_tag="WIW87_001568"
     CDS             complement(1189..1842)
                     /locus_tag="WIW87_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ5174708.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            1993..2283
                     /gene="gatC"
                     /locus_tag="WIW87_001569"
     CDS             1993..2283
                     /gene="gatC"
                     /locus_tag="WIW87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ5174709.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            2346..3791
                     /gene="gatA"
                     /locus_tag="WIW87_001570"
     CDS             2346..3791
                     /gene="gatA"
                     /locus_tag="WIW87_001570"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ5174710.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            3788..4720
                     /locus_tag="WIW87_001571"
     CDS             3788..4720
                     /locus_tag="WIW87_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ5174711.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            4763..6193
                     /gene="gatB"
                     /locus_tag="WIW87_001572"
     CDS             4763..6193
                     /gene="gatB"
                     /locus_tag="WIW87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ5174712.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
BASE COUNT         1606 a         1899 c         1576 g         1186 t
ORIGIN      
        1 aaccgccaag cgcgttcggt atcggcggta taaatgcagg caccgagccc gaacggggaa
       61 tcattggcaa gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      121 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      181 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
      241 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
      301 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttagcgca ttcggcaaga
      361 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta
      421 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc acgctccaaa
      481 tcagcatcgg gcatcacgat aaaggcgttg ctaccgccga gctccaacac ggttttctta
      541 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat gcgttgaacc ggtaaacgcc
      601 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
      661 agaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
      721 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
      781 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
      841 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
      901 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
      961 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1021 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1081 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agcggggcgg
     1141 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1201 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     1261 catacccata atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     1321 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat
     1381 caacgcgatg tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc
     1441 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     1501 ggcggggcgc gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     1561 gttgccgttc atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa
     1621 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     1681 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     1741 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa
     1801 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     1861 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     1921 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     1981 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2041 ctgcacctga ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact
     2101 atggtcgaac agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac
     2161 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     2221 caggcgggtg ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa
     2281 taatccgaat atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     2341 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     2401 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     2461 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     2521 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     2581 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     2641 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     2701 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     2761 aaaacccatg gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg
     2821 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac
     2881 ccgcatcgca ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg
     2941 gtatggtcgc ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag
     3001 actgcgcgat tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg
     3061 agcgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg
     3121 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa
     3181 acaccattga tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa
     3241 ccaagctgtc catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt
     3301 cacgttacga cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa
     3361 tgtacggcaa aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg
     3421 gcacttatgt actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     3481 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     3541 cgaccgcacc cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact
     3601 tgagcgatat ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     3661 caggcttcag cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg
     3721 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     3781 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     3841 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     3901 agaaatttac gccttcgagc agtttgtcaa cgcatcccct atccgtcagg ggctgttcct
     3961 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4021 caaacgccgt ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac
     4081 agacatcctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4141 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4201 atctttgcgc gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac
     4261 acacctgctg acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg
     4321 ccgcataacc aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     4381 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     4441 caaactgcgc tggaaactta aaaagcgcgt caaaatgaat tacgacgcat tctggcagga
     4501 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     4561 agaccttgcc gacatcgaaa gcaaaaagcg ttcgatgtac cgcaagcgtt atgaaatgct
     4621 ggacaatatg gttgcagaga tgaaagacag tctgaaaaca gaagcacgcg gcatttcaga
     4681 cggcatccaa acggaaaaac cgccccgccg gacagcctga cgcgaagact atcgaattga
     4741 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     4801 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     4861 caacgcgcac gccagcgtag tggaatgcgc gctgccgggc gttttgcctg tgatgaaccg
     4921 tgaagtcgtt gaaaaagcca tcaaattggg tttggcttta gatgcgaaaa tcaatcagaa
     4981 aaacgtgttc gaccgcaaaa actacttcta tcccgactta ccaaaaggtt atcaaatcag
     5041 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5101 gaaaaccatc aacgtaaccc gtgcgcacat ggaagaagac gcaggcaagt ccgtgcatga
     5161 aggcttgaac ggcgcaaccg gtatcgacct gaaccgcgcc ggcacgccgc tgttggaagt
     5221 ggtatccgaa cctgaaatgc gttccgccgc cgaagccgtt gcctacgcca aggccttgca
     5281 cagcttggta acctggctgg acatttgcga cggcaatatg gcggaaggct cgttccgcgt
     5341 cgatgccaac gtatccgtgc gcccgaaagg tcaagaagag ttcggcacgc gccgcgagat
     5401 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cggaagcgca
     5461 aatcgagatt ttggaagacg gcggcaaagt acagcaggca accatgctgt ttgatcccga
     5521 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagatgcg cacgactacc gctacttccc
     5581 cgaccctgat ttgctgcccg ttatcatttc agacgcccaa atgcaaaaag ccaaagcaga
     5641 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggattacg gcgtgtccga
     5701 atacgacgcg cgcctgctga ccgcaagccg tgcgcaggct gcctattttg aagaagccgc
     5761 caaagaaagc ggacaaggca agctgactgc caactggatg aacggcgaac ttgccgccgc
     5821 gctgaacaaa gaaggcatgg aacttgccga cagcccgatt accgccccgc gcctcgccgc
     5881 gctggttggc aaaatcgccg acggcacatt aagcagcaag ttagcgaaaa aagcctttga
     5941 agccatgtgg gcagaacccg aagccaccat tgccgaaatc attgaaaaac acggtttgca
     6001 acagatgacc gacaccggcg agattgaagc catggtggac gaagtgctgg caaacaacgc
     6061 caaagccgtg gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6121 agtgatgaag gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6181 caaactggct taatccgtta tcacacaggt cgtctgaaag caaagttcca acgaaggtaa
     6241 aacaggaaat aagctttcag acggcct
//
LOCUS       DAWISC010000122         6259 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWISC010000122 DAWISC010000000
VERSION     DAWISC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.122"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(391..825)
                     /locus_tag="WIW87_001573"
     CDS             complement(391..825)
                     /locus_tag="WIW87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ5174713.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIW87_001574"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIW87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ5174714.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WIW87_001575"
     CDS             complement(1556..2398)
                     /locus_tag="WIW87_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ5174715.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WIW87_001576"
     CDS             2653..3291
                     /locus_tag="WIW87_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ5174716.1"
                     /translation="MFQNFDLGVFLLAVLPVLLSITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WIW87_001577"
     CDS             complement(3743..4543)
                     /locus_tag="WIW87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ5174717.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WIW87_001578"
     CDS             complement(4679..5182)
                     /locus_tag="WIW87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174718.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WIW87_001579"
     CDS             complement(5519..6160)
                     /locus_tag="WIW87_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ5174719.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT         1666 a         1570 c         1302 g         1721 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccaatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccagtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgca gcaggcgcgc
     2221 ctgcaccttc gggggcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctct ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatcta gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg cggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaggtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttacacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcaaaataa agcggcacgc ccgatttttc actcatcgtc atcaaataac
     5581 ccatcacctt ttggggccat tcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataag
//
LOCUS       DAWISC010000123         6164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWISC010000123 DAWISC010000000
VERSION     DAWISC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.123"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1048..3795)
                     /gene="tbpA"
                     /locus_tag="WIW87_001580"
     CDS             complement(1048..3795)
                     /gene="tbpA"
                     /locus_tag="WIW87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ5174720.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQHANRAYSSNTPPQNNGKKISPNGSETSPYWVTIGRGNVVTGQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPTDWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKEEAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTV
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3882..6029)
                     /gene="tbpB"
                     /locus_tag="WIW87_001581"
     CDS             complement(3882..6029)
                     /gene="tbpB"
                     /locus_tag="WIW87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpB"
                     /protein_id="HEZ5174721.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNWYPQAKEDEVKLDESDWEATGLPDEPKELPK
                     RQKSVIEKVETDSDNNIYSSPYLKPSNHQNGNTGNGINQPKNQAKDYENFKYVYSGWF
                     YKHAKREFNLKVEPKSAKNGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDTKKGQ
                     KFREIIQPSKSQGDKYSGFSGDDGEEYSNKNKSTLTDGQEGYGFTSNLEVDFHNKKLT
                     GKLIRNNANTDNNQATTTQYYSLEAQVTGNRFNGKATATDKPQNSETKEHPFVSDSSS
                     LSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASNGAAG
                     TSSENGKLTTVLDAVELKLGDKEVQKLDNFSNAAQLVVDGIMIPLLPEASESGNNQAN
                     QGTNGGTAFTRKFDHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSN
                     LNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPSEQNIVY
                     RGSWYGYIANDKSTSWSGNASDREGGNRADFTVNFGTKKINGTLTAENRQEATFTIVG
                     DIKDNGFEGTAKTADSGFDLDQSNTTRTPKAYITDAKVKGGFYGPKAEELGGWFAYPG
                     DKQTEKATVTSGDGNSASSATVVFGAKRQKPVQ"
BASE COUNT         1292 a         1552 c         1553 g         1767 t
ORIGIN      
        1 ctataccgtc gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa
       61 aaaccttccg ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt
      121 aaataaattc ttgcagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc
      181 gaaagcggta atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat
      241 cccgtcattc ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc
      301 attcccacgg aagtgggaat ctaggacgta aaatctcaag aaaccgtttt atccgataag
      361 tttccgcacc gacagacctg gattcccgcc tgcgcgggaa tgacgaaatt tcggcgagcc
      421 gtagggtggg ctgtagggtg ggcttcagcc caccaatttc accgcatcaa aggtttttgg
      481 gaatacggcg gtttcggttt tcggtgggaa tgggcggaat ggataacatt gacggagttg
      541 ggggaatagg tggaaacggt gggattggtg ggctgaagcc caccctacag ccagccctat
      601 gtccacgctg cacatcctac gcccgccttg cgtttcttat aagcagcatt ttacgcaata
      661 ctttgcgtcc gctgaaaaaa tttcaaacgg tcttttttat caaatgcgaa aaatataacg
      721 tcattcccgc gcaggcggga atctagtctg tcggtgcgga aacttatcgg ataaaacggt
      781 ttctttagat tttacgccct agatcccgtc tgctcgggaa taacgggatt tgaggtttct
      841 gtttttgatt ttctgttttc gcgggaataa cggtttagaa gttacccgaa acctcaaaaa
      901 aaccgaaacc gaacggactg gattcccgcc tgcgcgggaa tgacgggttt cgagattacg
      961 gtgtatcggg gatgatggaa aatggcgggg attgtgtaaa aaatgccgtc tgaagccttt
     1021 cagacggcat ttgcggcgtt tggacgttta gaacttcatt tccaagctaa atgtgtagtt
     1081 gcgaccgggg gcggcatatc ggttgtaaac gccgacattt ttgtgttggt tgactgcgcc
     1141 gccggcagtt tgccgcacat tttcccaagt aacatagcgg tagttgagga ggttgtacac
     1201 gcccgcacgg agggtaaagt gttttttaac cgtgtaataa ccggacacgt ccacaatata
     1261 ccaagggcgg gtacggcgcg cggtggcttt tgtattgcgg ctgttgccgt tgagcaaagc
     1321 ccggctgccc aacaactctg tgatttcctt ggctttggaa taagtcagca taccgttcac
     1381 accccatttg ccttccggtt ggtcatagcc caagccgacg acatagcgcg agggttggat
     1441 ggcatcaaac agatgtgatt gaatatcggt gcggtctgcg cgttttttga tgtcgcggac
     1501 acggacacga ttataggcaa atgtagaata ccaaccttcg ggcaatttat cccatacgcc
     1561 gttccaatcg attttgccca aaatattgat gccggtaatc cgcgcgcttt gggcattgag
     1621 gtaagccggg tcgcctttgg cttcttcttt gccgtcttta atttgcgctt cataaccccg
     1681 gacaatcaaa tcgcggtagg cattgttgaa ccaacttgcc tccaagttgc cgaaatcgcc
     1741 tttaaacacg atgccggctt ctttgttgaa cgatttttcc ggatcgattt tgaccgcctt
     1801 gctttgaaca cccgcccgcc agccgtacat ttccgcaaac gagggcaggc ggaagccggt
     1861 tgaggtgcgg taagtcaaat ccagccagtc ggtaggtttg aggacgatgc cggcgttcca
     1921 ggacaaggtg cggtgcgtgc cggtggaaac gctgccgtcg tccgaatgcg tgctgcggta
     1981 gtcgtagcgc aagcccgcgc cgacatccgc ccacctgccc aaacggacat tgtcccgaac
     2041 tgccgcgtaa tagcttttac cgttgatgct gcgcggcgtg cagtccgtat aagtattgtt
     2101 gcccaagcgg cagatttgcc ccgtaacgac atttcccctg cctatggtga cccaataggg
     2161 gctggtttca ctgccgttgg ggctgatttt tttgccgttg ttttgagggg gcgtgttcga
     2221 cgaataggcg cggttggcat gttgataata ataatcctga tggcggagat tagagccaaa
     2281 gcggtcaaac ccgagattca cgctcaggtt gtggcggatt ttggcggtat cgaaggattt
     2341 tttgaatgcc gcctgcaaga gcctgtggct ttccccgtaa atcacgcgat cggatttgta
     2401 ataggaaaac ggcttgtcgg cactcgggcg gcaatatttg tccgaaccgt cggcagaaca
     2461 gtgcgtctgc tgaaaatgat tatccaaacc gataccctgc cggtcgtaag agaggcgggc
     2521 ataatccgcc caagtgtctt tatcggcatt ggtatagaca tattccaaac cgtagcggct
     2581 tttggtgtgc gtctcgtcgt aaaacacgcc cgtaccgtat tccgcgccca ccagcgcacc
     2641 gttttcgccg ttggtaaaca gtccgccgta tttgtggttg cccgcgtatt tgccgttgcc
     2701 gggcaaagaa cccgcctgtt ttgaatttgc atcaaaaacc gccttggtca ggaatgccgg
     2761 aaccgtcata tcgcgcgtgt cgaaagtttg ttgcgtgtgt tcgagtatgc cgccgatgta
     2821 gtgccgctta ttttcaaaac gaaaacccgg gcggaacagc cacgatcggc tttcgtatga
     2881 aagcggatcg gcgaggaagc ggtttgggcc cgtgtagtct cgggtggaaa ccgtttgacg
     2941 ttcgtctttg ccgacaacat cttttttcgg attcgcttta cacgtttcat aactcccgtt
     3001 tttgcattct tctttaacga tgaaataggc gtaattgctg ctgtcttcaa ccggcaccag
     3061 cctgttaaag ctctgaacgc cgcgtccggc atcttcgtgg gcgcggattt cccccgcgcg
     3121 ccggccggtg tggatcagca aagcctccgc accgccgatg cgccccgcca gcgcgatgga
     3181 ttgggtaagc ccccggtttt tgccggaata ggcggtttta ctctgaatgc cccactgcct
     3241 gccttccccg ataacatcgt cggcggtttt ggtttgaaat gcgaccgagc ccgccaatgc
     3301 gccgctgcct tgttcgaccg agtttgagcc tttgctgatt tcgacagcct tgacgttttc
     3361 atactcgatt tcattgattg cgccgctgct gcccgccgtc ctcgtcccgc ccaatgccgc
     3421 ctgcgcggtg taggactgta tttgcgccaa gccgtccacc gtcaaggaaa cgcggttttt
     3481 gtccataccg cgtatcgaat aacccgagct tgcgccgcgc ccctgttcga ccacggcgat
     3541 gccggggtcg tagcgagtca ggtcgcggat atcgagtacc tgttccttgc tgagggtgtc
     3601 ggcggttttg accaatttgc ccaaaccggt tacttcgtta tcgcggcggg ttttctgttt
     3661 tttggctttt acctgtatgg tatccaactg tttttcctgt gcttgtccgg cttgcacatt
     3721 ttctgcataa acgggcagcg cggtcatcag cgacaggcat aaaatattta atcggaacaa
     3781 atgttgctgt tgcatagtgt ttccctaatc ttcgctttca gacggtatcg gaaggagcga
     3841 tgccgtctga ggccttattc ttgattgttc ggcagccgtg cttattgcac aggcttttgg
     3901 cgtttcgcac cgaatacgac agttgcactg cttgctgaat ttccatcgcc ggatgtaacc
     3961 gttgcctttt ccgtttgttt atcgcccgga taggcaaacc atccgcccaa ctcttcggct
     4021 ttaggcccgt aaaaaccgcc cttcaccttg gcatctgtga tatatgcctt aggcgtgcgg
     4081 gtggtattgc tttgatcgag atcaaaacct gagtcagcag ttttcgccgt accttcaaag
     4141 ccgttgtcct taatatcgcc cacaatggta aaggttgcct cctgcctgtt ttcagcggtt
     4201 aacgttccgt taattttttt cgtaccaaaa ttcacggtaa agtccgccct gttgccgccc
     4261 tctctatctg aagcattgcc gctccagctt gtgcttttgt cgttggcaat atatccgtac
     4321 caagaccccc gataaacgat gttttgctcg cttggaatct ctttttcatc ggtgcgctcg
     4381 ccttggagga acatactttg ttcaacttgt tccgttttag catcagcttg actactgctt
     4441 tctcctgcct gcatcgcgga cttgctgttt ttgcgcgtca acattccgta tttcagataa
     4501 ttgaggttgg aacagcagac ttcgacttca taggtttttg ttttgccatt ggtatcacct
     4561 gccgtatttg aagcggtttg cgccccattc gtctgcgtac ctgcttgggc gtctttttta
     4621 tcactttccg gcgtgtggtc aaatttgcgg gtaaaggctg ttccgccatt tgtaccttga
     4681 ttggcttgat tgttcccact ttcggaagcc tcgggcaaga gcggaatcat aatgccgtcg
     4741 acaaccagtt gggcggcgtt gctgaagttg tcgagctttt ggacttcctt atcgcccaat
     4801 ttcagctcga ccgcatccaa aaccgtggtc agcttaccgt tttcagacga cgtgcctgcc
     4861 gcaccgtttg atgctgccgc atctgtgccg cctgaagccg ccgcagtatt gccatttgcg
     4921 ggtttgtctt tggttttcgc gctgccgaca acggcaactt tttgatcgtc gctcaaaaag
     4981 cggaaaccca attcctcacc ctgcgggccg aaaaagccgc cgctcaaaga agacgaatcg
     5041 gaaacaaagg gatgttcctt ggtttcgctg ttttggggtt tgtcggttgc cgttgccttg
     5101 ccgttgaagc ggttgcctgt tacttgagcc tcaaggctgt agtattgcgt ggtggtggct
     5161 tggttgttat cggtattcgc attgttgcgt atcagtttgc ccgtcaattt tttattatgg
     5221 aaatccactt ctaaatttga ggtaaaacca taaccctctt gaccatctgt cagcgtggat
     5281 ttgtttttgt tggaatattc ttcgccgtca tcgcccgaaa atccgctata cttgtcgcct
     5341 tgactttttg aaggttggat aatttcacga aatttttgac ccttttttgt atcggtcgca
     5401 aaatgccaca cacctttata ggtaattttt ccagaagcgg gaagttgtcg ggaaggttct
     5461 ttaccgtgat agaagatata accgtcgtcg ccgttttttg cacttttagg ttccaccttt
     5521 aagttaaact ctcgtttggc gtgtttgtaa aaccagccgg aataaacata tttaaaattt
     5581 tcgtaatctt ttgcctgatt tttaggttgg tttataccgt tgccagtgtt gccgttttga
     5641 tggtttgatg gtttgagata gggggaagaa taaatattgt tgtcgctgtc tgtttctact
     5701 ttttcgataa ccgatttttg ccgtttaggg agttccttag gttcgtccgg caatcctgtc
     5761 gcctcccaat cactctcgtc cagtttaacc tcgtcttctt ttgcctgcgg ataccaattc
     5821 ctccgtttca acctcattgc aaaaccgtat ccgccttggt ctttttgggc ttgcggtttt
     5881 tcggaagaaa catcttgata ttttggcgcg ggacgcgggg cttcggtatc gacagaatca
     5941 agatcgaaac tgccgcctcc gcccaaacaa gcactcaaca aaaacacagg cagcaccata
     6001 gcagcctgat ttaccaatgg attgttcata taaacccaat tcaattaaag aatgataagg
     6061 attattattt tatttatttt taacaaattt gcaaatactt ttttattttt ttagggaata
     6121 caccaaaatc ccgtcatttc cgcgcaggcg ggaatccgaa cgcg
//
LOCUS       DAWISC010000124         6161 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWISC010000124 DAWISC010000000
VERSION     DAWISC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.124"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(174..1307)
                     /locus_tag="WIW87_001582"
     CDS             complement(174..1307)
                     /locus_tag="WIW87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174722.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGKGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFRKESNRIASDSRNSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(1427..1921)
                     /locus_tag="WIW87_001583"
     CDS             complement(1427..1921)
                     /locus_tag="WIW87_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174723.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     AFALEDWEISGKPKKWEKWTEKYQTEALQSVKNLFSEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERLGRPLDWKKWKDQYQSEAFELVKKLNR
                     SKQE"
     gene            complement(2070..2405)
                     /locus_tag="WIW87_001584"
     CDS             complement(2070..2405)
                     /locus_tag="WIW87_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174724.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            complement(2417..2776)
                     /locus_tag="WIW87_001585"
     CDS             complement(2417..2776)
                     /locus_tag="WIW87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174725.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNGNLIQTTN"
     gene            complement(2997..3452)
                     /locus_tag="WIW87_001586"
     CDS             complement(2997..3452)
                     /locus_tag="WIW87_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174726.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITEYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKVVTIIAGGKDNYSTEPYYDE"
     gene            complement(3433..4062)
                     /locus_tag="WIW87_001587"
     CDS             complement(3433..4062)
                     /locus_tag="WIW87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ5174727.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGKTGYKPVIARYGNPYQ
                     ETVYIEVSDGIGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLFAESGKTQTVRNIIV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPKRTGSSKNGKHGDGGRSQIS
                     AESRIAELKNKIIPGMSKNERLKIKQKIRNIAKNANRKAEGEEHGRRGR"
     gene            complement(4207..4437)
                     /locus_tag="WIW87_001588"
     CDS             complement(4207..4437)
                     /locus_tag="WIW87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174728.1"
                     /translation="MEKITQQYAYSELLRLFNQNASDEKIANLAFDFLYAWSKDNSPE
                     SRNIIYDLALIGEPGMELTRNDIKELIDSLVE"
     gene            complement(4425..5699)
                     /locus_tag="WIW87_001589"
     CDS             complement(4425..5699)
                     /locus_tag="WIW87_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713332.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ5174729.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     YEEHAPFDNHAGHSASKEKGNVDEGFTVYHLNWEGHEHHPADAYDGPKGINYPKPTGA
                     RDEYTYHVNGIARSIKLNPTDTRSIRQRISDNYSNLGSNLSDRAYEANRKMFEHNAKL
                     NRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLT
                     KAAKAAKAAKLAKAAKPGKAKVSGDFAVATQAKYKQYGDAHSANAQSALAKKLSGLED
                     AQNSASRTKTLPDGRIRYYEAERFARTEGRTRGNSFVTEYNPKTGSTRQWTENYDHSG
                     NVTMVHPKSINGQSVTSQHYPPTKQELDSWKK"
     gene            complement(5703..>6161)
                     /gene="mafA"
                     /locus_tag="WIW87_001590"
     CDS             complement(5703..>6161)
                     /gene="mafA"
                     /locus_tag="WIW87_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ5174730.1"
                     /translation="PRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRS
                     RTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGK
                     TVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
BASE COUNT         1477 a         1418 c         1354 g         1912 t
ORIGIN      
        1 gtggaaaacc tatgggatga tacggtcggt aaaaagaccc gtttaatgtg tatttccgtt
       61 ttttggattg tggttttcaa tttgtagcga atcggattcg gcatatacgg cattgcaaaa
      121 agcgtttgac tctccaatgc cgtctgaaaa ccggtttcag acggcatttg cgttcagtga
      181 gaaaggtcgc gcctgccgcc cgaacgtctc gccgcagcct ctgcataacg gcgcacctct
      241 ttttccaaat tttccaagtt caaaggaaaa tcaggcagtc tgtctccctg tttctcttcg
      301 cggacaatcc gcccgccatc caaataccac gtctgttgcg catgataggt ctgcatatcc
      361 gccgttacgc catccgcttt caatgctacc gtcgaagatt gtgcaataaa aagatttccg
      421 tttttcaaat aatattcgaa actctggcgt ttttttccat tgtcgaaact ccaatagact
      481 ttttgcggca gaccgtccgc atcatagccg accacaagac tgttcgcctt catccctcgg
      541 ggcatcaatt cccgcatatt ctgataaaac acagaattgc gcgagtccga cgcaattcgg
      601 ttgctctctt tgcggaagtc ccaaaccttc tgctcgtcat tcgcgacatc ccggtatttc
      661 gccaaatata cctgggccat ctgataacac ccgaggcaat gctcataaac atcttccccg
      721 attttcccgc gccccgccgc atcaaatacc gaaccgtctg gttgccaaac aacccgatat
      781 tctcctgtcg tttcataatt ttccccgtga accgttccgc cgtacacatt tacagaaaac
      841 ggacgatcgt tccgatacag atattcggca ttaacaaacg cttccggcga gcgttgcaaa
      901 agcgaaaccg caaccaaacc gccctcgccg atatagtaat ccagccagac ctcttcccca
      961 tgttcctgct ccgttacgtg aaaccatttc gccttttctt tcaaacgact gagccggata
     1021 gcgagcgcaa gataatcctt ctccgactgc aacggaccgt catccacagt tccggcaaga
     1081 ctttcctccg tcctcatcga ttccttcacg atgacaaccg ccctgtcggc atttcggaac
     1141 aggcgggcaa gtttcgccac aaaagcattc ggattttcag gtacttcagt tgccgtatcg
     1201 ctcaaaaacc aacgcggatt aatctcatag gcaataccct tccccagcca aaaggcaaat
     1261 acaagtgcaa aaaatgacaa caataccggt ttgaattttt taaacatatt tatttttcgt
     1321 ttaacaggat atatcgatta tatcaggcga gctttgattg tttgattttt aatctaatct
     1381 acggttgatg ccgtacttta aaaaccacta tgcaggctgc ggcttgttac tcttgtttgc
     1441 ttcggttcaa ttttttgact aattcaaatg cttcagattg atattgatct ttccattttt
     1501 tccaatctaa cggccttccc aagcgttccc aatcttctaa aaccgacatt ttgaacattt
     1561 ccatttccca ccaatattcg gcatatgaac gtgattgatt gggaatgtcg gcttctgccc
     1621 gatcggcaaa atgcaaaaaa tgagaaatat cgcaaactat attattttca ggaacttctt
     1681 cggaaaatag attcttgact gactgtaacg cttcggtctg atatttttct gtccattttt
     1741 cccatttttt aggctttccc gatatttccc aatcttccaa tgcaaaggca ttcaagattt
     1801 ccaattccca ccagcagtcg taataacgag aggattggtt aggaatctta ttttccgctt
     1861 cggaaacaaa ttgggtaaaa ctaaaaatat ccttaacgat tttttcaaat ctattttcca
     1921 taggttgccg ccccctccaa attgcgtgcc aacaactccg gcaaaatcaa gtcggcaagc
     1981 cccggcatcc tgtgccaatc ggagaaaatg ccgtctgaaa gccgaatacc gcttcagacg
     2041 gcatttgcca gatttgacct tgccggtgct tacggacgct ttttaaatat aaagaaggaa
     2101 atatcgtcta acgcatcttc ggcaaaacct tgaaaatccg gattaccttg ttttgagcgc
     2161 aataaagcaa tcactttatc gcaatcgccg atattttcat gcatcctggc aagatgtccg
     2221 aaacaggtta atgacaatct ggcaatatcc aagtctttat gcttcgacaa ctccgtcaat
     2281 ttctcagaaa tccaagggat ttcatcctcc tccgagctga aggtcatacc gagcataccg
     2341 tcaataacac tctgtttatt ctcagaataa atcatctttt ctacttgttc cttatcagcc
     2401 atcatttttt gtccatttaa ttagtcgttt gtattaaatt gccattattt attttccaat
     2461 ttactttata actatcttca taataatcta attcaaaaaa acctgatatt tcaatatcca
     2521 attccattat tgttttaata catttttcaa aataaataat gaaataagat tttacgcatg
     2581 caccaaaaaa aatatagctg ctccaattaa aactatttgt cgggaaaacc cacccgcttt
     2641 tatatatttt tgcagattct ttttcttcga tattgaaggg gcaattattc caaaaattat
     2701 taatgttttc ttggatgatt tttatatcat cgtcagagca ttcaatccat cctttgatgg
     2761 aaacatatga tgccatgttt aatctcctaa acctgtttta acaatgccgc cttttgattc
     2821 aatatatgac ttaacttgtg aatgaacacc gtgcctgttc cgtacagcaa gcacaggttg
     2881 gattaagatt cgacaatctg tttggattga cagaaaggtc gtctgaaaaa tcgattacgg
     2941 tttcagacga ccttttgttg ttgagttttc aacccaacct atgcctgcca ttaatgttat
     3001 tcatcgtagt aaggttctgt tgaataattg tctttgcctc cggcaatgat agtaacaact
     3061 ttcccttttg cttcccaagc ttgtactcct atttcatcaa actcatagac atatgtcgga
     3121 taagattcat ttgataaata atatttatca acaccgtatg atttagggtg atggaaaagc
     3181 tgtttaaaat cttcaaaatt cagacctatt atattaacgc ccataaaata tagctcctga
     3241 taacaaaata tagaaataat tttgtttttt tcgacggaaa tgagtaaatt tgagtcggga
     3301 gattcataat attctgttct aaactcatca ggaggttcat acataaaagt ttccagtatg
     3361 tttttgtact ctgttatatc cgcaccaaaa cggaatattc ctacagaagt aaaaggtaaa
     3421 aattcgggag ttttaacgac cgcgtcgacc atgctcttct ccttctgctt ttcgattggc
     3481 attttttgca atatttctga ttttttgctt aatctttaag cgttcatttt tggacattcc
     3541 gggaataatt ttatttttta attcagcaat tcttgattcc gctgatattt gacttcgacc
     3601 gccatctcca tgttttccat tcttggagct tcctgttctt ttaggcggac acgcattatg
     3661 aacccaaacc ccttccgttt ccgcctgatt gcccttgacg aagtaggtat gccaatcggc
     3721 aacggtcaga ttgtaggctt tgagcggttt tggtttgaca atgatgttgc ggacagtttg
     3781 ggttttgccg ctttcggcaa acagcctgct tcccgctttc aaatcttccg ctttaatcca
     3841 tttgccgccc gaataaaacg gatggatgcg gttggaaatc agggtttggt tgttgccgat
     3901 gccgtctgaa acttcaatgt aaacggtttc ttgatacgga ttgccgtatc gggcgataac
     3961 gggtttgtat cccgtttttc cgcttgcctc gtccttggaa aggacgctct cgccggctcg
     4021 gatacgggca atagctttgt agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     4081 gcaggtgtag gatttttgaa gaacttagtt ctcaatatcc tgtttcaaat gaaggaaagg
     4141 tcgtctggaa attaaaagca gtttcagacg accttttgat tgtgggattt tcagcctaac
     4201 ctatacttac tcaactaaac tgtcaatcaa ttcttttatg tcattacggg tcagttccat
     4261 acccggttca ccgataagtg ctaaatcata gattatatta cgcgattctg gagaattatc
     4321 ttttgaccaa gcatacagaa aatcaaaagc aagattcgca attttttcat ccgaagcgtt
     4381 ttgattgaac aatctgagca gttcagaata tgcgtattgt tgagttattt tttccatgaa
     4441 tctaattcct gttttgttgg tggataatgc tgagaggtta cactttgtcc gttaattgat
     4501 ttaggatgaa ccatagttac gttcccgcta tggtcataat tttccgtcca ttgtcgggta
     4561 ctgcctgttt taggattata ttctgtaaca aagctattac ctctcgttct tccctcggtt
     4621 ctagcaaatc gctcagcttc ataatacctg attctcccat caggtaaagt ttttgtccta
     4681 gacgcactat tttgagcatc ttccaaacct gacaatttct tagctaatgc cgattgggca
     4741 ttggcagaat gcgcatcccc atattgcttg tatttggctt gtgtggcaac ggcaaaatcc
     4801 ccgctgacct tcgccttccc cggttttgcc gcctttgcca acttcgcggc tttggctgct
     4861 tttgccgcct ttgtcaggtt tttgactttg gcaaacggca ggacgttgac cagggcttcg
     4921 acggtttcgg cggcattggg gttttcctgc atccactggt caacggcttc gcccgcgctg
     4981 ataaaggggt taagcgcgcc ggcggcgacg ccgttgataa actccatgct gttgccccag
     5041 cggttgagct tggcattgtg ctcgaacatt tttctgttgg cttcataggc gcggtcggag
     5101 agattgctgc cgaggttgct gtaattgtcg gatatgcgtt gccggatgct gcgggtgtcg
     5161 gttggattga gtttgatgct gcgggctatg ccattgacgt gataggtgta ttcgtctcgt
     5221 gcgcccgtag gtttggggta attgatgcct ttgggcccgt cgtaggcatc ggcaggatgg
     5281 tgttcgtgtc cttcccagtt gaggtggtat acggtaaagc cttcgtcaac gttgcctttt
     5341 tctttgcttg cgctgtggcc ggcgtggttg tcgaaggggg cgtgttcttc gtatccgtgt
     5401 ccggaaaagc ggacggtgta accaagattg ccttggattg ccgcctgttg gatgagcagg
     5461 ttgcccatct ggtgggtata gtcttggatg acgttgattt gaccggtgcg gtcggaaacg
     5521 ctgccgcgcg ggtcgccgaa gaggtggtat ttgccgccgg gttcgtagtg ctgccgttgg
     5581 gtgttatcgg taatgaacgg gtcttgcgcc aagtccgccg cgagggcggg ctgtatgagt
     5641 gcggccgccg ctacggcgca ggcggcaagg aggtttgtta gtctgcgcag cggtttcacg
     5701 gtttatcctc ctttgcggcg gcggatgact tcgttgccga catcggggtt tttaccgttg
     5761 ttttgtttga agtcgggacg gttttgggcg gtcgtgtcgc cgtagggggt gatgtcggag
     5821 aaatcgacca tcaggcggtc tgaggctttg acggttttgc cgacgctgta aggtcccatc
     5881 cagagggcgt attgttcttg gtattgggat tcgtaggcgg cggttttagg ggcaatcagc
     5941 agtttccggc tgtcgcggtc gacggcgaaa tattcgagct tggtttgggc tttaagggtt
     6001 tcggcgttgt agaggtgtag ttcggtacgg ctgcggacgg tgccgaatac gtcgacggtt
     6061 acgaatacgt cggtgtcggc gtattcgggc ggtacgactt cgatgccgcg caggtagaag
     6121 acggtttgaa tgaggttagt caggaaggaa acgtcgcggg g
//
LOCUS       DAWISC010000125         6138 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWISC010000125 DAWISC010000000
VERSION     DAWISC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.125"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WIW87_001591"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WIW87_001591"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ5174731.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WIW87_001592"
     CDS             complement(1612..2487)
                     /locus_tag="WIW87_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5174732.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2489..3211)
                     /locus_tag="WIW87_001593"
     CDS             complement(2489..3211)
                     /locus_tag="WIW87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ5174733.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WIW87_001594"
     CDS             complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WIW87_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ5174734.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3788..4150
                     /locus_tag="WIW87_001595"
     CDS             3788..4150
                     /locus_tag="WIW87_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ5174735.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4185..5048
                     /locus_tag="WIW87_001596"
     CDS             4185..5048
                     /locus_tag="WIW87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ5174736.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5141..6100
                     /gene="ttcA"
                     /locus_tag="WIW87_001597"
     CDS             5141..6100
                     /gene="ttcA"
                     /locus_tag="WIW87_001597"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ5174737.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT         1565 a         1779 c         1482 g         1312 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtat attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgcagccgc
      361 cattgaaaaa gcctgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccctc
      421 cgacgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggctatga gaaaggccgc tacgacatcg tcaatccgat
      541 ggaccatgtc aacgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      601 cgtgtattac agtatcggcg aattgctcga caaactggtc gttctgaaaa acgcctttgc
      661 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccaatg actacaggac aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcacgc gccaactgct tttagaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgatacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg cagcaatcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatatgcg agcgttacgt tttcaactcc atcgggctgg ttacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtacc ttgacttccc cccaataaaa caggcaggcg atagaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaag aacaatgcgc ccgaaagcgt
     1741 acccaaaaca atcacgccga tattcatacc ggcaaaaacc aaggtcggat tatccttcat
     1801 catttggtgc gcggtgatgt aggttacgat attcataaaa ttcaaaccgc ccaagaatat
     1861 gccgccaacc acactctcaa cacgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcacaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 tgccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3241 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc cgggacggat gccgataatg
     3301 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3361 taaatatcca actttgtccg gatgccgaac ggcgttgtgc cgccgaccag atagcccgtc
     3421 cacttgtttg cctgtacggg cgtggcgggt tcaatgtgtt tcgcacccaa atgccgcgcc
     3481 agattgcggg ttgaaatctg cttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3541 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa tagtcgggca
     3601 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3661 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3721 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3781 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3841 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3901 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3961 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     4021 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagcacg
     4081 gctcgcccgt ccggagtggc gcgtcgaaat caaaataacc gccgtgaaga gagacgcggc
     4141 aactgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4201 cctttttcgg cgtggtgcgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4261 tttccaacga catcaaccac cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4321 ccaaaggccg cgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4381 ttatggcgca agacctgctt gaagcaacgg tcaaacgttt gcggatgttt gtgttacgcg
     4441 ccaaagccgt ttttgaaatc cttgaagatt acgccgtcgg cgcggaatta gaagcatccg
     4501 ccgaaccgct tgccgcgcaa gaacccagcc tcgcctttac cgccgaatgc ggttcagacg
     4561 gcatctgttc cgtcgtcctg ccacatcggg gcattttgca catcgccccc aaaaacgcct
     4621 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgtagcggtt
     4681 atccgtggat atgcgcggct accaaagaaa ccgccgtcgc ccaaatgctc aaccagcaca
     4741 tcatcggcgg cgtacacttc aaaaaaggct gctatccggg acaggaaatc attgcccgtg
     4801 cccagtatcg ggggcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4861 aagcaggaac cctgctgacg gcggacggcg aagaggccgg catcgtactc gacagcgttc
     4921 aagattcgga aaactttacc gcccttaccg tgattaaatt ctccgccgca caaaaaacac
     4981 tgaccgtccc aaacggcggt atcttcaaag cggttcatct cttctttaaa accgaaaatg
     5041 cagaataagc cgccgatgcc gtctgaagcc cttcagacgg cattgccgcc ccgccaatcg
     5101 gttacaatgc cgcctttcta attttcaaac ccaaaccgcc atgtccaaaa aaaccaaaca
     5161 agaactcgaa aacaacaaac tcagcaaacg cctgcgccac gccgtcggcg acgcgattaa
     5221 cgatttcaat atgatcgagc cggacgacaa aatcatggtc tgcctctccg gcggcaaaga
     5281 cagctatgcc ctgttagaca tcctgcgcca gctccaagcc agcgcgccga ttgatttcca
     5341 actggttgcc gtcaatctcg accaaaagca gccgggcttc cccgaagaag tattgccgac
     5401 ctatctcgaa agcatcggcg ttccctacaa aatcgttgaa gaagacacct actccaccgt
     5461 caaacgcgta ttagacgaag gcaaaacgac ttgttcgctg tgcagccgcc tgcgccgcgg
     5521 catcctctac cgcactgcaa aagaattggg ctgcaccaaa atcgccttgg gacaccaccg
     5581 cgacgacatc ctcgccacct tgtttttaaa tatgttttac ggcggcaaac tcaaagccat
     5641 gccgcccaag ctggtgagcg acaacggcga acacatcgtc atccgcccgc tggcgtatgt
     5701 gaaagaaaaa gacttaatca aatacgccga attaaaacaa ttccccatca tcccgtgcaa
     5761 cctctgcggc tcgcaaccca acctgcaacg ccaagtcatc ggcgatatgc tgcgcgattg
     5821 ggacaaacgc ttccccggcc gcatcgaatc gatgttctcc gccctgcaaa acgtcgtccc
     5881 ttcccacctt gccgataccg aactcttcga ctttgtcgga ttggaacgcg gtcaaagcct
     5941 gaaacacggc ggcgatttgg cgtttgacag cgaaaaaatg cccgaacgtt tttcagacgg
     6001 cagcgaagaa gacgagagcg aaatcaaaat cgagccgcaa aaagccgaac gcaaagtcat
     6061 caatattctg gcgaacaagc cgaaaacctg cggctcatag acctccgccg tcaaaaatgc
     6121 cgtctgaagc ccgtttgg
//
LOCUS       DAWISC010000126         6128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWISC010000126 DAWISC010000000
VERSION     DAWISC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.126"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(55..1566)
                     /gene="ubiB"
                     /locus_tag="WIW87_001598"
     CDS             complement(55..1566)
                     /gene="ubiB"
                     /locus_tag="WIW87_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ5174738.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(1601..2065)
                     /locus_tag="WIW87_001599"
     CDS             complement(1601..2065)
                     /locus_tag="WIW87_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ5174739.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(2062..2208)
                     /locus_tag="WIW87_001600"
     CDS             complement(2062..2208)
                     /locus_tag="WIW87_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174740.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(2225..2563)
                     /gene="erpA"
                     /locus_tag="WIW87_001601"
     CDS             complement(2225..2563)
                     /gene="erpA"
                     /locus_tag="WIW87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ5174741.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(2695..3381)
                     /locus_tag="WIW87_001602"
     CDS             complement(2695..3381)
                     /locus_tag="WIW87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5174742.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3569..3841
                     /locus_tag="WIW87_001603"
     CDS             3569..3841
                     /locus_tag="WIW87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174743.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            4049..5977
                     /gene="dnaK"
                     /locus_tag="WIW87_001604"
     CDS             4049..5977
                     /gene="dnaK"
                     /locus_tag="WIW87_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ5174744.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1757 a         1649 c         1344 g         1378 t
ORIGIN      
        1 ctataaccga tttccttata aacgggcgga gtcgggcgat gattcggaca aagcctattt
       61 caaaagcaaa atcagcagca aaacaaccgc aatcacagcc agccacaggc tttgccgctg
      121 ctgcactttg accaaatgga tataggcatc acgcatttcc tgctggcggt tttcatcaat
      181 caacgcactg attttgcgcg gcaatgaagg gatgatttgc gcccagtcgg gggcttcgtt
      241 tttgaggttg cgccaaaggg ctttagggcc gacctgcccg ttcatccatt tcaccaaaaa
      301 cggtttggcg gttttccaca agtccaaatc gggatcaagc tgccgtccca agccttcgat
      361 gttgagcagc gttttttgca gcaataccag ctgcggctgg atttcgacat tgaagcggcg
      421 gctgacttca aacaggcgca tcagcaccaa gccgaaggaa atctgcgaaa tcggtttgtt
      481 gaacactggt tcgcacacgg cgcggacagc cgcttccaac tcttccgcgc gcgtgtcggc
      541 gggcacccag cccgattcga tgtgggcggt ggcgacgcgc cggtaatcgc ggttgaagaa
      601 ggcgaggaag ttgatggcga gataacgttt gtcgtaatcg gtcagcgtgc cgacgatgcc
      661 gaaatcgagg gcgatgtagc ggttgtcggc ggcaaccaaa atattgccgg ggtgcatatc
      721 cgcgtggaaa aagccgtcgc ggaagacttg cgtgaagaag atttccacgc cgtaatcggc
      781 gagtttgtgc aaatcgatgc cgtctgcttt gagtttggcg atgtcggaaa ccggcgtgcc
      841 gtccatccat tcgatggtca gcacgtcgct ggtgcagtag tcgtaaaaca ccttcggcac
      901 aatcagcata tcgctgtttt ggaaattgcg tccgagctgg ctggcattgg cggcttcgcg
      961 catcaagtcc aactcgtcgt gcagatattt gtcgaactcc gccaccactt cgcgcggctt
     1021 cagacgcttg ccgtcggcaa acagacgctc gacccagcct gcaccaaagc gcatcagcga
     1081 caaatcctgt tcgatcacgg gcaaaaggtt ggggcgcaaa actttaaccg ccacttgttc
     1141 gcccgaatgc aggcgggctt tgtgtacttg ggcgatggac gcgctggcga tgggctcggt
     1201 ttcaaattcc gcatacagct tttcgatgga ctgacccaac gatttttcga tttgttcacg
     1261 cgaaagccgc gcgtcaaaag gcggcacttt gtcttgcagc tttgccagtt cgacggcgta
     1321 atcgtgcgga atcaaatcgg ggcgtgtgga caaaacctgc ccgaacttga tgaaaatcgg
     1381 ccccaggctt tccaaggcaa ggcgcagacg gacagcaggc ggttcgtttt tcaatttgga
     1441 cgactgcggc atcattttca gcagagcgca tatccaaccg ctacccatca gcgaagcaca
     1501 cagacctgcc agccgatagc ggtaaaaagt cccgacaata accgtcaggc gtttcaacca
     1561 tttcatatat aaaatcccga atacagcatt tatcggacaa tcaaaaaatc ttaccgagca
     1621 gatagcccaa aaaggcaatc agcagcccga aaaccagcag tttgatattg cgggacggcg
     1681 gttcgggttg cgaaggtgcg gaaatatcat cttcccgccc gccgtgaagt tcaagatatt
     1741 cgggcatttc cccatccgcg ccttctcctt cgccctcccc ttcctccggc ggcacgaccg
     1801 tttccaactc catatatctc cgggtaataa tatccaaacc caaatagctg aaataatcat
     1861 gcaactcttc ccgtttcgcc caagtgcatt tttcctcaac cagcacttga ggtttggaat
     1921 cgagcgattc gatcaatcct tccgcctttt cctcgccgag attctcctta aggcactccc
     1981 ggataagccc gcgatccact tggggcgggt aagaaacata tacatcgtac aaatcgtttc
     2041 ggtaatcttt actcatctca atcataaggg aacaaatacg cctgataccg ttccgcccgt
     2101 tcctttcggg aaacccgatt catcttcaaa caatcggatt atatgtgaat tcatcccgat
     2161 aatatatttt ctaaaagtaa aaatgccgtc tgaacgttca gacggcatca cacaaaccaa
     2221 gcggtcatac ggaaaacgac gatccgcaac cgcaggttgt ttccgcattc ggattgcgga
     2281 tgacgaattg cgaaccctgc aaactttccg tatagtcgat ttccgcaccg accagatatt
     2341 gatagctcat cggatcgacc aaaaagacca aaccgttttt ctcaatttca aaatcgtcgt
     2401 cgtttttgat ttcgtcaaaa gtaaatccgt actggaaacc cgaacagccg ccgccattga
     2461 caaaaacccg caatttcaaa tcgggattgt tttcttcggc aatcaaatcg gcaactttgg
     2521 cacagcagct gtcagtaaaa ataatagggc tttcatccga catggtgtta ttccttaata
     2581 taatattttc tgcattctct ctcaaacccc cttcaaaggc aagcaggctt cgcctttgaa
     2641 aaaggaattg ttccaataga taagtttgac cgcctgcaat ttcaagcccc aaactcaaat
     2701 cgtcctgccg aaccactcga cacgcccgat aatctccaca tcatcggtca aatcgttcaa
     2761 attgatttca aaagcaggat aagcctcgtt tgcagaaatc acattgataa tcccgcccgg
     2821 tacaatctgt aaacgtttaa ccagcagatt ttcattaatc cgcaacacat acagaccgtc
     2881 cctcggcgta ttttcaccat gattgaccaa aatcgaatcg ccgtcattca aaaccccctc
     2941 catcgaatcc cccttgacgg aaattacaga caggtttttc gtatcgcggg taacataatt
     3001 ctcaatccaa tgccgtctga acgccattgt aaataccggt tcctcatgat cgacaaactg
     3061 cccgtatccc gcagccgccc gaatatcata tctcggcacg aagacaaact cgtccgtatc
     3121 gacttcattg cccaaagtat cgtaagcaag ggattttttt ggggcttcat ccggaaacgg
     3181 attaccctcc ccggtcagca gccaatcgat actacacccc ttcaactgct tgattttttt
     3241 caatgtttca gactttggca gaccgccttc attccatatc ctgctgaagc ccgcaatcgt
     3301 catttcaata tcggatgcga tttttgcctg cctcgcttcg cttttccata aaaaaaccag
     3361 tctgtcttta aaggtttcca taaatatcct tccactcccc ttcattcaat catctaaagt
     3421 aaacatgaaa tatattttac cttagctatc caaaaaacgg aacaattatg ataaactaaa
     3481 tttaaattat gatttttctt acttaataca taatttttca gatttttcca taggtttaat
     3541 cctgaattga cagaaaaagg atagaaaaat gaagaaaacc ggcaaatatc ttatctatac
     3601 tgcggcattt accgcattct gctttgcctt ccaagaaaac cgttctgaag ccaaacagcc
     3661 cgacatcact ttatccgcat ccctgtgcga acaattcaac atactgaacg ccaaagatat
     3721 ggatgcagaa caagtctccc tttccaaaga atgcgacatc atcgagtctt cacacgactg
     3781 ggaaaaagag tacggcaact tgaacgaaca ggaaatgctc gccggcatcg tctatgaata
     3841 aacctgcctg ccatttgaaa cattatgctt gaatgcattg gagccaaatg tattaaatca
     3901 aatataaaac caatatattc ataaagttat atacttatag ccatgctgta gcttgaaaca
     3961 gcccgccgcc cgccctattt acagcccatc gggacaaaat gttccaacat atttctcaaa
     4021 ataagcaaaa tcaaatagga ttatccacat ggcaaaagta atcggtatcg acttaggtac
     4081 aaccaactct tgtttggcca tttccgaaaa cggtcaaacc aaagtgatcg aaaacgcaga
     4141 aggcgcacgc accacgccgt ccgttatcgc ttatttggac ggcggcgaaa tcctcgtcgg
     4201 tgcgcctgcc aaacgccaag cggtaaccaa cgccaaaaac actatttacg ccgccaaacg
     4261 tttgatcggc cacaaatttg aagacaaaga agtccaacgc gacatcgaat ctatgccttt
     4321 cgaaatcatc aaagccaaca acggcgacgc atgggtaaaa gcacaaggca aagagctgtc
     4381 tcctcctcaa atttccgcag aagtcctgcg taaaatgaaa gaagccgccg aagcttactt
     4441 gggcgaaaaa gtaaccgaag ccgtgattac cgtccctgcc tacttcaacg acagccaacg
     4501 tcaagccacc aaagacgcag gccgtatcgc cggtttggac gtgaaacgca tcatcaacga
     4561 gccgaccgca gccgctttgg cattcggtat ggacaaaggc gacaacaaag accgcaaagt
     4621 agccgtatat gacttgggcg gcggtacttt cgatatttcc atcatcgaaa tcgccaacct
     4681 cgacggcgac aaacaattcg aagtattggc aaccaacggc gataccttct tgggcggtga
     4741 agacttcgac caacgcctca tcgaccacat catcgccgag ttcaaaaaag aacaaggcat
     4801 tgatttgaaa caagacgtga tggctctaca acgcctgaaa gaagctgccg aaaaagccaa
     4861 aatcgaattg tccagcggcc agcaaaccga aattaacctg ccgtacatca ccatggacgc
     4921 aaccggcccg aaacacttgg cgatgaaaat tacccgcgcc aaattcgaaa gcctggttga
     4981 agacctgatt acccgctcta tcgaaccttg caaaattgca ttgaaagatg ccggcttgag
     5041 caccggcgac atcgacgacg taatcttggt cggcgggcag tcccgtatgc cgaaagtaca
     5101 agaagccgtt aaagccttct tcggcaaaga accgcgcaaa gacgtgaacc ctgacgaagc
     5161 cgttgccgta ggcgcagcga tccaaggcga agtattgagc ggcggccgca gcgacgtatt
     5221 gctactggac gtaactcctc tgtctttggg tatcgaaacc atgggcggcg tgatgaccaa
     5281 actgattcag aagaacacca ccatcccgac caaagcgtcg caagtgttct ctaccgccga
     5341 agacaaccaa agcgcagtaa ccatccacgt actgcaaggc gaacgcgaac gcgcttctgc
     5401 caacaaatct ttgggtcagt tcaacttggg cgacatcgca cctgcaccgc gcggtatgcc
     5461 gcaaatcgaa gtaaccttcg acatcgacgc caacggcatc ctgcacgttt ccgccaaaga
     5521 caaaggcacc ggtaaagcag ccaacatcac catccaaggt tcttcaggtt tgagcgaaga
     5581 agaaatcgaa cgcatggtga aagatgccga agccaatgcc gaggaagata aaaaactgac
     5641 tgaattggtc gcttcccgca accaagccga agccctgatt cactctgtga aaaaatcttt
     5701 ggccgactac ggcgacaaac tcgacgcagc cgagaaagaa aaaatcgaag ccgcgctgaa
     5761 agaagccgaa gaagcagtta aaggcgacga caaagccgct atcgatgcca aaaccgaggc
     5821 gctgggcgca gccagccaaa aactggggga aatggtttac gctcaagcac aagctgaagc
     5881 ccaagcaggc gaaagcgaac aagccaatgc ttctgcaaag aaagacgatg atgtcgtaga
     5941 tgccgacttt gaagaagtaa aagacgacaa aaaataatta ataccgtctg aaaaaagcgc
     6001 gaaccgtttg attcgcgctt ttttcaattg agataaaaga ccatagcata acagaggttc
     6061 caagttcatc taccgtaata ttcccaaacc caagttccaa ctgttgtatc ggttctctgg
     6121 aaattttt
//
LOCUS       DAWISC010000127         5985 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWISC010000127 DAWISC010000000
VERSION     DAWISC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.127"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WIW87_001605"
     CDS             complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WIW87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ5174745.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQVQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WIW87_001606"
     CDS             complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WIW87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ5174746.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WIW87_001607"
     CDS             complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WIW87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ5174747.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDATVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVMSGLKDGDKVVVEGISIAGITGAKKVTPKEWASSENQA
                     AAPQSGVQTASEAKPASEAK"
BASE COUNT         1336 a         1707 c         1617 g         1325 t
ORIGIN      
        1 cattttattg cccgaccggt tatttgtcgg tttgggtatc ccgtttcaat ccgccgccga
       61 gtgccttgta caaatcggca aggttttcgg cgcgggtcag ttgtgccgac aaagccgcac
      121 cctccgccgc atagctgctg cgttccgcat cgagcaagtc gagcgcgccg gatacgccgt
      181 gcttgtaacg caggccgacc aagcgcaacg cttctttaga ggcgcggctt tgtttgctta
      241 aagcgtcata ggctttatcc agctgctcgc gcgccgccaa tgcgtttgcc acgtcttgaa
      301 atgcggattg gacggcggat tcataggcaa cgatttgtac ctgttggcgc agcttggcta
      361 catcaaggtt cgccttgttc gtaccccagg taaaaatcgg cagggtaata gacggcgcga
      421 acgaccaaac gcccgtgccg cttttgaaca acccacccaa ttcggcagaa cccgtaccga
      481 cggttccggt caggcggatg gatgggaaaa aggcggcgcg tgccgcaccg atattggcgt
      541 ttgcctgttt gagcgcgtgt tcggcagcac ggatatcggg acggtcgagc aatacttcgg
      601 aactcaaacc ggccggcagt ttttcaacaa aaaactgctt gtccagcggc aaaccggcag
      661 gcaggtcttc gggtatcggt tggttaatca aggttgccaa ggcattgcgc gcctgttcgc
      721 ggctgcgcgc ggcatgggca taatcggctt tggcagattc gatcagggct tcctgctgac
      781 gtagggcgac ggcggaaatc acgcctgcct tgtaacgtaa ttcggacagc ttgtaggttt
      841 cctcgcgcgt tttcaaaaca cgttgcgcca aagacatcgc ttcttcggcg taacgttcgt
      901 tgaaataggc tttggcaacg gtggcaatca ggctcaaatg tgccgcatcg cggttggcgg
      961 tgctggcgaa atagccttgc agtgccgcct cgctgctgct gcgtacacgc ccgaacagat
     1021 cgagttcgta agatgccgca cccagtccga ctttgtagct gctgcttaca ttgccgccgc
     1081 tcaagctgcc ttggcgcgag tcgttcgcat tggcggcaag cgtgggcagg aggttgttgc
     1141 gctcaatcat gtattgtttg cggtagattt cgctgttcaa tacggcggta cgcaaactgg
     1201 tattgcgctc gagtgcgatg tcgatcagct tttgcaggcg cgggtcggca aaatagtcat
     1261 gccaacctaa atcgacggcg cggatgccgc tgtcggcggt atcgtttttg aacgtttcgg
     1321 caacttcgac tttgggctgc tcgtattggg gaatcatggt gcaggcagac aatgcaaagg
     1381 ctgctgcaac agaagtcaag gtggttttca atgtagtatc cataaaaaag tcctgatgcc
     1441 gtctgaaaac ccgtgggcgt tcagacggca tggttgctta atgttggctg tcgtccgaac
     1501 cggtgatgcc cgcttcggcg gcgtgtttta ctgccatttc gtgttcgtgc gcggtttctt
     1561 tgaagaattt gcgcaccacc acatagaaaa gcggaacaag gaacacggac aagagcgtgc
     1621 cgatgagcat cccccagaat acggttgtac cgatggcgcg ctggctggca gaacttgcac
     1681 cgccggcaat atacagggga accacgccca aaataaaggc gaacgaggtc atgataatcg
     1741 gacggaaacg caggcgggcg gcttccaaag cggcttcaac cgcgcttttc ccttgcgctt
     1801 gaaggtcttt ggcaaattcg ataatcaaaa tcgcattttt cgcactcaaa cccatcacgg
     1861 taacgaaacc gacttgaaag tagatgtcgt tggcgaacga gggaacgctg cccaacagtc
     1921 cttcaaacag gttgcgcccg gttacgcccg cagccgcacc gatcaaaccc aacggaatca
     1981 caaggatgac cgccagcgga atcgaccagc tttcataaag cgcggcaagt accaaaaata
     2041 cggctgcaac cgccaaaccg tacaaaatca gggtttgcga gccgcctttt gcctcttcgc
     2101 gcgactgtcc gccccactcc aggctgtaac cgccgcccaa ttcgtcaacc attttttgaa
     2161 ccgccgccat agcctgcccg gtggaaacgc cggttgcagg cgaagcggac agcttcatcg
     2221 aaggataacc gttgaagcgt acgctctgtt ccgtaccgtt ttcccaagaa acagtagcaa
     2281 tggtggaaag cggtacggcg acgccggatt tgttcggcac ggtcaggttc aaaatatcgg
     2341 caggctgcat acgggcatcc tcgtcggcct gcaccatcac gcgttgcaga cggccttggt
     2401 tcgggaagtc gctgacataa gacgaactca gcgcgcttgc caatgcggtg cggatgtcgg
     2461 caaacgaaat gccttgcgcc gccgccgcgg cacggttgat gtcgattttc aactgcggcg
     2521 agtcttccaa accgccagca cggacggtgc tggggtcaaa caaaccgctg gcacgcattt
     2581 tctgaatcaa ctcgttgcgc ttcgccagca atgcggtatg gccggtattg ttgcggtctt
     2641 gcaggttgat gctcagaccc gaaccgttgc ccaactccag aatcggaggc gggacgacgg
     2701 cgatgccaaa accgtcttta agcgtcccca tcatcatacc cgtcagcttg ccggcaatcg
     2761 caacggcatc gctgccgggc gcggtacgct cgttccaatc tttcaatatg gcaaaaccca
     2821 tcgccatatt ctgaccgctg cccgaaaagc tgaagccgga aacggtaatg atgttttcta
     2881 tttcaggaat gcttttcgcc agttgggtaa cttgcgccaa agtcgcattg gtgcgctctt
     2941 gggtcgctcc tgcaggcagt tgcacgctga ccatgacgaa gccttggtct tcggtcggca
     3001 ggaatgaagt cggcaggcgc ataaacagga acacgcccac aaccgccaag ccgatataga
     3061 caaccatcat gcggaaagtc ttacgcagca ctttggcaac ccggccttcg taaccgtgcg
     3121 tccaactgtt gaatttcttg ttaaaccagc cgaagaaacc ttttttctct tcgtgatgcc
     3181 ctttcgggat tgtcttcaac attgtggcac acaaagcagg ggtaagggtc agcgcaagga
     3241 aggcggagaa tgcgattgat gacgccatcg tcagggcaaa ctgtttgtaa atattgcccg
     3301 tcgccccgct gaacatcgcc aacggtacga acacggaaat cagaacggcg gtaataccga
     3361 tgaccgcgcc cgaaatctga cccatcgctt ttttggtcgc ttctttgggc ggcaagcctt
     3421 cacccgccat aatgcgctcg acgttttcaa ccaccacaat cgcgtcatcg accacgatgc
     3481 cgatgaccaa aaccatcgca aacatggtca gtacgttaat cgacatgccc atataagaga
     3541 tgaaggcgaa accgcccaac agcgaaatcg gtacgacgat ggtcggaatc agcgtataac
     3601 ggatgttttg caggaagaga tacattacga caaacaccag caccatcgct tcgattaaag
     3661 tgtgaatcac tttttcaatc gaaatttcga cgaatttgga agtatcgtaa ggggttttcc
     3721 agctcatacc ctgaggaaag tatttttcca acgtcgccat gcgttcttta accgcctttg
     3781 ccgtcgccat cgcattgccg ctgttggaca gcatcaccgc cataccggtg gtatttacac
     3841 cgttcagacg ggttgaggaa gaatagtctt ccatacccag tccgaccctt gccacatcct
     3901 tcaggtaaac attagaaccg tcggtattgg cgcggaggat gacgttgccg aattcttctg
     3961 ccgtacccaa ctgcccttgc gccgttacgg tagccgtaac cgtctgtccg cgaacggcgg
     4021 gaagcgaacc gatagaaccc gctgaaatct ggacgttctg ggcggacagc gcgctgccaa
     4081 catcggcaaa cgacaaattg tagttttgca gtttcttagg atcaacccaa atccgcatcg
     4141 cgcgttgcgc gccgaacagg cgtacctgcc ccacgccttc gatacgctgc aactcgggaa
     4201 cgatattacg ctgcgcgtag tcgttcatct cttcggttga ctgcacatcc gacgaaagca
     4261 tcacaatcat caggaaattg gaacgcgcct tggatacggt tacgccgtat tgctggacag
     4321 ttgccggcag cgtgctcaat acttcggaaa gcttgttctg cacttccacc tgcgccagat
     4381 tctcgtcggt atcgggcgta aaggtcaggc tcacgctgcc gctgccgctc gaatcggcgg
     4441 aagtggacat ataatccaaa ccttccacgc cgttcatatt ccgctcgatc acggaaagca
     4501 cgctgtcttc cattacctgc gcggacgcgc ccggataagt ggccctcagg gtgatggtcg
     4561 gggcggcgac ggacggatat tgcgaaaccg gcaggctttt gatgccgaaa atacccgccg
     4621 caataatgaa aatcgaaata acccacgcaa aaatggggcg gtcgataaaa aatttagcca
     4681 tcgatgcctt ccttatttcg cttcagaagc aggtttggct tcagatgccg tctgaacgcc
     4741 ggattgaggc gcggcggctt ggttttcaga cgacgcccat tctttgggcg ttaccttttt
     4801 cgcacccgtt ataccggcga tactgatgcc ttccacaacc accttgtccc cgtccttcag
     4861 acccgacata acaatccaat tcgtaccctg ctgttgcgca accgttacct cgcggggttc
     4921 cataccgcct tgggcattca caatcatcac ggtatctttc gcaccgcgcg ttaccgcctg
     4981 ctgcggcaca acaaatgcgt tatccaccgc cacttggtcc atcagcacgc gcacatacag
     5041 accgggcatc aagatattct gatcgttcgg tacggcggcg cgcagggtaa tctgaccggt
     5101 cgattcgttg acggccggat cggcaaacag caggcggcct ttttcagggt aaactgtgcc
     5161 gtcgtcaaat ttgatgccga ccgcaatcac accatccgcc gccagcagtt tgccttcggc
     5221 tatctgacgg cgcaatttca tcacttcgga tgcagactgg gtaacgttca catacatcgg
     5281 attggtttgg cggatggtcg ccagtacggt cgcatcgcca gcgttcagca acgtaccttc
     5341 ggaaactttg gactgaccga taaagccgga aatcggcgcg gtaatgcgcg aacggttcag
     5401 gctgatgccg gcggatttga ttgccgcctg cgccgcttta acgcctgcct cggcagaacg
     5461 tttcgccgtt accgcagcat cgtattcctg ccggctgacg gcttcggcgg caaccaaagg
     5521 cttgtatcgc gccaaatccg catccgcttt ggcaagcgtt gcctgagccg ttgccagttg
     5581 cgcgcgcgcg ctttccagac ctgcttcata agtggaactg tcgatctgat acagcggctg
     5641 tccggcacgg acataactgc cttcttggaa caggcgtttt tggatgatgc cgccgacttg
     5701 ggcgcggaca tcggcggtac gcagcgattc caaacgcccc ggcaactcga cggtcaatgc
     5761 gacggtttgc ggatggacgg ttacgacacc gacgacgggc gcaggggctt cccgaccagc
     5821 aggctgcccg ccctgcgccg cgtctccgcc tttaccgcaa gacgacagta ccaatgcaac
     5881 ggcggcagcc aacgcggccg cacgcatcgc cttaaaagca taaaaagcca ttatttatcc
     5941 tatctgtctg gtttgatgta aagggttttg ccaatcaaca ggcat
//
LOCUS       DAWISC010000128         5943 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWISC010000128 DAWISC010000000
VERSION     DAWISC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.128"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(125..1114)
                     /gene="pdxA"
                     /locus_tag="WIW87_001608"
     CDS             complement(125..1114)
                     /gene="pdxA"
                     /locus_tag="WIW87_001608"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ5174748.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1274..2701
                     /locus_tag="WIW87_001609"
     CDS             1274..2701
                     /locus_tag="WIW87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5174749.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            3026..4921
                     /gene="mnmG"
                     /locus_tag="WIW87_001610"
     CDS             3026..4921
                     /gene="mnmG"
                     /locus_tag="WIW87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ5174750.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            4990..5574
                     /gene="rnhB"
                     /locus_tag="WIW87_001611"
     CDS             4990..5574
                     /gene="rnhB"
                     /locus_tag="WIW87_001611"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ5174751.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT         1395 a         1748 c         1572 g         1228 t
ORIGIN      
        1 ccaatggtct ggattcccgc ctgcgcggga atgacgattt ggaaattacc cgaaacccaa
       61 aaacaactga aaccgaacag accggattcc cgcctgcgcg gaaatgacgg gtcttttatc
      121 atctttaaag gctgccgcgc gccatctcga cggcggtctc cacggcagtt atcaggctgc
      181 cggaatccgc cctgccggtt gccgccaaat caagcgcggt gccgtgatcg acggaggtgc
      241 ggataaaggg caggccgagc gtgatgttca cgccctgtcc gaagctgtgg tatttcaaca
      301 cgggcagccc ttggtcgtgg tacatcgcca atacggcatc cgcaccttcg agcataaacg
      361 gttggaacaa ggtatccgcc ggatacgggc cggcgaggtt tatcccttcg cggcgcaggt
      421 tttcgagcgc ggggataatg gtgtcggttt cttcgtgtcc gaggtgtccg ccttcgccgg
      481 cgtgggggtt gagtccggcg actaggattt tgggattttt gatgccgaat ttgtgtttta
      541 agtcgtgatg caaaatgcgt gcgacgcttt caatcagcgg ttgcgtgatg gcggcggcaa
      601 cgtctttcag cggcaggtgg gtcgttacga gggcgacgcg caggcctttg ccggcaagca
      661 tcatcacgac ctgccccgtg ccgctttttt ccgccagata ttcggtgtgt ccgctgaaaa
      721 aacctgtgct tgcgcgcgcg tcgttgatga tgcctttgtg cagcggcgcg gtaacgatgc
      781 cgtcgaaaat gccgtctgaa atgcctgcga gcgcggtgtc caaaagttgc agcacatagg
      841 cggcgttggc gggattgagt ttgcccgcct caaccgcttc gacggcaggg atgtgcagca
      901 cttccagctc gccgtatgcc gcgccgcctg attctggatc gaagtcgcgc aggacgacgc
      961 ttttgcccaa ggcttcggcg cgcgcgcgca ataggttttt gtcgcccaat accgcgcagc
     1021 ggcagggcag gcgtgcaaac gccaagtcca aacaaatatc ggggccgatg ccggcaggct
     1081 cgccggaagt aacggcaaaa acaggctgtt tcatcgtgtt tgctccaaac aaaatgcgat
     1141 tctaacgccg cagccgcgcg gcgatgtaaa tttttctgat tttgttgaca atctgctaga
     1201 atgggcgttt acaaaattta aaccctgctt gcataccgcc aatatgtgcg agtttcaact
     1261 ttaaggaagc gatatgaacg agaactttac cgaatggctg cacggctggg tcggcgccat
     1321 caacgatccg atgtggtcat acttggttta tatgcttttg ggtacggggc ttttcttcac
     1381 cgtaaccacg ggctttgtcc aattccgcct gttcgggcgc agcatcaaag aaatgctcgg
     1441 cggccgcaaa cagggggacg accctcacgg catcacgccg tttcaggcat ttgtaaccgg
     1501 ccttgccagc cgcgtgggcg tgggcaatat cgcgggcgtg gccatcgcca tcaaagtcgg
     1561 cggaccgggc gcggtgtttt ggatgtgggt aaccgcctta atcggtatga gttcggcgtt
     1621 tgtcgaatct tcgctggcgc agctctttaa agtccgcgac tacgacaacc accatttccg
     1681 gggcggccct gcctactaca tcactcaagg gctggggcag aaatggctgg gcgtgttgtt
     1741 cgccctgagc ctgattttct gtttcggctt tgtgtttgaa gcggttcaga ccaataccat
     1801 tgccgatacc gtcaaagcgg cgtggggttg ggagcctcat tatgtcggcg tcgccctggt
     1861 gattttaacc gcgccgatta tcttcggcgg catcaggcgc atatctaaag cggcggaaat
     1921 cgtcgtcccc ctgatggcgg ttttgtacct ctttatcgcg cttttcatca ttttgaccaa
     1981 tattccgatg attccggacg tgttcggtca gattttttcg ggcgcgttca aattcgacgc
     2041 ggcagcaggc ggcttactcg gcggtctgat ttcgcaaacg atgatgatgg gcatcaaacg
     2101 cggcctgtat tccaacgagg cgggtatggg ttccgcgccg aacgccgccg ccgccgccga
     2161 agtgaaacac cctgtttcgc aaggtatgat tcaaatgctg ggcgtgtttg tcgataccat
     2221 catcgtttgt tcttgcaccg ccttcatcat cttgatttac caacagcctt acggcgattt
     2281 gagcggtgcg gcgctgacgc aggcggcgat tgtcagccaa gtggggcaat ggggcgcggg
     2341 cttcctcgcc gtcatcctgt ttatgtttgc cttttccacc gttatcggca actatgccta
     2401 tgccgagtcc aacgtccaat tcatcaaaag ccattggctg attaccgccg ttttccgtat
     2461 gctggttttg gcgtgggtct atttcggcgc ggttgccaat gtgcctttgg tctgggatat
     2521 ggcggatatg gcgatgggca ttatggcgtg gatcaacctt gtcgccatcc tgctgctctc
     2581 gccactggcg tttatgctgc tgcgcgatta caccgccaag ctgaaaatgg gcaaagaccc
     2641 cgagttcaaa ctttccgaac atccgggcct gaaacgccgt atcaaatccg acgtttggta
     2701 aatcccgccc ttaccggagc cgcttccccg cgaagcggct tttccctttc cgcacactgt
     2761 aaaaacaggg cgaacaagcg tacaatccca accctttact tttgaatcca tttcgttttt
     2821 cagacggcat attgaatata gtggtttaac aaaaatcggg acaaggcggc gaagccgcag
     2881 acagtacaga tagtacagaa ccgattcact tggtacttca gcaccttaga gaatcgttct
     2941 ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat aaatgccgtc
     3001 tgaaacaccg tcaggcaata cacacatgac ccacatgatt taccccaaaa cctacgacgt
     3061 tatcgtcgtc ggcggcggac acgccggcac agaagccgcg ctcgccgccg cccgtatggg
     3121 cgcgcagacg cttttgctca cacacaatat tgaaacgctc ggacaaatgt cgtgcaaccc
     3181 ttccatcggc ggcatcggca aagggcattt ggtgcgcgaa ctcgacgcgc tcggcggcgc
     3241 gatggcgttg gcaaccgaca aatccggtat ccagttccgc cgcctgaacg ccagcaaagg
     3301 cgcggcagtg cgtgccacgc gcgcgcaggc ggaccgcatc ctgtacaaag ccgccatccg
     3361 cgaaatgttg gaaaaccaag aaaacctcga ccttttccaa caagccgtcg aagacgtaac
     3421 gctcgacggc gaacgcatca gcggcgtaat taccgcgatg ggcgtggagt ttaaagcacg
     3481 cgccgtcgtg ttgaccgcag gcacgttttt gtccggcaaa atccacatcg gtttggaaaa
     3541 ctacgaaggc ggacgcgccg gcgaccccgc cgccaaatcg ttgggcggac gtttgcgcga
     3601 attgaagctg ccgcaaggcc gtctgaaaac cggcacgccg ccgcgtattg acggacgcac
     3661 gattgacttc tcccaactga ccgaacagcc cggcgacacg cccgttcccg tcatgtccgt
     3721 gcgcggcaac gccgatatgc acccgcgcca agtgtcctgc tggattacgc ataccaacac
     3781 gcaaacccac gacatcatcc gctcaggctt cgaccgcagc ccgatgttta ccggcaaaat
     3841 cgaaggcgtg ggtccgcgtt attgtccgtc tatcgaagac aaaatcaacc gcttcgccga
     3901 caaagacagc caccagattt tcctcgaacc cgaaggtctg accacgcacg aatactatcc
     3961 taacggtatc tccaccagcc tgccgttcga catccaaatc gcgctcgtcc gcagtatgaa
     4021 aggtttggaa aacgcccata tcctgcgccc cggctacgcc atcgaatacg actacttcga
     4081 tccgcgcaac ctcaaagcaa gcctcgaaac caaaaccatt gccggattgt ttttcgccgg
     4141 gcaaatcaac ggtacgaccg gctacgaaga agccgccgcg caaggtttat tggcaggcgc
     4201 gaacgccgtg caatatgtgc gcggacaaga cccgctcctg ctgcgccgcg aacaagccta
     4261 cctcggcgta ttggtggacg acctcatcac caaaggcgtg aacgaaccct accgaatgtt
     4321 caccagccgc gccgaatacc gcctgcaact cagggaagac aacgccgaca tgcgcctgac
     4381 cgaagacggc tacaaaatcg gcttggtgtc cgaagcgcaa tggcgcatgt tcaacgaaaa
     4441 acgcgaagcc gtcgaacgcg aaatccaacg tttgaaaaca acgtggtaca cgccgcaaaa
     4501 actcgccgaa ggcgaacaaa tccgtgtgtt cggacaaaaa ctcagccgcg aagccaacct
     4561 gcacgacctc ctgcgccgcc caaacctcga ctacgccgcg ctgatgacgc tcgaaggcgc
     4621 gatgccgtct gaaaacctct ccgccgaagt catcgaacaa gtcgaaatcc aagtcaaata
     4681 ccaaggctat atcgaccgcc aaaacgaaga aatcgacagc cgccgcgaca tcgaaacctt
     4741 aaaactgccc gacggcatcg attacggcaa agtcaaaggc ttgtcggcag aagtgcagca
     4801 aaagctcaac cagcacaaac ccgaaaccgt cggacaagcc agccgcattt ccggcgtaac
     4861 ccctgcggca gtggcattgc tgatggtgca tttgaagcgc gggtttaaag acgcgaaata
     4921 aacacatcgg cgcgatgccg tctgaaaccc ttttcagacg gcattcccac catcccgaca
     4981 ggaaacatca tgcacatact gaccgccggc gtggacgagg caggacgcgg acctttagtc
     5041 ggcagcgtgt ttgccgccgc cgtcatcctt ccggaaacat tcgacctgcc cggactgacc
     5101 gactccaaaa aactcagcga gaaaaaacgc gacgcgcttg ccgaaatgat aaaaaatcag
     5161 gcggttgagt ggcacgttgc cgccgcctcg cccgaagaaa tcgccagcct caacatcctg
     5221 cacgccacca tgctcgcgat gaaacgcgcc gttgacggct tggctgtgcg tcccgaaaaa
     5281 atattcatcg acggcaaccg cattcctgaa catttgaaca tccctgccga agccgtcgtc
     5341 aaaggcgaca gcaaaatcat cgaaatctcc gccgcatccg ttttggcaaa gaccgcacgc
     5401 gatgcggaaa tgtacgcact ggcgcaacgc catccccaat acggtttcga caaacacaaa
     5461 ggttacggca cgaagcagca tttggaagcc ctcaaacaat acggcgtgct gcccgaacac
     5521 cgccgcgact tcgcccccgt cagaaacctg ctcgcgcagc aggccttgtt ttaaaccggc
     5581 acaaaaatgc cgtctgaagc cttcagacgg catttccggc atcgcaactc caaagagaaa
     5641 gaacccaaac cgtcattccc gcgaaaaata gaaaatcaaa aaaaaaacct aaaatccgtc
     5701 attcccgcgc aggcgggaat ccaatccgtc cggtttccgt tttttttttg aatttcaggt
     5761 aacttccaaa ccgtcattcc cgcgaaagcg ggaatctaga aactcaaagc tgcaagaatt
     5821 tatcaaaaat gactgaagct caaaaaaccg gattcctacg aaaacaggaa tccggagtct
     5881 cagggctggc aaaaccgttt tacccgataa gtttccgtac cgacagacct agattcccgc
     5941 ctt
//
LOCUS       DAWISC010000129         5904 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWISC010000129 DAWISC010000000
VERSION     DAWISC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.129"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="WIW87_001612"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="WIW87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ5174752.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WIW87_001613"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WIW87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ5174753.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="WIW87_001614"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="WIW87_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ5174754.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WIW87_001615"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WIW87_001615"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ5174755.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLIRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3331..3407)
                     /locus_tag="WIW87_001616"
     tRNA            complement(3331..3407)
                     /locus_tag="WIW87_001616"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3371..3373),aa:Val,seq:gac)
     gene            3586..4701
                     /gene="tgt"
                     /locus_tag="WIW87_001617"
     CDS             3586..4701
                     /gene="tgt"
                     /locus_tag="WIW87_001617"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ5174756.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            4711..5793
                     /locus_tag="WIW87_001618"
     CDS             4711..5793
                     /locus_tag="WIW87_001618"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ5174757.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
BASE COUNT         1465 a         1558 c         1396 g         1485 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcaatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgtacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcacggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacctaaa
     1741 atggctcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaatc aacaattcat tgaacgcacg agcctcgctg
     2101 acgatttgat cttcggtaca gaaaatatgc gcatcatcct gcacaaaacc gcgaacccgc
     2161 atcagaccgt gcagcgcacc gctcggctca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattatct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccatgat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcaggtgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctggtga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg cgcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgtcgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaata ttcaacatca aacgctccga aagattaaaa
     3301 aataacggca taaatgccgt tatgggaatt tggtaggcac gattggattc gaaccaacga
     3361 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cgtgccttca aagaattttt
     3421 gcattttatc gggtcgtttt aatttttgca agaggtatgt gcgtttcttt atgcaacatt
     3481 gcggcaagat ttggggatgt cgtctgaaaa accgtgggaa atcatttata atgccgtctt
     3541 ttaaaatata tttcgacccc tgtcgcccaa ctgacattca atcttatgct taaatttacc
     3601 ttacacaaaa aagacggtct cgcccgacgc ggcacgctgg agttgaacca cggcaaaatt
     3661 gaaacgccgg tatttatgcc tgtcggtact tacggttcgg ttaaggcgat gaacccgcaa
     3721 aacctgcacg atatcaaggc acaaattatt ttgggcaaca cttatcattt gtggctgcgt
     3781 ccgggtttgg aagttatcgg gcaattcggc ggcctgcacg gttttatcgg ctgggacaaa
     3841 ccgattctga ccgactcggg cggttttcag gttttttctt tgtcggatat gcgcaagctg
     3901 acggaagaag gctgtacgtt caaaagcccg attaacggcg acaagctgtt tttatcgcct
     3961 gaaatttcca tgaaaatcca aacggttttg aactcggata tcgcgatgca gttggacgaa
     4021 tgcacgccgg gcgaggcgac gcgcgaacag gcgcgaaaat cgctgcaaat gagcctgcgc
     4081 tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa acccgaacgc gctgttcggc
     4141 atcgtgcaag gcgcgatgta tgaggatttg cgcgaagaat cgctgcgcgg tttggaacag
     4201 tttgattttc ccggccttgc cgtcggcggt ttgtccgtcg gcgaacccaa gcccgaaatg
     4261 taccgtatgc tgcgcgccgt cggtccgatt ctgccggaac acaagccgca ttacctgatg
     4321 ggcgtaggca cgccggaaga cctcgtgtac ggcgtggcac acggcatcga tatgttcgac
     4381 tgcgtgatgc ccacccgcaa tgcacgcaac ggctggctgt ttacccgttt cggcgatttg
     4441 aaaatcaaaa acgccaaaca caagctcgac aagcgtccga tagacgaaag ctgcacctgc
     4501 tacgcctgcc aaaacttcag ccgcgcctac ctgcaccatc tgcaccgcac cggcgaaatc
     4561 ttgggcgcac agttgaacac catccacaat ctgcatttct accaagtcat tatggcggaa
     4621 atgcgcgaag ccgtcgaaca aggcaaattt gccgactggc aggcgcgatt ccacgaaaac
     4681 cgcgcacgcg gcacagactg attccctgca atgccgtctg aagccgcttc agacggcatt
     4741 ttgccgcttt accctacccg cactattttt cagaaaccga tcatgcttcc attctttcca
     4801 gaaccctccc tttcctatac gcaacaaaac cgtaccgccg ttttgctgtt gaacctcggc
     4861 acgcccgacg cgccgaccgc gcaggcggta cgaccttatc tgaagtcctt tctgaccgac
     4921 cggcgcgtcg tcgaactgcc caaatggctg tggtatccca ttctgcatgg tctggtactg
     4981 acgctccgcc ccaaaaaaag cgcgcacgcc tatgaaaaaa tctggtttaa ggaaggttcg
     5041 cccttagaag tttacacagc acgccaagct gccgcgcttg ccaaacgtat gcccgacctc
     5101 atcgtccgcc acgccatgac ctacggcaat ccttcggtgg cagacgtatt gtcggaactg
     5161 aaggcgcaag gcgcgggcag actgctggtc atcccgatgt acccgcaata tgccgcttcc
     5221 agcagcggag cggcagtaga taaggtgtgc gaacaattac tgctgcaacg gaaccaaatg
     5281 agcgtccgca ccgtttcgcg cttttatgac gataccggct acattgatgc gatgaaaaac
     5341 cacatcctcc gatattgggc ggaacacggg cgcgggaaaa aactgatgtt gagttttcac
     5401 ggcgtaccgc agaagcacca cgacctcggc gacccctatc ccgacgagtg ccgccacacc
     5461 gccaaactgc ttgccgaagc actggaactg accgaagacc aatatgtcgt atcgttccaa
     5521 agccaattcg gcagggcaaa atgggttaca ccgagcacgc aggatttgtt cggcaaactg
     5581 cccaaacagg gggtaaccga gctggacgta ttttgcccgg gctttttggc agactgtttg
     5641 gaaaccatgg aagaaatcgc cctgatgggg cgggaacagt tttatgaagc aggcggcaaa
     5701 agctaccgct acatcccctg cctcaacgac aaccccgact ggatagatgc actcgtcgca
     5761 cttgccgaag aaaaccttgg cagctggcgt tgaaactgtt tgacccatgt ctaaaatgcc
     5821 gtctgaacgc cgtgttcaga cggcattttc ccgctacttt agcgtatgat ggcatctatg
     5881 ctggacaaca acatcaagtg caac
//
LOCUS       DAWISC010000130         5890 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWISC010000130 DAWISC010000000
VERSION     DAWISC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.130"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..264)
                     /locus_tag="WIW87_001619"
     CDS             complement(<1..264)
                     /locus_tag="WIW87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ5174758.1"
                     /translation="MLCPEKMSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEA"
     gene            complement(322..435)
                     /gene="rrf"
                     /locus_tag="WIW87_001620"
     rRNA            complement(322..435)
                     /gene="rrf"
                     /locus_tag="WIW87_001620"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(532..3423)
                     /locus_tag="WIW87_001621"
     rRNA            complement(532..3423)
                     /locus_tag="WIW87_001621"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3829..3904)
                     /locus_tag="WIW87_001622"
     tRNA            complement(3829..3904)
                     /locus_tag="WIW87_001622"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3869..3871),aa:Ala,seq:tgc)
     gene            complement(3910..3986)
                     /locus_tag="WIW87_001623"
     tRNA            complement(3910..3986)
                     /locus_tag="WIW87_001623"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3950..3952),aa:Ile,seq:gat)
     gene            complement(4088..5628)
                     /locus_tag="WIW87_001624"
     rRNA            complement(4088..5628)
                     /locus_tag="WIW87_001624"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1289 a         1717 c         1257 g         1627 t
ORIGIN      
        1 cgcctccaac tcctgctgcg aagccgcatt gatgtttacc gccgcaaggg agaaggcgca
       61 ggagaacagc atacagaaca gcacgaacat tttcttcatg gtttttcctt taagggttgc
      121 aaacaataaa ccgcatcttg cgacgataaa acgagtcatt ctaaaatgaa tatcccaaag
      181 tttcaagccg ttcctccgca aacccgaccg gacaccgtac ggatgccgtc ccgccatcac
      241 cgacattttt tccgggcaaa gcaaacattt tttccgggca aagcaaaaac ccccgaataa
      301 tcgggggttt tctgaatggg tgtttggcag tgacctactt tcgcatggaa gaaccacact
      361 atcatcggcg ctgagtcgtt tcacggtcct gttcgggatg ggaaggcgtg ggaccaactc
      421 gctatggccg ccaaacttaa actgttacaa atcggtaaag ccttaatcaa tatattcggt
      481 aatgactgaa tcagtcagta agcttttatc tcttgaagtt cttcaaatga tagagtcaag
      541 cctcacgagc aattagtatg ggttagcttc acgcgttacc gcgcttccac accccaccta
      601 tcaacgtcct ggtctcgaac gactctttag tgcggttaaa ccgcaaggga agtctcatct
      661 tcaggcgagt ttcgcgctta gatgctttca gcgcttatct cttccgaact tagctacccg
      721 gctatgcaac tggcgttaca accggtacac cagaggttcg tccactccgg tcctctcgta
      781 ctaggagcag cccccgtcaa acttccaacg cccactgcag atagggacca aactgtctca
      841 cgacgtttta aacccagctc acgtaccact ttaaatggcg aacagccata cccttgggac
      901 cgactacagc cccaggatgt gatgagccga catcgaggtg ccaaactccg ccgtcgatat
      961 gaactcttgg gcggaatcag cctgttatcc ccggagtacc ttttatccgt tgagcgatgg
     1021 cccttccata cagaaccacc ggatcactat gtcctgcttt cgcacctgct cgacttgtcg
     1081 gtctcgcagt taagctacct tttgccattg cactatcagt ccgatttccg accggaccta
     1141 ggtaaccttc gaactcctcc gttacgcttt gggaggagac cgccccagtc aaactgccta
     1201 ccatgcacgg tccccgaccc ggatgacggg tctgggttag aacctcaaag acaccagggt
     1261 ggtatttcaa ggacggctcc acagagactg gcgtctctgc ttctaagcct cccacctatc
     1321 ctacacaagt gacttcaaag tccaatgcaa agctacagta aaggttcacg gggtctttcc
     1381 gtctagcagc gggtagattg catcttcaca accacttcaa cttcgctgag tctcaggagg
     1441 agacagtgtg gccatcgtta cgccattcgt gcgggtcgga acttacccga caaggaattt
     1501 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcga tccgatgctc
     1561 tcacatcttc aattaacctt ccagcaccgg gcaggcgtca caccctatac gtccactttc
     1621 gtgttagcag agtgctgtgt ttttaataaa cagtcgcagc cacctattct ctgcgaccct
     1681 ccggggctta cggagcaagt ccttaacctt agagggcata ccttctcccg aagttacggt
     1741 atcaatttgc cgagttcctt ctcctgagtt ctctcaagcg ccttagaatt ctcatcctgc
     1801 ccacctgtgt cggtttgcgg tacggttcga ttcaaactga agcttagtgg cttttcctgg
     1861 aagcgtggta tcggttgctt cgtgtccgta gacactcgtc gtcacttctc ggtgttaaga
     1921 agacccggat ttgcctaagt cttccaccta ccggcttaaa caagctattc caacagcttg
     1981 ccaacctaac cttctccgtc cccacatcgc atttgaatca agtacaggaa tattaacctg
     2041 tttcccatcg actacgcatt tctgcctcgc cttaggggcc gactcaccct acgccgatga
     2101 acgttgcgca ggaaaccttg ggctttcggc gagcgggctt ttcacccgct ttatcgctac
     2161 tcatgtcaac attcgcactt ctgatacctc cagcacactt tacaatgcac cttcatcagc
     2221 ctacagaacg ctcccctacc atgccggtaa accggcatcc gcagcttcgg ttatagattt
     2281 gagccccgtt acatcttccg cgcaggacga ctcgaccagt gagctattac gctttcttta
     2341 aatgatggct gcttctaagc caacatcctg gctgtctggg ccttcccact tcgtttacca
     2401 cttaatctat catttgggac cttagctggc ggtctgggtt gtttccctct tgacaacgga
     2461 cgttagcacc cgctgtctgt ctcccgagga accacttgat ggtattctta gtttgccatg
     2521 ggttggtaag ttgcaataac cccctagcca taacagtgct ttacccccat cagtgtcttg
     2581 ctcgaggcac tacctaaata gttttcgggg agaaccagct atctccgagt ttgtttagcc
     2641 tttcacccct atccacagct catccccgca ttttgcaaca tgcgtgggtt cggtcctcca
     2701 gtacctgtta cggcaccttc aacctggcca tggatagatc actcggtttc gggtctacac
     2761 ccagcaactc atcgccctat taagactcgg tttccctacg cctcccctat tcggttaagc
     2821 tcgctactga atgtaagtcg ttgacccatt atacaaaagg tacgcagtca caccactagg
     2881 gcgctcccac tgtttgtatg catcaggttt caggttctgt ttcactcccc tcccggggtt
     2941 cttttcgcct ttccctcacg gtactggttc actatcggtc gatgatgagt atttagcctt
     3001 ggaggatggt ccccccatat tcagacagga tttcacgtgc cccgccctac ttttcgtacg
     3061 cttagtaccg ctgttgagat ttcgaatacg ggactgtcac ccactatggt caagcttccc
     3121 agcttgttct tctatctcga cagttattac gtacaggctc ctccgcgttc gctcgccact
     3181 acttgcggaa tctcggttga tttcttttcc tccgggtact tagatggttc agttctccgg
     3241 gttcgcttct ctaagtctat gtattcaact taggatactg cacagaatgc agtgggtttc
     3301 cccattcgga catcgcggga tcattgcttt attgccagct cccccgcgct tttcgcaggc
     3361 ttacacgtcc ttcgtcgcct atcatcgcca aggcatccac ctgatgcact tattcacttg
     3421 actctatcat ttcaagaact tctttgactt tgcctaacat tccgttgact agaacatcag
     3481 acttgaattt cctactttga taaagcttac tgctttgttg tgtcttaatc ctgccttttg
     3541 tgtttcagga ttaagtcgat acaatcatca cccaaatact gtgtttgttt tcttttctct
     3601 tgcgagagat ttttatcctt tgcaaagaat aaaaaatcaa aacaaacgct ttgtctttgt
     3661 ttgttgattt cggctttcca atttgttaaa gatcgatgcg ttcgatattg ctatctactg
     3721 tgcaaatcaa aacgagctga ttattatatc agcattttgt tcttggtcaa gtgtgacgtc
     3781 gccctgaatg gattctgttc cattcttccg ttttgatttg tacagtattg gtggaggcaa
     3841 acgggatcga accgatgacc ccctgcttgc aaagcaggtg ctctaccaac tgagctatgc
     3901 ccccgttctt ggtgggtctg ggaggacttg aacctccgac cccacgctta tcaagcgtgt
     3961 gctctaacca gctgagctac aaacccggat tctcttctta agcgaatctt gccttcactc
     4021 aagcttcttc cgcatctttt tcagtttacc gataagtgtg aatgcctaaa gcctcttctt
     4081 tctctagaaa ggaggtgatc cagccgcagg ttcccctacg gctaccttgt tacgacttca
     4141 ccccagtcat gaagcatacc gtggtaagcg gactccttgt ggttatccta cctacttctg
     4201 gtatccccca ctcccatggt gtgacgggcg gtgtgtacaa gacccgggaa cgtattcacc
     4261 gcagtatgct gacctgcgat tactagcgat tccgacttca tgcactcgag ttgcagagtg
     4321 caatccggac tacgatcggt tttgtgagat tggctccgcc tcgcggcttg gctaccctct
     4381 gtaccgacca ttgtatgacg tgtgaagccc tggtcataag ggccatgagg acttgacgtc
     4441 atccccacct tcctccggct tgtcaccggc agtctcatta gagtgcccaa ctgaatgatg
     4501 gcaactaatg acaagggttg cgctcgttgc gggacttaac ccaacatctc acgacacgag
     4561 ctgacgacag ccatgcagca cctgtgttac ggctcccgaa ggcactcctc cgtctccgga
     4621 ggattccgta catgtcaaga ccaggtaagg ttcttcgcgt tgcatcgaat taatccacat
     4681 catccaccgc ttgtgcgggt ccccgtcaat tcctttgagt tttaatcttg cgaccgtact
     4741 ccccaggcgg tcaatttcac gcgttagcta cgctaccaag caatcaggtt gcccaacagc
     4801 taattgacat cgtttagggc gtggactacc agggtatcta atcctgtttg ctacccacgc
     4861 tttcgggcat gaacgtcagt gttgtcccag gaggctgcct tcgccatcgg tattcctcca
     4921 catctctacg catttcactg ctacacgtgg aattctacct ccctctgaca cactcgagtc
     4981 acccagttca gaacgcagtt cccgggttga gcccggggat ttcacatcct gcttaagtaa
     5041 ccgtctgcgc ccgctttacg cccagtaatt ccgattaacg ctcgcaccct acgtattacc
     5101 gcggctgctg gcacgtagtt agccggtgct tattcttcag gtaccgtcat cagccgctga
     5161 tattagcaac agccttttct tccctgacaa aagtccttta caacccgaag gccttcttca
     5221 gacacgcggc atggctggat caggcttgcg cccattgtcc aaaattcccc actgctgcct
     5281 cccgtaggag tctgggccgt gtctcagtcc cagtgtggcg gatcatcctc tcagacccgc
     5341 tactgatcgt cgccttggta ggcctttacc ccaccaacta gctaatcaga tatcggccgc
     5401 tcgaatagcg caaggcccga aggtcccctg ctttctctct caagacgtat gcggtattag
     5461 ctgatctttc gatcagttat cccccactac tcggtacgtt ccgatatgtt actcacccgt
     5521 tcgccactcg ccacccgaga agcaagcttc tctgtgctgc cgtccgactt gcatgtgtaa
     5581 agcatgccgc cagcgttcaa tctgagccag gatcaaactc ttatgttcaa tctctaactt
     5641 tttaacttct ggtctgcttc aaagaaacca acaggacaat gttcaaaaca ttatcttgtc
     5701 tgtctttcaa acagtgtgag gctcaaggca ctcacactta tcggtaatct gttttgttaa
     5761 agagcgtttg aattataaag tatttcttcc gcctgtcaag atatctctcg atatccccaa
     5821 cattctgtgc tatacttttc agttcgtccg ccgcttctgc agcagcgaag aaccgaacta
     5881 tacgcccaca
//
LOCUS       DAWISC010000131         5883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWISC010000131 DAWISC010000000
VERSION     DAWISC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.131"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            131..565
                     /locus_tag="WIW87_001625"
     CDS             131..565
                     /locus_tag="WIW87_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ5174759.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WIW87_001626"
     CDS             complement(578..1441)
                     /locus_tag="WIW87_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ5174760.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WIW87_001627"
     CDS             complement(1449..2066)
                     /locus_tag="WIW87_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HEZ5174761.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHFMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2842)
                     /locus_tag="WIW87_001628"
                     /pseudo
     CDS             complement(2229..2842)
                     /locus_tag="WIW87_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            3048..4820
                     /locus_tag="WIW87_001629"
     CDS             3048..4820
                     /locus_tag="WIW87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ5174762.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4817..5473
                     /locus_tag="WIW87_001630"
     CDS             4817..5473
                     /locus_tag="WIW87_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ5174763.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1446 a         1654 c         1363 g         1420 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcaga ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gagaaattcg ttcaattcca
     1441 tctctgcctc actttgcata tctttgcgcc atcagccgtt cgacggtatc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg aagtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccggccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagccgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttaccccca aaagagcaag ccctttgtcc tcgcctccca
     2761 tacggctcgc ctgaccgccg gccagtatca gggcaaaagt tttcattgcg gatgttctct
     2821 tggaaaagtt cgaggttttc atgattgcag tctgccgttc ccaatgactc aaaatgccgt
     2881 ctgaagcaga cggcaaataa attcatatta tctgaatttt atcataacat gatttaacac
     2941 tgaagcggtg cggattaagt tttccttaac caattctttc tgagcagttg tatctaattc
     3001 caaagaatga tattgtttgc attatttgga acaatttttc gccgagcatg atactgccag
     3061 cccgtttttc agacggcatc agcctttccc tgcgcctgaa actcctgacc ggactgtggg
     3121 tcgggttggc ggcattgtct gtcgttttga cactgctgct ctctttgcgt ctggaaaacg
     3181 cggcctccgt catcgaagag gcgggcaact tgagaatgca ggcataccgt ctggcataca
     3241 tggcgggtga aggctcgccc cgtgcgcaaa ttgacaatca ggttgccgaa tttgaaaaaa
     3301 gtttaaaacg cattgcccaa agcgatgcca tccatccgct gattccttcg gacacccctc
     3361 ttgcttatga tttgatacaa tccatgctga ttatagattg gcaggcacac atcctccccc
     3421 cgctccagtc ctaccggcga ccgactcagg tcgatctcta ccgctttgcc ggaaacatcg
     3481 aactgttttt gcaggcattg gaaaatgcca acgaaaaaaa cacatggtgg ctcaggcgtt
     3541 ttcaatgggc aattatgttg atgacgctgg tgtcgtctgt actgatgctg ttttggcacc
     3601 agatttgggt tatccggccg ctgcaggcgt taagggaagg tgcggaacgc atcggacgga
     3661 ggtgtttcga tattccggtt cccgaaggcg gtacgccgga attcaaacag gtcgggcgtt
     3721 gtttcaatca aatgggcggc aggttgaaaa ttttatatga tgatttggaa ggacaagtcg
     3781 ccgagcagac acgcagtctc gaaaaacaaa atcaaaacct gaccctgctg taccaaacta
     3841 cacgggacct gcaccaatcc tacataccgc aacaggctgc agaacatttt ctaaaccgta
     3901 tcctgcccgc cgtaggagca gattccggca gagtttgttt ggacggcgga tccgatgttt
     3961 atgtttccat tcatcatgcg gattgcggca cagcagcttc ggatttgggg aagtaccatg
     4021 aggaaatctt ccccattgag taccagaacg aaacattggg caggctgttg ctcagctttc
     4081 caaacggcat ttctcttgat gaagacgacc gcatcctgct tcaaacacta ggcaggcaat
     4141 tgggcgtatc gcttgccggc gcaaaacagg aggaagaaaa acgcctgctt gcagtattgc
     4201 aggaacgcaa cctgattgcg caaggattac atgacagcat cgcacaagca ttaacgttcc
     4261 taaacctaca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
     4321 cagaaaacat cagctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
     4381 tgctgctcaa cttccgtacc aaaatcagca ataaagaatt tcccgaagcc gttgccgacc
     4441 tattcgcccg ctttacgcaa caaaccggga taacggtcga aaccgcctgg gaaaacggtt
     4501 cgttcctgcc gcctcaggaa gcgcagctcc aaatgatttt tatcctgcag gaaagcctgt
     4561 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaacacggcg
     4621 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
     4681 aacccacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccatg
     4741 ccgttttaga aatccgttcc caagctcaac agggaaccac cgtctcattg acggttgcat
     4801 ctgaagaaag cttgaaatga ctattaaaat tattctgata gacgaccata ccctcttccg
     4861 cagcggcatt aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
     4921 agacggcctc tcgggtatca aaatgatcag tcggctgcaa cccgatgtcg tcctgcttga
     4981 ccttgatatg cccggtatga acggacgcga agcactctcc caaatcatca gcatcaatcc
     5041 gcagcaggca gtgattatgc tgaccgtttc cgaagacagc gacgatttga ccgaatgtat
     5101 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgactttc tgctcgaaag
     5161 catacgcaaa gccgctgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
     5221 caaaagcctg atttcccccc aacctgccca agggacgcag gcactctcct cacttacccc
     5281 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
     5341 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
     5401 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
     5461 tgtgccggaa taggcgttca gacggcatat taggggtttt aatccccgta cggtcattcg
     5521 gataacagac caagcatgta agtttatgcc cccataagta cgcttggcat agcagtaata
     5581 ttgttcggtt tagtgttttc cgtttgcccc tatctgatac tgcaatatca gctatgccgt
     5641 ctgaaaacgc atcatcatga tattttcaga cggcataata aaaagcggaa atactaatgc
     5701 agggtaaaat gttccattcc gaatcccata aatatacaat ggcttatatc gtttaagcat
     5761 gtgtaaccca ccctcatatc aatcaatata tagtggatta acaaaaaccg gtacggcgtt
     5821 gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     5881 tta
//
LOCUS       DAWISC010000132         5853 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWISC010000132 DAWISC010000000
VERSION     DAWISC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.132"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            352..5799
                     /gene="iga"
                     /locus_tag="WIW87_001631"
     CDS             352..5799
                     /gene="iga"
                     /locus_tag="WIW87_001631"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ5174764.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGNGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHASTITLTGKSLITDPKTISIHYIQNNDDDDAGYYYYRPRKP
                     IPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQK
                     VEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQS
                     QPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELL
                     AKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQAL
                     AARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAEL
                     ARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDY
                     MAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEN
                     VSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHE
                     KVETVSLQPRAAQPRAQAATQLQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGAS
                     LTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQI
                     GGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKA
                     NFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLT
                     AGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLY
                     RNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1980 a         1407 c         1304 g         1162 t
ORIGIN      
        1 ttaccacgaa agcgggaatc taggactcag ggttaagaaa acctacatcc cgtcattccc
       61 tcaaaaacag aaaaccaaaa tcagaaacct aaaatcccgt cattcccgca aaagcgggaa
      121 tccaaaaaca aaaagccgca gaaatttaac ggaagaaccg aaccccgaaa atcggcttcc
      181 cgccttcgct tgaataacag aacaaaggaa gcggaaataa aaacatcggg attctttttt
      241 taaattggca ataaaacacc aaaatgaatg atttacacaa caaaaaatag tcaacaccac
      301 caaaccggcg taaaatacaa aacattatca cccttaaaac ggtaaaacct tatgaaaacc
      361 aaacgtttta aaattaacgc catatcctta tccatctttc ttgcctatgc ccttacgcca
      421 tactcagaag cggcattggt cagagacgat gtcgattatc aaatattccg tgactttgca
      481 gaaaacaaag gcaaattttt tgtcggcgca accgatttat cagtgaaaaa caaacaaggt
      541 caaaacatcg gcaacgcatt atccaacgta ccgatgattg attttagcgt tgcagatgtc
      601 aacagacgta cattaaccgt catcgatccc cagtatgccg tcagcgtcaa acatgtaaaa
      661 ggagacgaaa tctcttatta cgggcatcac aatgggcact tagatgttag caatgatgaa
      721 aacgaatatc gttcagttgc acaaaatgac tacgaaccaa ataaaaattg gcatcacggt
      781 aatcaaggtc gtcttgaaga ctacaacatg gcacgcctta ataaattcgt aacagaagtc
      841 gcacctattg caccaaccag tgcaggcggc ggcgttgaaa cctataaaga taaaaaccgt
      901 ttttctgagt ttgtgcgagt tggagcaggt acgcaatttg aatataacag ccgctacaat
      961 atgacagagc tttcacgagc ttatcgttat gccattgcag gtacgcctta tcaagacgtg
     1021 aatgtcacat cgaatctaaa tcaagaaggc ttgattggct tcggtgataa ttcaaaacat
     1081 cattccccag aaaaactcaa agaagtttta tctcaaaacg cattgactaa ctatgctgtg
     1141 ttaggcgata gcggttcacc attatttgct tatgataaac aagaaaaacg ctgggtcttt
     1201 ttaggtgctt acgattattg ggcaggctat caaaaaaact cttggcaaga atggaatatc
     1261 tataaaaaag aatttgcaga tgaaatcaaa caacgcgata acgccggcac catcaaaggt
     1321 aatggagaac atcattggaa aaccacgggc acaaacagcc atatcggttc gacagcggta
     1381 aggcttgcca acaatgaaag ggatgcaaac aacggacaga atgttacctt tgaaaacaac
     1441 ggcactttgg tattggatca aaacatcaac caaggcgcgg gcggcctgtt tttcaaaggc
     1501 gattacacag tcaaaggtat aaataatgac atcacttggc taggtgcggg gattgatgtt
     1561 gccgacggca aaaaagtcgt ttggcaagtc aaaaatccga atggcgacag attggcaaaa
     1621 atcggcaaag gcacattaga aataaacggc acaggcgtta accaagggca attaaaagtc
     1681 ggcgacggta cggttattct gaatcaacaa gccgatgccg acaaaaaagt ccaggctttc
     1741 tcccaagtcg gcattgtcag cggacgcggt acattggtat taaatagttc aaatcagatt
     1801 aatcccgata acctatattt cggtttccgt ggcggtcgtt tggatgccaa tggcaatgac
     1861 ttgacttttg aacacatccg caacgtggat gaaggcgcgc gcattgtcaa ccacaacaca
     1921 ggccacgcct ccacaatcac gctaacgggt aaatctttga ttaccgatcc caaaaccatc
     1981 tctattcatt atattcaaaa taatgatgat gacgatgctg gttattacta ttaccgccct
     2041 aggaaaccga ttccacaagg caaagatctt tatttcaaaa actaccgtta ttacgcccta
     2101 aaatccggcg gcagcgtgaa cgcaccgatg cccgaaaacg gacaaacgga aaataacgac
     2161 tggattttaa tgggcagcac acaagaagaa gccaagaaaa acgcgatgaa ccacaaaaac
     2221 aatcagcgta ttagcggttt tagcggtttc tttggcgaag aaaacggaaa aggacataac
     2281 ggtgcattaa accttaattt caacggcaaa agcgcgcaaa accgtttctt gttaacaggc
     2341 ggcactaatt taaacggaaa aataagcgta actcaaggca atgtcttgtt atcaggtcgt
     2401 ccaacaccac acgcaagaga ttttgtgaac aaatcttcag cccgaaaaga cgcacatttc
     2461 tccaaaaaca atgaagtcgt atttgaagac gactggataa accgcacatt caaagccaca
     2521 gaaattgcgg ttaatcaatc cgcatcattc tcttccggca gaaatgtatc cgacatcacc
     2581 gccaacatca ccgcaacaga caacgccaaa gtaaatttgg gttacaaaaa cggcgatgag
     2641 gtttgcgtgc gctcggacta taccggttac gttacctgca acacaggcaa cttatccgat
     2701 aaggctttaa acagctttgg agcgacacag attaacggca atgtgaatct gaaccagaat
     2761 gcggcattgg tcttgggcaa ggctgcatta tgggggcaaa ttcaagggca aggaaacagc
     2821 cgtgtcagcc taaaccaaca tagcaaatgg catttgaccg gcgacagcca agtacacaat
     2881 ctgtcattgg cggatagcca tattcatttg aacaatgcct ccgatgcgca aagtgcaaat
     2941 aaataccaca cgctcaaaat caatcattta tccggtaacg ggcattttca ttatctgacg
     3001 cacctggcga aaaatcttgg ggataaagtg cttgtgaagg aatccgcatc cggtcattat
     3061 cagctccatg ttcaagataa aacaggcgaa cccaatcagg aagggctgaa tctctttgat
     3121 gcatcatccg tacaagaccg ctcccgcctt tctgtttcct tggcgaataa ccatgtagat
     3181 ttaggggcat tgcgttatac aatcaaaaca gaaaacggta ttacccgatt gtacaatcct
     3241 tatgccgaga accgccgccg agtcaaaccg gccccgtctc ctgccacaaa cacggcttct
     3301 caagcacaaa aggcaacaca aacggacggt gcacaaattg ccaagcctca aaatatcgtc
     3361 gtcgcaccgc ctagcccgca ggcaaatcaa gccgaagaag caaaacgcca gcaagcaaaa
     3421 gcggagcaag tgaagcgtca gcaagcagaa gcagagcgta aatcggcaga gttagcaaaa
     3481 caaaaagcag aagccgaacg cgaagcaaga gagctggcga cccgccaaaa agcagaacaa
     3541 gaacgcagca gcgccgaact tgcacgtcga catgaaaaag aacgcgaagc ggcagagttg
     3601 tcggcaaaac aaaaagtcga agccgaacgt gaggctcaag cattggcggt acgccgaaaa
     3661 gcagaagccg aagaggcaaa acgccaagct gcagagcttg cacgccggca tgaaaaagaa
     3721 cgcgaagcgg cagagttgtc ggcaaaacaa agggtcgggg aagaggaacg ccgtcaaaca
     3781 gcccaatccc agccgcaacg ccgcaaacgc cgtgccgcac cgcaggatta tatggcagct
     3841 tcccaagacc gtcctaaacg ccgcggacac cgttcggtac agcaaaacaa tgtggaaatt
     3901 gcccaagccc aagcagaact tgcgcgccga cagcaggaag aacgcaaagc ggcagagttg
     3961 ttggcaaaac aaagggcaga agccgaacgt gaagctcaag cattggctgc gcgccgaaaa
     4021 gcagaagccg aagaggcaaa acgccaagct gccgaacttg cacatcgcca ggaagcagaa
     4081 cgcaaagcgg cagaattgtc ggcaaaccaa aaagccgcag ctgaagccca agcattggcg
     4141 gcacgccaac aaaaagccct tgcgcgtcaa caggaagaag cacgaaaagc tgcagagtta
     4201 gccgtcaaac aaaaagcgga aaccgaacgc aaaaccgccg aacttgccaa gcaaagggca
     4261 gcagccgaag cagcaaaacg ccagcaagaa gctcgccaga ccgccgaact tgcacgtcgt
     4321 caagaagcgg aacgccaagc agcagagttg tcggcaaaac aaaaagccga aacagatcga
     4381 gaagcggcag aatcggcgaa acgaaaagct gaggaagaag agcaccgtca agcagcccaa
     4441 tcccagccgc aacgccgaaa acgccgtgcc gcacctcagg attatatggc agcttcccaa
     4501 aatcgtccga aacgtcgcgg acgcagatct actctgccgg caccgccctc gccatcattt
     4561 gattcgtcag cttacgcagc acccagggcc ttgcataatc cggactggta tgaaaatgac
     4621 tatgaagaaa tccccttgga tgcgttggaa gatgaaaatg tatctgaatc agttgacaca
     4681 tcagacaaac agcctcaaga caatacggaa cttcatgaaa aatatgaaaa tgattatgaa
     4741 gaaatcccct tggatgcgtt ggaagatgaa gatgtatccg aatcggttga cacatcagac
     4801 aaacagcctc aagacaatac ggaacttcat gaaaaagttg agacagtgag tttgcaacca
     4861 agagccgcgc agccgcgagc ccaagccgcc actcagctgc aagcccaagc cgccgcgcaa
     4921 gccgatgcag tcagcaccaa taccaactcg gctttatctg acgcaatggc aagcacgcaa
     4981 tctatcttgt tggatacagg tgcttcatta acacggcaca ttgcacaaaa atcacgcgct
     5041 gatgccgaaa aaaacagtgt ttggatgtcg aacaccggtt atggtcgtga ttatgcttcc
     5101 gcacaatatc gccggtttag ttcgaaacgc acgcaaacac aaatcggcat tgaccgcagc
     5161 ttgtccgaaa atatgcagat aggcggcgta ttgacttact ctgacagtca gcatactttt
     5221 gatcaggcga gcggcaaaaa tacttttgtg caagccaacc tttatggtaa gtattattta
     5281 aatgatgctt ggtatgtggc cggcgatatt ggtgcaggca gcttgagaag ccggttacaa
     5341 acgcagcaaa aagcaaactt taaccgagca agcatccaaa ccggccttac tttgggcaat
     5401 acgctgaaaa tcaatcaatt cgagattgtc cccagtgcgg ggatccgtta cagccgcctg
     5461 tcatctgcag attacaagtt gggtaacgac agtgttaaag taagttctat gtcagtgaaa
     5521 acactaacgg ccggactgga ttttgcttat cggtttaaag tcggcaacct taccgtaaaa
     5581 cccttgttat ctgcagctta ctttgccaat tatggcaaag gcggcgtgaa tgtgggcggt
     5641 aattccttcg tctataaagc agataatcaa cagcaatatt cagcaggtgc cgcgttactg
     5701 taccgtaatg ttacattaaa cgtaaatggc agtattacaa aaggaaaaca attggaaaaa
     5761 caaaaatccg gacaaattaa aatacagatt cgtttctaaa atactaaatt catagcaaaa
     5821 taaaatgccg tctgaactca ggcttcagac ggc
//
LOCUS       DAWISC010000133         5838 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWISC010000133 DAWISC010000000
VERSION     DAWISC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.133"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            16..207
                     /locus_tag="WIW87_001632"
     CDS             16..207
                     /locus_tag="WIW87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174765.1"
                     /translation="MFQQGTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGIKSIS
                     DISRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="WIW87_001633"
     CDS             285..1232
                     /locus_tag="WIW87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ5174766.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="WIW87_001634"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="WIW87_001634"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ5174767.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPNDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="WIW87_001635"
     CDS             2508..3311
                     /locus_tag="WIW87_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ5174768.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHAQQSQLDTAYAYYKDDEKDKALAAIDRFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYKKLDKPRLAADTRRVLETNFPKSPFLKQPWRSDDMPWWRYWH"
     gene            complement(3539..5758)
                     /locus_tag="WIW87_001636"
     CDS             complement(3539..5758)
                     /locus_tag="WIW87_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ5174769.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTASAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFRAVRNIPA
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1390 a         1858 c         1407 g         1183 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagggg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgccata aacagacggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac atctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt atttatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagt cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatgaccta tctggcgcgt ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggc gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctgaccat cgaagtcggt atgcaaaact
     1081 cgggcctggc cgccgcgctt gccgccgcac actttgccgc cgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt tatcataatg ccgtctgaac
     1261 ggggcaaaca aaaccggcgg tttctgcccg gtcccttcag acggcctttg tttgaaatcg
     1321 gcggggacga ttgcccggct gctccgattc ggctgcctgc gttcgccccg cctttcaagc
     1381 cgcctcactc ccttacataa accacttcga catcatagtc gtcttcgttc caatcatcgt
     1441 cgtcgtccgc gccgaatttg tcctgccatt cttcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggacg gataacaaat gatatatgtc gtttggaatc ggtgcttcaa
     1561 acgaaacggt ttcgccgctt tccggatggg tgaaactcaa gcggtaggcg tgcaacgcct
     1621 gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcggcg gcaagcggat ggttggcctc gcgcatatgg acgcggattt
     1741 ggtgcgtcct gcccgtttcg agcgagcatt cgatatagct gtgggtaaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcgaagggg acgatgccgt
     1921 tggcgacggc gcggtagatg cgtttgaccg tgcgctcttg aagctgcctc acgagggaat
     1981 tttgcgccgg cagggttttg gcaaccacca tcagcccgct ggtttccttg tccaaacggt
     2041 gtacgatgcc cgcgcgcggt acttggctca attcgggaca gtgcgccaac aggccgttga
     2101 gcagcgtccc cgtccagttg cccgccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 cgacgatgac ggtatcgtct tcgtaaacaa tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaatac cgcatccaac cgcccgcctg
     2401 caagctcgag cggaacggtc agaccgatac ggttttcagt ttcggaagct gacgcaaagt
     2461 ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt acggtagata
     2581 aagatgccca gattacccaa gactggagtg tggaaaaact ctatgccgaa gcccaggacg
     2641 agctgaacag cagcaattat acgcgagctg tcaaattata cgaaatcttg gaatcgcgct
     2701 tccctaccag ccgccatgcc cagcaatccc aactggatac ggcatacgcc tactataaag
     2761 atgatgagaa ggataaggcg ctggccgcca ttgaccgctt ccgccgcctt caccctcaac
     2821 acccgaatat ggactacgcg ctatacctgc gcggtttggt actgttcaac gaagatcaat
     2881 ctttcttgaa caagctggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtatca ggcctttgcg gaactcgtcc aacgcttccc gaacagcaaa tacgccgccg
     3001 atgcgaccgc acgcatggtc aaactggtcg atgctttggg cggcaatgaa atgtcggtag
     3061 cgcgttacta catgaaacgc ggcgcatata tcgccgccgc caaccgcgcc caaaaaatta
     3121 tcggcagcta tcaaaataca cgctatgtcg aagaatcgct cgccatattg gaacttgcct
     3181 acaagaagtt ggacaagccc cggcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgttctta aagcagccgt ggcgatctga cgatatgccc tggtggcgtt
     3301 actggcactg attccgcacc gatgtccgga tgccgtctga agccctttca gacggcattt
     3361 ccccttctgg cgcaaacctt tccgttcatt caaacggttc tccctgccgt taaccgcaac
     3421 gccctttctg tttttgacgc acactgaaaa tcaaacgata tgccttgcca gcacgaaccg
     3481 gcgttaacgc caaaatgccg tctgaatccg tttcagacgg cattttatgt ttcaaacctc
     3541 actcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga gccttcacgc
     3601 cgccgcgtcc caagccgaat tgcagcgcac ccgacaaatc ggtacgcagc agtttaatgc
     3661 cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg ttggcataac
     3721 cgcttgaagc aacggcatat tcgggcgaaa cggcattgag gaacacgccc gacgaggacg
     3781 tattgctgcc gtgatgcccc aacaccaaca cctggctgta caggttgcct ccatacttgc
     3841 cgaccaggct ttcctcgccc ttcgtatcca aatcgcccgt taccagcagt gccgcaccgc
     3901 ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg cgttcagacg
     3961 gcctcaaaaa ctcgaaatct acgccgtccc attgccaacg ctgttccgca caatgccgcg
     4021 ccccctcata aaattccggc tgcccggcat aaatcccgcc ggcgggaata ttcctcaccg
     4081 cccgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacag aaccagcttg tccaaacggc
     4141 ggacacccgc cgcattcaaa ctcggcacaa tccccgtctg tgccgccgat gcagttccag
     4201 tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc tgccccgcat
     4261 cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa aacacaaacc
     4321 ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc agcaacagca
     4381 aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggcggcaacg gcaaactcgg
     4441 gcgacacatc ggcaagccac accaaaaacc gcaaagtata ttccgccaaa aatgccccca
     4501 actgttgcaa aggcgcaaac ggcacgaccg aacccagcaa cgccagcggc gtcaataccc
     4561 aagaaaacca cggaatcgcc accgcattga ccaaagggct gattaaaggc agcgaagcaa
     4621 acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg cgcacggcgg
     4681 tttgccgttt cccttcgtgc aaacgccccg aacacgccca tatcagggcc gccaccaaac
     4741 cgaaagacag ccaagtcccc acacccaaga ccgccaaagg gtcgaacagc agcactgccg
     4801 ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa gcccacgcaa
     4861 acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca agcagcgcgt
     4921 aaaacagcgc gcctgcacac cccgccgcca aaacccacac gcgcggccgg gcaggaaggc
     4981 gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa catcaccgcc accatcgtaa
     5041 cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggtcggaac gcctgccaca
     5101 attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc ccgatgccgt
     5161 ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg ctgcgccaaa
     5221 ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg accgtccccg
     5281 cgccgcctat cccgttggat aatgcccacg cctcacggtt caaaccgcgc aggttcaatt
     5341 cgccgacgac ggggtgcaca cgtgccgtta tccgccatct gctcccgacc gcccattcgc
     5401 gccgtttgta gtccgacagc agcaaatcaa acgtccgacc accgctgtcc acagcctttg
     5461 ccgcaaactg cacgcgccgc ccgtccgacc tcggcatatc cgccacttcc accgtcaacg
     5521 gcacacctga aaccgcctcc gcccgccatt gcgaagacag tgccgcttcc gttctgaata
     5581 cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca aaacgccttg
     5641 caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc acgaccggca
     5701 gcgcaaacga agccgccgcg ccgacaaccc aacacggcaa aaaccaacga cggaacacgt
     5761 ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga aaaaccgtca
     5821 taacaagatt tgaaaatg
//
LOCUS       DAWISC010000134         5693 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWISC010000134 DAWISC010000000
VERSION     DAWISC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.134"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            98..814
                     /locus_tag="WIW87_001637"
     CDS             98..814
                     /locus_tag="WIW87_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ5174770.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WIW87_001638"
     CDS             963..1865
                     /locus_tag="WIW87_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ5174771.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKCFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="WIW87_001639"
     CDS             1908..2681
                     /locus_tag="WIW87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ5174772.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEVGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WIW87_001640"
     CDS             2793..4481
                     /locus_tag="WIW87_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ5174773.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDCVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WIW87_001641"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WIW87_001641"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ5174774.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFLGAKNDDDHAWFKSADKVQTALEAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFIAPTLVTTRSADVRTFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1326 a         1539 c         1579 g         1249 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaat acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc acatatcatg cggcaataaa ggagagcagt
      961 agatggacag cctgatgacg ttgctttcgg tattgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtc ttggtgtatg
     1081 ctgtgctgct gctgatcggc gtctcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcattcg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagtgtttt ccagatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgccc cgtttctgat ggtggtgttt tccgctttgg
     1861 gttgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggtcgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtcaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcatcatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ttgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac attcccaaac cgcttcagac ggcatacgga atcattccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccatc atcgaagaag acctcgaaag cggcaaacat acagctgtcc
     2881 aaacccgttt cccgcccgaa cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagat gtcgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttgatt aaagacggca aagcgtatgt cgatgatttg acgcccgaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcgggtaa aaacagccct taccgcgacc
     3241 gcagcgtcga agaaaacctc gacctgttca cacgcatgaa aaacggcgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatcggg caacatcaat atgcgcgacc
     3361 ccgtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgtatct
     3421 acccgatgta cgactacacg cattgcatct ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaattcgaa gcacaccgtc cgctgtacga ctgtgtgttg gacaacattc
     3541 ctgcgccgca tgccacccgt ccgcgccaat acgagttttc ccgtttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtgacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcccaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt aacgctgaaa gactcttggc agcctaagta aaaccccatc cctgccgtct
     4501 gaatattgtt cgggcggaat ttctccttta cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 taaagacacc acctacgcga tgatgcgcga aatggcaaaa cgcggctggc ggctgtttca
     4681 cacattgagc ggggaattgt ctgtaaacgg cggtttggta acggcgcagg catcggcatt
     4741 tgaatttttg ggtgcaaaaa acgatgatga tcatgcgtgg ttcaaatctg ccgacaaagt
     4801 tcagacggca ttggaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaggtctt
     4921 taacagcgga caggcgatgc gcgactttaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttatcgcg cccacgctgg taacgacccg ttccgccgat gtccgcacat ttttgaaaga
     5041 acacggcgac atcatcatca aaccgctcga cggcatgggc ggtatgggaa ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcagc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttgggcggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg atgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tctgaaaggc ttttgcttca caaccgcctg gtttgattgg
     5581 gcaggcaggg ttttctctca gccggcaggg atacgcttaa taccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcg
//
LOCUS       DAWISC010000135         5551 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWISC010000135 DAWISC010000000
VERSION     DAWISC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.135"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(43..810)
                     /locus_tag="WIW87_001642"
     CDS             complement(43..810)
                     /locus_tag="WIW87_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5174775.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WIW87_001643"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WIW87_001643"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ5174776.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="WIW87_001644"
     CDS             complement(1777..2442)
                     /locus_tag="WIW87_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ5174777.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2621..4597
                     /locus_tag="WIW87_001645"
     CDS             2621..4597
                     /locus_tag="WIW87_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ5174778.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHKISASPQHTAAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNISKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4665..5357
                     /locus_tag="WIW87_001646"
     CDS             4665..5357
                     /locus_tag="WIW87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ5174779.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1450 a         1785 c         1200 g         1116 t
ORIGIN      
        1 tccacaaaag cgaatcatac tgccacattc cgacaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact cttatcgtca attttttcat
      181 agacacgatg cgccacaggc aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 gatggcggat tggaacagcg cggctttgaa cggcaaaagc cccaatccgg aggaagtccg
      361 cgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaaac
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcaaccgc cgtcaatacc
      721 gcgcaggttt ttcccggttt tgaacagcca gaccgccaaa cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gagcaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttcagtccta
      901 tttctgacag ttcgggcaat aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac cgggggcacg gctgattgtg ccgcccgtac actgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa atccctcaag gtactgccgc ccgtttcaat
     1081 ggcgcgctgc aacaccgctt tgacggtttc aaccaaaagc gcgcactctt tctttttcag
     1141 gcggttggca ggacggtggg gcgaaatgcc cgctctgaac aggctctcgt tggcataaat
     1201 gttgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc cttgcataca gataatccgc acaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc aacagcggat gatgttcttc gattccctca taccaaagta tcgcgccgaa
     1381 ctttctcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca gcacgcccgt ttgaaagcgg ataagcaggt atttcgccct
     1561 cctgccgcag gacaacacct gccggccgga caaaatctcc cccaaatcgg gattaatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattca ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaaccc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1861 tgcccttttg accaaaacca aaccgtacac tgcggcacac tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaaccg gcggcagata
     1981 caccataaat ttcccaaatt caatatcgaa accgacatcc gcaagccgtc ttttcaactc
     2041 gggcagcggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaag cgtccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaagg aacaccgcct tcaagcgtat gcggaaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatccagata ccgcccgaag aaatcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2521 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg aatataaatc atgtccaaac tcaaaaccat
     2641 cgctctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatacgccc aaaacacctc
     2701 atcacaccaa atcggtttgg cgattatgcg cttaaactct tcaatactcg acctgccccc
     2761 gacaaaacaa tatttccaat ccggcagcct gtggggcgag ctgcgccaag gcttccggat
     2821 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccacag
     2881 ctatttcaac agggtcatca accggagtag accctatatg taccatatcg ccaacgaagt
     2941 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3001 caccaaagcc aaatcacacg tcggcgcatc aggattatgg cagtttatgc ccgctaccgg
     3061 caggcattac ggcctggaaa aaacaccggt ttacgacggc aggcacgacg tttacgccgc
     3121 caccgatgcc gcactcaact atctgcaata cctctatgga ctgttcggcg actggccgct
     3181 tgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccatca accgcgcccg
     3241 cgcccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3301 tgtccccaag ctgctcgccg tgcgcaacat tattgccact ccccaatctt tcggcatgaa
     3361 tatcagcgac atagacaaca aaccctattt tcaggcagtc gaaccggatc gtccgctcga
     3421 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctaaaccc
     3481 cgcattcaac gtccccgcgt ttatccccaa aagcaaacgc aaactgctgc ttcctgtcgc
     3541 gtccgtacaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3601 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3661 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3721 cagcatcctt gtcgccaaga acggcaaaac ccttcagacg gcatcggaat ccgtcgtttc
     3781 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3841 cgtcggcatt gcccgaatcc gacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3901 tttgccgcag aaaaccgtcc gtacggaacc cgatcccctt gtccgtattg ccgaacctgc
     3961 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag accgccatgc cgtctgaaac
     4021 ccaaaccgca acactcgcgc agatcatccc ccaaaacgac atgcaggcgg cagacgaact
     4081 catgcagctt gttgcccgaa acaacctgcg ccgacaggct gaagaaacca tctccgccgt
     4141 catcggcacg cctgacacag ttgccgaaca caaaatttcc gcatctccgc aacataccgc
     4201 tgccgccgac ggcaaacgcc gggtacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4261 cgaagccgaa atctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4321 gttcaacatt tccaaacgct acaacgtcag tgtagccgac ctgattgtcg ccaacaacat
     4381 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4441 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4501 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4561 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4621 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa cttatcaaat
     4681 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt gccggcggtt
     4741 atgttttcat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatccg
     4801 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4861 tcatgctcaa caagcctgaa gattacgaaa cttcgcacaa acccaagcac taccgcagcg
     4921 tattcagcct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     4981 atgcagatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacagcctga
     5041 cttcgccgag cagaaaaatt cccaagctgt acgaagtaac gctcaaacac cccacaggag
     5101 aaacgctctg cgaaaccttg aaaaacggcg tgctgctcca cgacgaaaac gaaaccgttt
     5161 gtgccgccga tgccgttttg aaaaacccga ccaccctgct gctgaccatt accgaaggaa
     5221 aataccacca agtcaaacgc atgatcgccg ccgccggcaa ccgcgtgcaa caccttcatc
     5281 gccggcgatt cgcacatctg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5341 aatgtccaaa attctgaaat aacatccaag aattccattt atattcatcc acatactcat
     5401 taaatatatg gtttaaccca atttaacgca aaaaatcaat ttacgatata atccatttgt
     5461 ctgagtaacg cctgttcaag caggcttatg agtaagacgt tttccccgta atgtgttttg
     5521 ccatctatac ttctcccctt gtatagatgg t
//
LOCUS       DAWISC010000136         5547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWISC010000136 DAWISC010000000
VERSION     DAWISC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.136"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            52..1455
                     /locus_tag="WIW87_001647"
     CDS             52..1455
                     /locus_tag="WIW87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ5174780.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKSAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            1577..2293
                     /gene="trmB"
                     /locus_tag="WIW87_001648"
     CDS             1577..2293
                     /gene="trmB"
                     /locus_tag="WIW87_001648"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ5174781.1"
                     /translation="MTDTAENQTQNNWQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(2923..3285)
                     /locus_tag="WIW87_001649"
     CDS             complement(2923..3285)
                     /locus_tag="WIW87_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174782.1"
                     /translation="MSQLDFNLELFIKNSNFDDYKIFDIVKEAIETQDEIGSILRTHL
                     FFERILEAWIYAKCNKEDFFNDTQIGFRTKLKIARNLGLFSEVYDIGERLNKIRNTIA
                     HHVLREVNQENLTLYMKV"
     gene            complement(3398..5425)
                     /gene="uvrB"
                     /locus_tag="WIW87_001650"
     CDS             complement(3398..5425)
                     /gene="uvrB"
                     /locus_tag="WIW87_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ5174783.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIEK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEQGIVPQQIKKQVKDIIDGVYHEEDGSKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRDIKENLLFGAE"
BASE COUNT         1395 a         1415 c         1383 g         1354 t
ORIGIN      
        1 ctaacttcat aacgaataac gatgattcga caaaacggaa aacgatctga catgaacaat
       61 cccgacttac cctatcggca ggccttagaa tgcctgtctc aaaaacaata taactttacc
      121 gaagtccgcc gactgctgac agaagcgttc tcggcaggtc atcccgccgc cgcattcgag
      181 ttggcaaaac acctgatgga cgcggacagc ccctaccaag accgcgaaca aggtatggaa
      241 atgctccgca tcgccgctga acagggacat ccctacgcgc gttacaatct ggcatatatc
      301 caagaattgg aaggcgcacc cccggaaacc ctgataccgc tttacagacc gttggcagaa
      361 gaaggactgc ccgaagcgca agtccgcctg atgtaccttc tgtacgcgtc ccgacatttt
      421 gaagaagcct tggaatgggc aaaaacaagc gcaaaaaaca acaaccccca cgggcaatac
      481 ctgcttgccc aatactgccg gtacggcaca ccgccggatt ttgaaacggc gcacctgctc
      541 taccgaaaat cggcggcaca aggcttgccg gaggcacatt ggcagctcgg gctgcaatat
      601 cgtttcgggc aagggacgaa agtcgacacg gcacaggccg tcaatcattt gcgcgccgcc
      661 gcacaacaag gatacattcc tgcctacacc ccacttgccg agctcatcct acctacggct
      721 cctgatgaag ccgttcactg gtttcaacag gccgcacagg aaaatgaccc cgatgcccat
      781 gccgcacttg ccgacatcta cctgcaaggc aagcatctgg aaagaaacca caaacttgcc
      841 ctgcatcatg ccgaagcagc cgccgccgaa cgccatcccg aaggtttgcg gatactgggc
      901 gacatctgcc gctacggttt gggcatagcc cccgatacgg aaaaagcccg gcattattat
      961 cggcaggcag ccgaagccgg cagcctttcc gcctatcaga aactcatatc cgacagcgcg
     1021 ttaaaccatc ctgaccaata cggcggcatt aaagattccg ccatcaggcg gcaaagggca
     1081 gaacggcttt atcaaaaagc ccaagccctg cattacggat tacaatgcgc gcccgaatac
     1141 gcagccgcgc tcaaactcta cacagaagcc gcagaactcg gacacagcaa agcccaaacc
     1201 aatctgggca gcatgtatta cttcggacag ggtatgaccg ccgactacaa tgaagcacgc
     1261 aaatggtttg aaaaagccgc cgcgaaaaaa gacagtatgg cgttctacaa cctcgcctgc
     1321 atccattaca gcggacacgg cgtcgagccg gacaaagaaa aagcctgccg ctacctgcaa
     1381 gaagccataa acaacggata cgggcaaaaa agcgtcctgc aagaactgct gcaacaatgg
     1441 caaaatgccg tctgaacagc gttacaccta ccctgccgaa acgaaacagg tataatcgcc
     1501 cctttccctt cccgccgtcc gaacaggcat ttcacattca gacggcatcc tgattgcaca
     1561 agcgtacgaa agcattatga cagacaccgc cgagaaccaa acacaaaaca actggcaagc
     1621 cggacacccc cgcagcatcc gcagcttcgt cctccgccaa agccatatga ccgccgcgca
     1681 gcaacgcgcc atcgatacct tatgggacag cttcggcatc gactaccaag caacaccggc
     1741 cgatcttgat gcccgtttcg gaagcagccg acccaaaatc ctcgaaatag gcttcggtat
     1801 ggggacggca accgcagaaa tcgcccgccg cctgcccgaa accgacttcc tcgccatcga
     1861 cgtacacggt cccggcgtag gcaacctgct caaactcata gacgaaaacc atttagaaaa
     1921 catccgcgtg atgcggcacg atgccgtaga agttgtcgaa aatatgctgc aagacggctc
     1981 gctcgacggc atccacatat tcttccccga cccgtggcac aaaaaacgcc accacaaacg
     2041 ccgtctgata caagccccct tcatcgccaa actactgccc aaactcaaaa ccggcggcta
     2101 tatccacctg gcgacagact gggaagaata tgcacagcag atgcttgaag tcctcagtag
     2161 cttcgacagc ctgcaaaata cggcggcaga ctacgccccc accccggact accgccccga
     2221 aaccaaattc gaagcgcgcg gcaaacgcct cggacacggc gtttgggact tggtattcaa
     2281 acggatcgga taacaaacca ctgtttgaaa atgccgtctg aaacatgttt gcttacagac
     2341 ggcatttttt caagataaag cagcaagtga tgtttcgata caaggtttaa aacaatggtt
     2401 tgaacggcaa aaacgcctgc gtaccgcacg catccttagt aggttttatg cacatcggtt
     2461 ttaaagtttg tgccgcccgc aggtagtatg tgatagctac gcacgcggtt ggtgtgatgt
     2521 aggctacggc ttgctggtta caaccgtaaa aaagtaagtg ccgccattgc ggtaaaaacg
     2581 aagggatttc atagtgttat gctcgtaatg attttgtaga ttggattctc gaatccgacc
     2641 ttttgggcat tgctgcaatg gattgcaacg acgggaatgt tgaaggtttt gtcggataca
     2701 agtatccgac ctacgcttgt tgctatatat cttttgattt aatgactaaa tatgacaaag
     2761 ttaaagtgca gattacagca agcatgatat gcttctttag gcttttatca ttccatgata
     2821 tagatatttc ttccttttca ttttctttat aaaattttaa acctatatca ccatttttcc
     2881 attcctggtg gtttactatg attttatttt taaaagaatc tcttaaactt tcatgtaaag
     2941 agttaaattt tcttgattta cttcccttag tacatggtga gcaattgtat ttctaatttt
     3001 atttaatctc tcccctatat catatacttc gctaaataag ccaagattac gcgcaatttt
     3061 tagttttgtg cgaaatccaa tttgtgtatc attgaaaaaa tcttctttat tacattttgc
     3121 atatatccat gcctctaaaa ttctttcaaa aaataaatgt gttcgtaaga ttgaacctat
     3181 ttcatcctgt gtttcaatag cttctttcac gatatcaaaa atcttataat catcaaaatt
     3241 tgaattttta ataaataatt ctaaattaaa atctaattga gacataataa gtgcccattt
     3301 caaaaataaa tctatattct agttaatata atagttattc taatatctaa attaaataat
     3361 aaactactat ttttatatcc acgacaaagt ctaagtctca ctccgcccca aacaacaaat
     3421 tctctttaat atccctaatc ctatcccgca acacagccgc ctcttcaaac tgcaaatccc
     3481 tagccgcctg ctgcatggct ttttcgagtt tggcgatttc tttaatcgca tcttcttcgt
     3541 tgtgaatctc gcccacttta accttgtttt tacctttcag acggccttta ctgccgtctt
     3601 cttcgtggta cacgccgtcg atgatgtctt tgacctgttt tttaatctgc tgcggcacga
     3661 tgccctgttc ttcgttgaat ttaatctgtt tttcacggcg gcgttcggtt tcgtcgatag
     3721 cggctttcat ggagtcggta attttgtcgg cgtacaggat ggcgacgccg ttcacgttgc
     3781 gcgcggcgcg gcctatggtt tgaatcaggc tgcggtggga gcgcaggaag ccttctttat
     3841 ctgcgtcgag gatggcgacg agagagactt cggggatgtc gaggccttcg cgtaagaggt
     3901 tgatgccgac gagtacgtca aacaggccga gccgtaaatc tctaatgatt tcaacgcgct
     3961 cgacggtgtc gatgtcgctg tgcaggtagc gcactttgat accgagttcg ctgtaatagt
     4021 cggtgagttg ctccgccatg cgtttggtga gggtagtaac gagtacgcgt tcgccttttt
     4081 caatgcggtc gttgatttcg ctcattaagt cgtcgacttg ggtggcaacg gggcggatga
     4141 tgatttgggg atcaaccagc cctgtggggc ggacgacttg ttcgaccact tgtccggcgt
     4201 gttcttcttc gtatttggcg ggggtagcgg aaacgaagat ggtttgcggc atgacttttt
     4261 caaattcgtg gaatttgagc gggcggttgt cgcgggctga aggcaggcgg aagccgtagt
     4321 cgacgaggtt ttgcttgcgc gatgcgtcgc ctttgtacat gccgccgatt tgggttacgg
     4381 taacgtggct ttcgtcgatg aacatgatgg cgttgtcggg caggtagtcc atcagcgtag
     4441 gcggcggttc gccttctttt ttgccggaaa agtggcggga gtagttttcg attcctttgc
     4501 agaagcccat ttcgtagagc atttcgaggt cgaaacgggt gcgctgttcg atgcgttgtt
     4561 gttcgacggg gcgttgttcg cgggcgaaaa attcgatgcg ttcgcgtaat tcttctttga
     4621 tggactcgca ggcgcgcaag acggtgtcgc gcggggtaac gtagtggctg gacgggaaga
     4681 cggtgtagcg gccgacgcgc tggataaggc tgcctgaaag cgggtcgaac atatcgaggc
     4741 ggtcgatttc gtcatcaaac aggctgatgc gtaaggcgtt ttcggagctt tcggcggggt
     4801 acacgtcaat cacgtcgccg cgcacgcgga agctgccgcg tttgaagtcc aaatcgccgc
     4861 gttcgtattg catggaaacg agcgtggcga tgatgtcgcg ctgctcgatg gtatcgcctt
     4921 ctttgacgga caacaccatt tgttgatact cggtcgggtc gccgataccg taaatggcgg
     4981 acacggtggc gacgataatc acgtcgttgc gcgtcattag gtttttggtg gcggaaaggc
     5041 gcatctgctc gatgtgttcg ttgatcgcgc tgtctttttc gatgaacaaa tcgcggctgg
     5101 gcacataggc ttcgggctgg taatagtcgt agtaggagac gaaatattcc actgcgtttt
     5161 cggggaaaaa ttcgcgcatt tcggcgtaaa gctgggcggc aagggttttg ttgtgcgcca
     5221 tgatgatggc ggggcggccg ctttgggcga tgacgttcgc catggtgtag gttttgcccg
     5281 aaccggttac gccgagcagg gtttgatagg caaggccgtc tgaaagccct tcgagcaggc
     5341 ctgcaatggc ggtgggctgg tcgcctgcgg gcgggaaggg ttggtggagt ttgaaggggg
     5401 aatttgggta ttggataact tccataatct tgcctgtgat gcgtttgcgg acaaagcgtg
     5461 cagtagggat gggtcggaaa cgtctttcag acggcataag gcggtgaaat cctgaatgta
     5521 tgccgtctga aacccaatcg ctaccca
//
LOCUS       DAWISC010000137         5487 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWISC010000137 DAWISC010000000
VERSION     DAWISC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.137"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            71..496
                     /locus_tag="WIW87_001651"
                     /pseudo
     CDS             71..496
                     /locus_tag="WIW87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            502..1731
                     /locus_tag="WIW87_001652"
                     /pseudo
     CDS             502..1731
                     /locus_tag="WIW87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1789..2409
                     /locus_tag="WIW87_001653"
     CDS             1789..2409
                     /locus_tag="WIW87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174784.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLIGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2420..2551
                     /locus_tag="WIW87_001654"
                     /pseudo
     CDS             2420..2551
                     /locus_tag="WIW87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2548..3042
                     /locus_tag="WIW87_001655"
     CDS             2548..3042
                     /locus_tag="WIW87_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ5174785.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3130..4791)
                     /locus_tag="WIW87_001656"
     CDS             complement(3130..4791)
                     /locus_tag="WIW87_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ5174786.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4921..5463)
                     /locus_tag="WIW87_001657"
     CDS             complement(4921..5463)
                     /locus_tag="WIW87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ5174787.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1309 a         1487 c         1398 g         1293 t
ORIGIN      
        1 ctatattatc ccgtatgcgg attggtttta agatttgtaa atttgatttg catcaaaaaa
       61 tcgccgatag atgattcata taatatcaat attaaagagt atcggtatat cggggatagt
      121 catgtcctgt ttttcaatca aacgtatgtc cgcgtttcgg gcgcggataa cggcgttttt
      181 tgccgccttt gtctttttga cggcggcact gcccgcttat gcggagcgtc tgcctgattt
      241 tctggcgaaa atacagcctt cggaaatttt tccgggtgcg gaccgttacg gcaagccgga
      301 aggtaagcct atggttgccc gcgtttacaa aggcgatgag cagttgggct tggtctatat
      361 cacgaccgat gcggtcaata cgcgcggtta ttcgagcaaa ccgattgata cgctgatggt
      421 gttggcaaac gacggcacga tagccggggc gaaactggtc gaccatcacg aaccgattat
      481 gctgatcggt atcccgcatt tgcccgcgcc cgggcgggcg atacgctcaa actggcttcc
      541 ggcgtatata aaaccaaact tcacattgac aaaccgatta cgattgaagg gcctgccgac
      601 cgttccgcaa ccatcgaagg cgacaggagc gggcgtacca tagccgtaca cgcgccggac
      661 gtaacgctcc gcaacctgac cgttacccgt tccggtatga gcctgcccgc aatggatgcc
      721 ggtatttatc tcgaagaaac tgccccgcgc gccctgattg aacacaacaa tattttggat
      781 aattcggtcg gcgtatatct gcatggttct gccgatgcga tggtgcgcga gaataaaatc
      841 gtcggcgacg cgactttgcg cgtgaacgag cgcggcaacg gcgttaccgt ttggaacgca
      901 cccggtgcgc aggtcgtcgg caacgacatt tccaaagggc gggacggcat tttttccaat
      961 accagcacgc acaacaccta caaaaacaac cgcttcagcg atttgcgttt cgccgtccac
     1021 tatatgtaca ccaacgacag cgaaatcagc ggcaatattt ccgtgggcaa caatatgggc
     1081 tatgtgctga tgttttccga gcggctcaaa gtattcgaca atatcgccgt cggcagccgc
     1141 gatcagggca ttatgctcaa ctatgtcaac tattccgata ttcacgacaa cattatcaac
     1201 aaggcaggca agtgcgtatt tgcctataat gccaactacg ataaactttt cgccaatcat
     1261 tttgaaaact gtcaaatcgg catacacttt accgccgcca tcgaaggcac gtccttgcat
     1321 gacaattcct ttatcaacaa cgaaagccag gtcaaatacg tcagcacgcg ctttctcgat
     1381 tggagcgagg gcggacacgg caactattgg agcgacaaca gcgcgttcga tttgaacggc
     1441 gacggcttcg gagacagcgc gtaccgcccc aacggcatca tcgaccaaat catctggcgc
     1501 gcgcccgtat cgcgcctttt gatgaacagt cccgcaatca gcatcgtcaa atgggcgcag
     1561 gcgcagtttc ccgccgttct gcctggcggc gtggtggaca gcaaaccgct gatgaagcct
     1621 tatgccccca aaattcaaac ccgttatcag gcgatgaagg acgagctact caaagaagtc
     1681 gaaacgcggc agtcggaatg gggcagggcg gaaaacggtt ctttgaacta gtctgcttca
     1741 gacggcatcc ggattcaaat gccgtctgaa aacacaaaag gaacaaccat gaccacacat
     1801 catgtcgaat tgaggaaggt aaccaaacgg ttcggggcgc aaaaagccgt caaccaagtc
     1861 gatttggttt tgaaggcagg agaaagcgtc gggcttgccg gacacaacgg cgcgggcaag
     1921 tccaccatta tgaagctgat actcgggctg attaccccga ccgaaggcga agtgatgctt
     1981 ttgggcgaac gtaccggtag caaagcgggg gcgcggcttc gcagccaaat cggctacctg
     2041 cccgaaaccg ttgcgctgca cccttcgctg atcggcatcg aaacgctgga tttttatgcc
     2101 aaacttaaaa aacagccgct cacgcagaac cgggggctgc ttgagcgcgt cggcatttca
     2161 caggcggcac accgccgcgt cggcacttat tctaaaggga tgcgccaacg ccttgccttg
     2221 gcacaagccc tgctgggcga gcccaaagtc ctgctgtttg acgaaccgac aaccggtctt
     2281 gaccctgcat cacgacaaat gttttacgaa gtcgtgcgcg aactcaacgg gcgcggcgcg
     2341 accgtattgc tcagcaccca cgcccttgcc gagttggacg ggcacgccga ccgcattatc
     2401 gtggattaaa tttaatccac tatatgcggg tatggcgggt ttgagcggac aaatcagcct
     2461 gaccgtcccc gttttgctga ccgctcaggt tttatgggtt atcattccgc ttgttttggc
     2521 agccggaatt tttagaaagc gacaaatatg aaaaaaaccc tgttggcaat tgttgccgtt
     2581 tccgccttaa gtgcctgccg gcaggcggaa gagggaccgc cgcctttacc ccggcagatt
     2641 agcgaccgtt cggtcggaca ctattgcagt atgaacctga ccgaacacaa cggccccaaa
     2701 gcccagattt tcttgaacgg caaacccgat cagcccgttt ggttctccac catcaagcag
     2761 atgttcggct ataccaagct gcccgaagag cctaaaggca tccgcgtgat ttacgttacc
     2821 gatatgggca atgttaccga ttggacgaat cccaatgccg acacggagtg gatggatgcg
     2881 aaaaaagcct tttacgtcat cgacagcggc tttatcggcg gtatgggtgc ggaagacgcg
     2941 ctgccgttcg gcaacaaaga gcaggctgag aaatttgcaa aggataaagg cggtaaggtt
     3001 gtcggtttcg acgatatgcc tgatacctat attttcaaat aatattgaaa aaaccggccg
     3061 aaactttaaa aacgagtttc ggtcggtttt tcttttcttg ttcggtatag tgtcggcagg
     3121 aaagaacctt cacatcccgc cgtaattcgg cccgctcgcg ccttcggggc aaatccaagt
     3181 gatgttttgc gtcgggtctt tgatgtcgca ggttttgcag tgcacgcagt ttgccgcgtt
     3241 gatttgcagg cgcggattgc cgttttcttc aacaatttcg tacacgccgg ccggacaata
     3301 gcgcgtttcg ggcgaggcgt attctttgta gttcacgtct atcatcgttt gcggattgtt
     3361 cagcaccaaa tggtcgggct ggttttcttc gtgcgcgaga ttggcaagga agacgctgct
     3421 caagcggtcg aaggtcaaca cgccgtcggg tttcggataa tcaatcggct tacacgcggc
     3481 ggctttttta agctgctcgt tgtctttgcc gtgatgtttc aaggtccacg gggctttgcc
     3541 tctgaaaatc atctgatcga tgccggtgta gattgagccg aggtaaacgc cccatttgaa
     3601 tgacggacgg acattgcgcg cggcgtaaag ctcttgatac agccagcttt gttcaaaacg
     3661 ttgctgataa tccgccgcct ctttgccgct gtcgaaaccc tccacttctt caaggttttc
     3721 caacaagggg aacacggctt cggcggcgag catggcggat ttcatcgcgg tatgaatgcc
     3781 tttgatgcgc ggcatattga ggaaacccgc cgcatcgccg accaaaatgc cgcctttgaa
     3841 cgagagcttc ggcaaacttt gcaaaccgcc ttcaatcagc gaacgcgcgc cgtaagcaat
     3901 gcggcggccg ccttcaaagg ttttgcggat ttcgggatgg gttttgaaac gttggaactc
     3961 ttcaaacggc gacagataag gattttgata gtccaaaccg accacgaagc cgacggcgac
     4021 tttgttgtcg tcgaaatggt aaacaaacgc gccgccgtag gttttgctgt ccagcggcca
     4081 gcctgcgctg tgcaccacca aaccgggctg atgctgttcg gacggcactt cccaaacttc
     4141 tttaatgccc aagccgtaag tttgcggctg gctgttttgg tcgagttgga aacgttcgat
     4201 gatttgtttg gaaagcgaac cgcgacaacc ttcggcaaac agggtttgct gcgcccaaag
     4261 ctccatgccg ggttggaatg aatcggtcgg ctcgccgtct ttgccaatgc ccatattgcc
     4321 ggttgcaatg cctttgaccg aaccgtcttc gtgatacagc acttcggcgg cggcaaagcc
     4381 cggatagatt tccacgccca tattttccgc ctgctccgcc aaccagcgca cgacttcgcc
     4441 caagctgacg atgtagttgc cgtgattgtc gaaattcggg gtaatcggca ggttgaacgc
     4501 ttttttctcg gtcaggaaca acactttgtc ctgcgttact gtgcgtgtca gcggtgcgcc
     4561 tttttctttc cagtcggaaa tcaactcatt cagcgcaatc ggatcgataa ctgcgccagc
     4621 cagcgaatgc gcccccacct ccgaaccttt ctccaccacg caaacgctga tttcgcgccc
     4681 gttttgttcg gcaagctgct tgagtttgat ggcggcagac aaacccgacg ggcctgcgcc
     4741 gacaatcacg acatcgtatt gcatactgtc gcgggtgatg gattctgtca tggcggttcc
     4801 tgtgtattta ttattgaatt gcaaatccgt aattatacaa cgggaacata tagttaccaa
     4861 atacaacaaa ggtcgtctga aaaccatatt ttcggttttc agacgacctt tgtcgaaatt
     4921 tcaataagca cgccaccatt ttacctgtcc gaccgcaaac tccgtctgac gtttcggact
     4981 gcgtgtgaaa aacgccttat ccccgccggc atccctccct ttcggcacaa ccgccaaaat
     5041 cttacctgcc aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat
     5101 tgaaacatga cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa
     5161 taccgagaaa tgatcatcct tgcggtattt cagatacacg atgacgggga tttgcaactg
     5221 tgcaagctgc tcgaaagaca gggcatagcc tttcgcttca aaacccaaat caggcataat
     5281 gcgccgcata tcctcaaacg acgcgggcat ctgctcctta tccagttttt ttaacacgtc
     5341 ctcttccgtc agcttttgcc cgtaaaaatt gttcaaaagc gtcaccaccg aagccgcccc
     5401 gcaggaaaaa tccaaatcct gctttacaat attgaaatcg cgcctttctt tccaactctg
     5461 cactttgatt tttccataag caacagg
//
LOCUS       DAWISC010000138         5437 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWISC010000138 DAWISC010000000
VERSION     DAWISC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.138"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(86..286)
                     /locus_tag="WIW87_001658"
     CDS             complement(86..286)
                     /locus_tag="WIW87_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174788.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            436..1575
                     /locus_tag="WIW87_001659"
     CDS             436..1575
                     /locus_tag="WIW87_001659"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ5174789.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAIVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGFGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1572..2153
                     /gene="metW"
                     /locus_tag="WIW87_001660"
     CDS             1572..2153
                     /gene="metW"
                     /locus_tag="WIW87_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ5174790.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2270..3106
                     /locus_tag="WIW87_001661"
     CDS             2270..3106
                     /locus_tag="WIW87_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ5174791.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPSKDDKTKEVGASAASSSASSAP
                     SQTDLQPTASAPDNVKQAESAPPSNCTSLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQNALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYISQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNTG
                     RI"
     gene            complement(3157..3717)
                     /gene="efp"
                     /locus_tag="WIW87_001662"
     CDS             complement(3157..3717)
                     /gene="efp"
                     /locus_tag="WIW87_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ5174792.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3760..4908)
                     /gene="earP"
                     /locus_tag="WIW87_001663"
     CDS             complement(3760..4908)
                     /gene="earP"
                     /locus_tag="WIW87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ5174793.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDQLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSAPEKLAAFVSKHQKIR"
     gene            complement(4927..5205)
                     /locus_tag="WIW87_001664"
     CDS             complement(4927..5205)
                     /locus_tag="WIW87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174794.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         1380 a         1401 c         1420 g         1236 t
ORIGIN      
        1 ctataaagca cctcctaccg caagcccttt tcatcaaata tgccgtctga aacagaaaaa
       61 tgcgtttcag acggcatttt ttccgtcaat acgaatccgg aatgtgtttg aaatacagca
      121 taaacagata ccaaaacagc atcgcggcgg ctgcaccgag cagtacccat gcgctgattt
      181 cggcgacttc gcgcagggcg cgtttttgcc tgcggctgaa aaggcggttg ataatcaacg
      241 cggcaagtgc gaaaaagaaa aaaatacgga aaagtctgcc tatcatgtcg ggaagcggtg
      301 gaggtcgtct gaaaaagtat gattcgtgtg cggcggatga gggtcaaacg cgtccggctg
      361 cttgtccgac cgtgtcaaaa agtcatgccg tttgcgatat aataacgctt ttcgccgcat
      421 ttgttggaac aggatatgag tcaaaatgcc tcggtgggca ttgtaatgcc ccaaaaaatt
      481 ccgtttgaga tgccgctggt tttggaaaac ggtaaaactt tgccgcgttt cgatctgatg
      541 attgaaacct acggcgagct gaatgctgaa aaaaacaatg cggttttaat ctgccacgcg
      601 ctgtcgggca accatcatgt tgcgggcagg cattcggcgg aagataaata tacgggctgg
      661 tgggacaata tggtcggacc tggcaaaccg attgatacgg aacgtttttt cgtggtcggt
      721 ttgaataatc tgggcggctg cgacggcagc agcgggcctt tgtcgattaa tcctgaaacg
      781 ggcagggaat acggcgcgga ttttccggtg gttacggtga aggactgggt aaaatcccaa
      841 gccgcgcttg ccgactatct cggtatcgaa caatgggcgg cgattgtcgg tggcagcttg
      901 ggcggtatgc aggccttgca gtggacgatt tcctatcccg agcgcgtgcg ccatgcttta
      961 gtgattgcgt ctgcgccgaa actgtccacg caaaatatcg cgtttaacga tgtggcgcgt
     1021 caggcaattt tgaccgaccc tgatttcaat gaaggacatt accgcagcca caataccgtt
     1081 cctgcacggg gcttgcggat tgcccgcatg atgggacaca tcacttatct tgccgaagac
     1141 ggtttaggca aaaaattcgg acgcgatttg cgttccaacg gttatcaata cggctttggt
     1201 gttgaatttg aagtggaatc ctatcttcgc tatcagggcg acaaattcgt cggacggttc
     1261 gacgccaaca cttacctgct catgaccaag gctttggact atttcgatcc ggcggcagat
     1321 ttcggcgaca gcctgacccg cgccctgcaa aatgtgaaag cgaaattctt tgtcgccagc
     1381 ttcagcaccg actggcgttt cgcacccgaa cgttcgcacg aactggtcaa ggcactgatt
     1441 gccgcccaaa aatccgtgca gtatatcgaa gtcaaatccg cacacgggca cgatgccttt
     1501 ttaatggaag acgaagccta tatgcgtgcg gtcgccgcct atatgaacaa cgtttataag
     1561 gaatgtcagc aatgaacctg cgcgatgatt tgcaactgat ttacgaccgg atacccgaag
     1621 gcagccgcgt cttggacttg ggctgcggcg acggcgaact gcttgccgca ttggtcgaac
     1681 acaaaaaatg cagcggctac ggcatcgaaa tcgacacaaa tagcgtcatc gccgcgatgt
     1741 cgcgcggcgt aaatgttatc caagccgatt tggaagaagg tttaaccgca ttcaacgatc
     1801 aaagttttga tgtgattgtg ttgagccaaa ccatccaagc gatgcagaat accgaaaaaa
     1861 tcctgcgctg cctgatgcgc gttgccaaac aggcgattgt cagcttcccg aatttcggct
     1921 actggcgcaa ccgcgtccaa atcgcactcg gcggacatat gccggtttcc gaacgtatgc
     1981 cctaccattg gtacgacacg cccaatatcc attggtgcac gctcaaagac tttgatttat
     2041 tgtgcgccaa aaacaacatc cgcatcctcg agcgcgccgt gatgacgggc aacagacagg
     2101 tcaaacattt ccccaacctt ttgggcagcc tggcgtttta ccgcgtcggg taagacaggt
     2161 tgagaacctt gtcggcatta acaggttctt cggttctatt aacaacaggc cgtctgaaaa
     2221 atgaaacggt aggttttcag acggcctctt taatactgga aacaatagaa tgaaaaacaa
     2281 aacctcatca cttctcttat ggcttaccgc aatcatgctg accgcgtgtt ctccgagcaa
     2341 agacgataaa accaaagaag tcggtgcatc cgctgcttcg tcctccgcgt catcagctcc
     2401 ttcccaaacc gatttgcaac cgaccgcatc cgcccctgat aacgtcaagc aggcagaaag
     2461 cgcgccgccg tcaaattgca ccagcctgca ccccgccacc ggcattgacg atctcatgca
     2521 gcaaatcgcc gaacacattg actcggactg tctgtttgcc ctttcccatc acgaactgga
     2581 aacccgtttc ggcttacccg gcggtggcta tgacaacata cagcggctgc tgtttcccga
     2641 catccgccct gaagatcccg actaccatca gaaaatcata ctggcaattg aagacttgcg
     2701 ttacggaaag cgcacgatca gccggcaggc acaaaatgcc ttgatggaac aggaacgccg
     2761 cctccgagaa gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg gacaaggcga
     2821 ggagccgaaa cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt cgagccggca
     2881 caacaacgga cttggcggca atttccaata catcagccaa ttgcccggct atctgaaaat
     2941 acacggagaa atgcttgaaa accaatcact cttccggctg tccaaccgtg aacgcaatcc
     3001 cgacaaaccg tttttagaca tccattttga cgaaaatggc aaaatcacgc gtattgtcgt
     3061 ttacgaaaaa aacatctact tcaatccaaa cacggggcga atataaaaaa aatgccgtct
     3121 gaaacgtttt cagacggcat ttttcaatgc ggcaaatcag gcgcgtttgc ggaattcgcc
     3181 ggtgcgggtg tcgatttcga ctttatcgcc gttttcgatg taagacatca cttggatttc
     3241 ggtgccaccg accaggcggg cggttttcat cactttgccg gaagtatcgc ctttgacggc
     3301 aggctcggtg tactcgactt cgcgcacgat gatggtgggc agttctacgg agatagggtt
     3361 gccttcgtag aaggttactt cgcattggtc ttccataccg tcaacgatga atttcaacgc
     3421 gtcgccgatg ttgtcagctt cgatttcgta ttggttgaat tcttcgtcca taaagacgta
     3481 catcgggtcg gcaaagtagc tgtacgtaca gtttttgcgg gacaggatga ccacgtcgaa
     3541 tttgtcgtcg gctttgtaaa tggtttcgga agccgcgccg gtcagcaggt ttttcagttt
     3601 catgctgact ttggcggaag agcggccgcc tttgatgtat tcggtttttt gaacgaccat
     3661 aggatcgttg ccgaccataa atacattgcc ggcgcgcagt tcttgtgctg ttttcattgt
     3721 gattttccaa tcaaatcggt tgtcgtaaaa cgcgctattc tagcgtattt tttgatgctt
     3781 tgaaacaaag gcagcgagtt tttcaggagc tgacggctgc ccgaaaagat aacggctcca
     3841 atcctccgcg ccttgccgcc agccgttttg atgttgttgc agggtttgcc aacattcgag
     3901 gcgttgtgtt gcggataaag cctctccgcc gttgaggtcg tccgaaagac ggcggtgtgc
     3961 cgacacggtt tcgggcgtgt agaaaccgtg tgccttatcc caaaaggcgt ggagtttgtc
     4021 gagatggaca ttctcgtctt gcgggtagat gtgccaaaag aagggtttgc ccgcaagctg
     4081 ggcgcgcacg aaactgtctt cgccgcggat gacggcgcag tcggcaaggt gcagcagttg
     4141 gtcgaagtcc tgttgcggca cgaaagggat tttgacgagg cggacggatg ccgtctgaaa
     4201 aacatcgccg tcgttttgca gggcatcttg cggaataacg ccgctttgtt tgaggctgtc
     4261 gatgatttgc gtccccgcca gcaacagtgt catcgggctg cctgcctgtc gccacatttc
     4321 cagccacttt gcccaaacat cgctccgata gccgaaaagc agccattcgg aggcgttttt
     4381 ttcgggcagc atcagccgct ctcgcagggc ttcagtatcg aaacggacgg cttcgcagta
     4441 atcacgttcg cgtatcaacc cgccgctttt ttcgctgaaa cccataaacc aaaaatattt
     4501 ttgaacaccc tcctgcggcg aaggcatcag atgcagcctt tcattgcttt cctccgcgct
     4561 caaatattcc caattcagcc aaagcggctt gtgtcggcgg ataatgtgca gcacattttc
     4621 gggcaggtcg caggcaaaag tttcgatgac gacatcggga acaggcgcgg tatcaatatc
     4681 tgccgcatcg gaatgccaag tgcggacatg aatatcctga tgaacgcagg gaacatcggg
     4741 caaatcaggg caaagcgcac gcaaggcgga cacatcgtcc gtccacaaat gcacctgcca
     4801 accgagttcg cggtgcaaaa cacgggcgag ccgccacgaa acgccgatgt cgccgaaatt
     4861 gtcgatgacc ttgcaaaaaa tccaacagac aaaaggagga gtattcatag aaataacgga
     4921 aacgggctaa aacgcgccaa aatagtggaa caaatacaca atcagcaaaa taatcggaat
     4981 aaacagcaac atagtttgac gtttcaaatc aaactcttgt ttcaaatgat gcttgatggt
     5041 gtcgaaaagg acgaatttga acaaaaacgt cgcttcgcag gcaaggatga tgccgttaat
     5101 caaatgctgg ggtttgtctt cgggcggatt ggcgacgaaa acggcaagcg tcgccaaggg
     5161 gatgaggcag acgacgagcc agcggacaag caggtttcgg gacattgcaa ggctttcaaa
     5221 aaagcaaaac gccattatcg ccccaagcgc acgccttgtc taacggacgc accagagatg
     5281 ccgtctgaag ccttgccgca ggaaaacgcc gcaccgccga acctttaaat acgcccggga
     5341 cgggcattcc ccaaaaaatc ggacactgcc gctgatgcct gcggacaagg cggcatcggg
     5401 cgcgggcata ttgaaaaccc ggcgcgtcgg tttgaaa
//
LOCUS       DAWISC010000139         5403 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWISC010000139 DAWISC010000000
VERSION     DAWISC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.139"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(247..1506)
                     /gene="rho"
                     /locus_tag="WIW87_001665"
     CDS             complement(247..1506)
                     /gene="rho"
                     /locus_tag="WIW87_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ5174795.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WIW87_001666"
     CDS             complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WIW87_001666"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ5174796.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4522..5343
                     /locus_tag="WIW87_001667"
     CDS             4522..5343
                     /locus_tag="WIW87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ5174797.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1141 a         1266 c         1588 g         1408 t
ORIGIN      
        1 acaaaaatga gtgcggttcg gcgcaacctt gaatcaagtt cccgcatcgg ttttcattgc
       61 cggtacggat gcgttcaagc cggctttgca aaggccgcgc tttcggcaag cggacacgga
      121 cactgccgac ggttgcgccg ttaacggggg gagggaggag ctgcgccgac cgtgtgaatg
      181 aaagtgccgt ctgaaacccg attttcaggc ttcagacggc atttcgcatt aatgcgggcg
      241 gcgcgtttat ttgccgcgca tcagttcaaa gaaatcgtcg ttgtttttag aggctttgat
      301 tttcccgatt aaaaattcgg ctgcctcgat ttcgtccatc gggtgcagga acttgcgtaa
      361 gagccacata cgttgtaact ggtcgtttgg gacaagcagc tcttcgcggc gcgtgccgga
      421 tttgttgatg ttgatggcgg ggaagaggcg tttttccgcc atacggcggt caaggtgcaa
      481 ttccatattg ccggtgcctt tgaattcttc gtaaatcaca tcgtccatac ggctgccggt
      541 ttcaaccaat gcggtggcga tgatggtcag cgaaccgcct tcttccacgt tgcgcgccgc
      601 gccgaagaaa cgtttgggac gatgcagcgc gttggcatcg acaccgccgg tcaggatttt
      661 gcccgaggta ggcacgacgg tattgtaggc gcgggcaagg cgggtaatcg aatccagcag
      721 gatgaccacg tcttttttgt gttccaccat acgcttggct ttttcaagca ccatttcggc
      781 aacttggacg tggcgttgag ccggctcgtc aaaggtggag gagactactt cgccacggac
      841 ggagcggctc atttcggtta cttcttcggg acgttcgtca atcaagagga cgatgagttc
      901 gacttcggga tagtttgcgg taacggcgtg ggcaatgttt tgcagcatca cggttttacc
      961 gcttttgggc ggggcaacca agagggcgcg ctgacctttg ccgatagggg aaatcaggtc
     1021 gatggcacgt ccggtcaggt tttcttcgga ctttaagtcg cgttccagct tcaactgttc
     1081 ggtcggaaac agcggggtca ggttttcaaa caggatttta tggcggcata cttccgggtg
     1141 gtcgccgttg atggtatcaa gcctgaccag ggcaaaatag cgttcgttgt cttttgggac
     1201 gcgcacgctt ccttcgatgg tgtcgcccgt atgcaggttg aagcggcgga tttgggtggg
     1261 cgagacatag atgtcgtcgg ggccggcaag ataggacgtg tccgcgctgc ggaggaagcc
     1321 gaagccgtcg ggcaggattt caagcgtgcc ggagcaggtg aaaccctcgc cttttttcat
     1381 catctggcgg acgatggcaa atacgaggtc ttgtttgcgg aatcggttgg cgttttcgat
     1441 gccgtgttct tccgccaatt ctaagagttt ggaaatgtgc agggtttgta attcggagac
     1501 gtgcataata atgatgtatt ttgaagagga aaaagacagg cagatgccgt ctgaaagaag
     1561 aagctgaccg ttgccggttg ctcggggaag ggggaattgt aggcagtcgg cgcgtgggtg
     1621 tcaaatatta tcgcggacgg ggcatcggca ggaaatgccg tctgagcgga gctgcttgga
     1681 aaaaaatacc cccgcgcttt tcaggctcgg gggtatgggc attgattatt tgttcaattc
     1741 attcgccaaa tatagccaag tttcgatgac ggtatccggg ttcagggaaa cgctttcaat
     1801 gccttcctca accagccatt tggcgaagtc cggatggtcg gacgggcctt gaccgcagat
     1861 gccgacatat ttgttctgct tgcggcaggc ggagatggca aggtgcagca tcactttgac
     1921 ggcagggttg cgttcgtcaa acgattcgga taccaagccg ctgtcgcggt cgagaccgag
     1981 ggtcagttgg gtcatgtcgt tcgagccgat ggagaagccg tcgaagtatt gcaggaattg
     2041 ttccgccaat accgcgttgc tcggcagctc gcacatcata atcaggcgca ggccgttttt
     2101 gccgcgttcc aagccgtttt ctttcagggc tttgacaacg gcttcggctt cgcccaaagt
     2161 gcggacgaac ggaatcatga tttcaacgtt ggtcaacccc atttcatcgc ggacgcgttt
     2221 caaggctttg cattccaagg cgaaacagtc tttgaagttg tcggcgacat aacgcgccgc
     2281 accacggaag cccaacatcg ggttttcttc atgcggttcg tatacgttgc cgccgaccag
     2341 gttggcgtat tcgttggatt tgaagtcgga catacggacg atggttttac gcggataaac
     2401 cgatgcggcc aatgtcgcca cgccttcggc gattttatcg acgtagaagt cgacagggga
     2461 cgcgtaaccg gcgatacggc gggtaatttc cgcttttaat tcgtcgtctt gtttgtcaaa
     2521 ttccaacaag gctttggggt ggataccgat ttggcggttg atgataaatt ccatacgcgc
     2581 caagccgatg ccttcgctgg gcaggttggc gaagctgaat gcgagttcgg gattgccgac
     2641 gttcatcatg acttttacag gtgctttagg catattgtct aaggcgacat cggtaatctg
     2701 tacgtccaac agaccggcat agataaagcc ggtatcgcct tcggcacagg atacggtaac
     2761 ttcttgaccg tttttcagca attcggttgc attgccgcag ccgacaacgg caggaatgcc
     2821 caattcacgc gcgatgatgg cggcgtggca ggtacggccg ccgcggttgg taacgatggc
     2881 agaagcacgt ttcatcacgg gttcccaatc cggatcggtc atgtcggtaa cgagtacgtc
     2941 gccggcttcg acggaatcca tctcggaagc atctttaatc aggcgcacct tgccctgacc
     3001 gactttctga ccgatggcgc ggccttcgca taatacggtt ttgtcgccgt tgatggcgaa
     3061 gcggcgcagg ttgcggttgc cctcttcttg ggattttacg gtttcgggac gggcttgcag
     3121 gatgtagagt ttgccgtcca agccgtcgcg tccccattcg atatccatcg ggcggccgta
     3181 gtgtttttcg atggtcagtg cgtaatgcgc caactcagta atttcttcgt cggtaatgga
     3241 gaagcggttg cggtcttcct cggggacatc gacgttggtt acggatttac cggcttctgc
     3301 tttgtcggta aaaatcattt tgatgtgttt tgaacccatg gttttacgca ggatggcggg
     3361 cttgcccgct ttgagcgtgg gtttgaacac ataaaattcg tccgggttga ccgcaccttg
     3421 tacgacgttt tcgcccagac cgtaagagga ggtaacaaag acgacttgat cgtagccgga
     3481 ttcggtgtcg agggtgaaca tcacacctga tgcgccgctg tcggaacgca ccatgcgttg
     3541 aacgccggcg gaaagggcga cgatgtcgtg ttcgaagcct ttgtggacac ggtaagaaat
     3601 ggcacggtcg ttatacaggg aagcgaatac atggtgcatc gcttctttaa cgttatccaa
     3661 gccgttgatg ttcaagaagg tttcctgttg tccagcgaat gatgcgtccg gcaggtcttc
     3721 ggcagttgcg gaagaacgta cggcaacgga aatgtccgca ccgccggcat cggcaaccat
     3781 tttgttccat gccgcttcga tttcggcatc gagctgttcg gggaaaggcg tatccaaaat
     3841 ccattggcgg atttctttgc cgacgcgtgc cagttcggca acgtcttcga catccaattt
     3901 tgccagtgcg gcggaaatgc gttcgctcag accgttgtgt gcgaggaatg cgcggtaggc
     3961 ttcggccgtg gtggcaaagc cgccggggac gcgaacgcct ttttcggtca gctgactgat
     4021 catttcgccc agcgaggcgt ttttaccgcc cacgcgttca acatctgtca tacgcaggtt
     4081 ttcaaaccag attacgtagt tgtcggccat ttgtgtgtcc aatccaaaat atgttaaaaa
     4141 agaaacaaat ccgcgtgctt attttaagcg attcgttcct ctgctgtcat gtgttttatc
     4201 cgttttaaaa tcatgatgcc gtctgaaaaa ttgcggtttc ggcgtgtgta gcggtttgaa
     4261 acttacagcc ggtatacttc tttttttggg tattttcttt gtaaaacagg tggtttgaat
     4321 aggttaatgt tttttctgtt tgattttttt gtttattttt taaaattttc tgccaaaaaa
     4381 tactttatat aaataatttt atttcaaaat tatattgtgt ctgtttgggt gtaatccgag
     4441 gtaggtgtgc tgcgggtgct ttccttgtgt ctgctgctgc tgttatgatg ggattttaaa
     4501 cctgtgtttt aaggatggaa gatgagcagt ccgcgccatg tgttttacat ttccgaccgt
     4561 accggtctga ctgctgagaa tatcggcgag gcgttgctga accagtttgg caatctgtcg
     4621 ttcaaacgcc atacgcatcc gtttgtcgat acgccggaaa aggcgcgcgc ggtggtggag
     4681 aaggtcaatc ggagccggca ggaaaacggt cagcgtccga ttgcgtttgt cagtgtggtt
     4741 gatgacgaaa tccgtcggat tatcaaaggg gcggatgctt ttcagattaa tttctttgag
     4801 acttttttgg gactgttgga gaaggaactc aataccgaag ccacggcatc cgggcagggg
     4861 catcacagta tcggtaatac gaagcgttat gatgcgcgta tggaagcggt caatttttct
     4921 ttgaaccacg acgacggggt cagcgataag aaccttcagg aagcggatgt aatcttgatg
     4981 ggtgtatcgc gttcgggcaa aacgccgacc tgcctttacc tcgccctgca atacggcatc
     5041 cgtgcggcaa actatccgct gattcccgac gatttggaat cggccgatct gccgcgtatg
     5101 gtcaagcctt atagggataa gctgttcggg ttgaccatcc agccggaacg tttgcaggcc
     5161 atccgccaag agcgccgccc gaattcaact tatgccaaaa tcgatacatg ccgcagcgag
     5221 gtggcggacg cgcagagtat gttcagacgg catgggattc cgtttgcgaa tacgacggat
     5281 aagtcggttg aggaattggc ggtacacatc cttcaggcgt gcaagctcaa acgcaggttt
     5341 tgacgggctt tgattcggtt tgaaggcgga actgccgtct gaaatcaggt ttcagacggc
     5401 agt
//
LOCUS       DAWISC010000140         5330 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWISC010000140 DAWISC010000000
VERSION     DAWISC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.140"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            304..1737
                     /locus_tag="WIW87_001668"
     CDS             304..1737
                     /locus_tag="WIW87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ5174798.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1846..2634
                     /locus_tag="WIW87_001669"
     CDS             1846..2634
                     /locus_tag="WIW87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ5174799.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3002..4558
                     /locus_tag="WIW87_001670"
     CDS             3002..4558
                     /locus_tag="WIW87_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ5174800.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4575..5321
                     /gene="surE"
                     /locus_tag="WIW87_001671"
     CDS             4575..5321
                     /gene="surE"
                     /locus_tag="WIW87_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ5174801.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1244 a         1357 c         1520 g         1209 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaagcagctg cagcacaaaa attatgggcg
      481 gcaaaaactg cgttggaacg cgccgatgtg ttgtggcgtt ggtattttca gattaaagaa
      541 aacaaagaag cattagcgcg cctgatgacg atggagcagg gcaaaagcct gaccgaggcg
      601 cgtggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cccgtcggcg ttaccgctgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggccgcgc ctgctttggc ggtgggttgc gcgatgatcg tcaaacccgc atcgctcacg
      841 cctttgagtg cgtatgcctt ggcttcgttg gcttacgaag cgggcatacc gcaggatttg
      901 ttgcctgttg tcagcggcag tgcttcggaa atcggccatg aatttgctac gaacccgatt
      961 atccgcaaaa tcagcttcac cggctcgacc gaagtcggcg caaaaatttt tgccgacagc
     1021 gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggcgcgctcg ccagcaagtt ccgtaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgctcaat ccgccattta cgacgaattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggt
     1261 gtgaaccaag ggccgctgat tgaggaaaaa gcggtggaga aagtcgagca gcacatcgcc
     1321 gacgcgcttg ctaaaggtgc aagctgcctg accggcggca aacgcagcgc gttgggcgga
     1381 acgtttttcg aaccgactgt tttaagcggc gtaacggcgc aaatggcggt ggcacgcgaa
     1441 gaaaccttcg ggccgttgtg tccggtattc cgttttgaaa ccgaagccga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggca gcttaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgtagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctagaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcagga tttcagacgg cgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat ggaaaacgga
     1861 aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcacct cttaatcagc
     1921 ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc ttatccttat
     1981 gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga tattgtatgt
     2041 ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc acgcatggtc
     2101 gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct gcacagcgtt
     2161 tcgctggcgc gtcctgtggt ggaagcgttt gaacaggatc gtatgaccat tgttacggcg
     2221 gcggaagtcg tggtcgaaga tttgcacaaa gcccccgaag ggctgcaaag cctgtcgtgg
     2281 ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa gaacaagacg
     2341 ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccagtg gctgccgtat
     2401 tccgacaagg aagcagaaga aatccgcaaa catctgaaac cgctgaaagt cttggcggat
     2461 gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga aggggaggag
     2521 ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat taccgataaa
     2581 gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc ttaagcgttg
     2641 ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac tgtatttgag
     2701 aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg ccgtccgtgt
     2761 gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg gtatggcgtt
     2821 ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa ggtacacgca
     2881 catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg taccgtctgt
     2941 tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga atacgaaaat
     3001 catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca cgcttttggt
     3061 gttggaagtc gtattgggga tagacaatct tgtctttgtg gcgattttgg caaacaaggt
     3121 ccagcccgca cggcgcgacc gcgcacggat tatcgggctg gggctggcag tcgtcatccg
     3181 catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc tgttccaaat
     3241 cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta ttttcctgct
     3301 ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt ttaccgttgc
     3361 cgacagccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc tgatactgga
     3421 tgccgtgttt tccatcgatt cggtcattac tgcggtggcg atggtcgatc atatcgtcgt
     3481 ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca aactcttgac
     3541 cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt tgttgatgat
     3601 cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct acctctacgc
     3661 cgccatcggc ttctcgattt taatcgaatt gtttaaccag atttcgcagc gcaacagccg
     3721 caaaaacgac tacatcggca gctcgtggcg caagcgcacc gccgaaaacg tcttgggtat
     3781 gatgggtata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg acgacgcgca
     3841 ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg aacgcccgat
     3901 tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc aaagccgcga
     3961 agaacagtgt gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg tcggtaaggc
     4021 gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc aactgctgga
     4081 aacaggcggt ctcgacattc agacggcatt gcgccagccg ctcgtcctgc ccgacagcac
     4141 caccgcgctg ggcgcaatcg aactcttccg ccaaagcagc gcggattatg ctttggtggt
     4201 ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg aaaccatcgc
     4261 aggcgagttc cccgaagaat ttgagcgcga agaagaacca gccgttcagg ggaatcccga
     4321 tgaaagcctg acggtggaag gcgcgttgga atatgtggaa ctcgcaccgc aactcaacct
     4381 gccgcagcag gaggaagatg ccgatttcca tacggttgcc gggctgatta tggaagaatt
     4441 gcaaaccatc cccgatgtcg gcgattttgc cgatttccac ggctggcggt ttgaagtggt
     4501 cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg aagaataagc
     4561 attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc tccgaaggca
     4621 ttgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtc gcgcccgaac
     4681 gcgacaggag cggggtcagc aattcgctga cgctggaacg ccctttgcag ttgaaacagg
     4741 cgcaaaacgg gttctactat gtcaacggca cgccgaccga ctgcatccac atcgggcagt
     4801 ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg ggcgcgaata
     4861 tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc taccttatgg
     4921 gcatacccgc cgtggcgttt tccttaaacg acgcttccgg acgctattgg gcgaccgcag
     4981 aacaggcact gtggacattg ttggcgcatt ttttcaaaaa ccccccgcag tcccctattt
     5041 tgtggaacat caatatcccc gccgttgcgc cggaagatgt gcggggcatt aaaatcgccc
     5101 gtttgggcag gaggcatcac ggtcagaacg tcattcccgc gcgcaatccg cgcggcgaac
     5161 agatttattg gataggaccg gtcggcgaag tttccgatcg ggaagaggga acggatttcg
     5221 gtgaatgcgg cgcaggtttc attaccgtaa cgccgctgca aatcgacctg accgcctatc
     5281 cggacatggc ggaaacagcg gcgttctggc atgcggactg accgtttcat
//
LOCUS       DAWISC010000141         5324 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWISC010000141 DAWISC010000000
VERSION     DAWISC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.141"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WIW87_001672"
                     /gene_synonym="tbpA"
     CDS             complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WIW87_001672"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ5174802.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGGATPDAAQTQSLKEIT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDENNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGILFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2931..5144)
                     /gene="lbpB"
                     /locus_tag="WIW87_001673"
     CDS             complement(2931..5144)
                     /gene="lbpB"
                     /locus_tag="WIW87_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin-binding protein LbpB"
                     /protein_id="HEZ5174803.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGSSVETTPVNRPAVGAAMRLPRRNIASYKQDGTEIPDKHQAEEHLPLKEK
                     DILFLDGTLKEQADKLKKKINERYSDVRVITSKKEEEKYQYQFVRAGYVFTRAEGKDN
                     EKEKTSDGKEFVNRFSYDGFVYYSGERPSQSLPSAGTVQYSGNWQYMTDAKRHRTGKA
                     VSSTDLGYTTYYGNEIGATSYEARDADDREKHPAEYTVDFDNKTLNGKLIKNQYVQNK
                     SNPNEPKKPLTIYDITATLDGNRFTGSAKVSTEVKTQHADKEYLFFHTDADQRLEGGF
                     FGDNGEELAGRFISNDNSVFGVFAGKQKTETENAADTKPALSSGKHTKILDSLKISVD
                     EASDKNPREFAISSMPDFGHPDKLLVEGREIPLVNKEQTIELADGRKTTIRTCCDFLT
                     YVKIGRMQTERPAAKPKAQDEERDEEDTGVDSVEEGEDEIDDEEGTEDAAVKDEGSEE
                     DEAVEGEDEAEEPEEESPTEEGGSGSDGILPAPEAPKGRNIDLFLKGIRTAETDIPKT
                     GEAHYTGTWEARIGKPIQWDNQADKEAAKAVFTVDFGKKSISGTLTEENGVEPAFHIE
                     NGKIEGNGFYATARTRENGINLSGNGSTDPKTFQASNLRVEGGFYGPQAEELGGIIFN
                     NDGKSLGITEGTENKVDVEAEVDAEVDVGKQLESEVKHQFGVVFGAKKDMQEVEK"
BASE COUNT         1131 a         1442 c         1234 g         1517 t
ORIGIN      
        1 gaaatcttgt ttatattgaa tcaaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcgag
      121 cgcgagactg aagttcctgc ccggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cagtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctcaggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagggacag tcccggccgg ttggataccg atttcggttt
      541 gatgcggttg tacgccaatg tggtgtacaa accttcgggc agtttgccat acacgccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttgaagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaattgtcc tgcctgattc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tataacggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcccg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcacggta agacacggaa aaatgccggt tcggtttgaa
      961 caagatgccg ctgttccacg aacggtcaac ataccgcccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt cgtacctgcc gcccaagctg aaatcgaaat atttgccgat
     1081 tgaaaaacgg tcgttcaaag aaatatggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcagggttt cgatgtagcc gcagaccgac ccttcgacga cttcgggctt
     1201 acccaaaaga tacttatctt gattgttttc atcgaatccc gtggattccg aaatccttgc
     1261 cgcattgtgg gaaagctgtt cggggcggga aatcgctttg gaagcatcgt aaccgaagcc
     1321 caaagtcaga tggtgtttcg tccatttgtt tttcagcgat ttctcaaacg aggcattcaa
     1381 aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg tcgtaggaat acggtttgtc
     1441 cgccgacgcg cggcaggatt tgtccacagc aggatacacg gcgcaattca gcttcagcgt
     1501 gttgttatcg gttgccacgc cctgtttgtc aaacgacaac accgccttat ccgcccaatt
     1561 gtcagaatac gcttcgtttt cataacgata cagcaaaccc atacggcggc ggcggtgatg
     1621 ttcgtcaata aatttggtgc gggaatattt caaacctatg cccctgacca aatttttatc
     1681 gcccttccac tcttctatat tcggcacaaa atacaagccg tcgcggaaat cgtcgccgtc
     1741 gtacaccccg ctcttgtctc taaacttttc cgcctcgtcc gtaccgtaat actgtttttc
     1801 cgtcatatcg cggatatcgt aacgctgttt ggtatcctca aacacgccgc cgacataatg
     1861 cctgccgccg aagcggtagc ccagcttggc aagccaagag ccgctgcggt aatccatcgg
     1921 atcgggcaat atcctgccgc cgcccgtgta agcttgggcg gacagatttt cgtggcgcgc
     1981 ctgcgcctcc cgcacctgcg cctcttcttc agcacttaaa ggctgatttt gttcaatacg
     2041 ttcttttacc cagcggttga gctggttgtt caaatatttc ccgtagcccg ccaattttgc
     2101 cacgggcttg gattcacgct cgccctctac tgagaaaaat ggctctcttg tcttgcgttt
     2161 aatatcgtat gtctgacgga acgcgtccaa acggtctatg ccgtattcca ccccgtccgc
     2221 aatatcgccg tgcgggcgcg tttcccgccc ttggcgttcg gttcggatta acagcccttc
     2281 ccaaccgtct ttgctgaacc ccgcgccgag cgacttcata aattggcggt ttttactgcc
     2341 gtaggcggtt tttgcctgta tcccccaact tttgccgtct gaaatcaggt ctgccgcctc
     2401 tttggtgcgg aaggcgaccg cgccgccgag tgcgccgctg ccgtgatcgg acgaaccggc
     2461 acctttgtcg atttccaccg tgctgatgtt ttcatattcg atttcgttga ttgcaccgct
     2521 gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg gtaaacgcct gtatttgggc
     2581 aacaccgtcg accgaaaccg ccacacggtt tttatccacg ccgcgtatcg agtagccgcc
     2641 gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga tcgtagcgcg tcaggtcgcg
     2701 gataccgagt acctgttctt tgttcaacgt ttccgacgtt ttgacgattt tgcccaaacc
     2761 ggtcgcctct ttcgatcgcc gtcccacttt ggcggcacgg acggtaatct ctttcaggga
     2821 ttgggtctgc gcggcatcag gtgtcgcccc ccccgcttgg gcagcataag ccggaaaagc
     2881 ggttgcaatg gccaaggcag tcagagtcag cggaaaaccg tgtttcttat tcatttttcc
     2941 acctcctgca tatctttctt cgcaccgaat accacgccga attggtgttt aacttcagat
     3001 tctaactgtt tgccaacatc aacttcagca tcaacttcag cttcaacatc aactttattt
     3061 tcagtacctt cagttatacc aagagatttc ccatcattat tgaaaataat accgcccaat
     3121 tcctccgcct gcgggccgta aaatccccct tctacacgaa gattactagc ttggaaggtt
     3181 ttggggtcgg tcgaaccatt tcccgaaaga ttgatgccgt tctcccgagt gcgtgctgtc
     3241 gcgtagaaac cgttgccctc aatcttgccg ttttcaatat ggaaagcagg ttctacaccg
     3301 ttttcctccg tcagcgttcc ggaaatcgat ttcttgccga aatcaacggt aaatactgct
     3361 tttgccgctt ctttatccgc ctgattgtcc cattgaatgg gtttgccgat acgcgcttcc
     3421 caagtgccgg tatagtgtgc ttctccagtt ttcggaatat ccgtttccgc cgtgcggata
     3481 cctttcagga aaaggtcgat gttcctgcct ttaggggctt ccggagcggg caggatgccg
     3541 tctgaaccgc tgccgccttc ttctgtcggc gattcttctt cgggttcttc agcttcatct
     3601 tcaccttcta cggcttcgtc ttcttcgctg ccttcgtctt ttacggctgc gtcttcggtg
     3661 ccttcttcat cgtcgatttc gtcttcgcct tcttcgacgc tatcaacgcc tgtatcctct
     3721 tcgtccctct cttcgtcctg cgccttcggt ttggcggcgg gacgttcggt ttgcatccgt
     3781 ccgattttca cataggtcag aaaatcgcag caggttcgga ttgtcgtttt cctaccatcg
     3841 gcaagctcga tggtttgttc tttgtttacc aaaggaattt cacgcccttc gacaagaagt
     3901 ttgtcgggat gaccaaaatc gggcatagag gaaatggcaa actcacgggg atttttatca
     3961 cttgcctcgt caacggaaat tttcagagaa tccaagattt tggtgtgttt tccagacgac
     4021 agggcaggtt ttgtatctgc tgcgttttct gtctctgttt tttgtttgcc tgcgaatacg
     4081 ccgaatacgc tgttgtcgtt gctgataaac cgtccggcaa gctcttctcc gttatcgccg
     4141 aaaaaaccgc cctcaagccg ctgatcggca tcggtatgga aaaacaaata ttctttatca
     4201 gcgtgttgcg tcttcacctc ggtgctaact ttggcactgc cggtaaagcg gttgccgtcc
     4261 aatgttgcgg taatgtcgta aatggtcagc ggttttttgg gctcatttgg attactttta
     4321 ttttgcacat actgattttt aatcagcttg ccattcaggg ttttgttatc aaaatcaacc
     4381 gtatattcgg caggatgctt ttccctgtcg tcggcatccc tagcctcata agaagttgcc
     4441 ccaatttcat taccataata tgtggtataa cccaaatccg tactggaaac cgccttacct
     4501 gtccgatgac gtttggcatc ggtcatatat tgccagttac cggaatattg caccgttccc
     4561 gcgctcggta aagattggga aggacgttct ccggaataat atacaaaacc gtcataacta
     4621 aatcggttaa caaactcctt accatcagaa gtcttttctt tttcattatc ctttccttcc
     4681 gccctggtaa acacatagcc cgcacggaca aattgatatt gatatttttc ttcttctttt
     4741 ttcgatgtga taaccctcac atcagaatac cgttcgttga ttttcttttt aagtttgtca
     4801 gcctgttctt tcagcgtacc gtctaaaaac aggatatcct tctctttaag cggcagatgc
     4861 tcctctgcct gatgcttgtc gggaatttcc gtaccgtctt gtttatagga agcaatattc
     4921 cgccttggca gccgcattgc cgcaccgacg gcgggccggt tgaccggcgt ggtttctacc
     4981 gaagacccgg cagggggcgg agtgggaacg tccttagatt tgaaggtgac ggggtacgcg
     5041 gtcggcgttg attcgacaac aggctgcacg ccgaaattgc cgccgataca agatgctaaa
     5101 agtaagggca acaagacaat gccgccataa ttcggtttac acatccctac ttttcctcta
     5161 tttgattaat aataattatc attatattaa tatgtacaga taatatcaag ccgtttttat
     5221 agtgaattaa caaaaatcag gacaaggcga cgagccgcag acagtacaga tacattccgt
     5281 cattcccacg aacctacatc ccgtcattcc cacgaacctg cacc
//
LOCUS       DAWISC010000142         5306 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWISC010000142 DAWISC010000000
VERSION     DAWISC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.142"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(90..1445)
                     /locus_tag="WIW87_001674"
     CDS             complement(90..1445)
                     /locus_tag="WIW87_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ5174804.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGMHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="WIW87_001675"
     CDS             complement(1460..1732)
                     /locus_tag="WIW87_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ5174805.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1857..2594)
                     /locus_tag="WIW87_001676"
     CDS             complement(1857..2594)
                     /locus_tag="WIW87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ5174806.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WIW87_001677"
     CDS             complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WIW87_001677"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ5174807.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3919..4512
                     /locus_tag="WIW87_001678"
     CDS             3919..4512
                     /locus_tag="WIW87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174808.1"
                     /translation="MVDGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDRKGRPDIIAAH
                     KYRAVTKVGNESLNVGVIVREFPDGHKHYDHFILKDE"
     gene            4635..5250
                     /locus_tag="WIW87_001679"
                     /pseudo
     CDS             4635..5250
                     /locus_tag="WIW87_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         1462 a         1372 c         1171 g         1301 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggtgcggga
      121 atcctgccgc cccggttgac gaacacttcg cacgaacctt ctttgaccgg cattacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggttttacc cgttaccgga
      241 ataatgcgca cggcagtggt tttgctgtta atcataccga tggcggcttc gtcggcaatg
      301 atgccagaaa tggtgtgcgc gggcgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc ttcggcggca
      421 gcaatcatgc cttcatcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgca taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaggc ggttttcttc
      721 acaacttcgg caacttcggt caatgtcgtt gcatccgaat tttccaacgc ggcttttacg
      781 acacccgggc cggatacgcc gacattaatc acagcatccg cttcgcccga tccgtgaaac
      841 gcacccgcca taaacggatt gtcttccacc gcattgcaga acacgacgat tttcgcgcag
      901 ccgaaacctt cgggcgtaat ttcagccgtg cgtttgatgg tttcgcctgc cagcttgacc
      961 gcatccatat tgataccggc acgcgtactg ccgacattga tggagctgca cacaatatcg
     1021 gtggtcttca tcgcttcggg aatggaacgg attaacacct cgtctgaagg cgacatgcct
     1081 ttttgcacca acgcggaaaa gccgccgata aaggacacgc cgatggcttt ggcagcctta
     1141 tccaaagttt gcgccacgct gacataggaa tcggcatggg tagccgccgc gatttgggca
     1201 atcggcgtaa cggaaatgcg ctgattcaca atcggcacgc cgtatttggc agacagatat
     1261 tttgccgtcg ccaccaagtc tttgccgacc gtggtaattt tgttgtaaat gttttggttc
     1321 aacacattga tatcgctgct gatgcagtcg tgcagatcaa tgccgatggt aatggtgcgg
     1381 acatcaaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc ggattggata
     1441 ctcatcacat tcctccgact caaatgcggt gcatcgcttg gaagatttct tcattttgca
     1501 tacggatatc aagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcctgcc
     1561 gagatttggg gcatttcgag gtatccacca agataatcat ggtaaaaaaa tcatccatca
     1621 gctgttggct gatattaagg atgttgattc ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttgccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccacca tcggattttg aggaaatatt
     1801 gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacggca tcaaatttag
     1861 ggttcgatta aataaccatc cttatcccac tgggttttcc taaccaactt atcatcctga
     1921 taaacagctt cactcttttt agaaccatct tcataccact ctaagactac tccattacgt
     1981 ttatgatgat ggatagacag ttccgagagt aatcgaccgc tttcatccca tgtcagaatc
     2041 ttggcaggct catcattgac cataaccatt tccgttttga tattgccgtc ggcataccat
     2101 tgcttccata cgccgtttgc cttattctgt ttaaactgga tttcgctttc cttgccgccg
     2161 ttacggtaat agcggtaccc cgtaccttca ctcaaaccat ttttataagg cataacggca
     2221 gattttttac cgttcggata ccagttgacc cactccccgt ccggcttacc cttgctgaag
     2281 ccccccgcca tttttttctg accattaaaa tgccacaaaa tcaacatacc gttttgcagg
     2341 gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat aaggttcgga atatttcttc
     2401 atcgacggat aataaaaatc ctgcgcgtgc gcaataccct ccgccacact atattgcctg
     2461 atataagcgg cagaagacat cgtcgccgtc agtttcccgt tctgattaaa ataaacagaa
     2521 taggtctgcg ccggcaaagc ggccgaaaaa cccaacagga cagttgaaaa tacaatccga
     2581 gataattttt tcattgcaat agcgatataa aaacaaggct gtgttttagt aatcttttga
     2641 tttcaattat ttgcaaggga aaagacaatt attttccggt taggaataaa cctattctat
     2701 tgaatatatt gaagccaagt acgcctatca acactatatt aaaacactgc caaaaacaat
     2761 taacttataa acaatatggt aaggatttct ctgccaagca tcaaacccga gacaacgtat
     2821 cgtaaaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttcccctt caactcactc
     2881 ttcacccaat aactgctcgc gcgtcaagag gaaaacaaaa ccgtcgcccc cgctggtttc
     2941 caaccaagta aaaggcaact ccggatacgc tgcttccaat acatccctgt tatgcccgat
     3001 ttccaccagc aatacacctt tgggattcag aaactttgcc gcattcagaa gaatctgcct
     3061 ggtggcatcc aacccgtccg ccccgctgcc caatgccaat tccggttcgt gcaaatactc
     3121 ttcaggcaat aactcaaccg attccgcatc cacataaggc ggattggaaa caatcaaatc
     3181 ataagtgcct tccaatcctt caaacaaatc cgtatgaata agccggatgc gttcttccaa
     3241 accataatct tcgacattaa tccctgccac ttccaaagca tccaagctca catcaaccgc
     3301 atcaatttgg gcatcaggat aatgatgcgc catctgaatg gcaaggcaac cgcttccggt
     3361 gcaaagatcc aaagcattat gcaccagctc atcgtattct atccaaggac gaagtccgtt
     3421 acccaacaat tcataaataa aagaacgagg tatgattacg cgctcatcca catagaaatc
     3481 aaactctccc tgccatgcct ggtgtgtcaa ataagcggct ggaatgtgtt cgacagcacg
     3541 acgctcaata accgccagca cttcctcttt ttcagcttcc aagagttttg catcaagata
     3601 tggggcaagc atatccaaag gcaaattcaa agtatgcaga atcaaataag ccgcttcatc
     3661 atgcgcatta tctgttccat gaccaaaaaa gagccctgcc tcattaaaac ggctgactgc
     3721 aaaacgtaaa atatcgcgga tagtcgtcaa ttcttgtgct gcctgattaa acataatatg
     3781 aaccattctg cgtatagata cttttaatta taacagaaac aacaagcaaa ccttttcata
     3841 tcgccaaata accacccaat ctacccatac aactacataa atgcccgcgc gaaaaccatc
     3901 gcccgaacgg aaacgacaat ggtcgacggc atgggcaatc tgattggctg ggaaaaaacg
     3961 gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc cgatgtctgc
     4021 aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca cggcgcattg
     4081 acgatacccg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg tggcgaattg
     4141 ggagaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc gcgggataac
     4201 tttatcggca aaagctatgt caataaaaac agcgggcatg aactgaaggt aacttggcaa
     4261 ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat aacaaaactt
     4321 gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataggaa aggtcgtcct
     4381 gatattattg cagcacataa gtatcgtgcc gttaccaagg ttgggaatga gtctttaaat
     4441 gtcggcgtga ttgtaaggga gtttccagac ggccataaac attacgacca tttcatcttg
     4501 aaggatgagt aaagcccttt tgcagtgtag ctctggagcg gataccgtta aggcaagtac
     4561 gcttccagcc ttacaaaagg gctttaaatt cagcatacca tttatacaaa cagaaataaa
     4621 ccaatgacga agttatacgc agaaatcgcc aagatggagg ccaggacgac ggcacggtca
     4681 aagtttgggg ctatgcctca agcgaggcgg tcgattccga cggcgaagtc atcgcggcgg
     4741 cagctatgaa ggcggcgatt cccgattata tgaagtttgg cgcggagcgc gagatgcacg
     4801 gctcaaacgc tgcgggaacg gcaattgaaa tcaacgtgga agatgacggc agaacctttt
     4861 tcgtggcgca tatcgtcgat cccgttgccg tgacgaaggt caaaacaggc gtttacaagg
     4921 gcttttccat cggcggcagc gttaccgccc acgatgagtt gaacaagtcg caaatcacgg
     4981 gtttgaagct gacggaaatc agcttggttg accgacccgc caatcccgat gcggtgttta
     5041 cctgctttaa ggcggacaaa ggtgcggaag cggtaaacaa cgatacagaa cataatgcta
     5101 catattttag ccatttccct tccaaacaaa aaacaaaaaa gcaccggcgg cggccgatgc
     5161 cccttccttt acaggttccc ctatttttta tccgcgggca gagccggttt tgccgggcct
     5221 tttggtgcgg gtgcgccgac ggaagcctga tccttcagct tcgccagcac cgcagggccg
     5281 atgcccttta ccttggtcaa atcgtc
//
LOCUS       DAWISC010000143         5255 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWISC010000143 DAWISC010000000
VERSION     DAWISC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.143"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WIW87_001680"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WIW87_001680"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ5174809.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WIW87_001681"
     CDS             785..1771
                     /locus_tag="WIW87_001681"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ5174810.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WIW87_001682"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WIW87_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ5174811.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WIW87_001683"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WIW87_001683"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ5174812.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGGHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WIW87_001684"
     CDS             4450..5001
                     /locus_tag="WIW87_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ5174813.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1335 a         1462 c         1389 g         1069 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc gaccggcata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cacagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcacgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 cttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctcttgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccaccgt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaatcacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggtccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgttgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcggcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgcctttttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggtgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggcaa ccgcccgacc aacctcattc
     4201 tcatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagt gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg taaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcga aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgtcaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccg
//
LOCUS       DAWISC010000144         5233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWISC010000144 DAWISC010000000
VERSION     DAWISC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.144"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            86..1561
                     /locus_tag="WIW87_001685"
     CDS             86..1561
                     /locus_tag="WIW87_001685"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ5174814.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="WIW87_001686"
     CDS             1558..2103
                     /locus_tag="WIW87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ5174815.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="WIW87_001687"
     CDS             2100..3236
                     /locus_tag="WIW87_001687"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ5174816.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="WIW87_001688"
     CDS             3283..3765
                     /locus_tag="WIW87_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5174817.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..4926
                     /locus_tag="WIW87_001689"
     CDS             3871..4926
                     /locus_tag="WIW87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5174818.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            4923..5216
                     /locus_tag="WIW87_001690"
     CDS             4923..5216
                     /locus_tag="WIW87_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174819.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         1497 a         1607 c         1106 g         1023 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaaggctac aaccgcatcc cgctcgtgca agaactcctt gccgatttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcttttat cggcctgcct tgcagccact atctcaaagc
      301 cagcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgagcaac acgacggcaa
      361 ccccctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccctgcgt acgctcaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatcctcgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcatatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgctac
     1261 cttccctgcc ggcacactct ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaaccg agcaaacgcg gcatctacgg cggtgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcgggcgtgg ttgccgactc cgatccggcc tccgaatggc aggaaacaca
     1501 aaacaaagcc cgagccgtta tacgcgccgc gcagatggtg caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaag tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgttgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcag tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgctcggcat tcttggcggc ggacaattag
     2161 gcagaatgtt tactgttgct gccaaaacca tgggctacaa agtaaccgta ctcgatccca
     2221 acccgaatgc gccggcagcg gaatttgccg accgccattt gtgtgcgccg tttgacaacc
     2281 aaaccgcttt ggaagaattg gcaaaatgtg cggctgttac gaccgagttc gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac ataccaatgt ttcccccagc ggcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggcctgcaaa
     2461 ccgcgccgta tcaagcaatt tgcaaagccg aagacatcac tgaagaaagc atacaatttc
     2521 tgcccggcat cctgaaaacc gctacattgg gctatgacgg caaaggccaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gccgcgtttg ccgaacaccg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggacttgcgc ggcgaaattt ccgttatcgt atgccgtctg aacaatgaca
     2701 acgtgcaaac tttcgatcct gccgaaaaca ttcacgaaaa cggtatcctc gcctactcca
     2761 tcgtcccagc ccgactgagt gccgacattc agcaacaggc gcgacaaatg gcgcagcgtt
     2821 tggccgatga attgaactac gtcggcgtat tggcggtaga aatgtttgtt gtcggcgaca
     2881 cgcatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caattccggc caccataccg
     2941 tcgacgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgctgacac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcgaaccg gattggtttc ccctgcaaag ccggccggac gcgcacctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 gcaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctgct tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctccc cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccgccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagacacc ttctctcccg aaaaaaaatt cggcggatgg
     4861 gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct gaccgcacaa
     4921 aaatgaccgc tatgccgtct gaaccgcctt tcagacggca tttcacacat caaaacagaa
     4981 aaaccgcgcc caaaatcatc atcacaaaac acaacggcaa accggataca aaacccaaca
     5041 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcccgcttc
     5101 aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt gcttttgcct
     5161 gtcagaaaca aaaccaatat tttgcagttc gtatcggaac atttatagtg gattaacaaa
     5221 aaccagtaca gcg
//
LOCUS       DAWISC010000145         5213 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWISC010000145 DAWISC010000000
VERSION     DAWISC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.145"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(366..2642)
                     /locus_tag="WIW87_001691"
     CDS             complement(366..2642)
                     /locus_tag="WIW87_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5174820.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3064..3654
                     /locus_tag="WIW87_001692"
     CDS             3064..3654
                     /locus_tag="WIW87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ5174821.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3718..4776
                     /gene="trpD"
                     /locus_tag="WIW87_001693"
     CDS             3718..4776
                     /gene="trpD"
                     /locus_tag="WIW87_001693"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ5174822.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4928..5143)
                     /locus_tag="WIW87_001694"
                     /pseudo
     CDS             complement(4928..5143)
                     /locus_tag="WIW87_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1211 a         1197 c         1463 g         1342 t
ORIGIN      
        1 cgcctgcgcg ggaatgacga cccattagtt acctaaaatt taaaaaacag aaacctttcc
       61 gcgtcattcc catgaaagtg ggaatctaga acccaaatgc taaggcgatt tatcggaaac
      121 ggctgaaacc gaacgaattg gattcccgcc tgcgcgggaa tgacgggatc ttgggtttct
      181 gcttttgatt tttctgcttt tgcgagaatg acggcgtgaa agtaagaatg atgaaacaaa
      241 aaaaatggga atgatggcat agtggtttgt tctttgtctt tgccatattt cctaacaaat
      301 tgattaaaaa gaaaaaaggt tttcagaatg ccgtctgaaa accttttttg tttgcctgtc
      361 cgattttaaa acttcacgtt cacgccgccg gtaaagctgc ggcccatttg cggcgtatca
      421 gagagaaagc tgctgtgggc gtaaacggat tggttgagca ggttgtcggc tttgacgtac
      481 caattccact cgccatagcg cgtattgcgg cggtagtttg cgccgaggtt gagcatatgg
      541 tgtccgggcg tgcgcgtttc gtagcgggcg agtttgtttt gggcgaacac gcggtagtag
      601 tccaaattgg catcgatacg gtcggtcagc gaggctttca ggtggaagcc gaggcgcgca
      661 gccggaacac ggggggcatt ttggtcgtcc tgtgcgatga aaggacggtt gccgtaggca
      721 tcttctctgc cgggtaggga aggcaggttt ttcagacggc ctcgtacata gtcgccggaa
      781 acgccgatgc ggtagcgcgg tgtcggtttg aagtagattt cgccttccgc gccgtagaag
      841 tcggcgccgg attggttgta gcgcacgagc ttcatttcgc tgtcgtcttc gatggatttg
      901 gggccgcgtc cgtcgtttaa ggtttgggcg taaatgtagt taccgaagcg gttgcggtag
      961 agtgccagat tgtattgcca gcggtcgcct tcgtagccca gcgcgagttc gatattgttg
     1021 gaacgctctt tgttgaggtg tttgttgccg acttcaaagg tgttggtggc gacgtgtttg
     1081 ccgtgtgcgt acagctcttg cgttgacggc aggcgttcct gatgggaggc ggtcaggctg
     1141 agtttgtgtt gtggcgtgaa ataccagttg cccgaaagtg cgaatgagcg ggcggtttgg
     1201 cggtgcgcgc cgaggtcggg cagggggtgg ttgtagtagt tttcccgatc aatcaatgct
     1261 ttgtcgtact gaatggaggc tttttgtttt tccacgcgta cgccgccttc aagcgtgaag
     1321 ttgtcccagt ttgcctgttc tacaccgaaa aagctgtaat gttgcacttt gttgtcaagc
     1381 agcatcggtt gtttaaccgc ttcggatatg gcagataaag cactggattt ttgttgtaaa
     1441 tattgcacgc cccagctgcc tttcagacga cctatgggtt ggtggcgcaa ctcgatgcgg
     1501 gcgttttgcg tttggttgtt aaaaaagttt tcgactgcat cgcctgcttt ttcgtcgtgg
     1561 cggtagtcgt tgcggttcag gtgtacgcgc agggcttcaa aaccggggaa cggttgcttc
     1621 cattcggcac ggagttcgta gcgtttgttg cgcaggtcta tccacggtct gccgctgtgg
     1681 gtgtgtgcgt gtgcattatc gtcgtcgtgg aagccgcagc tcaagcccgg attgtcgtaa
     1741 tcgatgtctt cttcggtcaa caggtgcgga taaagctgta aatagcgttt gttaatcaag
     1801 ctcttttgcc agatgatgtc ggcgtggcaa tcatcgtatt cgtggctgtg ggcaggcaga
     1861 ccatattggt cgcgacggtc gctgtacgct acgccgataa aacctttttc gccaacccaa
     1921 gacagcccga tgctgcccgt ttgcgaatcg gcgtggctgt cgggcaggcg tttcagattg
     1981 cggtaacgcg gtacggcgta atcccccgat ttgcggtaca gcccttccgt gtgcaataca
     2041 aagtttttgc ccaaaccgat attgatgccg ccggacgtga gtttttccag attgccgctg
     2101 ctcaaacgca atccgagttc gcccgatacg ccgttttcag gcattttttc ggggattttg
     2161 ccatcggcaa catcgaccag ccccgccaca ttgcccgagc tgtacaagag cgtaaccggc
     2221 ccgcgcagga tttcgacctg ttgcgacaag gcggtatcta ccataatggc gtgatcgggc
     2281 gaaaaatccg ccatatcgcc tgtttcgccg tgatggttca acactttaat ccgcctgcct
     2341 gtttgaccgc gaatgacggg agcagacgcg ccgccgccgt attgcgaagc gtggatgccc
     2401 ggtacgccgt ctaaagcgtc gcccaagttg acggcttttt ggcgcaaggt atcgccggag
     2461 atgattttgt cggaggcggt cgaagtgtgc aacagccccg acgtggcgcg cggacggctt
     2521 ttgccgacga cgctgaccgt ttccaaatcc accgattgct cagtttcatg cgcttgggcg
     2581 aggaggggtg tgttgattaa aagaattgat aaaacaatgg gtttgagtgt agtttgtgcc
     2641 attttggctt ctcgtcgcat ttcaaaagtt tgttattata taacattaca ttttttatat
     2701 cataagattt tgagaacact cagagggcat aggcaaaagt ttttcaaatg aaacggttgc
     2761 ggcatcgggc ggtgtccatt tgtatccgcc gtccttcggg ggcgcggttg atgttgacgc
     2821 agattccgct gtgtttgccc attatgttcg gctgcgggat aaccaaaatt tatgagtgca
     2881 taaaaacggc acgttcccga acggtcgaaa aggtggcaaa atggcgtact tgtcagaacg
     2941 cgaggctctg cgccaggttc acgagggcgc atcgggcacg ctaagatata agagggtatg
     3001 gatgggggta tcggaaatgc agttaataac aaacaaatta taaatcaata ggttaatcac
     3061 aaaatgcttt tgtttatcga caattacgac agttttactt acaacatcgt ccagtatttc
     3121 actgaattgg ggcaggaagt tgccgtgcgc cgcaacgatg atattacgtt ggaggaaatc
     3181 gaggcattga atccgcaata tctcgttatc ggccccggcc cgtgttcgcc caaagaagcg
     3241 gggatttccg tggcggcgat gcgccatttc gccggccggc tgccgattat gggcgtgtgc
     3301 ctcgggcatc agacgatagg cgaggcgttc ggcggcagga tagtccgcgc caaaacgctg
     3361 atgcacggta aggtgtcgcc cgtgtcccat tcgggcaagg gtatgtttaa gggtttgccc
     3421 aatccggtta cctgtacgcg ttatcacagc ctcgttatcg atcggaatac gatgcccgaa
     3481 tgtttggaag taacggcttg gactgaggac ggcgagatta tgggtgtgcg ccataaggaa
     3541 tatgccgtcg agggcgtgca gttccacccc gaagccctct tgaccgagca cggacatgat
     3601 atgttaaaca attttttaat cgaatttcaa aacttcaaac cgcaaaaaat ctgacgtgat
     3661 gccgtctgaa gcccttcaga cggcattttc gtccgaatat tgaacggagg acaaaaaatg
     3721 attacaccgc aacaggccat cgaacgatta atcagcaata acgagttgtt ttacgatgaa
     3781 atgaccgact tgatgcgtca gattatgaga ggacaggttc tgccggagca gatagcggcc
     3841 attttgacag gattgcgtat caaggttgaa accgtttccg aaattaccgc agctgcagcc
     3901 gtcatgcgcg agtttgcgac aaaagtgccg ctggagaatg cagaggggct ggtcgatatc
     3961 gtcggtacgg gcggggatgg cgcgaaaacc ttcaatattt cgacgacttc gatgtttgtt
     4021 gccgcagcgg caggcgcgaa ggttgccaaa cacggaggcc ggtcggtctc ttcctccagc
     4081 ggtgcggctg acgtggtgga gcagatgggc gcaaacctca acctgactcc cgaacaggtt
     4141 gcccaaagta tcaggcagac cggcatcggg tttatgttcg cgcccaatca ccacagtgcc
     4201 atgcgccatg tcgcccctgt acgccgttcg ctcggtttcc gaagtatttt caacatattg
     4261 ggtccgttaa cgaatcctgc gggcgcgccg aaccagcttt tgggcgtgtt ccacaccgat
     4321 ttgtgcggca ttttgtcgcg ggtcttgcaa caacttggtt caaaacacgt tttggttgtt
     4381 tgcggggagg gcggtttgga tgaaattaca ctgacgggca aaacacgcgt tgccgagctc
     4441 aaagacggaa aaatcagcga atacgacatc cgcccagaag atttcggtat cgaaacccgc
     4501 cgcaatttgg atgaaatcaa agttgccaat actcaggaat ctttgttgaa aatgaatgag
     4561 gtgctggaag gaagagaagg ggctgcgcgc gatatcgtat tgctcaacac cgccgccgcc
     4621 ctgtatgccg gaaatgtcgc tgcttcgctt tcagacggca tatctgccgc acgggaagcc
     4681 atcgattcag gcagggccaa atcgaaaaaa gaggagtttg tcggttttca accacaacaa
     4741 agatgccatt ttcttggaaa gatggagctt gggtgatgcc gccatgatta tggaactttt
     4801 gtggcaaaac ataagcactt cacgaagaga acttaccaaa ctgtttttat ataaaaactt
     4861 ggggctgtac tagataacca gaccaaattc ccattaacta attgtcttaa aatctgaatt
     4921 tgagattcta tttaaaatgc cattggcatt tctttaaatg cagccccaaa tgctctttgg
     4981 gaatgccgtt aaacttacgt aaatggctaa attcactaat atcaagcaca tcataactac
     5041 gaaaggtatc cgtgtataca atgccatcag gcttaacttt ctttcggata attggcaaca
     5101 atgtcgctga ttgcgcatta ggaacaacga cggtataaac cttatatatt gcgtccctaa
     5161 gaagggacga ttaacaaaaa ttaacgtcct ttactttcta caagtaacag ggc
//
LOCUS       DAWISC010000146         5152 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWISC010000146 DAWISC010000000
VERSION     DAWISC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.146"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(61..1317)
                     /locus_tag="WIW87_001695"
     CDS             complement(61..1317)
                     /locus_tag="WIW87_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ5174823.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGSQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETAFLG
                     NLKARKTEFSDSLNTAAELPQWLVEQLKQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDARIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1665..2006)
                     /locus_tag="WIW87_001696"
     CDS             complement(1665..2006)
                     /locus_tag="WIW87_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174824.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2210..3067)
                     /locus_tag="WIW87_001697"
     CDS             complement(2210..3067)
                     /locus_tag="WIW87_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ5174825.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELSVRELLGE
                     HGFRYLLALDADPTRLTDYLAQRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGDTDSPEGMRGFDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLNVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3128..3943)
                     /locus_tag="WIW87_001698"
     CDS             complement(3128..3943)
                     /locus_tag="WIW87_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ5174826.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4007..4177
                     /locus_tag="WIW87_001699"
     CDS             4007..4177
                     /locus_tag="WIW87_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174827.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVRRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            4187..4972
                     /locus_tag="WIW87_001700"
     CDS             4187..4972
                     /locus_tag="WIW87_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ5174828.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         1170 a         1192 c         1408 g         1382 t
ORIGIN      
        1 tgatgaaatg ccgtctgaac gctttgaaaa gggttcagac ggcattttgt tcaaaccggt
       61 ttatttgcgt tgcaatacgg cggcgaagaa gccgtcggtt tggtgtcggg cggagttgag
      121 gcgcaggtat ttgccggtat ccaaatcgat tttcaaaccg gcaagcagtt cggcgcagtt
      181 gacgggttca aattcgggat gttcggacag gaaacgttcg acttgcagct cgttttcttc
      241 gggcaggatg ctgcaagtgg cgtacaccaa acgtccttgc ggttttacca gtttggaggc
      301 ggcatcgagg atgctgtgtt gctgttccaa aaggttggcg acggtttcgg cggattggcg
      361 gtatttgagg tcgggattgc ggcgtaaagt gcccaaaccg gagcagggcg cgtccaccaa
      421 cacacggtcg gctttgcctg ccagtcgggc gatacgggca tcgtgttcgc tgccgatgcg
      481 ttcggggtgg atattggtca gtccggcgcg ggtcatacgc ggtttgaggt tggcaaggcg
      541 tttttcggcg atatcgaagg cgtagattct gcctttgttc gccatttgcg caccgacagc
      601 caaggtttta ccgccggcac cggcacagaa atcgacaatg atttcgcctc gttttgcgcc
      661 caccaataag gcaagcagct ggctgccttc gtcttggact tccagtgtgc cgtctaaaaa
      721 cagttcgtgt ttgttaagcg cgattttgtt tttcaggcgg atgccccaag gcgaataagg
      781 cgttgcctct gcatcggcac tttcggcttg caacagcggc agcactttat cgcgtttgcc
      841 tttcaaagtg ttgacgcgga tgtcgagcgg ggcaggctgg ttgatgctgc ggccgaaagc
      901 gaggatttct tcttcgcgcc aatgctgttt cagttgttcc accagccatt gcggcaattc
      961 tgcggcggta ttcaggctgt ctgaaaactc ggttttacgg gctttcaaat tgccgaggaa
     1021 cgctgtttct tcttcatcaa gcaggtcttt gatttggctg atgttggtgc ttctgccgag
     1081 aaccagtgcg gcgagagcgg ctttgcgcgg ctgcgcgtgc ggacggcgta gggcggtact
     1141 gattttttga tagtggcgca gcgcggcaaa ggcggtttcg gcgatttcgt ggcgatcttg
     1201 actgccgagc tttttgtgtt cgcggaaata ggcggagagg acggcatcgg caggctgttt
     1261 gaaagtcagc atttcagcca aaactttggc ggtatggtcg agttgtgcgg cgttcataat
     1321 gtaatgggtt ctcttactgt gggttattca taatcaatct aacgtcctta aaataacaat
     1381 agcatcttta tcattactta gtattctatc cgcaataaga aagatgtgtt aaaagtgcgc
     1441 aatcgggatt tgtctaaatt tctccgttaa ctacaaaggc agcctgaaaa aagttctatt
     1501 ttcaggtcgc ttgaacagca tccgatataa tcacatcagc gatttcattt tgtaatcact
     1561 ccctaaaaca tgaggaaaaa aacaaaattt attttcttcg ttcagtatat ctatcctaaa
     1621 tatgccatca ctccattgag tttttaattt gtatttcgct cttgttaaat gtttttctgt
     1681 ttgccgattt cttttgctaa tgtaaaaaat actctaccac tctcattggt taaagttttc
     1741 aagtattcat caacatcagt aacattaata acaactgttt catttgtatg tttattaaat
     1801 tctaaatcaa taataatatt cccattaacc aatatttcat atatatcttc ttcttcaggg
     1861 agcatagaca aacaataagc acttgaagct gaaaactctt ttttcagata ctgaagaaga
     1921 aaagtatttt taattaaaat attattaaat ttatctaatg attgcttgac ttctttagtt
     1981 gtccaatttt taacattatt cttcattgta ttctacctgg ggtcgtagtt attaaattac
     2041 catacttatt tgtttggatc gtcataatat tagttggcat gttgttgtta tatttatcat
     2101 atccaatatc cttattaact cggatgactc tttcataaat tgtatgggaa atgagatttc
     2161 catcactaag cctctcaata gatatacgcg ttaatgttgg gaaagggttt cagacggcct
     2221 tcataatgcg ttcgatttcg tgccaaaagc cgtccggccg acattccaaa acggcaatca
     2281 agccttggtc gatacggcgg atttcggttg cgttcaatgt ttcggtttcg gcgacacgcg
     2341 caggcgcgag gtaggccata cggtcgagca gatggtagcg gacttcttgg cgtttaaacc
     2401 cgtattccgc ccaatcttga atatagatgc cgcgccaacc gtatggatta agcggtggct
     2461 caaaagcatg gaaaccgtgt ggaaaaaatc cgatgccgcg cacgatggaa acgggtttta
     2521 cgttaagcgg caaaccgtgc tgccgcaagg tccggatgcc gtctgaagtg tgcggcagac
     2581 cgagttgggc ggtcagtttg gcggcttttc ccaacagcgt gtctttgggg tcgaatccgc
     2641 gcatcccttc gggactgtcc gtgtcgccgc cgtaatattt gcaggtcagc tcgatatggt
     2701 agggtttgcc gttaagcctt gccacaaaat ccgccgcgcc ttgcgtattg ccgtccgaac
     2761 cggaaaccgt gaggttgtgc gcgagcagtt cggcgtgcgg tgcattggca aaccaaaaag
     2821 ccagcagctc ttcggcataa atgccgagac ggtggccgaa cggggcgcgt tgggcgaggt
     2881 aatccgtcag ccgcgtggga tcggcatcca atgccaaaag gtaacggaaa ccgtgttctc
     2941 ccagtagttc tcgcacgctc aattcgcagc cgctttgcca caaaggcggc gcagtcagca
     3001 gcgaggcaag gtcgcggacg ggttggctgg taagtttcca ccataatgcg tctagggcgt
     3061 aattcatgct tatccgaata tcagtcatgc cgtctgaaaa gctggaaatg ttttcagacg
     3121 gccttaatca gtctttttta tggatacggt ggctgacggt attgaaaaaa ccgcgcagga
     3181 tatcgatgtc ttcggtttgc gtattggcgc ggccgaacag gctctgcata cggcgcatca
     3241 gacgctcgcc gttgcggcgg ttgaaaaagc cgatgtcgtt catcacgctt tccatgtggg
     3301 cgaccatgcc tttgatttgc tcgtgggtcg cagcgtggtc ttcttgttga agatgggtca
     3361 tgggcgaacc ggtttggctg aagatttcgt agcacacgac ctgcacggct tgggcgaggt
     3421 tgagcgagaa atagtcggga ttgccgttga tggtcatcag tcggttgcag gcttggactt
     3481 cttcgatgct caagccgaaa gtctcgttgc cgaaaaccag tgccactttc tcgcctcggt
     3541 ttgcggtctg cagtaattcg gataccaaat cgcgcggggt ttgcagcggc gcagtaattt
     3601 cgcggcggcg gctggtcagg gcgcaggcga tggtggtgtc ggcaagggct tcgtccaaag
     3661 aagcggcaat ggtggcattt tccaaaacgt ctgccgcgcc ggaagcgagg atgaagcttt
     3721 cttccggtaa tttaaacgat tgaggatgct ccgggtcaaa cacgggcggg ttttccgtca
     3781 tcggcgttgc catcagattt ggggcgacga tggtcagttt gtgcagaccc attgttttca
     3841 tcgcgcgcgc ggcagagccg atgttggcgg gatggctggt gcgcgtgagg atgatgcgga
     3901 tgttgtccag ataagcgggc agggcgggtt tgagagtagt catgttcggt tttcggtcgg
     3961 aatggaatag ggtataatgc gccgtctttt tcgttcggac ggtcaaatgc cgtctgaaat
     4021 gttctttaac aacgtcggaa atttaacgtt gcgcctgaag cagacacgcg tcaggcggta
     4081 ttttacccga tttggcagga tttaccccaa ccaaataaac tccgattttg ccgctccgaa
     4141 ggactgtacg tccgaaccgg cggtatgcca cccttaagga aatctgatga atccgttttt
     4201 gaatacagcc tttaaagccg cccgccgtgc cggtcagatg atgatacgcg ccgcgggcaa
     4261 tctcgatgcc gtcaaaaccg acagcaaagc cttcaacgat tttgtttccg atgttgaccg
     4321 caattccgaa atcatcctgg ttgaggcttt gaaagaagcc tatccgcacc acaaaatcac
     4381 ttgtgaagaa agcggctccc acggcaaagc cgcagccgag tacgaatgga ttatcgatcc
     4441 gctcgacggc acgaccaatt tccttcacgg tcatccccaa tacgccatct ctatggcgct
     4501 gctgcacaaa ggcgtgttgc aagaagcttt ggtttacgcc cccgaacgca acgacgtata
     4561 tatggcttcg cgtggcaaag gcgcgttgct caacgaccgc cgcatccgcg tttccaaccg
     4621 catcgaactg aaccgctgcc tgattggtac cggcttccct gttgtcgatc aaagcatgat
     4681 ggacaagtat ctggcgattt tgaaagattt cttggcaaaa accgccggcg gccgtcgtga
     4741 aggtgcggct tctttggatt tgtgcgccgt agcaacagga cgttttgacg gctttttcga
     4801 gtttaacctc aaaccgtggg acattgccgc cggtgcattg attgttcagg aagcgggcgg
     4861 tatcgtaacc gatatgtcgg gtgaagacgg ctggctggaa agcggcgata ttgtggctgc
     4921 caatcccaaa gtgctggcgc aaatgttgaa aatcatttcc gcacacgttt aaatttcagc
     4981 ccgctcccgg gaatgcggga aaacgggcgg gtatgccgtc tgaagtatcg cgccgatatt
     5041 tcagacggca tttttactgg cagcgggggc aggaaggtgg aaacggctaa atgcccgaaa
     5101 ctgccgaaga tggaaaccca tatctgatta aacggagaac cctgtacgat tt
//
LOCUS       DAWISC010000147         5095 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWISC010000147 DAWISC010000000
VERSION     DAWISC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.147"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WIW87_001701"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WIW87_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ5174829.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRASNNFPPLKKLITKQL
                     TG"
     gene            complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WIW87_001702"
     CDS             complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WIW87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ5174830.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WIW87_001703"
     CDS             complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WIW87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ5174831.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WIW87_001704"
     CDS             complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WIW87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ5174832.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WIW87_001705"
     CDS             complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WIW87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ5174833.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1302 a         1431 c         1216 g         1146 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgactg
      481 aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc gatacggttg
      601 tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac ggcaaaatac
      661 tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt cgacaactcg
      721 gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt atgaaacata
      781 ccctttccaa ccaacatacc gcttacgaat gcttccatta cggacgcacc atcgcactgc
      841 tgccgctgga agagcacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggcatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgattggcga tgccgccgtc ggtatgcacc
     1081 ccgttacagc acacggtttc aacttggggc tttccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgaacagcgc ggtcaagaca tcggtgcatc ttcattattg gaaaaataca
     1201 gcagcaaaca tatgctgcac gcccatccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgca ccggcaaaac tgttgcgcgg cttggtattg cgtgccagca
     1321 acaatttccc accgctgaag aaactgatca ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttgtgttat gaaccgtttt gcggttttca
     1441 tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaagga
     1501 aacacgataa aaataacaaa gcctccgagc agtcggaggc tttgctttca aactgaaatt
     1561 atttcagttt agtttcttta tacactacgt gtttgcgggc aactgggtca aattttttga
     1621 tttccaattt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac
     1681 ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat taaatagtgt
     1741 ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat gcctactttg
     1801 tcgatggtac gcagtgcagc gttggaaacg cgcaggcgaa cccagcggtt ttcactttct
     1861 acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt gttgttggcg
     1921 tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac tcgtgccata
     1981 atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa acagtgttag
     2041 ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga ttgtcgcatc
     2101 tgtggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt tcgggaactg
     2161 ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat ttcaagtttt
     2221 caaatccctc aatgtccacc gccgccgccg ttgtggaacg gcggttttgc cagccatatg
     2281 acgggtatca tgataatgaa taacaagctg cccgccataa agatttcgtt cgagccgata
     2341 ataaaaccct gctgggtaat ggtattgttg atgatgccta gggtttgaat gtcggaaacg
     2401 ccgtgctggg acaaatgagc ggccgtttcg tgcaatgttg cggaataggg cgtgatgtgt
     2461 tcggcaaagc gtgtgtggtg caacgcttcg cgccgttccc acagggtgct gacgacggat
     2521 acgccgacac cgcccatcag cacgcgcaag aaattcgaca ggctgcctgc ggcggcaatc
     2581 tgcccgccct tcatatgcga cagtgtgatg gtggtcagcg gcagaaaaaa catggcgaca
     2641 ccgacaccct gccaaaactg cggccagatg acgttgccaa tatccatatc ggcataaaaa
     2701 tccgtacgcc aatagaaagt aaaggcaaag gtcaggaagc tggcagttac gaacaggcgc
     2761 atatcgattt tattgccgaa cctgccgatt aacggagaca ggaaaacagg caggatgccg
     2821 acaggtgcgg cggcaagccc tgcccacgtg gaggtatagc ccaggttggt ctgcaacact
     2881 aacggcagca gggtcagcgt ccccatatac accataaaac ccaatgacgt ggcaatgacg
     2941 ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata tttttctccc
     3001 aattcccaaa caataaaata cgacaagcac accagtgcga ctacgcccaa ggtaatgatt
     3061 tctccagagg cgaaccagtc gagttcctta cccctgtcca gcatcatctg taacgcgccg
     3121 ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac ggtttccgtt
     3181 tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat agggatatta
     3241 atgaagaaaa tccaacccca atgccagttt ccggaaatcc agccgccgag tatcggcccg
     3301 agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt ccgttttgcg
     3361 ggcggatagg atgccattaa caggctttgc gacaagggaa tcagcggccc ggcgataaag
     3421 ccctgcaaga tgcggaaaac aaccagcgac tgaaggttgg gggcaatacc gcacagccac
     3481 gatgtgatga cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc gatgcgtttt
     3541 gccaaaaagc ccgtcagcgg cacggaaacg gcgtttgcca cagaaaagga agtgatgacc
     3601 cacgtcccct gagtggttgc cgcaccgagg ttgccggcga tgacgggaac ggcgacattg
     3661 gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag cgtaacccac
     3721 gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc
     3781 agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac
     3841 tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag
     3901 tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc acatcttcgc
     3961 ggttcagcac gatacggacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt
     4021 tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct gccaccctgc
     4081 cgcgataaac aatttgtttg ccgtacaaat cggacaccag ctcggcaggc tgtccgattt
     4141 tcatatgccg caactgcgtc tctttaaaat tagcatccac ccacacatcc gacagcggca
     4201 ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg
     4261 ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac cacgcatctt
     4321 tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc tgaccgccca
     4381 aagccgcacg tgccgaagat tcttccgcca aagccgcttt gaccgccgcc tgcgcctgag
     4441 acactgccgc acgggcgtgt gccagctctt cggcggacac cgcgcccgat tccgccaaag
     4501 cagaccggcg gcgcaaatca tcctgtgcgc gtgccaaatc cgcccggcgc aaggcaacct
     4561 gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc gcctgaacca
     4621 gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat accgccagca
     4681 cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc gtaccgccct
     4741 tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt
     4801 gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg aacagcagcg
     4861 tcaatgccgt caggcggcgt ttgcgtttgg cttgcgtgtt ttgaagtttt gtttcgtccg
     4921 tgtgcgtatc cataagattg ggttccgaat ttcctgattt catataaaca atgttctttc
     4981 aataggaagc agattctata tccgcgcaat aacgcaatca agtcagtatt ttagatgcaa
     5041 tattcaaaaa acaacgcttc aggcggcaaa actgaaaata tatttcgttt atata
//
LOCUS       DAWISC010000148         4886 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWISC010000148 DAWISC010000000
VERSION     DAWISC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.148"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(92..574)
                     /locus_tag="WIW87_001706"
     CDS             complement(92..574)
                     /locus_tag="WIW87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ5174834.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHVGDLVTFYASVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASGDVSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WIW87_001707"
     CDS             complement(727..888)
                     /locus_tag="WIW87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174835.1"
                     /translation="MSEQSEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WIW87_001708"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WIW87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ5174836.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDDGWEEDC"
     gene            complement(1491..2198)
                     /locus_tag="WIW87_001709"
     CDS             complement(1491..2198)
                     /locus_tag="WIW87_001709"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ5174837.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVVTNKNEILASELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2600..3865
                     /locus_tag="WIW87_001710"
     CDS             2600..3865
                     /locus_tag="WIW87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ5174838.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPSVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYAEAIAPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3923..4729)
                     /locus_tag="WIW87_001711"
     CDS             complement(3923..4729)
                     /locus_tag="WIW87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ5174839.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKTDFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1065 a         1108 c         1422 g         1291 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacacg tctccggagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgactgca accatggtga
      241 agtagcagct gttggtatgg cggatttctc ccgtacggat gttttgtgct tcgacacgga
      301 tgccgatttc catagaggta cgccccgtgt agtttacgct ggcgtagaaa gtaaccaggt
      361 cgccgacatg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtgcga aggcagttgg cgttgttgtg tcatagtgta cctgttttaa gaaagggtat
      601 tttgaaatgg gtgtggaaaa attttaggga atgggaaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagcctgcc gaaatgggct gccgatgccg tctgaaggtt tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac taggaaaaac acgagtgcca atacgcctgc
      781 ccaaacgacg agcatgaatc cggcaactgc ctgacccata cggtaaaccg ggtttttgcg
      841 ttcatcttca agaagtgggt tgtgattttt ctcggattgt tcggacattt tattctcaat
      901 cggaaaagtt ggaaatggag gtaaggttac gccgtcatgc aaaaagattc aaggattcag
      961 ttcagcagtc ttcctcccag ccgtcatccc aggcatgttt caatgccgtc tgaacggtat
     1021 cggcatcaaa accgcgatag gcgaggaagc gtgcctgttt ttgtttttct ttaaggtcgg
     1081 cggccggatg tttgaatttt ttacgcaaca cggctatggc ggccagtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgagttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tagcggcgca aaccggagcc gtccgaatgc ctactcttct
     1501 ttgtttttct gaggttgcag gttttcgtaa atttcgggca gcgagccgat aatccagtcg
     1561 gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa ggtaacgccg
     1621 acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc gccgaccata
     1681 accatgtttg caacatcgat acccaaaact tcggcggcgt gccgcagcgg cagggggctg
     1741 ggttttttct cgggcaggct gtcgccgccg agtatcaggc taaaatagtc ggcgagtccc
     1801 agttgtttta gaagctcgga ggcaaggatt tcgtttttgt tggtaacgac ggcgagcggg
     1861 atgcccaaag atttaagcaa tgccagcccg gcttcggttt cgggataggg gcgggtgaag
     1921 acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc ccacagttcg
     1981 gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt gccgatgccg
     2041 tcgcccacat agctttcgac cactttggca ggcagcggtt tcataccgag ttgttccaac
     2101 atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc gtccaaatcg
     2161 aaggcgacgg cttggacgtg ttcgatggct gcattcataa tccggtttcc tgtcgggttg
     2221 aaaaggttta ttttaacatc gtttgcacag ggcttctaat gtatggattg ttaacaatcg
     2281 aaacaggttt tgccggcggt gtttccggat ttcggaacgg agtcgggaag ggtgtgcaaa
     2341 tcccggcgcc gttccaatta aatttttaaa aaataaaagg attcttatat ctttatttaa
     2401 ttggacattt gctttttgga tatgtgttcc gcttttttca gacggattcc gtgcggcttg
     2461 ggaaataaaa tcatatatag attaatcaaa tcgggccgat tccaactaca cgctattaca
     2521 ttttgcttga aaattggtta tagttgcgaa cctgatggct tgttccattc cgaacctctc
     2581 tttgtgaaaa ggaagcaaaa tgtctgaagc ccttgcaaaa gaaaccctca atccgtttga
     2641 aatcgcgcgc aaacaggtga aaaccgcctg tgaccggctg aaaaccgatc cggccgttta
     2701 tgaaatcctg aagagtccga cccgcgtgtt ggaagtcaat tttcccgtca aactcgacga
     2761 cgacacggtc aaaaccttca cgggctaccg ttcgcaacac aacaacgccg tcggccccta
     2821 taaaggcggc gtgcgcttcc atcccagtgt gaatttggac gaagtcaaag ccctgtcaat
     2881 ttggatgacc atcaaatgct gcgttgcggg tattccttac ggcggcggca aaggcggcat
     2941 taccttggat ccgcgcgatt attccgaggc ggaactggaa cgcatcgccc gcgcttatgc
     3001 tgaagcgatt gccccgctga ttggcgagaa aatcgatatt cccgcccccg atgtgaatac
     3061 caacggcaaa attatgtcgt ggatggtgga tgcttatgaa aacgtggtga aacattccgc
     3121 gccgggcgta tttaccggaa aaccggttga gttcggcggc tctttggcgc gtaccgaggc
     3181 taccggttac ggcgtgaacc tcgccgccgt ccaagctttg gaaaaactgg gcaaggacgt
     3241 gaagggtgcc acttacgcca ttcaaggttt cggtaatgtg ggttaccaca caggttatta
     3301 cgcgcatcaa tccggcgcga aagttgttgc ggtttctact gttgacgttg ccatttacaa
     3361 cgaaaacggt ttggatatgg aagctttgtt taaagagttc caagaaaaag gcttcatcac
     3421 caacgaagcc ggttacggca aagaaattac caacgccgaa cttttggctt tggatgtgga
     3481 cgtactcgcc ccttgcgcgc tggaaaacca actgacttcc gaaaacgccg gtaaagtccg
     3541 cgcgaaaatc gtagtcgaag gcgcgaacgg cccgactacg cccgaagccg acgtgatcct
     3601 gcgtcaaaac ggcgtattgg tcgtgcctga tattctggcg aactgcggcg gcgtggtcgt
     3661 ttcctatttc gagtgggtgc aaaacctgca aggctattat tgggagtttg acgaagttca
     3721 agagaaagaa accgtcgtcc tgcgacgcgc gttccgcgat atatggaact tggcgcaaga
     3781 gtatgatgtc gatttgcgaa ccgcgtctta catgatgagt atccgccgtg ttgaaaaagc
     3841 gatgaaactg cgcggttggt attgatgttt gaacaaaaaa tgccgtctga agccttcaga
     3901 cggcattttt gtgcgggcgg gattaatggt gcgaatgacc gatttggaag gttaaagtcg
     3961 agtagttcgc ctgtttttgg cacacgcttt gatcggggaa gtcggttttg tgttcgacat
     4021 tggctttcca gaagccttgg cgcaagggga tgatgtccac ttcgcctttg tcgtctgtgc
     4081 tgtcggagaa agcctgtgct tcggttttgt gcgttttgct gcggtcgctg gtgtcgaagc
     4141 cgtcaaaggt ggcggtaacg gtggcattgg gcagcggttc gccacggaac agaacgcgga
     4201 ctttgaagcg ttcgcctacg tgaatgttgg cgggattgtc cagcgggacg atttccaagt
     4261 tttgtccgac cggtttggtg atgatggcgg tgtccgcgct ttcgtgtccg acgttgacga
     4321 tgtttttgcc gaacattcgg gtttgttcgc aatagcttgc gtcaggcatt tctttgatgc
     4381 ccgcctgttt ccagcctgct ttgttttttg accagaaagt aggctgatat tcggcgatga
     4441 cgaggtaact gccgtcctta acgggacggt tgcttcggta ctggtagttg tatgtgccgc
     4501 gttgaatcat gttttccttg cctttttcgg taaccagctg catcggtttg ctgaagatgt
     4561 gcaggcggtc tttggcgatg ggttcgagtt cgggaaattc gccgtagccc aagtcggctt
     4621 taaggtattc gccgccgtgc gtgtgggcgg tttcgaccca gacgcggtgg gcgtgggcgg
     4681 atgtggcaaa tagggcggaa acggcgagca gtgtcaatgt tttcttcatg gtgcagcctt
     4741 tgtctcgtgt gggttaaaga ttttgttatg ttataacaaa ataggtatcg tgtgaagacg
     4801 gatttgctgt ctgaaaactg tctttttgta tagtggatta acaaaaacca gtacggcgtt
     4861 gcctcgcctt agctcaaaga gaacga
//
LOCUS       DAWISC010000149         4725 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWISC010000149 DAWISC010000000
VERSION     DAWISC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.149"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            164..943
                     /gene="pgeF"
                     /locus_tag="WIW87_001712"
     CDS             164..943
                     /gene="pgeF"
                     /locus_tag="WIW87_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ5174840.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIVAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            995..1474
                     /locus_tag="WIW87_001713"
     CDS             995..1474
                     /locus_tag="WIW87_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174841.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTITRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEETLWAEMRQDAAEQIVRRLTFLKAE"
     gene            1476..2474
                     /locus_tag="WIW87_001714"
     CDS             1476..2474
                     /locus_tag="WIW87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ5174842.1"
                     /translation="MVAHIGRIDTDTPLKSLYVIHGEEELLRIEALDALRAAAKEQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAACLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDIRTLIRLAASLKQGQSIQSVRNSLR
                     LWGEKQTLAPIAVKRISVVRLLDALKTCAKIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2595..3014
                     /locus_tag="WIW87_001715"
     CDS             2595..3014
                     /locus_tag="WIW87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174843.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRLFVERLKKFDIDPEKGKVNEANLRRMYHSGGQYQKDVVTLICLS
                     QKCSVDEAHAMFKKRPSRQEINQMAAKQSRGQKRPHR"
     gene            3089..3604
                     /locus_tag="WIW87_001716"
     CDS             3089..3604
                     /locus_tag="WIW87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ5174844.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3688..4572)
                     /locus_tag="WIW87_001717"
     CDS             complement(3688..4572)
                     /locus_tag="WIW87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ5174845.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIVRGKVECKIQIQDAENGSQSLELNRDLVGQLAEINQTLR
                     KHHDLAKLGVADILRFPGVLASQRESTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1058 a         1235 c         1314 g         1118 t
ORIGIN      
        1 aagggcatca ttcttgcacc ggcaagaatc caaacccgaa cgtttgaaaa caatcccgaa
       61 tctccgaatt cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg
      121 cagttaatca accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga
      181 aaccctaaat cttgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc
      241 caatgtgaaa accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag
      301 tttgaacctc ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat
      361 cgtgcaacag caggtcggat tgcccgttgc ctacctcaat caaatccaca gcaccgtcgt
      421 cgtcaacgct gccgaagcgt tgggaggcac acccgatgcg gacgcttccg tggacgacac
      481 gggcaaagtt gcctgtgctg tcatgaccgc agactgtctg cccgttctat tttgcgacag
      541 ggcgggtacg acggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt
      601 gcaaaacacc attgtcgcga tgaaggttcc gcctgtcgaa atgatggcgt atctcggccc
      661 cgccatcagt gcggatgcgt ttgaagtcgg gcaggacgtg tttgatgcgt tctgcacgcc
      721 catgcccgaa gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct
      781 ttacgcgctc gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtgt acggcggcac
      841 gcattgtacg gttttggaac gggacacttt cttttcctac cgccgcgacg gagcgacagg
      901 gcgtatggcg agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa
      961 tctaccgact ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc
     1021 agtgctgatg ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc
     1081 gctgacctac cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac
     1141 cgcgctgtat caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg
     1201 tatagacagc gtttcccaaa acaaggaaac ctataccatt acccgtgcgg cagtcatcaa
     1261 cgaatatctt ttaatcttga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg
     1321 caaaccgatg accgtgtccg tccgccgcgt tcttgcttat gcggacaacg aaattttagg
     1381 caaacaggaa gaagaagaaa ccctgtgggc ggaaatgcgg caggatgccg ccgaacaaat
     1441 tgtccgccgc ctgacctttc tgaaggcgga atgacgtggt ggcacatatc ggacgcatcg
     1501 acacggacac gcctttgaaa tctctgtacg tcatccacgg cgaggaagaa ctgttgcgta
     1561 tcgaggcatt ggacgcattg agggcggcgg caaaggaaca gggctacctc aaccgcgaag
     1621 cctataccgc cgacgcgtct ttcgattgga acgaactgtt gcaaactgca ggcagtgcgg
     1681 gtctgtttgc cgatttgaag ctgttggaac tgcatatccc taacggcaag cccggcaaaa
     1741 ccggcggcga ggcgttgcag gattttgccg cctgcctgcc ggaagatacg gtaacgctgg
     1801 ttttgctgcc caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg
     1861 caaaggggga agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac
     1921 gcggacggct ggacaaaatc ggtttgggta tcgaggcaga tgcattggca ctgtttgccg
     1981 agcgcgtgga aggcaatttg ctggcggcac gtcaggaaat cgacaagctc gggctgctgt
     2041 atccgaaagg gcataccgtc aatatcgatg aagcgcaaac cgccgtcgcc aatgttgccc
     2101 gcttcgacgt gttccaactg gcaggcgcgt ggatgaaggg tgatgtgccg cgcgtatgca
     2161 ggttgttgga cggattgcag gaggaaggcg aagaaccggt gctgttgctg tgggcggtag
     2221 ccgaagacat caggacgctg atccgtctgg ctgcttcctt gaaacagggg cagagcatcc
     2281 aatccgtccg caacagtctc agactttggg gtgaaaagca gacgctcgct ccgattgcgg
     2341 tcaagcggat ttccgtcgtc cgcctgcttg acgcgctcaa aacctgtgcc aaaatcgaca
     2401 ggattatcaa aggcgcggaa gagggcgacg catggacggt attcaaacgg cttgtcgtgt
     2461 cgctggcgga ataaagcggc aatccctaaa atccgaaaat actgtaaaat accgttaatc
     2521 ctgaaaagta ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa
     2581 ggaacatcaa ccctatggac aataagacca aactgcgctt gggcggcctg attttactga
     2641 ccaccgccgt tttaagcctc attatcgtat tgattgtcga ttcctggccg cttgccatcc
     2701 tgcttgccgc catcatcgtc gctgccgccg cgggcggttt tgtttggacc agccgccgtc
     2761 agcagcgtct gtttgtcgaa cgtctgaaaa aattcgatat cgatccggaa aaaggcaaag
     2821 tcaatgaggc caacctgcgc cgtatgtatc atagcggcgg acaataccag aaggatgtcg
     2881 ttactttaat ctgcctgtca caaaaatgtt cggttgacga ggcgcatgcc atgttcaaaa
     2941 aacgtccgtc ccgtcaggaa atcaaccaaa tggcggcaaa acagtcgcgc ggtcagaaac
     3001 gtccgcaccg ttaaccgtcc gaggcatatc tgtatgaatg ccgtctgaaa cctgttggcg
     3061 tttcagacgg catattctga ttgaaaagat gatgatactg aaaaccgccc cgctcaaacg
     3121 ccgctttgcc gccatgctgt atgagatgct gctggtcggt gcggcaacct gtttggcagc
     3181 attgtttgcc ggtattgccg ccatttttct gaatcccgtt tctatcgcgg tttctgcatt
     3241 ggtaacgagt atcctgataa tgggatcatg gtggctttat tttcgcgcca actggcacgg
     3301 tcaggggcag accttggcga tgaggacatg gaaaatcggc ttgtgcgacc ttaacggcat
     3361 acagccgtct ttgcacctgc tgcgcctgcg ctttatttgg gcgtgcatat ttatcgtctt
     3421 cattcctatg cttgcttatg ccggattacg ccacttcctc ggcattccgc ccaagggcgc
     3481 ggccggcgcg gcattgattt ggctgatttt accgtggggg ttcgcactgc tgaatcccga
     3541 tcggcagttt ctgtatgatt ttcttgcagg aacaagattg gtggcggtca aaggaaagcc
     3601 ttaagccttt ataccgcaaa ggtttcaacc tgaaaaaatg ccgtctgaaa gggctttcag
     3661 acggcatttg cttatcgggg aaaccgatta ttcgatattc tgcacctgtt cgcgcatctg
     3721 ttcgatcagg actttcagct cgaccgaggc ttgggtgcat tcggtagcga tggatttgct
     3781 gcccaaagtg ttggcttcgc ggtttaattc ctgcatcagg aagtccagcc gtttgccgct
     3841 gctgcctttg tgttcggtaa cgatacggcg cacttcggca atgtgggtgc gtaggcggct
     3901 gaactcttcg tcgatgtcgg atttttggat aaagagggca aattcctgtt gcaggcggtc
     3961 gttgtcgatg ctgccgaccg cttcgacgag gcgggcgcgg attttttctt tatgtgtttc
     4021 caacagggta ggaaagagtt cgcttaatgc atctatgatt tcttccatag cctcaaggcg
     4081 ttgcagcagg tgttcgccta atttcttacc ttcccgcttg cgtgcggcag tgaagtcttt
     4141 taacgctttt tcggtcagtt cggtaatgct ttttgccaat tcttccgtac tttccctttg
     4201 gcttgccaat acgccgggaa aacgtaaaat atcggcaacc cctaattttg ccaggtcatg
     4261 atgtttacgg agggtttggt tgatttctgc cagttgtccg accaaatcgc gattcagttc
     4321 caaggactga ctgccgtttt ccgcatcttg aatttggatt ttgcattcga ctttgccgcg
     4381 cacgatatgg gatgaaattt tctcgcggat accgctttcc aaatagcgca aatcatccgg
     4441 catcctgatt tgaatgtcca aaaagcggtg gttgacggcg cggatgtcga gattgatgcg
     4501 tttgctgccg cactctgccg ccgcgttggc aaatccggtc atgctgtgga tgtggatatt
     4561 tccgctgctc atgtcgttct ccgaagcccg ttaaaatgga atcaatatat cacatctgta
     4621 tggcggcaag cgttttcggg tgtgaaaaat tgaagatttt gcagcggcag attggaatca
     4681 cgcgcttttg ttgctgcaag gaagggaaat gtatagtgga ttaac
//
LOCUS       DAWISC010000150         4654 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWISC010000150 DAWISC010000000
VERSION     DAWISC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.150"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            116..1507
                     /locus_tag="WIW87_001718"
     CDS             116..1507
                     /locus_tag="WIW87_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ5174846.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1569..2321)
                     /gene="rlmB"
                     /locus_tag="WIW87_001719"
     CDS             complement(1569..2321)
                     /gene="rlmB"
                     /locus_tag="WIW87_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ5174847.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(2388..2876)
                     /locus_tag="WIW87_001720"
     CDS             complement(2388..2876)
                     /locus_tag="WIW87_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ5174848.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2881..3600)
                     /locus_tag="WIW87_001721"
     CDS             complement(2881..3600)
                     /locus_tag="WIW87_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ5174849.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMCEALRQ
                     AEQSSADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3604..4545)
                     /gene="miaA"
                     /locus_tag="WIW87_001722"
     CDS             complement(3604..4545)
                     /gene="miaA"
                     /locus_tag="WIW87_001722"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ5174850.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTAYSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDCTSCTRLIEAAKRFFGA"
BASE COUNT          865 a         1115 c         1506 g         1168 t
ORIGIN      
        1 aatgtggtaa acgtgtggac cagacggatg ccgtctgaaa tgcaaattga agccgtgcgg
       61 cagattcgct acaatccgcg cttggatttt tcaaccttta aaataaggaa atacaatgag
      121 cggtcagttg ggcaaaggtg cggatgcgcc tgatttggtg tacggtttgg aagacaggcc
      181 gccgttcggt aatgcgctct tgagcgcggt tacccatctt ttggcgattt ttgtgccgat
      241 gattacgccc gcgctgattg tgggcggcgc gctggaattg ccggtggaga tgacggcgta
      301 tctcgtgtcg atggcgatgg ttgcgtcggg tgtcggcact tatttgcagg tcaaccgctt
      361 cgggccggtc ggttcgggga tgctgtccat ccagtcggtg aatttttcgt tcgttaccgt
      421 gatgattgcg ctgggcgcgg ggatgaaaga gggcggtttg actaaggatg cgatgatttc
      481 gacgctcttg ggcgtatcgt ttgtcggcgc gtttttggtg tgtttctcgg cgtggcttct
      541 gccgtatttg aaaaaagtga ttacgccgac ggtcagcggc gtggtcgtga tgctcattgg
      601 tttgagtttg gtacacgtcg gcattaccga tttcggcggc ggcttcggcg cgaaggcgga
      661 cggcacgttc ggctcgatgg aaaacttggg gctggcatcg ctggtgttgc tgattgtgtt
      721 ggtgttcaac tgcatgaaaa acccgctgtt gcgcatgagc ggcattgcgg tcgggctgat
      781 tgccggctat atcgtcgcgc tgtttttggg caaggtggat ttttccgcgc tgcaaaacct
      841 gccgctggtt acgctgcccg taccgtttaa atacggtttt gctttcgact ggcacgcgtt
      901 tattgtggcg ggcgcgattt tcttgttgag cgtgtttgag gcggtcggcg atttaaccgc
      961 gacggcaatg gtgtccgacc agccgattga aggcgaggaa tacaccaaac gcctgcgcgg
     1021 cggcgtgttg gctgacggct tggtgtcggt gattgcgacg gctttgggtt cgctgccgct
     1081 gacgacgttt gcgcaaaaca acggcgtgat tcagatgacc ggcgtggctt cgcgccatgt
     1141 gggcaaatat attgccgtga ttttggtgct gttgggtctg ttccccgttg tcggtcgcgc
     1201 gtttacgacg attccgagtc cggtgttggg cggcgcgatg gttttgatgt tcggcttaat
     1261 tgcgattgcg ggcgtgcgga ttttggtcag tcacggcatc cgcaggcgcg aagcggtgat
     1321 tgcggcaacg tcggtcggtt tgggcttggg tgtcgcgttt gagccggaag tgtttaaaaa
     1381 cctgcccgtc ttgttccaaa actctatttc cgccggcggc attacggcag tcttgctgaa
     1441 tttggtcttg cccgaagata aaaccgaggc ggcggtcaag tttgataccg accacttgga
     1501 acactgattt tgaaaatgaa tgccgtctga aacagaatcc ctgtttcaga cggcattgtt
     1561 tttgaggctt atactttttc gttttttaat acgcgttgtc ggcgtgtttc acttaatacc
     1621 attccggcag acacggagac gttcatgctt tcgactgtgc cgaacatggg tatagacacc
     1681 agcatgtcgc aatgttcgcg cgtgaggcgg cgcataccgt cgccttcgtt gcccattacc
     1741 cacgccgcgc tgtcgggcag attgcaatgg taaaggtcgg actcgccgct catatcggtg
     1801 ccgataatcc aaatgccgta ttctttcaat tcgcgcaggg tgcgggcgag gttggttacg
     1861 gtgatatagg ggacggtttc cgccgcaccg caggcgactt tgctgacggt ggcgttcagc
     1921 cccgcgcttt tgtctttcgg tgcgatgacg gcgtgtacgc ccattgcgtc ggcggtacgc
     1981 aggcacgcgc cgaggttgtg cggatcggtg atgccgtcga gtatcagcag cagcggcggt
     2041 tcgctgaggt tttccaatac gtcttcgagg tggacgtggt ttttggaggc atcgataaat
     2101 ccgaccacgc cctgatggcg cgcgcctttg ctgatggcgt tgaggcggtc ggcatcggca
     2161 aaatatacgc ggatgttttc gtttgccgcc ttttccaaca cttcgcgcgt gcgtgcgtct
     2221 gatttgcctt cttggatgta gagttcgacg atggatttgg ggttttgcca caatcgggcg
     2281 ttgacggcgt ggaagccgta gatgggtctt tggtttgcca tgatggtgct ttgtaaaaag
     2341 ggttcagaca gcattatagc aatttgccgg tatgccgtct gaaagggtta aaacaggtag
     2401 gcgatgtatt tcaccaacag gataaacaag atggatacgg cgcagccgat tttgaacgcc
     2461 gtgccgacga caagccccaa cagcgtaccc aagcccgctt tacctgcctg aagcatattg
     2521 cgccgttcga tcagttcgcc tgccgccgcg ccgataaagg gaccgagtat tagtccggga
     2581 agggagaaaa atatgccgat gatgctgccg gccaatgcgc cgcgaacggc gagcttgccc
     2641 gctccggtat attttgtccc ccatatgcct gccacatagt ccgccagtat gccggcaagg
     2701 ctgatgagtc cgaccgtcca caaaacgccc gcgccgtaga tttggtagcc gccggcatag
     2761 gcaagcagcc atgttccggc aaacatcaat gccaatccgg gcagggcggg gtaaacgatg
     2821 cccgccgtgc cgacggctat cagggcgagg gcgaggatga cggtcagtac ggtcataggt
     2881 tcaacctttt cttttgtttt gaaaaaaacg gcttaacacg gcgcggcatt cttcttgcag
     2941 gattccgccc cgtatggcgg tgtgcgtatt gaggcgtttg tcggcaaaca ggttgacgat
     3001 gctgcctgcc gcgccggttt tgggttctgc cgccccgtag atcacacgcc tgattcgtgc
     3061 ctgtatcagt gcggacgcgc acatggcgca gggttcgagg gtgatatata tgtcgcatcc
     3121 gtcaaggcgg tagttttgta tttctctgcc tgcctgtgcc aaggcgttga tttcggcgtg
     3181 tcggctgaca ttgcagtcgg caatgcaggt gttgtgtgcc gatgcgatga ttttgccgtc
     3241 tgaaacgatg actgccccga cgggtatttc gccgtcggcg gaggattgtt ctgcttggcg
     3301 cagtgcttcg cacatgaagt gttccatttc ttcctgcggc ggaaaggcgg cgacgggcgg
     3361 atggtttttt aactcggcaa gcaggcgggc tttatgcgct tgggacattt cttgcggcgg
     3421 cgtgccgtcc agcagcgact cgagttgcca cagtgtgctt ttcgtgaggg tcaaacccga
     3481 tgctttgagc agcagaaagg ctttgaccga accgttttgc cgcagttctt cgagtgtacg
     3541 gataccgagc ctgtgcaggg cggcgacggt tttgggggcg agcggcggtg tggtcagcat
     3601 ggtttatgcg ccgaaaaacc gttttgccgc ctcaatcagg cgtgtgcatg aagtgcagtc
     3661 tgaaaacggg tcggcaacgc agtctaaagg tgttttgcgc aaccaagtca gttggcgttt
     3721 ggcaagttgg cgggtggcgg caatgccttt ctcgacaaat gccgggaaat cggtttttcc
     3781 atccagatat ttccatgcct gacggtagcc gacgcagcgg atggcggggg agtaggcggt
     3841 caggccggga tagcggcggc gcaggttttc tacttcgccg ataaagccct gttcaagcat
     3901 caggtggaaa cgcagggcga tgttttcatg caggcgggca cggttttcgg gaatcagggc
     3961 ggcggtatgc aaatcaaaag ggagcgtatg ggaggtcagg ctgccgagat gtgtgctcat
     4021 cggtttgccg gttaaataat aaacttccaa agcgcgtccg atacgctggc tgtcgttcgg
     4081 tttcagacgg catgcggttt cagggtcgac tttttgcagg gtgcggtaga ggaaatccaa
     4141 gccgtacatc tgtttttgtt cgtccaagtc ggcacgcagg caggcgtcgg cttcgggcaa
     4201 atcgttcaaa ccttgggtca gggcgcggaa atacatcatc gtgccgccga caataagggc
     4261 aaacctgccg cgtgaggaaa tttccccgac caagcgcgtg cagtcttcga caaagcgggc
     4321 ggcgctgtat gattcggtag gcgggatgat gtcgataagg tggtgcggga caaaggcgcg
     4381 ttcggaggcg gacggtttcg ccgtgccgat gtccatatcg cggtaaacca gcgcggaatc
     4441 gaggctgatg atttcgacag gcagggtttc ggcaattttg agggcgagcg cggttttgcc
     4501 tccggcggtc ggcccgagca gggcaaaggc tttcggggtc ggcataacgt ttcaggtttg
     4561 gaaaaatacg gattatagcg gaaagcgtgc cgacgttata ttttggtttg cggaagcacg
     4621 ccgacggcaa gggggcgtgt ttaccgtatg cctt
//
LOCUS       DAWISC010000151         4611 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWISC010000151 DAWISC010000000
VERSION     DAWISC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.151"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(67..291)
                     /locus_tag="WIW87_001723"
     CDS             complement(67..291)
                     /locus_tag="WIW87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ5174851.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WIW87_001724"
     CDS             complement(292..1680)
                     /locus_tag="WIW87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ5174852.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WIW87_001725"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WIW87_001725"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ5174853.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2594..3037)
                     /locus_tag="WIW87_001726"
                     /pseudo
     CDS             complement(2594..3037)
                     /locus_tag="WIW87_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WIW87_001727"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WIW87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ5174854.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         1045 a         1403 c         1223 g          940 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact catcatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 ggaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaaa tccggcaggg tttccactcc tgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcatttttag gcagttttga caagcccaac catttatcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttggtttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagtc cggcatcaat ccgcaaagtc ggttgcccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tcgagcgcag tatcgttgcc tatcatggaa
     3241 acgtgtttca gcacttcggg gccgttgccg atgatggtcg cgcctttgac gggatattgc
     3301 agcctgccgc cttccaccca ccacgcttcg gacgcactga acacgaactt gccgctggta
     3361 atgtccactt gtccgccgcc aaagttgacg gcgtaaatgc ccttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg
     3481 gcggaagcgt aactttcgcg gcggccgttg ccggtggact gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcaccctga
     3661 tcgacgacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gttcccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaacc gatgaaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga taacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatatagtgg attaacaaaa a
//
LOCUS       DAWISC010000152         4577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWISC010000152 DAWISC010000000
VERSION     DAWISC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.152"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(279..386)
                     /locus_tag="WIW87_001728"
                     /pseudo
     CDS             complement(279..386)
                     /locus_tag="WIW87_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIW87_001729"
     CDS             complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIW87_001729"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ5174855.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIW87_001730"
     CDS             complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIW87_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ5174856.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIW87_001731"
     CDS             complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIW87_001731"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ5174857.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3178..4410)
                     /locus_tag="WIW87_001732"
     CDS             complement(3178..4410)
                     /locus_tag="WIW87_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ5174858.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1022 a         1313 c         1257 g          985 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat tacataatca tcgcctcaat
       61 atttaatatc ccaaaatata gatttaaaac catcgccttt gtggtttgtc agcattccta
      121 attgagatag aaagtcaaat aatttcttgt ttctccggct gccatcaaga tggtttagat
      181 taagacctcg aacaatacaa tacccaatac tctgtactgt attgtgaatt tcttctatca
      241 aactattatt gtttgcagat aaaatatact tttgattcat atagtgaatt aaatttaaac
      301 cagtacggcg ttggcttgcc ttgccgtact atttgtactg tctgcggctt cgccgccttg
      361 tcctgattta aatttaatcc actataacat atccctgtcg tgaaatgctg tctgaaaggg
      421 tttgcctgcc cttacggcag cagccttgcc ctgaacatcg cctccttcat ttggcgcacg
      481 gcttcgggca gtccgaggaa gagggcttgg gcaatcagcg aatgcccgat gttcagttcg
      541 cggatggcga ggatttgggc gatgggggta acgttgtgta tggtcagtcc gtgtccggcg
      601 ttgacgacca agcccaaatc gccggcgaaa tgcgcgccgt tttggatgcg ctcgaactgc
      661 ctgatttgtt cggcgtggct gcgcgcgtcg gcatacgcgc ctgtgtgcag ctcgacaacg
      721 ggcgcgccga catcacgggc ggcttggatt tgcctgtcgt cggcatcgat aaacaaagac
      781 acgcgtatgc ctgcgtcggt caggattttg gtgaacccgg cgattttttc ctgttgcgcc
      841 aatacgtcca aaccgccttc ggtcgtgatt tcctgacgtt tttcaggcac gatgcacacg
      901 tcttccggca tcactttcaa agcgttttcc aacatttctt ccgtcaacgc catttcaagg
      961 ttcaggcgcg tgcggatggc gtttttgacg gcaaacacgt ccgcgtcttt gatgtggcgg
     1021 cggtcttcgc gcaggtgcat ggtaatcaaa tccgcaccgt gcgtttcggc aaccagtgcc
     1081 gcctccacgg ggctgggata agtcgtaccg cgcgcattgc ggacggtggc gatgtggtcg
     1141 atgttgacac ctaaaagcat aatctttcct tttatttctg ccttcagacg gcatttgaag
     1201 ccgtgccgtc cgaagtcggg acggtttccc gggcggtttc tttgcggtca aactgccgta
     1261 tctgttccaa cacctgccgc gatttcagcc cctcgggcaa caggtggcgg ataaaaagcc
     1321 gtgtgatttt caatgcctgt tgcaggcttt cggcagtgcg gaaactgcct tcgcgcaaat
     1381 cgatcaggct ctgcccgggg gcgacaacgc cggcggcgtg cggcggtacg gcaaatcctt
     1441 ttccgacggg gaagacggct gtttccgggc ggacaaggta tgtgccgcct gccgcaatcg
     1501 tcccgccgtc cccgtcgcgg ttcaaatcgg gggcaacgcc caacaggttc agcagccgcc
     1561 actcgaaacg gcgcaagtcg tcgatataag cggctttgca gcacaccgcc tccatcactt
     1621 ccgccaacgc gtcgtataac tcgggcaccg ggtcttcgcg ggcggtcagt ttcaacacca
     1681 actcgttcac atacaatccg ccgaacaacg ccctgccctg aggctgccgc caaccgccga
     1741 cccattcggc gcggtgtagg gttttgagtt cctgactgcc gtaccacgac acgctgacgg
     1801 gcacgaacgg caccaatacg ccgcgcagct cgctctgcct tttgcgcgcg ctgcgcgcca
     1861 gcaaagccac acgcccgtaa cggcggctga atgcttcaac ccacaggctg ctttcgcgcc
     1921 agggcgaaga tgccagcata aaaacgggtt catggttgac tcggtattcg gacataatcg
     1981 ttggggaaat gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga
     2041 tgactttgac gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt
     2101 cccggcggtg tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttgctcg
     2161 gacggctctc aaacttggtc atggaaatac ccgattcggt cagcggttgc agcagcgagg
     2221 caaccgcgcc tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg
     2281 caccggtttc gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga
     2341 tgcactcggc aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg
     2401 caaccgtacc gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg
     2461 caatccgttc ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct
     2521 gcgcgtggga aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt
     2581 tgtggtggat gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt
     2641 ctaacgtgcg accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg
     2701 cctgacgcgt ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg
     2761 cggcgtgtcc gaaatgtttg attgccgcct gctgggtaaa cgtgccctgc ggccccagat
     2821 aggcgatggt cagcgggcgt tcgacggcga ggcactcgct catcacttcc cgaaacaggc
     2881 gtgctaccga ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg
     2941 cgacttcgcg ttcggggcgg tacactgcgc ccgtgccttt cagctcgccg atggcgtggg
     3001 cgtgttgcgc acgttcgttg agcaggcgca ggatttcggc atcgatggtg tcgatggcgt
     3061 tgcggtgggg aaggaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat
     3121 ttatcgtgca ttctaacatc aaatgccgtc tgaaacggtt tcagacggca ttttccgtta
     3181 aaggtattcg ctttgcccgt tttctttcca cgcacgatgc gagcagagcc acagaagcgc
     3241 aatgccgcaa gaggtggacg cggtcatcgt tgccgccatc acggttgccg aaccgtcgtg
     3301 caagaaggtc gccgccatac ccacccccgc gccgattaaa gattggaata cacccaatac
     3361 ggcgtttgcg ctgccgccct cttctttgaa ataggacata aaacacgcct gcgtgtttgc
     3421 accgaccaag ccctgcgtac cgacggaaaa catcacgcac gcgaccagca gccaaaacgg
     3481 gggcaacccg aaaaacagca cggcggcgag ttgggacagg ttggcggcaa actggacgac
     3541 aatcccccac agcaggatgc tttgcggatg cacgccggtt ttgagccgcc acgcggtaac
     3601 gcggttgaaa aacatcatcg tgatgatgtt gagtgcaaac gcccaagcgt attgatgagg
     3661 cgtaacacgg tagagctgct ggtacacgaa ggaagattcg gtcagaaagg cgaacatcga
     3721 accgaagctg aatgcctgaa aaaacagata acccatcgca gcacgggttt tcaatacgcg
     3781 cttgaaccgc cccgccacca gcccgaacac gtcccgtccg attttgccgc cgacggcggg
     3841 cttgggcagg aaatactgta ccaaaccgag cagcaccagc gaatacgccg ccagaaaaac
     3901 aaaaatcgcc tgccagccac ccaagccctg caacaatgcg ccgaccatgg gtgcgaccag
     3961 cggcacaacc atcaaaatga tgccgataag ggcaaacatc tgggcggctt tgcgtccgga
     4021 ataataatcg cgcaccattg cgccgacgat gaccacagtc atgcccgcac cgaatgcctg
     4081 cacgacgcgc aggttgagga gctgttcggc actcgaaaca aatacgatgg cggcaacggc
     4141 aaggcaatat acaatcaaac cggtcagggc gacgggtttg cgccctttga tgtcggacac
     4201 cgaaccgccg accacctgtc cgaacgccgt gccgaacata aacaaactca aactctgttc
     4261 gatgcggtga acatccgcgt tcagcgattg cgccatttcg ggaatcgcgg gcaggtaggc
     4321 atcgatggaa aacggcatca gcgtaaccag catcgccatc aaaaccgcca tcagtttttc
     4381 gctcatttca ggataatggg cagaaggcat aaattctcgt ccgattcgca aacaaaccgc
     4441 ccggcggcgg aaaacacaat acgcggaaac ggaaaatttt aacacaccgg cacaaacagg
     4501 cggcaacaaa agccccggca aaaccggacg aaccggaacg atatagtgga ttaacaaaaa
     4561 ccagtacagc gttgcct
//
LOCUS       DAWISC010000153         4552 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWISC010000153 DAWISC010000000
VERSION     DAWISC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.153"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(418..1497)
                     /gene="apbC"
                     /locus_tag="WIW87_001733"
     CDS             complement(418..1497)
                     /gene="apbC"
                     /locus_tag="WIW87_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ5174859.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDFAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            1713..1788
                     /locus_tag="WIW87_001734"
     tRNA            1713..1788
                     /locus_tag="WIW87_001734"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1746..1748,aa:Lys,seq:ttt)
     gene            1867..2376
                     /locus_tag="WIW87_001735"
     CDS             1867..2376
                     /locus_tag="WIW87_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ5174860.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2717..3157
                     /gene="nadR"
                     /locus_tag="WIW87_001736"
     CDS             2717..3157
                     /gene="nadR"
                     /locus_tag="WIW87_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ5174861.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3272..3772
                     /gene="hpaC"
                     /locus_tag="WIW87_001737"
     CDS             3272..3772
                     /gene="hpaC"
                     /locus_tag="WIW87_001737"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ5174862.1"
                     /translation="MADLQKNFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3788..4483
                     /locus_tag="WIW87_001738"
     CDS             3788..4483
                     /locus_tag="WIW87_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ5174863.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDSSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
BASE COUNT          969 a         1075 c         1383 g         1125 t
ORIGIN      
        1 gtcttttata atctttgaat attactgttg ttctaaggtc tggattcccg cctgcgcggg
       61 aatgacgaac ccatccatac ggaaacctgc accgcgtcat tcccacgaaa gtgggaatct
      121 agaatctcgg actttcagat aatctttgaa tattgctgtt attctaaggt ctggattccc
      181 gcctgcgcgg gaatgacggc ggagcggttt ctgttttttc caataaattc ctaaaactca
      241 aaatttcatc attcctacaa aacagaaaac caaaatcaga aacctaaaat tcgtcattcc
      301 cgcgcaggcg ggaatccgga acgtaaaatc tcaaaaaaac cgttttatcc ggcaagtttg
      361 cccgccggca ggcgcggggc ttgggacggc attgctgttg cggtttcgga cgcggcttta
      421 ttcgacgacg attttgggga agcggctgct gaagtctttg cctttgtcgg caatgctcag
      481 ggcgatttgg aatgcggcat cggtgtagat tcgggcgatg gcggggtgtt cgtcgaacag
      541 ttgcgccggt gtgccgccgt ccatggcttc gcgcacgggc aggcttaggg gaagctgtcc
      601 gagcaggggg acgttgaggc gtgcggcgaa atctttgccg ccgtccgtgc cgaacagtgc
      661 ttcgctgtgt ccgcagttgg tgcagatgtg gacggacata ttttccaata cgcccaaaat
      721 gggaatgttg actttgcgga acatatccac ggctttgcgc gcgtctatca gggcgatgtc
      781 ctgcggcgtg gttacgatga cggaaccggt tacggggatg cgctgggaca gcgtgagctg
      841 gatgtcgccc gtgccggggg gcaggtcgat aaacaggtag tccacttcgt cccactcgct
      901 ttggaacatc agctgctgca aggcttggct gaccatcggc ccgcgccaga cgacggcttg
      961 gtcggtatcg acgagaaagc cgatagacat gacctgtatg ccgtctgaag attcgacggg
     1021 aatgagtttt tggtttttct gatcgggttt gcggtcgtcc acacccaaca tggtcggttg
     1081 gctcgggccg taaaggtcgg catcgagcac gccgacgcgc gcgcccatgc gcgccattgc
     1141 ggcggcaagg ttggcggtgg ttgtcgattt gcccacgccg ccttttcccg atgcgacggc
     1201 gatgatgttt ttcacgcctt tgatggtggt aacgccgggc tggactttgt gtgtgccgat
     1261 ttcagtgtcc atggacagat ggatgtgtgt gtcgcctgtt tcgggcatca ggatttcctg
     1321 tatgcggtcg gctgtttctg aggcaatgtg cgcgacgggg aagccgaaat gcagggcgat
     1381 atggatgccg tctgaacgct gttcgaccga acggacggcc ttttcgccgc cgagcgtgcg
     1441 tgccgtattc ggaacggcga cggtgtcgag gagggtgcgg atgttttgta tgttcataat
     1501 gtgtgtttct gtgtgcaggt tgggaaggga aacgggcgga aagtgtacat tgatttcggg
     1561 cggtttgctt ttccttgtgc gaacttttac acggtatgcg gcggattgtg aaaatccgtt
     1621 gtcaaaatat cccgtgcaaa cagtggtctg caattaagcc tgtaaaattt gcttgacgag
     1681 gtaggatggt gctgtataat gcacggctta tcgggtcgtt agctcagtcg gtagagcagc
     1741 ggacttttaa tccgttggtc gagcgttcga atcgctcacg acccaccaga taaccggaag
     1801 ccaagttttg agacttggct ttttgtttgc ctgccggttg cgcgggcgta ccgtttgaga
     1861 gagcgtatga aaaagaaact gctttcgggc atcaaatttg ccgttcagac ggcattggtg
     1921 tttttattgg tgtcgctgtt tttggattgg atacgcaagc ccgaagagcc ggccggtgcg
     1981 gcagggcggc ctttgaccct gctgtcgggg cagcggctga ctttgggaca gtttagccga
     2041 gataaggcgg ttttggtgta tttttggggg agctggtgcg gcgtgtgccg ttatcagtcg
     2101 ccgataatcg atgatttggc ggcggacggc gtgccggtcg tcggcgtggc ggtacgttcc
     2161 ggcagttcgg cggaagtggc ggcgtatatg gcaaaacgcg gcttgggctt tccgactgtc
     2221 aacgatgagg acgggggttt ggcaaggtct tggcggattg tcgcaacgcc tgccgtcgtt
     2281 ttggtcaaaa atgggaaaat ggttcactat acgacgggaa tcagcagtta ttggggcttg
     2341 cgcgcacgaa tttttcaggc ggatgttttc ggttaacttt tgtttttatt aaattttccg
     2401 ctatgtagag aatcaagcgg catatgcctt gcgcggcgga tgcggcggct tttgtttttt
     2461 gggaaagagc cgacgcgcct gccgatgtaa tcccttgttg tgattgtggg aaaaatagat
     2521 taagatataa ctattaaaat atttttagat aggattatcg gaattaaagt cttttatacc
     2581 cagtcgtccg atgcggttta tagcgtattg ttgctatatg ttcgttttgt tatataacgg
     2641 ttgcatcaaa atttacgccc acaggctttc ccgacggttt gaaagtttga ttttcgataa
     2701 cttggagact taaacaatgc ctacccaatc aaaacatgcg tctatcaata tcggtctgat
     2761 acaggcaagg gaagccctga tgacccaatt caggcctatt ctgaatcagg cgaatattac
     2821 cgatcagcaa tggcggatta tccgtctttt ggcggaaaac ggcacgctgg actttcaaga
     2881 tttggcgaat caggcgtgca ttttgcgccc cagcctgacc ggtatcctga cccgccttga
     2941 aaaagcgggt ttggttgtcc gcctgaaacc ttccaacgac caacgacgtg tttttctgaa
     3001 gctgactgcc gagggcgaga agctgtatga ggaaatcggc gaagaagtgg acgaacgcta
     3061 cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg ctgcttaaag acctgttggc
     3121 agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac gccgtaacgc gcggaaacgt
     3181 ccgaccgacg gctttttgaa tcaaaactgc tgcacatggg ggatgccttg tgtgcagcat
     3241 tcttatatag gggacagttt aaaggggaaa aatggcggat ttgcagaaaa attttcaaac
     3301 ttcgttccgt gatgcgatgg catcttgcgc ggcaggcgtt catgtcatca cgacagacgg
     3361 tgcggcaggg cgttacggca ttacaatgac ggcggtcgcg ccggttaccg acgagccgcc
     3421 gaccgtgatg ctgtgcatca accggagtgc gcgaatcatt ccgatcctgt cggaaaacgg
     3481 cagcctctgc atcaatacgc tggcggacga acatcaggat gttgccgaac attttgccgg
     3541 gctgaccggc ctgtcgcccg aagagcggtt tgcctaccac atctggcatc gcggcaaaac
     3601 gggacaactt gaaatagagg gcgcgttggc gcacctgcac gggcatattg tcggcaaaca
     3661 tgaaatcggc acgcattttg tgttttacgt caggctcgac gaaatcaaaa actgcgggtg
     3721 caaacgcccc gcgctgctgt atttcagacg gcagtttaga tttttagact gatattcgga
     3781 cagatatatg aaagcgatga tactggcggc aggacgcggc gagcgtatgc gccctttgac
     3841 cgataccact ccgaagccgc tgctcgatgt ggcgggtaag cctctaatcg gttggcacct
     3901 atgccgtctg aagcaggcgg ggtttaccga aatcgtcatc aaccacgctt ggctgggtcg
     3961 gcagatagaa gatgctttgg gcgacggctc ggcttatggc gtgaacatcg cctattcgcc
     4021 cgaacccgca ggcggtttgg aaacggcagg cggcatcgcg caggcattgc cgctgttggg
     4081 tgggcagccg tttttggtgg tcaacggcga cgtgctgacc gacatcgatt ttaccgccgc
     4141 gtttcagacg gcatcgtccc tgccggaaca tatttccgcc catctgtggc tggtggaaaa
     4201 tccgccgcac aaccccgacg gcgatttttc cctgctgccc gacagcagcg tgcggccgga
     4261 agtaaatggc ggcaacggat tgacattcag cggcgtgggt atttaccgtc ctgaaatgtt
     4321 tgacggaatc gaagcgggca gtgtggcgaa actcgcgccc gtattgcgtg gcgaaatgcg
     4381 gcaaaaccgc gtgagcggtc agaagcatac gggcttgtgg ctggatgtcg gcacggtatg
     4441 ccgtctgaaa gaggctcaag cccttgcagg ggcttggaag taaaaacccg gtttcagacg
     4501 gtatggcgga ttcggtttaa cgtttcaacg ccagcaccaa cacgcccgcc gt
//
LOCUS       DAWISC010000154         4506 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWISC010000154 DAWISC010000000
VERSION     DAWISC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.154"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(103..1041)
                     /locus_tag="WIW87_001739"
     CDS             complement(103..1041)
                     /locus_tag="WIW87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5174864.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1050..1925)
                     /locus_tag="WIW87_001740"
     CDS             complement(1050..1925)
                     /locus_tag="WIW87_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ5174865.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWVERVKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLALGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPNK
                     LDNTAAGGVSGGEMPSEAVCRESSEEAGLDKTLLPLIRPVSQLHSLRSVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2121..2197)
                     /locus_tag="WIW87_001741"
     tRNA            complement(2121..2197)
                     /locus_tag="WIW87_001741"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Met,seq:cat)
     regulatory      2347..2446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2539..4440
                     /gene="thiC"
                     /locus_tag="WIW87_001742"
     CDS             2539..4440
                     /gene="thiC"
                     /locus_tag="WIW87_001742"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ5174866.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGICFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLEQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1090 a         1296 c         1187 g          933 t
ORIGIN      
        1 gtttcggata aaattttgca gccctgataa aaaaatatgg ctgctttggt aaaaaaatgc
       61 cgtctgaaag gttttcagac ggcattttgt ttttaagaag catcagcgga agcggacgat
      121 ttcccgttct tcgatatgga tgcgtaccgt atccttgccc gataccgccc cggcgtgccg
      181 catatcgagg ttcagccaca ggatgccgtg ttccggatgg aggacggaca ggctgaacga
      241 ttcgggcaaa caggtacggg ataatacgcg gcactccatg ccgtcttggt cgaaacgcac
      301 cgcatgttgc ggaatatggc ggttatcgtc ggtattgggc aaacccatca gtcgggcgac
      361 ctgcacgcag gatggtgttt tgaccaatgt ttcgggcgta ccgtattgta gaatcctccc
      421 tttatgcatc acggcgattt cgtctgccgt cgtacaggct tcttcgggcg aatgcgttac
      481 caaaacggca gggatgccgc cgtttcggat acgttcggca gtcatacggc gcagcgtgcc
      541 gcgcaaatgc gtgtccaaac tggaaaacga ttcgtccaac agcagcaggg aagggcggac
      601 aaccaaagcg cgcgccaacg ccagccgttg cttctcgcct ccggaaagtt tttcaggctt
      661 gcggtgcgcc tcgttttcca gtccgacttc ggcaagtgcc gccatggcga ggcgttcggc
      721 ttcggctttc ggcatttttt gcattttcaa accgaatgcc gcattttcca gcgcactcat
      781 atggggaaac agcgcgtaat cttgaaacat cagcgagata cggcgttttt cgggcggcat
      841 acgggtaatg ttttctccgt tcagccatat ttccccgccg tccggccgga caatccccgc
      901 aattatattc agcagggtgg attttccgca gcccgaccgc cccaaaacgg cgagtatttt
      961 gccgcgcccg acagtcaggc agatgttgtc ggcgacggtt ttattgccga agcgtttgca
     1021 gagtccgttc agttcaagca tggcgcatcc tataaacgta tgccgtccag ccactcggac
     1081 agcggatggg cggcatcaat cagaccgtaa cggcaaaacg cgtccagcgt aaccagttgc
     1141 gcgtcgtgca tcatgtttcc cgacaacatg gcatccaaca gaccgccgat gtccattttc
     1201 tcaaaacccg ccacttcgcc atcctgattt tcaggcagga aggtttcggg caggacggca
     1261 tcgaatacat acaggatttc attgtgtaca ccccggctga cggagcgcag gctgtgcagc
     1321 tgcgataccg ggcggatgag cggaagcagc gttttatcca aaccggcttc ttcgctgctt
     1381 tcgcgacaca cggcttcaga cggcatttcg ccgccggaaa caccgccggc ggcagtattg
     1441 tcgagtttgt tgggatcgac tgctttgtgc ggactgcgcc tgcctatcca gaaatgccat
     1501 cggccgtccg attcggtcag accgttgaga tggacggcgc ggctgagcag tccgaaagga
     1561 cggaaagcgg cgcgttcgag cgtgaacaag gggttgccgc cgccgtcggt caggtcgaaa
     1621 cactcgttgc gccagccgtc caacagcccc gcacagtgcc aaccgagggc gaggtgctgt
     1681 aagcgtccgc ccatatcagg ccagccgtcc gcattcagaa aaatgccgtc tgaagactcc
     1741 gagcagcctg cctcccagtc ttttttgacg cgttccaccc attccggcga caggttgccc
     1801 aaaggcagac cgttcagata cagcgttttc cagcaacttt ctgcaccgta acttgctttt
     1861 gcccactcga acagagcatc aaggtcttgt ttgctgacgg attcggtaaa acggacggtc
     1921 ggcatggttg gctttcttga agacaatgcg gaagattgta gccaaactgc ctgaatttgc
     1981 aaccgcaaac agacggcggt ctgccgttcc gatattgcgg ccggaaggaa aatccggcaa
     2041 aaaacggagg cggcaggaaa caggaaacag gaaacaggca aaacaaaacc cctcggcatt
     2101 gaaccgaggg gtttgatatt tggttgcggg agcaggattc gaacctacga ccttcgggtt
     2161 atgagcccga cgagctacca tgctgctcca tcccgcgtca gaagatgaaa ctatacggca
     2221 gattttttat gttgtcaaac attacttccg cgaaaaatca taaaattttg ccgtccggcg
     2281 gtttatgcct gatttgaaat attattttct ttacaaaagt tcatgttcgt gatttaattt
     2341 tggttaacat tgaaacaggg gtgctgcctg atgtttaggc ggctgagaaa taccctttac
     2401 acccgatcgg gataatacct gcgtggggag ttttcacgga ttctgctttt cagacggcat
     2461 tggttttcaa atgccgtctg aaaacgcaaa acgctcctgt ttctttaatt ctaaacgaga
     2521 aaacaggagc atttttttat gactacgcca aaaaaaaccg ccaaaacttc cggcaacgaa
     2581 gcgcgcgagc ttgccgactt gagcgaagac atcggcatct gctttaaata tccgaactcg
     2641 gaacgcgtgt atctgcaagg cagccgcgac gacatccgcg tgcctttgcg cgaaatccgt
     2701 caggacgaca cctacacggc gcaaggtacg gaagccaatc cgccgattcc cgtctacgac
     2761 accagcggcg tgtacggcga cccggcggcg catatcgacc tgaaacaagg tctgccgcac
     2821 atccgcaccg cgtggctgga cgaacgcggc gataccgaaa tcctgcccaa gctctccagc
     2881 gaatacggca tcgaacgcgc acacgatccg aaaaccgccc atctgcgttt caaccaaatc
     2941 acccgcccgc gccgcgcgaa aagcggcagc aacgtaaccc agcttcacta cgcgcgccaa
     3001 ggcattatca cgcccgaaat ggagtttgtc gccatacgcg aacgtttaaa attagacgaa
     3061 ttgtcccaaa aaccggaata cgccaaactc ttggaacagc acgcggggca aagtttcggt
     3121 gcgaacatcc cgacccatcc cgaccaaatc acgcccgaat tcgtgcgcca agaaatcgcc
     3181 gccggacgcg cgattattcc cgccaacatc aaccaccccg aactcgaacc gatgattatc
     3241 ggccgcaact ttcgtgtcaa aatcaacggc aacttgggca actccgccgt cacctccagc
     3301 ctgaccgaag aagtcgaaaa aatggtgtgg tcgctgcgtt ggggcgcgga cacgattatg
     3361 gatttgtcca ccggcgcgca catccatgaa acgcgcgaat ggattatccg caacgcgccc
     3421 gtccccatcg gcaccgtgcc gatttaccaa gcgttggaaa aaaccggcgg catcgccgaa
     3481 gatttgactt gggatttgtt ccgcgacacc ctcatcgaac aggcggagca aggcgtggac
     3541 tatttcacca tacacgcggg cgtgttgctg cgttatgtgc cgatgaccgc caaccgcctc
     3601 accggcatcg tctcgcgcgg cggttcgatt atggcgaaat ggtgtttggc acatcatcgg
     3661 gaaaatttcc tctacacgca tttcgacgaa atctgcgaaa ttatgaaagc gtatgacgta
     3721 tcgttcagcc tcggcgacgg cctgcgcccc ggctgcattg ccgatgccaa cgacgaatcc
     3781 caattcgccg aactgcacac cttgggcgaa ttgaccgata aagcgtggaa acatgacgta
     3841 caagtcatga tcgaaggccc cggccatgtg ccgctgcaac gcgtcaaaga aaacatgacc
     3901 gaagagctgc aacactgctt tgaagcacct ttttacacgc tcggcccgct cgttaccgac
     3961 atcgcacccg gctacgacca catcacctcg ggcataggcg cggccaatat cggctggtac
     4021 ggcacggcga tgctttgtta cgttaccccg aaagagcatt tggggctgcc cgacaaagaa
     4081 gacgtgcgca ccggcatcat cacctacaaa ctcgccgccc acgccgccga tctcgccaaa
     4141 ggctggccgg gcgcacaatt acgtgacaac gccctgagca aagcgcgttt cgagttccgc
     4201 tggcgcgacc aatttcgctt aagcctcgac cctgaacgtg ccgagagctt ccacgacgaa
     4261 accctgcctg ccgaaggcgc gaaaatcgcc cacttctgct cgatgtgcgg ccccaaattc
     4321 tgctcgatga aaatcacgca ggaagtgcgc gactacgccg acaagcaaaa agcccaacgg
     4381 cagggcatgg aggaaaaagc ggtcgagttc gtcaaaaaag gcgcgaagat ttacagttaa
     4441 acgtcaagca aaaaatgccg tctgaaaacc ggaaaaaagg cttcagacgg cattctttcg
     4501 cttgtg
//
LOCUS       DAWISC010000155         4364 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWISC010000155 DAWISC010000000
VERSION     DAWISC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.155"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(69..2045)
                     /locus_tag="WIW87_001743"
     CDS             complement(69..2045)
                     /locus_tag="WIW87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ5174867.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WIW87_001744"
     CDS             2194..3033
                     /locus_tag="WIW87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ5174868.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPFVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPTFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WIW87_001745"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WIW87_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ5174869.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAAHDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1111 a         1457 c         1013 g          783 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca ttgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaat gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaatcacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc tttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accgacgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccagtcgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccatc tgaccaaatt cgacaccgta cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 accagcaaag aaaaagccaa agttcatctg gaaagcggcg caaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcac gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcactc aaaggcgttt tgggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacaccacc gaagccagcc acttcgacgc aacgctgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggattc
     4201 agctgtcaaa tgctgaacac tgcacgccgc atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc aatt
//
LOCUS       DAWISC010000156         4357 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWISC010000156 DAWISC010000000
VERSION     DAWISC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.156"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            123..1973
                     /locus_tag="WIW87_001746"
     CDS             123..1973
                     /locus_tag="WIW87_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ5174870.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAKKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            2262..2474
                     /locus_tag="WIW87_001747"
     CDS             2262..2474
                     /locus_tag="WIW87_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ5174871.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2606..3052
                     /locus_tag="WIW87_001748"
     CDS             2606..3052
                     /locus_tag="WIW87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ5174872.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            3073..3222
                     /locus_tag="WIW87_001749"
     CDS             3073..3222
                     /locus_tag="WIW87_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174873.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(3251..3643)
                     /locus_tag="WIW87_001750"
     CDS             complement(3251..3643)
                     /locus_tag="WIW87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ5174874.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3715..4320)
                     /locus_tag="WIW87_001751"
     CDS             complement(3715..4320)
                     /locus_tag="WIW87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ5174875.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1061 a         1230 c         1203 g          863 t
ORIGIN      
        1 atgtcggaaa cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt
       61 cagacggcat tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc
      121 cgatgtacct accctctatg aagcattccc tgccgctgct ggcggccctg gtgcttgccg
      181 cgtgttcttc gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaaactg
      241 ccgacctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaggaaaa acgctggcag
      301 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      361 ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc
      421 tgaagtcttt gggcgcacgc aaacagtgga cgctgtttgc acaggaatat gccaaactcg
      481 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
      541 cgcgtgccgc cgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      601 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
      661 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
      721 cgtttgacgg cggtacacaa ggttcgcgcg aatacgccct gttgaacgtc atcggcaaag
      781 aagcacgcaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      841 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa aacctcaatg
      901 tgcctgccgc cttggactat tacggcaaag ttgccgaccg ccgccaactg accgacgacc
      961 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcttccg
     1021 taatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1081 gcagtcgcgc cgcaacgggc aacacgcaag aggcgaaaaa actttacaaa caggcagcag
     1141 cgacgggcag gaatttttat gcggtgctgg cgggggaaga gttggggcgc aaaatcgata
     1201 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtcct ccgtatggcg gaagacggtg
     1261 cagtcaaacg cgcactggta ctgttccaaa acagccaatc tgccggtgat gcaaaaatgc
     1321 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
     1381 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1441 aacgcaccga ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg
     1501 taatccgcca cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc
     1561 aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
     1621 ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
     1681 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1741 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
     1801 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
     1861 aaacgcgcga ctatgtcaaa aaagtgatgg ccaatgccgc ctactacgcc gccctcttcg
     1921 gcgcgccgca catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg
     1981 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
     2041 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
     2101 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2161 gcaaggcaca gcgatacggt aaactttcaa caccgtctgc cctacccttt ccaccgatat
     2221 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2281 aagagaatga accatttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2341 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2401 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
     2461 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2521 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
     2581 catccgacat cccagcgagg acatcatgag cctgaaaatc cgccttaccg aagacatgaa
     2641 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2701 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2761 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
     2821 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
     2881 aatgctttcc gccggcgaaa tccgtaccga ggtcgaagct gccgttgccg aaaccggcgc
     2941 ggcaggtatg gcggatatgg gtaaagtcat ggggctgctg aaaacccgcc tcgcaggtaa
     3001 agccgacatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
     3061 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatatc
     3121 ccaatatcga atcggcaggg gcaacacggt tttgatacgc cgaaacgggt tttgccgata
     3181 aacagattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
     3241 cggcatagat ttatttgacc aatttcaagc cttttttggc gggtcggggc gcggtttcgg
     3301 cagaggtgtt ttcaggcggc gtatcggggc ggtacgcttc caactcaaac cccatacctt
     3361 ctccggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
     3421 cctgtccgcc gaagcgggcg gaaaagctga tccaatcgtt gtcgatttga aggttttgcg
     3481 tggcggtcgc gccgatgttg agcataattt cgttgtcgcg gacgtactgc atggggacgc
     3541 gcgtgtgttc gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
     3601 cgcagagggc gcggaggatg tagggtttgg tggaagtggg cataatgggt tccgtgttgt
     3661 acgccaaaat aggaaaatgc ctgcaaaacg gtgggttttg caagcatttc ggacttattt
     3721 gcgcatggct ttttcggcgg gtgtcagtgc ttcgataaag gcttcgcgct ggaagatgcg
     3781 ctcggcgtat ttgagcagcg gcgcggcact tttgcccagt ttgacatcgt agtggtcgag
     3841 ccgccacagc agcggagcaa gggcgacatc aatcatagaa aaatcttcgc cgaggatgta
     3901 tttgcttttg ctgaacgaag gggcaagcat ggtcagaccg ttgccgatgg cttcgcgcgc
     3961 ttttgcctgt tccttgttgg tggcggcggg gttttctaac acttggacgt ggttgaacaa
     4021 ttctttttcc atacggtaca gcaccagccg gccccgaccg cgcataacgg gatcgccggg
     4081 catcagctgc ggatggggga agcgttcgtc aatgtattcg ttgatgatat tggactcgtg
     4141 cagcaccaaa tcgcgctcga ccagcacggg aacttggtta tacggattca tgacggcgag
     4201 gtcttcgggt ttgttgtaaa tatcgacgtc tttgatttca aaatccatac ctttttcgta
     4261 caaaacgaag cggcagcggt ggctgaaggg gcaggtaatg ccggaataga gggtcatcat
     4321 aataattgtc gctcctgtgt gatgcctgca aaacggc
//
LOCUS       DAWISC010000157         4340 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWISC010000157 DAWISC010000000
VERSION     DAWISC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.157"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..2284)
                     /locus_tag="WIW87_001752"
                     /pseudo
     CDS             complement(1..2284)
                     /locus_tag="WIW87_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            complement(2841..3290)
                     /locus_tag="WIW87_001753"
     CDS             complement(2841..3290)
                     /locus_tag="WIW87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174876.1"
                     /translation="MQPGSKNDEKNKITLNEFNNAIVTIFSDENVVRAIGTINTMHGE
                     YLTRSHKIDQQKFLSDGERLSSKAKDFQHSAEKTQRALKEGIGRAASKFHANSKEAFV
                     ARKMQSDLQKKLNASNYNKQQYARQAVTALENASKAENKGNIAFYLN"
BASE COUNT         1345 a          924 c          716 g         1355 t
ORIGIN      
        1 gcgtagtcgt aattatagac cgtatcctga ccgaagcctt cgccgaagac ataagtatcc
       61 gaaccgctgc cgccctccaa atagtcatta ccggcgccgc cgattagagt gtcgttgccg
      121 tcattaccat ataaagaaac atttttatta tgaccaaagc ctacattttg caggatatca
      181 tctgcttgcg tacccgatgt tttagctaat aatgcaacgg tctcctgacc caacactttc
      241 tggtaatctt caaatttacc tgcttttttt gcctcctcca cataatcggt cattagtctt
      301 cggccttcat accaagaacg aagttcgcca tatgcaagca tctcggccaa atccacaaaa
      361 gctttttgcg gattagtttc tttgacatgg ttaaatgctt gaacaagacc actaaaatcc
      421 aaagtgaacg tatcattttc cattttgaaa ctgatttgat tcaaatatgg ctgcaaacgg
      481 gtttggaaca acaggttttg gtagatgttt ttggcgagat ggtcgtatgt atcgttggtt
      541 actttgacga tattaagcgc atcttcctcg ctcatgtaat agagtgtgtt ggaatcctgc
      601 cccgtgtagg catcaagcac ggcaatgcgg tcgcgggcgg cgtcaatagc tgctttagct
      661 ttatcagaaa gggaaactaa agcgttcttt tttagttgtg ctacttggga tggtgtcagt
      721 gcaatacctt cattagccgt ttgcgtccaa tcggttgaaa gtcgcattgg cgattttttg
      781 ccccagttcg aatcggtttc cgcccatttg tgaatcaaat tatctaacaa tgccaactgt
      841 gcttctttag tttcggcggc agaataagct ttcagcatat tggccaaatc gccggacaat
      901 gcggcagctt cgcgcaaatc gcgcagacga ccaatgcccg caagattggc ggcttttgcc
      961 tgttcggcag tgagttccac tttgtctttg aagcggctgt gcagattgtc ggctgctaaa
     1021 agtaaatccc ccatttttgc ggttgtaccg tctgttttgg tatagctgcc ttgctgagcc
     1081 aaagtgttac cgttaccgag atttttattt acatctttat aggcgagatc caaagattgg
     1141 atacccaatt cttcaagggt acgcaattca ttagcttggg aaatgccgtc ctggttgaga
     1201 tcctgccata cacgcaggga ttggaatgcg gcgtctgccg cgttgatgat gttgtcgccg
     1261 tttgaatcca attcggccaa agccgcgtag ccgtgtttgg caaaagagcc gtctgccagt
     1321 ttggtgttgt cgccgaagag ttccgcaccg ttgtcgatga tgccccgttc aaatcgcgca
     1381 caagcagacc gtcatcggca gcaacccaac ctgtggcggt gcggatgcct tgattgcggt
     1441 ggtcgaacaa tgcacctgca aagcctttgg tggcaacggt ttctataccg tcgccgtcta
     1501 gatctagggc gagggggtcg taaacgtggt atttgtcgct gcggttaaga tttaagaaat
     1561 cctcaaacca tctccaagct ttttcagctc ctaatgatcc ccaccaagct cctgcgccac
     1621 ctccaagtat agctccaact actagccaac ctgcgccacc agtaagtaaa ccaaaagccc
     1681 caacacccaa agccgcaccc cattggacgc ccattagccc acccaaaaaa gaagctgatg
     1741 agagaccaac atcactccaa tttccttctc catccaaggc tttccaccaa gcacttccca
     1801 tactcaaagt tcccatggca acagttgccc cataagtaaa cttacccaaa ttcgctgcca
     1861 taacttttga ttttgaagca ttctctaacg ctgttgccgc acgtcttgca tattgctgct
     1921 tgttatagtt gctagcattc aatgcttttt gcaaatcact ttgtactttt cttgcaacaa
     1981 atgcctcctt tgaattacca cgaaatttac tagcagctct tccaattcct tcttttaagc
     2041 ttttttgtgc ctcttctgct gtgcgttgga attctcttgc cttagcagac agacgctcac
     2101 cctcccttaa aaattctttt tgatctatct tgtgcgaacg ggtcaaatat tcttcgtaca
     2161 ttgagttaat cgtaccaatc gccctaacca cattttcatc agaaaatatg gaaataatag
     2221 catccctata cgcacctagt gtaatattgt ttctattatt agttatagca ttattcgaat
     2281 acataatagc acctccaaat ttagaactaa atacgtttat attctctgtg taataatatc
     2341 aaataattaa ttaatggata agtaagtccg aaaaaataac aactaaaata agcaatatac
     2401 tgattctctc ctttattaat tagatatatt gaaaaacaaa taaaaaataa acagaaaaca
     2461 ttcatgtgaa aaagccacct catattctta tttataagtt taaaataact aggcattgta
     2521 tatttaaaaa tcggcacatc caacggaaac aaaccacata aaaatttttc tttattcttt
     2581 attaaagaca tagttttatt ttttaaaatt agtccaaata atatactaac aattactatt
     2641 aaaacaataa gcacagcgta attttttata aaattcatta aaataataac gaaaatacta
     2701 aatagattac atactataaa ttgaaatata aaatattcag tatttaatcc attttcaacc
     2761 gcgagttttt taaaatatcc ttgctcgaaa aaattaaaaa aatccattaa tactttcctc
     2821 tcaagaaaac taattttagt ttagttaaga taaaatgcaa tattaccttt attttctgct
     2881 tttgaagcat tttccaaagc tgtgactgct tgtcttgcat attgttgctt gttatagtta
     2941 ctagcattta acttcttttg caaatcactt tgcatttttc ttgcaacaaa tgcctccttc
     3001 gaattggcat gaaatttact agcagccctt ccaatccctt cttttaaagc cctttgagtt
     3061 ttttctgctg agtgctgaaa atctttcgct ttactagaca accgctcacc atcactcaaa
     3121 aatttttgtt gatctatttt atgcgaacga gttaaatact ccccatgcat tgtgttaatt
     3181 gtaccgattg ccctaaccac attttcatca gagaaaatag ttacaatggc attattgaat
     3241 tcatttagtg taatcttatt tttttcatca ttcttagaac cgggttgcat gacaacacct
     3301 tccatcaact aaaatattat attattaaga gactacaact tagatacgtt tataatctct
     3361 ggataaaaaa acgtaataag tgtttgctgg ataacaaagt ccaaaccaat agcagataaa
     3421 ataaggcatc cacccccttt cttcattaag gatcagagat atcgagaaac aaatcataac
     3481 taaacagaaa aaattttgga gataaagcca tttcattccc ctattcaaga atctggccaa
     3541 gataggtatt ttgtatactc tagaaaagaa aggcatttcc aagggaaaca tatcagataa
     3601 aaacttttgt ttatttttta ctatagatag aaccctattt ctcaagataa atccaaatgc
     3661 aatgccaatt atcattatta ataaacaaag gatagtataa ctatggataa ccttcattaa
     3721 acaaaaagaa gaaatggcag ataaattaca tacgatatag tggaatataa aatatttacg
     3781 gtctaaacct tgttcagttg caattttttt atacttacct tgcgcaaaaa aaatcaaaga
     3841 aatccattaa actatctttc acattagaaa tttaaaagct aaataatacg acaaacaatg
     3901 tgaagtacta ttcatggttt atttaaaaaa taatactatt ctgaacatta tttagataca
     3961 gaaattaaca aattggaact aaacaagctt ttaaatactt taattttatt ggaaagctat
     4021 aaaaggaact atagtttcac acactaatta cttcctaatc acttctttat taagaggcac
     4081 aagggattgg ggaggctgtc ttggagataa gcactgatgt tctaggcaaa cccctccatc
     4141 ttaaagttaa aacaaccaca ggcagcctgg aaatgatcta tgatctatgc ccatacaagg
     4201 acattctaga tagttttcct tgttgcttcg tgtatcacac aagatagaat tggcactcta
     4261 ctctttcctt aaaaaaccgt caagaatggt tatcaatttt tttaaactta ttcaaaacaa
     4321 tattcttata cacatctcaa
//
LOCUS       DAWISC010000158         4311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWISC010000158 DAWISC010000000
VERSION     DAWISC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.158"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            240..1160
                     /gene="ribF"
                     /locus_tag="WIW87_001754"
     CDS             240..1160
                     /gene="ribF"
                     /locus_tag="WIW87_001754"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ5174877.1"
                     /translation="MRIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGHTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQGLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAARRW"
     gene            1302..4091
                     /gene="ileS"
                     /locus_tag="WIW87_001755"
     CDS             1302..4091
                     /gene="ileS"
                     /locus_tag="WIW87_001755"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ5174878.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYLFKDTAA
                     LAAAFGLAGFEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQECLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1057 a         1181 c         1142 g          931 t
ORIGIN      
        1 ttgtctcggt ttacctggtt aaaaaagaac gattttcact gatgttgcat caggtttggg
       61 gcgatgtttc aacacatagc accgcgcctg ctgcgcgttt ttgtgcgttt ggcgcgttcg
      121 gcggcgggaa atttgcctac ttttcccgcg tcgggcgggc gtaacgggcg gcacactgtc
      181 tataaaccgc aataccgttt acaatgaccg cctgtttcac cacatacccg aatgcaacaa
      241 tgagaatcag gctggggcgg cacaacgcgc ccgactttcc acagggtgcc gccgtaacca
      301 taggcaattt cgacggcgta cacctcggac acaaacacat cctccaaaaa ctccgcctcg
      361 aagccgacgc gcgcggactg cccgtcgtga ccgtcgtttt cgaaccccaa cccaaagaat
      421 ttttcgcact ccgcaccggc aggatgccac cgtgtcggat cagccccctg cgcaccaagc
      481 tcgaattatt ggaaggcaca ggctgtgtcg atgccgtctg ggttttgcgt ttcgatcaaa
      541 atttttccga aatatccgcg caagggttta tcgaccgcct gctgcgtcaa accttgaata
      601 cgcgttattt gctcgtcggc gatgatttcc gtttcggtgc ggggcgggaa ggctgttttg
      661 aacttttggc acaacagccc gatatgcaga ccgagcgtac gccttccgtc atcgtcgaag
      721 acatccgcac cagcagtacc gccgtgcgac aagccctttc agacggcaac cttgcctatg
      781 cgaaaaaact tttgggacac gactacgtct tgagcggcag ggtggtgcac ggcagaaaac
      841 tcggacacac cttaaacgcc ccgactgcca acatccgcct gccccgccac cgttatgcac
      901 tcggcggcgt gtttgtcgtc gaagcagacg gcgcattcgg cacgcggcgc ggcgtggcga
      961 gtttcggctt caatcccacc gttgatagcg gctgttctca aaagcttgaa gtccacctgt
     1021 tcgactttca aggcgacctg tacggacaag ggctgaacgt ccgcttcctg cacaaactgc
     1081 gcgatgagga aaagtttgac ggtatggaag aactgaaaag gcagattgaa gccgatatgg
     1141 aagccgcaag gcgttggtag aaaaacctta tacaaaccat ccgattgggc tacaatcaat
     1201 cttttaactg ttcagacggc acagggtttt cccgttgtga aatgctgttt ggggcgcaat
     1261 gccgtctgag accgaaatat tgtaacaata gagattaaaa aatgaccgat tacagtaaaa
     1321 ccgtaaacct gctcgagagc ccgtttccga tgcgcggcaa tcttgccaag cgcgagcctg
     1381 catggctgaa aagctggtac gagcaaaaac gctaccaaaa actgcgcgaa atcgccaaag
     1441 gccgtccgaa atttattctg cacgacggcc cgccgtatgc caacggcgac atccacatcg
     1501 gtcatgccgt caataaaatt ctcaaagaca tcattatccg cagcaaaacc caagccggtt
     1561 ttgacgcgcc ttatgtgccg ggttgggact gccacggcct gcccatcgaa gtgatggtag
     1621 aaaaactgca cggcaaagat atgcccaaag cacgtttccg cgaattgtgc cgcgaatacg
     1681 ccgccgaaca gattgcccgt cagaaaaaag actttatccg cttgggcgtg ttgggcgact
     1741 gggaccatcc ttacctgact atggatttca aaaccgaagc cgataccgtg cgtatgctcg
     1801 gcgaaatcta caaatccggc tatctctacc ggggtgcgaa accggttcaa ttctgcttgg
     1861 actgcggttc ttcgctggcc gaagcggaag tggaatacaa agacaaaatc tcgcccgcga
     1921 ttgacgttgc ctatctgttt aaagacactg ccgcgcttgc cgccgcattc ggtttggctg
     1981 gtttcgaagg caaagcgttt gccgtcattt ggacgactac gccttggacg ctaccggcga
     2041 gccaagccgt gtctgcgggt gcagacgtgg tgtatcaact gattgatacg ccgaaaggca
     2101 aattggtatt ggcgaaagat ttggcagaag acgcgctcaa acgttacggt ttttcagacg
     2161 gcattgctat tctcgccgaa accaccggcg acaagctgga aaatctgcac atgaaccatc
     2221 cgttcctcga acgcgatatt cccatgctca acggcgaaca cgttaccacc gatgccggta
     2281 ccggcttggt acacaccgcc cccgcgcacg gtttggaaga ctacgccgtc tgcaataaat
     2341 acggcatcga gctttacaac cccgtcaacg ccgaaggccg atacatcggc gaaacgccgc
     2401 gtgtcgccgg tatgcgcgtt tgggaggcga accccgtcat cctgcaatgg ttggaagaaa
     2461 ccggcaacct tttggcaagc agtaaaatcg aacacagcta cgcccactgc tggcggcaca
     2521 aaacgccgct gatttaccgc gcgacaggtc aatggtttgt cggtatggac aaagccggtg
     2581 ccgacggcaa aaccctgcgc gacaaagcca tcaaggccgt ggacgacacc gaattcttcc
     2641 cgtcttgggg tcgcgcgcgt ttggaagcca tgattgaagg tcgtcctgac tgggtggttt
     2701 cacgccaacg ctattggggc acgccgatga ctttctttgt tcacaaagaa acgggcgagc
     2761 tgcatccgaa ctctgccgaa cttttggaaa aagttgccct gaaaatcgaa gaaaaaggca
     2821 tcgaagcgtg gttctccctc gataagagcg aactcttgag cgcggaagat tgcgaaaatt
     2881 acgataaact ttctgacaca atggacgtat ggttcgactc cggctcgacc cattattccg
     2941 ttgtgaaaca acgcgaagaa ttggaatggc cggctgattt gtatctcgaa ggcagcgacc
     3001 aacaccgcgg ctggtttcaa tcgtccatgc tgaccggctg cgcctcatca atgggtcgcg
     3061 cgccgtataa acagctgctg acccatggtt tcgttgtcga cggcgaaggc aaaaaaatgt
     3121 cgaaatccat cggcaacgtc gttgcaccgc aagaggttta taacgaattc ggcgcagaca
     3181 tcctgcgcct gtgggcggca tctaccgatt acagcggcga attggcgatt tccaaagaaa
     3241 tcctcaaacg cgtaaccgaa agctaccgcc gtatccgcaa taccttgagc tttttgtttg
     3301 ccaacttgag cgactttaat ccgattgaag atgccgtgca acaggcggat atggtggaaa
     3361 tcgaccgcta cgccgtggta ttggcacgtc agctgcaaga gtgtctggca ggcgattact
     3421 atccgcgtta tgccttccac tttgccgtaa aagacattgt ttctttctgc tcggaagact
     3481 tgggtgcgtt ctacctcgac atcctgaaag accgcctcta caccaccaaa gcagacagcc
     3541 atgcacgccg cagcgcacaa actgccctgt atcacatcac acgcagtttg gttctcttga
     3601 ttgcaccgat tttgtgcttc accggcgaag aagcgtggga catcatcggc ggcggcgaag
     3661 aagacagcgt cctcttccat acttggcacg agttcccgac catcaacgaa aaaaccgaag
     3721 ccgaactggt gaaaaaatgg acggcaatcc gcgaagcccg cgaagcggta accgccgcca
     3781 tcgagccttt gcgcgccgac aaaaccgtcg gttcgtcctt gcaagccgaa gccgaaatta
     3841 ccgcgccgga agaaatggcc ggctatctga atgctttggg cgaagaattg cgctttgctt
     3901 tgctggtgtc taaagcagaa gtgaaagtag gcagcgaact tgccgttgcc gctaaagcca
     3961 gtgatggtga aaaatgcgaa cgctgctggc actacacccg cgatgtgggc gcggttgcag
     4021 gctatgaaac cgtctgcaaa cgctgtgcag agaatgtcgg cggagaaggc gaaacgcgcc
     4081 attacgcctg ataaagtttg agcaaatgcc gtctgaaacc gccgaacagc atttcagacg
     4141 gcattttttg tgccgcgatt tgtctttata acggcggagg ggtttcaaga ttgcggtgtt
     4201 gtcggaatgc aactgaaccg tcattcccac gaaagtggga atctagaatc tcgaggtttc
     4261 agtcatttcc gatagattcc cgcctgtgcg ggaatgacgg atttcgagat t
//
LOCUS       DAWISC010000159         4301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWISC010000159 DAWISC010000000
VERSION     DAWISC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.159"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            579..2306
                     /gene="ilvB"
                     /locus_tag="WIW87_001756"
     CDS             579..2306
                     /gene="ilvB"
                     /locus_tag="WIW87_001756"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ5174879.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2317..2808
                     /gene="ilvN"
                     /locus_tag="WIW87_001757"
     CDS             2317..2808
                     /gene="ilvN"
                     /locus_tag="WIW87_001757"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ5174880.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2875..3168
                     /locus_tag="WIW87_001758"
     CDS             2875..3168
                     /locus_tag="WIW87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ5174881.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3248..4261
                     /gene="ilvC"
                     /locus_tag="WIW87_001759"
     CDS             3248..4261
                     /gene="ilvC"
                     /locus_tag="WIW87_001759"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ5174882.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1119 a         1031 c         1081 g         1070 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatac aatcaatata ttaagtcgtt
       61 gttagccctc ttggttctgt caatgccgtc caatacagtc cggtgcaatg agccattttc
      121 aggaagtatg gttgggaata tttttgatat atcaaaaata aaatcaagtt caatcaatat
      181 cccgagtctt tattgggatg aaaccctgta aattttacaa ttcttgatat cgaagaaggg
      241 tttttcacat ctgcccctcc tacacaaact gtcaacaatt tccatctttc aaaaaaggta
      301 ctcctgattt tattctttta aaatcaatca ataatttctc tgctttcaaa aaatattaaa
      361 agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt atcgtttcta ctctttttca
      421 caaactctgt gttcctttcc aattgattgg ataatccgtc tgttgccgtg ttcccccaat
      481 gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg caaagcggtt
      541 tgtccctatt ggtttgaaac cgtataaaag aggtcgttat gcagttatca ggcgcgcaaa
      601 tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt tttcggttat cccggcggtg
      661 cggttatcga aatctacgat gcccttttcc aactcaataa attcaaacac attcttaccc
      721 gccatgagca ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc ggtaaggtgg
      781 gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc actgaccggt attgctactg
      841 cctatacgga ttcgattccg atggtggtca tcagcgggca ggtaggcaat tccctgattg
      901 gtacggatgc gttccaagaa gttgatacgg tgggtattac ccgtccgtgc gtcaaacaca
      961 atttcctggt tacggacatc aatgagttgg cggaaaccat taaaaaggcg ttccaaattg
     1021 ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc gaaagatgtt acgcaggcga
     1081 tggcgaaatt cagctatcct caggaagaca tttttatccg ttcgtatcaa ccggttgttc
     1141 aaggacatat cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc aaacgtccgg
     1201 tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg acccgatttg
     1261 tccgaatgac gggtgctccg tgcacgggta cgctgatggg cttgggcgct tatccttccg
     1321 gcgaccgcca attcctcggt atgctcggta tgcacggtac ttacgaggca aaccttgcca
     1381 tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt gtggtttccg
     1441 taccgtccaa atttttcgag aaggcgaaga aggttatcca tatcgatgtc gatccttcca
     1501 gcatcgccaa acgcgtgaag gtggacattc cgattgtcgg cgacgtgaaa aacgttttgt
     1561 ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat gctttgggca
     1621 aatggtggaa aaccatagag gaatggcgtt cccgagattg cttgtggttt gacaacggca
     1681 gcgaaattat caagccacaa tatgtgattc agaagcttgc cgagattacc ggcaattcgg
     1741 caatcatcac atcggatgta gggcagcatc aaatgtttgc ggctcaatat tatcccttcg
     1801 aacgtccgcg ccaatggctc aattccggcg gtttgggtac gatgggcgtg ggtctgcctt
     1861 atgcgattgg tgcaaaactt gccgccccgg atcaagacgt attctgtatc accggcgacg
     1921 gctcgatcca gatgaacatc caagagctgt ccacctgttt ccaatatcgg attccggtta
     1981 acgtcattac gctgaacaac ggttatctcg gtatggtacg ccagtggcag gaaatatatt
     2041 acggcggacg agagtcggaa acctatttcg attctttgcc cgatttcgtc aaacttgccg
     2101 aggcatacgg ccatatcggt atccgcgtgg acaagaagtc tgatgtggaa ggtgcgttgt
     2161 tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc gaccagaaac
     2221 agaatgtgat gcccatggtc ggcaacggca aaggtttgga cgaaatggta cttccgccgc
     2281 atatgcgtgc ggacggaaag gcgtaaggag agacaaatgc gacatatctt atctgttctg
     2341 attgaaaacg aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc acgcgattac
     2401 aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg gatgaccatc
     2461 gttacccacg gcgacgagca ggtcatcgaa caaattacca agcaactcaa taaattgatt
     2521 gaagtgatta aagtggtcga tttgaatgaa agccgttttg tcgaacgcga actgatgttg
     2581 gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt tacgcttgac cgaaatctac
     2641 cggggcagca tcatcgacgt aaccgaccgc agctatacga ttgaaattac cggctcgaca
     2701 gacaagctgg attccttttt ggaaacggtc ggacgcgccc aaattttgga aaccgtacgc
     2761 acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga aaatttaacg ccgtaacctt
     2821 tcagacggca cggtatttga atgtcgtctg aaaaacgaac ggcaggagag atttatgtcg
     2881 aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat acacagaaac actggcagca
     2941 cagtttaaag aactggtcaa agccagccgt gcagaagagg gcaacatcag ctacgatctc
     3001 catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa atctcaagca
     3061 gctattgacg aacataacgc cagcgcgcat ttccaagcct tcgtccagtc cgtcgacggc
     3121 aaaaccgaag cgttggaaat cgtattgatg aatgaagttg ccgattaagc ggcacactct
     3181 gtttaacccg tccgaagccg ctcaacattt taaggcttcg acaaccattt accttaaagg
     3241 aaatcaaatg caagtctatt acgataaaga tgccgatctg tccctaatca aaggcaaaac
     3301 cgttgccatc atcggttacg gttcgcaagg tcatgcccat gccgccaacc tgaaagattc
     3361 gggtgtaaac gtggtgattg gtctgcgcca aggttcttct tggaaaaaag ccgaagcagc
     3421 cggtcatgtc gtcaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg ttatgctgct
     3481 gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa gttacagcca atttgaaaga
     3541 aggcgcaacg ctggcatttg cacacggctt caacgtgcac tacaaccaaa tcgttccgcg
     3601 tgccgacttg gacgtgatta tggttgcccc caaaggtccg ggccataccg tacgcagtga
     3661 atacaaacgc ggcggcggcg tgccttctct gattgccgtt taccaagaca attccggcaa
     3721 agccaaagac atcgccctgt cttatgcggc tgccaacggc ggcaccaaag gcggtgtgat
     3781 tgaaaccact ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag ccgtattgtg
     3841 cggcggcgtg gtcgagttga tcaaggcggg ttttgaaacc ctgaccgaag ccggttacgc
     3901 gcctgaaatg gcttacttcg aatgtctgca cgaaatgaaa ctgatcgttg acctgatttt
     3961 cgaaggcggt attgccaata tgaactactc catttccaac aatgcggagt acggcgaata
     4021 cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca atgccctgaa
     4081 acgcattcaa accggcgaat acgcaaaaat gtttatccaa gagggtaatg tcaactatgc
     4141 gtctatgact gcccgccgcc gtctgaatgc cgaccaccaa gttgaaaaag tcggcgcaca
     4201 actgcgtgcc atgatgcctt ggattactgc caacaaattg gttgaccaag acaaaaactg
     4261 attgttttca aacgggacta cctacacatc gtgtaggcag t
//
LOCUS       DAWISC010000160         4222 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWISC010000160 DAWISC010000000
VERSION     DAWISC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.160"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(163..1566)
                     /locus_tag="WIW87_001760"
     CDS             complement(163..1566)
                     /locus_tag="WIW87_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ5174883.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(1635..3062)
                     /locus_tag="WIW87_001761"
     CDS             complement(1635..3062)
                     /locus_tag="WIW87_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ5174884.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3297..3650
                     /locus_tag="WIW87_001762"
     CDS             3297..3650
                     /locus_tag="WIW87_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174885.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3647..3868
                     /locus_tag="WIW87_001763"
     CDS             3647..3868
                     /locus_tag="WIW87_001763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174886.1"
                     /translation="MKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFSDG
                     EYRYVDVLQPNHSDIIRYSGKDFSAKSNI"
     gene            3939..4184
                     /locus_tag="WIW87_001764"
     CDS             3939..4184
                     /locus_tag="WIW87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174887.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
BASE COUNT          997 a         1121 c         1033 g         1071 t
ORIGIN      
        1 tatacatttg aattaattcg cacttaattt aaatgtgttt ttaactgtgc tttatttaaa
       61 ggcaatgaga atgtgaaagt atcggatcaa tcccaaagca gcctgcactt tcgaaacggg
      121 gtgcaggctg ctttgggaat ttcataaccg tttcagcctg ctttattccg caaataccgt
      181 ttccaaccct aacccgctct ctttcaccaa gcgcaaataa gccagcatga atttataccg
      241 tgcttgagcc agtttctgtt ctgcttgggc gacttcctgc cgcgcccgta ttacttccag
      301 ccggttgcgg atgccgtatt gttggccggt ttcggtcgat ttcagtttca aacggctgct
      361 ttccaaaacc cgttcttgcg ccatgatttg gtaacgcgcc gcaccgcttt cggtataagc
      421 ctggcgtacg gcgagtttga tgtgccgctc ggttgcggtc agctgtgctt cggcggcccc
      481 gtattgcgct tcggcttcat ggattttgcc cgacaattct ccgccggtat aaagcggcaa
      541 attcaactgt acgccgacgc tcatcccttt gccccgatag tggtagtcat tattctgcgc
      601 agatgaagtg tagaggttat tctgatagcc gacatgggca gaaacggtgg gatagcggct
      661 gttctgtgct gcccgaagcg cctgtccgct gctttgcagg gcaagctgct gcatccggta
      721 ttcatgattg ttggataagg caatgcgctg ccattcatcc agactgtaac gttccagctt
      781 gggcagatag cgtgccaaca ggttggcggt atctatggcc tcgatttgtt tgctgtccag
      841 gccggtgtag tcgttcaact ggttttcata ggtttgtttc tcagccaata cggcgatttc
      901 ttgggccagg gcattgtcgt aaccggcttt ggcttcgtga atatccagcg cggtggcagc
      961 acctttattg aataaagcct gcgcctgcct tacctgctgg gcataagcct ctttttccgc
     1021 cgcatgggcg gcaacggtgt ctcggctgag taaaacgttg aaataacttt cggcaacttt
     1081 caacagcaat tcttcgcgtg ccgcatcgaa acgctgttct gcagcctgcg tatcgaacct
     1141 gctttggcgg tattgtgcaa atttggcagc gtcaaataag gtttgtccca cctgcacgct
     1201 ccatccctgt gtttcgcggg tggaagaaat cgatggcggc tggcgctggt agctggcatt
     1261 ggcggataca tggggaagga atgcggcctt ggcttgttgt tgccgtgcgc gcactgcatc
     1321 acgctggtaa tgggacgctt gaaaatcagc cgaatgttgc tgcgccgccc gccatgcttc
     1381 aggcagcgta aaagccgaaa cggatgggga aagggatagt ggcaaggtaa aaagtgaaac
     1441 gggtaggata tatttggaaa aataggattt catagccgaa aatagttcat gttgcaaata
     1501 gggcgtcagt gtcaggcaaa cggaaatacc gtaatcttgc attatcatta gattgagcaa
     1561 tgtcatccgg gcaatggttt caggcagtct gcatgtccga accggcggat aacaaatgcc
     1621 cagtacggat ccgcctatcg ctccctaaag ctttcgtcca atttggtttg cagcgggctt
     1681 aacagataat ccagcacccg ccgtttaccc gttttaatct ccgccgtgac attcatgccc
     1741 gccgtcagat tcactgcttt gccgtcaata ttcaaggtat gtttgtccag cgacaccacc
     1801 gccgtataaa ccaagcccaa ctgttcgtgg cttaccgcat catggctgac acttttcacc
     1861 ttgcccgtca gataaccgta gcgcgtatag ggaaaactct caatcttcac caccgcatcc
     1921 tgtccctgtt ccacaaaacc gatgtctttg ttcaatacca aaacttccac gtccattttg
     1981 tcgtcatcgg gcgcaaccac catcattttt tgggcagcct gcaccacacc gcccaccgta
     2041 taggtggcca attcctgcac cgtgccgtcc gcaggcgact gtattgtcat cagctgctgc
     2101 cgctgctttg ccttatccgt ttggccgcgg tattggtcaa tctgttcgtt tgcctggcgc
     2161 agcgcatcca gcgtatcgcg tttcaggttc tgcgtattca gcacccgact ctgctccgcc
     2221 tgtgcaatgg ccgcctgaat ctgcctcatc tgaccgcgcg tactttccaa atcgttccaa
     2281 ttgctgaccg atttgctctg ctgctccaaa aacgcatgtt ccgaaataaa attgtcggcc
     2341 cgcaaacggc ggtagtctgc tgttttctgc tgctcgatcg cccccaccga aaccagcttc
     2401 tgctcctgcg ccttggccga ctgcaattcc gcctgatggc cgcgcaaagc cgactgcaat
     2461 tgcgcatcct gcgccgccca tgcctgatac tggtgctgcg ccaacacctg cgccgattgc
     2521 acatcggcat cggagagacc taaagaccgt gcttgcgcca tatcgatatg cggcacggta
     2581 cggctttcca atgccgccaa taccgcttca taacgcagtt tggacaattg ggcagcctgc
     2641 aaagcctgct ccgactgcac cacatcgctg tctgttccca cagcctccag ttccgccagc
     2701 gtttctccct gtttcacatg ctgcccgtcg cgcacatgta ccgccttaac caccgccgtt
     2761 tccagcggct ggatggtttt gctgcgcccg cccgacaccg ttttgcccga agccgccgcc
     2821 acaatatcga ttttgccgaa ccaggaccac aacaaagcca aaagcgcaaa cgccataata
     2881 aaacgcgccg cccatttcgg agcggcagag accggcgtat cggtcagttc caaatgcgcg
     2941 ggcaaaaacg cctgttcttc cgccgtgcgt ttgggcggtt tcaactggtc gcgcaccgcc
     3001 caaacattgc gccatacagt aatgtatcga gaaagaaagg atttcagggc ggagaaaaac
     3061 ataacgggta taaccttggc aatatagaaa caggaaacaa tataaatatg taaaggaatt
     3121 ttaacggaaa gcgcggcagc tgttaaggga aggcgggaat attgacaaaa aatacccaag
     3181 tcgttacaaa tattcattat tttactgcgt aacgcaacgc tgaagcgcag gctgcttttg
     3241 agatgcggca aggttcggca aaaagcagcc tgcacattta accacaggaa caacccatgt
     3301 ttaccacaaa cgatttacgc catttcctag aaggtttggc catcctattc tcaatcggct
     3361 attggggcac catgctgctg ttgctttggt ttctcgtccg ctttgcctat aaaaagccca
     3421 aacggaaccc cggcaaaatc ctttcaggca gcctgaccgc cgccgctatc ctgatgctgt
     3481 ttgtttggat tattcccaaa caattcggcc cgatcaaaga agaaatacag gcacaagaag
     3541 agtgggacag aaaatacaaa gaagccgaag ccgtgtttaa cgaacaatgc aaaacggcgg
     3601 gggaaagatt taccagacgg cggacaatgt ggaagggatt atgctgttga aggtagtacc
     3661 tgagcgtacc gtttcggcag atgcaaaaac cagagacccg atgtgggaca atgcggcttt
     3721 acagaccagc gaaggcgtaa attttattgc tcgtttccta ggatttttta gcgatgggga
     3781 ataccgctat gtggatgtcc tgcaacccaa ccattccgat attattcggt attcaggtaa
     3841 agatttttcc gctaaatcaa atatttaatc atatacaccc cgcccgttat gcggtaacgt
     3901 tcgaaaacaa tgtcgattcc aagctgcgca ggcactgggt ggcaggtgcg accatacgga
     3961 ttatcgaccg ccaaactgac gaagtgattg ccaagaaaac catctatgtc tttgaaaaag
     4021 gcttggacgg cacgggtggg gcgagaatgc cgtggaagtt tgctatcttg tgcaataaag
     4081 aaagacttac ttcttcagag ccgttatcgg attttgttct tagcgtttta aaaccttata
     4141 tattgcgtcc cttatatatt gcgtccctaa gaagggacga ttaacaaaaa ttaacgtcct
     4201 ttactttcta caagtaacag gg
//
LOCUS       DAWISC010000161         4214 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWISC010000161 DAWISC010000000
VERSION     DAWISC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.161"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(248..1111)
                     /gene="rpoH"
                     /locus_tag="WIW87_001765"
     CDS             complement(248..1111)
                     /gene="rpoH"
                     /locus_tag="WIW87_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ5174888.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLAEKAEAV"
     gene            complement(1375..2913)
                     /gene="lnt"
                     /locus_tag="WIW87_001766"
     CDS             complement(1375..2913)
                     /gene="lnt"
                     /locus_tag="WIW87_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ5174889.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMHQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(2982..3350)
                     /locus_tag="WIW87_001767"
     CDS             complement(2982..3350)
                     /locus_tag="WIW87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ5174890.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(3250..3708)
                     /locus_tag="WIW87_001768"
                     /pseudo
     CDS             complement(3250..3708)
                     /locus_tag="WIW87_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            3902..4138
                     /locus_tag="WIW87_001769"
                     /pseudo
     CDS             3902..4138
                     /locus_tag="WIW87_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT          909 a          965 c         1307 g         1033 t
ORIGIN      
        1 atttgtactg tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatcaat
       61 tccgccaatc tgtcggaaaa gcagctgatg cggcagtgtc tggtgcatgt ctgctttttg
      121 atttcggcaa ttgcaacggc gtggacggat aaaatcgtgt acagcacgat acacaagcct
      181 cattggggtt tctattataa aaaaacaccc gacaaaccgg cttgtcgggt gtttaattat
      241 gtctatttca aaccgcttcg gctttttcgg caagaaaacc acgcagtttc tgcatagctt
      301 tcgcttcaat ctggcggatg cgctcggcag agacgccgta ttcggcggca agctggtgca
      361 gcgtcagccc gccgtcgtct tgaagccagc ggctttccac aatacggcgg ctcctgtcat
      421 ccagttgcgc caaagcgttt tgtaaacctt ctgtttgcag ggcgtaatgc gcctgtttcg
      481 atagttgtcg gctcggttcg gaatcgtggt cggcaagcca gtcgatgggg gcgaaactgt
      541 cctcgtcgtc gctgttgtct gccatgatgg cgatgtcgtg tcccgtcatg cgctgttcca
      601 tttccagaac ttcggaaagt ttgacaccca aatcgtcggc gatgtcttgt gcctctttgg
      661 gagacagggc gttgaggttt ttacgcatac tgcgtaaatt gaaaaacagc ttgcgttgcg
      721 gtttggtggt ggcaacgcga accaaacgcc agtttctcaa aataaactcg tggatttcgg
      781 ctttaatcca gtgtacggca aatgaaaaca gacgcgcgcc tctaccgggc tcgtagcgtt
      841 tgaccgcctt catcagtccg atattgcctt cctgaatcag gtctgcctga ttcagcccgt
      901 agccgtcata gccgcgcgcg atggaaacga cgacgcgcag gtgggacagg atgagttgtt
      961 tggcggcgtt gaggtcgccc tttatccggc gttcggcgag gcgggtttct tcttcctggg
     1021 acagcatcgg gatgctgttg acggtgtgga tgtattgttc gaggctgccg ttgccgcttt
     1081 ggatggcggg taatgcgaaa gcgttattca tttggggcat ttcctttcgg ctgaaactgc
     1141 gtatcggcgg tttgctgtgt tgggatgcag tatatcactg cttggcttgt attttgtatg
     1201 tttggcagga gatatgcgct aaggtttgaa agacaggaaa attttgtaag gcaggtttga
     1261 ttgattttgt aaacctgatg gctcaattcg atttcagaat tatattacat acgtaattgt
     1321 atgtaaatag ccggtttgaa aaaagacagc ccgtccggac gggctgtgca ggtatcagtg
     1381 ttctttgttt cggaagatga aaagaatcag tgcggctagg gtcaatatgc ccatcaacca
     1441 ccatgaactg ccggttttca tatagggcgt ttcgccgaca tagcctttga tgtgtccttc
     1501 caatacggtt tccgtatcgg gttgggcttg ggcgatgatg ttgcctttgg gggagatgat
     1561 ggcggttgcg ccggtgttgg tggcacggac catatagcgt ccgagttcca tagcccgcgc
     1621 ctgcgattgt tggaggtgct ggtgcatggc gttggatttt ccgtaccacg ccatattgct
     1681 ggcattggca agcagtgtgg catctttggc ggcggcaatc agttcgtcgc cgaatccgtc
     1741 ttcgtaacag atgttgaagg cgattttttg gtttttcatc agcagggcgg attgcttacc
     1801 gccgcctttg cggaagtcgg aaaggggcat attcatcatt ttgtaaagcg gcgtggtcag
     1861 gaagggcagc ggtttgtatt cgccgaaggg gacgaggtgg tttttggcgt agtaggggat
     1921 gccgtcctga ttgttttcct gataaccggt caggttgatg acggcgtttt cgtaaccgtt
     1981 gccgtccgaa gtgtattggc tgatgccgac ggcgagcgcg ctgccgttgt tttgcgcctg
     2041 ttcggcaaat tttgccagta tgttttctgg caggttttgg cgcataacgg ggatggcggt
     2101 ttcgggcagg atgacgatgt cggcagtggt tttgccgacc tgttcgtaat atttctgtat
     2161 ggtcgggatg acttggtctt cacgccattt gagggtttgg tcgatgttgc cttgaagcag
     2221 ggcgacggtg ctgcggctgc cgtcggggcg ggtgaagtcg gtttgtcggg cggtgtagcc
     2281 tgcggcaagc agggcggcaa tcaggataat tggaagcagg cgtttgcccg aacgtgtggt
     2341 gttatcactc gccaaaacca gccagacacc gagaaaggcg gttgccagtg taaccatgtg
     2401 gatgccgccc aatggggcaa agccggcgag cgggctgtcc ggggtgattt gggagtagcc
     2461 gattgcgccc cagccgaatc cggtcaggaa acgttcgcgg gcaaactcgg tcagcgtcca
     2521 caggatgggc agtaccaaac cgatttttat gccccgaggc agggtaaatt ttttccacag
     2581 ccagaaacac agtgccggat aaagggcaag gtaggcgggg agtaggaagg tcagcggtac
     2641 ggcatagagg tcgggcaggc cggaaacgtc gtgcagggcg gtgtgtatcc agtagaactg
     2701 tgtcgtgtat gcggtcaggc cgaacaggta ggcggaagag acagcaaaac gcggacgcag
     2761 ttcgatgagg cggacgaagg caccgaaaat caagggcatc agccaaaagt ggtagtaggg
     2821 tgcgaaggta aagggggtgg cggcggcaaa aaggatgagc aaaggccagt agagggcggg
     2881 gtgctgccag tatttgtcca gtttggaaac catattcatc tgtctgttcg gaagataccg
     2941 tctgaacatc tttcaaacgg catcggtatt tgaaaaagaa atcaatgcct gccgaaacga
     3001 ttcatcagcg gcaaggcggg ggcgcaggca atcgaaccgg aaagcagccc gacaacgagg
     3061 ttggcgaagt actccgccca gccagcgtcc cagtgttgcg cgtgcaggaa atcgtgcaga
     3121 aggccgaggt tgtgggcgac cagtacgccg ccgataagaa acatggcaag cgtgccgacc
     3181 acgctcaaac cgcgcataaa gcaaggcata aaggcagtca gcatttgccc caaactgcgc
     3241 gaaaaggttt gtgggcggcg catcagcagc atgcctaagt cgtcgagttt gacgatgacg
     3301 gcaacgattc cgtacaccaa aacagtcatg ccgatgccga ttgccgccat tacgagcatg
     3361 cgcgtcatgt cggtgcagaa acttgtgcag cagcttttct acgccgtcaa agcacagata
     3421 aatgccgcct gccgtcaaaa gcggcgtaat gagttgcggc aggaaggcgg aaagcagcag
     3481 agccgcaggt accaaaaccg gcttgttgga aaaagaacct ttcgccatcg accaaacaat
     3541 cggcaactcg cgttctgccg atacgcccgt aacccggttg gcattgggtg ccaaatcgtc
     3601 gccgaccatg ccggcggttt tctttgcggc ggctttggtc atcagggcaa catcgtccaa
     3661 aacggcggtg atgtcgtccg gcagggtaaa tagtgaggca aatgccatta aagaatcctg
     3721 aaatgcggcg caaagtccga cattatatag gagaacgcgg atttgggcgg tttcaggcgg
     3781 catgaaacag gaaaatgccg tctgaaggcg gtggcggacg tgaagtaaag tttcgtgaaa
     3841 aaaatgccgt gttacagtct ttcgatttta atttaatgaa ttttaaggga gaatcgttag
     3901 cgtggattgg atgggcagtc tgttcctgcc gggtggcgca ctgttgttcc tgagcgtggt
     3961 ttcgaccact ttgtccgcac gtttgggaat gcctttgctg ctggtttctc ccgccggcgt
     4021 gttggatggg gcggcggaag ccttggcgat tgcggcgttc ctgatgttgg tcgcgcgtcc
     4081 gccggcagtg ttcggcggtt tgtggaaatt caattatagc ctgcgtgaaa aggcgtatag
     4141 ccgaatagaa atgcagtccg acaccgtgct tcaggcgggg atttggcgtg gtacatcctg
     4201 cccgacggca aggt
//
LOCUS       DAWISC010000162         4203 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWISC010000162 DAWISC010000000
VERSION     DAWISC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.162"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..143)
                     /locus_tag="WIW87_001770"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="WIW87_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(271..1521)
                     /locus_tag="WIW87_001771"
     CDS             complement(271..1521)
                     /locus_tag="WIW87_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ5174891.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1588..1782)
                     /locus_tag="WIW87_001772"
     CDS             complement(1588..1782)
                     /locus_tag="WIW87_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174892.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGNETLKTS
                     MLNKLPDNAKENLRIMGYLP"
     gene            complement(2003..3778)
                     /gene="ggt"
                     /locus_tag="WIW87_001773"
     CDS             complement(2003..3778)
                     /gene="ggt"
                     /locus_tag="WIW87_001773"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ5174893.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQNAISAPNLLNRFGSYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3946..4182)
                     /locus_tag="WIW87_001774"
     CDS             complement(3946..4182)
                     /locus_tag="WIW87_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ5174894.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIASRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1050 a          987 c          998 g         1168 t
ORIGIN      
        1 atctctgagg taacgggttt ttgacggttc aggtatggtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaattat ggcttgtgcg
      121 gtttgccgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg cgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcagcg gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtaattttc atcggttgca ggtcaacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttaa ccaactcttc cgccatggcg gctgcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa tgcttcttta aacgccacgg ctttggcggc
      721 gataacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcaacgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga ggaccgcgca gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaacgg caccgggttg ggatattcgc cgccggcaac
      901 cagaccggca tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgtaacgc gtaggcagac gcacccgcca caatcatttt
     1021 gggtttgtgt tcgagcgcga ggcgttcgac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcgt tgtagagttt gcctgagata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcta gagacatacc caaaatggtg tcgcctggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tggacgttgg cataggctgc
     1261 gccaaacagt tcttttacgc ggtcaatcgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcgtcc attacggcgc agctgacgta gttttcggaa gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcgt attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatatt tcaaaaccta aggcagatag cccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatt
     1681 tagtacaaga ctaatcaaaa gcaatacatt aaatggtaaa ttttcggcag tttgtgcaaa
     1741 atcaatcaaa taaccagcac caaccacatc tttatcagcc ataacccgct gatacaccac
     1801 ttgctaagac aactctcctg ccccaccatg tcttaaaatc agatgaatgg cttgtaatgc
     1861 tgtgtttggt gtctcacaaa ttgccaagat ctgctcagaa gcttgcttga tggcttgtat
     1921 ttttgtttga ttactcacaa tttcccccta ttttaataat taacttaaat gcggtcaaat
     1981 tcacaaaata caagctttac ctctaatcac ccatcactcg accttctcgg cgtggatcgg
     2041 caccaccaac cagcctgctt ggctcgataa taatggcttg aacacctgaa tttagctcac
     2101 gcacatcagt cttatagccc aaatcattta atgcttgttg ccactggacg gcggttgtac
     2161 ccgtttctag ttcatagcta ccaaagcgat ttaataaatt gggtgcactg atggcatttt
     2221 ggatatccat attccagtca ctatgtgcca caatcgtctt agcgacatag ccaatgatac
     2281 ggctaccacc tggggagccg attgccatat aaggcttgcc tgctttaaat acgatggttg
     2341 gtgccattga ggagcgtggt ctcttgccgg gctcgacacg attggcgacc tgtttgccct
     2401 gctttattgg ctcaaaacta aagtctgtca gctcattatt cagcaggtag ccatttgcca
     2461 tcaaagttga gccaaacgca ttttcaatgg aagtcgtcat tgatagcaca ttgcccgcct
     2521 tatccacaat tgatatatga ctggtagaag gtaactcaat cgcttgtgag gacacccact
     2581 catgaataaa atcgcctgca gatacgctag gcaatgcctt atccgactgc tcaagcagct
     2641 ggctgcgatg ttttaggtag tctttagaaa tcaactggcg aatgggtact ggtacaaaat
     2701 cagggtcgcc caaatataca tcacgatccg caaacgcaag cctagaagcg tcgcccaaga
     2761 gacgtaaacc ttcagcatca taccccacct gattgggtga aaattcattt aaaatcccca
     2821 aaatctgacc cacagcaatc ccacctgagc ttggtgcacc cataccgcat acttcataaa
     2881 tacgataagt cacacaaaca ggcgggcgtt ccaccacttg ataatcagat aaatcttgta
     2941 aggataattg accggggtta tccttagcat tttggacaac tgaaacgata ttttgggcat
     3001 atttaccagt atgcagagct tttgcacctt gagctgctaa cgcctgaaca ctgtcagcaa
     3061 attctaaatt tttcagcaag ctgcctgctt gtagcggcac accattcggc aaaaaataag
     3121 cggctgtttt tggatagcgt gccaaatgct gctgattttg ctcaaccgag atggcaagcc
     3181 ttggcgacac ctcaaagcct tgttttgcca agcggatcgg tgtatcaaat aattttcccc
     3241 aaggcaatac accgtatcgc tgatgtattg tctccatcag tttagggata gcaggcgtac
     3301 ccaccgagcg accaccgacc accgcttcca taaatttcaa tggttgacca tctttatcca
     3361 aaaataattc cggcgtcgca cgcatcggtg ccgtctcacg cccatcaaat gtggtcaatg
     3421 ttttggcggt attatcccaa tacaacacaa atgcaccacc gcccaagcct gacgactgtg
     3481 gctctaccaa gcttagtgtc gtctgcaccg ccaccatcgc atctgcagcg ctaccgcctt
     3541 gctttaagat atcatagcca gcttgtgttg ctaatggatt ggctgacgct accataaaat
     3601 cacttgcaat cacctgcttt tgttcggtca gtcccgttgc atgttcaggc gtgtgagcgt
     3661 ctgcacctgt gatgacagca gaatgagtat taaccttacc ttgattggca tggatgactt
     3721 gacatccaga gattgtcata gacattatca atgcagtcaa taaatatgtt ttagccacaa
     3781 gcactccttc gcctgagttt gattgatgat tcatacaagg catgctgatt attgtattta
     3841 atatggctaa ataattcaat ccaaactatc aatcttgacc atcaaaaaaa gaccgctaat
     3901 gtcatcagca gtcttttttg atatttattt taagatatta agtaatcaga cctttgggct
     3961 atgctcttca atgagtggtt ttagctcacc tgattggtac atttgtagga taatatcact
     4021 accaccgatt aactcaccat taacccaaag ctgtggaaag gttggccgac tggcgatgag
     4081 tggtagagta ctgcgaattt ctgggttttc taggatatta acaaaagcaa agggtctgcc
     4141 aattgggtca gcacctctac tgcacgcgct gaaaatccac attggggaaa ctggggcgtg
     4201 cct
//
LOCUS       DAWISC010000163         4127 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWISC010000163 DAWISC010000000
VERSION     DAWISC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.163"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            43..675
                     /locus_tag="WIW87_001775"
     CDS             43..675
                     /locus_tag="WIW87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ5174895.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WIW87_001776"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WIW87_001776"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ5174896.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="WIW87_001777"
     CDS             2197..4065
                     /locus_tag="WIW87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ5174897.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
BASE COUNT          945 a         1099 c         1064 g         1019 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagctg
     1081 ccagcgagtt catccacttt gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa actggccgaa atcatcgaaa
     1201 aactgaccgg catggcgcat gagttggctg ccgtcccgat gatgagccgc acccacggcc
     1261 agcctgctac accaagcaca ctgggtaaag aaatcgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaatggc gcagtcggca
     1381 actacaatgc ccatatggtc gcttatcccg atgtggattg ggaaacccat tgccgcaact
     1441 tcgttgaaat cagcctcggc ctgaccttca acccctacac cattcaaatc gagccgcacg
     1501 actatatggc ggaattcttc caagccatca gccgtgtcaa cacgattctg atcgacttca
     1561 accgcgatgt ttggggctat atttctttgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaacccgat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gcttcttatc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccatctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gacgctactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgtcg ttacggcgta gccaatcctt atgagaagct gaaagacctg acgcgcggca
     1981 aagacggcat tacgcctgaa gtgttgaaac tctttatcga gtcgttggaa attccggcag
     2041 aagccaaagc caaattgctt gagctgaccc ccgcactgta tgtgggtaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagctt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgcct tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcagaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggt tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcctg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt cccagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tatacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga attccacaaa
     4021 cacgaacgcc tgcttaaatc ttcccacggc ggcgcattgc agtagcctgc cttcttgtcg
     4081 gatattgcct ttggcagcct atgccgctgt ttgccgttcg gggcggc
//
LOCUS       DAWISC010000164         4102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWISC010000164 DAWISC010000000
VERSION     DAWISC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.164"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..332)
                     /locus_tag="WIW87_001778"
     CDS             complement(<1..332)
                     /locus_tag="WIW87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ5174898.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WIW87_001779"
     CDS             complement(313..594)
                     /locus_tag="WIW87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ5174899.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WIW87_001780"
     CDS             complement(603..1397)
                     /locus_tag="WIW87_001780"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ5174900.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WIW87_001781"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WIW87_001781"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ5174901.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            complement(3250..4029)
                     /locus_tag="WIW87_001782"
     CDS             complement(3250..4029)
                     /locus_tag="WIW87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ5174902.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWAV"
BASE COUNT         1062 a         1197 c          946 g          897 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcgtccac gctgttttga
      121 ggaggcggcg tttccgcatc gggacgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcacgcct gtcgtccaca ccgaacagac ggagctccgc
      421 accggaaccc agtgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcccc
      481 gttgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaaccg tcagaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatatgc ggatgagggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggac aaactcgccc gcctccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcaatatta aacggcacac caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 cagggcggca aggccatttc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg gattcttctt gatttgttcc agcacattgg cgatttggtc gatatgcctg
     1081 ccgtcgggcg cgggccagtt acgccactgg tagccgtaaa ccgggcctaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct ttaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtacccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacaggca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaatatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 gtttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtgatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgat gttccggccg gcgacatcgg
     2281 cgtaggcggg cgcgaaatcg gctacctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggctc cggcaatgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggtgcga aagtactgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaacaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtggcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 cttatccgcc cgttcaaatc gggtgagact accgatacat ctgaatatgc tatgccgtct
     3241 gaacggcatt cacaccgccc aatcctgcac gcgcttcaaa tcattttgcg ccaaagtatc
     3301 tgcgtggcgg ttacggctct gatattccct gtctttcaag atgctgctcg ccacataatt
     3361 caaatgtgcc tttgccgcct ccgaagcctc gcccggccgg cggtttgata ttgcctcata
     3421 caatacacgg tgctgcgcca tcagcttcgg acgcggatct tcttcctgat tcagataaat
     3481 aaggctgctg cgcgtctgcc ggtacagcat tttcaacaaa ccgcccgaca aatggctgaa
     3541 caacaaattg tgcgccgcat cggcaatcgt ctgatgaaag ctgacatcag cttcgctctg
     3601 atgttccaaa ttgccgcttt cgcacgcctc ctcaaacttt tcaagccaaa acccaatccg
     3661 cttcaaatcg gcatccgtgc ggcgttctgc cgccaatgcc gccatacagc cctcgatgtg
     3721 gcaactgaaa tcaaaaacat cctgttccca attggaatgc ttgcccaaaa gctcctgcca
     3781 actttgcaaa aaatcctgct gcggcttgac cgaaacataa taaccgtctc cctgcctcgc
     3841 ttccaaaacc tgacgggcga ccaaaacatt caatgccgac ctgaccgacg ggcgcgaaac
     3901 gccgaactct tccgccaaaa cgcgttcggg cggaatcttg cccccttccg cgtaaacccc
     3961 ttccgcaatg cgctcctcca ataccgacaa tacctgatcg ctgattttct gaggccttac
     4021 cagtttcatc actcctcctt tataaagatt ccctgcagaa cccttccgaa atatagtgga
     4081 ttaacaaaaa tcaggacaag gt
//
LOCUS       DAWISC010000165         4054 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWISC010000165 DAWISC010000000
VERSION     DAWISC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.165"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..316)
                     /locus_tag="WIW87_001783"
     CDS             complement(122..316)
                     /locus_tag="WIW87_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ5174903.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(306..2387)
                     /locus_tag="WIW87_001784"
     CDS             complement(306..2387)
                     /locus_tag="WIW87_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ5174904.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2864..3244
                     /locus_tag="WIW87_001785"
     CDS             2864..3244
                     /locus_tag="WIW87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ5174905.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3326..3513)
                     /locus_tag="WIW87_001786"
                     /pseudo
     CDS             complement(3326..3513)
                     /locus_tag="WIW87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3526..4020)
                     /locus_tag="WIW87_001787"
     CDS             complement(3526..4020)
                     /locus_tag="WIW87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ5174906.1"
                     /translation="MIFKQNQNYWAVFDANKETLIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTRR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLEAWNAIDADDVILSLDNSPEEIGAGLKLAL
                     SHCR"
BASE COUNT          940 a         1020 c         1138 g          956 t
ORIGIN      
        1 tcggtttcgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
       61 ccactatatt ataaaaattc cgtctgaagc ggcgttcaga cggaatttgt ttcaagcagg
      121 cttaacagca gcgtccgccg tttgcgccgc agcggcgttt gcggcaatag tcttgaaact
      181 gcagcttggt catcacgggg gcgttgggat tatgtttgcg ctgctgtgca acgtagtttt
      241 cataatcggg cacgcctgcc atcaagtttg ccgtcagctt gatggttttc caccaagacg
      301 cgagcttatg cttcactttg tgcctccggc tgtttgccgt cgcggtacac cgccgggatt
      361 tctttggcgg tcggccagcc gactttgcgt gctttgaggg cggtacgcaa accgtacgcg
      421 gcgacaatca cgacaaccga caagaagagg atggtcagac cggcattaat cttgtcgttg
      481 aagatgattt gcgccatttc gccgatgtct ttggcaggcg caaggatttc gtttttagcc
      541 aatgcgtcgc tgtatttgcc ggcgtgggca aggaagctga tgcgcgggtc gctgtggaac
      601 agtttttgca ggccggcgta gcaggttacg aacagtacgc cgacggcggg aacgagtacc
      661 acccagacat aacggtcgcg tttcatctta atcagcacca cggcgcacat aatcaaggct
      721 acgcctgcca gcatttggtt ggcgatgccg aacaaaggcc agagcgagtt gatgccgccc
      781 aacgggtcgg tcacgcccgt gtagaggaag tagccccaca atgccacggc gaagaaggtc
      841 gcaatcaggt tggcggggat ggagtcggtg ttgccgaaag gtttgtagaa gatgctgccc
      901 aagtcttgaa tcataaaacg tgcgacgcgc gtaccggcat cgacggcggt caggatgaac
      961 aaggcttcaa acaacagggc gaagtgatac cagaacgcca tcatcgcctc gcccggaatc
     1021 aggcggctca taatgtgcgc cataccgact gcgagggtgg gcgcaccgcc ggcacgggaa
     1081 aggatggtgt tttcgccgac ttctttagca gtgtgcaaca gggttgcggc atcgacaggg
     1141 aattgcagct tggtggtaat cacttcggcg gcggtattgg catccgtacc gatcagggcg
     1201 gctgggctgt tcatggcgaa gtacacgccg ggatcaagcg atgcggcagc ggcaagtgcc
     1261 ataatggcta cgaaactttc catcaacata ccgccgtaac cgatcatgcg gacgtgggtt
     1321 tcgttttcca gcattttcgg cgtagtgccg gaagaaatca gcgcgtggaa gcccgaaacc
     1381 gcaccgcagg cgatggtaat gaacaagaat gggaacaatg cgcctgagaa taccggaccc
     1441 gaaccgtcga taaagtgggt tacggcaggc atttgcaaag cgggattgac gatgacgata
     1501 cccaaagcca aggccgcaat cgtaccgatt ttcaggaagg tggagagata gtcgcgcgga
     1561 gtgagcagca accatacggg caatacggag gcgacaaagc cgtaaatcat aatcgcccaa
     1621 gtgagctgga tgccgtcaag gtcgaaccaa tgcccgatgg aacttttagc cacatcttcg
     1681 ccgtaaatta ccgccagcat cagcaaaata aagccgacga tggaaatctc gccgattttg
     1741 cccggacgga tataacgcgt gtaaataccc ataaacagcg caatcggcat agttgctgca
     1801 atggtgaacg taccccaagg gctgtgaacc aatgctttta cgacaatcaa cgccaacacc
     1861 gccataatga tgaccataat catcaaaata ccgatggagg caatcacgcc ggggacagtg
     1921 ccgagttcct gtttcacaat atcgcccaaa gacttaccgt cgcggcgcat agagacgaac
     1981 aagaccatca tatcctgtac cgcgccggca aataccacgc cgaagataat ccacaaagta
     2041 ccgggcagat aacccatttg cgccgccaaa accggaccaa ccaaagggcc cgcgccggca
     2101 attgcggcaa agtggtgtcc gaacaatacg cctttgtgcg tcggaacgta gtccaagccg
     2161 tcgttgtggc gttctgccgg agtcaggcgg tcaggatcga gccgcattac gcggttggcg
     2221 atgtagaggc tgtaaaaacg gtaggcgatg cagtaaacgg atatggcggc ggtgaccatc
     2281 cataccgcgc tgacctgttc gcctcggctg agggccagag tggtaaagga tgctaagccg
     2341 accagtacca ctatgcccca aatgaggaag gttttgagtg atttcatcga ataaatcctt
     2401 atctcacact gtcggaatat gcctgaacgg cgggaaacgg ccgaaccatc tgccggcacg
     2461 ggcacaaacc ggagtctgaa tgattgacga agtatgaaac agtgcgcttg tcgggatttg
     2521 tgccttatgc ccgcatcaaa cagtgcaggg atgcgggcgc acaaaacgtt aaacgccgaa
     2581 taggatttta acgcaaatta gcacaccgat agcggttttt acttggaatt tggaaaaaat
     2641 ttacattcct ccgggcgggc aggcaggttc agacggcatc gtcaggcaaa aggcggcatc
     2701 ggaagagggg taaagaaggg gcgcgcaatc cggattattg attcatcgca ggtaaattcc
     2761 ggttatcggg cttgtgtgtt tgcgcgtccg tttatagtat ggcgttgccg cagcttggaa
     2821 tcagggcggt tgtttcatat cttattttta ttggggagct tttatgaata tcaggttttt
     2881 cgcgctgacc gtaccggttt tgtctttggc ggcctgtgcc gtgccggagg cgtatgatga
     2941 cggcggacgc gggcatatgc cgcccgttca aaaccaagcc ggcacggacg attttcgggc
     3001 gttttcctgc gagaacggtt tgtctgtgcg cgtccgccat ttggacagcg gcaaagtcgc
     3061 gttgcggctg gacggcaggc gtgccgtcct ctcttccgac gttgccgcat ccggcgaacg
     3121 ctataccgcc gaacacggtt tgttcggaaa cgcaaccgag tggcaccaga aaggcggcga
     3181 agcctttttc ggctttaccg atgcctacgg caattcggtc gaaacttcct gccgcgcccg
     3241 ttaaacgggg cagcctgttt gaaccgccgt ccagattttt cgggcggttt tttgtgtcgg
     3301 acggacggtt ttttgtgtcg gatttgtttt gcagttcggc ctccggcagg gttcgggctg
     3361 cctgataccg tccgtaactt cgggtattgc cgagttcgtc ggcaacggcc gctgcggctt
     3421 gggcggcgtt ttgccgaacg cctgcgttac ttccctttgt aggtcgatct ctttggcaac
     3481 cgcgtctttg tcaaagctgt ttttcagacg gccttttgtc aaatattatc ggcagtggct
     3541 caatgccaac tttaaacctg ctccgatttc ttcagggctg ttatccaatg ataaaatgac
     3601 atcgtctgca tcaatggcat tccacgcttc cagcttgaca tggcggctcg ggctgatttt
     3661 caggcagccg ttgtgcagcc aaatatccac gctcatcatg tttttaaata gggcgcgtct
     3721 ggttttatag cccaagttcc cgcatagctt ggcaacccaa tcctcatagc gttgccgaat
     3781 tttttcggta tcaaaaaaat cttggtcttc tggactgtca taaacgaaag tcctgctgtt
     3841 tgccaacgct tgcaagactg tcgtgcctaa agtttcattg tcggtatcca atggcaggat
     3901 atggggggga tataggtggt ctattgccgt taaccccaaa cctgaacatg tttgaacaat
     3961 cagagtttct ttattagcat caaaaactgc ccaataattt tgattctgtt taaaaatcat
     4021 tatttgatct ccgtaatttt gactgtatag tgga
//
LOCUS       DAWISC010000166         3938 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWISC010000166 DAWISC010000000
VERSION     DAWISC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.166"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(473..1513)
                     /gene="queA"
                     /locus_tag="WIW87_001788"
     CDS             complement(473..1513)
                     /gene="queA"
                     /locus_tag="WIW87_001788"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ5174907.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATARLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRHAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            1630..1887
                     /locus_tag="WIW87_001789"
     CDS             1630..1887
                     /locus_tag="WIW87_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ5174908.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            1869..2324
                     /locus_tag="WIW87_001790"
     CDS             1869..2324
                     /locus_tag="WIW87_001790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ5174909.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2436..3797
                     /gene="accC"
                     /locus_tag="WIW87_001791"
     CDS             2436..3797
                     /gene="accC"
                     /locus_tag="WIW87_001791"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ5174910.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGLIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          949 a         1193 c          960 g          836 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa atggcgtatg
      181 taatatgttt atacgaccaa aacggaatga attttaacgt attgcgcgtc atcaacaatg
      241 actgagtttc tcgcctctcg cgcctgaatc tatagtggat taacaaaaac cagtacagcg
      301 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgttgccttg tcctgattta
      361 aatttaatcc actatatgtc atgcagttcc tttcattcaa atcaacaaaa gaatgccgtc
      421 cgaacgtccg ttcagacggc acttgtcttc ccacaataga cttgaggctg ttctaacgta
      481 ccaccccttc gttccgcccc aaaaccatcg catcgccgta gctgaagaaa cggtattcgc
      541 gttcgaccgc atgacgatac gcggcgcgga tatgacccat acccgaaaac gcgctgacca
      601 acatcagcag cgtcgatttc ggcaaatgaa aattggtaac cagtctgtcg acaacattaa
      661 aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg ccccgctttc agacgagccg
      721 tcgcacgcgc ggcagattcg agggcgcgca tggaagtcgt gccgaccgcc cagactttgt
      781 tcccccgggc ttttgccgcc tcaacggcgg cggcggtttc agacggcact tcaaaccatt
      841 cgctgtgcat tttgtgctct tcgattttgt ccacacgcac gggttggaac gttccggcac
      901 cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg tgcaaaagtt
      961 cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat ttggcataaa
     1021 cggtttgata acggctgtcg tcatccgcat cggcggcgcg ttcgatataa ggcggcaggg
     1081 gcaggtgtcc gttctgttcc aaaagttcgt aaacggtctc tccgccttca aaacgcaggc
     1141 agaacagttc gccctcacgc ccgaccgtca cggcgcggat gccgccttca aacaccagcc
     1201 ccataccggg cttgggcgat ttggacgaac ggatgtgcgc cagtgcggta tggttgtcca
     1261 acacgcgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca aacagccgcg
     1321 ccttcatgac tttggtgttg ttgaacacca aaacgtcgcc cgcctcgaca taatccggca
     1381 aatcgccgaa cacccggtct tgcagcggca tatcgggcaa cgcaaccaaa aggcggctgc
     1441 tgccgcgcac ttcgggcgga tgctgggcaa tcagcttttc gggcagggta aaatcaaaat
     1501 ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc actttagccc
     1561 tttttcagac ggcatctttg tccgaaaaac caacagatta gaataaacac tcttaacctg
     1621 gaacatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc gccgtttttt
     1681 gatattttac tggacattta ccgacaactt cgggaaaata aacacattct cacggtcgtt
     1741 ttccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc gttgccgcaa
     1801 gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca gcctttaaag
     1861 gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa gaatcgggta
     1921 tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg catcacccga accatcgccg
     1981 ccgcacccgt ttacgccgcg cccgtacctg ccgccgcgcc ggccgtaacg cctgccgccg
     2041 cacccgttgc ggcatccgcg cccgccgccg cacctgccgc ccgcgatttg tccgacgcgc
     2101 aaaaatcgcc tatggtcggc acgttctacc gcgcacccgg cccgaatgcc gcgccttttg
     2161 tcgaagtcgg ccaacaagtt aaagccggcg acacgctgtg catcatcgaa gcgatgaagc
     2221 tgatgaacga aatcgaagcc gaaaaatccg gcacggtcaa agaaattttg gtcgaaaacg
     2281 gtacgcccgt cgaattcggc gaaccgctct tcattatcgg ataatcctgt tttcagacgg
     2341 cataaacttc cgatgccgtc tgaaatgctt tcccccttca gcgttcccgc accctttttt
     2401 acggacgggt tgccggaacc gcaggaaagg tcatcatgct gaaaaaagtt ttaatcgcca
     2461 accgaggcga aatcgcatta cgcgtactcc gtgcctgccg cgaaatgggc attgccaccg
     2521 tcgccgtgca ttccgaggcc gacaaagaca gcctgcacgt caaactcgcc gacgaatccg
     2581 tgtgcatcgg ccctgccgct tccgcgcaaa gctaccttaa cgtccccgcc attatcgccg
     2641 ccgccgaagt aagctgcgcg gacgctgtcc atccgggtta cggtttcctt gccgaaaacg
     2701 ccgatttcgc cgaacaggtc gagcagtccg gctttacctt tatcggcccg aaacccgaca
     2761 ccatccgcct gatgggcgac aaagtctccg ccaaacacgc gatgatagcg gcaggcgtac
     2821 cctgcgtccc cggttctgac ggcgcattgc ccgacgacgg cgaagaaatc ctcaaaatcg
     2881 ccgataaagt cggttatccc gtcattatca aagcctctgg cggcggcggc ggccgcggta
     2941 tgcgcgtggt cgagaaaaaa gaagacctcc tccaatctgt cgaaatgacc aaagccgaag
     3001 caggcgcggc attcggcaac ccgatggttt acatggaacg ctatttgcaa cgtccgcgcc
     3061 acgtcgaaat ccaagtgatt gccgacgaac acggcaacgc catctacctt gccgagcgcg
     3121 actgttcgct gcaacgccgc caccaaaaag tcatcgagga agcaccggct ccgttcatca
     3181 ctgaaaaaga acgcgccaaa atcggcaacg cctgtgccga tgcctgcaaa cgcatcggct
     3241 accggggcgc gggtacgttt gagtttttat acgaagacgg cgaatttttc tttatcgaga
     3301 tgaacacgcg cgttcaggtc gagcatccgg ttaccgagct catcaccggc gtggacatcg
     3361 tgcaggagca actccgcatc gccgccggcc tgcctttgca atacaaacaa aaggatattc
     3421 aagtcgaagg ccacgcgttt gagtgccgta tcaacgccga agacccgtac aacttcattc
     3481 caagcccggg cctgattgaa agctgccacc tgcccggcgg cttcggtatc cgcgtggaca
     3541 gccacattta ccaaggctac cgcatcccac cgtactacga cagcctgatc ggcaaaatct
     3601 gcgtacacgg caaaacgcgt gaacaggcaa tggcgaaaat gcgcgtcgca ctcgccgagc
     3661 tggcggtaac cggcatcaaa accaatacgc cgcttcaccg cgacctgttc gccgatgcgg
     3721 gtttccaaaa aggcggcgtc agcatccact atttggaaca ctggctggaa gatcgcaaag
     3781 ccaaacagga caagtaaacc gccgccgata tgccgtctga agccgcccgt ccgcgttcag
     3841 acggcatttc ccttgccccg cgccgtctga aaccgatttc gatatagtgg attaacttta
     3901 aaccagtacg gcgttgcctc gccttagctc aaagagaa
//
LOCUS       DAWISC010000167         3917 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWISC010000167 DAWISC010000000
VERSION     DAWISC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.167"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(205..1056)
                     /locus_tag="WIW87_001792"
     CDS             complement(205..1056)
                     /locus_tag="WIW87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ5174911.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1404..2918
                     /gene="katA"
                     /locus_tag="WIW87_001793"
     CDS             1404..2918
                     /gene="katA"
                     /locus_tag="WIW87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ5174912.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2994..3194
                     /locus_tag="WIW87_001794"
     CDS             2994..3194
                     /locus_tag="WIW87_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174913.1"
                     /translation="MPSETLSDRFRRHSPIPPRRFSGRFLLNAKYPVIPTKVGIKDSG
                     LEKPFYPISFRTDNSGFPPARE"
     gene            3314..3880
                     /locus_tag="WIW87_001795"
     CDS             3314..3880
                     /locus_tag="WIW87_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ5174914.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          896 a         1019 c         1081 g          921 t
ORIGIN      
        1 aaacccccgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg
       61 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      121 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct tttggtttgt
      181 tgtctttata agattattcg gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      241 ctcaaaggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc cgcggaaagc
      301 cagcaggttg gcgagcgcct cgtcagaata cggcgtgggt gtggacgagg caaaaccagc
      361 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      421 ggtttggggc aggcgttttt ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct
      481 gtcgagggcg tttcggacga tatgcagggc ggagggtttg gcagcacatt cgcccgatct
      541 ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa
      601 ttttttcaat gcggtcaggg cttgtttcag cattgcttca tccaacatcc actccaaggg
      661 cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc
      721 gatgaggatg tggacacagg cggattcttg gtcggaaagc gggtgttcgc atacttgcgc
      781 gtgcaggtat tgcttgaaat cctgctcgcc ggcgatgttg gacagcatat cttcgccttc
      841 gtctccgccg atttgacggg caggcgcagt gtaatggctg aactcggcat cggaaaattc
      901 atccgtgtct ttgcgttcga gcagggggtt gtccgacagc cagttttcga cctcgcgttc
      961 aagttcgata cccgacatct gcaatacgcg caaagattgt tgcagccgct ggttgagctg
     1021 ctgggtctgt ttgagcttta ttccgagtaa ggtcatgata atgtggggaa aattgttatt
     1081 ttcagcctgt cggcgcaaaa aatgccgcaa agcgtcattg cattataaat ggttttaatg
     1141 agcgggttcg gattccgttc gataacaaaa aacaaacgaa aatcaagaac cgattgctta
     1201 taataatatt aaatcgattt catagtttta atagcaaaaa tcttggcgta tagtcgcatc
     1261 catagttttt acaaaaggga aataaaatgt cgattcaaga aatttattgc aatcaagaaa
     1321 ccggttacga atacgctttc cgccaaatcg tactgtaagc cgtttacccc catttgacca
     1381 acctgagaaa aggaacaaga gcgatgacta cctccaaatg ccctgtaacc catctgacca
     1441 tgaacaacgg cgcgcctgtt gccgacaatc aaaacagcct gaccgccggt cctcgcggcc
     1501 ctctgctggc gcaggatttg tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca
     1561 tccccgaacg ccgtatgcac gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc
     1621 acgacatcac caaatacacc cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga
     1681 tgttcgcccg tttcaccacc gtggcaggcg aacgcggcgc agccgatgca gaacgcgaca
     1741 tccgcggttt tgccttgaaa ttttataccg aagaaggcaa ctgggatgtg gtcggcaaca
     1801 acacgcccgt gttcttcctg cgcgacccgc gtaagttccc cgacctgaac aaagccgtca
     1861 aacgcgaccc gcgcaccaat atgcgctctg ccacaaacaa ctgggacttc tggacgctgc
     1921 tgcccgaagc actgcaccaa gttaccatcg tgatgagcga ccgcggcatc cccgccggct
     1981 accgccatat gcacggcttc ggttcgcata cctacagctt ctggaacgaa gcaggcgagc
     2041 gtttttgggt gaaattccat ttccgcaccc aacaaggcat taaaaacctg accaacgaag
     2101 aagccgccaa aatcatcgcc gacgaccgcg aaagccatca gcgcgactta tacgaagcca
     2161 tcgaacgcgg cgagtttccg aaatggacga tgtacatcca agtcatgcct gaagcagacg
     2221 cggaaaaagt accttatcat ccgtttgact tgaccaaagt ttggccgaaa aaagactatc
     2281 cgctgattga agtgggcgaa ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg
     2341 tggaacaatc cgccttcgca ccgagcaacc tcgttcccgg tgtcggcgcc agcccagata
     2401 aaatgctgca agcgcgtttg ttcaattacg ccgacgcaca acgctaccgt ttgggcgtaa
     2461 acttccgcca aattcccgtc aatcgtccgc gttgccctgt tcacagcaac cagcgcgacg
     2521 ggcaaggccg cgccgacggc aactacggca gcctgccgca ctacgaaccc aacagcttcg
     2581 gccaatggca gcaacaaccc gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg
     2641 cacactggga ctaccgccaa gacgatgacg actatttcag ccaaccgcgc gccctgttca
     2701 acctgatgaa cgacgcgcag aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg
     2761 acgcgcccga cttcatcaaa taccgccata tccgcaactg ctaccgttgc gacccggcat
     2821 acggcgaagg cgtggccaaa gcccttggac tgactgtcga agatgcccaa gccgcccgcg
     2881 cgaccgatcc cgcactgggt caggctggtt tgctgtaagg gggcattatg tggatggaaa
     2941 ttgaagaaat cctgtcccgc gccgtccgct atcggaaata accgggcata aaaatgccgt
     3001 ctgaaacatt gtccgaccgt ttcagacggc attcccccat cccgccccgc cgtttcagcg
     3061 ggcgtttttt attaaacgca aaatatcccg tcattcccac gaaagtggga atcaaggact
     3121 cggggttgga gaaaccgttt tatccgataa gtttccgcac cgacaactct ggattcccgc
     3181 ctgcgcggga atgacgggat ttctgttttt gattttttgt ttttgcggga atgacgggat
     3241 gcgggttttc gtgcggcatt tttgcatttt tttgcttttg ctataatccg ccctttttga
     3301 ggacgggtgc ggtatgggtt tttatgcttt gctcttgatt gctctgggga tgtcgatgga
     3361 tgcgtttgcc gtcgcattgg caaagggtgc ggcggtcaga atgcctccgc gcaaaattgc
     3421 ggcaacggct ttggtgttcg gcacggttga agcgctcacg ccgctggcag gctgggtagg
     3481 cggtttttat gccaagccgt ttatcagcga atgggaccat tgggtggctt tcgtcctgct
     3541 gggcgggctg ggtctgaaaa tgatgcgcga agggctgtcc ggcgaggcgg aagatgtgcg
     3601 cgaaagcaaa cgggaaagcc tatggatgac ggttttgact gcttttggaa ccagtattga
     3661 ttccatgata gtcggggtgg gcttggcgtt tatggaggta aacatcgcct ttgccgccgc
     3721 aatcatcggt atggcgacga cggtgatggt ggcggtcggg ctgacggcgg gaagggcttt
     3781 gggcgtattg ttcggcaggt gtgcggaatt tgccggaggt ttggtgttga ttgccatcgg
     3841 cacatggaca ctcttgtcgc atttgggttt gattcaatga tgtcggaaaa tatagtggat
     3901 taacaaaaac cagtacg
//
LOCUS       DAWISC010000168         3911 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWISC010000168 DAWISC010000000
VERSION     DAWISC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.168"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            202..1719
                     /locus_tag="WIW87_001796"
     CDS             202..1719
                     /locus_tag="WIW87_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5174915.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTPLADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1778..3559)
                     /locus_tag="WIW87_001797"
     CDS             complement(1778..3559)
                     /locus_tag="WIW87_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ5174916.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFANCADIDAESWLARHSDGLPAGIST
                     PQPSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFIKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          818 a          878 c         1288 g          927 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaatgtgaa
       61 atccgccctt tgaaaatcgg gcggcggttt ttgtttgcct gctttcaggc ggcaaagccg
      121 gttttcacgg gtttctgcct gtttttcgga tggtttgacg tgcttcggcg gcgtgtttgc
      181 cagaaaggta aatgacaggg tatgttgtat ttcagatacg gctttttggt tgtttggtgt
      241 gcggcaggtg tttctgccgc ctatggggcg gatgcgcccg cgattttgga tgacaaggca
      301 ttgttgcagg tgcagcggtc ggtgtcggat aagtgggcgg aatcagattg gaaagttgaa
      361 aatgatgccc cgcgcgtggt tgacggggat tttttgttgg cgcatccgaa aatgttggaa
      421 catagtttgc gcgacgcgct caacggcaat caggcggatt taatcgcttc gttggcggat
      481 ttgtatgcca agctgccgga ttatgacgcg gttttgtacg gcagggcgcg ggctttgctg
      541 gcgaaattgg cgggaaggcc ggcggaggcg gtggcgcggt atcgggaact gcacggggaa
      601 aatgcggcag acgagcggat tttgctggat ttggcggcgg cggagtttga cgatttccgg
      661 ctgaagtcgg cagaaaggca ttttgcggag gcggcaaaat tggatttgcc ggcaccggtt
      721 ttggaaaatg tggggcgttt tcggaaaaaa acggaggggc tgacgggctg gcgtttttcg
      781 ggcggcatca gtccggcggt caatagaaat gccaataatg ccgcgccgca atattgccgg
      841 caaaacggag gccggcagat atgcagtgtc agccgggcgg agcgggcggc agggttgaat
      901 tatgaaatcg aggcggaaaa gctgacgccg ttggcagata atcattattt gttgttccgt
      961 tccaatatcg gcggcacgag ctattatttc agtaaaaaat cagcttatga tgacgggttc
     1021 ggcagggcgt atttgggttg gcagtataaa aatgcacggc agacggcggg gattttgccg
     1081 ttttatcagg tgcagttgtc gggcagcgac ggctttgatg cgaaaacaaa acgggtaaac
     1141 aaccgccgcc tgccgccgta tatgctggcg cacggagtcg gcgtgcagct gtcccatact
     1201 taccgcccaa acccgggatg gcaattttcg gtcgcgctgg aacattaccg ccaacgctac
     1261 cgcgaacagg atagggcgga atacaataac ggcaggcagg acgggtttta tgtttcgtcg
     1321 gcaaaacgtt tgggcgaatc ggcaactgtg ttcggcggct ggcagtttgt gcggtttgtg
     1381 ccgaaacgcg aaacggtggg cggcgcggtc aataatgccg cctaccggcg caacggtgtt
     1441 tatgccggtt gggcgcagga gtggcggcag ttgggcggtt tgaacagtcg ggtttccgcg
     1501 tcttatgccc gccgcaacta taagggcatt gcggctttct cgacagaggc gcaacgcaac
     1561 cgcgaatgga atgtctcgct ggctttgagc cacgacaagt tgtcgtacaa aggtatcgtg
     1621 ccggcgttga attatcgttt cggcaggacg gaaagtaatg tgccgtatgc gaaacgccgc
     1681 aacagcgagg tgtttgtgtc ggcggattgg cggttttgaa tggtgggata atgccgtccg
     1741 aactttggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg gcggcaaata
     1801 cgccgcgcaa ggcgttggag aggcggattt cttcggcttc ttgcagtgtt ttttgtgtga
     1861 tgtgtgtttc gattacttga tttgtttgca aatatttttg cggttcgtcc aatacggctt
     1921 ggcgcattat gccgtttaaa atgtctaaat ctaaagaggg tgtgagccat tgtccgcgat
     1981 gtttgatgaa gacgttgctt ctgccgcctt cgagcaggat gccgtctgaa ttgaaaaaca
     2041 ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag agggcgcggc
     2101 aggtggtttt gaagcggcgc aggtagtttt gtgcgggcag gacggcgggc gaaatgatga
     2161 cgcgttgttt gtcggtcaga cggtttaaaa cggcgcggga caggctgatg ccgtctgaag
     2221 cgagcagggc tttgacgcgg aacgcgccat cgggcaagtc ggcaatgtat tgtttgattt
     2281 gattttcgca gccgtcgggc aggggcaggt tgagggcttg ggcggaggtt ttcagacggc
     2341 ataggtggcg gtcgagcagg gtgcagcgtc cgttttccgc gcgcagggtt tcaaaaatgc
     2401 cgaagtcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcg cgatattcgg
     2461 cggcggggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct tgataaatgc
     2521 cgtctgaaag cggcgtgagc gacaaggtgc ggataacgac gttgaacgtg ccttcaaacc
     2581 ccaagccgcc ggaacacggg ttcaaatagc cgatgctgcc cgtataaagt ccgcgcgctt
     2641 cggcttcgag cgattcgata atctgcatac tcattttttt gggcgcgccg gtgatgctgc
     2701 cgcaggggaa ggcggcgcgg aggatgtcgg cgaacgaggt gtgcggcaag gcttgggctt
     2761 ggatggtgct ggtcatctgc caaacgctgc cgaaacgcga tactttaaac ggttcgggta
     2821 cgcatactgt gccggtttgg gcgattttgc cgagatcgtt acgcagcaaa tcgacaatca
     2881 tcacgttttc ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg cgttcgtctt
     2941 gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg gtgccgtccg
     3001 aaccgatttt gaggaagagt tcgggcgaga aacacagcgt ccacgcggat tgcccctccg
     3061 catcgggcag gtgggacaag acggcatagg ggacgggctg gcgcaggcgg cggtagaggc
     3121 tgacgggatt gccgtaggct tgcaggtgca ggcgggtggt gtagttgatt tgataggtgt
     3181 cgccgcgccg gatggcttcg tggatttggc ggatgcggtc gaggtaatcg gtttcggata
     3241 cggagggttg cggcgtggaa atgccggcgg ggaggccgtc tgagtgtcgg gcaagccagc
     3301 tttcggcatc gatgtcggcg cagttggcaa accagtgcag ggcaagattg ccgccgcgtt
     3361 cggactcaac ccccgtcagc ggcaaaccga atccgtagtc tgcaaacaac accgaatgca
     3421 gccctttttg ccagcccgat tgcagcgcgc cgtccaaagc atcgagttct tcgggacgga
     3481 aaaaacggct ttccacatga ttttgatagc gttttgcgcg gccgcttacg gcatcgtcaa
     3541 acagggcgaa ataaggcatg gcaatccggg gcaaatgttt tgattatacg ccccttatta
     3601 cacatatttt cagacaattc ggcaaatatc ggcaaaatgt aattttatgt agagaaagcg
     3661 ggggcgaagg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa tacaataagg
     3721 agacctttat ggcagaccac cagctgcaac cgtttgaaaa cgtagaatta ggcgaaaagc
     3781 aagaccagct ccaagtatag tggattaaca aaaaccagta cagcggtgcc tcgccttagc
     3841 tcgaagagaa cgattctcta aggtgctgaa gcaccgagtg aatcggtttc gtactatctg
     3901 tactgtctgc g
//
LOCUS       DAWISC010000169         3859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWISC010000169 DAWISC010000000
VERSION     DAWISC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.169"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIW87_001798"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIW87_001798"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ5174917.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRIGMA
                     AAALNCRLKRCVLPDEVFAECEQKLLHPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGKGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVFD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIW87_001799"
     CDS             1439..1924
                     /locus_tag="WIW87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ5174918.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIW87_001800"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIW87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ5174919.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          794 a          946 c         1070 g         1049 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggc
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtatc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc agatgaagtg
     1021 tttgccgaat gcgaacaaaa gctgctccat cctgaaccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgccagggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttctgg caagggtgcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt atttgatgcg
     1321 gcggaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacgg ccgttgtgtt ttttggcttt
     1501 cggtttcggc agcgggctgg ctccgttcgc gccgggcaca ttcggcactt tggcggcact
     1561 gcctttggcg tttgtgctga ttttgctcgg catagacggg ctactgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccac ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttgtcggctt cgtattcctg atagttgccg tcgaagaaca cccatttaga
     2101 gtcgccttca cacgccaaga tatgcgtggc gatgcggtcg aggaaccaac ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcaagg tcgttagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 ttttgccaag tgcagacgac cgcgttcgcc gccagacaat tgacctgcaa ttttgctttg
     2341 gtcgctgcct ttgaagttga aacgccccaa atattggcgg gcgggaattt caaactgacc
     2401 aacctgcaaa atgtcgcggc cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgtccgattt tcacctcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagc gtagatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 cgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWISC010000170         3825 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWISC010000170 DAWISC010000000
VERSION     DAWISC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.170"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            358..1479
                     /gene="dnaJ"
                     /locus_tag="WIW87_001801"
     CDS             358..1479
                     /gene="dnaJ"
                     /locus_tag="WIW87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ5174920.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1675..3693
                     /locus_tag="WIW87_001802"
     CDS             1675..3693
                     /locus_tag="WIW87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ5174921.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIVIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(3737..>3825)
                     /locus_tag="WIW87_001803"
     CDS             complement(3737..>3825)
                     /locus_tag="WIW87_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5174922.1"
                     /translation="QTAPLLSPKDLAGKTWAQAEKLRLPLYR"
BASE COUNT          810 a          861 c         1146 g         1008 t
ORIGIN      
        1 tgctggcgaa gctgaaggat caggcttccg tcggcgcgcc cgcaccaaaa ggcccagcca
       61 aaccagtgct gcccgcggat aaaaaataaa atagggggaa gtctgcagcc gcatcaaatg
      121 ccgtctgaac atgcgttcgg gcggcgtttt tataacaaaa acacttcatg gcggttggtt
      181 ttatgcctat ctaagttttt gtgtcgtgca tacctgaaga tttcagacgg catcggttta
      241 tgctgtctga aaagtgtatt ccgtttcagt ttgtaagcta tggcagtctg tttgtcttgt
      301 gttttgcgca attgccctta ttttgagccg tgattttatt ttgaattaga tgaaaaaatg
      361 agtaatcaag atttttatgc gacgctgggt gtggcaagaa cagctaccga tgatgagatt
      421 aaaaaagcct accggaaatt ggcgatgaaa taccatcccg accgcaatcc tgacaataaa
      481 gaggcggaag agaagtttaa agaagtacaa aaggcgtatg aaactttgtc cgacaaggaa
      541 aagcgcgcta tgtacgacca gtatggtcat gcggcgtttg aaggcggcgg acaggggggc
      601 ttcggagggt ttggcggatt tggcggtgcg cagggttttg actttgggga tattttcagc
      661 caaatgtttg gaggcggttc ggggcgcgcc cagcctgatt atcagggtga ggacgttcaa
      721 gtcggtatcg aaatcacgct tgaagaagcc gcaaaaggtg tgaagaaacg catcaatatt
      781 ccgacttatg aagcgtgtga tgtctgtaac ggcagtggcg cgaaaccggg gacatccccg
      841 gaaacctgcc cgacttgcaa aggttcgggt acggtgcaca tccagcaggc gattttccgt
      901 atgcagcaga cttgtccgac ctgccacggt gcgggcaaac acattaaaga accttgcgtc
      961 aaatgccgtg gcgcggggcg gaataaggcg gtcaagacgg tggaagtcaa tattcccgcc
     1021 ggtatcgatg acgggcagcg tatccgtttg agcggcgaag gcgggccggg tatgcacggt
     1081 gcgcctgccg gcgacttgta tgtaaccgtc cgcattcggg cgcataagat tttccaacgc
     1141 gacggtctgg acttgcattg cgaactgccg atcagttttg ccacggctgc tttgggcggg
     1201 gagttggaag tgccgacctt ggacggaaag gtcaagctca ccgtccccaa agaaacccaa
     1261 accggcagga ggatgcgcgt gaagggtaag ggtgtcaaat ctttacgcag cagcgcgacc
     1321 ggcgatttgt actgccatat tgttgtcgaa acgcctgtca atttgaccga ccgtcaaaaa
     1381 gagcttttgg aagaatttga gcggatttct accggcttgg aaaaccaaac accgcgcaag
     1441 aaatcgtttt tagacaagct gcgcgatttg tttgattgat tttaaggttc ggaaacaagc
     1501 agccgtatcg gggaatctcc ttgatacggc tgtttttatt tgtttaaaaa tagtttttat
     1561 tttcaatggg gtatgaggca gggtgggata actgttttta actgttcttt ttaaaacttg
     1621 acatcatggc gtgatgccaa caatatgtga acgtctgttg tcaaaggaag aataatgaat
     1681 aaatctttat ccagttcggt agaagaatac cgcgagctga cgctccgagg catgatactc
     1741 ggtgcattga tcactgtaat ttttactgcg tccaatgttt acctcggttt gaaagtcggg
     1801 ctgacctttg cctcgtcgat tccggcggcg gtgatttcga tggcggtttt aaagtttttc
     1861 aaaggcagca atattttgga aaacaacatg gtgcagaccc aagcctcggc tgcgggtacg
     1921 ctttcgacca tcatcttcgt cctgcccggt ttgctgatgg cgggctactg gagcggtttc
     1981 ccgttctggc agacgacgct tttatgtatt gccggcggga ttttgggggt gattttcacc
     2041 attcctctgc gttacgcaat ggtggtgaaa agcgatttgc cttatccgga aggtgtggcg
     2101 gctgctgaaa ttttgaaagt gggcggtcat gaagaagggg ataaccgtca gggcggcagc
     2161 ggcatcaaag agctggcggc cggcggtgcg ttggcgggat tgatgagctt ttgcgccgga
     2221 ggtctgcgcg tgattgccga cagcgcgagt tattggttta aaagcggtac ggcgattttc
     2281 cagctgccga tgggcttttc actggcattg ttgggcgcgg gctatttggt cggactgacg
     2341 ggcggtatcg ccatcctgtt gggcatttcg attgcttggg gcattgccgt gccgtatttc
     2401 tcctcacaca ttccgcaacc ttccgatatg gaaatggcgg cgtttgcgat gaagctgtgg
     2461 aaggagaaag tgcgttttat cggtgcgggg actattggca ttgcggcggt ttggacgctg
     2521 ttgatgctgc tcaagccgat ggtggaaggc atgaagatgt cgttcaagag ttttggcggc
     2581 ggtgcgcccg ctgcggaacg cgccgaacag gatttgtcgc ctaaggctat gattttttgg
     2641 gtgctggcga tgatgtttgt tttaggcgtg tcgttttacc actttatcgg cgattcgcac
     2701 attacgggcg gcatggcttg gcttttggtg gtcgtttgca cgcttttggc ttccgtcatc
     2761 ggctttttgg tcgccgccgc ctgcggttat atggcaggtt tggtcggctc gtcttccagc
     2821 ccgatttccg gcgtgggcat cgtgtccgtc gtcgttattt cactggtttt gctgctggta
     2881 ggcgaatccg gaggtttgtt ggcggatgag gctaaccgca aatttttgct ggcactgact
     2941 ttgttttgcg gctcggcagt aatctgcgtg gcttcgattt ccaatgacaa cctgcaagac
     3001 ttgaaaaccg gctacctgct caaagccacg ccttggcggc agcaagtcgc cctgattatc
     3061 ggctgtatcg ttggtgcgct ggttatttcg cccgtgttgg aactgcttta cgaagcctac
     3121 ggctttaccg gcgcaatgcc gcgcgaaggc atggacgcgg cgcaggcttt ggcagcccct
     3181 caagcgactt tgatgacgac catcgcgtcg ggcattttcg cccacaacct tgaatgggtc
     3241 tatatcttta ccggtatcgt gattggagca gtattaatcg tcgtcgattt ggtgttgaaa
     3301 aaatcatcag gcggcaaact tgccctgccc gtccttgcgg tcggtatggg tatttatctg
     3361 ccgccgtccg tcaatatgcc catcgtggca ggcgcggtgt tggcggcggt gttgaaacac
     3421 atcatcggta aaaaagcgga aaaccgcgaa ggccgtctga aaaacgccga gcgcatcgga
     3481 accttgttct ccgccggcct gattgtcggt gaaagcctga tcggtgtgat tatggcgttt
     3541 attattgcct tctccgtgac caacggcggc tcggatgcgc cgctcgcgtt gaatctgcaa
     3601 aactgggatg ccgccgcttc ttggctgggt ttggcgttct tcgttaccgg gatgtttttc
     3661 tttgcacagc gcgtactgaa ggcgggcaag taggctgtcg gaaaaaatgc cgtctgaaac
     3721 gttcagacgg cattttttat cggtaaagcg gaaggcggag cttttcggct tgcgcccacg
     3781 ttttgccggc aaggtctttg ggcgacagca gcggcgcggt ttgaa
//
LOCUS       DAWISC010000171         3760 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWISC010000171 DAWISC010000000
VERSION     DAWISC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.171"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(31..1941)
                     /locus_tag="WIW87_001804"
     CDS             complement(31..1941)
                     /locus_tag="WIW87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5174923.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
     gene            complement(1960..2604)
                     /locus_tag="WIW87_001805"
     CDS             complement(1960..2604)
                     /locus_tag="WIW87_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5174924.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WIW87_001806"
                     /gene_synonym="opcA"
     CDS             complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WIW87_001806"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ5174925.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT          918 a          856 c          948 g         1038 t
ORIGIN      
        1 gtttgaacat ttttttcttc ctgtttgatt tcagacggca ttgccgttcc gtttggtttc
       61 cagcagctcc cagcgttcca gcttttccaa aagcagcatt tcgatttctt cggcgcggtt
      121 ttgcaatgca cctgcttttt cgtaatcttt gaaaatttca ggataggaaa gttgggtatt
      181 gatttcagcc tgctcggctt ccaaagcggc gatttcgtcg ggcagggcat cgagttcgcg
      241 ctgctctttg taggacagtt tgaccgtgcg gttggctttg ggtttttctt tggcgggttc
      301 ggcatcggat gctttgggtg cggatgccgt ctgaatttta tcttcccgcg attttgcgtc
      361 gatatagtcc tgatagccgc cgatgtattc tttcagacgg ccttgtcctt cgaaaacaat
      421 gctttgggta attacgttat caaggaacat acggtcgtgc gagacaagga atactgtgcc
      481 ttgataatcg cgcaacaggt cttcgagcag ctcttgggtg tcgatgtcta agtcgttggt
      541 cggttcgtcc aagaccagga tattggcagg acgggtaaag agttttgcca gcaaaaggcg
      601 gttgcgttct ccgccggaga gcgatgaaac agggctttgc gcacgggctg gatggaacag
      661 gaaatcttcc aaatagctca tgacgtgttt tttcttaccg ccgacttcaa cgtaatcgtt
      721 tccctgtccg agggtgtaaa acacggtgtc gttttcattc aacgcgctgc ggaactggtc
      781 gaaataggcg acttcctgct tactgccgat acggattctg ccgtaggtcg gctgcaattc
      841 gcccaaaatc agcttaagga aggtggtttt gccgatgccg ttggggccga ttaggccgat
      901 tttgtcgccg cgctgcaaga tagcggagaa tttgtccata atgactttgc cgccataggc
      961 aaacgaagcg tgttccaatt cggcgatgat tttgccactt ttctcaccgc tatcgagctt
     1021 gaagttgact tgtccctgta cgttgcggcg ttctgcacgc tggcggcgca gctcttccaa
     1081 acggcgcacg cggccttcgt tgcgggtacg gcgcgcttcg atgcctttgc gtatccatgc
     1141 ttcttcctgt gcgtggaatt tgtcaaagag gcggttgtgt tccgcttcga ctgccaactc
     1201 ttgcgctttt ttctcgctgt atttagagaa cgagccggga taggaacgca aaataccgcg
     1261 atcgagttcg acaatccgcg tggcgatatt gtccaaaaaa cggcggtcgt gggtaatcac
     1321 aaccaagctg ccttcaaacg ctttgagcag attttccagc caaataatcg cgtcgatatc
     1381 caaatggttg gtcggctcgt ccagcagcaa tacgtcgggc ttttgcaccc aagcctgagc
     1441 caaggcgacg cgctttttct gaccgccgga aaggttgccg attttttcat tttccggcaa
     1501 accgagttcc cccaaagtct gcttgactgc cgcatccagt ttccagccgt ccttcgcttc
     1561 gatttcaagt tgcaattcgt tgagttcttt caacaaagcc tcactcgaac cattttccaa
     1621 ctcatggctg acatgatgat aacggcgcaa taaatcacga atttcgccca aaccttcggc
     1681 aacggtatca aatacggttg cgtccttatc aaaaaaggat tcctgcggta cataaacgat
     1741 tttgaggttg ttttgaacaa taatctgccc gtcgtcgagc ttttgcaaac cggcgaggat
     1801 ttttaaaaac gaagacttgc ctgcgccgtt gcgtccgatt aagccgactt tttcgccgct
     1861 gtcgagttga aaagaagttt tgtcgagcaa ggcaacgtgg ccgatggcaa aagaagcgtt
     1921 ttctacagat aatatattca tgatacaaat tctcaacagt taccgtttgg attttaccgc
     1981 aagtttggcg cgggcaattt caaccgcacc cggcaggacg gaaacaataa tgatgccgcc
     2041 catcaccaag cccagattgt tttttacgac ggggaagttg gcaaagaaat agcccgcgta
     2101 agaaaacagg ataacccaca acaagccacc gatgatgttg tagcggataa atttggcata
     2161 gtgcattttc cccataccgg cgacgaaggg ggcgaaggtg cggacgatgg gcataaaacg
     2221 ggcaatgatg atggttttgc cgccgtgttt ttcgtaaaaa cggtgggttt tatcgagata
     2281 ttcacgtcgg aagattttag aatcggggtt ggcgaacagc ctgccgccga aatatttgcc
     2341 gacggtaaaa ttgagcgcgt cgccgagtat ggcggcaagg cttaataatg caaccatcaa
     2401 atgaatatcc ataccgccca gcgcggcaat cccgccggcg gcaaacagca gcgaatcgcc
     2461 gggcagtaag ggcgtaacaa tcaggccggt ttcgcaaaaa acaatcaaaa acagaatcgc
     2521 ataaatccac acaccgtatt gcgccgacag cgcgagcagg tgttggtcga tatggaggat
     2581 gaagtcgatg gcggaggcaa gcacggcgcg ttcctaaaaa aaacaaaccg cgtattttaa
     2641 ccgattggaa aaatgccgtc tgaaaagttt cagacggcat cggctattca aattcatttc
     2701 acgtaaaaac cgcaaaccaa aatagtttgc ggtttggcat ttaaagtgac aatgatgatt
     2761 tcaaatcatc agaattttat gccgacgcgc aagccgtatt cacgaatact ggttttcggg
     2821 atggtgagcg atacgtcgcc actcttggtt gttacactaa actcgccgga ttctttgtaa
     2881 gtgcgttgtt tgtagaacgg ccccgcctcg atgctggcgg attcgcccag ttttttacca
     2941 atatttgcac ccaatccaaa gccccagccg ttggtttttt gattgatgtc ttttttgaga
     3001 ttggtaacgc cggtgtttaa tttatagcgg gaattgaggt caaatttcac ttcagaccaa
     3061 gggttgatat accagccgtt acccagttgg gaaagcaaat ccgcgtgaac tttggctaac
     3121 cacgactgac ggctgctgtg aagcgtatgc ttggtggttt taatgctgtc ttttgaagat
     3181 tcaaaaccca agccggcacc cacacggaaa tttaaagaat cacttaacgt ttgggtgtag
     3241 gtgtagcctg tgtaaagatc gatacggttt tcaggaacgc cggtgggcag ttttacattt
     3301 ttagtcttac ccagcttgtt ctcatctgtt tccaaattaa taatattttt tttgctgcgc
     3361 ccgaaaccgg cttccaagcg gatgccttgg ttggcatcaa aaggaatatc agcacgcacg
     3421 ctgatgtgtt tggcagcttt gtgtttttct ttcaggaaag cacgagttga agaaatggaa
     3481 gagaggtcgg tgtggacggt aaactcatta gcggtttgaa gctcttgtgc agcggcggca
     3541 gtaccggtca gggcaatcat ggcacatgta aaaactgttt ttttcatagt taaaacctct
     3601 aaaatttgga ttgtagtcgg atatggtaac ataacgtaaa taatcgttac gcttacaatt
     3661 atattcttaa gctttcgggg ggggggggat tttacatata ttaataaaaa ttaacaaata
     3721 gttatttgtt tacaacgaat tgttattctc acttggtttt
//
LOCUS       DAWISC010000172         3717 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWISC010000172 DAWISC010000000
VERSION     DAWISC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.172"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(225..548)
                     /locus_tag="WIW87_001807"
     CDS             complement(225..548)
                     /locus_tag="WIW87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ5174926.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(628..1284)
                     /locus_tag="WIW87_001808"
     CDS             complement(628..1284)
                     /locus_tag="WIW87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ5174927.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDVEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1387..1914)
                     /locus_tag="WIW87_001809"
     CDS             complement(1387..1914)
                     /locus_tag="WIW87_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ5174928.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAV"
     gene            2069..2842
                     /locus_tag="WIW87_001810"
     CDS             2069..2842
                     /locus_tag="WIW87_001810"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ5174929.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRIHPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2849..3199
                     /gene="secG"
                     /locus_tag="WIW87_001811"
     CDS             2849..3199
                     /gene="secG"
                     /locus_tag="WIW87_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ5174930.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3213..3298
                     /locus_tag="WIW87_001812"
     tRNA            3213..3298
                     /locus_tag="WIW87_001812"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3247..3249,aa:Leu,seq:gag)
BASE COUNT         1012 a         1171 c          790 g          744 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgcccgcctc gtcctagccc
       61 tctcccacgg gagaggggat tggggtgcag gctgccttta aggttcaggc aaattttaac
      121 ttcgttgata ccgcgcttta gcttcgcaga agctgcactt tcagacgacc ttttggaaca
      181 ccacaggtac acgcatttaa ggaatgccgt ctgaaatgcc tgcctcaata acgcatcatg
      241 ttgccgtcaa tctcggccgc ccatgcatcg atgccgccct gaaggttgta caggttttca
      301 aaacccgcgt ccgccaaata catcgccgta tgcaggctgc ggataccgtg gtggcaatac
      361 accacaagcg gcacatcatc cggcagctcg ttctgccgca gcggaatcag attcatcggg
      421 atatgcagcg catttggcag cgaacaaacc gccgtttctt catccgtacg cacgtccaac
      481 aaacaaaaca tccgcccttc gtccatccac gctttcaatt ccgcgggccc aagttgcaca
      541 atatccatcg caccccccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaacccgc
      601 tttcagacgg catgacctgt caacatctta aaaatcgaaa ccgccaaacg gatcggcatc
      661 cttatcatcc aaatgcgcca ccaaggtatc gaacagcacc ttctcttcaa acacatcgcc
      721 cctgcgcgta atcaaaagcg cgcgttgaac aggcttgcga cctacgataa ccaccatgcg
      781 tccgccatct ttcaactgtt ctttcaacac ttcaggcaca aggtttaccg caccgccgac
      841 ataaaccgca tcaaacggcg cacctgcgga aagttcggtc aacccgttgt tttgcacata
      901 atcgatattg tccaaaccca agccgtccaa caccgctttg gcgcggtttt gctgttcgac
      961 atcgatgtcg tccgacacca cacgaccagc caattttgcc aacagcgcgg tcgcatagcc
     1021 cgaacccgtg ccgatttcca aaaccgtatc gtttttcgtc agcttcaagc cctgcgccag
     1081 ccgcgccacg actttcggct cgagcatctt atgaccgttg gcaagcggca gcgccatatc
     1141 cgcatacgcc aaaccctgca agtcctcatc gacaaaaagc tcgcgcggaa tctccgccaa
     1201 agcgtccaac acatcaaaat ccaatacatc ccacggacgg atttgctgtt cgaccatatt
     1261 gaaccgcgct ttttcaaaat ccatttagtg ctccgtcaaa taattgttcc aacaatggca
     1321 ggcattataa aaccacactc ccgccgcgcc aacttcggca cgcgccgaca gcccgttttg
     1381 tcagtctcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagttc
     1441 gcccaaatac aaagcatccg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gtgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtt gacatcctta acaaccaccg tttcaccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgcctcgtca gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacagcct gcccgaaaca acattccaaa ccgcccggcc ggccgcttca
     1981 agttgcgaac ccgccgcata tccactaaac ttcacgttgc accgcgccac acgcggcaga
     2041 caaaaaaaca cgacacggag caaaaaagat gtatcgccaa atcggaatgt gggatcaaaa
     2101 atgggtcatc ggcaactgga aaatgaacgg ccggctccaa aacaacaacg cactgatgca
     2161 ccgcttccgc atccacccca ccgccgaacg cgtcctcatc ggactcgccg ccccgaccgt
     2221 ttacctgctg caactgcaca acgccatgca aatcgtttta aacaaccgca tcctcacctg
     2281 cgcccaagac gtgagccgct tccccaataa cggcgcgtac accggcgaag tgtccgccga
     2341 aatgctcgcc gacaccggca cagacatcgt cctcatcgga cactccgaac gcagccttta
     2401 tttcggcgaa aaaaacgaaa tccaacgccg caaaatggaa aacgtcctca acgtcggact
     2461 catcccgtta ttgtgcgtcg gcgaaagcct cgaagagcgc gaagccggca aagaacacga
     2521 agtcatcgcc catcagcttt ccatcctgca agggctggat accaaaaaca tcgccgtcgc
     2581 ctacgaaccc gtctgggcga tcggcaccgg caaagtcgcc accgtcgaac agattgccga
     2641 tatgcacgca ttcatctaca aagaaatctt gtctttgtgc ggaagcgatg ttaaaatccg
     2701 cgtcctttac ggcggaagtg tgaaagcgga caacgcggcc gacatcttcg cagtacctta
     2761 tgtggacggc gcactcgtcg gcggcgcgtc attgtcgtac gactccttta ccgccatcat
     2821 cagtgccgca caaaatgcgt agaaaaatat ggaagccttc aaaaccctaa tttggattgt
     2881 taatataatt tccgctttgg ccgtcatcgt gttagtattg ctccaacacg gcaaaggcgc
     2941 ggatgccggc gcgactttcg gatcgggaag cggcagcgcg caaggcgtat tcggctctgc
     3001 cggcaacgct aacttcctca gccgctcgac cgccgttgca gcaacatttt tctttgcaac
     3061 ctgcatggct atggtgtata ttcacaccca cacgacaaaa cacggtttgg acttcagcaa
     3121 cgtacaacaa actcagcaag cacccaaacc cgtaagcaat accgaacctt ctgcccctgt
     3181 tcctcagcag cagaaataac agtttttcaa atgccgacat ggtgaaattg gtagacacgc
     3241 tatcttgagg gggtagtggc cgtaggctgt gcgagttcaa atctcgctgt cggcaccaac
     3301 acacaaaaac gcctgaaaat ttcaggcgtt ttttgttcaa tcccatccaa cgctgtcaac
     3361 caattccaat caatacaagg atttcaggct gattgttatc ctgccgtccc ccttcctgac
     3421 agtgcaatcc cgtccaatcc gccctaattg aagtaaccta aaatttacgg tatcttttgc
     3481 ggtatctgaa aaatacctcg aaaaaatacc gcaaaaataa agctgaacga ccgccaaatc
     3541 aggaatgcca agcggaaaag agcttgcggg gaatactgcc aagacgtagg gaacaagggg
     3601 gaaaccgtcc aagatgcagg gcggtttttt ttgggttttt ggaaaaaacc tatactagga
     3661 agcgataccc ttagttgtta ccttgttacc ggggaaaagt tagataaata agcatat
//
LOCUS       DAWISC010000173         3709 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWISC010000173 DAWISC010000000
VERSION     DAWISC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.173"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..245)
                     /locus_tag="WIW87_001813"
                     /pseudo
     CDS             complement(1..245)
                     /locus_tag="WIW87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(729..835)
                     /locus_tag="WIW87_001814"
                     /pseudo
     CDS             complement(729..835)
                     /locus_tag="WIW87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1254..2024)
                     /gene="xth"
                     /locus_tag="WIW87_001815"
     CDS             complement(1254..2024)
                     /gene="xth"
                     /locus_tag="WIW87_001815"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ5174931.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2084..2365)
                     /locus_tag="WIW87_001816"
     CDS             complement(2084..2365)
                     /locus_tag="WIW87_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ5174932.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2457..2744)
                     /locus_tag="WIW87_001817"
     CDS             complement(2457..2744)
                     /locus_tag="WIW87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174933.1"
                     /translation="MSGKEPLNPNPVIASGRKCRLKDSSDGIFRKGRDALEKTAFQAG
                     ILFARQRGGIGRAGKRTGGGSFMPSESPPLRLFAKKVGKGTYNPVQSSINI"
     gene            complement(2772..3653)
                     /gene="nadC"
                     /locus_tag="WIW87_001818"
     CDS             complement(2772..3653)
                     /gene="nadC"
                     /locus_tag="WIW87_001818"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ5174934.1"
                     /translation="MPSEKTLFPLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
BASE COUNT          892 a          887 c          937 g          993 t
ORIGIN      
        1 ggggcaatgc cggccaaacc cgctatccgt ttgtgcgaca gccgcctcaa ttcgggcagc
       61 atcgccatca gcgtagccgt cgttatcgaa ccgatgcctt tgatttgttc tgccacttgg
      121 gctttgccgt caaaatgcgt gtgggtgtgg tcgtcgattt gtttgtccaa ttcgtcaatc
      181 agccggtcaa aatgggcaat cagttgtttg acgcttccga cttgcgtttc atgaaactga
      241 tgcagatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
      301 tagtacggca acgctgtact ggtttaaatt taatccacta tattttgtaa tagctgccat
      361 tatgcctact aggatatata ccttctatat aatccttatt taaataaaaa cccttataaa
      421 aatctgtgtt atctgtcggg attgaaaaaa agattttatc tttctcatca ggtttatatt
      481 tgctatcaat atcaaaaaga gaataaggat tacaatgagt tcgacatact aggtaaaaat
      541 ttatatcttg gatcatgaaa tattcgatat ttatcaggat cgaaagatgc taaaaattca
      601 tcaatattgg tactggatat tttgtcatca ctaataaaca tcgctagaga tgggcgactt
      661 tgattttcca tttgttgatt agtaaaggat ataaatacta acttgccatt gtcaaaccac
      721 atagatttat atagtggatt aaatttaaac cagtacgtcg ttgcctcgcc ttgccgtact
      781 atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatagttat
      841 tgcctttggg agaatcaatt ctgacagcac ataacataga tttatcttta cagtcgttat
      901 attgagtttc atcaaactca actgcatggc acaataaagg caataggaaa aacagggtta
      961 ttaatttatt actatatata agacggcttt ctatttattc gaaaataaat ctagttttca
     1021 atatttgatt ttgttttgga ttagtgtaca taataaattt tactgctcca aaatctgata
     1081 ttaaccctat gtatttcata aatttttcag gaaaataggc tgtgtttgaa caatccgccg
     1141 atttcggctg tttgccgggg aaaggcaatt atttttcagt cagatagaaa gctatcctat
     1201 tgaataaatt gaagataagg atgcttgtca acgccatatc aaaacacggt cttttaccaa
     1261 tcgaattctg ccgtcaccgg cgcgtggtcg ctcggacgct ccagcgcgcg cgtctccaaa
     1321 tcgacgcgga catccttcaa cgccgccgcc atcgcaggcg acaccaaaat atggtcgata
     1381 cgcaggccca gtttgcgttg gaacatcgcg ccgcgatagt cgaaccaggt atagaacgcg
     1441 ccttcgggat ggacttggcg caggctgtcg gtcagtccca aatccagcag gttttgaaac
     1501 cactgccgtt cgacggacga acagtggatt ttttcgtgcc atttttcagg gtcgtaacag
     1561 tccgcatcgg caggcgcgat attgaaatcg cccagcaaca ccagtttgcc gtggcgggtc
     1621 atttcatcgc ggacaaactc cgtcagtgcg gcaaaccact gttccttata tttgaatttg
     1681 gggctgtcca aagcctcgcc gttgacgcaa tagacattga tgacgcgcac gccgctgacg
     1741 gttgccgcaa tcacgcgccg ttgcggatcg tccggcagtg cgggcaaacc gaaatgcacg
     1801 tcctgcggca cgctgcggct gacgattgcc acgccgttgt aggttttctg cccgctccaa
     1861 acacagtgcc agcccatcat ttgcaaagcg gcggccggaa atttgtcctg atcgagtttg
     1921 agttcctgca aaaccaaaat atcgggcgga ttgtcggcaa gcaggttttg cacctgcggc
     1981 agccgcacat tgagcgaatt gacattccaa gtggtgattt tcataacatt tccgaaaaat
     2041 gctgtctgaa acgttcagac ggcaaaagga aaggattcta acactatgcc acgcgtttgt
     2101 tttccaaatc gcgaaccgtg tgcagaatcg ccgccgcttc ttcggaaacc aggcggtatt
     2161 cgataatgcg gtggtaacgc gtaaaatcga ctagaccttc cacgcgcagg cggttcaaat
     2221 ggttggaaac tgcggtggcc ggcagggata aggattttgc cagttcggcg atattgcgtt
     2281 cgctgtccaa caattgaatc agtatcgcca tacgttcggg atgcgccata agtttgagta
     2341 tggtgtggag cggtattgtg ttcatcggta ttcctaaaac ctttgccgcc cgccaagcgg
     2401 attgacgggc ggcagatatg gatgattgtg attatcttta taaatcgtat tataaatcaa
     2461 atatttatgg atgattgtac aggattgtat gttccttttc ccactttttt tgcaaacaag
     2521 cgtaaaggcg ggctttcaga cggcataaaa ctgccgcccc ccgtcctttt tcctgcccta
     2581 ccgatgccgc cgcgctgtcg ggcaaacaaa atacctgcct gaaatgctgt tttttctaaa
     2641 gcatcacgac ctttacgaaa aatgccgtct gaagaatctt tcagacggca tttcctccct
     2701 gaggcaataa ccggattagg gttaagcggt tccttgcctg acatatcaga cagccgcccg
     2761 caccctaaaa ctcacgccac gaaatccaaa cctatgtcca acgaacggct gctgtgggtc
     2821 agatagccga gggcgatgcc gtccactccg gtttgcgcca cgcgcttcag gcggtcgaag
     2881 ccgatgccgc ccgatgcttc gcaatagatg gtgtgggggt gggcggtttg cgtgtggcag
     2941 cggtttgccg cttctttcag ggtttcgtcg tccatgttat ccagcaaaat ccgttccgcg
     3001 cccgctgcga tggcttcgtc cagttgtgcc aacgtatcca cttcgatttc cacgcaggtc
     3061 aatgctccga cagcctgttt tgcctgctgc acggcttggg cgatgctgcc gcaataggcg
     3121 aggtggttgt ctttgatgag cacggcgtcg tccaaaccca tgcggtggtt cacaccgccg
     3181 cctgccctga cggcgtattt ttgcaggaca cgcagcaggg ggatggtttt gcggctgcac
     3241 acgatgtctg taccgtattc ggcgacttcg gcaacggcac gcgcggtggc ggtggcgatg
     3301 ccgcttaagt gcgtgaggta gttgagcgcg gtgcgttcgg cggcgagcag cgcgcgggcg
     3361 ttgccttcga cggcggcaag cgtctgacct gcgcggacgg cttgcccgtc tcggatttcg
     3421 gcttggaagc ggacggacgg atccatcgtc tgaaaggcga gacgcgccaa gtccatgccg
     3481 gcgataacgc cgtcttcgcg gctgacaagg aagagtttgg cggttttgtc gggggcgatg
     3541 acggcggcgg acgtaatatc gccgcgcctg cccaagtctt cgctcaaggc ttgttctact
     3601 atggggcgca acagggtgtc gggcagggga aagagggttt tttcagacgg catggggttt
     3661 cctttgttga aaaagattga tgttgcaccg atggggcaaa acttcattt
//
LOCUS       DAWISC010000174         3708 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWISC010000174 DAWISC010000000
VERSION     DAWISC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.174"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(153..398)
                     /locus_tag="WIW87_001819"
     CDS             complement(153..398)
                     /locus_tag="WIW87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174935.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="WIW87_001820"
     CDS             510..938
                     /locus_tag="WIW87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ5174936.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="WIW87_001821"
     CDS             950..1426
                     /locus_tag="WIW87_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ5174937.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLTDAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="WIW87_001822"
     CDS             complement(1776..2264)
                     /locus_tag="WIW87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ5174938.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WIW87_001823"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WIW87_001823"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ5174939.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          838 a          972 c          871 g         1027 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaaccgct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgcattgc
     1081 cggaaaacag gcgtggctga cgctgaaagg ctatatttcg gaaatcagcc gcagtgagtt
     1141 tgaatacgaa atcccgctta ccgatgcgga aaagatgatg gaaacgatgt gcccgtttaa
     1201 aatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaaacg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagcgcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc cgccccgatg
     2221 cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cgacatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctctt gccgtacact tccggcttgt cctgtctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaatg tcttgtgcca cttccatctg ccgagtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt tccgcatcat aattcggctc tttgccgccg cgcaaaatga catgacagtc
     2701 gggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg ctatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatgggttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttta tagattttaa atttcatatt tttattattt
     3541 tagaaatttt atttcaaata aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 tggacaaacc tgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 ccccgctgcc gattccgcct tcacatccgg caaaatttca tacacaag
//
LOCUS       DAWISC010000175         3694 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWISC010000175 DAWISC010000000
VERSION     DAWISC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.175"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            60..689
                     /locus_tag="WIW87_001824"
     CDS             60..689
                     /locus_tag="WIW87_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ5174940.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3242
                     /locus_tag="WIW87_001825"
     CDS             864..3242
                     /locus_tag="WIW87_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEZ5174941.1"
                     /translation="MKPLQMLPIAALLGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAIRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPTNYTTWETEYHKKEVGEIYNRSMDTTFKRITLRMDSHPLQLGGGGRHR
                     LSFKTFAGQRDFENLNRDDYYFSGRVVRTTNSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNADCHACDKTPPAANTYKGWSGFVGLAAQLSQTWRLGYDVTS
                     GFRVPNASEVYFTYNHGSGTWKPNPNLKAERSTTHTLSLQGRGDKGTLDANLYQSNYR
                     NFLSEEQNLTVSGTPGCTEEDAYYYRCSDPYKEKLDWQMKNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAG
                     VYNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(3450..3572)
                     /locus_tag="WIW87_001826"
                     /pseudo
     CDS             complement(3450..3572)
                     /locus_tag="WIW87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
BASE COUNT          996 a          938 c          945 g          815 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga ccttgcagat ttgggcgaag aaccttacaa attcgacaaa gaattaccgc
      361 atgaaaccgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcgttttt gttcaaacac gcgcaaaaac tcgattacaa cggcgaacac ggtgcgcgcc
      481 acctcgcacc gcatcccgac gggcgcggca aacactggcg cgccttcgtc gagcatctga
      541 acgctttgaa cctgactccc gaagccgaag cggaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagcac
      661 cggaaggaat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagagac ctttaattac acggcgcggc tttgtttgca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgc
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggtca gcgcaatgcg cctgcggctg
     1021 tggaacgtgt caaccttaac cgtatcaaac aagaaatgat acgcgacaat aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg tcatcaaaaa ggctttgcca
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg ttagtattga cggcgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgacatcg taaaaggggc ggactctttc aataccggca
     1321 gcggcgcctt gggcggcggt gtgaattacc aaaccctgca aggacgtgac ttactgttgc
     1381 ctgaacggca gttcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcgg gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgacgaa ttacaccaca tgggaaaccg
     1921 agtaccataa aaaggaagtt ggcgaaatct ataaccgcag catggataca accttcaaac
     1981 gtattacgct gcgtatggac agccatccgt tgcaactcgg gggggggggg cgacaccgcc
     2041 tgtcgttcaa aacctttgcc gggcagcgtg attttgaaaa cttaaaccgc gacgattact
     2101 acttcagcgg ccgtgttgtt cgaaccacca acagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcgctgtcc gaccaaatcc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgaccacacc aaaatgacgc ctcaggaatt gaatgccgac tgtcatgctt
     2281 gtgacaaaac accgcctgca gccaacactt ataaaggctg gagcggattt gtcggcttgg
     2341 cggcgcagct gagccaaaca tggcgtttgg gttacgatgt gacctcaggt ttccgcgtgc
     2401 cgaatgcgtc tgaagtgtat ttcacttaca accacggttc gggcacttgg aagcctaatc
     2461 ctaatttgaa ggcagaacgc agcaccaccc acaccctgtc cttgcagggg cgcggcgaca
     2521 aagggacact ggatgccaac ctgtatcaaa gcaattaccg aaacttcctg tcggaagagc
     2581 agaatctgac tgtcagcggc acacccggct gtactgagga ggatgcttac tactatagat
     2641 gcagcgaccc ctacaaagaa aaactggatt ggcagatgaa aaatatcgac aaggccagaa
     2701 tccgcggtat cgagctgaca ggccgtctga atgtggacaa agtagcgtct tttgttcctg
     2761 agggttggaa actgttcggc tcgctgggtt atgcgaaaag caaactgtcg ggcgacaaca
     2821 gcctgctgtc cacacagccg ctgaaagtga ttgccggtat cgactatgaa agtccgagcg
     2881 aaaaatgggg cgtattctcc cgcctgacct atctaggcgc gaaaaaggtc aaagacgcgc
     2941 aatacaccgt ttatgaaaac aagggctggg gtacgccttt gcagaaaaag gtaaaagatt
     3001 acccgtggct gaacaagtcg gcttatgtgt ttgatatgta cggcttctac aaaccggcta
     3061 aaaacctgac tttgcgtgca ggcgtgtaca acctgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggtttatat agctacagca ccaccaatgc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaggccgca attacgccgt atcgctggaa tggaagtttt
     3241 aatctggtat tattgaatta atcgccttgt tgaaaattaa agccgtccga attgtgttca
     3301 agaattcatt cggacggctt tttaccgtaa atctgtgtga tggggtttta tattgataca
     3361 gtatccgaac tcaggcaggg ggtaatgttg caggattaaa aatgccgtct gaaaaatgtt
     3421 tcagacggca ttttttggtt gccgatatgt taacggacga cgttggcgcg catgaccacg
     3481 cggacgtgga acgagcctgc ctgacagtcg tattgtccgc cttgcagcgg ctgcttgtcg
     3541 aagtagctgt ggaagccggt aacgccgcgt ccgcaagggt ggcggcagat gccgcattgt
     3601 gtttataatg ccgtgtcaga cgtttgattt atgggaaaat aatgatgctt accaatcagg
     3661 aagtaaccgg tgtcaaactg ggataccgca aagc
//
LOCUS       DAWISC010000176         3638 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWISC010000176 DAWISC010000000
VERSION     DAWISC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.176"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            262..609
                     /locus_tag="WIW87_001827"
     CDS             262..609
                     /locus_tag="WIW87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ5174942.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPRICI
                     PETLQTQMTHTRI"
     gene            606..3467
                     /locus_tag="WIW87_001828"
     CDS             606..3467
                     /locus_tag="WIW87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ5174943.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          981 a          998 c          975 g          684 t
ORIGIN      
        1 agggaaattc aaattaagtt agaattatcc ctatgagaaa aagccgtcta agccggtata
       61 aacaaaataa actcattgag ctatttgtcg aaagttcaaa tttccatttt aaaacaatta
      121 gtaaaatcga gtttatccta gttgtccaag acaaccccta taataatata attcaaaata
      181 taaaaacggg ttacatctaa acattacgga atttttattc cctcgcctga attctattgt
      241 cagattcaag gagacctcat catgcgaacg accccaacct tccctacaaa aactttcaaa
      301 ccggctgcca tggcgttagc tgttgcaaca acactttctg cctgcttagg cggcggcggc
      361 ggcacttctg cgcccgactt caatgcaggc ggcaccggta tcggcagcaa cagcagagca
      421 acaacagcga aatcagcagc agtatcttac gccggtatca agaacgaaat gtgcaaagac
      481 agaagcatgc tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc
      541 aatgcccccc ccccccgaat ctgcataccg gagactttac aaacccaaat gacgcataca
      601 agaatttgat caacctcaaa cctgcaattg aagcaggcta tacaggacgc ggggtagagg
      661 taggtatcgt cgatacaggc gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca
      721 gaaaagaaca cggctataac gaaaattaca aaaactatac ggcgtatatg cggaaggaag
      781 cgcctgaaga cggaggcggt aaagacattg aagcttcttt cgacgatgag gccgttatag
      841 agactgaagc aaagccgacg gatatccgcc acgtaaaaga aatcggacac atcgatgtgg
      901 tctcccatat tattggcggg cgttccgtgg acggcagacc tgcaggcggt attgcgcccg
      961 atgcgacgct acacataatg aatacgcatg atggaaccaa gaacgaaata atgtctgcag
     1021 ccatccgcaa tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt
     1081 ttggaacaac atcgagggca ggcactgccg accatttcca aatagccaat tcggaggagc
     1141 agtaccgcca agcgttgctc gcctattccg gcggtgataa aacagacgag ggtatccgcc
     1201 tgatgcaaca gagcgattac ggcaacttgt cctaccacat ccgtaataaa aacatgcttt
     1261 tcattttttc ggcaagcaat gacgcacaag ctcagcccaa cacactgacc ctattgccat
     1321 tttatgaaaa agatgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag
     1381 aaaagttcaa tggctccaac cattgcggaa ttactgccat gtggtgccta tcggcaccct
     1441 atgaagcaag cgtccgtttc acccgtacaa acccgattca aattgccgga acatcctttt
     1501 ccgcacccat cgtaaccggc acggcggctc tgctgctgca gaaatacccg tggatgagca
     1561 acgacaacct gcgtaccacg ctgctgacaa cggctcagga catcggtgca gtcggcgtgg
     1621 acagcaagtt cggctgggga ctgctggatg cgggtaaggc catgaacgga cccgcgtcct
     1681 ttccgttcgg cgactttacc gccgatacga aaggtacatc cgatattgcc tactccttcc
     1741 gtaacgacat ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc
     1801 acggcaacaa cacctatacg ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg
     1861 gcaacaacaa atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg
     1921 catccggcgg tagcctgaac agcgacggca ttgtctatct ggcagatacc gaccgatccg
     1981 gcgcaaacga aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt
     2041 acacacgttt gggcaaactg ctgaaagtgg acggtacggc gatgaccggc ggcaagctgt
     2101 acatgtcggc acgcggcaaa ggggcaggct atctcaaccg taccggacaa cgtgttccct
     2161 tcctgagtgc cgccaaaatc gggcgggatt attctttctt cacaaacatc gaaaccgacg
     2221 gtggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc
     2281 tgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg gcacattccg
     2341 cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa aacctgatgg
     2401 tcgaactgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact gcggccgccg
     2461 accgcacaga tatgccgggc atccgcccct acggcgcaac tttccgcgca gcggcagccg
     2521 tacagcatgc gaatgccgcc gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct
     2581 atgccgacag taccgccgcc catgccgata tgcagggacg ccgcctgaaa gccgtatcgg
     2641 acgggttgga ccacaacggc acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg
     2701 gaacgtggga acagggcggt gttgaaggca aaatgcgcgg cagtacccaa accgtcggca
     2761 ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcatg ggacgcagca
     2821 catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac
     2881 ggcacgatgc gggcgatatc ggctatctca aaggcctgtt ctcctacgga cgctacaaaa
     2941 acagcatcag ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc
     3001 tgatgcagct gggcgcactg ggcggtgtca acgttccgtt tgccgcaacg ggagatttga
     3061 cggtcgaagg cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca
     3121 gtgctttggg ctggagcggc aacagcctca ctgaaggcac gctggtcgga ctcgcgggtc
     3181 tgaagctgtc gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac
     3241 gcgacctgaa cggacgcgac tacacggtaa cgggcggctt taccggcgcg actgcagcaa
     3301 ccggcaagac gggggcacgc aatatgccgc acacccgtct ggttgccggc ctgggcgcgg
     3361 atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacgcc ggttccaaac
     3421 agtacggcaa ccacagcgga cgagtcggcg taggctaccg gttctgacgg acaggaagca
     3481 gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa ccctattcca
     3541 attgcctgct tctattgttt caagacttct tccaaagatt cggcattaat cagatgtata
     3601 gcggattaac aaaaatcagg acaaggcggc gaagccgc
//
LOCUS       DAWISC010000177         3562 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWISC010000177 DAWISC010000000
VERSION     DAWISC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.177"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..976)
                     /locus_tag="WIW87_001829"
     CDS             complement(122..976)
                     /locus_tag="WIW87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ5174944.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1100..1828)
                     /gene="rpsB"
                     /locus_tag="WIW87_001830"
     CDS             complement(1100..1828)
                     /gene="rpsB"
                     /locus_tag="WIW87_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ5174945.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2005..2238)
                     /locus_tag="WIW87_001831"
     CDS             complement(2005..2238)
                     /locus_tag="WIW87_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174946.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLFFWAVLPTVFTVFV
                     VTIRRRNRLMRQQEQAEFEQQRAQRQKDSGTKP"
     gene            2316..2897
                     /locus_tag="WIW87_001832"
     CDS             2316..2897
                     /locus_tag="WIW87_001832"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ5174947.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPVEAHDRSLDGFVSEAGILCF"
     gene            2958..3548
                     /locus_tag="WIW87_001833"
     CDS             2958..3548
                     /locus_tag="WIW87_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5174948.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDSAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          785 a          823 c         1024 g          930 t
ORIGIN      
        1 tgttttgttt tcagacggcc tttatattcg gtcgtcttaa aataggtacc gcaaacggtt
       61 ttctcaaaaa aaagcaccct gattcgtttg gaatccaggt gctttctttt tcataagtgc
      121 cttacacttt ggcggctgcg gctacttcgg ctgcgtagtc gacaacggct ttctcgatac
      181 cgtcgcctac tttgtagcgg acgaagctga ccacttcagt gccgttttct ttggcgaatt
      241 gggcaacagt ttgatcgggg ttcatcacga atgcttggcc gttcagagtg atttcagcca
      301 agaatttgcg gatacggcct tcaaccattt tagcggcgat gtcggcaggt ttgccggaag
      361 cgatggcttg ttcagtgtag atgtggcgtt ctttttcaac ggtttcagca tctacttcgg
      421 cttcgcttac gcattgtggt ttagcggcaa caatgtgcat accgattttg cgtgctacgt
      481 cttcagagcc tttgtactca accaatacgc cttcggtagc caatgcgccg tggatgtagg
      541 caaccagttg gttggcagtg tcgatcactt ggaagcgacg gacagacatg ttctcgccca
      601 atttggcgat gatggctttg cgttctgctt caaccagttc gctcagttct tcaacagaag
      661 ccggtttttt ctcggcagca gttttagcaa cgaagttggc gaattctacg aagccggcat
      721 ctttagcaac gaagtcggtt tcgcagttta cttcaaccaa tgcaccgaca ttgccgttga
      781 tcgcgtaagc caatacgcct tcggcagcag tacggccggc cagtttaccg gctttcgcac
      841 cagatttaat acgcaggatt tcttcggctt tgtcgaagtt gccttcagct tcaaccaagg
      901 cttttttgca ttccatcatg cccaggccgg tagcggcgcg caggtcggca accatttttg
      961 cagtaatttc tgccattttg aatctcctag atttttggga acacggccat tgccgtgttt
     1021 ggaaatagtg gccgtctgaa acggaattca gaacgggatt tgagaaaagg ggcataacgc
     1081 ccctcttctg tgtaccgaat tactcggcag cagcttcttg ggcagcggct acagtttctt
     1141 gcagcgcttg gtttttgcct tccaaaactg cgtcagcgat gccgcggcag tacaggcgga
     1201 tggctttggc ggagtcatcg ttaccgggga taacgtattt cacgccgtca gggctgttgt
     1261 tggtatcgac tacggcgata acagggatgc ccaatttttc agcctcaacc agagtacctt
     1321 tttggtagcc ggtatcgata acgaaaatcg cgtcaggcag gcctttcatg tttttgatac
     1381 cgcccaaaga acgttccagt ttttcaacgt cgcgttgcat ttccagaatt tcttttttgt
     1441 tgaaaccgcc ttcggcagca ttttccaagg ctgcggtttt ttcttccagg cgtttgatgg
     1501 attgcttaac ggttttgtag ttagtcagca taccacccaa ccagcggtaa tcaacgaaag
     1561 gcataccggc acgggtcgct tcttcgcgga tgatgtcgcg ggcttggcgt ttggtaccta
     1621 cgaacaatac tgtacctttg ttggcaacca gacgacgtac ggcttcttgc gcctcttgga
     1681 acatcggcag ggttttttcc aagtttacga tatggatttt gttgcgcgcg ccgaaaatgt
     1741 attgtgccat tttcggattc cagaaacggg tttggtggcc gaagtggaca ccggcttcaa
     1801 tcatctgacg catagtaatt tgagacattt tgatttcctt gaaagggtta agggcacaca
     1861 ttcaatcgca tagaacttga taggcgatgc ctattgtcaa gcacccttcg gcgaaagtgg
     1921 gcataagttt taaaaaataa aagagtgttt ccgaaatgga aaactgacgg attatagcgg
     1981 ataagaggcc gtctgaaaag ggattcaggg ttttgtgccg ctgtcttttt gccgttgtgc
     2041 gcgctgctgt tcgaattcag cctgttcctg ctgcctcatc aggcggttgc ggcggcggat
     2101 ggtcacgacg aagaccgtaa acacggtggg caacactgcc caaaaaaata aataaatcaa
     2161 cgcgcgcgca atactcggct gcgcggcgga aaacatcacg gcaacaaaaa gatagccgat
     2221 ggcgacaatg tgccacattg tgtgcgtcct gtttgaatgt taataaaatc gttataattg
     2281 caacattcta ccgcaatccc gaaaggccgt ctgaaatgag gaacgaggaa aaacacgccc
     2341 tgcgccgcga attacgcggg cggcgttcgc aaatggggtg ggacgtgcgg gcggcggcaa
     2401 cggtaaaaat taaccgcctg ctcaaacgtt atatcaagca cggtcggaaa atcggcgtgt
     2461 attggccgat gggcaaggaa ttgcgtttgg acggctttgt ccgcgcggcg caaaaacgcg
     2521 gcgcaaaact ttatctgcct tatatcgaac cgcgttcgcg gcggatgtgg tttacgccgt
     2581 atcctgaaag cggaatggaa cgggagcgca tacggggcag ggcgaggttg aacgtgccgc
     2641 agtttgcagg gcgcaaaatc cgcgtgcacg gtttgtcggt attgctcgtt ccgcttgtcg
     2701 gcatagaccg cgaaggctac cgtttggggc aggcaggcgg ctattacgat gcgacgcttt
     2761 cagcgatgaa ataccgtttg caggcaaaaa ccgtgggcgt gggctttgcc tgccagttgg
     2821 tggacagact gccggtcgag gcgcacgacc ggtctttgga cggttttgtg tcggaggcgg
     2881 ggatattgtg tttttagccg ttcagacggc attccgggcg gaaatgccgt ctgaaggcgg
     2941 aatattggga gaaaaggatg tcggaaaaga taatactgcc ggtcttgtcg ttgggcggcg
     3001 ttcgtctcga accgttggat gtgcatcatg aaacgggttt gcgcgaggcg gtttgcgatg
     3061 gggaggtttg gaagctgggg gtaacgtccg cgccccatcc ggatagggtg gcagactata
     3121 tcgggacagc attggcaacg cgtttggcgt ttgcggttgt cgatgaagag gcgggcaggg
     3181 tggtcgggac aacggcgtat tatcattttg aaccgcagat tccgcgtttg gatatcggat
     3241 tcacatggta tgcggcatcg gcacggcgga cacggataaa cacctgttgc aaaatcatgc
     3301 tgttggattc cgcatttgat gttttggctt gccgttgcgt gggatggcgc acggatattc
     3361 tgaaccttgc ctcgcaacgt gccatcgaac ggctgggtgc ggaaaaagac ggcgtgctgc
     3421 gtatgcatat gctccgtaaa gacggcagtg tgcgcgatac ggtcgtgtac agtatgctga
     3481 gggaagattg gtgcaaaaac agggaaatcc tgaccggcag gttggcaggg tatggcgtgc
     3541 aggtgtgagg cagtattttc aa
//
LOCUS       DAWISC010000178         3555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWISC010000178 DAWISC010000000
VERSION     DAWISC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.178"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            61..332
                     /locus_tag="WIW87_001834"
                     /pseudo
     CDS             61..332
                     /locus_tag="WIW87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            372..1406
                     /locus_tag="WIW87_001835"
     CDS             372..1406
                     /locus_tag="WIW87_001835"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ5174949.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1612..2193
                     /locus_tag="WIW87_001836"
     CDS             1612..2193
                     /locus_tag="WIW87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ5174950.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2262..2444
                     /locus_tag="WIW87_001837"
     CDS             2262..2444
                     /locus_tag="WIW87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ5174951.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            2441..3202
                     /gene="kdsB"
                     /locus_tag="WIW87_001838"
     CDS             2441..3202
                     /gene="kdsB"
                     /locus_tag="WIW87_001838"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ5174952.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEASVALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAIRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3225..>3555
                     /locus_tag="WIW87_001839"
     CDS             3225..>3555
                     /locus_tag="WIW87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ5174953.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EASGEFWFRMAETLSTLPPNRSLGLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKIT
                     ALNYSGLN"
BASE COUNT          885 a          882 c          987 g          801 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taaatgaaag atactgaaaa
       61 atgaaagaga tgaaacctgt ccgttatcat attggcgata tgcccgagac ttcaaaacaa
      121 accgcctccc gtcatgacga cagggcagtg ggtgttgacg atgattgttt tcatgattcc
      181 tttggtcaat ttttgtttgg gtgttcggca gaggcaaccc gaaccgcgcc aatttctgta
      241 aagcgcagtt gcttatttac ctgattggtt cgcttatcgg tttggtcttc gcgttgttta
      301 taggtgggtc tgtatcaggt acgcatgatt aatgccccgg gctgattttg cttcgaggat
      361 ttgtatcgaa tatgccgaat tgtttcaaat ttcataccgt tatcgaacgg cattggcaaa
      421 aaccttatcc ggttttgtct tttctgctta agccgctctc cgggctgttt gccaaaattg
      481 cggcaaaacg gcggacggat tttttatcgg gaaaacggca aagcgaaaag ctgcccgtgc
      541 ctgtggtcgt ggtcggcaat attcacgcgg gtgggacggg gaaaacgccg attgttgccg
      601 cgctggtgtc gggtttgcag gaaaagggcg tcaaggtcgg catcatcagc cggggctacg
      661 ggcgcaagag caaggcggtt catgtattga atgctgagag ccgagcggaa gatgcgggcg
      721 atgagccttt gctgctgttc cgcaaaaccg gtgcgccgac ggcggtgggc agcagccgtg
      781 cagaggcagg cagggcgttg ctggcggcgc atcccgacat cggactgatt gtggcggacg
      841 acggtttgca gcattacgcc ctgcggcgag atgtggaaat cgcggtgttt ccggcggcgg
      901 atacggggcg cacggatttg gatttactgc ccaacggcag tttgcgcgaa cctttgttgc
      961 ggctggattc ggtggatgcg gtcgtcgtca gcggcggcaa ggcggatgcg ctgtttaggc
     1021 cgtctgaaaa tatgtttcac agccgtatcg aagcgggacg gatttaccgt ttgaacaatc
     1081 cgtccgaaat actggacaca ggccgtctga aaaatcaaac cgtcgtcgcc gtggcaggta
     1141 ttgccaagcc ggcgcggttt tttgattcgt tgcggaatat gggcattacc gtgaagcgaa
     1201 ccgtcgcgct gcccgaccac gccgacattt cggcggcaga tttgcccgat gcggacgcgg
     1261 tcattattac ggagaaagat gcggtcaaat tttcagacgg catttgcacc gataatgttt
     1321 gggtgttgcc cgtttgtgcg ataatcgaac ctgatttggc ggcgtttgtg ttggagcggt
     1381 tggaagatgt accgaaggcc gtctgaaagc acggtttggg cggagtgatt acggatttga
     1441 ataagaacgc ctcgcgccat cattcccgcg caggcgggaa tctaagtctc gaattttcag
     1501 gaatgcctag gaggctccag aaatcccaaa tctccggatt tccacttgga caggaatgag
     1561 aaaaccggtc gtatttttta tctgcattaa tcattcatta aaggattgaa tattaaactg
     1621 aaaaccttgt tattgccctt cgccacgctg gcattgtgca ccaatgcttt tgccgccccg
     1681 cccagcgacg cgtcgttggc gcgttggctg gatacgcaga attttgaccg ggatatagaa
     1741 aaaaatatga ttgagggctt taatgccgga tttaaaccgt atgcggacaa agcccttgcc
     1801 gaaatgccgg aagcgaaaaa agatcaggcg gcagaagcct ttaaccgtta tcgtgagaat
     1861 gttttgaaag atttgattac gcccgaagtg aaacaggctg tccgcaatac tttattgaag
     1921 aatgcccgtg agatatacac gcaagaagaa attgacggca tgattgcctt ttacggttcg
     1981 cctgtcggtc agtccgtcgt tgccaaaaat ccgcgcttaa tcaagaaatc gatgagtgaa
     2041 atagcggtat cttggactgc attgtcaggg aaaatcgcgc aacatcatct gcccgagttt
     2101 acggaagagt tgcggcgcat catctgcggc ggtaaaaatc ccgatgcggg ctgtaaacaa
     2161 gccggacagg ttgggaaaag gcatcagaaa taaatgatag ccgtctgaaa tattgaagag
     2221 ggcatccgat tgattgaacc atcaaacccg aaagcaaccc tatggaaaaa aaattcttag
     2281 acatcctcgt ctgccccgtt accaaaggca ggctggaata tcatcaggac aaacaggaat
     2341 tgtggagccg tcaggcgaag cttgcctatc cgattaaaga cggcattccc tatatgctgg
     2401 aaaacgaagc gcgagcgttg agcgaagagg aactcaaagc atgaccgaat tcgtcgtatt
     2461 gattccggcg cggctggatt cgtcgcgcct gcccggaaaa gccttggcgg acatccacgg
     2521 caaaccgatg gtcgtgcgcg ttgccgaaca ggcggcaaaa agtaaagccg cgcgcgtcgt
     2581 cgttgccacc gaccatcccg atattcagac ggcctgtcag gcgcacggta tcgaagtcgt
     2641 catgacttca aaccggcacg aaagcggcac gacgcgcctt gccgaagcct ctgtcgcgct
     2701 gaagctgccg ccgcatttga ttgttgtgaa cgtacagggt gacgagccgc tgattgcccc
     2761 cgaactcatc gaccgcaccg ccgaagtact cgtcgaaaac aacgtccaaa tggcgaccgc
     2821 cgcccacgaa ttgcacgatt tcgacgaatt gatgaatccc aacgccgtca aagtcgtcct
     2881 cgacaaaaac cgcaacgcca tctacttcag ccgcgccccg attccctatc cgcgtgatgc
     2941 gatacgtgcc ggaaaacgcg aaatgccgtc tgaaaccgcc gtcctgcgac atatcggcat
     3001 ctacgcttac cgcgccggct tcctgcaacg ctatgccgaa atgagcgttt cgccgctgga
     3061 aaccatcgaa tcgctggaac agctgcgcgt cctgtggcac ggttatccca ttgccgtcga
     3121 aaccgccaaa gaagcccccg ccgccggtgt ggatacgcaa gaggacttgg acagggttcg
     3181 cgccgtattt cagaccgtat aaaacaggtt caaagggaaa agatatgcag caacatattg
     3241 aaaagtggca acacttgagc cgggaagaac agaaaatcct tgctgaagta tggggtctcg
     3301 tgcaaaacga cgatcaggag gttcactatg aaatgctcaa attgaacgca cccgatgaag
     3361 ccagcggcga attttggttc agaatggcag aaacactcag caccctgccg cccaaccgtt
     3421 ccctcggcct tagaatgaac ggcggcaggc tggcgaccgc cgtatccatc ctttccgtca
     3481 tgattgaaga caatcccgac ataccgcagc tttgggcgca aaaaattacc gcgctcaatt
     3541 atagtggatt aaatt
//
LOCUS       DAWISC010000179         3551 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWISC010000179 DAWISC010000000
VERSION     DAWISC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.179"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(461..3226)
                     /locus_tag="WIW87_001840"
     CDS             complement(461..3226)
                     /locus_tag="WIW87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5174954.1"
                     /translation="MRSSFRLKPICFYLMGVTLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYKPYKNYNNQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
BASE COUNT          838 a          865 c          871 g          977 t
ORIGIN      
        1 aaaagcagaa accgctccgc cgtcattccc acgaaagtgg gaatctagaa atttaatgtt
       61 gcggcactaa ccaaaaaaac cgaaaccgaa cggtctagat tcccgcctgc gcgggaatga
      121 cgaatccatc cgtacggaaa cctgcaccac gtcattctca cgaaagtggg aatccggaaa
      181 gctaaagcca caggaattta tcggaaatgg ctgaaactta aaaaaactga ttcccgcttt
      241 tgcgggaatg acgactcata aattacccga aacaaccaaa aataaccgaa atcaaaaacc
      301 ggcttccctg cgtgggaatg aaatggattt ttctttgagt tgccgatgcg ataaaacgga
      361 ttggcatagg cttgcggcag gatgatgcgt ttggggaaac aaatgccgtc tgaagggctt
      421 tcagacggca ttgcggtttt ctacaaaatg cgggctgcct ttaaaacttg tagctcatcg
      481 tcatcaaaaa ggtgcgtccg cgtgcaaaat tggtcaatac gcttttgctt gtgccgccgt
      541 atttgccgtt gcacaacgtt ttatcagcat tacacgttac gtcttcgtcc ttgtctttcg
      601 ggtcgaacga gctgtaataa cgctgcgttg ccgcatcatt gcccgcatcg agcggatcga
      661 tataacgcct gtcgaacaga tttttgactt cggcgcggaa aataaggttt ttcttcggct
      721 cgtaagcggc gtaaaaatca aaaatcaaag gctggcgggc aagagtttcg gtttgtttga
      781 tggaacgctt gcccagttgc cggaaattgc tggtatttcc cccgttggtg ccgtcgatat
      841 agcgttcttc agccgtcgcg cggatgctct tgccgaaata gcgcatcgcg ccgcccaaag
      901 tcagtttgtt gcccaaccag cgcgtaccga cttccaaacg tccgtaatct cgcggcaggg
      961 cggaaaccct gctcaaccca taaccttgtt tgagttggtc ttctttggac gcattgttgg
     1021 gcgattcgct cgcatcgctg aagttggtcg gttgcgtgct tttttgatag gcgtaagaaa
     1081 ggttggtgaa aaaacgccca taatcgtaat tcagctccaa ctcaaaaccg tgtttgtgca
     1141 ctttgtcttt gaaattgcga tgttggatgg tgtaggcaag cccggtgctg ctgacccagc
     1201 tcggaatatc cccgttcaaa tcccaccatt tcccgtaaac gttgtggatg tagttgtcga
     1261 tgcggctgcg gtagccgacc agttttaatc ctaatgtatc atcttgtttt aacaatcctt
     1321 ttttataggt attgaagcca aattgccaag tgtttgcgcg ctctggtttt aaggcggtgt
     1381 gaacgccgga gtcgccgatt tgggaaaaat acatttcttg gatgttgggc atacggtgtg
     1441 tgcgcgaata gctggcgaac ggcatgaaat aatcgccgaa gtccgcacta atgctgaccg
     1501 aatggttgtt ggcgcgcttt ttgccgtatt ttttcaatac gggttcataa atcccgcagc
     1561 tccggttgca atgtttcttg tatgtcggcg agttttctcc gaatgcccgc ttaaattcgt
     1621 catccgagcc gtaatagccc gtatattcgc cgccgaaacg gtagccgacg gtattggtgc
     1681 tgtagtttaa gcggtaaatg tcttttttga gcgcggcatc gaagtagaac gtgttgaaat
     1741 attggctgcc ggccggttgg acaatggttg atttttgggg cagcagccct ttatcgccct
     1801 taaaccgccc caaataggaa taaagcccgt tgtcctgatc aggaccgtcg aaaaacagcc
     1861 ccaattcttc aggaaagcgg tttttgccgt attcgttgtg gaaataattg aagcccaaag
     1921 tggtttgcaa ctcggtttcg cggggcagcc ggaaggtggc ggtgttgttg aggtcgagga
     1981 ttttcgcgtt gttgtaggtt tcaaaatcct ttaaaagccc ccagcctgta aacttcgacc
     2041 ctttcggata tttctgcctg cccgaattgt aggctgcggt cagattgagg ttggtatacg
     2101 ggttcaaaga caaaccgtaa ttgaactgat aattgcggtt gatgattttg cggctgccga
     2161 ttttggtgtt taaatcgcga aattgcgccg tgtatttatt gaatacgccg tcgtattcca
     2221 atttaaacag attgcctgcc gactgctgct tcaggctgga cggatcgatg ggggtaatgt
     2281 cgtattgcgg tgccaggttt tcccgccagc ttttgtcatg ctcttcgatg tatttttgta
     2341 gttcttggtt gttgtaattt ttatacggct tgtatttcca ctgttgcctt tgtaaatccc
     2401 gctcccattt tccgctgtcg gaattgaatt tcaaagcacc ctcttgtaca aaatatcgct
     2461 gcttgcgccg ttccaaatat tccgcgccaa aatttccgat gtgctgcccg ccgccgccca
     2521 cgcggtaatt ttgcgccacg ctgcgcctgc tgtgcccgta aagcacaccg acagatgctc
     2581 cgctttccag ccatttgcgc gcacctatcg ccgccatcgc attacctttg gttgaattgg
     2641 tgccggtcag accttttagc agcaggccgt aggtattatt gccctgaacg acgtcatcca
     2701 cgcctaaagt ccgcagattc gccgaaccgg caaggctgtt gatgcctgcc gagccgctga
     2761 agctgccttt gacgacatcc agtccggcaa taaaattgct gtcgacagat gcaccgaatt
     2821 gagatgaacc gcctgccctg cccgcatcgg tagaagtcga ataaaaggtc tgcgtgatgc
     2881 cgtccaccat cgtattgacc cgcccgaacc cgctgtcgcc gcgaatattc aaagacacaa
     2941 tgcccgagct tttatcttgc tgtgtaaacg caccggggat gctgcgtacg atgttgtcga
     3001 ggttttcgct ggatttgaat atatcctgac gggtcgatac ggcacgcgca tcggtaaaca
     3061 cttttttgtc tttcggtacg cgcttcgcct tgacgtgcac atcttccaaa acctgtatct
     3121 gcgcctcgct gcccgcgcgc cctgcatctt cggcataact ataatgatat agcgtaacac
     3181 ccataaggta aaaacaaatc ggcttcaacc ggaaagaaga tctcatattt tcctcaacaa
     3241 taaacagtca gacaattagg aaatatacta gcattttttt caggcggcat aaacattaaa
     3301 aaagtgtaaa tttgatatac cgtctgaaga tttcaattgg atatttatag tggattaaca
     3361 aaaaccagta cagcgttgtc tcgctttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gcaccaagtg aatcggttcc gtactgtttg tactgtctgt ggctcgccgc cttgtcctga
     3481 tttttattaa tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg
     3541 ggaatctagt c
//
LOCUS       DAWISC010000180         3547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWISC010000180 DAWISC010000000
VERSION     DAWISC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.180"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            1..1775
                     /locus_tag="WIW87_001841"
                     /pseudo
     CDS             1..1775
                     /locus_tag="WIW87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            1899..3449
                     /locus_tag="WIW87_001842"
     CDS             1899..3449
                     /locus_tag="WIW87_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ5174955.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPASLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT          995 a          875 c          905 g          772 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aggcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggattgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttgaca ggtatgtacg gcacgcaagg cacgaccagc
      241 gtgcaagtcg gctccgcaca actgattcaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgatggcg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc tgatgaaggc
      361 aaccgcaaaa tcggtttggc ccggttcggc aacaaccgcg cccaaggtac gttcgacttg
      421 ggccaacgtt tcggcgaaaa caaagcattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacaccc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtc ctgactttgg
      661 gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacgcgga acggcagccg
      721 gagctagcaa ggtagaagtg aaggcaaacg gaaatattga gcatcaattg gcatcgttta
      781 cagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat atcaaagtca
      841 acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgc ctgactttgg
      901 gcggacgttt ccaaacagtc gaatataccg acaaaaaatc acagagcggc gatgccaaac
      961 gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt gtgtacggca
     1021 actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc gaaacgacaa
     1081 tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac tggggcaatt
     1141 tcgtaaccac gttaaacgcg ttccaaatca aacgcccggg ctactggcgc ggtcatacaa
     1201 ctaccaagac aacaaaaggg gtaacaacaa cgctgaccta tggcaataat tctgatttcg
     1261 cccgctataa ggcgcaaggc ggcgcggccg gcgacgagca aggtatggaa cgcaatcgcg
     1321 gtattgagtt caacacctac gcgaacttgc tcaacaatac tttgcgtccg actttgggtc
     1381 tgatgtatct gcaatcgacc gtgaaagagt atccaaattc acgcgatatg ctggttaacg
     1441 gcgtacaagt cgccaatccg cgcgtaattg ccaaagcagg tttggaatgg gatacaccgt
     1501 ttgccaaagg cttgacttta aacggtaatg tttcgtattt cggcaagtct gaccaagaca
     1561 cgcaaaaaca atacgccttc ccgtcctata ccttgattga cgtaggcgcg cgctacaaaa
     1621 ccaagcttgg caaaaacact ctgaccgtca gcagctcggt ggaaaacctg ttcaacaaaa
     1681 actattggca ggtacagcgc ggccaatacg accgcagctt cgccgtcgtc ggtatgccgc
     1741 gcacttactg gctgaaggcg gaattggatt tctgatttcc ctatatccgg aaaatgccgt
     1801 ccgaacaggt ttcagacggc atttctttta aagagatatg gcgggatacg cttaaataat
     1861 gctattctag cgtctttgaa acctgcttgt ttgaagtgat gaaaaaacct ttgatttcgg
     1921 ttgcggcagc attgctcggc gttgctttgg gcacgcctta ttatttgggt gtcaaagccg
     1981 aagaaagctt gacgcagcag caaaaaatat tgcaggaaac gggcttcttg accgtcgaat
     2041 cgcaccaata tgagcgcggc tggtttacct ctatggaaac gacggtcatc cgtctgaaac
     2101 ccgagttgct gaataatgcc cgaaaatacc tgccggataa cctgaaaaca gtgttggaac
     2161 agccggttac gctggttaac catatcacgc acggcccttt cgccggcgga ttcggcacgc
     2221 aggcgtacat tgaaaccgag ttcaaatacg cgcctgaaac ggaaaaagtt ctggaacgct
     2281 tttttggaaa acaagtcccg gcttcccttg ccaataccgt ttattttaac ggcagcggta
     2341 aaatggaagt cagtgttccc gccttcgatt atgaagagct gtcgggcatc aggctgcact
     2401 gggaaggcct gacgggagaa acggtttatc aaaaaggttt caaaagctac cggaacggct
     2461 atgatgcccc cttgtttaaa atcaagctgg cagacaaagg cgatgccgcg tttgaaaaag
     2521 tgcatttcga ttcggaaact tcagacggca tcaatccgct tgctttgggc agcagcaatc
     2581 tgaccttgga aaaattctcc ctagaatgga aagagggtgt cgattacaac gtcaagttaa
     2641 acgaactggt caatcttgtt accgatttgc agattggcgc gtttatcaat cccaacggca
     2701 gcatcgcacc ttccaaaatc gaagtcggca aactggcttt ttcaaccaag accggggaat
     2761 caggcgcgtt tatcaacagt gaagggcagt tccgtttcga tacactggtg tacggcgatg
     2821 aaaaatacgg cccgctggac atccatatcg ctgccgaaca cctcgatgct tctgccttaa
     2881 ccgtattgaa acgcaagttt gcacaaattt ccgccaaaaa aatgaccgag gaacaaatcc
     2941 gcaatgattt gattgccgcc gtcaaaggag aggcttccgg actgttcacc aacaatcccg
     3001 tattggacat taaaactttc cgattcacgc tgccatcggg aaaaatcgat gtgggcggaa
     3061 aaatcatgtt taaagacatg aagaaggaag atttgaatca attgggtttg atgctgaaga
     3121 aaaccgaagc cgacatcaga atgagtattc cccaaaaaat gctggaagac ttggcggtca
     3181 gtcaagcagg caatattttc agcgtcaatg ccgaagatga ggcggaaggc agggcaagtc
     3241 ttgacgacat caacgagacc ttgcgcctga tggtggacag tacggttcag agtatggcaa
     3301 gggaaaaata tctgactttg aacggcgacc agattgatac tgccatttct ctgaaaaaca
     3361 atcagttgaa attgaacggt aaaacgttgc aaaacgaacc ggagccggat tttgatgaag
     3421 gcggtatggt ttcagagccg cagcagtaat gggtcaaatg ccgtctgaaa tgttcaggcg
     3481 gcattttgtt gtattggaaa ctgccgtttt caaccattcc tttagataag ccgtgtcgga
     3541 ttcgttt
//
LOCUS       DAWISC010000181         3544 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWISC010000181 DAWISC010000000
VERSION     DAWISC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.181"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="WIW87_001843"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="WIW87_001843"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ5174956.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKNFKIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            complement(1380..1838)
                     /locus_tag="WIW87_001844"
     CDS             complement(1380..1838)
                     /locus_tag="WIW87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ5174957.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1957..2778
                     /locus_tag="WIW87_001845"
     CDS             1957..2778
                     /locus_tag="WIW87_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5174958.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WIW87_001846"
     CDS             complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WIW87_001846"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ5174959.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          835 a          767 c         1023 g          919 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgtttgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaga aacttttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgttatacc atattgcaga tgaaggtggc ggaaaggatt
      301 atggcgcagc atccgggcga gcggttttat gtggtgctga tgtctgaaaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ctttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggta
      481 aagtcgatgc tgctgccgaa agtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 ggcaatttaa ttcaaagcag cagctatttg ggcgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgcaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga agacggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaagaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaaaat acaatatgtc gcgccgcatc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgagatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggcgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaaa attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tctgattgct ttgcgtgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagt cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaaact gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcgtccag tttgccggtt tgggcggcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagt
     1561 gcgcggccgt tgccttgcgg atcggactca aaaagtgtga acggtttctt gctgctttcg
     1621 gcaaacgacg ctgccgcctg tttgaatttt tcgacatcgt aaggttcttc gtctttgacc
     1681 attttgccca tgcgtgtgaa ttcgggcatc atggatttga acgcggcggt gcggttttcg
     1741 gaaatttcgc ctttgggttg ggaaggtatt ccgctgcctc cgcaggcgga aaggagcagt
     1801 gtgatggcgg cagcagcaag gctgatttgg gttttcatat tgaatgtgtc ctgtcgtggt
     1861 ggtatggttg ttgtcatttt cagtcggcgc gatgccgtct gaagggtgtt atgatacctg
     1921 aaacaggttt gggaaacgag aaagaaagga aaaacaatgg ctgttttaat taccggtgct
     1981 tcggcaggat tcggcgaagc gatgtgccgt gcgtttgtcg gggcgggata ccgcgttatc
     2041 ggtgcggcgc gccgtgcgga caggcttcag gccttggcgg atgaattggg tgctttgttt
     2101 taccctttgg aaatggacgt gtcgcgacgc gagtcggtgg aaaacgcctt aaacggcatc
     2161 cccgatgaat tttccgacat cgactgcctc atcaacaatg ccgggctggc tttgggtctg
     2221 gacacggcgg acaaggcgga ttttgaagat tgggaaacga tgattcaaac caatgttttg
     2281 ggtttgacgt tcctgacgcg caaaattttg ccgcaaatgg tggaacgcgg cggcggttat
     2341 gtgatgaatt tgggttcgat tgcaggcaat tatgcttatg ccggcagcaa cgtttacggg
     2401 gcgaccaagg cgtttgtgcg ccagttcagc ctgaatttgc gcgcggagtt ggcggataag
     2461 aacatccgcg ttaccaatat cgagccgggt ttgtgcggca atacggagtt ttccaatgtg
     2521 cgcttcaaag gcgatgacga gagggcggcg ggcgtgtatg agggtgtgga atttatccgc
     2581 cccgaagata ttgcggaaac cgcattgtgg ctgtaccagc ggccggcgca tatgaatgtg
     2641 aacacgattg aaattatgcc cgtggcgcag acttttgcag gaatgaaagt gataaaaaaa
     2701 gccgtgcccg aagtgcggga agactttgaa aaacagagta tgtcgctgtt ttcccgcatc
     2761 aggtcctggt tcaaatgatg caaaatgccg tctgaagaca gtttcagacg gcatttttac
     2821 gggtattttt acggagtagg caataagccc gccaatttgg ggttgccttc tttcggaatc
     2881 gggcgcggat tgccttccgc atcgatggca acataagtga acacggcttc ggttacgagg
     2941 tagcggtctt cggtaacgca atcgttcatc aaagttttca cccagacgtc gactttaagc
     3001 tggagggaag tgttgcccac gcggacgcaa tgcccgtagc agcagacgac gttgccgacc
     3061 ttgaccgggc ggatgaagtt catttcctga acggcgacgg taacgatgcg tccccgcgcg
     3121 atttccgccg ccaatatgcc gccgcccaaa tccatctgcg acataatcca gccgccgaaa
     3181 atgtcttggt tgggattggt atcgcgcggc atagcgacgg tacgcaggag cagttcgcct
     3241 tgagggcgtt ggcggttgcc ttcttcgtgc tgcataaagt ttccttgttt tattgaaata
     3301 taaatcgaac ctgcacccct gcccgaaacg ggaagtcggg aagtgtgtag tttaacccat
     3361 ttgagacata caagggcggg cgcggaacag attcgcaagg cgtattgtag ggcggggctg
     3421 tagagtgggc ttcagtccgc caatcccgcc aaatcctacc ctaagcaact gaaccgtcat
     3481 tcccacgaaa gtgggaatct agaacgcggg gtttcagtca tttccgatag attcccgccg
     3541 cgtc
//
LOCUS       DAWISC010000182         3466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWISC010000182 DAWISC010000000
VERSION     DAWISC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.182"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            185..1060
                     /locus_tag="WIW87_001847"
     CDS             185..1060
                     /locus_tag="WIW87_001847"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ5174960.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            1069..2265
                     /gene="bamC"
                     /locus_tag="WIW87_001848"
     CDS             1069..2265
                     /gene="bamC"
                     /locus_tag="WIW87_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ5174961.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WIW87_001849"
     CDS             complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WIW87_001849"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ5174962.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          926 a         1146 c          767 g          627 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc ggaagcatta ccgcccctac accacaactg ataaaaggtt
      181 ctgtatgtta caaggtagct tggttgccct gattaccccg atgaatcaag acggcagcat
      241 ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca cggacggcat
      301 cgttgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac acaccgccgt
      361 catcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc
      421 aaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg gggcggacta
      481 caccctctcc gtcgtcccct attacaacaa gccctcccaa gaaggcattt accaacattt
      541 caaaaccatc gccgaagcca cttcgattcc gatgattatc tacaacgtgc ccggccgtac
      601 cgtcgtcagc atgactaacg acactatcct gcgccttgcc gaaatcccca acatcgtcgg
      661 cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc gcgcgcccga
      721 aggcttcgtc gtcctttccg gcgacgacca caccgccctg ccgttcatgc tctgcggcgg
      781 acacggcgtg attaccgtag ccgccaacgc cgcgcccaag ctctttgccg atatgtgccg
      841 cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccgac tgattccgat
      901 ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg tatccgccct
      961 gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaaa acggacaagc
     1021 aaaagtccgc gccgccctga aagcctcagg acaactttaa atatagccat gccgtccgaa
     1081 ccgttcggac ggcataacgc aacaaacact ttaatatcca tcacacagga tgacacgatg
     1141 acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc
     1201 agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct gatcaaactt
     1261 gaagtcccac ctgatttgaa caaccccgac caaggcaacc tctaccgcct gcctgccggt
     1321 tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt ccaacagcct
     1381 gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa
     1441 cgctggctcg ttgtcgacgg caagtctcct gccgaaatct ggccgctcct gaaagccttt
     1501 tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca aatggaaacc
     1561 gagtgggcgg aaaaccgcgc caaaatcccc caagacagct tgcgccgcct cttcgacaaa
     1621 gtcggcttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa
     1681 cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa agaagtgtac
     1741 ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc caacctcgaa
     1801 gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca ggcggaaaac
     1861 gcatcggcaa aaaaacctac ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa
     1921 agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgtgct cgccctcgac
     1981 cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgccttcct ggttcaaaaa
     2041 gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa acgcctgctg
     2101 ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta tgcagaacct
     2161 gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa
     2221 gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccat tcaaaacaaa
     2281 agcagcttcc aaacagaagc tgctttttta tgcggaactt tttacaacaa atggggcaaa
     2341 atatcctcaa ctgcctgaac caacgcatcc accaaaggcg gatcgaacgc acgatgccct
     2401 gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca cgcactctgc
     2461 atcggcgtac acaaatcata ccgcccctgt acgataatag tcgggatatg ccgtattttg
     2521 ccgatattgt tcaaaatcgc cctatcgccc tgcaaccaac cgccgttgac aaaataatgg
     2581 ttttccaaac gcgcgattgc cagcgaggca taagcatctt catccacttc ctccggctcg
     2641 aaacggatca gatagctttc ccaatccgcc caagccttcg cggcagacag gcacacttct
     2701 tcatcttgat gaaacagcaa tccgtgatac gcctcaatca gccggttccg ccgattttca
     2761 gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac accgcccgcc
     2821 tcgttcagcc acaccgtttc agacggcctg cacaaaaata tcccgcgcaa caccaatccc
     2881 tttacccgtt caggatgggt ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg
     2941 ccgaacacca gccatttccc gatacccagc atttcacgga ctttttcaat atccgccacc
     3001 aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc
     3061 tggtcgatga tgacgatgcg gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa
     3121 gcccccgcgc ccggcccgcc atgtaaaaaa ataaccggca caccgtcggg attgcccgat
     3181 tcctcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg aaaaggctgt
     3241 tttatctcat acatcatctt ctcccatatt gcttctgaca atccgaacat tcccacacgc
     3301 aacatcacgc acgagacaca agccgccccg aaaggcaaat gccgaaaaat cgcgatatag
     3361 tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacggata gtacggaacc
     3421 gattcactcg tgcttaagca ccttagagaa tcattctctt taagct
//
LOCUS       DAWISC010000183         3434 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWISC010000183 DAWISC010000000
VERSION     DAWISC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.183"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="WIW87_001850"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="WIW87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ5174963.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="WIW87_001851"
     CDS             1455..2555
                     /locus_tag="WIW87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ5174964.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAIHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAARLAVALFDGKDAPERDKESGLAYIRRQD"
     gene            2577..3194
                     /gene="thiE"
                     /locus_tag="WIW87_001852"
     CDS             2577..3194
                     /gene="thiE"
                     /locus_tag="WIW87_001852"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ5174965.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          695 a         1080 c          950 g          709 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgccccat tttcatgttt tttaaggact tgatatgtcg
      241 ggcaatgcct cctctccttc atcttcctcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtatcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcagcgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga gctgtccgcc gtgatgccgc tttcctggct gccgcttgcc
      841 gccgactaca cgcgccacgc gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac cacaacgttt ctcgatgcct attccgccgg cgcgagtgcg
     1081 aacaacattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtacttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggcttgcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcgttgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag
     1801 cggatgacga aatcgtccgt tggcgcgccg acgacatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccctgccat tgggaacacg
     1981 aatgcgtccc cgaaggcctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaac caatcccccg agcacaccag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccatccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgccag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgcgataaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg cccgaaagga caccttatga
     2581 ccttcccgcc cctaaaatcc ccgctcaaat tctacgccgt cgtccccacc gccgattggg
     2641 tggggcgcat ggtcaaagca ggtgccgaca cggtgcaact gcgctgcaag gccctgcacg
     2701 gcgatgaatt gaaacgcgaa atcgcccgct gcgccgcagc ctgtcagggc agccgtacgc
     2761 agcttttcat caacgaccac tggcgcgaag caatcgaagc gggcgcgtac ggcgtgcatc
     2821 tcggacaaga agacatggac accgccgacc ttgccgccat cgccgccgcc ggtttgcgct
     2881 tgggtttgag tacgcactcc gttgccgaac tcgaccgcgc cctgtccgta caccctagct
     2941 acatcgccag cggcgcgatt ttcccgacca cgaccaaaca aatgcccacc gccccgcaag
     3001 gcttggacaa actgcgcgaa tacgtcaaac aagcaggcgg cacgcccgtc gtcgccatcg
     3061 gcggtatcga cttgaacaac gcccgagccg tactcgccac cggcgtttcc tcactcgccg
     3121 ccgtccgcgc cgtaaccgaa gcggcaaatc ccgaagcggt ggttaaagcg tttcaggctt
     3181 tgtgggatgg ataaaaccga aagaagaaaa ttcaattgcc gtgtaggcaa aacttagccc
     3241 gttatcgcaa acatacttaa ctttaaatgt ggcatatcat caaattccgt cattcccgcg
     3301 taagcgggaa tccgccttaa aacttgagaa accatcattt gaaaaacagt ttccgaattt
     3361 caaaaatgga ttcccgcccg tgcgggaatg acggcaaccg gtcagttgcg tatcaaaaaa
     3421 taaagtaatt cggc
//
LOCUS       DAWISC010000184         3426 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWISC010000184 DAWISC010000000
VERSION     DAWISC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.184"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            142..1285
                     /locus_tag="WIW87_001853"
                     /pseudo
     CDS             142..1285
                     /locus_tag="WIW87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1395..1604)
                     /locus_tag="WIW87_001854"
     CDS             complement(1395..1604)
                     /locus_tag="WIW87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174966.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="WIW87_001855"
     CDS             complement(1601..1897)
                     /locus_tag="WIW87_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ5174967.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="WIW87_001856"
     CDS             complement(1876..2481)
                     /locus_tag="WIW87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174968.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="WIW87_001857"
     CDS             2561..2848
                     /locus_tag="WIW87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ5174969.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="WIW87_001858"
     CDS             2820..3122
                     /locus_tag="WIW87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ5174970.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
BASE COUNT          734 a          916 c          914 g          862 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcgacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacgg ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcgggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac
     3301 ccctgccgcc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agccgtttcc
     3361 gatagattcc tgccgcgttt ggggtctgga ttcccgcctg cgcgggaatg acggtggcgg
     3421 ggggtc
//
LOCUS       DAWISC010000185         3418 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWISC010000185 DAWISC010000000
VERSION     DAWISC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.185"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            43..711
                     /locus_tag="WIW87_001859"
     CDS             43..711
                     /locus_tag="WIW87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ5174971.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTEDVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPRGDRRTM
                     CQYK"
     gene            735..1355
                     /gene="pilV"
                     /locus_tag="WIW87_001860"
     CDS             735..1355
                     /gene="pilV"
                     /locus_tag="WIW87_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ5174972.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLPHTLSAVDGDFA
                     IDAMKTKGQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTLSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTDLEVSGDNIVYTYQARVGGRE"
     gene            1352..2293
                     /locus_tag="WIW87_001861"
     CDS             1352..2293
                     /locus_tag="WIW87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ5174973.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVIPDTTQQNSP
                     FSLKRNGIDKLIPIAESSNINYQNFFQVGSALIFQYGIDDVNASTATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHVVNAYAVGRIADEEGLFRFQLDDKG
                     KWGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSAQNAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2272..2865
                     /locus_tag="WIW87_001862"
     CDS             2272..2865
                     /locus_tag="WIW87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ5174974.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCAAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMEYKKGKRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2870..3343
                     /locus_tag="WIW87_001863"
     CDS             2870..3343
                     /locus_tag="WIW87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ5174975.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIENKLEIFVSGYKMNPKIAKKYSVSVKFVDKEKSRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK"
BASE COUNT          980 a          680 c          865 g          893 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacgccca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggagg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtcagactc agccgaccac cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaaaa
      541 caatccagct ttttttattc tgacggttat atccaaatcg tgttgacaga tgcgaaggcg
      601 gtttctgccg atgaaaagaa attccgttcg gcggtggttt tgattaacag cagcggcagg
      661 gtcgaagttt gccctagggg tgatcggcgt actatgtgcc agtataaata acagtttcag
      721 ttttaaaaat gaatatgaag aataatgatt gcttccgcct gaaagattcc cagtccggta
      781 tggcgctgat agaagtcttg gttgctatgc tcgttctgac catcggtatt ttggcactat
      841 tgtctgtaca gttgcggaca gtcgcttccg tcagggaggc ggagacacaa accatcgtca
      901 gccaaatcac gcaaaacctg atggagggaa tgttgatgaa tccgaccatt gattcggaca
      961 gcaacaagaa aaactataat ctttacatgg gaaaccatac actaccgcat acactatcag
     1021 ctgtggatgg cgattttgcg attgatgcca tgaaaactaa ggggcaattg gcagaggcac
     1081 aattgaagag atttagttat gagctgaaaa atgccttgcc ggatgcggca gccatccatt
     1141 acgccgtctg caaggattcg tcgggtaacg cgccgacatt gtccggcaat gctttttctt
     1201 caaattgcga caataaggca aacggggata ctttaattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatt tcccgtacgg atcttgaggt gagcggcgac aatatcgtat
     1321 atacttatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga aaggttttac cattattgaa tttttggttg cgggcctgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acatcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ccgcgcaaca ggatttgcgg aatgcggcaa cattgattgt
     1561 ccgcgatgcg agaatggcag gcggcttcgg ttgtttcaat atgtccgagc atcctgcaac
     1621 tgatgttatt cccgatacga cgcaacaaaa ttctcctttt tccttaaaaa ggaacggtat
     1681 agataaactt attcccatag cggaatcttc aaatatcaat tatcagaatt ttttccaggt
     1741 tggtagcgca ttgatttttc aatacggaat cgatgatgtt aatgcaagca ccgcgactac
     1801 cgtcgtcagc agctgtgccg caatatcgaa accgggcaag caaatcccta ctttagaaga
     1861 tgcaaaaaaa gaattgaaga ttccggatca ggataaggag caaaatggca atatagcgcg
     1921 tcaaaggcat gtggtcaatg cctatgcggt cggcaggatt gccgatgagg aaggtttgtt
     1981 ccgcttccaa ttggatgata agggcaagtg gggtaatcct cagttgctcg tgaaaaaggt
     2041 tagacatatg aaagtgcggt atatctatgt ttccggctgt cctgaagatg acgatgccgg
     2101 caaagaggaa acattcaaat atacggataa attcgacagc gcccaaaatg ctgttacgcc
     2161 cgccggggtg gaggttttat tgagtagcgg tactgatacc aagattgccg cttcttcaga
     2221 caatcatatt tatgcttacc gtatcgatgc gacaatacgc gggggaaatg tatgcgcaaa
     2281 cagaacactt tgacgggaat cccgacttct gacggacaga gggggtttgc actgtttatc
     2341 gtgctgatgg tgatgatcgt cgtggctttt ttggttgtaa ctgccgcgca gtcttacaat
     2401 accgagcagc ggatcagtgc caacgaatca gacaggaaat tggctttgtc tttggccgag
     2461 gcggctttgc gggaaggcga acttcaggtt ttggatttgg aatatgatac ggacagtaag
     2521 gttacattta gcgaaaactg tggaaaaggt ctgtgtgccg cagtgaatgt gcggacaaat
     2581 aatgataatg aagaggcttt tgacaatatc gtggtgcaag gcaagcccac cgttgaggcg
     2641 gtgaagcgtt cttgccctgc aaattctacc gacctgtgca ttgacaagaa agggatggaa
     2701 tataagaaag gcaagagaag cgtcagcaaa atgccacgtt atattatcga atatttgggc
     2761 gtgaagaacg gagaaaatgt ttatcgggtt actgccaagg cttggggtaa gaatgccaat
     2821 accgtggtcg tccttcaatc ttatgtaagc aataatgatg agtaataaaa tggaacaaaa
     2881 agggtttaca ttgattgaga tgatgatagt cgtcgcgata ctcggcatta tcagcgtcat
     2941 tgccatacct tcttatcaaa gttatattga aaaaggctat cagtcccagc tttatacgga
     3001 gatggtcggt atcaacaata tttccaaaca gtttattttg aaaaatcccc tggacgataa
     3061 tcagaccatc gagaacaaac tggaaatatt tgtctcaggc tataagatga atccgaaaat
     3121 tgccaaaaaa tatagtgttt cggtaaagtt tgtcgataag gaaaaatcaa gggcatacag
     3181 gttggtcggc gttccgaagg cggggacggg ttatactttg tcggtatgga tgaacagcgt
     3241 gggcgacgga tacaaatgcc gtgatgccgc ttctgcccaa gcccatttgg agaccttgtc
     3301 ctcagatgtc ggctgtgaag ccttctctaa tcgtaaaaaa taaggttgtt ttgccaatac
     3361 cgtctgaaaa tcaatgttca gacggtattt ttatgggtat agtggattaa caaaaatc
//
LOCUS       DAWISC010000186         3365 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWISC010000186 DAWISC010000000
VERSION     DAWISC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.186"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..158
                     /locus_tag="WIW87_001864"
     CDS             <1..158
                     /locus_tag="WIW87_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174976.1"
                     /translation="LKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTV
                     YLPGLHP"
     gene            170..679
                     /locus_tag="WIW87_001865"
     CDS             170..679
                     /locus_tag="WIW87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174977.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATATAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(732..1079)
                     /locus_tag="WIW87_001866"
     CDS             complement(732..1079)
                     /locus_tag="WIW87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5174978.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1081..1317)
                     /locus_tag="WIW87_001867"
     CDS             complement(1081..1317)
                     /locus_tag="WIW87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ5174979.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDAGNEVV"
     gene            complement(1676..2167)
                     /locus_tag="WIW87_001868"
     CDS             complement(1676..2167)
                     /locus_tag="WIW87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ5174980.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2605..2973)
                     /locus_tag="WIW87_001869"
     CDS             complement(2605..2973)
                     /locus_tag="WIW87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ5174981.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          849 a          817 c          781 g          918 t
ORIGIN      
        1 cgctgaaatt cgaccagttg atactcgaat ttcctgagcg cggggacggc gcatgggtac
       61 acatcggttt ccgacgcaac agcccgcaac gcaaccagat actgaccgca accaagaaaa
      121 acggcaaaac cgtgtatctg cccgggctgc atccttgagg tcgtctgaaa tggatatttt
      181 attgaaatac tggaagccgg taggtgtatt gctgctaatc gtcctgattt ttaccgcatg
      241 gcatttcgac cgtgccgaaa aataccgcat gggacgggag gctgctgctg ccgaaatctc
      301 gaatcgtctg aaagacggct atatcgagca ggcaaagcag gcgcgttctg ccgagcagaa
      361 ggccgctgcc gcgtttgccg aacgacaaac caaattagaa gaggaaaaac aaaatgctga
      421 aaaaactgtt gccgctatgc gtcttgagct taaccgcctg cgccactacg ccgccgccca
      481 aaatcgcaac agaaacctgc ccgcaaccgc taccgccgcc accgcatctg atggcgcgtc
      541 agattcccaa ggctggctat tattcggaca gtgcgctgaa aaatatgcag gactggcaga
      601 aacagccgac ggacatgcag ccgatttaag ggaatggcag gcatatggtc atgcagtaag
      661 cagtcagcgt gccgaataag caaccgcccg aacctgtaag aaaagattac aggttcgggc
      721 ggtttcagca tttaatcgaa taagacggcg ccgatgcgcg ccagcacatc gtccaataca
      781 taatcgggta cagtttcttt aaaagaagcc ttgcggattt gccagtctag agatttgagc
      841 tggtggcagt ggacattgcc ctgcgtttcc gttcctgcac cgagtaaggt tgaaatcatg
      901 ccgctgcttc gtgcagctgc tgcattcccc tgtgaaatgg ggcaggcaaa aaccaatccc
      961 gttgcgcggt tgaatgcttt tggagacaga gccagcgcaa accgcccgcc cttgatttcc
     1021 ttgccgctgg aagggtcgaa attcaaatgg aaaatatcgc ctttgtcggg aatatacatt
     1081 tcagacgacc tcgttgccgg catcatccaa gatttcccag ccttctacgc gcggcggggt
     1141 ttcttccatt tcggcaagca agtctgccaa gcggaaacgt cgggcagcac gcacacggag
     1201 ttcgccgtta tgtacttccg ctaccaaagc gtcgccgatt ttaaaatcca attgtttcag
     1261 catgtcggca ggcagtcgga cggccgccga gttcccccat ttttggacac gcaacataat
     1321 cttcaccttt attgtatcta caaagtagat acatattacc ataaaatttc agttgttcaa
     1381 atacttgtgc agaatacgcc aaaagccgtc cgaactgttt cggacggctt ttgtactgta
     1441 tttgcgcctt caggcaatat tttgttatcc attttcaaga tgcaaaagct ttctaattgc
     1501 ttgatgtcgg atttcggttg tttagggata caaaaccaag taaactaaaa ctgtttgatt
     1561 ggaaaatgct ccgcaaggag aaaattatgt tcaaaaaatc actttataag gctgctttgg
     1621 cgtatttcgg cgattgcgtg gctgcccata tttcagaaca gttttgactg tttattcaca
     1681 aatcagatgc ctttaggggg tttgctttcc ataatgcaac caaaatttcc aactctctaa
     1741 atattgtgtc tttgcgttct tcttcgcgta ttttcataac aagaggcgaa actgcattcc
     1801 acaattttat gaagttggtg caatgcagcc gtttaaacaa atcctcatcc aatactcccg
     1861 agttgattgc gcacgcataa aactcgtgcc gattgattac ggtaagtaaa tgccctctct
     1921 ctttttccca ttgtccgccc ttattttgat aaatttcata caggtcgatc cgcgcgttgt
     1981 tgtcgtcaac tgccgtgccg cgaatataag gcgaaatatg attgtcggct tctacaaatt
     2041 gtgcatcttg gtaatcattc aaaataacat ctaatgtcgc cttttgcttt gaagttttct
     2101 tattgatgaa tattgtcccc agtgcaatga cggcggttac tgaaacaacg gtcagttgcc
     2161 aaaacatcag ccattcggcc gtccctcata ggttaaactg tacgctgtgc ctgaatatgt
     2221 aaaataaagt gaatacagat acaaacaagg aggttagggc aattactgac aaccaacctc
     2281 cttgttttat ttgtttccgc accttaccag ccattaaaac cctcattttt gtatttcatc
     2341 gtatttcctt aatggtttta accttgcttg tcgatatctg tatattaatg ccgtgcgccc
     2401 gttctttcaa ggggtgattt aaaaatcagg catccttgac atcctctcct gcttaaaggc
     2461 ggggggactc ctgccgtgta aaccaatgcc gtctgaaggg ctttcagacg gcataaaaaa
     2521 accgcctttg tgtaaagcgg ttgaagaaaa gcctttcaat aaaaatgccg tctgaatttc
     2581 agacggcatt gttgtcggat atgcctattc cttatccagc cggcgcaggg tttcggcgag
     2641 ctgtttgaag tcggtttctc ccgccgccaa actgacaatc aagtcgtcaa gcccttggtc
     2701 ggcggcaatg ctgatgccct gcaaatccag ataggtcaac attgttaaaa gcgcggtgcg
     2761 cttgttgccg tcgggaaagg cgtgggcttt ggctatggct tgtgcataga gggcggcgat
     2821 ttcgtagatg tcctcaaggt tttcatactg ccgccagttg gcaatccgcg acaatgcgcc
     2881 gtccaagcgc gccatatccg cccgcccttt caaacccgct tcatccgcca atacggtttg
     2941 atggataagc gcgaccagtt cgccgtctat catttgtcgg caagtgcctt gacggctttt
     3001 tgatgggttt tggcaatgcg gcgggcggcg gcaagcagta cgcgtttgcc tgcttcgcct
     3061 ttaagctcga ttttgacggg gcgtgtattt tggttttgca tcgctgctcc ttgtctgttt
     3121 gcgggcattt tagctttttt ccggcagctt gggaaatgcc gtccgaaaac acttcagacg
     3181 gcattcttct aatagtgtaa tgcaatagtt actccaagat ttttgtatat ggctgttttt
     3241 atggtaaatt aaattttcat aagaaataaa tatatttaaa tcaatgaaat aaaatttagt
     3301 cgaatcccac cgtttccgcc acaaagcaaa accgccctga ttcggggcgg ttcttttttg
     3361 ttcaa
//
LOCUS       DAWISC010000187         3322 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWISC010000187 DAWISC010000000
VERSION     DAWISC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.187"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..80
                     /locus_tag="WIW87_001870"
     CDS             <1..80
                     /locus_tag="WIW87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174982.1"
                     /translation="LRYMLLALAWAVAAFFCIRTVSREV"
     gene            536..820
                     /locus_tag="WIW87_001871"
     CDS             536..820
                     /locus_tag="WIW87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ5174983.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            821..1924
                     /locus_tag="WIW87_001872"
     CDS             821..1924
                     /locus_tag="WIW87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ5174984.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCACYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKP"
     gene            2223..3179
                     /locus_tag="WIW87_001873"
     CDS             2223..3179
                     /locus_tag="WIW87_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ5174985.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLNPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1101 a          706 c          772 g          743 t
ORIGIN      
        1 ggctaaggta catgcttctc gcccttgctt gggcggttgc cgcctttttt tgtatccgca
       61 cagtatctcg tgaagtctag caggcgcagc accgccgggc ttcagtaact tgtaccaagg
      121 cagggggagg acgtccagaa agatttgtaa agacggcttt atcgtcttta taaatctttt
      181 tggatacccc ttgccgcccc gccaaaagaa cacattctgc cgcaagggca ggtggtaagg
      241 cgcgcgcctt ttgcgccgtc cccatgcccc cgcggcgtcg caagtgagac tagggggtgt
      301 gggggactag tcccccgcaa agcgttcagc ttcggaaact ttggccgaaa ggcaggcgaa
      361 gcagcgcact ttgcgacgaa tgtcgcaaat agccgagaag cgcgggggga ttggcgataa
      421 gcgcgagggg ggtgtcccca cagcgccgcc gcgccgcgaa tgcggcgcaa aatctttcag
      481 attaagaaac atttgtttaa tgaggcaacc gtgcctttta agaaagggat agcaaatgaa
      541 attgttggcc gcattgattc cgcttttgat gagcgtggca ggccgtatat tgactgcatt
      601 aggcttgatg gcggtaacct attcaggggt ggatagattg gtagcccatt ttcagcaggc
      661 gataaccaat agcataacgg gcgcgcctca agcgatgttg cagctttttt atataagcgg
      721 cggtggaacc gttcttaata tcctgtttgg cgcgatcgcc tttattctgt cattcaaaca
      781 aatgacaaaa ctagcaacct caatcgggaa gaaaaaataa atggcagaga tctgtttgat
      841 aaccggcacg cccggttcag ggaaaacatt aaaaatggtt tccatgatgg cgaatgatga
      901 aatgtttaag cctgatgaaa acggcatacg ccgtaaagta tttacgaaca taaaaggctt
      961 gaaaataccg cacacctaca tagaaacgga cgcaaaaaag ctgccgaaat cgacagatga
     1021 gcagctttcg gcgcatgata tgtacgaatg gataaagaag cccgaaaata tcgggtctat
     1081 tgtcattgta gatgaagctc aagacgtatg gccggcacgc tcggcaggtt caaaaatccc
     1141 tgaaaatgtc caatggctga atacgcacag acatcagggc attgatatat ttgttttgac
     1201 tcaaggtcct aagcttctag atcaaaatct tagaacgctt gtacggaaac attaccacat
     1261 cgcttcaaac aagatgggta tgcgtacgct tttagaatgg aaaatatgcg cggacgatcc
     1321 cgtaaaaatg gcatcaagcg cattctccag tatctataca ctggataaaa aagtttatga
     1381 cttgtacgaa tcagcggaag ttcataccgt aaataaggtc aagcggtcaa agtggtttta
     1441 cactctgcca gtaatagtat tgctgattcc cgtgtttgtc ggcctgtcct ataaaatgtt
     1501 gagcagttac ggaaaaaaac aggaagaacc cgcagcacaa gaatcggcgg caacagaaca
     1561 gcaggcagta cttccggata aaacagaagg cgagccggta aataacggca accttaccgc
     1621 agatatgttt gttccgacat tgtccgaaaa acccgaaagc aagccgattt ataacggtgt
     1681 aaggcaggta agaacctttg aatatatagc aggctgtata gaaggcggaa gaaccggatg
     1741 cgcctgctat tcgcatcaag ggacggcatt gaaagaagtg acggagttga tgtgcaagga
     1801 ctatgtaaaa aacggcttgc cgtttaaccc atacaaagaa gaaagccaag ggcaggaagt
     1861 tcagcaaagc gcgcagcaac attcggacag ggcgcaagtt gccacattgg gcggaaaacc
     1921 gtagcagaac ctaatgtacg ataattggga agaacgcggg aaaccgtttg aaggaatcgg
     1981 cgggggcgtg gtcggatcgg caaactgaag aaaacggcaa gagagaaaaa agacccgtaa
     2041 accgtttgaa tatagacggt ttacgggtct ttgtttcgcg caaagcaagg gctaaggcag
     2101 tcaggcagca aatcccgcaa tgtattaaaa cagacgcgta gaaatgccgg ctgcctttat
     2161 ccatcctcga aattgaatat catcctagcc gtatcaaggc tgtataaata aggaaaatac
     2221 caatgaatat aatcgggctg gacatctcaa aggacaccat agacgcaaca ttgcataaaa
     2281 caaacggaag tatccattac attaaattta agaataatga tgatggatta aaacagttta
     2341 gattgtggat aaagggaaac agaatcagaa aagtctatat cggcatggag gcaacaggca
     2401 tctattacga aaaggcagca gatatgcttt cttcctacta tactgtttac gttattaatc
     2461 ccttaaaaat caaggactac ggaaaaagca ggtttaaccg taccaaaacc gacaaagcag
     2521 attcaaacct gatagcagac tacataaaaa ggcatcaaga tacattgata ccgtatcaga
     2581 tacccaaaaa caaagcactg caaaaactga ttaaccttaa aaatcaatta catcaacatc
     2641 agaagcaaat taaaaaccgt cttcatagca ctgaagaaga cttcataagg aacatacatc
     2701 aagacttgat agataccata caggacaaga tggaacaggt aaaaatagcc atatccgaac
     2761 aaatcaaaaa acaaacggac aataaccatt accgcaatct tcaaaccatc ccgagcatag
     2821 gcaaagacac cgcatcagtt ctttatgcgc aactgacaga aaaacatttt aaaaccgcaa
     2881 accagtttgt atcctatgcc ggattaaatc ccgccatcat acaatcaggg acaagcgtaa
     2941 gaggtcgggg cagattgagc cgatacggaa acagacgatt aaaaagtacg ctgtatatgc
     3001 ccgccctttg tgcttaccgt tttaacgcat ttccgaaatt aataaataat ctgaaaaaag
     3061 cgggtaagcc aaagatggta atcatcgttg ccatcatgcg caaactggcg aagctcgcct
     3121 attacattgt taaaaccggc cagccttacg atgcggaaag acaccgattg aatcaataaa
     3181 attcaacaaa attaaacggt tacgcgaata tatttgtgta accgtgcatt tgcatatcgt
     3241 aaataaacgt aaataaaaat aacaatataa atcagtatat tgcaactttg ttttttattt
     3301 tgtgttgacg ggcaacatat ca
//
LOCUS       DAWISC010000188         3291 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWISC010000188 DAWISC010000000
VERSION     DAWISC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.188"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            83..1094
                     /locus_tag="WIW87_001874"
                     /pseudo
     CDS             83..1094
                     /locus_tag="WIW87_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1310..2647
                     /gene="ribB"
                     /locus_tag="WIW87_001875"
     CDS             1310..2647
                     /gene="ribB"
                     /locus_tag="WIW87_001875"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ5174986.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALEHIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2807..2944)
                     /locus_tag="WIW87_001876"
     CDS             complement(2807..2944)
                     /locus_tag="WIW87_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ5174987.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(2955..3185)
                     /locus_tag="WIW87_001877"
     CDS             complement(2955..3185)
                     /locus_tag="WIW87_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ5174988.1"
                     /translation="MEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKM
                     AGFSADLDKLFAESAELEKEIKDRLAMLKFNS"
BASE COUNT          861 a          901 c          732 g          797 t
ORIGIN      
        1 ttacagggtg cggatgcaaa cgcattgcga gcgcgggttt gaggcatacg cgcaaacatc
       61 ttaatataat ggattgatat ttatgatttt ctccatcatc gtccctattt acaatgtgga
      121 aaatacctcc gctgctgcgt ggattccgtg cttgccgaaa attttgccga ttatgaaatg
      181 attttggtcg atgacggttc gccggacggc tgcgggaaga tttgcgacga atatgcaggc
      241 aaatatccgc atataaaagt gattcatcaa gaaaacggcg ggctgtcgga tgcccgcaac
      301 gccggtatcc gggcggcaaa aggcgattac ctaatctttt tggacagcga cgattattgg
      361 gccgatacca accgttcaaa aaacgcgggg gggggattct ctttgattta caacaacttg
      421 cagacaaaaa ggttgatttg atcctgcatc cctcgtcctt caattaccgc gacatcccca
      481 aaggggcgga cttttcggat aatgattttg tccgccattt tgaaacgctg gtggaggggc
      541 ggtactatat cgccaacgcg tggacaaaga ttgtcaggcg ggaaatcatc attaaaaaca
      601 atctgttttt cccaaaagga tacattcacg aggatttccc gtacagtttg caattggcgc
      661 gttttatcaa gacttttgcc ttttacgata acccttttta ccagtaccgc gttctcggcg
      721 gctctatcag ccacaacatc aaatacaaaa atttcagcga tgtgctgacg catctcgact
      781 ggggtgtgga ttttttagtc gaaaacaaaa attcccccat ctacggcggt ttgcaaaaat
      841 ttgtcttcga caatatcggc tatctgaggt ctatattggt aaggctttat ttttccaaaa
      901 acattatcct catctaccgg aaatattttt catttaaaga aaaatgcaga aagatattcg
      961 gcgcgaaggc aatccgtccg gtttttatcg ggaagaccgc attcatcata ggattgccga
     1021 tattgcgcct gctcgtaccg cctatgctgt acccggcaat caaggccgtt tatcagaaat
     1081 ttttttcgga ataagcttcc ggcaaacccc gaatcggaag cgggcgggaa gaaacagccg
     1141 ccccgccggc ggggattgcg gcaatgccgt ctgaagccac gaatccggct tcagacggca
     1201 tctatttacc aaaaagcaaa taattcggtt tggcgaaaaa aacagatttg ctttttggta
     1261 aatacgcgat tacaatccgc tacatccgat ttctacaaag gatgaaacga tgaccgacac
     1321 agccggtctg cgccgccaca acctgcggca gtggatagaa aaatactacg gcggtttgca
     1381 aactcgtttt gctgaagccg ttgccctcaa cacaggcgaa ctctccgccc ttttgaaaaa
     1441 caaatccttc ggcgagaaaa aagcccgtaa aatcgaacag gcggcaaaaa tgcccgcctt
     1501 ttggctcgat accgaacaca ccgcccgccc gtccgaacac acaggaaaac acaccatgtc
     1561 ccatatctcc cccatccccg aaatcctagc cgacatcaaa gccggcaaaa tggtcatcat
     1621 caccgatgcc gaagaccgag aaaacgaagg cgacctgctg atggcggcgc aattcgtcac
     1681 gcccgaagcc atcaacttca tgatcaaaca cgcgcgcggc ttggtctgcc tgccgatgga
     1741 cggcgaaatg gtcgaaaaac tcgggctgcc gatgatgacc caaaaaaacg gcgcgcaata
     1801 cggcaccaac tttaccgtct ccatcgaagc cgcacacggc attaccaccg gcatttccgc
     1861 cgccgaccgc gccctgacta ttcaaaccgc cgtttccccg accgctaaac ccgaagacat
     1921 cgtccaaccc ggtcatatct ttccgcttcg cgcccaaaaa ggcggcgtac tcgtccgcgc
     1981 cggacacacc gaagccggcg tcgacctggc gcaaatgaac gggctgattc ctgcctccgt
     2041 tatttgcgaa atcatcaacg acgacggcac gatggcgcgt atgcccgaac tgatgaaatt
     2101 cgccgaagaa cacaagctca aaatcggcac gattgccgac ctcatcgaat accgcagccg
     2161 taccgaaagc ctgcttgaag acatgggcaa tgcgcctgta caaaccccgt ggggcgagtt
     2221 ccaacaacac gtttacgtcg acaaactctc cggcgaaacc cacctcgccc tcgtcaaagg
     2281 cacgcccgcc gccgacaccg aaaccctcgt ccgcgtccac gaacccttca gtgtgatgga
     2341 cttcattcaa gccaacccgc gccattcatg gtcgctgccc aaagcccttg agcacatcca
     2401 acaagccgaa agcggcgtcg tcatcctctt acaccgcacc gaagacggcg catccctgct
     2461 cgaccgaacc ctacccaaag gcgcaaacca agcctacaaa tgggacagca aaagctacgg
     2521 catcggcgca caaatcctcg ccggcctcaa cgtcaaaaaa ctgcgcgtcc tcgggcagcc
     2581 ctcatctttc accggcctga ccggcttcgg tttggaagtc gtcggctttg aagaagcgga
     2641 aaaataatat agtaaattca aatactttat atttgcttta tttattgcat tatttccgtg
     2701 caaacgaaaa cccggtctgt tgggttggat tttgtttttt caaatttcgg gtaacttcta
     2761 attcgtcatt cccgcgctgg cgggaatccg gttcgtcggg tttttgtcat ttccgataaa
     2821 ttcctgtggc tttggttttt tggattctct ctttcaggga aagaacggca taagtatttt
     2881 ccaaaccaaa caaaatgccg tctgaaaggc tttcagacgg cattttaagt ttgaccggtt
     2941 tcattcatcg gtatttatga attgaatttc aacatcgcca atctatcctt aatctctttt
     3001 tccaattcgg cagattcggc gaaaagttta tccaaatccg ctgaaaatcc cgccattttt
     3061 tgcgcaaatt cgtcggcgga aatatccaca taatcaatct ttacctcgaa atactgcccc
     3121 gccgacaagc tgtgattctt cgctttgatt tcatcgtagc cgattaccac actgaaatct
     3181 tccactgcct gtttgtgcgt gaaggtattg cagatttttt tgttcttctt cgcgggaaag
     3241 tacggttttt ttgccgtctt taattttttc gcccaagccc gatgcgtcga t
//
LOCUS       DAWISC010000189         3247 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWISC010000189 DAWISC010000000
VERSION     DAWISC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.189"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(160..2292)
                     /locus_tag="WIW87_001878"
     CDS             complement(160..2292)
                     /locus_tag="WIW87_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ5174989.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          933 a          877 c          721 g          716 t
ORIGIN      
        1 agatgagtga aaaagaagtg caggctgcct aaaaagacag aaaaagtctt tccggcagcc
       61 tgcactttgg tttcatttca gtcagtaaac ccagtaaacg acggtctgaa aacgcagaac
      121 gttacgaaaa aagcagccta cacgcccatc ccccgccttc tacccgttct gtaaatcata
      181 cagatagcgg taatatccgt tcggcttcgc cagcaattcc tgctgtgttc ccgcttccac
      241 aatcctgcct ttatccatgg caatgatccg gtgtgccgtt ttaacagtgg acagacggtg
      301 ggcgataatc agcaccgtcc ggttggcgca aatggcctgc atgttctgca taatcgctcg
      361 ttcactttca taatccagcg cgctggtggc ttcatcaaaa atcagaatgc gcggattggt
      421 gattaacgcg cgggcaatcg caatacgctg ccgctgtccg cccgacaagc cggccccttg
      481 ttcgcccacc acggtgccgt agccttccgg cagctccata ataaactcgt gtgcgcccgc
      541 cagtttggct gcttcgataa tgcgttccag cggcataccc gtatccgtca gcgcgatatt
      601 gtcgcgtatg ctgcggttga gcagcacatt ctcctgcaag accacgccga cctgccgccg
      661 cagccaggca ggagcggcca aagccaaatc gttgccgtcc accaacaccc gtccctgctc
      721 cggtacatac agacgctgca ccaatttggt gagtgtggat ttgcccgacc ccgaacgtcc
      781 cacaatcccc agcacttccc ccgcccgaat ccgcaggttc aaatcctgca aaatcagcct
      841 gccgtccgcc ttatagcgga aatcgacatg ttcgaacgta atctcccccc ggatatcggg
      901 caaagccaaa tgcgaagacg cattctcggt cggcgcattc agaatatccc ccaaacgcgc
      961 caccgaaatc cccacctgct ggaaatcctg ccacaactgc gccaaacgga taacaggcgc
     1021 cgccacctgt cccgagagca tattaaacgc aatcagctgc cccaccgtca gcttgccctc
     1081 aattaccagc cgtgcgccaa tccacaacgt cgccaccgtc accagcttct gaatcagctg
     1141 caccccctgc tggccgacca ccgccaactt cgttacccga aatcccgaag ccacataagc
     1201 cgccaactga ttgtcccaac gctgcgtcat ctgcggctcc accgccatcg cctttaccgt
     1261 acccaccgca gtgatgcttt ctactaaaaa cgactggttg tctgcattgc gcgcgaactt
     1321 atcgttcaga cgcgtccgca gtatcggact gataaatgcc gaccaaaacg cataggcagg
     1381 caacgaagcc aataccaccc aagtcagagt ggagctgtaa taccacatca ccgccagaaa
     1441 gataaacgaa aacgccaaat ccaacaccga agtcagcgcc tgaccggtca agaaattgcg
     1501 aatctgctcc aattcccgca cccgagccac cgtatcaccc actcgtctgt gctcgaaata
     1561 ggataaaggc agggaaagca gatgccggaa caaacgcgcg cccaattcca catcaatacg
     1621 tgaagtcgta tgtgcaaaca gatacgtccg caaaccgccc aacacaatct caaacagcga
     1681 caccaccaac aaagccaccg acaccacatc caaagtagag aatccccgat gtaccagcac
     1741 cttgtccatc accacttgga aaaacagagg cgtaatcagc gcaaacagct gcaacaccac
     1801 cgacaccacc aatacttcaa aaaacaaccg gcggtatttg attaccgccg gaataaacca
     1861 ggtaaagtca aactttgcca aactgcccaa taccgaagcg cgggaagcaa ccaatatcag
     1921 tttgcccgaa tatctgttag aaaattcggc aaaagacaat accgcagact tattcgtagt
     1981 taaatcctgt attagatatt gggcatgctc acccccaccg tctgttttag ccaaaataaa
     2041 atggttgccg tcatcacacc ataccaatgc gggtaaagtc gccatagcca aacgtttaat
     2101 aggctggcgg actacctttg ccttcaatcc caaagatttg gcggctaaca gccattgcgt
     2161 ttcatttaaa tcgctctgtg cggaagtaca aaattcatgc tgtatatcgg caggattggc
     2221 ggcaatgccg tggtaatggg cgaggatgat gagggcggaa agggcgggga gcggtgcgga
     2281 tacgatagac ataaataaaa tatagttaga ttggatgtgg ataacggctg gctggaaaag
     2341 gaatatatta agtagaaaga aatatataaa taaaacagca gaacgcattg taaggatata
     2401 tatgggaatt gtaaagagaa agtatggaaa agttctcgtt tcaggaaggt aaaacggctt
     2461 aggaatcgag ttagatgagg atgcctcgca cctctcgtgc ctcctgcata ccgttaagga
     2521 acagggttaa ggtgcaggct gctccgaact ctgttgcggt cggataatgt tattttttgt
     2581 gtttcaggca gcctgaaata tctgtatatt tttgttttaa atagatttta aagattgata
     2641 actgttcttg acgatttttc aagaaaggag taaagtgact tattatcaat gacaagcaac
     2701 gcgcgaagtg acaaggaaaa ctatctactt aaattctaag gaggcttcga atatcataaa
     2761 ccaatcagaa acatagagat aaaaattatg tacaaatata atcctcttat acaatttatt
     2821 gcacagttga ttatgtctta tggagcaagc gtagggtggg cacttgctgc ccacgcgttt
     2881 catatttcaa ggcagcctga aactgtgtgg gcataatgcc taccctacat cccaaaaaaa
     2941 caagcgcagc ctgcgtgtgt agggtgcgaa ctttcggcag gcagacacgc ggttttatag
     3001 tttcaagctg agttgggatg cttaagataa agtacaaaac cttaaaaaat aagggggctg
     3061 tactagatta gccctaaatt ccacaccaat cccgcaaaat ttttagctgt cgggacggtg
     3121 tgccgaagtt aaatcgaaat tcgcattctt tcaagaacag cgggaaagat ttgcgatcaa
     3181 ttccgttcta tttgcgcaag acgcgttttg cctgattcca aaagttctca atgccgttaa
     3241 tgtggtt
//
LOCUS       DAWISC010000190         3188 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWISC010000190 DAWISC010000000
VERSION     DAWISC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.190"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIW87_001879"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIW87_001879"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ5174990.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1338)
                     /locus_tag="WIW87_001880"
     CDS             complement(661..1338)
                     /locus_tag="WIW87_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174991.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            1492..1576
                     /locus_tag="WIW87_001881"
     tRNA            1492..1576
                     /locus_tag="WIW87_001881"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Leu,seq:tag)
     gene            2096..3130
                     /gene="purM"
                     /locus_tag="WIW87_001882"
     CDS             2096..3130
                     /gene="purM"
                     /locus_tag="WIW87_001882"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ5174992.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          806 a          847 c          793 g          742 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg atgccacatg gttcaacatt
      661 tcagacatct tcactcccat cttttccggc agacataccc aacagcagtt tcaaaggttc
      721 ggacaaagcc aaggcaagag ccacccattc cacctgtgct gccgtacccg cacactctgc
      781 gcattcaaat tccacatgaa ccacgcccaa cacaccgcca ctttccgtgc agaccggaat
      841 ggaaatttgc gccgccgaag catgattgcg ttctcccgaa agctccccca aatccaacca
      901 acggcgtaca tccgaggcaa cattcatcca accgctttgc gccgaacggc aagccagcga
      961 aaccttgcct gccgcttcat ccaaatccca caggttttcc aaaacctcgc cctgtcggga
     1021 caggcatatc agttggaaag cctggttttc agatggcatc aaggcataga ccgccgcact
     1081 ccgcacgcct gtcgagcgcg aaaacaccga atccaaagcc atgaaaagcc gtttcagtgc
     1141 atcttcgcgg acattgtcgc acgacaggta atcggcaagt ttccaaccct catcactgcg
     1201 ccacaaaacc gaacggtcta tcgaagccgt ccccatatcc attaccgtct gcgccgtcag
     1261 atacgccgcc cgaacctcgt caagcggcag cttcaaaccc tgagtcagca gaaaatcctt
     1321 aatcaaagca gcaggcatac ccaaattccg tcaataaaaa caaaaaaccg cccgattccg
     1381 atgtcaggcg gacgaaaaac agattttacc gccccaaacc ccaagccatc aacagccccg
     1441 ccgaaaacct tgcacaaaaa aacaaaatct gtataatcgt ccaattccca tgcggacgtg
     1501 gcgaaattgg tagacgcacc agatttaggt tctggcgccg agaggtgtga gagttcgagt
     1561 ctctccgtcc gcaccaataa tgataaataa attatatagt taatgcttat cgggacaaat
     1621 tagtgccaca aaacataagt cgtctgcttt tcagcgggcg gcttttttat ctgcctatat
     1681 gtttcaacac gcagaacggt acataaagtg ccgccatttg ttccgcaccg aatcggaaac
     1741 gggtgcttcc gcacgcaatc agattcacca cccctaatgc cggggtttca accggaaaga
     1801 agaaaatatt gattttgatg atggcatcaa aaaacccctt ccgctcgtct gccaaaagga
     1861 atggttaaga aaagcaatgc gtcttttgat ggaacgcgat atataaggaa ttgaggggaa
     1921 gggcaggcgg gtttctgccc gttaatcctg cggacggtcc cgccaaaccc gacacgcgcg
     1981 ggcatatggc ggactgttta caatattgtc taaaaatctg aacctgcaaa cgtgaaataa
     2041 ggtagaatac gccccgttat tttaccgtcc aatccccatt taccaaggaa aaacgatgag
     2101 tacttcattg agttaccgcg atgcaggtgt cgatatcgac gcaggcgacc aactggtcga
     2161 aaacatcaaa ccgtttgcca aacgcacgat gcgcccggaa gtattggggg atttgggcgg
     2221 ttttggcgca ttggtcgaaa tcggcaagaa atatcaaaat cccgtattgg tcagcggtac
     2281 ggacggcgtg ggtaccaagc tcaagcttgc ctttgattgg gataaacatg atacggtcgg
     2341 catcgacctt gttgcaatga gtgtcaacga tattttggtt caaggggctg agcccttgtt
     2401 tttcttggac tattttgcct gcggtaaatt ggatgttccg cgcgcgaccg atgttattaa
     2461 aggcattgcc caaggttgcg aagaatccgg ttgcgccctg attggcgggg aaactgccga
     2521 aatgccgggt atgtatcccg tcggagaata cgatttggcg ggttttgccg tcggcgtggt
     2581 ggaaaaagag aatgtcatta ccggccgcag catcggcgta ggcgatgtgg tattgggttt
     2641 ggcttccaac ggcgcacatt caaacggcta ttcccttatc cgtaaaatca tcgaacgcga
     2701 caatcccgat ctggatgccg agtttgataa tggcaaaacc ttgcgggagg ctgttattgc
     2761 gccgacccgt ctgtatgtga aaccgattct tgccgctttg gaaaaattta ccattaaagg
     2821 tatggcacac attaccggcg gcggcattac cgaaaacgtg ccgcgcgtgt tgcctgaaaa
     2881 cacggttgca caaatcgatg ctaaatcgtg ggaattgccc aagctcttcc aatggcttca
     2941 aaaggcgggc aatgtggaaa cccaagaaat gtaccgaacc tttaactgcg gcatcggcat
     3001 ggtcgttatt gttgctgccg aagatgccga tgcggttcag ggtctcttgg gtgaacaagg
     3061 tgaaacagtt taccgtttag gtttgatccg tgagcgtcag ggagacgagc atcaaaccca
     3121 ggttgcctga ttgcttctat agcgaaatgc cgtctcaaac atgggtttgg acggcatttt
     3181 tatagagg
//
LOCUS       DAWISC010000191         3185 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWISC010000191 DAWISC010000000
VERSION     DAWISC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.191"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(63..2225)
                     /locus_tag="WIW87_001883"
     CDS             complement(63..2225)
                     /locus_tag="WIW87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5174993.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEEKNGQKVDK
                     PMEQQMKDRADEDTVHAYKLSNPTKTDTGVYVEAIHDIGDFTLTGGLRYDRFKVKTHD
                     GKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQN
                     PNLEIGWRGRYVQKATGSILAAGQKDRKGNLENVVRKGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFNKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          687 a          706 c          879 g          913 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcacgtc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac ttgttgaaca cgttgttaac cgaaagattg
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caagttgcct ttgcggtctt tttgacctgc cgccaatatc
      301 gaacccgtag ctttttgaac ataacggccg cgccagccga tttccagatt cggattttgg
      361 aagcggtagg caagggaggc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gctgacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggacg gcgacagagt cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ctgccagaag tagctgccgt ttgcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg cttttgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttacc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcat gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagcgtgaaa tcgccgatgt cgtgaatggc ttcaacatat
      961 acgccggtat cggttttggt cgggttggaa agtttgtagg cgtgaacagt gtcttcatct
     1021 gcacggtctt tcatttgttg ttccatcggt ttatcgactt tttgaccgtt tttctcttct
     1081 gtcgtcggga tggagaattt cgagttcaaa aatgcttgcg gtttgatttc ctgatggcgg
     1141 tagttgatac cgtatttcaa cagggtttgt tcggcaaggc ggctgtcgaa gttgaagttc
     1201 gcaccacgag tggtgattcg ggtatggttg gggcctttta cattgcctgc gtagccggtg
     1261 ccgctgtcat cggcggaata gcgttctttt tccaacacat aggcgttggc atccagtttt
     1321 tcgacaaagc ccaggttttt acccgtgtac gccaagttgg tgttggattg ggtagtttcg
     1381 cggtaagcag gggcttggcg gtcaatattt atccgtgaac ttttgtcgcc gacggtaaat
     1441 tcttcacgca cagtgcggat gccccggtgt tggtctttca tgtggctcaa cacgatgcgg
     1501 tggtcgtcgt cgccgaaggt tgttccgatt ttggcgaggt agctgcgttt gtccagcgcg
     1561 ctgtacggta cggttttgcc gccgttgaca ttgcggaaac ctttgccggc ttcgtaatct
     1621 ttttcatcgt tgcggttgta agagaacaag ccgtcgaagt tgccctcttt tccgaatacg
     1681 cttgcgccgt agcttacgcc ttcgttgctg gcaaagccgc tgttgaggcg cacgccccag
     1741 tttttatcca agcctttgag caggtcttgg gcatcgacgg ttttggcgat gatcgcgccg
     1801 ttggtcgcgc cgataccggc agaggcggaa cccgcgcctt tttgtacgga aacgacttta
     1861 accaaagcgg gatcgacaat aaatctgcct tggtggtaaa ggatttggct gtcggaatag
     1921 gcgttgtcca ccttgatgtc gacagagttc tgacccatgc cgcgcagcgt caggaattgg
     1981 gacgtgccgt tgccgccgcc gaaatcgatg gacggctctt cttttaagag ttcgcgcata
     2041 tcggttgcgg tgctttcgtc tttttgttgc agcgtaacga tgttggtacg gattttgctg
     2101 ccttggcggt cgccttttac agtaacggta tccagtacga ccttggcatt attttctgcc
     2161 gcgtgggcaa aacctgccgc aagtgtgagc gagagcaggc tgagacggaa caatggggta
     2221 ttcattcaat cgtcctcttg agtatgaagg gaagtaaatc caaaccgtta agatttggca
     2281 ggataagaaa aataattaat aattattttt atttatatta acaggggggg gggggatttt
     2341 tgcaaagctg attatcgttt ttatttgcga agtgttgttt tttgttgaca ggttttgtcg
     2401 gaaatgtaaa aaaacggcgg gaatatagtg gattaacaaa tgcgggaatg acgaagcctg
     2461 cgcgggaatg acggcggagc ggtttctgtt ttttccgata aattcctaaa acttaaaatt
     2521 tcatcattcc cgcaaggaca gaaaaccaaa aacagaaacc taaaattcgt cattcccacg
     2581 aaagtgggaa tctagaatcc cggactttca gataatcttt gaatattgct gttgttctaa
     2641 ggtctagatt cccgcctgcg cgggaatgac gatatttctg tttttgattt tttgtttttg
     2701 gggaatgacg ggatttgaga ttgcgggcat ttatcgggta aaacggaaat tatgcgttac
     2761 gaaaatttat ccgaaatcac ggcaactttt ccaccgtcat tcccacgaaa gtgggaatcc
     2821 aggtctgtcg gcacggaaac ttatcgagaa aaacggtttc tttatagtgg attaacaaaa
     2881 atcaggacaa ggcgacgaag ccgcagacag tacggaaccg actcactcgg tgcttcagca
     2941 ccttagagaa tcgttctctt cgagctaagt caaggcaacg ctgtactggt ttttgttaat
     3001 ccactataga ttttacgtcc tggattcccg cctgcgcggg aatgacgaat ttcaattttc
     3061 tgtttttgat tttttgtttt tacaggaatg acggtctttt catatcgaaa aaagttgccg
     3121 taccgcaccg ataatttccg cctgcgcgga atgaagattt aagcgttgcc cgaaattcaa
     3181 aaaaa
//
LOCUS       DAWISC010000192         3107 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWISC010000192 DAWISC010000000
VERSION     DAWISC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.192"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            11..1353
                     /locus_tag="WIW87_001884"
                     /pseudo
     CDS             11..1353
                     /locus_tag="WIW87_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1398..1688
                     /locus_tag="WIW87_001885"
     CDS             1398..1688
                     /locus_tag="WIW87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174994.1"
                     /translation="MNIQLQGHIVGVKKINGQIEGKSFDYCCLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1693..1890
                     /locus_tag="WIW87_001886"
     CDS             1693..1890
                     /locus_tag="WIW87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174995.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1898..2182
                     /locus_tag="WIW87_001887"
     CDS             1898..2182
                     /locus_tag="WIW87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174996.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            2189..2467
                     /locus_tag="WIW87_001888"
     CDS             2189..2467
                     /locus_tag="WIW87_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174997.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            2595..2912
                     /locus_tag="WIW87_001889"
     CDS             2595..2912
                     /locus_tag="WIW87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ5174998.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            2896..>3107
                     /locus_tag="WIW87_001890"
     CDS             2896..>3107
                     /locus_tag="WIW87_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5174999.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRA"
BASE COUNT          950 a          680 c          703 g          774 t
ORIGIN      
        1 gattcgcacc atggaagata agcaagggat gacaaaggcg gttgccggcg tgatgacgga
       61 cgcgctagcg gacggcagga agccgacaac cgcttcaaat cttcccccct tatctaacag
      121 ggggggacag aaaccgaaac ggcaggcagg gttcaggaag tcttcgaatg ttacgaaacg
      181 tacataacgg acggtaaagg aaacctgtta ggcgttcctc ttcggcgcgg tgtatcagat
      241 tcggctttca ttgatcaaat tagcttttca tttcatgaaa aaaccttttt cgataaatac
      301 ggcgttcgtg taagtctttt ggaagacgaa gattttattc gcgccgcgtc catgctcgcc
      361 gaagaagttt tcggtttcgg tatctacaaa gaatccaaag gttcgggcgg tcgtttctat
      421 gagcgctgtt ggttgatggg ttcggaagac gccctatacg gtcgcgtcca ttttggcggc
      481 caacaaaata ccattctttt cgaactgacc ggcaccggtt gcggcgtcgc aaaagaaggc
      541 tgggaatcac gacttttcgc attcctgact aatgcaatcc gcccaaaaat cacacgcgtt
      601 gacatcgcaa aagacttttt caacggcgaa tacagcccga accaagcccg tgaagaccga
      661 aataaaggta tgtttacctg tcatcacgtc aaaccaaaag gcgaatgttt ggggtcagat
      721 tgggaagaag acgatgaagc caaaatgacc aaaggcaaga cctatggtat cggctcccgt
      781 gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc agttgggcga taaaacaagc
      841 acatggacgc gatttgaaat tgaattcaaa gcaaaagaca tcgttatccc tttcgaagtt
      901 ttgcagaatc cgggcgaata tttcggcggc gcatatccga tttgcgaacg attcgcccaa
      961 aaggcaacgc gcatacacgc ggttaaggaa gataaggtca tttcagccga ccgctacctt
     1021 gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc tgaaattcat ttttcccgaa
     1081 ttggacaaag ccaaactgtt tgaactgatt gagccgagtc atcacaagct gcccaagtct
     1141 ttggctcccg aagcctacga ctgcgccttt ttgaaagctc aagccattca tgaacagccc
     1201 gcattcaaac cgtacaaaga cccttactat atgtacgaat attacgagaa tcttgaaaaa
     1261 cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct ataacaactt catttacgac
     1321 aaattcgcaa gactaccgat ttcatgggct taaagtgtct gcccgaaaga cgtttaatca
     1381 cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc gttaaaaaaa
     1441 tcaacggaca aatcgaaggc aagagcttcg actattgctg cctgattgtc gccacaccct
     1501 tagacagctc ccaaggcaac gcattgggca gctctactac tgaatacgat ttcggcggct
     1561 ctgccaattt cgagcagttc cgaaacgccc aatttccgat cgaagcaaac ctgaacgtag
     1621 aaatcgtcac tacgggcaaa acccaaaaac tgaaagtcat cggttttcaa ctcgtgaaga
     1681 aaggctgatt gaatgcagaa agtctatgtt gtccagtccg tatcaacagg ggactttctg
     1741 tatctctctc ctgaaacggg cgacatcgga cataccaaat taatcaccaa tgccgattat
     1801 ttctacgact tcgaagaagc gattaacgca ggtttggaag aaatcggcaa ccaatacgaa
     1861 tttgtcgtat tcggattttt gaaagactga ttttcggatg ttcggcggtc gtctgaaaaa
     1921 cgctccatcc attaccgcca aacacttttt gaaggaaaat atcatgaaat ttattaacac
     1981 ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct gctcccctgg ctttggccgc
     2041 acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct ttggaagccg caaaagcgga
     2101 cggtatggaa gccggttgga ttgtagtggg cattttcgcc gcgctttttg tattttccat
     2161 cgttaagaga gtgatgaagt aagacggcat gtactaccaa gtcggaaata aatgtcttga
     2221 gaagcaccag gctgaaaacc tttatttcag cttggtagta ccaagaatca aagaaaacgg
     2281 acagattgtc aggccggaat ataacggcag cctgtggaag atgtcggacg gtcagccgct
     2341 aaggctttta ttggcggaat gcagtccgaa agacaacctg caaagcggtc ttgaaacagg
     2401 ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt tccctgctga aaaaggtttt
     2461 gaaatgatgg atttttattt ttatctcggc gtttccgtac ccgtattaat cggggcggtt
     2521 ctgtttaaga attgagcgca tgaagttatg gtgtcaaaat caggctttca aaacaatcat
     2581 tgaaaggcag aaccatgaac aagccgttta tcactcaggc gcagttggca ctttataaat
     2641 atcagccgtc cagcaagtat tttgggcaat cgatggcggt tatagcgcaa tctgaatttg
     2701 ttgaatttgc gaagattaat aagtctgaaa atgttattga ttgtttctct tttttctgga
     2761 atagaagaat taaacatgat atttggctaa tctcattttc tgataattca gaaatggtaa
     2821 ttaaagaatc cctgaaagat ggtcataaaa tatacaaatt tgaattttgc gaaattgtcg
     2881 ataattgcaa ttttgatgat gtattcgttt gaagcgaatg caaatgcagt aaaaatatct
     2941 gaaactgttt cagttgatac cggacaaggt gcgaaaattc ataagtttgt acctaaaaat
     3001 agtaaaactt attcatctga tttaataaaa acggtagatt taacacacat ccctacgggc
     3061 gcaaaagccc gaatcaacgc caaaataacc gccagcgtat cccgcgc
//
LOCUS       DAWISC010000193         3101 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWISC010000193 DAWISC010000000
VERSION     DAWISC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.193"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..211)
                     /locus_tag="WIW87_001891"
     CDS             complement(<1..211)
                     /locus_tag="WIW87_001891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175000.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
     gene            271..894
                     /locus_tag="WIW87_001892"
     CDS             271..894
                     /locus_tag="WIW87_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ5175001.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WIW87_001893"
     CDS             1005..2387
                     /locus_tag="WIW87_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5175002.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="WIW87_001894"
     CDS             2391..2915
                     /locus_tag="WIW87_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ5175003.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          621 a          839 c          939 g          702 t
ORIGIN      
        1 cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg ctgcataatc cggacgacgg
       61 ataaccggtg aaaacggaaa tgcccgggcg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtagaattga ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgcgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgagaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgagcgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgcggg actggtcgtg tttgcggcgg gcagctttct tgccgccgcc gccgatacgc
     1321 tgcccatgct ggtcgccgca cgcgccatac agggtgcagg ggcggtcagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtttgacg ttttcggtca gcctcgtcgt tgcccccgtg attgccgacg
     1501 cggtcggcgt tcgcggactg tttatgctga ccggcattct gaccgtcatc agcatcggcg
     1561 tggtggcgtg gatgactccc gatcccgaag tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctt gatttcggca
     1681 ttttcgccct gcacgccgca caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccttcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctt aagcaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatttgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggcgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct g
//
LOCUS       DAWISC010000194         2969 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWISC010000194 DAWISC010000000
VERSION     DAWISC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.194"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            124..1668
                     /gene="purF"
                     /locus_tag="WIW87_001895"
     CDS             124..1668
                     /gene="purF"
                     /locus_tag="WIW87_001895"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ5175004.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1768..2259
                     /gene="greB"
                     /locus_tag="WIW87_001896"
     CDS             1768..2259
                     /gene="greB"
                     /locus_tag="WIW87_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ5175005.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRDDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2318..2944
                     /locus_tag="WIW87_001897"
     CDS             2318..2944
                     /locus_tag="WIW87_001897"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ5175006.1"
                     /translation="MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAATRFPDAQALLFDAYHPSEYGGTGNRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAESVDVSGGVEASKGKKDAAKVAAFIATANRLSR"
BASE COUNT          713 a          905 c          776 g          575 t
ORIGIN      
        1 tcttattctc cataaaccca cctatctgtc cttcgctcaa atattgtcaa caattcaggc
       61 aaaaaaccac cgtttacttt ataattccgt tttaccctta aacctcgaat cggagaagac
      121 gatatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac
      181 ggcttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc
      241 ggtaccttcc atatgcacaa aggcaaaggc atggtgcgcg aagtgttccg cacacgcaat
      301 atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc gttatcctac tgcgggcaac
      361 gcaggcagca gcgcggaggc gcagcctttc tacgtcagct cgccgttcgg catcgttttg
      421 gcgcacaacg gcaacctcac caacactgcc gaactgtatg aaaacgtgtg taacaaacac
      481 ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc tcaacgtatt cgcgcacgaa
      541 ttacgccgcg aagtctctaa aaacgccgac ccgcaccggc tcaatgccga caatattttc
      601 aacgccgttg cccaagtcca ccgcctggtg cgcggcgcat acggtgtgat tgccatgatt
      661 gcgggctacg gtatgctcgc cttccgcgac ccttacggca tccgcccgct ggtattgggt
      721 tcgcaaaccg acagcgaagg cagaaaatcc tatgccgtcg cctccgaatc cgtcgccttc
      781 aacgcgctta cctacgattt ggaacgcgac atccggccgg gcgaagcggt atttgtcggc
      841 tttgacggca caatgattgc ccgccaatgc agcgaccgtg ccaaactcag cccctgcctt
      901 tttgaatatg tttattttgc ccgccccgac tctgtgattg acggcgtatc cgtttaccaa
      961 tcgcgcttgg atatgggcgt gtcgctggcg gaaaaaatca aacgcgagct gcccgtggac
     1021 ggcatcgatg tcgtgatgcc cattcccgac accagccgcc ccagcgcgat ggagcttgcc
     1081 gtccatctcg acaaacccta ccgcgagggt ttgattaaaa accgctatat cggccgcacc
     1141 tttattatgc ccggacaggc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg
     1201 gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcgggacg
     1261 accagccgcg aaatcgtcga aatggtacgc gcggcgggcg cgcgcaaagt ctatatcgcc
     1321 tccgccgcgc ccgaagtgcg ctatcccaat gtgtacggca ttgatatgcc cacgcgcgaa
     1381 gagttgattg ccaacgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc
     1441 atcgtatttc aagacttggg cgatttggaa gccgtcgtca aagcactcaa tccgaaaatc
     1501 gaatccttcg attcgtcctg tttcaacggc atttatcaga ccggcgacat cgatgatgcc
     1561 tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg gtctgaaaat ccacccgagc
     1621 aggatggaac acagcatcag catcagcgat gcgggcgacg aagaataagg cttcccaaac
     1681 cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg gcatttccca tatcggcatc
     1741 acatttcccg tttcatccgc acacaatatg agtaccgaaa ccaaaaacta cattacgccc
     1801 gccggatggc aggcattgaa agacgagctt tatcaactgg tcaacaaaga acgccccgaa
     1861 atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc gcagcgaaaa cggcgactat
     1921 ctttacggca aacgccgtat gcgcgaaatc gaccgccgca tccgtttcct gaccaaacgt
     1981 ttggaggctg ccgtcgtcgt cgatcccgaa ttgcgcgaag cgaccgacca agtgtttttc
     2041 ggcgcgacgg tcggactgtt gcgcgacgac gggcgcgagc aaaccgtcaa aatcgtcggc
     2101 atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga tttcgccgct ggctcgttgt
     2161 ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca cgccggaagg gcgtgaggaa
     2221 attgaaatcc tttctgtcga atacatcaaa atcgactgat cgggaatgcc gtctgaaacg
     2281 gcaaaagcgc gccgtttccc aaataaagga ataccgaatg aggaaaatcc gcaccaaaat
     2341 ctgcggcatc accacaccgg aagacgcagc tgccgccgca gcggcaggtg cggatgccgt
     2401 cgggctggtc tttttccaag gcagcagccg ggccgtcgat attgcccgcg ccaaaaaaat
     2461 caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt ttcgtcaacg aaagcgcgca
     2521 aaacatccgc cgcatccttg ccgaagtgcc gatacacatc atccaattcc acggcgacga
     2581 agacgacgca ttctgccgcc agttccaccg cccctatatc aaagccattc gtgttcagac
     2641 ggcatcagac atccgaaacg ccgccacgcg cttccccgac gctcaggcac tgctgttcga
     2701 tgcctaccat ccttcggaat acggcggcac cggaaaccgc ttcgactgga cgctgctggc
     2761 ggaatattcg ggcaaaccgt gggtgcttgc cggcgggctg acccctgaaa acgtcggcga
     2821 agccgtccgc atcaccggag cggaatcggt cgatgtatcc ggcggtgtgg aagcgtctaa
     2881 aggcaaaaaa gatgccgcca aagtcgccgc ctttatcgca accgccaacc gcctatcccg
     2941 ttaaagcaac aaaaattgcc gccggaatg
//
LOCUS       DAWISC010000195         2945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWISC010000195 DAWISC010000000
VERSION     DAWISC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.195"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..317
                     /locus_tag="WIW87_001898"
     CDS             <1..317
                     /locus_tag="WIW87_001898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175007.1"
                     /translation="KNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGF
                     NGDIGEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEM
                     ER"
     gene            complement(399..1970)
                     /locus_tag="WIW87_001899"
     CDS             complement(399..1970)
                     /locus_tag="WIW87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ5175008.1"
                     /translation="MLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGL
                     LTILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSI
                     DHIQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLR
                     NATEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLS
                     ALFEFSNKLLFSMENVIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYI
                     QIKMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFE
                     NINLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGL
                     SGIVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDM
                     GNILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAH
                     RQETIQSADKILNLG"
     gene            complement(1980..2485)
                     /locus_tag="WIW87_001900"
                     /pseudo
     CDS             complement(1980..2485)
                     /locus_tag="WIW87_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815283.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(2488..2945)
                     /locus_tag="WIW87_001901"
                     /pseudo
     CDS             complement(2488..2945)
                     /locus_tag="WIW87_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
BASE COUNT          971 a          496 c          517 g          961 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgtg aaattagccc aaaaatggga acaagagcag aaaaaacaac
      241 aaattcaaca gaaaaaggaa actgaaaaat cgccaaaaca caaagccagt cgtgatgatt
      301 gggaaatgga acgttaaacc gctattttca tgtattctat taaattattt gaaaaaagac
      361 ttcacttttt gttaaagaaa aatgaagtct tgttctgttc aacctaaatt cagaatttta
      421 tctgccgatt gaattgtttc ttggcggtgt gcaaccatta ttttcataat accaagactt
      481 tttaggttat ggttaatttt ttgttcattt tctacatcta aatggctact tgcttcgtct
      541 aaaaatagaa ttttgggtcg tttatacaat gcacgagcca agataactct ctgcttttgt
      601 ccacctgata agatatttcc catatcgcca atcaaggtct catagcccat tggcatttta
      661 agtatatcgt catgtatttg tgccattttt gcacattgtt caatgagctc catatttggg
      721 ctttcatcaa aaaatgaaat attttcccca atagaacctg caaaaaggac atcatcttgg
      781 cgaacaatcc cgctcaatcc cctaataaag gatggagaaa cttgatatat atcatgccca
      841 ttaatactaa ctgtaccagt ttcaggtttt aggctacctg ttaaaatgtt taacaaagtg
      901 gacttccccc gaccagattg tcctgttaaa acaactgctt cattatcttt aaattccaaa
      961 ttaatgtttt caaaaagata tggctcatta tcagcatatc tgaatgagac gttttcaact
     1021 ttaagaacca gttgttcatt atctaattta ggtatatgat tatacttaat aatttcagtt
     1081 tctgtttcat ttaaagtaat gtcagccaaa cgttcagcat gaagccctaa cattttgatt
     1141 tggatgtatt ggtcaacgag agaagctgtt ctgctttcaa attgcccttt ataagccaaa
     1201 aaagccatca gaacaccgac tgtaaatgaa ccatctaaaa ttgcgcttgc accaagataa
     1261 attatgataa cattttccat gctaaacaac agtttatttg aaaattcaaa taaagcagag
     1321 agtttatctg ttgtcagctt ggtattgact gtattcacaa ataggctcat ccaagtgcca
     1381 tgtctttgat aatgtttatc aaataattta actgattgga taccacgaat ggtttccatg
     1441 aaatatgagt tttgtttggc ttcatgaaca atattttctt ctgttgcatt tcttaatggg
     1501 taatatgcaa gccaacgaat tagtatgtac aaaacaagtg ttaaaagaac aatcagcgat
     1561 aattgagtgc tgtaaattgt cattaacacg aaagtaaaaa cagccattaa gctatttaaa
     1621 actaaaacaa aaaaagtaga agttagtgtt tcttggatat gatctattga accaaatctt
     1681 gaaatcacat ctcctaaatg tcgtttactg aaatagtcat taggtaagtc aagtaacctt
     1741 ttaaatatat tggctttcca ttgtaaatta agagttgtag ataggtgcat acctacccat
     1801 gcttgtaaca ggctaattaa ctgttgcagg atagtcagta aaccaaatcc caaagtaagg
     1861 gtcaataata aatttttatc agcagttaca atgacatggt ctattaccca ttgcataaag
     1921 aatggactaa ccaatgcaaa gacttccaaa gaaatagcta atataagcat ttgaattaaa
     1981 gagcgtttta agcctgaccc ccccttaata gagataatat tttgattttc tttgtttctt
     2041 ttttctcttc aaaatgggta ttggggaata attctagggc aatccctgtg aatttttgtg
     2101 aaacttcgtc cattttgatt tttcgcatac cgacagcagg gtccataatg acgatactgt
     2161 ctttggaaat ggaacaaagt acaacaaaat ggtttaagtt ccaatggaga atgcagggta
     2221 gttgtaaatt tgacagctca tctaactcta aacgcaaagc tcgtggcgtt aaattcattt
     2281 cattgccaaa tctcatgatg tctgcaagat ttgcgccctt taatgacagg gtgtattttt
     2341 ggcgtaacgt tcttaaatca gtgtgaaagc cataatagga caagatggat gtcaggcatg
     2401 ctaaaccaca ttcagcaact tctgtttgca gaatgacagg tagctttttg ttaaatccaa
     2461 aggacagtct tgataaataa tccattttta attgattttt cctgaaatgc tgtaaagtgg
     2521 gtcaagtacc cattcgtaca attttttcgt tcatggagaa tatctgcttc taaaatcatg
     2581 ccaatttgaa gcggcttgtt ttctccgtat gctttaatcg tttgtttttc caatttaact
     2641 ttcacaagat aggcaggttc atttaataag gttgggttag tgaaaatgat acctaaacct
     2701 gatagctttt gtttaccgag agcagttctg gcaactgaaa taatttctcc tgtggcatgt
     2761 ccaaattttt ggtaagggta cgcttggtaa cgtaaaacaa ctttatcttt cggtttaata
     2821 aaaccaacag ctttactggg tatgtaaaga ttggcgacca attcagtttg ttcagggaca
     2881 atgcttaaca gcaatttaga cggttcaact tgttgcccta tatcaacatt aattgttgat
     2941 atata
//
LOCUS       DAWISC010000196         2925 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWISC010000196 DAWISC010000000
VERSION     DAWISC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.196"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..806)
                     /locus_tag="WIW87_001902"
     CDS             complement(<1..806)
                     /locus_tag="WIW87_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin D"
                     /protein_id="HEZ5175009.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSG"
     gene            complement(820..990)
                     /locus_tag="WIW87_001903"
     CDS             complement(820..990)
                     /locus_tag="WIW87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175010.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     ILIIGIFLSIFF"
     gene            complement(2031..2267)
                     /locus_tag="WIW87_001904"
     CDS             complement(2031..2267)
                     /locus_tag="WIW87_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ5175011.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(2777..2925)
                     /locus_tag="WIW87_001905"
                     /pseudo
     CDS             complement(2777..2925)
                     /locus_tag="WIW87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          931 a          514 c          496 g          984 t
ORIGIN      
        1 cctgatttac tagctcgtat ggtttgttcg gatttcaaat caaaatccaa aatttcttgg
       61 ttcatttccg taatcgcacg gttgagttgg ctcaattcgg ttttatggcg ttcaggcagg
      121 ctgcttaatg tgattttctg ttcatcaagt tccctgattg cattattttg ttcacgtttt
      181 aggctctcca aacgtgagcg ttgttccaat aaatggcttt cggcggtcat cttatcttgt
      241 tgggatactg cgccttgact ggctaaaaac ttgttcttgt taagggtttt ttccgctaaa
      301 cgaatttgac gattttgccc tgtaatttgc tgtttaatat tctctaattg attgtttaaa
      361 cgatgaatgt tgttatgaac atttttttgc tcattttgat gaatgcgctt taaacgttcc
      421 aattcttgta atgccaaagt ttttttaagg ttggcttctg ctgccaattt ggcttgtacg
      481 tttccttttt cgccaaaacg cgatgtggaa agtttgaaca atggttcgcc agctttgaca
      541 aagttaccat cttcaacaaa tttatgcgta atcgtgccga tatcggaaga gtaaacacga
      601 accaccccca tagttggaag taattgacct tcaacggttg ttttattggt atagctacca
      661 aaaatcaaaa agataatgat acacagagca atgagaaaag cgcaaaaagt cagaaataaa
      721 aaagagaatg gacgggtcaa gataacctga cctgtccact tattttgttg ggctacaaag
      781 acttcttttc ggaataagtg ggacatttat tattatttct taaaaaaaaa tggatagaaa
      841 aataccaata attaaaatac ttatgcctaa taacataaat ttgctattga ttgatgtttc
      901 tattttaatt acattaaatt taaccaataa tccagaaatg gttgcagcta aaatgcaaaa
      961 gacagtggcg taaatggttt cagtattcat aatttttctc tttcaaaatt gttcaatcta
     1021 aatagaaaaa ggtaacttca atacatattg ctgtaactga aattaccttt tccttaatga
     1081 tgtaattatc ttatggcttc atcatgccac gaccgatatt gtatccgcca gcaacagaga
     1141 aaataccagc tcccattgcg tcaacccagc ctactggtcc tgtccacttg ttccgttgag
     1201 ctacaaagac ttctggtctg aaaaatgagt gattgttaga catgtgtact tatttatgtt
     1261 tctttgattg ggataaattt gtaaaaacaa aaaaaaggca agttgttatt atagaagaac
     1321 ttaaaatgct taacatactc attgaggttt gtaccacata atgagatacg agataatttg
     1381 ttgggagtaa aatcaaaaaa aagattaaat atttaaaata atcactatat tttttcatta
     1441 tttgttcctt tttgtattta agttcttatg aaaaactagg gtattttgaa tagaaaatat
     1501 caaaataccc tagttttatt aaggaatatt accaccaaca agtgcttcca tattgtacag
     1561 caccagtgat tgcaccacca atagctccga atttcgcacc tggaatagca ccaataccac
     1621 ctgcaaatga accaacaatt gccccaccag ctgctccacc taatgcacta ccaatggtat
     1681 tttttgagaa atcacgccag ttacaagcag caccagaaac ttgttaaatt tcattcaaag
     1741 tcaaaacttg cataatattc acctttgtta agtgtggttg agaaaaaagt aaattttcat
     1801 cgttactcat tatttgcaat catgcatata ctgtaaccaa gcactatatt cagttggatc
     1861 atagtttttc ctactgggtt gtctgatatg actattggca ctattggcac aattgggatt
     1921 agcaacttta ttgtgagtac ctaatggatt gatatttcct gtaaagtggc tattgcatgc
     1981 gcttaatgag catagattta ttaatgtcag aaaggataaa acaaaaaatc ttaccatttt
     2041 ttactaatac caaaagtagg tttcgaaaac tgtacaccaa gaccttgtac atatccaaat
     2101 tgcttgctaa cagaaaactc tactccacta ttgttagggg aataggaagc accaatttta
     2161 ccaacagcaa ccatatcttt tttacctagg ttgccgccac cctgtgcaaa gatatggaat
     2221 ttgccacctg acacttcaac taattcagag gtgtgtaact ctttcatgat tttctcctgt
     2281 aaagaagtat ggttggaaat gtaaatcaga attaaccttt cttaagttta atttaatact
     2341 ttttttgcaa gtattttatt ttaaattaaa tcaatatttt aaaaatattt acttgtatac
     2401 tgaaaaagaa actcctaatg gtggagtggg gcttttgttt aagtaagcat taaaaaaatt
     2461 taaatttccc attatgatta acttaatgca agagcttttt caggacgtac aaaccagcga
     2521 acgggcgttc tgatgtgggc agcctgccgg gaagtctgct ctattgttcg gacaataggg
     2581 ggaatatacc aattacttta actacatcat tttttgacat ttttcttgcc tttcaattta
     2641 aaatccaaaa tcatactgcc ataatttagc aatccaaaaa aatttaggca gcagtaatgg
     2701 tttcgtattt tagaaaacga aacttggttt ggtgttttga agcatttcag tacacaaacg
     2761 gggctgtcct agataactaa gataaactcg attttactaa ttgttttaaa atggaacaag
     2821 aacttttatc tcactgttgt taaaacgtcg ttcgcactcc tttaaataca gctcaaaatg
     2881 cgctttggga atgccgttag acttgcgtaa atgacgtttt gcctg
//
LOCUS       DAWISC010000197         2830 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWISC010000197 DAWISC010000000
VERSION     DAWISC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.197"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            32..463
                     /locus_tag="WIW87_001906"
     CDS             32..463
                     /locus_tag="WIW87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ5175012.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            474..1340
                     /locus_tag="WIW87_001907"
     CDS             474..1340
                     /locus_tag="WIW87_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ5175013.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAVRRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIW87_001908"
     CDS             complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIW87_001908"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ5175014.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2547..2636)
                     /locus_tag="WIW87_001909"
     tRNA            complement(2547..2636)
                     /locus_tag="WIW87_001909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2600..2602),aa:Leu,seq:taa)
     gene            complement(2739..2812)
                     /locus_tag="WIW87_001910"
     tRNA            complement(2739..2812)
                     /locus_tag="WIW87_001910"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Cys,seq:gca)
BASE COUNT          681 a          764 c          675 g          710 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaggcgaaca ggaccggcgc aaacggttgc tgtcgttgac
      301 cgaaacaggc aaagcctatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agtatttgcc acattcggcg acaagcgcac aactcggctg tttgccgatt tggatgcact
      421 ggctgaagtg atggaaaaaa caatctcgga aaataaaaaa taggggggca aatatgtgga
      481 aaatgttgaa acacatagcc caaacccacc gcaagcgatt gattggcaca ttttccctgg
      541 tcggactgga aaaccttttg atgctggtgt atccggtgtt tggcggccgg gcgatcaatg
      601 ccgtgattgc gggggaggtg tggcaggcgt tgctgtacgc tttggttgtg cttttgatgt
      661 ggctggtcgg tgcggtgcgg cggattgccg atacgcgcac gtttacgcgg atttataccg
      721 aaatcgccgt gccggtcgtg ttggaacagc ggcagcgaca agtcccgcat tcggcggtaa
      781 ctgcgcgggt tgccctgtcg cgtgagtttg tcagcttttt tgaagaacac ctgccgattg
      841 ccgcgacatc cgtcgtatcc atattcggcg cgtgcatcat gctgctggtg ctggaatttt
      901 gggtcggcgt gtcggcggtg ggcatacttg cgttgttttt atggcttttg ccacgttttg
      961 ccgccatcag cgaaaacctg tatttccgcc tgaacaacag cttggaacgc gacaaccact
     1021 ttatccgaaa aggcgaccgg cggcagctgt accgccatta cggactgctt gcgcgcctgc
     1081 gtgtgctgat ttccaaccgc gaagccttcg gctatctctg cgtcggcacg gcgatgggta
     1141 ttttgttcgg ctttgctttt gtgatgatga cgctcaaagg ctacagcagc gcggggcatg
     1201 tctattcggt cggcacttat ctgtggatgt ttgccatgag tttggacgac gtgccgcgat
     1261 tggtcgaaca atattccaat ttgaaagaca tcggacaacg gatagagtgg tcggaacgga
     1321 acatcaaagc cggaacttga aaaatgccgt ctgaacacgc ttcagacggc atttccatcc
     1381 gttcggcaaa ctacatcaca tccgcccgcc ggttgacaag tttggcaaac aacttttcaa
     1441 cagaagcttc cgcctgcaaa ccaatgcgct ggatcaggct ttgcttctcc tgatatttca
     1501 cttcgataac ctgtttgttt tcaaacgctt tcaacaacaa atcatcactg gtcgaaatct
     1561 cgtcaatcaa gttcaacgcc aacgcctgcc gaccgaacca atgctcgccc gttgccactt
     1621 cctcaatatc caattgaggg cggttctcgc tgacaaactg cttgaacaac tgatgcgttt
     1681 cctccagttc ctgtcggaat ttctgtttgc ccttttccgt attttcaccc ataaaagtaa
     1741 ccgtgcgctt aaattcgccc gccgtcatca catccacatc aatatcatgt tttttcaaca
     1801 ggcggtggat attcggtact tccgccacca cacccaccga accgacaatc gcaaacggag
     1861 cggaagcaat tttatccgcc acacacgcca tcatataacc gccgctcgcc gccaccttat
     1921 cgacggcgac ggtcagcgga atattgcgtt cgcgcaaacg cctaagctgc gaagccgcca
     1981 aaccgtaacc gtgaaccacg ccgcccggac tttccaatct gagcagaacc tcatcttcag
     2041 gcttggcaat caaaagcacc gccgtaatct catgacgcaa ggattctacg gcgtgtgcat
     2101 acaaatcgcc gtcaaaatcc aacacaaaaa ggcgggattt ttgcgtttcg gcagatttct
     2161 ccccaccctc cttcaaacgc tttttctctg ctttggcttc cgccttttcc tttttctttt
     2221 cctctttttc ctgatgtttt gcctcttccc cgcttaaaaa gaatgcttca aacgattgcc
     2281 gctgtttttt ataattttcc gaaaaatccg tcagtacgac actgccgctt tccgactgtt
     2341 tcttactctg tacgatagcc aacacaatca gcgcaattgc gccgaacacg gtaagcagtt
     2401 cgagcaggaa aataccgtaa ttcagtaaaa tttctttcca cattgattgg atttcctctt
     2461 gttcaggcat gaacatgtca atattgtcca tcaccgtccg acagataaaa aaataaccgc
     2521 ttggagcggc attgtcattt tcagcttggt gcccggagcc ggaatcgaac cggcacggga
     2581 tgtttagtcc cgacggattt taagtccgtt gtgtctacct atttcaccac ccgggcattt
     2641 gtgaaaggtg gaggcggggg cgcggatttt aaccggcctg tatgaagatt gcactcctca
     2701 tagcataaac actctgccac cccgccatag tacgataatg gaggcgagag tcggaatcga
     2761 accggcgtag acggatttgc aatccgctgc ataaccactt tgctatctcg ccctaaaact
     2821 ggcttatcta
//
LOCUS       DAWISC010000198         2580 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWISC010000198 DAWISC010000000
VERSION     DAWISC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.198"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            73..687
                     /locus_tag="WIW87_001911"
     CDS             73..687
                     /locus_tag="WIW87_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ5175015.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
     gene            848..2509
                     /locus_tag="WIW87_001912"
     CDS             848..2509
                     /locus_tag="WIW87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ5175016.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          505 a          650 c          668 g          757 t
ORIGIN      
        1 cggggttgaa gttgacatct acaaggttta atgtttgaat acatttaaaa atatatttga
       61 atgagatgtt ttatgattca aaatgtcgtt acttcaataa tcctgtattc tgggacagcc
      121 gtagacttac ttattatcct aatgttattt tttgccaaaa gaaaaagtag aaaagacatc
      181 attaacatct atttaggaca atttctaggc tctgttagtc tgatattgct aagtttactt
      241 tttgcatttg tcttagatta tattcctagt aaagagattt taggtttgct cggcttgatt
      301 ccaattctcc taggcatcaa agttttgctt ttaggagatt ctgatggaga ggctattgcc
      361 aaagagggtt tgcgcaaaga taataaaaac ctgatttttc tagtcgctat gattactttt
      421 gcaagttgtg gtgctgacaa tattggtgtc tttgtcccat attttactac cttaaattta
      481 gcgaatttga tagtggcttt acttaccttt ctagtcatga tttatctctt ggttttttct
      541 gcccaaaaat tagcacaagt cccttctgtt ggagaaactt tggaaaaata tagcagatgg
      601 tttgttgctg ttgtttattt aggattgggg atatatatcc tggttgaaaa caacagtttt
      661 gatatgctat ggactgtgtt gggctaggaa aaaatattat gaaaaagata gtatctgcaa
      721 agactgccat gtgcagtctt ttttgttgcc ggtctttttg tgtctgatgc cgtctgaagc
      781 agtctctgca cgacctttgt gcgaatattt gctacacttg gcaatccgtc cattttttct
      841 cacgcttatg ctgacctata ccccgcccga tgcccgcccg cccgccaaaa cccacgaaaa
      901 gccgtggctg ctgctgttga tggcgtttgc ctggttgtgg cccggcgtgt tttcccacga
      961 tttgtggaat cctgacgaac ctgccgtcta taccgccgtc gaagcactgg caggcagccc
     1021 cacccccttg gttgcccatc tgttcggtca aaccgatttc ggcataccgc ccgtgtatct
     1081 ttgggttgcc gccgcgttca aacatttgct gtcgccgtgg gctgccgact catacgatgc
     1141 cgcacgcttt gcaggcgtat tttttgccgt tatcggactg acttcctgcg gctttgccgg
     1201 tttcaacttt ttgggcagac accacgggcg cagcgtcgtc ctgattctca tcggctgtat
     1261 cgggctgatt ccagttgccc atttcctcaa ccccgctgcc gccgcctttg ccgccgccgg
     1321 actggtgctg cacggttatt ctttggctcg ccggcgcgtg attgccgcct cttttctgct
     1381 cggtacgggc tggacgctga tgtcgttggc agcagcttat ccggcagcat ttgccctgat
     1441 gctgcccttg cccgtactga tgtttttccg tccgtggcaa agcaggcgtt tgatgttgac
     1501 ggcagtcgcc tcacttgcct ttgccctgcc gcttatgacc gtttacccgc tgctcttggc
     1561 aaaaacgcag cccgcgctgt tcgcgcaatg gctcgactat cacgttttcg gtacgttcgg
     1621 cggcgtgcgg cacgttcaga cggcattcag tttgttttac tatctgaaaa acctgctttg
     1681 gtttgcattg cccgcgctgc cgctggcggt ttggacggtt tgccgcacgc gcctgttttc
     1741 gaccgactgg gggattttgg gcgtcgtctg gatgcttgcc gttttggtgc tgcttgccgt
     1801 caatccgcag cgttttcagg ataacctcgt ctggctgctt ccgccgcttg ccctgttcgg
     1861 cgcggcgcaa ctggacagcc tgaggcgcgg cgcggcggcg tttgtcaact ggttcggcat
     1921 tatggcgttc ggactgtttg ccgtgttcct gtggacgggc tttttcgcca tgaattacgg
     1981 ctggcccgcc aagcttgccg aacgcgccgc ctatttcagc ccgtattatg ttcctgatat
     2041 cgatcccatt ccgatggcgg ttgccgtact gttcacaccc ttgtggctgt gggcgattac
     2101 ccggaaaaac atacgcggca ggcaggcggt taccaactgg gcggcaggcg ttaccctgac
     2161 ctgggctttg ctgatgacgc tgttcctgcc gtggctggac gcggcgaaaa gccacgcgcc
     2221 ggtcgtccgg agtatggagg catcgctttc cccggaattg aaacgggagc tttcagacgg
     2281 catcgagtgt atcggcatag gcggcggcga cctgcacacg cggattgttt ggacgcagta
     2341 cggcacattg ccgcaccgcg tcggcgatgt acaatgccgc taccgcatcg tcctcctgcc
     2401 ccaaaatgcg gatgcgccgc aaggctggca gacggtttgg cagggtgcgc gtccgcgcaa
     2461 caaagacagt aagttcgcac tgatacggaa aatcggggaa aatatataaa aaacaacaga
     2521 ttgagccgaa tttctggatt aagtgccgga aatcgcgtat aattgcgcga ttaaaccttt
//
LOCUS       DAWISC010000199         2579 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWISC010000199 DAWISC010000000
VERSION     DAWISC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.199"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(195..266)
                     /locus_tag="WIW87_001913"
     tRNA            complement(195..266)
                     /locus_tag="WIW87_001913"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Arg,seq:cct)
     gene            complement(286..732)
                     /locus_tag="WIW87_001914"
     CDS             complement(286..732)
                     /locus_tag="WIW87_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ5175017.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAACILSFVVGKG"
     gene            complement(737..1711)
                     /gene="ispB"
                     /locus_tag="WIW87_001915"
     CDS             complement(737..1711)
                     /gene="ispB"
                     /locus_tag="WIW87_001915"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ5175018.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1727..1915)
                     /locus_tag="WIW87_001916"
     CDS             complement(1727..1915)
                     /locus_tag="WIW87_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175019.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1935..2243
                     /gene="rplU"
                     /locus_tag="WIW87_001917"
     CDS             1935..2243
                     /gene="rplU"
                     /locus_tag="WIW87_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ5175020.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2268..2540
                     /gene="rpmA"
                     /locus_tag="WIW87_001918"
     CDS             2268..2540
                     /gene="rpmA"
                     /locus_tag="WIW87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ5175021.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          757 a          692 c          574 g          556 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgaa gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgaaatcgt caaacctcgc caacaggaat cgaacctgta ttttacgctt aggaggcata
      241 cgttctatcc gttgaactat ggcgagccga aatgaaaagg agattttaac cctttccgac
      301 gacaaaagac aagatgcagg ccgcaatcag cggcccgaca gggataccgc ccataaatgc
      361 caccccgata accgtcccga ttaacagccc tgtaattaaa acaggctgct gtcccatcaa
      421 aggcacgccg cgtcccgcca gccaagccac gaaaataccg ataaaaacgg cggatatcat
      481 tttaaaattc aaaaattcgg caacgggagg aacctgcgcc tttcctgaaa ccaacggact
      541 caaaacccct atggtcaaaa gaatgatacc gagattcaac ccgtgcttct cgaccaacgg
      601 gacaaactgt atcaatgccg tctgctgcat cagcagcaat atggttgccg agatggtaat
      661 cgaattgttg ttgctgacca cccccaacag aatcagcgta accagaaaca ggggaacaaa
      721 actgaaattc atcgcctcaa gacaccctga ccaaagattc cttcgccagc tgaatcatgg
      781 catccttcac ttcgctgtcg ggcagggcat ccaaggcggt aacggcacaa tcgactgctt
      841 tgcgcgcctc gcctatcgaa tatgccaacg catccgaacg gacgacataa tcgtggattt
      901 tctcaaaata gctgcgatct gcattttcca aagcagtacg cacatcgttc gcaacctgtt
      961 cggaaccctg acgcatcaga taaatcaaag gcaaagtcgg ttttccttcc gccaaatcgt
     1021 cgccgacgtt tttgccggtt tcttcggttt cgccagaata gtccagcaca tcgtcaataa
     1081 tttggaatgc cgtaccgaca tacataccgt agtctttcaa cgcccgttcg tgttctgggg
     1141 aagccttgcc caaaattgcg ccgacttgag cggcagcttc aaacaatttt gccgttttat
     1201 attggatgac ttggatatat tgttcttcgg taatgtccgt attgccgatg ttcatcagct
     1261 gcatgacttc gccctcggca ataatgttgg ttgcatccgc catcacttcc aaaacgcgca
     1321 tactgcccga ggcaaccatc agttgaaagg cgcgcgtgta taaaaagtcg ccaaccaaca
     1381 cagccgccgc attgccgaac agattgtttg ccgttgcccg cccacggcgc aaatcgcttt
     1441 catcgacgac atcgtcgtgc aggagggtgg aagtgtggat aaactcgacc atcgccgcca
     1501 gcgaatacag tttctcgtca tcataaccga ccgccttacc cgccaaaatc gtcataatcg
     1561 gacgcaggcg tttgccgccc gcgctgatga tatatgtacc gatttgcgaa atcagtgcga
     1621 catcggattg caccgcacgg ttgatgactt cattgacttt ggcaaggtct tcaggcagat
     1681 gtcgctggaa atagggcaga ttctcgagca tggcagactc tcaggtttac gtttccgtca
     1741 aacggcagat accgcttgaa agctgattca aatagcgggc aattatatca gcaaagccat
     1801 cagggtacat cttttcagac ggcatatggt tatccgaacc gcgcaaactt tgacaaaaca
     1861 ggcaaataac aatacaatgc cgagttccgc acaatggtgt gcggatatta accataaccc
     1921 ttatggagtt gagtatgtac gcggtcgtaa aaaccggcgg caaacagtat aaagtttccg
     1981 tcggcgaaaa attgaaagta gaacagatac cagccgaact cgacagccaa atcgaactga
     2041 ccgaagtttt gatgattgct gacggcgaat ctgtaaaagt tggcgcaccc tttatcgaag
     2101 gtgcaaaagt aacggctaaa gtagtggcac acggtcgtgg cgaaaaagtc cgcatcttca
     2161 aaatgcgccg ccgcaaacac taccaaaaac gccaaggcca ccgccaaaat ttcacccaaa
     2221 tcgaaatcgt ggcaatcgcc taaccttaaa acttaggagc attacaaatg gcaagtaaaa
     2281 aagcaggcgg cagcacccgc aacggtcgcg attcagaagc caaacgcttg ggcgttaaag
     2341 cctacggcaa cgagctgatt cccgcaggtt ccatcatcgt acgccaacgc ggtaccaaat
     2401 tccacgcagg cgacaacgta ggtatgggca aagaccacac tttgttcgcc aaagttgacg
     2461 gttatgtcga attcaaaacc aaaggcgcgc tgaaccgtaa aactgtcagc atccgtcctt
     2521 acaccggttc tgaagaataa tccaaccgat acctgaagcc gcatcttttc acgatgcgg
//
LOCUS       DAWISC010000200         2565 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWISC010000200 DAWISC010000000
VERSION     DAWISC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.200"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(12..182)
                     /locus_tag="WIW87_001919"
     CDS             complement(12..182)
                     /locus_tag="WIW87_001919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175022.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            242..2467
                     /locus_tag="WIW87_001920"
     CDS             242..2467
                     /locus_tag="WIW87_001920"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ5175023.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          710 a          782 c          589 g          484 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttccccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc
      181 acgcacattc cccgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc
      241 tatgactcaa aaatccacca ttgtttatac ccataccgac gaagcacccg cgctggcgac
      301 ccaatcgctg ctgccgattg tgcaggcttt tgcccgccac gccgatattg atgtcaaaac
      361 cagcgacatt tctctctccg gccgtatttt ggcggcgttt cccgaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcgtc cgtaccgcaa ctgactgccg cgattaaaga
      541 attgcaatct aaaggctttg ccgttcccga ctatcccgcc gacccgcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgtc gcgcccctaa agcagtgaaa aactttgcga aaaaaaatcc
      721 gcacagcatg ggcgcatgga ccaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttt cataacgaac aatctgttat cgtacctgaa gcgacttccg tatccatcgt
      841 attcaccgac aaacaaggca acaaaaaaga gctgcgcgag cccgtcgccc tgaaagccgg
      901 cgaaatcatc gacgcgaccg tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtaaaagat gcgaaagcaa aaggcgtgtt gttctcgctg cacatgaaag ccactatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtattcttcg cgcctgtttt
     1081 tgaaaaattc ggcgacaaac tggctgctgc cggcgtcaac gttaacaacg gcttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc accgccgtcg aagccgaaat
     1201 cgccgccgtt tacgctgcca accccgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgatgcctct atgcctgcga tgattcgcaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcgcaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggccaagtcc aagtcattga
     1561 tgcggcagga aaagtcctaa tgcagcacga cgttgaagcc ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactt gccgtcaacc gcgcccgtct
     1681 gagcaacacg cctgccgtgt tctggctcga cgaaaaccgt ccgcacgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaat ggcctcgaca tccgcgtcct
     1801 cgctcctgaa gaagccgcca agttcagctt gggtcgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccaa ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca tgttcaacaa ttcctcgaag aaaaccattt
     2041 gcgctgggac tcgctgggcg aattcctcgc actcgccgta tcgtttgaac atttggcgca
     2101 aaaaaccggc aatgccaaag cccaagtcct cgccgacact ttggatgcag ccaccgaaaa
     2161 actgctgttg aacgacaaat cgcctaaacg taaagccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 actgaaagcc gcatttacgc cattggcagc cgctttgacc gccgacgaag cgaaaatcgt
     2341 tgccgagctt tccgccgtac aaggcaaagc ggccgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gccgcacaag tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 tccctatctg cctgattatg attaaacgcc gcacccattg gatat
//
LOCUS       DAWISC010000201         2560 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWISC010000201 DAWISC010000000
VERSION     DAWISC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.201"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..372
                     /locus_tag="WIW87_001921"
     CDS             <1..372
                     /locus_tag="WIW87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ5175024.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(590..1384)
                     /locus_tag="WIW87_001922"
     CDS             complement(590..1384)
                     /locus_tag="WIW87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ5175025.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1488..2213
                     /locus_tag="WIW87_001923"
     CDS             1488..2213
                     /locus_tag="WIW87_001923"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ5175026.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          683 a          798 c          571 g          508 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaaggattgt acccgaatta
      541 atattaagaa acgttaatcg cgaaaatata ttaacaaacc tgttgaaacc tattggtttt
      601 cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc
      661 gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcgccggcgg cataatccca cagcttctgc ccgccgtgaa
      781 cataaacatc ataacgcccg cacgccagat aacaccaatc caacgtactg ctgcccatac
      841 tccgtatcgt tccaaaaggc gcgagcgtac tcatacggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg aggcgcaggg
      961 gcagacgcgt cccgtttaaa aacgccccct gcccgcgttc ggcataaaaa cattcgccgc
     1021 tgactgggtt gtagattacg cccaactcgg cgcgcccgtt gcggacaaac gccaccgata
     1081 ccgcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1201 cctcacccaa catcggactg tcgattaaaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgagac ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcgcgc gaacgtataa tgtccgcttc
     1441 catcacgccg ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg ccccgattcc
     1501 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1561 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca
     1621 aggcacacgc cgcacggctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1681 acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa tccatctcct
     1741 ccggcgatcg catggatttc accctgcaaa aaagtgtcga actcggcgta accgccatac
     1801 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgatgg ggaacgcgcc gccaaacgcc
     1861 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     1921 cccccgtact gcccatcatc ggctaccgtg aagcactcga caaaatgccg tctgaaagca
     1981 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatacgc caaccgtccg
     2041 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2101 catttgaagc tggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2161 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2221 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2281 agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa actacagaga
     2341 tttatccgaa acaacaaccc tctccgccgt cattcccgca aaagcgggaa tctagaaacg
     2401 aaaaactaca gggatttatc cgaaacaaca aaccctctcc gccgtcattc ccgcgaaagc
     2461 gggaatctag aaatttaacg ttgcggtgat ttatcggaaa tgactgaaac tcaacggact
     2521 ggattcccgc ctgcgcggga atgacgaatt ttaggttgct
//
LOCUS       DAWISC010000202         2555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWISC010000202 DAWISC010000000
VERSION     DAWISC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.202"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(352..1986)
                     /gene="groL"
                     /locus_tag="WIW87_001924"
     CDS             complement(352..1986)
                     /gene="groL"
                     /locus_tag="WIW87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ5175027.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(2079..2369)
                     /gene="groES"
                     /locus_tag="WIW87_001925"
     CDS             complement(2079..2369)
                     /gene="groES"
                     /locus_tag="WIW87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ5175028.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          535 a          674 c          654 g          692 t
ORIGIN      
        1 acgaagctat ccgcacagaa acctgcacca cgtcattccc acgaaagtgg gaatccagaa
       61 cgtaaaatct gaagaaaccg ttttatccga tacgtttccg caccgacaga cctagattcc
      121 cgctttcgcg ggaatgacgg cggaaaggtt gctgtttttc cgataaattc ctgccgctct
      181 tcgtttttgg gatggcggga aataaaacaa aagcgcgcgt atcaaaaaac aaaaatgcaa
      241 agaacggcgt tggcaatttt aaggtttgcg ccttagccgc acttattcgc aaaaaaaacc
      301 gcacggcgtt gaccgtgcgg ttttttatct gaaagcttca gacggcattg cttacatcat
      361 gccgcccata ccacccatgc cgcccatatc aggcacagcc ggtttgtctt cggggatttc
      421 agcgatcatg caatcagtgg tcagcatcaa gccggcgata gatgcggcgt gttgcagcgc
      481 agaacgggtt actttggcgg ggtcgagtac gcccatttcg atcatatcgc cgtattcgcc
      541 gctgccagcg ttgtaaccgt agttgccttt gccttccaat actttgttca caaccacgct
      601 gggttcgccg cctgcgttgg caacgatttg gcgcagcgga gactcaacgg cgcgcaagac
      661 gatttgtacg cctgcgtctt ggtcggcatt gccggtgtgc aggttttcca aagcagcacg
      721 ggcacgcaac agggctacgc cgccgcctgc aaccacgcct tcttcaacgg ctgcgcgggt
      781 agcgtgcagc gcgtcttcca cgcggtcttt tttctctttc atttcgactt cggtcgcggc
      841 accgactttg atgactgcca cgccgcctgc caatttagcc acgcgctctt gcagtttttc
      901 tttgtcgtaa tcgctggttg cggtttcgat ttgttggcgg atttcggcaa cacgcgcttc
      961 gatttgggct gcgtcgccaa agccgtcgat gatggtggtg ttttctttac cgatttcgat
     1021 gcgtttggct tgacccaagt cgtccaaagt cgctttttcc aaagacagac cgacttcttc
     1081 ggaaatcacc acgccgccgg tcaggatggc gatgtcttgc aacatcgctt tgcggcggtc
     1141 gccgaagcca ggggctttga cggcaacggt tttcaggatg cctcggatgt tgttcacgac
     1201 caaagtcgcc aaggcttcgc cttctacgtc ttcagcgata atcaacagcg gacggctggc
     1261 ttttgccact tgttccaaaa caggcagcag gtcgcggatg ttgctgattt ttttgtcgaa
     1321 caacaataca aacggattgt ccaaagcagc gatttgtttt tccgcatcgt tgatgaagta
     1381 aggagacagg tagccgcggt cgaactgcat accttcaact acgtccagct cgttttccaa
     1441 agacttgccg tcttcaacgg taatcacgcc ttctttgccg actttttcca tcgcttcggc
     1501 gataatcgcg ccgacttgtt cgtcggagtt ggcggaaata gagccgactt gggcgatttc
     1561 tttagaagtg tcgcaaggtt tggcgatgtt tttcagttcg tcaaccaaag cggcgacggc
     1621 tttatcgata ccgcgtttca ggtcggtcgg attcatacct gcggtaacat atttcatacc
     1681 ttcggcaacg atggattgcg ccagtacggt ggcggtagtc gtaccgtcgc ctgccacgtc
     1741 gttggttttg gacgcaactt ctttcaccat ttgcgcgccc atattttcaa acttgtcttt
     1801 cagttcgatt tctttggcga cggttacgcc gtctttggtg atgtgcgggc cgccgaatgc
     1861 gcggtcaacg actacgttgc gacctttggg gcccaaggtt acgcggacgg cgtttgccag
     1921 aatgttcacg ccgtttacca ttttttgacg gacttcattg ccgaactgta cgtcttttgc
     1981 tgccatttca attctccaaa aatcattaaa actgtctgat aaaaccgttt atgccgtctg
     2041 aaggcggttt gccgtttcag acggcatcgt gtccgtattt atttttcaac gatgccgaaa
     2101 atatcttctt cgcgcattac caacagctct tcgccgtcgg cttttacggt ttggccgctg
     2161 tatttgccga agatgatttt gtcgccgact ttgacatcca gcggacggcg gctgccgtct
     2221 ttaccgattt tgcccgcgcc cacggcgatg acttcgccca tatcgggttt ttcggcggcc
     2281 gcacccggca aaacgatgcc cgatgcggtt ttttcttcag cttccaagcg tttgacgaca
     2341 acgcggtcgt gtaaaggacg gatggtcata tttatgctcc gataaaatag tttgaaaaca
     2401 atcatctgcc cgaacggttc aggcagattg aagtggaaac cggacagccg tcaagcagct
     2461 gcccgtataa gtcggcaaaa ttagggtgtg tgcgggtaaa ttcaagtgag gcggaaaaaa
     2521 tttatttccg ccgtttttta tagtggacta aattt
//
LOCUS       DAWISC010000203         2502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWISC010000203 DAWISC010000000
VERSION     DAWISC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.203"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="WIW87_001926"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="WIW87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ5175029.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2202
                     /locus_tag="WIW87_001927"
     CDS             1063..2202
                     /locus_tag="WIW87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEZ5175030.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            2335..>2502
                     /locus_tag="WIW87_001928"
     CDS             2335..>2502
                     /locus_tag="WIW87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5175031.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIESILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT          683 a          724 c          620 g          475 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg ccgccttttc cggtgggcaa aaaatcagcc
       61 ctcggaaaac gcggtttgca aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
      121 gctgcgccga tactttacgc cacactcatc ccgacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc gccgtcgctt ccgcacaggc
      241 gggcgcggta gacgcgctta agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgaccgggc cttttcaaat gggaatacac caaaccttac aggcaaacca tcgtcggcga
      421 cggtcaaacc gtttggctct acgatgttga tctggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaac gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 ttttacgcag gcggaacaat gtcccgcatt accgcccgat cgggcaccgg aacggcaaac
      901 cggtgaaaat taacggttgc gccccggctg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccgtccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcggcaa tcctgagcct cgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     1141 caaaccccct ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg
     1201 tcggattatg tcgatcccga aaccgttgcc gcctttgaaa aagaaaccgg catcaagacg
     1261 cgttccgatt attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     1321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     1381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     1441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     1501 aataccttgg caatcaatac ccagcaggtg aaaaaagcat tgggtacgga caagctgccc
     1561 gaaaacgaat gggatttggt gttcaaaccc gaatacaccg ccaaactcaa atcctgcggc
     1621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     1681 gaccccaaca gtgagaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     1741 cggggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     1801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     1861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtggg cgtgtgggtg
     1921 gattccttta tgattccgcg cgacgcgcaa aacgttgcca atgcccaccg ctatatcgac
     1981 tacacgctcc ggcccgaggt ggcggcgaaa aacggcagct tcgttaccta cgcgcccgcc
     2041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     2101 aacaaagaac tgatggaaaa aagtttcatc gtatcgccca aatccgcaga atccgtcaaa
     2161 ctgggcgtga agctgtggca agggctcaaa gcgggcaaat aaccggaatc cctgccgtct
     2221 gaaacctttc gggcggcagg aaacggcgcg tccgttatca aacagggggg cgtttcccct
     2281 cctgccggtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcctat ttataatgtc gaaagttatt tggaagcgtg tttaagttcc
     2401 atagaatcta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttacaaatat atagataaaa ta
//
LOCUS       DAWISC010000204         2477 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWISC010000204 DAWISC010000000
VERSION     DAWISC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.204"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(148..735)
                     /locus_tag="WIW87_001929"
     CDS             complement(148..735)
                     /locus_tag="WIW87_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ5175032.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            899..2305
                     /gene="dnaB"
                     /locus_tag="WIW87_001930"
     CDS             899..2305
                     /gene="dnaB"
                     /locus_tag="WIW87_001930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ5175033.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGRLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          634 a          632 c          650 g          561 t
ORIGIN      
        1 cgaaacctga cagtaaccgt agcaactgaa ccgtcattcc cacgaaagtg ggaatctaga
       61 aataaaaagc aacaggcatt tatcggaaat aactgaaatt caataccgca aaaatctacc
      121 cgaaatgata tagcggatta acaaaaatca ggacaaggcg gcaaaacgtt tggcgacttc
      181 gtcccagttg acgatttccc aaaaaccttt caggtagttg ggacggctgt tgcggtagtc
      241 gatgtaatag gcgtgttccc acacgtcgca ggtcagcagc ggcgtgtttt cagtggtcag
      301 cggcgtagcg gcgttggaag tagaaaccaa atccaatccg ccggcagggg tttttaccag
      361 ccacgcccaa ccggagccga aagtaccggc cgcgcaggca ttgaacgctt cttggaattt
      421 ctcgaagctg ccccatttcg cgtcgatggc ggcggccagt tcgccggcag gtttgccttg
      481 acctttggac gtgaaaccca gccagtagaa ggtgtggttc caagtttgtg ccgcgttgtt
      541 gaacacgccg cctgaagatt ttttcacaat ctcttccaaa ggcaggtttt caaattcggt
      601 gcctttgatt tgattgttca ggttggtgat gtaggtttga tggtgtttgc cgtagtggaa
      661 ctccaaagtc tctttgctca gatgcgggga caatgcgtcc agttcataag gcagttgcgg
      721 cagcttatgt tccattttgt actcctgaat attgttttaa atgttgtatt ttggcagtgt
      781 tgctgcaaat aactcggcag cccgtgtatt ctacctgttt tgcggtgcgg aaaccaatta
      841 aacctgcttt acgctataat agaagattgc aatttcggca cgacagatag gatgtaccat
      901 gaacgattac gcagccatgc cgtctgaaga ccgtgaggtc ggcgcattgt ctctgccccc
      961 acactcaatg gaggcggaac aatccgtttt gggtgggttg atgctggaaa atccggcatg
     1021 ggacaggatt gccgatgtgg tttccggtga agacttctac cggcatgaac accgcctgat
     1081 tttccgatcc attgccaaat tgattaatga gagccgtccc gccgatgtga ttacggttca
     1141 ggaagatttg cagcggaacg aagaattgga agcggcaggc ggattcgaat atctgattac
     1201 gctggcgcaa aacaccccgt ctgccgccaa catccgccgc tacgccgaaa tcgtgcgcga
     1261 gcgttccatt atgcgccaac tcgccgaagt ggggacggaa atcgcccgca gcgcatacaa
     1321 tccgcaaggc agggacgcgg ggcagctttt ggacgaggcg gaaaacaaag tattccaaat
     1381 cgccgaaagc accgccaaat ccaagcaggg ctttttggag atgcccgatt tgctgaaaga
     1441 agtcgtacag cgcatcgata tgctctactc gcgcgacaat cccgatgaag ttaccggcgt
     1501 gccgacgggg ttcatcgacc tcgacaaaaa aacctcgggt ctgcaacccg gcgacctgat
     1561 tatcgttgcc ggtcgtccgt ctatgggtaa gaccgccttt tctatcaata tcgccgaaca
     1621 cgttgccgta gaaggcaggc tgcccgttgc tgttttctcg atggaaatgg gcggggcgca
     1681 actggtcatg cgtatgctcg gctcggtcgg acggttggat caaagcgttt tgaaaaccgg
     1741 caggctcgaa gacgaacact ggggtcgcct gaacgaagca gtcgtcaaac tctccgacgc
     1801 gcccgtgtac atcgacgaga ccccgggtct gaccgcgctc gaactgcgcg cccgtgcccg
     1861 ccgtctcgcc cgtcaattta acaataagct tggattgatt gtcatcgact acctgcaact
     1921 gatggcagga tccggccgtt ccgacaaccg agcttcggag ctgggagaga tttcacgttc
     1981 gctcaaagcg ttggcgaaag aattgcaagt ccccatcatc gccctgtcgc aattgagccg
     2041 cacggtcgaa tcgcgtaccg acaaacgccc catgatgtcc gaccttcgcg agtccggcgc
     2101 aatcgagcag gatgccgacc tgattatgtt catgtaccgc gacgaatact acaaccagga
     2161 ctcacccatg aaaggccttg ccgaatgtat catcggcaaa caccgcaacg gtcccgtcgg
     2221 taaaatcttc ctcacatgga cgggacaatt caccaaattc gacaatgctg cctatattcc
     2281 cgaggaggca aagatagagg attaaatggc tatataaaaa tttattaggc gaaatcaggc
     2341 aaaatcgttt aaaatcatgc tgagagattg ccctaaaaaa taaaacgcgg tcttgaggca
     2401 tttttgcatt cagcccgcat ataattgaaa atatagtgga ttaacaaaaa tcaggacaag
     2461 gcaacgaagc cgcagac
//
LOCUS       DAWISC010000205         2468 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWISC010000205 DAWISC010000000
VERSION     DAWISC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.205"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(13..819)
                     /locus_tag="WIW87_001931"
     CDS             complement(13..819)
                     /locus_tag="WIW87_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ5175034.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGCGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WIW87_001932"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WIW87_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ5175035.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLAGRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          683 a          619 c          642 g          524 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccact
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcaccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc acccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtatgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccattt cggtcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt catcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagta gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc aggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgttg aaaaacgatc agaagaaaaa ggttttggtg
     1441 gtatccgccg acgtttaccg tcctgccgcg attgaacagc tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg attttttccc gtccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccac gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cggccgcatt
     1921 ttgggtatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat agacgaagaa
     1981 gccgccgcta aaatggcgaa aaagctgcac aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat ctcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaaatt tcagacggcg tttttgtttt
     2461 atattctg
//
LOCUS       DAWISC010000206         2461 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWISC010000206 DAWISC010000000
VERSION     DAWISC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.206"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..1032
                     /gene="rfbB"
                     /locus_tag="WIW87_001933"
     CDS             <1..1032
                     /gene="rfbB"
                     /locus_tag="WIW87_001933"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ5175036.1"
                     /translation="IGSAVVRHIIRNTRDAVVNVDKLTYAGNLESLTEVADNPRYAFE
                     QVDICDRAELDRVFAQYRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARA
                     YWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVR
                     AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLI
                     TFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNGSY
                     RLERLGTGK"
     gene            1095..1961
                     /gene="rfbA"
                     /locus_tag="WIW87_001934"
     CDS             1095..1961
                     /gene="rfbA"
                     /locus_tag="WIW87_001934"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ5175037.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2015..>2461
                     /gene="rfbC"
                     /locus_tag="WIW87_001935"
     CDS             2015..>2461
                     /gene="rfbC"
                     /locus_tag="WIW87_001935"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5175038.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYKPEAEQVLIWNDP"
BASE COUNT          622 a          683 c          609 g          547 t
ORIGIN      
        1 atcggttctg ccgttgtccg tcatattatc cgaaacaccc gggacgctgt cgtcaatgtc
       61 gataagctga cttatgccgg caatttggaa tctttgactg aggtagccga taatcctcgc
      121 tatgcttttg aacaagtgga tatttgcgac cgcgccgaac tcgaccgcgt attcgcgcaa
      181 taccggcctg atgccgtgat gcacttggcg gcggaaagcc atgtcgaccg ctctatcggt
      241 tcggcaggcg agtttatcca aaccaatatc gtcggcacat tcaatctgct tgaagcagcc
      301 cgcgcctact ggcaacaaat gccgtctgaa cagcacgaag ccttccgttt ccaccatatt
      361 tccaccgatg aagtctatgg cgatttaggc ggcacggacg atttgtttac cgaaaccgcg
      421 ccctacgcgc cgtccagccc ctactctgcc tctaaagcgt ccagcgacca cctcgtccgc
      481 gcgtggttgc gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt
      541 ccttaccatt ttccggaaaa actcattcct ttgatgattc tgaacgcgct tgacggcaaa
      601 ccgctgcctg tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac
      661 gcgcgcgcac tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc
      721 ggccacaatg aaaaagccaa tattgaagtc gtcaaaacca tctgcgccct gctggaagaa
      781 ctcgctcccg aaaaaccggc cggtgtggcg cgttatgaag atttgattac tttcgtacaa
      841 gaccgccccg gccatgacgt acgctacgcc gtcgacgcag ccaaaatcag gcgggatttg
      901 ggctggctgc ctttggaaac cttcgagtcc ggcctccgca aaaccgtgca atggtatctg
      961 gacaacaaaa cctggtggca aaatgtattg aacggcagct atcgtttgga acgtttaggt
     1021 actggaaaat agttttcaga cggcatcccg acgcaatgcc gtctgaaaac ccatcgcaaa
     1081 ggaagaaaga aaagatgaaa ggcatcatac tggcaggcgg cagcggcacg cgcctctacc
     1141 ccatcacgcg cggcgtatcc aaacagctcc tgcccgtgta cgacaaaccg atgatttatt
     1201 accccttgtc ggttttgatg ctggcgggaa tccgcgatat tttggtgatt accgcgcctg
     1261 aagacaacgc ctctttcaaa cgcctgcttg gcgacggcag cgatttcggc atttccatca
     1321 gttatgccgt gcaacccagt ccggacggct tggcacaggc atttatcatc ggcgaagaat
     1381 ttatcggcaa cgacaatgtt tgcttggttt tgggcgacaa tattttttac ggtcagtcgt
     1441 ttacgcaaac attgaaacag gcggcagcgc aaacgcacgg cgcaaccgtg tttgcttatc
     1501 aggtcaaaaa ccccgaacgt ttcggcgtgg ttgaatttaa cgaaaacttc cgcgccgttt
     1561 ccatcgaaga aaaaccgcaa cggcccaaat ccgattgggc ggtaaccggc ttgtatttct
     1621 acgacaaccg cgccgtcgag ttcgccaaac agctcaaacc gtccgcacgc ggcgaattgg
     1681 aaattaccga cctcaaccgg atgtatttgg aagacggctc gctctccgtt caaatattgg
     1741 gacgcggttt cgcgtggctg gacaccggca cccacgagag cctgcacgaa gccgcttcat
     1801 tcgtccaaac cgtgcaaaat atccaaaacc tgcacatcgc ctgcctcgaa gaaatcgctt
     1861 ggcgcaacgg ttggctttcc gatgaaaaac tggaagaatt ggcgcgcccg atggcgaaaa
     1921 accaatacgg ccaatatttg ctgcgcctgt tgaaaaaata atgtttgagg ccgtctgaaa
     1981 cttttcagac ggcctttaga tgaaagataa aaagatgaac atcattgata ccgccattcc
     2041 tgacgtaaaa ctgcttgagc cccaagtctt cggcgacgcg cgcggctttt ttatggaaac
     2101 cttccgcgac gagtggttta aaacccaagt ctgcgaacgc accttcgtgc aggaaaacca
     2161 ctccaaatcc ggcaaaggcg tattgcgcgg cctgcactat caaactgaaa acacacaagg
     2221 caaactcgta cgcgtggttg tcggcgaagt attcgacgtg gccgtcgata tgcgtaaaga
     2281 ctcccccact ttcggcaaat gggtaggcga aattctgtcc gcagaaaaca aacgccaact
     2341 gtgggtaccc gaaggtttcg cacacggctt ctatgtactg agcgatgaag ccgagttcgt
     2401 ctataaatgc acggactatt acaagcctga agccgaacag gttttaatat ggaacgaccc
     2461 g
//
LOCUS       DAWISC010000207         2454 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWISC010000207 DAWISC010000000
VERSION     DAWISC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.207"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(119..1570)
                     /locus_tag="WIW87_001936"
     CDS             complement(119..1570)
                     /locus_tag="WIW87_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ5175039.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1593..2213)
                     /locus_tag="WIW87_001937"
     CDS             complement(1593..2213)
                     /locus_tag="WIW87_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ5175040.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          767 a          581 c          574 g          532 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaata acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacgatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcagtccaa cgacgaaaat gcggcgtatg ccagacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     1081 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     1141 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     1201 cggatttgtt gccatccgta cccacatggt gtacgagcgg gaaaatcaac cctctgacga
     1261 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     1321 accacgcagc acccaaaatc aaaaagtcgg caatacccgc ccacaagaag cactgcccga
     1381 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgacgg
     1441 cgataccggt cagaaaacag gcaagggtaa atgaaaatgc ctgaggcacg gactgtgctg
     1501 cgaacgaaaa catcctcacc cacaagactt ctatacccaa gctcaataag ccgctaagga
     1561 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt cactatatcc atcctttctg
     1621 taacgcagcc atactgaagc agcaatcaga aggttaaagc aggctgtcag cgcaatggtt
     1681 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     1741 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     1801 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     1861 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     1921 ccggaaatca gacccctgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     1981 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccaccgaa atacgcacct
     2041 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaactcaa atcgatacct
     2101 atgtgactga atagaagcct ctgccagctg acctggtaaa tcagggcgca gaatccagag
     2161 gcgaaaaaca ccagcgacaa tcctttcagg gttttacttg cggtcgaatt catattttct
     2221 attttcaaat aggcggaact ataaatgaaa tgtatcaatg tgtaaacatt tgtattaacc
     2281 gtaggaaatc accgtattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     2341 acggcgtatc actgttcaaa ctgctgtgcg gcttcacggt gttataaaaa taatggggct
     2401 gtcctggaca actaagataa actcgatttt actaattgtt ttaaaatgga aatt
//
LOCUS       DAWISC010000208         2432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWISC010000208 DAWISC010000000
VERSION     DAWISC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.208"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            35..1390
                     /locus_tag="WIW87_001938"
     CDS             35..1390
                     /locus_tag="WIW87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ5175041.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WIW87_001939"
     CDS             1394..2404
                     /locus_tag="WIW87_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ5175042.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHMPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRCRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          443 a          656 c          738 g          595 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttagacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggtaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggct tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggtctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgat gatgcggctt cctatgcgga caatccgttt cagattaaca aacagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatg ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgctgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gc
//
LOCUS       DAWISC010000209         2343 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWISC010000209 DAWISC010000000
VERSION     DAWISC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.209"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            44..2257
                     /locus_tag="WIW87_001940"
     CDS             44..2257
                     /locus_tag="WIW87_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ5175043.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPSDAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          596 a          759 c          568 g          420 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc tccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgattg gaacctattg gtttccgaac gctgcaacag
      361 taccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacgc cagaagaacg cgcccagcaa gcggaaacca tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga gcccgaaaaa taccgcgaaa tcgcgctgct
      721 tttggacgaa aaacgcaccg aacgcctcga atacatcgaa aacttcctca acatcctgcg
      781 cggtgaactc aagaaataca atgtccattt cgaagtcgcc ggccgcccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacggcctct ttgacatccg
      901 cgccgtgcgg attctggttg ataccgtccc cgagtgttac accacgctgg gcattgtcca
      961 cagcctttgg cagcctatcc ccggcgagtt cgacgactac atcgccaacc cgaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggtcccgaa gacaaaggtg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgcgc actggcgtta
     1141 caaagaaggc ggcaaaggcg attccgccta cgagcaaaaa atcgcctggt tgcgccaact
     1201 tttggactgg cgcgaaaata tggcggaaag cggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctg ttcaacgaca cgatttacgt tttaaccccg cacggcaaag tcctttccct
     1321 gcctacgggc gcgacaccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ctgccggggc gcgaaagtcg aagggcaaat cgtgccgctg tccaccccgc ttgaaaacgg
     1441 acagcgcgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctgtacga
     1501 aggctgggtc aaatccggca aggcaatcag taaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaaatcgac aagcagcttg ccaaactcac
     1621 gcccaagccc aacctgcaag agcttgccga aaacctcggc ttcaaaaaac tcgacgacct
     1681 ctacactgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa cctccgcccg tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gtgtgctcat tgatggcgaa gacggtttga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacaagcacc
     1981 tgaaaaagtg ctggacgcaa gctgggcggc gttgcaggaa ggacaagtgt ttgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcgctcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagtat
     2161 gcggttcacg ctcgaagtca aacaagttac cgacctccca cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgtataacgc ttcagacggc attttgattg ccggggtttg ctattttttg ttgcataatc
     2341 aat
//
LOCUS       DAWISC010000210         2308 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWISC010000210 DAWISC010000000
VERSION     DAWISC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.210"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIW87_001941"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIW87_001941"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ5175044.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLDAQKQLREQGILNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WIW87_001942"
     CDS             1627..2193
                     /locus_tag="WIW87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ5175045.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          526 a          560 c          662 g          560 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tctggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagttc
      301 cgtctgtgcc ggcgatgggt agcgtgtcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacaggcccg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga tacggcaagt tcgcgccgga
      481 cggcagacgc cgcctgttcg gaaacggcgg gcagtttgcc gtcgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttctttca
      601 tcggtttggc gtgtgcaacg gcaagtgtct gcgcgccttc cggcgtgtcg gctatgccga
      661 taccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgccgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgag tatgccttgt tcgcgcaact gtttttgagc atcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat ataggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtt
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctacccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggcaggcat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcc caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat tgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtg
//
LOCUS       DAWISC010000211         2302 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWISC010000211 DAWISC010000000
VERSION     DAWISC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.211"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            19..747
                     /gene="rpe"
                     /locus_tag="WIW87_001943"
     CDS             19..747
                     /gene="rpe"
                     /locus_tag="WIW87_001943"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ5175046.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WIW87_001944"
     CDS             complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WIW87_001944"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ5175047.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1883..2191)
                     /locus_tag="WIW87_001945"
     CDS             complement(1883..2191)
                     /locus_tag="WIW87_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175048.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          461 a          546 c          741 g          554 t
ORIGIN      
        1 gatattgaag gaaaaaacat gactacttac cgtatcgcac ccagcatttt atcggcagac
       61 tttgcccgcc tcggagaaga ggtggaaagc gtcattgcgg caggtgcaga tctgatccat
      121 tttgacgtga tggacaacca ttatgtgccg aacctgacct tcggccctat ggtttgcgcg
      181 gcgttgaagc cttatgcaag cgtgccgatt gatgtgcatc tgatggtcga acccgttgac
      241 gacctgattc agtcgtttgc caaagcagga gcatcaatca tcacgttcca tcccgaggcg
      301 agccgccata tcgaccgcag cttgagcctg attcgtgata tgggctgtca ggcggggctg
      361 gtgttgaatc cggcaacgcc cgtatatctg ttggaaaacg tattggacag gctggatatg
      421 gttttgctga tgtcggtcaa ccccggattc ggcggacaaa gcttcatccc gcacaccctt
      481 gaaaaaatcc gccgggtgcg ggcgatgctg gatcggtacg aagcacaaag cgggcggcac
      541 atcgccatcg aagtggacgg cggcatcaaa accgacaata ttgccgccgt tgcccgagct
      601 ggtgcggata cctttgttgc cggttctgcg attttcggca aaccagacta taaggcggtg
      661 attgatgcga tgagggcgga actggaaaaa agcggcaggc tgaaccttgc gccaatgatt
      721 gaaatagaaa tgccgtctga aaattagctc tgtttcagac ggcattgcgt tatttttcga
      781 cgggcagccc gaaatgcagg taggcgcgtt cggtcgccat cctgccgcgc ggggtgcgtt
      841 gcaggaagcc ttgttggata aggtagggtt cgataacgtc ttcgatggtg tctgtagatt
      901 cgccgatggc ggcggcaaca ttgtccaaac cgaccgggcc gccgccgaat ttgtgcaaaa
      961 cggcttcgag aaatttcctg tccatcacgt ccagcccctg cgcgtccacg tccagcatac
     1021 ttaaagcggc atcggcgatg ccgccgtcga ttgtgccgtt gtttttcacg tcggcgaaat
     1081 cgcgcacgcg tcgcaacagg cggttggcga tgcgcggcgt accgcggctg cgtttggcga
     1141 tttcttccgc gccttcttcg gacatatcga gctgcaacag ttgtgccgaa cggctgacga
     1201 tggtggtaag gtctcggttt tcgtaaaact caaggcggga gacgatgccg aagcggtcgc
     1261 gcaacggatt ggtcagcata ccggcgcggg tggtcgcgcc gatgagcgtg aagggcggca
     1321 ggtcgatttt gacggaacgg gcggcgggtc cttcgcctat cataatgtcg agccggtagt
     1381 cttcgagcgc gggatagagg atttcttcga caacagggct gaggcggtgg atttcgtcga
     1441 tgaacaatac atcgtgcgga tcaaggttgg tcaaaagggc ggcgaggtcg cctgcgcgtt
     1501 cgaggacggg gccgctggtt tggcgcaaat ttacgcccaa ttctttggcg atgatgtgcg
     1561 ccagtgtggt tttgcccagt ccgggcgggc cgaagagcaa aacgtggtcg agtgcttcgc
     1621 cgcgtttttt ggcggcttgg atgaaaatgg caagctgttc tttggctttg tcttgcccga
     1681 tgtagtcgtc cagcgttttg gggcggaggg cgcgttcgag cagttcttcc tgtgcggagg
     1741 cggtttgggc ggcaacgatg cgttgcggtt gcgcggcggt caggttgtcg gtttgcagca
     1801 tagtgttccc tttgtcgggt atgccgtccg aacggtcggc ggcgtttcag acggcattga
     1861 aaagataacg gtcaaaagcg ttttatccgc gggtttcgcg ccaaacgaga tagtcgcgca
     1921 ggggcggcat aggcgggttg atgcagtggg ggcagatgcc ggtggtgtct tcgtcgtcgc
     1981 tgtcgatgtg gtaggcgttg gggtcgaagc ggatttgctg catgacggct tgcgccaatg
     2041 cctgcgcctg aacaacgtcg aaattgaatt tttcgaggat ttcgttcaac aggcggattt
     2101 cgccaccgct aaattccgca ctgtgtttga ggatgaggcg ttgataatct tcgttgttga
     2161 ttttaggcaa taaatcggtt ttcagggtca tgattttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaactttat agtggattaa ca
//
LOCUS       DAWISC010000212         2226 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWISC010000212 DAWISC010000000
VERSION     DAWISC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.212"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..2064
                     /locus_tag="WIW87_001946"
     CDS             <1..2064
                     /locus_tag="WIW87_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ5175049.1"
                     /translation="WFGPVQNGKVQMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPGR
                     YSLADLSENEIKSKEPSFTSRQTVIRLDKGVHQIKLQGNEVANFNGNDGKNDTFGIVS
                     EGSFMPDASEWKKVLLPWTVRASNDDGQFNTFNKEEKDGKPKYSQKYRSRDNGKHERN
                     LGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRKDIQNTE
                     STLAKELRAFAEKSYVGDRYGVDGGFVLRKVERNGKDHVFMFGAMGFGGRGAYALDLS
                     KIDSGNGNLADVSLFDVKHDKNGNNGVKLGYTVGTPQIGKTHDGKYAAFLASGYATKD
                     ITSGDNKTALYVYDLESSGTLIKKIEVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGG
                     SMYRFDLSNQDPNQWSVRAIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVLST
                     SEQYIYGIFDDDTVANNVNVKLSGLGGGLLEQELKQEDKTLFLTDYKRSDGSGSKGWV
                     VKLKGGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADGGKLTKKSARPI
                     VPAENQAVAQYSGHKKGINGKSIPIGCMQKGNEIVCPNGYVYDKPVNVRYLDEKKTDG
                     FSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISW
                     REVFY"
BASE COUNT          624 a          549 c          594 g          459 t
ORIGIN      
        1 tggttcggac ctgtgcaaaa tggcaaggtg cagatgtatt ccgcttcggt ttccacctac
       61 cccgacagtt cgagcagccg cattttcctt caaaatctga aaagaaaaaa cgaccccaac
      121 aaacccggcc gctattccct cgcagacttg agtgagaatg agattaaaag taaagagccg
      181 agtttcacaa gtcggcaaac cgtcatccga ttggataaag gcgtacatca gatcaaactt
      241 caaggcaatg aggtcgccaa ttttaatgga aatgacggca aaaacgacac tttcggcatt
      301 gttagtgaag ggagcttcat gcctgatgcc agcgagtgga aaaaagtatt gctgccttgg
      361 acggttcgtg cttccaatga tgacggtcaa tttaacacat tcaacaaaga agaaaaagac
      421 ggcaagccaa aatacagcca aaaataccgc agccgcgaca acggcaagca cgagcgcaat
      481 ttgggcgaca tcgtcaacag ccccatcgtg gcggtcggcg agtatttggc tacttccgcc
      541 aacgacggga tggtgcatat cttcaaacaa agcggcgggg acaagcgcag ctacaatctg
      601 aagctcagtt atatcccggg tacgatgccg cgcaaggata ttcaaaacac cgaatccacc
      661 cttgccaaag agctgcgcgc ctttgccgaa aaaagctatg tgggcgaccg ctacggcgtg
      721 gacggcggct ttgtcttgcg caaagtcgaa cggaacggga aagaccatgt gtttatgttc
      781 ggcgcgatgg gctttggcgg cagaggcgcg tatgccttgg atttaagcaa aatcgacagc
      841 ggcaacggca acctggcaga cgtttccctg tttgatgtca aacatgacaa gaatggcaat
      901 aacggcgtga aattaggcta caccgtcggc acgccgcaaa tcggcaaaac ccacgacggc
      961 aaatacgccg ctttcctcgc ctccggttat gcgactaaag acattaccag cggcgacaat
     1021 aaaaccgcgc tgtatgtgta tgatttggaa agcagcggca cgctgattaa aaaaatcgaa
     1081 gtacccggtg gcaagggcgg gctttcgtcc cccacgctgg tggataaaga tttggacggc
     1141 acggtcgata tcgcctatgc cggcgatcgc ggcggcagta tgtaccgctt tgatttgagc
     1201 aatcaagatc ctaatcaatg gtctgtacgc gccatttttg aaggcacaaa accgattact
     1261 tccgcgcccg ctatttccca actgaaagac aaacgcgtgg ttatcttcgg cacgggcagt
     1321 gatttgagtg aggatgatgt actcagtacg agcgaacaat atatttacgg tatcttcgac
     1381 gacgatacgg tggcgaataa cgtaaatgta aaactcagcg gtttgggagg cgggctgctc
     1441 gagcaagagc ttaagcagga ggataaaacc ttattcctga ccgattacaa gcgatccgac
     1501 ggatcgggca gcaaagggtg ggtagtgaaa ttgaagggcg gacagcgcgt taccgtcaaa
     1561 ccgaccgtgg tattgcgtac cgcctttgta accatccata aatatacggg tacggacaaa
     1621 tgcggcgcgg aaaccgccat tttgggtatc aataccgccg acggcggcaa gctgaccaag
     1681 aaaagcgcgc gcccgattgt gccggccgag aatcaggctg tcgcgcaata ttccggccat
     1741 aagaaaggca tcaacggcaa atccatccct ataggttgta tgcaaaaagg caatgaaatc
     1801 gtctgcccga acggatatgt ttacgacaaa ccggttaatg tgcgttatct ggatgaaaag
     1861 aaaacagacg gattttcaac aacggcagac ggcgatgcgg gcggcagcgg tatagacccc
     1921 gccggcaagc gttccggcaa aaacaaccgc tgcttctccc aaaaaggggt gcgcaccctg
     1981 ctgatgaacg atttggacag cttggacatt accggcccga cgtgcggtat gaaacgaatc
     2041 agctggcgtg aagtcttcta ctgatttgca cgcgaaaatg ccgtccgaaa ggttttcgga
     2101 cggcattttt tgcgtttttc gggaggggcg ggttcgtaaa aggcgggcta tagggtaggc
     2161 ttcatctcgc caatctcact gaatccatca atttccacaa ttcaattaaa taccgtcaaa
     2221 ccgatg
//
LOCUS       DAWISC010000213         2207 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWISC010000213 DAWISC010000000
VERSION     DAWISC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.213"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            32..516
                     /locus_tag="WIW87_001947"
                     /pseudo
     CDS             32..516
                     /locus_tag="WIW87_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            612..1235
                     /gene="rsmG"
                     /locus_tag="WIW87_001948"
     CDS             612..1235
                     /gene="rsmG"
                     /locus_tag="WIW87_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ5175050.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1334..2107
                     /locus_tag="WIW87_001949"
     CDS             1334..2107
                     /locus_tag="WIW87_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ5175051.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          432 a          505 c          768 g          502 t
ORIGIN      
        1 atgcggtctg cttcggaaga gggggacggc gatgtttgtg aacgagaaat atccttatgc
       61 ggctctgttt gcgggactgg tgtttttgac gctgccgttt gcgttggcgg tgcatgatgc
      121 ctttgcgctt gcgttcggac ggacggggtt gctggtgtcg gtgtcggacg gcggattcgg
      181 ctggcgtggc ggttgggacg gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa
      241 tgtggttgtg tcggcgggtc tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc
      301 gcgttacaca ctgtttctgt cgggcgtggc ggcttgcgcg gcggcagcgg tggcttggat
      361 tttcgagctg ctgcttggca gtggggcttt gggcgggctg cgggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgcct gacgcgcgcg
      481 ccggtgcagc cggtggtgtt tcacaggaaa aaataggttg gaacgggaaa tgccgtctga
      541 aacccgacac gcggtttcag acggcatgtt tttccgctaa cattacgcct gaatatggac
      601 aggaagcaga tatggaacgc aaagaacgcc tgcgtgcagg cattgccgcg atggggctgg
      661 atatttcgga aacggcgcag gacaggcttt tggtctatgt ggatttgttg aaaaagtgga
      721 acaaaaccta caatctgacc gccctgcgcg acgaggaaaa aatgattgtc catcatcttt
      781 tggacagcct gacgctgctg ccccatatcg agggtgtgca aacgatgctg gatgtcggtt
      841 cgggcggcgg tcagcccggc attccggcgg cggtgtgccg tccggatgtg caaataaccc
      901 ttttggatgc gaatacgaag aaaacggctt ttttacagca ggcggttatc gagttggggt
      961 tggacaatgt gcgcgtggta tccggacgcg tggaggcggt ttcggacgtg cgtgccgatg
     1021 tggttaccag ccgtgcgttt gcagaactgg cggattttgt gtcgtggacg gtgcatctgt
     1081 tgaaagacgg cggctactgg gcggcgatga agggcgtgta tccgcaggaa gaaatcggcc
     1141 gcctgccgca ggatgtgtgc gttgaaaaag tccaaaggct cgacgtgccg ggcttggatg
     1201 cggaacgcca tatcgtcatc ctgagcaagc gttgagcgca cttcagacgg catgaatacc
     1261 ttttttgtgc ggataaaggt aaaattccgc actgtttttc ttttttcaac atcagacggg
     1321 acacgggcgg gacatgagtg cgaacatcct tgccatcgcc aatcagaagg gcggtgtggg
     1381 caaaacgacg acgacggtaa atttggcggc ttcgctggca tcgcgcggca aacgcgtgct
     1441 ggtggtcgat ttggatccgc agggcaatgc gacgacgggc agcggcatcg acaaggcggg
     1501 tttgcagtcc ggcgtttatc aggtcttatt gggcgatgcg gacgtgcagt cggcggcggt
     1561 acgcagcaaa gagggcggat acgctgtgtt gggtgcgaac cgcgcgctgg ccggcgcgga
     1621 aatcgaactg gtgcaggaaa tcgcccggga agtgcgtttg aaaaacgcgc tcaaggcagt
     1681 ggaagaagat tacgacttta tcctgatcga ctgcccgcct tcgctgacgc tgttgacgct
     1741 taacgggctg gtggcggcgg gcggcgtgat tgtgccgatg ttgtgcgaat attacgcgct
     1801 ggaagggatt tccgatttga ttgcgaccgt gcgcaaaatc cgtcaggcgg tcaatcccga
     1861 tttggacatc acgggcatcg tgcgcacgat gtacgacagc cgcagcaggc tggttgccga
     1921 agtcagcgaa cagttgcgca gccatttcgg ggatttgctt tttgaaaccg tcatcccgcg
     1981 caatatccgc cttgcggaag cgccgagcca cggtatgccg gtgatggctt acgacgcgca
     2041 ggcaaagggt accaaggcgt atcttgcctt ggcggacgag ctggcggcga gggtgtcggg
     2101 gaaataggtc aatccaaatc gggctgcccg tgcctttatg ctgtttggcc gggtgcgtta
     2161 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagt
//
LOCUS       DAWISC010000214         2157 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWISC010000214 DAWISC010000000
VERSION     DAWISC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.214"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            136..2016
                     /locus_tag="WIW87_001950"
     CDS             136..2016
                     /locus_tag="WIW87_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ5175052.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPSISIYFDVDSE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTQSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          578 a          652 c          510 g          417 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc aacacggcaa gcgcaccaaa
      241 gtgaaggcaa acaatgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tatttcccca aaggaacagg cttccccgac cttccgcttc ctgaaatgcc tgacttaccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ctaccgaagt ggacgacgct
      901 ttaagcctga ccgatttggg caatggcata aaacgcgtcg gcatccacat cgccgcgccg
      961 tcgcttgcca ttaaacaagg cgacaaaatg gaaaaaaaca tcatggaacg cttgagcacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gtgcataccg cccttccatc agcatttatt tcgatgtgga cagcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gccgtcaaca tcgccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggatgag
     1381 gaaggcaaag tctccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcacccaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcgctg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacggcagat ggaagcctac tggagccttg tgtacctgca acagcaaggt
     1801 acaagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtcacgc gcgcgaccgg tattccgttt gacgcactgc ccaaatcaca ggcattgttc
     1921 aaaatcaccg aattggatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatc
//
LOCUS       DAWISC010000215         2123 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWISC010000215 DAWISC010000000
VERSION     DAWISC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.215"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            123..1508
                     /locus_tag="WIW87_001951"
     CDS             123..1508
                     /locus_tag="WIW87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ5175053.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDILVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WIW87_001952"
     CDS             1528..2046
                     /locus_tag="WIW87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ5175054.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          446 a          646 c          572 g          459 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acatactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgt ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca ttg
//
LOCUS       DAWISC010000216         2077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWISC010000216 DAWISC010000000
VERSION     DAWISC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.216"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            234..2009
                     /gene="nhhA"
                     /locus_tag="WIW87_001953"
     CDS             234..2009
                     /gene="nhhA"
                     /locus_tag="WIW87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HEZ5175055.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVKTAVLATLLF
                     ATVQASANNEEQEEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDGDTLNVGSKKDNKPVRITNVAPGIKEGDVTNVAQLKGVAQNLNN
                     RIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGN
                     WIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          649 a          499 c          520 g          409 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggca agtgctaaca atgaagagca agaagaagat ttatatttag
      421 accccgtaca acgcactgtt gccgtgttga tagtcaattc cgataaagaa ggcacgggag
      481 aaaaagaaaa agtagaagaa aattcagatt gggcagtata tttcaacgag aaaggagtac
      541 taacagccag agaaatcacc ctcaaagccg gcgacaacct gaaaatcaaa caaaacggca
      601 caaacttcac ctactcgctg aaaaaagacc tcacagatct gaccagtgtt ggaactgaaa
      661 aattatcgtt tagcgcaaac ggcaataaag tcaacatcac aagcgacacc aaaggcttga
      721 attttgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtattg
      781 gttcgacttt gaccgatacg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgctggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag acaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgggga cacattgaat gtcggcagca agaaggacaa caaacccgtc cgcattacca
     1681 atgtcgcccc gggcattaaa gagggggatg ttacaaacgt cgcacaactt aaaggcgtgg
     1741 cgcaaaactt gaacaaccgc atcgacaatg tggacggcaa cgcgcgtgcg ggcatcgccc
     1801 aagcgattgc aaccgcaggt ctggttcagg cgtatttgcc cggcaagagt atgatggcga
     1861 tcggcggcgg cacttatcgc ggcgaagccg gttacgccat cggctactcc agtatttccg
     1921 acggcggaaa ttggattatc aaaggcacgg cttccggcaa ttcgcgcggc catttcggtg
     1981 cttccgcatc tgtcggttat cagtggtaag ggctttttcg cctgtctgct gttgggacag
     2041 gcggaaggtt tgaagggaag ggtggcgatt tgccgcc
//
LOCUS       DAWISC010000217         2074 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWISC010000217 DAWISC010000000
VERSION     DAWISC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.217"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            340..852
                     /locus_tag="WIW87_001954"
     CDS             340..852
                     /locus_tag="WIW87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ5175056.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            932..2011
                     /gene="aroB"
                     /locus_tag="WIW87_001955"
     CDS             932..2011
                     /gene="aroB"
                     /locus_tag="WIW87_001955"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ5175057.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          508 a          655 c          510 g          401 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta tagtattgat aaacatatta
       61 tcttcaatat attcaattgg ataattgttt acctaagcaa agataattgc cttttcctga
      121 caaataagtg aaatcaacgg attgtcaaaa cacagcctga aataaaaaac ctccctgatt
      181 tcttttattt gtccttaaaa tcagaaaggt tcgggatggt cgggttattt ttccaaacgt
      241 accgccgccc tgccgatttc gtataaaatt ccgccgtaac ccgacaagcc cgaaccctgt
      301 cgccccgaaa ggcggggtgt caaacattaa ggaattgtga tgaaaaactt taacggcaaa
      361 ctcatcctca tcggactgat gggcgcgggc aaaaccacgc tgggccggca aatggcgcag
      421 cggctggatt accgttttta cgacagcgat cacgaaatcg ccgcagccgc cggcgttccc
      481 atccccacca tatttgaaat ggaaggcgaa cagggattcc gttcgcgcga aaccgccata
      541 ctcaaaaagc tggttatcct gccccatatc gtcctgtcca ccggcggcgg cgcggtgtta
      601 aaagaagaaa accgcgccct tatccgcaaa agcggcacgg tcgtctatct gcacgccccg
      661 cccgaaaccc tgctcgaacg cacgcgctgc gacaacagcc gtcctttgct gcaagttgcc
      721 gatcctttgg cgaaattacg tgaactctac gccgcacgcg accccgttta ccgccaaacc
      781 gccgacttta ccgtagaatc ggcaaactgc cgggaaaccg tgcaaaccct gctcaaacgc
      841 ttatcccgat aaaccggcat atgcgccgcg cccagaaaac caaaccgcgc cccgcccggc
      901 gggccggcgg ttcaaacttt aaggaacaac aatgaaaaca ctgaccgtac acaccccgtc
      961 ccacagctac cccatcttta tcggcaacgg actgctgccg caggcaggaa gcctgctcaa
     1021 accgcatttg ggcaaacgcg ccgccatcat cgccaacgaa accgtcgccc cgctctacct
     1081 cggcacgctt cagacggcat tggatgcggc aggcgtatcc catttcagca tcatcctgcc
     1141 cgacggcgag gcgcacaaaa actggcagac gctcaacctc atctttgacg ggctgatgca
     1201 aaaccgcgcc gaacgcaaaa ccacattaat cgcactgggc ggcggcgtga tcggcgacat
     1261 ggtcggcttt gccgctgcca cctaccagcg cggcgcaccg ttcgttcaaa taccgaccac
     1321 gctgttgagt caggtcgact catcggtggg cggcaaaacc gccatcaacc acccgctcgg
     1381 caaaaatatg attggcgcgt tttaccagcc gcaggcggtg cttgccgact tggacacgct
     1441 gcacaccctg cccgcccgcg aattgtccgc cggtatggcg gaagtcatca aatacggcgc
     1501 gctcggcgac atcggctttt ttgaatggct ggaacagcat atgcccgaac tgatgacgct
     1561 cgatcgggaa aaactcgccc aagccgtgta ccgctgctgc caaatgaagg cagacatcgt
     1621 cgcccaagac gaaaccgaac agggcatacg cgcatggctc aacctcggac acaccttcgg
     1681 acacgccatt gaaaccgaga tgggttacgg cacttggctg catggagaag ccatcgccgc
     1741 cggctgcgtg ttggcggcgc gtttgtccga acaactgggc aaaacctccg ccgcagatac
     1801 cgcgcggctc gccgccctgc tcgaagccgc cggactgccg tccgcgccac ccgtgtttgc
     1861 ctttgaaaaa tggctggaac acatgagcca cgataaaaaa gtcagcggcg gcatcatgcg
     1921 ctttatcggt ctgaaccggc tgggcgaagc caacatcacc gaaattaccg acacggacat
     1981 cctccgccgc accctgcaac cgtatctctg atttcctctg ccgatgtgct gccgcgcggg
     2041 tttgacgcac gatgatgatt ttccatcatc tttc
//
LOCUS       DAWISC010000218         1984 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWISC010000218 DAWISC010000000
VERSION     DAWISC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.218"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            305..1762
                     /locus_tag="WIW87_001956"
     CDS             305..1762
                     /locus_tag="WIW87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5175058.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          451 a          592 c          456 g          485 t
ORIGIN      
        1 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
       61 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      121 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      181 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      241 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      301 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      361 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      421 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      481 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      541 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      601 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg
      661 gctgatattc atcgcattgg gcagcggcgg cgtgaaatct acggccagtt ctatggtggg
      721 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      781 catcgccatc aacatcggcg gcttcctagg cccgctgctg accggcctac tgcaggaaaa
      841 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      901 ttattccctg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      961 acagggcaaa actgcggccg ccgtcggcat cgccctcatc gccgcacttg caaccgccat
     1021 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctattatcta ccgtcatcct
     1081 tgccgtcatc gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1141 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtctg
     1201 gtttcagatt tacaccgtgg caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1261 ttcgtttacc gtgcccgtcg cttggaaaga ttctatgcaa agcctgtggg tcatcctgtt
     1321 ttccggactg atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1381 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat
     1441 ttcctccggt acgccgatgc ctattgcggt tttcgcactg atcgtcctcg ccatcacgat
     1501 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1561 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1621 cggcgtattg tttgaaaaag gctatcaggc gggcgacgaa atcggcttct atcggctgct
     1681 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1741 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1801 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc gcccaaccgg
     1861 aaggcagcct gccgattgtc atttgaataa cgcaagggaa agccgttgat ttccgtttgt
     1921 atggaaacag tttggtttca ttggaaaaag gcattttgtc cgactaaatt agtgctgcat
     1981 caac
//
LOCUS       DAWISC010000219         1908 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWISC010000219 DAWISC010000000
VERSION     DAWISC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.219"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(221..1039)
                     /gene="cysE"
                     /locus_tag="WIW87_001957"
     CDS             complement(221..1039)
                     /gene="cysE"
                     /locus_tag="WIW87_001957"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ5175059.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1223..1801
                     /gene="grpE"
                     /locus_tag="WIW87_001958"
     CDS             1223..1801
                     /gene="grpE"
                     /locus_tag="WIW87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ5175060.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
BASE COUNT          437 a          436 c          580 g          455 t
ORIGIN      
        1 tcgattccgt actatccgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
       61 ccactatatt tccggttgcg tgggaatcgg gtgtattgaa taaaaggcat tttgtccgac
      121 tggcaagtgc cgacatcggc ggcatatcaa ggcgcaggct tgaagcgggc aatgtcgtct
      181 gaagcccgtt tggcgtttca gacggcattg gtgcggatat tcaaatcata aagtcgattt
      241 cggtaaactg gatattttga tccatatccg ccgacggtgt tttgagcgat cgcgccacgg
      301 gtttggcggg tacgccgaca accgtgatgg acggcggcac gtctgaaacc acgacgctgc
      361 ccgccccgat tttggcattg ctgccgatgc ggatattgcc caatatcgag gcgtttgcgc
      421 cgatcatcac gccgtcgccg attttagggt ggcggtcgcc gccttctttg cccgaaccgc
      481 cgagcgttac gccgtgcaaa atcgaaatat tgttgcccaa cacggcggtt tcgccggcaa
      541 caaagccggt ggcgtggtcg agcatcagcc cgtatccgaa acgggcggcg ggatggatgt
      601 ccacgccgaa tacttcggac atacggtttt gcaggaaata cgccagcgtt ttgcgcccgt
      661 cgagatacag ccgatggttg atgcggtgtg cctgaatcgc gtggaagcct ttgaaatata
      721 aaagcggcag cgaatattcg tcgcaggcgg gatcgcgttc gtagatggct tttaagtctg
      781 cttcgacgca tttgccgatt tgggtgtcgc tgcccaacgc ctgctggtag atttcaaaca
      841 gcgcgcgcac gtccataatc gggctgccga gtttgctgga aaggtggtag gcaaggacgg
      901 agccgaggga ctcgtggcgc aacacggttt ggtgcaaaaa acttgccagc atcggttcgg
      961 cggagaccgc ggccgcggtt tcttcgcgga tggtgtgcca gaggtcgaaa ccggttgtgt
     1021 ttaaatggtc ttttttcatg agtgatgacg tttgaaaatc gatatggtcg gcagtatctt
     1081 accgtctata ttattttttc ggtaggggat ttgaaaatga atttgaaatt ctctgctttt
     1141 gcttgaagtt tcttgaaaat gtccttatct tgcgcgggta ataactggat tttgatttcc
     1201 aatttgtttt aagggatacg atatgagcga acagacacag cagcaaaaca gtgaagaagc
     1261 ggttgaaaat gtggaggcgg tggaaaccgt cgagacagta ggaaatgcgg acggtgtgca
     1321 ggaacaggct gccgcagagc cggcttatga ggatttgcag gcgcggattg ccgagctgga
     1381 agcgcagttg aaagacgagc agctgcgcgc tttggcaaac gagcaaaacc tgcgccgccg
     1441 ccaccagcag gaaattgcgg atacgcacaa gttcgccgga cagaagtttg ccgtggaaat
     1501 gctgccggtc aaggattatc tggaaatggc gcttttggat cagagcggca atttcgatgc
     1561 gctgaaaatg ggcgtgcaga tgactttgaa cgagttgcag aaagcatttg atgctacgca
     1621 aatcaaggaa atcaacccta aagcgggcga taagctcgat ccgaatatcc atcaggcgat
     1681 gcaggcggtg gcaagcgaac aggagccgaa taccgtggtg ggtgtgatga agaagggtta
     1741 tacgctgtcc gaccgcgtgt tgcgcccggc tatggttacg gtggcgcaga aggaagcctg
     1801 aaggcgtctg gggaataatc tgatttattt cctgaagcgc gttttgcgta taaaccgatc
     1861 gaagtaaagc ggcaatgccg tctgaacccg cctgtcgggc ttcagacg
//
LOCUS       DAWISC010000220         1882 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWISC010000220 DAWISC010000000
VERSION     DAWISC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.220"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(127..1437)
                     /locus_tag="WIW87_001959"
     CDS             complement(127..1437)
                     /locus_tag="WIW87_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ5175061.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          449 a          481 c          494 g          458 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggagta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta ga
//
LOCUS       DAWISC010000221         1871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWISC010000221 DAWISC010000000
VERSION     DAWISC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.221"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            226..1698
                     /gene="pyk"
                     /locus_tag="WIW87_001960"
     CDS             226..1698
                     /gene="pyk"
                     /locus_tag="WIW87_001960"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ5175062.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENALIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          477 a          543 c          508 g          343 t
ORIGIN      
        1 ttatctagga cagccccaaa ataaatttag cctaagtcat aaaaccgtgt aattttgcgc
       61 caaaaaaggc ggtttgcagg taaaatatca aactattaat ttaaatcata tttttacaga
      121 atattccgcc gcgttcatca aatggacatc aaaccggttc caaatttctg taattttgta
      181 acaaaatacc gcaaaacacc cgattgagac caaaaggact ttcatatgaa ccagacaagc
      241 cgcgatctga cccgcatcag ccacaacact aaaatcgtcg ccacccttgg gccgggcagc
      301 aacaacgtcg aactgttgga agacatgatc cgcgtcggcg gtctgaacgt cgtccgcttc
      361 aacttcagcc acggcacgcc cgaattccat caggaaaacg ccctcatcgt gcgcgaggcg
      421 gcaaaacgcg ccggacagga aatcgccatc attgccgacc tgcagggccc gaaaatccgc
      481 gtgggcaaaa tcgccggcgg cggcatcgaa ttgaacaaag gcgaaacgct ggtactggat
      541 gccgcgctcg aaggcgaagg cacgcgcgag gcggtcggtt tggactaccg cgacctgccc
      601 gacgacgttg ccgcaggcga tgtcttgtgg ctggacgacg gcctgctgac cctgaccgtg
      661 gaatccgtcg aaggcagcag gattatcaca agggtggaaa acagccacgt cctgaaaagc
      721 aacaagggca tcaacaaacg cggtggcggt ctgtccgcag gcgcgttgac cgaaaaagac
      781 ttccgcgacc tgaaaaccgc gattgccatc ggttgcgact acctcgccat cagctttgtg
      841 aaatccgccg aagatttgca tatcgcgcgc gccaaagtcg aagaagaaat gaagggcagc
      901 acggccgtgc gccccggttt ggtttccaaa atcgaacgcg tggaagcgat tgaaaacttg
      961 gacgaaatca tcctcgccgg cgacggcatt atggttgcgc gcggcgactt ggcggtcgaa
     1021 gtcggacacg ccgccgtccc cgccctgcaa aaacggatga tccgccgcgc ccgcgagttg
     1081 cgccgcttca gcattacggc gacgcaaatg atggaatcga tgattaccaa ccccgtaccg
     1141 acccgcgcgg aagtcagcga tgtggcaaac gcggtattgg acggtaccga tgcggtgatg
     1201 tgttccgccg aaaccgccgt cggcgcgtat ccgtttgaaa ccgtcagcca aatggcgatt
     1261 atctgcgcgg ctgcggaaaa agagcaggat tcgctcaacg gcgttgccga acaggtcgag
     1321 tatcccgaag cggtcagcac caacctggcg gttgccggcg gtgcggtcag cgtggcgcgc
     1381 gcggttcacg ccaaagccat cgtcgccctg accgaaagcg gttcgaccgc ctttgaaatc
     1441 agccgccaca acatcaccct gccgattttc gcgctgaccc cgagcgtttc cgcccaacgc
     1501 cgtatggcga tgtaccgggg cgtgcgcccg ctgattttgg caaccagtac cgaccacgac
     1561 acggcgttaa acgaagtcga aacgatgctg gtggaacaca acatcctgca ttccggcgac
     1621 caatacatca ttaccagcgg ttcgcaaatg cgcgaatccg gttcgaccaa cacactggaa
     1681 gtgctgcgcg tcaaataatc cgccctgagt ggaaaatgcc gtctgaagcc gatgcccgag
     1741 gcttcagacg gcattttttg cggcggcgcg gcggttcggg caaactctga aaaaattgtg
     1801 caaatccggc aacatcggat aaaatcgagt acctatacta aagcgaaaca aggcatttcc
     1861 gactgccttt t
//
LOCUS       DAWISC010000222         1812 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWISC010000222 DAWISC010000000
VERSION     DAWISC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.222"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(58..381)
                     /locus_tag="WIW87_001961"
     CDS             complement(58..381)
                     /locus_tag="WIW87_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5175063.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(369..608)
                     /locus_tag="WIW87_001962"
     CDS             complement(369..608)
                     /locus_tag="WIW87_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ5175064.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(731..1528)
                     /gene="truA"
                     /locus_tag="WIW87_001963"
     CDS             complement(731..1528)
                     /gene="truA"
                     /locus_tag="WIW87_001963"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ5175065.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLVGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          405 a          430 c          520 g          457 t
ORIGIN      
        1 ccgtactggt ttaaagttaa tccactataa tgaacacaat ccattcagac tattcaatca
       61 ggcaaacatc tcctgcaata ctgcaaacag tttttcagcc gtactgttgt ctaaattgcc
      121 aagatgtttg accaatccgg ctttatccac agccctaatc tgttcgggca aaagcaaacc
      181 gtctttatcc tgaaagcgga cattgacgcg gaacggggca ggacggcttc cgctcgtcat
      241 gggaacgatc agcacagtct tgagatagtt gtgtatttca ggaggagaga ctacgacaca
      301 aggacgtgtc tttttgattt cgcttcctac ggtcgggtct aaggagacca gatagattcc
      361 gccgcgtact accatatcca ttctttatcc gcttcgtttt caatttcgga aaaaaaatgc
      421 tcctgctcgg tttcgacaag cattgcggca gcttctgccc atcccctgcg aacggtagga
      481 cagcttaaaa taatattgcc cttttcaact gtaacagcca agctgtctac tgcccctatt
      541 tgacccaata atgatttggg cagaatcacg ccttgcgagt ttcccatttt gcgtatgttg
      601 agaatcatat acgtaccata ttcacctgtt tatgtaataa caatgttagt accttgatga
      661 ggtagtgtca acatggaaaa agattgcctg acagtttgtc cgatttcaaa atctccgcga
      721 caagcatgtt ttaaagccat tcggggattt gggggcggat gatgccgtat gcctcgggat
      781 agtcgacgcc ggtcaggtaa agtccgtcgg gcatgaaggt cggcggggct ttgaggcggc
      841 tgcgttcttg aatcagtgcg gcgaagcctt cgacgctgag tctgccgctg ccgacataaa
      901 cgagcgcgcc catgatgttg cgtaccatgt ggtgcaaaaa ggcgttgccg tgcaaatcga
      961 ggcggacgag tcctgagctt tgggtaaggt cggcgcggta gatggttttg acgggggatt
     1021 ttgcttggca ttcggcggcg cggaagctgg agaagtcttg ttcgccgacc aataaggcgg
     1081 cagcctgccg catctgcccg atgtcgagtt tgaggtgtgt ccagcctgcc ctgtttttga
     1141 gcagggggga acggacgggg gcggattcga gcaggtagcg gtagtgccgt ccgtatgcgt
     1201 caaatcgtgc atgaaattcg ggggcgacct gtcgggcgtg caaaacggca atgccttcgg
     1261 gcaggtgggc atttacgccg cgcacccatg cctgttgggg acgggcggca gttgtgtcga
     1321 agtggacgac ttgggcggtg gcatgcacgc cggtgtcggt cctgccggca acggtggtgg
     1381 aaaccgcttc ccctgctatt tgggcgagcg cggtttccaa tgccgcctga acggtcggta
     1441 cgccgtcagc ctgtttctgc cagccgtaaa agcggctgcc gtcataggat agggttattg
     1501 cccagcgttg tttttgtgcg gtatccatcg gatttgggat tcggataaat gttcagactg
     1561 cattgtatcg cagattttgc agggaaacgg caaacgccca gggcgagcgg cgttgtttgg
     1621 ggagttgttg ggggggggga tgcagttgct acgaatcgct atcctgtgaa tttaccctgt
     1681 caggagtgcc cgaatcgtca ttcccgcgca ggcgggaatc taggacgtaa aatctaaaga
     1741 aaccatttta tagtggatta acaaaaacca gtacagcgtt gcctcgcttt agctcaaaga
     1801 gaacgattct ct
//
LOCUS       DAWISC010000223         1772 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWISC010000223 DAWISC010000000
VERSION     DAWISC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.223"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WIW87_001964"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WIW87_001964"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ5175066.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKTVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          481 a          401 c          448 g          442 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgt
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcagat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcgg aatacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaaa ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc cggtctggcg ttatacggca tttccccgtt cggaggaggc
      841 gatgacaggc tgaagcccgt gatgaggctt tcaacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgctgtcg acggcaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcataaa tgcgttttga caaaatcagt atagtggatt aacaaaaatc
     1321 aggacaaggc gacgaagccg cagacagtac aaatcgtacg gcaaggcgag gcaacgccgt
     1381 actggtttaa atttaatcca ttataaaaga cattgccaaa tagaaaatac tgataatgcc
     1441 cacaatctga aaccccatcc ttcccgcaaa aacggaaaat caaaatcaga aacctaaaat
     1501 ttcgtcattc ccgcgcaggc gggaatccgg ttttttgagt ttccgtcatt tctgataaat
     1561 tctcgtcgtt tttcatttct agattcccgc ctgcgcggga atgacaaaat taaagtttca
     1621 gaatttattt gaacaacaaa ctagattccc gctttcgcgg ggatgccggc ggataggttt
     1681 tgttgtttcg gataaattcc tgttgttttt tggtttccag attcccacct ttaaaagaat
     1741 gacgtaagaa agcagaaata aggacacagg ga
//
LOCUS       DAWISC010000224         1740 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWISC010000224 DAWISC010000000
VERSION     DAWISC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.224"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            30..917
                     /gene="prmA"
                     /locus_tag="WIW87_001965"
     CDS             30..917
                     /gene="prmA"
                     /locus_tag="WIW87_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ5175067.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTATQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            935..1498
                     /gene="orn"
                     /locus_tag="WIW87_001966"
     CDS             935..1498
                     /gene="orn"
                     /locus_tag="WIW87_001966"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ5175068.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDDVLNKMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          460 a          533 c          444 g          303 t
ORIGIN      
        1 aatccactat atttccaaga aagcccgtta tgccctacca acaaatcacc gtcaacgtca
       61 acgatgccgt cgccgaacgc ctcgccgacg cgctgatgga acacggcgca ctctccgccg
      121 ccatcgaaga tgcctacgcc ggcacgcaaa acgaacaggc gattttcggc gaacccggta
      181 tgcccgccga acaaatctgg cagcagagca aagtcatcgc cctgttcggc gaacacgacg
      241 aagccgccgc catcatccaa accgccacac aagaatgcgg gttaaaagac ttggcataca
      301 ccggcgaaac catcgaagac caagactggg tgcgtctcac gcaatcgcaa ttcgacccca
      361 tccggatttc cgaccgcctg tggattaccc cctcttggca cgaagtcccc gaaggcagtg
      421 ccgtcaacct ccgcctcgac cccggactcg ccttcggcac cggcagccac ccgaccacgc
      481 gcctctgcct caaatggttg gatacgcaac tcaaaaacgg cgaaagcgtc ctcgactacg
      541 gctgcggttc gggcatcctg accatcgccg ccctcaaact cggtgcaggt ttcgccgtcg
      601 gcgtggatat tgacgaacag gccgtccgcg ccggcaagga caacgccgcg caaaacaacg
      661 tcgatgcaca attcttcctg cccgacggtc tgcctcaagg gcaattcgac gtagttgtcg
      721 ccaacatcct cgccaaccct ttgcgtatgc ttggcgaaat gctcgccgcc cgcaccaaac
      781 agggcggacg catcgtgttg tccggtttgt tggacgaaca ggccgaagaa ctcggcggca
      841 tttacagcca atggttcgac ctcgacccgg cggaaaccga ggaaggatgg gcgcgattga
      901 gcggcgtaaa acgctgaaac ggaaaggaaa caccgtgcag gataaaaaca acctctgctg
      961 gctcgatatg gaaatgacgg ggctgaatcc cgaaaccgac cgcattatcg aagtcgcgat
     1021 gattattacc gactcggatt tgaatgtgtt ggcgcaatcc gaagtttacg ccgtccacca
     1081 aagcgacgac gtgctgaaca aaatggacga atggaacacc gccacacacg gcaggacggg
     1141 gctgacacag cgcgtacgcg aatcgtcgca taccgaagcc gaagtcgaac agaaactgct
     1201 ggactttatg tcggaatggg tacccggacg cgccacgccg atgtgcggca actccatcca
     1261 ccaagaccgg cgttttatgg tcaaatatat gccgaaactg gaaaactact tccactaccg
     1321 caacctcgac gtttccacgc tgaaagaact cgccaaacgc tggaatccgc ccgttgccaa
     1381 aagcgtcgtc aaacgcggtt cgcacaaggc attggacgac attttggaga gcatcgaaga
     1441 aatgcgccac taccgcgaac actttctgat ttccgccccg agagccgaag cgcaataaga
     1501 aacaaacaat gccgtctgaa acgcagtttg catttcagac ggcattttta cagcagattg
     1561 aaatcaaaaa tatacacgcc cgtcattccc gcacaggcgg gaatccggaa ggtcgggcct
     1621 gccgttattt tcaatcatta cagaaactga aaggtctgga ttcccgcctg cgcgggaatg
     1681 acgggcgtgt gcattcttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
//
LOCUS       DAWISC010000225         1721 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWISC010000225 DAWISC010000000
VERSION     DAWISC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.225"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WIW87_001967"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WIW87_001967"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ5175069.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRGLTEKYGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1394..1567)
                     /locus_tag="WIW87_001968"
     CDS             complement(1394..1567)
                     /locus_tag="WIW87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ5175070.1"
                     /translation="MKSERQHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          385 a          498 c          462 g          376 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac cctgtccgtc aaaacatcat tcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cggaaacgaa acctgcttca taagcggacg ggccgaaggc gacattgcgg tcgagcatcc
      181 cgtggaaaaa ctgtttgaag ccttcaatat tggaacgcgc catatcggca tagtttcgcg
      241 gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata gaagccttcg cgctggatga tttccagcgt tttcaagcct gcggcgacag
      421 caatcgggtt gcccgacaag gtgcctgcct gatacacgcc gcccagcggg gaaatacatt
      481 ccataatgtc tttgcgcccg ccaaacgcgg caagcggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcaaagattg cgcgccgccg agcgcgacgc
      601 ggaagccggt catcacttcg tcgtaaatca acaccgcgcc gtatttttcg gtcaatccgc
      661 gcaaggcttt gacaaaggct tcagacggcc taaccagatt catattgccg acaaaaggct
      721 ccaaaatcac acaggcaatg tcattgccgc tttgagcaaa ggcttcttcg agttgggcga
      781 tgttgttgta ttcgaggact aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 agggattacc gaaggtgagc agaccgctgc ctgctttcac caacaggctg tcggaatgcc
      901 cgtggtagca gccttcaaac ttgatgattt tgtcgcgacc ggtaaaaccg cgtgccaaac
      961 ggatggcggt catcgtcgct tcggtaccgg agctgacgag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttgt tcggcaatgg cgatttcgcc ctcggtgggc gcgccgaacg
     1081 acaaaccgcc caaggcagct tcgcgcacgg cttcgacgac ttcggggtgc gcgtgtccga
     1141 caatcgcagg cccccaagag ccgacatagt cggtataacg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgatgaagc gcggcacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga ccgccttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atttatcctc aaatgccgcc tgaacggcag gtttcgggct tagaagcaga
     1381 aagtcccatt ttatcatttt tcaggttgcg acaaggattt gcctgcttct ttgcggatga
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtcccggac aactccttcc cagtcgtaat ccacacacca ctcccaatgt tggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acctcgtgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacctcc gcccgggtcg
     1681 ttttgccttt aacaaccgtt tgtgcgattc cctcttccgt c
//
LOCUS       DAWISC010000226         1718 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWISC010000226 DAWISC010000000
VERSION     DAWISC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.226"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            342..1646
                     /locus_tag="WIW87_001969"
     CDS             342..1646
                     /locus_tag="WIW87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ5175071.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          351 a          431 c          509 g          427 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggata
//
LOCUS       DAWISC010000227         1691 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWISC010000227 DAWISC010000000
VERSION     DAWISC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.227"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(195..1373)
                     /locus_tag="WIW87_001970"
     CDS             complement(195..1373)
                     /locus_tag="WIW87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ5175072.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVA
                     GGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRH
                     DDMPVSVRYDSPEFSGFSGSVQFVPIQNSKSAYTPAYYTKDTNNNLTLVPAVVGKPGS
                     DVYYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSGSDQAKGTDPLKNHQVHR
                     LTGGYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFI
                     ERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF
                     "
BASE COUNT          438 a          429 c          388 g          436 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcgccattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcagacggt gtacctgatg gtttttcaag ggatcggtac ctttggcttg atcactcccg
      601 ctgccgatca agaacaactc aaaagcatta cgtccgacat tggcgtgtct cgcatattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gatccgggct tgccgacaac agccggaacg agagtaagat tattgtttgt atccttagta
      781 taataagccg gcgtataggc ggacttgctg ttttggatcg gaacgaattg aacgctgccg
      841 ctgaaaccgg aaaattcggg ggaatcgtag cgtacggaaa ccggcatgtc gtcgtggcgt
      901 ttgaaaatac ccaattgcga agccacatca ttattgctgt cccaaggatc aatggcttgg
      961 ctggcatcgt caaactgatt cgcaacgcga ccggcgcgca gcgtaccgaa ttcgcctgcc
     1021 aagccgataa aggattccct gttgccccac tgggtcgcgc cgccgccggc aacggatacg
     1081 tcttgctcaa gctgccaaac agccttcagc ccgtcgccca aatcctcact ccccttaaag
     1141 ccgataaacg agccgaaatc actgattttc gtcctgatgc ggcttttggc cttagtaact
     1201 ttagtaactt ttacctgacc gctcgctcca ccgttagcgg cttgtgcttc agtcaattgc
     1261 agctggtagt tcctgccttc cacgccggct ttgatttcgc cgtataggct gacatcggca
     1321 acggccgcaa gcggcagtgc ggacaatacg agggcggtaa gtttttttcg catatcggct
     1381 tccttttgta aatttgataa aaacctaaaa acatcgggca aacacccgat acgtcttcaa
     1441 ttataccccc cccccccgca aaaaaccatt tttcagaaca aatatctgat aaatgccgca
     1501 acctttattt taaaaatgat tatattttga tataaaacaa tagcttattt tttcaaaaac
     1561 gttgtgtttc tacaacacaa ttcaagcgca gacctcgtgc gagccgatgc gctgctgccc
     1621 ggatgcagtc tcggcttttt aaaacgccat aaaaaaacac acgcggcact ttatagtgga
     1681 ttaacaaaaa c
//
LOCUS       DAWISC010000228         1687 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWISC010000228 DAWISC010000000
VERSION     DAWISC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.228"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     regulatory      74..118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            123..596
                     /gene="queF"
                     /locus_tag="WIW87_001971"
     CDS             123..596
                     /gene="queF"
                     /locus_tag="WIW87_001971"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ5175073.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDAQ"
     gene            671..1357
                     /locus_tag="WIW87_001972"
     CDS             671..1357
                     /locus_tag="WIW87_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ5175074.1"
                     /translation="MYAFTAAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYAIGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT          402 a          510 c          357 g          418 t
ORIGIN      
        1 acggaaaacg ccgccgtcca aaccatgccg tctgaagaaa actacacaga taccgccgct
       61 tatattacaa tcgccgcccg tggttcgaaa acctcccaca ttaaaaaact aaggaaaccc
      121 tcatgtcccg caacaacgaa gagctgcaag gcatctctct tttgggcaat caaaaaaccc
      181 aatatccgac cggctacgcg cctgaaattc tcgaagcatt cgacaacaaa catcccgaca
      241 acgactattt cgtcaaattc gtctgtcccg agttcaccag cctctgcccc atgaccgggc
      301 agcccgactt cgccaccatc tacatccgct acattccgca catcaaaatg gtggaaagca
      361 aatccctgaa actctacctc ttcagcttcc gcaaccacgg cgattttcat gaagactgcg
      421 tcaacatcat catgaaagac ctcattgccc tgatggatcc gaaatacatc gaagtattcg
      481 gcgagttcac accgcgcggc ggcatcgcca ttcatccgtt tgccaactac ggaaaagcag
      541 gcaccgagtt cgaaacattg gcacgcaaac gcttgtttga gcacgacgca caataaacca
      601 tccttcccat atttcagacg gcattccttc gatacgatat gccgtctgaa cgcctttccg
      661 aaagattttt atgtacgcat ttaccgccgc acagcaacag aaggcactct tccggctggt
      721 gctttttcat atcctcatca tcgccgccag caactatctg gtgcagttcc ctttccaaat
      781 tttcggcatc cacaccactt ggggcgcatt ttcctttccc ttcatcttcc ttgccaccga
      841 cctgaccgtc cgcattttcg gttctcactt ggcacggcgg attatctttt gggtgatgtt
      901 ccccgccctt ttgctttcct acgtcttttc cgttttgttc cacaacggca gttggacagg
      961 cttgggcgcg ctgtccgaat tcaacacctt tgtcggacgc atcgccttag ccagctttgc
     1021 cgcctacgcg atcggacaaa tccttgatat ttttgtattc aacaaattac gccgtctgaa
     1081 agcgtggtgg attgcaccga ccgcatcaac cgtcatcggc aacgccttgg atacgctggt
     1141 atttttcgcc gttgccttct acgcaagcag cgatggattt atggcggcaa actggcaggg
     1201 catcgctttt gtcgattacc tgttcaaact taccgtctgc accctcttct tcctgcccgc
     1261 ctacggcgtg atactgaatc tgctgacgaa aaaactgaca accctgcaaa ccaaacaggc
     1321 gcaagaccgc cccgcgccct cgctgcaaaa tccgtaaaac agccctgccg ggaatcggga
     1381 cggttgggtt ttcttttatt tgcaaacact gtcggaacgg ataaatccgg attcccgccc
     1441 acttcgtcat tcccgcgaaa gcgggaatcc ggtacgttgg gtttcagtca tttccgataa
     1501 actcctgccg cgttgtattt ctagattccc gctttcgtgg gaatgacagc ggatgggttt
     1561 ccgtgccgtg gtgtcaggat tcccgcccgt acgggaatga cggcagtgct tccttggctt
     1621 agttttgttt taatcaactg catagtggat taacaaaaat caggacaaga cgacgaagcc
     1681 gcagaca
//
LOCUS       DAWISC010000229         1672 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWISC010000229 DAWISC010000000
VERSION     DAWISC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.229"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(20..1228)
                     /locus_tag="WIW87_001973"
     CDS             complement(20..1228)
                     /locus_tag="WIW87_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ5175075.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
BASE COUNT          483 a          406 c          272 g          511 t
ORIGIN      
        1 ttaatttttc ctttttaggt taattttgat ttgcatgaag atcatacatt ctgtcgagat
       61 aaagctgata ttgcctctca ctttctacat cgtgatgtgg atcgaaaagc cttttatccg
      121 gatcgaattt atctgattgc ttaaatttga taattccgag ttcttccaat tcaacctcta
      181 aatctacatc cggttgttcg tggataaagc cgaatttcaa agattccttc aatccggcca
      241 acgaatattt ttcaggttct agccctttgg gataccccaa atctgccttc agatatctga
      301 caatttcatc actatcaaaa cccatatcaa acatgaaatt aatcagtttg ccgaccgcgt
      361 tttttgcgta tctcaattta tgctgaaaag ttaaattagc cactttttta cggtaatcga
      421 acctttccgg attcggcata tttttaaatt tctgacaaat cgggaaagcg cctgaaaagt
      481 aagaaccttg atttatcaga atatccaaag gtatttccat atctccatga ttaaactgaa
      541 tttcgaacct tacccacttg ctttctttat cgcctagctg cctgcctttc tcataaacac
      601 gcacaaaacg agaatttttc ttgcgaccta cataaaatgt cttgccgctc ccgtcctctc
      661 tccgccaagc cgttccaacc atttcagatt tcggcctcat gttactgtta tcgaaaaaac
      721 cgttatcgtg atccaaaagt gcctgttccg gcgtgtactc cccatcaaaa aaatcaagtg
      781 ccaaatctac ccgcgttatc ctcggcctca atgaatcttc caaaaactgc ttaagcctca
      841 attcccaacc tggatttgca atgttgcaac ctacaccttt caattcgatt aaaaccgtat
      901 ttcgctgacc tccgtaatgg acttcgccgt agtcaacttc ttccgatccc aacctaaaca
      961 tcgaatcgta aaatttattg cccttcgatt tgcatctgct cgtgatgcca aaccctaata
     1021 tttcctccaa ttttttgctt aaaacaaaca tatattcggc atcggaaact aaggggcatc
     1081 cggaaacttt cagcaaggaa tcttcgtgca gtgtgaatga caaccaatct ataaaaacgc
     1141 cgtcctgcct gcccctacgt tgcggaattt ctaataactt cccattgccg ttagatatga
     1201 aatgggaaaa atattctgct tcactcattt tgttcagtac ctttagggat ttgttttatt
     1261 tcgctccccc cctgttagtc aggggggggc tttcagccgt ttcccgtctg ccgcgctaaa
     1321 gcgcgtccaa cggtcaacga ccgaaagccc aatcctgaca aactgttaaa gatcaagaag
     1381 aaagaccaca accgtctgtt gtgataatta ccggaaaatt cgagccaacc gaatctatat
     1441 aatcgaacgc ctgataaagc tttgaaaaat tttcttgttc agcgagttta tgcggttcac
     1501 catgcctgaa ctgatagaaa cataaaacgc aataatctga ttttttaaat attctccaat
     1561 aggaacaaga aaatattaca tttgctactg acataaaaaa gcccctttca cttggctgtc
     1621 aaaggggaat gttaagaaaa gtaatgcgcc cctttgatag agcgcatcat at
//
LOCUS       DAWISC010000230         1670 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWISC010000230 DAWISC010000000
VERSION     DAWISC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.230"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(11..1225)
                     /locus_tag="WIW87_001974"
     CDS             complement(11..1225)
                     /locus_tag="WIW87_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ5175076.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKK
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
BASE COUNT          515 a          402 c          238 g          515 t
ORIGIN      
        1 ttgatttcct ttaaaaataa tcataatcta cattttgatg ataaacttta cttaaatatt
       61 cctgatattt cctttctttc tcgacatcat aatcaggact aaaaagcctt ttttccctat
      121 cgaatttgtc agaattttta aaagcaataa cccccaattc atcaagttca atttccaaat
      181 caatatccgg ctgttcatga ataaaaccgt gtttcaaacc gtcccttaac atttccagag
      241 catatttttc aggttctaat cctttgggaa atcccgaatc tgcctttaaa gattccacaa
      301 tttcgctatt atcaaaaccc atttcaatca tgaaattgac cagttttcca accgcgtttt
      361 tcgcgtaatg caatttatgc tcgaaagtta aattaagctt tttctttctc tgatcaaacc
      421 tttcgggaac cggcatattt ttaaattttc tacaaattgg aaaagctcca cagaaatacg
      481 aaccctgatt tattaaaata tccaagggta tttctatatc tccataatta aactggatct
      541 cgaaccttac ccatttgctt tctttatctc caagctgcct gcctttctca taaacacgaa
      601 caaaacgaga atttttcttg cgacctacat aaaatgtctt gccgctcccg tcctcattcc
      661 gccaagccgt accgatcgtt tcagatttcg gcctttgatt gctgttatca aaaaaaccat
      721 tatcgtgatc taacaacgcc tgatccggcg tgtactctcc atcaaaaaaa tcaagtgcta
      781 ggtcaattcg cgttattctt gtccttatcg aatcatcgag aaactgcttt agcctcaact
      841 cccaacccgg acttgcaacg ctgcaaccag tacctttcaa ctcaactaaa acagtattgc
      901 gctgacctcc gaaatgcacc tctccataat caacatcatc cgaacctaac ctatacatgg
      961 attcatagaa tttgttgccc cttgatttgc atttgcgcgt tatgccaaaa cctagaattt
     1021 cttccagctt tctgcttaat acatacatgt attcagcatc agaaaataaa gggcaaccgg
     1081 aaactttcag taaagtatct tcatggaatg tgaatgaaat ccaatcaaca aaaaccccgt
     1141 cttgcttacc tcttcgctgc ggaatttcta aaagcttccc ttttccgtcc gatataaagt
     1201 gtgagaaata ttcaacttca ctcattcgtt cactactctt ggaattggtt tttaatttcg
     1261 ctccccccct gttagtcaag gggggggctt tcagccgttt cccgtcttcc gcgctaaagc
     1321 gcgtacgacg gtcaacgacc gaaagccaaa tcctgacaaa cttttaaaga tcaacaaaaa
     1381 agaccacaac catccgttgt gataatcact ggaaaattcg aaccaaccga atctatataa
     1441 tcgaacgcct gataaagctt tgaaaaattc tcttgattaa tgagtttatg cggttcgcca
     1501 tgtctgaact gataaaaaca caaaacacaa gaatctgatt ttttaaatat tctccaataa
     1561 gaacaggaaa atattacatt tactgctgac ataaaaaagc ccctttcact tggctgtcaa
     1621 aggggaatgt taagaaaatt aatgcgcccc tttgatagag cgcatcatat
//
LOCUS       DAWISC010000231         1652 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWISC010000231 DAWISC010000000
VERSION     DAWISC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.231"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            119..1492
                     /locus_tag="WIW87_001975"
     CDS             119..1492
                     /locus_tag="WIW87_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ5175077.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          427 a          533 c          394 g          298 t
ORIGIN      
        1 ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt ttgttaatcc actatatttc
       61 cccgtcctat cggtttcccg tttcagacga cataaggtct gaaagaaaga ctacaattat
      121 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      181 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      241 tgatttgcta gccgccgcgc aaaccggcac aggcaaaacc gccgccttta tgctgcccag
      301 tctggaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      361 tatgctcgtc cttaccccca cgcgcgaact tgccgaccaa atcgaccaaa acgtgcagtc
      421 ctacatcaaa aacctgccgc ttcgccacac cgtcttgttc ggcggtatga atatggacaa
      481 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccaccgtcg gacggctgct
      541 cgaccacgtc aaacaaaaaa acatcagttt gaacaaagtc gaaatcgtcg ttctggacga
      601 agcagaccgc atgttggata tggggtttat cgacgacatc cgcaaaatca tgcagatgct
      661 gcccaaacaa cgccaaaccc tgctcttttc cgctaccttc tccgcaccga tacgcaaact
      721 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      781 cgccaacgtc gaacaacaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      841 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      901 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      961 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
     1021 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1081 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1141 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1201 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1261 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa accgaaatgc gcgaaccgag
     1321 acaacgcaac cgctacgaat ccgccagggc tcagcgcgaa aaaaataccc gaccggaaac
     1381 cacggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1441 tcgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat aaccctgaaa
     1501 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaaccg gactgacgca
     1561 tcgggagcaa ccgcccgcac cggataaatt tctgccgcaa acagtttcag acggcatttg
     1621 ccgcctgtac aatatagtgg attaacaaaa at
//
LOCUS       DAWISC010000232         1646 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWISC010000232 DAWISC010000000
VERSION     DAWISC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.232"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(18..680)
                     /locus_tag="WIW87_001976"
     CDS             complement(18..680)
                     /locus_tag="WIW87_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5175078.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLDEYDSRYAQDTTLFDGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMTTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(718..1545)
                     /locus_tag="WIW87_001977"
     CDS             complement(718..1545)
                     /locus_tag="WIW87_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ5175079.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAINLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT          316 a          436 c          506 g          388 t
ORIGIN      
        1 tgtggggcgg atatttttca gggctgtatt ttgtccagac attcgagcag atcgagtggc
       61 gtgcggatgt ggaaatccgc ctgccatgag ccggtatcgt cttcgggagc gatgtagccc
      121 cattcggcga ggacggtcgt cataccggcg ttgcgccccg cctgtatatc gcgttccgcg
      181 tcgccgacgt agagtgtgtg ttgcgggtcg gcgtggattt gtccgcacgc atacagcatg
      241 ggtttgacgc tgggcttggg ctcgccgcag gtgtcgccgc tgacgacgac ggcgggtggg
      301 atgatgaagc cgagtttggg gacgagtttg tcggtgaagc gcatgggttt gttggtgatg
      361 atgccccatt tgatgccgcg ttttccgagt tcggcgatga gttcgtttac gccgtcgaag
      421 agggtggtgt cttgggcgta gcggctgtcg tattcgtcaa ggtattcggt gcgccatcgg
      481 gcatagtcgg gatggtcggg ggtgatgcct gcgccgagct tgatcagtcc tgccgcgccg
      541 tggctggctt gggtgcggat ttcgtccatg ctttttgcag gtagtccgtg gcgggcgagc
      601 agggtgttga gtgcgccgcc gaggtctagg gcggtgtcgg cgagcgtgcc atcgaggtcg
      661 aacaatacgg cttgtatcat gtgtgttcct tttttataaa gtgcgggacg aagggtttca
      721 gacggcatgt ttattttgtt tcaaaccctg ctcgaaatct tccaacatat ccaattcaaa
      781 gcggctgaag cctgcttttt cgcgcgcttc gatgttcaca tagccccgga agataaacat
      841 atcgtaacgg gcaatcaggc tgcggaacag ggcgacaggc tccaaaccgc gttcgcggca
      901 aaggtgttga taccaccggt tgccgatggc gacgtgtccc acttcgtcgc ggtaaatgat
      961 gtccaacacg ccgcaggttt ccgaatcacc gcgctgcgcc accttcgcgc gtatgccggg
     1021 cgtaacgtcc agcccgcgcg cttccaaaac gcgcggcact aaagccatac gcaacaaagg
     1081 atcgtaggcg gttttgtatg ccatatccca taaatgattg tgtgcttcaa aatcgccgta
     1141 atcgaagccg aaagcgcgca gcctttcgcg catcaggcgg aaatggtaca cctcttcctt
     1201 cgccactttc acccagtcgc ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc
     1261 gtccaaagcc agattgatgg cgttgaattc gatatgcgca atcgcgtgca gcatcgccgc
     1321 atagccttcg gttgtgttca ttttgcgtgg cgtcagctgc gacggcgcga ccaaaacagg
     1381 cttgtccggt cgtcccgcgc gggggaagtc cgccggcggt gcgtttgttt ccgccccgtc
     1441 cgcattttga acggcggcaa acgcctcatc cgtcagccgt cctttttcat ctgggtcgcc
     1501 cgaaagcagg gcgcgttcca gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg
     1561 ttcggaaaac aaatattata gcgtttaaaa aaaacaagat gaggcatata atctccgcga
     1621 ttcggcattc cgcgcccaaa ccgtca
//
LOCUS       DAWISC010000233         1645 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWISC010000233 DAWISC010000000
VERSION     DAWISC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.233"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            63..1505
                     /gene="aldA"
                     /locus_tag="WIW87_001978"
     CDS             63..1505
                     /gene="aldA"
                     /locus_tag="WIW87_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ5175080.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
BASE COUNT          415 a          466 c          435 g          329 t
ORIGIN      
        1 tacggcaatt tatttgggaa taaactaaaa ctacatctaa ctacaaaact ggagaacccg
       61 aaatgaaaca attggccatg tacatcaacg gacgctttga aaacgatttc aacggcgaat
      121 ggcgcgacgt attgaacccg tccaccgaag aggccatcgc ccgcgaaccc aaaggcggca
      181 gggcggacgt tgaccgcgcc gtcgcggcgg cgcgtgcggc gcaaccggct tgggagcgtc
      241 tgcctgcggt cgaacgcggc gcgtatttgc gcaaaatcgc ccaaggcata cgcgaacgcg
      301 ccgacgagct gaccgacacc atcgttgccg aaggcggcaa aaccaaagac ttggcgcgcg
      361 tggaagtgat gttcaccgcc gactatctcg attaccaagc cgaatgggcg cgccgttacg
      421 aaggcgaaat catccaaagc gaccgcccgc gcgaaaacat tttattgttc aaacgtccgc
      481 tgggcgtaat tgccggcatt ttgccgtgga acttcccttt cttcctgatt gcccgcaaaa
      541 tgggtcccgc cttagtaacg ggcaacacca tcgttgtcaa acccagcagc gtaaccccga
      601 tcaactgcca catcttcgcc gaaatcgtcg atgcggtcgg attgcccgca ggcgtgttca
      661 acgtggtgaa cggtccgggt gcggaaatcg gcaatgcctt gtccgcccat ccgcaagtcg
      721 atatggtcag cctgaccggc tccgtcgaag cgggccgcca agtgatggaa gccgcttccg
      781 ccaacatcac caaagtttcg ttggaactcg gcggcaaagc ccccgccatc gttttgaaag
      841 acgcggattt ggatttggcg gtgaaatcca tcttggcttc gcgtgtcggc aacaccggtc
      901 aaatctgcaa ctgcgccgag cgcgtttatg tccacagcag tctgaaagac gcattcattg
      961 aaaaaatgac tgccgccatg aaaggcgtgc gctacggcaa ccctgccgaa gccgaagcag
     1021 gcgcgctgga aatgggtccg ctgattgaag agcgcgccgt caaagccgtt gccgaaaaag
     1081 tggaacgggc agtcaaacaa ggtgcgaaac tggtctgcgg cggcaaacgc gccgaaggac
     1141 gcggttattt cttcgagccg accctgctga ccgacaccga caacagtatg gacattatga
     1201 aagaagaaac cttcggcccc gtgctgcccg tttccgcttt cgacacgctt gaccaagtca
     1261 tcgcactggc taacgattgc gagtttggtc tgaccagttc tgtttatacg actaatttaa
     1321 acgaagcctt ctacgttacc cgccgcctgc aattcggcga aacctacatc aaccgcgaaa
     1381 actttgaagc catgcaaggc ttccacgcgg gctggaaaaa atccggcatc ggcggcgcgg
     1441 acggcaaaca cggtttggaa gaatatctgc aaacccaagt cgtttatctg gaaaccgaca
     1501 tttaatgccg ctttaaaacc ccgatagaaa atgccgtctg aacccgtttt caggttcaga
     1561 cggcattttt acagtttcac cggcaatcag tcatgaccga catcgatgtt tttgtctttg
     1621 tatagtggat taacaaaaat cagga
//
LOCUS       DAWISC010000234         1569 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWISC010000234 DAWISC010000000
VERSION     DAWISC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.234"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(116..1249)
                     /gene="carA"
                     /locus_tag="WIW87_001979"
     CDS             complement(116..1249)
                     /gene="carA"
                     /locus_tag="WIW87_001979"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ5175081.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLIESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          324 a          386 c          482 g          377 t
ORIGIN      
        1 gcgatggttc ggtcaatgtc ggcaacagtt agtactagat ggtcgagtgc gctaatcttc
       61 atttccagct ttgtttttca gacggcagca gcatactgtt gccgtctgaa aaccattatg
      121 cccgttttgc cgctttcata ttgccaatga atttgtcaaa caaatagccg acatcttgcg
      181 gaccggggct ggcttcgggg tggccttgga agcagaacac aggtttgtcg gtcagctcga
      241 tgccttgcaa agtgttgtca aacaaggatt tgtgggtaat gcgtgcgtta gcgggcaggg
      301 tgtcggcatc aacggcaaaa ccgtggtttt ggctggtgat gacgactttg ccgctgtcca
      361 aatcttgcac agggtggttc gcaccgtggt ggctgaagcg cattttcagg gttttcgcgc
      421 cgatggcgag gctgatgagc tggtgtccca agcaaatgcc aaaaatcggt ttgccgcttt
      481 ctatcagttt ttgtacggct ttgatggcgt aggtgcaagg ctcggggtcg ccggggccgt
      541 tggataggaa tacgccgtca gggttgagtg ccaacacgtc ttccgcgctc gtttgggcgg
      601 ggacgacggt caggcggcag ccgcgcgagg cgagcatacg caggatgttg gttttcacgc
      661 cgaaatcgta ggcgacgacg tggtaaggct gttcgtcagg ggtaacgaaa cccttgccca
      721 atgcccattc gccttccgtc cattcgtaag tttccgtgca ggaaacttct tttgccaaat
      781 cttttccgac catgctgccg aacgcggcga tgagttcttg cgctttttcg attgtggcat
      841 ccgcaccggt cagaatcgca ccgccttgcg cgcctttttc gcgcaacagc gtggtcaggc
      901 ggcgggtgtc gatgtcggcg atggcgacgg ttttgttgcg taccagatag tcgtgcaggc
      961 tttcggaggc gcggaagttg ctgtgcaaga gcggcaggtc gcggataatc aggccggcgg
     1021 cataaacgct gcggctttct tcatcttcgg cgttggtgcc ggtgttgccg atgtgtgggt
     1081 aggtgagggt aacgatttgt ttgcagtagg acgggtcggt caggatttcc tgatagccgg
     1141 tcatcgaagt attgaacacg acttcgccgg aagtcgaacc ttcgtaaccg attgatgtgc
     1201 cgtgaaatac gctgccgtca gcgaggacga ggagggcggg ggtgctcatg atgggaatcc
     1261 tgtttttaat aaaattcttg acaatgccgt ctgaagggac ttcggcaggg ggcatccggt
     1321 cggaaaaatc cggtcaaaaa aaaacacgcc gcgcaagata gacgcgtaac gtgcttttcg
     1381 ggcaagtgcc tataccctga aagcagcata ttttaagacg aaacataccc gatttcaagc
     1441 gcaaacgcgg caggcgggct gatatgttcg cgcgaaaacg gaaatccggg gcataaaacc
     1501 gcccgctccg cgcccactat gcccgttacg catttcccca cgtccgcccg gcgaaactat
     1561 gggaatacc
//
LOCUS       DAWISC010000235         1540 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWISC010000235 DAWISC010000000
VERSION     DAWISC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.235"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            33..1433
                     /locus_tag="WIW87_001980"
     CDS             33..1433
                     /locus_tag="WIW87_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ5175082.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          410 a          490 c          367 g          273 t
ORIGIN      
        1 tatccaccgt ccgccttttt acggaaaccg aaatgacccc ttccgcactg aaaaaaaccg
       61 tcctgctgct cggcactgcc tttgccgccg catccgtcca cgcatccggc taccacttcg
      121 gcacacagtc ggtcaacgcg caaagcacgg caaatgccgc cgccgcagaa gccgccgacg
      181 catcgaccat cttctacaac cctgccggcc tgaccaaact cgacagcagc cagatttccg
      241 tcaacgccaa catcgtgctg cccagcattc attatgaggc ggattccgcc accgacttta
      301 ccgggcttcc cgtccaaggt tcgaaaagcg gcaaaatcac caaaaccacg gtcgcgcccc
      361 acatctacgg cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc
      421 ccttcggctc tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac
      481 tcggtctgac cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaccgccatt
      541 ccttcggcgc aggcatcatc gcccaacata cttccgccga actgcgcaaa tatgccgact
      601 gggggattaa gagtaaagca gagatattga cggcaaaacc gcccaaacct aacggtgtag
      661 ccgaagctgc aaaaattcag gccgacggac acgccgatgt caaaggcagc gattggggct
      721 tcggctacca actggcgtgg atgtgggaca tcaacgaccg tgcgcgcgtg ggcgtgaact
      781 accgttccaa agtctcgcac acgctcaaag gcgatgccga atgggcggca gacggcgcgg
      841 cggcgaaagc aatgtggagt acgatgcttg cagcaaacgg ctacacggcg aatgaaaaag
      901 cccgcgttaa aatcgttacg cctgagtctt tgtccgtaca cggtatgtac aaagtgtccg
      961 ataaagccga cctgttcggc gacgtaactt ggacgcgcca cagccgcttc gataaggcgg
     1021 aactggtttt tgaaaaagaa aaaaccgtcg tcaaaggcaa atccgaccgc accaccatca
     1081 cccccaactg gcgcaacacc tacaaagtcg gcttcggcgg ttcttatcaa atcagcgaac
     1141 cgctgcaact gcgcgccggc atcgcttttg acaaatcgcc cgtccgcaac gccgactacc
     1201 gcatgaacag cctacccgac ggcaaccgca tctggttctc cgccggtatg aaataccata
     1261 tcggtaaaaa ccacgtcgtc gatgccgcct acacccacat ccacatcaac gacaccagct
     1321 accgcacggc gaaggcaagc ggcaacgatg tggacagcaa aggcgcgtct tccgcacgtt
     1381 tcaaaaacca cgccgacatc atcggtctgc aatacaccta caaattcaaa taaacggccc
     1441 gccgtttgaa tgtaacaatg ccgtctgaaa cagatttccg tttcagacgg cattgtttta
     1501 ggctcgattt atagtggatt aacaaaaatc aggacaaggc
//
LOCUS       DAWISC010000236         1512 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWISC010000236 DAWISC010000000
VERSION     DAWISC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.236"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(147..1142)
                     /gene="porB"
                     /locus_tag="WIW87_001981"
     CDS             complement(147..1142)
                     /gene="porB"
                     /locus_tag="WIW87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ5175083.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVFHQNGQV
                     TEVTTATGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQEGLNIEKYQIHRLVS
                     GYDNDALYASVAVQQQDAKLTDASNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGLV
                     DDADIGNEYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          385 a          339 c          389 g          399 t
ORIGIN      
        1 aaaaagccaa acagaaaaca gcgaaacaga aaacagcgaa acagaaaaca gcgaaacaga
       61 aaacagcgaa acagaaaaca ggaaaaagcc aaacagaaaa aagcctgtct ggcgacaggc
      121 tttttgttga taccaatctt tgcagattag aatttgtggc gcagaccgac accgccggca
      181 gtcgctacga atttgttttc gcctttgcct tcttgcaacc aaccggcaga aaccaaggca
      241 gaagtgcgtt tggagaagtc gtattccgca ccgacaacca cttggtcgta ttcgttgcct
      301 atgtctgcat catcaaccaa acctttgaag ccgtgggcgt aagaaactcg gggcgttacg
      361 ttgccgaagc ggtatgccaa ggtagcggca acttcggttt gagagttgtg cgaattggaa
      421 gcatcagtca gtttcgcgtc ttgttgctgt acggctacgg aagcgtacag ggcatcattg
      481 tcgtaaccgc tgaccaaacg gtgaatctgg tatttctcaa tattcaagcc ctcttgcact
      541 tgatgatgtc ttttataggc accgccatat tgcacgaaga agccaccgtt tttgtagttg
      601 aagccggcgt ggtaagattc gctgttatgt ctgcctgcat tgtcgttaag cgcgtattgt
      661 acgctgccgc tgaggccggc aaattcggga gaatcgtagc gtacggaaat gaggcgtgcc
      721 tcgggttcgg caattttgtt tacacccaaa tagtcgcttt tgctatccca aggattgatg
      781 tcgccggtgt ctttcaggac gctgttcaaa cgaccgacgc gcaatttacc gaagccgcct
      841 ttcaagccga tgaaggattg gcggttgccc caaccggagt cagtaccggc gatagatgct
      901 ttttgctcaa cctgccaaat ggctttcagg ccgttaccga ggtcttcttg gcctttgaag
      961 ccgattttcg aacccaaatc aacgatgccg gtagcggttg taacttcagt aacttggccg
     1021 ttctggtgaa atacagagcg ggaagtttct acgccggctt tgatggtgcc gtacagggta
     1081 acgtcagcca ttgctgcaac aggaagggct gccaaagtca gggcaatcag ggattttttc
     1141 attgctgtat tccttttttg gttaagaaat ttaagccggc cgggctttcc aagccgctta
     1201 gctttgcatt taccgccgac gttttcggcg attccatgtc tgaaactata gatgttcctg
     1261 tctgaaaaaa caaacttctg cctgtttttg tgtggaattc gatgtgtatt ttgaagggcg
     1321 gatagagata ttatgggtat tttttgtttt ataacatatg gttatttaaa ttttttaaga
     1381 tttgcatttt tacaacactt actcgggagg gtattggagg gcattgcaaa ccgggggtta
     1441 taaagacggc aaaaaacgcg cacgtggttt ctttcggaac gggcggatgc aaaacccgcc
     1501 cctgcggcag ga
//
LOCUS       DAWISC010000237         1494 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWISC010000237 DAWISC010000000
VERSION     DAWISC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.237"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(161..532)
                     /locus_tag="WIW87_001982"
     CDS             complement(161..532)
                     /locus_tag="WIW87_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175084.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMASKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(664..1107)
                     /locus_tag="WIW87_001983"
     CDS             complement(664..1107)
                     /locus_tag="WIW87_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175085.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1195..1419)
                     /locus_tag="WIW87_001984"
     CDS             complement(1195..1419)
                     /locus_tag="WIW87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175086.1"
                     /translation="MMKNKYILIKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
BASE COUNT          480 a          274 c          223 g          517 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tctgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agatgccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa ataataagga atatatatat gcatattcct tactattttt tcacaaatta
      661 tgcttatcta tgcttatttc cataaaaagc atgccctcga attttttgac ttgagttctg
      721 gaaatgagaa taatttgaat ggatttcctt ctgcccatgt ctaataacat tttgtcttcc
      781 ccaagcttgg tttgctaccg ctcttgcttg ttgagatgtt cgtgcataga cattagctcc
      841 tcttgataaa gcttgtgctg caacacgctg cgtaacccaa cgctgcgcaa ggaatctgcc
      901 cgcatgcaaa attcctattg ccaccaacgg tgccgcagcc ccttcagttt ctttcatctc
      961 cttttgagaa agctccgcca actgcatcgg cgcatctgcc cgcgtgtgga acacttggtc
     1021 ttcaaatgcc tgattgtcca atccgtttgc cattgcgggg gcgattatag acagcatcat
     1081 cgtggcttca gtgatttgtt ttttcataac aaattcttag ggaggagata atgttccatc
     1141 aaaatcctcc tgaaaggctg cctgaaattt ccaagcagcc tttttgattg cttattaatc
     1201 ggatagtgcg acaaatataa agtgggcggc aatcattgcc gtaacggcag ccataactgt
     1261 tttattttct tcaaaccaaa gcaagttgag aaacataaac atcaagaaat atgatggttc
     1321 ttcagttttg ccgctgcttt cttgagttag aagataaaat atagatatgg cggttatgct
     1381 gattgctgct tttattaaaa tatatttgtt tttcatcatc ttgtatggtt tttcatgcaa
     1441 gtttcaacaa taaaattcgg aataagatag agagatgttg atatatcaga taaa
//
LOCUS       DAWISC010000238         1448 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWISC010000238 DAWISC010000000
VERSION     DAWISC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.238"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            128..1258
                     /locus_tag="WIW87_001985"
     CDS             128..1258
                     /locus_tag="WIW87_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ5175087.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          384 a          344 c          343 g          377 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgacaat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttcgga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg gggcgatttg gaacggcttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcagtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg agccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccgtccgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt gaaattgctc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ccccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgttta gtttcggtca tttccgataa
     1381 attcttgcaa ctttgcgttt ctagattccc actttcgtgg gaatgacggc ggagggttgc
     1441 tgttttcc
//
LOCUS       DAWISC010000239         1442 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWISC010000239 DAWISC010000000
VERSION     DAWISC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.239"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            26..1363
                     /locus_tag="WIW87_001986"
     CDS             26..1363
                     /locus_tag="WIW87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ5175088.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWLYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          267 a          349 c          378 g          448 t
ORIGIN      
        1 atcttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
       61 tttcgtgctt gctgcggcag gttcggccat cggtttgggc gcgatttgga aatttcctta
      121 tacggcaggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttaccgtctt
      181 ggtcgcccta cccgttcagc ttgccgaatt ttatatcggg cgcacaggcg gtaaaaatgc
      241 cgtcgattcc ttcagggctc tgcgtccgag tacgcaatgg ctttgggtcg ggcgcatggg
      301 cgtttccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
      361 ttatgtcgtc cacagtttca cgggggcggt tcataccggc gcggactttg aagccctgtt
      421 cggcacaacg atttccaatc cggcaggttc gctgtcctat caggcactgt ttatgctgat
      481 tacggtttgg gtggtcaaag gcgggatttc agacggcatt gaaaaggcaa accgttatct
      541 gatgccgggg ctgtttatcc tctttattgc actggcaatc cgttcgctga cgctgccggg
      601 ggcaatggag ggcgtgtctt tcctgctcaa accgaattgg ttgtacttta aagccgatac
      661 gatgattacg gctttaggcc aggcgttttt tgccttgagc atcggcgttt ccgccatgat
      721 tacctacgct tcgtatttgg gaaaagatca ggatatgttc cgttccggcc atacgattat
      781 gtggatgaac ctcttggttt cgctgcttgc cggcctggtg attttcccgg cggtattcgc
      841 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc ccgcagtgtt
      901 tatgaagatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
      961 cacgctgact tcggcatttt cgatgttgga aacggtcatt gcctcaacca tccgccaaga
     1021 cgagcgcaaa cgcaaaaaac acacttggct tatcggcacg gccattttca ttgtcggcat
     1081 cccatccgcg ctgtctttcg gcgtatgggg cgagtttaag gttttcggca aaaccatttt
     1141 tgatttgtgg gactatgtta tttccgccgt cattatgccg attggtgctt tgagtgtttc
     1201 catctttacc gcctggattc aggacaagca gtctgtgtta aaagatgccg gcgcgggcag
     1261 caccgtacca cgggcagtgc tgctgctgtg gctgaatacc ttgcgctacc ttgccccgat
     1321 tgccattatt attgttttca tcaattcttt ggacatcctt taaaagccat ccaaacagca
     1381 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt cgggttcagc ctatttcgtt
     1441 ca
//
LOCUS       DAWISC010000240         1419 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWISC010000240 DAWISC010000000
VERSION     DAWISC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.240"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(209..1132)
                     /locus_tag="WIW87_001987"
     CDS             complement(209..1132)
                     /locus_tag="WIW87_001987"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ5175089.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          373 a          348 c          357 g          341 t
ORIGIN      
        1 aacctcaaat cccgtcattc ccgcgcaggc gggaatccag acttgtcggt gcggacgctt
       61 atcggataaa acggtttctt gagattccgc gtcctggatt cccactttcg cgggaatgac
      121 gaattttagg tttctgtttt ggttttttgt ccttgtagga atgatgaaaa tttaagtttt
      181 aggaatttac cggaaaaaat agaaagcgtt atccacaagt tctgatgttc agctcgtgaa
      241 atgcgtcggg caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc gtttcgtctg
      301 ccaaaaccgc gcgcaagagt gcgttgttga tggcgtgtcc cgatttgtag ccttcaaatg
      361 cgccgacaat cggatgtccg acgatataca aatcaccgat ggcatcaagg attttgtggc
      421 gcacaaactc atcgggatag cgcaagcctt caggattcag gacatccgtg tcgtcaatca
      481 cgatggcgtt gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc atttccactt
      541 cgtgcataaa gccgaaagtg cgcgcgcgcg cgatttcgtc gatgtaggat ttgccggcga
      601 aatcgatttc aaaagtgggc gagctgcggt tgaaaaccgg atggtcgaat tcgatggtca
      661 gcgttacctt aaagccgtca tacggcgtaa agcgcaccca tttgcccgct tctttgattt
      721 cgacaggctt gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc acgcccgcat
      781 cttgcaaaag gtaaataaac ggcaggctgg agccgtccat aatcgggatt tcgggcgcgt
      841 tcagctcaat cagcgcattg tcgatgccgt aggcggacag cgcggacata atgtgttcga
      901 tcgtgccgac gcgcacgcct ttgtcggtaa cgatggtgga ggaaaggcgg gtatcgttga
      961 tcaaataagg ggtcagcttg atttgttcgc ccatctcgcc gtccaaatcg gtacggcgga
     1021 aggaaatccc gctgttttca ggcgcggggt gcagggtcag cgcgacgcgt tcgcccgaat
     1081 gcagcccgac gccggtaacg ctgatggatt tcgccaaagt tctttgcagc ataaaccgct
     1141 tccttatcaa gggggtaagt tttggaataa tacgataaaa ccggaaaaac aggctatgtt
     1201 tttccatagt atttgccaat gtatccgttt tcaatacgta agccgcataa aaatgtatag
     1261 tggattaaca aaaatcaaga caaggcgacg aacccacccc cctcctgaaa aacgcaaaaa
     1321 atgccgtccg aaaacctttc ggacggcatt ttcgcgtaaa ccgtcattcc cacaaggaca
     1381 aaaaaccaaa acagaaaacc aaaaacagca acctaaaat
//
LOCUS       DAWISC010000241         1360 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWISC010000241 DAWISC010000000
VERSION     DAWISC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.241"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            254..502
                     /locus_tag="WIW87_001988"
     CDS             254..502
                     /locus_tag="WIW87_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175090.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          424 a          192 c          297 g          447 t
ORIGIN      
        1 agtttattgt ctttaaatga gataacagat aaaaccttat ttaaagaaaa tgtagtttat
       61 cgtttcagta agtattaggt gtgattttta ttaagttatt tgaggttttc ggtaagtgta
      121 gatataaggt tgagttataa ccaatcattc aggctctgtt aggcttggca actcaacctg
      181 tgcttgctga aattgggtat acagttatac gttttaagcg ggcttcgctc cctttatcat
      241 ttaagggtaa tttatgaata ttaaaaatga aatagttgct ttaatttcta aaaaaattga
      301 tggggggcaa tatagttctt tagaaaattt tgctttatcc gtactaaggg aaaaaattat
      361 aaaaaatact gtttccagtg atgaaaaatt attgattatt aatgattttt tatcaggttt
      421 tcttgagttt gatcctgaga ccgggaaacc tgtcggggag acgcttaaaa ttgagcaaat
      481 gattgatttc tttttagtct aaatcataca taccagtgaa caagctaggc ttatatccgg
      541 cttttaaata cgattgtcag gtttatactt gggataaaat caaaaataaa gttgtttaaa
      601 ctctagtaga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acagatagta
      661 cggaaccgat tcactcggtg cttcaacacc ttagagaatc gttctctttg agctaaggcg
      721 aggcaacgcc gtactggttt ttgttaattc actatattgc atggtgcagc attaaatcag
      781 tcttctagcg ccgcaatggg aaaacataga agataaacga aatggatgat ctaatactat
      841 attttttatc aggtatattc ggaaatcaag ttgctgaatg tattataaaa aacaaccgtg
      901 agataaaagt tccctttatt gtgctttacg caatattttt tacgttgatt tatactgtgg
      961 ctttgttgtt tctcagtctg atctattggg tcaatggtgc ggagattgca tggaagggga
     1021 taggtatttt cagtatgtcg gtcagttttt gtatagtctt ctgtctttat ttgattgaca
     1081 aggcaggaag atgtaaggat aagaaacaat agggatcaga tgcggcagca aacgtagatt
     1141 gggtctaggc tcaacctgcg tttgctgcat ctttggaaga aatagtggat tcgaaaaaat
     1201 aggaagaatt ctgattaatg gtatgcgagg tgtagcagtg gggactgttg ctggtggcat
     1261 aaatggttat gcaggttcta cgggtaagaa cactgacatt cgccgttaaa taataaataa
     1321 accaacttcc aaccaaacct gctatcggtt tggttggaag
//
LOCUS       DAWISC010000242         1330 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWISC010000242 DAWISC010000000
VERSION     DAWISC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.242"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(132..305)
                     /locus_tag="WIW87_001989"
     CDS             complement(132..305)
                     /locus_tag="WIW87_001989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175091.1"
                     /translation="MAENLPFPSFIHLLFLEFAFTGRGGAPRTPGFGKPPDSAPAELL
                     IEKPFHLAAKGEC"
     gene            270..>1330
                     /locus_tag="WIW87_001990"
     CDS             270..>1330
                     /locus_tag="WIW87_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ5175092.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGQAQAQTHKYAIVMNA
                     QNLPEVKWGDQYQSLTHKSNEREVIHTSGFGLAKKSISFSFNNTDEVVAEKKDTVVFG
                     AATYLPPYGKVSGFDTAKLTERKNALDQIGTTKTGLVGYSYEGSTCSSGGCPTVAYRT
                     QFTFGNSSLAKKTNGGGLDIYEDKSRDNSPIYKLKDHPWLGVSFNLGGESSFKPKRQG
                     SLVSSFSEDVTQQNGAGSQHKDKNLVYTTDDYKSQNNKNHQDKHHAVAFYLNAKLHLL
                     DKKHIKNIVQGKTVNLGILKTRIEPTEAWKRRNSNFFNGSWTYEEKGTVSVKLKLPEV
                     KAGRCINANNPNKSTKAPSP"
BASE COUNT          400 a          311 c          331 g          288 t
ORIGIN      
        1 aggcgggaat ccagaccgtt gggcatctgc agcggtttgc taaaaaccgc tttactgtga
       61 taagtgcgca gggttagaat ggcgcggtaa ccttatatat tgtaccccgt caaaggggcg
      121 cattgctttt cttaacattc ccctttggca gccaagtgaa agggcttttc aatcagcaat
      181 tcggcgggcg cggaatcggg cggtttaccg aaccccggcg ttcgcggcgc gccgccccgt
      241 cccgtgaagg caaactcaag gaataaaaga tgaataaaac ttggaaacgg caggttttcc
      301 gccataccgc gctttatacc gccatattga tgttttccca taccggcggg ggggggcagg
      361 cgcaagcgca aacgcataaa tacgctattg taatgaacgc gcaaaatctg cccgaggtaa
      421 agtgggggga tcaatatcag tcattgacgc acaaaagcaa tgaacgcgaa gttatccata
      481 cgagtggttt tggtttggca aaaaagagca ttagtttctc attcaataat accgatgaag
      541 ttgttgctga aaaaaaagat actgtcgttt tcggcgcggc gacctacctg ccgccctacg
      601 gaaaggtttc cggttttgat accgctaagc tgaccgagcg caaaaatgcc cttgatcaga
      661 ttggtacgac caaaacgggg ctggtaggct acagctacga aggtagcaca tgctccagcg
      721 gaggttgtcc tacagttgcc tatagaaccc aatttacctt cggcaattcc agtttggcaa
      781 aaaagacaaa cggcggcggg ctggatatat acgaagacaa aagccgcgac aattcgccca
      841 tttacaaatt gaaggatcat ccttggttgg gcgtgtcttt caatttgggc ggagagagct
      901 ccttcaaacc aaagagacaa ggttctttgg tatcttcttt tagcgaggac gtgacgcagc
      961 aaaatggtgc gggcagccaa cacaaagaca aaaacctcgt ttatacgaca gacgattaca
     1021 agagtcagaa taataaaaac catcaggaca aacaccacgc cgtcgccttt tatctgaacg
     1081 ccaagctgca cctgctggat aaaaaacaca ttaaaaatat cgtgcaaggt aaaacagtta
     1141 atttgggtat cttgaaaaca cgcatcgagc cgacggaagc atggaaaaga cggaatagta
     1201 acttttttaa cggtagttgg acgtatgaag agaaaggaac agtcagcgtc aaactcaaat
     1261 tgccggaagt caaagcaggc cgctgcatca acgcaaataa ccccaataag agtaccaaag
     1321 ccccttcccc
//
LOCUS       DAWISC010000243         1328 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWISC010000243 DAWISC010000000
VERSION     DAWISC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.243"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            439..>1328
                     /locus_tag="WIW87_001991"
     CDS             439..>1328
                     /locus_tag="WIW87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ5175093.1"
                     /translation="MNERNQPEVKRNGQYSTIKDKDREREYTHQGTRGGGGSVSFNNS
                     DELVSRQSGTAVFGTATYLPPYGKVSGFDADGLEERNNAAGWIRTTRIALAGYSYADV
                     VCRSATGCPKLVYKTQFTFGNSGLAKKANGGGLDIYVDKSRDNSPIYKLKDHPWLGVS
                     FNLSAKGTANDGKTINKFDEKNSNNNLVYTTESGDISLGNRHRETTAMAYYLNAKLHL
                     LDKKQIQNITDKTVQLGVLKPSIDVRQGNTGIAGLLKFWARWDIKDTGQIPVKLGLQQ
                     VKAGRCITTPNPNPKSKAPSP"
BASE COUNT          366 a          344 c          355 g          263 t
ORIGIN      
        1 agaccgttgg gcatctgcag cggtttgcta aaaaacgctt taccgtgatc agtgtgcaaa
       61 gttaaaatgg ggaggtaagc ttttcaacca gcaattcggc gggcgcggaa tcgggcggtt
      121 tgccgaatcc cgccgtttgc ggcgcgcctg ccgccgacgg tatcccgcga agcaagattt
      181 aagggataaa atatgttcca acacacaggg cggcacataa ggcgccgccc tgattcggaa
      241 gggcttgcac ccctcccgga caaagcctga tcctgccgcc ccgaagggcg gggggtttga
      301 ccgaaaagga aatacgatga ataaaacttt aaaaaggcgg gttttccgcc ataccgcgct
      361 ttatgccgcc atcttgatgt tttcccatac cggcgggggg ggggggcgat ggcgcaaacc
      421 cataaatacg ctattatcat gaacgagcga aaccagcccg aggtaaagcg gaatgggcaa
      481 tattcaacaa taaaggacaa agacagggag cgcgaatata ctcatcaggg cacaagagga
      541 ggaggaggct ctgtctcatt caacaatagc gatgagcttg tttctcgaca aagcggtact
      601 gccgtttttg gcacagccac ctacctgccg ccctacggca aggtttccgg ttttgatgcc
      661 gacgggctgg aagagcgcaa caatgccgcc ggttggattc gtaccacccg catcgcgctg
      721 gcaggctaca gctacgccga tgtcgtatgc agaagcgcca caggctgtcc caaacttgtc
      781 tataaaaccc aatttacctt cggtaattcc ggtttggcaa aaaaggcaaa cggcggcggg
      841 ctggatatat acgtagacaa aagccgcgac aattcgccca tttacaaatt gaaggatcat
      901 ccttggttgg gcgtgtcttt caatttgagc gccaagggta ccgccaacga tggtaagaca
      961 atcaacaaat ttgatgaaaa gaatagtaat aacaacctcg tctataccac ggaaagcggc
     1021 gatatttcct tgggcaacag gcatcgcgaa acgaccgcca tggcctatta tctgaacgcc
     1081 aagctgcacc tgctggataa aaaacagatt caaaatatca ccgacaaaac agtgcagttg
     1141 ggtgtcttga agccgagcat cgatgtgcgg caaggaaata cggggattgc cggcttgcta
     1201 aagttttggg ctaggtggga cattaaagat accgggcaga ttccagtcaa gctcggcctg
     1261 cagcaagtca aagcaggccg ctgcatcacc acaccgaacc ccaatcccaa atccaaagcc
     1321 ccttcgcc
//
LOCUS       DAWISC010000244         1293 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWISC010000244 DAWISC010000000
VERSION     DAWISC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.244"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            41..373
                     /locus_tag="WIW87_001992"
     CDS             41..373
                     /locus_tag="WIW87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ5175094.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WIW87_001993"
     CDS             705..1214
                     /locus_tag="WIW87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ5175095.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          342 a          247 c          307 g          397 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatatgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcgcctt gccgtactgg tttttgttaa tcc
//
LOCUS       DAWISC010000245         1266 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWISC010000245 DAWISC010000000
VERSION     DAWISC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.245"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            322..492
                     /locus_tag="WIW87_001994"
     CDS             322..492
                     /locus_tag="WIW87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ5175096.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          391 a          353 c          278 g          244 t
ORIGIN      
        1 cccctaacgc aacaggaatt catcgaaaaa accgaaacct ccaaaccgtc attcccacgg
       61 aagtgggaat ccagtaatcg aaaaaccaca ggaatctatc gaaaaaaacc aaccccaaaa
      121 aaaccgggcg gataccgcac cattctccca aaccctgatt tatagcggat gaacaaaaat
      181 cagggcaagg cggcgaagcc gcagacagta caaatagtac gggtgcttca gtactttaga
      241 gaatcgttct cttcgagcta aggcgaggca acgccgtacc ggtttttgtt aacccactat
      301 acaaaaaaca aggaaacaca aatggcgcaa tatatgtgcg gcccctgcgg ctggatttac
      361 gatgaagaac acggcgaccc cgaacacggc atcgcccccg gaaccaagtt tgaagacatc
      421 cccgacgact ggaaatgccc cgaatgcggc gtgggcaaag aagatttcta cctgttggat
      481 ttcgtgatat agcggcagcc gcacccttag ggaaaacccc gtcatccggc gttccgcctg
      541 cggcggcagg gaacaccgtt ttatagtgga ttaacaaaaa tcatgacaag gcgacgaagc
      601 cgcagacagt acaaatagta cggaaccaat tcacttgtta aagaatcgtt ctctttgagc
      661 taaggcgagg caacgccgta ctggtttttg ttaatccact atatccgttt gcgcccgcca
      721 agccgtgttg cacagatgcg acacggcttt ttccttttcg tcgtccgaat gtaaattttc
      781 ggcaaaaaac gtcaaaaatc accccccccc ccgcatttgc gtaaaataaa gtgtaatccc
      841 tttgatcata agggaatgtt gacaattaga tcgattctcc taggctgact ttcgcaataa
      901 atggggttgt caataattgt gtatggtcgt ttgccgtccg gcggccgtca ttcccgcgca
      961 ggcgggaatc cagcaacaca acgcagcagg actttatcgg aaaaaaccga aaccccaccg
     1021 accgtcattc ccgcaaaagc gggaatccaa aaacgcaacg caacaggaat ttatcggaaa
     1081 aaaccgaaac cccaccgccg tcattcccgc aaaagcggga atccagcaac cgaaaaacca
     1141 caggaatcta tcagcaaaaa cagaaacccc accgccgtca ttcccgcgaa agcgggaatc
     1201 cagacccgtc ggcacggaaa cttaccggat aaaacagttt ccttagattc cacgtcctag
     1261 attccc
//
LOCUS       DAWISC010000246         1258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWISC010000246 DAWISC010000000
VERSION     DAWISC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.246"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(85..1188)
                     /locus_tag="WIW87_001995"
     CDS             complement(85..1188)
                     /locus_tag="WIW87_001995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ5175097.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT          285 a          349 c          341 g          283 t
ORIGIN      
        1 tttccgacag ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggtat atctccaata
       61 cgggcatcgt gttccggaaa accgtcaaat ccgcatcggc atcacaatat atttgaaatt
      121 cggattgttc ggcacggtaa acagcgtcga gcggttggca tcgccgaagg caagctgcat
      181 atcgtcggaa tggatgttgc gcaacacgtc catcagatag ccgatattga aaccgacttc
      241 gagttcgccg ccctgatagg cgatttcgat ttcttcgcgc gcttcttcct gctcgttgtt
      301 gctgcacaca acgctcaaca ggccgggttg caaaaacaat cgcgcaccgc ggaatttttc
      361 attggcaaga atcgatgcac gttccaacgc gcccaacaat tctgccctcg acaacacgaa
      421 aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg gggaatttgc cgtcgatgac
      481 cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc tgtttgtcca gcagctcgat
      541 ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg
      601 caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg
      661 gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt
      721 gagataatag cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt
      781 gaaacgctcc tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg
      841 aaaatcggcg gcaggcaggg tctgcagggc aaaacgcgat ttgcccgcct tcagcgtcag
      901 acggctgtcg tcccaatcca gcgacaccag cgcaccggca ggcagcgcgc gcaaaatatc
      961 ctgaaatttc ttggcattgg tggtgatgcg gaagtcgccc gcgccgccct cgggacccgc
     1021 agtgtcgatt tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc
     1081 aatcaggacg ttggacagga tgggcagggt gtggcggcgt tcgacgatgc cggtaacggc
     1141 ttgcaacggc ttgagcaggc tgtcgcgctc ggcttgtaaa atcaacatgt tcgctccttt
     1201 aaatcggttt gtatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagac
//
LOCUS       DAWISC010000247         1221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWISC010000247 DAWISC010000000
VERSION     DAWISC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.247"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(34..492)
                     /locus_tag="WIW87_001996"
     CDS             complement(34..492)
                     /locus_tag="WIW87_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175098.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          436 a          222 c          128 g          435 t
ORIGIN      
        1 caatcgattc cgttgtattt tcgcaagaca cgtttagtct agagtctgta tattacattt
       61 atttttaggg tctgctagcc aatttcttgt tcccttcatt attttatctt ctgaaagaaa
      121 attatttttt tccatgctat taatattaat gatatgattt ttatttaaaa taatttttcc
      181 atatgaaatt tccggtttgt aatagcaaga gtctgaagca aatccttgcc cgtcaatata
      241 agaaaaataa atgttatcat cctccttata atattctaaa accaatccgg atattatttt
      301 atcaaaattc ccttcatttc catctttcat gactaaaata taattagcag gccaaccttt
      361 atcgtaaaca tattcaaact gatagttccc gaaactctcg atatccgaac taaaaaagaa
      421 gaaaagcaaa ttaatagcca atacaaaaac aaataagaac aaaaatagca aaattttcaa
      481 cttagttaac aatatttacc tctcctttaa attcaatcct gaaaggtacc ccttaccctg
      541 ggctaccaat tatagtttcc atatttctaa atattgtttt tacattactt ttttctcccc
      601 ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta atcgatactt accattaaca
      661 tctttaatca cagatatatt tccatgtata gcacaacgtg aaaaatctga gtattatata
      721 cagttatacc ttactgaaac aaagctagct gtacatatct gtaccatatt tttactacaa
      781 acaaaaaata tttataatta ttaaggataa gataaaaacc cccaacgcca gattaattct
      841 taataatttc aaataaaaaa tatttgagat tttttctttt ccaaataata tattgaaata
      901 tttaaagtta aggtagcttg atttaattag aaaatcaaaa aaaccattaa ttaatttttc
      961 ttctttaaat attttatatg atattataaa attaaataac attgcgaaaa catatgaaat
     1021 catcaaaata ccaaatatat agtgggaatg cattattatt tagctccttc ttttggcttt
     1081 gacaaattga cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc
     1141 cctgttttcc ctgttagtat caatgattct tttcttgcaa gatgtgcacc tgcacaacca
     1201 cctaatacta aaccaccacc t
//
LOCUS       DAWISC010000248         1209 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWISC010000248 DAWISC010000000
VERSION     DAWISC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.248"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            39..462
                     /locus_tag="WIW87_001997"
                     /pseudo
     CDS             39..462
                     /locus_tag="WIW87_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="WIW87_001998"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="WIW87_001998"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ5175099.1"
                     /translation="MPWNLPIFLTWLRVLLIPVLTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNFPDFYGFNLAFIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT          289 a          290 c          296 g          334 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgccctcaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac agtttgtgca caggcggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct ataagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttca gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccccatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcctgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttgaccgac
      721 tggtttgacg gatttttggc aaggttgtgg aaacagacct cggatttcgg cgccttcctc
      781 gatcccgttg ccgacaagct gatggtcgcc gtctctttgc ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatc atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 tttaaaaccg ccgcgcaaat gctggcaatc ttcctgctgc tgctgaattt tcccgatttt
     1021 tatggattta acctcgcatt catcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg tgtctcttcg atatcggaga
     1201 atgaaatcg
//
LOCUS       DAWISC010000249         1164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWISC010000249 DAWISC010000000
VERSION     DAWISC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.249"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..>1164)
                     /locus_tag="WIW87_001999"
     CDS             complement(<1..>1164)
                     /locus_tag="WIW87_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ5175100.1"
                     /translation="RAVPYVGTALLAHDVYETFKEDIQAQGYQYDPETDKFVKGYEYS
                     NCLWYEDKRRINRTYGCYGVDSSIMRLMSDDSRFPEVKELMESQMYRLARPFWNWHKE
                     ELNKLSSLDWNNFVLNSCTFDWNGGDCVVNKGDDYRNGADFSLIRNSKYKEEMDAKKL
                     EEILSLKVDANPDKYIKETGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRD
                     SQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTSPNPEPD
                     PDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDILACDRL
                     PESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFSFE"
BASE COUNT          246 a          278 c          291 g          349 t
ORIGIN      
        1 ctcaaagctg aacgcgaact gcctgcttga atcaagcaca gtcactgtga aagtgacagg
       61 tgcgggacac tgtgcggaat cttgaaagat tcctgatttc tgaaactcta cattgacggt
      121 ttcagacggc agatttaaat cttctgccgg attggactcg ggcagcctgt cgcaagcgag
      181 aatgtcgggg aagaatttgc acaaaaggcc gccatctttg ccgtcctttc cgtctttgcc
      241 gtccctgccg tttgtgcgtc ccggaacggc gggggaatcg ggtcttgtgc cgggctgtcc
      301 gtccgtatcg ggatttgcat cgggattcaa atcggggtcg ggttcgggat tggggctcgt
      361 gccggggttc tcattggggt tcgggttgtt tgcggggttt tcggcgggcg atacttcggg
      421 cagcggctgt gcgttcggtg cttccgcgct tccgggggtc aagtcgggac gcgggattac
      481 ttgaacatcc accgtggtgt tgccttgcga atccctgccg aatgttgcga caacctgaac
      541 gggattcccg ttcctgtccg tgacgggacc catattcact tttgttccgg gtgcgacttc
      601 tactttttcg gaataaccgg gataaccggt ttcctttatg tatttgtcgg gattggcatc
      661 gactttcaac gataaaatct cttccagctt tttggcatcc atttcttctt tgtattttga
      721 attgcgaata agggaaaaat cagccccatt tctgtaatca tcacctttat tgaccacaca
      781 atctccgccg ttccaatcaa atgtgcaact atttaaaaca aaattattcc aatccaaaga
      841 acttaattta ttcagttctt ctttatgcca attccaaaac ggacgtgcca gcctatacat
      901 ttggctttcc atcaattctt tgacttcggg gaatctgctg tcatcggaca taaggcgcat
      961 aatcgaactg tcaacgccgt agcagccata ggttctatta atacgtcttt tgtcttcgta
     1021 ccaaaggcaa ttactatatt cgtagccttt tacaaatttg tcggtttcgg ggtcgtattg
     1081 gtagccttgt gcctgtatgt cttctttgaa agtttcgtat acgtcatggg ctaaaagggc
     1141 tgttccgaca taaggaactg ccct
//
LOCUS       DAWISC010000250         1151 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWISC010000250 DAWISC010000000
VERSION     DAWISC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.250"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            81..1046
                     /locus_tag="WIW87_002000"
     CDS             81..1046
                     /locus_tag="WIW87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5175101.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNLLPYLDEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          290 a          321 c          303 g          237 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg aaagattccg actctgcccc acaagccaaa gaacaggcag
      181 tttccgccgc acaatccgaa ggcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgttc
      241 aaataccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa cctgctgcct tatttggacg
      361 aatatttcaa aaccacgaaa cctgccggta cgctgtttga gccggattac gaagcactca
      421 acgcttacaa accgcagctc atcatcatcg gcagccgcgc agccaaagcg tttgacaagt
      481 tgaacgccat tgcgccgacc atcgagatga ccgccgatac cgccaacctg aaagaaagtg
      541 ccaaagaacg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaagcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtcggc tttcggcccg tcttcacgct
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt cgatgaatca attaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtccttga ccgaagcgcg gccatcggtg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acgaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tttggcggcc ggcggcgcac aagaattgct gaatgcaagc aaacaggttg
     1021 ccgacgcttt taacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcctcctgtg tttcagacgg cattgcccga tacgggcgtt atctcgggaa
     1141 tggcggcaag c
//
LOCUS       DAWISC010000251         1144 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWISC010000251 DAWISC010000000
VERSION     DAWISC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.251"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..138)
                     /locus_tag="WIW87_002001"
     CDS             complement(<1..138)
                     /locus_tag="WIW87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ5175102.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            complement(200..871)
                     /locus_tag="WIW87_002002"
     CDS             complement(200..871)
                     /locus_tag="WIW87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ5175103.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          295 a          247 c          268 g          334 t
ORIGIN      
        1 aaggtaacgg gttttttgac ggttcaggta ctgctcgatc ggctgccaat caatcacctg
       61 gtccaacttc aatagcggga aacggtcgat gtgtctggca atcatggctt gggcggtttg
      121 ctggaagaag gtgctcatgg aaaatcccct aaatgtcttg gtgggaattt aaggggtttt
      181 ggggaatttt gcaaaggtct cagtctatgc ccgatataca attttgatac acaaacttgg
      241 aaatatcggt atcgtcgccg gagcgataga atgcggacag tttttcattg aattgcggca
      301 tctgcatttc ggagatttcc aagatgccgc agccccccgc catcatcaga ccgttggcaa
      361 ataaggttgc cgttcgtttg ttgccgtccc aaaaaacctg ctgccgcata caataaagca
      421 taaaacggac ggctgcctcc gtcgtcgaac cgctttgcag tccgatattt tgtaacacgc
      481 gggccacttc aatttccttc actggattcg gggcatgacg ggaaccgtcc aatagcgtta
      541 cgccgaccga accggtacgg aaatcaccgg gtgccaaaga atcgtccttg gcaacaatgt
      601 tgttgatttt tttaaggagt gaaatatcga ccggttcgcc gtttgaaata tgcgaaatca
      661 catattgata ggcacgtttc aggttcaaga tggtttggat gtcttccagt gacgcggagg
      721 ctacattttt gccatggata atttgttcgg tctgcaacaa ggtcgtctga caattctcaa
      781 aacgactgag gttgtgaatt tgggcaacca atactttctt tgccaaaaaa atattttctt
      841 tcaaactcaa cttgtatttg tcgggaaaca cgataattcc ttaaataatt aaagcagtac
      901 gttaaatcat catcttatca tcactttcgg ggtttatgtt gcgtgcgtcc gttttctaat
      961 acggtttccc atcccgcgaa acgaggcgga ggcctgtgca aaaaacctgt tcacgaaatt
     1021 ttccgcatta acgatacgct gaaaatgcgc taaaaatgac tgtgtttgaa tatcggttga
     1081 tttcatccgt ttgtgtaacc tcgcgccggt tttgtcatga ttttgcatta tagtgaatta
     1141 aatt
//
LOCUS       DAWISC010000252         1128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWISC010000252 DAWISC010000000
VERSION     DAWISC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.252"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(231..1085)
                     /locus_tag="WIW87_002003"
     CDS             complement(231..1085)
                     /locus_tag="WIW87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ5175104.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          314 a          338 c          294 g          182 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cggaaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgacg caaagatggt
      121 cgttatttcg gataaatatc cgtctttttg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacattggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaaaccc aaatacagca gcgacaatac catcccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cggcaattag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggat
//
LOCUS       DAWISC010000253         1039 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWISC010000253 DAWISC010000000
VERSION     DAWISC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.253"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            12..1013
                     /locus_tag="WIW87_002004"
     CDS             12..1013
                     /locus_tag="WIW87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ5175105.1"
                     /translation="MIDRQTNEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
BASE COUNT          292 a          277 c          271 g          199 t
ORIGIN      
        1 ctacccaaat catgattgac agacaaacaa acgaacccaa acaaaaaacc cgaatcatcc
       61 ttgcccctat gcagggtctg gtcgatgacg tgatgcgcga cctgctgacg cgtattggcg
      121 gctacgacga atgcgtcagc gaatttgtac gcattaccca taccgtgcat tcccgatcca
      181 tatggttaaa atatgtcccc gaaatcgcca acggaaacaa aacgttttcc ggcacgccct
      241 gcaccgtcca acttttgggc agcgatgcgg acaatatggc ggcgaatgcg ctggaagccg
      301 tccgcttcgg tgcgaacaaa atcgatttga acttcggctg ccccgcgccc accgtcaaca
      361 aacacaaagg cggcgcaatc cttttaaaag agccggaact gatattccac atcgtcaaaa
      421 cgctgcgcgg acgtttgccc gcacatattc cgctcaccgc aaaaatgcgg ctcggttacg
      481 aagacaaaag ccgggctttg gaatgcgcct gtgcgattgc cgaagggggc gcatgcggac
      541 tgaccgtaca cgcgcgtacc aaagccgagg gttacgaacc gccggcgcat tgggaatgga
      601 taaggaaaat ccgagacagc gtcaatattc ccgttaccgc caacggcgac gttttcagcc
      661 tgcaagacta tatcggcatc aaaacaatca gcggctgcaa cagcgtgatg ctcggtcgcg
      721 gcgcggtcat ccgccccgat ttggcgcggc aaatcaagca atacgagaac ggcgggccgg
      781 tcaaagacac ggattttgcc gaagtttcca aatggatacg gcagtttttc gagctgtgcc
      841 tgacaaaaga ggcaaacaac aaatatccgc tggcgcggct gaaacagtgg ctgggtatga
      901 tgaagaaaga atttgcagca gcacaaaatc tgttcgaccg cgtccgaacg gttaaggatg
      961 cggacgaagt tcggaacatc ttggctgaat ttgagcgaga aatgaatact tgaatatgta
     1021 tagtggatta acaaaaacc
//
LOCUS       DAWISC010000254         1032 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWISC010000254 DAWISC010000000
VERSION     DAWISC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.254"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..550)
                     /locus_tag="WIW87_002005"
     CDS             complement(122..550)
                     /locus_tag="WIW87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ5175106.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WIW87_002006"
     CDS             complement(531..965)
                     /locus_tag="WIW87_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ5175107.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          262 a          316 c          190 g          264 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 tcttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca acattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttcg
      841 gaaacatcca tatccgcaca cgaccgcttg cccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcatctcaa acccataaaa cacaatatat agtattaagc cgatgttttt tgcgaaacgg
     1021 acagacataa aa
//
LOCUS       DAWISC010000255         1030 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWISC010000255 DAWISC010000000
VERSION     DAWISC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.255"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>1030
                     /locus_tag="WIW87_002007"
     CDS             <1..>1030
                     /locus_tag="WIW87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175108.1"
                     /translation="WMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVAAKGFSGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVW
                     QDLNQDGIS"
BASE COUNT          320 a          190 c          249 g          271 t
ORIGIN      
        1 tggatgaaaa aaggtgtgga aaacctatgg gatgatacgg tcggtaaaaa gacccgttta
       61 gagaaatttg atcgggttgc attgcaacat ttcagccaat atgtagatct aattaatgaa
      121 aataatggta gattacctaa cactagtgaa attgagagaa gttactataa agccgttacc
      181 gaaaatggtg tttcttctag tgcagctatt gatttagtta ttaatcgctc acttccggat
      241 atggcagatg gttattgggc attaggtttg gggatagaag ccgaacgtat ccacaatgag
      301 caagcagtaa ataatccgaa cggtagcgaa agggataata gaaagcagtt aatatctgct
      361 ttagataaag gatttgatgg atcttttaaa gagaagcatt ttactttttt acaatctgtg
      421 ataatggatg taacaaagtt aggtgttgaa tatacaatag atggttggca aaaaattgga
      481 ggttggggta atgggataat caatgattta tataaaagtg ttgtaaaaag agagtggact
      541 ggaatatttg agatcgttaa taataacatc aagcaattta gagatctgtt cccaaatccg
      601 gaaggctgga tcgatgatgg tcaccaatgt ttcgctcctt gggttaaaga aactaaaaaa
      661 cgcaatggca aatatcatgt ctacgacccc cttgccctag atttggacgg agacggcata
      721 gaaactgtcg ctgccaaagg cttttcaggc agcttatttg atcacaccaa caacggtatc
      781 cgcaccgcca ccggttgggt ttctgccgat gacggtctgc ttgtgcgcga tttgaacggc
      841 aacggcatca tcgacaacgg tgcggaactc ttcggcgaca ataccaaact ggcagacggt
      901 tcttttgcca aacacggcta cgcggctttg gccgaattgg attcaaacgg cgacaacatc
      961 atcaacgcgg cagacgccgc attccaatcc ctgcgtgtat ggcaggatct caaccaggac
     1021 ggcatttccc
//
LOCUS       DAWISC010000256         1012 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWISC010000256 DAWISC010000000
VERSION     DAWISC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.256"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..291
                     /locus_tag="WIW87_002008"
     CDS             <1..291
                     /locus_tag="WIW87_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175109.1"
                     /translation="VTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQSGNAVGFT
                     AAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPKG"
     gene            295..495
                     /locus_tag="WIW87_002009"
     CDS             295..495
                     /locus_tag="WIW87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175110.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGRGFQIFQFFVKSE"
     gene            732..962
                     /locus_tag="WIW87_002010"
     CDS             732..962
                     /locus_tag="WIW87_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175111.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDPSAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
BASE COUNT          320 a          184 c          230 g          278 t
ORIGIN      
        1 gtaacttatc ctgcaacttt tttaggagct aaaaaattca aaggcgaaat tgatggctct
       61 aatatcgaca cttgttccgt attggttgca acacctttgc cggcacagtc gggaaatgct
      121 gttggattca cggcagcaca aatgaagttc ggggacagta agaatttctc aaaattagag
      181 aatctcaaat acccgtgcga agttatggta acggttgaaa tgacttcgac aggtaagggc
      241 atggttcctt cattaattga ttttcaggtg gcagaaaagc cgaaaggttg atttatgaaa
      301 tttgaagaac gtttcatagt tcaagacttg gaaacgcatg actttattta tcccgatcct
      361 ttcggtgatg tggggtttac tcaaaatatt aaatcagcag gtcaatttga aagctacgaa
      421 gatgcgttga attcaggcat aaatgaaata ggcagaggat tccagatatt tcagttcttc
      481 gtaaaatcgg aataaaagaa aaacaggctc ggcgggcggt ctgtcaacct ttcacaaagc
      541 ccgcaacaaa ggaaaaatat catgaaaatg aaccttgcaa cactaattat cggctgggtg
      601 gtctgtatgt ttctttttct tttcgcaatc ctctatttta tcggctaaaa acgagattcg
      661 gaaaagactt cgtccggatg aagcaagtca agaagtcgtc ttattttaaa tatcaaaaaa
      721 ggaaaaaaac gatgaacatc gttaaaaaat acgctgtaaa agcagccttg gcagccggta
      781 tcttcacacc ggccattgtt atggcagata cctttgatcc atccgcgatt ggtacgcaag
      841 tagcgaatgt aatcatgggt ttcgtgtcaa tggtttccgc cgtgggtatg gcggccatta
      901 ccgtgattct tgcaatccaa ggcttcaaaa tggcttggag catgattaaa tctgtcaaat
      961 aaacagagtg aagaaaaagg ggcgtataaa tgggctatcg tgtcggcata aa
//
LOCUS       DAWISC010000257          998 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWISC010000257 DAWISC010000000
VERSION     DAWISC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.257"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            36..419
                     /locus_tag="WIW87_002011"
     CDS             36..419
                     /locus_tag="WIW87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5175112.1"
                     /translation="MYFFPRFPFIDIDRIRWVCAGLKAFKHYIRENGLPDLIHAHCMN
                     YAGILAFKISQKYGIPYVVTEHSSTITRGLVRPHQWQPMKNAAAHAAARLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            368..>998
                     /locus_tag="WIW87_002012"
     CDS             368..>998
                     /locus_tag="WIW87_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5175113.1"
                     /translation="MAVPPPTYWAEYSTDRLNKEKKNNKPHFVFCTVSHLRRLKGHDV
                     LLTAFARALAQCPQLRLNIGGSGQEEQRLKQQAADLGITHAVTFLGALQPEAVLDLMR
                     NSDAFVLASRTETFGVVYIEALSQGLPVIATRCGGAESIVSDGNGYLVPVDDDDALAD
                     ALIKMCEHHSDFEPARLRENCLNEFGENAVIGRLIGIFRQAIAEYGKKIP"
BASE COUNT          248 a          294 c          245 g          211 t
ORIGIN      
        1 ggcaaagcgg tttggacatc tatgcatggc acggcatgta tttcttcccc cgctttccgt
       61 tcatcgacat cgaccgcatc cgctgggtgt gtgcgggttt gaaagctttc aaacactaca
      121 tccgcgaaaa cgggctgccc gacctgattc acgcccactg tatgaactat gccggcatac
      181 ttgccttcaa gatttcccaa aaatacggca tcccctatgt cgtcacggaa cacagcagca
      241 ccattacgcg cggtttggtg cgcccgcacc aatggcagcc tatgaaaaat gcggcggcac
      301 acgccgccgc acgtctcgcc gtcagccgcc atttcgcaca cgtcctgcaa cacaaatacg
      361 gctgcgaatg gcagtacctc ccccaacata ctgggcggaa tattcaaccg accgtttgaa
      421 caaagagaaa aaaaacaaca aaccgcattt cgtgttctgc accgtctcgc accttcgccg
      481 tctcaaagga cacgatgtcc tactcactgc ctttgcccgg gcgttggcac aatgcccgca
      541 actgcgcctg aacatcggcg gcagcggaca ggaagaacag cggctgaaac agcaggcggc
      601 agacttgggc attacccatg ccgttacatt tttgggcgca ttgcagcccg aagcagtctt
      661 ggatttgatg aggaacagcg acgcattcgt ccttgccagc cgcacagaaa ccttcggcgt
      721 agtctatatc gaagcactgt cccaaggatt gcccgtcatt gcaacacgct gcggcggtgc
      781 ggaatctatt gtttcagacg gcaacggata tttggttcct gttgacgacg acgatgccct
      841 tgccgacgca ctcatcaaaa tgtgtgaaca ccactctgat ttcgaacctg cccgacttag
      901 agaaaactgt ctgaacgaat ttggcgaaaa tgccgttata ggcaggttga tcggcatttt
      961 ccgacaggca atcgcagaat acggtaagaa aataccgg
//
LOCUS       DAWISC010000258          959 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWISC010000258 DAWISC010000000
VERSION     DAWISC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.258"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WIW87_002013"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WIW87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ5175114.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          220 a          209 c          249 g          281 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgctaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt tttaaggctt cggttttggt ttttctcata atggcggttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa cccatgaaac
      901 ctgcttcgca acccgtttga acatcgggtt ggcgaagcag gcggttttta tatcctgaa
//
LOCUS       DAWISC010000259          946 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWISC010000259 DAWISC010000000
VERSION     DAWISC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.259"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(56..547)
                     /locus_tag="WIW87_002014"
     CDS             complement(56..547)
                     /locus_tag="WIW87_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5175115.1"
                     /translation="MKYRRCYRNGGTYFFTVVTDKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          240 a          233 c          231 g          242 t
ORIGIN      
        1 gcagccgatg ctctttcagt tttccgtagg tcggattctc gaatccgaca ttacttcaat
       61 cgtatccaat agaaaagtcc gcattgccgc caccccaatt atgcggataa ataccctgtt
      121 tgacataacg gtgaaacgta gaaaaccccc aatcggaaat ttgtcctaca tagccatgtt
      181 tgaccggatt gaaatgcaga taatcaaaat gccaggcaaa atcggcctca tcgcggatag
      241 tatattccca aaagcgtttt tgccaaagcc tgagattgcc gccgattaaa tattggctgt
      301 gccgcttgat ttgccgccag cgttccgaat aagcagaatc attgtccggc agccgccata
      361 tggtatgcag atggtcgggc atcaacaccc atgccaaaat ttcaaacgga taccgttcgc
      421 gcaccgccat taccgcctgc cgtaaagcca aacgcaccgc atcatcggtc aaaatcttct
      481 gccgtttatc ggttacaacc gtaaaaaagt aagtgccacc attgcggtaa caacgacggt
      541 atttcataat gttatgctcg gaatgatttt gtaggtcgga ttcttgaatt cgacattttg
      601 ggcattgctg caatggattg caatgatggg aatgttaaag gttttgtcgg atacaagtat
      661 ccgacctacg cttgctgaac cgtcattccc acggaagtgg gaatctagaa tctcagggtt
      721 tcagtcattt ccgatagatt cccgccgcgt cagggggctg gattcccgcc tgcgcgggaa
      781 tgacgggttt cgagattgcg gtgttgtcgg aacgcaactg aaccgtcatt cccacgaaag
      841 tgggaatcta gaatctcggg ggttcagtca tttccgatag attcccgccg cgtcaggggg
      901 tctagattcc cgcctgcgcg ggaatgatgg gtttcaagat tgcggt
//
LOCUS       DAWISC010000260          935 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWISC010000260 DAWISC010000000
VERSION     DAWISC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.260"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>935
                     /locus_tag="WIW87_002015"
     CDS             <1..>935
                     /locus_tag="WIW87_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ5175116.1"
                     /translation="INTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEAL
                     QYRTALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTP
                     PEGEETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLL
                     LIEKGLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVS
                     KIDAIRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYL
                     KELSDIGVLQEIVIGRDKLFIHPRLMELLRGEGNS"
BASE COUNT          271 a          249 c          233 g          182 t
ORIGIN      
        1 tgattaacac ccttcctgtt atggaagccc gtgcaagttc ggaaattgaa aacatcgtaa
       61 ccaccacgga caagctgttt caatccctgc aaatggatac ggaacggcaa gaccctgcca
      121 cgaaagaagc cctgcaatac cgcaccgccc tgtttgcagg ctatgaatca ctgacgagcc
      181 gccctttatg cacacaaacc gccatcatgg tctgcaacgc catcaagcac ccctacgaaa
      241 tggccatccg caaaacaggc ggcacagccc taaaaggagg caacagcgga aatgttgtct
      301 ataccccgcc cgaaggagaa gaaaccatac gcggcaagct ggcaaattgg gagcggttta
      361 ttcacgaaag cggcgattta gacccgctta tcatcatggc ggcggcacat taccaatttg
      421 aagccatcca tccgtttacg gacggcaacg ggcggacggg gcgcatattg aacagcctgc
      481 tattgattga aaaagggctt ttggatttgc ctattttgta tttgagccgc tacatcatcg
      541 aaaacagggc ggactattac cgcctgcttt taggcgtaac cgaacggcag gattgggaaa
      601 gctggataat ctacatctta gacggcgtag ccgataccgc cgattggacg gtatcgaaaa
      661 tagatgcgat acgccgcctg ttcgagcaga cacggcaaca catacggaca cacgcacaag
      721 gaatctacac gcacgaactg gtaaatcttc tgtttgagca gccatataca cgcattgcca
      781 acctagaagc ggcagggata gccaaacggc agacggcctc taagtacctg aaagagcttt
      841 cagacatagg tgtgctgcaa gaaatcgtca tcggcaggga caaactattc attcatccgc
      901 gcctaatgga actattgcgg ggagagggca acagc
//
LOCUS       DAWISC010000261          929 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWISC010000261 DAWISC010000000
VERSION     DAWISC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.261"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..326)
                     /locus_tag="WIW87_002016"
     CDS             complement(<1..326)
                     /locus_tag="WIW87_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ5175117.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAG"
     gene            complement(672..>929)
                     /locus_tag="WIW87_002017"
     CDS             complement(672..>929)
                     /locus_tag="WIW87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175118.1"
                     /translation="ADDYLLSSLPPTVTQDGKIIRPERVGDKWILNGKPVTLSYPECS
                     NFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
BASE COUNT          346 a          203 c          124 g          256 t
ORIGIN      
        1 cccgccaata cgccagcgcg ggaaactgat gaagatacaa ccgaactaac tttacccgta
       61 cttgatgctc cagtagatac atgcctaact tttaaatctt gagcttctga agttaaatat
      121 cttcccgatt ctctatcaaa tacacctttc caaattcttc ttcttttgtc atctgaacct
      181 cgaataccta aatatctgcc atcatcataa acttcaaccc tatcaaattt acccggagca
      241 tttactgatg ccaatgcaaa tttagataca aaaaaactaa agcataggat aatctttaaa
      301 aaatttcgcc ctaggataat aaacataaca ttttgcttca tctaatttaa ttaataatgt
      361 agaagaatca gggaattgaa tttctataaa atctctatca taacgatgaa agaaattcct
      421 aaaaatatca aatgaaaacg aagttttaaa aacccttttc taatcaattc cctataataa
      481 attaacgctt gtggtaaatc tctataaata tgtgaatcag tttgaaattt aactaaatga
      541 tattcagaaa taaacataat tcggaccttt taaaacactc taaaaagccg attctaccaa
      601 ccgttaccaa aacagccaag caacagacaa agccaagaag aaaccgagaa gaaaccagaa
      661 atctataatc atcaatcagt cccaaccttg cctatgtctt ttaaaaaatt aatcagaagc
      721 ctgaaaccgt aaatgactac aaacagaatt agaaccgtcg aaccgacata agaaccttgc
      781 tttatctgct caaaattgga acattccgga taagacaacg taaccggctt tccgttcaaa
      841 atccatttat cgcccaccct ttccggcctg atgatttttc cgtcctgggt aacagtagga
      901 ggaagggacg acaataaata gtcgtctgc
//
LOCUS       DAWISC010000262          913 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWISC010000262 DAWISC010000000
VERSION     DAWISC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.262"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            313..873
                     /locus_tag="WIW87_002018"
     CDS             313..873
                     /locus_tag="WIW87_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ5175119.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          256 a          259 c          183 g          215 t
ORIGIN      
        1 ctataccacg atgaatcctt cgcaatatct gtttatcgac ctcaattttg acaaaatacc
       61 ggatacgcgc ctttgttgct tttccatctt ccaaccaact gtaaatctca aacagccggt
      121 acacgccatg cttcagtttc ttttcctgtc ggcggattgt ttcgacaaag aattgaaaat
      181 ccatttcatg caccttaaaa tttaatctgc attcaaacct tttcactttg gaagcaccat
      241 ttatcggatg tcccttcgca ataaacaaat tttcccgata ccgccgccca tttcaaccca
      301 aacccaaaag ctatgaaaaa cctcatcgcc ttcaacaaac cctatggcgt tatctgccaa
      361 ttttcaccgc acgaaaaaca caaaagcctc aaagacttta tcaatcttcc cggcttctac
      421 cccgccggac ggctcgacac cgacagcgag gggctgctgc tgctgaccga cgacggcagg
      481 cttcaggcac aaattaccga ccccaaattc aaacacccta aaacctactg ggcgcaactg
      541 gagggcgtac ccgacgaaag ccgattggaa agcctaagaa aagggataga cttaggcggt
      601 ttcgttaccc gtccggcaag catccgcatc ttgaaacacg gagaagcaga ttcgttatgg
      661 gagcgcatcc cgccgatacg cgtccgcaaa accgttcccg atttttggat tgaaattacc
      721 atttctgagg gcaaaaaccg ccaagtcagg cgaatgaccg ccaaggcggg ctatccctgc
      781 ctgcgtctga tcagagtggc aagcggcagg ctgaaactgt ttgatttgga tttaaaaccc
      841 ggggaatggg catacgcccc gtttaaacca taatcacgtt tatctcatca tttccacaaa
      901 agtgggaatc cgg
//
LOCUS       DAWISC010000263          907 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWISC010000263 DAWISC010000000
VERSION     DAWISC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.263"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            548..>907
                     /locus_tag="WIW87_002019"
     CDS             548..>907
                     /locus_tag="WIW87_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175120.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDTMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          284 a          147 c          211 g          265 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaaaca catgcgaccc tgctggcttt
      121 ggactggcag ggcaacaaac cgcttggggc ggaggagctg gcggatttga aatcgcttta
      181 caaagactta aagaataata ttggaaatat tgtatgaaca aaaaattaaa ctatattttt
      241 atgttggact gtttagggtt ggtgatattg tttacttgta taatagctac ttttgaaaga
      301 gattatggat ttaaaatttt tactaattct aagagacctg aattttatta ttggattgga
      361 atgttttatt atggaattat ttcttgctgg tttgattatc aattaatttc aacaaaggcg
      421 aattcgtata aaagaaaagt taaacaatat aaaatttttt cagtaatatt ttcagttttg
      481 atatttattt ctactatagt aaaactttaa attttggagc aaaaatttat gagcgattca
      541 attgaatatg tattgggaac gcggtctgca catgtataag gcaagtgccg tcgtgccgac
      601 gggatatgta cgggttggga ataccgcgcc gctggtcggc gaagacacgc aacggtatgc
      661 ctctttttgg ggcgacggct acgacgtgta ccgtcagttg agatggcagc agatacccga
      721 aaaacagaga aaggcattca aaaaagccgc caaaagcaaa aagaccgtga tgtttgccgg
      781 acgggaatac ggcatatcca aacagaattt gagcgatgtt tgggatgatt ttgaagacac
      841 gatggaactg aaggcgtttc cctgcctgtc ttcgctgttt ctgaccaaat ggcataaaaa
      901 tctatat
//
LOCUS       DAWISC010000264          904 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWISC010000264 DAWISC010000000
VERSION     DAWISC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.264"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..227)
                     /locus_tag="WIW87_002020"
     CDS             complement(<1..227)
                     /locus_tag="WIW87_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ5175121.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKLKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSG"
     gene            complement(211..531)
                     /locus_tag="WIW87_002021"
     CDS             complement(211..531)
                     /locus_tag="WIW87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ5175122.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            complement(650..>904)
                     /locus_tag="WIW87_002022"
     CDS             complement(650..>904)
                     /locus_tag="WIW87_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175123.1"
                     /translation="ADDYLLSSLPPTVTQDGKIIRPERVGDKWILNGKPVTLSYPKCS
                     NYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
BASE COUNT          284 a          189 c          155 g          276 t
ORIGIN      
        1 cctgacaaga cggcggcgcg ggatacgctg gcggttatct ttgcgttgat tcgggctttt
       61 gcgcccgtgg ggatatgttc gacatttgcc gcttctgtga atttagaaaa ttcattattg
      121 atttttctat atcctgtaga ttcaaaaaac tttaatttag atggcctaaa ttttattatt
      181 cgatcatcta ttcttgctgg ttctgcaaaa ctaagagaac aacacattaa aatcggtgtt
      241 gctatcagaa aattccgagt aaataaattc atgcgaaatt tttccatttt cttctgactt
      301 cctaataaaa atattagact catcagaaaa ataaattttc caaatattat gcgttaccct
      361 cttatttaaa aaataagaga aacattctat tacgtcgtat tcttttacat ttcttacaaa
      421 ctcttcaaat tccttagacg caattaatgc catcgactgc ccaaaatact tgctggacgg
      481 ctgatattta taaagtgcca actgcgcctg cgtgataaac ggcttgttca tgtttctgtc
      541 tttcaaaggt tgttttgaaa gcctgatttt aaaacacgtc atataaatat caaagcgaca
      601 gacaaagcca ggaaaaatcc gagcaaaaac caaaaatcga caaacatcat cacgccccta
      661 ctttgcctat gtcttttaag aaattaatca gcagcctgaa gccgtaaata acgacaaaca
      721 gaattaaaac catagacccg agataagctc cggatttaac ttgttcgtaa ttcgaacatt
      781 tcggataaga cagcgtgacc ggctttccgt tcaaaatcca tttatcgccc accctttccg
      841 gcctgatgat ttttccgtcc tgggtaacag taggaggaag ggacgacaat aaatagtcgt
      901 ctgc
//
LOCUS       DAWISC010000265          867 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWISC010000265 DAWISC010000000
VERSION     DAWISC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.265"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            102..503
                     /locus_tag="WIW87_002023"
     CDS             102..503
                     /locus_tag="WIW87_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ5175124.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(566..856)
                     /locus_tag="WIW87_002024"
     CDS             complement(566..856)
                     /locus_tag="WIW87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175125.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT          261 a          270 c          179 g          157 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatactact gattcggcaa aaaaatcttt cacccgctac
       61 gcaaaatcaa caacaaggtt tcaaacatga aggaaacacc aatgaacaca acccgactgc
      121 cgaccgccct cgtcttgggc tgcttctgcg ccgccgcttc tgccgccgac aacagcatca
      181 tgacaaaagg gcaaaaagtg tacgaatcca actgcgtcgc ctgccacggc aaaaagggcg
      241 aaggccgcgg aaccatgttt ccgccgctct accgctccga cttcatcatg aaaaaaccgc
      301 aggtgctgct gcacagcatg gtcaaaggca tcaacggtac aatcaaagtc aacggcaaaa
      361 cctacaacgg attcatgccc gcaaccgcca tcagcgatgc ggacattgcc gccgtcgcca
      421 cttatatcat gaacgccttt gacaacggcg gcggaagcgt taccgaaaaa gacgtaaaac
      481 aggcaaaaag caaaaaaaac taaacagaca aaatgccgtc tgaaaccggc aatccggctt
      541 cagacggcat tcaaatcaaa cctttctaac gcagcacttt cagccccata gacccgatat
      601 tgccgccacc gtcaacctcc tttgcggtca gcataaagcc gccgatgtca atacggccgc
      661 actcgaccgg ctcctgactg tcggaacgct tatcgaaaag ctcggcaagg ctcaaaccct
      721 cttcgcccgc ttccagcctc aaaccgtaag caagtgccaa atcacccgaa cgtgctgccg
      781 gcgaaacgac aaactcgccg aagaaatcaa aatgctcacg gacgccgata cccgtctcgg
      841 taaagtattt cgccatttta tagtgaa
//
LOCUS       DAWISC010000266          751 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWISC010000266 DAWISC010000000
VERSION     DAWISC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.266"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..180)
                     /locus_tag="WIW87_002025"
                     /pseudo
     CDS             complement(1..180)
                     /locus_tag="WIW87_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Caudovirus prohead protease"
     gene            453..659
                     /locus_tag="WIW87_002026"
     CDS             453..659
                     /locus_tag="WIW87_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175126.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          212 a          139 c          206 g          194 t
ORIGIN      
        1 cccagccaaa ccggtgctgc ccgcggataa aaaatagggg aacctgtaaa ggaaagggca
       61 tcggccgccg tcggtgcttt tttgtttgga agggaaatgg ctaaaatatg tagcattatg
      121 ttctgtatcg ttgtttaccg cttccgcacc tttgtccgcc ttaaagcagg tagacaccgc
      181 aatgaatcga cgcaaagaaa atgccgtctg aacatgcgtt cgggcggcgt tttgttgggg
      241 ggtatcggag cggaacgtct gaaaaagggt ttcaggcggt ctttgggcgt gtggtgacag
      301 tcgaaaacgt gataaggcta cctgaaaagt ttgggagatt ttcaggtagc ctttggtatt
      361 gggcgcaaca gacgcaggta cagattaggc ggtgtgccgt aatcgtacga atgccgattc
      421 aacctaagca gacatcagta tttaggaagt ggatgtttga tggagcaaag gttgtacgaa
      481 gggtggaagg caacctgtgg gtgtttggta tggtcgcgct tgaaaaaacg tgttttaagg
      541 gacaaatgcc gtctgaaaat cggtttcaga cggcattttc tgtttattta aagcaaacag
      601 gaaaaggcag caatattctg cagtcttcct attcacacaa gcgttttata gttaattaaa
      661 aacaaaatag tacaatactc aactttgaag gtctaaccat ggcatactct gcggacttaa
      721 gaaacaaagc tttaaactag gggctgtact a
//
LOCUS       DAWISC010000267          738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWISC010000267 DAWISC010000000
VERSION     DAWISC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.267"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          219 a          186 c          186 g          147 t
ORIGIN      
        1 cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga atctatcgga aaaaacagaa
       61 ccccccgacc gtcattcccg cgaacgcggg aatctagaac gtagaatctg agaaaccgtt
      121 ttactcgata aatttccgtg ccgacaggtc tggattcccg ccttcgcggg aatgacggca
      181 tcagtttgca ggattcggcg gaaacggtaa aaacggcaga atcgatggga tgcggcaggc
      241 tgaagcccac caaaacacaa aaattccgat gccgtctgaa atttcgtcat tcccgtgaaa
      301 acgggaatcc agccccgtgg gagcagaaac ttacaccccg tcattcccgc aaaagcggga
      361 atccagtaac cgaaaaacca cgggaatcta tcggaaaaaa cagaaccccc cgccgccgtc
      421 attcccgcga acgcgggaat ctagaacgta gaatctgaga aaccgtttta ctcgataaat
      481 ttccatgccg aggggtctgg attcccgcgt tcgcgggaat gacggcatat tttttgcatt
      541 tgatataaag ggtcgtttga attttgttca gcaagtgcaa agtgttgcac ataaaagggc
      601 gcaggataga ggcaaagcgg gcgtaggtcg ggctgtagca actgtatttt tcaccccgtc
      661 gggcaaaaat atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag
      721 agaacgattc tctaaggt
//
LOCUS       DAWISC010000268          729 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWISC010000268 DAWISC010000000
VERSION     DAWISC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.268"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            142..576
                     /locus_tag="WIW87_002027"
     CDS             142..576
                     /locus_tag="WIW87_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ5175127.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..>729)
                     /locus_tag="WIW87_002028"
     CDS             complement(624..>729)
                     /locus_tag="WIW87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ5175128.1"
                     /translation="LRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
BASE COUNT          219 a          206 c          170 g          134 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaa
//
LOCUS       DAWISC010000269          725 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWISC010000269 DAWISC010000000
VERSION     DAWISC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.269"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(128..>725)
                     /locus_tag="WIW87_002029"
     CDS             complement(128..>725)
                     /locus_tag="WIW87_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ5175129.1"
                     /translation="LSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          166 a          209 c          193 g          157 t
ORIGIN      
        1 caaacacagt caaacaaatt tatatgcccc atcccttccg aataatttga aaacacagcc
       61 gccaaaaaca aaaatgccgt ctgaaaacct ttcagacggc atttccaact tgatttcagg
      121 cagaaagtca gaacgcgata tagctgttcg ggttaaccgg tttgccgttt tgacgcacct
      181 cgaaatgaag ctgcgttctg gaagcatcgg tattgcccat caaagcaacc tgctgaccgc
      241 gtttgacctg ctgcccctcg ccgaccagca atttttggtt gtgcccgtat gcggtcagga
      301 aagaagaatt atgctggatg atgaccaagt ttccgtatcc cctcaaacct gaaccggcat
      361 aaaccacttt gccgtcagcc gccgccaaaa cgggctgtcc cgcattaccg gcaatatcga
      421 cacccttgtt gttgccgccg aaatcggcaa ccactttacc ttgcgtcgga cgctgccaaa
      481 caatgccgcc gaccgaacgc gtgccggaag gcgaagcggc aggagattgc ggggcgggcg
      541 cgggaaccgc tttattttcc gcagcgggcg cggcaggttg cggcgcggac tgcacaggcg
      601 gttgcgcggc gggtttcaca ggggtttgca cggcagccgg tacggcgggc ctgctttcta
      661 cggctgcggt tttcggtgcg gcatatcctg ccggtttgac tttaacaatc tgaccgatgc
      721 tcaac
//
LOCUS       DAWISC010000270          706 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWISC010000270 DAWISC010000000
VERSION     DAWISC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.270"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(90..641)
                     /locus_tag="WIW87_002030"
     CDS             complement(90..641)
                     /locus_tag="WIW87_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175130.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          198 a          277 c          134 g           97 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaacaaggg gctgtcctag ataactagga caaact
//
LOCUS       DAWISC010000271          692 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWISC010000271 DAWISC010000000
VERSION     DAWISC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.271"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            232..531
                     /locus_tag="WIW87_002031"
     CDS             232..531
                     /locus_tag="WIW87_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ5175131.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            528..>692
                     /locus_tag="WIW87_002032"
     CDS             528..>692
                     /locus_tag="WIW87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ5175132.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATVK"
BASE COUNT          181 a          198 c          142 g          171 t
ORIGIN      
        1 tcaaacatca aattggactg accaatcagg gcggattcta atgacacgcc gttcccgccg
       61 tcaacggcat ttacctcgca ccgcccccga aacacaagaa aaaactacac caaactacaa
      121 tttttgttca tgcaaatatt tgttttgaca ggatttaaac aaaagctccg attcaaatct
      181 gccgaaccgc ccaaaaatat attgacctaa atattaaagt ttcgtaaagt aatgcaacgt
      241 tgctttaatt ggtttgacca ctattgccga cgattagaaa aatattttcg gagatgttca
      301 attatggaaa cttgggttca aaactacacg gcaatcggcg gcagcctgta tctgactgcc
      361 gccgccgcac tcttacccat cgtctttttc tttgccgcgc tgaccgtcct gaagctgaaa
      421 ggctatcagg cggggcttta tacgctgctg attgcgcttg ccgttgccgt attcggcttc
      481 gggatgccga cgggtatggc ggtttcttcc ctgccgccgc agccgcattg acccaacgcc
      541 cctacgccac actgtatttg accgcgcatt acaaaatcgg caaatccacc cgcatcggtt
      601 tggactttga aaacgtgttc aacaaacgct accgccctat gcccgacatt cacgtttacg
      661 gcacgccgcg cagcctgacc gcaaccgtca aa
//
LOCUS       DAWISC010000272          662 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWISC010000272 DAWISC010000000
VERSION     DAWISC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.272"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WIW87_002033"
     CDS             complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WIW87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ5175133.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          212 c          120 g          135 t
ORIGIN      
        1 actatatttt caaaccggaa aaatgccgtc tgaaccttca gacggcattc cattcatatt
       61 tattcgtctt ttttgtgttc gatggcgttt cgggaacgat tgccgccgta aggctcttca
      121 accgtatatt ctccctcgat aatatcgtca tcgcgggaaa agccctcttt tctgcccgat
      181 tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca acactgcccc tcccttaaac
      241 ggcagcagca gcaataccgc caacaccgag gatacgaatc ccggactcat cagacacaca
      301 gccgccaccg tataacggat aggccacaac atctgataaa cggataccct cccgccgctt
      361 ctcattgccg cgcccgccaa taaaagaccg gacagccccg tatgcctgag catcagcacg
      421 ccggcggcaa aacctgccgc catcaaaaac aacgtccagc cgccgcccag ccaatcggca
      481 acccacacaa tcgacataat ctccaaaaac agcagcacca aaaaaccgat accgaaaaat
      541 ctcattgacc gtcatcctta tatttaagta aacagcaaac cgcccgaaca ggactccaag
      601 cgagctgcct gtaaatgatt acaaaaccat gtgcttcaag ccgaaacaat gtgaaatcac
      661 gc
//
LOCUS       DAWISC010000273          653 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWISC010000273 DAWISC010000000
VERSION     DAWISC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.273"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          170 a          196 c          140 g          147 t
ORIGIN      
        1 tattgctgtt gttctaaggt ctggattccc gcctgcgcgg gaatgacgaa tccatcctca
       61 cggaaacctg caccgcgtca ttcccgcgaa cctacattcc gtcattccca cgaaagtggg
      121 aatccagaat ctcggacttt cagataatct ttgaatattg ctgttattct aaggtctaga
      181 ttcccgcctg cgcgggaatg acgaacccat ccatacggaa acctgcaccg cgtcattccc
      241 acgaacctac atcccgtcat tcccacgaaa gtgggaatcc aggacgcgga atctcaagaa
      301 accgtttacc cgataagttt ccgtgccgac agacctagat tcccgcctgc gcgggaatga
      361 cggtttagaa gttgcccgaa acctcaaaaa aaaaaaaccg aaaccgaaca agccggattc
      421 ccgcctgcgc gggaatgacg aacccatcca tacggaaacc tgcaccgcgt cattcccacg
      481 aacctacatt ccgtcattcc cacgaaagtg ggaatctaga atctctaaag cttcagctaa
      541 cctttgaata ttgctgttgt tctaaggtct agattcccgc ctgcgcggga atgacggcgg
      601 agcggtttct gttgctcccg ataaatgccg caatctcaaa tcccgtcatt ccc
//
LOCUS       DAWISC010000274          600 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWISC010000274 DAWISC010000000
VERSION     DAWISC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.274"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>600
                     /locus_tag="WIW87_002034"
     CDS             <1..>600
                     /locus_tag="WIW87_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5175134.1"
                     /translation="STGGLEIPAAKAIHRAGIAVIIANPRQTHQFAQSQSLTKTDAKD
                     AKMPAFFAQMKAQKEDWQTMPYHPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHE
                     TQVGSVKQLIAHFDRLIDESDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLP
                     ELGRLSHKRIASLVGIAPHPRKSGEAKFKSRCFGGRSAV"
BASE COUNT          169 a          173 c          161 g           97 t
ORIGIN      
        1 aaagtaccgg aggtctcgaa atccccgccg ccaaagccat ccaccgagca ggcatagccg
       61 tgattatcgc caacccgcgt cagacgcatc agtttgccca atcgcagtcg ctgaccaaaa
      121 ccgacgccaa agatgccaaa atgcctgcct tcttcgcaca gatgaaggca cagaaagaag
      181 attggcaaac catgccctac cacccgccca cagaagcgga agaagtgttg gaagccttgg
      241 ttaaccgccg caaccaatcg gcggatatgc ggactgccga gaaaaaccgt ctgcatcagg
      301 ttcacgaaac gcaagtcgga agcgtcaaac aactgattgc ccattttgac cggctgattg
      361 acgaatcgga caaacaaatc gacgaccaca cccacacgca ttttgacggc aaagcccaag
      421 tggcggagca aatcaaaggc atcggttcga taacgacggc tacgctgatg gcgatgttgc
      481 ctgaattggg gcggctgtcg cacaaacgga tagcgagttt ggtcggcatt gccccgcacc
      541 cgaggaagag cggggaagcc aaattcaaaa gccgctgctt tggcggaagg tctgcggtgc
//
LOCUS       DAWISC010000275          594 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWISC010000275 DAWISC010000000
VERSION     DAWISC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.275"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            261..545
                     /locus_tag="WIW87_002035"
     CDS             261..545
                     /locus_tag="WIW87_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5175135.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          165 a           99 c          158 g          172 t
ORIGIN      
        1 atttgagatt gcggcattta tcggaaaaaa cagaaaccgc tccgccgtca ttcccgcgca
       61 ggcgggaatc taggtttgtc ggtgcggaaa cttatcgggt aaaacggttt ctttagattt
      121 tgcgttctag attcccactt tcgcgggaat gacgaagagt tgcgggaatg atggaaagct
      181 atgggaataa cgaagggtta aagtaatcac gggatggtgt tcgcgggaat ataaattgaa
      241 ataattcaaa agggtattat atgcagcctg cggtttatat tttagcaagc caacgtaatg
      301 gcacgttata cattggcgtt acatctgatt tggtgcaacg tatttaccaa catagggagc
      361 atttgattga gggatttaca tcacggtaca acgttactat gctggtttgg tatgaactgc
      421 atcctacgat ggagagtgca attactcggg aaaaacagtt gaagaaatgg aacagggctt
      481 ggaaattgca actgattgaa gaaaataatg tttcttggca ggatttatgg tttgatatta
      541 tttagcccgt cattcccgcg caggcgggaa tccggcttgt tcggtttcgg tttt
//
LOCUS       DAWISC010000276          585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWISC010000276 DAWISC010000000
VERSION     DAWISC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.276"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            100..387
                     /locus_tag="WIW87_002036"
     CDS             100..387
                     /locus_tag="WIW87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175136.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          199 a           70 c           99 g          217 t
ORIGIN      
        1 ccaaagaggc tgaagggtaa gagacgctta tatcatagat atagtggcaa aacaggtaat
       61 gaattaaaga cattacctgt ttttacagaa atttttaaaa tggatgattt gattgcagct
      121 tttttaggct atttatcagg taaatataga gaaggaaaaa taagcaggtt aggaatatcc
      181 ctaacggcag gaagcatttt ctttgcagta ttctttattc atgaactgat aatttttatt
      241 gttgatggaa ataaggaaaa tctttggaat tttattactg agatgatgaa agatttattt
      301 ttattttcat gttctgtttg tttattattc tacttcttat tatttattgc ggaaactatt
      361 gggaaaacaa tcaataaaat taaataagat atttaagccg ctaaaaacca tttaagtatt
      421 ttatcttcta tgcggcattt atcttggaat tgaaatactg caatcgtgag gctaaggctt
      481 ccgttgtaac gtttaccata tatttcatta ttttaatttc atcaaaaaac aaaattcaag
      541 attatacata ccgccaatag tttcttgatt gttttgtata gtgga
//
LOCUS       DAWISC010000277          582 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWISC010000277 DAWISC010000000
VERSION     DAWISC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.277"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            4..236
                     /locus_tag="WIW87_002037"
                     /pseudo
     CDS             4..236
                     /locus_tag="WIW87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            303..>582
                     /locus_tag="WIW87_002038"
     CDS             303..>582
                     /locus_tag="WIW87_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5175137.1"
                     /translation="MADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVGKQL
                     TDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFY"
BASE COUNT          177 a          140 c          134 g          131 t
ORIGIN      
        1 aaaatgttga aaacaaaaag aactaattgc ttgaaaaaag atttaattgg aatgcgggta
       61 tttcccacac tttctccatt tattcatcct cattatagga agaaaaacat gaaaaaaccc
      121 tgcttgcact ctcacttgca gccgtgtccg ccgttacggt agccgatatt cctgagtcta
      181 acggcaaggg caaagccatc atcggcgata aggaaaaaca ccgttaccaa gcatgaaaac
      241 cttgttttga aagtctatgg cgaaatccgc caagatttgg gggacggata atccctgacc
      301 ccttggcgga tgcatatgca gatttgcgcg gcaaaaccaa agtgatgacg acccagatgg
      361 gtgcttcccg cgatgtcagc aaaagcgcca aaggttggag tgtcggtatc ggtctgaatg
      421 taggcaaaca gttgaccgac agcgtcggtc tcgagtttga tccatactac cgtcacaaaa
      481 caatctgcaa accccgtgag attgtcttgg acggtgacaa aaccaaaatg ggccgctcca
      541 aatccaacga gtacggcttc cgcgtagccg caacgttcta ta
//
LOCUS       DAWISC010000278          572 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWISC010000278 DAWISC010000000
VERSION     DAWISC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.278"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>572
                     /locus_tag="WIW87_002039"
     CDS             <1..>572
                     /locus_tag="WIW87_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ5175138.1"
                     /translation="ITHDYPKATGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGW
                     RIAADYASYRKWNNNKYSVNTKELQKNNSSGIWQELKTENQENGTFHAASSLGLSAIY
                     DFKLNDKFDKFKPYIGARVAYGHVKHQVHSVRKETTTTFSPPAQGATVPGKIVEGPFS
                     KPAYHESHSTSSLGLGVIAGVGFDITPKLT"
BASE COUNT          181 a          150 c          123 g          118 t
ORIGIN      
        1 attacccacg attatccgaa agcaaccggt acagacaaag acaaaataag cacagtaagc
       61 gattatttca gaaacatccg tgcgcattcc atccaccccc gagtgtcagt cggctacgat
      121 ttcggcggct ggaggatagc ggcagattat gccagttaca gaaaatggaa caacaataaa
      181 tattccgtaa acacaaaaga gttgcaaaaa aacaatagca gtggcatctg gcaagaactg
      241 aagacggaaa atcaggaaaa cggtacattc cacgccgctt cttctctcgg cttatccgcc
      301 atttacgatt tcaaactcaa cgataaattc gataaattca aaccctatat cggtgcgcgc
      361 gtcgcctacg gacacgttaa acatcaggtt cattcggtga gaaaagaaac cacgactact
      421 ttcagtccac cagcgcaagg cgctacagtg ccaggcaaga tcgtagaagg tccgttcagc
      481 aaacctgcct atcacgaaag ccacagcacc agcagcttgg gtcttggtgt catcgccggt
      541 gtcggtttcg acatcacgcc caagctgact tt
//
LOCUS       DAWISC010000279          569 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWISC010000279 DAWISC010000000
VERSION     DAWISC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.279"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            495..>569
                     /locus_tag="WIW87_002040"
     CDS             495..>569
                     /locus_tag="WIW87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ5175139.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESK"
BASE COUNT          175 a          108 c          113 g          173 t
ORIGIN      
        1 aaattgcctt agcattgaat gtctagattc ccgcctgcgc gggaatgacg ggatttgaga
       61 tttctgtttt tgattttctg ttttcgtagg aatgacggtt taaaagttac ccgaaacctc
      121 aaaaaaaaag gctgtgtttt aatatagtgt tgatagacgt acttggcttc catgtattca
      181 atcgtggaaa tctatatctt cgtcctcgcc gaaatagtct atgcccgata tacaattttg
      241 atacacaaac ttggaaatat gggtatcgtc gccggagcga tagaatgcgg acagttatat
      301 atatacggtt tttttaggga agcgggcaga tgaagggcgg gcggattttg aattaacccc
      361 atgcaaattg acttttgcgg gcggttttac ctcccatata cttacaaaag ccaaattttt
      421 aaacatatat ccttgatata tacacggcgt aaacatatac tggaaacatc tttaaatttt
      481 ccgaaatttt aaatatgagc aactggaaac ccaatattcc ctataacgat ttaccacccc
      541 tgccgccaaa acaggatatt gaaagcaaa
//
LOCUS       DAWISC010000280          555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36892
            SAMN14500670-rid9846493.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWISC010000280 DAWISC010000000
VERSION     DAWISC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500670
            Sequence Read Archive: SRR11455044
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739161.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739161.1
            Genome Coverage       :: 353x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 23:22:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,993
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 42 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500670-rid9846493.denovo.280"
                     /isolate="M36892"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            95..>555
                     /gene="mafA"
                     /locus_tag="WIW87_002041"
     CDS             95..>555
                     /gene="mafA"
                     /locus_tag="WIW87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ5175140.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYR"
BASE COUNT          134 a          215 c          114 g           92 t
ORIGIN      
        1 tccgccgctc agacggcatt ttcgcccctt ttgtttacaa acccttaaaa tccctttaca
       61 ctcaaaatcc gttcaacatc aaacaaaccc cgctatgaaa accctgctcc tcctcatccc
      121 cctcgtcctc acagcctgcg gcacactgac cggcataccc gcccacggcg gcggcaaacg
      181 ctttgccgtc gaacaagaac tcgtcgccgc atcgtcccgc gccgccgtca aagaaatgga
      241 tttgtccgcc ctaaaaggac gcaaagccgc cctttacgtc tccgttatgg gcgaccaagg
      301 ttcgggcaac ataagcggcg gacgctactc tatcgacgca ctgatacgcg gcggctacca
      361 caacaacccc gaaagtgcca cccaatacag ctaccccgcc tacgacacta ccgccaccac
      421 caaatccgac gcgctctcca gcgtaaccac ttccacatcg cttttgaacg cccccgccgc
      481 cgccctgacg aaaaacagcg gacgcaaagg cgaacgctcc gccggactgt ccgtcaacgg
      541 cacgggcgac taccg
//
